LOCUS       DAOSGL010000001        27921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_1330_length_27921_cov_4.900990, whole genome shotgun sequence.
ACCESSION   DAOSGL010000001 DAOSGL010000000
VERSION     DAOSGL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1330_length_27921_cov_4.900990"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PKY68_00005"
     CDS             <1..888
                     /locus_tag="PKY68_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOH23456.1"
                     /translation="LNKKEEAKELLEAAFKLDKTDSRIFMELDQLYKKLNVPLSQRLS
                     IYGKYNNLVEDRDDLCVERITLYNLTGQYKTAYDLINSRHFHPWEGGEGKIVGQYVLS
                     RFALAEEAIEAGDYKKALQLLNETDSYPHNLGEGKLSTIAENDVEYYKGLIYSKLGDK
                     ENAAKWLQKAAKGNKEPQQAFYYNEQGPDKIYYQGMAWKLLGDLNKSNERFNALIEHG
                     KKHMNDHCVMDYFAVSLPDLAIWDADLNKLNRIHCHYVIGLGNLGLGKTAEAKKSFEE
                     VLRMDLSHIGANWFIAQIK"
     gene            912..3101
                     /locus_tag="PKY68_00010"
     CDS             912..3101
                     /locus_tag="PKY68_00010"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOH23457.1"
                     /translation="MKQFVIYLFASWFLSSFVAFSQEVTIPIETANVSMVLQTDRLNN
                     LRIVHTGKSLKSQAEYNKIFAGYNYNSEGASLNNAAFTVAGIVNFMEPAIAVVHADNN
                     NSVDLKYVKHSTSSLDDGATLTKIELKDPVYPFYVNLFYKAWEKEDVIEQWSEIRHAE
                     KGSVILNKFASANLYFYNKDYYLTSYNGAWAKEMMPVLTHLQQGIHSIESRMGTRENL
                     HVSPNFMLSLDGISTENTGSVMIGQLAWNGNFSIQFETDVYRNMHIVAGINPYQSAIE
                     LKPNTTFETPRFIYTLSFEGKGKASRNLHDWMRNYQLVDGKGGRLTLLNNWEATYFDF
                     DQDKLVSLFGGAKEIGVDMFLLDDGWFANKYPRNDDRAGLGDWQVNKKKLPDGIPFLV
                     KKAEEKGVLFGIWVEPEMVNPKSQLYEQKPDWVLRQEKRPEIYFRNQLVLDLTNPEVQ
                     DFVFGVIDNLFVENPSLAYIKWDCNAPIHNGYSKYLEKKKIPQSHLYVEYTKGLDKVL
                     QRIREKYPTVPMMLCSGGGGRTDYSLFNYFTEFWLSDNTDPVERIFMQWDYSYHYPAM
                     TMCNHVTSTSDKSLKYKIDVASMGKLGFDIRVDEMSKEDKEFCKKAVSNYDNFKDIVW
                     HGDLYRLVNPHEKDLASLMFVDEEKTRSVMFNYLTNWRYNADTNLRPIKLEGLDPAKN
                     YRVKEINKYNQDDKEGQIYSGDFLMTIGINSRVSLWNPSAVILIEAI"
     gene            3112..4128
                     /locus_tag="PKY68_00015"
     CDS             3112..4128
                     /locus_tag="PKY68_00015"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOH23458.1"
                     /translation="MKVSIQNSTHLGDREVLLFRLKNSYGAYIEVTNYGATLVSAVIP
                     DHRGELQNRILGYKNPEAYFTDPYYLGTTVGRVANRISNAKFTVGGKTYHLDKNDGKN
                     SNHGGFSELNKKIFDHEVLEDRVIFSTLSRDGEGGYPGNLDVRVSYSFTDNNEVMIDY
                     EVKSDKLTPVSLTNHTYFNLSGKQSNATDHFLKVFAEERLEFDENFIPTGKVLPLKGS
                     DGFDFRKFRQISNNMLLKNERNIRGFNTYYIAKGNPASGLKQVAELKEESSGILLEVR
                     TTMPGVQFYTGDYLGDEFLPFQGVCLEAHYFTDFVNRKSFTGSFIEKGEIWKEQIMYC
                     FKNG"
     gene            4164..7232
                     /locus_tag="PKY68_00020"
     CDS             4164..7232
                     /locus_tag="PKY68_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH23459.1"
                     /translation="MKKTLILFLLSLLFIPFQLYSQNVRITGSVVDEATGEPMVGVSV
                     GVVGTTRGSITDLNGNFALDVNIGASLKFSYIGYDDQTITVNSSAPLRVLMRDVSQEI
                     EEVVVVGAIIRKSDLTGAAARVTSEKLKELPTANINQALQGKIPGVYIEKNPKPGANA
                     AIKIRGSNSIHFGQSPIYVVDNVMMDNGIDMLNPDDIASIDVLKDASATALYGAKGAN
                     GVIVITTKKGQRAKGQKASTGNVFYDFWYGSSRFSNEMPLMSAYDLYDFRVDAYANAY
                     IDKNPTRDREKYIRDFLTHTNPVRNRAFSKEELESYADSLSYNWLDEIVRTGLEKNHS
                     LGFSGGGQNSSYYVSFGFNEQVGQLVNSGYERYTGKMNLEHDVKPWLKIGSNNSFIYS
                     KENPVATDNTFIVALRASPLLPISEEYWYMREGKIDNQSANNPLRDLNVVKDRHKIRF
                     FNSSFVSINPVKDLDIRSTFSVDALLEEDYTYNSTETTQSYKSSADGQAIQTKSKNLN
                     WQWDNTASYNTIFADVHRFSAMLGLNMSMYDNAWNSINAVGFGNDWFTYKKIDGATKK
                     EDFYVSSDFSTVSQMSYFGRLNYVYDSRYYLTATLRRDGASKFGSSNKWGTFPSVAFS
                     WNITGESFMQDQRFINNLRLRTGYGHAGNQNIPSYRYLTLFNPRSSLGSTILFNDGVL
                     GNPTLRWETQKQFNAGLDLSVWQSRINLVADYFFINNDGLLMGRTLAPSYGFTGEVDN
                     VGAMENKGVELSLDVAVIKTTDFQWNLGMTFSADKNKVTKLFDNAKEIYNLGGYSNNE
                     IQREGNLFVGYSINNIYVYEFDRIVQESDMEYVNSLNVGSRIVKPGDPLPKDRFEDGI
                     INDKDRYIMGRKDPDFYGGVTTSLSYKGLALNVITTYSAGAKRTSYLYETLMGGYGTS
                     AAHIDMLNRWTPENTDTNIPRAYSDGGRYGLGEMDWAIQDASFFRISNVTLSYSLPQK
                     WINKVYLSNLRLYTSLNNPLVFTNYKGFDPESGDWYPSSKTLVFGLNLAF"
     gene            7270..8823
                     /locus_tag="PKY68_00025"
     CDS             7270..8823
                     /locus_tag="PKY68_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH23460.1"
                     /translation="MKRIYIASILIGLLYGFTSCSDFLFEDPQTSMSTEQIFADIDNI
                     QPYLNGIYFKYRDTRVNRKGFFLLLGTDEVQQGEYQVRTEAQQSGLDKYDGFYFPENN
                     SIAQLWNVRWPVVTLSTMALSYLSEMEKDASETDLPKIKSYIGQASFFRACALFELVQ
                     YWGELPIPDLQGEQVILSGRKPLQTVYEMIESDFNKAVEYLSERASADVRIPRTWAAK
                     AMLAKMYMSALPESGFRDLAKAKILLNNIITEGGFRLVNQYADLWDGTKDVSSEILYA
                     FYFNNIWPDTNELQWYAGSRAVSGDPNCYMGGYDLGLPTKYCYEIISNGGIWEAGDLR
                     KDESIRYNFFYNGKAPASVAGFGEDQTSPHIKKYEDKRIDGEKSFYYSGKNVYLVRYA
                     DVLLLYAECLNEAGETGEAVNLINNTIRSRAWGGNLPAELKWNTAMSQDEFRTKIMDE
                     RMRELCFEGWRRMDLIRTGNFVEKVKTHNRWAKESGTIQKFHERFPIPHVEIKQNTNL
                     SENDQNPGY"
     gene            8932..10827
                     /locus_tag="PKY68_00030"
     CDS             8932..10827
                     /locus_tag="PKY68_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH23461.1"
                     /translation="MKTRILLLFTVLFTVTMSWAITPKQVEWSIIENPLTEGKLPMTA
                     SGRLIWGDYNNDGHLDAFIVAGQSADALVAELYKNNGDGTFTLVNTPDIYGVAWGSAA
                     FIDYDNDGNLDLIVCGSLDATASGALTFVYKNSGAPNYEFTEDESFTLAGVSPEGADN
                     GTHILEAFDYNNDGWMDLLVNGNAGGTWEVEAGGNGSSRIVALLKNNQGVLEVQTSPV
                     TGIGHFRPVNGGSIHTGDVNNDGYADIIVTGYHNEDDNTVTDLYINNKNGTFTHWADS
                     RNVFTGHQQGETVFLDTNADGWLDIVEVGRDVNNGWAHFANLFINNKDNTFTKVGSEI
                     SNLVGGQASISAGDINNDGLTDLFIAGWGSDASFYYNNGENVFTRVAVPDITRARGGS
                     VNLVDFNGDGNLDYSIFGYRDYGAGTPENPTWPHFFVKNMLGEGITSNAAPSAPTNLA
                     ASYTKGKYTLTWNKSTDDVTPKDAIRYNVYVKYSDGKTYSYVPAHINTGKLKVGGGVL
                     SFISSNSIELNLPKGDYTFGVQAVDQANVTSAFTTITSAQAGVDRVSLLEGARVYASN
                     NRINIVNNTSKDVSWSIVNLAGRLIEDGICHTSVEVEANSTLPSGVYLVRLSQGANIQ
                     TIKISIL"
     gene            10844..14191
                     /locus_tag="PKY68_00035"
     CDS             10844..14191
                     /locus_tag="PKY68_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH23462.1"
                     /translation="MKTKTLLTFVVVFTTMMSWATGPKEVTWAVIDTPLIEGNLPGSA
                     SGALIWGDYNNDGYLDAFMIAGQGSAVVGLFKNNGDETFTPVTTGITALNQGSAVFLD
                     YNNDGNLDLLIAGKSDVVKTILYKNSGAPDYTFEVDSANTDVLVDVYTGDNNYSSRIL
                     EAFDYNNDGWTDILVNGNHDANWINGCTRVVALYKNNNGTFELQTSPVETMGHFHGVN
                     GGSIHVSDVNNDGFDDIIVSGYLDATGKGVTNLYINRQDGTFMHYNPVDFTGHYSGET
                     VFLDVNHDGWNDIVEIGWGFANVYINNQDNTVTHIESSSSNLAGGYVSIAAGDVNNDG
                     YMDLMAVGYGSGSNVYYSTGDSSFIPVTLPEAARARQGKLNLVDFNNDNSLDFSNFGY
                     RAGDAGAGTSTWPNVFVKNALGEGIPENTPPTVPTNLAFAYENNQYTITWDKSTDDIT
                     PTDAIRYNLYVKYEDNSVFCLVPADITTGRLKVNGMKSFIQTNSVKLNLPEGKYTVGI
                     AAVDQAYASSAFATVDIIPLPKEVTWCVLDTPLTEGNLPMSASGALIWGDYNNDGYLD
                     AFMTAGQGSAVVGLFKNNGDETFTPVTTGITALNQGSAVFLDYNNDGNLDLLIAGKSD
                     VVKTILYKNSGAPDYTFEVDSANTDVLVDVYTGDNNYSSRILEAFDYNNDGWTDILVN
                     GNHDANWINGCTRVVALYKNNNGTFELQTSPVETMGHFHGVNGGSIHVSDVNNDGFDD
                     IIVSGYLDATGKGVTNLYINRQDGTFMHYNPVDFTGHYSGETVFLDVNHDGWNDIVEI
                     GWGFANVYINNQDNTVTHIESSSSNLAGGYVSIAAGDVNNDGYMDLMAVGYGSGSNVY
                     YSTGDSSFIPVTLPEAARARQGKLNLVDFNNDNSLDFSNFGYRAGDAGAGTSTWPNVF
                     VKNALGEGIPENTPPTVPTNLAFAYENNQYTITWDKSTDDITPTDAIRYNLYVKYEDN
                     SVFCLVPADITTGRLKVNGMKSFIQTNSVKLNLPEGKYTVGIAAVDQAYASSAFATVD
                     IDSGSALQIKTLDKVKVLAVDNAVLIMNNTENTLSCDILSLDGRVINSGLIHAKSSTS
                     ISMNNKGVYLVKLNDGENARIDKVIMN"
     gene            14491..16788
                     /locus_tag="PKY68_00040"
     CDS             14491..16788
                     /locus_tag="PKY68_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH23463.1"
                     /translation="MKKHKNICFVLIISLISFFHSNTYVSAVEVNVGKWRLVINETNG
                     KADIYNQNTLLIADNQCSYTVNNTTRSQEQLLARQIQINDITDAFGSGKVVTISAREN
                     SGIKVYHRYYLYDDKDYILTEFSVESTQDISSNYLAPVKTSTTTNFLPHLNNKTLFVP
                     FDNDRWVRYKAVNFGTSSTSYEVGLVFNATSKQGLVLGSIEHDTWKTGIVISTNAFND
                     VKSMEVYGGITSEETRDVLSHGKVKGKKIKSPKIFIGYFDDWRVGMETYGDANAIVAP
                     KLPWNKPKPFGWNSWGALQTNVSYTNSTQVADYIHNNLQNNNFCDTDGVVYMGLDSYW
                     DRLTLTDRIRFVKFCKARGQKGGIYWTPFVDWAKNPDRLVEGSSDVYYRDIYLYANGQ
                     PIRTTNIDAYAVDPTHPASKKRAETYISQFKLAKFEYLKLDFLTHGSLEADTYYDDTI
                     YTGIQAYNQGLKHIVDYLDNSMYINFSIAPLFPAHHAHGRRIACDAYAAMTGDMSTEY
                     TLNSLTYGWWLDHVYNYNDADNVVLNGVNIGENRARITSSVITGIFIIGDDYSETGPQ
                     IPKLRAPELLTKPEISKIVHYCKAFRPVESAEGDAAADMFITTLADTTYVASFNYTIN
                     AKPVLIDFDKIDLTKGTRYVVHELWTNTFYEKIDSWIENLPRRDVRLFKIYPRSSSEL
                     EQVGANKHFSIFPNPASNELRFNLENNEVIKSASVYSLIGNKIKNLNVADTRKIPIDD
                     LAKGTYIVKIESDRDQFYTSVFVKK"
     gene            16893..18506
                     /locus_tag="PKY68_00045"
     CDS             16893..18506
                     /locus_tag="PKY68_00045"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOH23464.1"
                     /translation="MAIGNSFSDDAIEANLYELAREDGDTIVIANLYLSASTLEMHAT
                     NAANNNSSYSYRKIVNGIRTEMPNYSIETALAEEDWDYISFQQASQFSGLYDTYFPHL
                     TNLKSYVEERATNPNVQFVFHSTWAYAQNFNATNFDYYNRDQMTMYNAIVDASHRAAE
                     EVGISIVIPAGTAVQNGRSSIIGDHFCRDGFHLELTYGRYTAACTWYEKLFNKVVVGN
                     KYRPFTVTAFQAKIAQYAAHYAVENPKKVTSLAYLVDDTEKVPFNNRINLSFGSSATQ
                     GAWNALESYTVGAGINDLKDIYSDITDVDVVVINRFSGINSEGPTTTATELNMPGDVT
                     KKSFFGNKKQFGGMATPTAVVKFSSLDKNETYDFVLFGSMMNCTDNRETYYKFSGKIP
                     ADTILYLNASNNIKRLVKAVGIAPNESGEIELEVGPGPNNDNSRGFYYITALSFAPHD
                     AASGLDDPLHFSVNAYPNPFDKHLNISPNIDIESIKMMSLDGKVIHSVNRLACGKTHQ
                     INTEKIPSGVYIVSITNNGVRDYLKVVKN"
     gene            complement(18633..19793)
                     /locus_tag="PKY68_00050"
     CDS             complement(18633..19793)
                     /locus_tag="PKY68_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOH23465.1"
                     /translation="MNDKLLSAVAKLQPEMKEWMDYMHQNPELNMDTPKTAKYIAERL
                     KSWGYDVVEGIGKTGIVASMTVGNGKKSIGMRADFDALPILEDNDLPYKSKIEGWSHL
                     CGHDSHATMLLGAGKYLATTKNFNGTVRLIFQPGEETMEGAPAMVADGLFKRFPVDAV
                     YGMHNMPGLPLGKFHFREGETMAAVDNWEIELTGKGSHGSMPELSIDPLVCGASLVMA
                     LQTIVSRNVSPWKNSVVNVGAFQSGIAGNAVPQKAILRLSIRNMEADLRIMVLDKIRK
                     ITKAQAEAFNCGYEIREGVAGAVLINTPENTRWAAKVAKATFGEDQVVENMHPYMGSE
                     DFAFMLKEKAGTYCMIGNGDTFMVHHPKYVFNQDMLSRGATYWVALAEEYLR"
     gene            20021..20785
                     /locus_tag="PKY68_00055"
     CDS             20021..20785
                     /locus_tag="PKY68_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23466.1"
                     /translation="MKRTGQVLLIFTLICTAVFAQTNEETDKSSTWKMVGNAQYVTQH
                     HWRGLGRGPLFGEAPAFEPSISFFNKNWNVGVVAGGSFDGVYKVVMPWISFSPVKNLW
                     IGVWDIYSPGKKLWTNQAKPFDFGLTTSNHFVDAMIFYQLPWLPLTLKWATIVTGKDP
                     NTEGKRNFTTYTELSYAHRWNDFSVWGGLGVTPWKGLYNADKGGVNNLELKFQYDFRL
                     YEPVTMPLFAKFAYSPLSEQFHFVAGASITIPYSFK"
     gene            20798..22255
                     /locus_tag="PKY68_00060"
     CDS             20798..22255
                     /locus_tag="PKY68_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH23467.1"
                     /translation="MQTTTYKGTDKVLIGFILAVLTFWLFANSMMNVSPVMTEKLGMD
                     ANTMNIAVSLAALFSGIFIVVIGGLADSIGRVKTFKIGLYLAIAGSLLVGLSPKGSLA
                     VPMLLTGRALQGLSAAFVMPTSLGMIKIFWEGKERQRAISLWSIGTFGGSGLSSLTGG
                     IIASSLGWRWIFFFAVIVAIIALYLTKELPENKREGAGKYKIDWGGIIVFMIAMIALQ
                     IFITNGNKLGWTNWITIVLVAITIVFGYLFVRVEKRSPYAFVDFGLFKNKIFTGATLA
                     NFFINGTSGMLIVSLTLMQRGAQFSAMEAGLLTLGFAIVVILFIRVGEKLLQRFGAKK
                     PIIWGALIICVAIILLMQTQIMTGQYVVLAVIAYSLFGLGLAFFATPATDAALSNLPD
                     SQAGSGAGIFKMASSLGTSFGVAIAAGIYTAVVSRTEPIGWLSNIIGFVGRQDNVVIR
                     QGAMLGLAFCLLLAILVIVSVSTSIPDKKKTDNVK"
     gene            22268..23722
                     /locus_tag="PKY68_00065"
     CDS             22268..23722
                     /locus_tag="PKY68_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH23468.1"
                     /translation="MTTKSVQYKGNDKLLFGIILGVLAFWLFAQTTLNINVDMAKDLG
                     MESSMMNIAVSITSLFSGIFIVVFGGLADKIGRVKMVKYGFYFSIIGSLLVGFTPVGG
                     ASGVVLILGRIFQGLSGAAIMPSSLALVKAYWEGAERQRAISLWSMGSWGGSGFAALF
                     GGLMVGIGWRYIFFGAAAISLIGLLMIKDTPESKAEVTDNKKTDFAGIITFMLSMIGL
                     QLLVTKGSEFGWTSPLGLTLILLTVAFALLFFKIEKGKDGAFIDFKLFNNLTFTGATI
                     SNFMINGTAGMLIVSLALLQVGGNLTAKEAGLLTLGYAISIVLFIRVGEKLLQRFGAR
                     KPMIWGSLIVGLAIILLMFTNVMTGTYKILAIISYTLFGLGLAFYATPSTDAALSNLP
                     ESQAGSGSGIYKMASSLGAAFGVAISAGVFATLNVGDNITWMEGVLTFVGRQDNVVVR
                     QAATIALGVNLMMVVMAIVSIMVTIPKSGGKLKK"
     gene            24066..24383
                     /locus_tag="PKY68_00070"
     CDS             24066..24383
                     /locus_tag="PKY68_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH23469.1"
                     /translation="MNNETNIVLTEAVFYILLVLHKPLHGYGIMQETEIMSGGRLKLS
                     AGTLYGALSSLLEKKWIIELPMEEGSRRKEYEITSLGKEVAKIELSRLEELVRNGNSI
                     IER"
     gene            24466..25047
                     /locus_tag="PKY68_00075"
     CDS             24466..25047
                     /locus_tag="PKY68_00075"
                     /inference="COORDINATES: protein motif:HMM:NF022641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HOH23470.1"
                     /translation="MKKKVYKLFFVWEYDKEEKWLNKMSAEGWQLIKGSSYSYLFERG
                     EPNEYEYKLELLDEEPDSLKSKNYIDFLKEANIENVGKCRNWIFLRKKTADGGFSSDN
                     KTLSHINHSLRVEEIYHTSRNTFILLLSIAFIGSFVTGYLPESAIVSFFSGFFMGIGI
                     SAAVLSLILMPIMRANFKKIKNHIRELYIVEGV"
     gene            25209..27437
                     /locus_tag="PKY68_00080"
     CDS             25209..27437
                     /locus_tag="PKY68_00080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOH23471.1"
                     /translation="MMVNAQPFAGDWKGAISVQGMELELIFHIAEDNGTYATTLDIPM
                     QGAAGIPVEKTEVEGSKLTLNMSALQARYEGTLNVESIEGTFKQMGQEFSLVLKKFES
                     KLPGNPALVSSDEELQQLVAYDAGNYKYRVEDYFSRPKASSFQLSPDGKYMSYREKED
                     GSTKRHVYVKEIATGKVTRILEEKDELIRGYFWKNNERLLYMMDQGGNEQYHIHAVNV
                     DGSNNVDLTPYEGVQAGVVSILKDQKDYVIVQMNKNNKQVFEPYKLNVVTGEMEQLYE
                     NKDIANPIQGYDFDKDGNLRGYSRLVNGVESEQWYKDLATGEFKLVKQMKWYDSFGIA
                     GFNYDGKNPDEAYVVTNLDSDKARIVLYDFKKNSIVKEVFSNPDYDVANMSRSRKRNY
                     EIDYFAYNGEKYVITPVSDFFKSFTKQMEKEFPGKEFYVVDSDDDENTFLIVVQSDKL
                     YGTYYQYDNKTKQFSLLYDLMPQLKEADMAEMRPITFTSRDGLTIHGYITLPKEALTG
                     EKVPLVVNPHGGPQGIRDEWGFNPETQLFASRGYATLQVNFRISGGYGKEFLKAGFKQ
                     VGRKVMDDVEDGVKYVIEQGWVDKDKMAIYGGSHGGYATLMGLVKTPDLYACGVDYVG
                     VSNIFTFMNSIPEYWKPYLDMLKEIWYDIDNEEEAAIAREASPAYQIDKIKSPLFVVQ
                     GANDPRVNINESDQIVEKLRAKGVDVPYMVKYNEGHGFAHEENSIDFYKAMLGFLAKY
                     LK"
     gene            27663..>27921
                     /locus_tag="PKY68_00085"
     CDS             27663..>27921
                     /locus_tag="PKY68_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23472.1"
                     /translation="MKRSKIFGLQPNAFFTGLTSFFTDTSAKMVYSVMPLFLLSIGAS
                     KTTISLIEGIAESTASLLKAISGYWSDKIGKNKPFMIIGYGI"
BASE COUNT         8458 a         5411 c         6374 g         7678 t
ORIGIN      
        1 ttgaacaaaa aagaagaggc taaagaactt ctagaagcag cgtttaagtt ggataaaaca
       61 gattcgagaa tctttatgga gttggatcag ttgtacaaaa aactgaatgt accactttca
      121 caaagacttt cgatttacgg caagtataat aatcttgttg aagacagaga tgacctttgt
      181 gtagaaagga ttacccttta taaccttaca ggtcagtata aaacggctta tgatttaata
      241 aactcccgtc atttccaccc atgggaaggt ggtgaaggta aaattgtcgg acaatacgtg
      301 ttgagcagat ttgctttggc cgaagaagct atagaggccg gagactataa aaaagcgctg
      361 caactactga acgaaacgga cagttatccc cataatttgg gagaaggaaa actttcaacc
      421 attgcggaaa atgatgtgga atattacaaa ggcctgatct attcaaaact tggggataag
      481 gaaaatgctg caaaatggct tcaaaaagct gctaaaggta ataaggaacc acagcaagct
      541 ttttactaca acgagcaagg tcccgataaa atttactatc agggaatggc atggaagctg
      601 ctcggagatt tgaacaaatc aaatgagaga tttaatgcac ttatcgaaca cggcaaaaaa
      661 cacatgaacg accattgtgt aatggattat tttgcggttt cactgcctga tttggccatt
      721 tgggatgcag atctcaataa gttgaaccga atacattgcc actacgtaat aggtttgggc
      781 aatttgggct taggaaaaac agcagaagca aaaaagtcgt tcgaagaagt gcttaggatg
      841 gacctgagtc atattggagc taactggttt atagctcaga tcaagtaaga tgtaataaaa
      901 aatctaccac catgaaacag tttgttattt atttgtttgc ttcttggttt ttatcttcgt
      961 ttgttgcttt cagtcaggaa gttacaattc cgattgagac ggcaaatgtg tcgatggttt
     1021 tacagacaga ccgcttgaat aatctaagga ttgttcatac cggcaaatca ttgaaatcac
     1081 aagctgaata caacaaaatt tttgccggct ataattacaa cagtgaagga gcctctttga
     1141 ataatgctgc gttcaccgtg gccggaatcg tcaattttat ggaaccggca atagccgttg
     1201 tacacgcaga taataacaac tcggtggatt taaagtatgt gaaacactct acttcttcat
     1261 tggatgatgg cgctacattg acaaagatag aactgaaaga ccccgtatat cccttctatg
     1321 taaatctttt ttacaaggcc tgggaaaagg aagatgtgat tgaacaatgg agtgaaatca
     1381 ggcatgccga aaaaggaagt gttatcctca acaaatttgc atccgctaat ctttattttt
     1441 acaacaagga ttattacctg acgagttata acggggcttg ggcaaaggaa atgatgcctg
     1501 tacttactca tttgcagcaa ggtattcact ctatagaatc gcgcatggga acaagggaga
     1561 atttgcacgt ttcgcctaac ttcatgcttt cattggacgg catcagtacg gaaaacacag
     1621 gctctgttat gattggccag ttggcctgga acgggaattt ctccatacaa ttcgagacgg
     1681 atgtgtaccg aaacatgcat attgtagcgg gtatcaatcc ttatcagtct gccattgaac
     1741 tcaagccgaa cacaacgttt gaaactcccc ggtttattta caccctttct tttgagggca
     1801 aaggcaaagc aagcagaaac ctgcacgact ggatgcgcaa ttaccaattg gtcgacggca
     1861 aaggcggtag attgactttg ttgaacaatt gggaagctac ctattttgat ttcgatcagg
     1921 ataagttggt tagtttattt ggcggagcaa aagaaattgg tgtagatatg tttttgctcg
     1981 atgacggatg gtttgctaac aaatatccca ggaacgacga tagggccggc ttaggtgact
     2041 ggcaggtgaa caagaaaaaa cttcccgacg gaattccctt tttggtaaaa aaagcagagg
     2101 agaaaggggt gttgtttggt atatgggtgg agcctgaaat ggtaaatccc aagagtcagc
     2161 tgtacgagca aaagccggat tgggtgttga ggcaagagaa gcgtcccgaa atatatttcc
     2221 ggaatcaatt ggttctggat cttacgaatc cggaggttca ggattttgta ttcggcgtga
     2281 ttgacaatct gtttgttgaa aacccatcct tggcgtatat caaatgggat tgcaacgccc
     2341 ctatacacaa cgggtattca aaatatttag aaaagaaaaa aattccacag tctcatcttt
     2401 acgtggaata caccaaagga ttggataagg tgctgcaaag gatcagagag aaatatccta
     2461 ccgtgcctat gatgttatgt tccggtggag gcggcagaac ggattacagc ttgttcaact
     2521 attttaccga attctggttg agcgataata ccgatccggt ggaaagaatt ttcatgcaat
     2581 gggattattc ttaccattat ccggccatga ctatgtgtaa tcatgtaaca agcacgagcg
     2641 ataaatccct gaagtacaag atagacgtgg catccatggg aaaattgggc tttgacatca
     2701 gggtggatga gatgagcaaa gaagataaag agttctgcaa aaaagccgta agcaattacg
     2761 ataacttcaa agacattgtg tggcacggag atctttacag gcttgtaaat ccacatgaaa
     2821 aggacctcgc ttccctgatg tttgtcgacg aggaaaaaac acgttcggta atgttcaatt
     2881 acttgacaaa ctggcgttat aatgccgaca ccaatttgag acccatcaag cttgaggggc
     2941 tggatcctgc taaaaattac cgggtaaaag aaatcaacaa gtacaatcag gacgataaag
     3001 aaggccaaat atactcgggt gatttcttaa tgaccatagg aattaattcg cgggtcagcc
     3061 tttggaatcc aagtgcggta atattaatag aagccatttg aaaccccgat aatgaaagta
     3121 agcattcaaa atagtactca tttaggcgat agggaggttc tcttatttcg gctgaaaaat
     3181 agctacggag cttatataga agtaacaaac tatggagcaa ccttagtttc ggcggttatt
     3241 cccgaccacc ggggggaatt gcaaaacagg attttagggt ataaaaatcc tgaagcttac
     3301 tttaccgatc catattattt agggacaacg gttggcaggg ttgccaatag aatttcaaat
     3361 gcgaagttta cagtcggtgg taaaacctat catttggata aaaacgatgg aaaaaactcc
     3421 aaccacggag gattttcaga actgaataaa aagatattcg accacgaggt gctggaagac
     3481 agagtgattt tttcaactct aagccgggat ggagaaggag gatatcccgg aaatttggat
     3541 gtaagggtat cttattcttt tacagacaat aatgaagtga tgattgatta tgaagtgaag
     3601 tccgataaac tgacccctgt cagcctgacc aatcacacct atttcaattt atcggggaag
     3661 caatcgaacg caaccgatca ttttttaaag gtttttgccg aagaacgatt agagtttgat
     3721 gagaacttta tacctacggg caaggtttta cctttaaagg gctcggatgg gtttgatttc
     3781 aggaagtttc gacagataag caataatatg cttttgaaaa acgagagaaa cataagggga
     3841 ttcaacacgt attatattgc caaaggaaac ccggcgtccg gcttgaagca agtggcggaa
     3901 ctgaaagaag aatcgtcggg aattttgttg gaagtcagga ccactatgcc cggtgtacaa
     3961 ttttataccg gtgactattt gggtgatgaa tttttacctt ttcagggagt ttgcctggaa
     4021 gctcactatt ttaccgactt tgtaaatcgt aaaagcttca ccggttcttt catcgaaaag
     4081 ggagaaatat ggaaagagca aattatgtat tgtttcaaga atggttgatt tgacctaacg
     4141 gtatcatcta aaaaaacacg atcatgaaaa agacccttat tcttttttta ttaagccttc
     4201 tttttattcc atttcaattg tacagtcaaa acgtacggat taccgggtct gttgtggatg
     4261 aggctaccgg agaacctatg gttggcgtaa gtgtaggagt tgtaggtacg acgagaggaa
     4321 gtattacgga tttaaatgga aactttgctt tagacgttaa cataggtgcg agcctgaagt
     4381 tttcttacat aggttacgat gaccaaacaa taacggtaaa cagtagtgct cctctgaggg
     4441 tgttgatgag agatgtgagc caggagatag aggaggttgt tgttgttgga gccatcatca
     4501 ggaaatcgga tttaacaggc gctgcagcac gtgtaacaag cgaaaaattg aaagaactcc
     4561 cgacagccaa catcaaccag gcgttgcaag gcaaaattcc gggagtatat atagaaaaaa
     4621 accccaagcc gggggccaat gctgcaatca aaatacgagg ctccaactcc attcactttg
     4681 gtcaaagccc catctatgtg gttgataatg tgatgatgga taatggaatc gacatgttga
     4741 atccggacga cattgcctct atcgacgttt tgaaagatgc ttcggctaca gcgttgtatg
     4801 gtgctaaagg tgcgaatggt gtgattgtca tcaccaccaa aaaagggcaa agagcgaaag
     4861 gacaaaaagc aagcacggga aatgtgtttt atgatttttg gtacggcagc agcaggtttt
     4921 ccaatgaaat gcctttgatg tctgcatatg atttgtacga ttttcgcgtg gacgcttatg
     4981 ccaatgcata catagacaag aatcccaccc gcgacagaga aaaatacata agagattttc
     5041 tgacacatac caatccggta agaaacagag ctttttcaaa agaagaattg gaatcttatg
     5101 cagacagtct ttcctataac tggctggatg aaatagtcag aaccggactg gagaaaaatc
     5161 attctttagg tttttccggc ggtggtcaaa acagcagtta ttacgtcagt tttggattta
     5221 acgagcaagt aggacaatta gtaaactccg gatatgaacg atatacaggg aaaatgaatc
     5281 tggaacacga cgtaaaaccc tggttgaaaa taggctccaa caactctttt atttattcga
     5341 aggaaaaccc cgtagccacg gacaacacat ttattgttgc cttgcgtgcc agtccgcttt
     5401 taccgatttc tgaagaatat tggtatatgc gtgaaggtaa aatcgataat caaagcgcca
     5461 acaaccctct aagagacttg aacgttgtaa aagacagaca caaaatccgt tttttcaatt
     5521 cgagctttgt cagcatcaac cccgtaaaag acttggatat tcgctccaca ttctccgttg
     5581 atgctttgtt ggaagaagat tatacctaca attcaacgga aaccactcaa tcttacaaat
     5641 cttcggctga cggtcaggcc attcagacaa aaagtaaaaa tctgaattgg caatgggata
     5701 ataccgcatc gtacaacact atttttgccg atgtccaccg attttcagcc atgcttgggt
     5761 taaatatgtc tatgtacgac aatgcctgga acagcatcaa tgctgtgggc ttcggaaacg
     5821 actggtttac ctataaaaaa atagacggag ccactaaaaa agaagatttt tacgtcagtt
     5881 ccgacttctc aaccgtatcg cagatgtctt atttcggtcg tctcaattat gtttatgatt
     5941 cccgctatta ccttacggct accttgcgca gggatggagc gtctaaattc ggctcttcca
     6001 acaaatgggg cactttccct tccgtagctt tttcgtggaa tataacaggc gaatctttca
     6061 tgcaagacca acgttttata aacaatcttc gtctgagaac gggatatgga catgccggaa
     6121 accaaaacat tccttcttat cgctacctga cgcttttcaa ccctcgaagt tctttgggat
     6181 cgaccatatt gttcaacgat ggtgttcttg gcaacccgac tttaagatgg gagactcaaa
     6241 aacaattcaa tgcagggctc gatttaagtg tgtggcaaag caggataaac ctggtcgccg
     6301 attatttttt cataaacaac gatggattgc tgatgggaag aaccctggca ccatcctacg
     6361 gcttcacggg cgaagtggat aatgtcggag caatggaaaa caaaggagtt gagttgtcgt
     6421 tagatgtggc tgtaataaaa acaaccgact ttcagtggaa tctggggatg acgttttctg
     6481 cggacaaaaa taaggttaca aagctgttcg ataacgccaa agagatatac aatttaggag
     6541 gttactcgaa caatgaaatc caacgcgaag gaaacctgtt cgtgggctat tccatcaaca
     6601 atatttatgt ttatgagttt gacaggatag ttcaggaatc ggatatggaa tacgtaaact
     6661 ccttaaatgt gggttcccgt attgtgaaac ccggagatcc cctgcccaag gatagatttg
     6721 aagatggaat aataaacgac aaagaccgct acatcatggg cagaaaagac cccgactttt
     6781 atggtggagt taccaccagc ttatcttaca aaggtcttgc tctcaacgtg atcactacct
     6841 attccgccgg tgcaaaacgc accagttatc tttacgaaac cttgatggga ggctacggta
     6901 cttccgctgc ccacatcgat atgttgaacc gatggacacc cgaaaacacc gacaccaaca
     6961 ttccacgagc ttattcggat gggggaaggt atggattggg agaaatggac tgggctattc
     7021 aggatgcttc ttttttcaga atctccaatg tgactttgtc ttacagttta ccacaaaagt
     7081 ggattaataa agtctacctg agcaacctaa ggctttacac atctttaaat aacccccttg
     7141 tatttaccaa ttacaaaggt tttgaccccg aaagcggtga ctggtatcct tcatctaaaa
     7201 cgctcgtttt tggactgaac ctggcctttt aagatttgag aatgtataat catcaaaaag
     7261 cacacgaaaa tgaaaagaat atatatcgca tcgattttga taggactcct ctacggtttc
     7321 acatcgtgta gtgatttctt attcgaagat ccgcaaacct ccatgtccac tgaacagatt
     7381 tttgcggata tagacaatat tcaaccctat ctgaacggta tttatttcaa atacagagac
     7441 accagggtta accggaaagg tttcttcttg cttttaggaa ccgacgaagt gcaacagggt
     7501 gaatatcagg taagaactga ggctcaacaa agtggcttgg ataaatacga cggtttttac
     7561 tttccggaga acaattccat cgctcagttg tggaatgtgc gttggcccgt tgttacactt
     7621 tctaccatgg ctctttcata tttgagcgag atggagaagg atgcttcgga aacagatctt
     7681 ccgaaaatca aatcttatat tggacaagcc tcttttttca gggcttgtgc attgtttgag
     7741 ttggttcagt attggggcga actgcccatt cccgatttgc agggagaaca agtaatactg
     7801 tccggcagga aaccactcca aacggtttat gaaatgattg aatccgactt caataaagca
     7861 gtggaatatt tatcggagag agcaagtgcc gatgtccgca taccaagaac ctgggctgca
     7921 aaggcgatgc tcgcaaaaat gtatatgtcg gcattgcccg aatccggctt cagggatttg
     7981 gcaaaagcaa aaatattgtt gaacaatatt ataacagaag gaggtttcag attggttaat
     8041 caatacgccg acctttggga cggaactaaa gatgtttcaa gtgaaatact ctatgctttc
     8101 tactttaata acatctggcc cgacaccaat gaattgcaat ggtacgcagg ctctcgtgcc
     8161 gtgtcgggtg acccgaattg ctacatggga ggctatgatt tagggctgcc caccaagtat
     8221 tgctacgaaa taataagtaa tggaggtatt tgggaagcgg gcgatttgcg caaagacgaa
     8281 agcatacgtt ataatttttt ctacaatggt aaagcaccgg cttcggtagc tggttttggc
     8341 gaagaccaaa cgtcaccgca catcaagaag tatgaagaca aaagaattga tggagaaaaa
     8401 tcgttttatt attcaggtaa aaacgtgtat ttggtaagat atgccgatgt cttgttgctg
     8461 tacgccgaat gcttaaatga agcaggagaa acgggagaag ctgttaacct catcaacaac
     8521 accattcgct cacgtgcctg gggcggtaat ttgcctgcag aactaaaatg gaacacagct
     8581 atgtctcaag acgagttcag aactaaaatc atggatgaac gcatgcgtga actctgcttt
     8641 gaaggttgga gacgcatgga tttgatccgc acgggaaatt ttgtggaaaa agttaagaca
     8701 cataatcgtt gggcaaaaga atcgggaact attcagaagt ttcacgagag atttcccatt
     8761 ccacacgtag agattaagca aaatacgaat ctctcggaaa acgatcagaa ccccggttac
     8821 tgagataata atgcaaataa gcttaagaat ggattaaaca cctcattgaa cgatctttat
     8881 agaaaaaaca ctcaagaatt taaaatgaat taagattaaa atttatcgct tatgaaaaca
     8941 agaattttac tactatttac ggtacttttt accgttacga tgtcgtgggc aattactccc
     9001 aagcaagtgg agtggtctat tattgaaaac ccgctgacag aaggaaaact tccgatgaca
     9061 gcatcgggac gattgatttg gggtgactac aacaacgacg gtcatcttga cgcctttata
     9121 gttgccggac aaagtgcgga tgcactggta gccgaattat ataaaaacaa tggcgacggt
     9181 acatttacat tggtaaatac acctgatatt tacggggttg cctggggatc agccgccttt
     9241 atcgattacg acaacgacgg aaatttagat ttaattgtgt gcggttctct ggatgcaaca
     9301 gcttcggggg cacttacttt cgtatataaa aactccggtg ctcccaacta tgaatttact
     9361 gaagatgagt catttacctt ggcaggagtg tctcctgaag gtgccgacaa tggaacccat
     9421 atcctggagg ccttcgacta taacaacgat ggctggatgg acttgcttgt aaacggaaac
     9481 gcgggcggca cttgggaagt tgaagccggt ggaaacggca gttctcgtat agttgcacta
     9541 ctcaaaaaca atcagggtgt tttggaagtc caaacaagtc ctgttacggg aatcggccat
     9601 ttccgtcctg tcaacggagg ttccatccat acgggagatg tgaacaacga cggatacgca
     9661 gatatcatag taaccggata ccacaatgaa gatgataata cggtaacaga tttatacatc
     9721 aacaataaaa acggaacatt tactcactgg gctgattcaa gaaatgtatt taccggacac
     9781 caacaaggag aaaccgtttt tttggataca aatgccgacg gatggctgga tatagtagag
     9841 gtaggcaggg atgtaaacaa tggttgggcg cattttgcca atttattcat caacaataag
     9901 gataacacct ttacaaaagt aggaagtgaa ataagcaatc tggtaggtgg acaagcaagt
     9961 atctccgcag gtgatattaa caacgacggt ctgactgacc ttttcatagc cgggtgggga
    10021 agtgatgcct ctttttatta caataatgga gaaaacgtat ttaccagggt agccgtaccg
    10081 gatataacca gagccagagg cggttctgtc aacttggttg atttcaatgg cgatggtaat
    10141 ttagactatt ctattttcgg ttatcgcgat tatggcgcag gaactccgga aaatccgact
    10201 tggccccatt tctttgtaaa aaatatgctg ggagagggga taacaagcaa tgcggctcca
    10261 agtgctccaa caaacttagc tgcatcttac acaaagggta aatatacatt gacctggaat
    10321 aaatcgaccg atgatgtaac tccaaaagat gccatacgct acaacgttta tgtgaaatat
    10381 tccgatggaa aaacgtattc ttatgtgccg gctcatatca acaccgggaa gttgaaagta
    10441 ggcggaggcg ttttgtcttt tatttcgagc aattctattg aattgaattt accgaaaggg
    10501 gattacacct ttggtgtaca agcagtggat caggcaaatg tcaccagcgc ctttactact
    10561 attacttccg cccaggcagg tgtggataga gtctctctcc tggaaggtgc ccgggtttac
    10621 gcttcaaaca atagaattaa tattgtaaat aatacatcga aagatgtaag ttggtctatc
    10681 gtaaaccttg caggacggtt gattgaagac ggaatttgtc acacttccgt tgaggtagaa
    10741 gcaaactcaa cacttccttc gggagtgtat ttagtgagat tgtctcaagg agctaacatt
    10801 caaactatta agatctcaat attataaatt aaattctaaa gtcatgaaaa caaaaacgtt
    10861 actaactttt gtggtagttt ttaccaccat gatgtcttgg gcaaccggtc ccaaagaagt
    10921 aacatgggct gtgattgata cccccctgat agaaggaaat cttccggggt cggcttccgg
    10981 cgctcttatt tggggcgact acaacaacga tggatacctg gatgctttta tgatagcagg
    11041 acaaggaagt gctgtagtcg ggttatttaa gaacaatggc gatgaaacct tcactcctgt
    11101 cactacgggc atcacagcac tcaatcaggg atctgccgtt ttcctggatt acaacaacga
    11161 cggaaatctg gatttattga ttgccggtaa atccgatgtg gttaaaacca tactgtacaa
    11221 aaactccggt gctccggatt acacattcga agtggacagt gccaataccg acgtcctggt
    11281 tgacgtatat acgggagata ataattacag cagtcgtatc ttggaggctt ttgattataa
    11341 caatgacgga tggaccgata ttcttgtaaa cgggaatcac gatgcaaact ggataaacgg
    11401 ttgtacccgt gtggtagctc tctacaaaaa caacaacgga acttttgagc tacagaccag
    11461 tcctgttgaa acgatggggc atttccacgg tgtcaatgga gggtctattc acgtgagtga
    11521 tgtcaataat gacggatttg atgacatcat agtatccgga tatcttgatg ccaccggtaa
    11581 gggagtaaca aacttgtaca tcaacaggca agacggcaca ttcatgcatt acaaccctgt
    11641 tgattttaca ggtcattaca gtggagaaac cgttttctta gatgttaatc acgatggttg
    11701 gaacgatatt gtagaaattg gttggggttt tgctaatgtg tatataaata atcaagacaa
    11761 cacggtaact cacattgaaa gttcaagctc aaatttagcc ggcggatatg tatccattgc
    11821 cgcaggtgat gtcaataacg acggttatat ggatttgatg gctgtgggat atgggtcagg
    11881 ctccaatgtg tactacagca cgggtgacag ttcatttatc cccgtaacct tacctgaggc
    11941 tgcaagggca agacagggta aattaaactt ggttgatttc aacaacgaca acagccttga
    12001 tttttccaac tttggatatc gggcaggcga tgccggtgcc ggcacctcta cctggcccaa
    12061 tgtttttgta aagaacgcac ttggggaggg aattccggaa aacacgccac ctacggtacc
    12121 gacaaatttg gcatttgctt acgaaaacaa ccagtatact ataacctggg ataagtcgac
    12181 tgatgacatt actcctaccg atgccatccg ctacaatctg tacgttaagt atgaagataa
    12241 ttcggtattt tgtcttgtac ccgccgatat tactaccgga aggttaaagg tgaatggtat
    12301 gaagtcgttt attcaaacca acagtgtaaa actaaatctc ccggaaggga aatacacggt
    12361 tggaattgct gccgttgatc aggcctatgc ttcgagcgct ttcgctacgg tggatattat
    12421 tcctcttccc aaagaagtga catggtgtgt tcttgatacc cccctgacag aaggaaatct
    12481 tccaatgtca gcttccggcg ctcttatttg gggcgactac aacaacgatg gatacctgga
    12541 tgcctttatg acagcgggac aaggaagtgc tgtagtcggg ttatttaaga acaatggcga
    12601 tgaaaccttc actcctgtca ctacgggcat cacagcactc aatcagggat ctgccgtttt
    12661 cctggattac aacaacgacg gaaatctgga tttattgatt gccggtaaat ccgatgtggt
    12721 taaaaccata ctgtacaaaa actccggtgc tccggattac acattcgaag tggacagtgc
    12781 caataccgac gtcctggttg acgtatatac gggagataat aattacagca gtcgtatctt
    12841 ggaggctttt gattataaca atgacggatg gaccgatatt cttgtaaacg ggaatcacga
    12901 tgcaaactgg ataaacggtt gtacccgtgt ggtagctctc tacaaaaaca acaacggaac
    12961 ttttgagcta cagaccagtc ctgttgaaac gatggggcat ttccacggtg tcaatggagg
    13021 gtctattcac gtgagtgatg tcaataatga cggatttgat gacatcatag tatccggata
    13081 tcttgatgcc accggtaagg gagtaacaaa cttgtacatc aacaggcaag acggcacatt
    13141 catgcattac aaccctgttg attttacagg tcattacagt ggagaaaccg ttttcttaga
    13201 tgttaatcac gatggttgga acgatattgt agaaattggt tggggttttg ctaatgtgta
    13261 tataaataat caagacaaca cggtaactca cattgaaagt tcaagctcaa atttagccgg
    13321 cggatatgta tccattgccg caggtgatgt caataacgac ggttatatgg atttgatggc
    13381 tgtgggatat gggtcaggct ccaatgtgta ctacagcacg ggtgacagtt catttatccc
    13441 cgtaacctta cctgaggctg caagggcaag acagggtaaa ttaaacttgg ttgatttcaa
    13501 caacgacaac agccttgatt tttccaactt tggatatcgg gcaggcgatg ccggtgccgg
    13561 cacctctacc tggcccaatg tttttgtaaa gaacgcactt ggggagggaa ttccggaaaa
    13621 cacgccacct acggtaccga caaatttggc atttgcttac gaaaacaacc agtatactat
    13681 aacctgggat aagtcgactg atgacattac tcctaccgat gccatccgct acaatctgta
    13741 cgttaagtat gaagataatt cggtattttg tcttgtaccc gccgatatta ctaccggaag
    13801 gttaaaggtg aatggtatga agtcgtttat tcaaaccaac agtgtaaaac taaatctccc
    13861 ggaagggaaa tacacggttg gaattgctgc cgttgatcag gcctatgctt cgagtgcttt
    13921 cgctacagtg gatatagatt ccggttctgc gttacaaatc aaaacattgg ataaggttaa
    13981 agttctagct gtagacaatg cggttttgat aatgaataac acggaaaaca cgctgtcgtg
    14041 cgatattctt tctctcgacg gaagagtaat caattcaggc cttattcatg cgaagtcaag
    14101 cacgtcaatt tcaatgaata ataagggagt ttatttggta aaactcaatg acggcgaaaa
    14161 tgcacgaatt gataaagtga ttatgaatta gtgaatctcc ggttaattac gggaaagtgg
    14221 caacactttc ccgtaattaa tttttattta aaaaatatcc gtaaattgcc tgacgaatct
    14281 aactcaacgg aacggcaact atttcagccc aacggcaagg ccttgggttt taagattggt
    14341 aagtacgaat tgcgccctgt aagtgcataa gtgtagaata aaattttcag cgcggtttca
    14401 tttaaaatac tatgcaaggc tctatcaaat aaattttgta aacgataatg ctaagaaaaa
    14461 aaacattgta aaatcacctt tcacaaaaaa atgaaaaaac ataaaaacat ctgtttcgta
    14521 ctaattatta gcctgataag cttttttcat tccaatacct acgtttcggc tgtggaagtc
    14581 aatgtaggaa agtggcgatt ggtgattaat gaaacaaacg gtaaagccga tatatacaat
    14641 caaaataccc tgttgattgc agacaatcaa tgctcttata ccgtaaacaa tacgacgaga
    14701 agtcaggaac agttattagc caggcaaatt cagataaacg acattaccga tgcttttggc
    14761 agcggaaaag ttgttactat ctcagcccgg gaaaacagcg ggatcaaggt ttatcatcgt
    14821 tattatcttt acgacgataa agattatatt ttaaccgaat tttcggtaga atcaacgcaa
    14881 gacatatcgt cgaattatct tgcaccggtt aaaacatcga caacgacaaa ctttttacct
    14941 catctcaaca ataaaacact ttttgttcct ttcgacaacg acagatgggt tcgctataaa
    15001 gccgtcaact ttgggacctc atctacaagt tacgaagtgg gcctggtatt taacgccact
    15061 tccaaacagg gcctggtgct tgggtctatt gagcacgata cgtggaaaac cggcatcgta
    15121 atatcaacca atgcgttcaa tgatgtgaaa tccatggagg tgtacggtgg tattacgtct
    15181 gaagaaacca gagatgtgct ttcccatggg aaggtaaaag ggaaaaaaat taaatctccc
    15241 aaaatattta tcggctactt tgatgattgg cgtgtaggaa tggaaactta cggtgatgcc
    15301 aatgccatcg tagctcccaa gcttccgtgg aataagccga agcccttcgg ttggaacagt
    15361 tggggtgcac ttcaaacgaa tgtttcatat accaactcaa cgcaagtagc tgattatatt
    15421 cataataact tgcaaaacaa taatttttgc gatacggacg gtgtggttta catggggttg
    15481 gattcttatt gggataggtt aacactaacg gacagaatca ggtttgtcaa attctgtaaa
    15541 gctcgcggac aaaaaggggg tatctattgg actccttttg tggattgggc taaaaatccc
    15601 gatcgactgg tcgaaggttc gagtgatgtt tattatagag acatctattt gtatgcaaat
    15661 ggacaaccga taagaacaac taacatagat gcatatgcag tggacccgac ccatcctgcc
    15721 agtaaaaaaa gagctgaaac ctatatttcc cagttcaaat tggccaaatt tgagtatctt
    15781 aaattggatt ttttgaccca tggctcctta gaagcggata cgtactatga cgatacaata
    15841 tataccggaa tacaggctta caatcaaggt ttaaagcata ttgtagatta tttggacaat
    15901 tcaatgtata taaacttttc catcgcaccg cttttcccgg ctcatcacgc acacgggcgc
    15961 aggatagcgt gtgatgctta cgcagctatg accggtgata tgagtacgga atacaccttg
    16021 aattcactga cctatggttg gtggctcgat catgtgtata attacaacga tgccgacaat
    16081 gtggtgttga atggcgtaaa tatcggcgaa aacagggcga gaatcacctc ttcggtgatc
    16141 acaggcatct ttatcatcgg cgatgactat agtgaaacag gtccgcaaat acccaaacta
    16201 agagctccgg aactgttaac caagccggag ataagtaaaa ttgtgcacta ttgcaaagcg
    16261 ttccgtccgg tagaatcggc agaaggcgat gccgctgccg atatgtttat caccaccctt
    16321 gcggatacca cttatgtggc ttcattcaat tataccatca atgccaaacc tgttctgatt
    16381 gattttgata aaatcgacct gaccaaaggc acacggtatg tggttcatga attgtggacc
    16441 aatactttct atgaaaaaat tgactcatgg atagaaaacc tgccaaggag agatgtccgg
    16501 ctatttaaga tttatcccag atcatcttct gaactggagc aggtaggggc aaacaagcat
    16561 ttttctattt ttcccaatcc ggcttcgaat gaacttcgtt ttaatctgga aaataatgag
    16621 gtgataaagt cagcatcggt ttactcctta attggcaaca aaatcaaaaa tctgaatgta
    16681 gctgataccc gtaaaatacc catcgatgat ttggctaaag ggacttatat tgttaaaata
    16741 gaatcggata gagaccaatt ctacacttct gtatttgtta aaaaataaca ggcttgtcaa
    16801 aataaactgc atctatgtat aaaaaattct tattaagctt cttgctgccg gccctcgttt
    16861 gtacttccct taacgcaaaa cagataaagc tgttggctat tgggaacagt ttttcggacg
    16921 atgccataga agccaatcta tatgagcttg ccagagaaga tggcgacacc atcgtcattg
    16981 ccaacctgta ccttagcgcc agcactttgg aaatgcatgc caccaacgct gccaataata
    17041 attcttctta ttcctataga aaaattgtaa acggtatacg tacggaaatg ccgaattatt
    17101 ctattgaaac agctttagcg gaagaagact gggattatat ctcttttcag caggccagcc
    17161 aattctcagg tctgtacgac acttacttcc ctcatttaac caatttaaaa tcttacgtgg
    17221 aggaacgcgc aaccaatcca aacgtacagt ttgtttttca ctccacatgg gcttatgctc
    17281 aaaacttcaa cgctaccaac ttcgattatt acaatcggga tcagatgact atgtacaatg
    17341 ccattgtcga tgcatctcac agagcagccg aagaagtcgg aatcagcatt gttattcctg
    17401 ccgggacggc cgtacaaaac ggcaggtcga gcattatagg agatcatttt tgcagggatg
    17461 gctttcacct ggaattgact tacggaagat acacggctgc ttgtacctgg tacgaaaaat
    17521 tgtttaacaa ggtggtggtt ggaaacaaat acaggccatt caccgttact gcttttcaag
    17581 caaaaatagc ccaatatgcg gctcattacg ctgtggaaaa tccgaagaaa gtcacttctt
    17641 tggcttatct ggtggacgat acggaaaaag tgccgttcaa caacaggatt aatttaagtt
    17701 ttggaagttc agcaacacag ggagcctgga atgctttgga aagctatact gtcggtgccg
    17761 ggataaatga tctgaaggat atttacagcg atatcaccga tgttgatgtg gtagttatca
    17821 atcgcttttc cggaataaat tcagagggtc ctactactac tgcgacagaa ctgaatatgc
    17881 ccggcgatgt caccaaaaaa tcctttttcg gcaataaaaa acaatttggc ggaatggcta
    17941 ccccaacggc agtcgttaaa tttagctcat tggataaaaa cgagacctat gattttgtgc
    18001 tttttggtag catgatgaac tgcacggaca acagagaaac ttattacaaa ttttcgggaa
    18061 agatcccggc agataccatc ctttatttga acgcgagcaa taatattaaa aggctggtaa
    18121 aagctgtcgg aatagctccc aacgaaagcg gagaaattga actggaagtc gggccgggac
    18181 ccaacaacga taattcgcgc ggcttttact atatcaccgc cttaagtttc gctccccacg
    18241 atgccgcgtc cggactagac gacccgctgc atttctctgt aaatgcttac cccaatccct
    18301 ttgataaaca cttaaatatc agccccaata ttgatattga gagtattaaa atgatgagtc
    18361 tcgatggaaa ggtaattcat tcggtcaacc gtcttgcttg tggtaaaact catcaaataa
    18421 acacagaaaa aataccatcc ggggtatata tcgtttccat tacgaataat ggggtgaggg
    18481 attatttgaa agtggtgaaa aattgataaa cccctctgcc ccgcacaacg ctcatactgt
    18541 aactatttgt tgcgggttgc atgtttgcga gttccggggc ctcgcggggt aggtgatcac
    18601 gaggtcgaaa ctactcaggg actcgcttga agctatctca aatattcttc cgccagagcc
    18661 acccaatagg ttgcgccgcg gctaagcata tcctgattga aaacatattt ggggtggtgc
    18721 accataaagg tgtcgccgtt gcctatcatg cagtatgtgc ctgctttctc tttcagcata
    18781 aaggcgaagt cttcgctgcc catgtagggg tgcatgtttt ccaccacctg atcctcgccg
    18841 aaggtggctt ttgctacctt tgccgcccac ctggtgtttt cgggagtgtt gattaatact
    18901 gctcctgcta cgccctcgcg aatttcgtaa ccgcaattaa acgcttcagc ttgtgctttg
    18961 gtaattttgc ggattttgtc gagaaccatg atacggaggt cggcctccat attacgtatg
    19021 ctcaaccgca ggatagcttt ctggggaacg gcgttacctg ctatacccga ctggaaagct
    19081 cctacgttta ccaccgagtt tttccatggc gatacgttgc ggcttacgat agtttgcagt
    19141 gccattacca gcgaagctcc gcacaccaac gggtcgatgc ttaactcggg cattgaaccg
    19201 tgtgagcctt tacctgttag ttcaatttcc cagttgtcca cggcggccat ggtttcgcct
    19261 tcgcggaaat gaaattttcc taacggcaag cccggcatat tatgcattcc gtacacggcg
    19321 tcaacaggaa aacgtttaaa cagtccgtct gcgaccattg ccggtgctcc ttccatggtt
    19381 tcttcgccgg gttggaaaat caggcgtacg gttccgttga agtttttggt tgttgctaag
    19441 tattttcctg cgccaagcaa catcgtggcg tgtgagtcgt gtccgcagag gtgcgaccaa
    19501 ccctctattt tgctcttgta cggtaaatcg ttgtcttcta aaataggtag tgcatcaaaa
    19561 tcggcacgca taccaataga tttctttcca ttgcccacgg tcatagatgc cacaattcct
    19621 gtttttccaa tgccttccac cacatcgtag ccccacgatt tcagtctctc ggctatgtac
    19681 ttggcggttt taggcgtgtc catattgagt tctgggtttt ggtgcatgta gtccatccac
    19741 tctttcattt cgggttggag ctttgcgact gcagataata gtttatcgtt cataacattg
    19801 aaattttatg ggtttgtttt cgatcgtaaa ggtataatta ttctcataaa ttgcttgttg
    19861 aaaaatgcga ataaaaaaaa ttaaaaaatt atttttttaa ctacatcgta aaggaaaaac
    19921 catacatttg tcgcgaaaaa aattcaatgt tcaagatagg ttccatgaga gttaaattga
    19981 acaaaatcac agatattcat tcacatcaac aactaaatgt atgaaacgaa caggtcaagt
    20041 actactcatt tttaccctta tctgtacagc ggtttttgct caaactaatg aagagacaga
    20101 caaatcttca acatggaaaa tggtcggcaa tgcccaatat gttactcaac atcattggcg
    20161 tggactcggt agaggtccgc tgtttggcga ggctccggct tttgagccga gtatcagctt
    20221 cttcaataag aactggaatg tgggtgttgt tgccggcggc tctttcgacg gggtttataa
    20281 ggtcgtaatg ccgtggattt cgttttcgcc cgtaaaaaac ttatggattg gcgtttggga
    20341 tatttattct cccggaaaga aattgtggac aaaccaggcc aagccattcg atttcggact
    20401 cacaacttcc aaccactttg tggacgctat gatcttctac caactgccgt ggcttccact
    20461 cacgttgaaa tgggcgacaa tagttacggg aaaagacccg aataccgaag gaaaaagaaa
    20521 tttcaccaca tataccgagc tttcgtacgc gcatcgttgg aatgacttca gcgtttgggg
    20581 tggcctgggt gttacgccct ggaagggact gtacaatgca gataaaggtg gtgtcaacaa
    20641 cttggagctg aagtttcaat acgatttcag gctgtatgaa ccggtaacca tgcccttatt
    20701 tgccaaattc gcatacagcc cgctttcgga acaatttcat tttgtggcgg gtgcaagtat
    20761 aacaattccc tattcattca aataaaacct caaagcaatg caaacaacaa cttacaaagg
    20821 aacagataaa gtacttatag gctttatatt agctgttttg actttttggc tttttgccaa
    20881 ctccatgatg aacgtgtcgc ctgtgatgac cgaaaagctc ggcatggacg cgaatacgat
    20941 gaacatcgcc gtgtcgctcg ctgccctgtt ttcagggatt ttcatagttg tgataggtgg
    21001 attggccgat agcatcggtc gtgtaaaaac gttcaaaatc gggttatatt tagctatagc
    21061 aggctcgctt ttagtgggac tatcacccaa aggtagcctt gctgtgccca tgcttcttac
    21121 agggcgcgct ttgcaaggtc tttcggcggc cttcgtaatg ccgaccagct tgggtatgat
    21181 taaaattttc tgggagggca aggagcgtca acgcgctatc agtctttggt ctatcggaac
    21241 atttggtgga tcgggattaa gctcacttac aggaggaatt attgcatcga gcctgggttg
    21301 gagatggatt ttcttttttg cagtaatagt ggcaatcata gctttatatt tgacaaaaga
    21361 acttcctgaa aataaacgcg aaggagcagg caaatacaaa attgactggg gtggaataat
    21421 cgtgtttatg attgccatga tcgccttgca gattttcatt accaacggaa acaaattggg
    21481 ctggacaaac tggataacca ttgttttagt tgccattaca atagtatttg gctacttgtt
    21541 tgttcgcgtt gaaaaaagat caccctatgc ttttgtagat tttggtttgt ttaaaaacaa
    21601 aatatttacg ggagctacct tggcaaactt cttcatcaac ggaacttcag gtatgcttat
    21661 cgtttctctt acattgatgc agcgtggggc acaattctcg gctatggaag ccgggttgct
    21721 gacattaggt tttgctattg tcgttatctt gtttattcgc gtgggtgaaa agctacttca
    21781 aagatttggt gcaaaaaaac ctattatctg gggagcacta ataatttgtg tagccattat
    21841 actgcttatg cagacacaga taatgacagg gcaatatgtt gtgttggcag ttattgctta
    21901 ctcgcttttc ggattgggtt tggctttctt tgccactccg gctaccgatg ccgctttatc
    21961 caacttgccc gacagtcagg caggtagtgg agcagggatt ttcaaaatgg catcatcgct
    22021 tggaacaagc ttcggagttg ccattgcagc aggaatttac acggcagtag tctccagaac
    22081 agagccgata ggctggttga gcaacatcat aggcttcgta ggcagacaag ataatgtggt
    22141 cattcgacaa ggagcaatgc tcggtttggc tttctgtctg cttttggcga ttttggtgat
    22201 tgtatcggtg tcaacaagca taccggataa gaaaaagaca gacaatgtaa aataattttt
    22261 gcatatcatg actacaaaat cagtacagta caaaggaaat gacaaacttt tgtttggtat
    22321 tattctgggt gtactggcat tctggctttt tgcccaaact acgctaaata tcaatgtaga
    22381 tatggcgaaa gatttgggca tggaatcctc gatgatgaat attgccgtgt cgataacatc
    22441 gttgttttcc ggcattttca tcgtggtgtt cggtggcctg gccgataaaa tcggtcgagt
    22501 aaagatggta aagtacgggt tttatttcag tattatcggt tctttgctgg ttgggtttac
    22561 tcctgtgggc ggtgcatccg gcgtagtgtt gattttagga cgtatttttc agggactttc
    22621 cggagcggct attatgccct cgagtttggc attggtaaaa gcttattggg agggagccga
    22681 acgtcagcgc gccattagtc tctggtccat gggttcgtgg ggaggttccg gatttgctgc
    22741 acttttcggc ggattgatgg taggcatcgg ctggcgttat attttctttg gagcagcagc
    22801 tatatcgctt ataggtttgt tgatgataaa agatacaccc gaaagcaaag ccgaagttac
    22861 agacaataaa aaaaccgact ttgctggaat cattaccttt atgttatcga tgatcggctt
    22921 gcaacttttg gtaactaaag gttcggagtt tggttggaca agtccgttgg gacttactct
    22981 tattctgttg acagtggcat ttgcactcct gttcttcaaa attgaaaaag ggaaagatgg
    23041 agcctttatc gattttaaac tattcaataa cttaactttc accggtgcca ccatatccaa
    23101 ctttatgata aacggaaccg caggcatgtt gattgtttcg cttgcattgt tgcaagtggg
    23161 aggaaacctt acggcaaaag aagcaggact tcttacgctc ggatatgcca tctctattgt
    23221 gttgtttatt cgtgtgggag agaagttatt acaacgtttc ggagcccgca aaccgatgat
    23281 ttggggttcg cttattgtgg gtttagccat cattctgttg atgtttacca atgtgatgac
    23341 cggcacgtac aagattttag ccattatatc gtacacactt ttcggtttgg gattggcttt
    23401 ttacgccaca ccatctaccg atgcagcact ctccaacttg cctgaatcgc aggcaggctc
    23461 aggctcgggg atttataaaa tggcatcgtc gcttggggcg gcatttggtg tggctatttc
    23521 cgccggtgtt ttcgcgacac taaacgtagg agacaacatc acctggatgg agggtgtgct
    23581 tactttcgtg ggccgacaag acaacgttgt agttcgtcaa gcggccacca ttgccttagg
    23641 tgtaaacctg atgatggttg tgatggctat tgtaagtatt atggttacta ttcccaagtc
    23701 gggtggaaaa cttaagaaat aagtaaagct atctaacgat gattttgtgc cttggtggtg
    23761 gaatactgcc aaggcacgat ttttgtttgt tcgccggccc gaacattctc taagcaacag
    23821 ttaaatataa taggaataga tttttttaac attgaaacta ttgtttttaa aaatatatcg
    23881 tctacctttg tatcggcttc cgatataacg agtggcaata tagtctatag agacaatgaa
    23941 taaaagaaca agtaccgtta aagaactgag aaacaggctg gcaataggac tatagagaca
    24001 atgaacaaaa aacaggaacc gttaaaggac tgagaaacag agtggcaata gaacctatat
    24061 agacaatgaa caatgaaaca aatattgtat taactgaagc agtattttat attctgctcg
    24121 tcctgcataa acctctccat ggctatggaa ttatgcagga aactgaaatt atgagcggag
    24181 gcaggttaaa actttccgca gggactcttt acggtgctct gtcgtcttta ctcgaaaaaa
    24241 aatggattat cgagttgccg atggaagagg gcagtcgtag aaaagaatat gagattactt
    24301 cgctcggcaa agaagtggca aagatagagc taagccgctt ggaagaattg gtaagaaatg
    24361 gtaatagtat catcgaacga taattatcgg tgaaacagct atttagagat aattttcgat
    24421 tataaattga cgacagattc tatgtgtttt taaaaaataa aaggcatgaa aaagaaagtt
    24481 tacaaactct tctttgtttg ggaatacgac aaagaagaga aatggctcaa taagatgtcc
    24541 gcagagggct ggcaactgat aaaaggcagt tcttacagct acctttttga aaggggagag
    24601 cctaatgaat atgagtataa attagaattg ttagacgaag agcccgactc tctcaaaagc
    24661 aaaaactaca ttgacttcct aaaagaagct aatattgaga atgtagggaa atgcagaaat
    24721 tggatttttc tgcgaaagaa gacggctgac ggtgggtttt catcggataa taaaacttta
    24781 agtcacatca atcattcttt gcgtgtagag gaaatttatc acacaagcag aaatacattt
    24841 atcttgttat tgtcgattgc gtttataggg tcttttgtta caggttattt accggaatct
    24901 gccatagtga gtttttttag cggttttttc atgggaattg gtatatcggc tgctgttctt
    24961 tctttaatct taatgccgat tatgcgagct aattttaaaa aaataaaaaa ccacattcgc
    25021 gaattatata tagtcgaagg ggtataaaaa gaatctgtga agacgatcgg tatagaaggc
    25081 aatcaaagcg ctggatgagt gtataaaaag gtaaatataa ttagccaaac agattgtaaa
    25141 acaaataatt aatttaaaaa gtgaaaatca agagaatggg cttattggcc acaataattt
    25201 taacaactat gatggtaaat gcacagccgt ttgccggtga ttggaaagga gctatttctg
    25261 ttcagggaat ggagttggaa cttattttcc acatcgcgga agataatggc acttatgcta
    25321 caacactgga tattccgatg caaggtgcag caggaattcc ggtagaaaag accgaagtag
    25381 agggtagtaa attaaccctc aatatgtcag ctttacaggc taggtatgaa ggtactctga
    25441 acgttgagag tattgaagga accttcaagc agatgggaca ggagttttcg ttggttttga
    25501 aaaagttcga aagtaaactg cccggaaatc ccgcactggt gtcgtccgat gaagaattgc
    25561 agcaacttgt agcttatgac gcaggcaatt acaagtatag ggtggaagac tatttttcac
    25621 gtccgaaagc gagttcattt caactctcac ccgatggtaa atatatgtct tatcgtgaaa
    25681 aggaagatgg aagtaccaaa cgtcatgtgt acgttaagga gattgccacc ggaaaagtta
    25741 cccgtatttt ggaagaaaaa gacgagttga ttcgcggtta tttttggaaa aacaacgagc
    25801 gacttcttta tatgatggac caagggggaa acgaacaata ccacattcat gccgttaatg
    25861 tcgatggttc aaacaacgtg gacttgacgc cctatgaagg tgttcaagcc ggtgttgtca
    25921 gtattttgaa ggatcaaaaa gattacgtta ttgttcaaat gaacaaaaac aacaagcagg
    25981 tttttgagcc ttataaactg aatgtcgtta ccggagaaat ggagcaactt tacgagaata
    26041 aagatatcgc caatccaatt caaggctacg attttgataa agacggcaac ttgcgcggat
    26101 atagccgcct ggtaaacggt gtagaatctg aacagtggta taaagatctg gcgaccgggg
    26161 aatttaagct tgtaaaacag atgaaatggt acgattcttt cggtatcgca ggctttaatt
    26221 atgacggtaa gaaccctgac gaggcttatg tggttaccaa tctcgattcg gacaaagcgc
    26281 gtattgtgct gtatgacttc aagaaaaact ctattgtaaa agaagttttc tccaatcctg
    26341 attacgatgt agccaacatg agccgcagtc gtaagcggaa ttatgaaatc gactattttg
    26401 cttacaacgg cgaaaagtat gttatcacgc cggtcagcga tttctttaag agtttcacca
    26461 aacaaatgga gaaagaattt cccgggaaag agttctatgt tgttgattcg gatgacgatg
    26521 aaaacacgtt cctgatagtt gtccaaagcg ataagctgta cggtacgtac tatcaatacg
    26581 ataataaaac caaacaattc tcgctgcttt atgacttaat gccacagcta aaagaagctg
    26641 atatggcaga aatgcgtccg attacattca caagccgaga tggactcact attcacggct
    26701 acatcacatt gcctaaagaa gctttgacag gtgaaaaagt gcccttggta gtaaacccgc
    26761 acgggggtcc gcaaggaatt cgtgacgaat ggggcttcaa tcccgaaaca caactttttg
    26821 caagtcgagg ctatgctacg cttcaagtaa acttccgtat atccggcggt tatggaaaag
    26881 agttcctgaa agcaggtttc aagcaagtgg gaagaaaagt gatggacgat gtggaagatg
    26941 gtgtaaaata cgtgattgag cagggctggg tagataagga caaaatggcc atctatggcg
    27001 gtagccacgg aggatatgcc acattgatgg gattggtgaa gacacccgat ctctatgctt
    27061 gtggggtaga ttacgtgggg gtttcgaata ttttcacctt tatgaattcc attcccgaat
    27121 attggaaacc ctatttggat atgctaaaag aaatctggta cgatattgat aatgaagaag
    27181 aagctgccat tgcaagagaa gcttcgccgg cgtatcagat tgataaaata aaatcgccgc
    27241 tcttcgtagt gcaaggagct aatgacccac gcgtaaacat caacgaaagt gaccaaatag
    27301 tggagaaact tcgtgccaaa ggagtagatg tgccctatat ggtgaagtat aacgaaggac
    27361 acggttttgc acacgaagaa aacagcattg atttctacaa agccatgctt ggatttttgg
    27421 cgaaatattt gaagtaatat aaatcgcaga ccgatgtagg acgaagtgga ttaatacaaa
    27481 ccgggagtat cagttatatc gacgctcccg gtttttattt tgatattcta tagaaaaaag
    27541 tactaccttt acgactgtga gcaaaccagt acaatcaatt ataattgagc taattagatg
    27601 agataaggaa cccaacaaga aggggagcag aacaattatt gaaaaagatt gagaaataac
    27661 gaatgaaacg aagtaaaata ttcggacttc agccaaacgc cttctttacg gggttgacaa
    27721 gtttttttac cgacacatca gctaaaatgg tatatagcgt tatgcccctt tttttactat
    27781 caataggagc atctaaaaca accatttcct tgattgaggg aattgcagaa agcacagctt
    27841 ctttattaaa ggctatttca ggctattgga gtgataagat tggtaaaaac aagccattca
    27901 tgattattgg atatggtatt a
//
LOCUS       DAOSGL010000002        25616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_1514_length_25616_cov_4.818513, whole genome shotgun sequence.
ACCESSION   DAOSGL010000002 DAOSGL010000000
VERSION     DAOSGL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_25616_cov_4.818513"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKY68_00090"
     CDS             complement(<1..626)
                     /locus_tag="PKY68_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOH23473.1"
                     /translation="MNKTKKNKLRFIILSAALVCCLGVFYAFKAQDRNFLISKNFDIF
                     HSLFRDLNLFYVDSVDVDQAFSNAIDGLLSSYDPYTSYIPESQMSELKYMTTGEYGGI
                     GAVISKRGEAIIVAEPYENMPAQEAGLLFGDELVEIDGISMEGKTVSFASDCLKGQPN
                     TSVELLIRREGEKKLIKKTLQRKLIQMNPVEYACLLKPGVGYVYLSSFT"
     gene            complement(708..1685)
                     /locus_tag="PKY68_00095"
     CDS             complement(708..1685)
                     /locus_tag="PKY68_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOH23474.1"
                     /translation="MKNLLALGNALVDVLIRLDSEEILTELGIAKGSMQLIDKERFDL
                     IQQSLKPYKKQYASGGSAANTIIGTATLGLPSAFIGKIANDGNGAYFEKDMKRAGVET
                     RLLKGQSSSGTAFTFVSPDGERSFATYLGAAAELQAEDLTPQMFKGYANIYIEGYLLQ
                     NYDLILRIMELAKAAEMNIIWDLASYNIVEEHKTFLTEKVLPQVDYLFSNEMEAEALT
                     GAKADEALDLLAAGTKVAVVKMGAKGAMAKRGQEIARVPALKVNALDTTGAGDLFAAG
                     FLYGLFIGLPLNEAASIGNITAGNVVEVLGAKMDQTRWAAIKKQIKSLL"
     gene            1770..1843
                     /locus_tag="PKY68_00100"
     tRNA            1770..1843
                     /locus_tag="PKY68_00100"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1804..1806,aa:Asn,seq:gtt)
     gene            1936..2472
                     /locus_tag="PKY68_00105"
     CDS             1936..2472
                     /locus_tag="PKY68_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOH23475.1"
                     /translation="MKQHPLNLFSFCPVCGSGRFVENNFKSKRCMDCGFVYYLNIAAA
                     VAAFILNPAGELLLCRRAKEPALGSLDLPGGFVDMGENIEEALRREIYEELSLKVDTT
                     RYLFSLPNIYKYSGMEIHTLDFFFLCLVSEFDSIQANDDVGEAFFRPLNKIYPADFGL
                     DSIKKGIIAFLNETPQIR"
     gene            2476..5514
                     /locus_tag="PKY68_00110"
     CDS             2476..5514
                     /locus_tag="PKY68_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH23476.1"
                     /translation="MLSYIKRKSLIFLMLMAFSAGSFVSAQENSQKKGPTWLYRLASE
                     QFNAGEYAYCLRTLDTWFEQEQVLPENLRENARFMYAASSYELNRMESSLLLKEFIGE
                     FPLSVQASRAYYMLGINAMNARQYQDALAFFLSCRSKDFSKKEEAGYRFYLAFTYLQL
                     DELDQARALFEGLLQEDQRYVSASTYYLAFIDYRQGRTEQALEGFGKIAAHPEYERIV
                     PFYIMQLYFDQGKNKEMLEIAEKLLQEDPNESERIELYRLSAAGYYELNDHALSQEYY
                     GKYLRYNPELLRSDAYRIGINHYVAKEYEQAAKYFLQVSQEGDALAQNAEYHLGLCYL
                     QQQKFDQARMSFEQASLSDQDKEVKESALYNYALLCYQTSFSPFNEQVRAFERILTEF
                     PNSRYADRVYGHLVDAFLSTKNYQASFDVLNKIADPGKDLLETKSKLLFLMGMDRFNN
                     AAYGEALTYFNSSIQLSTQLRLEAPETYFWRGETHYRLENLRAASNDYLRFLNSPNAS
                     NYKAWQLAHYNLGYSYYNLKQYPEALNWFLKYSQFKEIRNDNTYPDVLNRIGDCYFHA
                     RNYDRAEEYYNMSDKLSETGNDYAVFQRGFSMGLQKRHEEKARLFAGFEKRFPLSDYL
                     DDALYEQGRAYIALNRPAEALPVFEKLMQKFPNNILARKAGIQIGLLEYNRNNTEAAI
                     AAYKKVINEYPGSQEAQTALNDLKMIYVAVNRVPEYLNYVDNLGSGVRIERSEKDSLS
                     YLAAERLMVDGKNQEAIKGFQDYINAYPQGMFRTEAYFHAGNLLLLSNETSRALPYFQ
                     EVIKQTGHRFVPEALVVLAEHEYKQKEYARALEHYQSLDVLAEDRSVRIGTRSGILRC
                     LYALGRNESIPAAADKLLAEENLSPSLQREALFRKTIALMELNRTDEALSDLQRLAEE
                     SQTAFGAEARFRLAEYWFNKNDLKKAEEIAQSFLRDGTSQSYWLARCFVLLSDIYIQR
                     KDYFQARQYLMSLKENYRAEDEIAGMIQTRLDNIAQLSKE"
     gene            5517..7334
                     /locus_tag="PKY68_00115"
     CDS             5517..7334
                     /locus_tag="PKY68_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23477.1"
                     /translation="MKKNTLCFLLVLSGLFTMSAVAQTDRDSLIRRDLLLEKEYNPML
                     ETAGKIYRVPETEELKTKGTEVRFSLSEAPTLTPGEYVPLRAAEARTYFPAREQKAYV
                     RLGAGYDWSGLGDLQWNILKGSRHLLDFTAWHRSSFGKVTLQPDLGTRAKHIDNRLGL
                     NYQWHLSSAEFRAGLSERFYAWNYYGRSWSNLPLQNRDSLVVPDLQWFSDTKLSLGIE
                     SKPWDKAFDYAFNVDAHYFRLGKALAKDSLAGAGPAETEIDAGLALNYQFNDNLDIGL
                     DARFRAFFYQRGLSSQRFSLADHMQYDPLFPAYNDAPANVLWFEMNPHATYSLGLWDF
                     GLGFRLSYLDGQKLLIAPTASVDRPLGNWAKLNVGIDGGQRLYSYREGLELNSYLDPS
                     IRLLPEYSPVALRGALQVKPFSGLNMAVFTGYSFTRDLAMFYNALPVDSGLLNHLSYS
                     SLFMADYVKMNKLYLGLDFNYVYRKTLNLSGKISYNHYIASSDNAKTDILLESNARRP
                     WHKPAFEGEALLSLTPGEQTAFFASYHWEGVRYAPDYFNTYRSLPDIHYLSLGASYDI
                     TRRLGVFLHLNNVLNQFYEVWNAYPSQGFTAVVGVSYSF"
     gene            7341..8060
                     /gene="recO"
                     /locus_tag="PKY68_00120"
     CDS             7341..8060
                     /gene="recO"
                     /locus_tag="PKY68_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOH23478.1"
                     /translation="MLVRSQALVLHQIPYSDAFSVVHLYTREFGRQAYLLTRSRKNSK
                     LRQSLFQPLSLIEYEADHKGSRELQRLKEARSLFAFTGIPMDPVKNAIALFLSEVLYR
                     ALEERESNPALFDFLVQSVEILDLCREGAANFHLVFLIKLTRYLGFYPNLEDKAPGKY
                     FDLQAGTFCSLQPSHRDWLSPEDAQSFAVLLRINFENMPAFRYDRYQRQRILRQTLDY
                     YRLHLSEFPRIKSLDVLQELF"
     gene            8173..8244
                     /locus_tag="PKY68_00125"
     tRNA            8173..8244
                     /locus_tag="PKY68_00125"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8206..8208,aa:Arg,seq:cct)
     gene            8743..9312
                     /locus_tag="PKY68_00130"
     CDS             8743..9312
                     /locus_tag="PKY68_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23479.1"
                     /translation="MSSGSRFPLVVSVVSSVLDYLFPFLGEREVGEIQQTKRPKPDYR
                     RQKRALILKYGNPTYNLVLKEQNLNEEIIIFGKTRRIMIQGKLFSHSDILSCTYTDSQ
                     RILKGEATFVGKLDTCKMPSFRGFEGDTNLAAYFEGNNVSNKEIITHNYTVIIKLRDL
                     SASVLKIPMGSNWEKVNELLEAIKFFISS"
     gene            9459..9947
                     /locus_tag="PKY68_00135"
     CDS             9459..9947
                     /locus_tag="PKY68_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOH23480.1"
                     /translation="MNKRNFPLIGLAVFFLLLPCTVRAEKKKQIELMAVTKVVDGDTF
                     WVDDGSEKGKKVRLIGVDTPETLHPQKAPEPYGREASEFTKSLLEGKKVRLEFDVDSL
                     DRYGRLLAYVYLENGLFVNAELVRKGYAQVATFPPNVKYADYFLKLQRKARNRKLGLW
                     RE"
     gene            complement(10029..10739)
                     /locus_tag="PKY68_00140"
     CDS             complement(10029..10739)
                     /locus_tag="PKY68_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH23481.1"
                     /translation="MKTAIVSGGAQGIGKAIALKLKKEGCAVVVLDSNTEQLKALEAE
                     NSGIFGLLCDVANPDEVNDAMAKIEALYGKVDYLVNNAGISYFEPMDSMSIDTWNRIL
                     AVNLSSVFFLSKACLPLFSENSAILNIASTRALMSEPGGEAYGASKGGIVALTHAMAN
                     SLGPKTRVNCISPGWIETKHYEKLSETDHKQHPAGRVGKVTDIAEAAFFLLSESSGFT
                     TGQNLVIDGGMTKKMIYE"
     gene            11016..13268
                     /locus_tag="PKY68_00145"
     CDS             11016..13268
                     /locus_tag="PKY68_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH23482.1"
                     /translation="MLRTFFVWLTMTGIVVAMSAQETNAENPSERQQTHFEWTVQGNP
                     IVTHKYTADAAVMVREDTLWLFTGEDAAGNQNRYIMHNWCVFSTTDMKHFKEHPIPLY
                     GADFKWSSRQAYAGHVTERNGKFYFYVSTNATGIGVAIADRPEGPYKDALGKPLLTNA
                     DCFASTHSWACIDPAVFIDDDGQAWIFWGNRECYYAKLKENMIEIDGPVKQIMFDGFS
                     FTEAPWIHKKDGKYYLTYASGFPEKIHYAMSESIEGPWEFKGILNEIAGNSNTNHQAI
                     VEYKGRWYFFYHNGAMQTQGTSYSRSVCVDYLYYNEDGSLKRVQMTSEGVDHIPGEKD
                     AAFDTLAVNGAWCWFADPRAIYHKGEKEQTYFSWVTTSGDIVVASYNHQNGEFIQHVV
                     LEKFQSDDHANPTLFIRDDGRIIVFVAAHFGNTIKRCITTNPEDISSWGEVYEFGHTV
                     TYPYPFQLGKDSILVFFRGGSSWKPHMILSTDNGETFGTQKEFIVSTGKRPYMRYCQG
                     ADGSIHMAATTGHPRDVLDNKIFYCCFKNNRFYRADGSLIKDFGREPLDVSEMDEVYE
                     GISCGKGWIWDIALEAETGFPVMVYASFPDDTDHRYHYARWNGKNWTSTELAKAGKWF
                     PQTPKGKIEPEPNYSGGIILDYDDPSIVYLSKQVDGVFELFKYTTPDHGQSWESEALT
                     ENTPKGLLNVRPIVPRHHKKGVFDVIWMRGRYEFYADERYNTSLVFPKMEKTGELEER
                     DFNTSGSPAI"
     gene            complement(13297..14466)
                     /locus_tag="PKY68_00150"
     CDS             complement(13297..14466)
                     /locus_tag="PKY68_00150"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOH23483.1"
                     /translation="MTTVNLMYNVGSKDEHPDHTGFAHLFEHLMFAGSARVPDFDKAI
                     REASGENNAWTSRDVTNYYITLPSHKLDTALFLESDRMSSLNINQRSLEIQKQVVLEE
                     FKQRHLNQPYGDVSMLLRSLAYEGHPYSWPTIGKSTEHIEAFNLDDVHAFYRRHYNPD
                     NAVLAITGDFDFEHLAKLSEQYFGAIASAETDKPEFPAWPQQEGPRFLEVERPVPGPA
                     LYKLWRAYAVCEEEYITCDLLTDALSLGNSSRLYQQLVKERQVFSQADIYVGGEGLAG
                     AGSILFSGKPAKGVSLEEAEKALEACLNQIATEAPGQEELNKILNKHESAFLFQNTDA
                     RHLATQACWYEILGSADNYLLEPERLKKLSGEDLRSMAENVFRPENCSTLYYQNA"
     gene            complement(14538..15362)
                     /locus_tag="PKY68_00155"
     CDS             complement(14538..15362)
                     /locus_tag="PKY68_00155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH23484.1"
                     /translation="MKKLFFFISVLLLTSQTHMMHSEEKATFVLMETSMGKVKIKLYN
                     ETPKHRDNFIKLVNEGYFNNLLFHRVIKDFMIQGGDPESRNAPAGKQLGSGGPGYTIP
                     AEFVYPKYFHKRGALSAARTGDQVNPQKASSGSQFYIVWGQVYKASDIDQLEAQKINQ
                     AKNAYFQQLAGKQQSKIQQLYQVGDQAGLQKLQNELIAQMEAYFEANPPQGLSPEQIK
                     AYTSLGGTPHLDNEYTVFGEVVEGLEVVGKIQSVETGTADRPKTDVVILSMQVIGN"
     gene            15439..16347
                     /locus_tag="PKY68_00160"
     CDS             15439..16347
                     /locus_tag="PKY68_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOH23485.1"
                     /translation="MTKPTLFVLAAGMGSRYGGLKQLDGVGPRGETIMDYSIFDAARA
                     GFGKVVFVIRKSFEEDFKTKIVSKYQGLMPVEIVFQDLNDLPAGFVCPPGRQKPWGTN
                     HAVLMGKDVIKEPFAVINADDFYGKESYVVLAKALNEMQGKKNHYCMVGYRIGNTLSE
                     SGSVARGICQTDENNCLTTVVERTWIIRDQDGLVKYKDENEQLVTVGENTPVSMNMWG
                     FTPDYFDYSETNFIEFLKENIDNPKAEFYIPWVVNNLVNQGKAVCKVLDTPSKWFGVT
                     YAEDRPTVVSKIKELVEAGEYPSKLW"
     gene            16394..17536
                     /gene="galK"
                     /locus_tag="PKY68_00165"
     CDS             16394..17536
                     /gene="galK"
                     /locus_tag="PKY68_00165"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOH23486.1"
                     /translation="MDYTAISTQFKKRYGSKFSLYASPGRVNLIGEHTDYNGGFVFPG
                     AIDKLIVAAINPNGTDMVRVYSYDMKETAEFGLDERPIASWALYIYGVIREIVKRGLR
                     VGGFDCVFGGDIPIGSGLSTSAALESTFAFALNDLYKLGLTKLDMALIGQAAEHNAVG
                     VRCGIMDQFASVFGETDHLIRLDCRSLEYELVPFHPKNCRLVLLDTQVKHNLAASEYN
                     IRRAECEEGVSIIQKKHPQVTLLRDVSLDLLNDFKKELRESTYRRCRYVIEENQRLLD
                     ACEALKHDDYAEFGKKMYGSHEGLSKEYNVSCEELDFLVSIAVKNGALGARMMGGGFG
                     GCTINLLEDSHYSDFIEEAIAAYKKQFNRAPKVYDVKIGRGSHKIA"
     gene            17539..19011
                     /locus_tag="PKY68_00170"
     CDS             17539..19011
                     /locus_tag="PKY68_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOH23487.1"
                     /translation="MYLLGYDIGSSSVKASLVGAQSGECIYSAFSPVTEMSIKALQAG
                     WAEQDPEDWWQNLKIVTKEILLNTRVEATSIKAIGISYQMHGLVLVDKQQKVLRPAII
                     WCDSRAVPYGEKAFRDLGEERCLSHLLNSPGNFTAAKLAWVKENEPELFKQIDKILLP
                     GDYIALRLTGETSTTASGLSEGIFWDFKLHQPADFILDYWGIPREWIPVILPSFSEQG
                     RLSASAAGELGLSKGTSICYRAGDQPNNALSLNVLNPGEIAATAGTSGVVYGVNDSLA
                     YDPKSRVNSFAHVNHSKESIRIGVLLCINGTGILNAWLKRNSASNSLSYAQLNELAAT
                     APIGADGLSILPFGNGSERMLENKASSCSIHGLQFNRHSQAHLLRAAQEGIVFSFAYG
                     MEIMQAMGINTALIRAGHANMFLSPLFRESLSTLTGAAIELYDTDGSVGAARGAGIGS
                     GVYLTPQEAFGKLKRIARINPKPAEQPAYLEAYDRWKQYI"
     gene            19037..20350
                     /gene="xylA"
                     /locus_tag="PKY68_00175"
     CDS             19037..20350
                     /gene="xylA"
                     /locus_tag="PKY68_00175"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOH23488.1"
                     /translation="MSTKEFFPGIGKIKYEGPQSKNPLAFRYYDANKVVMGKTLAEWT
                     RFSMAYWHTLCAEGGDQFGGGTKTFPWNKGTVLERAKAKMDAGFEFMSKIGIQYYCFH
                     DVDLVDEAETIEEYEANLKTMVEYAKKKQAETGIKLLWGTANVFGHKRYMNGAATNPE
                     FASVAYAAVQIKNALDATIALGGENYVFWGGREGYMSLLNTDMKREKAHLAMMLTKAR
                     DYARAKGFKGTFLIEPKPMEPTKHQYDADTETVIGFLRAHGLDKDFKVNIEVNHATLA
                     GHTFEHELQCAADAGMLGSIDANRGDYQNGWDTDQFPMNLNELTEAWLVILQAGGLGT
                     GGTNFDAKTRRNSTDLEDIFIAHIAGMDAFARALLAAADILEKSPYKKMRKERYASFD
                     QGEGKAFEEGRLGLEELRAFALKNGEPRQISGKQELYEAIINMYL"
     gene            complement(20434..>22272)
                     /locus_tag="PKY68_00180"
     CDS             complement(20434..>22272)
                     /locus_tag="PKY68_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23489.1"
                     /translation="RKGTTAEYDSVWPNFSVVIANNIFYKIGGNATANKHFLIYNGAL
                     GGLNAKVKINNNLFYKRWSEQHAPIAYISLPAALATDTQLIEVEVLNNFFYPNNVVSV
                     VDGQALTLSEASAVTPSYNLLTASGLGITDVFIDEEALTISKGSPLFISGTGRTPVGI
                     VERYMDRTESGIIRVSNSFEFRTALSIAIGGDVIEVENNLTDTLGIYQIGQGMAFPQT
                     FGNLTIRAAEGHSPTIFGRLSSNNGTKLNTFLVEGLHWFGGDSGIVNFNSDSYAPFYI
                     STADSIMDAFIVRNCTFTNLDYQPIMRTNNCANALINEVLFEYCTFDNMGWNKASGNQ
                     GYHFFQFLSNPTYELDKFTFRENIVKNFHGSQFFNITREGSPSHKGDSTIYITIENNT
                     FYKIGGTGASARQFLGFNKKPAGFDAYIKINNNLFYKRWSNQNHPISNLALYYPDTDQ
                     VLDVQVLKNFFAPDSVCAQKGKTVSLPVSEGDETKVTFERLFTSTLFLDSVFYNEDKL
                     QIDGSTSPLYTAGLNGTYVGAKATYINVDGISKPSVSGKDRNVFTKNGVLYIQTDSKQ
                     TVEIYNVLGKLIHCVQVNEGVNAIDGLNTRQIYLVKTNNDVVKVIL"
     assembly_gap    22273..22282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22283..25078)
                     /locus_tag="PKY68_00185"
     CDS             complement(<22283..25078)
                     /locus_tag="PKY68_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23490.1"
                     /translation="MKKILTPLLMTLLLFLVAVPKVDAIITDPNDYIIYDSGIMADSV
                     HCSTWEFPSFMTITDNVSVKKVNKWGRHNNSVVDYGYPPKTDVATLKGKVDLWGIVIG
                     QKSGYSGISYTAFLEQPEEERPLFYVNRWKGGRLYGRPTDSIVIRIPTTSDSIFIQGC
                     GTDSFYAIMAYELHRGKDSAWYIGGNWGNVFNLGFKPKHAPGTDSTDIVVLGPHKDWY
                     TSGVEWQSNAFQMRLVNAADQNEDGTMKNKKSERWGFFEGTSINRIKIFGQIEKGSVP
                     EFDGTIAGWDFLYRPKASGTVDLTSFVTDIDGEWEADYYSLYASDLGVVRGMLTASSP
                     SDGMQLGKDCGVKAVMISPAGKNDTIDFTLAEEHANYFQIDVSLQDFQEINLNFDFNL
                     RDAADSCVVAYAVDGADTFTVAGKFAAPEDWDGLTHVSVDLPELTNQSYVTIRILQNN
                     GALDENGQFILANLSINGFDYVEHKEGALNVAYINTIADRIKTFDRAITSDSLDKVIL
                     PALMENFNVTYVYPDIYAGISDSAGVNALFADYDVVVLSEFPDADSDIAKACKYLVGN
                     KPLLNLKAMTYNSNSWNWGVAGNGKYDQATPVELRAKVQSNMVFHPVFKNIELDSLKA
                     FNVFDTLFLSRGATSNDFKGLQGFSSYKGPQAYTLAVMEQSPDLACIQEINSNPAAKY
                     MMIGLSAENYENVSEEGVQLIINAVNYIGSGDVFAVPNFNMTSSGATVENTEELIAAL
                     AYDYSALNLDIVTIKLKNSTDASGVYEIGNSGMIFPRSSNDLVLQPASGATPSIWGRF
                     YATNGMKLNNLTFKNLTWDGGNTALANYDNASYEPFYFNKADTLTGGITVENCTFKNF
                     NSTSVMKTDNCAGALINAVVFRGNLFENIGGSKASGVDGPTLIEFSDAANYQCDSLLF
                     SKNIVKNFHGNHLFNIPR"
BASE COUNT         6710 a         5652 c         6202 g         7042 t
ORIGIN      
        1 gtgaagctgc tcagatatac gtagcctaca cccggtttca gtaagcaggc gtattctacg
       61 ggattcattt gtatgagttt acgttgcagc gtttttttta tcagcttctt ttcgccctcg
      121 cgtcttatca gcaattctac gctcgtgttg ggctggcctt tgaggcagtc gctggcaaag
      181 gatacggttt tgccttccat gctgataccg tcgatctcga ccaactcatc tccaaaaagc
      241 agtccggctt cctgagcggg catgttttcg taaggttccg caacgatgat ggcttcaccc
      301 cttttgctga tgacggctcc tatgcctccg tattcgccgg tggtcatgta tttcagttcc
      361 gacatttgcg attcggggat gtagctggtg tagggatcgt aggaactcaa cagcccgtct
      421 atggcgttgg aaaacgcttg atccacatcg acactgtcta cgtagaacaa attgagatct
      481 ctgaagaggg agtggaaaat gtcgaaattt ttactgatca aaaaatttct atcctgagcc
      541 ttaaaagcat aaaaaactcc caggcagcac accagcgcag cagacaggat tataaagcgg
      601 agtttatttt tcttggtttt gttcatcggt ttttcttgga tatatgcttt tttttatccg
      661 gatgcaaggc cgccggaaac ttgttttcgc cgcgatctta tccggagtca aagtaaggat
      721 ttgatttgtt ttttgatggc ggcccatctg gtttggtcca ttttggctcc cagaacttct
      781 accacattgc cggcggtgat gttgccgatg ctggcggctt cgttcagggg caggccgatg
      841 aacaggccgt ataaaaagcc ggctgcgaat aaatcgccgg ctccggtagt gtcgagagcg
      901 tttactttga gggccgggac tcgggctatt tcctgacctc ttttggccat ggctcctttg
      961 gctcccattt tgacgacggc aactttggtt ccggccgcca acaaatcgag ggcttcgtcg
     1021 gctttggcgc cggtaagcgc ttcagcttcc atttcgttcg aaaacaggta atctacctga
     1081 ggaagtacct tttcagttaa aaaagtcttg tgttcttcta caatattgta actggccaga
     1141 tcccagatga tgttcatttc ggctgctttg gccagttcca tgatacggag tatcaaatcg
     1201 taattttgca gtaaataacc ctctatatat atattggcat aacctttgaa catctgtggt
     1261 gttaaatctt cggcctgcaa ttcggcggca gctcccaggt aggtggcaaa actgcgttct
     1321 ccgtcggggc tgacaaaagt aaaagccgtt cccgaggagg attgtccttt gagcagacgg
     1381 gtttccacac ctgcccgctt catgtctttt tcgaaatagg ctccgttgcc gtcgttggca
     1441 atcttgccta taaaagctga aggcaagccc aaagtggcgg tgccgatgat ggtattggcg
     1501 gcggatcccc ccgaagcata ttgtttttta taaggcttga gcgattgttg aatcagatcg
     1561 aaacgttcct tgtcgatcaa ttgcatgcta cctttagcta taccgagttc tgtaaggatt
     1621 tcttccgaat cgaggcggat taacacatcc accagagcgt tgccgagggc taataaattc
     1681 ttcattctgt aaattttttg gcaaagatat tgcaatattc gacaatatgc cgtatttttg
     1741 tcgcgctttt ttaaatgtaa gctaaacatt cttcagtagc tcagtcggtt agagcatctg
     1801 actgttaatc agagggtcgt aggttcaagt cctacctgaa gagctaaaga gtccgaaccg
     1861 tccataccgt tcgggctttt tttctaataa ccggcagctt ctaaataacc ggctgctatg
     1921 aaataaccgg ctgctatgaa acaacatccc ctcaatcttt tttcgttttg tcccgtttgc
     1981 ggctccggcc gttttgtcga aaataatttt aaatcaaagc gttgcatgga ctgcggcttt
     2041 gtgtattatt taaatattgc ggctgcggtg gctgcgttta ttctaaaccc ggcaggggaa
     2101 cttttgcttt gtcgcagggc aaaagagccg gctttgggca gcctcgactt accgggaggt
     2161 tttgtggata tgggcgaaaa catcgaagaa gcgctaaggc gcgaaatcta cgaagaattg
     2221 tcgctcaaag tggataccac gcggtacttg ttttcgttgc ccaatatcta taagtattcc
     2281 ggcatggaaa ttcataccct cgattttttc tttctatgcc ttgttagcga gtttgattca
     2341 attcaggcca acgacgatgt gggagaggcc tttttccgcc cgcttaacaa aatttatccg
     2401 gctgatttcg ggctggattc aattaaaaaa ggtattattg catttttaaa tgaaacccca
     2461 caaattcgat aatatatgct cagttacata aaaaggaaaa gcctgatttt tttgatgtta
     2521 atggcttttt ctgccggcag ttttgtctct gcgcaggaga acagtcaaaa aaaaggcccc
     2581 acctggttat accgcttagc gagcgaacaa ttcaatgccg gggaatacgc ttattgttta
     2641 cggactctcg atacctggtt tgaacaagag caggttttgc ctgaaaatct cagagaaaat
     2701 gcccggttta tgtacgctgc cagctcgtat gaattgaatc gcatggaaag cagcttgctg
     2761 ctcaaagaat ttatcggaga attccccttg tccgttcagg cttccagagc ttattatatg
     2821 ctagggatca atgctatgaa cgcccgtcag taccaggatg ccctggcttt ttttctttct
     2881 tgccgcagca aggatttttc taaaaaggag gaagccggct atcgttttta cctggctttt
     2941 acttatctgc aactcgatga attggaccag gcacgggctt tgttcgaagg tttattgcaa
     3001 gaagatcaac gctatgtctc cgcttcgacc tattacctgg cctttataga ttatcgtcag
     3061 ggccggacag agcaagccct cgaaggattc ggcaaaattg ccgcccatcc cgaatacgaa
     3121 cgcattgtgc ccttctatat catgcagctt tatttcgatc agggcaaaaa caaagaaatg
     3181 ctcgagattg ccgagaaact tctgcaggaa gatcccaatg aatcggagcg tattgagcta
     3241 taccgtcttt cggccgccgg ttattatgaa ctcaacgatc acgccctttc gcaggaatac
     3301 tacggtaagt acctcaggta caatccggag cttttgcgtt cggacgctta ccgcattggt
     3361 atcaaccatt atgtggccaa agaatacgaa caagcggcga agtatttcct gcaagtgagt
     3421 caggaaggcg atgcgctggc gcaaaatgcc gaataccacc tgggtttgtg ttatctgcaa
     3481 cagcaaaagt ttgaccaggc ccgcatgagt ttcgagcaag cttcgttgag cgaccaggac
     3541 aaggaggtaa aagagagcgc tctttacaat tacgccctgc tctgctatca aacttctttt
     3601 tcgcctttta atgaacaggt aagggctttc gaacgcatcc tgacggaatt ccccaattcc
     3661 cgttatgccg accgtgttta cgggcatttg gtcgatgctt tcttaagtac caaaaattat
     3721 caggcttctt ttgatgtgtt gaacaaaatt gccgatcccg gcaaggattt gctggaaacc
     3781 aaaagcaagt tgcttttttt gatgggtatg gatagattca acaatgcagc ctacggagaa
     3841 gctttgactt attttaactc ttcgattcag ttgagcaccc agctcagact ggaggctccg
     3901 gagacttatt tttggagagg ggaaacccat tatcgcctcg aaaatctcag ggctgcttcc
     3961 aacgattacc tgcgtttcct caactcacct aatgcttcga actacaaagc ctggcaattg
     4021 gcccattaca acctgggtta ttcctactac aatcttaaac aatatcccga ggctttgaat
     4081 tggtttttaa aatacagtca gttcaaggaa attcgcaacg ataatactta ccccgatgtt
     4141 ttgaaccgca ttggcgattg ttatttccat gcccgcaact acgatcgggc cgaagaatac
     4201 tacaacatga gcgacaagct ttcggaaacc ggcaacgatt acgctgtgtt tcagagaggg
     4261 ttcagtatgg gcctgcagaa gcggcacgaa gagaaagccc gtctttttgc cggttttgaa
     4321 aaacgtttcc ccctgtcgga ttacctggac gatgcccttt acgagcaagg ccgggcttat
     4381 atcgctctga atcgccctgc cgaggcctta cctgtttttg aaaaactgat gcaaaaattt
     4441 cccaacaata tactggctcg caaagctggt attcagatag gtttgttgga gtataaccgt
     4501 aataacacag aggcagccat tgccgcctac aaaaaggtaa taaacgaata tccgggcagc
     4561 caggaagctc aaactgccct taacgacctg aagatgattt atgtggccgt caaccgggtg
     4621 cctgaatatc tgaactacgt cgacaatctg ggcagcggtg tgcggataga acgcagcgaa
     4681 aaagattctt tgtcgtatct ggctgccgaa cgcctgatgg ttgatggtaa gaatcaggaa
     4741 gccataaaag gttttcagga ttatatcaat gcttatcctc aggggatgtt ccgtaccgag
     4801 gcttattttc acgccggaaa tttgcttttg cttagcaatg aaacaagccg ggctctgccc
     4861 tattttcaag aggtgattaa acaaaccggc catcgttttg tacccgaggc cttggtggtg
     4921 ttggccgagc atgagtataa acaaaaagaa tacgcccggg ctttggaaca ttatcagagt
     4981 ttggatgtgc tggccgaaga ccgcagtgtc cgcataggca ccagaagcgg aatattacgt
     5041 tgtctgtacg cgctcgggcg taacgaaagc attccggctg cggccgacaa gttgcttgcc
     5101 gaagaaaatc tctcacccag cctgcaacgc gaagcactgt tccggaaaac catcgccttg
     5161 atggagctga acagaaccga tgaggctctc tccgacctgc aacgtctggc cgaagagtca
     5221 cagaccgctt tcggcgccga ggcccgtttt cggctggccg aatattggtt caacaaaaac
     5281 gatctgaaaa aggccgaaga aattgcccag tctttcctta gggacggaac ttcgcaatcg
     5341 tattggctgg cccgttgctt tgttctcctg tccgatattt atattcaacg taaagattac
     5401 ttccaagcca ggcagtattt gatgagcctg aaagaaaact accgggccga agatgaaatc
     5461 gccggtatga ttcaaacgcg tttggataat atcgcccaac tcagtaagga atgattatga
     5521 aaaagaatac tctctgtttt ttgctggttt tatccggcct gttcacgatg tccgcggtag
     5581 ctcagacaga ccgggacagc ctgattagaa gagacctcct gctcgaaaaa gaatacaatc
     5641 ccatgctcga aacggccggt aagatttacc gtgtacccga aaccgaagaa cttaaaacta
     5701 agggaaccga agtgcgtttt tctttgtcgg aggcacccac tctgactccg ggagaatacg
     5761 ttccattgcg ggctgccgag gctcgcacct attttccggc ccgggagcaa aaggcttacg
     5821 tcaggctcgg agccggctac gattggtcag gtttgggcga cctccaatgg aacatattaa
     5881 aggggagcag gcatctgctc gatttcaccg cctggcatcg ttcgtctttt ggaaaagtca
     5941 ctcttcagcc tgatttggga accagagcca agcatataga caatcgtttg gggctcaatt
     6001 accaatggca tttaagttcg gcggaattca gggcaggctt gtcggagcgt ttttacgcct
     6061 ggaattatta cggccgttcc tggagcaatt tgcccttgca aaacagagac agcttagtgg
     6121 tgccggattt gcaatggttt tctgacacca agctcagtct cggaatagaa tcaaaacctt
     6181 gggacaaagc cttcgattac gccttcaatg tggatgctca ctattttcgt ttgggaaaag
     6241 ccctggccaa agacagcctg gccggtgccg gccccgccga aaccgaaata gatgccggtc
     6301 tagccttaaa ctaccaattc aacgataatt tggatatagg tttggatgcc cgattcagag
     6361 ccttctttta tcaacgcggg ctttcttcac aaagattcag cctggccgat catatgcagt
     6421 acgatccgct tttcccggcc tataacgatg cgccggccaa tgtgttatgg tttgaaatga
     6481 atccccacgc aacctattcc ctgggtttat gggattttgg cctgggattt cgtttgtctt
     6541 atttggacgg acaaaaattg ttgattgctc ccaccgcctc tgtggaccga cctttgggta
     6601 attgggccaa actcaacgtt ggaattgatg gaggacagag actgtattcc taccgcgaag
     6661 gcctggaact caattcttat ctggatcctt ctattcgttt gttgcccgag tacagcccgg
     6721 tggcgctgag gggagctttg caggttaagc cgttttcggg tctcaatatg gccgtattca
     6781 ccggctattc ttttaccaga gaccttgcaa tgttttacaa tgctctgcct gtagacagcg
     6841 gcctgttgaa tcacttgagt tatagtagtc tgtttatggc cgattatgta aaaatgaaca
     6901 aactttatct gggcctggac tttaattatg tctataggaa gaccctgaat ttgagcggta
     6961 aaatcagcta taatcactac attgcttcgt cggacaacgc caagacagat atattgttag
     7021 aaagcaatgc ccgcaggccc tggcataagc ctgctttcga aggagaggct ttgctcagcc
     7081 tgactccggg agagcagact gctttttttg cctcctacca ttgggagggt gtccgctatg
     7141 ctcccgatta ttttaacacc taccggagtt tgcccgatat ccattatctt tcgctgggag
     7201 cttcttacga cattacccgc cggctgggcg tatttttgca tctgaacaat gttctgaatc
     7261 aattctatga agtatggaat gcttatccct ctcagggctt cacggccgtc gtgggggttt
     7321 cctattcttt ctaaactcgt atgctggttc gaagtcaggc tcttgtgttg catcagattc
     7381 cctacagcga tgccttttcg gtggtgcatc tctataccag ggagtttggg cggcaagcat
     7441 atctattgac aagaagtcgc aaaaacagca agctgaggca gtcgctcttt cagcctctgt
     7501 ccctgattga gtacgaagcc gatcacaagg ggagccggga attgcagcgg ctcaaagaag
     7561 cccgcagcct gtttgcgttt acaggaattc cgatggatcc ggtaaaaaat gccatagctt
     7621 tgtttttatc ggaggttttg taccgggcct tggaggaaag agagtccaat ccggccttgt
     7681 tcgattttct ggtacaatct gtagaaatac tggatctttg ccgcgagggt gctgcaaatt
     7741 ttcacctggt atttttgatt aaacttaccc gttacctggg cttttatccg aatttggaag
     7801 acaaggcccc cggtaaatat ttcgacttgc aggccggtac tttttgttcg ctccaacctt
     7861 cgcacagaga ttggttgtcg cccgaagacg ctcagagctt tgctgtgttg ctgcgcatta
     7921 acttcgagaa tatgcctgct tttcgctacg accgttacca acgtcagcgc atattgcgcc
     7981 aaaccctcga ttattaccgt ttgcatttga gcgaattccc caggataaag tcattggatg
     8041 tattgcagga attattctga tcattgcatc gaaatatgct gtgagaaata ttccatcgga
     8101 ggtatcggct aagataatct tgttcaatcg gaaatttagc cttatttttg cgccctaatc
     8161 aataagtatc ttggtcctgt agttcaatgg atagaacgga agtttcctaa actttagatt
     8221 cgagttcgat tctcggcggg actactttga agcaacaaga aggctgtttt gacccgcggg
     8281 ttggaacagc ttttttgtat cactctgttt ataaaataaa tgtagaggct gaataagatt
     8341 gcttcccgtt taattgaaaa ttaaattagc aatcagaaat ctgaaggccg aatctccggg
     8401 ctgttgtccg attgattcag tcattttaat tcgaaaaagg gggtaaaagc ggtagatttc
     8461 ataaaaataa aaaacatcga tgaaatcgag gatttataat tttgacttta cacacaagta
     8521 ttattcttgt ccggatttaa gcgaaacttt ttgatgttaa ccaccagaat aacaacaata
     8581 tacagcgaaa cttttttgga ttacagaaaa tttaacagtt tttcgatgca tctttgtata
     8641 tgtccttcga tgcttcaaga aaaagagagc tgtgtgcgtc cggtttcggc gtcgagacat
     8701 ttagtggaat gaatattttt tggcagttat aaattagttg tgatgagttc tggtagtagg
     8761 ttccctttgg ttgtttcggt tgtttcgtct gtgttggatt atttgtttcc tttcttaggt
     8821 gaaagggagg tgggggaaat acaacaaacg aaaagaccga aaccggatta ccgcaggcaa
     8881 aaaagggcgt taattttaaa gtacgggaac cccacctata acctggtgct taaggagcag
     8941 aatctgaacg aggaaataat aatctttggg aagacaagac ggataatgat acagggaaaa
     9001 ctattctccc actccgatat tttgtcgtgt acatataccg atagtcaaag aattcttaag
     9061 ggcgaggcta cttttgtggg taaattggat acctgtaaaa tgccttcgtt cagaggtttc
     9121 gaaggcgata ccaacctggc ggcatacttc gaaggcaaca atgtttccaa taaggaaata
     9181 attacacaca attatactgt aatcataaaa ctcagagatc tctcggcatc cgttctaaaa
     9241 atccccatgg gatcgaattg ggaaaaagta aatgagctgt tggaggcgat aaaattcttt
     9301 atatcctcgt aacctcagat tacattgggg tgttatgtaa aatataagag gatagtaagc
     9361 caaatctata caaaagacag aattgcccga ataagcattt gtccggctat aaatcatcaa
     9421 atgaatttct aactttgggg agattacttg attttattat gaataaaaga aatttccctt
     9481 taatcggtct ggccgttttc ttcttgcttt tgccctgtac tgttagggct gaaaagaaaa
     9541 agcagataga attaatggcc gttaccaaag tggtggatgg agacacgttt tgggtggacg
     9601 atggtagcga aaaaggcaaa aaggttcgat taattggtgt agatacgccc gaaacattgc
     9661 atccgcaaaa agctccggaa ccctatggtc gtgaagcctc cgaatttaca aagtctctgc
     9721 ttgagggtaa aaaggtaaga ttggaatttg atgtggacag tcttgatcgc tacggccgct
     9781 tattggccta tgtttacctt gaaaacggac tttttgtcaa tgccgagctg gtacgcaaag
     9841 gttacgcaca ggtggctacg ttcccgccca atgtaaagta tgccgactat tttcttaagt
     9901 tgcagcgcaa agcccgcaat cgtaaattgg gattgtggag agagtaattt ttgagatgtt
     9961 tcgcctttgg gataaaaatc aatgtaaaaa cggacagtag ccggcttttg taggcagagt
    10021 tgtttttttt attcgtaaat catttttttc gtcattcccc cgtctattac caagttttgc
    10081 ccggtagtaa atccggagct ttcacttagc agaaaaaagg cggcttcggc gatgtccgtt
    10141 acctttccca cccggccggc cgggtgttgt ttgtggtcgg tttcggaaag cttttcgtag
    10201 tgtttggttt cgatccagcc ggggctgatg caatttacgc gggttttggg gcccaggcta
    10261 ttggccattg catgcgtcag tgccactatg ccgcctttac tggcgccgta ggcttcacca
    10321 ccgggttcgg acatcaaagc tctggtggag gcgatgttca gaatggcaga attttcgctg
    10381 aataggggca ggcaggcttt gctcaggaaa aacacactgc tcagattcac agccaatatg
    10441 cgattccagg tgtcaatgct catactatcc ataggttcga agtaactgat gccggcgttg
    10501 ttgaccaagt aatctacttt gccgtacagg gcttcgattt tggccattgc atcgtttact
    10561 tcgtccggat tggctacgtc gcaaagcaaa ccaaaaatgc cgctgttttc tgcttccaaa
    10621 gcctttagtt gttctgtatt ggaatcgagc acaacgactg cgcaaccttc tttcttaagc
    10681 ttaagagcga tggctttgcc tatgccctgt gctcctccgc taacaatggc agttttcata
    10741 attacgttat atgaaagatt ttgcaacaaa cttaatgaaa taacggcaat attgagtttt
    10801 atttttgatc tttgccggag attccggaaa taagccggag actcctgaaa cacagataat
    10861 actgaataca cagaaaatgc tgaatacaca gaaaataccg aatacactgg aaacacccaa
    10921 aacaccgaaa aatcgcaaac acggaaacac tgaaaacacc ggaaacaact tatttaaatc
    10981 actttcaatt actttctatg ataaacaata agattatgct tcgtactttt tttgtttggc
    11041 tgacgatgac agggattgtc gtcgccatgt cggcacagga aaccaatgcc gaaaatccgt
    11101 ccgaaaggca gcaaacccat tttgaatgga ctgttcaggg caacccgatt gttacccata
    11161 aatatacggc cgatgcggca gttatggtac gagaagatac cttgtggttg tttaccggag
    11221 aagatgctgc aggtaatcaa aatcgctaca tcatgcacaa ttggtgtgtg ttttccacca
    11281 cagacatgaa acacttcaag gaacatccta tccctctgta cggggccgat ttcaagtgga
    11341 gttcccgtca ggcttatgcg ggtcatgtga cggagcgtaa tgggaagttc tacttttacg
    11401 tgagtactaa tgcaacgggt ataggcgtgg caatagcaga tcgtccggaa gggccttaca
    11461 aggatgcttt gggtaaacca ctgctgacca atgccgattg ttttgccagt acacacagct
    11521 gggcttgcat cgacccggct gtatttattg atgacgatgg acaggcctgg attttctggg
    11581 gcaatcgcga gtgttactac gctaaactca aagaaaacat gatagagatt gacggccctg
    11641 taaaacaaat tatgttcgat ggattcagct ttaccgaagc tccctggata cataaaaaag
    11701 acgggaaata ctacctgact tacgcttcgg gctttcccga aaaaatacat tatgccatgt
    11761 cggaaagtat cgaaggtccc tgggaattca aaggcatact gaacgaaata gccggcaatt
    11821 caaacaccaa tcaccaggcg attgtcgaat acaagggacg atggtatttt ttctaccaca
    11881 acggagcgat gcagacccag ggtaccagtt acagccgttc ggtttgtgtc gattatcttt
    11941 attacaacga ggacggcagt ttaaagcggg tgcagatgac cagcgaaggg gtggatcata
    12001 taccgggcga aaaggatgcc gctttcgata cgttggccgt caacggtgcc tggtgctggt
    12061 ttgccgatcc cagagccatt tatcataaag gagaaaaaga acaaacctat ttttcgtggg
    12121 ttaccacttc gggcgatata gtggtggctt cgtacaatca tcaaaacggg gaattcattc
    12181 aacatgtggt cctggagaaa tttcagtcgg acgatcacgc caatcctact ttatttattc
    12241 gtgacgacgg gcgcatcatt gtttttgtgg cggctcattt cgggaatacg atcaaacgat
    12301 gtatcaccac caaccctgaa gatatcagct cctgggggga ggtgtatgaa tttggacata
    12361 ccgtcaccta tccttatccc tttcaactgg gtaaggattc cattttagtg ttcttcaggg
    12421 gcggcagttc ctggaagccc catatgattt tgtccaccga caacggcgaa acattcggca
    12481 ctcagaagga attcattgtc agtaccggta aacgccccta catgcgttat tgccaggggg
    12541 cagacggtag cattcacatg gcggccacca cgggacatcc cagagacgtg ttggataata
    12601 agatctttta ttgctgcttc aaaaacaatc gtttttaccg ggcagacggt tctctgatca
    12661 aggattttgg ccgagaaccc ctcgacgttt cggaaatgga cgaagtttac gaaggtattt
    12721 cttgtggcaa gggctggatc tgggacatag ccctggaagc tgaaaccggt ttcccggtaa
    12781 tggtttatgc ctcctttccc gacgataccg accaccgcta tcattatgcc cggtggaatg
    12841 gtaagaattg gacaagtacg gagctggcca aagccggcaa atggtttcct cagacaccta
    12901 aaggaaaaat cgaacccgaa cccaattatt caggaggtat tatcctggac tacgacgatc
    12961 cctcgatcgt gtatttgtct aagcaagtgg acggagtgtt cgaactattt aaatatacca
    13021 cacctgatca cggacaaagc tgggaatcgg aagctcttac cgagaacaca cccaagggct
    13081 tgctgaatgt aaggcctata gtgccgcgtc atcacaaaaa aggtgttttt gatgtcatct
    13141 ggatgcgtgg ccgttatgaa ttttatgccg atgaacgcta caatacctct ctggttttcc
    13201 caaaaatgga aaagaccggt gaattggagg aaagagattt caacacgtcg ggttctccgg
    13261 ctatttaaac ggataatatt ccgactgttc tgaaaattat gcgttttggt aatacaacgt
    13321 agagcagttt tcgggccgga atacattttc ggccatggat cggagatcct cgccgcttaa
    13381 tttttttaat ctttcgggtt ccagcaagta attgtcggca gaaccgagga tttcgtacca
    13441 gcaagcctgt gtagccaggt gtctggcatc ggtgttttgg aataaaaaag ccgattcgtg
    13501 tttattgagt attttattga gctcttcctg ccctggggct tccgtggcta tctgattgag
    13561 acaggcttcc agggcttttt cggcttcttc gaggctaacg ccctttgcag gtttgcctga
    13621 aaaaagaatg ctgccggctc cggccagtcc ttcgccacca acataaatgt ctgcctggct
    13681 gaatacctga cgctctttga cgagttgttg atagagtctg gaggaattgc ctagggacag
    13741 ggcatcggtc agaaggtcgc aggtgatgta ttcttcttcg cagactgcat atgcccgcca
    13801 gagtttatac aatgccgggc cgggaacggg gcgttcaact tccagaaaac gggggccctc
    13861 ttgttgaggc caggccggaa attcaggttt gtctgtttct gccgatgcta ttgccccaaa
    13921 gtattgttcg ctaagttttg ccaggtgttc gaaatcgaaa tcgcctgtaa tggccagcac
    13981 agcattgtcc gggttgtaat ggcggcggta aaatgcgtgt acatcgtcca ggttgaaggc
    14041 ctcgatatgc tccgtagact ttccgatggt gggccaggaa taaggatgcc cctcgtaggc
    14101 cagtgagcgt aaaagcatgg aaacatctcc gtagggttga ttcaggtggc gttgtttgaa
    14161 ctcttccaac accacttgtt tttgaatctc gaggctacgt tgattgatgt tgagcgacga
    14221 catgcggtca ctttccaaaa acagggctgt gtcgagcttg tgcgagggca gggtgatgta
    14281 ataattggta acgtcgcgac tggtccaggc gttgttttcg cccgaggctt cccgtatggc
    14341 cttatcaaaa tcgggcaccc tggcagagcc tgcaaacatc aggtgttcaa aaagatgggc
    14401 aaagcccgta tgatcgggat gttcatcttt ggaacccacg ttatacataa gattaacagt
    14461 agtcattctt gtattgctta tagggcagta caagagacgc aaaccattgc tcagccggtg
    14521 ggagaagaaa cgcatagtca gtttccgatc acctgcatac taaggattac cacatcggtt
    14581 ttgggacggt cggcagtgcc ggtttcgacc gactgtattt ttccgacaac ctccaggcct
    14641 tcgacaactt cgccaaagac cgtgtattcg ttgtcgagat gaggagtgcc gcccaggctg
    14701 gtataagcct tgatttgttc agggctgagt ccttggggag ggttagcctc gaaataagct
    14761 tccatttggg ctatcagttc attctgaagt ttttgcagac cggcctgatc gccgacttga
    14821 tacaactgtt ggattttgct ttgctgtttt ccggccaatt gttgaaaata agcatttttg
    14881 gcctggttga ttttttgagc ttccaactgg tcgatgtccg aggctttgta aacctgtccc
    14941 cagacaatat aaaactgaga gccggaggag gctttttgag gattcacctg gtctccggtg
    15001 cgggcagccg acaaagcacc acgcttgtgg aagtattttg ggtaaacaaa ttctgcggga
    15061 atcgtgtagc cgggaccgcc ggaacccaat tgtttgccgg caggggcatt gcgcgattcg
    15121 gggtctccgc cctgaatcat aaagtctttg atgacgcggt gaaacagcag gttgttgaaa
    15181 taaccttcgt ttaccaactt aataaaattg tcccggtgct tgggggtttc gttgtagagc
    15241 ttgattttga ctttgcccat ggaagtttcc atcaggacaa aagttgcttt ttcttcggaa
    15301 tgcatcatgt gagtttggct ggtaaggagc agtacggaaa tgaaaaaaaa cagttttttc
    15361 atgtttaatt tattttaagc cccgcaaaga tatgaatatt ctgagtacct ttgtgttcac
    15421 taacccaaaa aaagaactat gaccaaacca acactatttg ttttagcagc aggaatgggc
    15481 agtcgttacg gagggctcaa gcaattggac ggagtgggtc cccgtggaga aaccattatg
    15541 gattattcca tttttgacgc agccagagcc ggattcggca aagtagtctt tgttattcgt
    15601 aagagtttcg aagaggattt taagactaaa attgtgagta aataccaagg tcttatgcct
    15661 gtggagattg tttttcagga cttgaacgac ttgcctgccg gctttgtctg tccacccggg
    15721 agacaaaaac cctggggaac caatcatgcc gtgttgatgg gcaaagatgt catcaaagaa
    15781 ccctttgcag tgattaatgc cgatgatttt tacggtaagg aaagttatgt ggtcctggca
    15841 aaggccttga acgagatgca agggaaaaag aaccactatt gcatggtcgg ttacaggata
    15901 ggcaacactt tgtcagagag tggttcggtg gccagaggaa tatgtcagac cgacgaaaac
    15961 aactgcctga ccaccgtggt ggaacgcacc tggataattc gcgaccaaga cggtttggtt
    16021 aaatataaag acgaaaacga gcaattggtg acagtgggcg aaaacactcc cgtatctatg
    16081 aatatgtggg gttttactcc cgattatttc gattattccg aaactaattt catcgagttt
    16141 ctcaaagaaa acatcgacaa tcccaaggct gaattttata ttccctgggt ggtaaacaat
    16201 ctggttaatc agggtaaggc tgtctgtaag gttctggata ctccttccaa gtggtttggg
    16261 gtgacgtatg ccgaagaccg ccccacagta gtaagtaaaa tcaaagaact ggtcgaggcg
    16321 ggagaatacc cttcgaaact ttggtaaaaa attaaggaca tcagcaatat aaaacattag
    16381 caatttaaat cacatggatt acacagctat ttcgacacaa ttcaagaaac gttacggctc
    16441 aaaatttagc ctgtatgcct ctccgggcag ggtaaacctg atcggcgaac ataccgatta
    16501 caacggtggt ttcgtatttc ccggtgctat cgacaaactc attgttgcag ccatcaatcc
    16561 caatgggacc gatatggtca gagtgtattc ttacgacatg aaagaaacag ccgaatttgg
    16621 tttagacgaa agacctattg cttcctgggc cctgtatatc tacggtgtaa tcagagaaat
    16681 cgttaaacgc ggtttgcggg ttggcggctt cgattgcgtg tttggaggag atattcccat
    16741 aggttcgggc ttatcgactt cggccgcttt ggaatcgact tttgcctttg ccttgaacga
    16801 tttgtataag ctgggactga ccaagttgga tatggccctg atcggacagg ctgccgaaca
    16861 caacgccgtt ggagtgcgtt gcggcattat ggatcaattc gcctcggtgt ttggagagac
    16921 agatcacctg atacgtctcg attgccgttc gctggaatac gaattggtgc cttttcatcc
    16981 caaaaattgt cggttggtct tgctcgatac ccaggttaag cacaacctgg cggcttcgga
    17041 atacaatata cgccgtgcgg aatgcgagga gggggtgtct attattcaaa aaaagcaccc
    17101 gcaagtaaca ttactgcgcg atgtcagcct tgatttgctg aacgatttca aaaaagagct
    17161 cagagagagc acctatcgcc gttgtcgcta tgtcatcgaa gaaaaccagc gtttgctcga
    17221 tgcctgcgag gcccttaaac acgatgatta cgcggaattc ggaaaaaaaa tgtacggttc
    17281 gcatgagggc ttaagcaagg aatataatgt cagttgcgag gaattggact ttttggtaag
    17341 tattgccgtt aaaaacggag ccctgggtgc acgtatgatg ggcggaggat tcggcggctg
    17401 caccataaat cttttggaag acagtcatta ctccgacttt atcgaagagg ctattgccgc
    17461 ctacaaaaaa caattcaacc gtgctcccaa ggtttacgat gtcaaaatcg gtaggggatc
    17521 gcataaaatc gcctgataat gtatttactg ggttacgata taggaagttc ttcggtgaaa
    17581 gcttctttgg taggtgcgca gagtggcgaa tgtatctact ccgccttttc accggtcaca
    17641 gaaatgtcga taaaggccct gcaagcaggc tgggcagagc aagatcccga agattggtgg
    17701 cagaacctca aaattgtcac taaagaaata ctgttaaata ccagggttga agcgacttcg
    17761 ataaaagcca tcggtatctc ctatcagatg cacggcttgg tattggtcga caaacaacag
    17821 aaagtattgc gtccggccat catctggtgc gattcgagag ctgtgcctta cggtgaaaaa
    17881 gcctttcgcg acctgggaga ggagcgttgt ctttcgcatt tgctcaattc tcccggaaac
    17941 tttaccgcag ccaagctggc ctgggtcaaa gaaaatgagc cggagctttt taagcagatc
    18001 gataagattt tgttaccggg agattatatt gcacttcgcc tgaccggaga aacctctacc
    18061 acggcttcgg gtttgtccga aggtattttt tgggacttta agctgcatca gccggcagat
    18121 tttatactgg attattgggg cataccccgg gagtggattc ccgtgatatt gccctctttt
    18181 tcggaacaag ggcgtttgtc ggcttcggca gccggtgaat taggcctgtc caaaggcact
    18241 tccatttgtt accgtgccgg tgatcagcca aacaatgctt tgtcgctgaa tgtgttgaac
    18301 ccgggtgaga ttgcagccac cgcaggtact tcgggcgtgg tctatggtgt gaacgacagt
    18361 ctggcttatg atccgaaatc gagggttaat agttttgctc atgtcaatca cagcaaagag
    18421 tccattcgta taggcgtttt gctttgcatc aacggcaccg gtatattaaa tgcctggctc
    18481 aaacgcaact ccgcctcaaa cagcctgagt tacgctcaat tgaatgaatt ggctgccacg
    18541 gcgcccatcg gtgccgacgg cctgtcgatt ttgccttttg gtaacggctc ggagcgcatg
    18601 ctcgaaaaca aagcttcctc ttgcagtata cacggtttgc aattcaaccg gcattctcag
    18661 gcacatctgc ttagggcggc ccaggaaggc atcgtatttt cctttgccta tggtatggaa
    18721 atcatgcagg cgatgggaat caatactgcg ctgatcaggg ccggtcatgc caatatgttt
    18781 ctaagtccct tattccgcga aagtctttcc actcttacag gtgccgctat tgagctgtac
    18841 gataccgatg gttcggtggg agcggccaga ggtgcgggaa taggttccgg tgtttatctt
    18901 acaccacagg aagctttcgg taaactcaaa aggatagccc gaatcaatcc caaacctgct
    18961 gagcagcctg cttatctgga agcttatgat cgctggaaac aatacattta gtcatatcaa
    19021 cacaaaacaa ataattatgt caacaaaaga attctttccc ggtatcggga aaatcaagta
    19081 cgaaggacct caatccaaaa atcccctggc atttcgttat tacgatgcca acaaagtggt
    19141 aatgggcaaa accctggccg aatggacccg gttttcgatg gcctactggc atacgctctg
    19201 tgccgaaggc ggtgaccagt ttggcggagg cactaaaacc ttcccctgga acaagggaac
    19261 ggtcctggaa agggccaaag ccaaaatgga tgccggtttt gagtttatga gtaaaatagg
    19321 catacaatac tattgtttcc acgatgttga cctggtcgac gaggccgaaa caatagagga
    19381 atacgaagcc aacctcaaaa ccatggtaga atacgccaaa aaaaagcaag ccgaaaccgg
    19441 aataaagctc ttgtggggca cagccaatgt gtttggtcat aagcgttata tgaacggtgc
    19501 tgcaaccaat cccgagtttg cttcggtggc ttatgccgct gttcaaatta aaaatgcgct
    19561 ggacgctact attgccctgg gtggtgaaaa ctacgtgttc tggggtggtc gagaaggtta
    19621 tatgagcttg ctcaataccg acatgaaacg tgaaaaagct catttggcca tgatgctgac
    19681 caaggcacgt gattatgccc gtgccaaagg attcaaagga actttcctga ttgaacccaa
    19741 acccatggaa cctaccaaac accagtacga tgccgacacc gaaacggtta ttggattttt
    19801 gagggcccac ggtttagaca aagattttaa ggttaatatt gaagtgaacc acgccactct
    19861 ggcaggccat acctttgaac acgagctaca gtgtgccgcc gacgccggca tgttgggcag
    19921 tatcgatgcc aaccggggcg actatcaaaa cggttgggac accgatcagt tccctatgaa
    19981 cctgaatgaa ttgactgagg cttggttggt tattttacaa gccggcggcc ttggtacagg
    20041 cggcaccaat ttcgacgcta aaactcgcag aaattccacc gacctggagg atattttcat
    20101 tgcccacata gccggcatgg atgcttttgc ccgcgctttg ctcgccgccg ccgatatttt
    20161 ggaaaaatcg ccttacaaaa aaatgcgtaa agaacgttac gcttctttcg atcagggcga
    20221 aggtaaggct ttcgaagaag gtcgcctggg actggaagaa ctcagggcat tcgctcttaa
    20281 aaacggagaa ccccgccaga taagcggtaa acaggaattg tacgaggcca taataaatat
    20341 gtatttgtaa taatcttctt ataaaaaact tagccccgaa ctggaccaat cacggttctg
    20401 ttcggggctt gagtttgagc taaagcagtc tgtttatagg attactttca cgacatcgtt
    20461 gtttgtctta accaaataga tttgtcgtgt attcaaacca tcaatagcat taactccttc
    20521 attgacctga acgcaatgaa ttaatttacc caggacgttg tagatttcta ctgtttgttt
    20581 actgtcggtt tgtatgtata gtacaccgtt cttggtaaac acattcctgt ccttgccgga
    20641 aacacttggt ttactaatgc cgtcaacatt aatataagta gctttggctc caacgtaggt
    20701 gccatttaat ccggcggtat acaagggtga agtacttccg tcaatttgca atttatcttc
    20761 gttgtagaat acgctgtcga ggaacaaagt agacgtgaac aaacgttcga aggttacctt
    20821 ggtttcgtcg ccttccgaca ccggtaaact gactgtttta cccttttggg cacataccga
    20881 gtcgggagcg aagaagttct tgagaacttg aacatccaat acctgatcgg tatcgggata
    20941 gtacaaggca aggttgctta tgggatgatt ctgattactc caacgcttgt agaacaggtt
    21001 gttgttgatt ttgatgtagg catcaaaacc ggccggtttt ttgttgaagc ccaagaattg
    21061 tctggcagaa gcaccggtgc ctcctatttt gtagaaggtg ttgttctcaa tggttatata
    21121 aatggttgaa tctcccttat gcgagggtga accttctctg gtaatgttga agaactgact
    21181 tccgtggaag tttttgacga tattttccct gaaggtgaat ttatccaatt cataagtagg
    21241 attgctcaag aattggaaga aatgataacc ctggtttccg gaagctttgt tccagcccat
    21301 attgtcaaaa gtacagtatt caaacagcac ctcattaatc aacgcattgg cgcaattgtt
    21361 ggttctcatg atgggctgat agtccagatt ggtgaaagta caattgcgta caataaaggc
    21421 gtccattata gagtcggcag tactgatata gaaaggagcg tagctgtcgg aattgaaatt
    21481 gactatgccg ctatcgccac cgaaccagtg caggccttct accaggaagg tgttgagttt
    21541 cgtgccgttg ttggacgata agcgtccgaa gattgtaggt gaatggcctt cggctgctct
    21601 gatagtcagg ttgccgaatg tttgcgggaa ggccataccc tgtccaatct gatagatacc
    21661 caaggtatcg gtaagattgt tttccacttc gataacatct cctccaatgg caatactcaa
    21721 agctgttcta aattcaaagg aattgctaac tctgatgatt ccgctttcgg ttctgtccat
    21781 ataacgctca acaattccta ccggggtacg tcctgttccg cttatgaaga ggggcgaacc
    21841 ttttgagatg gtcagcgctt cttcgtcaat aaatacatcg gtaatgccca gaccggaagc
    21901 agtcaagagg ttgtagctcg gggtgacagc actggcttcg ctaagggtaa gggcctgtcc
    21961 gtcaacaaca gatacaacat tattgggata gaagaagttg ttcaaaactt caacctcgat
    22021 caactgcgtg tcggtggcca aagctgccgg caggcttata tatgcaatag gtgcatgttg
    22081 ttcgctccaa cgcttgtaga acaagttgtt gttgatctta acttttgcat taagaccgcc
    22141 cagcgctcca ttgtaaatca ggaagtgctt gttggcggta gcattacctc caatcttgta
    22201 gaagatgttg ttggcaatga ctactgagaa gtttggccat acggaatcgt attcagcggt
    22261 ggttcccttc ctnnnnnnnn nncctgggga tattgaacag gtggttaccg tggaagttct
    22321 taacgatgtt cttgctgaac aacaggctgt cgcattgata gttggcagca tcagagaatt
    22381 cgatcaaagt aggaccgtca acacctgagg ctttacttcc tcctatgttt tcgaagaggt
    22441 tgcctctgaa aactacggca ttgataagag ctcctgcaca attgtcggtt ttcataacgc
    22501 ttgtgctgtt gaaattcttg aacgtacaat tctcaacggt aatgccgccg gtaagggtgt
    22561 cggccttatt gaaatagaag ggctcgtaag aggcattgtc gtagttggct aaagccgtat
    22621 tcccaccgtc ccaagtcagg ttcttgaagg tcaggttgtt cagcttcata ccattcgttg
    22681 cataaaaacg tccccagatg ctgggagttg ctccgctagc gggctgcaat accaaatcgt
    22741 tggacgaacg cgggaaaatc attccgctgt tgccgatttc gtatacaccc gaggcatctg
    22801 tgctgttttt gagcttaatg gtaacgatgt ccagatttaa agcgctgtaa tcgtaagcca
    22861 gagctgctat gagttcttcg gtattttcta cggtagctcc cgaagaggtc atgttgaagt
    22921 tgggtacagc aaatacatca cccgagccga tgtaatttac agcgttgata atcaactgta
    22981 caccttcttc gctgacgttt tcgtagtttt cggcagaaag tccgatcatc atatatttag
    23041 ctgcgggatt ggagttgatt tcctgaatac aagccaagtc gggactttgt tccattacag
    23101 ccagcgtata tgcctgagga cctttgtagc tgctaaagcc ttgcaagcct ttgaagtcgt
    23161 tggatgttgc cccacggctc aggaataagg tatcgaacac attgaaagcc ttcaggctgt
    23221 caagctcaat atttttgaat acagggtgga ataccatgtt cgactgaact ttggcgcgta
    23281 attcaaccgg cgtggcctga tcgtatttgc cattgccggc gacaccccaa ttccaactgt
    23341 tgctgttgta agtcattgcc tttaagttca acagaggttt gttgccaacg agatatttgc
    23401 atgctttggc aatgtcggaa tcggcatcgg ggaattcact caagactact acgtcgtaat
    23461 cggcaaagag agcatttaca ccggcgctgt cgcttattcc ggcgtatatg tcaggataaa
    23521 cgtaggtcac gttgaaattt tccattaaag ccgggaggat aactttatcc agcgagtcgg
    23581 aggttatagc acggtcaaat gttttaatcc tgtctgcaat agtattgatg taagcaacgt
    23641 tgagtgcgcc ttctttgtgt tctacgtaat caaaaccgtt tatggagagg ttggccagta
    23701 tgaattgacc gttttcatcc agggctccgt tgttttgcag aatacggata gttacatagc
    23761 tttgattggt cagttcgggc aaatctaccg atacatgagt taagccatcc caatcttcgg
    23821 gagcggcaaa ttttccggca acggtaaatg tatctgcacc gtctacggca taagccacaa
    23881 cacaactgtc ggcagcatcg cgcaggttga agtcaaaatt caggttgatt tcctggaaat
    23941 cctgcagaga aacatcgatc tggaaataat tggcgtgctc ttcggccagg gtgaaatcga
    24001 tcgtgtcgtt tttaccggca ggagaaatca taacggcttt tacaccgcaa tctttaccca
    24061 attgcatgcc atccgaaggt gaagaagcag tgagcattcc tcttacaaca cccagatcgg
    24121 aggcgtacag ggaataataa tctgcttccc attcaccatc aatgtcggta acaaaagagg
    24181 tcaggtcgac tgttccgctc gctttgggcc tgtataagaa gtcccatccg gcaatggtgc
    24241 cgtcaaattc aggaacggat cctttttcga tttgtccgaa gattttgatc cggttgatgg
    24301 atgtgccttc gaaaaagccc cagcgctccg attttttgtt tttcatcgtg ccgtcttcgt
    24361 tttgatcggc agcattgacc agtctcattt ggaaggcgtt gctttgccat tctacaccac
    24421 tcgtatacca gtctttgtgc gggcctaaga caacgatgtc tgtagagtcg gtgccgggag
    24481 cgtgtttggg tttgaatcca aggttaaaaa cattccccca gttaccgccg atataccagg
    24541 ctgaatcttt tccgcgatgg agctcataag ccataatggc ataaaaggag tccgtaccgc
    24601 aaccctggat aaagattgag tcactggtag tgggaatacg gataacaata gagtcggtgg
    24661 gacgtccata aagccggcct cccttccatc tgttcacata aaaaagaggc ctttcttctt
    24721 ccggttgttc caaaaaggca gtgtagctga tgccactgta accgcttttc tggccgataa
    24781 ctatgcccca aagatcaact ttgcccttta aagtggcaac atctgttttg ggaggataac
    24841 cgtaatcgac aacagaattg ttgtgacgac cccatttgtt tacttttttc acgctcacgt
    24901 tgtcggtgat tgtcataaag gaaggaaatt cccaggtgga gcaatgcacc gagtcggcca
    24961 taataccgct gtcgtaaata atgtagtcgt tggggtcggt aatgatggca tccactttgg
    25021 gcactgccac caaaaacaac aacaaggtca ttaataaagg agtcaggatt tttttcataa
    25081 aaggtagaat taaaaagtta aacgtgttga caaattaaga gtaaaacaca aaattgggta
    25141 aaattagcgg tttttggtaa ttttctattg gtaaaaattg ataaaatatt gcgacttttt
    25201 ggtagggaag gtttttttaa ggatgattaa tagaaacaat tccctgtatg caagaaagtt
    25261 ttgatgtgtt cccgatttaa tatatacgcc aattaacctt gtaaatcaaa aacaaataca
    25321 attagcaatc tttagcagag atgttttaaa acttttctct aaattgcgcc ctattctata
    25381 tgaatagccc cgtcgaaaga gacattctgg ctttgcttca gtcccgactc gaagccagca
    25441 ttcgtctcgc tacaaaactt atttttcaac tacccgtgat aaacattaat taaacatagc
    25501 gatgaatgtc agaaacttaa ggacagaata caaagttaat cccctgggga ttgcaaacct
    25561 taatcccagg ctatcctgga gattggatac cgaaaaagaa aacagtcttc aaaccg
//
LOCUS       DAOSGL010000003        22067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_1897_length_22067_cov_4.115119, whole genome shotgun sequence.
ACCESSION   DAOSGL010000003 DAOSGL010000000
VERSION     DAOSGL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_22067_cov_4.115119"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2974
                     /locus_tag="PKY68_00190"
     CDS             <1..2974
                     /locus_tag="PKY68_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH23491.1"
                     /translation="KKTKEEALELAEAMVTGQRMHTDGSGFTIPEREISTAMQTINAK
                     EFEGLQVGTLDEALQGRVAGLDIVSTSGNPGSGSSMRIRGTASITGNKEPLIVLNGVP
                     YEVNIKDFDFATSNEEDYAELLSINPDDIEQITVLKDAASTAIWGSKGANGVLMVTTK
                     KGQAGPTLVQYTYRFTRSVQPKGLKMLNGDDYSMMIKQAYFNPRQDESAADIDEFKYS
                     DTFVEYENFNNNTDWIKAVSQTGRTHDHYLNVSGGGERATFRVSGGFYDQTGTIIEQH
                     LNRFTSKAYLDYAVSERLKFISDFSFTHVDNQRNYSNLLGTAYAKMPNVSIYEQDRDG
                     NDTDVFYNISRNSRLNAAQRDMINPVAIAKLGVNNLKSYRIVPIFQLHYNLTGERSRS
                     LRYQMYVSFDINNNKNSGFLPWEATNNTWNSNQVNRSESNDSESLSVLTENVINWQPK
                     LRNEDHSLTLYGALQTRTGNSGSQYIASYGLFSDKTKDASNEAYLEGVSTGRSNWRSM
                     GLVGRVHYTYKSRYIISGVVRRDGSTKFGNKRKFGNFPSLSLKWIISDEDFMADTRSW
                     LSMLAIRPNWGMSGREPGAEYLHFSRYAAYDSYLGKQATRPVSLRLSDLKWATLREWN
                     IGADIGLFRDKIVMDMNYYHRRTVDMLFSNISLPSTSGFASMSYANAGTMDNDGWEFN
                     VYTNRLVRRGDFSLDVNFNISNQVNTIIELDDRILANYNKDYDFRNGTYMTRLQEGHS
                     FGSIYGFEYEGVYQYDKYDEEHGHLDAPVARDKDNRVITDETGEPLPMVFAAGTTVEY
                     EFRGGDAKYKDQNHDGSIDELDIVYLGNCNPLFNGGMGFTLRYKTLSCTAFFNYRYGN
                     KVVNAARMNAENMYDNDNQSIAVNWRWRKDGDITGIPRALYNYGYNWLGSDRFVEDAS
                     FLRFKYLTFNYILPSNWLKPIGIDRVSAYLTFNNLYVFTKYSGMDPEIGYGDFGISTD
                     NAQTPRSKDFTLGLTIGF"
     gene            2987..4552
                     /locus_tag="PKY68_00195"
     CDS             2987..4552
                     /locus_tag="PKY68_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH23492.1"
                     /translation="MKNRLFIVSILALSLFATTACTNWLDTRPESEMILDEYWTTESE
                     AEAVLMAAYRGFITSDCTYRMLVWGELRSDNFTQGTGIGDEINKVLRQEITPSTPYTT
                     WSSFYSVINYCNTFLSFAPDVLKKDDNFTVEKLRKLSAEAYAVRALAYFYLVRAFQNV
                     PWISEPSLDDTQDFIVPQFSERAVLDSIIRDLELYSLPYIPRIHSTDKETKGRFTKNG
                     VYALLADIYLWDNQFEKCSQMCDYILADDYYTLVPAENFYSQVFYSGFSSESIFEFGY
                     DNNIQVNDATRSLYGYQNNQNGQLAFPVTLVSGQYSPFEYPAGSYIESKNDVDIRWKD
                     FFVNDPGVGVYQIFKYAGIQRTENFDGSRSTYSYRNNTSNWIVYRLPDVILMKAEAVL
                     QGKQEEGLAEALSLVNMTYSRSNGGDTLSLDYYDNSYDKLVELVLRERQRELMFEGKR
                     WFDLMRMARRAGSPAPLVSFIARKGEGSEALGKMSQMNALYWPVNKGELEANTALVQN
                     KFYETESTTSTLK"
     gene            4565..6565
                     /locus_tag="PKY68_00200"
     CDS             4565..6565
                     /locus_tag="PKY68_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH23493.1"
                     /translation="MKTTNAFKKKILYLLLLFSISPGLFQSCYTEDVVDNYFTFTGEL
                     IGDYIDNRPERFSEFKRLLDTTGVMGLLKAYGLYTCFLPTNDAMQKYYQDHEKNGAED
                     FSLAELKTICYNHIIKGDTLATMQFSEGALSSMSMSGRFISVDFSDTTATIFMNGTSP
                     IIEKDIILHNGVVHVLSKAMEPSQDRVSSKLMHDPKFSLFAKAFELTGYRFIMDEAPI
                     EDLTYDPKNYTPSGFRHTVLEELPKYRKFGYTILAESDSTLANYTDCPKLPNGVKTLE
                     DLYTLAEYFYSSKPGNIGLFSDGDEVKDDYTDKRNYLNRYIAYHCMTRTLLSSRFIKD
                     YDTPHHFKIYDMYEYIETMLEDRLLEVKIDRNYSAPNSEFGFFNCKMAADGKEDASTG
                     ILLTDYKDNDAVNGYYHEIDKPLAYTREFEAALSAKRLRIEAACFFDELATNNMRGNA
                     KWNPNGSNHGEPDKTHAYQIPFGYMDRFFCTESTKFTYIGACSAYEDFQGDEMYARDS
                     YNFTMQTLPIPAGTYEVRMGYQPTAYRGIAQVYWDSIPTGIPVNFSLLASNPDIGHKV
                     PGSEADDPYGYENDKMMRNRGYMKGPDSYKCPVNIYSYSAANARYSTYNLRYILGTYE
                     FKEPAKHYITIVFLGTESGDSQFMMDYLEFCPTELILTEDTH"
     gene            6577..8112
                     /locus_tag="PKY68_00205"
     CDS             6577..8112
                     /locus_tag="PKY68_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH23494.1"
                     /translation="MKTNHSKLWPLIVLFLFALISCQEQAWYEHYEDSGKAGSEKTMM
                     EYIKSEPQLGLFLQMLEVSGYDTLLSVSQTFTVWAPQNNALSGIDLDDTTTVTEIISN
                     HIARYCIGTSSTRSDKVFMLSNKLVSFEGNKFGLTNLVSSNIKVANGLLHIIDGFVPY
                     VPNIWEYTGRIPGLDSLAAYFLSQNQREFNLLESKQIGVNADNQPIYDSSFIESNRYL
                     TERVNLNNEDAVFTALFPNNQAWHKSFDLIKDYYKTTDADGGWTKQKSLAQAALVKDL
                     VYLDNFKEKGIPDSLVTSSGHVIDSAKVLFQGAVSHDASNGVIYVTDSLRIPATSSWH
                     IPFQIEAEDNYYGRTSLNAKLYNRTSAGTSVSASGGRYLEVEATGTTILSKTYVDFPI
                     PDVLSAKYDIYCLFVPESVVNRINPKPNVVNLYMSYLGPDGKLKNNVSIKKNVTIDAE
                     DTTLVHVTQMDFPYCSVLDRNSEISDLTVKLRVENAVTASQTRLYTRTMRIDCIIFIP
                     VED"
     gene            8136..11294
                     /locus_tag="PKY68_00210"
     CDS             8136..11294
                     /locus_tag="PKY68_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH23495.1"
                     /translation="MKRNSSYMSRIILSLGLLWVAVTLGSVKAADKAAESKLFVKGQV
                     LSQATGSPVFGATVRAGESSVFTNEEGRFELIIQTANDVLVVTGDDFIAREYPLRGRQ
                     DVVIELYPRIFGENYKTVELPGGKTKSSKMISSAKTVDIGLNQAVSADELYHSSLGGD
                     IRTISRSGLPGIGSLLFIRGLNSLNAKAHPLLVVDGVIWNDISDVQSIFEGFQSNILM
                     NIDVNDIQSITVLKDGTSLYGSKAANGVVSIKTKRGESAVTKIDLRIMAGMVAEPGNI
                     PMMNADQYRVYASELLQTVNPGMLKTPKFLDFLIENPSDPIYNTYHNETDWSQEVYKR
                     GITQGYNISVNGGDQRALYHFSLGYTSNKGIVKTTEMERISSRFNADINVLDNLNVGM
                     NVGFSNIMRSLLDDGVNFYTSPTYMGYAKSPFLSPYSFTSLGYKAKDYDDADLFGIGN
                     PSSFIAGSQNKYKEYRFNVGFMPVYNINKHWTLSTLFDYSLNKYLEDYFSPMHGSYPR
                     ELEGFGMSENTVKNQLIRNTAIFDDTRLSYHLLKKNFEFKTLLGWRYMTNYLEADYIE
                     SHNSGTDNNTTVGNYDFLQTHGLNNRTKSVSTYANVDLAFLNRYFINAVLSLDGSSRF
                     GKEAKGGVELFGHSFALFPAFNAAWLISSESFMKNIRFIDYLKLRAGYDFTGNDGIQD
                     YATKAYFVTRRYIGQANGVILSNLANEELQWETTQSMHAGLDMTLFNERLLLNVDVFD
                     SNTDNLLTLRSLNQIAGVDYYWSNEGKLSNKGFEISAETKLVNTKNFQWSLGASIGHY
                     RNLVVELPEKGYTTPAYGGEIRTEVGQPAGVFWGYKTQGVYASEADALANPMAIVNDN
                     GSLTYFKAGDVIFEDFKKDGILDENDKQVIGDPNPDFYGNFSTKLSFKKWTLNTLFTY
                     SYGNDVYNYLRSQLESGKDFSNQTKAMLLRWTAEGQNATLPRSVYDDPMGNARFSDRW
                     IEDGSFLRLKSVSLSYTIPIENVFIKGLEFSLSANNLFLFTNYLGLDPESSVSDKVLF
                     QGIDNGLIPQTRSYFLGVKVNL"
     gene            11327..13036
                     /locus_tag="PKY68_00215"
     CDS             11327..13036
                     /locus_tag="PKY68_00215"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH23496.1"
                     /translation="MKAFKQLCLGVLLLILPFVGGCDAMLDSGSERYTFEEDARLQTL
                     HDTLYNIVGIFSEMQKLGDRYVLLGELRADLMKVTDEASIDMKEIYYFNMSPDNAYAE
                     MKTYYSIINQCNYIIHYADTAYAEKSDKPLMRIYASAKSIRAWTYMQLVLNYGKVRFT
                     ELPILSVKEAEQDFPLIGMDALFTYLIDDLLPLKDIRLMEPGRFGNFDASRSIFPIPL
                     LLGDLYLWKGEYAAAAAMYYELIFEENLVINSQYIAEWGVENNAISTNSLSSAWFNSL
                     MLQNSEIVSALGAYPDYGHSFSLDSLSSQAKIMPTQIALDKWLNAVYYLNSSSTKNGD
                     LRIYDAVNNNYSMVKFGMITVDEADESVQELIKPTIRKFSQMNLSESEYDPRHLPLYR
                     SSHVYLRYAEAINRLGYPQLAFAVMKNGLKLETMSLPHLVAEMAAVDSVIPSFMSFTY
                     AFFDNNAGTRMRGSRNVHEDETYFIIPDSITLVQQGYVNYGTAPSKQDTIDYVEDILL
                     EEMAMELAFEGNRFHDLMRIAYRRGDNAYLADRVAAKYEASEQAAIHDMLMVQDNWFI
                     PQE"
     gene            13140..17387
                     /locus_tag="PKY68_00220"
     CDS             13140..17387
                     /locus_tag="PKY68_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOH23497.1"
                     /translation="MEEKKKCLTSLGLLIAFCLSSVCLSAADYHFRQLTSDEKLSQNF
                     VDDIYKDSKGFMWFATWGGLDRYDGYSVISYNTENAKLKSNFCYCLAEDSHERLWVGS
                     DLGLSCIDLNTGRSLNLIEENAQYRNALSQTVYDLHCDSQSKLWVAGDHGLVCLVFDE
                     QGGIVNLYSLNMLTVFALCEDYDGRIWLATSSGKLLCLSNKNPGQFDFEIVPELLRRE
                     TFDFIRSIYSDRTGSLWVASSQGLFRYDPHADILQGYQHSETKSGSLPNNFVNDVLQD
                     NTGNIWVATLGGLALWNQETGNFTRFQSDDTDESINNNFLNSLFVDDRNILWIGTEKG
                     GVNWMFEKRKAIYGLRHDPANPGSIYPGPVNSILEDSRGNLWVGLVEGGLHFRAKGQD
                     SFRHYLHNNKPGSLTQNTVSKIYEDSESTIWVGTWGGGLNRLRADGSGFKSYVPGDEK
                     GSIASLFISDLVEDSLHNGLWVGMNNGLQFLSKQSGQFLNVFVGSDIPYPPKQVNFLK
                     IDRRQRLWVATNFGLYCFYLHSFDIPGTPAKYDYFRFSLHDPSSGQLAKLISIYEDED
                     EDILWFGTNGDGLYKMEEISGADSADLKKKTGWEGQGVYKVAESYVRFVRYDKRHGFA
                     DNVIYTILEDDNDCLWMSTNNGLVRFEPQNTEVVNYSKEDGLLSNQFYWMAACKGHDG
                     TLYFGNLQGLNYIEPGRFKNNDYMPKPSLTSLQILNNTIEPGVAVNNKVYLEKHLYAS
                     SELVIPKKDKSFSLEFSALDYEFPECIRYSYYLEGFDKQWTDVGPERRFVYYTNLKQG
                     KYLFKLRCTNSDGVWSDKITGLPVVISPPFYQSLMFRLVLILVLLGLASFAFISREKR
                     IKQNRAELETKVKARTKELQEAYRQVEALNQEKLSVFTNITHEFKTPLSLIMGPIKKI
                     LNLSKDPLVLDQLKLAERNAENLLVLVNQLMDFRKVEEKASKLQKTRGNLLDLVREVL
                     LPFADAVERNFVQCSLISRLKNPELIFDAEILRKILSNLLSNAVKHTPDYGSIKIKLF
                     DIQKDNADWIRITVSDSGKGIPEEESEKIFERFYRIEDQETFRAAGQSGTGVGLFLCR
                     ELAQLHGGIIYAGNNRLGGARFVLEIPAEFPTSPVENLVKTENAVGDNVLDREEAEEG
                     RLRGNSSSAAAATPSKPLLLIVEDNKDMRQYLASVLREQYNLLEAADGEEGWTMVQRN
                     LPDMVISDVMMPRMDGIQLCRKIKTEFKTSHIPVILLTAFSSPNNEIEGLESGANDYL
                     SKPFEERLLKAKIKNILQLRERIHQSFAENMSTGQLEIAENSPDKYLLEKMMKIVQLR
                     YSDPLFNVSVFIEELGISRSLLHKKLQSLVGQSAGRFIRNYRLNKAKEIISIHPGMNI
                     SEVAYSVGFNDPKYFTRCFTKRYGVAPSVYSLNLHES"
     gene            17470..18258
                     /locus_tag="PKY68_00225"
     CDS             17470..18258
                     /locus_tag="PKY68_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH23498.1"
                     /translation="MSETGFCPELPVGSQDSTALPEKTKTTVVCNSGVSRYSNWYSFE
                     KPEALYARKFLTKSIAPVATAALGIGILGMSDFELQLQESLNWNKDLKVPMYDDQLRY
                     APIAIGVILPLFGKYPKHKFLHLIPLLSSAYLLADGVVHQLKAYTAIARPNGAREHDA
                     FPSQHSSMAFVAATLLHYEFGDYSPWISIGGYTLAGWVAYARVAQNHHWTSDVLTGAA
                     IGTLSTHLVYLSYNFLSDLFTKNNLTICPYPVEGGGGVYLSYSF"
     gene            18349..18900
                     /locus_tag="PKY68_00230"
     CDS             18349..18900
                     /locus_tag="PKY68_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOH23499.1"
                     /translation="MGKLDAYKIPLDSLSIGTHVFEFQLDTPWFELMDGPDVQKGKVK
                     AIVRVINTGRLYDLRFELTGVVEVPCDRCLDDVTIEVDQKSQLFVKLGPGFYEESEDM
                     IVIPEKEGALNVAWFLYELIVLGIPMRHVHAPGKCNKAMMSKLKEHLTGPGDEDEIEP
                     DLPDGEEFEAIDDDQALGSDWED"
     gene            18943..19125
                     /gene="rpmF"
                     /locus_tag="PKY68_00235"
     CDS             18943..19125
                     /gene="rpmF"
                     /locus_tag="PKY68_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOH23500.1"
                     /translation="MAHPKRKHSNTRTAKRRTHYKATLPTLAVCPNCGAQYIFHTVCG
                     ECGYYRGKLAIEQKES"
     gene            19261..20142
                     /gene="era"
                     /locus_tag="PKY68_00240"
     CDS             19261..20142
                     /gene="era"
                     /locus_tag="PKY68_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOH23501.1"
                     /translation="MHKSGFVNIVGNPNVGKSSLMNILVGEKMSIITSKAQTTRHRII
                     GIVNGPDYQIVFSDTPGVLKPKYKLQESMLNFSSSALVDADILLYMTDTVEKIEKNES
                     FLEKVKQATVPVLAIINKIDLSNEEALQKQVQALQAILPSAEVIPLSVLHKFGLDYLM
                     KRVLALLPESPPYFGKDALTDRPARFFVSEIIREKILLYYQQEIPYAVEVVVESYKEE
                     KEKIYIAALILVERESQKGIIIGHQGKALKKLGTTARREIERFTGKNVFLELFVKVEK
                     DWRTNENSLKNFGYIQD"
     gene            20153..21475
                     /gene="der"
                     /locus_tag="PKY68_00245"
     CDS             20153..21475
                     /gene="der"
                     /locus_tag="PKY68_00245"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOH23502.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRLVESRQAIVDEEAGTTRDRHYGF
                     AQWNDCEFSVIDTGGWVEGSDDVFEEAINRQVQLALDEADVILFLVDVAHGVTDLDQE
                     LASRLRRLNKQRPVLLVANKADNYEIGMNAAEFYKLGLGDPITVSAINGSGTGDLLDL
                     LVAKFDKTSPVERELEDLPRFAVVGRPNAGKSSLINSFIGEERHIVTDIAGTTRDSVY
                     TRFNKFGFDFYLVDTAGIRKKSKVTEDLEYYSVIRSIRSIESADVCILMIDATRGVES
                     QDMNIFSIIQKNKKGLVVCVNKWDLVENKSNETIKTFERAIKERFAPFVDFPILFISA
                     TTRQRIFKVLETADAVYKTRKQRIPTSQLNEYFLPLIEKFPPPANKGKYIKIKYVTQI
                     QGPYTPSFVFFANLPQYVKEPYKRFLENKLRSRWNFTGTPVQVFIRQK"
     gene            21497..21799
                     /locus_tag="PKY68_00250"
     CDS             21497..21799
                     /locus_tag="PKY68_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOH23503.1"
                     /translation="MEKKENQLNIELPEEVAQGTYSNLAIISHSSAEFILDFIRMMPG
                     VPKAKVKSRIILTPEHAKRLLMALQDNVVKYEKQFGPIKNPQAGSLPPLGFSTGEA"
     gene            21925..>22067
                     /locus_tag="PKY68_00255"
     CDS             21925..>22067
                     /locus_tag="PKY68_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOH23504.1"
                     /translation="MPTIQQLVRKGRVRQTESSKAPALDSCPQRRGVCVRVYTTTPKK
                     PNSA"
BASE COUNT         6228 a         4689 c         5113 g         6037 t
ORIGIN      
        1 caagaaaaca aaagaagaag ctctggagct tgccgaggct atggtaaccg gccagcggat
       61 gcacaccgac ggaagtggat ttacgattcc ggaaagagag atttctaccg ccatgcaaac
      121 cattaacgct aaagaatttg agggtttgca ggtaggtacc ctggacgaag ctttgcaggg
      181 gcgtgtagcc ggtctggata ttgtctccac ttcgggtaac cccggcagcg gttcatcgat
      241 gcgtatccgc ggaactgctt cgattaccgg taacaaggag cccctgattg tgcttaatgg
      301 tgtaccctac gaagttaata ttaaggattt tgacttcgcc acttccaacg aagaggacta
      361 tgcagagtta ttaagcataa atcccgacga tatagaacaa attactgtac tgaaagatgc
      421 ggcatctacg gctatctggg gatctaaagg cgccaatgga gttttgatgg tgacgaccaa
      481 aaaaggtcag gccgggccta ctttggtaca atacacctat cgttttaccc gttccgttca
      541 acccaaagga ttaaaaatgc tcaatgggga tgattattcc atgatgatca aacaagccta
      601 ttttaatccc aggcaggatg aatcggctgc cgatatcgat gaatttaagt attccgacac
      661 ttttgtagaa tacgaaaatt tcaataacaa taccgactgg atcaaggctg tttcgcagac
      721 gggtcgtact cacgaccact atctaaatgt gtcgggtgga ggagagcgtg ctacctttag
      781 ggtttcggga ggtttttacg atcagacggg tacgattatt gaacaacatc tcaaccgctt
      841 cacctccaag gcctatttgg attatgccgt atcggaacgc ctgaaattta tttcggactt
      901 ttcgtttact cacgtagata accagcggaa ctattccaac ctcctgggaa cagcctatgc
      961 caaaatgccc aatgtgagca tttatgaaca ggacagagac ggtaacgata ccgatgtatt
     1021 ctacaatata tcgcgcaact caagattgaa tgcggctcaa cgagacatga taaacccggt
     1081 agctattgcc aaactcgggg taaacaacct aaaaagttat cgtatcgttc ctatttttca
     1141 attacattac aatctgaccg gggagcgttc tcgttcgctt aggtatcaga tgtacgtctc
     1201 ttttgatata aataataata aaaactcggg ctttttaccc tgggaggcta ccaacaatac
     1261 ctggaacagc aaccaggtga accgctccga aagcaacgac tcggaatcac tttcggtatt
     1321 aactgaaaat gtcatcaact ggcagcctaa gcttagaaac gaagaccaca gcctgaccct
     1381 ctacggcgct ttgcaaacaa ggacgggaaa ttcaggcagt caatacattg ccagttatgg
     1441 tttgttttcg gataaaacca aagatgcctc caacgaagcc tatctggaag gcgtctcaac
     1501 cggacgatcc aattggcgtt caatgggttt ggtaggacgc gtgcattata cttacaagtc
     1561 gagatacatc atttcgggtg tggtcagaag agatggcagt acgaaattcg gaaataagcg
     1621 taaattcgga aacttcccca gtctctctct caaatggatc atttcggacg aggattttat
     1681 ggccgatacc cgctcctggc taagtatgtt ggccattcgc cccaactggg gtatgagcgg
     1741 gagagaaccc ggtgcagaat acttgcattt cagccgctac gccgcttacg acagttacct
     1801 gggtaagcag gcaacccgtc cggtcagcct gaggttgtcc gacttgaaat gggccactct
     1861 gagagaatgg aacattggag ccgatatagg gctctttaga gacaaaattg tcatggatat
     1921 gaactactat cacagacgta ccgtagacat gttgttctcc aatatttctc taccctctac
     1981 atcgggtttc gccagcatgt cttacgcaaa tgccggaacc atggacaacg acggctggga
     2041 attcaatgtt tatactaatc gcttggtcag aagaggagac ttcagtttgg atgtcaattt
     2101 caatatatcc aatcaggtaa ataccattat tgagttggat gaccggattc tggccaacta
     2161 caataaagat tacgacttcc gcaacggcac ctacatgacc cgtctgcagg aaggccactc
     2221 tttcggttcc atctacggat ttgagtacga gggtgtatat caatacgaca aatacgacga
     2281 agaacacgga catttggatg ctccggtagc cagggacaaa gacaacagag taattacaga
     2341 tgaaacgggt gaacccctgc ccatggtttt tgccgcaggt actacagtcg aatatgaatt
     2401 cagaggtggc gatgccaaat acaaagatca gaaccacgat ggtagtattg acgagttgga
     2461 cattgtatat ttgggtaatt gcaatcccct gtttaacggt ggtatgggat ttactttacg
     2521 ctataaaact ttgtcttgta ccgctttttt caactatcgt tatggcaata aagtagtaaa
     2581 tgctgctcgc atgaacgctg aaaatatgta cgataacgac aaccagagta ttgctgttaa
     2641 ctggcgctgg cgtaaggatg gtgatattac cgggattccc agagccttat acaattatgg
     2701 atacaactgg ctgggtagcg atcgttttgt agaagacgct tccttccttc gtttcaaata
     2761 cctgaccttt aactacattc ttccttcaaa ttggttaaaa cccataggta tcgatagagt
     2821 aagcgcttat cttacattta ataacttata tgtgtttact aagtattccg gtatggaccc
     2881 cgagattggt tacggtgatt tcggtataag caccgataac gcgcagactc ctcgttcaaa
     2941 agactttact ttgggtttga ccataggctt ttaaattatg aaaaagatga aaaacagact
     3001 ttttatcgtc tcgatattgg ccttgagcct tttcgcaaca acagcctgta ccaactggct
     3061 ggatacgcgt ccggaaagtg aaatgatttt ggatgaatac tggaccactg aatctgaagc
     3121 agaagccgtg ttgatggcag cctaccgtgg atttattact tcggattgca cctatcgtat
     3181 gttggtatgg ggggaattac gttcggacaa ttttacccag ggtaccggca ttggcgatga
     3241 aatcaataag gtactccggc aggaaatcac tccttccact ccttatacga cctggtcgtc
     3301 gttttattcc gtcataaatt actgcaacac ttttttgagt tttgctccgg atgtgcttaa
     3361 aaaagacgac aactttactg tggaaaagct ccgtaaactc agcgctgagg cttatgccgt
     3421 aagagcattg gcctatttct acctggtcag ggctttccag aatgttccct ggatcagcga
     3481 acccagtctg gacgatactc aggattttat agtgccgcag ttctccgaaa gagcggtact
     3541 ggactccatc attcgggatc ttgagctgta ttctctgcct tacattccgc ggatacattc
     3601 cacggataag gaaaccaagg gacgttttac caaaaacgga gtttatgcct tgctggcaga
     3661 tatctacttg tgggataatc aatttgagaa atgttcacag atgtgtgatt acattttggc
     3721 agacgactat tatacactgg tacctgccga aaatttttat tcccaggtgt tctactcagg
     3781 attttcttcc gaaagtattt ttgaattcgg ttacgataac aatattcagg tgaacgatgc
     3841 cacccgttca ttatatggtt accagaacaa ccagaatggc cagctggctt tccctgtcac
     3901 gctggtaagc ggacaataca gtcctttcga ataccctgcc ggaagctaca tagaaagtaa
     3961 aaacgacgtt gatattcgct ggaaagactt ttttgtgaac gatcccggtg tgggtgttta
     4021 tcaaatattc aagtatgccg gtattcagcg caccgaaaat ttcgacggat ccagaagcac
     4081 ttattcctat cgcaacaaca cctcgaactg gatagtgtat cgtttacccg atgttattct
     4141 gatgaaagca gaagcagtgc tgcaagggaa acaggaagaa ggcctggccg aggctctgtc
     4201 tctggtcaat atgacctatt cccgttccaa cgggggagat actttatcgc tcgattatta
     4261 cgataattct tacgataaac tggttgaact ggtattgaga gaaagacagc gagaattgat
     4321 gttcgaaggc aagcgctggt tcgacctgat gcgtatggcc cgcagggcag gcagccctgc
     4381 gcctctggtt tcgttcattg cccgtaaagg cgaaggcagc gaagccctgg gtaaaatgtc
     4441 acagatgaat gccctctatt ggccggtcaa caaaggagaa ctggaagcca atacggctct
     4501 ggtacaaaat aaattttatg aaacagaatc tacaacatcc actttgaaat aaaatagaca
     4561 gcatatgaaa acaaccaatg cctttaaaaa gaagatactt tatcttttgt tgcttttttc
     4621 catatctccg ggtttgtttc aatcctgtta taccgaagat gtggtagaca actatttcac
     4681 ctttacggga gaattgatcg gagactacat tgataatcgg cccgaacgtt tttcggagtt
     4741 taagcgtttg ctcgatacca cgggcgttat ggggctgttg aaagcctacg gcttgtatac
     4801 ttgttttctg cccaccaacg atgcgatgca aaaatactat caagatcacg aaaaaaacgg
     4861 ggccgaagat ttttccttag cagaactcaa gaccatttgt tataaccata ttattaaagg
     4921 agacaccctg gcaactatgc agttctctga aggtgctctg agttctatgt cgatgagcgg
     4981 tcgttttata agtgtcgatt tttccgatac aacggccacc atattcatga acgggacctc
     5041 tcctattata gaaaaagaca tcattttgca caacggagtg gtacatgtat tgagcaaggc
     5101 gatggaaccc tcccaagacc gggtttcttc caaattgatg catgatccga aattttcgct
     5161 ctttgccaaa gcttttgaat tgacaggtta cagatttatc atggacgagg ctcctataga
     5221 agatttaact tacgatccga aaaattatac tccaagcggt tttcgtcaca cagtgctgga
     5281 agaattaccg aaataccgga aattcggata caccatcttg gccgaaagcg actccactct
     5341 ggctaattat accgattgtc ccaaattgcc caacggtgta aaaacgctgg aagatttgta
     5401 tactctggct gaatactttt attccagcaa gccggggaac ataggtctgt tttccgacgg
     5461 ggacgaggtg aaagacgatt ataccgacaa aagaaattac ctgaacaggt atatcgctta
     5521 ccactgtatg acccgcaccc tgctttcgag ccgttttatc aaagattacg acactccgca
     5581 tcatttcaaa atctacgaca tgtacgaata cattgagact atgttggagg accgtttgct
     5641 tgaggtaaag atagaccgta attacagcgc ccccaacagc gagttcggtt ttttcaactg
     5701 taaaatggct gccgacggca aagaagatgc ttctaccggc attttgttga ccgattacaa
     5761 agataatgac gctgtaaacg ggtattatca cgaaattgac aaacctctgg cttacaccag
     5821 agagtttgag gccgctcttt ccgcgaaacg gcttcgcatt gaagctgcct gtttctttga
     5881 tgaactggct accaacaaca tgaggggaaa tgccaaatgg aaccccaatg gaagtaacca
     5941 cggagaaccc gataaaaccc atgcttatca gattcctttt ggctacatgg accgtttttt
     6001 ttgcaccgaa agcaccaagt ttacatatat tggagcctgt tcagcttacg aggattttca
     6061 gggagacgaa atgtacgctc gcgattctta caatttcact atgcagactt tgcccatacc
     6121 tgccggaact tacgaagtgc gaatgggtta ccagcctacg gcttaccggg gtattgcaca
     6181 ggtgtactgg gatagtattc ccacgggcat tcctgtgaac ttttctttat tggccagtaa
     6241 tcccgatata ggacataaag ttccgggcag tgaagcggat gatccttatg gctatgaaaa
     6301 cgacaagatg atgcgaaatc gtggctacat gaaaggcccc gacagttata aatgtccggt
     6361 caacatctat tcttatagtg ccgctaatgc acgttacagt acctataacc tgcgttacat
     6421 cctgggaact tacgaattta aagagccggc taaacactac attaccattg tctttttggg
     6481 aacggaatcg ggcgatagtc aatttatgat ggattacctg gaattttgcc ccactgaatt
     6541 gatacttaca gaagatacac attaaacata aatcatatga agaccaacca ttcgaaatta
     6601 tggcccttga ttgtgctatt cctgttcgcg ctcatatctt gccaggaaca agcctggtat
     6661 gagcactatg aagattcggg aaaagccgga tctgaaaaga ccatgatgga atatataaaa
     6721 tcggaaccgc aactgggtct gtttttacaa atgctggagg tttcgggtta cgatacgttg
     6781 ttgtccgttt ctcaaacttt tacggtatgg gctccccaaa acaatgcctt gtcggggata
     6841 gacctcgacg ataccaccac cgttaccgaa ataatcagta accatatagc ccgctactgc
     6901 ataggtactt cttctaccag gagcgataag gtgtttatgc tcagcaataa actggtttct
     6961 tttgaaggta acaagtttgg tttaacaaat ttggtcagct ccaatataaa agtagccaac
     7021 ggcctcttgc acataatcga cggttttgtt ccttatgtgc cgaatatctg ggagtataca
     7081 ggtcgcattc cgggtttgga ctctctggct gcgtatttcc tcagtcagaa tcaacgagag
     7141 tttaatctgc tggaaagtaa acagatagga gtcaatgcag ataatcagcc tatttacgac
     7201 tcttctttta ttgaaagcaa ccgctattta accgagcggg ttaacctgaa caacgaagac
     7261 gctgttttta ctgcactttt ccccaacaac caagcctggc ataagtcttt tgatctcatt
     7321 aaggattatt acaaaactac cgatgccgac gggggttgga ctaaacaaaa atcgctggct
     7381 caggcagctt tagtcaaaga cttggtttac cttgataatt tcaaagaaaa gggaattccg
     7441 gactctcttg tgacttcgtc gggccatgta atagacagtg ccaaagtttt atttcagggt
     7501 gcagttagcc acgacgccag caatggggta atttatgtta cggattccct gagaataccc
     7561 gccaccagct cctggcatat tcctttccag atagaggccg aagacaacta ttacggccgg
     7621 acttctctca atgccaagtt gtataatcga accagcgccg gtacttctgt gtcggcatcg
     7681 ggcggcaggt atctggaagt ggaggccacc ggcacaacca tcttgtcgaa aacctatgtg
     7741 gattttccca taccggacgt tttgtcggca aaatacgata tatactgcct gtttgtgccc
     7801 gaaagcgttg tgaatcgcat caatccgaaa cccaatgtgg tcaatttgta tatgtcgtat
     7861 ttagggccag acggaaagtt gaaaaacaat gtttcgataa agaaaaatgt gacaatcgat
     7921 gcagaagata ccacccttgt acatgtgaca cagatggatt tcccctattg cagcgtactg
     7981 gatcgcaatt ccgaaataag cgatttaact gttaaactca gggttgagaa tgcggttact
     8041 gccagtcaga cgcgattgta tacaagaacg atgcgcatag attgtataat ttttataccg
     8101 gtcgaagact aaacctgtaa aagcacgaat gtattatgaa aagaaatagc agttacatga
     8161 gcaggatcat tttatcactg ggtcttttgt gggtcgcagt aactttaggt tcagtaaaag
     8221 cagcagataa agctgcagag agcaagctct tcgtgaaagg ccaggtatta agccaggcta
     8281 ccggtagtcc cgttttcggc gctactgtca gagccggaga atcgtctgtg tttactaacg
     8341 aagaggggcg ttttgagttg attattcaaa cagccaacga tgttttggtt gttaccggag
     8401 acgatttcat tgcccgcgaa tatcctctca gaggcaggca ggacgttgtc atagagcttt
     8461 acccccgaat atttggcgaa aactataaaa cagtggagct tcccggagga aaaactaaaa
     8521 gctcgaagat gatttcttcg gccaagacag ttgatatagg gttgaatcaa gcggtttcag
     8581 cggatgaact ctaccattcc tcattgggag gagacatacg taccatcagt cgttccggtt
     8641 tgcccggcat aggaagcctg ctttttatcc gtggtttgaa ttctctgaat gccaaggcac
     8701 atccgttgtt ggttgttgac ggtgtaatat ggaacgatat ttccgatgtt caatccattt
     8761 ttgaaggctt tcaatccaat attttgatga atattgatgt gaacgacatt cagagcatca
     8821 cggtattgaa agacggtacg tctctctacg ggagcaaggc tgccaacgga gtcgttagta
     8881 taaaaaccaa gcggggtgag agtgctgtta ctaaaattga ccttcgcatt atggccggca
     8941 tggttgccga acccggaaat attccgatga tgaacgccga tcaataccgg gtttatgcca
     9001 gcgagttgct gcaaaccgtc aatccgggta tgttgaaaac acccaaattt ctggattttt
     9061 tgatcgaaaa tccttccgat cccatctaca atacctatca taatgagacg gattggtcgc
     9121 aagaagtgta taagcgaggc atcacgcagg gttacaacat cagtgtgaac ggtggcgacc
     9181 aacgggcact ttatcatttt tccctgggtt ataccagcaa taagggtata gtcaagacaa
     9241 cggaaatgga gagaataagt tcccggttca atgccgatat caacgtactg gacaatctaa
     9301 acgttggcat gaacgtcggt ttttcaaata taatgcgcag cctgttggac gatggagtta
     9361 acttttatac ttcgcccact tacatgggct atgccaaatc tcctttctta agtccttatt
     9421 cctttacttc cttgggctac aaagcaaagg attacgacga tgcagactta tttggtatag
     9481 gaaatccctc ttcatttatt gcaggttctc aaaacaaata caaagagtac cgctttaatg
     9541 tcggctttat gccggtatat aacatcaaca aacattggac tttgagtacg ctttttgatt
     9601 acagcctcaa caaatacctg gaggactatt tcagtcccat gcacggttct tatccccgtg
     9661 agcttgaagg ttttggtatg tccgaaaaca ccgtcaagaa tcagttgatc cgcaatacgg
     9721 ccatttttga cgatacccgc ttgagttacc atctactcaa aaagaatttt gaattcaaaa
     9781 ctttgctggg ctggcgttat atgaccaatt atctcgaagc cgactatatt gaatcgcata
     9841 attcgggtac agataataat actaccgtag ggaattacga ttttcttcag acccacggtc
     9901 tgaataatcg tacaaaatcg gtttcgactt atgccaacgt tgatttggca ttcctgaaca
     9961 ggtattttat caatgccgta ctctcgctgg acggatcttc gcgtttcggc aaagaggcca
    10021 aaggaggtgt tgagctcttt ggtcacagtt ttgccctgtt cccggcattt aacgcggctt
    10081 ggttgatttc gtccgaatcg tttatgaaaa acatccggtt catcgattat cttaaattgc
    10141 gcgcgggcta cgattttacc ggtaacgatg gcattcagga ttatgctacc aaggcttatt
    10201 ttgttaccag acgctatata ggccaggcta atggtgttat tcttagcaat ttggcaaacg
    10261 aggaactgca gtgggaaacc actcaaagca tgcatgccgg tttggatatg actttgttta
    10321 acgaacgttt gctgctgaat gtggacgtgt tcgattcaaa tacggataat ttgttaacac
    10381 tcagaagcct gaatcagatt gccggtgtcg attattactg gagcaacgaa ggtaaacttt
    10441 caaacaaggg ttttgaaata tcagccgaaa ccaaattggt aaatactaaa aacttccagt
    10501 ggagccttgg agccagcata gggcattacc gcaatttagt tgtagagttg cccgaaaaag
    10561 gctatactac tcctgcttat ggcggcgaaa tcagaaccga ggtcggacag cccgccggag
    10621 ttttctgggg ttacaaaact cagggcgttt acgcttccga agccgatgct ttggccaatc
    10681 ccatggctat cgtaaacgac aacggttcac tgacctattt taaagccgga gacgtaatct
    10741 ttgaggattt taaaaaggac ggcatattgg acgaaaacga caaacaggtc atcggagatc
    10801 ctaaccccga tttctacggc aatttttcga ctaaactcag ttttaagaaa tggaccttaa
    10861 ataccttgtt tacttattct tatggcaacg atgtttataa ttacctgaga agccagttgg
    10921 aatcgggtaa ggattttagc aaccagacca aagctatgtt gttgcgctgg acggccgaag
    10981 gacagaacgc cactttgccg agaagcgtat acgacgatcc tatggggaat gcccgttttt
    11041 cggatcgctg gatagaagac ggttcgtttt tgcggcttaa atccgtttca ctttcttata
    11101 ccattcccat tgagaatgtg tttatcaaag gccttgaatt ctcgctttct gccaacaacc
    11161 tgtttttgtt caccaattac ctgggtctgg atcccgaaag ttcggtcagc gacaaggtat
    11221 tgtttcaggg catagataac ggtttgattc ctcaaacacg aagttacttt ttgggagtca
    11281 aggtaaatct ctaattacta tcactcatat taatatgaca agaaaaatga aagctttcaa
    11341 acaattatgc ctgggtgtgt tgctgttgat cctgcccttt gtgggcggtt gcgatgccat
    11401 gttggattcc ggttccgaaa gatatacttt cgaggaggat gcccgtttgc aaaccttaca
    11461 cgacacactt tacaatatag tcggcatttt ttcggaaatg caaaaactgg gcgatcgcta
    11521 tgtattgcta ggagaactgc gtgccgacct gatgaaagtg acggatgagg cctcgatcga
    11581 tatgaaagaa atttattatt tcaacatgtc gcccgataat gcctatgcgg aaatgaaaac
    11641 ctattattcc attattaatc agtgcaacta tatcatacac tacgccgata ctgcctatgc
    11701 tgaaaaaagc gataagccct tgatgcgtat ttatgcttcg gccaagtcta tacgagcctg
    11761 gacttatatg caactggttt taaattatgg taaggtgcgg tttactgaat tgcctattct
    11821 ttcggttaag gaggccgaac aagatttccc gttgattgga atggatgctc ttttcactta
    11881 tttgatagac gatttgctgc ctctcaaaga cattcgcctg atggaacccg gtaggttcgg
    11941 aaattttgat gcttcgcgct ccatctttcc catccccctg ttgctgggtg atctgtatct
    12001 ttggaaagga gagtatgcgg cagcggcagc catgtattat gagttgattt ttgaagagaa
    12061 tttagttatt aattctcagt acattgctga gtggggagtg gaaaacaacg caataagtac
    12121 caattcactt tcatccgctt ggtttaactc gctcatgttg cagaacagtg aaatagtttc
    12181 ggccctaggg gcttatcccg attacggaca tagtttttcc ctggattccc tgagctcaca
    12241 ggctaaaatt atgccgacac agatagcact cgacaagtgg ctcaatgccg tgtattacct
    12301 gaactcgtct tctaccaaaa atggggattt gcgcatttac gatgctgtca ataacaatta
    12361 ttccatggtt aaatttggta tgattactgt tgacgaggcg gacgaatctg tacaggaatt
    12421 gataaagcct actatacgga aattttcgca gatgaatttg tccgaatcgg aatacgatcc
    12481 cagacatctg cctctgtacc gtagttcgca cgtgtatttg cgttatgccg aagctataaa
    12541 ccgcctgggt tatccccaac tggcttttgc cgttatgaaa aacggcctga aactggaaac
    12601 catgagtctg ccacacttag tggccgaaat ggctgcagtc gatagtgtta taccttcatt
    12661 tatgtcgttt acctatgcct ttttcgataa taacgccggt acacgaatga ggggcagtcg
    12721 caatgtgcac gaagacgaaa cttattttat tattcccgac tccatcactc tggtacaaca
    12781 gggctacgtc aattacggaa cggctccttc caaacaagat accatcgact acgttgaaga
    12841 catattgctg gaggaaatgg ctatggaact ggccttcgaa ggaaatcgtt ttcatgacct
    12901 gatgcgtatt gcttaccgga gaggcgacaa tgcctatctg gccgaccggg tggctgctaa
    12961 atacgaagct tcggagcaag cagcaatcca tgacatgctg atggttcagg ataattggtt
    13021 tatcccgcag gagtgacggt ttgcatttat agagggagga atctattaag tcctacttag
    13081 gctatctggc ttcttttttg tatcattgtt gcgattttgt ctatcgaaag acattttgca
    13141 tggaagaaaa gaagaaatgc ttgacaagcc tgggtctgct tatcgcattt tgcttaagct
    13201 ctgtatgttt atcggcggct gattatcatt tcagacaatt gacctccgac gagaagctgt
    13261 ctcagaactt cgttgatgat atctataaag acagcaaggg ctttatgtgg tttgcaacct
    13321 ggggaggttt ggatcgttac gacggttact ccgttataag ctacaacacc gaaaacgcaa
    13381 aacttaaaag taatttctgt tactgtttgg ccgaagacag tcatgagcgg ctctgggtgg
    13441 gatcggattt gggcttgtcc tgtattgatt tgaacaccgg ccggtcattg aacttaatag
    13501 aagaaaatgc gcaataccgg aatgctcttt cccaaacggt ttacgatctg cactgtgatt
    13561 cacaatctaa gctctgggtg gccggagatc acggcctggt ttgcctggta tttgatgaac
    13621 agggcggcat cgttaatcta tacagtttaa atatgttgac agtcttcgct ctgtgtgaag
    13681 attacgatgg ccgtatctgg ctggctactt cttcagggaa gttactatgc ctctccaaca
    13741 agaatcccgg ccaatttgat ttcgaaattg taccggaatt actacggcgc gaaacatttg
    13801 actttattcg cagtatctat tccgacagaa cgggctctct ctgggttgcc agttcccagg
    13861 gcttgtttcg ttacgatcct cacgctgata tcctgcaagg ctaccaacat tcggagacaa
    13921 aatccggatc tttacctaat aattttgtaa acgacgtttt gcaggataat accggcaata
    13981 tctgggtagc aactctgggc ggtctggctt tgtggaatca ggaaaccggc aattttaccc
    14041 gttttcagtc ggacgacacg gacgaaagca taaacaacaa ttttctgaat tctctttttg
    14101 tagacgatcg taacattttg tggattggaa ctgaaaaggg tggagttaac tggatgtttg
    14161 aaaagcgaaa ggcaatatat ggcctccgtc acgatcctgc caatcccgga agtatttatc
    14221 ccgggcccgt caattccata ctcgaagaca gccggggcaa tctttgggtg ggcctggtcg
    14281 aaggagggct gcatttcaga gccaagggcc aggattcttt tcggcattac ctgcataaca
    14341 acaagccggg cagccttacg caaaatacgg tatccaaaat ttacgaggat tccgaatcca
    14401 ccatatgggt gggaacttgg ggagggggac tgaatcgcct gcgtgccgat ggttccggtt
    14461 tcaaatcata tgtgccggga gacgaaaaag gcagtattgc cagccttttt atttcggacc
    14521 tggtcgaaga cagcctgcac aatggtttat gggtgggcat gaataacggt ttgcagtttt
    14581 tatctaaaca aagcggacaa tttctgaatg tatttgttgg ctcagacatt ccctatccgc
    14641 ccaaacaggt aaattttctg aaaatcgatc gtaggcagcg tttgtgggtg gctaccaact
    14701 ttggcttgta ttgtttttat cttcactctt tcgatattcc cggaacgccg gcaaaatacg
    14761 actatttcag atttagttta cacgatcctt cctcggggca attggccaaa ctaatcagta
    14821 tctacgaaga cgaagatgaa gacatcttgt ggtttggaac taacggcgac ggcctgtata
    14881 aaatggaaga aataagtgga gcagacagtg cggacctaaa gaaaaaaacc ggctgggaag
    14941 gtcagggtgt gtataaggtt gcagaatctt atgtccgttt tgtccgttac gataaaagac
    15001 atggttttgc cgacaatgta atctatacca tactcgaaga cgacaacgat tgtttatgga
    15061 tgagtaccaa taatggattg gtccgctttg agcctcagaa taccgaggtg gtcaattatt
    15121 ccaaagagga tggcttgttg agtaatcaat tctactggat ggcggcttgt aagggtcatg
    15181 atggaacact gtattttggt aatttacaag gtttgaatta catagaaccc ggacgcttca
    15241 aaaacaacga ctacatgcct aaacccagct tgaccagctt acaaattctc aacaatacaa
    15301 tagagccggg cgtggctgtc aacaacaaag tttatctgga aaaacacttg tatgcaagct
    15361 ctgagttggt gataccaaaa aaagataaat ctttttcgct ggagtttagc gctctggatt
    15421 atgagtttcc tgaatgtatc cgttattcct attacctgga gggttttgac aaacaatgga
    15481 ccgatgtggg gccggaacgt cgttttgtct attataccaa tcttaaacaa ggcaaatatc
    15541 tgtttaagct caggtgcacc aacagcgacg gagtatggtc ggacaaaatc accggtctgc
    15601 ctgttgtaat aagtcctccc ttctatcaga gcctaatgtt tcgcttggta ctgatccttg
    15661 tgttacttgg attggcttcg tttgcgttta taagcagaga aaagaggatc aaacaaaatc
    15721 gggcagaact cgaaaccaag gttaaagcta gaactaaaga acttcaggaa gcttaccggc
    15781 aggtggaggc tctgaaccaa gagaagttat cggtgtttac caacattact catgagttta
    15841 agacgccttt aagtttgatt atggggccta taaaaaagat actcaacctg agcaaggatc
    15901 ctctggtact ggatcagttg aagttggcag agcgcaatgc cgaaaatttg ctggtgctgg
    15961 tcaatcaact gatggatttt agaaaggtag aggaaaaagc ctctaaattg cagaaaaccc
    16021 ggggaaattt gctggatttg gtccgggagg tgttattacc ttttgccgac gctgtcgaga
    16081 ggaacttcgt tcagtgttcg ctgatatcca ggcttaaaaa tccggagctt atttttgatg
    16141 ccgagattct gcgtaaaatc ctgagtaatc tactgtcgaa tgcggtgaaa catactcccg
    16201 actacggcag tatcaaaata aagctttttg atatacaaaa agataatgcc gactggataa
    16261 gaatcacggt cagcgacagc ggtaaaggca ttcccgaaga agaatcggag aaaatattcg
    16321 aacgttttta tcggatcgaa gaccaggaaa ctttccgggc cgctggacaa tccggaacag
    16381 gggtggggct cttcctgtgc agagaactgg ctcagttgca cgggggtatc atttatgccg
    16441 gcaacaatcg cttgggcggg gcccgttttg tgttggaaat tccagcagaa tttccaacaa
    16501 gtccggttga aaacttagta aagacagaaa atgcagttgg tgacaacgtt ctcgaccggg
    16561 aggaggcaga ggaggggcgc cttagaggaa acagctcatc cgctgctgct gcgactccat
    16621 cgaagcctct gttgttgata gtggaagata acaaagatat gcgtcagtac ctggcttctg
    16681 ttttacggga acagtataac ttgttggaag ccgcagatgg cgaggagggc tggacgatgg
    16741 tgcagcgtaa tttgccggac atggttataa gcgatgttat gatgcctcgt atggacggca
    16801 tacaattgtg tcgcaaaatt aaaacagagt ttaagacatc tcatataccc gtcatattat
    16861 taacggcttt tagctctccg aacaatgaga tagaaggttt ggaatcggga gccaacgatt
    16921 acttgtccaa gccttttgaa gaacgtctgc ttaaggcgaa aataaagaat atcctacaac
    16981 ttcgcgaacg cattcaccaa agttttgccg agaatatgag caccggccag ttggaaattg
    17041 ccgaaaattc ccccgataaa tatttgctgg aaaaaatgat gaaaatagtt cagttgcgct
    17101 attccgatcc tttgtttaat gtaagtgttt ttatagagga attgggtatt tcccgcagtt
    17161 tgcttcacaa aaaattacag tccttagttg gccaatcggc cggacgtttc atcaggaatt
    17221 accgattgaa caaggctaaa gaaatcatca gtattcatcc gggcatgaat atatcggagg
    17281 tggcttattc cgtgggattc aacgatccaa agtatttcac ccgctgtttt accaaacgtt
    17341 acggagtggc tcccagtgtt tattccttga atttgcatga aagctaaagc gattttgctg
    17401 tttacttgcc tgttgtgttt atcgcatttt tgctttgcgc acattgtcgt tgatgaaact
    17461 gacagcccgt tgtccgagac gggtttttgt ccggaattgc cggtaggctc acaggacagt
    17521 acagcgttgc ccgaaaaaac taaaacgact gtggtttgca attcaggtgt gagtcgatac
    17581 agtaactggt attcctttga aaagccggag gctttgtacg ccaggaaatt tttgaccaaa
    17641 agcattgctc ctgtagcgac ggcagcgctt gggattggaa tactcggaat gagtgatttc
    17701 gagttgcaac tgcaggaaag cctgaactgg aacaaagact tgaaagtacc catgtatgac
    17761 gaccagttac gctatgcacc catagccatc ggagttattc tccctttgtt cggtaaatac
    17821 ccaaagcata aattcttgca tctgattcca ttgctgagtt ctgcctattt gctggccgac
    17881 ggagtagtac atcaactgaa ggcatatacg gcaatagcac gtcctaatgg tgcccgggaa
    17941 cacgatgctt ttccatcgca gcacagttcc atggcctttg tggctgctac gctactgcat
    18001 tatgaatttg gagattacag tccatggatt tccattggag gttataccct ggccggctgg
    18061 gtggcatatg caagagtggc tcaaaatcac cattggacga gcgatgtgct cacaggcgcc
    18121 gccatcggca cgctcagcac acacttagtc tatctgagct acaacttcct ttcggatctt
    18181 tttaccaaaa acaatctgac aatctgtcct tatccagtcg aaggcggagg cggtgtttat
    18241 ctgtcttatt cattttaagt cgaatccgaa gagaacaaaa attcatataa atattgattt
    18301 gtctgatttt ttttctacct ttgcgcggtt ttttagtaag tattgtaggt gggtaagctc
    18361 gatgcatata agattccgtt ggacagcctg agcattggaa ctcatgtttt tgaatttcaa
    18421 ctggatactc cctggttcga attaatggac ggaccggatg tgcaaaaagg caaagtgaaa
    18481 gccatagttc gggtaatcaa cacgggccgc ttgtatgatt tgcgttttga gctgaccggt
    18541 gtggtagagg taccctgcga cagatgcctg gacgatgtga ctatagaagt ggatcaaaaa
    18601 agccagcttt ttgtcaaact cggcccgggg ttttacgaag aaagcgaaga catgatcgtt
    18661 attcccgaaa aggaaggagc actcaacgtt gcatggtttc tatacgaatt gatagttctc
    18721 ggtataccta tgaggcatgt gcatgctccg ggcaaatgca acaaggcaat gatgagtaaa
    18781 ctcaaggaac atctgaccgg tcccggcgac gaagacgaga tcgaaccgga tttgcccgac
    18841 ggggaagaat ttgaagccat agacgacgat caggctttgg gatctgactg ggaagactag
    18901 aaaatggaat acaaataata ctaatttata atatgtactc aaatggcaca ccctaagcgc
    18961 aaacattcga ataccagaac tgccaaaagg agaacgcatt acaaggcaac acttcccaca
    19021 ttggctgttt gtcccaattg tggcgcccag tatattttcc atacggtttg tggagaatgc
    19081 ggatattatc gcgggaaatt ggcaattgaa caaaaagagt cctgattgaa ggtttttttc
    19141 gggaacgatt tcttcccccg gtagtgatac cgcggccgaa gacctggtat aacggatgtc
    19201 acaatatata tggtgttacg tatataaagt gaatgtccgt tttttcattt atatcctaat
    19261 atgcataaat ccggttttgt aaatattgtc gggaatccta atgtagggaa gtcgagcctg
    19321 atgaatatcc tggtggggga aaagatgtcc attattacat ccaaagctca aaccacccgc
    19381 cacaggataa tcggaatcgt caacgggccg gattatcaga ttgttttctc cgataccccc
    19441 ggcgttctga agcctaaata caaattacag gaaagcatgc tgaatttttc ttcgtcggct
    19501 ctggtagacg cggatatact tttgtatatg accgataccg tcgagaagat cgaaaaaaac
    19561 gaaagttttc tcgaaaaagt aaaacaggca acggtacctg tgctggctat aattaataaa
    19621 atagatctga gcaacgaaga agccttacaa aaacaagttc aagctttgca ggctattttg
    19681 ccaagtgccg aagtgattcc cttatcggtg ttgcataaat tcggtttgga ctatttaatg
    19741 aaacgtgtat tggctttatt gcccgaatcg ccgccctatt tcggcaaaga tgccctgaca
    19801 gaccggccgg cgcgtttttt cgtgtcggaa atcatacgcg aaaaaattct gctttattat
    19861 caacaagaaa ttccctacgc cgtagaggtg gtagtggaaa gttataaaga agagaaagaa
    19921 aaaatataca ttgcagccct gattcttgtt gagcgggaat cacaaaaagg gattataatc
    19981 ggtcatcagg gcaaagccct gaaaaaattg gggactacgg ctcgccggga gatagaacgt
    20041 tttacgggga aaaatgtatt tcttgaattg tttgttaagg tcgaaaaaga ctggcgtaca
    20101 aatgagaaca gtttgaaaaa cttcggatac atccaggatt aatgacagta atatgggaaa
    20161 tatagtagcc atagtaggcc gtcccaacgt ggggaaatcg actttgttta atcgtttggt
    20221 cgaaagccgt caggccatag ttgacgaaga agccggcacc acccgtgacc ggcattatgg
    20281 atttgcccaa tggaacgatt gtgagttttc ggtaatagat accggcggct gggtagaagg
    20341 ctcggacgat gtgtttgagg aggctatcaa cagacaagtg caactggcct tagacgaagc
    20401 cgacgttatt ttgtttttgg ttgacgtcgc ccatggtgtc accgatctgg accaagagct
    20461 ggcatcccgc ttacgccgcc taaacaaaca acggcccgtt ctgctggttg ccaacaaagc
    20521 cgataattac gagattggta tgaatgccgc cgagttttat aaactggggc tgggcgatcc
    20581 tattaccgtt tcggccatca acggttcagg aaccggcgac ttactggatc tgctcgttgc
    20641 aaaattcgac aaaacctctc ctgtggaaag ggaattggaa gatttacccc gttttgccgt
    20701 ggtgggccgt cccaatgccg gaaaatcttc cctaatcaat tcttttatag gagaggagag
    20761 gcatattgtg accgatattg ccggcaccac ccgcgattcg gtgtatactc gttttaataa
    20821 attcggattt gatttttatc tggtagatac tgccggaatc cgcaaaaaaa gcaaagtaac
    20881 cgaagatctg gaatactatt cggtgatacg ctctatacgt tccatcgaaa gcgccgatgt
    20941 ttgtatcttg atgattgacg ccaccagagg tgtcgaaagc caggatatga atattttttc
    21001 catcattcag aaaaacaaaa agggcctggt ggtttgcgtc aacaagtggg atttagtcga
    21061 aaacaaaagc aatgaaacca tcaaaacttt tgagagggcc atcaaagagc gttttgctcc
    21121 ttttgtggat tttcccattt tgtttatttc ggccacgacc aggcagagaa tatttaaagt
    21181 gctcgaaact gccgatgctg tgtataaaac aagaaaacaa cgtattccga ccagtcagct
    21241 taatgaatac tttttgcctc tgattgagaa atttcctccg ccggccaata aaggtaaata
    21301 cattaaaatt aaatacgtaa ctcaaatcca ggggccttac actcccagtt ttgtattttt
    21361 tgccaacctg ccccaatacg tcaaagagcc ctacaaacgt tttcttgaga acaaactccg
    21421 ttcccgctgg aattttacgg gcactcccgt gcaggtgttt atccgtcaaa aataatttac
    21481 ataaatctat attctcatgg aaaaaaaaga aaatcaactc aacattgaat tacccgagga
    21541 agtcgctcag ggaacttatt ccaacctggc cattatttcg cactccagcg cagaatttat
    21601 tttggatttt atccgtatga tgcccggagt tcccaaggcc aaggtgaaat ctcgtatcat
    21661 tctaactcct gaacatgcca agcgtttgtt gatggctttg caggataacg tggttaagta
    21721 cgaaaagcaa ttcggtccca ttaaaaatcc tcaggcaggc tccctgcccc cgctgggttt
    21781 cagtactggt gaggcttaaa aaatcttgtc gacttatttg tataaaataa aaagactttc
    21841 tatctttgca gcccgttttg agtaaaaaaa tgggcacata tttaaggatt aacgatttaa
    21901 ggatttaaga ccaaaagaag tagtatgccg acaattcaac aattagtaag gaaaggaagg
    21961 gtaagacaga ccgaaagcag taaggcacct gctctggatt cctgtcccca aaggcgtggc
    22021 gtttgtgtac gtgtctatac aactacaccc aaaaagccga attctgc
//
LOCUS       DAOSGL010000004        19395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_2280_length_19395_cov_4.756618, whole genome shotgun sequence.
ACCESSION   DAOSGL010000004 DAOSGL010000000
VERSION     DAOSGL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19395
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2280_length_19395_cov_4.756618"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="PKY68_00260"
     CDS             complement(<1..1665)
                     /locus_tag="PKY68_00260"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOH23505.1"
                     /translation="MKSKIYLILISSILLSACAGAGKRYVLKSPSGEIGVSVTDSGLM
                     VSYEKRPIQFIKMDITPVGKNAVALKTGDTPLEYHMLSGKRSDCSNVFRQMLLRCEGL
                     VLDLRVFNDGVVLRKITDGTDPEISYIIPDGQKRWSQNLRTDYEGFYPMRTEARSGSY
                     GYPLLTEYADGLFGLISESDVQHGMSGSHLEVRDDNAYHIKYMSEGKSENGISPAFVL
                     IIGSLADIVESTLVTDVATPCQIKDISWIHPGLSSWIYWAYNRSSKDFGKVKEYIDLA
                     AEMSWPYTLIDWEWDEMAGGGDIEDAMAYAREKGIKVNLWYNSGTSWVGPGAPTPLDR
                     LRTAEARESEMTRLNNMGVSGIKVDFFLPDGADMIDYYLDILEDAARHRLLVNFHGCT
                     VPRGWNRTWPNLMTMEGVYGAEWYNNTPRMTKAAASHNATLPFTRNVIGPMDYTPCTF
                     SDSQHKHITTDCHELALPILFESGLQHMADRPESYLSLPYQVKDLLMGLPSVWDDTRL
                     LAGYPGESAVIARRSGDRWYIAGINGTDEVLELTPDFSRLEQQGKVT"
     gene            complement(1785..1928)
                     /locus_tag="PKY68_00265"
     CDS             complement(1785..1928)
                     /locus_tag="PKY68_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23506.1"
                     /translation="MCYYVLNTYDENVHLTRVDDQEGRLVLVLYSNRGWWYHLSTPVC
                     EDK"
     gene            complement(2167..2454)
                     /locus_tag="PKY68_00270"
     CDS             complement(2167..2454)
                     /locus_tag="PKY68_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23507.1"
                     /translation="MNIKQLKSKIKTQLKKEKYPISLKHPWDLLKFRYANYFEFMLPG
                     ESYCLEEKNNKWEVFYTERGSKFDIKEFSSETEACEYFYNWIMQRNSESQQ"
     gene            complement(2454..3272)
                     /locus_tag="PKY68_00275"
     CDS             complement(2454..3272)
                     /locus_tag="PKY68_00275"
                     /inference="COORDINATES: protein motif:HMM:NF025387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TNT domain-containing protein"
                     /protein_id="HOH23508.1"
                     /translation="MVGAGAGAATAATGGAAGAGFWATAGVAAAGSAATMGTNSIIAQ
                     TGHNFSGMGNVDWGQVGISSAIGSVSGFAGSAAGYWAANSSMLVSGINSPILRSAIVS
                     PLAAGAGHIAGGTTAGLLQGQSFNTAFENSFKGIGNSMLMGAALGVTTTVGMSFAKGV
                     NPITGKTMWPKNNGFNGSPERTILKGGTTFDRYGDETGRFAAPDGTPFDSRSLPPNAK
                     LSPLTTYKVVNPLPAYQGTSSGSFWFNSSGGGTQYMFDYNINYLLQNSYIIRVS"
     gene            complement(3291..3887)
                     /locus_tag="PKY68_00280"
     CDS             complement(3291..3887)
                     /locus_tag="PKY68_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOH23509.1"
                     /translation="MDDQRRKSVFTQGSATTTRYYLGDYEEVYKPDGTIQKIHYLSGG
                     SMMLQQGGTKSLYYGYHDYQGSLIAWTNQSGTVVERRAYDPWGRHRNPQNWTEVDYTS
                     PSQLNNRGYTGHEHLDAFQIINMNGRVYDPLTAQFFSPDPFVQAPDNWLNYNRYAYCL
                     NNPLKYTDPDGEWVHIVVGAVVGGVVNLAMNWNNCDGF"
     gene            4045..4329
                     /locus_tag="PKY68_00285"
     CDS             4045..4329
                     /locus_tag="PKY68_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23510.1"
                     /translation="MDMLLSNFVLPNLLCLNHIVPVSYHRFLSNPIGHNYYKIHLAIA
                     SKAKGKSGGARVITHLVFKDSTVYLLTIYDISEIENLTDKEILELIKLIP"
     gene            complement(4361..4831)
                     /locus_tag="PKY68_00290"
     CDS             complement(4361..4831)
                     /locus_tag="PKY68_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23511.1"
                     /translation="MKSSKIMIYTILIVIGFILLYQFGIKNKGLRDSIQSNGIETIGT
                     VVNRTKGVEDGGITTFGVHFDFYANGELIKSYQRLTGRIEYDNAIVGMKYKVKYLSEK
                     PNINSIIFINEPQKSEYKNIPKERERILNTYENAQVFLKKNAQPLDELNHLIEQ"
     gene            complement(4828..6135)
                     /locus_tag="PKY68_00295"
     CDS             complement(4828..6135)
                     /locus_tag="PKY68_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOH23512.1"
                     /translation="MGDYEEVYKPDGTIQKIHYLSGGSMMLQQGGTKSLYYGYHDYQG
                     SLIAWTNQSGTVVERRAYDPWGRHRNPQNWTEVDYTSPSQLNNRGYTGHEHLDAFQII
                     NMNGRVYDPLTAQFFSPDPFVQAPDNWLNYNRYAYCLNNPLIYTDPDGEWIHIVVGAV
                     VGGIINVATNWDNLDDFGEGLASFGIGAATGALTAVNPALGAVVGGAFTSAGNNVIAQ
                     TGNGVGLNQVNWGQVGGQAVLGAVVGGVTYGVGSAINQTGITNKILDASGITNNVVRN
                     IVGSTVNGVMVGTTAGLARGVGTGLLTGEWNVWEHTWKGAAYGGAGGFLYGSLTELGY
                     QAQLKFGRNDALNSKNTDAIAKGGQRYVNRLQKIDGSNNGGSGNSATGQGMGEITVVY
                     NTQTGTTTVSITVSPNSYWQPPAPYQYNSPTIYQNILRINKQQ"
     gene            6288..7817
                     /gene="ribF"
                     /locus_tag="PKY68_00300"
     CDS             6288..7817
                     /gene="ribF"
                     /locus_tag="PKY68_00300"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOH23513.1"
                     /translation="MELKGIKNILFDFGGVLVKLNPQACLDAFGALGAPQVADYLTPY
                     GHTGPFGLFENGNIGLEEFRKEIRRIFDIKAGDEEIDRAWAAFLEDIPLENTTIVHNL
                     SKKYRLFLLSNTNPIHIRKLEQFEQAGRPLNQCFEKCYLSYEIGFSKPRKEFFEHVLK
                     DSCLKAEECLLIDDGPANCRSAEALGMKTLCPGSVEDLSKFFPEMLNTVTAEKTVATI
                     GFFDGVHLGHKQLIALLRREAAQRNLASLVLAFWPHPRMVLHSDYCPQLLNDCSEKLA
                     LLRACGADHTRLVRFDKQLARMSAFEFMNQVLKKEWNVACLLAGYDHRFGKNKEEDFD
                     DYRRYGDVLGIEVLKAGPALLPDSSRAISSSYIREALLKGEILKANAALGYDYTIKGR
                     VGGGYGVGRKLGFPTANIIPSDPHKLIPLPASYIVLAEVEKRLYPGMLNIGQRPTLQN
                     GCQRSIEVHLIDFSGDLYDKEITVHFKKHLRPEKRFPSTAELSNQLKLDREAVLRFFK
                     Q"
     gene            complement(7932..8642)
                     /locus_tag="PKY68_00305"
     CDS             complement(7932..8642)
                     /locus_tag="PKY68_00305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOH23514.1"
                     /translation="MKKNVMLAVVFAGTMCLSLCFTSCDCKCGSKAMKTNVDSLSYAY
                     GVGLGDNLAQNLSSFPAELNMDLFLEAFEKTIKGKTEGLSIQPDQAYQVFQSCLTAIQ
                     MESAQKNKEEAGKFLEENAKREGVVTTESGLQYEVLLAAEGPKPTATDRVSVHYHGTL
                     LDGSVFDSSIDRGEPAQFALNGVIPGWTEGIQLMSVGSKYKFYLPSDLAYGDNGAGDV
                     IQPGAMLIFEVELLEILK"
     gene            complement(8748..10568)
                     /locus_tag="PKY68_00310"
     CDS             complement(8748..10568)
                     /locus_tag="PKY68_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOH23515.1"
                     /translation="MDYSFYDFLMLVGSLGLFLFGMKMMSEGLQKAASKKLKEFLSAM
                     TSHRYAGVLMGMLVTAIIQSSSATTVMVVSFVNAGLISLVQSISVIMGANIGTTVTAW
                     IISVFGFNFNIAELSIPLIGIGIIPLLFSKKNNHKNIGEFIIGFALLFMGLSYLKDSV
                     PDLQNNPEALEFISKFTQKGYGSVLIFLLFGTLLTIVIQSSSATMAVTLIMCAKGWIS
                     FEMGAAMVLGENIGTTVTANLAAISTNVSGKRAALAHTTFNVIGVIWMLIVFFPFVGM
                     IQKIIVNVTGVDPKQLDVFTAGLDPGVLNLISDPGITLDAPQLIRLRSQLVSLQASTS
                     YALSLFHTMFNLINTGLLIWFTGTIAKIVTKVIPSKKTDEEFQLRYISTNLLSASELA
                     IPEAKKEIDDYITRTRRMFDMVRDLYYEKQESNFEKIYARILKYESISDRVELEIATF
                     LNNVSEGRLSDKSKHQIRQMIRMTSEIESIADSCHNIANTIKRRWDAKVVLTDEMTEH
                     VEIMSGLVYKQLEGMANLILKNSHDTAELLKIRDAEIEINKYRNQLYQENDLDLEAKK
                     YTYQEALYYIDIIRECERLGDYIVNVVEAYTGGSKLFAHI"
     gene            10684..12348
                     /locus_tag="PKY68_00315"
     CDS             10684..12348
                     /locus_tag="PKY68_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOH23516.1"
                     /translation="MSKAVRIFCVNNNKNMLMPMGVSLLEVYQQLSQELNMEPIAARV
                     NNKTESLSYELYNPKRIEFIEMNSASGMRVYVRTLTFLISSAIYELYPEGSFSIEHSV
                     SNGYYCELDLKRECTRKDLDAIKKRVLELIEAGNPIDSVKEETSIVIELFRKRGRKDI
                     VDLLSTFGSLYTTYCKMGEHIDYYYGALAYSSDINKNFDLIPYLDNYLLRIPSRSNPS
                     ELAPLVPQPKMQDVFREFVRWNKIMDLRYVGDLNLMCRQGRATELIKVAEALQEKKIA
                     QLADTIAKGLDKIRIVLISGPSSSGKTTFSKRLSVQLLAMGIRPIAISMDDYFLEREQ
                     TPKDEKGEFDYESLYALDLDLFNRDLGNILEGQEVEIPSFNFALGTKEYKGNKVRLTP
                     QNILILEGIHALNPELLKNVPPEAQFKLYISALTAISLDKHNRIPTTDNRLIRRIIRD
                     SRYRGTSARETIARWDSVRAGEEKWIFPFQENADAMFNSALIFEFSVLKNYIEPVLRE
                     VPQNCVEYSEARRLLRFLDYFVPITDWEIPPTSLLREFVGGSSFRY"
     gene            12415..13848
                     /gene="rpoN"
                     /locus_tag="PKY68_00320"
     CDS             12415..13848
                     /gene="rpoN"
                     /locus_tag="PKY68_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOH23517.1"
                     /translation="MLHQELSLKLQQKLSPQQILRIKLLEIPELEMEERILRELDENP
                     ALELGGDPESKEESLEEEVEEQQDELDLEDYLQDDDDLYGNREYDKNTPDRRETFFGS
                     SALSLRDFLMQQLDMQNLTENEKIIGEYLIGNIDDDGYLRIPLETVSDQISFQAFKEV
                     SLDEMHKMLKVIQDFEPAGVAAQNLKDCLLLQLYRKPKTVLTEQAIHLLEQYFEEFSH
                     KHHDRILHRWGIAREDYDAIVGEILKLNPKPGNAWEGMPGESGKQIIPDFIIENIDGN
                     LVLSLNEGNLPEIHVSSRYLRLLQDYNASPHKQSEETRNAAIFARQKVEAAYWFIEAI
                     KQRQQTLLSTMQAIMDYQKEFFLSGNEKKIRPMILKDIANITSYDISTISRVSSSKYV
                     QTSFGIFPLKYFFSESLLTDSGEEVSTIRVKNVLQEIIDNEDKSKPYTDDELEKIMQD
                     KGYRVARRTISKYRSQLKIPVARLRVQ"
     gene            13882..14259
                     /gene="gcvH"
                     /locus_tag="PKY68_00325"
     CDS             13882..14259
                     /gene="gcvH"
                     /locus_tag="PKY68_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOH23518.1"
                     /translation="MNDPKTLKFTKNHEWIRVEGQEAYVGISDFAQSELGEIVYVDIT
                     TVGESLQKEEVFGSVEAVKAVSDLFIPVGGTVLEANAELEDHPELINKEPYDAGWIIK
                     ISLNNSAELQELLSYEQYKEMIG"
     gene            14264..14791
                     /gene="purE"
                     /locus_tag="PKY68_00330"
     CDS             14264..14791
                     /gene="purE"
                     /locus_tag="PKY68_00330"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOH23519.1"
                     /translation="MKAIVSIIMGSTSDLPVMEKAADLLNEFQIPFEMHALSAHRTPE
                     AVEKFSRTAAERGIKVIIAAAGMAAHLPGVIAASTTLPVIGVPIKASLEGMDALLAIV
                     QMPPGIPVATVGINGATNAALLAVEILAVADQELQARLIAYKTGLKNKIVKADRELQQ
                     LKYEYKTNDLLSESL"
     gene            14766..16622
                     /gene="ispG"
                     /locus_tag="PKY68_00335"
     CDS             14766..16622
                     /gene="ispG"
                     /locus_tag="PKY68_00335"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOH23520.1"
                     /translation="MTYCRNLFNYQRRKSSPVNIAQVPLGGSYPVRIQSMTNCSTLDT
                     VCCVEQIKKIVAAGADYVRLTAQGTKEAENLGNIRRVIRKQGVEVPLIADIHFNPKAA
                     EMAASQVEKVRINPGNFADPLHIELPEGGVSDLGGVSIPELEKQKIRERLLPLLAICK
                     KRGTALRIGVNHGSLSSRMMLRYGDSPRGMVESCMEYLRICVEEDFSQVVISIKSSNT
                     LAMIYTVRLLVKTMEAEGMNFPLHLGVTEAGDGEDGRIKSAVGIGSLLLDGIGDTVRV
                     SLSEDPEVEIPVARKLVEYVEERKGHALINAKAFEGFDPYSYKRRESKKAGIIGKEQV
                     PVVIQDHSQGLTTDYSFADPMPDFIYLGAKTPEAPRKDNIPQICDYRVYREQTQCYPL
                     FEVAQIEELSGCKAALRFLRVCHTDLNEGLLQQLEKEQNIVLWLYSSHLNAAAEMRAA
                     FHTLLNLGSYLPVVVAAAYSTEDVENLQVQAATDLGFLLADGFGDGVCISAGGLQAEC
                     LSLMFGILQATRSRISKTEYISCPGCGRTLFNLQSTLAKVKAGTSHLKGLKIAVMGCI
                     VNGPGEMADADYGYVGAGRGRISLYKGKLCVEKNIPEEEALERLLNLIQKDR"
     gene            complement(16738..18429)
                     /locus_tag="PKY68_00340"
     CDS             complement(16738..18429)
                     /locus_tag="PKY68_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23521.1"
                     /translation="MNKTFKLSILVSLLLILSGCGSKLALDREHFTVTPQVLEAVGGK
                     VDATIDGVFPAKMFPAKGIVTITPVLKYEGGEVEGAPMSFQGEKAVGNNKVIPTAAGG
                     NFSMKSSFDYKPEMKRSELFVRINSVTGNKTQDHPEIKLADGVVSTENLLNAQKAKPS
                     PGADKFQRIISEVTEAQILFLIQQSNVRTSELRSDEIKALVSAISNATQTENKEISGI
                     NVASYASPDGPVKLNESLAGNREKNTVNYLNRELRKAKAQGDINAEFTAEDWDGFKKL
                     LEASDVQDKALILRVLSMYSDPEQREAEIKKLSSAYKVLADEILPKLRRSKIALTVDV
                     IGKSDEEIKALFGSNPSELNVEEILYAATLYSTPAEKEKVYQKTTQLYPNDSRAFNNI
                     GMIKFEAGDVAGAKTWFEKAYTIDKSKESSLNLALCAISEGADNATVEAYLNQAAGAE
                     NYEGALGLLYIKKGDYANAVKAFGDQKSNNAALAQILAKDYSKAKATLAGVEKPDAET
                     YYLQAIVGARTNNKAEIVDGLKKSIAQDPAMAKKAANDIEFAKYLVDAEVASLLS"
     gene            complement(18584..>19395)
                     /locus_tag="PKY68_00345"
     CDS             complement(18584..>19395)
                     /locus_tag="PKY68_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH23522.1"
                     /translation="RHENPENGFLFGHNSFEYKVFAEEFRQDSNSMLKGVYLMPSKGV
                     KSSSYPVYIDVMKGGFVPEETLASVELNPYYLEYRNRNFHETSKVHFANAENYVCFDP
                     PLSVGKHFYVAIRLVYPSKTVAAIDSFALYSAYHKERTAKNTVWVNNGTDWEFFTAMN
                     RRAHSSSLWLEPVVMKDTLPVVEPPLVVDTLSQRIPLYYSLQDRLLHISFPTGWRSPF
                     EIRIFDPAGRLMHFTSTEQETMTLYIPESFKGFYIIQLTERRRRYVQKIVF"
BASE COUNT         5335 a         4469 c         4280 g         5311 t
ORIGIN      
        1 cgtcacctta ccttgctgct ccagcctgct gaaatcggga gtgagttcaa gaacctcgtc
       61 ggtaccgttg atcccggcta tgtaccagcg gtctccgctg cgacgggcta tgaccgcact
      121 ctcgccggga tatcccgcaa gcaagcgggt gtcgtcccat accgaaggca gccccataag
      181 caggtctttg acttggtaag gcagcgacag ataggattcc ggacggtcag ccatatgttg
      241 cagacccgac tcaaagagta tgggaagcgc caactcgtga caatcggtag tgatatgctt
      301 gtgctgactg tccgaaaagg tgcagggagt atagtccata gggcctatca cgttgcgcgt
      361 aaaaggcagc gtggcattgt gtgaggctgc ggctttggtc atacggggcg tgttgttgta
      421 ccactcggcg ccataaacgc cctccatggt cataagattg ggccaggtgc ggttccatcc
      481 gcgcggaaca gtgcagccgt ggaaatttac cagcagccgg tggcgggcag catcctcaag
      541 tatatcaagg taataatcta tcatatccgc accatcgggc aagaaaaagt ccaccttgat
      601 gccgcttact cccatattgt tcaaacgtgt catctcactc tcgcgggcct cggccgtacg
      661 cagacgatcc aggggagtcg gagcgcccgg acctacccag gaggtgccgg agttgtacca
      721 taagttcacc tttataccct tttcccgggc gtatgccatg gcatcctcaa tgtcgcctcc
      781 tcccgccatc tcgtcccact cccaatctat cagggtgtag ggccaactca tctcggcggc
      841 caggtcaatg tattccttta ccttaccgaa atctttcgac gagcggttgt atgcccaata
      901 tatccacgat gagagaccag ggtggatcca ggagatatcc ttaatttggc acggggtggc
      961 cacatcggtc accagagttg attccacaat atcggcaagc gagcctataa taagcacaaa
     1021 agccggagaa ataccgtttt cggacttacc ttcggacata tatttaatat ggtaagcgtt
     1081 atcatcccga acttccagat gggagccgct cataccgtgc tgtacgtccg attcagaaat
     1141 cagtccgaac agaccatcgg catactcggt aagcagcgga tagccgtaac tcccgctgcg
     1201 tgcctccgtc cgcatgggat agaagccctc ataatcggtc ctcaggttct gtgaccatcg
     1261 tttctggcca tcgggaatta tatatgatat ttccggatct gtcccgtcag ttatttttct
     1321 cagaacaacg ccgtcattaa aaactctcag gtcaagaaca agaccttcac atcttagcaa
     1381 catctgtctg aaaacattag agcagtcact ccttttgccg ctaagcatat ggtattcgag
     1441 gggcgtgtca ccggttttca atgcaacagc gtttttgccg acaggagtta tatccatttt
     1501 tatgaattgg atcggcctct tttcataact gaccatcagt cccgaatcgg ttacactgac
     1561 cccgatttcg ccactcggag attttaaaac atatctcttt cctgcaccgg cacatgccga
     1621 caggagaata gaggaaatga gaataagata aatttttgac ttcatggtta aggttcatta
     1681 ttggttgcaa agttaataag ctcacgatac aagcgctgcg ccggcagacc caaattagtt
     1741 accagccaaa gttagcaact tttgggaggt attcgtattt tcccttattt gtcttcgcaa
     1801 accggtgtac tcaggtgata ccaccaccca cgattggaat aaagcacgag gacaaggcgg
     1861 ccttcctgat cgtccacacg ggttaaatga acgttttcat cgtaggtatt gagtacgtaa
     1921 taacacaaag gatttctata gatttctatg acatattatg tggatttatt gtttttgagg
     1981 agtactatga ttgtatgatt agatgaataa gctgataatt taagtttctg tctccatctc
     2041 catgaataaa aaagaccctt aggtttgtcg aaaaaaacat aaccaaagtg aacttcattt
     2101 tctctcaaat agaaaaaagc aaaattgcaa agtataatgt aacaataaag ccggatttct
     2161 ccggctttat tgttgtgact cactatttcg ttgcattatc cagttataaa aatattcgca
     2221 agcttctgtt tcgctactaa actcttttat atcaaactta gagcctcttt cggtataaaa
     2281 aacttcccat ttattgtttt tttcttccaa gcaataactt tctccaggaa gcataaattc
     2341 aaaatagtta gcatacctaa attttaataa atcccacgga tgtttaagac ttattggata
     2401 tttctctttt ttaagctgtg ttttaatctt gctttttaat tgctttatat tcattaagat
     2461 actcttatta tataactatt ctgcaacaaa taattgatat tataatcaaa catgtactgt
     2521 gtaccacctc ctgatgaatt gaaccaaaat gagcctgagg atgtgccttg atacgctgga
     2581 agaggattaa ctactttgta ggtagtaaga ggcgataatt tagcattagg aggtagtgaa
     2641 cgtgagtcaa atggggtacc atcaggagct gcaaatcgac ctgtttcatc cccgtatcta
     2701 tcaaaggttg tacctccttt tagtattgtg cgttctggac taccgttaaa tccattattt
     2761 ttaggccaca ttgttttgcc agttatagga tttactccct ttgcaaagct cattccaact
     2821 gttgttgtta ctcccagtgc agcacccatt agcatactgt ttccaatacc tttaaacgaa
     2881 ttctcaaaag cagtattaaa actttggcct tgtaataaac cagcagtagt tcctcctgcg
     2941 atgtgtccag cacctgcggc aagtggagaa acaatggctg aacgtaaaat tggactatta
     3001 ataccactta caagcattga cgaatttgca gcccaatatc cagcagcact acctgcaaaa
     3061 ccagaaacac tgccaatagc ggaactaatt ccaacctgtc cccagtcaac atttcccatt
     3121 cctgaaaaat tatgacctgt ttgggctatt atactattag ttcccattgt tgccgcactt
     3181 ccggctgctg caactcctgc agttgcccaa aaaccagctc ctgctgctcc tcctgttgca
     3241 gcggttgctg cccctgctcc agctccaacc acaaacgctg ctgcatattg tcaaaaacca
     3301 tcacaattgt tccaattcat ggctaagttt acaacaccac caactacagc accaactaca
     3361 atatgaaccc attccccatc ggggtcagta tatttgagcg gattattcag gcaatacgca
     3421 taccggttgt aattcaacca attgtcgggg gcttgtacaa aggggtcggg gctgaagaat
     3481 tgggcggtta atgggtcgta tacccggccg ttcatgttga tgatttggaa ggcgtccagg
     3541 tgttcgtggc cggtgtagcc gcggttgttg agctgggagg gggatgtgta gtcgacctct
     3601 gtccagtttt gggggttgcg gtggcgaccc caggggtcgt aggctcggcg ttccactacc
     3661 gttccgctct ggttggtcca tgctatcaaa gaaccctggt aatcgtgata tccgtaatac
     3721 aaggacttgg tgcctccttg ttgcaacatc atcgaaccgc cgctaagata atggattttt
     3781 tgaattgttc catccggttt atacacttct tcgtaatcgc ccaaataata acgggtagtt
     3841 gtggcagagc cctgagtaaa gacagacttt ctgcgctggt cgtccacacc gtagcttatt
     3901 tcgtaaagcc ggttgctctc tgtcagggtc tttattttac tgaagtcggt gtaggttagc
     3961 gtcagatttc cggaagggat ataatcagaa aacgttgctg cattatgacg aaaaagtcgg
     4021 ggtagcccgg cttctcagtt tcctatggat atgttattgt ccaattttgt tcttccaaat
     4081 cttttatgct taaatcatat cgttccagta tcctatcacc gcttcttatc aaatcccatt
     4141 ggacataact actataaaat acaccttgcc attgccagta aggccaaagg gaaatcaggc
     4201 ggggcaaggg ttattaccca tcttgttttt aaggatagta ccgtttacct tctcaccatt
     4261 tacgatatat cagaaattga aaaccttacc gataaagaaa ttctggagct tataaagtta
     4321 attccataaa aaagccccga ttcccccgag gcttttgggt ttattgttct atcaaatgat
     4381 ttagctcatc taatggctgg gcattcttct ttaaaaacac ttgggcattt tcatatgtat
     4441 tcaagatacg ctccctttct ttaggtatat ttttgtattc cgatttctgg ggctcattta
     4501 taaaaattat agagttgata ttaggctttt cagataaata ttttacctta tatttcattc
     4561 caacaatagc gttatcatat tctattctac cagttaacct ctgataagat tttattaatt
     4621 ccccatttgc gtaaaaatca aaatgaactc caaatgtagt tatacctccg tcttcgacac
     4681 cttttgtccg gtttacaacc gtccctattg tttcaatacc attactttgt atagagtctc
     4741 ttaatccttt atttttaata ccgaactgat ataaaaggat aaaaccgatt actattagta
     4801 tggtataaat cattatttta ctacttttca ttgttgctta tttattcgaa gtatgttttg
     4861 ataaatagta gggctattgt attgataagg tgcagggggt tgccaatagc tatttggact
     4921 aacagttata gatacagttg ttgttcccgt ttgggtgtta taaacaacag taatttctcc
     4981 cataccttga cccgttgcag aatttccact tcccccgttg ttactcccat ctattttttg
     5041 taaacgattc acatatcgct gaccaccttt agcaatagca tctgtatttt tactatttaa
     5101 agcatcattg cgcccaaatt ttaattgagc ttgataacca agttctgtta aactgccata
     5161 taaaaagccc ccagcacctc cataagcagc tcctttccac gtatgttccc atacattcca
     5221 ttctccagtt aaaagaccag taccaacacc ccttgctaat ccagccgttg tgccaaccat
     5281 aactccattg acagtagaac caacaatgtt tcttacaaca ttattcgtta ttccagatgc
     5341 atctaatatt ttgtttgtta tacctgtttg attaatcgca gaaccaaccc catatgttac
     5401 acctccgaca acagcaccta aaacggcctg accgccaact tgtccccaat taacctgatt
     5461 taatccaaca ccattgccag tctgggcaat tacattattt cccgcacttg taaacgcacc
     5521 tccaaccact gctcctaatg ctggatttac cgcagtcaag gctcctgttg cagcaccaat
     5581 tccaaatgaa gctaaacctt ctccaaaatc atcaagatta tcccaatttg tggctacatt
     5641 aataatccct ccaactacag ctccaacaac tatatgtatc cactccccat ccggatccgt
     5701 ataaatcagc ggattattca aacaatacgc ataccggttg taattcagcc aattgtcggg
     5761 agcttgtaca aaggggtcgg ggctgaagaa ttgggcggtt aatgggtcgt atacccggcc
     5821 gttcatgttg atgatttgga aggcgtccag gtgttcgtgg ccggtgtagc cgcggttgtt
     5881 gagctgggag ggggatgtgt agtcgacctc tgtccagttt tgggggttgc ggtggcgacc
     5941 ccaggggtcg taggctcggc gttccactac cgttccgctc tggttggtcc atgctatcaa
     6001 agaaccctgg taatcgtgat atccgtaata caaggacttg gtgcctcctt gttgcaacat
     6061 catcgaaccg ccgctaagat aatggatttt ttgaattgtt ccatccggtt tatacacttc
     6121 ttcgtaatcg cccaaataat aacgggtagt tgtggcagag ccctgagtaa agacagactt
     6181 tctgcgctgg tcgtccacac cgtagcttat ttcgtaaagc cggttactcc ccccaacacc
     6241 aaaattaatt ttgtcgttgg tctcgcttgc gctatctttg cccggcgatg gaactaaagg
     6301 gaataaagaa tattttgttt gattttgggg gggtgctggt caagctcaat ccgcaggcct
     6361 gcttggatgc ttttggggcg ctgggagcgc cacaagtggc agattatctc acgccgtacg
     6421 ggcataccgg accgtttggt cttttcgaaa acggcaatat cggcctggaa gaatttcgga
     6481 aagaaatcag aagaatcttt gacatcaagg ccggcgacga agaaatagac cgggcctggg
     6541 ctgcttttct cgaagatatt ccactcgaaa acacgactat agtccataac ctgagcaaaa
     6601 agtacaggct ctttttgttg agtaatacca atcccataca tatccgtaaa cttgagcagt
     6661 ttgagcaggc ggggcggcct ctaaaccaat gctttgagaa atgctacttg tcctacgaaa
     6721 ttggcttttc caagccccgg aaagagtttt ttgaacacgt actcaaagac agttgtctca
     6781 aggcggaaga atgtctcctt atcgacgacg ggccggccaa ttgtcgcagt gcggaagccc
     6841 tgggaatgaa aactctttgt cccggcagcg tggaggattt gagcaagttt tttcccgaaa
     6901 tgctaaacac ggttacagcg gaaaaaacag tggccaccat cggttttttc gacggtgttc
     6961 acctgggaca taaacaactg atagccttgc tccgtcgaga ggcggcgcaa agaaacctgg
     7021 cctcgctggt gctggccttt tggcctcacc ccagaatggt cttacacagc gattattgtc
     7081 cgcaactatt gaacgattgt tccgaaaaat tggctctgct gcgggcctgt ggcgccgatc
     7141 atacccgact tgtacgtttc gacaaacaat tggcccgaat gtcggctttc gaatttatga
     7201 accaggtcct gaaaaaagaa tggaacgtgg cttgtttgct ggccggttac gatcatcgct
     7261 ttggtaaaaa caaggaagaa gacttcgacg attatcgccg ttacggcgat gtgttgggta
     7321 ttgaagtttt aaaagccggc ccggccttac ttcccgattc ttccagggcc atcagttcct
     7381 cttacatcag agaggccctg ctaaaaggag aaatattgaa agcaaacgcc gccctgggtt
     7441 acgattacac catcaaaggc agagtaggcg gcggttacgg agtgggtcgc aaacttggat
     7501 ttcccacggc caatattatt ccttccgatc ctcataaact tatacccttg cccgccagtt
     7561 acatcgtttt ggcagaagtc gaaaaaaggc tgtatcccgg catgttgaat ataggacaac
     7621 gccccacctt gcagaatggc tgtcagagga gcattgaggt gcatttaatc gacttttcgg
     7681 gcgacctcta cgataaggaa atcaccgttc atttcaaaaa acaccttaga cccgaaaagc
     7741 gtttcccatc cactgcagag ctgtcgaatc aactcaaact cgacagagag gccgtattac
     7801 gcttttttaa acagtaaaag agtcgtctca aagcgaactt taagacgact ctttaaaatg
     7861 tatttttgtc tccggtaagc ttgccgggcc aataaaaacg gctaacgaat taccgtaaac
     7921 aaatcattct tctatttaag gatttcgagc aattctactt cgaaaatcaa catggcgccc
     7981 ggttggataa catcgccggc accgttgtca ccgtaagcca aatcggaagg caaataaaat
     8041 ttgtatttgg aacctacgct cattaattga atgccttcgg tccatccggg tataacaccg
     8101 ttcaatgcaa attgggcagg ttcgccgcga tcgatggaac tgtcaaagac agaaccgtca
     8161 agcagagtac cgtggtaatg cacactaact cggtctgttg ctgtaggctt aggaccttcg
     8221 gccgccaaga ggacttcgta ttgcaatccg ctttcggtag ttacaacgcc ttctctctta
     8281 gcgttttctt ccaggaattt accggcctct tctttgttct tttgagcgga ttccatttgt
     8341 atggccgtca aacaactttg gaatacctga taagcctgat cgggctggat gctgagacct
     8401 tcggtctttc ccttgatggt tttctcgaaa gcttccaaaa acagatccat attcagttcg
     8461 gcgggaaaag agctgaggtt ttgcgccaaa ttgtctccca ggcctacgcc ataggcatag
     8521 ctaaggctgt ctacgttggt tttcattgct ttacttccac acttgcagtc gcaagaggtg
     8581 aaacataagc ttaaacacat ggtgccggca aaaacgacgg ctaacattac atttttcttc
     8641 ataattctaa tttttgatgt atttgttgtt gaaattctat ccgtgataat caaaagtttg
     8701 caaagataag acaaaaactt cggatcagac gaaattgatg accttattta gatatgagca
     8761 aacaatttag atcctcccgt ataagcttcc accacattga caatgtagtc gcccaacctt
     8821 tcgcattcgc gtataatatc tatatagtac aacgcttcct gataggtgta ttttttggcc
     8881 tccagatcga ggtcattttc ctggtacaac tggttgcggt atttgttgat ctctatctcc
     8941 gcatctctga ttttgagtaa ttcggcggtg tcgtgactat tttttaaaat cagatttgcc
     9001 atgccttcca actgtttata caccaaaccg gacataattt cgacatgttc ggtcatttca
     9061 tcggtcaaaa ccacctttgc gtcccagcgc ctcttgatgg tgttggcaat gttgtggcaa
     9121 ctgtcggcta tactttctat ctcggaggtc atgcgaatca tttgcctgat ttgatgctta
     9181 cttttatcgc tcagacggcc ttctgaaaca ttattcagga aggtggctat ttcgagttct
     9241 accctatcgc tgatactttc atatttcaat atgcgggcat agatcttctc aaagttggac
     9301 tcttgttttt catagtataa atcacgcacc atatcaaaca ttctccgggt tctggtaatg
     9361 taatcgtcga tttctttttt ggcttcgggt atggccaatt ccgaagcaga cagcagattg
     9421 gtgctgatgt atctcaattg gaattcttcg tcggtctttt tactggggat aaccttggtg
     9481 actattttgg ctatcgttcc cgtaaaccaa atcaacaaac cggtgttgat aagattaaac
     9541 atggtatgga acaacgaaag cgcgtaagat gtgctggcct gcaaggatac taattggctg
     9601 cgtaagcgta ttaattgagg agcgtcgagg gttattccgg ggtcggaaat caagttaagc
     9661 accccaggat ccaggccggc agtaaagaca tccagttgct tggggtcaac accggttaca
     9721 ttaacgatga ttttttggat catgcccaca aaagggaaaa acacaatcaa catccaaatc
     9781 acaccgatca cgttgaaggt ggtatgcgcc aaggctgccc gcttacccga cacattagtg
     9841 gaaatcgctg ccaggttggc cgttaccgta gtccctatgt tttcgcccag gaccatggcc
     9901 gctcccattt caaaactgat ccatcccttg gcacacataa tcagggtaac cgccatagta
     9961 gcgctggaag actgaatcac aatggtcagc aaggtaccaa agagcaaaaa aatcaaaacg
    10021 gagccgtagc ccttttgggt aaatttggat ataaattcaa gcgcttccgg attattttga
    10081 agatcgggca cggaatcttt caagtaagaa agccccataa acaaaagagc aaaacctata
    10141 atgaattctc caatgttttt gtgattgttt ttcttggaaa acagcaaagg tataataccg
    10201 attccaatca ggggaatcga gagttccgct atgttgaaat taaatccaaa gaccgaaata
    10261 atccaggctg tcacggtagt accaatattg gctcccataa taacgcttat ggactgcacc
    10321 agacttatca accccgcatt gacgaaacta accaccatca ccgttgtggc cgaagaagac
    10381 tggataatgg ccgttaccaa cattcccatc aagactcccg cgtaacggtg cgaagtcata
    10441 gccgaaagga attcttttaa tttcttactt gctgcttttt gtagcccctc gctcatcatt
    10501 ttcatcccga aaaggaacag gcccagggaa ccgactagca tgaggaaatc ataaaaagaa
    10561 taatccatag aagctgtgta aatctgaatt ttgcttttgc gccacaaaag taagtgaaac
    10621 ttttttaatt acagaatcta tggaaattta attgtacctt agagccgatt tttcactatt
    10681 gctatgtcaa aagccgttcg tattttttgt gtaaataaca acaaaaacat gctcatgccc
    10741 atgggggtaa gcttgctgga agtttaccaa caactttcgc aagagcttaa catggagcct
    10801 attgcagcca gagttaataa caagaccgaa agtctgagct atgaattgta caatcccaaa
    10861 aggatcgaat tcatcgaaat gaattctgct tcgggtatgc gggtatatgt acgcaccctg
    10921 acttttttga tctcctcggc catttacgag ctttatcccg agggcagttt cagtattgaa
    10981 cattccgttt cgaacggcta ttattgtgag ctggatctga agcgggaatg cacccggaaa
    11041 gatcttgacg ccatcaagaa aagggtactt gaacttattg aagccggcaa tcctatagat
    11101 agcgtaaaag aagaaacctc catagttatt gaattgttcc gaaaaagagg cagaaaagac
    11161 attgttgatt tgctgagtac ttttggcagc ctctacacta cttactgcaa aatgggggaa
    11221 catatcgatt attattacgg agctttggcc tattcatcgg atattaataa gaattttgac
    11281 ctgattccct acctggacaa ttatttattg cgcatcccat caaggtcaaa tccctccgaa
    11341 ctggcaccct tggtgcccca acccaaaatg caggatgttt tcagagaatt tgtccgttgg
    11401 aacaagatta tggatcttcg ctatgtgggc gatttgaatc ttatgtgccg tcagggcaga
    11461 gcaacggagt tgatcaaggt ggccgaagcc ctgcaggaaa aaaagatcgc tcagttggcc
    11521 gacaccattg ccaaggggct ggataaaatt cggatagtgc tgatttcagg gccttcttcg
    11581 tcgggcaaaa ccacctttag caaacgttta tccgtacaat tgcttgctat gggaatacgc
    11641 cctatagcca tttctatgga tgattatttt ttggaaagag agcaaacacc taaggatgaa
    11701 aagggcgaat ttgattacga atcgctttat gccttggatt tggatctttt caatcgcgat
    11761 ctgggcaaca tactcgaagg gcaggaagtg gaaattccca gctttaattt tgccctggga
    11821 accaaagaat acaaaggcaa taaagtcaga ctgacgccgc aaaacatcct tattctggaa
    11881 ggcattcacg cactaaatcc cgaattgttg aaaaatgttc cgcctgaagc ccaatttaaa
    11941 ttgtatattt cagcccttac cgccatttcg ctggataaac acaaccggat tccaaccaca
    12001 gacaacaggc tgatccggag aattatccgg gattcccgct accggggaac ttcggccaga
    12061 gaaactatag cccgctggga tagtgtccgt gccggagaag aaaaatggat ttttcccttc
    12121 caggagaatg ccgatgccat gtttaattcg gcactgatct ttgaattctc tgtattaaaa
    12181 aattacatag aacccgtatt gagagaagtc ccccagaatt gcgtcgaata ctccgaagcg
    12241 agacgtttac tgcgcttcct ggattacttt gtccccatta ccgattggga aattcctccc
    12301 acttcgctac taagagaatt tgtcggaggc agtagttttc gttattaacc gagtcaccgc
    12361 tgattcaatc gaaaaatgct acttttgcac aaaagtttta attgacggaa caacatgtta
    12421 caccaggaac tttcgttaaa gcttcaacaa aaactttctc ctcagcaaat tctgagaatc
    12481 aagctgctgg agattccaga attggaaatg gaggagcgta ttctgcggga gttagacgaa
    12541 aatcctgccc tggaactggg aggagatcct gaaagcaaag aagagagcct cgaggaggaa
    12601 gtagaagagc agcaagacga attagacctg gaagattatt tgcaagacga cgacgatctg
    12661 tacggaaacc gggaatacga caaaaacacc cccgaccgca gggagacttt ttttggatca
    12721 agtgccttgt ctttgcgaga ttttctgatg cagcaacttg atatgcagaa tctcaccgaa
    12781 aatgaaaaaa taatcggtga atacctcata ggaaacatcg acgacgacgg ctatctgcgc
    12841 attcctctcg aaacggtttc cgatcagatt tcttttcagg cattcaagga agtatccttg
    12901 gacgagatgc acaaaatgct caaggtgatt caggatttcg aacccgccgg tgttgccgcc
    12961 cagaatctta aagattgtct gttgctgcaa ctttaccgaa aacccaagac tgttttgacg
    13021 gagcaggcta tccacctgtt ggaacagtat tttgaagaat tttcgcacaa gcaccacgac
    13081 cgcatcctcc atagatgggg catcgctcgt gaagactacg acgcaatagt tggagaaatt
    13141 cttaaactca atcccaaacc cggcaacgct tgggaaggaa tgccgggcga atcgggcaaa
    13201 cagattattc cggattttat tatcgagaat attgacggta atcttgttct cagtctcaac
    13261 gaaggcaacc tacccgagat tcacgtaagc agccgctatc ttagattact ccaggactac
    13321 aacgccagtc cgcataaaca aagcgaggaa accagaaatg cagccatttt tgcccgtcaa
    13381 aaagtcgaag cggcctattg gtttatcgag gctataaaac aacgacagca aaccctgctc
    13441 agcactatgc aggccatcat ggactatcaa aaggagttct ttctgagcgg caatgaaaaa
    13501 aaaatcaggc cgatgatcct caaagatatt gccaacatca cctcttacga tatttccacc
    13561 atatcgaggg tttcgagcag taaatacgtg caaacatctt ttggaatctt tcctttgaaa
    13621 tatttctttt cagaatcgct gctaaccgac tccggagaag aagtttccac catcagggta
    13681 aaaaatgttt tgcaggaaat catcgacaac gaggacaaga gtaagcctta caccgacgac
    13741 gagttggaga aaattatgca ggacaaagga tacagggtgg ccaggcgcac catttccaaa
    13801 taccgttcgc agttgaagat cccggtggcc aggttgcgtg tacagtaaaa cagcttttct
    13861 attttaaata cttataagaa tatgaacgat cctaaaacat taaaatttac caaaaatcac
    13921 gaatggattc gtgtagaagg gcaagaagcc tacgtaggta taagtgattt tgcacaatct
    13981 gaactgggag aaattgtcta cgtagacatc acgaccgtag gagagagctt gcaaaaagaa
    14041 gaagtttttg gcagtgttga ggccgtcaag gccgtttcgg acctttttat tcccgtagga
    14101 ggaaccgtgc tcgaagccaa cgctgaattg gaagatcatc cggaactcat caacaaagaa
    14161 ccttacgacg caggctggat aataaaaatc agcttgaata attccgcaga gctgcaggaa
    14221 ctgctcagtt atgaacaata taaagaaatg ataggataaa cctatgaaag cgattgtcag
    14281 cataattatg ggcagtacct ccgacttgcc tgtcatggaa aaagcagcag acttactgaa
    14341 cgaatttcaa attccctttg aaatgcacgc gctgtcggct catcgcaccc ccgaggcagt
    14401 tgaaaagttt tcgaggaccg cagccgaaag aggaatcaaa gtaattattg ccgctgcagg
    14461 catggctgca cacctgcccg gagttattgc agcttccact actctgcccg tcattggagt
    14521 gcctattaaa gccagtctcg aaggcatgga tgccttactg gccattgtac agatgcctcc
    14581 gggcatcccc gtcgccaccg taggcatcaa cggagcaacg aacgcagccc tgctggcggt
    14641 ggaaatactg gcggtggcag accaggaatt gcaagcccgg cttatcgctt ataaaaccgg
    14701 acttaaaaac aaaattgtaa aggccgaccg ggaattgcaa cagcttaagt acgaatacaa
    14761 aaccaatgac ttattgtcgg aatctcttta actaccaacg tagaaaaagc tccccggtaa
    14821 acatcgctca ggttcctctg ggcggatctt atcctgtacg catacagtcg atgaccaact
    14881 gttccacctt ggatacggtc tgttgtgtgg aacagataaa gaaaatagtc gccgcaggag
    14941 ccgattatgt gcgcctaacg gcacagggaa ccaaagaggc cgaaaatttg ggcaatattc
    15001 gcagggtaat acggaaacag ggagttgaag tccctttgat cgccgatatt catttcaatc
    15061 ccaaggctgc cgaaatggct gcttcccaag tagaaaaagt ccggatcaat ccgggtaatt
    15121 ttgccgatcc tctgcacatt gagctgcccg agggcggggt aagtgatctt gggggagtaa
    15181 gtattcctga gctcgaaaaa caaaaaatca gggaacgact gttgcctttg cttgctatat
    15241 gcaaaaaacg cggtaccgct ctgcgcatcg gcgtcaacca cggatcgctt tcgtcgagaa
    15301 tgatgcttcg ttacggagac agtccccggg gcatggtgga atcctgcatg gaatatctgc
    15361 gcatttgcgt ggaagaagat ttcagtcagg tagtcatctc cattaaatcg tccaacaccc
    15421 tggctatgat ctatactgtg cgcttgctgg taaagaccat ggaagctgaa ggaatgaatt
    15481 ttcctttaca cttgggtgta accgaagccg gagacggcga agacggacgc atcaaatcgg
    15541 ctgtgggtat tggtagcctg ctgttggacg gaattggaga taccgtgcgg gtatcgctta
    15601 gcgaagatcc tgaggtagaa attccggtag ctcgcaaact ggtggaatac gtggaagaaa
    15661 gaaaagggca cgctttaatc aatgccaagg ccttcgaagg ttttgatccc tacagctaca
    15721 agcggcgcga gagtaaaaaa gccggaatta tcggaaagga gcaggtccct gttgtcatac
    15781 aagatcattc acaagggctt acaacagatt attcttttgc cgaccctatg cccgatttca
    15841 tctatctagg agccaaaacc cccgaagctc cccgcaagga caacattcca caaatctgtg
    15901 attacagggt atatcgagag cagacccaat gctatcctct gtttgaagtc gcacaaatcg
    15961 aagaattatc gggctgtaag gcagcactgc gttttctgcg cgtctgccac acagacctga
    16021 acgaaggatt attgcagcaa cttgaaaaag agcaaaatat agtactttgg ctgtacagtt
    16081 cccatttgaa tgccgcagca gaaatgcggg cggcttttca taccctatta aatctgggct
    16141 cctatttgcc cgtcgttgtt gctgccgcat actccacaga agatgtcgag aatctgcaag
    16201 ttcaggctgc aaccgatttg ggttttttgt tagccgacgg ttttggcgac ggtgtgtgca
    16261 tctcggcagg aggcttgcag gcagagtgcc tctcgttaat gttcggtatt ttgcaggcca
    16321 cccgctccag aatcagcaaa accgaataca tctcctgtcc gggttgcgga cgaactctgt
    16381 tcaatcttca aagcaccctg gccaaagtaa aagccggcac ctctcatctg aaaggcctca
    16441 agattgccgt aatgggctgt atcgtaaacg gtccgggcga aatggccgat gccgactatg
    16501 gttatgtggg ggcaggcaga ggccggatta gcctctacaa aggcaagctt tgcgtcgaaa
    16561 agaacattcc cgaagaagaa gcgttggagc gcttgctgaa cttgattcag aaagaccgct
    16621 gatacagcag ttctgcggat gtgttttttt gcgcataaga aaaccccgaa agtcttttat
    16681 ttaactttcg gggttctgtc atttttaaaa gcaccctttg ggggaattgt atcgtgctta
    16741 gctcagaagg cttgcaactt cggcgtcgac cagatatttt gcaaactcaa tatcgttggc
    16801 cgctttttta gccatggccg gatcttgagc tatggacttc ttcaaaccat ccacgatttc
    16861 agccttatta ttggtgcgcg cacccacaat agcctgaaga taataggtct cagcatcggg
    16921 tttttcaact ccggccagag tagctttggc tttgctgtaa tctttggcca aaatctgagc
    16981 cagagcggcg ttattgctct tctgatctcc aaaggcttta acggcattgg cataatcgcc
    17041 tttcttaatg taaagcagac ccagggctcc ttcgtaattt tcggcaccgg cagcctgatt
    17101 cagataggct tcgacggtag cattgtcagc tccttcggaa atggcacaca gagccaggtt
    17161 gagagaagat tctttggatt tatcgatcgt ataggctttt tcgaaccagg ttttggctcc
    17221 ggcaacatct ccggcctcaa attttatcat ccctatgttg ttgaatgcac gggaatcgtt
    17281 gggatatagt tgcgttgttt tttggtaaac tttttccttt tcggcaggag tactgtacag
    17341 agtggcagcg taaagtattt cttctacgtt gagctcagag ggattggagc cgaacaacgc
    17401 cttgatttct tcgtctgatt taccgataac gtcaacagtc aaagctattt ttgaacgtct
    17461 gagtttgggc agaatttcat cggccaacac cttgtaggca gacgacaact ttttgatttc
    17521 tgcttcgcgt tgttcaggat cggaatacat agacaaaaca cgcagtataa gagctttgtc
    17581 ctgtacgtcg gaagcttcca gcagtttctt aaatccatcc cagtcttcgg cggtaaactc
    17641 agcattgata tcaccttggg ctttggcttt tctcaattct ctattcagat agttgaccgt
    17701 gtttttttca cgattgcctg ccaaagattc gttcagctta acggggccgt cgggagacgc
    17761 gtaagaagca acattaatgc cggaaatttc tttattttca gtctgagtag catttgaaat
    17821 agcagaaacc agggccttaa tttcatcgct tctgagttcg ctggtgcgaa cattggattg
    17881 ttgaataagg aaaaggattt gagcttcggt tacttcagaa atgatacgct gaaacttgtc
    17941 tgcgccgggt gaaggtttag ccttttgagc attcaacaga ttttctgtgg ataccacacc
    18001 atcggccagt ttaatttcgg ggtgatcttg tgttttattg cccgtaactg aattaatgcg
    18061 gacaaaaagc tctgagcgtt tcatctcggg cttgtagtcg aaggaggatt tcatgctgaa
    18121 gttaccaccg gcagccgtag gtataacttt gttgttgcca acggcctttt ctccctggaa
    18181 gctcatcggt gctccctcga cttcaccacc ttcgtatttc aaaacagggg taatagtaac
    18241 tataccctta gccggaaaca ttttggcagg gaaaacacca tcgatggttg catccacttt
    18301 cccacctacg gcttcaagaa cctggggtgt aactgtaaaa tgctcacgat ctaatgctaa
    18361 cttagagccg catcccgata aaatcaacaa aagggatact aggattgaaa gtttaaaggt
    18421 tttgttcatg gatcaatgta ttatgttgtt agtaaaaatt tcaacccaca aatatacatt
    18481 taaaatgaaa catttaattg aatccttcgg tatttgggca agaaaattaa caatttaaca
    18541 gattttagtc cttaaagccg tgtgttttgg ctatttacac gctttagaaa acaatttttt
    18601 gcacatatcg ccgcctgcgc tcggttaact gaatgatata aaatcctttg aaactttcag
    18661 gaatatacaa cgtcatagtt tcctgttcgg tcgaagtaaa gtgcatcaga cgaccggccg
    18721 ggtcgaaaat gcgaatttca aaggggctgc gccagcccgt cgggaacgag atatgcagca
    18781 atctgtcctg caaagaataa tacagcggaa ttcgttgact gagcgtatcg acaacaagag
    18841 gaggctctac gacagggagc gtgtctttca ttaccacagg ctcgagccac agtgaactcg
    18901 aatgagctct gcggttcatc gctgtgaaaa attcccagtc tgttccattg tttacccata
    18961 cggtattttt ggccgttctc tccttatggt aggctgaata caaagcaaag ctgtcaatgg
    19021 cagccaccgt tttggaagga tagactaagc gaatagccac ataaaaatgt ttacctaccg
    19081 acagaggggg atcaaaacag acataattct cggcgttggc aaaatgcact ttagatgttt
    19141 catggaaatt gcggttacgg tattcgagat aatagggatt caactcaacg gaagccaaag
    19201 tttcttccgg cacaaaccct cctttcataa cgtctatgta aacaggatag gagctacttt
    19261 taacaccctt cgaaggcatc agatacaccc ccttcagcat ggaattgcta tcctggcgaa
    19321 attcttcggc aaagacttta tactcaaaac tgttgtgccc gaaaagaaaa ccgttttccg
    19381 gattttcatg ccgta
//
LOCUS       DAOSGL010000005        19111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_2325_length_19111_cov_4.603642, whole genome shotgun sequence.
ACCESSION   DAOSGL010000005 DAOSGL010000000
VERSION     DAOSGL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_19111_cov_4.603642"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2169
                     /locus_tag="PKY68_00350"
     CDS             <1..2169
                     /locus_tag="PKY68_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23523.1"
                     /translation="PKSHLGQFFLALLGFYIFLLALNCKPWLSMIGAIAYAFASYNLI
                     IIGAGHMNKGLVMATMAPILGGIILCYRKKFLWGGLITLIFTGLNILWGHQQITYYLI
                     LTIVILALVYLYYAFKEKQLKHFVLSSAVLLVLAGLAVLPALGFLIPSADYAKESMRG
                     GAVLQTDTEGKKESSGLEIDYAFAWSYGKAETMTLLIPNFYGGSSHYALGTDSECYKK
                     LRSTGQARQFCRAAPTYWGAQPFTSGPVYAGAIICFLFVLGLFIVKGPEKWWLLLATV
                     LAILMSWGHNFMAFNEFLFNHLPLYNKFRVPSMSLVIASLAMAALGILAVKELLDHRK
                     DAQFAQTYFKPLYLSFAIVGGLSLIIALFGSSMFDFSGNSDANFPAWLVEALKADRQQ
                     LLRSDAWRSFLYILLAFALIWFFIKKPFKEIYVILGLGLLITLDLWTVDKRFLNHSDF
                     VPKQKAKEILPSEADLQILQDKDPNYRVLNLTTSTFNDARTSYFHKSIGGYSGAKLRR
                     YQDIIDYHFSKGINTNVLNMLNTRYIILPSQEQQGKTVVQRNSQALGNAWFVEKITWV
                     DGPNAEIVALNDFNPAKEVFIDKAWQHLLPNSSTLEQTTDSSAYIRLSDYRSPGNLFY
                     ESNNPQAQLAVFSEVYYKTWQVFIDDERVPLLRVNYILRGLEIPAGQHIIEFKCVDEV
                     YERSAALSKWSSVIVGLLILGFTGFAGFKTIKKSTKRKSE"
     gene            2190..2966
                     /locus_tag="PKY68_00355"
     CDS             2190..2966
                     /locus_tag="PKY68_00355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH23524.1"
                     /translation="MPPKVSVITITYNAGEFLEKTLLSVLNQTSSDLEYVLVDGASTD
                     NTTDLIRQYEDLVNKGQFAIGAGQFRWISEADRGLYDAMNKGLKMARGEFVWFINAGD
                     KMYDNNSLELILNKLEDCPQSDVLYGQSLIIDQNDNALGERHKTAPAKLNKTSLLMGL
                     VVCHQSILVRRSLAPRYDLRYRISADYDWVCKVLSLSGQNTYIDAYISRFMVSGISAR
                     QRKKSLKERFHIMRKHFGLGATLGAHFILALRYPFTRKYH"
     gene            2968..3747
                     /locus_tag="PKY68_00360"
     CDS             2968..3747
                     /locus_tag="PKY68_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOH23525.1"
                     /translation="MKKIIGFFTARIPRQWLIRFSYIFSFLIRPFYIGNKVECPICES
                     RFRKFLPYGYGESADNRLCPKCLSLERHRLLWLYLKQKTLFFEAKLKVLHFAPEQPFL
                     RRFRALKNLDYTTADLDSPIADLKLDLCDIALPDNSYDVLLCNHVLEHVPDLDKALAE
                     IKRVLRPGGWAILLVPLNMNVNTFGDPSITDPKERQRLFGQYDHVRQFGKDYPDILRK
                     AGFLVTEDRLYYELSAWQRERQHLARAGEEIIYRCDKSLSL"
     gene            complement(3764..4699)
                     /locus_tag="PKY68_00365"
     CDS             complement(3764..4699)
                     /locus_tag="PKY68_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH23526.1"
                     /translation="MKRILVTGGAGFLGSHLCDQLLARGNEVICLDNYFTGSKQNVIH
                     LLDNPYFELLRHDITNPVYVEIDEIYNLACPASPVHYQYDAIKTIQTSVMGAINMLGL
                     AKRTEAKVLQASTSEVYGDPIVHPQPEEYWGNVNPIGIRSCYDEGKRCAETLFFDYYR
                     QNKVKIKVVRIFNTYGPRMHPNDGRVVSNFIVQALKGQDITIYGDGLQTRSFCYCDDL
                     IAGLITMMDTGDSVTGPINLGNPTEFTIKQLAELVIELTGTKSDIVYKPAVSDDPMQR
                     KPVIEKAKQVLNWEPRIQLREGLMRTIEYFDSILK"
     gene            complement(4706..5695)
                     /locus_tag="PKY68_00370"
     CDS             complement(4706..5695)
                     /locus_tag="PKY68_00370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH23527.1"
                     /translation="MNKELQLSIVVPVYNRPGEIGELLESLCAQTDPDFEVLIVEDGS
                     TLDCEEVCRQYSEKLQLNYLFKPNSGRSDSRNYGMERARGNYFLIFDSDCLLPPDYIQ
                     KVRTHLREHYLDCYGGPDKAAGDFTNRQKAINYAMTSFLTTGGIRGGDKKKASFTPRS
                     FNMGISKEVFRQVGGFKNIVGEDIDLSIRIKKAGFSTGLLSDAFVYHKRRVDLKSFYR
                     QVKTFGKGRVLISRMHPGSMKLLHLLPLIFVLAHVLLLLLSLVFLNPWFLLPLPVYML
                     ILFIDSLFKNKHAGIAALSVLAAYIQLFGYGLGLGNELLTKKAYTSTQEELYK"
     gene            5795..7321
                     /locus_tag="PKY68_00375"
     CDS             5795..7321
                     /locus_tag="PKY68_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH23528.1"
                     /translation="MGIIIRQSVKGTVVSYVGIAIGFFTTFFVLTNFLTAEEIGLTRV
                     LVDAATVFAGLAQLGTTSSAIRYYPYFKEGEKDHGFFFWTLVIPFIGFLIYGIVFWLL
                     KEPISGFFAENSPLFVDYYNFIFPVGFFLLYMSVFEVNSNLLLRIVVPKFIREVVVRL
                     LSLAAYLLYAFDLINLDGFIIAFSSVYGIATILNIVYIFSLKKISLKPDFKFLKKSLV
                     KDYLLYTLFLIAAALGGVIAPSINSFFISAKMGLNFAGIFAIAGYMASMIEMPYRSLG
                     TISGPHISQAIKDQDFTKINALCKSISLHQLLGGFFIFLAIWINIDLIFELLPNGEIY
                     AAGKWVVFILGLSRLLNSVFNVSEIVLRYSKFYYVSLILTVILTTSAIILNNSLIPVW
                     GMNGAAIASLLSYLIFYFLLLSLVRWKIKTSIFSWKQAQVVLLVLLLFLCDRLWTSFL
                     TPLFLKLPLKVIYAQVIDAIVKSSTLLGSGILLTYLWRISEDANALIRNILNRFRLRK
                     "
     gene            complement(7322..8557)
                     /locus_tag="PKY68_00380"
     CDS             complement(7322..8557)
                     /locus_tag="PKY68_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOH23529.1"
                     /translation="MYRLLFSLYVALFHIVALFYKKAGKIVKGQRDSWDILKNLNPKK
                     NYLWFHASSLGEFEQGRPLMEAITCTYPRYSVLLTFYSPSGFEVQKNYRGADAVCYLP
                     FDTMCNVRRFIELVQPRAAFFIKYEFWPHYLKELNKEGVPVYLVSGVFRKNQLFFKPW
                     GGFYRRLLRYFSAFFVQNETSKVLLNSIGVNNKVFITGDTRCDRVIDIAAHASEQELI
                     KEFAYDSMVLVAGSTWPEDEEILIPYINNVHTEWKLIIAPHEIDENRLRGIEKLLNRP
                     FVRYSAMKNEDAGRVECIIIDSFGLLSSVYRYGQAAYIGGGFGVGIHNTLEAAVYGIP
                     VLFGPNHTKFEEAQALIEAGAAFCISSQNDLEDELDKFLASNKTLTQAGENARNYVKE
                     KSGGTAKILDVLKTELFKV"
     gene            complement(8588..10111)
                     /gene="gltX"
                     /locus_tag="PKY68_00385"
     CDS             complement(8588..10111)
                     /gene="gltX"
                     /locus_tag="PKY68_00385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOH23530.1"
                     /translation="MSESNTVRVRFAPSPTGPLHIGGVRTALYNYLFARHAGGTMILR
                     IEDTDSKRYVPGSEAYIVEALQWLNIQIDEGVSQGGDYGPYRQSERKEIYKKYIDQLL
                     EQDKAYLAFDSPEQLEAKRAEDPNFQYDASSRLSMLNSLTLSATEVEERLQGGEDYTV
                     RFKIEPDRSIEVQDMIRGRVVFNSSVLDDKVLYKSADQLPTYHLANVVDDHLMKISHV
                     IRGEEWLPSTPLHVLLYEALGWDKQMPLFAHLPLLLKPDGKGKLSKRDGDRLDFPVFP
                     LNWEDPQSGEISKGYREAGYFPQAVINFLALLGWNPGDNREMFSMDQLIEAFTIERCS
                     KSGAKFDYEKARWFNHQYMQELDNSTLAESLLPLIEAKGYKTSPGFVEAILVHIKERM
                     VFPHDFWDQAAYFFAAPDQYDAKTVAKRWKPESAAQLTELVDLLERINDYSNPEAIES
                     EVKAWIEQKSYKMGLIMNAFRLALVGEGKGPGMFDIVCLLGKEETLQRLRKAIITLG"
     gene            10243..12336
                     /locus_tag="PKY68_00390"
     CDS             10243..12336
                     /locus_tag="PKY68_00390"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOH23531.1"
                     /translation="MAKFKPKLNTASTIFKIFLFVVAAGLILYAFPREGRFRYQFQEG
                     KPWRYGLLTAPYSFPVIKDQKELAAEKDSLLRTHKPYVVVDATVSTEMIGNFNRNYDL
                     YLRATVPPRYKTYINDQLALVYGAGIISASVRNEFQKEKLKSLLMVTKPIAETRDLSS
                     VFTIKEAYSAILSNLPARIHRDTLVRICKIEEYLRENLSYDRETSEQSKEAMIGELSE
                     TSGLVQRGERIIDKGEIVNAITFLKLSSLKKAAEERNINSTENWVLTGQIIWVAGLFL
                     MLFLFIRYFTPEIYNKTHNILFLLILVTSLSLLSALVVRNSTLNLYMIPYAILPVIIC
                     TFFNARTAYFTHLITVLICSVIAPFPFEFLTLQLTVGMAVVLSLKELTQRSQLFLCIV
                     WSFVCYSVFYTGIAFIQEGAPNQLQWQMYVLFAINAVLMFTAYLLIYLFEWIFGYTSN
                     VTLMELSNINSPLLRLFSESSPGTFQHSMQVSNLAAAAAQEINANAQLVRTGALYHDL
                     GKMQNPAYFTENQANGQNPLDQMETEKAAKIIVDHVNNGLKLANQYHLPKVVKDFIAT
                     HHGTGPTRYFYTIYKNEHPDQEPPKEAFYYPGPNPFSKETAILMMADTIEAASHSLTE
                     YSESSISDLVEKLIGRQIQEGAYKNVPITFQELEIIKSVFKQKLKSIYHTRIAYPEEK
                     KRAVRNTSDKRTNRK"
     gene            complement(12339..13304)
                     /locus_tag="PKY68_00395"
     CDS             complement(12339..13304)
                     /locus_tag="PKY68_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOH23532.1"
                     /translation="MKKNYSICVLDAFTSNPGDLSWDALRQFGEIQVYERSPEEKVIE
                     RAKDADILLTNKVILDEDLIKKLPRLKYIGVLATGYNVVDIHAAKAHNIVVTNIPAYS
                     TDSVAQMVFAHVLNIYNQVQYHAQEVKKGRWSGCRDFSFFDKPLMELKGKTMGIVGLG
                     RIGMATAQLALAFGMEVIALTSKTKDDLPAGISPVSEDELFRQSDVLSLHCPLTPENK
                     GFVQKEKLALMKTGAILVNLSRGPLVNEEDLAWALNNDRLYAAAVDVLSKEPPCPDNP
                     LLAAKNCYITPHIAWATKEARARLIAQAVENVRAFVEGHPINRID"
     gene            complement(13342..13935)
                     /gene="folE"
                     /locus_tag="PKY68_00400"
     CDS             complement(13342..13935)
                     /gene="folE"
                     /locus_tag="PKY68_00400"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOH23533.1"
                     /translation="MIDKRLILGTEPNPVQDEIAGHYLEILRLLGEDTEREGLLKTPK
                     RIAKAMQFLTYGYQLDPEEVLRLAKFHEEYRQMVIVRDIDFYSLCEHHMLPFYGKAHV
                     AYIPNQHITGLSKIARVVDIFSRRLQLQERMTTQIKECIQKTLNPLGVMVVIEAQHLC
                     MQMRGVEKQNSVAVTSDFTGVFNQAKTRQEFMNLIGR"
     gene            complement(13981..14502)
                     /locus_tag="PKY68_00405"
     CDS             complement(13981..14502)
                     /locus_tag="PKY68_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOH23534.1"
                     /translation="MLKTSDICRKMAFSLVVLFCCGTAQRLCAHRDSIDQSGERFIQF
                     LQTSEKNKGTVRIFQDRRIVDLVGSKQMSANIIKDGDKTLVSLNGWRIQIFSGNNQAT
                     AKEEAFSKEALVKAKLPDLNTYVIYNAPFWRVRVGDFFTYEEAYEVLLDLRRSFVFGR
                     EMSIVREKIQVEP"
     gene            complement(14685..15623)
                     /gene="mdh"
                     /locus_tag="PKY68_00410"
     CDS             complement(14685..15623)
                     /gene="mdh"
                     /locus_tag="PKY68_00410"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOH23535.1"
                     /translation="MSKVSVIGVGNVGATYASIMTINEVADEIVLLDVKEGIAEGKAL
                     DMKQASCLMSIDTRISGTTGDYSKTAGSDVVVITSGIPRKPGMTREELIGVNAGIVKD
                     VVSNVLKYSPDAILVIISNPMDTMTYLVQKTSGLPKNRIIGMGGVLDSSRFKTYLSMA
                     LGDVNLNDIQACVIGGHGDTTMIPLTRYATYKGIPVSEFLSEETLNKVAADTMVGGAT
                     LTKLLGTSAWYAPGAAGAYLVESILHDQKKVLPCSVYLEGEYGQKDICIGVPVVIGKN
                     GWEKVVDFKLNAAEQALFDKSAEAVRSTNKVLSSVL"
     gene            complement(15747..16976)
                     /locus_tag="PKY68_00415"
     CDS             complement(15747..16976)
                     /locus_tag="PKY68_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOH23536.1"
                     /translation="MKNVQLKKQGGAIWFLVVSTLLFAACGQKTTKPLSVRMAESEMI
                     RTPVPTDLDGCEGRLKWNYTHGLELLAFMDLYDASGDERFWNYAARYADTMINAQAEI
                     VSYKKTNYNIDHICPGRILFRMYDRTGEAKYKMAMDTLFSQLQTHPRTSDGGFWHKKV
                     YPYQMWLDGLYMGQPFYAEYISRYVDASNQQDLFEDVVHQFVTVGKHTYDAVTGVYRH
                     GWDESRAMFWADKETGQSAHCWGRGLGWYVMGLVDALDYIPGQEQRQALIDILKEIYE
                     VLPKYADPQTGMWYQVLEFPEREGNYQESTASIMFVYGLLKGVKTGVFDASYRETALQ
                     WYEKFVDRFVKENLDGTISITDCCAGAGLGGSQNRSGKYDYYINETIIIENDCKAVGP
                     FIQASLIYEEFKSERQQ"
     gene            complement(16986..17879)
                     /gene="rfbD"
                     /locus_tag="PKY68_00420"
     CDS             complement(16986..17879)
                     /gene="rfbD"
                     /locus_tag="PKY68_00420"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOH23537.1"
                     /translation="MKIMTRKVVLITGANGQLGSEIRLLENQYPQFAFTFTDIQELDL
                     QNSQAVFDCFSRLKPDYLINCAAYTAVDKAESDVELCYSINSAAVRNLALAAKQQNTI
                     VLHVSTDYVFDGKQARPYRENDAVCPVSVYGKSKLQGEQVLNEICPSSSVIVRTAWLY
                     SAFGNNFVKTMIRLGREKSELRVVNDQWGSPTYGADLAEALMKIVLSAEDGHFTPGIY
                     HFSNEGKCSWYEFAQKIHQLAGITNCKIIPVTTAEYPTAAQRPSFSLLDKSKIRRDYG
                     VAVPLWTDSLETCLSKFNNCI"
     gene            17907..18737
                     /locus_tag="PKY68_00425"
     CDS             17907..18737
                     /locus_tag="PKY68_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH23538.1"
                     /translation="MNFFSIDTGFFSTEAGAMFGIMSQKVWSKKYPADRENRSPLSMR
                     CLFVDFGERKVLFDTGVGNKQLRGMAYYRFRELIDIRDALKSKGYDAMQITDVVLSHL
                     HFDHCGGCTREGENGELELCFPRARHWASTAQWKNSLQPSSWEADAYLPENMQAVKDA
                     GKLHLIDKDQVLFPGLELKLYQGHTYDQIVSFIRTERGNLIFSGDVVPMATHIAQGCI
                     AAVDNHALLAVEEKMRLLEEACELQAGLVFYHDFYTKIAGLKKSNGRIWEAASPLALD
                     "
     gene            complement(18829..19020)
                     /locus_tag="PKY68_00430"
     CDS             complement(18829..19020)
                     /locus_tag="PKY68_00430"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOH23539.1"
                     /translation="MTVYIPFGLLGVDCKSLPSVLRANFYKCCSACSEKHYLSWSPVH
                     SAKPDFHRPEDFGFLFIGE"
BASE COUNT         5161 a         4492 c         4060 g         5398 t
ORIGIN      
        1 ccaaaatcac acctcggaca atttttttta gccctgctgg gattttatat ttttctgctg
       61 gctcttaact gcaagccctg gttaagcatg atcggagcca ttgcctatgc ctttgcctcc
      121 tacaatttaa ttattattgg agcaggacac atgaacaaag gcctggttat ggccaccatg
      181 gcacccatcc tgggaggcat tatactttgc taccggaaga aatttttatg gggagggttg
      241 attaccttga tatttactgg actcaatatc ttgtggggac atcagcaaat cacttattat
      301 ctcatcttga ccatagtcat cttggcgttg gtttacctgt attatgcctt taaagaaaaa
      361 cagttaaagc attttgtact ttcttctgcg gtattgctcg tactagcggg tttagccgtt
      421 cttccggctc tgggatttct tattccgtcg gccgattatg ccaaagaaag catgcgagga
      481 ggagccgtct tgcaaaccga caccgaaggc aaaaaagaaa gctccgggct ggaaatcgat
      541 tatgcttttg cctggagcta cgggaaggcc gaaaccatga ccttgcttat tcccaatttt
      601 tacggaggta gttcgcatta tgccttagga accgattccg agtgctataa aaaattgaga
      661 agtacaggtc aggccaggca attttgtcgg gcggctccca cttattgggg tgctcaacct
      721 tttacatcgg gacctgtgta tgccggcgcc attatctgtt tcctttttgt tctgggatta
      781 ttcatagtaa aaggccccga aaaatggtgg ctattgctgg ccaccgtttt ggctatattg
      841 atgtcctggg ggcataattt tatggctttc aatgaattcc tttttaatca tttgcctttg
      901 tacaataaat tcagggtgcc ctccatgtcg ctggttatag ccagtctggc catggctgcc
      961 ctgggaattc ttgccgtaaa agagctctta gatcatcgca aagacgcaca atttgcacag
     1021 acttatttta aacccttata cttatccttt gcaattgttg ggggattatc cctgattatt
     1081 gccttgtttg gaagcagtat gttcgatttt tcgggcaatt ccgatgccaa cttccctgcc
     1141 tggctggtag aggcactaaa ggccgacagg caacaattac tgcgttccga tgcctggcgc
     1201 tcttttctgt atattctgtt ggcctttgct ttgatctggt tctttatcaa aaaacctttc
     1261 aaagagatct acgttattct gggcttaggt ttgcttataa cactggattt gtggaccgta
     1321 gacaaacgct tcttaaacca cagcgatttt gtacccaaac aaaaagccaa agaaatactg
     1381 cccagcgaag ccgacttaca aattttacaa gataaggatc ccaattaccg agtgctcaat
     1441 ttaacgacca gcacttttaa cgatgcccgt acttcgtatt ttcacaaatc cattggaggt
     1501 tacagtggag ccaaactcag gagataccag gatataatcg attatcattt ctcgaagggc
     1561 atcaacacga atgtattaaa tatgctcaat acccgttata ttattctgcc ttcgcaggag
     1621 cagcagggta aaaccgtggt ccagcgaaac tcgcaggcat tggggaatgc ctggtttgtg
     1681 gagaaaatca cctgggttga cggccccaac gccgaaattg tcgccttgaa cgatttcaac
     1741 cccgccaaag aggtgtttat cgacaaagcc tggcaacact tactgcccaa cagctctacc
     1801 ctggaacaga cgaccgattc aagcgcctat attcgtctat ctgattatcg gagtcccggc
     1861 aacctgttct acgaaagcaa taatccccag gcacagttgg cagtcttttc cgaagtgtac
     1921 tataaaacct ggcaagtttt tattgacgac gaacgggtac ccttgttgcg ggtgaactat
     1981 attctgcgcg gactggaaat tccggccggc caacatatca ttgaatttaa atgcgtggat
     2041 gaggtttacg aacgaagcgc cgctttatcc aaatggtcgt ccgtaatagt gggattactg
     2101 atcctgggct ttacaggctt tgccgggttt aaaaccatca agaaatccac caaaagaaag
     2161 tctgagtaaa aagcaactct ctttgcaaaa tgccacctaa agtcagcgtc ataaccatca
     2221 cctacaacgc cggggagttt ttggaaaaaa ccctcctgag cgtactgaac caaaccagct
     2281 ccgatttgga atatgtactg gtagacggag cttctacaga caacacaacc gatctgatcc
     2341 ggcagtacga agacttggtg aataagggac aatttgctat cggggccggt caattccgct
     2401 ggatcagcga agcagaccgg ggtttatacg acgctatgaa caaaggcctg aaaatggccc
     2461 gtggagagtt tgtatggttt attaatgccg gcgataaaat gtacgacaac aacagcctcg
     2521 aactcatact gaacaaactg gaagattgtc cccaaagcga tgtgctgtac ggacaatcgc
     2581 tgattatcga tcaaaacgat aatgccctag gcgaaagaca caaaaccgct cccgctaaat
     2641 taaataaaac aagcctgctc atgggcctgg tcgtctgcca tcagtccatt ttggtacgta
     2701 gaagcctggc tccccggtac gacttgcgtt accgcatctc ggccgactac gattgggttt
     2761 gtaaagttct ttccctgtcc gggcaaaaca cctatattga cgcttatatt tcccgcttta
     2821 tggtgtcggg catttctgcc cggcagcgta aaaaatcctt gaaggaacgt tttcacatca
     2881 tgcgtaaaca ttttggactt ggtgctacac tgggagccca ttttatactg gcactgcgtt
     2941 atccctttac ccgaaaatac cattgacatg aagaaaataa taggcttttt tacagcccgt
     3001 atacccaggc aatggttgat acgcttcagc tatatcttca gttttctgat caggccattc
     3061 tatattggta ataaagtaga atgccctatt tgtgaaagtc gattcagaaa atttttacca
     3121 tatggttacg gggagagtgc cgacaaccga ctttgtccca aatgtttatc cttagagaga
     3181 catcgcttgt tgtggctcta tctgaaacaa aaaactttgt tttttgaagc taagctcaag
     3241 gttttgcact ttgcaccgga acaacccttt ctcaggcgct ttagagcttt gaaaaacctg
     3301 gactacacaa cggccgacct agactcgcct attgcagatc tcaagctgga tctttgtgat
     3361 atcgccttgc ccgacaacag ttacgatgtc ttgctatgca accacgtatt ggagcatgtc
     3421 cccgatctgg acaaggctct tgccgaaatc aaacgggtgc tgaggccggg gggctgggcc
     3481 attttgctgg ttccacttaa tatgaacgtt aatacttttg gcgatccgtc gataacagac
     3541 cccaaagaac gtcagcgtct gtttggtcag tacgaccacg tacgccagtt tggaaaagat
     3601 taccccgaca tactaagaaa agccggcttt ctggtgacag aagaccgact ttactacgaa
     3661 ttaagtgcct ggcagaggga aaggcagcac ctggccagag cgggcgagga aattatttat
     3721 cgttgcgata aatcactttc actttaatcg ttttccggag agtttatttt aagatggaat
     3781 cgaaatattc gatggtgcgc atcaaacctt ccctgagttg tatgcgcggt tcccagttga
     3841 ggacctgctt agccttttct ataacaggtt tgcgctgcat aggatcgtcc gaaactgccg
     3901 gcttgtaaac aatatccgat ttggtgccgg tcaattctat taccagttct gccagctgct
     3961 ttatggtaaa ttctgtaggg ttgcctaaat tgatggggcc ggttacggaa tctccggtat
     4021 ccatcatggt tatgaggcct gcaatcaaat cgtcgcaata acaaaaacta cgggtctgca
     4081 gcccatctcc gtatatggtg atgtcctgtc ctttcagtgc ctgcactatg aaattggaaa
     4141 ccaccctgcc gtcgttggga tgcattctgg gcccataggt attgaaaatc ctgactacct
     4201 tgattttgac tttattttgt ctgtaatagt caaaaaacaa agtttctgcg caacgtttgc
     4261 cctcgtcgta acaggaacga ataccaatgg gattgacatt gccccaatac tcttcgggct
     4321 gcggatgcac aatgggatct ccgtaaactt cgctggtaga agcctgtaag actttggctt
     4381 ctgtgcgttt tgccaagccc agcatattga tggctcccat cacggaagtc tgaattgttt
     4441 taatggcgtc gtattgataa tgtaccggcg aagcgggaca ggccagattg tagatttcgt
     4501 cgatttccac atatacgggg ttggtgatgt cgtgccgcaa caattcaaaa tagggattgt
     4561 ccagcaaatg gatcacattt tgcttggatc cggtaaaata gttatcaaga cagatgactt
     4621 cgttaccacg ggccaataat tgatcacaaa ggtgagaacc caaaaatccg gctccgccgg
     4681 taaccaatat tcttttcata acgtattatt tatacaattc ttcttgtgtc gatgtataag
     4741 ctttctttgt taaaagttca ttgccgagcc ccagtccgta gccaaacagc tgaatatagg
     4801 cggccagcac cgataaggct gcgatgcctg cgtgtttgtt tttgaacaag gagtcaatga
     4861 aaaggatgag catgtagacc ggcagaggca acaaaaacca gggattcaaa aaaaccaggc
     4921 tcagcaggag caagagcaca tgagccagga caaatatcag aggcaacagg tgtagcaatt
     4981 tcattgaacc cggatgcata cgggaaataa gcacccggcc tttgccaaaa gtttttacct
     5041 gcctgtaaaa gctctttaaa tctactctgc gtttatgata aacaaaagca tccgaaagta
     5101 aaccggtact gaaaccggcc tttttgattc gaatactcag atcgatgtct tcgcccacta
     5161 tgtttttaaa acctcccacc tgccggaaaa cttctttcga gattcccata ttaaaggacc
     5221 gtggcgtaaa ggaagccttc tttttatctc caccgcgtat accaccggta gtcagaaaag
     5281 aagtcatggc gtaattgatg gccttttgtc tatttgtaaa atcgccggct gctttgtcgg
     5341 gacctccata gcaatctagg taatgctccc gcaagtgagt tctgactttt tgtatatagt
     5401 cgggaggtaa gaggcaatcg gaatcgaaga ttaaaaaata attgccccgg gctctctcca
     5461 taccgtagtt ccggctgtcg ctccgacccg aattgggctt aaaaaggtag tttagttgca
     5521 gtttttcgct gtattgcctg caaacctctt cacagtcgag cgttgatccg tcttccacta
     5581 tcaggacttc gaaatcgggg tccgtctggg cgcacaagct ttcgaggagc tctccaattt
     5641 cgcccggtct gttgtaaacc ggaacaacga tagatagctg caattcttta ttcatgaata
     5701 cctaggtaga taatccgcaa aaataggaaa aaaaacaatc taagcgttta ttcgtctaaa
     5761 atacgtacat tcgcaacgga aacagagtgc atttatgggc ataatcatcc ggcaaagtgt
     5821 caaaggaacc gtagtgagtt atgtgggtat cgccatcggc tttttcacca ccttttttgt
     5881 ccttaccaat ttccttactg ccgaagagat agggctaaca cgtgtgttgg tcgatgccgc
     5941 cactgtattt gccggtcttg ctcaattggg caccacttcg tcggccattc gctattaccc
     6001 ttatttcaaa gaaggagaaa aagatcatgg attcttcttc tggaccctgg ttatcccttt
     6061 tatagggttt ttaatctacg gtatagtttt ttggttgctc aaagagccca tttcggggtt
     6121 ctttgccgag aattccccgt tgtttgtcga ttactacaac tttatctttc cggtaggctt
     6181 cttcttactt tacatgtctg tttttgaggt gaattccaat ttgcttctca gaattgtggt
     6241 gcctaaattt atcagggaag tagttgtcag gctattgtcg ctggctgcct atctccttta
     6301 tgccttcgat ctgatcaatc tggacggttt tattattgct ttcagctcgg tatacggcat
     6361 agcaaccatt ttaaacatcg tttatatatt cagccttaaa aaaatttctc tcaagcccga
     6421 ttttaagttt ttgaagaaat ccttggtcaa ggattatctc ctttacacct tatttttaat
     6481 cgcagcggcc ttgggaggcg ttatcgctcc cagcataaat tcttttttca tcagcgccaa
     6541 gatgggtttg aattttgccg gcatttttgc tattgcaggc tatatggctt ccatgattga
     6601 aatgccctac agatcgttgg gaaccatttc ggggccacac atctctcagg ccataaaaga
     6661 tcaagatttc acaaaaataa acgccttgtg taagagtatt tctttacatc agttgctggg
     6721 gggcttcttt atctttttgg ctatctggat taatatcgat ttgattttcg agcttttacc
     6781 caatggggaa atttatgcgg cgggaaaatg ggtggtattt atccttggac taagtcgatt
     6841 gttgaattct gtttttaatg tgagtgaaat cgtgttgcgc tactcaaaat tttactacgt
     6901 atcgctcatc ttaactgtta ttttgacgac cagcgcaatt attctcaaca attcactgat
     6961 accggtctgg ggcatgaacg gagctgcaat agcttctttg ttgtcgtatc tgatttttta
     7021 ttttttgttg cttagtttgg tacgttggaa aataaaaacc tccatttttt catggaaaca
     7081 agctcaggtt gtcttattgg ttttgttgct attcttgtgc gaccggttgt ggacgagctt
     7141 cctgaccccc ctatttttga aattacctct gaaggtaatt tatgctcagg tgatcgacgc
     7201 tatcgttaaa agctctacat tgctcggttc aggtatttta ctgacttatt tatggagaat
     7261 ttccgaggat gccaatgcgc tcataagaaa cattctgaac cgcttccggt tgaggaaata
     7321 actaaacctt aaacaattct gtttttaaga catccagaat tttggcagta cctcccgatt
     7381 tttcttttac ataattccgg gcattttctc cggcctgagt cagagtctta ttgcttgcga
     7441 gaaatttatc caactcgtct tccaaatcgt tttgagaact tatgcaaaaa gcggctcccg
     7501 cctctatcaa agcctgcgct tcttcgaatt tggtatgatt gggaccaaac aaaaccggga
     7561 taccgtaaac ggcagcttcc aaagtgttgt gtatgccgac tccaaaaccg ccccctatat
     7621 atgctgcctg cccgtaacga tataccgaag acaatagtcc aaaactgtcg ataatgatac
     7681 attcaacccg acctgcgtct tcgtttttca ttgccgaata gcgtacaaag ggccgattca
     7741 gaagcttttc tatcccccta agacgatttt cgtcaatttc atgcggagca atgattagtt
     7801 tccattccgt gtgtacatta tttatgtagg gaattagtat ttcttcgtct tcgggccagg
     7861 tactgccggc aaccaatacc atagaatcat aggcaaattc cttaatcagt tcttgttcag
     7921 aggcatgagc cgctatgtct ataacgcggt cgcagcgggt atctccggtg atgaagacct
     7981 tgttgttcac acctatagag ttaagtaaga ccttggaagt ttcgttttga acaaagaaag
     8041 cactgaagta tctaagcagt ctgcgataaa atccacccca aggtttgaaa aacaactggt
     8101 tcttgcgaaa cacgccggaa acaaggtaaa caggaacgcc ctctttattt aattctttca
     8161 gataatgggg ccagaattca tacttaataa aaaaggcagc tctgggctgt accaattcta
     8221 taaaacgcct gacgttacac atcgtatcga aaggcaggta acaaaccgca tcggctcccc
     8281 ggtagttttt ttgcacctca aaacccgagg gtgaataaaa agtcaacaag acagaatacc
     8341 gtggatacgt acaggtaata gcctccatca aagggcgtcc ctgttcaaac tcgcccagcg
     8401 aagaggcatg aaaccaaaga taattctttt tgggattcag gtttttcaaa atatcccaac
     8461 tgtctcgttg gcctttgact attttaccgg cttttttgta aaaaagcgca actatgtgga
     8521 acaaagctac ataaagcgaa aaaaggaggc gatacataat aagcagatta attaacaaca
     8581 aatcgtacta gcccagcgtt attatggctt tacgtaaacg ctgtagcgtt tcttctttcc
     8641 ccagcaggca aacgatgtca aacatgcccg gacccttgcc ttcgcccact aaagccaaac
     8701 gaaaagcatt cataatcaaa cccatcttgt atgatttttg ttctatccag gcttttactt
     8761 cgctttcgat ggcttcggga ttggaataat cgttgatgcg ttcgagcaaa tcgaccaatt
     8821 cggtaagttg agctgcagat tctggcttcc aacgcttggc caccgtctta gcatcgtatt
     8881 gatcgggggc ggcaaaaaaa taggcagcct gatcccaaaa atcatgcgga aaaaccattc
     8941 tttccttaat gtggaccaaa atagcctcga caaaaccggg agatgttttg tatccttttg
     9001 cttctatcaa gggtaacaag ctctctgcca aggtactgtt gtccagctcc tgcatatatt
     9061 gatggttgaa ccagcgagct ttttcgtaat cgaacttggc tccgcttttg ctgcaacgtt
     9121 ctatggtaaa agcttctatc aattggtcca tcgaaaacat ctccctattg tctccgggat
     9181 tccagcccaa caacgcgaga aaattgatga ctgcctgggg gaaatagccg gcttcgcgat
     9241 aacctttgct tatttctccg ctttgaggat cttcccagtt taagggaaaa accggaaaat
     9301 ccagacggtc tccgtcacgc ttgctcagtt tccctttgcc atcaggttta agcaacaagg
     9361 gtaaatgagc aaagaggggc atttgtttgt cccaaccaag ggcttcgtac agtaaaacat
     9421 gcaaaggggt ggagggcagc cattcttctc ccctgatcac atggctgatt ttcatcaaat
     9481 gatcgtccac cacattggcc aggtggtagg tgggcaattg atcggccgac ttgtacaaga
     9541 ccttgtcgtc cagaacactc gaattaaaaa ccacccttcc tctgatcata tcttgcacct
     9601 ctatgctacg atcgggttct atcttgaaac gaacggtata atcttcacct ccctgcaatc
     9661 tttcttcgac ctcggtggcg gacaaagtaa gcgagttgag catgcttagt cggcttgaag
     9721 catcgtattg aaaattaggg tcttctgctc gttttgcctc aagctgttcg ggagaatcaa
     9781 aagccagata ggccttgtct tgttcgagca attgatctat gtattttttg taaatttctt
     9841 tccgttccga ttgacgataa gggccatagt cgcctccctg cgaaacaccc tcgtcaattt
     9901 ggatattgag ccattgcagt gcttccacaa tatatgcttc ggatccgggt acataacgtt
     9961 tgctgtcggt atcttctatg cgtaaaatca tggtgccgcc ggcgtggcgg gcaaataaat
    10021 aattataaag agcggtcctt acgcctccta tgtgcagagg accggtaggg ctgggagcaa
    10081 aacggacacg gacagtattg gattcagaca taacacaaac attttgaggg caaaagtaca
    10141 agatattttt aaaattcggc cttagttatt gccgccaagg gttttttttg taattttcgc
    10201 ccctgttcac aggcttccgg ccgaaaaatc agattgaaag ccatggctaa atttaaacct
    10261 aaactaaata ctgcatccac gatattcaaa atcttccttt tcgttgttgc agccggcctg
    10321 atcctttacg ctttccccag agaaggcaga ttccgctatc agtttcaaga aggcaaacct
    10381 tggcgctacg gcttgctcac ggctccctac agtttccccg ttattaaaga ccagaaagaa
    10441 ctggctgccg aaaaggacag cctgttgagg acgcacaaac cttacgttgt agtggatgca
    10501 acggtctcaa cagaaatgat tggtaacttc aaccgcaatt acgacttgta cttaagagct
    10561 accgtacccc cacgttacaa aacttatata aacgatcaac ttgccctggt ctatggagcc
    10621 ggtatcattt cggcttccgt ccgaaacgaa ttccaaaagg aaaagttgaa atcgctgctg
    10681 atggttacta agcctatagc cgaaacccgc gatctgtctt cggttttcac tattaaagaa
    10741 gcctattctg ccattttgag caacttgccg gcacgtatac atcgagatac cttggtcagg
    10801 atatgcaaga tagaggaata cctccgggaa aacctaagct atgatcggga aacttctgaa
    10861 caatcgaaag aagcaatgat tggtgagctg tctgaaacct cgggattggt ccagcgagga
    10921 gaacgcatca ttgataaagg cgaaattgtt aacgcaatca cttttttaaa actttcttcg
    10981 ttaaaaaagg cagcagaaga acgtaatata aattcaacgg aaaactgggt gctgaccggc
    11041 cagattatat gggtagccgg cctgtttttg atgctgttcc tatttattag gtattttaca
    11101 cccgaaatct acaacaaaac ccacaacatc ctctttttac tgattcttgt cacaagtctt
    11161 agtttattga gcgccttagt ggtgagaaac agcaccttga atctctatat gattccctat
    11221 gccatactgc ctgtgataat ctgtaccttc ttcaatgcca gaacggctta tttcacccat
    11281 ctcatcaccg tattaatctg ttctgtcatt gctcccttcc cctttgagtt tttaacgctg
    11341 caactcactg ttggtatggc tgttgtcctc tcactcaaag agcttactca gcgttcacaa
    11401 ttgtttttgt gtatcgtctg gagttttgtc tgctattcgg tattttacac cggcatagct
    11461 tttattcaag agggagcgcc caatcaactt caatggcaaa tgtatgtcct ttttgccatt
    11521 aacgcagtac tgatgttcac cgcctacctc ctgatatatt tgtttgaatg gatattcggc
    11581 tatacttcca atgtcactct aatggagctc tccaacatta actccccctt gctccgcttg
    11641 ttttcagaaa gctctccggg cactttccaa cattccatgc aggtttccaa cctggctgcc
    11701 gccgccgctc aggaaataaa cgccaatgca caactggtaa gaacaggggc cttgtatcac
    11761 gacctgggca aaatgcaaaa tcccgcctat tttaccgaga accaggcaaa cggacaaaat
    11821 cccttagacc aaatggaaac agaaaaagca gccaaaataa tagtggatca tgtcaacaat
    11881 ggtctcaaac tggccaatca ataccacctg cccaaagtgg tgaaagactt tattgcaact
    11941 catcacggta ccggaccgac acgctatttc tacactatct acaaaaacga acatcccgac
    12001 caggagcctc caaaagaagc attttattat ccgggcccaa atcctttttc taaagagaca
    12061 gctatcctaa tgatggccga tactattgag gccgcttcgc atagtttaac ggaatattcc
    12121 gaaagcagca tttcggatct ggtagagaag ctgatcggta gacaaattca ggaaggggct
    12181 tataaaaatg tacccatcac cttccaggaa ttggagatca ttaaatcggt ctttaaacaa
    12241 aaattaaaaa gcatttatca tacacgaata gcctatcctg aagaaaagaa gagggctgtt
    12301 cgtaacactt cggataaaag aactaacagg aagtagaatt aatcgattct gttaatcgga
    12361 tggccttcca cgaaggctct gacgttttcg actgcctggg caatcaaacg cgcccgggct
    12421 tctttggttg cccaggcaat gtgaggggtg atgtaacagt tcttagctgc cagtaagggg
    12481 ttgtcggggc atggaggttc tttagataat acatcaacag ctgctgcata gagtctgtcg
    12541 ttattcaaag cccaagcaag gtcttcttcg ttaaccaatg gtccccggct taaatttacc
    12601 agtatagctc ctgttttcat taaagccaat ttttcctttt gcacaaagcc cttgttttcg
    12661 ggtgttaggg ggcaatgcag gctcagcaca tcgctttgtc gaaacaattc atcttcactg
    12721 accggagaaa taccggcagg taaatcgtct ttggtcttgc ttgtcagggc aataacttcc
    12781 attccaaaag ccagggccaa ttgggcagta gccattccta ttctacccaa acccacaata
    12841 cccattgttt tgcctttgag ttccatcaaa ggtttatcga aaaaagaaaa atcacggcag
    12901 cccgaccaac ggcctttttt cacttcctgc gcgtgatatt gtacttggtt gtatatgttc
    12961 agtacgtgag caaacaccat ctgagccacc gaatcggtgc tgtaagcagg aatattggtc
    13021 accactatgt tgtgggcttt ggcagcgtgt atgtcaacca cattataacc ggtagccagc
    13081 actccgatat acttaagcct tggcagtttt tttatcagat cctcgtccag aatcactttg
    13141 ttagtcaaca aaatgtcggc atctttcgcc cgctcaataa ctttttcctc gggactgcgc
    13201 tcgtacacct gtatttctcc aaattgacgg agagcatccc aggacaagtc tccgggattg
    13261 gaggtaaaag cgtccaacac gcaaatggaa taattctttt tcatggcctt ttattcaaca
    13321 gcaaactgta ttggagctac tttaccttcc aatcagattc ataaattcct gcctggtctt
    13381 tgcctggtta aagacaccgg taaaatcgga ggtgacagca actgaatttt gtttttcaac
    13441 tccccgcatt tgcatacaaa gatgttgtgc ctcaatcaca accatcaccc ccagaggatt
    13501 gagggttttc tgaatgcatt ctttgatctg cgtagtcatg cgttcctgca actgcaagcg
    13561 gcgtgaaaaa atatccacca cccgggcaat cttactcagg cctgtaatat gctgattggg
    13621 aatataagcc acatgggctt taccgtaaaa gggcagcatg tggtgttcgc acaaggaata
    13681 aaaatctata tccctgacga taaccatttg ccggtattcc tcatgaaatt tggccaatct
    13741 taacacttct tcgggatcca actgataacc gtaagtaaga aattgcatgg ctttagctat
    13801 gcgcttaggt gttttcaaca aaccttcacg ttccgtgtct tctcccagca aacgtaaaat
    13861 ttccaagtaa tgtccggcta tttcatcctg aacaggatta ggttcagttc caagaattaa
    13921 tcgtttatct atcatagact tagttcttta tcccgacttt aagtataata gctttctcag
    13981 ctaaggttca acctgtattt tttcgcgaac tatcgacatt tcgcgtccga acacgaaact
    14041 ccttctcaga tcgagtaaga cctcataagc ttcttcgtaa gtaaaaaaat ctccgacacg
    14101 caccctccag aaaggagcgt tataaatgac atatgtgttc aaatcaggca gtttggcctt
    14161 cactaaagcc tccttgctga aagcttcttc tttggcagta gcctgattat taccagaaaa
    14221 tatttgaata cgccagccat tcaagctgac cagggttttg tctccgtctt tgattatgtt
    14281 ggccgacatt tgcttactgc caactaagtc tacaatgcgc ctgtcttgaa aaatccgtac
    14341 cgtgcccttg tttttttcgg aagtttgcaa aaactgtata aaacgttcgc cggattgatc
    14401 gatgctatcc cgatgggcac acaacctctg ggccgttccg caacaaaaca gcacaacaag
    14461 gctaaacgcc attttccggc agatatcgga tgttttcagc attttaaaaa ttaaaagggg
    14521 atgtctcaaa attaactatt caagcacaaa acataaggtg tcttagaagc gtccaaatgc
    14581 aagggctgag attgagggca aaggagcata ctgaagtatg tgactgagcc tgaatatcga
    14641 aagcaacgca gcagttgggc acttatgaga caccgtcgat aaaattacag tacagagctc
    14701 agtactttgt tggtagaccg gacagcctca gcgcttttat cgaacaaagc ctgttccgcg
    14761 gcgttaagtt tgaagtccac gactttctcc cagccgtttt ttccaataac taccggaaca
    14821 ccaatgcaaa tgtctttttg cccgtattcg ccttcgaggt aaacgctgca tggcaacacc
    14881 tttttctggt cgtgcaaaat ggattctacc aggtaagctc cggcagctcc tggagcatac
    14941 caggccgaag tgcccagcaa tttggtaagt gttgctccac caaccatggt gtcggctgca
    15001 accttattca aagtttcttc gctaagaaat tccgaaaccg gaatgccctt gtaggtggcg
    15061 taccgggtaa gaggaatcat ggtggtgtct ccatgtccgc caatcacaca agcctggatg
    15121 tcgttcaggt taacatcacc cagagccatg ctcaaatagg ttttaaaacg ggaactgtcc
    15181 aaaactcctc ccattccgat aattcgattt ttaggaaggc ccgatgtttt ctggaccagg
    15241 taagtcatag tgtccatggg attactgatg ataaccagta tggcatcggg agaatatttc
    15301 aagacattgg atacaacgtc tttcactata ccggcattaa caccaataag ttcttctctg
    15361 gtcattccgg gcttacgggg aatacccgaa gtaatcacta ccacatcaga accggccgtt
    15421 ttagaataat ctcctgtagt acctgaaatt ctggtgtcta tactcatcaa acaggatgct
    15481 tgtttcatgt cgagagcttt gccctcggca atcccttctt taacgtccaa gaggacaatt
    15541 tcatctgcta cttcgttgat ggtcattatg ctcgcataag ttgcacctac atttcctact
    15601 cctattacac ttacttttga cataaaattt agatctaaat taacttatta tcctattaaa
    15661 aactatcctt ataaaaggat tggcaaagat acaatttgtt tttgctttca aagctctaag
    15721 tcgcaacttt ttggatgctc tgaactttat tgctgtcttt cggacttgaa ttcctcgtat
    15781 atcaacgaag cctgaataaa tgggcccacg gccttgcagt cgttttcgat gataatggtt
    15841 tcgttgatgt aataatcgta ttttccactg cgattttgtg aaccgcccag acctgcaccc
    15901 gcacaacagt cggtaatgct gatggtcccg tcgagattct cttttacaaa acgatccacg
    15961 aacttctcgt accattgcag cgccgtttca cggtaagagg catcaaaaac tcctgtttta
    16021 acccctttga gcaggccata cacaaacatg atggatgcgg tagattcctg ataattaccc
    16081 tccctttcgg gaaactccag aacttgatac cacatgccgg tttggggatc tgcgtatttt
    16141 ggcagcacct cgtatatctc cttgagaata tcgatcaaag cctgacgctg ttcttgtccc
    16201 ggtatgtaat ccaaagcatc caccaggccc attacatacc agcccaaacc tctgccccag
    16261 caatgggccg attgtcctgt ttccttatct gcccaaaaca tagcgcggct ttcatcccag
    16321 ccatgcctgt acactccggt gacagcatcg taggtgtgtt tacccacagt gacaaactgg
    16381 tgaacaacgt cctcaaataa gtcttgctga tttgaagcat cgacatagcg gctaatgtat
    16441 tccgcataaa aaggctgccc catgtacaaa ccgtccagcc acatttgata aggatacact
    16501 tttttatgcc agaaacctcc gtcggaggtg cgtgggtgtg tttgcaactg cgaaaacaag
    16561 gtgtccatag ccattttgta cttagcttct ccagttctgt cgtacatgcg gaacaaaatc
    16621 cggccggggc agatatggtc gatattatag ttggtctttt tataggaaac aatctcggcc
    16681 tgcgcgttga tcatggtatc ggcataacgg gcggcataat tccagaaacg ctcatctcca
    16741 ctcgcatcgt ataaatccat aaaagccagc aactccaatc cgtgcgtata attccatttg
    16801 agccgaccct cgcatccgtc caaatcggtt ggtacgggag tcctgatcat ttcgctttcg
    16861 gccatgcgaa ccgaaagagg tttagtggtt ttttgtccac aggcggcaaa taaaagcgtg
    16921 cttacaacaa gaaaccatat cgctccgcct tgttttttta attgaacatt tttcattgta
    16981 cagatttaaa tacagttatt aaatttcgac aaacaagtct ccagactgtc tgtccacaag
    17041 ggaacagcca ctccgtaatc tctgcggatt ttgcttttgt ccagcaggct gaaggagggc
    17101 ctttgggccg ccgtaggata ttcggcagta gtcaccggta ttattttaca atttgtaatt
    17161 ccggccagtt gatgaatctt ttgagcaaat tcgtaccagg aacattttcc ctcgttactg
    17221 aaatgataga taccaggggt aaaatgcccg tcttcggcgc ttaatacgat tttcatcaaa
    17281 gcctccgcca gatcggcccc ataggtaggg ctgccccatt gatcattcac cactctgagt
    17341 tccgattttt cccgccccaa acgaatcatg gtctttacaa aattgtttcc aaacgcagaa
    17401 taaagccaag cggtacgaac tatcaccgaa gaagaaggac agatttcatt caacacctgc
    17461 tccccctgta atttactctt gccatagacc gaaacaggac aaaccgcatc gttttcgcga
    17521 taaggcctgg cttgtttccc atcaaaaaca tagtccgtgg aaacatgcaa aacaatagta
    17581 ttctgttgct tagcagctaa ggcaaggttt ctgactgcag cactgtttat ggaataacac
    17641 agctctacgt cactttctgc cttatctaca gccgtataag cggcacaatt gattaaataa
    17701 tccggcttca accggctgaa gcagtcaaaa actgcttggg agttttgcaa atccaactcc
    17761 tgaatgtcgg taaaagtgaa cgcaaattga ggatattgat tttccaacaa tcttatttca
    17821 ctacccaatt ggccgttggc tccggtaata agaactactt tccgcgtcat aatttttata
    17881 actttgtcgc aaaattaggt aaatatatga acttcttcag catagacacg ggcttttttt
    17941 cgaccgaggc aggagccatg ttcggaatca tgtcccaaaa agtctggtca aaaaaatacc
    18001 cggccgaccg ggaaaatcgc agtcctttga gtatgcgttg cctttttgtt gattttggcg
    18061 aacgaaaagt gctctttgat accggggtgg gaaacaaaca actcagaggt atggcttatt
    18121 accgctttcg cgaactgatc gacatcagag atgccctaaa aagcaaaggc tacgatgcca
    18181 tgcaaatcac cgatgtggtg ctgagtcatc tgcactttga ccattgcgga ggctgcacaa
    18241 gggaagggga gaatggagaa cttgaacttt gctttcccag ggcccgacac tgggcaagca
    18301 cagctcagtg gaaaaattcc ctgcaaccct ccagttggga agccgatgcc tatctgcccg
    18361 aaaacatgca ggccgtgaaa gatgccggca agttacatct tatcgacaaa gaccaggtct
    18421 tgtttccggg tctggaactt aaactctatc aaggtcatac ctacgatcag atagtctcct
    18481 ttatccgcac ggaacgtggt aatcttattt tctccggcga tgtggtccca atggctaccc
    18541 atattgcaca gggctgtatt gcagccgtgg acaatcacgc cttactggcc gtggaagaaa
    18601 aaatgcggct tttggaagaa gcctgcgagt tacaggccgg actggtcttt taccacgact
    18661 tctacaccaa aattgccggt ctcaaaaaaa gcaatggccg tatttgggag gcagcttcgc
    18721 ccctggcttt ggactgacag ataatctata ttgtccgagt acaatctcta aagatagctt
    18781 acagatttgt ttggctccga gctatgttat cgactcacca cactgttact actctccgat
    18841 aaataagaat ccaaaatcct ccggacggtg aaaatcaggt tttgctgaat gaacagggct
    18901 ccagctcagg taatgttttt cggaacaggc cgaacaacac ttataaaaat ttgctcgcaa
    18961 aacagaaggc aaactcttgc aatcgacacc taataaccca aagggtatat ataccgtcag
    19021 agaccaagcc tgaaccggct tgtcaacggc cctcgggaag agtccggaag gcttgtcggc
    19081 tctctccgga aaaaattcgg ctgtctcgtc a
//
LOCUS       DAOSGL010000006        17857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_2560_length_17857_cov_4.633637, whole genome shotgun sequence.
ACCESSION   DAOSGL010000006 DAOSGL010000000
VERSION     DAOSGL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17857
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2560_length_17857_cov_4.633637"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1029)
                     /locus_tag="PKY68_00435"
     CDS             complement(70..1029)
                     /locus_tag="PKY68_00435"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HOH23540.1"
                     /translation="MKIIGTGSSSPSLTVSNDMLATFLDTSDEWIQTRTGIKTRQVIS
                     TERLEDLAAQACSIAIHEAGIKAEDLDYIICSNTLNEYVTPALSCILQGMLGADCPCI
                     DLNGACAGFIYALDMADALFERGNMEHILVVCAEEPTRMVDWTKRDTCVLFGDGAGAV
                     VLGKGEGFKSFKLSTFSNTEALYQIRKLQDTPFIKREETEGNGALVMKGQDVFKLAVN
                     CSLNDIKDVLKLAGVKADEIDHYLLHQANMRILDTIMRYLGQSEKNFPHNIERYGNTS
                     SASIPMLLDELNKNNKLKTGQKLVFSAFGAGFTTGACLLEWTM"
     gene            1418..2083
                     /locus_tag="PKY68_00440"
     CDS             1418..2083
                     /locus_tag="PKY68_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOH23541.1"
                     /translation="MSRILVFNSKKWFKLTFLGFFVWLFAEIVLITTLYTFVTITFIE
                     QTDASFWTVFPRALLYTSVILIIPDTISVLYCALNDKNRTLRLLNYKDFVSDDELANK
                     DKELIHLTDNYGNLKLSIKLDNLYYIESHDNYIKVYYMNKGAMCNYLLRCKLKTIEES
                     FANSSLMRCHRSYIINSDKVKVIRKEKEGIYIDLDSEKVKSIPVSKGYSQKVMRYFGS
                     DSV"
     gene            complement(2186..4021)
                     /locus_tag="PKY68_00445"
     CDS             complement(2186..4021)
                     /locus_tag="PKY68_00445"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOH23542.1"
                     /translation="MSKIQAKEAWGTRLGLILAMAGNAVGLGNFLRFPIQAIQNGGGA
                     FIIPYLVSFVLLGIPLMLVEWSTGRYAGLRGHHSSPFVLQSLDKRPYWKYIGVIGLFS
                     NIVIASYYCYIESWTLSYVYHSIIRTFDGMTEIQVSDFFDDYLRISTSTTGIPYESIL
                     FYILCLSINVWILSQTLRKGIERAAKFFMPLLIIFGLFLVVKIVTIKAGEQGAIYDGW
                     VGLEFLWTPDLNSLSDPKVWLSAAGQIFFTLSLGMGCIQCYASYLKENDDVVLNSMTA
                     GFMNEFVEIVIGGSIIIPISIGYFGIDKVMDLCQIGGFGLGFRTMPYLFEQWGDVLAS
                     LAGIAFFGLLFGAGITSSLAMGAPIVGFLKDNFGWSRKKGALAFGSIILAFGIPTVLF
                     FSKGVFDEYDYWGGTVALVLYATAEIILFSWIFGVNRGWELIHQGADMRMPGIFKFIL
                     RYITPTMLILILLASAVKPKNDDWSLLSLQGWELDKNSIIGELSHKDIGPNNTWFAKE
                     YFAERDGLVHKITATEKGNMLEILSANTIKSYHLPSRHTLMVSEGDRIKAGDVLYKGK
                     IVNTVFYIDSARIGLLILFLMLCFFVWKAANKKSHNYFDIEQLNQ"
     gene            4199..6439
                     /gene="pnp"
                     /locus_tag="PKY68_00450"
     CDS             4199..6439
                     /gene="pnp"
                     /locus_tag="PKY68_00450"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOH23543.1"
                     /translation="MMTPIVKTIELGDGRTITIETGKLAKQADGSVVVRMNNIVLLAT
                     VCADKEAVPGTDFMPLQVEYREKFASSGRFPGGFLKREGRPSDSEILTSRLVDRALRP
                     LFPADFHANTIVNVILFSADNDCNGDMPDALAGLAASAALAVSDIPFNGPISEVRIAR
                     IDGQFKLNPTSAELKKADMDIMVGATLENIMMVEGEMSEVQESDLLEALKIAHEAIKA
                     QCRAQLELAELAGKTVKREYCHEENDEELRKDIWDKTYAKAYAEATSANPDKHQREAN
                     FKAIYEEYIAAMDEETALEKAEMISRYYHEVEKEAMRRSILDEGKRLDGRTTTEIRPI
                     WSEIDCLPGCHGSAIFTRGETQSLTSVTLGTKLDEKIVDDALNQSTERFLLHYNFPPF
                     STGEARASRGVGRREIGHGNLAHRALKRMLPEEYPYVIRVVSDILESNGSSSMATVCA
                     GTLALMDAGVPIKKPVSGIAMGLITDNKKFAILSDILGDEDHLGDMDFKVTGTKDGIT
                     ATQMDIKVDGLPYEILEKALMQAREGRMHILGKITETMPAPRPELKPNAPRIELMWIP
                     KEFIGAVIGPGGKIIQGMQEETGAIITIDEIDNQGRVEIAAVNKASIDAAIMKIKSIV
                     AMPEAGEVYRGKVRSIMPYGAFVEFMPGKDGLLHISEITWKRLEAIEEAGLQEGDEID
                     VKLLDIDPKTGKFKLSHKALLEKPEGYEERPERERRPRPDKRQGGDDAGRGRGGRRRP
                     DSNRDR"
     gene            6458..6706
                     /locus_tag="PKY68_00455"
     CDS             6458..6706
                     /locus_tag="PKY68_00455"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOH23544.1"
                     /translation="MKKDQRKSVVLRGYNTATEANIVKSMLESNGIACFLSNETSVFT
                     PGFFSASSTVLLHVMEEDIEKASSLIDQIPEAFSEEES"
     gene            complement(6719..8002)
                     /locus_tag="PKY68_00460"
     CDS             complement(6719..8002)
                     /locus_tag="PKY68_00460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH23545.1"
                     /translation="MKFEELDLEEELLDGLWAMNFRECTPVQELAIPVLLEGNDLIGC
                     AQTGTGKTAAYLLPILNNLVLDKHAHDAIRAVIMVPTRELAQQIDQQVEGFAYYLPVS
                     SIAVYGGNDGMVWEQQKKSLDMGVDIVIATPGRLLSYIKLGLIDLSQVKYFILDEADR
                     MLDMGFIDDIMLIIKELPTKRQTMLFSATMPPKIRELAKNIMHQPKEIRLAVSKPNEN
                     IDQSAYICYERQKPELVNILFSSMQTGEKVLIFASSKIKVKEVRRILKRLNLSVIEMH
                     SDLEQAEREKVMLSFKTGKVNILVATDIVSRGIDIDDIALVINYDVPHDPEDYVHRIG
                     RTARAGEKGRAVTFVSEQDQYKFLQIERFLEKEVVKNTLPGKLGEGPPYQPQKARRSR
                     FVNKVKSENKKATTRGRNKKSGSRKPFKGGARSKS"
     gene            8094..10994
                     /locus_tag="PKY68_00465"
     CDS             8094..10994
                     /locus_tag="PKY68_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH23546.1"
                     /translation="MKTIKFLSLAWLLILLFSACDRGYECVPGDPMNTRIYTLDNGLK
                     VYMTVNKDEPRIQTYIAVKVGAKNDPAETTGLAHYFEHLMFKGSKQFGTMNYELEKPI
                     LDRIEALFEEYRSKTDEAERKAIYRTIDSLSYEASRFFIPNEYDKLMTAIGASGTNAY
                     TGFDMTVYTEDIPSNQIENWAKIQADRFQNNVIRGFHTELETVYEEKNMSLTNDSRKV
                     YEKVLAILFPHHPYGTQTVLGTQEHLKNPSITNIKNYYSTYYVPNNMAICLSGDFNPK
                     EMIQVIKKYFGEMKPAVSIPELEYEEGKALSEPVVCEVLGNDAENVTLGWSCVGIGDE
                     EFNLLTLTDMILSNGKAGLIDLDLIQQQKVLNAFSGAYEMADYSAYVIQGRPKEGQSL
                     EEVKDLLLEEMAKLKAGAFDDYLLEASINNLKLSRIREMQSNNGRADMYVRAFINELD
                     WADEVKRMDDLSQISKEDVVAFANKWFNDGYALVYKRQGIDPNEKKIAKPEITPILTN
                     RDTVSAFLKEIQQTIVKPIQPVFLDFKKDFSRFMVQADLPVLYIQNQTDDLFELTYWF
                     DMGTNTNKLLSLAFSYLNYLGTSEFTAEQIKSEFYKMACSYNLSVGSEKAQIRLTGLG
                     ENMPEALNLLEKLLFDAQVNQQAFDNMIQDVLKSRANAKLSQSRIFNMLRNYAVYGPG
                     SPATNVLSDQELLNLKPEELIEVLNKLTSYSHKVLYYGPDSKEQLTSVLKEKHLVPET
                     LFELPAPKEFPYLLTENNQVLFAQYNAKQLYLSMYSNNGEVFDLQKEPLTTLYNEYFG
                     GGMNSIVFQEMREARGLAYMAAAAYVTPSRAGRPYSMVSIIATQTDKMDDALQAFNQI
                     IEDMPESEAAFKLAKDGLFSRLRTERILRSNILWAYLDALDYGLEEDSRKYLYESLTD
                     LNLEDLKNFQQQWIKNRSYSYCVLGDEKEVDFDNLAQYGPVTKLELETLFGY"
     gene            11115..11945
                     /locus_tag="PKY68_00470"
     CDS             11115..11945
                     /locus_tag="PKY68_00470"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOH23547.1"
                     /translation="MLKYLCATIFTMGVICSCTRIEGDGVLVKAMRSSSSFSEVDSRG
                     EFEVYLYYADSCKVEVECESNILQYIVTKFVRNKLVVDKKNNVLIANNMPIKVYASTP
                     SLKFAELSGSGRMVVDELHSGNLTLSLSGSGEIIYKDSVECESLTMDISGSGKINAPY
                     VKSGQTNIDIDGSGVCTVDSISSEKLDIDIDGSGGINLSGRCDYVDLRIAGTGKLEAS
                     DLLTKRYDIRVNGSGCAYINVSNELNIIVNGSGNIYYLGYPKITMTGSGNGGLINMNP
                     "
     gene            complement(11949..13469)
                     /gene="guaA"
                     /locus_tag="PKY68_00475"
     CDS             complement(11949..13469)
                     /gene="guaA"
                     /locus_tag="PKY68_00475"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOH23548.1"
                     /translation="MQEKIVVLDFGSKTTQLIARRIRELKAFCEIYPYNKFPENTDGI
                     KGVILSGSPFSVYDKNAFDFDLKLVRKKFPLLGICYGAQLIVHKAGGKIEASKNREYG
                     RAFFESVNTNDPLFKGFNPLAPVWMSQGDNITQLPAEFEKIALTTDIPVAAFKARGEA
                     TWGVQFQPEALQTEEGSKILSNFLDLCNCARNWTPASFIESTVAELKRTLGNDKVVLG
                     LSGGVDSSVTALLLNKAIGKNLTCIFVDHGLLRKYEFETVLKDYESFGLNVIGVEAKE
                     KFYRELAGVSDPEQKRKIIGKGFIDVFDEEAKKLDNIKWLGQGTIYPDIIESLSITGT
                     TIKSHHNVGGLPEVMKLKLVEPLKLLFKDEVRRVGAALGMPKRFIDRHPFPGPGLAVR
                     ILGDITPEKVRILQDADHIFISLLHEWGLYDKVWQALTVLLPVRSVGIMGDERTYENA
                     VVLRAVTSTDAMTAEWSPLPYDFLAKVSNDIINKVKGVNRVTYDISSKPPATIEWE"
     gene            complement(13525..14574)
                     /locus_tag="PKY68_00480"
     CDS             complement(13525..14574)
                     /locus_tag="PKY68_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOH23549.1"
                     /translation="MTQQVVYGIPASITLAQGLLESGAGNSTLAKNSNNHFGIKCHND
                     WKGKTYSHFDDGEMSCFRKYSKVEESYKDHSIFLSERERYAFLFDLNIKNYKGWARGL
                     KKAGYATDRHYDKKLIKIIEQYDLNKIDKLALNKRRAKKLIRNWDKEYPEEIAKAEKP
                     SVQTESKTTAQKEKSPKVEKSSKKQEPAIGKKIQPAEKAKDYSYIAETSRERQIQRNE
                     RNERTDNLPHVINAVQNHTILYKGLTPYIIAQYGDNFDNISDEFGLSPQRLKKWNEWP
                     PRYKISVGDIVFLSKKAQSWDGEVGVYVVREGDSMHSISQQFGLRVSAIYQLNGMEFG
                     ESLEVGQELKLRAQE"
     gene            14734..15201
                     /locus_tag="PKY68_00485"
     CDS             14734..15201
                     /locus_tag="PKY68_00485"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOH23550.1"
                     /translation="MYKKEIKTNITFCSFDELSKQEKELIEAAKKASLDAYAPYSGFK
                     VGSALLLDNGEILVANNQENAAYPSGLCAERVVLFYANATYPKNRVLSLAVAANKEGI
                     FTENPISPCGSCRQVLLESEQRQNTAIRLLLCGEKEVAVAESVASLLPLSFNL"
     gene            complement(15204..16556)
                     /locus_tag="PKY68_00490"
     CDS             complement(15204..16556)
                     /locus_tag="PKY68_00490"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOH23551.1"
                     /translation="MPKHFIPGVLVTLLFTALSCTEGQYTGGSIQPPGDVVSTDTASF
                     FLTTETVKVDSILYKNSVALLGEFTDAFFGTTRAEFLAQVYCPFNFTFDASIRRIDSA
                     YLYLYYNDWFGDSLALMEMSVYELTKAIDHTKPYYTNINTDDYYDKSIKLGSISYSTG
                     EPFTDIKTSSDYSCIRVNIDTAFAGEILRKNRNHPEFFANPRVFADSVLHGIAVSPSF
                     GNGNLMYIKHAEFEFCYRYSYTDTMGVVKDTTGACYFPVTKEVRQINKYQHPDLAGYL
                     PLNSGDSLNYIFSPAGLFTRIELPIEEILSRLEGKNINSARIKAVVTHLDNSKWGMLP
                     PDNLLLLKEEDVHDFFAGYNNADKLYSFLADYNTRNDLFSFDLSSFLQKTVRNDSSSF
                     RPYNKLLMIPVSKIRLNNVSTSTLFLVQNILPNAATIRSATHPQKPMKLEIVYSNKVE
                     "
     gene            complement(16558..17370)
                     /locus_tag="PKY68_00495"
     CDS             complement(16558..17370)
                     /locus_tag="PKY68_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOH23552.1"
                     /translation="MKPAKVLFICQEIMPYVPETELSSICRRLPQFVQDNGNEIRTFM
                     PKYGCINERRNQLHEVIRLSGMNLIIDDNDHPLIIKVASIQAARMQVYFIDNDDFFQR
                     KALVCDESGVDFVDNDERSIFFVRGVIETVRKLRWTPDIVHCHGWFSALTPLYIKKAF
                     DDDPYFREAKVIYSIYDDAFDSNFRKLFNRKLLLEGIEQEDIEEIKNKADYVNLSKLA
                     IRYADGIIQASQKINSKVKKAAEESGKPFMIANESEDFSDYGEFYDLLLEQA"
     gene            17525..>17857
                     /locus_tag="PKY68_00500"
     CDS             17525..>17857
                     /locus_tag="PKY68_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOH23553.1"
                     /translation="MQVVKTKNQLRTILDAERSKGCSVGLVPTMGALHRGHISLVERC
                     VQENDFCVCSVFVNPTQFNDKDDLIKYPRRPEKDAEVLQKAGCSLLFMPDEQEMYPEE
                     DTRLFYFGP"
BASE COUNT         5022 a         3808 c         3991 g         5036 t
ORIGIN      
        1 ccccctgcaa aatgggaaac tcaattccca gtaaggtatt tattcgagtt ttaatcatat
       61 taaatcgtct tacatagtcc attccagcag acaagcaccc gtggtgaaac ctgctccgaa
      121 agcactgaaa accagttttt gtccggtttt taatttatta tttttattca attcgtcgag
      181 caacatggga atgctggccg acgaggtatt tccgtagcgt tcaatgttgt ggggaaagtt
      241 tttttcgctt tgccccaaat acctcattat tgtgtcgaga attctcatgt ttgcctggtg
      301 cagcaaataa tgatcgatct cgtccgcttt gacccctgcc agtttcaaga catctttgat
      361 atcgttcaga gaacagttta ccgccaattt aaaaacgtcc tgtcccttca ttaccaatgc
      421 accgtttcct tctgtttctt ccctttttat aaaaggtgta tcctgcaatt tgcggatttg
      481 atacaaggct tcggtattgc tgaaagtgga aagtttgaaa gatttaaaac cttccccttt
      541 accaaggact acagctccgg ctccatcccc gaaaaggacg caagtgtcgc gtttggtcca
      601 atccaccatg cgggtgggtt cttcggcgca aacaaccaga atatgttcca tgttgcccct
      661 ttcaaacaag gcgtcagcca tatccagtgc gtaaatgaaa ccggcgcagg ctccattaag
      721 gtcgatacaa gggcaatcgg ctcccagcat accctgaaga atacagctca gcgcaggagt
      781 cacgtattca ttgagcgtgt tggagcaaat gatataatct aaatcttctg ccttaatgcc
      841 tgcttcgtga attgctattg agcaagcttg cgctgccaaa tcttccagtc gctccgtcga
      901 aataacttgt ctggttttta ttcctgttct ggtttggatc cactcatcgc tggtatccaa
      961 aaatgtggcc agcatatcgt tgctgacggt tagggaggga gaagaactcc cggttcctat
     1021 aattttcatt tttaatttcc atgaatttga ggccgcaaat gtagctctga tatgggctcc
     1081 cggccaagtt tatgtggtga ataaaaatgg aaatgtggcg aaaacacgaa tatcggtggc
     1141 caaatcttgc tatttgtgac caaatagttt ttacatttgt ctctgtttgt tgattacatt
     1201 agctcaaaaa caggataaaa tgatcactca aaaagtaccc tcatacctgt tgcaaaagaa
     1261 ccaaatactt gggtctttgc tgtttacaat acttttttcc atcatttttt taaatattta
     1321 cactcctttt tcatctacag cctggtttga tttagggaaa tccgactttt ttttcttcac
     1381 cgtaggtgtt ttggctgcat cgacattgat tttaatcttg agcagaatct tggtattcaa
     1441 cagtaaaaag tggttcaaac ttactttctt aggttttttc gtttggttgt ttgccgagat
     1501 tgtactgata accacgcttt acacctttgt caccattacc tttatcgaac aaacggatgc
     1561 ttctttttgg actgtcttcc cccgggcttt gttatatact tcggtgattc tgattatacc
     1621 tgatacgatt tctgttttat attgtgccct gaacgataaa aacagaacct tgcgccttct
     1681 caattataaa gattttgttt ccgacgatga attggctaac aaagacaagg aattgatcca
     1741 tttgaccgat aattatggca atctgaaact gtccataaaa ctggataatc tttattatat
     1801 agaatcacac gacaattaca ttaaggtgta ctacatgaac aaaggtgcta tgtgcaatta
     1861 tttattgcgt tgtaagctaa aaactataga ggaaagcttt gctaacagct cattgatgcg
     1921 ttgtcaccga tcctatatta tcaattcgga caaggtaaag gtaatccgca aagaaaaaga
     1981 aggtatttac attgaccttg attcggaaaa ggttaaatct attcctgttt ccaaagggta
     2041 ttcacaaaag gtcatgcgct attttgggag cgattctgtt tagatttatc gccccgggct
     2101 ttaaatatcc ggtacaaaaa ataggccaga aacagactga caccaatctg tgaactcagc
     2161 atgattaaca aagcagtcgt attcattatt ggtttagttg ttctatatca aagtaattat
     2221 gagatttctt gttggcggct ttccaaacaa aaaaacaaag catcaaaaac aagattaaca
     2281 agccgattct ggcactgtca atataaaaga cggtatttac gattttaccc ttataaagca
     2341 catctcctgc cttgattctg tctccttccg aaaccattaa tgtgtgccgg cttggtaagt
     2401 gatagctctt aatggtgtta gcagacagta tttcaagcat atttcccttt tctgttgctg
     2461 taattttatg aaccaaaccg tcgcgttcgg cgaagtattc tttggcaaac cacgtgttgt
     2521 tggggccgat atctttatgc gacaattctc cgataatgct gtttttatcc agttcccagc
     2581 cctgcagact caacaaactc caatcgtcgt ttttgggttt tactgcggag gcaagtaaaa
     2641 taagtatcaa catggtggga gtgatgtacc tgagtataaa tttaaaaatc ccgggcatac
     2701 gcatgtcggc tccttggtga ataagttccc agccgcggtt aactccaaaa atccaggaaa
     2761 acaaaatgat ttcggcagtg gcgtaaagta ccagggcaac ggtacctccc caataatcgt
     2821 attcatcgaa tactcctttg gagaaaaaca gtaccgttgg aattccaaat gctaagataa
     2881 tgctgccgaa ggcaagtgct cctttctttc tcgaccagcc gaaattatct tttaaaaacc
     2941 cgacaattgg tgcccccata gccaatgagg aagtaattcc tgccccgaac agtaagccga
     3001 aaaaggctat ccccgccaaa ctggccagga catctcccca ttgttcaaac aaatagggca
     3061 tagtacggaa gcccagacca aaacctccga tctggcacaa atccatcact ttgtctatgc
     3121 caaaataacc gatggaaatg ggaatgatga tggatccgcc gatgacaatt tcgacgaatt
     3181 cgttcataaa accggctgtc atagagttga gtaccacatc atcattttct ttcaggtaag
     3241 aagcatagca ttgtatgcag cccataccca gcgatagggt aaaaaagatc tggccggcgg
     3301 ccgatagcca taccttggga tcggataaag agttgagatc gggcgtccat aaaaactcca
     3361 gacctaccca gccatcgtaa attgccccct gttcaccggc tttgatagtg actattttta
     3421 ctaccagaaa aagaccgaat attatcaaca ggggcatgaa aaatttagct gctctttcta
     3481 tgcctttgcg aagggtctgg ctcagtatcc aaacattgat gctcaggcaa aggatataaa
     3541 aaagaattga ttcgtagggg atgcctgtgg ttgaggtgct tattcttagg taatcgtcga
     3601 agaagtcgga gacctgtatt tcggtcatcc cgtcgaaggt gcgtattatg gagtggtaga
     3661 cgtaagagag tgtccagctt tcgatgtagc aatagtacga cgcaatcaca atgttggaaa
     3721 aaagcccgat gactccgatg tatttccaat agggtcgctt gtcgaggctt tgaagcacaa
     3781 aaggggaaga atgatgtccc cggaggcccg catacctgcc ggtactccat tctaccaaca
     3841 tcagagggat acccaagagt acgaatgaaa caagataggg aatgatgaaa gctcctcctc
     3901 cgttttgtat ggcctgaatc gggaaacgta gaaaattgcc cagcccaacg gcatttcctg
     3961 ccatggcaag gattagtcct aatcgggtgc cccaggcttc ttttgcttgt atcttactca
     4021 tagtgcttac aatttttgaa cagtcgtaaa ctaactttca aaaactcaga gctgattcat
     4081 tgagaattaa ttgcaataaa gcggcaaaag tagtgaattt ttccttacat ttgcgggatt
     4141 tttatagtga ttaaaatggg cgatgaaggc ccacagagac aatttaaaca agatttttat
     4201 gatgacaccg attgtcaaaa caattgaatt gggtgatgga aggaccatta ccatcgaaac
     4261 cggtaaatta gccaaacagg cagacggctc tgtagttgtc cgcatgaaca acattgtatt
     4321 gctggctact gtttgtgccg acaaagaagc agtccccgga accgatttta tgcctttaca
     4381 ggttgaatac agagaaaaat tcgcctcctc gggacgtttt cccggcggtt ttcttaaaag
     4441 agagggacgt ccctcagatt ctgaaatttt gacctcacgc ttagtcgacc gggctttacg
     4501 ccccttgttt cccgcagatt ttcacgccaa taccattgtt aacgtgatcc ttttttctgc
     4561 cgataacgac tgcaacggag atatgcccga tgctttggcc gggctggctg cctcggcagc
     4621 tttggctgtt tcggatattc ctttcaacgg tcccatttca gaagtccgca tagctcgtat
     4681 tgacggacaa tttaaattga atcccacttc ggctgaactc aaaaaagccg acatggatat
     4741 tatggtaggt gccaccctgg agaatattat gatggttgaa ggtgagatga gcgaggtgca
     4801 agaaagtgat ttgctcgagg cgctcaaaat tgcacacgaa gctatcaaag cccaatgccg
     4861 tgcccagctt gagttggctg aattggccgg taaaacagtt aaacgcgagt attgtcacga
     4921 agaaaacgac gaggagttgc gcaaggatat ttgggataaa acttatgcca aggcctacgc
     4981 agaagccact tcggccaatc ccgacaaaca tcagagagaa gccaacttca aggcgatata
     5041 cgaagaatat atagccgcaa tggacgaaga aacagctttg gaaaaagccg agatgatttc
     5101 ccgttattac catgaagtgg aaaaagaagc catgcgtcgc agtattcttg acgaaggcaa
     5161 acgcctggac ggcagaacga ccacagaaat tcgccccatt tggtcagaaa tagattgtct
     5221 acccggctgt catggttctg ccatttttac tcgtggggaa acccaatcgc tcacttcggt
     5281 tactctgggg accaaactgg acgaaaaaat tgtggacgat gctctgaacc aaagcaccga
     5341 aagatttctg ttacattata acttccctcc cttttcaaca ggcgaagccc gtgccagccg
     5401 cggggtggga cgtcgcgaga tcggtcacgg caatttggcc catcgtgcct tgaaaagaat
     5461 gttacccgaa gaatatccct atgtaatccg cgtggtttcc gatattttgg agtcgaacgg
     5521 ttcttcgtcc atggctacgg tatgtgccgg aaccctggcc ctgatggatg caggggtacc
     5581 tataaagaaa ccggtttccg gtattgccat ggggcttatt accgataaca aaaagttcgc
     5641 catcttgtcg gatattctgg gagacgaaga ccatttggga gacatggatt ttaaggtaac
     5701 aggaaccaag gacggcatta cggctactca aatggatatc aaagtggacg gactgcctta
     5761 cgaaatactg gaaaaagctt tgatgcaggc tcgcgaaggc cgtatgcata ttctcggtaa
     5821 gattactgaa actatgccgg ctccccgccc cgaactcaaa cccaatgcgc ctcgcattga
     5881 acttatgtgg atacccaaag aatttattgg tgccgttatt ggcccgggtg gaaagattat
     5941 tcagggaatg caggaagaaa ccggtgccat cattactatt gatgagattg acaaccaggg
     6001 tagggtagaa attgctgccg ttaacaaagc cagtatcgac gccgctataa tgaaaatcaa
     6061 aagcattgtg gctatgccgg aagccggaga agtttaccgg ggcaaagtcc gctccatcat
     6121 gccatatgga gcctttgttg aattcatgcc cggaaaagac ggattactgc atatttccga
     6181 gattacctgg aagcgcctgg aagcgattga agaagccggc ttgcaagaag gggacgagat
     6241 cgatgtgaag ttgctcgaca tagatcccaa aaccggaaaa ttcaaattat ctcacaaggc
     6301 attgctcgag aaacccgaag gatacgaaga acgccccgaa agggagcgtc gccccagacc
     6361 cgataagcgt cagggaggcg acgatgccgg tagaggcagg ggtggtcgtc gccgtccgga
     6421 ttcaaatcgc gacaggtaag acattaaatc cagaataatg aaaaaagatc agcgaaaatc
     6481 ggtggtgcta agggggtata ataccgccac tgaagccaac attgttaaaa gtatgctcga
     6541 atctaacggg atagcctgtt ttttgagcaa cgaaaccagt gtgtttacac ccggattttt
     6601 ttcggctagc tctacagtgt tattgcacgt aatggaagag gatattgaaa aggcctcatc
     6661 tttgatcgat cagattccgg aagcctttag tgaggaagaa agctaagcgg aggtattatt
     6721 aagatttaga gcgggctcct cccttgaaag gtttacggga cccgcttttt ttgttcctgc
     6781 cccgtgtcgt cgcttttttg ttttcggact tcactttgtt aacaaaccgg cttcgcctgg
     6841 ctttttgcgg ttgataggga ggcccttcgc ctaatttccc cgggagcgta ttttttacca
     6901 cttctttttc cagaaagcgc tcaatttgca ggaacttgta ttggtcctgt tcggaaacaa
     6961 aggtaacggc tctgcctttt tcgcctgcgc gggcggtacg tccgatgcgg tgaacgtagt
     7021 cttccgggtc gtgggggaca tcgtagttaa tcaccagggc aatatcatca atatctattc
     7081 cccgggagac aatgtcggtt gctaccagaa tgttgacttt gccggttttg aaactgagca
     7141 tcaccttttc tctctcggct tgctctaaat cggaatgcat ttcgataacg gaaagattca
     7201 gacgtttcag aatgcggcgg acttctttta ccttgatttt ggaagaggca aagatgagta
     7261 ctttttcgcc tgtctgcata gaactaaaca agatattaac caattcaggt ttttgtcgtt
     7321 catagcaaat gtaggccgat tgatcgatgt tttcgttggg tttggaaacg gccagcctta
     7381 tttctttggg ctgatgcatt atattttttg ccagctccct gatttttgga ggcatggttg
     7441 ccgaaaacaa catggtttgt cgtttggtag gcaattcttt gataataagc attatgtcgt
     7501 cgatgaatcc catatcgagc atgcggtcgg cttcgtcgag gataaagtat ttaacctgac
     7561 tcaggtcgat caatcccaat tttatatacg aaagcaaacg tcccggagta gcaataacga
     7621 tgtcgacgcc catatccaga ctttttttct gttgctccca caccatgccg tcgtttccac
     7681 cgtaaacggc aatggacgac accggtaaat aataagcaaa accttccact tgttgatcta
     7741 tttgctgggc caattctctg gtaggcacca taatcacagc tcggatggca tcgtgtgcat
     7801 gtttgtccaa cacaagatta tttagaatcg gcagaagata agcggctgtc ttaccggtac
     7861 ccgtttgagc acaaccgatt aaatcattgc cctcgagcaa aacaggaata gccaattcct
     7921 gcaccggcgt gcattcccga aaattcatag cccacaatcc gtcaaggagt tcttcttcta
     7981 aatcgagttc ttcgaatttc attcaaccaa agaattaaat aatctatgca aaggaacgaa
     8041 aaaaaacatt acttttggca caatataagc taatacccgt aaatactgtt cttatgaaaa
     8101 caatcaaatt tttaagtctg gcttggctgt taatcttgct tttcagcgca tgcgatcgag
     8161 gttatgaatg tgtacccgga gatcctatga ataccagaat atacaccttg gacaacggcc
     8221 ttaaagtgta tatgaccgta aataaagacg agccccgcat acaaacttat atcgcagtca
     8281 aagtgggagc aaagaacgat cctgccgaaa ccacaggttt ggcccattat tttgaacacc
     8341 tcatgtttaa aggcagcaag cagttcggga ccatgaacta cgaactggaa aaaccgatac
     8401 ttgaccggat tgaagccctg tttgaagagt acaggtctaa gactgatgag gcagaacgca
     8461 aagctattta cagaacaatc gacagccttt cttatgaagc ctccaggttt tttattccca
     8521 acgagtatga caaactcatg acagccattg gcgcttcggg aaccaatgcc tatacgggat
     8581 ttgatatgac tgtttatacc gaggatattc cttcaaatca aattgagaat tgggcaaaaa
     8641 tccaggcaga ccgttttcaa aacaatgtga ttcgtggttt tcatacagaa ctggaaaccg
     8701 tttatgaaga aaaaaacatg tccttgacca atgattcccg taaagtgtac gagaaggtat
     8761 tggccatact ttttccgcac catccctacg gtacccaaac agtgctcggt actcaggaac
     8821 acttgaaaaa cccctctatt accaacatca agaattatta cagcacttat tatgtgccta
     8881 acaacatggc catttgtctt tcgggagatt tcaatcccaa agaaatgatt caggtgataa
     8941 aaaaatattt tggagaaatg aagcctgccg tttccatacc tgaactggaa tatgaagagg
     9001 ggaaggcctt gagtgagccg gttgtttgtg aagttttggg taacgatgcc gaaaacgtca
     9061 ccttaggctg gagttgtgtg ggcattggcg atgaagagtt caatctgctt accctgaccg
     9121 atatgatttt aagcaacggc aaagccggtt taatcgacct ggaccttatc cagcaacaga
     9181 aagtgctgaa cgccttttcc ggagcttatg agatggccga ttacagcgct tatgtcatac
     9241 agggcaggcc caaagaaggg cagagcctcg aagaagtcaa agatttgtta ttggaagaaa
     9301 tggccaaatt gaaagcagga gccttcgatg attatttgct tgaggccagt atcaataatt
     9361 taaagttgag tagaatcaga gaaatgcaaa gcaacaacgg ccgcgccgat atgtatgtca
     9421 gagcctttat taatgagttg gattgggcgg atgaagttaa acggatggat gatctgagcc
     9481 agatcagcaa agaagacgtg gtggcttttg caaataaatg gttcaacgat ggctatgctt
     9541 tagtctacaa acggcaggga attgacccaa acgaaaaaaa gattgcaaaa cccgaaatta
     9601 ctcccattct gactaaccga gatactgtca gtgctttcct gaaagagatt cagcagacga
     9661 tagtaaaacc catacagccc gtctttttgg atttcaaaaa agatttttcg cgtttcatgg
     9721 tacaagccga ccttcctgtg ttatacatcc aaaatcaaac cgatgatctt tttgaattga
     9781 cttattggtt tgatatgggt accaatacca acaaattatt gagtctggct ttttcgtatt
     9841 tgaattattt gggtacttcc gaatttaccg cagaacaaat taaaagcgag ttctacaaaa
     9901 tggcctgttc ttataatttg tctgtcggtt ccgaaaaagc gcagattcga ctgacgggac
     9961 tgggagaaaa tatgcccgaa gcattgaatt tacttgaaaa attactgttt gatgctcagg
    10021 tcaatcaaca ggcatttgac aacatgatcc aggatgtcct aaaaagcagg gccaatgcta
    10081 agttgagcca aagccgtatt ttcaacatgt tgcgcaatta cgctgtttac ggacccggct
    10141 ccccggcgac gaatgtttta agtgatcagg agctgttaaa tcttaaaccc gaagaattaa
    10201 tcgaagtttt aaataaactg acctcctata gtcataaagt attgtattac ggtccggatt
    10261 caaaagaaca attgacaagt gtgttaaaag aaaaacacct ggtaccggag acattgtttg
    10321 aattgccggc ccccaaagaa ttcccctatt tattgacaga gaacaaccag gtcttatttg
    10381 cccaatacaa tgccaaacag ctctatttgt ctatgtattc caataatggg gaagtatttg
    10441 atctgcaaaa ggaacctttg accactttgt ataatgaata ttttggcggg ggcatgaact
    10501 ccatagtatt tcaggaaatg cgcgaagcca gaggtctggc ctacatggcc gccgctgctt
    10561 atgtgactcc gtccagagcc ggccgcccct attccatggt cagcatcatt gcaacgcaaa
    10621 ccgacaaaat ggacgatgct ttgcaagcct tcaatcaaat tatcgaagat atgcccgaat
    10681 cggaagccgc cttcaaactt gccaaagacg gtcttttttc cagactgcgc accgaaagga
    10741 ttttacgttc caacatactc tgggcttatc tggatgctct tgattacgga ttggaagaag
    10801 attctcgcaa atatttatat gaaagtctga ccgatctcaa tttggaagat ctgaaaaact
    10861 tccagcaaca atggattaaa aaccgctcct acagctattg cgtgctgggt gatgaaaaag
    10921 aggtggattt cgataattta gctcagtatg gaccggtaac taaattagag ttggaaacgt
    10981 tgttcgggta ttaaggcccg gacaacgttt cttttaaagc gtcatagcct cgctaaatcc
    11041 gacgaacatt gcacgtatta acccgaacag tgtttctttc aaagaaggct tcatttatta
    11101 atacacgaaa tcttatgtta aagtatttat gtgccaccat ttttaccatg ggtgtgattt
    11161 gctcatgcac cagaatcgaa ggcgatggag tgcttgttaa agcaatgcgt tccagtagtt
    11221 cttttagcga agtggattca aggggtgaat ttgaagtgta tttatattat gctgatagtt
    11281 gcaaagtgga agtagagtgt gaatcgaata ttctgcaata catagtaaca aaatttgtta
    11341 gaaataagct ggttgtagat aaaaagaaca atgtgttgat agccaacaat atgccaatta
    11401 aagtttacgc atccactccc tctcttaaat ttgcggaact tagtggatca ggtagaatgg
    11461 tcgttgatga attacattcc ggcaatctta ccttatcctt gtcaggttcc ggagaaatca
    11521 tttacaaaga ctctgttgaa tgtgagagcc ttacaatgga catttccggt tccggtaaaa
    11581 tcaatgcacc gtacgtaaaa tccggacaaa ccaatattga tatcgatggt agcggagttt
    11641 gcaccgtcga tagcatttcc tcggaaaaac tggacattga tatagacggc tcgggtggca
    11701 tcaacctgag cggacgatgt gattatgtgg atcttagaat agcgggaacc ggaaaacttg
    11761 aagccagcga tttgcttacc aaaaggtacg acatccgcgt caacggctca ggttgcgctt
    11821 acattaatgt gagcaacgaa ctgaacatta ttgtcaacgg cagcggtaat atttattacc
    11881 tgggatatcc taaaattacg atgacgggca gcggtaacgg cggtctgatc aatatgaacc
    11941 cctgatgttt attcccactc aatggtagca gggggtttcg aactgatgtc gtaggtaacg
    12001 cgattcacgc cctttacctt gtttatgatg tcgttagata ctttagccaa aaagtcataa
    12061 ggcaatggag accattctgc ggtcatggca tcggtcgaag taacagcccg caaaaccact
    12121 gcattttcgt aggtgcgttc gtcgcccatg atacccacgg agcggacagg aagcaagact
    12181 gtcaatgcct gccagacttt atcgtagaga ccccactcgt gcagaaggga tataaaaata
    12241 tgatcggcgt cttgcaggat acgcactttt tcgggtgtaa tgtcacctaa aatacggaca
    12301 gctaatccgg gccccgggaa aggatgtctg tcgataaatc ttttgggcat acccaaagcg
    12361 gcgccaacac ggcgaacttc gtccttgaac agaagtttca atggttcaac cagcttaagt
    12421 ttcattactt ccggcaagcc gcctacgtta tgatggcttt ttatagttgt tcctgtaatg
    12481 gacaatgatt cgataatatc cggataaatg gtgccttgtc ccaaccattt aatgttgtcc
    12541 agctttttgg cttcttcgtc gaaaacatca ataaatcctt tgcctatgat tttccttttt
    12601 tgttcgggat cgctgacgcc tgccagttcg cgatagaatt tttctttggc ttctacacca
    12661 atcacgttca atccgaaaga ttcataatct ttcaataccg tttcaaattc gtatttgcgt
    12721 aaaagcccgt gatctacaaa gatgcaggta agatttttgc cgatggcttt gttgagcagc
    12781 aaggcagtga cagaggaatc caccccgccg gacaagccta agaccacttt atcgtttccc
    12841 agggttcgtt tcaattctgc caccgtcgat tcgataaacg atgccggtgt ccagtttcgg
    12901 gcacagttac aaagatccaa aaagttgctc agaattttac tgccttcttc ggtttggagc
    12961 gcctcgggtt gaaattgaac gccccaggtg gcttctcccc gggctttgaa cgcagcaacc
    13021 ggaatatccg tggtcagagc gattttttcg aattcagcgg gcagttgggt gatattatcc
    13081 ccttgcgaca tccaaacagg agccagggga ttaaagccct tgaacagggg atcgttggtg
    13141 ttgaccgatt caaaaaaagc ccttccatat tctctgtttt tggaggcctc tattttcccg
    13201 ccggctttgt ggacaatcaa ttgggctccg taacaaatac ccagcaaagg gaatttcttt
    13261 cttaccaatt ttaaatcaaa gtcgaaggcg tttttgtcgt aaacagaaaa ggggctgccc
    13321 gataggatga cccctttaat accgtcggta ttttcgggga atttgttgta aggatagatt
    13381 tcacaaaagg ctttaagttc tcttatgcgc ctggcaataa gttgagtggt cttagacccg
    13441 aaatcgagta cgacaatttt ttcttgcata atgggggaat tgacactatt tggggtgcaa
    13501 aggtataaaa agctttgcta ttttttattc ctgagccctc aattttaatt cctgaccaac
    13561 ctcgagactt tcgccaaatt ccattccgtt caattggtaa atcgcgctta cccgaagtcc
    13621 gaactgttgt gaaatactgt gcatgctgtc tccttcgcga accacataga caccgacttc
    13681 gccgtcccat gactgggctt ttttactcag gaagacaatg tctcctaccg aaattttata
    13741 ccttggaggc cattcgttcc atttttttag gcgctgaggg cttaagccga attcatcgga
    13801 gatattgtcg aagttatccc cgtattgtgc aatgatgtac ggtgtcagac ccttatataa
    13861 aatcgtgtgg ttttgtacgg cgttaataac gtggggcagg ttgtcggtgc gttcgttacg
    13921 ttcgttgcgc tgaatttgtc tttctctgct ggtttcggca atgtagctat aatctttggc
    13981 tttttcggca ggctggattt ttttgccgat tgcgggttct tgttttttac tgcttttttc
    14041 aacctttggt gatttctctt tttgagctgt ggtttttgac tccgtttgga cggacggttt
    14101 ttcggccttc gcaatttctt cgggatattc cttatcccag ttccggataa gcttttttgc
    14161 ccttcgtttg ttgagtgcca gtttatctat cttgttcaaa tcgtattgtt caatgatttt
    14221 tatcagtttc ttatcgtaat ggcgatctgt ggcataaccg gctttcttta aacccctggc
    14281 ccagcctttg tagtttttta tgttcaaatc aaacaaaaag gcgtaacgct ctctctcgct
    14341 cagaaatatg gagtgatctt tataagattc ttctaccttg gaatattttc taaagcagct
    14401 catttctccg tcatcaaaat gagaataggt ttttcctttc cagtcgttgt ggcattttat
    14461 cccaaagtga ttattgctgt tttttgccag ggtgctgttg cctgcacccg actcaagcag
    14521 gccctgcgca agagttatgc tggcaggtat gccgtatacg acttgttgtg tcatggccag
    14581 cggggtgtat tttcggatat agtcttcata aggcttcaag cgttcctgtg cctgtagagg
    14641 gagacaaaaa aagcttaaaa caaataaagt taaaaatgta aatctgctca tagaaatggt
    14701 atttttgtgc tgaagacgta aaaatcagtc cagatgtaca aaaaagaaat caagacaaat
    14761 ataacatttt gttcctttga tgaattgagc aagcaggaaa aagaattgat tgaagctgcc
    14821 aaaaaggcca gtttagatgc ttacgcgccc tattcgggat ttaaagtggg ctcggcattg
    14881 ttgcttgaca atggggaaat attggtggct aataatcagg agaatgccgc atatccttcg
    14941 ggactttgcg ctgaacgtgt tgtgctgttt tatgctaatg cgacatatcc caaaaacaga
    15001 gttcttagtt tggccgtggc ggccaacaaa gaaggaattt tcactgaaaa tccaatcagt
    15061 ccctgcggaa gttgcaggca ggttttactc gaaagcgagc agcgacaaaa cacagcaatc
    15121 aggctgctgc tctgcggaga aaaagaagtg gctgtggcag aatcggttgc aagtttattg
    15181 cccttatcct tcaatctata atttcactca actttgttgc tgtaaacaat ctcaagtttc
    15241 atgggttttt gaggatgtgt tgcgctgcga atcgtggctg cgttaggcaa aatgttttga
    15301 accaaaaaaa gcgtactcgt cgagacatta ttcaatctaa tcttgctcac gggaatcatc
    15361 agcaacttgt tatagggtct aaagctgctg ctatcgttgc gcacggtttt ttgcaggaaa
    15421 gaacttaaat caaaagaaaa aagatcgttt cgggtattgt agtccgctaa aaaggagtaa
    15481 agcttatcgg cgttgttgta gccggcaaaa aaatcgtgga cgtcttcttc tttcaggagc
    15541 aagaggttat cgggtgggag catcccccat ttgctgttgt caagatgggt gaccaccgct
    15601 tttattctgg cactgttgat gtttttccct tccagcctgg aaagaatttc ttcaatgggc
    15661 aattcaatac gtgtaaacaa gcctgcgggt gaaaaaatat aattcagaga gtcgccgctg
    15721 ttcaagggca ggtagccggc cagatcgggg tgctgatact tgtttatttg ccttacctct
    15781 ttggttaccg ggaagtagca cgctccggta gtgtctttca ccacacccat agtatcggtg
    15841 tagctgtatc gatagcagaa ttcaaattcg gcgtgtttga tgtacatcaa gttcccgttc
    15901 ccgaaactgg ggctgacagc aatgccgtga agcactgaat cggcaaaaac tctcggattg
    15961 gcaaaaaact ccgggtgatt cctgtttttc cggagtattt ccccggcaaa ggccgtatct
    16021 atattaaccc ggatgcagga ataatcgctc gatgttttaa tatcggtaaa gggttctcct
    16081 gtagaataac tgattgaacc taactttatc gatttatcgt aataatcgtc cgtattgatg
    16141 ttggtgtaat aaggcttcgt atggtctatg gcttttgtaa gttcatatac gctcatctcc
    16201 atcaaggcta gagaatcgcc aaaccaatcg ttgtaatata aatacaaata agcagaatcg
    16261 attcgccgga tgcttgcatc gaaggtaaaa ttaaagggac aatatacctg agccaaaaat
    16321 tccgctcggg tagttccgaa aaaagcatcg gtgaactcac ccaaaagagc gacactgttt
    16381 ttgtagagaa tggaatcgac ttttacggtt tccgtggtta aaaagaaact ggccgtatcg
    16441 gtcgatacaa catcgccggg cggttgtatg ctgcctccgg tatactgacc ttccgtgcac
    16501 gataacgctg taaacaatag cgtgaccaac acgccgggga tgaaatgctt gggcatatta
    16561 ggcttgttct aaaagaagat cgtaaaattc gccgtaatca gagaagtcct cactttcgtt
    16621 ggcaatcata aagggctttc ccgactcttc agctgctttc ttaactttgc tgttgatttt
    16681 ttggcttgcc tgaataatgc cgtcagcgta gcgaatagcc aattttgaca gattaacgta
    16741 atcagcctta tttttgattt cttcgatgtc ttcctgttct atgccctcca gcaacaactt
    16801 gcgattgaaa agcttccgga agttgctatc gaaagcatcg tcatagatag aataaattac
    16861 tttggcctcc ctgaaatagg ggtcatcgtc gaaagctttt ttgatgtaca ggggagtcag
    16921 ggctgaaaac cacccgtggc aatgcacaat gtcgggagtc cagcgcagtt ttctaacagt
    16981 ttcgatgact ccacgtacaa aaaatatcga cctctcgtcg ttatcaacga aatcaacacc
    17041 cgattcatca caaaccaggg ctttgcgttg gaaaaaatcg tcgttatcga taaaataaac
    17101 ctgcatacga gccgcttgga tggaagcgac cttgataatc agcggatgat cgttgtcgtc
    17161 aataatcagg ttcatgcccg acaacctgat tacttcgtgt aattggtttc tgcgttcgtt
    17221 tatacagccg tatttaggca taaaagtcct gatttcatta ccattatcct gaacaaattg
    17281 aggtaaacgg cggcagatgg acgaaagttc cgtttcggga acataaggca taatttcttg
    17341 acagatgaac agtactttcg ccggtttcat aaaaacataa agattgtaaa catatggctg
    17401 caaagatact aaaaaaaata ggtggatgtg caagaaatga gcgttttatt tttttaaatg
    17461 gcaaaaaatt ggttcttttg cgccctcctt gtaaaggaga attcgagcaa acttccagac
    17521 gtttatgcaa gtagtaaaaa caaaaaatca actaagaacg attttagatg cagaacgcag
    17581 caagggctgt agtgtagggc ttgttcccac aatgggagcc ttacatcggg ggcatatttc
    17641 tttggtagaa cgttgtgttc aggagaatga tttttgtgtt tgcagtgttt ttgtaaatcc
    17701 cacgcagttt aacgataaag atgatttaat caaatacccc aggcgaccgg aaaaagacgc
    17761 cgaagtgttg caaaaagccg gttgcagcct gttattcatg cccgatgaac aggaaatgta
    17821 ccccgaagaa gatactcgtc ttttttattt cggtccc
//
LOCUS       DAOSGL010000007        17142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_2710_length_17142_cov_4.842629, whole genome shotgun sequence.
ACCESSION   DAOSGL010000007 DAOSGL010000000
VERSION     DAOSGL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17142
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_17142_cov_4.842629"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKY68_00505"
     CDS             complement(<1..272)
                     /locus_tag="PKY68_00505"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOH23554.1"
                     /translation="MKKFSYRCIVLLIFLVAGFAGAAHAQLVRGIVSEADGEPIIGAT
                     VVLTDNTSVGTITGLDGSFSLELPDAQNAVLSVSYVGFQTKEVPVN"
     gene            786..1187
                     /locus_tag="PKY68_00510"
     CDS             786..1187
                     /locus_tag="PKY68_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23555.1"
                     /translation="MPWKRIAFCSILTILILYSFRNFIIVHSVKPLRIELEVHADKNV
                     WLECFYTTQAEENFAAGKSVRYLFQPKDSGLISISLPVEHLHKIRLDLGEMPGKIRLG
                     TLRIPGHTTIGYEAGIRYYNIDNVLINKGGG"
     gene            1283..2458
                     /locus_tag="PKY68_00515"
     CDS             1283..2458
                     /locus_tag="PKY68_00515"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HOH23556.1"
                     /translation="MVALIIGFISFTSLYTLFSFLEKLQLSFTDLVLVVAFFALLPLP
                     AAKINKDKISVEENRTLAKFPALIVGKQVNNNFGTDFEAWFNDRFFMRKTLVWLHNSI
                     KKEVNIHNQNKYILVGKDGWAFCKDDGDIQNYQNLKLFRPEELKHIAQYLSSIDRWCN
                     ANNIKFYFLIAPDKNKIYGEYYKFARKIRPDSESRTQQLIRYLNENTRVKVAYPYESL
                     HAAKQNGFLLYFKNDIHWTHLGAYCGYTDLMELIVKDFEISPVRFEKTTTYKYPRGDA
                     NILFPEGVKEDTITLYHCPVLPINYTLTGEMETKKNLFYHNPKGRYSAVFFRDSFCTL
                     LADYYLGNTFEHLSLKWQYEITPKDLDFVVGKQADIVVLEQVENFIPKLLSVEILKD"
     gene            2463..3866
                     /locus_tag="PKY68_00520"
     CDS             2463..3866
                     /locus_tag="PKY68_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOH23557.1"
                     /translation="MLFSSMSFIYLFLPVVCLLYFLVRANLRNYVLLGASIFFYAWGE
                     PEYLAIMLLTILVNYVGGLLIDKYPKYKKLSLALTITVDLGFLAYFKYFNFFIENINL
                     LLKGGGLNFIEVIMPIGISFYTFQAISYAVDVYRGDVKVQQDIGKLALFICLFPQLIA
                     GPILKYNDIAHQIDSREVNPEKVTMGVKRFIIGLSKKVLLANTLGAVADKIFDLSPDL
                     VQTSIAWVGSLAYSFQLFFDFSGYSDMAVGLGLIFGFHIKDNFNYPYISKSITEFWRR
                     WHISLSTWFKEYLYIPLGGNRVSKPRNYFNLAVVFLLTGFWHGAAWNFVVWGIWHGLF
                     IIFEKVTGWHKENSRQWINPVKHLYTIFVFIIGWVMFRAENLSYAWQYIKNMFGLAGN
                     KTILYEAAYYFDNIEIIAFIAALLCSVPLFSNMLNIPQERKWLRWLVNAWLLFLFILS
                     TSAIAASTYNPFIYFRF"
     gene            3998..4471
                     /gene="argR"
                     /locus_tag="PKY68_00525"
     CDS             3998..4471
                     /gene="argR"
                     /locus_tag="PKY68_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOH23558.1"
                     /translation="MKNKANRMKSIRKIVHETRISSQEELLKLLTEEGFSLTQATLSR
                     DLKQLKIAKMPDYEGGYIYVLPEESSPLSRTVRMKSQDVHFIQSGFESLAFCGHFAVI
                     KTKPAYAGGIAAEIDKKGSKLILGTIAGDDTILVIPKEKTDRQSLMQALSEIIPL"
     gene            4554..5111
                     /locus_tag="PKY68_00530"
     CDS             4554..5111
                     /locus_tag="PKY68_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH23559.1"
                     /translation="MDKKFEIVVANASHLQYVDTILNTIEEAAKIRGTGIAKRSPEYL
                     TQKILEGKAILALVGDVFAGFCYIETWGNKQFVANSGLIVVEQYRQLGLAKKIKKKAF
                     KLSRTKFPDAKLFGLTTSAAVMKINYELGYRPVTFAELTDDEAFWKGCQSCVNYPILA
                     EKNRRFCMCTAMLYDPKEHKRHRRI"
     gene            5185..6387
                     /locus_tag="PKY68_00535"
     CDS             5185..6387
                     /locus_tag="PKY68_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOH23560.1"
                     /translation="MKEKVVLAFSGGLDTSFCVKYLSIEKNFEVYTAVANTGGFSQED
                     LKVIEDKALRLGAVKHVSLDITQEYYEKSIKYMVFGNVLRNGTYPISVSSERIFQAMA
                     IIEYAKSVGAKYVAHGSTGAGNDQVRFDLTFQILAPEIKIITPTRDMMLTREYEIDYL
                     KKHGYRADFTKMEYSINKGLWGTSIGGKETLHSEQTLPESAYPSQITKEGSEVLCIDF
                     HKGEISGVNGTAYTDKVKAIQKVEEIASAYGIGRDMHIGDTIIGIKGRVGFEAAAPIL
                     IIQAHKMLEKHTLTKWQQYWKDQIGTWYGMFLHEAQYLEPVMRDMEAFLSSSQGNVCG
                     RVSIRLRPYSFSLIGVDSPYDLMKTDFGEYGEVNKAWDANDMKGFTKILGNQMKIYYN
                     VQDRVNKK"
     gene            6384..7346
                     /gene="argC"
                     /locus_tag="PKY68_00540"
     CDS             6384..7346
                     /gene="argC"
                     /locus_tag="PKY68_00540"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOH23561.1"
                     /translation="MIRVGIIGGAGYTAGELLRVLINHPGVELVFAHSNSNAGKPVTS
                     AHGGLYGECDLVFTGELTFDNIDVLFMCSAHGDSKKFLESHSLPPKLKIIDLSTDYRD
                     ESDGFVYGLPELQKEKIRRADKVANPGCFATCIELALLPLAAAGLLTSDIHVNAITGS
                     TGAGVKPSSTSHFSWRNNNISVYKAFEHQHLKEIRQSLSALQKGFKAGIHFIPLRGNF
                     SRGILATAYTTCPWSLEEAIKAYTKFYREARFCFVCEENPDLKQVVNTNKCLLYLEKH
                     GDQLLIISVVDNLLKGASGQAVQNMNLMFGLDERAGLNLKSTGF"
     gene            7466..8596
                     /locus_tag="PKY68_00545"
     CDS             7466..8596
                     /locus_tag="PKY68_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH23562.1"
                     /translation="MNLFDVYPLYDLEIVSGKNCCIYDKQGREYLDLYGGHAVISIGH
                     SHPHYVRSICEQVARICFYSNSVVNSLQTKFATRLGKISGYEEYSLFLVNSGAEANEN
                     ALKLASFHNGRRKVVAFRKAFHGRTSAAVRVTDNPGIVAPVNQGMEVEFLALNDIPAM
                     QNCLSRGDVCAVIIEGIMGVGGVHIPQPAFLQALRKTCNETKTVLILDEIQSGYGRSG
                     RFFAHQYAGIRPDMITVAKGIANGFPMSGLIIGPEFKAVHGMLGTTFGGNHLACAAAL
                     AVLDVMEQENLIGNAARVGDFLMKKCKTIPQIAEVRGLGLMIGLEFDKPIKELRKELL
                     FEHAVFTGVSGSNVIRLLPPLSLSMEQAGVFIEKLVLALKTF"
     gene            8717..9505
                     /locus_tag="PKY68_00550"
     CDS             8717..9505
                     /locus_tag="PKY68_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HOH23563.1"
                     /translation="MKIGIIGVGNIGKAIVSGLAKSAFVPEENIVISDIEKSNLELIK
                     SSFPKIQSSTENKTAAQADLVIVAVKPWLVGGVLTEIAPLLETSRPILVIVAAGVSFE
                     QIYDKLPENLSNLPCYRFIPNTAIALRESMSLLSAANTTPEQDKLMMGMLKKLGKAVL
                     IHPDMMAAGTSLTSCGTGFALQFIKANVQVAIEMGFTPEQATELIAQTVKGAAELLLN
                     NKTTPEVEIRKVTTPGGITIRGLNEMEASGFTASVIKGIKASLL"
     gene            9510..10583
                     /gene="proB"
                     /locus_tag="PKY68_00555"
     CDS             9510..10583
                     /gene="proB"
                     /locus_tag="PKY68_00555"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOH23564.1"
                     /translation="MYIYRRVVVKIGSNVLTRKDGTLDVTRMSALVDQMAELHKAGLE
                     VILVSSGAVAAGRSEIQASRKLGQVEQRQLYSAIGQAILINRYYDLFRDHNIVCGQIL
                     TTKENFADRRHYLNQKNCMEVMLENKVIPIVNENDTVSVTELMFTDNDELSGLIAGMM
                     NAEVLLILSNVDGIFKGHPDDPEAELIEEILPKQELRQYISADRSSTGRGGMRTKYNI
                     ASKLAKEGIRVIIANGKKDNIIPDLFSPKPRNKHSLFLPAEKTSAIKKWIAHSEGFAK
                     GEVHINQGCTEALTQAQASLLPVGIERIEGEFEKGDIVRVIDHEGRQIGVGMIRCDSD
                     AARNIIGSKGQKALIHYDYLYLE"
     gene            10583..11833
                     /locus_tag="PKY68_00560"
     CDS             10583..11833
                     /locus_tag="PKY68_00560"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOH23565.1"
                     /translation="MSLENKFKAVKQASALLNDLSDIAVRQLLTDLAAEAEAQIPYLL
                     TENQKDLDRMNPADPKYDRLKLSAERIHGIASDMRQVAALSSPLGEILSKELRPNGML
                     IQKIRVPFGVIGIIYEARPNVSFDVFSLCIKTGNACILKGGSDAEYSNTAIVQLIRQQ
                     LLKHGFPDDAVLLLPPDRRAADELMKATAYVDLIIPRGSQSLIDHVRSHSLVPVIETG
                     AGICHTYIDSSADIQKAATIVHNAKTRRVSVCNALDCMLLHRSRLQDLPEICLKLQDE
                     QVVIYADPEALAALKSHYPGHLLQPAGEKHYGTEFLDYKMAVKTVKSTEEAIEHIARY
                     SSGHSEAIVCEDTEQAQLFCKKVDAACVYVNVSTAFTDGAQFGLGAEIGISTQKLHAR
                     GPMALKEITTYKYLITGNGQIRSK"
     gene            11833..12789
                     /locus_tag="PKY68_00565"
     CDS             11833..12789
                     /locus_tag="PKY68_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HOH23566.1"
                     /translation="MRHFTSITDLGDLKAAVSEARQIKARPFAWQNLGKNKTLLMIFF
                     NSSLRTRLSTQKAALNLGMNVIVLDVNQGAWKLETERGVVMDGDKSEHLLEAIPVMGS
                     YCDIIGVRSFARFESKADDYSEKILNQFIQYSGKPVFSMEAAIRHPLQSFADLITIEE
                     FKERARPKVVLTWAPHPKALPQAVPNSFAETMNLCDYEFVITHPRGYELSPEFSGKAA
                     IEYDQNKALEGADFVYAKNWAAFSDPNYGKILSRDRSWTIDSKKMALTNNARFMHCLP
                     VRRNMIVSDEVIEGPRSVVIPEAANREFSAQTVLKRILEGLD"
     gene            12930..13919
                     /gene="trpS"
                     /locus_tag="PKY68_00570"
     CDS             12930..13919
                     /gene="trpS"
                     /locus_tag="PKY68_00570"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOH23567.1"
                     /translation="MDTVLSGIRSTGHLHLGNYFGALRNFVRMQHENRCFFFIADYHS
                     LTTHPDPGSLHANVKDILCEYLAVGIDPEVATIYVQSDVPETVELYLLLNMHAYMGEL
                     AKTTSFKEKARKQPENVNAGLLTYPTLMAADILQHNADKVPVGKDQEQHLELTRRFAR
                     RFNNLYKVNFFTEPQPYNFDQELVKIPGLDGSRKMGKSEGNCIFLCEDPKLIKKKVMK
                     ALTDAGPTEPHSAMPESIENLFVLLKSVSSPDTVAYFEEQWNNCSIRYGDLKKQLAAD
                     IIAFTTPIRERYLDICNNDEYLHKVAVNGAEKARASAAATLREVRHIMGIRPF"
     gene            13968..14873
                     /locus_tag="PKY68_00575"
     CDS             13968..14873
                     /locus_tag="PKY68_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH23568.1"
                     /translation="MAIIACLLWSTAFVGVKIGLNYMSPLLFAGLRFSIAGILILLYY
                     GKFRAYFQAIKTHFWYILLIAFLQTVLMYGLFYTAMNMVPGSMGAMINGTGPLFAAIV
                     AHLFIKDDKITWRSGGSIMLGIVGIVIINIGRQTGGNAGGLEIIGVLLLIANNLVSGL
                     YNVIVKKSRRNISMLTLSSASLLIGGIVLILISIPFETAPANLNFPLEFWLALGWLSF
                     LSAAAFALWFTLLKRPGVKVSYLNSWKFIIPVFGAILSWLILPGEYPDIYSISGVIII
                     GASLLWMNNEALKDMFGKWLAKIKP"
     gene            complement(14906..15205)
                     /locus_tag="PKY68_00580"
     CDS             complement(14906..15205)
                     /locus_tag="PKY68_00580"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOH23569.1"
                     /translation="MPRGDKTGPKGQGPMTGRGLGFCKGHDSPGFTDNFEDGKDLGRG
                     RGLGGGLGRGRGLGFGGGMGRGRGLGFGGGMGRGRGRGFGRGRGMEPGAEASDGQ"
     gene            complement(15340..16368)
                     /locus_tag="PKY68_00585"
     CDS             complement(15340..16368)
                     /locus_tag="PKY68_00585"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOH23570.1"
                     /translation="MRELLFETLMRALMITGFVMLMMVVVEYINVQNKNRWAEKLRRS
                     PLTQILMATILGIIPGCMGAFAVVSLYTHRMIGLAAVVAAMIATSGDEAFVMFALFPG
                     KALLLHGILFIIALLTGWIIYLFCKKEREPAGPQYGFEVHQEDSCKCFDRSIILPQLK
                     KITFERSLLLLATLVFLLLLALGVIGHEGWGWEKITLLAGSLFLLFVFLTVPEHFLKE
                     HLYNHIIKKHLLRILLWSWGAFIVLYFVQTEQLLGTLVENNPYLILLAAVLIGIIPES
                     GPHLVFVTLFSQQLIPFSILLASSIVQDGHGMLPVLAESRKDFFLIKGISVLIGLVIG
                     TILLFLGL"
     gene            16559..>17142
                     /locus_tag="PKY68_00590"
     CDS             16559..>17142
                     /locus_tag="PKY68_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH23571.1"
                     /translation="MKYLIIGGVAGGATTAARLRRLDEKSEIIVFERGEYISYANCGL
                     PYYIGGIIKDRDQLFVQTPESFGQRFNLEVRNLSEVLSIDRRKKEVLVKDIRNGSEYR
                     ESYDKLILSPGAEPIKPMLPGINQEGIFTLRNVPDTDKIKQYIEQKQARHALVVGAGF
                     IGLEMAENLHHAGLKVTIVEMAEQVMTPLDYSMAA"
BASE COUNT         4892 a         4032 c         3754 g         4464 t
ORIGIN      
        1 ttgttaaccg gaacttcttt ggtttggaaa ccaacgtaag aaacacttaa cacagcattt
       61 tgtgcatcgg gtaattcgag cgaaaaagaa ccgtcaagcc ctgttattgt cccaaccgaa
      121 gtattgtctg tcagcaccac ggtagccccg attataggct caccgtctgc ctccgataca
      181 attcctctaa ccaactgagc atgagcggct ccggcaaaac ctgccactag aaaaatcaga
      241 agcacaatac atctgtaact gaattttttc ataaggatta ggattaataa attgattaat
      301 aataaacagt ctcttttttg taaccccaag ggggttttta acgtcactaa cagttcactt
      361 tgccggcatc tctttaaaat attccactaa gtttttaata gagttatcaa tcagacatag
      421 agaaatagag gcggcttttt cctcatttcc tcaatctttc acgataccag gttattaaca
      481 tatttaagtt aattactgca attatacaac aaactcaata ctcaaatcct tcccaaaatt
      541 aatactcttt ctgcgatttt gctgattatt tttctactta tgcattttta acttagcagt
      601 atcaatttta agctcgatat ttgtaaataa tcacttgcat tcacaaatca tagcctataa
      661 ttcttattta atttaagcat aatcatgaaa aaaacggccg gtcggtagaa ttgccggaat
      721 agtttttttt ttattttcaa agattttatg atctttgtcg ctcaaaaaaa taacaccttg
      781 cgcacatgcc ctggaaaaga atcgcatttt gctccattct gactattctt attctttata
      841 gcttccggaa tttcattatc gttcattcgg ttaaacctct ccgaatagaa ctggaagtac
      901 atgccgataa aaacgtttgg ttggaatgct tttataccac tcaggccgaa gaaaactttg
      961 ctgccggaaa atccgtacga tacttgtttc agcccaaaga ctcagggctt atcagcattt
     1021 ctttaccggt agaacattta cataagattc ggttggatct aggggaaatg cccggcaaaa
     1081 tccggctggg tactcttaga attccggggc ataccaccat aggatacgag gctggaataa
     1141 gatattataa cattgataat gtgctgatta acaagggggg ggggtagaca tatattcaca
     1201 acaggaagac ccctacatta tttttaatga acccctgaat attagcgcaa aacaaagcct
     1261 gagcctaaat aaaagcggcc tgttggttgc cctgatcatt ggttttattt ctttcacttc
     1321 gctttataca ttattttcct ttctggaaaa actccagctc agtttcacgg atctggttct
     1381 ggtagttgcg ttttttgcac ttttgcccct tcctgccgcc aaaatcaaca aagacaaaat
     1441 atctgtcgaa gaaaacagaa ccctggcaaa atttcccgca ttaatcgtgg gaaagcaagt
     1501 aaacaacaat ttcggcaccg acttcgaagc ctggttcaac gatcgcttct ttatgagaaa
     1561 aactctggtc tggctacaca attccatcaa gaaagaagtg aatattcaca atcaaaacaa
     1621 atacattctc gtggggaaag acggctgggc tttctgcaaa gacgatggag atatccagaa
     1681 ttatcagaat cttaaactct tcaggccgga agaattaaag catatcgcac agtatttgtc
     1741 gtcgatagat cgttggtgca atgccaataa tataaagttt tacttcctga ttgcgcccga
     1801 taaaaacaag atttacggag agtattacaa atttgcgcga aaaatccgcc ccgattccga
     1861 gagtcgtact cagcaactga ttcgttacct gaacgaaaac acccgggtca aggtggccta
     1921 tccctacgaa agcctccatg cagcgaaaca aaacggcttt ctgttgtatt ttaaaaacga
     1981 cattcattgg acccacctgg gagcctattg cggttatacc gatttgatgg aactgattgt
     2041 caaggatttc gaaatcagcc ctgtaagatt cgaaaaaacc accacctaca aatatccccg
     2101 gggagatgcg aatattttat ttcccgaagg ggttaaagaa gataccatca cactttatca
     2161 ttgtccggtt ttgcccatta attatactct cacgggtgag atggaaacca aaaagaacct
     2221 gttttatcac aatcccaagg gtcgctattc tgccgtattt ttccgtgatt ccttttgcac
     2281 cctgctggcg gattattatt taggcaacac ctttgaacat ctcagcttga aatggcaata
     2341 cgaaatcacc ccaaaagacc tggattttgt tgtcggtaaa caggcagata ttgtagtgct
     2401 ggaacaagtg gaaaacttta ttcccaaatt attgagtgtc gaaatcttaa aagactgatg
     2461 ctatgctctt ctcttcgatg agttttattt acttgttttt gcctgttgtc tgcctgctgt
     2521 atttcctggt cagagccaat ttgcgcaatt atgttttgct cggagccagt atcttttttt
     2581 atgcctgggg agaaccggaa tatttagcca ttatgttgct gactattttg gttaattacg
     2641 ttggaggcct tttaattgac aaatacccga agtataaaaa gctaagcctt gcgcttacga
     2701 taaccgttga tctgggcttt ttggcctact ttaaatactt taatttcttc atcgaaaaca
     2761 tcaatttatt actcaaagga ggaggactga attttattga agtcattatg cctataggta
     2821 tttcgtttta tacctttcag gccatttctt atgcggtgga tgtttacaga ggcgatgtaa
     2881 aagtgcaaca ggatatcggc aagctggctt tgtttatctg ccttttcccc caattgattg
     2941 ccggccctat attaaaatac aacgacattg cccatcagat cgattcccgc gaagtcaacc
     3001 ccgaaaaagt aactatgggt gtgaagcgtt ttattatagg gctttctaaa aaagtattgt
     3061 tggctaatac cctgggcgca gtggccgata aaattttcga cctgtcgccc gatcttgtac
     3121 agacttccat tgcctgggtc ggcagtttag cctatagctt tcaattattt ttcgatttta
     3181 gcggatattc tgacatggcc gttgggctgg gtttgatttt cggttttcac atcaaagaca
     3241 acttcaatta cccctatatt tcaaaatcca ttaccgaatt ttggcgcaga tggcatattt
     3301 ccttgtccac ctggttcaaa gaatatcttt acattcctct tggtgggaac cgggtgagta
     3361 agccccggaa ttacttcaac ctggccgtag tatttctttt aacgggattt tggcacgggg
     3421 ctgcctggaa ttttgtggtc tggggcattt ggcacggcct ttttattatc ttcgaaaaag
     3481 tcaccggctg gcacaaagaa aattcaagac agtggattaa tccggttaaa cacctttata
     3541 ccatattcgt ttttatcata ggctgggtca tgttccgggc cgaaaacctg agctatgcct
     3601 ggcaatacat taaaaacatg ttcggactgg ccggaaataa aaccatttta tacgaagcag
     3661 cctattactt tgataacatc gagataatcg cctttattgc ggccttactc tgttctgttc
     3721 ctttattttc gaacatgttg aacatacctc aggaacgaaa atggctcagg tggctggtaa
     3781 atgcctggtt actgtttttg tttattctct ccacctcggc aattgccgcc tccacttaca
     3841 atcctttcat ttatttccgt ttctgacgga ataatcgttt agtcgtagca ttaaaaactt
     3901 cgtgattttt atcggtttat tgtattatta ttttgcagcc ataaaaataa tgtataattt
     3961 tgcagccggt ttttgtattt ttattcaaaa gaaaaaaatg aagaacaagg ctaatcgtat
     4021 gaaaagcata cgaaaaattg tacatgaaac gcgcatcagc agccaggagg aattactgaa
     4081 gctacttact gaagaaggtt tcagccttac gcaggccact ttgtcgagag atctcaaaca
     4141 actcaagatt gccaaaatgc ccgattacga aggtggatat atttacgttt tgcccgaaga
     4201 atcttcccct ttaagtagaa ctgtacgcat gaagtcgcaa gacgtacact ttattcagtc
     4261 cggctttgaa tcgcttgctt tttgcggaca ttttgccgta atcaaaacca agcctgccta
     4321 cgccggagga attgccgccg aaatcgacaa aaaaggctcg aaacttattt tgggaaccat
     4381 cgcaggagac gacaccattt tggtaatccc caaagaaaag accgatcgac aaagccttat
     4441 gcaagcctta tcggagatta ttccactttg atttacaatt caccctatta aagaatttta
     4501 ccctgttcta aatgcgaaaa attttgtctg aacaggtttg tttaaagatt gaaatggata
     4561 aaaaatttga gatcgtagtt gcaaacgcat cacatttgca gtacgttgac actatattga
     4621 acactatcga ggaagcagct aaaataaggg gaaccggtat agccaaacgt tcaccggaat
     4681 acctgactca aaaaatactc gaaggaaaag ccatattagc tctggtcggc gacgtctttg
     4741 ccggcttttg ctacatcgaa acctggggca acaaacaatt tgtagccaac tccggtttga
     4801 tagtggtaga acaatacagg caacttggcc tggccaaaaa aatcaagaaa aaagccttta
     4861 agctatcgcg caccaaattt ccggacgcaa aattgtttgg actgaccacc agcgcagccg
     4921 taatgaagat taattacgaa ctcggctaca gacctgttac cttcgcagaa ctaaccgacg
     4981 acgaggcctt ttggaaaggt tgtcagagtt gcgtaaacta cccgattctg gccgaaaaga
     5041 accgtcgctt ctgcatgtgc acagccatgt tgtacgatcc taaagaacat aaaagacacc
     5101 ggcgcattta agcgggcaga tttgatgcag gcatatttaa aaacgcgcca tttcgaacga
     5161 caatctaaaa aacaatataa taagatgaaa gaaaaagtag ttttagcctt cagtggagga
     5221 ttggatacct ccttctgcgt aaaatacctc agtatcgaaa agaatttcga agtctatacc
     5281 gccgttgcca acacaggagg cttctcgcag gaagacctca aggtaatcga agataaggcc
     5341 ctccggctgg gagccgtaaa gcacgtaagc ctcgacatca cgcaggaata ttacgaaaag
     5401 agcattaagt acatggtttt tggcaatgtc ttacgcaacg gcacttatcc catctcggta
     5461 agttcggagc gcatatttca ggccatggcc attattgaat acgccaaatc cgtcggcgcc
     5521 aaatacgttg ctcacggcag tacgggggcc ggcaacgatc aggtacgctt cgatctgact
     5581 tttcagatac tggctcccga aatcaaaata atcaccccca cccgcgacat gatgttgact
     5641 cgcgaatatg aaatcgatta cctaaaaaaa cacggttacc gggccgactt taccaaaatg
     5701 gaatattcca tcaacaaagg gctttgggga acctccatag gtggcaagga aacccttcat
     5761 tccgaacaga cactgcccga gtcggcctac cccagccaaa taaccaaaga gggcagcgaa
     5821 gtactctgta ttgatttcca taaaggcgaa atctcgggag tgaacggcac tgcttacacc
     5881 gacaaagtga aagccattca aaaagtggaa gaaattgcct cggcctacgg cataggacgc
     5941 gacatgcaca tcggcgacac catcatcggc atcaagggac gggttggttt cgaagccgcc
     6001 gcgcccatac tcatcattca ggcgcacaaa atgctcgaaa agcatactct gaccaaatgg
     6061 cagcaatact ggaaagacca aatcggcacc tggtacggaa tgttcttgca cgaagcacaa
     6121 tacctggagc cggtgatgcg cgacatggaa gctttcctca gcagcagcca gggcaatgtc
     6181 tgcggcagag tcagcatcag gctcagaccc tacagcttca gcctgattgg agtagacagt
     6241 ccttacgatc tgatgaaaac cgatttcggt gaatacggcg aagtaaacaa agcctgggat
     6301 gccaacgata tgaaaggctt taccaagata ctgggcaacc agatgaaaat ttattacaac
     6361 gtacaagaca gagtaaacaa aaaatgatca gagtaggaat tattggcgga gcgggctaca
     6421 ccgccgggga gttgctgcgg gtgctgataa atcacccggg agttgagctt gtgtttgcac
     6481 acagcaacag caacgccggc aagcctgtta cctctgcaca cggaggactt tacggtgaat
     6541 gtgatcttgt atttaccggc gaattgactt tcgacaatat cgatgtcttg tttatgtgct
     6601 ccgctcacgg cgacagtaaa aaatttctcg aaagtcattc tcttccgccc aaactcaaaa
     6661 taatcgactt atccaccgat tatcgcgacg aaagcgatgg ttttgtttac ggtttgcccg
     6721 aattacaaaa agagaagatt cgtagggcag acaaagttgc caatccgggc tgctttgcca
     6781 cttgcattga actggccctg cttcccctgg cggctgccgg tctgttgact tcggatatac
     6841 acgtcaatgc cattaccggc tccaccggcg ccggcgtaaa gccctcgtcc acctcacatt
     6901 tcagttggag gaacaacaat atttcggttt ataaagcttt tgaacatcag cacctcaaag
     6961 aaatcaggca atcgctgagc gccttgcaaa aaggattcaa agccggcatc catttcattc
     7021 ctctgcgtgg taatttttcc agaggcattc tggcaacggc ctacaccacc tgcccctgga
     7081 gcctcgaaga ggcgataaaa gcctatacca agttttaccg cgaggctcgt ttctgtttcg
     7141 tctgcgaaga aaatcccgac ctcaagcaag tggtcaacac caacaaatgt ctgttgtatc
     7201 tcgaaaagca cggagatcaa cttttgatta tctctgtggt ggacaattta ttaaaaggcg
     7261 cttcagggca agcggttcaa aatatgaatc tgatgtttgg cctggacgaa agggcaggct
     7321 tgaatcttaa gagtacgggg ttctaatcct ttatgcgaag gcctttgctg aggcttattt
     7381 tggatggatt tttgatttgc tgtaaatctt ttattttaat ttgcccggcc aaaattcgct
     7441 ttgcttataa caaaactgga agaaaatgaa tttatttgat gtttatcccc tatacgacct
     7501 cgaaattgtc agtggcaaga actgctgcat ttacgataaa caaggaagag aatacttaga
     7561 cctgtacgga ggtcacgccg tgatttccat cgggcattcg cacccgcatt atgtacggag
     7621 catctgcgaa caagtcgctc gcatatgttt ttactccaat tccgtggtaa acagcctgca
     7681 gaccaagttt gccacccgtt tgggaaagat ttccggctac gaagagtaca gcctcttttt
     7741 ggtaaattcc ggggcagaag ccaacgagaa tgccctcaaa ctggcctcat ttcataatgg
     7801 acgccgcaaa gtagtcgcct ttcgcaaggc ttttcacggt cgcacttcgg cggctgttcg
     7861 cgtcaccgac aaccccggca tagtggctcc ggtcaatcag ggcatggaag tagaattctt
     7921 ggcgctcaac gacataccgg ccatgcaaaa ttgcttatcc aggggagatg tctgcgctgt
     7981 cattatagaa ggcattatgg gagtaggcgg tgttcatatt ccccagccgg cctttttgca
     8041 ggctttgcgc aaaacctgta acgaaacaaa aaccgtcttg attctggacg aaattcaatc
     8101 gggctacggg cggagcggcc ggttttttgc tcatcaatac gccggaatac ggccggatat
     8161 gatcaccgta gccaaaggca ttgctaacgg tttccccatg agcggactca ttattggccc
     8221 cgaatttaaa gcggttcacg gcatgctggg aaccactttc ggaggcaacc acctcgcttg
     8281 cgccgcagct ctggcagtac tcgacgttat ggaacaggaa aatctgatcg gtaacgcagc
     8341 ccgtgtaggc gattttttga tgaaaaaatg caagactata ccgcaaatag cagaagtgcg
     8401 cggtctggga ctgatgatag gcctggaatt cgacaagccc atcaaagaac tcaggaaaga
     8461 actgctcttt gaacacgctg tatttaccgg ggtaagcgga agcaacgtta ttcgcctgtt
     8521 gccccctttg agcctgagca tggagcaggc cggtgttttt atcgaaaaac tcgtactggc
     8581 tttaaaaacc ttttaattaa caaaataagg agcagttata atgaaatcaa tcctctcgct
     8641 gaggaatacc atcgttcgtc aacgcttcaa taaatataaa tctttagctt aacggctgtc
     8701 attaaaaagt tcaattatga aaatcggaat tataggagta ggcaacatcg gtaaggccat
     8761 cgtgtcaggc ctggccaaat cggcctttgt gcccgaagag aacatcgtta tttcggacat
     8821 cgaaaaatca aatctggaac tgattaaaag ttctttcccc aagattcaaa gttcaaccga
     8881 aaacaaaaca gctgctcagg cagacctcgt tattgtggct gtcaaaccct ggctggtggg
     8941 aggagttctg acagaaattg ctcctttgct tgagacttcg agaccgattt tggtcattgt
     9001 ggctgccgga gtaagttttg aacagatcta cgacaaatta cccgaaaatc tttcgaatct
     9061 gccctgttac cgctttattc caaacacggc catcgccttg agagaaagca tgagtctgtt
     9121 gtcggccgcc aatactactc ccgaacaaga caagttgatg atgggtatgc tcaaaaaatt
     9181 gggaaaagca gtactcattc acccggatat gatggcggca ggtacttcgt tgacttcttg
     9241 cgggacgggc tttgcgttac agtttatcaa agccaacgtt caggtggcca tcgagatggg
     9301 tttcaccccc gagcaggcca cggaattgat agcccaaacc gtcaagggag ctgctgaatt
     9361 gctgctaaac aacaaaacca ctccagaagt ggaaattcgc aaagtcacta ccccgggagg
     9421 tatcaccatc aggggactga acgaaatgga ggcctcgggt ttcacggctt ccgtcatcaa
     9481 aggcattaag gccagtttac tctgagacta tgtatattta tcgtcgcgtc gttgttaaaa
     9541 tcggcagtaa tgtcctcacc cgcaaagacg ggaccctgga tgttacccgg atgtctgctc
     9601 tggtcgatca gatggctgaa cttcacaaag ccggtctgga ggtgattctg gtttcgtccg
     9661 gggctgtggc ggcagggcgc agcgaaattc aggcctcgcg caaactggga caagtagagc
     9721 aaaggcagct ttattcggcc atcgggcagg ccattctgat caataggtac tacgatcttt
     9781 tccgtgatca caacatcgtg tgcggacaga tattaacaac caaagaaaac tttgcagacc
     9841 gccggcatta cctcaatcaa aaaaactgta tggaggtcat gctcgaaaac aaggtaattc
     9901 ccatcgttaa cgaaaacgat accgtctcgg taaccgaact gatgtttacc gacaacgatg
     9961 agctttcggg cctgattgca ggaatgatga atgccgaagt cctgcttata ctcagcaacg
    10021 tggacggcat cttcaaagga caccccgacg atcccgaagc ggaattgatc gaagagattt
    10081 tgcccaaaca agaactacga caatacatca gtgccgaccg ttcatccacc ggcaggggag
    10141 gtatgcgcac caaatacaac atcgcctcca agctggccaa agaaggtatc cgggtaataa
    10201 tagccaacgg gaaaaaagat aacatcattc ccgacttatt cagccccaaa ccccgcaata
    10261 aacacagctt gtttctgccg gccgaaaaaa cctctgccat caaaaaatgg attgctcatt
    10321 ccgaaggttt cgccaaagga gaagtacata taaaccaagg ttgcacggag gccttgacac
    10381 aggcccaggc cagcttgttg cccgtgggca tagaacgcat cgagggtgag ttcgaaaaag
    10441 gagatattgt acgggtcatc gaccacgaag gtcgtcagat tggtgtgggt atgatccgtt
    10501 gcgacagtga tgctgcccgc aacatcatag gatccaaggg acaaaaagcc cttattcatt
    10561 acgattattt gtatctggaa taatgagttt ggaaaataaa ttcaaagctg taaagcaagc
    10621 ctcagctctg ttgaacgatt taagtgacat agccgtacgg caattactaa cagacctggc
    10681 tgccgaagcc gaagcgcaaa tcccctatct tctgactgaa aatcagaaag atttggaccg
    10741 gatgaatccg gccgatccca aatacgaccg gctcaaactc agcgccgaac gtattcacgg
    10801 catcgcttcc gatatgcgtc aggtggccgc cctgtcttcg cctctgggcg agattttaag
    10861 caaggagttg cgtcccaatg gtatgcttat acaaaaaatc agggtgcctt tcggagtgat
    10921 cggcatcatt tacgaagcca gacccaacgt tagtttcgat gttttttcgc tctgcataaa
    10981 aacggggaat gcctgcatac tcaaaggcgg ctcggacgcg gaatattcca atacggccat
    11041 cgtgcaactg atacgacaac aattgctcaa acatggtttt ccggacgatg cggtcttact
    11101 tttacccccc gaccgccggg ctgccgacga acttatgaag gcaaccgctt atgtagatct
    11161 gatcattccc aggggcagtc agtctttgat cgaccacgtg cgcagtcatt cgctggtgcc
    11221 ggtcatagaa accggtgccg gtatttgcca tacctacatt gacagctcgg ccgacataca
    11281 aaaggccgca accattgtgc ataacgcaaa aacccgccga gtgagtgtct gcaatgccct
    11341 ggactgcatg ttgttgcatc gctcccgttt gcaagacctg cctgaaatct gccttaaatt
    11401 gcaggacgag caggttgtga tttacgcaga tcccgaggcc ctggcagccc tcaaaagcca
    11461 ttaccccgga catttgttac aaccggccgg cgaaaagcat tacggcacgg agtttctgga
    11521 ctataagatg gccgtcaaaa cggtgaaatc cacagaggaa gccatcgaac acatagcccg
    11581 ctattcttcg ggacacagcg aggctatcgt ctgcgaagat accgaacagg ctcaactctt
    11641 ttgcaaaaaa gtagacgctg cctgcgttta tgtcaatgtg tcgactgcct ttaccgatgg
    11701 cgcacaattc ggcctgggag cagaaatcgg catcagcaca caaaagctgc acgcccgtgg
    11761 acctatggcc ttaaaagaaa tcactacata caaatattta attaccggta acggacaaat
    11821 acgttcaaaa taatgagaca ttttaccagc attaccgacc tgggcgacct caaagctgca
    11881 gtaagcgagg cccggcaaat caaagcccgg ccttttgcct ggcaaaacct gggcaaaaac
    11941 aagaccctgt tgatgatctt tttcaattcc agcctcagaa cccgactgag tacccaaaaa
    12001 gccgctttaa acctgggcat gaatgttatt gtgctcgatg tgaatcaggg agcctggaaa
    12061 ctcgaaactg agcgtggtgt cgtcatggac ggagataaat cggaacatct actcgaagcc
    12121 attccggtta tgggctccta ctgcgacatc atcggcgtac gttcctttgc ccgctttgaa
    12181 agcaaggccg acgattattc cgaaaagata ctgaatcagt ttatacaata ttccggcaaa
    12241 ccggttttca gcatggaggc cgccattcgc catcccctgc aaagctttgc cgacctcatc
    12301 accatcgaag aattcaaaga aagggcgcgt ccaaaggtgg tcctgacctg ggctcctcac
    12361 cccaaagcgc tacctcaggc tgttcccaac agttttgccg aaaccatgaa tctctgcgat
    12421 tatgaattcg tcattacaca tccccggggc tacgagcttt ccccggaatt ttccggcaaa
    12481 gccgcaatag aatacgatca aaacaaagcc ctcgaaggag cagactttgt ttacgccaaa
    12541 aactgggcag cctttagcga tccgaattac ggtaaaattc tgagccggga tcgttcctgg
    12601 accatcgaca gcaaaaaaat ggctctgacc aacaatgcac gctttatgca ttgcctgccg
    12661 gtgagacgaa acatgatcgt cagcgacgag gtgatcgaag gcccccgttc ggtggtgatt
    12721 cccgaagccg ccaaccggga attttcggcc caaacggtgt tgaaacgcat attggaaggt
    12781 ttagattaaa tccggattgg ctctgatacg attccggatg ttgacgggat ttaaacggat
    12841 ccgaacttaa ccctcaggct taaagaaatg aagcggctgc ttcgttaatt ccgtcgaaaa
    12901 gcctaccttt gcggctcaaa tcaatcaaaa tggatacagt tttaagcgga atacgctcta
    12961 cggggcatct gcatttaggt aattattttg gcgccttgcg caattttgtc aggatgcaac
    13021 acgaaaatcg ttgcttcttt tttattgcag attatcattc tcttaccacc cacccggatc
    13081 ccgggtccct gcacgctaac gtaaaagata tcttgtgtga atacctggct gtaggcatcg
    13141 atccggaggt tgccactatt tacgtgcaaa gcgacgtccc cgaaaccgta gaactctatc
    13201 ttttgctcaa tatgcatgct tatatgggcg aattggccaa aacaacttcg ttcaaggaaa
    13261 aagcccggaa acaacccgaa aacgtaaacg ccgggctgct cacttacccc actctgatgg
    13321 ctgccgacat cttgcagcac aacgccgaca aagtgcccgt gggcaaagat caggagcaac
    13381 atctggaact tacccgccgt tttgcccggc gctttaataa tttgtacaag gtcaattttt
    13441 ttactgaacc ccaaccttat aatttcgatc aggaactcgt aaaaataccc ggcctggacg
    13501 gcagccgcaa aatgggtaaa tccgagggca attgcatttt tttatgcgaa gatcctaaac
    13561 ttataaagaa aaaggtaatg aaagcactga ccgatgccgg gcccacggag cctcattcag
    13621 ccatgcccga atcgatcgaa aatctcttcg ttcttctgaa aagcgtttcc agccccgaca
    13681 cagtggccta tttcgaggag caatggaaca attgcagcat ccgttacggc gacctcaaaa
    13741 aacaactggc cgccgacatc attgccttta ccactcccat tcgcgaacgt tatttagaca
    13801 tatgcaacaa cgacgaatac ctgcataaag tagctgtaaa cggtgccgag aaggcacgcg
    13861 ccagcgctgc cgccaccttg cgcgaagtgc ggcacattat gggtataagg cctttttaat
    13921 tacgaatgaa tcaacaaacc aaaagtttta ttcatagttc ggcattcatg gctataatag
    13981 cctgcttgct ttggtctacc gcttttgtcg gcgttaaaat cggattgaat tacatgagtc
    14041 cactcctgtt tgccggactt aggttttcta tcgccggtat cctgattttg ttgtactacg
    14101 gcaagtttag ggcctacttt caagctatta aaacacattt ctggtacatc ctgttaattg
    14161 cttttttgca aacagtattg atgtatggct tgttttatac cgccatgaat atggtgccgg
    14221 gctctatggg agccatgatc aacggcacgg gtcccttgtt tgcggccatt gtggcgcatt
    14281 tgtttattaa agacgataaa ataacttggc gcagtggtgg cagtattatg ctgggaatcg
    14341 taggtatagt gatcatcaat ataggcagac agaccggagg caacgcggga ggactcgaaa
    14401 taatcggcgt tttactctta attgccaaca atcttgtaag cggactctac aacgttatag
    14461 tcaagaaaag ccggcgaaat atttcgatgc tcacactgag ttctgcttcg ctgctgattg
    14521 gagggatcgt gttgattctg atttccattc ccttcgaaac tgcgccggcg aatctgaatt
    14581 ttccgcttga attttggctg gctctcggct ggttgagttt cttatcggct gcagcctttg
    14641 ccttgtggtt cactttactg aaacgccccg gagtaaaagt atcttacctg aacagttgga
    14701 aattcatcat tccggtgttt ggggccatac tcagttggct aatattgccc ggtgaatacc
    14761 ccgacattta ttccatttcg ggtgtgatca taattggagc ctcactgctc tggatgaaca
    14821 atgaagccct taaagatatg tttggcaaat ggctggccaa aattaaacct taaaactatt
    14881 ggtcatattg ccttcttttg ccttttcact gaccatcgga agcttcggct cccggctcca
    14941 tacctctgcc tctaccaaaa ccccggcctc ttccgcggcc cataccgcct ccaaacccaa
    15001 ggcctcttcc gcggcccata ccgcctccaa acccaaggcc tcttccacgg cctaagccgc
    15061 ctcccaaacc gcgacctctt ccgagatcct tgccgtcttc aaaattatca gtaaaacccg
    15121 ggctgtcgtg ccccttgcaa aaacctaatc ctcttccggt catgggacct tgtccctttg
    15181 gacctgtttt gtcacctctt ggcataattg taaattttta aaatgatact tatttttttc
    15241 tgatacaaat atgcctatta ttatgaacat atgttcattt gacccataag atttaatatt
    15301 atttaagatt attgttcgtt atgggaagga aatgaccgat taaagcccca ggaagagcaa
    15361 tatcgttccg attacaagac caatcagtac actgatgccc ttaatcagga agaagtcctt
    15421 acggctttcg gccaataccg gcaacatgcc gtgaccatcc tgcactatag aactggccaa
    15481 aagaatacta aaaggaatca gttgctggga gaacaaggta acaaaaacca aatgcggccc
    15541 agattcggga atgatgccta tcaatacggc cgctaacaaa atcaagtagg gattgttttc
    15601 gaccaatgta cctaatagtt gttctgtttg tacgaaataa agaacgataa aagcccccca
    15661 agaccagagc aaaatgcgta aaaggtgctt cttgatgatg tggttgtaca aatgttcttt
    15721 gagaaaatgt tccgggacgg tcaaaaaaac aaacaagaga aaaagactgc cggccagcaa
    15781 ggtaattttc tcccaacccc agccttcatg gccaattacg cccaaagcaa gcagcagcaa
    15841 gaacacaaga gtagctagca gtaaaagact acgttcaaaa gtaattttct tgagttgagg
    15901 tagtataatg ctcctgtcaa agcatttgca actatcctct tgatggacct caaagccgta
    15961 ttgaggcccc gccggttctc tttctttttt acagaacaga tagattatcc aaccggtcaa
    16021 gagggctata ataaacagta ttccatgcaa caacaaagct ttgccgggga aaagggcgaa
    16081 catcacaaag gcttcgtcgc cgctggtagc aatcatggct gccactacgg cagccaggcc
    16141 tatcattctg tgcgtgtaca aagacaccac cgcaaaagca cccatgcaac cgggtataat
    16201 tcccaggatt gttgccatta aaatttgcgt cagaggagaa cggcgtaatt tttcggccca
    16261 gcgattttta ttttgcacat taatatattc aacgaccacc atcatcagca tgacaaagcc
    16321 cgttatcata agtgcacgca tgagtgtttc aaaaaggagt tcccgcatat ttcccgtatt
    16381 ttttgaggcc caaatttagt caaaatctgc tgtgtggatt tattcgtatt acaaatttta
    16441 tttgtttgat gtggataaac tgaacgattt taaaaagaac aaattagagc ttgaagaatt
    16501 cttttatatt tgcgctttgt aaaatattct actattacac tttgagacat taaactctat
    16561 gaaatacctg ataatcggcg gtgtggcggg tggagccact acagcagcaa ggctaagaag
    16621 gctggatgaa aaaagcgaaa taatcgtttt tgagcgtggt gaatatattt cgtatgccaa
    16681 ttgcggcctg ccctattata tagggggtat catcaaagac agagatcaac tgtttgtaca
    16741 gactccggaa tcttttggcc aacgatttaa tttggaagtc agaaatttat ccgaagtgct
    16801 ttccatcgac agacgcaaga aagaagttct ggtgaaagat attagaaacg gaagtgaata
    16861 tagggaaagc tacgacaaac tcattctttc gcccggcgct gaacccataa aacccatgtt
    16921 gcccggcatc aatcaggaag gcatcttcac tttacgcaac gtacccgaca ccgataagat
    16981 taaacagtac attgagcaaa aacaagcccg tcatgctctg gtagtcgggg ccggatttat
    17041 tggtttagaa atggcggaaa acctgcatca cgccggtctt aaagtgacca ttgtcgaaat
    17101 ggccgaacaa gtgatgactc ctctggacta ttctatggct gc
//
LOCUS       DAOSGL010000008        16826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_2778_length_16826_cov_4.701509, whole genome shotgun sequence.
ACCESSION   DAOSGL010000008 DAOSGL010000000
VERSION     DAOSGL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16826
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2778_length_16826_cov_4.701509"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1035
                     /locus_tag="PKY68_00595"
                     /pseudo
     CDS             1..1035
                     /locus_tag="PKY68_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661442.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
     gene            1120..3126
                     /locus_tag="PKY68_00600"
     CDS             1120..3126
                     /locus_tag="PKY68_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOH23572.1"
                     /translation="MVSVENIKVEFGDFILLNSISFVINKKDRIALIGKNGAGKSTLL
                     KILAGLQAPGEGKLGIAKETRVAYLPQVMSCPDDCNLIQETEKAFTHIIKLQAEVEHI
                     QHQLSVRKDYNSDEYHQLIEKLDQKSQALSMTEAGNYMAEIEKTLLGLGFERSDFDRP
                     TEEFSGGWRMRIELAKLLLQKPDLLLLDEPTNHLDIESIQWLENFLAQNAKAVIVVSH
                     DRAFLDAVCTRTIELSLGRIYDYKLPYSRFVEFRKEMREQQLRSYENQQKQIQDTEAF
                     IERFRYKATKAIQVQSRIKQLEKMERIEVDEQDNSHLNLRFPPAPRSGSFPVIIEQLS
                     KSYGEHLVIRDVHLSLRRGEKVAFVGRNGEGKSTLVKCILEEIPYEGSIKLGHKVKIA
                     YFAQNQPQLLDENLSVFETVDKAATGDVRTKIRDILGAFMFGGEASDKKVRVLSGGER
                     SRLAMIRLLLEPANLLILDEPTNHLDMPSKDVLKAAIKAFDGTVILVSHDREFLDGLV
                     EKVYEFSHQKVIEHLGDIRQFLEKKKLDNLRQLEQNPLNAAPGNNKTTVEIKAANAFT
                     GNTDRKEKQENSNAPIANNDYAERKALNRKIKKLERLVQEREDEVSKLEKQLESIQNE
                     LNTPQGACRPELHEQYIREKKALDNALSYWEMAATELESLKINS"
     gene            3135..5162
                     /locus_tag="PKY68_00605"
     CDS             3135..5162
                     /locus_tag="PKY68_00605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOH23573.1"
                     /translation="MKKIVFTLIIAGLSMSSCIDKKTTGVPGIDLTDLDTTMSPAEDF
                     YQYACGGWVAKNPLPAEYARYGSFDRLAENTQKQLQELVLELDASTQPEGSVARKIAM
                     LYHSAMDSVKLNAEGIEPIRKDLEQIKALENKEALIGHLANMQKRGIWPFFVLYAGAD
                     DMNASMNILHTYQSGLGMGERDYYLSPEERNRELREKYVLHVEKMFVLAGFTTTEAQK
                     AASDVMAIENRLAAASLSREDLRDPYKNYHKMSLEELSRLAPQMDWNLYFESLGDKEI
                     KELNVAQEKFIAEVVAVIHETELNALKNYLGWNLINTAANFLSDDIALQNFDFYGRTL
                     SGSEEMRARWKRALGAINGTLGEALGQVYVEKHFPPRAKERMLKLVDNLQLSLGERIS
                     SLEWMSEETKQKALEKLGTFHVKIGYPDKWKDYSSLEIKEDSYWENICRASLFSFNEG
                     MDKVGKPVDKEEWLMNPQTVNAYYNPTTNEICFPAGILQPPFFNMDADDAVNYGAIGV
                     VIGHEMTHGFDDQGSQYDKDGNLANWWSPEDSEKFAERTAVLEEYFNKIEVAPGVFAN
                     GKFTLGENIADHGGLQISFHAYTKTDEYKQNQSIDGLTPAQRFFIAYAHVWANNIREQ
                     EILRRTAEDPHSLGYWRVNGALPHIDAFVEAFNLKEGDAMFLPKEQRASIW"
     gene            5166..6422
                     /locus_tag="PKY68_00610"
     CDS             5166..6422
                     /locus_tag="PKY68_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOH23574.1"
                     /translation="MKQVLQEVQKINIKDYDYGLPDERIARYPAVPRDSSRLLIYDGK
                     NLSQDIFRKIADHLPSPALMVFNNTKVIHARLLFHKKSGAGIEIFCLEPAQPADYIQS
                     FQSLGSCEWYCLIGNSKKWKEGSLNKEIRAGGLTIQLQATRTGQNGRLQGIRFDWQCP
                     QKTLQFSEVLELAGIIPIPPYLKRESESSDEKDYQTVYSKIKGSVAAPTAGLHFSEEE
                     LKNLDRQHILRRELTLHVSASTFQSVKSDRLREHPMHLEFFSVERTLIEDIVRNKNPL
                     IAVGTTSVRTLESLYWFGLQLLENKAAAPEDPVVDQWFPYLDHQTYTTEEALTALLDY
                     LDRNKLDRFCASTRLLIAPGYHFRLVDAMLTNFHQPQSTLLLLVAAFVGRDWQRIYRY
                     ALDEGFRFLSYGDSSLLFPGNQQTKT"
     gene            6501..8477
                     /locus_tag="PKY68_00615"
     CDS             6501..8477
                     /locus_tag="PKY68_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH23575.1"
                     /translation="MRHFIGYILVLFLSALASCSVSKDIRKADKHYDYGEYNAAAVIY
                     TKAYRRIPAVEKNVRAEVAYRQGECYRFINQSVKAENAYMKAIRYGLPNDSVLLHYAE
                     VLRKNAKYEAAEIQYRQFLENHPGDLQASYGLESCLLAREWKNKPKKYAVNRVQELYL
                     KRGNFSPLLVPAEYKTLLFTSSSIQKEGKKPSKITGLPDNDFFITRLDQNEKWEKPLP
                     IEGAINSEFDEGVGCFDPDGKVLYFTRCVNKSDSTLSGSSAEIYRSVRSGEQWSEPEK
                     LNIFRDTNAVFAHPAISPDGLYLYYVSDMKGGYGGKDIWRSAFYGKSFGPPENLGPQI
                     NTPGDEMFPTIRTDGSLYFSSNTLPGLGGLDIFKAVYNEEDSSWTVENVYDLNSSADD
                     FGITFFGKKEKGLFSSNRNEPRGWDKIWSFGEPEQLTQISGWVTDRFGEPIPEATIRI
                     VNDKGTNTKVVSRRDGSYSYQVEKNAEYVMLGTCRSYLNYSNQFVTTDKDSNYVVNFS
                     LTSLYLPVRIENIFFEFDRWELRPESGPALQELLKLLLDNPHITIEIGAHTDRKGDEE
                     YNLKLSEKRAESVVNYLIKYGIDKERLSAQGYGKSKPAKVDKYMETNYPYLKEGSFLD
                     ESFVETLSLQQQEIADQINRRCEFKVLKTTYKLF"
     gene            8477..9271
                     /locus_tag="PKY68_00620"
     CDS             8477..9271
                     /locus_tag="PKY68_00620"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOH23576.1"
                     /translation="MKAYLDLLQRVLDEGVEKKDRTGTGAISIFGHQMRFKLSEGFPV
                     ITTKKLHLKSIIYELLWFLKGDTNVRYLQENGVRIWNEWADEDGNLGHIYGYQWRSWP
                     DYRGGHIDQISKAVDEIKNNPDSRRIIVNAWNVADLPNMNLPPCHMFFQFYVANGRLS
                     LQMYQRSADIFLGVPFNIASYALLLKMMAQVCGLEAGDFVHTLGDAHIYLNHLEQVRL
                     QLSRDPRPLPEMLINPEVKDIFSFKYEDFQLINYDPHPHIKGEVSV"
     gene            9315..9800
                     /locus_tag="PKY68_00625"
     CDS             9315..9800
                     /locus_tag="PKY68_00625"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOH23577.1"
                     /translation="METAIIAAIANDYALGLDNKLLCHLPADLKHFKELTSGHTVVMG
                     RNTFFSLPKGALPNRRNIVLSPDEETFTGCETAHSFPEVFELCQKDEKVFFIGGAMVY
                     SQAMEWVDSLYITWIHASFEADVFFPEINQDIWEEAEREDHFDEEPYPYSFVHYVRKK
                     D"
     gene            9781..10890
                     /locus_tag="PKY68_00630"
     CDS             9781..10890
                     /locus_tag="PKY68_00630"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOH23578.1"
                     /translation="MSEKKINTCSGFLIGLLFVLFSGLSFAQTAPYAEIAFEKAIHDY
                     GTIKESGYAHCEFEFSNTGNAPLEIRRVTASCGCTTPDYPKAPIAPGAKGIIKVAYNT
                     TGRPGPFNKSITVYSNATANPVIMLSIRGTVESNNNDAERLFPKTMGGLRLKRTMIPF
                     GDVLIGSIKTETIPVYNQSEAPIELSFNKVPAHMRVMISNSRLEHGNTGIITINYIPE
                     EARDYGQRQDFFYIVTDPAEKENPANIIIVSANIKEDFSRTGPLDKVPRASFSQTGID
                     LGKVSGTEKKTAELVLKNPGEKPLIIRKATATCNCIELKADRKEIAPGKESRVRIVFE
                     AGKLIGNLHYTLEFITNDPRQPITKIPLTVNVIAK"
     gene            10887..11972
                     /gene="meaB"
                     /locus_tag="PKY68_00635"
     CDS             10887..11972
                     /gene="meaB"
                     /locus_tag="PKY68_00635"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOH23579.1"
                     /translation="MKHPENDARYRGLTVNKGIDQPPIINPYSKKRSPRKQYSVEEYV
                     QGILDGNNVILSQAVTLIESLRPDHQETAHEIIERCLPYAGRSVRWGITGVPGAGKST
                     SIEAFGMELIRQGRKLAVLAIDPSSERSKGSILGDKTRMEALSVEPKAFVRPSPTGGS
                     LGGVARKTRESIVLCEAAGFDTIFVETVGVGQSETAVHSMVDFFLLIQIAGAGDELQG
                     IKRGIMEMSDGIVINKADGNNIDRANLAAAQYRSALKLFPMPDSGVRPQVLCYSAQQL
                     SGVAEVIEMVNRHIELFKTNGSFEQRRTQQSIYWMRQSIDESLRNHFYHKPEMAAMLK
                     EKEQEVLNNKKTPFSAARELLDFYYNS"
     gene            complement(11960..12853)
                     /locus_tag="PKY68_00640"
     CDS             complement(11960..12853)
                     /locus_tag="PKY68_00640"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOH23580.1"
                     /translation="MARIEIDKHSGYCFGVVKAIAKAEEHLDSKEELYCLGDIVHNNM
                     EVKRLEKKGMKTVHYDEFAGLKNKKILIRAHGEPPSTYKTAERQGLELVDATCPVVLN
                     LQRRVHKAYQEHKNDGTQIVIFGKPGHAEVNGLVGQTQNTAIVVEHLADLVKVDLDRP
                     VIMFSQTTKSLEEYRQLVEAITNEINPAVGFQHFDTICRHMSGRVLQVRDFAAANDLV
                     FFVSDAKSSNGKSLYKACKEVNDNTHFISSPGEVRTIDVAGFQRIGISGATSTPRWLM
                     EEVRREIEKKLQVSGESTQEL"
     gene            complement(12834..13535)
                     /gene="cmk"
                     /locus_tag="PKY68_00645"
     CDS             complement(12834..13535)
                     /gene="cmk"
                     /locus_tag="PKY68_00645"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOH23581.1"
                     /translation="MEKRIIVAIDGHSSSGKSSMARQLAQKQNYLYIDSGAMYRAVAL
                     YCLRKGWIKGTCLDQEALVEGLKDITISFSTTDKGLPRICLNGEEVENEIRGMEVSGV
                     VSLVSAVPEVRSYLVDIQRSLAENKGVVMDGRDIGTAVFPEAELKIFLTASADVRAKR
                     RYLELKEKGIEASYEEILHNIEMRDQMDLSRTISPLRKADDAIELDNGRMSIEEQDAW
                     LEARFNEAVYGKNRN"
     gene            complement(13539..14528)
                     /gene="porQ"
                     /locus_tag="PKY68_00650"
     CDS             complement(13539..14528)
                     /gene="porQ"
                     /locus_tag="PKY68_00650"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOH23582.1"
                     /translation="MRRGLLVLLMLVVVLLRIRAGEGVYRFLTLPVSSYAAGLGGTVL
                     SSPENDLNLSLHNPAALSGEMHNQLALGYMNYVADIHLGTAAYARKINERSIWSAALR
                     YVDYGRIISAGDNLPEGEFFVKDMAMSGGYAFMLSPRWRAGAGMHLVYSVLEHYTSLG
                     VAVDLGVQYANPEQKIWAGFTLKNLGSQFKPYDESYEALPWDVQLGFTKGLLHAPFRF
                     TLTAWGFNAWSYPYLDGTTGRVVDNDNFVGKAFKHLLIGLEFVPSDNFQLSLGYNVRR
                     KSELSLAQRTILTGFTAGFSMSVRKLRIGASFAQYHIAGNTLQMTLSTNLGIF"
     gene            14699..15382
                     /locus_tag="PKY68_00655"
     CDS             14699..15382
                     /locus_tag="PKY68_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOH23583.1"
                     /translation="MQVKKSARADLDKNSTNNFLMGLVIALGFLFIGFEYSNTEVTVQ
                     DIYTQSGEIEEEIMIEITRQYIPPPPPPPQTVHSDLIQIVEDNTLEEEVEWASIEDFP
                     DEAIVMTYGSGDITEEYDPDGEVFMFVEEMPGFPGGESALMKFINDNLRYPQLALENG
                     ISGRVICTFVIDGRGKVSDIQVIRSVDPNLDREATRVISMMPDWKPGKQRGKPVRVRY
                     TLPIIFKLQ"
     gene            15475..16452
                     /locus_tag="PKY68_00660"
     CDS             15475..16452
                     /locus_tag="PKY68_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOH23584.1"
                     /translation="MKSIQEIRTLIQNGIREIDFPDRPSGLYEPLKYVLSLGGKRLRP
                     LLCLSACNLYNEEALSKALPLALGVELFHNFTLIHDDLMDRSLVRRNKATVHTKWDEK
                     TAVLSGDALQIMAFQQIALAPEDKLKDCLDLFNQTAREVCEGQQLDMNFEMLDNVRKE
                     DYIEMVRLKTAVLLGCSLKLGAIIGGAPDNEADFLYDFGIHLGIAFQIRDDLLDVYGD
                     PDIFGKAIGGDIVNNKKTFLWIHSIETADNHTRSLFLNCMKSNYMDREEKIKMVTRFY
                     NRLGARESCEQQMLDHQQSALKALEGLDLDKKRLVTLSELAGKLTYRQV"
     gene            16470..>16826
                     /locus_tag="PKY68_00665"
     CDS             16470..>16826
                     /locus_tag="PKY68_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23585.1"
                     /translation="MPYRRLPNTDRARIRALNAATKQGDLVGFLDLAYSYKLKNDALL
                     ILSQIEQAYHEYNINLERQTTSSKSYLAHLKTARLYISHFIQVLNLSVLRNEIKKEHK
                     KLYRLDVNRQSIPDLST"
BASE COUNT         4819 a         4110 c         3805 g         4092 t
ORIGIN      
        1 tcaggcggac caaaacggta tggtagaagt tcagggtgta atgaaaagca tctgagaaat
       61 gcaaaaataa gagattgcaa gcgaactgtt ttgatgagta attaataaat aaagttcatc
      121 aaaaccggtt tccttcctcc cattattttt atgggtgtgg gtgccttgac cgacttcggg
      181 cccatgctgc gcaacctgag gctgtccatt tttggggctg cagctcagct gggtattttc
      241 gccgtcttga ttggtgccat tttgataggc tttactccaa aagaagccgc atctctcggg
      301 attattggcg gagccgacgg tccgacagcc atatttacta ccatcaaact ggctccgcat
      361 ttgctgggtc ccatcgccat agccgcttat tcttatatgg ccctggttcc ggtgattatt
      421 cctttggtag tcaaactcac ctgtaccaaa aaggaactcc gtatcaacat gaaagagcag
      481 gaaaagttgt atccctccaa aactgagata aaaaacctca gagcggtaaa aattattttc
      541 cccatagccg ttactacggt agtggcttta tttgtgccgg gggctgtccc actggtgggc
      601 atgttgatgt ttggtaatct ggtcaaggaa atcggcgcca acaccttccg tctgtttgat
      661 gctgcttcca acagcattat gaacgcagct accattttct tgggattatc cgtaggtgct
      721 accatgacca gcgaagcttt tttgaactgg accaccatag gcattgttgt cggcggtttc
      781 ctcgcctttg cactgtctat tgccggcggt gtcctctttg taaaagtagt caatctgttt
      841 agcaagaaga aaatcaatcc gctcattggt gccaccggtt tgagcgccgt tcccatggct
      901 gcgcgcgtag ccaacgaaat tgcactgaaa tacgatcgtc aaaaccatgt gctgcaatac
      961 tgcatggcca gtaatatttc cggtgtaatc ggttcagccg ttgctgccgg ggtacttatc
     1021 tcttttttgg gatagcatta gagtcaacat atctaaggga ggctgtactt tgagtatggc
     1081 ctctcttttt tctccatttt taatacctaa ctttgcggta tggtttccgt agaaaatatt
     1141 aaagttgaat ttggcgattt tatcctcctg aactccattt cttttgtcat taacaaaaag
     1201 gaccgcattg ccctgatcgg aaaaaacgga gcgggtaaat cgacgctgct aaaaattctg
     1261 gctggcctgc aagcgccggg agaaggcaaa ttaggcattg ccaaagaaac acgggtggcc
     1321 tacctgcctc aagtaatgag ctgtcccgac gattgcaacc tcattcagga aaccgaaaaa
     1381 gcttttactc atattataaa actgcaggcc gaggtggaac atatccaaca tcagctcagt
     1441 gtaaggaaag attacaactc ggacgaatac caccagctta ttgaaaaact agatcagaag
     1501 agccaggcat tgagcatgac cgaagccggt aattatatgg cagagatcga aaaaacctta
     1561 ctgggtctgg gatttgaacg cagtgacttc gaccgtccta cggaggaatt cagcggcggc
     1621 tggcgtatgc gcatcgagtt ggccaaatta ttgctgcaga aacccgacct gctgttactc
     1681 gatgaaccca ccaaccacct cgacattgaa tccattcagt ggctcgaaaa ttttctggcc
     1741 caaaatgcca aagccgtcat tgtggtatcg cacgaccggg cttttctcga cgctgtttgc
     1801 accagaacca tagaactgtc gctgggcaga atctacgact ataaattacc ctattcccgt
     1861 tttgttgagt ttcgcaaaga aatgcgggaa caacaattgc gcagctacga aaaccaacaa
     1921 aaacagattc aggataccga agcctttatt gaacgctttc gctacaaagc caccaaagcc
     1981 atccaggtgc aatcgcgtat taagcagctc gaaaaaatgg aaagaatcga agtggacgag
     2041 caggataatt cgcatttgaa tttgcgcttt cccccggctc cccgcagcgg cagctttccg
     2101 gtaattatcg aacagttgag caaatcctac ggcgaacacc tggttatccg tgatgtgcac
     2161 ctcagcttgc gccggggtga aaaagtggcc tttgtgggcc gcaacggcga aggcaaatct
     2221 actttggtga aatgcatcct cgaagaaatt ccctacgagg gcagcatcaa gctggggcat
     2281 aaagtcaaaa tcgcctattt tgctcaaaac cagcctcaac ttctagacga aaatctcagc
     2341 gtcttcgaaa ccgtcgacaa ggcggcaaca ggcgatgtca gaacaaaaat ccgcgatatt
     2401 ctgggagctt ttatgtttgg aggcgaagct tcggacaaaa aagtcagggt actgtcgggg
     2461 ggagaacgca gccggctggc catgatccgc ctcttgttgg agccggccaa cctgctcatt
     2521 ctggacgaac ccaccaacca cctcgacatg ccctccaaag acgtactcaa agcagccatc
     2581 aaagcttttg acggaacggt aattctggtg tcgcacgacc gggagtttct ggacggcctg
     2641 gtagaaaaag tatacgaatt cagccatcaa aaagtaatag aacacctggg agacatccgt
     2701 caattcctgg agaaaaagaa actggacaac ctgaggcaac tggaacaaaa tcctttgaat
     2761 gccgctccag gcaacaataa aacaaccgtt gagataaagg cggccaatgc ttttacgggt
     2821 aacaccgacc gcaaggaaaa acaagaaaac tccaatgctc ccattgcaaa caacgactac
     2881 gctgagcgca aagctctaaa ccgcaaaatc aaaaaactgg aaagactcgt tcaggaacga
     2941 gaagacgaag taagcaaact cgaaaagcaa ctcgaatcaa tccaaaatga actaaacact
     3001 ccccagggag cctgtcgtcc ggaattgcac gaacaatata tacgagaaaa aaaggcactc
     3061 gacaacgccc ttagttattg ggaaatggct gccaccgaac tggaatctct aaaaatcaat
     3121 tcataatatg agcaatgaaa aaaatagtat tcaccctaat tattgcagga cttagcatga
     3181 gtagctgtat agataaaaaa acgaccggtg ttccgggtat tgatttgacc gacctcgaca
     3241 ctaccatgtc gcccgccgaa gatttttacc aatatgcctg cggcggttgg gtcgctaaaa
     3301 atccgcttcc ggccgaatac gcacgttacg ggagcttcga ccggttggcc gaaaataccc
     3361 aaaaacaatt gcaggaactc gtgttggaac tcgacgcaag cacacaaccc gaaggcagcg
     3421 ttgcccgcaa aatagccatg ctctaccaca gtgctatgga tagcgttaag ttgaacgccg
     3481 aaggaattga gcctatccgt aaggatctgg aacaaataaa ggcactcgaa aacaaagagg
     3541 ccctcatcgg gcatctggcc aacatgcaaa aaagaggcat ctggcctttc tttgttttat
     3601 acgccggtgc agacgacatg aacgcctcga tgaacatact gcacacctac caaagcggcc
     3661 tgggtatggg cgaacgcgat tattatctga gccctgagga gcgtaacagg gaactgcgcg
     3721 aaaaatacgt cctgcatgtt gaaaaaatgt tcgttttagc cggattcacc acgacagaag
     3781 ctcaaaaagc ggcctccgat gttatggcca tcgaaaatcg cctggccgct gcctcgctga
     3841 gccgagagga tttgcgcgat ccctacaaaa attaccacaa aatgagtctc gaggaattga
     3901 gcagactggc ccctcaaatg gactggaatc tatatttcga aagcctgggc gataaggaaa
     3961 tcaaagaact caacgtggcc caggaaaaat ttatcgccga agttgtagcc gtcattcatg
     4021 agacagagtt gaatgcactt aaaaattacc tgggctggaa cctgatcaat acggccgcca
     4081 atttccttag cgacgacatc gctctgcaaa atttcgactt ctacggccgc accctttcgg
     4141 gcagcgaaga aatgagggcc cgttggaaaa gagctttggg agcaataaac ggaaccttgg
     4201 gagaagctct gggacaggtc tacgttgaaa aacatttccc gccccgggcc aaagaacgca
     4261 tgctcaaact ggtcgacaat ctccaactca gcctgggtga gagaatcagc tctctggaat
     4321 ggatgagcga agaaaccaaa caaaaagcct tggaaaaact gggcaccttc catgttaaaa
     4381 tcggctatcc ggacaaatgg aaagattaca gcagcctcga aatcaaagag gattcttatt
     4441 gggagaacat ttgcagggct tcgctctttt cgttcaacga aggcatggat aaagtgggca
     4501 aacccgtcga caaagaggaa tggcttatga atcctcaaac agtgaacgcc tactacaatc
     4561 ccaccaccaa cgaaatctgc ttccctgccg gcattctgca acctcctttc ttcaatatgg
     4621 acgccgacga tgccgtcaat tacggagcta tcggtgtggt gataggccac gaaatgaccc
     4681 acggtttcga cgaccagggc agtcaatacg ataaagacgg caatttagcc aattggtgga
     4741 gtcccgaaga ttccgaaaag tttgcagagc gtacggccgt tctcgaagaa tatttcaaca
     4801 agatcgaagt agccccgggc gtttttgcta acggcaaatt cactttgggc gaaaacattg
     4861 ccgaccatgg cggcctgcag atttccttcc atgcctacac caaaacagac gaatacaagc
     4921 agaatcaaag catcgacgga ttgacaccgg cacagcgctt ctttattgcc tacgctcatg
     4981 tttgggccaa caacatcagg gaacaggaaa ttttacgcag aaccgccgaa gatccgcatt
     5041 ccctgggcta ttggagggtg aatggagctt tgcctcatat agacgctttt gttgaggctt
     5101 tcaacctgaa agagggcgat gccatgtttt tacccaaaga acaacgtgct tccatctggt
     5161 aatttatgaa gcaagtgttg caagaagtac aaaagatcaa cataaaagac tacgattacg
     5221 gcctgccgga cgaacgcatc gcgagatatc cggcagtgcc ccgtgattcg tccagactct
     5281 tgatttacga cggaaaaaac ctgtcacaag atatttttcg taagattgcc gaccatctgc
     5341 cctctccggc tctgatggtg tttaacaaca caaaagtgat ccatgcccgt ctgctgtttc
     5401 acaaaaaaag cggggccggt atcgaaatct tttgtctgga accggcccaa ccggccgatt
     5461 acatccaaag ctttcagagc ctgggcagtt gcgaatggta ttgtctgata ggcaattcca
     5521 aaaaatggaa ggagggcagc ctgaataaag aaatccgggc cgggggctta actattcaac
     5581 ttcaagccac ccgcaccggc caaaacggac ggctgcaagg catccgcttc gattggcaat
     5641 gccctcaaaa aactcttcag ttttcggagg tattggagct ggccggcatt attcccattc
     5701 ctccttacct taaaagagaa tcggaaagca gcgatgaaaa agactatcaa accgtctatt
     5761 ccaaaatcaa aggttcggtg gctgccccca cggccggcct gcatttttcg gaagaagaac
     5821 tcaaaaatct tgaccggcaa catattctgc gccgggagct cactttacat gtgagtgcct
     5881 ctacctttca atccgtcaag tccgatcgtt tgcgtgaaca ccccatgcat ctggaatttt
     5941 tttcggtgga gcgcacattg atcgaagaca ttgttcggaa caaaaatccg ctgattgccg
     6001 tcggcactac ttcggtacgt actttggaaa gcctttactg gtttggattg cagttattgg
     6061 agaacaaagc ggccgcaccg gaagacccgg ttgttgacca atggtttccc tacctcgatc
     6121 accaaactta taccacagag gaagctctta ctgctcttct cgactacctc gaccgcaata
     6181 agctggatcg tttttgcgct tctacaaggt tgctcatagc ccccggctat cactttaggc
     6241 tggtagatgc catgctgacc aattttcacc aacctcaaag taccctgcta ctgctggtgg
     6301 ccgcctttgt gggtcgggac tggcagcgta tttatcgcta tgcgctggac gagggcttcc
     6361 gttttctgag ttatggagac agttccttgc tcttccccgg aaatcagcag acgaaaacct
     6421 aaaaataaat tttcttcata agcctcttac ctgcaaaaag aggataacaa taagaaaccc
     6481 ggctttacgt ttaattaggt atgcgtcact tcatcggata cattttagtt ttatttctgt
     6541 cggccctggc ttcctgctca gtgagcaagg atatccgtaa ggcggataaa cactacgact
     6601 acggcgaata caacgctgct gctgtcattt ataccaaagc ctaccggcgc attcctgccg
     6661 tcgaaaaaaa tgtaagagca gaagtcgctt accgtcaagg cgaatgttat cgttttatca
     6721 atcaatctgt taaagccgag aatgcctata tgaaagccat tcgttacggt ctgccgaacg
     6781 atagcgtcct attgcattat gccgaagttt tgcgcaaaaa tgccaagtac gaagccgccg
     6841 aaattcaata ccggcaattt ctcgaaaatc atcccggcga cttacaggct agctatggac
     6901 ttgaatcctg cctgctcgcc cgtgagtgga aaaacaaacc aaaaaaatac gctgtcaaca
     6961 gagtgcagga gctttatctg aaaaggggta atttttcacc cttgctggtc ccggcagaat
     7021 ataaaaccct gctttttact tcgtcttcca tacaaaaaga aggcaaaaaa ccgagtaaga
     7081 tcaccggcct gcccgacaac gattttttta tcaccaggct cgaccaaaac gaaaaatggg
     7141 aaaaacctct tcccatagaa ggggccatca attccgagtt tgacgagggg gtgggctgtt
     7201 ttgatccgga cgggaaagtg ctctatttta cccgttgtgt caataaatca gactcgactc
     7261 tttcaggttc cagcgccgag atttaccgct cggttcgttc gggggaacaa tggagtgaac
     7321 ccgaaaaatt aaacattttc agagacacca acgccgtctt tgctcatccg gctatttctc
     7381 ccgacggatt gtacttgtat tatgtttccg acatgaaggg aggttatgga ggcaaagaca
     7441 tctggcgttc tgcgttttac gggaaatctt ttggcccgcc cgaaaatctg ggcccacaaa
     7501 taaatacccc gggcgatgag atgtttccaa ccatcaggac agacggcagc ctgtatttct
     7561 cgtccaatac cttgccggga ctgggtggat tggatatctt caaagctgtt tacaacgagg
     7621 aagacagtag ctggacggta gaaaatgtat acgacctcaa ctccagtgcc gacgatttcg
     7681 gcattacgtt tttcggcaaa aaggaaaaag gcttattctc ctccaaccgt aacgaaccca
     7741 ggggctggga caaaatctgg tctttcggcg aacccgaaca attaacccaa atcagcggct
     7801 gggtcaccga ccgttttgga gagcccattc ccgaagccac catccgaata gtaaacgaca
     7861 aaggcaccaa taccaaggtg gtctcgcgca gagacggcag ctacagttac caggtggaaa
     7921 aaaacgccga atacgttatg ctgggcacct gcaggtcata tctgaattac tccaatcaat
     7981 ttgtaaccac agacaaggac agcaattatg tggttaattt cagcctgact tctttgtatt
     8041 tgcctgtgcg tatcgaaaat atattttttg aattcgatcg ctgggaactc aggcccgaat
     8101 cgggaccggc cctgcaggaa ctccttaaac tgttgctcga caatccgcat ataaccatcg
     8161 aaataggagc ccacaccgat cgcaagggcg acgaggaata caacctgaaa ctttccgaaa
     8221 aaagggcgga atccgttgta aattatctga taaaatacgg tatagacaaa gagcgcctca
     8281 gcgctcaggg ctatggaaaa agcaagcctg ccaaagtcga caaatatatg gaaaccaatt
     8341 atccttactt aaaagaaggc agttttttgg acgaatcttt tgtagaaacg ttatctttgc
     8401 aacaacaaga aatagccgac cagatcaacc ggcgatgtga atttaaagtc ttgaaaacaa
     8461 cctataaatt attctgatga aagcctattt agacctgttg cagcgcgtac tcgacgaagg
     8521 agtagaaaaa aaagaccgca ccggaaccgg agccattagt atatttggac atcaaatgcg
     8581 ttttaagctc agcgaaggct ttcctgtgat caccaccaaa aaactgcacc ttaagtccat
     8641 catttatgaa ttgctctggt ttttgaaagg cgacaccaac gtccgctatt tacaggaaaa
     8701 cggagtgcgc atctggaatg aatgggccga cgaagacggt aatctgggac atatctacgg
     8761 ctaccagtgg cgctcctggc ccgattaccg gggcggtcac atagatcaga tcagcaaggc
     8821 cgttgatgag ataaaaaaca atcccgattc gcgtcgcatt atcgtaaatg cctggaatgt
     8881 ggccgacttg cccaatatga acctgccgcc ctgccatatg tttttccagt tttacgttgc
     8941 caatggacgg ctcagcttgc aaatgtacca gcgcagtgcg gacatttttt tgggggtacc
     9001 cttcaatatt gcctcttatg ccttactcct caaaatgatg gcccaggttt gcggactgga
     9061 agccggcgat tttgtgcata ctttgggaga cgctcatatc tatttaaacc acctggagca
     9121 ggtcaggctt cagctctccc gcgaccccag gcctttacct gaaatgctga tcaatcctga
     9181 agtcaaagat atcttctcgt ttaaatacga agatttccaa ttgattaatt acgacccgca
     9241 tccccatatc aaaggagaag tttcagttta agcaaaaatt ctgtactttt gcttgtttat
     9301 ttatactccg tattatggaa accgctatta ttgccgccat tgccaatgac tatgctttag
     9361 gactggacaa caagctttta tgccacttgc ccgccgacct gaagcatttt aaggaattga
     9421 cctccggaca tacggttgtt atgggacgca atactttttt ttccctaccc aaaggcgctt
     9481 tgcccaatcg cagaaatatt gtactcagtc ccgatgaaga aacatttacg ggctgtgaaa
     9541 cagcgcactc tttcccggag gtttttgagc tctgtcaaaa agatgaaaaa gtttttttta
     9601 tcggaggagc catggtctat agccaggcta tggaatgggt ggattctctg tacattacct
     9661 ggatacacgc ctcgttcgag gccgatgtct ttttccccga aatcaatcag gatatatggg
     9721 aagaagccga acgggaagat catttcgatg aagaacctta tccttattct tttgtacatt
     9781 atgtcagaaa aaaagattaa tacttgctcc ggctttctga taggccttct tttcgtgctt
     9841 ttctccggat tgtcgtttgc acaaacagct ccctatgccg agattgcctt cgaaaaggca
     9901 atacacgatt acggcacgat caaagaatcg ggttacgctc actgtgagtt tgaattcagc
     9961 aacacaggca acgccccttt ggaaattcgc cgtgtaactg cctcctgtgg ttgtaccact
    10021 cccgattatc ccaaagctcc catcgctccg ggagcgaaag gcataattaa agtagcttac
    10081 aacactacag gccgtccggg ccctttcaac aaaagcatca ccgtttattc caatgccacg
    10141 gccaatccgg taataatgct cagcatcagg ggtacggtcg aatcaaacaa caacgatgca
    10201 gaacgtttgt ttcccaaaac gatgggcggc ctcaggctaa aaagaaccat gattcctttt
    10261 ggagatgtat tgataggaag catcaaaacg gaaaccattc ctgtgtataa tcaaagcgaa
    10321 gcccccatag agttgagttt taacaaagtg cccgctcata tgagggttat gatttccaac
    10381 tcacggctgg agcacggaaa caccggaata attaccatca attatatccc cgaagaagcc
    10441 agggattatg gtcaaagaca agatttcttt tatatagtca ccgatcccgc agaaaaggaa
    10501 aatcctgcca acataataat tgtcagcgcc aatattaagg aggacttttc aagaacaggc
    10561 cccttggata aggtgcccag agcatctttc agccaaacgg gcatagattt gggaaaggtg
    10621 tcaggcacgg aaaaaaaaac cgctgaactg gttttgaaaa acccgggcga aaagccgctc
    10681 ataatcagaa aagctactgc cacttgcaat tgcatagagt taaaagccga ccggaaagag
    10741 attgctcccg gcaaggaaag ccgcgtccgc attgttttcg aagcggggaa attgatcggg
    10801 aacctgcact ataccctgga atttattacc aacgacccca ggcagccgat aactaaaatc
    10861 cctctcactg tcaacgtcat cgctaaatga aacaccccga aaacgacgct cggtaccggg
    10921 gattgacagt aaacaaaggg atagaccagc cccctatcat caatccgtat tcaaagaaaa
    10981 ggagtccgag aaaacaatac tccgtcgaag aatacgtaca gggaatactg gacggcaaca
    11041 acgtcatcct gagtcaggcc gtaacgctta tcgaaagttt gagacccgat catcaggaaa
    11101 cggctcacga aataattgag cgttgcctac cctacgccgg ccgttctgta cgctggggta
    11161 ttaccggagt tcccggggca ggcaaaagca cttctatcga ggcattcgga atggagctga
    11221 tccggcaggg acggaaatta gccgtgctgg ccatagatcc cagcagtgaa cgcagcaaag
    11281 ggagcattct gggagataaa accagaatgg aagccctgtc ggtggaaccc aaagcttttg
    11341 taaggccttc gcccacgggg ggatctctgg gcggtgtagc ccgcaaaacc agggaaagta
    11401 ttgtactttg cgaagcggcg ggcttcgaca ctatttttgt agaaaccgtg ggagtcggac
    11461 aatcggaaac agcggtacat tccatggtcg atttctttct gctgattcaa attgccggag
    11521 ccggcgacga attgcagggc atcaaacggg gcattatgga aatgtccgac ggtattgtga
    11581 tcaacaaagc cgacggcaac aacatcgacc gggctaattt ggcggccgcc cagtaccgta
    11641 gtgcgctaaa gctttttccc atgcccgact cgggtgtgcg cccgcaggtg ttatgctatt
    11701 cggctcagca attgtcgggt gttgccgaag ttattgaaat ggtaaacagg catatcgaac
    11761 tcttcaaaac caatggcagt tttgagcaaa gacgcactca gcaaagtatt tactggatgc
    11821 ggcaaagcat cgacgaatcg ctcagaaacc acttctatca caaaccggaa atggctgcaa
    11881 tgctcaaaga gaaagaacag gaagtcttga ataacaaaaa aactcccttc agcgctgccc
    11941 gcgaactcct cgacttttac tataactcct gagtactttc gcccgaaact tgaagcttct
    12001 tttcaatttc ccgacggact tcttccatca gccagcgtgg agtagaggta gctccgctga
    12061 ttcctatacg ctgaaatcct gccacatcaa tcgtacgaac ctcaccggga cttgagatga
    12121 aatgggtgtt gtcattcacc tccttgcaag ccttatagag tgattttccg ttggagcttt
    12181 ttgcgtcgct tacaaaaaaa accagatcgt tggccgctgc aaaatctctg acttgaagca
    12241 cccggccgga catatgccgg caaatggtgt cgaaatgttg aaaaccgacg gcagggttta
    12301 tttcgttcgt tattgcctca acaagctgac gatactcctc gagcgatttg gtggtttgcg
    12361 aaaacatgat gacgggcctg tcgagatcga cctttaccaa gtcggctaag tgttccacca
    12421 ctatggccgt attttgggtt tggcccacca aaccgtttac ttcggcatga ccgggtttgc
    12481 cgaaaattac gatttgcgtt ccgtcgtttt tgtgttcctg ataagcttta tgtactctcc
    12541 tttgcaaatt taagactacc gggcaggtag catcgaccaa ttccaagccc tgccgttcag
    12601 ccgttttata ggtagaaggt ggctcgccgt gagcgcgaat cagaattttt ttgtttttca
    12661 gaccggcaaa ctcatcgtaa tggacagtct tcatgccttt tttttccagg cgttttacct
    12721 ccatattatt gtgcacgata tcgcccaggc aataaagttc ttctttgctg tccaagtgct
    12781 cctcggcctt ggcaatggct ttgaccacac caaaacaata acccgaatgt ttatcaattt
    12841 cgattcttgc catacaccgc ttcattaaaa cgagcctcca accaggcatc ctgttcttct
    12901 atactcatcc gaccgttatc cagttcaatg gcatcgtcgg ctttgcgcaa agggctgatt
    12961 gttcggctta ggtccatttg gtcgcgcatt tctatgttat ggagaatttc ctcgtaactg
    13021 gcttcgatac ccttctcttt caattccaaa taacgtcttt tggcacgaac atcagcagat
    13081 gccgtaagaa aaatcttcaa ttctgcttcg ggaaaaacag ccgttccaat gtcgcgcccg
    13141 tccattacta cgcccttgtt ttcagccaat gaacgctgaa tatcaactag ataagatcta
    13201 acctcgggca cagcactgac caggctgacg actccggaaa cttccatacc cctgatttcg
    13261 ttttcgacct cctcgccatt aagacaaata cggggaaggc ctttgtccgt agtgctaaaa
    13321 ctaatggtga tgtctttcag tccttcaacc aaagcctctt ggtccagaca cgtgcccttg
    13381 atccagccct ttctgaggca atacaaggcc acagctctgt acatagctcc gctgtcgatg
    13441 tagagatagt tttgtttctg cgccaattgt ctggccatag aacttttacc gctcgaagaa
    13501 tgaccatcga tggccacaat aattcgtttt tccattgctt aaaagatacc cagattggta
    13561 gaaagggtca tttgtaaagt attgccggca atatggtatt gagcaaaaga agctcctatg
    13621 cgcagttttc gcacagacat ggaaaatcct gcagtaaaac cggtcaaaat agtccgttga
    13681 gccagcgata attcagattt acgccggacg ttgtatccca ggctgagttg aaaattatcg
    13741 gagggcacaa attccaaacc tatcaacaag tgtttgaaag cctttccaac aaaattatcg
    13801 ttgtccacta cccgtccggt agttccgtct aagtaagggt aggaccaggc gttaaacccc
    13861 caggctgtaa gggtaaaccg gaaaggagca tgcaacaagc ccttggtaaa acccaactgg
    13921 acatcccagg gcaacgcttc gtagctctca tcgtaaggct tgaactgcga tccaaggttt
    13981 ttgagcgtaa aacccgccca gattttttgt tccggattgg cgtattgaac acccagatct
    14041 acagccacac ccaaagaagt ataatgttcc agcaccgaat aaaccagatg catgccggcg
    14101 cctgccctcc aacgaggaga aagcataaaa gcgtagcctc cgctcatggc catgtctttg
    14161 acaaaaaatt caccctcggg caggttgtct ccggcactaa ttatgcgccc gtaatccaca
    14221 taacgtaaag ctgccgacca aatacttctt tcattaatct tgcgagcata ggccgctgtc
    14281 ccaaggtgaa tatcggctac gtaattcata tagcccaaag ccaattggtt gtgcatttcg
    14341 ccggaaaggg ctgccggatt gtgtaagctt aaattcaaat cgttttcggg agaagaaagc
    14401 actgtgcctc ccaagcctgc agcataagaa gacacgggca aggtcaggaa gcgataaacc
    14461 ccttccccgg cccgtattct taacagaacg accacaagca ttaaaagcac aagcaaccca
    14521 cgtcgcataa ttcccggttt tagaaccatc aaaggtagta aatttccata gtaacgcagc
    14581 ctcgaatttt atttatgaaa acaacggaag agattttttt tatttgcctt ctctttttta
    14641 ttaaaaaaaa tgtatctttg tcggtcaaca gaatccatat ctttttacat caaatgccat
    14701 gcaagtaaaa aaatcagcaa gagccgatct tgacaaaaac tccaccaaca attttttgat
    14761 ggggctggtc attgctttgg gcttcctttt catcggattc gagtactcca acaccgaagt
    14821 tacggttcag gacatttaca ctcagtctgg tgaaatcgaa gaggagataa tgatagaaat
    14881 taccaggcag tacatccccc cgcctccccc tccacctcaa actgtacact ctgaccttat
    14941 tcagattgtt gaggacaata ctctggaaga agaagttgaa tgggcttcca tagaagattt
    15001 tcctgacgag gctattgtta tgacatacgg cagcggtgat attacagaag aatacgaccc
    15061 cgatggcgaa gttttcatgt ttgttgaaga aatgcccggc ttccccggag gagaatcagc
    15121 actgatgaaa ttcatcaacg ataacttgag atacccgcag cttgcgttgg aaaacggcat
    15181 atccggccgt gttatttgta cctttgttat cgatggacgc ggaaaggttt ctgatattca
    15241 ggtaatccgc tcggttgacc ccaacctcga ccgcgaagcc acaagggtaa tcagcatgat
    15301 gcccgactgg aaacccggaa aacaaagggg aaaacccgta cgtgtacgtt atactctgcc
    15361 tataatcttc aaattacagt agtagattac acacctatcg aagataaata acacacaaaa
    15421 tacataggtt tccctttgat gtattttgtg tgttattttt tactttataa aaacatgaaa
    15481 agtattcagg aaatcagaac attaatacaa aatggtatcc gggaaatcga ttttcctgat
    15541 cggccttcgg gtttatacga accgctgaaa tacgtccttt cgctgggcgg gaaacgtctg
    15601 aggcccctgc tttgcctctc ggcttgcaac ctgtacaacg aagaggccct ttcaaaagcg
    15661 ctacccttgg cacttggcgt ggaacttttc cataacttta cgctgataca cgacgactta
    15721 atggaccgct ccctagtgag acgaaacaaa gctaccgttc acacaaaatg ggacgaaaaa
    15781 acggctgtcc tttcgggtga cgccctgcaa attatggcct ttcaacaaat agcattggct
    15841 ccagaagaca aactcaaaga ttgcctggac ctgtttaatc aaacagccag agaagtgtgc
    15901 gaaggtcagc agctggacat gaactttgaa atgctcgaca atgtccgtaa agaggattac
    15961 atcgaaatgg ttcggctcaa aacggctgtt ctattggggt gtagcctaaa actgggcgcc
    16021 attataggag gagcccccga caacgaggct gattttcttt acgacttcgg tattcacctc
    16081 ggcatagcct ttcaaattag agacgactta ctggatgtgt acggagatcc ggatattttc
    16141 ggtaaagcca taggcggtga cattgtaaat aataaaaaaa cttttctctg gatacattcc
    16201 attgagacag ccgacaatca tacacgcagc ctctttttga attgcatgaa gagcaactac
    16261 atggacaggg aggaaaaaat aaaaatggta acccgttttt acaaccggct gggggccaga
    16321 gaaagttgtg aacaacagat gctggatcac cagcagtctg cccttaaagc acttgagggc
    16381 ctggatctcg acaaaaagcg tttagttaca ttgagcgaac tggccggaaa actgacctac
    16441 cgccaagtat aataaatttt tgtccgatta tgccatacag aagattgccc aacaccgaca
    16501 gagcacgtat acgcgctctg aatgctgcca ctaaacaagg agatttagta ggctttcttg
    16561 acttggctta ttcatacaaa ctcaaaaacg acgccctgtt gattttgtct cagattgaac
    16621 aggcttatca cgaatacaac atcaatctgg agaggcaaac caccagtagc aaatcctatc
    16681 tggctcacct taaaacggcc agattataca tttcccattt tatacaggta ctcaatctga
    16741 gcgtcttgcg caacgaaata aaaaaagagc acaaaaaact ttatcggctg gatgtcaacc
    16801 gacaaagtat tcctgattta agtacg
//
LOCUS       DAOSGL010000009        16820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_2780_length_16820_cov_5.075753, whole genome shotgun sequence.
ACCESSION   DAOSGL010000009 DAOSGL010000000
VERSION     DAOSGL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2780_length_16820_cov_5.075753"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKY68_00670"
     CDS             <1..180
                     /locus_tag="PKY68_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23586.1"
                     /translation="SSRKLGLSKLNMAGMGSKLMRTIMRNKQVIWQTAIQLVPTRLYQ
                     RINNSGRLANRSTDV"
     gene            complement(166..1668)
                     /locus_tag="PKY68_00675"
     CDS             complement(166..1668)
                     /locus_tag="PKY68_00675"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOH23587.1"
                     /translation="MKDSGISMNPNELVQFLNKPSQDFTRKDIMRFCEERQIKMLNFR
                     YVAEDGRLKTLNFVINSRDYLETILTRGERVDGSSLFSFIESGSSDLYVVPRYRTAFV
                     NPFAEVPTLDIFCSFYNNHGRPLESDPYTILHRADQLFTKETGSRFMALGELEYYVIA
                     PAGGLYPGVDQKGYHASEPFAKYEFIRNEALQMIARAGGNIKYGHSEVGCFTQGESYY
                     EQHEIEFLPCTVEKAAEQLVLAKWILRMLGNKYGVGISYAPKITEGKAGSGMHFHMML
                     EKDGKNLVAGKGGLSDVARKMIAGILDLSDAITAFGNTIPTSYFRLVPNQEAPTRICW
                     GDRNRSVVVRVPLGWTGAEKMIFDANPLESEPLPASESRQTFEYRVSDGSADIFLTLA
                     ALIVASLHGIRRPDALELAEQLYVDVNIFKPEHREKMQSLEQLPLCCADSAEALKRKR
                     AVFEENNIFPAGLIDSQIKKLQAYNDKGLSEKLFGKQDVLKLVEQFLHIG"
     gene            complement(1747..3219)
                     /locus_tag="PKY68_00680"
     CDS             complement(1747..3219)
                     /locus_tag="PKY68_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOH23588.1"
                     /translation="MYKILLRENSQTRVIQTQGQQSLLEILRNEDYSIYAPCGGKGRC
                     GKCLVDIKGEGKVLACNYYPAGDIEVILPAKEEAQILATQTAYLEDFPPAHSRQLLHP
                     DAYGAAIDLGTTTIVCYFVHLASGRIVKISSWMNPQTAYGADVISRISYCQENPGGLK
                     LLQKILTESINKEFNSFLSEMKAGKQQIQETVIAGNTTMLHILLGYNPEPLALAPFTP
                     AFTAQQVLGAENSSLLLHPQGRLITLPCLSAYVGSDIVAGLAALKAPQKNYLFLDIGT
                     NGEMALVKGEEIFTCATAAGPAFEGAGLSSGMPALKGAIAAFESPSNYKVIGDVRPVG
                     ICGSGVIDSVAWLRRNHLMDESGLIQQDFEIYPGIQITQADIREIQLAKSAIYSGIKI
                     LLKRAGLNFQELEALFLAGGLGNYIHIPSAVEIGLLPAELKDKTFAVGNSAGIGALQF
                     LRSEVFANKVDRVIQQSQYIELSDDEDFALEFAMNMDFLK"
     gene            complement(3212..4129)
                     /locus_tag="PKY68_00685"
     CDS             complement(3212..4129)
                     /locus_tag="PKY68_00685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOH23589.1"
                     /translation="MTKIDRSKMGLRLIVLIATVIVALFLSSLLLNLFLLLSRGLSFG
                     QLGSELGAPMSNPSFLLLMQGLQTLLVFILPPFLLSYWYGEKNSCFLKLVKAKPVDVL
                     LAMFSILAAVPLVNFLVSWNQDIQFPSFLEGVEKWMRVAEDNAQRVTELMLQGESWSD
                     LLWQLLVVAVMAGFGEELLFRGTLQSIISSKGRIQLAIWITAFVFSLIHMQFYGFIPR
                     LLLGAWFGYLLLWSGSIWVPVAAHFANNAISVVFHYAEKNNYLTSDPELLGVGENTWQ
                     LIPSILLLTACGLFFYLRYRKSTLKKQHV"
     gene            4294..5652
                     /gene="tig"
                     /locus_tag="PKY68_00690"
     CDS             4294..5652
                     /gene="tig"
                     /locus_tag="PKY68_00690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOH23590.1"
                     /translation="MNVEKQLIDPVNASIKINLVKADYQEKVDKTLKTYRQKANVPGF
                     RPGMVPLGLIQKMYGKAVLAEEINKIVLEALYNYIKENNLNILGEPLPGKQQTPPDFN
                     AGEDFEFIFDIALAPELDFTISKKDSIPYYKIEVSDDMLEKQIKSLASRGGRYEKVEE
                     AQEGDTIKGTLTEIPEEGQKDSEDLVKVEEAIILPAYFKNEEEKAKMKGVRPGDKIRF
                     NPYKAAEGHETELASMLHLPKEKAVEIKSDFEFEVKEISRFKEAELNQELFDQIYGKD
                     VITSEEAFRAKVREEIEARFAPESDYRFMVDAEKQLVKKLKGVEFPVEFLKRWLLAAD
                     EKRNPATIDQEMPKLIEELKWHLMKEEIIKRNNIQVSEADVLETAKKATRAQFAQYGM
                     MSVPDDLLNNYAAEMLKKPETARNLSDQTNSAKVADFLKSTVKLNEKTVSAEEFNKLY
                     EN"
     gene            5658..6323
                     /gene="clpP"
                     /locus_tag="PKY68_00695"
     CDS             5658..6323
                     /gene="clpP"
                     /locus_tag="PKY68_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOH23591.1"
                     /translation="MNDFRKYATRHLGMDGMALDQYSKITSSYISPTIIEERQLNVAQ
                     MDVFSRLMMDRIIFLGTQVDDYTANVVQAQLLYLDSAEPGKDISLYLNSPGGTVYAGL
                     GIYDTMQFISSDVSTICTGVAASMAAVLLVAGTKGKRFALKHSRVMIHQPMGGVQGQA
                     SDMEITVREIQKIKKELYSIISSHSGQTIEQIEKDSDRDYWMTAAEAKDYGMIDEVML
                     KSK"
     gene            6326..7561
                     /gene="clpX"
                     /locus_tag="PKY68_00700"
     CDS             6326..7561
                     /gene="clpX"
                     /locus_tag="PKY68_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOH23592.1"
                     /translation="MAKTSTDKCSFCGRSRNEVELLISGISAHICEECAERAHSISKE
                     IIGSSKNKQFELKAEDLPKPKEIKAFLDLYVIGQEEAKKFLSVSVYNHYKRLMQTKKD
                     DEVEIEKSNIIMVGATGTGKTLLAKTIAKKLKVPFTIVDATVLTEAGYVGEDIESLLT
                     RLLQVADYDVAAAEKGIIFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSVVN
                     VPPQGGRKHPEQKMIPVNTQHILFICGGAFDGIEKKIAARLNTQVVGFGASKQVARID
                     RENLLQYIAPQDLKAFGMIPEIIGRLPVLTYLEALDRKALRAILTEPKNSIVKQYIKL
                     FEMDGVTLSFDDDVLEYIVDKAIEFKLGARGLRSIAETIMMDMMYETPSGRRKNIRID
                     LKYARSKIEAANQSRLREQ"
     gene            7657..9837
                     /gene="recQ"
                     /locus_tag="PKY68_00705"
     CDS             7657..9837
                     /gene="recQ"
                     /locus_tag="PKY68_00705"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOH23593.1"
                     /translation="MAKSNPLAESLKLHFGFDTFKGNQEAIIRNIMEGKDTFVLMPTG
                     GGKSLCYQLPSLLLNGTAIVISPLIALMKNQVDAMRNFSDEDGVAHFINSSLNKAAID
                     QVRNDITSGKTKLLYVAPESLTKEENIDFLKQIPISLFAVDEAHCISEWGHDFRPEYR
                     RIRPLIDEIGRAPIVALTATATPKVQHDIQKNLGMMDATVFKSSFNRPNLFYEVRAKT
                     SSIDKEIIKYIKANPGKSGIIYCLSRKKVEKLAETLQVNNIKALPYHAGMDSQTRTAN
                     QDAFLMERVDVIVATIAFGMGIDKSDVRFVIHYDIPKSLEGYYQETGRAGRDDGEGYC
                     LAFYSNKDLQKLDKFMQGKPVAEQEIGKELLLETAAYAESSVCRRKSLLHYFGETYDK
                     ENCGNCDNCINPKKQVEAQELLSMVLETVVAVKEKFKTDHIIDVLSGNETSDVVSYKH
                     NELELFGMGAEEDEKIWNAVIRQALIDGYLDKEIENYGLLKITDSGRNFLKKPRSFKV
                     TEQLDYEEMEEDMQMHGSATSAVDPIMFSMLKNLRKKISKQLNLPPYVIFHDPSLEAM
                     ATTYPITMEELQNIPGVGAGKAKRYGQEFLSLIKIHVEENEIERPEDLRVRTVANKSK
                     LKVSIIQAIDRKVALDDLANTKGLGFSELLDEIEAIVYSGTRINISYFLMEIMDEEHI
                     EEIFDYFKDSSSDSIQEAMDVLGGDYTEEEIRLVRIKFISEYGN"
     gene            9890..11812
                     /locus_tag="PKY68_00710"
     CDS             9890..11812
                     /locus_tag="PKY68_00710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH23594.1"
                     /translation="MRKILLACLLSVIFSNVRAQADDPVVMNINGEDILRSEFEYNWK
                     KNYNISSTDKKNLDEYLELFVNFKLKVAEAKAQGIDTTQAFITELRGYRSQLTAPYLT
                     DKQTEEKLIDEAYAFTSQYVDLSHILIPLDRNAAPQDTLTVYNQLMELRERILKGEDF
                     GRLAAEYSQDNSSRDGGRIGTFLCSRLIYPIAKTAFYSQPGEIGLPVRTAFGYHLIKL
                     NAKHQAPGQYISGHILQSAPSGAPKEVVDSAKQHITEIWGLLESGQDFAQLADTRNDD
                     RYVVGKGGKYQELRPGSLPYEYEQQVFDLKDGEYSQPFKTEYGWHIVKRFEITPLPEK
                     GEILEELKSTLQRDERAQAGRKALVNQLKKDYNYQPDTKALAQFEQTLAGLENPDSTL
                     RAEAARLPVLASFEQNSITPTDFAEYYLQRKSLITSVPTAFEDYVEQRILAYEDSRLE
                     KKYPDFGNLMREYHDGILLFEISNQKVWEKAVEDQEGLAAYFKANKADYQWDKPRFKG
                     ALIHCADETTAKKAKKMAGKLKDDSLAFELKRSFNNDSTTLIKVEQGIYVMGEYAPVD
                     KLVFKSGDWNPDKSFPIVFTKGKVLKKGPEEYSDVRGAVIGDYQNYLEKEWIKELKQK
                     YPVSIFEEHLQILKAE"
     gene            11863..12762
                     /locus_tag="PKY68_00715"
     CDS             11863..12762
                     /locus_tag="PKY68_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH23595.1"
                     /translation="MKHLLWLLLLMPAWLQTCLGKETESPRVAVVRVSDATLYLDEIQ
                     NALPTGLNSVDSIRFVNSYTENWVKETLLYQAASKNLGQSPEINRMVENYRRSLIIYQ
                     YQQQALNEKMESEVTEADMAEYYEKNANRFISNKNLIKGLFIKVPLSAPGLDKLKIWY
                     KKNDTESLDNIEKYSYQNASIYEYFHETWTSLDDVMDQIPANIADQEKFLRNNKFLEV
                     EDGNYCYLLYISDYITTGSPQPLDYVSAQIRNILVNSRKTEFIHQFENDMLEQAKRKG
                     AIKYFSIPVVDTASAQDLTIDPQ"
     gene            12759..14108
                     /locus_tag="PKY68_00720"
     CDS             12759..14108
                     /locus_tag="PKY68_00720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH23596.1"
                     /translation="MKQFIYLIALILAFALQSLQAQNNIVDEIVWIVGDEAILKSEVE
                     AYRLQALQEGQKFSGDPYCVIPEQLAVQKLFLHQAELDSIIVTDNDVITNVDRNINRM
                     ITVLGSKEKMEEYFGKPISKIREEQREYARNEQTIHLMKQSLLKGLSVTPAEVRAYYK
                     SLPSDSIAIIPAEIEVEIITVEPRIPEAEIESIKERLREFTERVNNGERFSTLAVLYS
                     QHEATAKFGGETGFYGKGELMPEFAAVAFSLTDPTKVSRIVETDDGFHIIQLIEKRGD
                     RVNCRHILLKPEVSATERNEAMVRLDSIADFIRKGKLSFADAAMFYSQDKNTRKNGGL
                     MFNPNTGTAHFQLEELPQEVSKVVYSMNIGEISAPFSMRNPDNDREICAIVRVKNRTK
                     AHKATVEEDFVKLKDIVLQNKQEKLIREWIVNKQKGIYVRIDERWRNCDFQYPGWIK"
     gene            14059..15744
                     /locus_tag="PKY68_00725"
     CDS             14059..15744
                     /locus_tag="PKY68_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOH23597.1"
                     /translation="MKDGGTAISNIRDGLSNMFNVADIKPALRHMSLGLLCLCSLVLQ
                     AQGNRIFLEHANSTNFDKNISPDYHFLIGDVQFRHDSARLYCDTAHYYRADNSLRAFG
                     NVRIEQGDTLFIFGKTLFYDGNKKLAQLRNNVRMVNKEVTLFTDHLDYDRVANIGYYY
                     RGGKIVDASNTLSSDYGRYNPDTKMAFFKKDVVLTHPQFLLKTDTLNYNTETGTASII
                     SRTEIEAENADIIAYRGWYNTKTDKSLLLDRSYIISEKRSLTADSILYEQSLDIGEAF
                     VNIELIDSSRNTTLTSDYAFYDQSNNYVLLTKKALLREYSGQDTFYLHADTLLGKQDS
                     IYDTFRAYYNVRSYRRDLQGLCDSAFYSTRDSVLQLFGNPILWADNQQISGQQILLYT
                     KNQQPDYMEVEGLALLASKEDSGLFNQSSGRKLTAYFLDNKVRRVVIEGNPESIYLPR
                     DEKQKILGMNRLENGSLYIFMDEEGKVERLKVSPQPKGKFYPLQLLTEEVKYLEHFSW
                     PIHLRPTDPADVFRLTDRPRAENMSTNIGGAAGEGANAPGRAALRTFKSKNSK"
     gene            15746..16045
                     /locus_tag="PKY68_00730"
     CDS             15746..16045
                     /locus_tag="PKY68_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23598.1"
                     /translation="MSFFKQRKPRKFDHQPIYYDAQKEARQERLQKAKVELGLMDEKE
                     LKKDYEKVIRGSFREAVPGGSRTKRKQDSNKTMRILVVLFIIILVVYFLASRYAG"
     gene            16035..>16820
                     /gene="mutL"
                     /locus_tag="PKY68_00735"
     CDS             16035..>16820
                     /gene="mutL"
                     /locus_tag="PKY68_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOH23599.1"
                     /translation="MLDKNLAGNTIRVLPESVANQIAAGEVIQRPASAVKELMENAID
                     AGASQIDVLVKDAGKSLIQVTDNGKGMSPQDAILAFERHATSKLSVATDLLKIRSMGF
                     RGEALASIASVAQVELQSKRPEDELGTKVSIAGSKIENQETVLCASGSRFSVRNLFYN
                     LPARRKFLGSNAKEWLHVRSEFIQIALAYPELQLSLVHNGESIFRLSPSGLRQRIVAI
                     YGEQLNKKLFPIEVETEIVRISGFVGSPDAARKRASEQFFFVNR"
BASE COUNT         5169 a         3862 c         3720 g         4069 t
ORIGIN      
        1 tccagccgca agctgggact ttccaagctg aacatggctg gcatgggcag caaactgatg
       61 cgaacgatta tgcgcaacaa acaagttata tggcagacag ccattcaact cgttccgaca
      121 aggctttatc aacgaatcaa caacagcggt cgtctcgcga acagatcaac cgatgtgtaa
      181 aaattgttcc accaatttca aaacatcctg tttgccgaac aatttctcgg acaagccttt
      241 gtcgttgtac gcctgtaatt ttttgatctg gctgtcgatg agaccggccg ggaagatgtt
      301 gttttcttcg aaaacggctc ttttccgctt taaagcttcg gcagaatcgg cacaacacag
      361 cggcaactgt tctaaagact gcatcttttc ccgatgttcc ggcttgaaaa tgtttacgtc
      421 cacataaagc tgctcggcca gctcaagagc atccggccgc ctgattccat gaagagaagc
      481 aacaatcaaa gcagccaggg tcaggaaaat atcggccgag ccgtcggaca ccctatactc
      541 aaaggtttgc ctggattccg aagcaggtag aggctccgat tccaagggat tggcatcaaa
      601 gatcattttt tcggctcctg tccagcccag aggcacgcgt accaccaccg agcgattgcg
      661 gtcgccccag caaatacggg taggtgcttc ttgattgggc accaatctga aataagaagt
      721 aggaatggtg tttccgaaag ctgtaatagc atcgctcaaa tccaaaatgc cggctatcat
      781 cttgcgggca acatcgctta atcctccctt gcctgcaaca agatttttac cgtctttttc
      841 gagcatcata tgaaaatgca ttccgctgcc ggctttgcct tcggtaatct tgggagcata
      901 actgatacct acgccgtatt tgttacccaa cattctcagt atccatttgg ccaacaccaa
      961 ttgttcagcg gctttttcaa cggtacaggg taaaaattcg atttcatgct gctcgtaata
     1021 gctttctccc tgagtaaaac aacccacctc ggaatgtccg tattttatgt tgccacccgc
     1081 cctggctatc atttgcaaag cctcgttacg aataaactcg tatttggcaa aaggttcgct
     1141 ggcgtgataa cctttttgat ctacaccggg atacaagcct ccggccgggg caatcacata
     1201 atattccaac tcgcccagtg ccataaagcg actgccggtt tctttggtaa acaattgatc
     1261 ggccctgtgt aaaatcgtat acgggtcgct ttcgagaggc ctaccgtggt tgttgtaaaa
     1321 agaacagaaa atatccagag tgggaacctc tgcaaaggga tttacaaaag ccgtgcgata
     1381 acggggaacc acataaagat cgctgctgcc cgattcgatg aaagaaaata aactgctgcc
     1441 gtctacccgt tctcccctgg tcagtatcgt ttcgaggtag tccctggaat tgattacaaa
     1501 attcaaggtt ttcaaacggc cgtcttcggc cacatagcga aaattcagca ttttaatctg
     1561 ccgttcttcg caaaaacgca taatatcctt gcgggtaaag tcttgtgaag gtttgtttaa
     1621 aaattgaacc agttcattag ggttcatcga gattccggaa tctttcatcg aacgagattg
     1681 aattaaaaag tgcaaaattc atcctcttgc gcaagaaaaa caaatcttgt ggctttattt
     1741 ttagaattat ttcaaaaaat ccatgttcat agcaaattct aatgcaaaat cttcgtcgtc
     1801 ggacaattct atgtattgcg attgttgaat aaccctgtcc actttgttgg caaacacttc
     1861 tgagcgaaga aactgcaagg cgccgatgcc ggcactgttg cctaccgcga aggttttatc
     1921 tttgagttcg gccgggagca agcctatttc aacggccgag ggtatatgaa tgtaattgcc
     1981 cagaccaccg gccagaaaca aagcttccaa ctcctgaaaa ttcaagccgg cacgctttaa
     2041 taaaatttta atgcctgaat aaatggccga tttagccagt tgaatttcgc ggatatcggc
     2101 ctgagttatc tgtatgccgg ggtaaatttc aaaatcctgc tgaatcaatc cgctttcgtc
     2161 cattaaatga ttacgccgca accaggcgac cgaatctatc actccggagc cgcagatgcc
     2221 tacgggccgg acgtctccaa tgactttgta atttgaaggg ctttcaaaag cggcaatggc
     2281 tcccttaagt gcgggcatcc cggagctcag tccggctcct tcgaaagcgg gaccggcggc
     2341 cgtagcacaa gtaaatattt cctccccttt gaccaaagcc atttctccgt tggttccgat
     2401 atcgagaaat aaataatttt tctgtggcgc tttcagcgcg gccaaaccgg ccactatatc
     2461 ggagccgacg taagccgaaa ggcagggcag ggtaatcaaa cggccttgcg gatgcaacag
     2521 taaagaagaa ttttcggccc ctagcacttg ttgagcggta aaagccggag taaaaggcgc
     2581 cagggccagg ggttcaggat tgtatcccag cagaatatgc agcatagtcg tattgccggc
     2641 aattactgtt tcctgaattt gctgcttgcc ggccttcatt tcggacagga agctattaaa
     2701 ttccttatta attgattccg tcaaaatttt ctgcagcaat ttgagccctc ccgggttttc
     2761 ctggcagtag ctgattctgg aaattacatc ggcaccgtag gctgtttgcg gattcatcca
     2821 ggaactgatc ttcacaattc gcccgctggc taaatgtacg aaataacata ctatagtggt
     2881 ggtacccaag tctatggccg ccccataagc gtcgggatgc aacaattgcc ggctgtgtgc
     2941 gggtggaaaa tcctcgagat aagctgtttg agtggcaagt atctgcgctt cttctttggc
     3001 aggcaaaatc acttcaatat caccggcagg gtaataattg caggccagca ctttcccctc
     3061 tcctttgata tcgaccaaac atttaccgca acggcctttc cctccacagg gagcatagat
     3121 ggaataatcc tcgttgcgga gaatttccag caggctctgt tggccctgag tttgaattac
     3181 ccttgtttga gagttttctc tcaacagaat tttatacatg ttgctttttt aatgtcgatt
     3241 ttctgtaacg caggtaaaaa aacaggccac atgccgtcaa cagcagtata gatggaataa
     3301 gttgccaggt attttctcct acacccaaca gctcggggtc tgatgtcagg taattgtttt
     3361 tttcggcgta atgaaacact acggaaatag cgttgttggc aaaatgagcc gccacaggaa
     3421 cccagatcga tccggaccat aaaagcaggt agccaaacca ggcccccaac aatagacgag
     3481 gtataaaacc gtaaaactgc atatgtatca gactgaagac gaaagcagtt atccagatgg
     3541 ccaactgaat tctgccctta gaagaaatga tactttgcaa agtccccctg aaaagtaatt
     3601 cttctccaaa acccgccata acagctacta ccagcaactg ccacaacaaa tcgctccagg
     3661 attctccttg cagcatcaat tccgtgacgc gttgtgcatt gtcttcggca accctcatcc
     3721 atttttctac accctctaaa aaggagggaa actgtatgtc ctgattccaa cttaccaaga
     3781 aattaaccag cggtacggca gctaaaatac tgaacatggc caataaaaca tcaacgggtt
     3841 tggctttgac caatttcaaa aaacagctgt ttttttcacc gtaccaataa cttaatagaa
     3901 aagggggaag aatgaacacc aaaagggttt gcaggccctg catcaacaaa agaaaagaag
     3961 ggttgctcat tggtgcgccc aactcagaac caagttgccc aaagctcaaa ccacggctga
     4021 gtagtaaaaa aaggttgagt agtaaggagc ttaaaaagag agcaactatc actgtggcta
     4081 tcaacactat aagccttaag cccattttgc ttctgtctat ctttgtcatt caatgagcat
     4141 tttttcgcaa acctaattca aagttttgtc aattcaaaga ttaagagtat ttttgcgtct
     4201 ccaaaaaaat gcctttaaga gcaacaagca gaaggacaaa gccctgcttt gaaaagcttg
     4261 ttcggtaaaa ggacaggttc aaacatcaac aacatgaatg tagaaaaaca actcattgat
     4321 ccggtcaatg caagtatcaa gatcaactta gtcaaagccg actatcagga aaaagtagat
     4381 aaaacactca aaacctatcg ccaaaaggcc aatgtgcccg gtttccgtcc gggaatggtg
     4441 cccttgggtc tgattcagaa aatgtacgga aaagccgttc tggcagaaga aatcaataaa
     4501 attgtattgg aagctttata caattacatc aaagaaaata atctgaacat cttgggcgaa
     4561 cccctacccg gcaaacaaca aacccctccg gatttcaatg ccggcgaaga ttttgaattt
     4621 atttttgata ttgccctggc tcccgaactt gattttacaa taagcaaaaa ggacagcatc
     4681 ccttactata aaatagaggt cagcgacgac atgctggaga aacagattaa atcgctggcc
     4741 agtcgcggcg gaaggtacga aaaagtggaa gaggcccagg aaggcgacac catcaaggga
     4801 acactgaccg agattcccga agaaggtcaa aaagactccg aagatcttgt aaaagtggaa
     4861 gaagctatta ttcttcctgc ctatttcaaa aatgaagaag aaaaggccaa aatgaaaggt
     4921 gttcgtcccg gcgacaaaat cagatttaat ccttataaag ccgccgaagg ccacgaaacg
     4981 gaattggctt ccatgctgca tttgcccaaa gaaaaagccg tggaaatcaa gtccgatttt
     5041 gaattcgaag taaaagaaat cagccgtttc aaagaagccg aactcaacca ggaactcttc
     5101 gaccagattt acggtaaaga cgttattacc tccgaagaag ccttccgtgc caaagtgagg
     5161 gaagaaatag aggcccgatt cgcacccgaa agcgactatc gttttatggt agatgccgaa
     5221 aaacaattgg taaagaaact caagggtgtg gaatttcctg ttgaattcct gaaacgctgg
     5281 ctcctggcag ctgacgaaaa acgcaatcca gcaaccatag atcaggaaat gcccaaactt
     5341 atcgaagaac tgaagtggca tctgatgaaa gaagaaatca tcaaacgcaa caatattcag
     5401 gtaagcgaag ccgatgtcct ggaaaccgcc aaaaaagcaa cccgggcaca atttgcccaa
     5461 tacggcatga tgtccgtccc ggatgatttg ttgaacaact acgctgccga aatgctcaaa
     5521 aaacccgaaa cggcaaggaa cctgagcgac cagaccaaca gtgccaaggt cgccgacttt
     5581 ttaaaatcta ctgtcaagtt gaacgaaaag actgtatcag ccgaagaatt caataaactt
     5641 tacgaaaatt gaaaaacatg aatgatttta gaaaatacgc cacccgccat ttaggaatgg
     5701 acggtatggc tttagaccaa tattcaaaaa ttaccagtag ctatatttct ccaaccatta
     5761 tcgaagagag acaattgaat gtagctcaaa tggatgtttt ttcgcgcctg atgatggacc
     5821 gcatcatttt cctgggcact caagtagacg attatacagc caacgtcgtg caagctcagc
     5881 tgctttacct ggattctgcc gaaccgggca aagacatttc tttgtacctg aattctccgg
     5941 ggggaactgt ttatgccggg ctgggtattt acgacaccat gcagtttatt tccagtgacg
     6001 tttctaccat ttgcacagga gtggcagcct cgatggccgc cgtacttttg gtagccggca
     6061 ccaaaggcaa acgctttgca cttaaacatt ctagggtaat gatacatcaa ccgatgggtg
     6121 gagttcaggg acaagcctcc gatatggaaa tcacggtaag agaaattcag aagattaaaa
     6181 aggaattgta ttctattatc tcatcgcatt ccggacaaac catcgaacaa atcgaaaaag
     6241 attccgatcg cgactattgg atgactgccg ccgaagctaa agactacgga atgattgacg
     6301 aggtgatgtt gaagagcaaa taataatggc taaaacatct acggataaat gcagtttttg
     6361 cggcagatca cgcaacgagg ttgaactttt gatttccggc atttcggctc atatttgtga
     6421 agaatgcgca gaaagagctc acagcataag caaagaaatt atcggctcat caaagaataa
     6481 gcagttcgag cttaaggctg aagatttgcc caaacccaaa gagatcaagg cttttttgga
     6541 tctatacgtt atcggccagg aagaagccaa aaaattcctt tccgtttcgg tttacaacca
     6601 ttataaaagg ttgatgcaga caaaaaagga cgatgaggta gagatagaga aatcaaacat
     6661 catcatggtg ggggccaccg gcacaggcaa aaccttgctg gcaaaaacca tagccaaaaa
     6721 actgaaagtg ccttttacta tagtggatgc cacggtgctt actgaagccg gttacgtggg
     6781 cgaagacatc gaaagcctgc tgacacgcct gcttcaggtg gccgattacg atgtggctgc
     6841 tgccgaaaaa ggcattattt ttatcgatga gatagacaag attgcccgca agggagataa
     6901 tccttccatc acacgtgacg tcagcggcga aggagtgcaa cagggcttgc tgaagctgct
     6961 cgaaggttct gtggtgaatg tcccgcctca gggagggcgt aaacatcccg aacaaaaaat
     7021 gattcccgtc aacactcaac atatattgtt tatctgcggc ggcgcttttg acggtataga
     7081 aaaaaagata gccgctcgct taaacaccca ggtggtcggt tttggagcct ccaagcaagt
     7141 tgcccggatt gatcgtgaaa acttactgca atacattgcg ccacaggatc tcaaggcctt
     7201 tggaatgatc ccggaaatca tcggcaggct gcctgtactt acctatctgg aagccctgga
     7261 tcgtaaggca ttacgagcca ttcttacaga accgaaaaat tccatcgtta agcagtatat
     7321 taagcttttc gaaatggacg gggtgacttt gagctttgac gatgatgtcc ttgaatacat
     7381 tgtagacaag gctattgaat ttaagcttgg agcaagaggt ttgcgttcca tcgccgaaac
     7441 catcatgatg gatatgatgt acgaaactcc gtccgggcgc agaaaaaata tccgtataga
     7501 tttaaaatac gctcgatcca aaattgaggc agctaaccag tccaggctca gggaacaata
     7561 gcatgtttca gaaaaataat tctagttttt ccctttattt gttggtattc tgaaagattt
     7621 gtttttaaat ttgtttgtca gcaaaaaaac gtagttatgg caaaatccaa tcctttggca
     7681 gaaagtctta agttgcattt tggattcgac actttcaaag gaaatcaaga agctatcatc
     7741 cggaacataa tggaaggcaa ggacactttc gtgctcatgc ctaccggggg tggaaaatct
     7801 ttgtgttatc aattgccttc tctgcttttg aatggcacag caatagttat ttcccctctt
     7861 attgcactga tgaagaatca ggtggatgcc atgcgtaatt tcagcgacga agacggagtg
     7921 gctcatttta ttaattcttc cttgaacaaa gccgccatcg atcaggtacg caacgatatt
     7981 acgtcgggca aaaccaaatt gctctacgtg gctcccgaat ctctgaccaa agaagaaaac
     8041 atcgattttc taaaacagat tcccatttcg ctctttgcag tagacgaagc acactgtatc
     8101 tcggagtggg gtcatgactt caggccggaa taccgacgta tccgtccgct tattgatgaa
     8161 atcggcagag cacctatcgt agcgcttaca gccaccgcca cgcctaaggt gcaacacgat
     8221 attcaaaaaa acctgggaat gatggatgcc actgttttta aatcatcctt caaccgcccc
     8281 aatctttttt acgaagtcag agccaaaacc tccagcatcg ataaagaaat cattaaatac
     8341 atcaaggcca atcccggcaa atcgggtatt atttattgcc tgagccgcaa aaaggtggag
     8401 aaactggccg aaaccctgca agtaaacaat atcaaagccc tcccttatca cgccggtatg
     8461 gattcacaaa cccgaacagc caaccaagat gcgtttctga tggaaagggt agatgttatc
     8521 gtagcgacaa ttgcctttgg gatgggtatc gacaaatcgg acgtacgctt tgtcattcat
     8581 tacgatattc ctaaaagtct cgaaggttat tatcaggaaa cagggcgagc cggacgcgac
     8641 gacggcgaag ggtattgttt ggctttttat tccaacaagg atctgcaaaa actcgataaa
     8701 tttatgcagg gcaaacctgt ggccgaacag gaaataggta aggagctttt actggaaact
     8761 gcggcttatg ccgagtcatc ggtgtgcaga aggaaatctt tactccatta tttcggagaa
     8821 acttacgata aagaaaactg cggtaattgc gacaattgca taaatccaaa aaaacaagtg
     8881 gaagctcagg aattactttc tatggttttg gaaaccgtcg ttgctgtcaa agaaaaattc
     8941 aaaacagatc acattattga tgttttatcg ggcaacgaaa cctcggacgt tgtttcctat
     9001 aaacacaacg aactggaatt gttcggcatg ggagccgaag aggatgaaaa aatctggaat
     9061 gccgttattc gtcaagccct tatcgatggc tatctggata aggaaatcga aaattacggc
     9121 ttactcaaga ttaccgactc gggcagaaat tttctgaaaa aaccgcgcag cttcaaggta
     9181 accgagcaat tggattacga ggaaatggaa gaagatatgc aaatgcacgg cagtgcaacc
     9241 agcgccgtag accccatcat gttttccatg ctcaaaaatt tgcgcaaaaa aatatccaag
     9301 caactcaatc ttccacctta cgtcattttt catgatcctt ccctggaagc catggccacc
     9361 acctacccca tcacaatgga agagttgcaa aatattcccg gagtaggggc cggcaaggcc
     9421 aaacgttacg gacaggaatt tctttcgctc atcaaaattc acgtggaaga aaacgaaatc
     9481 gaacgtcccg aagatttgcg cgtgcgcacc gtagccaata aatcaaaact gaaagtatcc
     9541 attattcagg ctatcgaccg caaggtggcg ctggacgacc tggccaatac caaaggcctg
     9601 ggcttttcgg aattactcga cgaaatcgaa gcaatcgtat actcaggcac cagaataaac
     9661 atctcttact tcttgatgga gataatggac gaagaacaca tcgaagagat tttcgattac
     9721 ttcaaagatt catccagcga cagcatccag gaagccatgg atgtgctggg tggcgattat
     9781 accgaagaag aaattcgcct ggtccgcatt aaatttattt ctgaatacgg aaactaaaca
     9841 acgtatttac gcgtttgaat gataatgctc acattatact gattgtctta tgagaaaaat
     9901 tttgttagcc tgtctgttat ctgttatttt ttcaaatgtc cgggctcagg ccgatgaccc
     9961 ggtagtaatg aacatcaacg gggaagatat actcagatcg gaatttgaat acaactggaa
    10021 aaaaaattac aacatcagtt ctacggacaa aaaaaatctg gacgaatacc tggagctctt
    10081 cgtaaacttt aagcttaaag tggccgaagc caaagctcag ggaatagaca ccactcaagc
    10141 ctttataacc gaactcaggg gctaccgcag tcaattgacg gctccctatc ttaccgataa
    10201 acagaccgaa gaaaaattaa tagacgaagc ttatgccttt acgagtcaat acgttgattt
    10261 gagtcatatc ctgattccgc tggacagaaa tgcagctccg caagataccc tgacagtgta
    10321 caaccagctc atggagttaa gggaacgcat tttaaaggga gaagattttg gccgcctggc
    10381 tgcagagtat tctcaagata attcgagccg cgacggaggc cgcatcggaa ctttcctgtg
    10441 ttccaggcta atctatccca tcgctaaaac cgccttctac agccaaccgg gagaaatagg
    10501 cctgcccgtc aggaccgcct tcggctatca tctgataaaa ctgaatgcca aacaccaggc
    10561 tcccggacaa tacatctcgg ggcatattct tcaatccgct cccagcggtg ctcccaagga
    10621 ggtggtcgat tcggcaaaac aacatattac cgaaatctgg ggcttgctcg aaagcggaca
    10681 agattttgcc cagttggccg acactcgcaa cgacgaccgc tatgtggtgg gtaaaggagg
    10741 aaaataccag gaactaaggc cgggatcgct gccctatgaa tacgaacaac aggtgtttga
    10801 tttaaaagac ggcgagtatt cccaaccttt taaaaccgaa tacggctggc atattgtaaa
    10861 acgcttcgaa atcactcccc tgcccgaaaa aggagaaatc cttgaagaac tcaaaagcac
    10921 tttgcagcgc gatgaaagag ctcaggcagg caggaaggcc ctggtaaacc aattgaaaaa
    10981 agactataat tatcaaccgg ataccaaggc cctggctcaa tttgaacaaa ccctggccgg
    11041 actcgaaaat ccggactcta cgttgcgggc agaagcggcc cgactgcccg tgctggcaag
    11101 tttcgaacaa aactccataa cgcccacgga ttttgctgaa tactatttgc aaagaaaatc
    11161 cctgatcacc tccgttccta cggcttttga agactacgtc gaacagagga tactggccta
    11221 cgaagacagc cggcttgaaa agaaataccc ggattttgga aatctgatgc gtgaatacca
    11281 cgatggcatt ctgctttttg aaatcagcaa tcaaaaggtt tgggaaaaag cagtagaaga
    11341 tcaggagggt ctggcggctt acttcaaagc caacaaagcc gactatcaat gggacaaacc
    11401 caggttcaaa ggagccctta tccattgtgc cgacgagacc accgccaaaa aagcaaaaaa
    11461 aatggccggg aaattgaagg acgattcact ggcttttgaa ttaaaacgta gcttcaataa
    11521 cgacagtacc acactgatca aggtggaaca agggatttac gtgatgggag aatatgcccc
    11581 ggtagataaa ttggttttca aaagcggcga ttggaatccg gacaaatcgt tcccgatcgt
    11641 atttaccaag ggtaaagtcc tgaaaaaagg acccgaagaa tattccgatg tacgcggagc
    11701 agtcatcggt gattaccaaa actacctgga aaaggaatgg attaaagaac tcaaacaaaa
    11761 atatcccgtc agtatcttcg aagaacacct ccagatccta aaagcagaat aaacaaatac
    11821 tgagggtttt ttagtatctt tgtactcttt ttatgttaga ctatgaaaca tctactctgg
    11881 ctgttgttgc ttatgcctgc ctggctgcag acatgcctgg gcaaagaaac cgaatcgccc
    11941 agagttgccg ttgtccgggt ctcggacgct accctttacc ttgacgaaat tcaaaacgct
    12001 ctgccaacag gcctgaattc agtcgacagc atacgttttg tcaattccta caccgaaaac
    12061 tgggtaaaag aaaccctttt ataccaggct gccagtaaaa atctgggcca aagtcctgaa
    12121 ataaacagaa tggtagaaaa ctaccgccgc tcgctcatta tatatcaata tcagcaacag
    12181 gcgctgaacg aaaaaatgga atcggaggtg accgaagccg atatggcaga gtattacgaa
    12241 aaaaacgcca atcgttttat ttccaacaaa aatctgatta aaggattgtt tatcaaagtt
    12301 cccctgtcgg ctccgggact ggacaaattg aaaatctggt acaaaaaaaa cgataccgaa
    12361 tcgctggaca atattgagaa atattcgtat caaaacgcaa gtatttatga atatttccac
    12421 gagacctgga ctagtctgga cgatgtaatg gatcagattc cggccaatat agccgaccag
    12481 gaaaaatttc tccgcaacaa taagtttctc gaagtcgaag acggaaatta ctgttacctt
    12541 ttgtatataa gcgattacat tacaaccggc agccctcagc ccttggacta cgtgagcgcc
    12601 caaataagaa acattttagt caacagcagg aaaacagaat tcattcatca atttgagaac
    12661 gatatgcttg aacaagccaa acgcaagggg gcaataaaat acttttccat tcctgttgtc
    12721 gacaccgcct ccgctcaaga tttaaccatc gatccacaat gaaacagttt atatacctaa
    12781 tagcccttat actggccttc gcattacaaa gcttgcaagc ccaaaacaac atagttgatg
    12841 aaattgtctg gatagtgggc gatgaagcta ttctgaaatc cgaagtggaa gcctatcgtc
    12901 tgcaggcttt gcaggaagga caaaagttca gcggcgatcc ctattgtgtc atacccgagc
    12961 aactcgctgt tcaaaaatta tttcttcatc aggcggaact ggacagtatt atagtgaccg
    13021 acaacgatgt aatcaccaat gtggaccgca acatcaaccg tatgattacg gtactgggtt
    13081 ccaaagaaaa aatggaagaa tattttggta aacctatttc caaaatccgc gaggagcaaa
    13141 gggaatacgc cagaaatgaa caaaccatac atctgatgaa gcaatccctg ctcaaaggtc
    13201 tgagcgttac tcctgccgaa gtgcgtgctt attataaatc gctgccctcc gacagtattg
    13261 ccatcatccc ggccgaaatc gaagtggaga ttatcacagt ggaacccaga attccggagg
    13321 ctgaaattga atccatcaaa gaacgcctgc gtgaatttac cgaaagagtc aacaacggcg
    13381 aaagattttc taccctggct gttttgtact ctcagcacga agccacggct aaattcggag
    13441 gagaaaccgg tttttacggt aagggagaac tgatgcctga atttgccgcc gtcgccttta
    13501 gtctgacaga ccccaccaaa gtgtcgcgca ttgtggaaac cgacgacggt tttcatatca
    13561 ttcaattgat cgagaaaaga ggagatcgtg tgaattgccg acatattttg cttaaacccg
    13621 aggtttcagc taccgaaaga aacgaagcaa tggtacgtct ggatagtatt gccgatttta
    13681 ttcgcaaggg taaattaagt ttcgcggatg cggccatgtt ttattcgcaa gacaaaaaca
    13741 cccggaaaaa cggaggattg atgttcaatc ccaatacggg gaccgctcat tttcaactcg
    13801 aagaattgcc tcaagaagtc agcaaggtgg tttactcgat gaatataggc gaaatttctg
    13861 ctccctttag tatgcgtaat cccgacaacg acagggaaat atgcgccata gtgcgggtca
    13921 aaaacagaac caaggcccat aaagcaacgg tagaagaaga tttcgtgaaa ttaaaagata
    13981 ttgtcttaca aaataaacaa gaaaagctca tccgggaatg gatcgtaaac aaacaaaagg
    14041 ggatttatgt tcgtatcgat gaaagatggc ggaactgcga tttccaatat ccgggatgga
    14101 ttaagtaata tgttcaatgt tgccgacata aagcctgcat tgcggcatat gagccttggt
    14161 ttattgtgcc tctgttctct tgttttgcag gctcagggga acagaatatt ccttgaacac
    14221 gctaacagca ccaattttga caagaacatc agtcccgact atcatttttt gataggcgat
    14281 gtacaattca ggcacgacag tgcccgtctg tattgcgata cggctcatta ttacagagcc
    14341 gataattctc tccgggcttt tggaaatgtc agaattgaac agggcgatac cctgtttatt
    14401 ttcggtaaaa ccctttttta cgacgggaat aaaaaactgg cccaactccg gaataatgtt
    14461 cgcatggtca acaaagaggt gaccttattt accgaccacc tggattacga cagagtggcc
    14521 aacattggat attactacag aggcggaaaa atcgttgatg cttccaatac ccttagctca
    14581 gattacggac gctacaaccc cgacaccaaa atggcttttt tcaaaaagga tgttgttctg
    14641 actcaccctc agtttctgtt gaaaaccgac actttaaatt ataacaccga aacaggaacg
    14701 gcctccatta tttccagaac cgaaatcgaa gccgaaaatg cagatataat agcctaccgg
    14761 ggctggtaca ataccaaaac cgataaaagc ctcttgttgg atcgttccta tattatcagt
    14821 gaaaaacgca gcctaactgc agattccatt ctttacgaac agtcgctcga catcggtgaa
    14881 gcctttgtga acattgaatt gatagattct tcgagaaata ctacgctaac ttccgattac
    14941 gccttttacg atcaaagcaa caattacgtc ctcttaacca aaaaggccct gctcagagaa
    15001 tactccggcc aggacacctt ttatttgcat gccgacaccc tgctgggaaa acaagactct
    15061 atttacgaca ccttcagagc ttattacaat gtcaggagct atcggagaga tttacaggga
    15121 ctctgcgatt ctgcctttta ttcaacgcgc gactccgtac tccagctttt cggcaatccc
    15181 atcctttggg cggacaatca gcaaatcagt ggacaacaga tccttctgta caccaaaaat
    15241 caacaacccg actatatgga ggtggagggc ttggctttgc tggcatccaa agaggacagc
    15301 ggcctcttta atcaatcatc gggcagaaag cttaccgcct attttctgga caacaaggtg
    15361 cgacgggtcg ttatagaagg taatccggaa tccatctacc ttccccgcga cgaaaaacaa
    15421 aagatattag gtatgaaccg cctggaaaac ggttctttgt acatattcat ggacgaagag
    15481 ggcaaagtgg aaagacttaa agtgagccct caacccaaag gaaagtttta tcctttgcaa
    15541 ctgctcaccg aggaagtgaa gtatcttgag cacttcagct ggcctataca cctgcgcccg
    15601 acagatcctg cggatgtttt ccgtctgacc gaccggccaa gagccgaaaa catgagcacc
    15661 aacattggag gtgctgccgg cgagggtgca aacgctcccg gcagggcagc actcagaaca
    15721 tttaaaagta aaaacagtaa gtaatatgtc gttttttaag caaagaaagc ccagaaaatt
    15781 tgatcatcaa cccatttatt acgatgctca aaaagaagct cgtcaggagc gcttgcaaaa
    15841 agctaaagta gaattggggc tgatggatga aaaagagcta aaaaaagatt atgaaaaagt
    15901 cattcgaggc agtttcagag aagctgtccc gggtggaagt cgcaccaaac gaaaacaaga
    15961 cagcaacaaa acgatgagaa ttcttgtcgt actgttcata atcatcctcg tggtatattt
    16021 tttagcttct cgttatgctg gataaaaacc tggccggcaa cactatacgg gttttgcccg
    16081 agtcggtggc caaccaaatt gccgccggag aggtgattca gcgtcctgcc tccgctgtaa
    16141 aagagctgat ggaaaatgcc atcgacgcag gagccagcca gatcgacgtg ctggtaaaag
    16201 atgccggaaa gagcctgatc caggtaacag acaacggtaa gggcatgtcg ccccaggatg
    16261 caatactggc tttcgaaagg catgctacct ccaaattgag tgtggcaact gacttactaa
    16321 aaattcgcag catgggcttt cggggcgaag ctctggcttc tatagcctcg gtagcccagg
    16381 tggaattgca aagcaaaaga cctgaagacg aactgggaac caaagtatcg atagccggct
    16441 ccaaaatcga aaatcaagaa acagtgcttt gtgcttcagg ctcacgcttt tcggtacgta
    16501 atctatttta caatttaccg gctcgtcgaa aatttctggg tagtaacgcc aaagaatggc
    16561 tgcacgtgcg tagtgaattc attcaaattg ccctggccta ccccgaatta caactcagcc
    16621 ttgtccacaa tggcgaaagc attttcaggc tatctccttc gggattgaga caacgcattg
    16681 ttgctattta tggtgaacag ctcaacaaaa aattattccc catcgaagtg gaaacggaaa
    16741 tcgttcgaat cagcggtttt gtaggatcac ccgatgcagc acgcaaaaga gcctccgagc
    16801 agtttttctt tgtaaaccgg
//
LOCUS       DAOSGL010000010        16669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_2818_length_16669_cov_3.990430, whole genome shotgun sequence.
ACCESSION   DAOSGL010000010 DAOSGL010000000
VERSION     DAOSGL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16669
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_16669_cov_3.990430"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKY68_00740"
     CDS             complement(<1..917)
                     /locus_tag="PKY68_00740"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOH23600.1"
                     /translation="MKRTAIVLLSFLLLGTFSCRRAASLDEVFLNPPDTAKPWIFWYW
                     INGAVTKEGITADLEAMKEIGLEGAYLMPIRDSSRVQYMSNTVLQLSDEWWEMVRFSM
                     KEAGRLGLKMGMHICDGFALAGGPWITPEMSMQKVVYSETLVKGGRIEQLKLDKPETD
                     IDYYRDIAVFALPAPALESSENLRPKVSSSDRQELSFLLDGSGRFKAKDDCWIQYTFD
                     QAFTARSLTIVTGGNNFQAHRLRVAVSNDGKNFCDVKQLVPARRGWQDTDADATYTLP
                     ETTARYYRFYWSPEGTEPAAEDIDPAKWKP"
     gene            complement(1021..4251)
                     /locus_tag="PKY68_00745"
     CDS             complement(1021..4251)
                     /locus_tag="PKY68_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH23601.1"
                     /translation="MKKNIGLLIAGLIIGSCLCSAQAFRPLWENHRVLEINRLPARAS
                     YVPYLEEKQDRTMSLNGNWKFQYVPKVEEAPTSFYRNDYDDAGWAEIPVPSNWELHGY
                     GTPIYISAGYSFKIEPPLVSSEPDPRYTSFSERTPTGSYRRTFELPAAWSGKKIFLRF
                     DGVMSAFNVWVNEQYVGYSQGSMEMSEFDVTGCVKPGKNLIAVQVFRYSDGSYLEDQD
                     MWRLSGIYRDVSLYATEKIRIYDFGVRTELDDTYTNARLLIEPKLSVYDGQTADGYVL
                     RADLYDAEGKAVFEQTLEQDAVPVLNREIRSNILNARTPQRGARSFGWFDVEVASPHL
                     WTAETPYLYTLELGLYNEGGEAVEKIQTKVGFRSVEIKQGQVLINGKPIRFRGVNRHE
                     HDPFTGKVVSEERMLQDILLMKQANVNAVRTAHYPNVNRWYELCDQYGLYVMDEANIE
                     THGLRGFLASEPDWHAAFMDRAVRMVMRDRNHPSIVFWSMGNESGYGPNFAAISAWIR
                     DFDPSRKIHYEGAQGNIKDPETVDVIARFYPRVQDEYLNPNIPEGSDAERPENARWER
                     LLSIARNTADDRPVMTSEYVHCMGNALGNFKEYWDEIYSHPRMLGGFIWDWVDQALAK
                     PLEDGRVQMSYGGDFGDYPNSKAFCMNGVILAERQTTPKYWEVKKVYQPALFEQGKDE
                     YHYRITNRNHHTNLDQYNLSWQVISDGKTIETGNMDLPAVAPGDSHELKLPLTFGKKY
                     RGDVRLFLSLQLKEDALWAKKGHELAWEEFNLKKAALTAPELKKGKIGIDDKEQILIV
                     EGKDFSQQWDKQTGALVSLVFKRQEMLSREEALFPQPNFQAYRAPTDNDAGFGNWLAK
                     DWSTHLMATPQRKLLNFSCNPEPDGPVEIKTESLYTYLKGTIRCTSVFRIGADGSMDV
                     EHQFHPEGELPVLPRLGVAWAFAEDLQQLQWYGPGPLETYPDRKTGMRTGLWSSTVAD
                     QLFPYPRPQESGNHEETQYLILKNSRNKGLKITAVEDVFSFSALPYLATDLSNCRHYT
                     ALQARKAVICSIDAQQLGLGNSSCGPGVLKKYTVKKTQVRYRIEGL"
     gene            complement(4262..6328)
                     /locus_tag="PKY68_00750"
     CDS             complement(4262..6328)
                     /locus_tag="PKY68_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOH23602.1"
                     /translation="MKKTIMLCALLACTGLYAQDKGLVNTTKSPYARIWNTDVSAVSW
                     TDGFWGERFEVCRDTMVINMWHVMSDPEVSHAFRNLEIAAGEAEGRHSGPPFNDGDFF
                     KWFESAAAVYAVTKDPKLDALMDRIITTIAKAQREDGYMHTQVIIPERKRQKEQASQQ
                     SGPVGTAVGTAQGGAFSDRLNFETYNHGHLMTAACVHYRATGKRNMLELAIKAADFLC
                     RFYETATAELARNAICPSHYMGVVEMYRTTGDPKYLDLSKNLINIRGMVENGTDDNQD
                     RVPFRQQKLAMGHAVRANYLYAGVADVYAETGEALLLENLESIWSDIVYRKMYITGGC
                     GALYDGTSPDGTNYTPDSIQKVHQSYGRAYQLPNSTAHNETCANIGNMFFNWRMFLLG
                     GDAKYVDVLERTLYNSVLSGISLGGTKYFYTNPLRLSKDFPYVLRWPKERTEYISCFC
                     CPPNTVRTICEAQNYAYSLSKEGVWLNLYGSSNLATKLADGSDIEIVQRSDYPWDGKV
                     ELTFAKLPRKADFSLFFRIPGWCDHYELSINGNPVELQVEKGYARVQAKWKKTDNITF
                     NMRIQPRLIESNPLLEETRNQTAVMWGPLVYCLESADLGNYKIDNISIPSDISFQPEM
                     MEIDGSRLMSLKGEAWYVDQQNWDNVLYREVSSAKKKVPVRLIPYYAFGNRGFEEMTV
                     WMPLDR"
     gene            complement(6341..7876)
                     /locus_tag="PKY68_00755"
     CDS             complement(6341..7876)
                     /locus_tag="PKY68_00755"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOH23603.1"
                     /translation="MTDKYILKGDPQGEEWAPIFTTHGFRYVELSNYPGEPKPENFIG
                     EFVSDEMEDLGYFESSSEVLNGIVRNAYWGIKGNYKGMPIDCPQRNERMPWLGDRTIG
                     AYGESFLFENDKLYAKWMDDIREAQRWDGSIPDVAPAYWNYYSDDVTWPAAFFFISEM
                     LYRQFGDLRPIEKNYESMKLFMEHMRRHYLKDGLLVADKYGDWCMPPEAPEIIHSKDP
                     SRITEGALISTAYFYKLYHIMSGFANLLNKPEDSMVYLIMAQDMKTAFNRHFYNSEKQ
                     YYGNNTVTANLLPLAFDLVPAEARKGVEENVQKTLLETYGGTMATGVIGGSWLMRELT
                     AMGRGDIAFLLASTTKQPSWGYMLTKGATTIWELWNGDTAAPSMNSGNHVMLLGDLLI
                     WCFENLAGISSDTEQVAYKRIVMKPDFGIADLNFINASYKTPYGLVVSNWKKSEGSLD
                     WSIKVPANSSALVYLPVVRKKNIKESDRPIGSSRTIRYLGTENNGTLIELGSGEYHFN
                     VQL"
     gene            complement(7866..9119)
                     /locus_tag="PKY68_00760"
     CDS             complement(7866..9119)
                     /locus_tag="PKY68_00760"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOH23604.1"
                     /translation="MKRLILISALVSLVFISCKQDQSSAPDLKAVELSCEMLKDPVGI
                     DAPNPRLSWKLQTEARGVRQTAYRLMVASSEEKLAVGQADVWDSGKQNSDASILIPYA
                     GPELRSSRYYYWKVQIWTNANKEAVESETARWATGLFTENDWKNSRWIGLDGPMPWDV
                     EKAHARLSARYYRNEFNCAKPVRQALVHISGLGLYELWINGGRIGNHVMTPAPTDYRR
                     SILYNSYDVTSSLQQGQNALGVIVGNGRYYAMRQNYKPYKWANFGYPKLRMILNIEYE
                     DGTRQTIGSNERWMMTADGPIISNNEFDGEEYDARKEFPGWTEVGFKTGRNWFPAQRV
                     TIPTAVIRAQPNPEMKVSDVFHPQSITKINKNTYILDMGQNMTGWIRMKVKGNAGQEV
                     KLRFAEILQEDGSLSMANLRDAGDG"
     gene            complement(9125..10567)
                     /locus_tag="PKY68_00765"
     CDS             complement(9125..10567)
                     /locus_tag="PKY68_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HOH23605.1"
                     /translation="MKLKNLILKNSLLVLTLVLLACGQKEELPWDNGKLQVDATQCYL
                     QHENGKPFFWLGETGWLLPERLNRLEAEFYLENCRQAGYNVVQVQTINGVPAVNTYGQ
                     LSMIDGFDFSKINQEGVYGYWDHMDYIVETAESKGIYIGMVCIWGGLVKRGLMDEEQA
                     RAYGTFLAERYKDRPNIIWFIGGDIRGDVKPEVWHALAESIKAIDKNHLMTFHPFGRT
                     LSAIWMHNAEWLDFNMFQSGHRRYEQSEPGKEFLPDSTEEDNWRYVEISKTYEPLKPV
                     IDGEPSYEGIPQGLHDPKEPLWQAKDVRRYAYWSVFAGSFGHTYGNSSIMQMYKGGPR
                     PGAYGATVHWVEALKDEGFNQMKYLKNLILSFPFFERIPDQSVIAGENGVRYERLSAT
                     RGNDYLLVYNYTSRPMEIDLTKISGQTKSVWYMDPTSGALSYLGEFENGVQLFQSPKP
                     QAEGNDIVLIAVDAAKKYVDKSWTVLPDLK"
     gene            complement(10580..13411)
                     /locus_tag="PKY68_00770"
     CDS             complement(10580..13411)
                     /locus_tag="PKY68_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOH23606.1"
                     /translation="MKNRHSKSIRFVKTRSYISRFQLLLGLLLLAGSLSSCTPEIRIG
                     KMSLDYQEGPLALQNKDFRLGWQILASGNSVVQEAYELEFYRWEASQKSLLWSSGKVT
                     ENNSQQVLYEGPELQARQKYAWRVRIWDQQGRTTAWSKQQVFWIKPEEAWLDAQWIGA
                     ISRADSKLPEGRQYEGNLVNRDPESKALWAATDPLSKQSLYLRRALNLGKPIEEALVY
                     ISGLGHYELSLNGQKVGDSEFAPLWTDYDKTVYYNVYDVKDLLKKGENVAGVLLGNGF
                     FNVQGGRYRKLLVSFGPPTLFFKLFVRYTDGSTGEWISDQNWKYDFSPITFNCIYGGE
                     DYDANLEQTGWDKPGFDDSNWRSVVVQEAPKGVLLAQTANPVKIMHRYPVKSMQRLAM
                     PPARNRNSGEAQAEKGDTVWVYVLDMAQNLSGYPEIKVKGRKGDKVKLTLGESLNDRG
                     LVSQSQSGGPHYYEYTLKGAGEEYWRPRFSYYGFRYIQVEGAVMRGGENPDNLPVINE
                     INSCFVHNSAENIGHFESSNTIFNNAHKLVNMAVRSNMQSVFTDCPHREKLGWLEQVQ
                     LCGQGLMYNYDLSRLFPKVMRDMADEQYPNGLVPTTSPMYVEFSELWNDSPEWGSTSV
                     ILPFLYYWRYGDDRLIREYYPVMKKYVDYLESRSDNHIVMHGLGDWYDYGDFKSGFSR
                     NTPVPLVGTAHLYLDIQLLIRAATMLGIRGDIDYYTQLGNEVNKAFHERFFRADSCIY
                     GTGSQTSYALPLFLDMVEPQYRQRVLDRLVADIEAHGNRLTTGEVGNRYMFQVLARNG
                     LNELMYKMHNHEETPGYGFQVKFGATTLTEQWDPRQGASWNHFMLGSIDEWFFRSLGG
                     IQFDHEQAPGAQHLIIKPEVVGDLTRVKCRTQNLYGTIGVEWELDEDKQFAMKVEIPT
                     NCDAKVFLPGEDMPELIQSGKHQFIKQLP"
     gene            13686..15056
                     /locus_tag="PKY68_00775"
     CDS             13686..15056
                     /locus_tag="PKY68_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOH23607.1"
                     /translation="MIDSKIEERLQEMRAFYRTNTTKDYRYRIHSLKTLSSSIAKHEN
                     ELMEALAADLHKSHFESFATEISIVQQEIRFQIRHLRKWSRTRRLATPMVFFPSRSYL
                     YKEPYGVVLIIASWNYPFHLLFNLLVGALAAGNCAVLKTSPLAPATAGVMEKIVSCFP
                     VDYINIWHGHREVNQALLKARFDYIFYTGSPAVGKVVMEAASKHLTPVTLELGGKSPC
                     IVDKDANIRFAARRIAWGKTINAGLTCIAPDYLLVHHSVKEEFLLEYKQAIFSMFGSD
                     PQASPDYPRIVSSSALARLSGYLKEGKIVLGGAIDPEDLYLEPTVLEQAKPEAGVWQE
                     EIFGPVLPLKEFDQIEEVLQFIYKREKPLALYYFSRNKENIRLIVKSTSSGGVCINDT
                     LLHVANHHLPFGGVGHSGMGQYHGKYSFETFSRNKAIVCSSSNIDIPLKYPPYRAKLK
                     LLRKLF"
     gene            complement(15107..>16669)
                     /locus_tag="PKY68_00780"
     CDS             complement(15107..>16669)
                     /locus_tag="PKY68_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH23608.1"
                     /translation="TCFIPTNDAMRAYYADSSKSGFEEFSVDVLREMVYYQIIDTKKY
                     LSTDFPQGRLSNKNMMSRFIDISFEKYAEEGGILVNSSSTIKLKDQEVHNGVIHVIDK
                     VLVPSTYLLPESIRSDSAFSIFAEALYLTCMSDSLLGIRDDSYVKPISFLNAAGATVA
                     TPPDRLYGYTALVEPDSIYAEYGIYNLDDLIAFAASVYDEVFPEDAVYAGDFTHRKNS
                     LNRFVSYHLFDYMCYYNEFVDYYYNHGGMMEGKEGVEYIQTMCPNTLLEVRAGNRFNV
                     QSNGEHIRILGKGMKHRACVNGIFHPIDKILLYDGTVINDVLNKRLRMDVYSTLPEMM
                     NIRARGVSAEREYYYPSGFFKNLIYKDDETKVNGKRPTGGAVSLQGDRFHIYGWYDFT
                     HILPPVPEGSWEIRIGIKTREKNIVQIYVDGIPNGIPLDMGKNAHHPDVGWVADIDTE
                     DNGAINDKDLRNRGWMKAPEYFCMYPSGRSGRDDWNSLRRILGIYTLGDGKTHTFRMK
                     SVLSSNTSDYFG"
BASE COUNT         4243 a         4205 c         3737 g         4484 t
ORIGIN      
        1 ggtttccatt tggccggatc tatgtcttcg gctgccggtt cggtaccttc gggtgaccaa
       61 taaaaacgat aatacctggc ggtggtttcg ggcagtgtat aagtagcgtc cgcatcggta
      121 tcctgccagc ctctgcgtgc gggaacgagc tgtttgacgt cgcagaagtt ttttccgtcg
      181 ttgctgacgg ccacacgcaa acgatgcgcc tgaaagttat tgcctcccgt cacaatagta
      241 agcgaacggg cagtaaaagc ttgatcgaaa gtatattgaa tccagcaatc gtctttagcc
      301 ttgaagcggc cggaaccgtc caacaaaaag ctcaactctt gtctatcact ggaactgact
      361 ttgggccgga ggttttcgct gctctccaat gcaggagcag gcaaagcaaa aacggcaata
      421 tcgcggtaat aatcgatgtc ggtttcaggc ttgtccaatt tgagttgttc aatccggcct
      481 cctttgacca gcgtttcgga atacaccacc ttttgcatgg acatctccgg agtaatccag
      541 ggaccgccgg caagggcaaa accatcgcag atatgcatac ccattttcag acccaaacgg
      601 ccggcttctt tcatggaaaa acgcaccatc tcccaccatt cgtccgagag ttgcaatacg
      661 gtgttggaca tatactgcac tctggaagaa tcacgaatgg gcattaaata ggcaccttcc
      721 agcccgattt ctttcatggc ttccaagtcg gcagtgatcc cctctttggt caccgcgcca
      781 ttgatccaat accaaaaaat ccagggtttg gcagtgtcgg gaggatttaa aaaaacctca
      841 tccaacgaag ctgcccgccg gcaggaaaaa gtacctaaaa gcaaaaagct taataggact
      901 atagcagttc tcttcatata acttattctt tttgtttcat tgttgcctgc cccgatgctc
      961 tcaagggccg tacaacaaat aatttgatta ttgtatcact tacttgttat tgttcctgtg
     1021 ttacaagcct tcgatcctgt aacgaacctg agttttcttt accgtatatt tttttaaaac
     1081 gcccggtccg caactactgt tacccaagcc taactgctgg gcatcgatgg aacatatcac
     1141 cgcctttcta gcttgcaaag cggtataatg acgacaattg ctcaggtcgg tagccagata
     1201 cggcaaagca gagaaagaaa aaacatcttc tactgccgta atttttaaac ctttattcct
     1261 gctattctta agaatcaaat actgcgtttc ttcgtgattt ccactttcct gaggtctcgg
     1321 atatggaaat aactgatcgg ccactgtcga cgaccacaac ccggtgcgca tgccggtttt
     1381 acggtcggga taagtttcaa ggggccccgg gccgtaccat tgcagttgct gcaggtcttc
     1441 ggcaaaagcc caggcgactc ccagacgcgg cagaaccggt aattcccctt cgggatggaa
     1501 ttgatgttcg acatccatac ttccatcggc tcctattctg aagacggaag tacagcgtat
     1561 ggtacctttg aggtaggtat aaaggctctc ggttttgatt tcgaccgggc cgtccggttc
     1621 aggattgcag ctaaagttaa gcagcttcct ttggggcgta gccatcaagt gagtactcca
     1681 gtctttggcc aaccagttac cgaagccggc atcgttgtct gtcggggctc gatatgcctg
     1741 aaaattcggc tgaggaaaca atgcttcttc gcgggagagc atttcctgcc gtttaaatac
     1801 cagagaaacc agggctcccg tttgtttatc ccattgctgg gaaaaatcct taccttcaac
     1861 gatcaaaatc tgttccttgt cgtcaattcc gatttttcct tttttgagtt ccggagccgt
     1921 caaagcggcc tttttcagat taaattcttc ccaggcaagt tcgtggcctt ttttggccca
     1981 cagggcatct tctttgagtt gcagactcaa aaaaagacgt acatcgcccc ggtatttttt
     2041 tccaaaagtc aaaggaagct tcagttcatg gctgtctccc ggcgcaacgg cgggcaagtc
     2101 catattgcct gtttctatgg ttttaccgtc ggaaatcact tgccagctta agttatactg
     2161 atcaaggtta gtgtggtgat ttcgattggt tatgcgataa tgatactcgt ctttgccttg
     2221 ttcgaacaaa gccggctgat agaccttttt cacttcccag tatttaggcg tggtttgccg
     2281 ctcggccagt ataacaccgt tcatgcaaaa agccttggag ttaggataat ctccaaaatc
     2341 gcccccgtag gacatttgaa cccgtccgtc ttcgagaggt ttggccaaag cctgatcgac
     2401 ccagtcccaa ataaaaccgc ccagcatacg ggggtgactg tagatttcgt cccaatattc
     2461 cttgaaattc cccaaggcgt tgcccatgca atgcacgtac tcgctggtca taaccggacg
     2521 atcgtcggcg gtgttgcgtg ctatactcaa aagcctttcc cagcgggcat tctcgggacg
     2581 ttccgcatcg gaaccttcgg gaatattcgg attcaggtat tcgtcctgaa cccggggata
     2641 aaaacgggca ataacgtcta ctgtttcagg atcctttatg ttgccctgag ccccttcgta
     2701 atgaattttc cggctgggat caaaatcccg tatccaggcg gatatggccg caaaattcgg
     2761 tccgtagccc gattcgttac ccatagacca gaaaacaatg ctggggtgat tgcggtcgcg
     2821 cataaccata cgcacagccc tgtccataaa agcggcatgc caatcgggct cgctggccag
     2881 gaagcccctt agcccatggg tttcaatatt ggcctcatcc attacgtaga ggccgtattg
     2941 atcgcaaagc tcgtaccagc ggttaacgtt gggataatga gcagtacgaa cagcattcac
     3001 gttggcctgc ttcataagca ggatgtcctg caacatacgt tcttcgctca ccaccttgcc
     3061 ggtaaacgga tcgtgttcgt gacggtttac tccgcgaaaa cgtatgggtt tcccgttaat
     3121 aagaacttgt ccctgcttaa tttccacact acggaatccg actttcgtct gtatcttttc
     3181 taccgcctcc cctccttcat tatataaacc caattcgagg gtatagagat aaggggtttc
     3241 ggcagtccac aagtgagggg aagctacttc cacatcgaac cagccaaaac tgcgggcacc
     3301 gcgttggggc gttctggcat tgagaatatt ggaacgaatt tcacggttga gcaccggcac
     3361 agcgtcttgt tccagggttt gttcaaaaac ggctttccct tcggcatcgt acaaatcggc
     3421 cctcagcacg tatccgtctg ccgtttgtcc gtcgtatacc gatagtttcg gttcaatcaa
     3481 cagacgggca tttgtgtaag tatcatccag ttcggtacgt acaccaaagt cgtaaatgcg
     3541 gatcttttcg gtagcgtaca acgacacatc ccggtaaata cccgacaaac gccacatatc
     3601 ctggtcttcg aggtaactgc cgtcgctgta gcgaaatacc tgcacggcta tcaggttttt
     3661 cccgggcttt acgcagccgg taacgtcgaa ttccgacatc tccatactgc cctggctgta
     3721 tcctacatac tgctcgttga cccatacatt aaaagcactc atgaccccgt caaagcgcaa
     3781 gaatattttt tttcccgacc aggcggcagg caactcaaaa gtacgcctgt aggaacccgt
     3841 cggagtcctt tcgctaaaag aagtataccg gggatcgggc tctgagctta ccaacggcgg
     3901 ttcgattttg aacgaataac cggcactgat gtaaatcgga gttccgtaac catgcaactc
     3961 ccagttggag ggtaccggga tttccgccca gccggcatcg tcgtaatcgt tccggtaaaa
     4021 ggacgttggg gcttcttcta ccttggggac gtattgaaat ttccagttgc cgttgaggct
     4081 catcgttctg tcctgctttt cctccaaata aggaacgtaa gaagctctgg caggcagtct
     4141 gtttatttcc aatactctat gattctccca gagggggcgg aatgcctgag cactacacag
     4201 gcaagaaccg attataaggc cggcgatgag caggccgatg tttttcttca taatgaatgt
     4261 attaacgatc caagggcatc caaactgtca tctcttcaaa acccctgttt ccgaaagcat
     4321 aataaggaat caatcgtacc ggcactttct ttttggccga agacacttct ctgtaaagta
     4381 cattatccca gttttgttgg tccacatacc aggcttctcc cttgaggctc atcaggcggc
     4441 tgccgtcgat ttccatcatc tcgggttgaa agctaatgtc cgaaggaatg ctgatattat
     4501 cgattttata gttccccaga tcagcagact ccaggcaata caccagaggt ccccacataa
     4561 cggctgtctg attgcgggtt tcttcgagca agggattgga ttcaatcaat ctgggctgaa
     4621 tacgcatgtt gaatgttata ttatcggtct ttttccattt tgcctgtacc cgggcatagc
     4681 ccttttctac ctgtaattct accggattac cgttaatgct gagttcgtaa tgatcgcacc
     4741 atccgggtat tctaaaaaac aggctgaaat cggccttgcg gggaagcttg gcaaacgtca
     4801 gctccacttt gccgtcccaa ggataatcgg aacgctgaac gatctcaata tcggaaccat
     4861 ccgccaattt ggtggccaag ttactggatc catagaggtt tagccatact ccctctttgg
     4921 aaaggctgta agcataattc tgagcttcac aaatggtacg taccgtattg ggcggacaac
     4981 aaaaacaact gatgtattct gtacgctctt tgggccaacg cagcacatag ggaaaatctt
     5041 ttgaaagacg cagaggattg gtatagaaat acttggtccc gcctaagctg atacccgaca
     5101 agacactgtt gtacaaagtc cgttccaata catccacgta tttggcatct cctcccagca
     5161 gaaacattct ccagttgaaa aacatatttc caatattggc gcaggtttcg ttatgggcgg
     5221 tagaattggg taattggtaa gccctgccgt aggactgatg tactttttgt atggaatcgg
     5281 gcgtatagtt ggtcccatcg ggcgaagtgc cgtcgtacaa agctccgcaa ccgccggtaa
     5341 tgtacatttt acggtaaacg atatccgacc agatcgactc gagattttcc aacaataaag
     5401 cttcgccggt ttcggcatag acatccgcca ctccggcgta taaataattt gccctgacgg
     5461 catgccccat agccagtttt tgctgacgga aaggcactct gtcctgattg tcgtcggttc
     5521 cgttttcgac catacccctg atattaatca ggtttttaga taagtccagg tatttgggat
     5581 cgcctgtggt acggtacatt tcgactacgc ccatataatg agagggacaa atggcgttgc
     5641 gcgccaattc ggcggtggct gtttcgtaga aacggcacaa aaaatcggcg gccttgatgg
     5701 caagttccag catattgcgc ttaccggtag ccctgtaatg cacgcaggca gcagtcatca
     5761 ggtgaccatg attgtaggtt tcaaaattca acctgtcgct gaaagctcct ccctgggcag
     5821 tcccaacagc agttcctacc ggtccgcttt gttgcgaagc ttgttctttt tgtcttttac
     5881 gctcgggaat gatcacctga gtatgcatgt agccgtcttc gcgctgggct ttggcaatgg
     5941 tggtaatgat tctgtccatc aaagcatcca gtttgggatc tttggtgacg gcatacactg
     6001 ctgccgcact ttcgaaccac ttgaaaaaat ctccgtcgtt aaagggaggg ccggaatgcc
     6061 ggccttcggc ctcgccggca gcaatttcca gattcctgaa agcatgcgaa acttctggat
     6121 cgctcatcac atgccacatg ttaattacca tagtatcgcg gcaaacctca aagcgttctc
     6181 cccaaaaacc atctgtccaa cttacagccg acacatcggt gttccatatt cttgcataag
     6241 ggcttttcgt ggtgtttacc aatcctttgt cctgtgcata caggccggtg catgccagca
     6301 gggcacacag cataatcgtc tttttcatgc ttttcttcta ttataattgt acgttaaaat
     6361 gatattctcc ggagcccaat tcaatcaatg tgccattgtt ttcggttccc agataacgaa
     6421 tagtccggga tgaaccgatg ggccggtcgc tttctttaat attctttttg cgaacaacag
     6481 gcaaataaac cagagccgaa gaattggccg gtactttgat actccagtct aaagatccct
     6541 ccgatttttt ccagttcgaa accaccaatc cgtagggggt tttataggag gcattgataa
     6601 agttcaaatc ggcaattcca aaatcgggtt tcattactat tcgcttgtaa gccacctgtt
     6661 cggtatcgct gctgatgcct gccaggtttt caaaacacca aatcagcagg tcgcccagca
     6721 acataacatg gttacccgaa ttcatgctgg gagcggccgt gtcgccattc cacaattccc
     6781 aaatggtagt ggcgcctttg gtcagcatat agccccaact gggttgtttg gtagtgctgg
     6841 ccagcaagaa ggcaatgtct cccctaccca tggcagtgag ttcacgcatc agccatgaac
     6901 cgccgatgac accggtagcc atggtgcctc cgtaggtttc caacagtgtt ttctgaacat
     6961 tttcttccac acccttccgg gcttcggcgg gaaccagatc aaaagccaaa ggcaggagat
     7021 tggctgtaac cgtattgttg ccgtagtact gtttttcgct gttgtaaaaa tgccggttaa
     7081 aagctgtctt catgtcctgg gccataatca gataaaccat ggaatcttcc ggcttattca
     7141 gtaaattggc gaaaccggac attatatgat acagcttgta aaaataagcg gtcgatatca
     7201 gtgcgccttc ggtaatgcgc gaaggatcct tggagtgtat gatttcggga gcttcgggag
     7261 gcatacacca atcaccgtat ttgtcggcga ccaaaagacc gtctttgaga taatgccgac
     7321 gcatgtgctc cataaacagc ttcatagatt cgtaattctt ttctatgggc ctcaaatctc
     7381 caaattggcg ataaagcatc tcggaaataa aaaagaaagc ggccggccaa gtgacgtcgt
     7441 cggaatagta attccaatac gccggagcca catccggaat gctgccgtcc caacgttgag
     7501 cttcgcggat atcgtccatc catttggcgt aaagtttgtc gttttcaaac aggaaacttt
     7561 ctccgtaagc accgatggta cgatctccca gccagggcat acgttcattg cgctggggac
     7621 agtcgatggg catacctttg taatttccct tgatgcccca gtaagcattg cggacaatgc
     7681 cgttgagcac ttcggacgaa gattcaaaat accccaggtc ttccatttcg tcgctgacga
     7741 actcacctat aaaattttcg ggcttgggct ctccgggata attgctcaat tctacgtaac
     7801 ggaaaccatg ggtcgtaaaa atcggagccc attcctcgcc ctgaggatcg cctttcagta
     7861 tatatttatc cgtcaccagc gtctctcaaa ttggccatgc tcaaactacc gtcttcctgc
     7921 aaaatttcgg caaaacgcag tttcacctcc tgtccggcat tgccttttac cttcatacgt
     7981 atccagccgg tcatgttttg acccatatcc aggatgtagg tgtttttatt aatcttagta
     8041 atagactgtg gatgaaagac atcgcttact ttcatttcgg gattgggctg agcgcgtatc
     8101 acagccgtag gaatagtcac ccgttgtgcc ggaaaccaat ttctgccggt tttgaatccg
     8161 acctcagtcc aaccgggaaa ttccttgcgg gcatcgtatt cttcaccatc gaattcgttg
     8221 ttactgataa tggggccgtc ggctgtcatc atccagcgtt cgttgctgcc tatcgtctgt
     8281 ctggtaccgt cttcatactc tatgtttaga atcattctca gtttgggata accgaaattg
     8341 gcccatttgt agggcttgta attctggcgc atagcataat agcgaccgtt tcccacaatc
     8401 actcccaaag cgttctgacc ttgttgcaag gaagacgtca catcgtagct gttgtaaagt
     8461 atgcttctgc ggtaatccgt gggggccgga gtcattacat gatttccgat gcgtccgccg
     8521 ttgatccaca actcgtacaa gcctagtccg gaaatatgta ccagtgcctg acgcaccggc
     8581 ttggcgcaat taaattcatt gcgataataa cgggccgaaa gccgggcatg cgctttttcc
     8641 acgtcccagg gcataggccc gtccaagcct atccagcgac tgtttttcca gtcgttttcg
     8701 gtgaaaagac cggtagccca tctggccgtt tcactttcta cagcctcctt gttcgcattg
     8761 gtccaaatct gcactttcca gtaatagtaa cggctacttc gcaattcggg gccggcatag
     8821 ggaatcaaaa tggaagcatc tgaattttgc ttacctgaat cccatacgtc tgcctgacct
     8881 acagccagtt tttcctcgct cgaagccacc ataagcctgt atgccgtttg tctgacccca
     8941 cgggcctctg tttgcaattt ccaactcaaa cggggattgg gcgcatcgat accaaccggg
     9001 tccttgagca tttcacagct caattccact gctttgaggt cgggagcgga agactggtct
     9061 tgtttgcaag atataaacac caggcttacc agcgctgata taagaataag tcttttcata
     9121 gcttttattt taaatcgggt aatacggtcc aactcttatc tacatatttt tttgcggcat
     9181 ctacggcaat caatacaatg tcgttgcctt cggcctgcgg tttcggagat tgaaaaagct
     9241 gaacgccgtt ttcaaattca cccaggtaac tgagagcccc gcttgtggga tccatgtacc
     9301 agaccgactt ggtctggcca cttattttgg ttaaatctat ttccatggga cgacttgtat
     9361 aattgtaaac caacagataa tcgttaccac gggtagcgct gaggcgttca taacgaactc
     9421 cgttttcacc ggctatgacc gactgatcgg gaatgcgttc aaaaaaggga aagctcaaaa
     9481 tcaggttttt caaatacttc atttgattaa aaccctcgtc tttaagcgct tccacccaat
     9541 gtaccgtagc accgtaagcg ccggggcgcg gacctccttt gtacatctgc atgatggaac
     9601 tgttgccgta agtatggccg aaagaaccgg caaatacgga ccaataggcg taacgacgaa
     9661 catccttggc ctgccagaga ggttctttcg gatcgtgcag cccctggggt ataccctcgt
     9721 agctgggttc gccgtctatc accggcttaa gcggctcgta ggtcttgctg atttcaacgt
     9781 agcgccagtt gtcttcttcg gttgaatcgg gcaaaaattc cttacccggc tccgattgtt
     9841 cgtagcgccg gtgcccactt tgaaacatat tgaaatccag ccattcggca ttgtgcatcc
     9901 aaatggccga taaggtgcgg ccaaagggat gaaaagtcat caagtgattc ttgtcgatgg
     9961 ctttgatgct ttcggctaaa gcatgccaaa cttcgggttt aacatcgccg cgaatatccc
    10021 ctccgataaa ccagataata ttgggacgat ccttgtagcg ttctgccaga aaagtgccgt
    10081 aagctctggc ttgttcttcg tccatcagac ctcttttgac taaacctccc caaatacata
    10141 ccataccgat gtatattccc ttggattctg cggtttcgac gatgtaatcc atgtgatccc
    10201 aatagccgta tacgccttcc tgattgatct tgctgaaatc aaatccgtct atcatggaaa
    10261 gctgaccgta ggtattgacc gcaggcaccc cgttgatggt ttgcacctga accacgttgt
    10321 aacccgcctg acggcagttc tcgagataaa actccgcttc gagccggttg agtctttcgg
    10381 gtaacaacca tccggtttca cccaaccaaa aaaatggttt gccgttttcg tgctgtaaat
    10441 agcattgagt agcgtccacc tgcaatttcc cgttatccca gggcaactct tctttttgtc
    10501 cgcaggccag aagcacaagc gtcagcacca acaggctgtt ctttaaaatt aaattcttca
    10561 gtttcatttc tactttagct taaggtaatt gttttataaa ttggtgtttt ccactttgta
    10621 taagctccgg catatcctcg ccgggtaaaa acaccttggc atcgcaatta gtcgggatct
    10681 ccaccttcat tgcgaactgt ttatcttcgt cgagttccca ctcgacgccg atcgtaccgt
    10741 aaagattttg tgtacggcat ttcacccggg tcagatcacc cacaacctcg ggttttatta
    10801 tcaggtgctg agctccgggg gcttgttcgt gatcaaactg tatgccgccc aatgaacgga
    10861 aaaaccattc atcgatcgaa ccgagcatga aatgattcca ggaagcacct tgtcttggat
    10921 cccattgttc ggtcagagta gtggctccga attttacctg aaaaccgtat cccggcgttt
    10981 cttcgtgatt gtgcattttg tacatcagct cgttgaggcc gttgcgggcc aaaacctgaa
    11041 acatataacg attgccgact tcgcctgtgg tcagcctgtt tccgtgagct tcgatatcgg
    11101 ccaccaaacg atcgagtaca cgctgacgat actgtggttc taccatatct aaaaacagcg
    11161 gcaaggcata ggaagtctgg ctgccggtgc cgtaaataca actgtctgcc ctgaaaaagc
    11221 gttcgtgaaa agctttgttg acttcattac caagttgggt ataatagtct atgtctcccc
    11281 ggattcccag catcgtggca gcacgaatca acaattgaat gtcgagatac aaatgcgccg
    11341 taccaaccag agggaccggg gtgttgcgcg aaaagcccga tttgaaatca ccgtagtcgt
    11401 accagtctcc caaaccgtgc atcactatgt gattgtccga cctgctttcg aggtaatcga
    11461 cgtatttctt catgacaggg taatattccc tgatcaggcg atcgtcgccg taacgccaat
    11521 aatacaggaa gggtagtatt accgaggtac tgccccactc cggcgaatcg ttccacaatt
    11581 ccgaaaactc aacgtacatg ggagatgtgg tgggtaccaa tccattggga tattgttcat
    11641 cggccatatc cctcatgact tttgggaaaa gccggctcaa atcgtaatta tacatcaggc
    11701 cttggccgca gagttgtacc tgttcgagcc agcctaattt ttctctgtgc ggacaatcgg
    11761 taaagaccga ctgcatattg cttctcacag ccatattaac aagtttatgt gcattgttga
    11821 atatggtgtt ggacgactcg aaatgcccga tattttccgc tgaattatgt acaaaacagg
    11881 aatttatttc gttaataacc ggcaaattat ccggattctc accgccccgc attactgctc
    11941 cctccacctg tatataacgg aaaccataat aagagaaacg ggggcgccag tattcttccc
    12001 cggctccttt gagggtgtat tcataataat gcggcccgcc cgactggctt tggcttacca
    12061 acccacgatc gttgagcgat tcaccaagag ttagtttgac tttatctcct tttctgcctt
    12121 tcaccttgat ttccggataa ccggaaaggt tttgagccat atccaacaca tacacccaaa
    12181 cagtatctcc tttttccgcc tgagcctcgc cggaatttct gtttcgtgcg ggcggcatgg
    12241 ccaaacgctg catgctcttg accggataac gatgcataat tttaaccggg ttggctgtct
    12301 gggccaacaa tacgccctta ggagcctcct gcaccaccac cgatctccag ttgctatcgt
    12361 caaaaccggg tttatcccag ccggtctgtt ccaaattggc atcgtaatct tcgcccccat
    12421 aaatacaatt gaaggtaatg ggactgaaat cgtatttcca gttttgatcc gagatccatt
    12481 cgccggtgct tccatcggta taccgaacga agagtttgaa aaacaaggta ggaggaccaa
    12541 aactcacgag caatttgcga taacgacctc cctgaacatt gaagaaacca ttgcccagca
    12601 atacaccggc tacgttttcg ccttttttca gcaagtcttt gacatcgtac acattgtaat
    12661 acaccgtttt gtcgtaatcg gtccataggg gagcaaattc agaatcaccc accttttgtc
    12721 cgttcagact caattcgtaa tgcccaagcc cactgatata caccagggct tcttctatgg
    12781 gtttacccag attcagtgcc cggcgcaaat aaaggctttg tttagacaga gggtctgtag
    12841 ccgcccacaa agccttactt tcgggatcac gattaactaa attaccttca tattggcggc
    12901 cttcgggcaa tttggaatca gccctgctta tggctcctat ccattgggca tccagccagg
    12961 cttcttcggg tttaatccaa aacacctgct gtttactcca ggccgttgta cgaccctgtt
    13021 gatcccaaat gcgtactctc catgcgtatt tttggcgtgc ttgcaattcc gggccttcgt
    13081 aaagaacctg ctgactgttg ttttcagtga ctttcccgct gctccatagc aaggattttt
    13141 gagaggcttc ccatcgataa aattccagtt cgtaggcttc ttgcaccact gaattccccg
    13201 aagcaagaat ttgccaaccc aggcgaaaat ctttgttttg caacgccaaa ggcccttcct
    13261 gataatccag gctcatttta ccgatccgga tttccggtgt acaggaagaa aggctgccgg
    13321 caagcagtaa aagacccaat aaaagttgaa atcggcttat atagcttctc gttttaacaa
    13381 aacgaatcga tttggagtgc ctgtttttca ttataaagta ttgaattatt gattaacact
    13441 aattttattc aaggcctgtt ctctgagcta aaacgatttt ggaacagcac agaccagata
    13501 acacaacatg ctgaaaaaca acgagataac agaaaaatgc tgttgtattc gctgcctgag
    13561 ccaaaagtat taaaaaataa tctgaaaaat gattagattt aggctcttaa ataacaattt
    13621 atcctaaatt gcgagaggca acataaataa gccggaaaga agtaaaacct aacactttat
    13681 tcattatgat tgattctaaa attgaagaaa gactgcagga gatgagggct ttctaccgta
    13741 ctaacactac caaagattac cgttaccgga ttcattcact caaaaccctc tcttcgtcca
    13801 tagccaaaca cgaaaacgaa ttgatggagg cattggccgc agatttgcat aaatcgcatt
    13861 ttgaatcgtt tgccacggag atcagcatag tacaacagga aattcgtttt cagatcaggc
    13921 acttaagaaa atggtcccga accaggagat tggctacccc tatggtcttt ttcccttcca
    13981 gaagttacct gtacaaagaa ccttacggag tggtacttat catagcttcc tggaattatc
    14041 ctttccattt gttgttcaat ctcctggtcg gagccctggc agccggtaat tgcgccgtgc
    14101 tcaagacttc gcccctggct ccggctactg ccggagtgat ggaaaaaatc gtatcctgct
    14161 ttcccgtgga ttatatcaac atttggcacg gacacaggga ggtgaatcag gccttgctca
    14221 aagcccgttt cgattatatc ttttataccg gcagtccggc cgttggtaaa gtggtaatgg
    14281 aagccgcctc caagcatctg acccctgtca ccctggagtt gggaggcaag agtccctgta
    14341 ttgtggacaa agacgcaaac atccgttttg ccgccagaag aatagcctgg ggcaaaacca
    14401 tcaacgccgg tctaacctgt atagcacctg attatctgtt ggtgcaccat agcgtaaaag
    14461 aagagttttt attagagtac aaacaagcca tcttttccat gtttggcagc gacccacaag
    14521 cttcgcccga ttatccgcgc atcgtatcct cttcggcact ggcacgtctg agcggctatc
    14581 ttaaagaggg taaaattgta ttaggcgggg cgatagatcc ggaagattta tacttggaac
    14641 ctaccgtatt ggaacaggca aaaccggaag ccggcgtatg gcaggaggaa atattcgggc
    14701 ctgtccttcc actcaaagaa tttgatcaaa tcgaagaagt gctgcaattc atttacaaga
    14761 gggaaaaacc gctggccctg tattatttca gcagaaacaa ggaaaacatc cgcctgattg
    14821 taaaatcaac ctcttcggga ggtgtttgca tcaacgacac tttgttgcac gttgccaatc
    14881 accatctgcc cttcggggga gtcggccaca gcggaatggg acagtatcac ggcaagtata
    14941 gttttgaaac ctttagccga aataaagcca tcgtttgctc cagttcgaat atagatattc
    15001 cgctgaaata cccgccttac agggctaaac taaaacttct cagaaaactg ttttaatacc
    15061 ggtcttcggt ttcgagtaag cctttgggta caaattcaat atagtcctaa ccgaaatagt
    15121 cgctggtatt gctcgacaaa acagatttca tacggaaagt atgtgttttg ccgtctccta
    15181 aagtataaat accaagtatc ctgcgcaggg agttccaatc gtcacgaccg gacctgccag
    15241 agggatacat gcaaaaatac tccggagctt tcatccagcc gcggttgcgt aaatccttgt
    15301 cgttgatggc cccgttgtct tcggtgtcta tatcggccac ccagcccaca tcgggatggt
    15361 gagcgttttt acccatatcg agcggtatgc cgttgggaat accgtctaca tagatctgaa
    15421 caatattctt ttcgcgggtc ttgataccaa tgcgaatctc ccaggagccc tcgggcaccg
    15481 gcggtaaaat atgagtaaag tcgtaccaac cgtaaatatg aaaacggtcg ccctgcaaac
    15541 taactgcgcc tccggtggga cgcttgccgt ttaccttggt ttcatcgtct ttgtagatca
    15601 gatttttgaa aaaaccggaa ggatagtaat actctctttc agcacttact ccgcgcgccc
    15661 tgatattcat catctcgggt aaggtgctgt acacatccat gcgtaaacgc ttgttcaata
    15721 catcgttgat aacagttccg tcgtaaagca atattttatc gatgggatga aatattccat
    15781 ttacacaagc tctgtgcttc attcctttgc ccaagattct gatgtgctcc ccgttgcttt
    15841 gcacattgaa acggttgccg gcacgtacct ccagcagcgt attgggacac atagtctgaa
    15901 tgtactccac tccctcttta ccttccatca tgcctccgtg attgtaataa tagtctacaa
    15961 actcgttata ataacacatg taatcaaaca aatgatagga aacaaagcgg ttcaagctgt
    16021 tcttgcgatg agtaaagtca ccggcataaa cggcatcttc agggaaaacc tcgtcgtaaa
    16081 cggaagcagc aaaagcaatc aaatcgtcca aattgtaaat accatactca gcataaatcg
    16141 aatcgggttc aactaaggct gtgtatccgt acaaacggtc ggggggagtc gctaccgtag
    16201 ctccggcagc attcaaaaaa ctaatgggtt taacgtaaga atcgtccctg attcccaaca
    16261 aagaatcgct catgcaggtc aggtacaaag cctcggcaaa aatcgagaaa gcagaatcgg
    16321 aacgaatgga ttcgggcaat agataggttg aaggaaccaa gactttgtca ataacatgaa
    16381 tcacaccatt atgtacttcc tggtctttca acttaatggt agaactggag tttaccaaaa
    16441 taccaccttc ttcggcatac ttttcgaaag aaatgtcgat aaaacgcgac atcatattct
    16501 tgttactcaa acgcccctgg ggaaaatcgg tggataagta ctttttggtg tcaataatct
    16561 ggtaataaac catttcgcgc aaaacgtcca cggaaaactc ttcaaaacca ctcttggaag
    16621 agtcggcata ataagcacgc atggcgtcgt tggtgggaat aaagcaggt
//
LOCUS       DAOSGL010000011        16523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_2856_length_16523_cov_4.366165, whole genome shotgun sequence.
ACCESSION   DAOSGL010000011 DAOSGL010000000
VERSION     DAOSGL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16523
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_16523_cov_4.366165"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PKY68_00785"
     CDS             complement(<1..422)
                     /locus_tag="PKY68_00785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH23609.1"
                     /translation="MSGIAILLTTCNSGKYLRPQLDSLFAQTMRDFEIHVHDDGSKDD
                     TLQILAEYQKKHPELILREDPQPGRLAKGSFTWLLENTQADYYMFCDHDDVWLNDKIE
                     KTYSKMRACEEAQPGKAVVINTDLKVVDEQLNEIHPSF"
     gene            complement(410..2236)
                     /locus_tag="PKY68_00790"
     CDS             complement(410..2236)
                     /locus_tag="PKY68_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOH23610.1"
                     /translation="MPKITAFTGKFNYYFALLLAFYLSLQDRWLILVFFCWLVSWIID
                     VILSKRYLEFRLDKQHWTVYILWVSALLMLISVFYSTNLEYAYKVIERRVVIAAVPLM
                     LSIGFSKDYKLKPLLVAFVAGNVFSLLFSLGIPIIEYLSDFRSRNMIHKFPWKAFLEF
                     FLLFKHRTYFGINQMLALLSLLYLRKDLIPKMGKYCYWQCFIVYALVCTSLMLVLGGR
                     IALIILVLVASFYVYNALNKHQHRKFALAVILLGVSSMIALYVYHPRFESIRPGKLTE
                     SSSLQSKDPRLGIWYSGMQVLSENNRFLFGAGIGDVKDLLKEKYEQNRLSPEVIQGNS
                     HVHNTYLQALLETGIPGLLCLLILLIAFPLSLRDKNERLYAGTIAFMFAMLMMVEVVF
                     LQIGGLIVFCLSFLFLSKAGGRKEEHLSLPAGFCKTFTIINAAMVLMALVVFCYPLYG
                     KQGYDPKDPATYATDNYELIKDLPGDPPGELQDCWGYKISSTCRGYYEPHFDRMEFLT
                     AFYQPSQGKGRFSAWCYVSEDFDGVHVQIFTSPAHTGQGFSDEYDLSRKGSWQKLSFD
                     YSGVDKLYPMSLFFAKYRVKDFTTLKGYVVFALPVFELTCQE"
     gene            complement(2245..3270)
                     /locus_tag="PKY68_00795"
     CDS             complement(2245..3270)
                     /locus_tag="PKY68_00795"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HOH23611.1"
                     /translation="MKKNRQWLLLAMTLFLIAFAGFRGNDVSKDHSIFQAFYDKALPF
                     QTLFSNPGAFFKAAKVEPTFLILASFIKTYLPGWGFTFLMVLFAFLSVTLKVKAIVKY
                     SEFIFLSLFIYLCNTYMLHEIIQVRAGLAAALAMLAFEPLVERKPWKYLLIMLLAILV
                     HYSAIIFLPFYFLNTKKINPVVYIAVIVVPFLLSVFHFDPISLLLKYDLGVYSEKLSI
                     YITEQLRLKHAINKINLTIIFQLILSVFLLFYRTDLLEKNKYSVLFLKINMISVGVFY
                     LFFGLPVFAFRLWEMLNVVQIFLIPTLIYYIKPRWIPELIIVLVGIALYYNLVLQGHI
                     REYSLIF"
     gene            4171..4638
                     /locus_tag="PKY68_00800"
     CDS             4171..4638
                     /locus_tag="PKY68_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23612.1"
                     /translation="MKRILLLIVLLTVFVCLSSTRAQTIYLRSGQKIPIEGNGLHSIV
                     PLENGYAMAFKGKDGQIMHVFHFDDLSSTKWTGTSDAVETLSSDKQMIVYLAEKKELI
                     IRNGVEDGDICVFSLDGSLVARGKGRSLDISTMADGLYIVSYNKVFNAKFLKK"
     gene            4635..6887
                     /locus_tag="PKY68_00805"
     CDS             4635..6887
                     /locus_tag="PKY68_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH23613.1"
                     /translation="MKKNVLTTFAASFLIIASNLAQDTIIKTIDVFRNNTASVSYLLD
                     SIKRIDFVKVPYTKTDFYAGTLNLTASAGIHSKFHVQSNIKWDVVTDAEWVKGFIRYD
                     ENLYAKENGYCYIVTTAHTGQQNRKASLSVINSDNQCSKTITIIQKPYELLFLNSNDT
                     EYSLDIGCTDSSFNLYFSPNFDWQLTYKPSWIKHNIVAPQVQGNESTDTLFSREISVD
                     DAQSFTVGQNVPWEDRQGRVVIEGHGQEIVAHITQKGLNSPYEDMYTILGSPNSCRLY
                     TIDDFGFIMNAISNDLEAADAIMPDNVYNWFGVCGDLTSRTGGYRNPFIRYQSCYNVI
                     DLANIILRQDDPNLDPAFRAKIGEAYVIRAFAYMNLAPYYQFGYEALGAKVDSSLCVP
                     LVNPGIDPADNPRATVTEVYQQIISDLTTAIYKLDGWQRTNKSKIDQQVAYGLRARAY
                     LTMGEWAKAAEDAEKAMRGFQCATREEVSKPFLYDIDEHNWIWGYDMTEEMANSNPYA
                     TSSSLLRSFSANAYSTGLQIYCYINNILYDKISKTDVRKGWWVNDSLYSPLLDSLTWD
                     GLKGQEIATDTILKVKEPYLPYTNVKFGCNTCGTHTNDEDWCWMRAEEMLLIQVEGLA
                     RSGCDSIASSILSAFVKNNRDPEYNISNSGRSLLDEIWFQRRVELWGEGFSNADTRRL
                     NKPLVRFTDASTSNVPENFRFNMEARDSWWLMRFPEKVLNNNPGIVDNTEGNLPILNQ
                     NPELRDGVTD"
     gene            6904..7632
                     /locus_tag="PKY68_00810"
     CDS             6904..7632
                     /locus_tag="PKY68_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23614.1"
                     /translation="MNKSLRKILLTAIFLSIASSLLKSQNYICIVQEGKTDSINAEII
                     DSIVITEWKSLGIGQFYDQFVLNHVQPVEILKSEEEDKYRVLNPYQDLSAEEWGEWMG
                     GTDCPYIEFWSYDSNNSTYLKWDKFWYSTLLYNNTGIDLKAYYPSFLSSSFAYLDLKS
                     TWFMENVAVLYPYWFVDGLGGYGAGDAVYISLPGGTDLVELFFDSSTRSAPAKQAVQV
                     KDTLPPSHSTHTKNEIMKYNATHQ"
     gene            complement(7765..7837)
                     /locus_tag="PKY68_00815"
     tRNA            complement(7765..7837)
                     /locus_tag="PKY68_00815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7802..7804),aa:Lys,seq:ctt)
     gene            complement(7910..9823)
                     /locus_tag="PKY68_00820"
     CDS             complement(7910..9823)
                     /locus_tag="PKY68_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOH23615.1"
                     /translation="MSKLQPFVQIENLSKSFGDLVLFHKISFGIAQQQRVALIAQNGK
                     GKSTLLDILAGNKDYEEGSVVFRRGLKIAYLKQTPEFPVGLTVFQACFQAANEVTETI
                     ARYELKMQEIAGKSMDAEAEELLHLMDHYKAWDYEVRIKQILSKLKIPNFTQKVGQLS
                     GGELKRLALANVLITEPDLLILDEPTNHLDLDMIEWLENYLTKSGITLLMVTHDRYFL
                     DRVCNTILELEDKQLYCYNGNYAYYLEKRRERLEISNAEWERAQNLYRKELDWMRRMP
                     QARGTKAKARSDAFYELEERVSQRHKTEQLRLGVKASYLGSKVLEAKYISKSYGELKL
                     LEDFYYQFAPFEKVGVVGANGSGKTTFLRLLMGEEPCDRGSIDIGSTVRFGYFSQEGL
                     SFDENKKVIDVVRDIAEEISLGNGKKLSASQFLTHFLFSPEVQHNYASKLSGGEKRRL
                     YLCTVLMTNPNFLVLDEPTNDLDILSLNILEEYLVSFKGCVLVVSHDRFFMDKIVDHL
                     LVFEGDAIVRDFPGNYTQYREWKEQQEALLRKEKESERKSKTNLPDIEPKKEESSSNR
                     KRTYREEQEFIALEKEIAQIEENIALIENDLASGQLEGGAIEQKCIELSRLNQELDKK
                     ALRWMELGELEKK"
     gene            complement(9820..12627)
                     /gene="uvrA"
                     /locus_tag="PKY68_00825"
     CDS             complement(9820..12627)
                     /gene="uvrA"
                     /locus_tag="PKY68_00825"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOH23616.1"
                     /translation="MDNNFVDGAREHNLKNIDVQIPRNSLTVITGLSGSGKSSLAFDT
                     LFAEGQRRYIETFSAYARNFLGNRERPDVDKITGLSPVISIEQKTTNKNPRSTVGTQT
                     EVYDYLRLLYARAGEAYSYLSGEKMVKYTEEQVLELILEKYNNKAIYVLAPVVRSRKG
                     HYKELFETLRKKGYLNVRVDGELQEIIPGMRLDRYKNHDVEVLVDKLIVSPNDRDRLK
                     KSLQVAFKQGKGLIMIYDKDQDELRHYSSQLMCPVTGLSYGDPAPHSFSFNSPQGACP
                     RCKGLGIINQIDFNKIIPDDSKSIFEGGIVPLGKYRNSMLFWQIEALGEKHGFKLKTP
                     IRDIAPDAMDEILNGCEERLPIKNTTLGLYNYNVHYEGVVKYIQLQQDDDSPSDAQKW
                     AEQFISTAVCPECQGLRLKKEALHYRLAGKNIAELAAMDIKELALWIKDLDRHLNDKQ
                     KRIASEILKEISQRLQFLLDVGLDYLSLNRPSAGLSGGESQRIRLATQIGSQLVNVLY
                     ILDEPSIGLHHRDNRRLIDSLKTLRDKGNSIIVVEHDKEMMLASDYILDLGPRAGRLG
                     GHLSFAGSPKDLLQASTLTSDYLNGKLRIKAPTAYRPGNGRVLSLKNCRGNNLKQVNV
                     DFPLGKLIVVTGVSGSGKSSLINHTLRAALSKHFYRSHQEPLPYDSIEGLEFIDKVVD
                     VDQSPLGRNPRSNPSTYCGVFSDIRNLFVNLPESKIRAYKPGRFSFNVAGGRCETCKG
                     NGYRAIEMNFLPEVFVPCESCRGKRYNRETLEIRFKGKSIADVLDMTINMAVDFFENQ
                     PQILHKIKTIQEVGLGYIKLGQPSNTLSGGESQRVKLAAELSKRDTGNTLYILDEPTT
                     GLHFDDIRVLMQVVNRLVDKGNTVIIIEHNPDVIKMADHIIDMGPEGGKEGGRILFSG
                     TPEELALSRQSYTAACLKDLL"
     gene            complement(12660..13601)
                     /locus_tag="PKY68_00830"
     CDS             complement(12660..13601)
                     /locus_tag="PKY68_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOH23617.1"
                     /translation="MMKLNKYIIAVVFLMYNGFFSMAQSIYPQALIHPIDLPLRTEFC
                     GERIDLRRWDLRERFDRELLAMNYMHSSSIQLIKKANRIFPLIEPILKANQVPDDFKY
                     LACIESNLNSGALSPAGAAGIWQFMPETAREYGLQVDDQVDERYHLEKSTVAACKYLK
                     DAYRKFGNWSTAAAAYNGGINRIANELEKQGVDNALDLYLNEETSRYVFRIMAAKDFM
                     ENPAKYGFIFSAEYLYMPLQYNYVSIHKSVGDWVEFAKEHGLTYLQFKEANPWLRGSR
                     LNNPRGKIYNIAIPMESSQYFDVKNLYIHQKNWIRNK"
     gene            complement(13598..14926)
                     /locus_tag="PKY68_00835"
     CDS             complement(13598..14926)
                     /locus_tag="PKY68_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH23618.1"
                     /translation="MHRKRSLKYLVFNLFFLFFILSLPANAAFTASGGSNGRPYEYIP
                     TASTGLEKVYIFDGLQQAELRFNTADPSNWTWYRYQTGLEDAQAVPLTDISLESDATV
                     LKNPGAYGYYVSQGDQIKAVYIIDYSAHKLDYTKLEGVENAEDVCSNLQLVVEGIQED
                     LSYYALSGSPARRTVDRIHSLTWTDLEWDEEKEQYLPVDKNSNKEGFLSGFIVDAPLQ
                     NTRFKLEGDQFAAHFGLSAKSIETSYQAVRVSTHAKAVPEEREQADNEIGAGSAGSLT
                     GSAPFKLDFYSNVNPATRYTEWYIYTNPGSKEEYLRRTETDINYTFNQAGSFEVKLLA
                     SNNICKDSATFTVRVTESSLDCPNFFSPRSTPGDNDEFKVAYKSLIKFHGRILNRWGN
                     VLFEWTDPSLGWDGTFKGKPVSPGVYFYIISATGSDGIEYRKKGDINLLE"
     gene            15166..>16523
                     /locus_tag="PKY68_00840"
     CDS             15166..>16523
                     /locus_tag="PKY68_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOH23619.1"
                     /translation="MKLRPIIALCTCLVFFTLAVKAQENNIPVVKLFDKEYYKYEVQA
                     SETLYGLSRRFNVSQEEIIAMNPFLIEGLKTGQILLIPVKIFEPDKDELPEKTPDNSA
                     EKSQEKTPGKSCGKSSQKSLKPNEDSLSAASDSSVLFEKWEYEYYTVEKWRENLSDIA
                     DNFSVDIDEIRLHNPNVPDRLPRGSLLMIPIKKIEVVSEIKTAEEEPSEDLEPKIFRK
                     PNIALLLPFMLDDTTANSIERYVEFYEGVLMAADSLKQLNFSFELSVFDTGNTLESLR
                     KVLTSGWLDRANYVIAAVNNEQMMFLSDWSVREKKYLILPFSSRIPETEFNPYIYQIN
                     PPHSLNHQALLELDTSYYAGKNILFIRTGNESSDERRELFDTFQISLKHYGIPYIEIT
                     DNEPPAHIAYSFPDTIARHLSLDKENLIVPAPFPMTESNRVITMIGSAANIKSKARIT
                     LLG"
BASE COUNT         4721 a         3663 c         3550 g         4589 t
ORIGIN      
        1 caaaaagaag gatggatttc gttgagttgt tcatccacca ccttcagatc ggtattaatc
       61 accacggctt taccgggctg agcttcttcg catgcccgca ttttggagta ggttttctcg
      121 attttatcgt tcaaccacac atcgtcgtgg tcgcaaaaca tgtagtaatc ggcctgcgta
      181 ttttccaata gccaggtaaa actgcctttg gccagtctgc cgggctgtgg atcctcgcgc
      241 agtatcaatt cgggatgttt tttttggtat tcggccagga tttgcagggt atcgtctttg
      301 gaaccatcgt cgtgcacgtg gatttcaaaa tcccgcatgg tctgggcaaa cagcgaatcc
      361 aactgaggac ggagatattt cccgctgtta caggtggtaa gtagtatggc tattcctgac
      421 atgttaattc aaaaaccggt aatgcaaaaa cgacatagcc tttcagtgtg gtaaaatctt
      481 taaccctgta cttggcaaaa aacaacgaca tggggtacaa cttatccact ccggaataat
      541 cgaaagacaa cttttgccag cttccttttc ggcttaaatc gtattcatcc gaaaatcctt
      601 gtcctgtatg ggctggactg gtaaagattt gtacgtgaac accatcaaaa tcctcgctga
      661 cataacacca ggccgaaaat cttcccttcc cctgactagg ctggtaaaaa gcggttaaaa
      721 actccatccg gtcgaaatgc ggctcgtaat agccccgaca agtggatgaa atcttatagc
      781 cccaacaatc ctgtagttct ccggggggat cgcccggcaa atccttgatc aattcatagt
      841 tatcggtggc gtaagttgcc ggatctttcg gatcgtagcc ttgttttcca tagagcggat
      901 agcaaaatac caccaaagcc attaatacca tggccgcatt aataatcgta aaagtcttac
      961 aaaaaccggc aggtaaagac aagtgttctt ccttgcggcc tcctgccttg gacaaaaaca
     1021 aaaagctcag acaaaagact atgaggccgc caatttgcaa aaaaaccact tccaccatca
     1081 tcagcatagc aaacataaaa gcgatggtgc ccgcgtacaa acgctcgttt ttatcgcgaa
     1141 gactgagtgg aaaagcaata agcaagatca ataaacagag tagtcccggt atgccggttt
     1201 ccaataatgc ctgcaagtag gtgttgtgca cgtgggaatt gccctgaata acttcggggc
     1261 tcaagcggtt ctgttcgtat ttttctttca gcagatcttt cacgtcacct atgcccgctc
     1321 caaataaaaa ccgattgttt tcactcaaaa cctgcatacc ggaataccat atgcccagac
     1381 ggggatcttt gctttggagg gaagagcttt cggtcagttt tcccggtcgt atggattcaa
     1441 aacggggatg atatacatat aaggctatca tggaagaaac gccgagcaat attaccgcca
     1501 gggcaaattt tcgatgctga tgcttgttca gcgcattata tacataaaac gaagcaacca
     1561 aaaccaatat gataagagca attcggcctc ccaaaaccag catcagcgag gtacaaacca
     1621 gcgcatagac aataaagcac tgccagtagc aatatttacc catttttgga atcagatctt
     1681 tgcgcagata taacaggctg agcagggcaa gcatctgatt aatgccaaaa taggtcctgt
     1741 gtttgaaaag gaggaaaaat tctaaaaaag ctttccaggg aaacttgtga atcatgttac
     1801 ggctcctgaa atcgctcaaa tattcaatga taggtatgcc caaagaaaag agtaaactaa
     1861 acacattacc ggccacaaat gccaccaaca aaggtttaag tttataatcc ttgctgaatc
     1921 cgatggaaag catcagagga acagcagcga tgaccactct cctttctatg actttgtagg
     1981 catattccag atttgtagaa taaaagacag aaataagcat caacagagca gaaacccata
     2041 agatgtaaac tgtccaatgt tgtttgtcga ggcgaaattc aaggtagcgt ttactgagaa
     2101 taacatctat gatccaacta accaaccagc agaaaaacac caaaatcagc caacgatcct
     2161 gcaaactgag gtaaaaagcg agcagtaagg cgaagtaata gttaaactta cccgtgaaag
     2221 ctgtgatttt tggcataccc ataactaaaa aatcaaggaa tactctcgta tatgtccctg
     2281 aagaacaaga ttataataaa gagctatccc caccaaaaca ataataagtt ccggtatcca
     2341 gcgaggtttt atataataga tcaatgtcgg aatcagaaag atttgcacga cgttcagcat
     2401 ttcccaaagc ctgaaggcaa ataccggcag gccaaaaaac aaataaaaaa ccccaacgga
     2461 aatcatgttg attttcagaa acaacacgct gtatttgttt ttctcgagca aatccgtacg
     2521 ataaaacaat agaaaaaccg aaagtatgag ttgaaatatt atcgtcaggt taattttgtt
     2581 gatggcgtgt ttgagcctta attgctctgt aatatagatg cttaatttct cggagtaaac
     2641 tcccaaatcg tatttcaaga gcaaagaaat cgggtcaaaa tggaagacac tcagcaaaaa
     2701 agggacaaca atgacagcta tatagactac cggatttatt tttttggtgt tcaaaaaata
     2761 gaagggcaaa aatataatgg ccgaataatg gaccaatatt gccagtagca tgatcaacaa
     2821 atatttccag ggttttcttt cgaccaaagg ttcgaaagcg agcattgcca aagctgcggc
     2881 caagcctgca cgaacctgta tgatttcgtg cagcatataa gtattacaga gatatataaa
     2941 cagggagaga aatatgaatt ccgaatattt gactatggcc ttgactttga gagttaccga
     3001 taaaaaagca aacaacacca tcaaaaaggt aaatccccaa ccgggcagat aggttttaat
     3061 aaacgaggcc aaaatcaaaa aagtgggttc cactttggcg gctttgaaaa aggcccccgg
     3121 attgctgaaa agtgtttgga aaggcagagc cttgtcgtaa aaagcctgaa aaatgctgtg
     3181 gtccttgctt acatcgttac cccggaatcc ggcgaatgca atcaaaaaca gagtcatcgc
     3241 caacagcagc cattgcctgt tttttttcat ctcctcccct gcgagacaga gtatcaggaa
     3301 aacagcaatt acgattatat agacggtcat tctgaaaaac gaacaattaa tacccggata
     3361 agccccatcg gattaaggcc gatcttgaaa acgcaaatgt actatttttt tgctcaataa
     3421 gcccaacgaa aaagaatgat cgatagcgta gttcaacatc ccaatgcaac ttttttctcc
     3481 taagaggcga aaaatacttc tttcggagaa agaagccggg gttttctccg ggtcttcaat
     3541 taattcgtca gcgaatgtcc gcaccgggca tcaatttact ttgttcctcg agcctataat
     3601 aaaatagagt atggaaccga agaaggggaa aagcagcaca ataatgaccc acaagagttt
     3661 atcgtttcca taaaactgac atctcaaaat atcaattaat gcgatgaggg gcaatataat
     3721 aaccggtaaa atacaaagta aaacgataag tacgataaga cctgcagcca tagtcatggt
     3781 aataaaacga aagcttgtta ataatcaaat gccgccggac catgcaaaaa gatgtccaga
     3841 ctcgatgcaa ataaaagata aatttgggga atcaggaaat taaaattctg tcatttattt
     3901 ttctcaaaat gtgtaaattt gaaaggtctt ccagtcgatc cctttaatgg aagactaaag
     3961 caccaaaata tcataaaaac cgattaatgg tgaaattgat atgaaaacaa accatcaaga
     4021 tataatcaaa agttattaga tacaggtgag agattttgct cgcaatgctt aaaattattt
     4081 aaaaaataag cgttatttct atataataca tgataccgtt tgatcatatt atttactttt
     4141 gtacttgaac cttgactata aatattgttt atgaaaagaa ttttattact aatcgtatta
     4201 ttgacggtct ttgtatgtct gtcctccacc cgtgcgcaga caatctattt acggagcggc
     4261 caaaagattc ctatcgaggg gaatggattg cattcaattg ttccattaga aaatgggtat
     4321 gcgatggcat ttaaagggaa ggatggtcaa attatgcatg tgtttcattt tgatgattta
     4381 tcttcaacaa agtggaccgg aacgtctgat gctgttgaaa cactttctag cgataagcaa
     4441 atgatcgtgt acttagctga gaagaaagaa ctaatcatca ggaatggagt agaagatgga
     4501 gatatctgtg ttttttcttt agacggttcg cttgtggctc gtggaaaagg cagatcctta
     4561 gacatctcta caatggcaga tggtctatac atagtgagtt acaacaaggt gtttaatgct
     4621 aaatttttga agaaatgaaa aaaaatgtat taacgacctt tgctgcatca tttctgatta
     4681 tcgcctcaaa cctggcacaa gacacgatta tcaagacaat agacgtattt agaaacaaca
     4741 cagctagtgt ttcctacttg ctggattcga tcaagcgtat agattttgta aaggttccat
     4801 acaccaaaac agatttttat gctggtacgc ttaatcttac agcgtctgcc ggtattcatt
     4861 caaaattcca tgttcaaagc aatattaaat gggatgtcgt aacagatgcc gaatgggtaa
     4921 aaggattcat ccgatatgat gagaacctat atgcaaagga aaatggttac tgttatattg
     4981 taacgacagc ccataccgga cagcaaaatc gcaaggcatc actatctgtg atcaattctg
     5041 acaatcaatg ttctaaaacg ataaccatca ttcaaaaacc gtatgaattg ttgttcttaa
     5101 atagtaatga tacagaatat tcattagata ttggttgcac cgacagttct tttaatttat
     5161 atttttctcc gaattttgat tggcagctga cctataaacc ttcctggata aaacataaca
     5221 ttgtagctcc gcaggtgcag ggcaatgaat caaccgatac tcttttttca cgagaaataa
     5281 gtgtcgatga tgcacaatct tttactgtgg ggcaaaatgt cccctgggaa gatcgacaag
     5341 gacgggttgt tattgaaggt catggacaag agatcgtcgc ccacataact cagaaaggtc
     5401 tgaattcacc gtatgaggat atgtatacca tattaggttc tccgaactct tgcagactgt
     5461 acacgattga tgatttcggt tttatcatga acgcaatcag taacgacctt gaagccgctg
     5521 acgcaattat gccggataat gtttataatt ggttcggagt ttgcggtgac ttaacttccc
     5581 gtaccggagg ttaccgaaac ccgtttattc gctatcagtc ctgttataac gtaatagatc
     5641 ttgctaatat aattctaaga caggatgacc ctaacctgga tcccgccttt agagccaaga
     5701 ttggcgaggc gtatgttata cgcgcatttg cctatatgaa tttagcccca tattatcagt
     5761 tcggctatga ggctttgggt gccaaagtag attcgtctct ctgtgtccct cttgtaaacc
     5821 ctggtataga tcctgccgat aatccacgtg caactgttac cgaggtatat cagcaaatca
     5881 tttctgacct aacaacagct atctataaac tagatggatg gcaacgtacc aataaaagta
     5941 aaatagatca gcaggttgca tacggtcttc gtgcgcgtgc ttaccttacc atgggagaat
     6001 gggccaaagc tgcagaagat gctgaaaaag ccatgagggg ttttcagtgt gcaaccagag
     6061 aagaagtctc taagcccttc ttatacgaca ttgatgaaca caactggatc tggggttacg
     6121 atatgacaga agaaatggcc aactccaatc cttatgctac aagtagctcc cttttgcgtt
     6181 cattctctgc caatgcatat tcaacaggac tccagattta ttgttatata aacaacattc
     6241 tttacgataa gatttcgaaa accgatgtac gtaagggatg gtgggtgaac gatagtcttt
     6301 actctccact gctagatagc ctgacgtggg atggactgaa aggccaggaa atagctacgg
     6361 acaccatctt aaaagtaaaa gaaccctacc ttccttatac caatgtgaag ttcggctgta
     6421 acacatgtgg tacgcatacc aacgacgaag actggtgttg gatgcgtgca gaggaaatgc
     6481 ttttaattca agtagaaggc cttgcaagga gtggctgtga tagtattgct agttctatcc
     6541 tttccgcatt tgtaaaaaat aatcgagacc ctgaatacaa tatttcaaac agcggtcgtt
     6601 cgcttttgga tgaaatctgg tttcagcgac gggttgagct ttggggcgaa ggtttttcaa
     6661 atgcagatac acgtcgctta aacaaacctc ttgtccgctt taccgacgct tctacaagca
     6721 atgttcctga gaatttccga tttaacatgg aagctcgcga tagttggtgg ctgatgcgtt
     6781 tccccgagaa agtattgaat aataacccgg ggattgtaga caacacagaa gggaatttgc
     6841 caatacttaa tcaaaacccc gaacttagag atggtgttac cgattaacat aaaaagaaat
     6901 acaatgaata aatctttaag aaaaattctt ttaacggcta tatttctctc tatagcatct
     6961 tcgttgttaa agtctcagaa ttatatctgt atagttcagg aaggaaaaac agactccatc
     7021 aatgcagaaa tcatcgacag cattgttatc actgaatgga aatcattagg aataggacag
     7081 ttttacgatc aattcgtcct aaaccatgtt cagccagttg aaatactcaa gtcggaagaa
     7141 gaggataaat accgagtact taacccctat caggacctga gtgcagaaga atggggcgaa
     7201 tggatgggtg ggacagattg tccttacatt gagttctggt cttatgactc caataattct
     7261 acctatttaa agtgggacaa attctggtac agtaccttgt tgtataataa cactggtatc
     7321 gatctcaagg cttattatcc ttcttttctt tcttcttctt ttgcatacct tgaccttaag
     7381 agcacctggt tcatggaaaa tgttgctgtc ttatatcctt attggtttgt tgacggttta
     7441 ggaggttatg gtgccggtga tgcggtatat atttcattac ccggtggcac tgatctggtt
     7501 gaattatttt ttgattcttc aacacgttca gctccggcga aacaagctgt acaggttaaa
     7561 gacaccctcc cacccagtca ttcaacgcat acaaaaaatg agattatgaa atacaatgcg
     7621 acccatcaat aagaatggct actaatctat gatattatgt taatctataa gatgtgaatg
     7681 ctttttggcg ttacgtggat gtcgggcaag tcataaacca aaaaaagcca acacctgtaa
     7741 cacagcctgt tagctttttt cctgtgacct cggaggggct cgaaccctcg acccattgat
     7801 taagagtcaa ttgctctacc aactgagcta cgaagtcttg ttctgttttt tgaacgggcg
     7861 caaaagtacg ttatttatgc taaacggcaa aagctttaaa ggctttttct tatttttttt
     7921 ccaattcgcc cagttccatc cagcgcaggg cttttttgtc gagttcttga ttcagtctgg
     7981 aaagctcaat gcatttttgt tcgatcgcgc caccttctaa ttgccccgaa gccagatcgt
     8041 tttctattag agcaatgttt tcttcgattt gtgcaatttc cttttcgaga gcgataaatt
     8101 cctgttcttc gcggtaagtt cgttttcggt tggaggaact ttcctctttt ttcggttcaa
     8161 tatccgggag gttagttttg ctttttcgct ccgattcttt ttcttttcta agtaaagctt
     8221 cctgctgttc tttccactct cggtattggg tgtaattgcc cggaaaatcc cggacgatgg
     8281 catcgccttc gaaaaccagc aaatggtcca ctattttgtc cataaaaaaa cggtcgtgcg
     8341 aaaccaccaa tacacaacct ttgaacgaaa cgaggtattc ttcgaggatg ttcaggctca
     8401 aaatatccag atcgttggtg ggttcgtcca acaccaaaaa attgggatta gtcatcaata
     8461 cggtacacaa atacagccgg cgcttttcgc cgccacttaa cttggaggca taattgtgct
     8521 ggacttcggg actgaataaa aaatgagtga gaaattgaga agcactcagt ttcttaccgt
     8581 ttcccaggga aatttcttcg gcgatatctc ggaccacatc gatcactttt ttattttcgt
     8641 caaacgacag gccttcctgg ctaaaatagc cgaaacgtac ggtactgccg atatctatac
     8701 tgccccggtc gcaaggctcc tctcccatca gcaggcggag aaaggtggtt ttaccgctgc
     8761 cgttggcgcc tactacgccg actttttcga aaggagcaaa ctgataataa aagtcttcta
     8821 aaagtttcag ctcaccgtag gattttgaga tgtatttggc ttcgagcact ttggagccca
     8881 gataactggc ttttactccc aaacgcaact gttctgtttt gtgccgttgc gatacccgtt
     8941 cttccagttc ataaaaagcg tcgctgcgag ctttggcttt ggttcctctg gcctgaggca
     9001 tacgtctcat ccagtcgagt tctttgcgat acagattttg cgccctttcc cattcggcat
     9061 tggaaatctc gagtctttcg cggcgctttt ccagataata ggcgtaattg ccgttgtagc
     9121 aataaagttg tttgtcttcg agctccagta tcgtattgca gacccggtcc agaaaatacc
     9181 ggtcgtgagt caccatcaaa agagttatcc ccgatttggt caggtaattt tccagccact
     9241 cgatcatatc caggtccagg tggttggtgg gttcgtcgag gataagcaaa tcgggttcgg
     9301 taatcagcac gttggccaaa gccagacgct taagctctcc tcccgacagt tgtccgactt
     9361 tttgggtaaa attcggaatc ttcagtttgc ttaggatttg ttttattctg acttcgtaat
     9421 cccaggcttt gtagtgatcc atcagatgca acagttcttc ggcttcggca tccatggatt
     9481 tgccggctat ttcctgcatt ttgagctcgt aacgggctat ggtttcggtc acctcgttgg
     9541 cggcttgaaa acaagcttga aaaacggtca aaccgaccgg aaattcgggt gtttgtttta
     9601 aataggctat tttcagacca cgtcgaaaaa ccacggaacc ctcctcgtaa tctttgttcc
     9661 cggccaaaat atcgagcaag gtggatttgc ccttcccgtt ttgagcaatc agtgcaaccc
     9721 gttgttgctg tgcaatgcca aaggaaattt tgtgaaaaag gaccaaatcg ccaaaggatt
     9781 tgctcagatt ctcgatttga acaaaaggct gtaatttact cataataaat ccttaagaca
     9841 ggctgccgta tagctttgcc tgctcaaagc caactcttcc ggcgtaccgc tgaagagtat
     9901 tctgccgcct tctttgcctc cttcgggccc catatcgatg atgtggtcgg ccattttgat
     9961 gacgtccggg ttgtgttcaa tgataatgac cgtattgcct ttatccacca aacggttgac
    10021 cacctgcatc aataccctga tgtcgtcgaa atgtaagccg gtagtgggct cgtctaaaat
    10081 gtacagggtg ttgcccgtat cccgtttgga aagctctgcg gccagcttga cacgttgaga
    10141 ttctccgccc gacaaagtgt tggaaggttg tcccagttta atatagccca ggcccacctc
    10201 ttgaatggtt ttaattttat gcaagatctg cggctgattc tcaaagaaat cgactgccat
    10261 attgatggtc atgtccagca catcggcaat ggatttaccc ttaaagcgaa tctccagagt
    10321 ttcacggttg tagcgtttgc cccggcaaga ctcacagggc acaaaaactt cgggcaaaaa
    10381 attcatttct atagccctgt aaccgttccc cttacaagtt tcgcaacgtc ctccggctac
    10441 gttaaaagaa aagcgccccg gtttgtaggc ccggattttt gattccggca gatttacaaa
    10501 tagattacga atgtcggaaa acactccgca ataggtagaa ggattggagc gcggattgcg
    10561 tcccagggga gactgatcca catccaccac tttatcgatg aattccaggc cttcgatgct
    10621 atcgtaaggc aggggttcct gatgactgcg ataaaaatgt ttgctcaggg cagcacgcag
    10681 ggtgtgattg atcaggctgg attttccact gccggaaacc cctgtaacta caattaattt
    10741 tcccagggga aaatcaacgt tcacttgctt gaggttgtta cctcgacagt tcttcaggct
    10801 caagacacgg ccatttcccg ggcgatacgc tgtaggagcc tttatccgta gtttcccgtt
    10861 caggtaatcg gaagtcaagg tagaagcctg cagcaagtcc ttgggactgc ccgcaaaact
    10921 cagatgtcct cccagtctgc ctgctctcgg ccccaggtcc agtatgtaat cggaagccag
    10981 catcatttcc ttatcgtgct ctaccacaat aatcgaatta cctttgtccc tgagggtttt
    11041 cagggagtct atcagacgtc ggttgtctct gtggtgcagg cctatgctgg gttcatccag
    11101 aatatacagc acattgacca gttgggagcc gatttgcgtg gccaggcgga tacgttggct
    11161 ttcgcctccc gatagccctg ccgaaggcct gttaagcgaa agataatcca ggccaacgtc
    11221 caacaaaaat tgcagacgtt gcgaaatttc ctttagtatt tcggaggcta tgcgtttttg
    11281 tttatcgttc aaatgacgat ccagatcttt tatccacaga gccagttctt tgatgtccat
    11341 cgcggccagt tctgcaatgt ttttaccggc caggcgataa tgcagggctt cttttttgag
    11401 ccgcaaaccc tgacattcgg gacatacggc agtactgatg aattgttcgg cccatttttg
    11461 cgcatccgac ggactgtcgt cgtcttgctg cagttggatg tatttgacga caccttcgta
    11521 gtgtacattg taattataca gacccagcgt agtgtttttg ataggcagcc gttcttcgca
    11581 gccgttcaaa atctcatcca tggcgtcggg agcgatatcc ctgatgggtg tttttagttt
    11641 aaaaccgtgt ttttcgccca gagcctctat ttgccagaac aacatactgt ttctgtattt
    11701 gcccagaggc actattcctc cttcaaaaat ggatttgctg tcgtcgggaa taattttatt
    11761 gaaatcgatc tggttgatta tacccaaccc cttgcatcga ggacaagctc cctggggaga
    11821 attgaacgaa aaagaatgag gggcgggatc gccgtaggaa aggcctgtaa ccggacacat
    11881 cagttgacta ctgtaatgtc tgagttcgtc ttgatccttg tcgtatatca ttatcaggcc
    11941 tttgccttgt ttgaatgcta cctgcaagct ttttttaagc cggtctctgt cgttaggact
    12001 gacgatcaat ttatcgacaa gcacttccac atcgtggttc ttgtagcggt ccagcctcat
    12061 gcccggaatg atttcctgta attctccgtc aacacgaaca tttagatagc cttttttcct
    12121 gagcgtttcg aacaattcct tgtaatggcc tttgcgggaa cgcacaacag gggccaacac
    12181 ataaatggct ttgttgttgt acttttccaa aatcagctcc aacacttgtt cctcggtata
    12241 cttaaccatc ttctcaccgc tcaggtaaga ataagcctct cccgccctgg cgtaaagcag
    12301 acggagataa tcgtatactt cggtctgggt accgacggta gagcggggat tcttgttcgt
    12361 ggttttttgt tctatggaaa taaccgggct caaccccgtt atcttatcca catcggggcg
    12421 ttcgcgattg cccagaaagt tgcgggcgta ggccgagaaa gtctcaatat aacgacgttg
    12481 cccttcggca aaaagggtat caaaagccaa agaggattta ccgctgccac ttaagccggt
    12541 aatcaccgtg agactattgc ggggaatctg aacgtcgatg tttttcagat tatgttcccg
    12601 cgccccgtct acaaaattat tgtctattta tgcgtcgtcc attgtggttt acccatcgac
    12661 tacttgtttc tgatccagtt tttttgatgg atgtagagat tcttgacatc aaaatactgg
    12721 gaagactcca tgggaatggc gatgttgtaa attttacccc gcggattgtt cagccggcta
    12781 ccgcgcagcc aggggttggc ctccttaaat tgcaggtagg ttaagccgtg ttctttggca
    12841 aattccaccc agtcaccgac agatttatga atactgacgt agttgtattg caaaggcata
    12901 tacaaatatt cagctgaaaa gataaaaccg tatttggcgg ggttttccat gaaatccttg
    12961 gcggccatta tacggaacac gtaacgcgaa gtctcctcgt tcaaatataa atccagcgcg
    13021 ttgtccacac cctgcttttc cagttcgttg gctatgcgat ttatgccgcc gttataggct
    13081 gctgccgccg tactccagtt gccgaattta cggtaggcat ccttaaggta tttacaggcc
    13141 gctacggtag atttttccaa gtggtaacgt tcgtccacct gatcgtcaac ctgcaggccg
    13201 tattcgcgag ccgtttcggg cataaattgc catattccgg ccgctcccgc gggagacaaa
    13261 gctccggaat tcagattgct ttcgatgcaa gccaggtatt taaaatcgtc ggggacttgg
    13321 ttggccttaa gtatgggctc tatgagggga aaaatccggt tggctttttt tatgagctgg
    13381 atggaagacg agtgcatgta attcatcgcc aaaagctctc tgtcgaagcg ttccctcaaa
    13441 tcccaacgcc tcaggtctat gcgttcacca cagaattctg tccggagagg aagatctatc
    13501 ggatgtatca aagcctgagg ataaatgctc tgagccatcg aaaagaaacc gttgtacatt
    13561 aaaaaaacaa cagcaattat atacttgttc aatttcatca ttctaataaa tttatgtctc
    13621 cttttttccg gtattctata ccatcggagc cggtagcgct gatgatgtaa aaatacacgc
    13681 cgggagatac cggtttacct ttaaaagtac catcccaacc aagggaagga tctgtccatt
    13741 cgaacaacac attgccccaa cgattcagga tgcgtccatg aaatttaatc agcgatttgt
    13801 aggccacttt aaattcgtcg ttgtctcctg gagtggaacg agggctgaaa aaattagggc
    13861 aatccagcga agattccgtt acccgcacgg tgaaggtggc tgaatcttta caaatattgt
    13921 tactggccaa taacttcact tcaaagctgc cggcctggtt aaaagtgtaa ttgatatcgg
    13981 tctctgtgcg gcgcaggtat tcctcctttg agccgggatt ggtgtaaata taccattcgg
    14041 tataacgggt tgccggattc acattactgt aaaaatccag tttaaaggga gccgatcccg
    14101 taagcgaacc ggctgaacct gctcctattt cgttgtcggc ctgctccctt tcctcaggaa
    14161 ccgctttggc atgagtactt acgcgaacag cctgatagct tgtttcgatg ctttttgcac
    14221 tcaagccgaa atgggcggca aattggtcgc cttcgagttt gaatctggta ttctgcagag
    14281 gagcatccac gatgaaaccg gacaaaaagc cttctttatt gctgttttta tcgacaggca
    14341 ggtattgctc cttctcttcg tcccattcca agtcagtcca agtcaggctg tgaattctgt
    14401 ctacggtacg tcgcgccggg cttccgctca gggcataata gctcaaatcc tcctgaatac
    14461 cttcgaccac gagctgcagg ttgctgcaaa catcctcggc attctcaaca ccttcaagct
    14521 tagtataatc cagcttatga gctgaatagt cgatgatata gacggccttt atttgatctc
    14581 cctgcgacac ataatatccg taagcaccgg gattctttaa cacagtggcg tcactttcaa
    14641 gactgatatc ggtaaggggg actgcctggg catcctcaag gccggtttgg taacgatacc
    14701 aggtccaatt tgacggatct gccgtgttga agcgcaattc tgcttgttga agtccatcga
    14761 agatatagac cttctccagg cctgtgcttg ctgtaggaat gtattcgtaa ggccggccgt
    14821 tcgatccccc gctggccgtg aaagcagcat tggcaggcag actaagtata aagaataaaa
    14881 agaataaatt aaaaaccaga tacttaagtg atctctttcg atgcatatca gagtttctaa
    14941 aaaaagcgca caaagataga tataaataat caaaaaataa aattaccctg ttttcatgta
    15001 ggcctcgcag attcagcatc cctacgcaac tagttgaatt tactgtctgt gcggtattat
    15061 tatactctga tttgcaaaac aacagatttt ggctacattt gcgcactgga ttagtgaccc
    15121 tgccctgtgg agggccggca ctataagcaa ccaaaaacaa cgctgatgaa gttacgtccc
    15181 ataattgcct tgtgtacctg cctggttttt tttactctgg ctgtaaaggc tcaggaaaat
    15241 aatattccgg tagttaaact gtttgacaaa gagtactata aatacgaggt gcaagcttct
    15301 gaaaccttgt atggtctgag ccggcgcttt aatgtaagcc aggaagaaat catagccatg
    15361 aatccttttt tgatcgaagg gctcaaaacc gggcaaatcc tactcattcc tgtgaaaatt
    15421 ttcgaaccgg acaaggatga acttccggaa aaaaccccgg ataattcggc tgaaaaatcc
    15481 caggagaaaa caccgggtaa atcttgcgga aaaagctctc aaaaatccct caaacctaac
    15541 gaagattctc tatcagcagc atcagattct tccgtgctgt ttgaaaaatg ggaatatgag
    15601 tattatactg tagaaaaatg gagagagaat ttgagtgata ttgccgacaa tttcagcgtt
    15661 gatatcgacg aaatcagact tcataatccg aatgtacccg acaggctgcc aagaggctcc
    15721 ttgcttatga taccgatcaa aaaaattgag gttgtttccg aaatcaagac agcagaagag
    15781 gaaccttccg aggacctcga accaaagatc tttcgtaaac ccaacatagc cctgctttta
    15841 cccttcatgc tcgacgatac aaccgcaaac tccatagaac gctatgtgga attttacgaa
    15901 ggtgtactca tggctgcaga cagcctgaaa caactcaatt tttcctttga gctgtcggtt
    15961 ttcgacacag gcaatacact ggaatcgctc aggaaggtgt tgacttccgg ttggctggac
    16021 agggccaatt acgttattgc tgcagtcaat aacgaacaaa tgatgtttct gtccgattgg
    16081 tctgtccgtg aaaagaaata cctaatacta cctttcagtt cccgaattcc tgaaactgag
    16141 ttcaatccct atatttatca gataaatcct ccccattcgt tgaatcatca ggctctgttg
    16201 gagctggaca cctcctacta tgccggcaaa aacattctgt ttattcgcac cgggaatgaa
    16261 tcgtccgatg aacgtcgcga attgttcgac actttccaaa tcagtttaaa acactacgga
    16321 attccataca tagaaataac agataacgaa ccgcctgcgc acatagctta cagttttccc
    16381 gacaccattg cccgccattt aagcttagac aaagaaaacc tgatcgttcc tgctcctttt
    16441 cccatgaccg aaagcaaccg ggtaattacg atgataggct ccgcagccaa tataaaatcc
    16501 aaggcccgta ttactttgtt ggg
//
LOCUS       DAOSGL010000012        16082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_2974_length_16082_cov_4.410370, whole genome shotgun sequence.
ACCESSION   DAOSGL010000012 DAOSGL010000000
VERSION     DAOSGL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16082
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2974_length_16082_cov_4.410370"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKY68_00845"
     CDS             complement(<1..271)
                     /locus_tag="PKY68_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOH23620.1"
                     /translation="MLKIVFASHNKYKKQEVADILGNSVELIDLGDLNCSEDIPETQD
                     SLQGNALQKARFVYEKYGLNCFADDTGLEVEALNGAPGVHSARYAG"
     gene            complement(280..1221)
                     /gene="nadA"
                     /locus_tag="PKY68_00850"
     CDS             complement(280..1221)
                     /gene="nadA"
                     /locus_tag="PKY68_00850"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOH23621.1"
                     /translation="MDREVLKSEIKRLRSERNAVIMAHYYQIREIQEIADFVGDSLDL
                     AQQAAKTDADVIVLCGVHFMAETAKIISPNKKVLIPDAGAGCSLAESCPAGEFEAFIK
                     AHPDHTVISYVNTSAAVKALSDLVVTSSNALQIINSLPQDEKIIFGPDRNLGNYIKSL
                     SGRDMLIWDGACHVHEQFSLQKILELKKQHPQAEIIAHPECKQDLLKVSDFIGSTSAQ
                     VAYVQKAEAGEFIVATECGVLFEMSRKCPGKLFIPAPPDDDSKACNECTFMRMHNLEK
                     LYRCLRDEQPEVLVEEQLRRQALRSIQRMLNISASLK"
     gene            1294..3129
                     /locus_tag="PKY68_00855"
     CDS             1294..3129
                     /locus_tag="PKY68_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOH23622.1"
                     /translation="MIVTVKELDRAVVRFSGDSGDGMQLTGTLFSTLSAIFGNEISTF
                     PDYPAEIRAPQGTLSGVSGFQVHIGNKVYTPGDKADVLVAMNPAALKVNVKNIKKDAV
                     ILVDADAFDESGLKKALYKTEDPIEELKLGAAQLVAVPISTLVKESLADTDMGNKEIL
                     RSKNMFALGLICWLFNKPIEEAMRLLHRKFGKKPGVLQANIKVLTDGFNYGHNIHASV
                     STYNIRPTDMPKGIYTDVKGNEATAWGLIAAAQQAGLQLFLGSYPITPASEILQELAA
                     RKDLGVITVQAEDEIAGICTAIGASFAGNLAATSTSGPGLALKSEGINLAVMAELPLV
                     IIDVQRGGPSTGLPTKTEQSDLLQALYGRNGESPLVVLAASTPSNCFDFAYMASKLAL
                     EHMTPVILLTDGFLANGSSAWRLPDLSQFPAINPPFVHKEMQGKWAPYLRNEQGSRYW
                     AIPGTEGFAHRIGGLEKDSRTGAISTDPANHQLMTDLRQQKIDNIAKVIPPLQLSGNS
                     NADLLVVGWGGTYGHLYSAVRQFNEQGRQAALAHFNYINPLPANTEEVLNKFKKIVVC
                     ELNSGQFASHLRSKLKLKGEILQYNKVQGQPFTVEELLRHFSTIL"
     gene            3140..4150
                     /locus_tag="PKY68_00860"
     CDS             3140..4150
                     /locus_tag="PKY68_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOH23623.1"
                     /translation="MQTSYTVNDFKSDQYVRWCPGCGDHALLNSIQRAMAEIGVSPDN
                     TAVISGIGCSSRLPYYVNTYGFHTIHGRASAIATGVKVANPRLAVWQITGDGDCLAIG
                     GNHFIHAVRRNVDINIVLVNNGIYGLTKGQYAPTSKRGFISKSSPYGTIEDPFNASNL
                     CLGAGGQFFARILDTDLKNSQEVLKLAAQHKGCSVVEALVNCVIFNDGTHKEVSAKEV
                     REDYTIFLRHGEPMIFGKDRDKGLIVDGFKLKVVKIGENGITENDILVHDAKCPDKTL
                     HFKLGHMRGPELPLALGVIRDTEAPVYDEEVYTQFEEIKPKRNYKSLNEFFMSLETWE
                     IK"
     gene            4147..5352
                     /locus_tag="PKY68_00865"
     CDS             4147..5352
                     /locus_tag="PKY68_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOH23624.1"
                     /translation="MKLRQLCLIGLCFWCFNLKSEQPETYTPYGLQNNMPLFYRQAKE
                     AITFSMAWEHQGNAKQLQQKKRFTKWKKQARQVLFDCMGPLPPPARDFDALVLAGEQR
                     EGYEGGRPYLAQKILLNITSFERIPAYLLIPEGEGPFPAVVMLHDHGAKFSIGKEKMV
                     KPINPSEELEKECNQWSVQCYDSIYTGDYFASQGYVVLAIDALFWSERGSVEGHDYNR
                     QQALASNMFQLGRTWCGHITNDDIQSVEYLCSLPYVDIERIAALGFSMGAHRAWMLAA
                     ASDKVSAAVAVCWMNTTEYLMTLDNNQNKGGSAYSMLLPEIRNYLDYPHVASIACPKP
                     MYFINGSRDKLFPVQGCLDAFETMRRVWESRGAGDRLKTEIWDSPHFFSRAMHEQSLL
                     FLNKFLRKH"
     gene            5659..7269
                     /gene="pckA"
                     /locus_tag="PKY68_00870"
     CDS             5659..7269
                     /gene="pckA"
                     /locus_tag="PKY68_00870"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOH23625.1"
                     /translation="MKSLDLTKYGIKGTTEIVHNPSYEQLFKDETDPNLQGFERGQVT
                     EMGAVNVMTGVYTGRSPKDKFIVMDDVSRDTIWWTSDEYKNDNKPLPKASWGVLKDIV
                     TKQLSNKKLYVVDTFCGANEASRLKVRFIMEVAWQAHFVKNMFIRPTEEELKNYGEPD
                     FVSLNGSKTCAGAQYKELGLNSETFTVFNLTEKMQVIGGTWYGGEMKKGIFTLMNYYL
                     PLQGIASMHCSANVGKDNDTAIFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDEGIFNF
                     EGGCYAKTINLSKEQEPDIWNAIRRDALLENVTVDANGKIDFNDSSVTENTRVSYPIY
                     HIDNIVKPVSKAGPAKKVIFLSADAFGVLPPVSILTPEQAQYYFLSGFTAKLAGTERG
                     ITEPTPTFSACFGAAFLALHPTKYAQELVKRMQASGATAYLVNTGWNGTGKRITIKDT
                     RGIIDAILDGSIDKAATKDIAYFNLKVPTALPNVNPAILDPRDTYADGKVWDEKAKDL
                     ASRFIKNFDKFTKNNEAGQKLVAAGPKL"
     gene            complement(7474..8748)
                     /gene="serS"
                     /locus_tag="PKY68_00875"
     CDS             complement(7474..8748)
                     /gene="serS"
                     /locus_tag="PKY68_00875"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOH23626.1"
                     /translation="MLTLKYLSENTRECIERLAVKHFDAEALIGEILELDLQRRNAQT
                     RSDACAAELNTLSRSIGVLMQEGKKQEAAVVREKTLQLKEEGKSLETARKGAENRIQE
                     LLVQIPNLPHSSVPAGKGAEDNVTERSGGRMPDLPVDAMPHWDLAKKYDLIDFELGAK
                     ITGAGFPVYKGKGARLQRALVNYFLDRARQAGYLEIQPPYVVNAASGYGTGQLPDKEG
                     QMYHVGLDDLYLIPTAEVPVTNIYRDVILDEKDLPVKNTAYSACFRREAGAYGKDVRG
                     LNRLHQFDKVEIVCIEHPDRSYERLEKMVTHVQSLVEALELPWRILRLCGGDMGFTSA
                     LTFDFEVFSAAQQRWLEVSSVSNFESFQANRLKCRYRTAEGGKIQLCHTLNGSALALP
                     RVVAALLENNQTAEGIRIPEVLRPYTGFDIID"
     gene            complement(8812..8887)
                     /locus_tag="PKY68_00880"
     tRNA            complement(8812..8887)
                     /locus_tag="PKY68_00880"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8852..8854),aa:Pro,seq:cgg)
     gene            9025..9097
                     /locus_tag="PKY68_00885"
     tRNA            9025..9097
                     /locus_tag="PKY68_00885"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9058..9060,aa:Phe,seq:gaa)
     gene            9403..11406
                     /locus_tag="PKY68_00890"
     CDS             9403..11406
                     /locus_tag="PKY68_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase"
                     /protein_id="HOH23627.1"
                     /translation="MPSGKSSPTLELAKQVLIEGWQDRENNSIKLKISRDKRLSEQGY
                     SLSPGCITAKTHQGLLYGAYEYLRRRQIGLSLELPFSNPSYQRRLLNHWDNLDGSVER
                     GYAGPSIFWKGKQSMEPTAEDREHWRTYAAINASIGINGAVLNNVNAPPEALSLPVLK
                     RAAAIADELRPYGMVSYLSINFSTPMRLGGLKTADPLDSEVVDWWRAKIHEIYSLIPD
                     FGGFLVKASSEGMPGPGDFGRSHAQGANMLAELLKPYHGILMWRAFVYKPSDNDRAKQ
                     AYEEFMPLDGQFCDNVVVQIKNGPIDFQPREPFSPLFGALRKTAVLPELQITQEYLGQ
                     EHQLAYLGGLWEEFLQSDTYQSGPGSTVARCTDGSLFDQSLTAIAGVSNIGSDSNWCG
                     HPFAGANWYAFGRLAWNNRASAAGIAEEWLRLTFEPTQSPEGNSLSDEGPALDNDPAS
                     DQKNGNWEKEFLQPVLSMMLRSREAMVNYMMPLGLHHLFALDHHYGPGPWYDSPRQRK
                     DWTPPYYHQADAKGIGFDRSSGGSNAVSQYREPLRSQFDNPGSCPENLLLWFHHLPWE
                     HRLRNGNSLWEELCRRYDAGLQEVRRFQLIWDNMENRVDSGIFIQVQAKLRRQARDAQ
                     VWKDACLLYFQSINQLAFPEDMERPVHDLEVLKKTDMRYFMNR"
     gene            11490..12614
                     /locus_tag="PKY68_00895"
     CDS             11490..12614
                     /locus_tag="PKY68_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOH23628.1"
                     /translation="MQRTFLLLTTVVLSFCSHLTAQTTLRQATQNLFLIGVAVNNPQT
                     RGARPAEAEQISRHFSAIVPENCMKSAEIHPEEHRYNFEATDQMLAFGENNRQVVTGH
                     CLIWHSQLARWFCVDEQGEPVSAEVLKSRMREHIFSIMTRYKGRIKGYDVVNEAFEDN
                     GTYRNSPFYRILGKDFIRLAFEYAHQADPEAELYYNDYNMANPVKCDAVVKMVEELKA
                     AGCRIDAVGMQGHVHMDEPSVEAFETSILKLAATGVKVLITEWDISILPNPYRHTGAN
                     IADRFAYSTQNDPYREGIPDEVMKAWEQRVTELFALFLKHHRHIDRVTLWGLNDGNSW
                     RNNFPVRGRKDYPLLFDRDNQPKPVVQQMIELALKAKTKQ"
     gene            12805..15852
                     /locus_tag="PKY68_00900"
     CDS             12805..15852
                     /locus_tag="PKY68_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOH23629.1"
                     /translation="MRIPKLILGFCALFTTALTAQNGDQILDGIGETGLIARYTFKSN
                     ADDWSRNNLHGNIKGAGYRFATDSLFSTVLSLPEEAESYVIIPGESVSGEESLSISAW
                     IYPRSAGEKQVFFDFGKNGQSRLLVTLTGSGEKTTILAQVQTPAKTYELSSEALAINS
                     WHHLALVINLPSKSLNMFINGKIAKEVQKVELSLADLFDMETGKENKLLIGKSQSPDN
                     KPLNAALHDFRLYRIPLNEWQIARIYGIALNKEEPIVRRRRTEANRELPVFSPTTPQL
                     YNAFLTAVPDIEVETVTGQLPRLPRYLKGVYEKGIEGPDVRVIWPSPTDNSQVLTPGT
                     YEVTGKVSGTDLQPKALVTVKTAEKQNGPQRKVEAFQLDRVTLNTDCHGRNTKFIENR
                     DKFFNGLGATNPDNFLYMFRNAFGQQQPEGAEPLGVWDSQQTKLRGHATGHYLTALAQ
                     AYAGSAYNKDLQANFAQKMDYMVDVLYELAQLSGTPQSPGGTCVTDPCQVPPAEGKAS
                     YDSDLSEGGIRTDYWNWGKGFISAYPPDQFILLEQGATYGGSNSQVWAPYYTLHKILV
                     GLMDIYEICGNKKALDVVTGMGDWVHARLSNLPKETLINMWSRYIAGEFGGMNEAMAR
                     LSRLTGKKSYLETARLFDNINMFYGNAEHSHGLAKNVNSFRGFHANQHIPQIVGALEI
                     YRDSQAPEYFCIADNFWTMVTGDFMYSIGGVAGARNPNNAECFTAQPASLYENGFAAG
                     GQNETCATYNMLKLTSGLFMFDQRAELMDYYERGLYNHILASVAEHSPANTYHVPLRP
                     GSVKQFGNPHMNGFTCCNGTAIESSTKLQNSIYFKSTDNKTLYINLYVASTLQWKERK
                     ITITQSTAYPNEDQTKLTIQGKGKFELKVRVPRWAKKGFFVKINGKEIEVDAQPGTYL
                     SLNRKWKKGDVVELRMPFSFYLEPVMDQQNIASLFYGPVLLAAQEEEARTDWRKITLD
                     AKDIGKSITGDPSSLEFKIGDVLFKPFYNTYGRHSVYLDVSLK"
BASE COUNT         4329 a         4178 c         3683 g         3892 t
ORIGIN      
        1 ggccggcata acgggccgaa tggactcccg gtgctccgtt cagggcttcc acttcgagtc
       61 cggtatcgtc ggcaaagcaa ttcagaccgt acttttcata tacaaaacgg gccttttgca
      121 aagcgttgcc ctgcagacta tcctgagttt cgggaatatc ctcgctgcaa ttcaaatccc
      181 cgagatcgat caactcaacg gaattcccca aaatatcggc cacttcctgc ttcttgtact
      241 tattatggct ggcaaatacg attttcaaca tacggaaatt tatttgaggc ttgccgaaat
      301 gttgagcatt cgttggatag acctgagggc ttgccggcgc aattgctctt ccaccaaaac
      361 ctccggttgt tcatcccgga ggcagcgata aagcttttcc aggttatgca ttcgcataaa
      421 agtacattcg ttacaggctt tggagtcatc gtcaggagga gccggtatga ataacttccc
      481 gggacatttc cgcgacattt caaacaacac accacactcg gtggctacaa taaattcccc
      541 tgcttcggct ttttgtacat aggctacctg agctgaggta gaaccgataa aatcggagac
      601 cttcaacaag tcttgtttgc attcggggtg tgcaataatt tcagcctgag gatgttgctt
      661 tttgagttcc agaatttttt gcagtgaaaa ttgttcatgt acatgacagg ctccgtccca
      721 gataagcata tcgcgtccgc tcaaactttt aatataattc cccaggttac ggtcgggtcc
      781 aaaaataatc ttttcgtctt ggggcaggct atttatgatt tgcagggcat tgctcgaagt
      841 taccaccagg tcgctcagag cctttacagc cgccgaagta ttgacatagc tgataaccgt
      901 atgatcggga tgagccttga taaaagcttc aaattcgccg gccggacaac tctcggccag
      961 ggaacaaccc gctccggcat cgggaatcag gacttttttg tttggtgaaa ttattttagc
     1021 tgtctcagcc ataaaatgta ccccgcaaag cacaatgaca tcggcgtctg ttttggcagc
     1081 ctgctgtgcc agatccaagc tgtctcccac aaaatcagct atttcctgaa tctctcttat
     1141 ctgatagtaa tgcgccatga ttaccgcatt cctttcagat ctaagccgct ttatttctga
     1201 ttttaacact tctctatcca taatcagcct ttttacagcc gcaaaaatat aaaattaaac
     1261 cgtacctttg ctgcgttttt ttcagtatta gttatgattg taacggttaa agaattagac
     1321 agggccgttg tacgcttctc gggcgactca ggagacggca tgcaacttac cggaaccctg
     1381 ttctctaccc tttctgctat ttttggaaac gaaatttcca cttttcccga ttatccggcc
     1441 gaaatacggg ctccccaagg cactttgagc ggggtgtcgg gtttccaggt acacatcgga
     1501 aacaaagtgt atactcccgg cgacaaagcc gatgtactgg ttgccatgaa cccggcagct
     1561 ctcaaagtaa acgtaaaaaa catcaaaaaa gacgctgtta tcttggttga tgccgatgct
     1621 ttcgacgaaa gcggcttaaa aaaagccctt tacaaaacgg aagaccctat agaagagctc
     1681 aaattgggag cagcgcaact tgtggctgtc cccatttcaa ctctggtcaa agaaagtctg
     1741 gccgataccg atatgggtaa taaagaaatt ttacgcagta aaaacatgtt tgccctgggg
     1801 cttatttgct ggctcttcaa taaacccatc gaagaagcca tgcgtttgct ccatcgaaaa
     1861 ttcggcaaaa aaccgggtgt tttgcaagcc aacatcaaag tgctcaccga cggattcaac
     1921 tacgggcaca acattcatgc atccgtgtcg acctacaata ttcgtcccac cgacatgccc
     1981 aagggcattt ataccgacgt caaaggcaac gaggcaacgg cttgggggct gattgccgct
     2041 gcccaacagg ccggactgca actgtttttg ggcagctatc ctattactcc ggcttcggaa
     2101 atccttcagg aactagctgc ccgcaaagat ttgggtgtca ttaccgtaca ggccgaagac
     2161 gaaattgccg gaatctgtac ggccattgga gcttcttttg ccggtaacct ggcggctact
     2221 tccacttccg gccccggact ggccttaaaa tccgaaggca tcaacctggc tgtcatggct
     2281 gaattgcctc tggtcattat cgatgtgcaa cgcggagggc cttccaccgg attaccgacc
     2341 aaaaccgagc aatccgacct gctgcaagct ctttacggcc gcaacggaga atcacctctg
     2401 gttgttttgg ccgcaagtac cccttccaac tgcttcgatt tcgcttacat ggcctccaaa
     2461 ctggctctgg agcacatgac tccggttatt ttactgacag acggttttct ggccaacggc
     2521 tcctcggcct ggagattgcc cgatctcagc caattcccgg ccatcaatcc tccttttgtt
     2581 cacaaagaaa tgcaggggaa atgggctcct tatttacgta acgagcaagg ttcgcgctat
     2641 tgggctattc ccgggacaga gggctttgca caccgcatag gcggtctgga aaaagattcc
     2701 cgtacgggag ccatttccac cgatccggcc aatcaccaac tgatgacaga tttgcgtcag
     2761 caaaaaatcg acaatattgc caaagtgatt cctcctttgc aactgagcgg aaacagcaac
     2821 gccgatttgc tggtggtcgg ttggggaggc acatacggcc atttgtattc ggccgtgcgg
     2881 caattcaacg agcagggcag acaagccgcg ctggcccact tcaattatat caaccccctg
     2941 ccggccaata ccgaagaagt tttgaacaag ttcaaaaaga tagtggtctg cgaactcaac
     3001 tccggacagt ttgcttctca ccttcgctcc aaactgaaac tcaaaggaga aatacttcaa
     3061 tacaataagg tgcaggggca acccttcacc gtcgaagagc tgctccggca tttttcaacc
     3121 atactataac aagaaagtca tgcaaacatc ctatacagtc aacgatttta aaagcgacca
     3181 atacgtaaga tggtgcccgg gctgcggcga tcacgcgctg cttaattcta ttcagagagc
     3241 tatggcggaa atcggcgtct ctcccgacaa taccgcagtt atttcgggta tcggctgctc
     3301 ttcacgactt ccttactatg tcaacaccta cggttttcat accatacacg gacgagcttc
     3361 ggccattgct accggcgtaa aagtggcgaa tccccgtctg gccgtctggc aaatcaccgg
     3421 cgacggcgat tgtttggcca tcggaggcaa tcattttatt catgccgtcc gcagaaatgt
     3481 cgatataaac atagtgctgg tgaacaacgg tatttacggg ctgaccaaag gacaatacgc
     3541 ccctacctcc aaacggggct ttatttccaa atcctcacca tacggcacca ttgaagatcc
     3601 ctttaatgct tccaaccttt gccttggagc cggcggacaa ttcttcgccc gtattttgga
     3661 tactgacctt aaaaattctc aggaagtttt gaagcttgcc gcgcaacaca aaggctgttc
     3721 cgtggtggaa gccttggtca attgtgttat attcaacgac ggcacacaca aagaagtcag
     3781 cgccaaagag gttcgcgaag actacaccat tttcctgcgg cacggagagc ctatgatttt
     3841 cggtaaagac cgggacaaag gcctgattgt tgacggtttc aaactcaaag tggttaagat
     3901 aggcgaaaac ggcatcaccg aaaacgacat ccttgttcac gatgccaaat gtccggacaa
     3961 aactctgcac ttcaaactcg gccacatgag aggacccgaa ctccctctgg ctctcggcgt
     4021 tatccgcgat accgaagccc ccgtctacga cgaggaagtc tatactcaat tcgaagaaat
     4081 caaacctaaa cgcaattata agtccctgaa cgaatttttt atgagtctgg aaacctggga
     4141 aatcaaatga aactgcgaca actttgcctg attggtttat gtttctggtg ttttaacctg
     4201 aaatcggaac agcccgaaac ttacacgccc tacggcctgc aaaacaatat gcccttgttt
     4261 taccgacagg ccaaagaggc aatcactttt tccatggcct gggaacacca gggtaacgca
     4321 aaacaacttc agcaaaagaa acgcttcacc aaatggaaaa aacaagcccg gcaggtactt
     4381 ttcgattgca tgggtccgct cccgcctcca gcccgagatt tcgacgctct ggtgttggcc
     4441 ggggagcaaa gggagggcta cgaaggtgga aggccttacc tggctcaaaa aatacttctc
     4501 aatattacct catttgagcg cattcctgct tacttgctta tccccgaggg agaaggcccc
     4561 tttccggccg tcgttatgtt gcacgaccac ggtgcgaagt tttccatagg caaggaaaaa
     4621 atggtcaaac cgatcaatcc ctccgaagaa ttggaaaaag aatgcaatca atggtcggtg
     4681 caatgttacg acagcattta tacgggcgat tattttgctt cacagggtta tgtggtgctg
     4741 gccatcgatg ccttgttctg gtccgaacga ggttcggtcg aaggtcacga ttacaacaga
     4801 cagcaagcac tggcttccaa tatgtttcaa ctgggcagaa cctggtgcgg acacatcacc
     4861 aacgacgaca ttcagagcgt ggaatatttg tgcagccttc cctatgtaga catagaacgt
     4921 atagccgcct taggcttttc catgggcgcc caccgggcct ggatgctggc ggccgccagc
     4981 gacaaggtca gcgccgctgt tgccgtctgc tggatgaata ccaccgaata cctgatgacg
     5041 ctggacaaca accaaaacaa gggaggcagt gcctattcga tgttgctgcc cgaaatcagg
     5101 aattacctgg attatcccca tgtggcttcc attgcctgcc ccaagcctat gtatttcatc
     5161 aacggcagcc gcgataagct cttccctgta caaggttgtc tggatgcttt cgaaaccatg
     5221 cgccgggtct gggaaagccg gggagccgga gaccggctca agacagaaat ctgggattcc
     5281 cctcatttct tcagccgggc catgcacgag caaagtttgc tatttttgaa caaattcctg
     5341 cgaaaacact gagaacctga gtcttagagt ctccagccac ctcaactacc tctcaatcaa
     5401 gcccggacaa tattttcgat ctctacctga ggatgtaccg acggatcgac tgctgaaaac
     5461 attcggcatc gaagggatca atcccgtcgc tgacgaaata agaaaacgaa agaggggaaa
     5521 aataaaaaat attttcatcg attgctgaaa tttttcttta attaattagc agcatctgat
     5581 tttattttct atctttgcgc ccgataaaat ttggccctat aaaaagtttt caacaaattc
     5641 actattaatc atttaaaaat gaaatcatta gatctaacaa agtacggaat caaaggtact
     5701 accgagattg ttcacaaccc ttcctatgag caattattca aagatgagac cgatcccaat
     5761 ttacagggat tcgagagggg acaggttaca gaaatggggg ctgtcaatgt gatgacgggt
     5821 gtctataccg gacgctcacc taaagacaaa ttcatcgtga tggacgatgt ttcgagggac
     5881 accatctggt ggacttccga cgaatataaa aacgacaata aacccctgcc caaggcatcc
     5941 tggggtgtgt tgaaagacat cgttaccaag caattatcca acaaaaaact ctatgtggta
     6001 gacactttct gcggcgccaa cgaagccagc cgtcttaaag tccgttttat catggaagta
     6061 gcctggcagg cccacttcgt aaagaatatg tttatccgtc ctaccgaaga agaactcaaa
     6121 aattacggcg aacccgattt tgtatccctt aacggatcca aaacctgcgc cggcgctcaa
     6181 tacaaagaac tgggattgaa ttccgaaact ttcaccgttt ttaacctgac cgaaaaaatg
     6241 caggtaatcg gcggaacatg gtacggggga gaaatgaaaa aaggcatatt caccctgatg
     6301 aactactacc tgcctctgca ggggatagct tccatgcact gctcagccaa cgtgggcaaa
     6361 gacaacgata ccgccatctt cttcggcttg tccggtaccg gcaaaaccac cctttctacc
     6421 gatcccaaac gtcagctcat cggcgacgac gaacacggct gggacgacga aggtattttc
     6481 aacttcgaag gcggctgcta cgccaaaacc atcaatttga gcaaggaaca ggaacccgac
     6541 atctggaacg ccattcgtcg cgatgccctg cttgagaacg ttacggttga tgccaacggc
     6601 aaaatcgact ttaacgacag cagtgttaca gaaaacaccc gtgtttctta cccgatttac
     6661 cacatcgaca atattgtaaa gcccgtatcc aaagccggtc ctgccaagaa agtaatcttc
     6721 ctgtcggccg acgcattcgg cgtattgcct cctgtatcga tacttacccc cgaacaggcc
     6781 caatattact tcctgagcgg tttcaccgcc aaactggccg gaaccgaacg tggtattacc
     6841 gagcctactc ccactttctc tgcctgcttt ggagcagcct tcctggcctt gcatcctacc
     6901 aagtatgctc aggaactggt taagaggatg caagcttccg gagccaccgc ctacctggtt
     6961 aataccggtt ggaacggcac cggcaaacgc attaccatca aagatacccg cggcatcatc
     7021 gacgctatcc tggatggctc catcgacaaa gcagccacca aagacatagc ttacttcaac
     7081 cttaaagttc ccaccgcttt gcccaacgta aacccggcca ttcttgaccc cagggatacc
     7141 tatgccgacg gtaaagtatg ggacgaaaaa gccaaagacc tggcctcccg cttcatcaag
     7201 aacttcgata agttcaccaa aaacaacgaa gccggtcaaa aactggtagc tgccggtccg
     7261 aaactttaag aagagattct ttcttttagc ttataattaa atagggcgct gcgttataaa
     7321 cgcggcgccc tattggtttt taaatgttct aaacttgctc tgcctttgag gccactattg
     7381 cggctccggc agcacttcca gaccctgtgt cttatgacct ggctctgcct ctgttaatcc
     7441 taccggccgg aaacttccaa aatacgacaa aaatcaatct attatatcaa agcccgtata
     7501 aggacgcaag acctcgggaa tacgaattcc ctctgccgtc tggttgtttt cgagcaaggc
     7561 agccactacg cggggcaagg ccagcgcact tccgttgagt gtatggcaca actgaatttt
     7621 acctccctcg gcagtacggt aacggcattt gagcctgttg gcctgaaaac tttcgaaatt
     7681 cgacaccgag ctcacttcga gccagcgttg ttgggcggca gaaaagactt caaaatcaaa
     7741 agtcagagcc gaggtaaagc ccatatcgcc cccacacagg cgcaaaatgc gccagggcaa
     7801 ttccagcgct tcgaccagac tttgcacatg ggtaaccatt ttttccaaac gttcgtaaga
     7861 acgatcggga tgttctatgc agacaatttc caccttatcg aactgatgca ggcgattcag
     7921 tcccctgacg tccttgccgt aagctccggc ttcgcgacgg aaacaggccg aataggcggt
     7981 attctttacc ggcagatcct tttcgtccaa aatgacgtcc ctgtaaatat tggttaccgg
     8041 tacttcggct gtcggaatta agtacagatc atccaacccg acatgataca tctgcccctc
     8101 tttgtcgggc aattgccccg taccataacc cgaagcggcg tttaccacat aaggcggttg
     8161 aatttcgagg tatcctgcct gccgtgcacg atcgaggaaa taatttacca gagctctttg
     8221 caaacgggct cctttgcctt tgtaaaccgg aaaaccggct ccggtaattt tggctcccaa
     8281 ttcaaaatcg atcaaatcgt attttttggc caggtcccaa tgaggcatgg catcgacggg
     8341 caagtcgggc atacggccgc cggaacgttc cgtcacattg tcttcggctc cttttccggc
     8401 cggcactgaa ctatgcggaa gattggggat ttgcactaag agttcctgta ttctgttttc
     8461 ggcccctttg cgcgcagttt ccaaactttt accttcttct ttgagttgca gggtcttttc
     8521 acgtaccacg gcagcttcct gttttttgcc ttcctgcatc aacacgccta tggatctgga
     8581 aagagtattc aattctgctg cgcaggcatc cgaccgggtc tgagcattgc gccgttgcaa
     8641 atcgagctca agaatttctc caatgagcgc ttctgcatca aaatgtttaa cagccagacg
     8701 ctctatgcat tctctggtgt tttcgctgag gtactttagt gtaagcattg tttcgatttt
     8761 atataaacaa aaggcacttt aaagcggaaa attctacgtt taagtgcctt ttggtcggga
     8821 tgagaagact cgaacttccg acctctacgt cccgaacgta gcgcgctacc aactgtgcta
     8881 catcccgatt gaaatcaccg tgcaaaggta gaaaagcttt ttcccatgta caaacaaata
     8941 gcccgttttt tacaaaataa ttcggcaaag tctttgttgt tttaaaaatg aattgtactt
     9001 ttgcagccgc ttccaaaagg aaaaggtacc atagctcagt tggtagagca aaggactgaa
     9061 aatccttgtg tccccggttc gattcctggt ggtaccacag aaaaaacccg cttttcaaga
     9121 gcgggttttt ttattcctga tcggctccgt agaaaagtgg caattgattc gcaacttatc
     9181 gatgaaacaa ctataaatca ggatttttaa atctttgttt ttaaaaataa agtcttttac
     9241 cgtatcttgc aacacgaaga ttcttcctct aaaagcatgc ataataaatt tatcccatat
     9301 tgtttctttt tactgcttgt tgcaggagca ttccggttgc aggctgaaac aggcagagag
     9361 ctctggttgc cggcaactgc cctgtcgaca gtcaaagtct atatgccgtc cggaaaaagc
     9421 tctcctactc tggagctggc caaacaagtc ttgatcgagg gctggcagga tcgggaaaac
     9481 aacagcatca aattaaagat cagtcgcgat aaaagactgt ccgaacaagg ttacagttta
     9541 tcgcccggct gcatcacggc caaaacacat caaggtttgc tctacggagc ctatgaatat
     9601 ttgcgacgcc ggcaaatcgg tctcagttta gaactgccct tttccaatcc ctcctaccaa
     9661 aggcgcttac ttaaccattg ggacaacctg gacggaagcg tcgaaagagg ctatgccggc
     9721 ccttccatct tttggaaagg taagcagtcg atggagccga cggcagaaga ccgcgaacac
     9781 tggcgaacct acgccgcaat aaatgcgtct atcggcatca acggggccgt tttaaacaac
     9841 gtcaatgccc cgcccgaagc cctgagcctg cccgtactaa aacgcgctgc agctatcgcc
     9901 gacgagttgc gcccctacgg aatggtgagc tacctgagca ttaatttttc tacgcctatg
     9961 cgtctgggcg gactcaaaac ggccgatccg cttgactcgg aggtggtaga ttggtggcgt
    10021 gcaaagatcc atgaaatcta ctccctgatt cccgatttcg ggggctttct ggtcaaagcg
    10081 agcagcgaag gcatgcccgg gccgggagat tttggacgta gccacgccca gggagccaat
    10141 atgctggccg aactgctcaa accctaccac ggcatcttaa tgtggcgggc tttcgtatac
    10201 aaaccgagcg acaacgatcg ggccaaacaa gcctacgagg agttcatgcc tttggacgga
    10261 caattctgcg ataatgtcgt tgttcagata aaaaacggac ccatcgattt tcagcccaga
    10321 gagcccttca gtcctttgtt tggagccctc aggaaaacgg ccgtcctgcc cgaactgcaa
    10381 atcacccagg aatacctggg acaagagcat caattggctt acctgggcgg tttatgggaa
    10441 gaattcttgc aaagcgatac ctatcagtca ggcccgggca gcaccgtagc ccgctgtacg
    10501 gacggcagtc ttttcgatca atccctcact gccatagccg gggtttcaaa catcggttcg
    10561 gacagcaact ggtgcggcca tccgtttgcc ggcgccaact ggtatgcttt cggacgtctg
    10621 gcctggaaca accgggcctc agccgccggg attgccgaag aatggttgag gctgactttt
    10681 gaacccacgc aatccccgga aggcaattcg ctttcggacg aaggtccggc cttggacaac
    10741 gatccggctt cggaccaaaa gaacggcaac tgggaaaagg aatttcttca accggtttta
    10801 tctatgatgc tccggagtcg cgaggcgatg gtcaattaca tgatgcctct gggcctgcac
    10861 cacttgtttg ctctcgacca tcactacggg cccgggcctt ggtacgactc cccgagacaa
    10921 agaaaagact ggactccgcc ctattatcat caggccgatg ctaaaggcat aggctttgac
    10981 cgcagttcgg ggggcagcaa cgccgtgtcg caataccggg agcccttgcg ttcccaattc
    11041 gacaacccgg gcagctgtcc cgaaaatctg ctgctctggt ttcatcacct cccctgggaa
    11101 catcgcctgc ggaacggcaa cagtctgtgg gaggaactct gccggcgcta cgatgccggc
    11161 cttcaggaag tccgccgttt ccaactgatc tgggataaca tggaaaaccg ggttgacagc
    11221 gggattttta ttcaggttca agccaaactc cgcagacaag cccgggatgc ccaggtgtgg
    11281 aaagacgcct gcctgctcta tttccaatcg atcaaccaac tggctttccc cgaagatatg
    11341 gaaaggccgg ttcacgacct ggaagtactc aaaaaaacgg atatgcggta ttttatgaac
    11401 aggtagacta accataaaaa tctttcgaac aacagataaa agactccata caaccatact
    11461 aattgccaaa cttcttagcc ttgaaaaata tgcaacgaac attcctccta ctgactacag
    11521 ttgtcctgtc gttttgcagc cacttaacgg ctcaaacgac tctgcgccag gccacccaaa
    11581 accttttttt gataggcgta gccgtcaaca accctcaaac ccggggtgcc aggccggccg
    11641 aagcagaaca gatatccagg cacttcagcg ccattgtgcc cgaaaactgc atgaaatcgg
    11701 cggagattca tcccgaagaa catcgttaca acttcgaagc caccgaccaa atgctcgctt
    11761 ttggagagaa caaccggcaa gtcgtcaccg gacattgtct catctggcat tctcagctgg
    11821 ctcgctggtt ctgcgtagat gaacagggtg agccggttag tgccgaagtt ctcaaatcga
    11881 ggatgcggga gcacatcttc agcatcatga ctcgctacaa gggccgcatc aagggctacg
    11941 acgtggtcaa cgaagctttc gaagacaatg gtacttaccg caacagcccc ttttatcgta
    12001 ttctgggcaa agattttatt cgactggctt ttgaatacgc ccaccaggcc gatccagagg
    12061 ctgaactcta ttacaacgat tacaacatgg ccaatccggt caaatgcgat gctgttgtca
    12121 agatggtaga agaactcaaa gccgccggtt gtcgcataga tgctgtgggt atgcagggac
    12181 acgtgcacat ggacgaacct tcggtggagg ctttcgaaac aagtatcctg aaactggctg
    12241 ctaccggcgt caaggttctg attaccgaat gggacatttc catcctgcct aatccctacc
    12301 ggcacacagg ggccaatatt gccgaccgct ttgcctattc tacccaaaac gacccttacc
    12361 gcgagggaat ccccgacgag gtgatgaaag cctgggaaca acgtgtcacg gagctttttg
    12421 ctctctttct gaaacaccac cggcacatcg atcgcgttac actctggggc ctgaacgacg
    12481 gcaattcctg gcgaaacaac ttccccgtcc gcgggcgcaa agactatcct ctgcttttcg
    12541 atcgcgacaa ccaacccaag ccggtagtac aacaaatgat tgaattggcg ctaaaagcta
    12601 agacgaagca gtgattttct ttatttcttt aaaaatacca ttaacacccc ttgttgctgt
    12661 tcacgaattt gctataaata gggacgatat tgacaacaat ccgttttttg ggccaagata
    12721 ttatctctaa ttttggcagc agggatgtgt tgtccctcat ttttattttt taaaacataa
    12781 atttcttcat actcaataac aaccatgaga atccccaaac ttattctcgg cttttgtgcc
    12841 ttattcacca ccgccttaac ggcccaaaac ggtgatcaga ttttggacgg tataggagaa
    12901 accggcctaa ttgcccggta tacatttaaa agcaatgccg acgactggtc gcgcaacaat
    12961 ttacacggta atatcaaagg agcgggctat cgttttgcga cagattccct ttttagcacg
    13021 gtattatcct tgccggaaga agctgaaagc tatgtaatta tacccggaga atctgtatcg
    13081 ggcgaagaat cgctcagtat cagcgcctgg atttatccgc gttctgccgg ggagaaacag
    13141 gtgtttttcg atttcggcaa gaacggccaa tcacgccttc tggtaactct gacaggcagt
    13201 ggggaaaaaa caaccattct ggctcaggtg cagactcctg ccaaaaccta tgaattgagc
    13261 tctgaagccc tggctataaa cagctggcac cacctggccc tggtcataaa tctgccgtca
    13321 aaaagcctga atatgtttat caatggcaaa atagccaaag aggtacaaaa agtagaactg
    13381 agtctggcgg atttgttcga catggaaacg ggaaaagaaa acaaactcct gatcggaaaa
    13441 tcgcaatctc cggacaacaa acccttgaat gcagctttgc atgattttcg tctttaccgt
    13501 attccgctga acgagtggca gatagccagg atatacggca ttgccctgaa caaggaagaa
    13561 ccgatagtca gaagaaggag aacggaagcc aaccgggaac tgcccgtatt ttcccccacc
    13621 actcctcagt tgtataatgc cttcttaact gctgtacccg acatcgaagt agaaacagtg
    13681 accggacagt tgcctcgcct gccacgttat ttaaaaggag tttacgaaaa gggtatagaa
    13741 ggacccgatg tcagagtgat ctggccctca ccaaccgata attcacaggt acttaccccc
    13801 ggaacatacg aagtaaccgg caaagtttcg ggcaccgatc ttcagcccaa agccctggtt
    13861 accgtaaaga cggccgaaaa acaaaatggc ccgcaacgca aagtagaagc ctttcaattg
    13921 gaccgggtaa cgctgaatac ggattgtcac ggtcgtaata ccaaattcat cgagaatcgc
    13981 gataaatttt tcaacggcct gggcgccacc aatcccgata attttctcta catgttccgc
    14041 aacgcctttg ggcaacaaca acctgagggc gctgaacctt tgggtgtatg ggacagccag
    14101 caaaccaaac tgcgcggaca cgccacgggg cattacctta cagcactagc tcaggcctat
    14161 gcaggcagtg cttacaataa agacttacag gccaactttg ctcaaaaaat ggactacatg
    14221 gtcgatgtcc tttatgaact ggctcaactt tcggggactc ctcaaagccc tggaggaacc
    14281 tgtgtcacgg atccctgtca ggtgccgccc gctgaaggaa aggcaagcta tgactccgat
    14341 ttaagcgaag gaggcatacg caccgattat tggaactggg gcaaaggctt tatcagcgcc
    14401 tacccgcccg atcagtttat ccttcttgag cagggagcca cctacggagg ttccaattct
    14461 caggtgtggg caccttacta caccctgcat aaaattctgg tcggtctgat ggatatttat
    14521 gaaatctgcg gaaacaaaaa agccctcgat gttgtgacag gcatgggcga ctgggtacat
    14581 gcccgtttga gtaacttacc caaagaaacc cttatcaata tgtggagccg gtacatagcc
    14641 ggcgaattcg gaggtatgaa cgaagccatg gccaggttgt cgcgcctcac aggcaaaaaa
    14701 tcgtatttgg aaaccgcccg cctgtttgat aatataaata tgttttacgg taacgcggaa
    14761 cattcgcacg gactggccaa aaacgtaaac agcttcaggg gttttcatgc caaccaacac
    14821 attccacaga ttgtaggagc gctggaaatc taccgcgatt cacaagctcc cgaatacttc
    14881 tgtattgccg ataatttctg gactatggtc accggcgatt ttatgtatag catcggaggc
    14941 gtagccggag cccgtaatcc caacaacgca gaatgtttta ccgcccagcc ggcttcactc
    15001 tacgaaaacg gttttgctgc cggcggtcag aacgaaacct gtgccaccta taatatgctc
    15061 aaacttacga gtggtctgtt tatgttcgat caacgggcag aattgatgga ttactacgaa
    15121 cgcgggcttt ataaccatat tttggcttcg gtggccgagc atagtccggc caatacctat
    15181 cacgtgcctt tgagacccgg ctccgtgaag caatttggca acccgcacat gaacggattt
    15241 acctgttgca acggtaccgc gatcgagagc agcaccaagt tgcaaaactc tatatatttc
    15301 aaaagtaccg acaacaaaac cctgtacata aatctttacg tagcatcaac cctgcaatgg
    15361 aaggagcgta aaatcaccat cactcaaagt actgcttatc ccaacgaaga ccaaaccaaa
    15421 ttgaccatac agggcaaagg caaatttgaa ttgaaggtaa gagtgccgcg ttgggcaaaa
    15481 aaaggcttct ttgtaaaaat caacggtaaa gaaatcgagg tcgatgctca gccgggtact
    15541 tacctgagcc tgaatcgtaa gtggaaaaaa ggagatgtcg tggagttgcg tatgcctttc
    15601 agcttttatc tggaaccggt aatggatcaa caaaacattg ccagcttgtt ttacgggcct
    15661 gttctgctgg ccgcccagga agaagaagcc cgcaccgact ggcgcaagat cactctcgac
    15721 gcaaaagaca tcggtaaatc gataacaggc gatccttcca gcctggaatt caagatcggc
    15781 gatgttttgt tcaaaccatt ttacaacact tacggacgtc attccgttta tctggatgtc
    15841 agcctaaaat agcctttaag cttataatag accctttcag tcctaaccct taatatttta
    15901 ctcaaaagca agttccgaaa aagttcggaa cttgcttttg gctttatgga tggcgaattc
    15961 gcaacatacc tctataaaaa acaatcgaat tgtttttttt ttggatataa catagggcta
    16021 ttcgcatttt tttgtatcat tgtagtctga ttgttatttc tgttgtaatt atcaaaaaga
    16081 tt
//
LOCUS       DAOSGL010000013        15420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_3143_length_15420_cov_4.780735, whole genome shotgun sequence.
ACCESSION   DAOSGL010000013 DAOSGL010000000
VERSION     DAOSGL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3143_length_15420_cov_4.780735"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1562
                     /locus_tag="PKY68_00905"
                     /pseudo
     CDS             195..1562
                     /locus_tag="PKY68_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
     gene            1536..2855
                     /locus_tag="PKY68_00910"
     CDS             1536..2855
                     /locus_tag="PKY68_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006849134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH23630.1"
                     /translation="MPEPFTFDWWQFNSHANLPIIQTSFTADPAPVVINDTVFLYTTH
                     DEDGARGFQMFDWLLYTSDDMVNWQDHGAVASLDDFKYYDGKNGAWAQEVVEANGAYY
                     MYCPIHGRGIGVLMSDSPYGPFKDPLGEPLVWQKEHWNDIDPTILIDDDGQVYMYWGN
                     PDLYSVKLGNDMISLAGPIVKHPKIQDYQEGPWIWKHEGRYYLAFASTCCPEGIGYAM
                     SDGPEGPWEYKGHIMDHTVRTRGNHPGIISYKGKHYVFGLNYDIMHLTTFAHAEQRSA
                     ALAEMHYNPDGTIQEVPYFMDNVVEQIKPFDPYRRVEAETMSWGYGLKTKRMDEENKN
                     STNMFVTNIDDGEHIKVSGVKFGNKGARKFYASVATDSLARIEIRLDSPDGPLAGTLP
                     ISPTGGESQYHTVSCKLKPRLRGTHDLYFVFRGTGKNLFNFDWWRFK"
     gene            complement(2894..3466)
                     /locus_tag="PKY68_00915"
     CDS             complement(2894..3466)
                     /locus_tag="PKY68_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOH23631.1"
                     /translation="MQLKGSKTEQNLLKAFAGESQAKTRYEFFAGVAKKEGYEQISAF
                     FTETALNEKEHAELFFKYLEGGMVEITASYPAGVIGTTAENLKAAADGEHEEWAELYP
                     LFAQIAKEEGFKDISATFTLVAKVEAEHEKRYRKLLQNIVENKVFEDDENTSWTCRNC
                     GYVHKGKNVPDLCPVCKHAKAYFERRCENY"
     gene            3784..5430
                     /locus_tag="PKY68_00920"
     CDS             3784..5430
                     /locus_tag="PKY68_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH23632.1"
                     /translation="MSKHFFSFLEDTIKNHWDAPAITDYETDFTMTYGELAEHIAQLH
                     LLFKEMGLQKGEKIAICGRNSVNWAMSFLAANTYEAVVVSILPDFHVDSIHALVNHSE
                     ASLLFVGPSVWENLEAEKMPQLKSIVSLRNFELIQTKSAAVKQAFAIKDQLFGRHYPN
                     GLQMQDISYPKNNFDDLAVINYTSGTTSAPKGVMLTHRSISSNVEFGQARIPNQVGWN
                     IVSMLPLAHMFGLTFEFLYQLAGGCHVYFLNKTPSPQVLMKAFADTNPYMILTVPLVI
                     EKIFKKTVFPVIHKPVMKVLWYTPGINLVLRNAVRKKLMTAFGGKLRYLIIGGAALNF
                     EVENCLKQIRFPYTVGYGMTECGPLLGYEDWRHFAKASCGKPVHRIELRIDSANPYKE
                     AGEIQVKGDNVMLGYYKNDEATEAAFTQDGWMRTGDLGLLDKRGNIYIKGRSKSMILG
                     SSGQNIYPEEIEDKLNNLPLVVESVVVERDEKLVALVYPDFDAAGGESKEEAINEIME
                     QNRLSLNKLLPAFARIMKIELVKKEFEKTPKRSIKRFLYK"
     gene            complement(5460..6581)
                     /locus_tag="PKY68_00925"
     CDS             complement(5460..6581)
                     /locus_tag="PKY68_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HOH23633.1"
                     /translation="MNLAQRQEELQRQFKLGIDTKLENSPVEIRHFPYTSLDEDKGEK
                     ARGSDSDILENREFVYPIFKLKSQDKCPGAIFLMHGLNERSWNKYLCWAEYLCMHTGK
                     AVVLLPIAFHINRSPLSWLNPRLISPILEKRRQILGKEQSQSIANTILSERLSEDPKR
                     FYRSGKQSLSDLTGLVRQIKEGRHPLFTSGTGVDFFAYSIGAFLVQIALLSDKEGLLE
                     DSGLFLFCGGSIFKQMNGTSRFIMDKLTFDKLCDYYLYRFESDNRIDQTSEASMIAFN
                     TMIEKERGAEERYRIFNEMGNKIQVMALKKDSVIPYNGIVEALGTECAQRHSSLIDFP
                     YEYSHEQPFPIIKGQKEQNDLVNLCFNGVFSQAAAFLSK"
     gene            6786..7643
                     /locus_tag="PKY68_00930"
     CDS             6786..7643
                     /locus_tag="PKY68_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOH23634.1"
                     /translation="MDRKIPDYINEKGNVVRLVVLTAVFALLFINIYEPFGSKNWQGG
                     VSDLTYFLFSNLVILVGMCVVAISRIIMYHYNKNHEISVLLYLAWVVMEVIAMSLFYT
                     LFQFFALNDTRPFLEIWKNATINTSLILLLPYAILWLYFSWRDNEKMLKRIREQESLL
                     AEKQKSGLISFYDDKGVLRLSVDINSLYYIESADNYVKIHYLNKGKMQHFMLRSSLKT
                     IDDLFGDKYLVRCHRSYIVNFDKIKILRKSEEGLSLELDSDLVPDIPVSKTFSNKLMS
                     RFTDLQKGS"
     gene            complement(7673..10678)
                     /locus_tag="PKY68_00935"
     CDS             complement(7673..10678)
                     /locus_tag="PKY68_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOH23635.1"
                     /translation="MTADDRLKKLDFKDTSFANLMVKRIFNILLISSKYDFFMLEDDS
                     RVDEQIFNEYISLNLRYPPRFTQVSNKKEALEQLGQNNYELIIYMPSDKSNEHFEIAK
                     QIKHKWPAIPIVVLTPFSREVSQYLANADTSAIDYVFSWLGNSDLLLAIIKLMEDKMN
                     VEEDVASVGVQVIMLVEDSIRSYSSILPNLYKYVLEQSRNFATEALNAHQRTLRMRGR
                     PKILLARSYEEAMEIHDKYKDYMLGIISDVSFMKEGKKTQNAGFLLCNEFRKYDKALP
                     VIINSSEASNRTYAEQNEMGFIQKSSKTYSKELRHLLADTFGFGDFVFKNPQTGEEVA
                     RIQNIQELQSAIFDIPDDSLRYHFERDHASRWLFSRAMFPIARFMKGILIQDYEDTAP
                     VRKMLFDAIVLYRRVKNQGIVAEFQKGRFDKYSNFARIGEGSLGGKGRGLAFLDVMLK
                     SHPELNTYRNVHIGIPKTLVLCTDIFDEFMDNNHLHELAMSDQTDEIILENFLKASLP
                     PRLLENLTAFVDVVQKPIAIRSSSLLEDAHYQPFAGIYSTYMIPYNEHAEDMLIMLKS
                     AIKAVYASAFFQQSKAYMQATSNLIDQEKMAIILEEVCGTAFDERFYPTFSGVARSLN
                     YYPIGEEEAGDGIANVALGLGKYIVDGGTTLRFSPKHPKHLLQMSSLNYALSETQTHF
                     LALDLKNKSFRLKVDDGQNLLELPISEAEKDGNLKFLASTFDPLDQVIRDGLWEGGRK
                     IISFAHILQNDVFPLADILSKILKLGEQEMGRPIEIEFAVDFHPRDEDDSPEQRYFYL
                     LQIRPIVDTKEMLDEDLDEIPEEQLLIKTISALGHGIVDDIYDLIFIKPELFSSSNNQ
                     LCVHEIEKINKQLGDEGRNYILIGPGRWGSSDSWMGIPVRWSQISNARLIVETGLRKY
                     QIDPSQGTHFFQNLTSFGVHYYSLPNSDMAYNYVDYEYIHNQPALFENQFLKHVRFEN
                     PLVFKTDGKKGKGVLLKANRLSVAFPKMS"
     gene            10803..12035
                     /locus_tag="PKY68_00940"
     CDS             10803..12035
                     /locus_tag="PKY68_00940"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOH23636.1"
                     /translation="MHPSEYIKESMEYRFIREHIKEDVRKLALQTHGMKDMNYPFAIR
                     QISGYQHIVNKIPDWYACPDILFPPTLSLEQCSSQQTACYKRDLMKTIIRKQKQEQTI
                     SGADLTGGFGVDAYYLSEVFDRFFYVEKQPELCRIASHNFKALNRPGIQVFENESEEF
                     LQQSGKLDFVYLDPSRRNRDGNKVTALRDCSPDPATLYEDLIKKAGLVLIKLSPMIDL
                     SLALKALPHTRQIHVVALDNDCKEVLFLLDAKYQTAEPEITAVNLKNNAADQVLKFRR
                     SDEANASCVYTAQVGDYLYEPNVSLLKAGAFIMPAQAYGLEKLHKNTHLYTSNKFKSD
                     FPGKIFEVKTISSAKTKDFHKKLPGLQQANLVVRNVPMSVEALRKKLNLKEGGEDFVF
                     ACTLADESKVFLYGKRLL"
     gene            12122..12649
                     /locus_tag="PKY68_00945"
     CDS             12122..12649
                     /locus_tag="PKY68_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOH23637.1"
                     /translation="MIENEKTTTIQAALEVARRMMTAARTAPKARGIDRLELCCVSGE
                     ELEILAKKMEEIGLKNRRPSFARDAQNIRQSQAVVLIGSRQGEQELDCAYCGFPNCAE
                     KRKHPAFPCVFPVTDLGIALGSACAVAADSRVDSRVMYTAGLAALELGWLKDCFYALS
                     IPLSISGKSPYFDRK"
     gene            complement(12786..14651)
                     /locus_tag="PKY68_00950"
                     /pseudo
     CDS             complement(12786..14651)
                     /locus_tag="PKY68_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(14883..>15420)
                     /locus_tag="PKY68_00955"
     CDS             complement(14883..>15420)
                     /locus_tag="PKY68_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOH23638.1"
                     /translation="HPAELKTDAVKKAIKQIRATGAQIRTQSPLLKNINDKPEIWAEM
                     WRKQVDLGCIPYYMFVARDTGSKRYFELPLDKCWNIFRKAYRQVSGLCRTVRGPSMSC
                     EPGKIQVLGVQEVKGEKVYVLRFLQGRNAKWVDIPFFAKYDPEATWFDQLEPAFGEEK
                     FFFEKGRRRPKTNEILFE"
BASE COUNT         4071 a         3340 c         3662 g         4347 t
ORIGIN      
        1 gaaatcgctc ccgatttcag ccggttggag cagaagggta aggtgacggt atttaccgac
       61 aggggtcagg aacccggtca gggtttcgag atcaatgaga atgtcgacct gcccgaaact
      121 tttattttgc agcctcgcgg cggatttgta atcttgactg attaaagaat tactaaactg
      181 aatgatataa acctatgaga accttaaaac tattattgac ttttacagtc gctttgacgg
      241 taccggtttt tgcccaaaat ccgataataa gcacgatgtt tactcccgac cctgcgcctt
      301 tcgtatatgg tgacaaggtc tatctgttta ccgaccatga tgaggacgga cacaatacgg
      361 acttcaacat gaaagactgg ttgttgttca gcaccgagga tatggtgaac tggacctatc
      421 tgggcacaca ggtcacaact gccactttct cgtgggcaag acagggcaag atggcttggg
      481 cctcacaagg tgtggagcgc gacggcaagt ggtactggta tgtctgctgc aatatagccg
      541 gcggcggcga tgccctggct gtggctgttg cagacgatcc tcaaggccca tggatggatg
      601 ccataggcaa gcctctggcc gagggattcg gttttattga ccctacggta ttcattgacg
      661 atgacggtcg tgcctatctt ttctggggca ataaaggtct ttggtacggt gaactgaacg
      721 atgatatggt ctcgtttaag gatggatgga aagaggtgcc cggctatcgt gatccgaaat
      781 ctttcggtga gttgcaatcc aaaatgaatt gggcgcgcgg ccgtaatgaa atgatgaccc
      841 agtttgagga gggaccgtgg cttatgaagc ggaacggtat ctactacctc tcttatccgg
      901 caggcggcgt acctgaacat atggcttact cgacagcgcc taccgtgcat ggtccctgga
      961 cctatcgggg ccgtatcatg gatgaggcag aaaacagttt taccattcac ggaggaaata
     1021 taacgttcaa gggacgtgac ttcatgtttt atcataacgg catgctcccg ggaggcggcg
     1081 gctttaaccg ctccgcctgt gtagaggagt tcaagttcaa tgaggatggc tcaattccgt
     1141 tcatcccctt cacaaaagag ggtgtggtaa caccaatcaa gaatcttgac ccatataaac
     1201 gtgttgaggc cgaaacgatg gcagaggcac gcgggctgaa gaatgaccgt cttgccggca
     1261 aggatcacta tgtgactgat atacacgatg gtgactggat gcgcgtgcgc tcggttgatt
     1321 ttaaggattg cggtcagaaa cttgttaccg tggttgtggg aaggcagaga ggcaagggaa
     1381 ccattgagtt ccatatagac aatatggaag gtgagccgtt ctgcactgtt ccggtaaata
     1441 cggacaatgc gggatttccc actgcagcct tctcgtacct gattgacaca aacgtcaccg
     1501 gtgtacatga cctgtatatt gtgttccggt gcgagattcc cgagcctttc acctttgact
     1561 ggtggcagtt taactctcat gccaatctgc ccataataca gaccagcttc actgccgacc
     1621 ccgcccctgt ggtgatcaac gacacggtat tcctctatac cacacacgat gaagacggcg
     1681 cccgcggatt ccagatgttt gactggctgc tttacacatc ggacgatatg gtaaactggc
     1741 aggaccatgg cgctgtagct tcgctcgatg actttaagta ctatgacgga aagaacggtg
     1801 cgtgggccca ggaggtggtc gaggccaacg gagcatacta tatgtactgc cccatacatg
     1861 gtcgcggaat aggcgtactt atgtccgatt cgccatacgg accgtttaag gatccgctgg
     1921 gagaacctct ggtatggcag aaagaacatt ggaatgatat tgatcctacg attttgatcg
     1981 atgacgacgg tcaggtatat atgtattggg gtaaccccga tctctattcc gtaaaattgg
     2041 gcaatgatat gatatcgttg gccggtccta ttgtcaaaca tcccaaaata caggattatc
     2101 aggagggacc ctggatttgg aagcatgagg gacgctacta tctggctttt gcatcgacat
     2161 gctgtccgga aggtatcggt tatgctatga gtgacggtcc ggaaggaccc tgggagtaca
     2221 aaggacatat aatggaccat acggtacgta cccgcggcaa ccaccccgga ataatctcat
     2281 ataagggcaa acattatgtg tttggcctga attatgatat tatgcatctt acaacatttg
     2341 cccatgccga gcagcgttcg gcagcattgg ccgagatgca ctataatccc gatggcacca
     2401 ttcaggaagt tccctatttt atggataatg tggttgagca gatcaaaccg tttgatccct
     2461 atcgtcgtgt agaggctgag accatgtcct ggggctatgg cttgaaaacc aagcgcatgg
     2521 acgaggagaa taaaaactct accaatatgt ttgtcaccaa tattgatgat ggtgagcaca
     2581 taaaggtgag cggtgtaaaa ttcgggaaca aaggcgccag gaagttctat gcttcggttg
     2641 ccaccgacag tctggcccgc attgaaatac gtctcgacag tccggatggt ccgttagcgg
     2701 gcacactacc catatcacct accggcggtg aatcacaata tcacacggta agttgtaaat
     2761 tgaaacccag attgcgcgga acgcacgacc tctattttgt cttcagaggt acagggaaga
     2821 atttgtttaa tttcgactgg tggcgattca agtaagaata gccacagact gatttatacc
     2881 aaattcagcg cttttaataa ttttcgcacc ttctttcgaa ataggcttta gcgtgtttac
     2941 aaacggggca gagatcggga acgtttttgc ccttgtgcac gtagccgcaa ttgcgacagg
     3001 tccaggaggt gttttcgtcg tcttcgaaca ctttgttttc gacgatgttt tgcaacaact
     3061 tgcgatagcg tttttcgtgt tcggcttcca ctttggccac cagagtaaaa gtggcggaaa
     3121 tatctttgaa accttcctct ttggcaatct gtgcaaacag aggatataac tcggcccatt
     3181 cttcgtgttc gccgtcggcg gcagctttca ggttttcggc agtagtgccg attacaccgg
     3241 cgggatagga ggctgtgatt tcaaccattc ctccctcaag atatttaaag aacaattcgg
     3301 catgttcttt ttcgttgagg gcagtttcgg taaagaaagc gctgatttgt tcgtaacctt
     3361 ctttttttgc tacgccggca aaaaattcat aacgggtttt agcttgagat tctcctgcaa
     3421 aagctttaag caggttttgt tctgttttgg aacctttaag ttgcatagtg ggtaatagtt
     3481 agattattaa aatatgattc aaattgagag gcacaaatat agcaaataat tggaatatgc
     3541 tttgataatt tcatttaggg aaggcttttt caagcctgca gtcgcagaac tttcgtccct
     3601 aaatagggca tttggcgtac acatttgtgt tttcgtccca gttttgagat ggcatagccc
     3661 atttttttta aatgcccgcg cacgaggcta cttttgcccg gcaaaaaatt tcaaagggaa
     3721 aaccttgaac cggttaaccc tgatagtgaa cctaacttca aaaaacaaaa ttaatttacc
     3781 gcaatgagca aacatttttt tagcttcctg gaagatacca tcaaaaacca ttgggacgct
     3841 ccggccataa ccgactatga aaccgatttc accatgacct atggcgaact ggctgaacat
     3901 atcgcccagt tgcatttgtt gtttaaggaa atgggtcttc aaaagggaga gaaaattgcc
     3961 atctgtggca gaaacagtgt taactgggca atgtcctttt tggcggcgaa tacctacgaa
     4021 gccgtggtgg tgtctatttt gccggatttt catgtagaca gcattcatgc attggtgaat
     4081 cattcagagg catcgctgtt gtttgtggga ccctcggttt gggaaaacct cgaagcagaa
     4141 aaaatgcccc aattgaagag catagtgtcg ctcagaaatt ttgaacttat acaaaccaaa
     4201 tcggctgccg taaagcaggc ttttgcaatc aaagatcagc tttttggccg gcattatccc
     4261 aatggtttgc aaatgcagga tatttcttac ccaaagaata atttcgatga ccttgcggta
     4321 attaattaca cttcgggcac caccagtgca cccaaaggcg taatgcttac tcacagaagc
     4381 ataagctcca acgttgaatt tgggcaggca cgtattccca accaggtggg ctggaatatt
     4441 gtctccatgt tgcctttggc gcatatgttc gggcttactt ttgagttttt gtatcaactg
     4501 gccggcggtt gccatgtgta tttcctgaat aaaactcctt cgcctcaggt tttgatgaaa
     4561 gcctttgcag ataccaatcc ttacatgata ctcactgttc cgctggtgat agaaaagatc
     4621 ttcaaaaaga ccgtcttccc cgtcatacac aagcctgtta tgaaggtgtt gtggtatact
     4681 cccggcatca atcttgtgtt acgcaatgcg gtcagaaaaa aattgatgac ggcttttggc
     4741 ggcaaactta ggtatttgat tatcggaggt gccgctttga actttgaggt tgaaaattgc
     4801 ctgaagcaga tacgtttccc ctatacggtg ggctacggca tgaccgaatg cggccccttg
     4861 ctgggttacg aagactggag acattttgca aaagcttctt gtggaaaacc ggtgcatcgg
     4921 atcgaattgc ggatagactc ggccaatccg tacaaggaag cgggggaaat ccaggttaag
     4981 ggcgacaacg tgatgttggg gtattataaa aacgatgaag ccactgaagc tgctttcact
     5041 caggatggct ggatgcgcac cggcgacctc ggtttgctgg acaaacgggg aaatatttac
     5101 attaagggtc gaagcaaaag catgatttta ggttcgtcgg ggcagaatat ttaccccgaa
     5161 gaaatcgagg ataaattgaa caatttacct ctggttgtag aatcggtggt agtagaaaga
     5221 gatgaaaagc tagtggcctt ggtttatccc gacttcgatg cggccggagg agaatccaag
     5281 gaagaagcca taaatgaaat catggaacaa aatcgtttgt cactgaataa acttctgccg
     5341 gcattcgccc gaattatgaa aatagagttg gtaaagaagg aatttgaaaa aacacccaaa
     5401 cgcagcatca aacgttttct gtataaataa accggttgca ttcccggcca ggcggatttt
     5461 tatttactca aaaaggctgc tgcctgtgaa aacaccccat tgaaacacaa attgaccagg
     5521 tcgttttgtt ctttttgccc ctttataatg gggaagggtt gttcgtggct gtattcgtag
     5581 gggaagtcaa tcaggctact gtgtctttga gcgcattctg tacccagagc ttctactatg
     5641 ccgttgtagg ggattacgct gtcttttttc aaggccatca cctggatttt gttccccatt
     5701 tcattgaaga tacggtagcg ctcttcagca ccgcgctctt tctcgatcat ggtgttgaaa
     5761 gcaatcatgg aggcttccga agtttggtca atccggttgt cgctttcgaa gcgatagaga
     5821 taataatcgc ataatttatc gaaggtgagc ttgtccataa taaaacgcga tgttccgttc
     5881 atttgcttga aaatagaacc tccgcaaaac aaaaagagtc cggagtcttc caataaaccc
     5941 tctttatcgc tcagtagtgc tatttgaact aaaaaagcac cgatggaata ggcaaaaaaa
     6001 tccactccgg ttcctgaagt aaacaaagga tggcgccctt ctttgatttg tctcaccagg
     6061 ccggtcaaat cgctgagact ttgtttgccg gaacggtaaa aacgcttagg gtcttcgctt
     6121 aggcgttcac tcaaaatcgt atttgcaatg ctttgagatt gttccttgcc caggatttgc
     6181 cttcgttttt ccaaaatagg agagatcaat cggggattga gccaactcag cggtgaccgg
     6241 ttgatatgaa aggcaatggg cagcaagaca actgctttcc ctgtgtgcat acatagatat
     6301 tctgcccagc acaggtattt gttccaactg cgttcattca gtccgtgcat cagaaatatg
     6361 gctcccgggc acttatcctg actttttagt ttgaatatgg gatagacaaa ttcgcgattt
     6421 tccagaatat cggagtcgga tcctcgggcc ttttcccctt tatcttcgtc aagagatgta
     6481 taagggaaat gacgaatttc aaccggtgaa ttctccagtt tggtatctat tcccaattta
     6541 aattgtcttt gaagttcctc ttgtctttgc gctaaattca tagcctgtcg ttttaagaac
     6601 gaaacaaaaa aacaaaggtc tttctacttg agcaaaaaaa agggacttag ggggattttg
     6661 ggggattttt gtccaaatac agggtgaaaa ccctctatgt gggacgaaat tactttacct
     6721 cttgcgggta aaatgtaaaa tttctatctt tgtacttatt ctcaatagag ggaagcccct
     6781 gatttatgga cagaaagatt ccggattaca ttaacgaaaa aggcaatgtg gtgcgcttgg
     6841 tggtacttac tgctgtcttt gctctactgt ttataaatat ttatgagcct ttcggctcaa
     6901 aaaattggca ggggggagtt tcggatctta cttactttct tttttccaac ctggtaattt
     6961 tggtgggcat gtgtgtggtg gccataagcc gtatcatcat gtaccattac aacaaaaatc
     7021 acgaaatctc cgtccttttg tatctggcat gggtggtgat ggaagtcatt gccatgtcct
     7081 tgttttacac acttttccaa ttttttgcac tcaacgatac gcgtcccttc ctcgaaattt
     7141 ggaaaaatgc caccatcaat accagtttga ttttgttgtt gccttatgcc attctatggt
     7201 tgtatttttc atggcgtgat aacgagaaaa tgctgaaacg tatccgtgaa caggaaagcc
     7261 tgctggcgga gaaacaaaaa agcgggttga tttcttttta cgatgacaag ggagttttac
     7321 gtttatccgt cgatatcaac agcctctatt acatagaatc ggccgacaat tacgtcaaga
     7381 ttcattattt aaacaaaggc aagatgcagc actttatgtt gaggagctcg ctcaaaacca
     7441 tcgatgattt gtttggagac aaatacctgg tgcgttgcca tcgttcgtat atcgttaatt
     7501 ttgacaagat aaaaatactc cgcaaaagtg aagaaggcct cagtctcgag ttggacagcg
     7561 acctggtgcc ggatataccg gtatcaaaaa cttttagcaa caaattaatg agcaggttca
     7621 ccgatctcca gaaaggctcc taatcgcctt ttaacaatac acccttccct ttttagctca
     7681 tttttgggaa agctacactg agcctatttg cttttaacaa tacgcccttc ccctttttgc
     7741 cgtccgtttt aaataccagg ggattttcga atcttacatg ttttaggaac tggttttcga
     7801 acaaggccgg ttgattgtgt atgtattcgt aatcaacgta attataagcc atatcgctgt
     7861 tgggcaggct gtagtaatgg actccaaacg aagtgaggtt ttgaaaaaag tgcgtgccct
     7921 gactgggatc gatctgatat tttcttagac ctgtttcaac tatcagccgg gcattgctta
     7981 tttgagacca gcgaacgggg atgcccatcc aggaatcgct cgatccccaa cgccccggtc
     8041 cgattaaaat gtaattacgt ccttcgtcgc cgagctgctt atttattttc tcaatttcgt
     8101 gtacgcaaag ttgattatta gaggagctaa acagttcggg tttgataaag atcaggtcgt
     8161 aaatatcgtc tactatgcca tggcccagtg cagaaatggt ttttatcagc aattgttctt
     8221 cggggatctc gtccagatct tcgtccaaca tttctttggt atctacaatg gggcgaatct
     8281 gcaaaagata gaagtatctt tgttccgggc tgtcgtcttc gtcgcgtggg tgaaagtcga
     8341 ccgcaaattc aatttcgatg ggtcgtccca tttcctgttc tcccaatttc aaaatcttgg
     8401 ataaaatgtc tgccagtggg aaaacatcgt tttgaagaat atgagcaaag gagattattt
     8461 ttcggcctcc ttcccataat ccgtctctta ttacctgatc gagtgggtcg aaagtggagg
     8521 ctagaaattt aaggttgccg tctttttcgg cttcacttat aggtagctcc agcaagtttt
     8581 gcccgtcgtc aactttgagc cggaaggatt tgtttttgag gtccagagcc agaaaatgcg
     8641 tctgggtttc gctcagggca taattcagcg agctcatttg caacagatgc ttgggatgct
     8701 tgggcgagaa tcttagagtt gtgccgccat cgacaatgta tttacccaga cccagagcca
     8761 cattggcaat tccgtcgccg gcctcttctt cgcctatggg ataataattc agcgaccggg
     8821 caactcccga gaaggtcgga taaaaccgct catcgaacgc agtaccgcag acttcttcga
     8881 gaataatcgc cattttttcc tgatcaatca ggttggaagt ggcttgcata taagccttgc
     8941 tttgttgaaa aaacgctgaa gcgtaaactg ctttaatggc ggactttagc atgatcagca
     9001 tgtcttctgc gtgctcgttg taggggatca tgtaggtgga atagattccg gcaaagggct
     9061 gataatgagc gtcttcgagc aagctggaag agcggatggc gatgggtttt tggacaacat
     9121 ccacaaaagc cgtcaggttt tcgagcagac ggggcggcag ggaagctttg agaaaatttt
     9181 ctaaaataat ttcgtccgtc tgatccgaca tggcaagctc gtgcaaatgg ttattatcca
     9241 tgaattcatc gaaaatatcc gtgcacaaaa ccaaggtttt gggaatgcct atatgtacat
     9301 ttcggtaagt attcagttcc ggatggcttt tgagcatgac atccagaaaa gccaggcctc
     9361 tgcctttgcc tcccagggag ccttcaccga tgcgggcaaa attggaatac ttgtcgaagc
     9421 gtcctttctg aaattctgcc acgatgcctt gattttttac cctgcgatag agaacaatgg
     9481 cgtcaaaaag cattttccgc accggagcag tgtcttcata atcctggata agaatacctt
     9541 tcataaagcg ggcgataggg aacatagccc tggagaacaa ccagcgggag gcgtgatcgc
     9601 gttcaaaatg ataacgtaaa gagtcgtcgg gaatatcgaa aatggccgac tgcagctcct
     9661 gtatgttttg tatacgagcc acttcttcgc cggtttgcgg atttttgaac acaaaatcgc
     9721 cgaaaccaaa ggtatctgcc agcaagtgtc tgagttcttt agaataggtt ttggagcttt
     9781 tttgaataaa acccatttca ttttgctctg cataagtacg gttggaagct tccgaagaat
     9841 taatgatgac gggaagcgcc ttgtcgtatt tcctgaattc attgcacaaa aggaaaccgg
     9901 cgttttgggt ttttttgcct tctttcataa aactgacatc cgagattata cccagcatat
     9961 aatccttgta tttatcgtgt atttccatgg cctcctcgta gctgcgggcc agcagaattt
    10021 tgggacgccc cctcattctg agggtgcgtt ggtgagcatt cagggcttcg gtggcaaaat
    10081 ttcttgattg ctccagcacg tatttataaa gattgggcaa aatggaagaa taggagcgga
    10141 tggaatcttc gaccagcatt attacttgca ctcctacgga ggcaacgtct tcttccacgt
    10201 tcatcttatc ttccataagt ttgataatgg ccagcagcaa atcggaattg cctaaccaac
    10261 tgaatacata atcgatggca ctggtatcgg cattggccag gtattgcgaa acttcgcgtg
    10321 aaaaaggagt cagtactacg atagggatag ccggccattt atgtttaatt tgcttggcaa
    10381 tttcaaaatg ttcgttgctc ttgtcgctgg gcatataaat gataagctcg taattgtttt
    10441 gcccgagttg ttccagagct tcctttttgt tagacacctg ggtaaaccgc ggcggataac
    10501 gcagattaag tgaaatgtat tcgttgaaga tttgttcgtc gaccctgctg tcgtcttcga
    10561 gcataaagaa atcgtactta cttgaaatca gcaaaatgtt gaaaatacgt tttaccatca
    10621 gattggcaaa agaggtatct ttaaaatcca attttttgag gcggtcgtct gcggtcatgg
    10681 tcctaatttg aaatggaaca caaatgtaag caaaaacagc gtgaatcaga gacttgagct
    10741 gtcaaattaa atttgttccg cattggccgg acgatttcct tatatttgca aaaaacccgt
    10801 tcatgcaccc gtcggaatac attaaagaga gtatggaata ccgttttatc cgggaacata
    10861 tcaaagagga tgttcgcaaa ctggctttgc aaacccacgg catgaaggat atgaattatc
    10921 ccttcgcgat taggcagata tccggatatc agcatatcgt gaataaaatt ccggactggt
    10981 atgcctgccc cgatattctc tttcctccaa ctttgtcgct ggagcaatgt tcttcgcagc
    11041 aaacggcttg ctacaaaaga gacctgatga aaacgataat tcggaagcaa aagcaagaac
    11101 agacgataag cggagccgat ttaaccggag gttttggggt agatgcctat tacctgtcgg
    11161 aagtattcga tcggttcttt tacgtggaga aacaacctga attatgccgg atagcaagtc
    11221 ataatttcaa ggctttaaac cgcccgggga tacaagtgtt cgaaaatgaa tcggaagaat
    11281 ttttacaaca aagcggcaag ctggattttg tttacctgga tccctcaagg cgaaaccggg
    11341 atggaaacaa agtaacagct cttcgggatt gtagtcccga cccggcaaca ttgtacgagg
    11401 atttaattaa gaaagccggc ctggtgttga ttaaactttc gcccatgatc gatttgtcgc
    11461 ttgcgctgaa agctttgccc catacccgac agattcatgt ggttgcgctt gacaacgatt
    11521 gcaaagaagt cttgtttctt cttgacgcaa agtatcaaac ggcagaaccc gagatcactg
    11581 ccgtcaatct gaagaataac gccgctgatc aggttttaaa attcagaaga agcgatgagg
    11641 caaatgcaag ctgcgtttac acggcgcaag taggggatta tttgtacgag cccaacgttt
    11701 ccctgttaaa agccggagcg tttatcatgc ccgcccaagc ctatgggctg gaaaaattgc
    11761 ataaaaacac tcatttatac acgtcaaaca aattcaaatc cgattttccg gggaaaatat
    11821 tcgaagtcaa aacaatatcg tcagctaaaa ccaaagattt tcacaaaaaa ttgcccggtc
    11881 tgcaacaggc taatttggtc gtccgcaatg ttccgatgag tgtggaggcc ttgcgaaaaa
    11941 agctcaacct caaagagggg ggagaggatt tcgtttttgc ctgcacttta gccgatgaaa
    12001 gcaaagtatt tctttacggc aaacgattgt tgtaactttg aggattctct gcaaaccggc
    12061 tcatgccgat ttgcagagag cgactaataa catctatgat aaaactaaat aatttaaaac
    12121 tatgatcgaa aacgaaaaaa ctaccaccat tcaggctgcc cttgaggtgg cccggcgcat
    12181 gatgaccgca gcacgtaccg ctcccaaagc cagaggaata gaccgcttgg agctctgttg
    12241 tgtcagcggt gaagaattag aaatcctggc taaaaaaatg gaagagattg gattgaaaaa
    12301 ccggcggcct tcttttgcca gagacgccca aaacataagg cagtcacaag ctgtagtact
    12361 gatcggcagc cgtcagggag aacaagagtt ggattgcgca tattgcggtt ttcccaactg
    12421 tgccgaaaaa cgtaaacatc cggcatttcc ctgtgtcttt ccggttaccg atcttggaat
    12481 tgccctgggt tcggcttgcg ctgtagccgc agacagccgg gtggacagtc gcgtaatgta
    12541 taccgccggt ctggccgccc tggagctggg ttggctgaaa gattgtttct atgctttgtc
    12601 cattccgctt tccatttcgg gtaaaagccc ctacttcgac agaaaataag cgggtttcct
    12661 cttgtctcgt taaccctttg aggagacgat cgcgtcagca gttgaccaca tccgctgcgt
    12721 ttagttttga ttcattgatt ctataagtta tccgatcaag actgcctgga tagctgttgc
    12781 tgtgtttact cttgactggt tggctcctct ttgagcgcct ggaggtcgta cagttttttg
    12841 aagtgaccct tctttctgag caaggctttg tgggtgcctc tttcgacaat acggccttcg
    12901 tgcagcacgc agatcagatc ggcattccgg atagtcgata gccggtgtgc aatcacaatg
    12961 gttgtgcggt ttttcatcag gttttccaga gcctcctgaa ccaatttttc ggattccgta
    13021 tccaaggccg aagtggcttc gtccagtatc agaataggcg gatttttcag aatagcccgg
    13081 gctatgctga tgcgttgacg ttgaccgccc gataatttac tgccgcggtc tccgatattg
    13141 gtgtcgtaac cctgttcggt ggctatgata aaatcgtggg cattggcgat tttagcagct
    13201 tgtaccactt gttcgatgct ggcgtttttt accccaaaag caatgttgtt gtaaaagctg
    13261 tcgttaaaca gaatcgcttc ctgattgaca ttgcccatta gagctctgag gtctttgact
    13321 cttacttgtc ggatatcaat tccgtcgatc agaatctagc cggagttcac atcgtagaat
    13381 cgaggaagca aatcaaccag tgtcgacttt cctgaacccg attgcccgac taaagccacg
    13441 gtctgtcctt tggatatttt gagatcgatg ttttccaaca cgggttcggt ctggtattta
    13501 aagctgacgt ttttgtattc gatggcctcg gtaaatcctt ttatatcaac aggatgttcg
    13561 ggatcggcaa tatgattttc cgtcaagagt atccggtcga ttctttccat ggaggccaga
    13621 cccttctgta cattgtacca ggcttgcgaa aaggctttgg ccggattgat gatgctataa
    13681 aaaataataa ggtaatagat gaataccggt gctgtaatgg tatgagacga ttcggataaa
    13741 atcagcgaac caccaaacca tagtacgatg gcaataatgg ttgtacccag aaattcgctc
    13801 atggggtgag ctagttgctg ccggcggata atccttttgg ttgtgcgttt caaggtctcg
    13861 ttttgccggt tgaaattggc ctttattttg ttctctgcat taaaagcctt gattattctg
    13921 aggccggtca gtgtttcttc tatttgacta agtagttctc cccattgtgc ttggctttcg
    13981 cgcgagggtt ttttcaattg tttgcttacc ctacccatca ggaagcccgc tacgggtaac
    14041 aatacgagca caaaaagcgt aagctcccaa ctgataatca acagggtggt aaaataaatg
    14101 atgatcataa tgaagttctt aaaggtcata tccagtgaac tcatcaccga attctccacc
    14161 tctgttacat catttgacat gcgcgaaatt atatcgcctt ttttttcttc ggagaaaaac
    14221 cccaggggta aattcagtac tttttcgaag atcgtattgc gtaagtccct caagacggaa
    14281 gttcgtatgg taatcatgtt gagcgaggcc atatacgcaa aacctgtctt caggaaagtc
    14341 atggctatca gaaaggctcc caatatcaat aaggtagcac ctcccccgct tttttcgatg
    14401 gtggtggaaa caaaaaagta gaagttatcc ttcagtgctc cggcaatatc ggctgcgggt
    14461 aggctccaga cgttttcgtg gtaggtgtag gtctgttcgt tgatccggaa taagatttcc
    14521 agtatgggaa taaccagggc aaaggaaaac aggctgaaca aagtcgaaaa gaaattgttc
    14581 agcacattga ggaacacact ttttttgtaa ggcgggacca aacgaaacag cagtcgtata
    14641 aagtctttca ttatggggca gggtagagat aaacggccga aagatagtgc atataatctg
    14701 gaaaaaaaag cctcgaaaaa tccttcgagg ctttaggaga gtttagctct atctatgctt
    14761 tcgacaccgg ctgcggttct tggacgccca cgagcatacg gcctgtttaa agccctccat
    14821 gctttcagcc accgtgggtg cttttggctt tagttgctga ttttcttgtc gttttatttt
    14881 gatcattcga ataatatttc atttgttttc ggtcttcgtc ttcccttttc aaaaaagaat
    14941 ttttcttctc caaatgcggg ttctaattgg tcaaaccaag ttgcttccgg gtcgtacttt
    15001 gcaaaaaatg gaatatccac ccattttgca tttcgccctt gtagaaatcg tagcacatac
    15061 actttttcac cttttacttc ttgaactcca agcacttgaa ttttgccggg ctcacagctc
    15121 atgcttggac ctcttaccgt tctacaaagt ccgcttacct gtctgtatgc ctttcggaag
    15181 atattccagc atttatccaa aggaagctca aagtatcgct ttgaacccgt atcgcgtgct
    15241 acaaacatat aatagggaat acatcccaaa tctacctgct ttctccacat ttcggcccat
    15301 atctcgggtt tatcgttgat gttctttagc aagggggatt gtgtgcgaat ttgtgctccc
    15361 gtagcccgaa tttgtttgat tgccttctta actgcgtcgg ttttcagttc cgccggatga
//
LOCUS       DAOSGL010000014        15295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_3174_length_15295_cov_4.137205, whole genome shotgun sequence.
ACCESSION   DAOSGL010000014 DAOSGL010000000
VERSION     DAOSGL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3174_length_15295_cov_4.137205"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..434)
                     /gene="rpsM"
                     /locus_tag="PKY68_00960"
     CDS             complement(54..434)
                     /gene="rpsM"
                     /locus_tag="PKY68_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOH23639.1"
                     /translation="MAIRIVGVDLPQNKRGEIALTYIFGIGRSSAQKILAEAGVDKDI
                     KVKDWTDDQAGAIREIIGHSYKVEGDLRSEVQLNIKRLMDIGCYRGIRHRIGLPVRGQ
                     STKNNARTRKGKKKTVANKKKAAK"
     gene            complement(476..592)
                     /gene="ykgO"
                     /locus_tag="PKY68_00965"
     CDS             complement(476..592)
                     /gene="ykgO"
                     /locus_tag="PKY68_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOH23640.1"
                     /translation="MRVRASVKKRSADCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(605..823)
                     /gene="infA"
                     /locus_tag="PKY68_00970"
     CDS             complement(605..823)
                     /gene="infA"
                     /locus_tag="PKY68_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOH23641.1"
                     /translation="MAKQAAIEQDGTIVEALSNAMFRVELENGHEITAHISGKMRMHY
                     IRILPGDKVRVEMSPYDLSKGRISFRYK"
     gene            complement(840..1625)
                     /gene="map"
                     /locus_tag="PKY68_00975"
     CDS             complement(840..1625)
                     /gene="map"
                     /locus_tag="PKY68_00975"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOH23642.1"
                     /translation="MIYLKTEEEIDYLRESSQIVAKALAEVAKHIQPGVTTKYLDQVG
                     EEYIRSCGAVPSFLHYNGYPASLCISVNEEVVHGIPSVQRVLHEGDIVSVDCGAYKNG
                     FHGDSAYTFCVGEVNEELRALMRTTKEALYLGIEQAVDGSRLGNIGHAIQQHCQARGY
                     TVVREMVGHGIGRNLHEGPEVSNVGKRGAGPLLRTGMVIAIEPMINMGRRNIVIENDG
                     WTVRTADRKPSAHFEHTVVVREGKAEILSSFDYIKEVIGERFI"
     gene            complement(1635..2963)
                     /gene="secY"
                     /locus_tag="PKY68_00980"
     CDS             complement(1635..2963)
                     /gene="secY"
                     /locus_tag="PKY68_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOH23643.1"
                     /translation="MKLIETLKNIWKIEELKSRLLTTLLLVVVFRFGSFVVLPGIDPQ
                     MLTGLHAQTREGLMSLLDMFTGGAFSNASVFGLGIMPYISASIVIQLLSIAVPYFQKL
                     QREGESGRRKMNQYTRYLTVAILLLQGPAFLVNLSANGVPLPSGLWFRMSSVILLASG
                     SMFTLWLGERITDKGIGNGVSMIILIGIIARLPQSLFQEFVSRLSANSGGLVMFLGEL
                     LLLLAVTVFAILLVQGTRKIPVQYAKRIVGNKQYGGARQYIPLKVNAAGVMPIIFAQA
                     IMFIPATVIQFSKAGATSGFAIAFSDITGFWYNFIYAILIILFTWFYTAITINPVQMA
                     EDMKRNGGFIPGVKPGKRTADYLDEIMSRITFPGSLFLAFIAIMPAFAKFFGVSSGFS
                     QFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLLKSGRIKGRTGSIAAY"
     gene            complement(2979..3476)
                     /gene="rplO"
                     /locus_tag="PKY68_00985"
     CDS             complement(2979..3476)
                     /gene="rplO"
                     /locus_tag="PKY68_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOH23644.1"
                     /translation="MNLNNLKPAPGSVKTRKRIGRGPGSGLGGTSTRGHKGAKSRSGY
                     SKRRGFEGGQMPLQRRLPKFGFTNINRVEYKAINLDILQTLAESKSYKVINRDVLVQA
                     GFISATQKVKILAKGSLSANLHVVADAFSAAAVKAIEAAGGKAQLVSEFTAPPQKEEN
                     KAIEE"
     gene            complement(3487..3663)
                     /gene="rpmD"
                     /locus_tag="PKY68_00990"
     CDS             complement(3487..3663)
                     /gene="rpmD"
                     /locus_tag="PKY68_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOH23645.1"
                     /translation="MSVIKIKQVKSRIGAPKDQKRTLDALGLKKLNSVVEHEENAAIL
                     GMVNKVKHLVKIVE"
     gene            complement(3677..4195)
                     /gene="rpsE"
                     /locus_tag="PKY68_00995"
     CDS             complement(3677..4195)
                     /gene="rpsE"
                     /locus_tag="PKY68_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOH23646.1"
                     /translation="MAGQLNRVKTTNDLELKDKLVAINRVTKVTKGGRAFSFSAVVVV
                     GNEDGIVGYGLGKAGEVTAAISKGAEAAKKNLIKIPVHKGTIPHEQSAKYSGAKVLIK
                     PATPGTGVNAGSAMRAVLESAGVTDVVAKSLGSSNPHNMVKATIKALSELRDAYTVSQ
                     NRGISVERVFKG"
     gene            complement(4200..4544)
                     /gene="rplR"
                     /locus_tag="PKY68_01000"
     CDS             complement(4200..4544)
                     /gene="rplR"
                     /locus_tag="PKY68_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOH23647.1"
                     /translation="MTTKELRRIKIKQRVRSKSFGTAASPRMTVFRSNKQIYVQFIDD
                     REGKTLASASSLKLETKTGKREQAAKVGEMAAKNAIEAGITTVVFDRNGYLYHGRIKE
                     LADAARKGGLKF"
     gene            complement(4566..5123)
                     /gene="rplF"
                     /locus_tag="PKY68_01005"
     CDS             complement(4566..5123)
                     /gene="rplF"
                     /locus_tag="PKY68_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOH23648.1"
                     /translation="MSRIGKLPIVIPSKVSVEVDKNNVVTVKGPKGELQQALTGGITA
                     EVKDNHVYVSRPTDGKEHRSLHGLYRALIHNMVVGVSEGFRSVQELVGVGYRASNDGQ
                     LLKLALGYTHEIYLLLPDEVKVETKSERNKNPLIMLESCDKQLLGQICAKIRSFRKPE
                     PYKGKGIRFQGEEVRRKAGKSAVKK"
     gene            complement(5142..5537)
                     /gene="rpsH"
                     /locus_tag="PKY68_01010"
     CDS             complement(5142..5537)
                     /gene="rpsH"
                     /locus_tag="PKY68_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOH23649.1"
                     /translation="MTDPIADYLTRLRNAISANHRVVEVPASNLKKEITKILLQKGYI
                     LNYKFVEDGPQGSIKIALKYDPLSKQNAIKKLIRVSTPGLRKYVGHREIPRVLNGLGI
                     AVISTSRGVMTDKEAKELKLGGEVLCYVY"
     gene            complement(5598..5867)
                     /gene="rpsN"
                     /locus_tag="PKY68_01015"
     CDS             complement(5598..5867)
                     /gene="rpsN"
                     /locus_tag="PKY68_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOH23650.1"
                     /translation="MSKESMKAREVNRAKLIAKYAEKRARLKAEGNYEALQDLPKNSC
                     PVRIHNRCKITGRPKGYMRQFGISRIQFREMASQGLIPGVRKASW"
     gene            complement(5882..6433)
                     /gene="rplE"
                     /locus_tag="PKY68_01020"
     CDS             complement(5882..6433)
                     /gene="rplE"
                     /locus_tag="PKY68_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOH23651.1"
                     /translation="MNTANLKKEYQERIIPALTKEFGYTSVMQVPKLEKIVINQGLGK
                     AVADRKIIETAIAELTAITGQKAVVTTSKKDISNFKLRKKMPIGAMVTLRRDKMYEFL
                     ERLVRVALPRIRDFKGIESKLDGHGNYTLGVQEQIIFPEINIDNITHLAGMNITMVTS
                     AKTDEEGYALLREFGLPFKNINN"
     gene            complement(6433..6753)
                     /gene="rplX"
                     /locus_tag="PKY68_01025"
     CDS             complement(6433..6753)
                     /gene="rplX"
                     /locus_tag="PKY68_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOH23652.1"
                     /translation="MTKLHIKKGDTVYVNAGEDKGKTGRVLEVDVQKRRAIVEGVNFV
                     SRHTKPNAKSPQGGILKKEAPVHISNLNPLDPKSGKPTRIGRVRNEKGKLVRISKKSG
                     EEIK"
     gene            complement(6776..7141)
                     /gene="rplN"
                     /locus_tag="PKY68_01030"
     CDS             complement(6776..7141)
                     /gene="rplN"
                     /locus_tag="PKY68_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOH23653.1"
                     /translation="MIQAESRLNVADNSGAKEVLCIRVLGGTGRRYASVGDIIVVAVK
                     NVIPSSEIKKGVVSRAVIVRTKKEIKRADGSYIRFDDNACVMLTNAGELRGSRIFGPV
                     ARELRNTNMKIVSLAPEVL"
     gene            complement(7144..7401)
                     /gene="rpsQ"
                     /locus_tag="PKY68_01035"
     CDS             complement(7144..7401)
                     /gene="rpsQ"
                     /locus_tag="PKY68_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOH23654.1"
                     /translation="METRNLRKERYGVVIGNKADKSITVVSKFKEKHPIYRKFVSKTK
                     KYYAHDEKNECNIGDTVRIMETRPLSKTKRWRLVEIIERAK"
     gene            complement(7413..7610)
                     /gene="rpmC"
                     /locus_tag="PKY68_01040"
     CDS             complement(7413..7610)
                     /gene="rpmC"
                     /locus_tag="PKY68_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOH23655.1"
                     /translation="MKAKEVRELTTKEIMERVDAEVDRLNTLTLQHSISPLDNPVQIK
                     ESRRLIARLRTILVERNKENN"
     gene            complement(7616..8047)
                     /gene="rplP"
                     /locus_tag="PKY68_01045"
     CDS             complement(7616..8047)
                     /gene="rplP"
                     /locus_tag="PKY68_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOH23656.1"
                     /translation="MLQPKRTKFRRQQKGRMKGNAQRGNQLAFGSFGIKTLENKWITG
                     QQIESARVAVTRYMQRQGQIWIRIFPDKPFTKKPAEVRMGKGKGAPEGFVAPVTPGRI
                     LIEVEGVPYDVAKEALRLAAQKLPVTTKFVVRRDYDQNQNV"
     gene            complement(8066..8815)
                     /gene="rpsC"
                     /locus_tag="PKY68_01050"
     CDS             complement(8066..8815)
                     /gene="rpsC"
                     /locus_tag="PKY68_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOH23657.1"
                     /translation="MGQKVNPISNRLGIIRGWDSNWYGGDSYGDSLLEDSKIRKYLNV
                     RLAKASVSRIVIERTLKLVTITVCTARPGIIIGKGGQEVDKLKEELKKITDKDVQINI
                     FEIKRPEMDAVIVANNIARQIEGKMAYRRAVKMAIASTMRMGAEGIKVQVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYALAEALTKVGLIGIKVWICRGEIYGKVDLAPQFGT
                     TQGSGRGERSFAGERREDRKGGFRKKRNSNR"
     gene            complement(8787..9230)
                     /gene="rplV"
                     /locus_tag="PKY68_01055"
     CDS             complement(8787..9230)
                     /gene="rplV"
                     /locus_tag="PKY68_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOH23658.1"
                     /translation="MGARKRIAADARKEALKTMYFAKLNGVPTSPRKMRLVADMIRGK
                     EVFLALGILKFSSKEAAGRMEKLLRSAISNWEQKNERKAEAGELYVSEVKVDSATMLK
                     RLRPAPQGRGYRIRKRSNHVSLSINTISKNEQNIKSDGTESKSDK"
     gene            complement(9246..9515)
                     /gene="rpsS"
                     /locus_tag="PKY68_01060"
     CDS             complement(9246..9515)
                     /gene="rpsS"
                     /locus_tag="PKY68_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOH23659.1"
                     /translation="MSRSLKKGPYINVKLENKVLAMNESGKKSVIKTWARASMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFSPTRTFRGHSGNRKK"
     gene            complement(9537..10361)
                     /gene="rplB"
                     /locus_tag="PKY68_01065"
     CDS             complement(9537..10361)
                     /gene="rplB"
                     /locus_tag="PKY68_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOH23660.1"
                     /translation="MAVRKLKPTTPGQRHKIIGAFDTITASAPEKSLVFGLTKSGGRN
                     NAGKMTMRYIGGGHKRRLRVIDFKRNKDGVPATVKTIEYDPNRSARIALLYYADGEKR
                     YIIAPNGLEVGMTIMSGDNAAPEIGNALALKNIPVGTVIHNIELRPGQGAKMVRSAGA
                     FAQLVSKEDNYVGIRMPSGEIRRVLDSCKATVGSVGNSDHSLERSGKAGRSRWLGRRP
                     RTRGVAMNPVDHPMGGGEGRASGGHPRSRKGLLAKGLRTRYPKKHSSKYIVERRKK"
     gene            complement(10368..10658)
                     /gene="rplW"
                     /locus_tag="PKY68_01070"
     CDS             complement(10368..10658)
                     /gene="rplW"
                     /locus_tag="PKY68_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOH23661.1"
                     /translation="MGIIIKPIVTEKMTMITEKLNRYGFRVLPEANKIEIAKAVEKMY
                     NVTVVSVNTSNYRGKNTSRYTRGGVIKGKTAAYKKAIVTLKEGDVIDFYSNL"
     gene            complement(10670..11299)
                     /gene="rplD"
                     /locus_tag="PKY68_01075"
     CDS             complement(10670..11299)
                     /gene="rplD"
                     /locus_tag="PKY68_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOH23662.1"
                     /translation="MELNVYNIKGEDTGRKVVLSDSIFAVEPNDHVLYLAVKQHLANK
                     RQGTHKTKGRSEITGSGRKLGRQKGGGSARRGDIKSPIMVGGGRAFGPQPRDYSFKLN
                     KKVKSLARKSALTYKMRDNAIVVVEDFQFEKAKTRDFVSIMQNLHLPEKKVVFVLSEA
                     NKNVYLSARNLPKVQVVMFSELDTYKILDAKTLVLMEGSLAGIEQTFNA"
     gene            complement(11299..11916)
                     /gene="rplC"
                     /locus_tag="PKY68_01080"
     CDS             complement(11299..11916)
                     /gene="rplC"
                     /locus_tag="PKY68_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOH23663.1"
                     /translation="MPGLLGKKIGMTSVFSADGKNIPCTVIEAGPCVVTQVKTVEKDG
                     YRAVQLGFQEKTEKHTNKAEQGHFGKAGVAPQRHLVEFKDFGDEIKCGDVITSELFEA
                     DEFVDVVGTSRGKGFQGVVKRHGFSGVGGASHGQHNRLRAPGSLGASSWPARVFKGMH
                     MAGRTGGERVTIENLQVIKVIPEHHLVLVKGSVPGAKGSIVIIYK"
     gene            complement(11961..12266)
                     /gene="rpsJ"
                     /locus_tag="PKY68_01085"
     CDS             complement(11961..12266)
                     /gene="rpsJ"
                     /locus_tag="PKY68_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOH23664.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKAVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELCSFKRLIDIYNSSSKTVDSLMKLELPSGVEVEIKV"
     gene            complement(12327..14447)
                     /gene="fusA"
                     /locus_tag="PKY68_01090"
     CDS             complement(12327..14447)
                     /gene="fusA"
                     /locus_tag="PKY68_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH23665.1"
                     /translation="MVKADFQYTRNIGIMAHIDAGKTTTSERILFYTGLTHKMGEVHD
                     GAATMDWMEQEQERGITITSAATTTFWKYNNNKYKINLIDTPGHVDFTVEVERSLRVL
                     DGAVAVFCAVGGVEPQSETVWRQADKYNVPRVAYVNKMDRSGADFFSVVSQLRTILKA
                     NPCIIQIPIGAEETFKGVVDLIKMKALLWHDETLGAEYSVEEIPAGLRAEAEEWRDKM
                     LEQVAEYDDRVMQKYFDDPSTIKEEEIHQALRKATLMQALQPVLCGTSFKNKGVQTLL
                     DAVCAYLPSPMDTEAIEGRDPNNPGRVLLRHPDDREPLTALAFKIATDPYVGRLCFVR
                     VYSGVLKSGSYVLNTRNNRKERVSRLFQMHSNKQNPVDVIGAGDIGALVGFKDIRTGD
                     TICDENKPIVLESMDFPDPVIGVAVEPKTQKDLDKLGAGLSKLAEEDPTFTVHTDEQS
                     GQTIISGMGELHLEIIIDRLRREFKVECNQGPPQVSYKEAITREVQLREVYKKQTGGR
                     GKFADIIVKIGPKDADFVGDLQFSSEVKGGNIPTEYIPSIQKGFQTAMRNGVLAGYPV
                     DNLKVTVLDGSFHPVDSDALSFEICAIQAFRNACVKATPVLLEPIMKLEVVTPEENMG
                     DVIADLNRRRGQVGGMESNRSGARIIKAQVPLAETFGYVTALRTITSGRATSSMEFSH
                     YSPMTSIMAKAVLKERAGREDLII"
     gene            complement(14459..14935)
                     /gene="rpsG"
                     /locus_tag="PKY68_01095"
     CDS             complement(14459..14935)
                     /gene="rpsG"
                     /locus_tag="PKY68_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOH23666.1"
                     /translation="MRKSKPKKRVILPDPVFNDTKVTKFVNHLMYDGKKNISYEIFYS
                     ALKTVKAKLPNEEKSALEIWKTALDNITPQVEVKSRRVGGATFQVPTEIRPDRKESIS
                     MKNIILYARKRGGKSMSDKLAAEIVDAFNNQGGAFKRKEDMHRMAEANRAFAHFRF"
     gene            complement(15077..>15295)
                     /gene="rpsL"
                     /locus_tag="PKY68_01100"
     CDS             complement(15077..>15295)
                     /gene="rpsL"
                     /locus_tag="PKY68_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOH23667.1"
                     /translation="LTNQKEVNCYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRG
                     TLDTAGVDGCKQRRSKYGAKNPKETSKK"
BASE COUNT         3721 a         3754 c         3157 g         4663 t
ORIGIN      
        1 ttttagctgc aactgtcttt tttgccattg tttattgatt tgatttaaat ttatcactta
       61 gccgctttct tcttgtttgc taccgtcttt ttcttgcctt tacgtgtacg ggcattgttt
      121 ttggtgctct gtccgcgtac gggtagaccg atacgatgac ggattccccg gtaacaacca
      181 atgtccatta aacgtttgat gttcaattgc acttcggagc gtaaatcacc ttcaacctta
      241 taggaatgac cgataatttc gcgaatcgca ccggcctggt catcagtcca gtctttgact
      301 ttaatgtctt tgtccacacc ggcttccgcc agtattttct gagcactact gcgccctatc
      361 ccgaaaatat aggtcaacgc aatttcaccc cttttgtttt gcggcaagtc tacaccaacg
      421 attcttatag ccatacttaa tctgttatta tattaaaagt ttgaattaca acctactatc
      481 cctgacgttg tttaaactta gggttctttt tgtttattac gtataaacgg ccttttctgc
      541 gtacaatctt gcaatcggca ctacgtttct tgactgatgc tcttactctc atttttataa
      601 tattttattt gtatctaaat gaaatccttc cttttgataa atcgtagggg gacatttcca
      661 ccctgacctt gtctccgggt aaaatacgta tgtagtgcat ccgcatcttt cccgaaatat
      721 gtgccgtaat ttcgtggccg ttttccaatt ctacccggaa cattgcattc gacaatgctt
      781 cgactatggt tccgtcctgt tctattgcag cttgttttgc cataaatgat attttcaatt
      841 taaatgaaac gttctccaat aacttctttg atgtaatcaa acgaagacaa tatttcggct
      901 ttcccctcgc gcacaacaac cgtatgctca aaatgagccg atggtttacg gtctgcagta
      961 cgaactgtcc agccgtcgtt ttcgataact atattacgcc tgcccatatt gatcataggc
     1021 tcaatggcaa taaccatccc tgttcttaag aggggacctg ctcctctttt gcctacgttc
     1081 gacacctccg gcccttcgtg taaattacgc ccgataccgt gtcccaccat ttcgcgaaca
     1141 acggtatagc ctctggcctg gcaatgttgt tggatagcgt gaccgatgtt ccccagcctg
     1201 ctgccgtcaa cagcctgctc tatgcccaaa tatagggctt ctttggttgt tctcatcagt
     1261 gcccgcaatt cctcgtttac ttctcccaca caaaaggtat aggctgaatc accgtgaaat
     1321 ccgtttttgt aggcgccaca atctaccgaa actatatctc cttcgtgcaa aactctttga
     1381 accgagggta ttccgtgcac cacctcttcg ttaacggaaa tacaaagcga agcgggataa
     1441 ccattgtaat ggaggaagga agggactgct ccgcaagaac ggatatactc ctctcctacc
     1501 tgatccaggt acttggtagt aactccgggt tgaatatgtt ttgctacttc ggccaacgcc
     1561 ttggccacta tttgactact ttctctaaga tagtctattt cttcttctgt cttcaaataa
     1621 atcattgccc ggctttaata agcagctata cttccggtgc gtcctttgat gcggccggac
     1681 ttcaacaacc cgtcgtaatg cctcatcaag agatgacttt ctatttgctg caaagtatcc
     1741 agcacaacgc ccaccaaaat cagcaaagag gttccgccaa agaattgcga aaagccggaa
     1801 ctgacgccga aaaacttggc aaaagcgggc ataatagcaa taaaggctaa gaacaaggag
     1861 ccggggaagg taattctgga cataatttcg tccagatagt cggctgttct cttgccgggt
     1921 ttaactccgg ggataaagcc accgttacgc ttcatgtctt ctgccatttg aaccgggtta
     1981 atggttatgg cagtgtagaa ccaggtaaac aatataatga gtatggcgta aataaaatta
     2041 taccaaaagc ctgtgatatc actgaaagca atagcaaaac cgctggtggc accggctttt
     2101 gagaattgaa tcacagtggc gggtataaac ataattgcct gagcaaagat aattggcata
     2161 actccggcag cattcacttt gagtggaata tactgacggg ctcctccgta ttgcttgttc
     2221 cctacgattc ttttggcgta ttgaacgggg atttttctgg ttccctgcac caaaagtatc
     2281 gcaaatacgg taacagcgag caaaagcagc aattcaccga ggaacatgac caatcccccc
     2341 gagttagccg acagacggga aacaaactcc tgaaacagag attgaggcaa acgggcaata
     2401 ataccaatca aaatgatcat ggaaacaccg ttaccaatac ctttgtcggt gatgcgttcg
     2461 cctaaccaaa gggtaaacat actacccgaa gccagcagga ttaccgacga catacggaac
     2521 caaagtccgg aaggaagggg cactccgttg gcagagaggt tgaccaaaaa agccggacct
     2581 tgaagcaaaa gaatagccac cgtcagataa cgggtgtatt gattcatttt gcggcgaccg
     2641 ctttctcctt cgcgttgcaa cttctggaaa tagggaacag ctatactcag caactgtatc
     2701 acaatggatg cggaaatgta aggcatgata cccagaccga atactgaagc gttcgaaaaa
     2761 gcgcccccgg taaacatatc cagcaaggac atcaaacctt cgcgggtctg agcatgtaaa
     2821 ccggttaaca tctgaggatc gatacccgga agcaccacaa aagagccgaa gcggaaaact
     2881 accaccaaca aaagagtggt cagtaaacgg cttttgagct cttctatctt ccagatattc
     2941 tttagggttt ctatcagttt cattcgattg tgtgtttatt attcttcaat cgctttattt
     3001 tcttcttttt gaggaggagc tgtaaactcg ctgaccaatt gtgccttgcc tccggcggcc
     3061 tctatagcct taacagcagc agccgaaaaa gcatcggcaa caacatgcaa gttggcactc
     3121 aaactgcctt tagccagtat cttaaccttt tgcgtggcag aaataaaacc ggcttgcact
     3181 aaaacatctc tgttgattac cttataagat ttgctttcgg ccaaagtttg taaaatatcc
     3241 agattgatgg ctttatactc aacacggttg atgttggtaa aaccaaattt gggcaagcga
     3301 cgttgtaaag gcatttgccc tccttcaaaa ccacgccttt tggaataacc cgagcgcgat
     3361 tttgctcctt tatgacctct ggtcgaagta ccgcccaagc ccgatcccgg accgcgtcca
     3421 atacgtttac gagtttttac cgagcctggt gccggtttga gattatttag attcatgctt
     3481 gtattattat tcaacgattt tcactaagtg ttttacttta ttgaccatgc caaggatagc
     3541 tgcattctcc tcgtgttcta ccacactgtt aagctttttc aatccaagag catctaaagt
     3601 tctcttttga tctttaggtg cgccaatgcg acttttaact tgtttgatct ttataaccga
     3661 catgatgttt tcctccttat cctttaaata ctcgttcaac tgaaataccc ctgttttgcg
     3721 aaaccgtata agcgtcacgc aattcactca gagctttaat agtagcttta accatgttgt
     3781 gaggattaga tgagcccagc gatttggcta ccacatcggt aacgccggca ctttcgagca
     3841 cggcacgcat ggcactaccg gcgttgactc cggtccccgg tgtagcgggc ttgataagga
     3901 ctttggcacc gctgtacttg gcagactgtt cgtgaggaat agtgcctttg tgtacaggta
     3961 ttttgatcaa gtttttcttg gctgcttcag ctcctttgga gatagcggct gttacttcgc
     4021 cggccttacc caggccgtag cccactatgc cgtcctcgtt gcctactacc acaacggcag
     4081 aaaagctaaa ggcacgtcca cccttggtaa ccttggttac ccgattaatg gctaccaatt
     4141 tgtctttcag ctctaaatca ttagttgtct taactctgtt caattgtcct gccatgatgt
     4201 taaaatttaa gaccgccttt acgagcggca tcagccaatt ctttaattct tccgtgatag
     4261 agatagccgt tgcggtcaaa aaccactgta gtgatacctg cttcaatagc attcttggcg
     4321 gccatttcac ccacttttgc agcctgttcc cttttgccgg tttttgtttc gagcttcaaa
     4381 gatgaagcag aggccaaggt tttcccttct ctatcgtcta taaattgcac gtaaatctgc
     4441 ttgttgcttc tgaaaacggt catacggggg cttgcggcgg taccgaagct cttggaacgt
     4501 acccgttgct ttattttgat tcttcttaat tctttagttg tcatatctgg tagtgtttta
     4561 tacgattatt tcttgacagc agacttaccg gcctttctgc gaacctcctc accctggaag
     4621 cggatacctt tgcccttgta gggttcgggt ttacggaaag agcgtatctt ggcacaaatc
     4681 tgtccaagca attgtttatc gcaggattca agcataatca gcggattctt gttacgttcc
     4741 gacttggttt ctaccttcac ctcatccggc aaaagcaagt aaatttcgtg tgtataacca
     4801 agagccaatt taagtaattg gccatcgtta ctggcacgat aacccacacc taccaactcc
     4861 tgtacactgc gaaaaccctc ggacactcct accaccatgt tgtgaataag agcacgatac
     4921 aggccatgca gggaacggtg ttctttaccg tcggtgggac gggaaacata tacgtgatta
     4981 tcttttacct ctgcggtgat gcctccggtc aaagcctgtt gcaattcacc tttaggtcct
     5041 tttacagtaa ccacgttatt cttgtcaacc tctacgctta ccttggaggg aatgactatc
     5101 ggcaattttc caattcttga catattattc tcctcctgcg attaataaac gtaacataat
     5161 acttcaccgc caagttttaa ctccttggct tccttgtcgg tcatcacacc ccgggaggtt
     5221 gaaataacag caatgcccaa accgttcagt acgcggggaa tctcgcgatg gcctacatat
     5281 ttgcggagac cgggagtaga tacacgaatc agttttttaa tggcattttg tttactcaga
     5341 gggtcgtatt ttaaggcaat tttaatacta ccctggggac catcttctac gaacttatag
     5401 ttcagaatat aacccttttg taaaaggatt ttggtgatct cctttttcaa attagacgcg
     5461 ggaacttcga ccactctatg gttagcactg atggcattcc gcaatctcgt taaataatct
     5521 gcaataggat ctgtcataat gttggtttaa attaatccgg actccccgga caatatttaa
     5581 ttatataatt tgaatgttta ccagctggct ttacgaacac cgggtatcaa accttgcgaa
     5641 gccatttcgc ggaattgaat acgcgagatc ccaaactgac gcatgtaacc tttaggtctg
     5701 ccggtaattt tgcaacggtt gtgtatgcgt accgggcagg aatttttagg taaatcctgt
     5761 aatgcttcgt agttaccctc agctttgagt ctggctcttt tttcggcgta cttggcaata
     5821 agttttgctc ggtttacctc gcgggctttc attgattctt ttgacatact gctattttat
     5881 cttaattatt tatgtttttg aaaggcaagc caaattccct gagcaaggca tagccttctt
     5941 cgtcggtttt ggcgctggtt accatggtga tattcatgcc tgccaagtgg gtaatattgt
     6001 caatgttgat ttcaggaaag ataatctgct cctgaactcc caatgtgtaa ttaccatgtc
     6061 cgtccaattt gctttcgatc cccttgaagt ctctgatacg gggcaaagct acgcgaacaa
     6121 ggcgttccaa aaattcgtac atcttatcgc gacgcagagt aaccatcgct ccgatgggca
     6181 ttttcttacg aagcttaaag tttgagatgt cttttttgga cgtggtaaca acagctttct
     6241 gtccggtaat ggcggttaac tcggctatgg cagtttcaat aatctttctg tctgctacag
     6301 ccttacccaa accttggtta ataactattt tttctaattt ggggacctgc atcactgagg
     6361 tgtagccaaa ttcctttgtt aaagcaggaa taatgcgttc ttgatattct ttcttgaggt
     6421 tagctgtatt cattacttaa tttcctcccc tgattttttg gatattctga ctaatttccc
     6481 tttttcgttc ctgacacgac caatgcgagt aggcttaccc gatttaggat ccaagggatt
     6541 taaattggaa atgtgaacgg gggcctcttt tttaagtatg cctccctgag ggctttttgc
     6601 attaggctta gtgtgcctgg atacaaaatt gaccccttcg acgatggctc gtcttttttg
     6661 tacgtccact tcgagcacgc gaccggtttt acctttatct tcgcctgcat tcacatatac
     6721 tgtgtcgcct tttttgatat gtaactttgt cattataact ggtatttcta tacaattaaa
     6781 gtacttcggg agccaaagaa actatcttca tattggtatt acgaagctcc ctggcaaccg
     6841 ggccaaaaat acggcttcca cgcaactcgc cggcattggt caacatcaca caagcgttgt
     6901 cgtcaaaacg gatgtaagaa ccgtcggctc tttttatctc ctttttggta cgaactatta
     6961 cggccctgga tacaacacct ttcttaattt cactggaggg aatcacattc tttaccgcta
     7021 ccacgataat atctcccacc gaggcataac gtctcccggt gcctcccagc acgcggatac
     7081 acaacacctc ttttgcacca ctgttatcgg ccacattgag cctgctttct gcttgtatca
     7141 tgattattta gctctttcga tgatttcaac taatctccat ctcttggttt tactcaaggg
     7201 acgggtttcc ataatcctta cagtatcacc gatgttacat tcgttctttt cgtcgtgggc
     7261 ataatacttt tttgtcttgc tgacgaattt tctgtaaatc ggatgcttct ccttaaactt
     7321 actcacaacg gtgatggatt tatctgcttt gttgccgatg acaacaccgt atctctcttt
     7381 tcttaaattt ctggtttcca tcaggctatg tattagttat tttctttatt gcgttcaact
     7441 aaaatggttc tcagccgtgc aatcaaccta cgactttctt ttatctgaac aggattgtcc
     7501 agaggagaaa tgctgtgctg aagcgtcaag gtgttcaggc gatcgacttc tgcgtcgacg
     7561 cgctccatta tctccttagt ggtcaattct ctgacttctt tagctttcat aggtcttata
     7621 cattttgatt ttgatcataa tcacgtctga cgacaaactt agtcgtgaca ggcaactttt
     7681 gggctgccag acgcaatgct tctttggcaa catcgtaagg tacgccttct acctctataa
     7741 gtatcctgcc cggagttacc ggggcaacaa aaccttcggg agctcctttt ccttttccca
     7801 tacggacttc ggcgggcttc ttggtaaaag gtttatccgg gaaaatccgg atccatatct
     7861 gaccctgacg ttgcatataa cgggttacag cgacacgggc agattcaatc tgctggccgg
     7921 taatccactt gttttccaga gtctttattc caaaagaacc aaaggccaat tgattgccgc
     7981 gttgggcatt ccctttcatg cggccctttt gttgtcttct gaatttcgtt cttttaggct
     8041 gtaacattgt ttcgtgatta taatattatc tattgctgtt tctcttcttg cggaagccac
     8101 cttttcggtc ttcacgacgt tcgccggcaa aagacctttc gcctctgccg ctaccctggg
     8161 tagtaccgaa ctgtggagcc aaatctacct taccgtaaat ttcacctctg cagatccaaa
     8221 cttttatacc tatcagacca actttggtca aagcttctgc cagggcataa tcgatgtcgg
     8281 ctctgaaagt atgcaggggt gtacgtcctt ctttatacat ttcggaacgt gccatttctg
     8341 caccgttcaa gcggcctgac acctgtactt tgatgccttc ggctcccata cgcatggtgg
     8401 aggcaatggc catttttacg gcacggcgat aagccatttt cccttctatc tgtcgtgcaa
     8461 tattattagc cacaataaca gcgtccattt cggggcgttt gatctcaaag atattgattt
     8521 gaacatcctt gtcggtgatc ttttttaatt cttctttcaa tttatccact tcctgaccac
     8581 ccttaccgat aatgataccc ggacgggcag tacaaacggt aatggtaacc aatttgagtg
     8641 tacgttcaat tacaatgcgg gaaacactcg ctttggccag acggacattc agatacttac
     8701 gaatcttgct gtcttccaac aaagaatctc cataactatc accaccatac cagttagaat
     8761 cccaacctct gatgatacct aaacggttac ttatcggatt tactttctgt cccatctgac
     8821 tttatgtttt gttcgttttt acttatcgtg ttaatactca ggcttacatg attggagcgc
     8881 ttgcgtatgc gataccctct accctgggga gcgggacgca gccttttcaa catagtggca
     8941 gagtcaactt taacctcgct gacatacaac tcacctgctt cggccttgcg ctcgtttttc
     9001 tgttcccaat tggagatggc ggaacgaagc aatttttcca tgcggccggc agcttcctta
     9061 gacgaaaact tcagtatgcc cagggccagg aatacttctt tgccgcgtat catatcggca
     9121 accagacgca ttttgcgagg cgacgtagga acaccgttca atttggcaaa atacatcgtc
     9181 ttcaaggctt ctttccttgc gtctgctgct attctttttc ttgcacccat tgtctctatc
     9241 tgtttttatt tttttctgtt tccactgtgg cctctgaacg tacgagtcgg agagaattct
     9301 cccaacttgt gtccgaccat gttttcggtg atatacaccg gtatgaactt atttccattg
     9361 tgaaccgcta tggtatgtcc cacaaaatcc ggggaaatca tagaggctct ggcccaggtc
     9421 ttgatcaccg attttttgcc ggattcattc atggctaaaa ctttgttttc aagttttaca
     9481 ttgatatacg gtcccttttt taatgaacga ctcataatat ttcctacttt atcctattat
     9541 tttttccttc tttctacaat gtacttgctt gaatgtttct taggatatct tgttctaaga
     9601 cccttagcca acaaaccttt gcgcgatctt ggatgcccgc cggaagcgcg gccttcgcca
     9661 ccacccatgg ggtgatcaac cgggttcata gctacaccac gagttcttgg acgacgaccc
     9721 agccatctcg aacggccggc tttaccggag cgctccaggc tgtggtctga attgccgaca
     9781 ctgccaactg tggctttgca agaatctaac accctgcgga tttctcccga tggcatacga
     9841 atacctacgt aattgtcttc tttggaaacc aattgcgcaa aagcaccggc ggaacgcacc
     9901 atcttagcac cttgtccggg acgaagctcg atattgtgaa taacggtccc cacgggaata
     9961 tttttcagtg ccagggcatt tccgatttcc ggagctgcat tatctcccga cataatggtc
    10021 atcccgactt caagtccgtt gggagctata atgtatcttt tctcaccgtc ggcataataa
    10081 agcaatgcta tgcgtgccga acggttggga tcgtactcaa tggttttcac tgttgcagga
    10141 acgccatctt tatttctctt gaaatcgata acacggagtc ttcttttatg accgcctccg
    10201 atatagcgca tagtcatttt ccccgcatta ttgcgtccgc ccgatttagt cagaccgaat
    10261 acaagagatt tttccggtgc actagcagtt atggtatcga acgcaccgat aatcttatgt
    10321 ctttgccctg gtgttgtggg ctttaatttt cgtactgcca tcttatttta taaattgctg
    10381 taaaaatcaa tcacatcacc ttctttcaag gtaactatgg cttttttgta ggccgccgtt
    10441 ttgcctttta ttactccgcc tctggtatag cgactggtgt ttttgcctct gtagttgctg
    10501 gtgttaacac taaccacagt aacattgtac atcttttcga ccgccttggc aatttcaatt
    10561 ttattcgctt ccgggagtac acggaatccg taacgattta acttttccgt aatcatcgtc
    10621 atcttttcgg taacgatagg cttaattata attcccattg tttttctcct tatgcgttaa
    10681 aagtttgttc aattccggcc aacgaacctt ccatcagcac cagtgttttt gcgtcaagta
    10741 ttttataagt atctaattca gaaaacatta caacttgcac tttcggcaaa ttacgagccg
    10801 acaaatatac gtttttattt gcttccgaca atacaaaaac tacctttttt tcaggaagat
    10861 gcaaattttg catgatcgac acgaaatctc tggtcttggc tttctcaaac tggaaatctt
    10921 cgaccaccac aatggcgttg tcgcgcatct tgtaggtcaa ggccgatttt cgagccagcg
    10981 acttcacttt tttgttcaat ttaaaactgt aatcacgggg ctgagggcca aatgctctgc
    11041 ctccaccaac cataatcggc gatttgatat ctccgcggcg tgcactgccg ccacctttct
    11101 gacgaccaag ctttctgccg gaacctgtaa tctcactacg tcccttggtt ttgtgcgttc
    11161 cttgcctttt gttagccaaa tgttgtttaa ccgccagata caatacatga tcgttgggtt
    11221 caacagcaaa aatggaatcg ctcaacacaa cctttcttcc ggtgtcttca ccttttatat
    11281 tatatacatt cagttccatc atttatagat tattacaatt gaacctttag ctcccggaac
    11341 agaaccttta accaaaacaa gatggtgttc gggaataact ttgatcacct gaaggttttc
    11401 gatggtcacc ctttctccgc cggtacgtcc ggccatatgc atacccttga aaacgcgggc
    11461 aggccatgag gatgctccca acgaacccgg ggcacgtaaa cggttgtgct ggccgtggct
    11521 ggctccacct acacccgaga atccatgacg cttgacaaca ccctgaaatc ctttacctct
    11581 ggatgttccg acaacgtcaa caaattcatc ggcttcaaac aactcagagg tgatcacgtc
    11641 accacacttg atttcgtcgc cgaaatcttt aaattccacc agatgtctct gaggtgctac
    11701 cccggctttt ccaaaatggc cttgctctgc cttgttggta tgtttttcgg ttttttcctg
    11761 aaaacccaac tgtacggccc gataaccatc tttttctacg gttttaacct gagtaacaac
    11821 acaaggaccg gcttcaatca cagtgcatgg gatgtttttc ccatcggcac tgaaaacgga
    11881 tgtcattccg attttttttc ctaataatcc tggcatttct ctaaattata taattatata
    11941 aatcatgcat ttttgcgcta tcagactttg atttccacct caacgccgct gggtaattcg
    12001 agtttcatca aagaatcaac tgtttttgaa cttgaattgt agatgtcgat cagtctcttg
    12061 aaagaacaca attcaaactg ttcgcgagat tttttgttta cgaaagtaga gcggttcacg
    12121 gtaaatatcc ttttgtgcgt gggcaaagga atggggccgc ttaccacagc tcccgtcgcc
    12181 ttaacggcct ttacaatctt ttcggcggat ttgtccacca ggttgtgatc gtaagatttg
    12241 agtttaattc tgattttctg gctcataaat gttttattaa tggtttattt cggcgatccg
    12301 tagagctatt ttcactgatc tgccgtttat atgattaaat cttccctccc cgcacgttct
    12361 ttgagtacgg ctttggccat aattgaagtc atgggagagt aatgtgaaaa ttccatactg
    12421 ctggttgccc ggcccgacgt aatggtacgc agggcagtta cgtaaccgaa agtctcggcc
    12481 agcggcacct gtgctttaat gattctggct cccgaacggt tggattccat gccgcccacc
    12541 tggccgcgac gtctgttgag gtcggcgatt acatcgccca tattctcttc gggtgtaacc
    12601 acctcgagtt tcataatggg ctccaataac acgggagtag ccttaacaca ggcatttctg
    12661 aaggcctgaa tggcacaaat ctcaaacgag agtgcatcgg agtctaccgg gtggaaggag
    12721 ccatccaata cggtaacctt gagattatct accggataac cggccaaaac cccgttacgc
    12781 atagctgtct ggaaaccctt ctgaatggaa ggtatgtatt cggtgggtat gtttccaccc
    12841 ttgacttcgc tcgagaattg cagatcgcca acaaaatcgg catctttggg gccaatttta
    12901 acaatgatgt cggcaaattt cccccgtccg ccggtttgtt ttttgtaaac ttcacgcaac
    12961 tgcacctccc gggtaatagc ttccttatac gaaacctgag gaggtccttg attgcattcc
    13021 actttgaatt cgcgacgtaa acggtcgata atgatttcca gatgcaattc acccatgcca
    13081 ctaatgatgg tttgtcccga ctgttcatcg gtgtgaacgg taaaagtggg gtcttcttct
    13141 gctaattttg acagaccggc acccagctta tccagatctt tttgagtttt gggctcaacg
    13201 gctacgccga ttacgggatc ggggaaatcc atcgattcca gtacgatggg tttattttcg
    13261 tcacaaatgg tatcgccggt gcgtatatcc ttgaaaccta ccaaagctcc gatatctccg
    13321 gctccgataa catcgacagg attctgttta ttagagtgca tctggaacaa cctcgacacg
    13381 cgttccttac gattgttccg ggtattgagc acataactgc ccgacttgag cacaccggaa
    13441 tagacacgta caaaacacaa acggccaacg tagggatcgg tggcaatttt gaacgccaag
    13501 gcggtgagag gctcccggtc gtcgggatgt ctcaacaaaa ctctaccggg attgttggga
    13561 tccctccctt cgatggcctc ggtatccatg ggactgggca aataagcaca gacagcatcc
    13621 aacagtgttt gcaccccttt gtttttgaaa gaagtgccgc agagtacggg ctgcaaggct
    13681 tgcatcaggg tggccttgcg taaagcctga tgaatttcct cttctttgat ggtggaggga
    13741 tcgtcaaaat atttctgcat cacgcgatcg tcgtactctg ctacttgctc cagcattttg
    13801 tccctccatt cttcggcttc agccctgaga ccggcaggaa tttcttcgac ggaatattcg
    13861 gctcccaaag tttcatcgtg ccacagcaag gccttcattt tgatcaggtc gacgactccc
    13921 ttgaatgttt cttctgcccc aattggaatt tgaataatgc aaggattggc cttgagtatc
    13981 gtgcgcaatt gagaaaccac cgaaaagaaa tcggcacccg aacgatccat cttgttgacg
    14041 taggctacgc ggggcacatt atacttatcg gcctgtctcc agactgtctc cgattggggt
    14101 tctaccccgc cgaccgcaca aaaaacagcc acagcgccgt ccagtaccct gagagaacgc
    14161 tccacctcta cggtgaagtc tacgtgcccg ggggtatcaa tcaggttgat tttatattta
    14221 ttgttgttgt atttccaaaa agtggtagta gcagcggaag taatggtaat accccgttcc
    14281 tgctcctgct ccatccagtc catagtggca gcaccatcgt gcacctcacc cattttgtga
    14341 gtaagcccgg tgtagaaaag aatacgctcc gaagtggtag tctttcccgc atcgatatgg
    14401 gccattatgc ctatatttct ggtgtactgg aagtctgctt ttaccatcgt ctgatcaatt
    14461 aaaacctgaa gtgagcaaaa gcacggttgg cttcggccat tctgtgcata tcttccttgc
    14521 gcttgaatgc tccgccctga ttgttgaaag catcaacaat ttctgcagcc agcttatcgg
    14581 acatggattt acctccacgt ttgcgagcat acaaaataat atttttcata gaaatggatt
    14641 ccttgcgatc cgggcggatc tctgtaggaa cctggaaagt ggcacctccc acgcggcggg
    14701 attttacctc gacttgagga gtaatgttgt cgagggcggt cttccagatt tcgagtgcgg
    14761 atttttcttc gttaggcaat ttagccttta cggttttcaa agcggaatag aaaatttcgt
    14821 acgaaatatt ctttttcccg tcatacatca ggtgattgac aaattttgtc accttggtgt
    14881 cattaaaaac aggatccggg aggataaccc gttttttcgg tttgcttttt ctcattttct
    14941 caaataattg accgttttat tgttttggct gtttgtcttc aacgctccca ccggaacatt
    15001 tactcaaccc ggaaaaacta gtctccctta cttaccaata cgctaaaaac tggtgatgaa
    15061 tgtatgatgc tttaatttat tttttgcttg tttccttagg atttttggct ccgtacttgg
    15121 aacggcgttg tttacaaccg tctacaccgg cagtatcgag tgtgccgcga acaatgtgat
    15181 aacgaacacc gggtaaatcc ttcacacgac ccccgcgaac caacactata gagtgctcct
    15241 gcaggttatg gccttcgccg ggtatatagc agtttacctc tttctggttg gtcag
//
LOCUS       DAOSGL010000015        15202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_3198_length_15202_cov_3.730046, whole genome shotgun sequence.
ACCESSION   DAOSGL010000015 DAOSGL010000000
VERSION     DAOSGL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15202
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3198_length_15202_cov_3.730046"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKY68_01105"
     CDS             complement(<1..534)
                     /locus_tag="PKY68_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOH23668.1"
                     /translation="MAKMNFGGVMENVITREEFPLKKAKETLKNETIAVIGYGVQGPG
                     QSKNLRDNGFNVIVGQRKGGATWDKAVADGWVPGETLFEIEEACRRGTIIQYLLSDAA
                     QIEVWPRIKPHLTAGKALYFSHGFAITYKDRTNIIPPKDIDVILVAPKGSGTSLRTMF
                     LEGRGLNSSYAIFQDATG"
     gene            complement(559..1119)
                     /gene="ilvN"
                     /locus_tag="PKY68_01110"
     CDS             complement(559..1119)
                     /gene="ilvN"
                     /locus_tag="PKY68_01110"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOH23669.1"
                     /translation="MMEKTLYTVTVYSENQVGLLNQVTIVFTRRQVNIESLTVSRSAI
                     PGVHKFTITANTDRETIRKIVLQIEKRIDVLKAYYYTDDQIIFQEIALYKVPTLSLMS
                     SNEIETLVRKYNARILEVTKDYTVIEQAGHKAEIDAMFDALNKNKILQFVRSGRVAIT
                     RSTVERLSNFLEEMEARRKEVFNGNN"
     gene            complement(1121..2824)
                     /gene="ilvB"
                     /locus_tag="PKY68_01115"
     CDS             complement(1121..2824)
                     /gene="ilvB"
                     /locus_tag="PKY68_01115"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOH23670.1"
                     /translation="MEKKKITGAEALLFAFKEEKVDTIFGYPGGAIIPVFDALYTHKE
                     KLALNHILTRHEQGAAHAAQGYARVSGKVGVVIVTSGPAAANIVTGVADAMLDSTPLV
                     VVTGQTSVDALGSDAFQETDVLGITQAITKWSYQIRRAEDISWAVARAFYIAREGRPG
                     PVVLDLSKDAQNQLIEFSYEKCRYIRSYQPEPELNPDKIARAAQLINQAKKPFAVVGQ
                     GVLLGRAEQELFNFLNKADIPAASSLLGLDVMPVDYRLNMGMVGMHGNVAPNKKTNEC
                     DVLIAIGMRFSDRVTGKLSEYAPQAKVIHFDIDPSEINKNVQVEVGVLGNVKKSLPAI
                     TELLDKADHKEWIESFEEHKLCEYREVVSKDLYPNNGILTMAEVVKCLSDNTGENTIV
                     VTDVGQNQITAARYFSAYNTRKIVTSGGLGTMGFGLPAGVGAALGSPESKVFVISGDG
                     GIQMTIQELGTIMEYKIPVKVIILNNNYLGMVRQWQELFYEERYSSTSIQNPDFIALA
                     AAYGIKGRKVEQRKDLAEAVREMIAADSAYVLEVMVEKKGIVYPMVPAGKCITEIMLK
                     S"
     gene            complement(2828..4660)
                     /gene="ilvD"
                     /locus_tag="PKY68_01120"
     CDS             complement(2828..4660)
                     /gene="ilvD"
                     /locus_tag="PKY68_01120"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOH23671.1"
                     /translation="MKHPLRSAVCTEGRRMAGARALWLANGMKKEQLGKPIIAIVNSF
                     TQFVPGHVHLHNIGQEIKAEIEKAGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDLIA
                     DSVEYMVNAHKADAMICISNCDKITPGMLMAALRMNIPSVFVSGGPMEAGKLGDTYLD
                     LIDAMVQAADPKVSDEELAAVEAVACPGCGSCSGMFTANSMNCLNEALGMALPGNGTV
                     LATHVNRKKIFQQAARQIVHNTFAYYRDGDQRVLPRSIASKAAFMNAMALDIAMGGST
                     NTILHLLALAQEAEVDFTMDDIDALSRKIPCLCKVAPSSHYHVQDVNRAGGILSIMDE
                     LDQAALIDNSVYRVDGYTLKEAMDKYSIRSANVLPEALALYKSAPGGKFNNTLASQEA
                     VYKELDTDRSLGCIRDIEHAYTRDGGLAILKGNIAQDGCVVKTAGVDESIWKFSGKAK
                     VFHSQEAACDGILDGRVEAGDVVVIVFEGPKGGPGMQEMLYPTSYIKSRGLGKSCALI
                     TDGRFSGGTSGLSIGHISPEAASGGAVALIRDNDIIDIDIPHRSINLRIGAEELNQRR
                     LEEEKKAAGAFKPKRDRKVSKALKAYAGMVSSADKGAVRIIEED"
     gene            complement(5139..5612)
                     /gene="rlmH"
                     /locus_tag="PKY68_01125"
     CDS             complement(5139..5612)
                     /gene="rlmH"
                     /locus_tag="PKY68_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOH23672.1"
                     /translation="MKVILLLSGKTGEDYARKAIKLFEERLKHYLTFEIVYLPELKRS
                     GKISEQEQKQREAALLEPWIKPENTLVLLDERGREYTSEAFAAYLQKKMNAGLRNLVF
                     VCGGPYGFDESVYKRVPERLSLSKMTFSHQMVRVFFVEQLYRAMTILRGEPYHHS"
     gene            5717..6556
                     /gene="nadC"
                     /locus_tag="PKY68_01130"
     CDS             5717..6556
                     /gene="nadC"
                     /locus_tag="PKY68_01130"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOH23673.1"
                     /translation="MASLEDFIRNAFAEDIGEGDHTSLSCIPASARGKSMLLIKEAGV
                     VAGVRVALEVFRLFDPQLQTTVFIQDGQKVKPGDIVFETAGSVRSLLQTERLMLNIMQ
                     RMSGIATTTRLYADRLQGLHTKVIDTRKTTPGMRLLEKEAVVLGGGSNHRMGLYDMIL
                     LKDNHVDFAGGIAKAIAATRGYLQEKKLDLKIEIEVRSLDELEQVLQCGGVDRIMLDN
                     FDPATTRKAVERIAGRYETESSGGITLDTIRAYAECGVDFISVGALTHSIPSLDMSFK
                     AVL"
     gene            6553..7224
                     /locus_tag="PKY68_01135"
     CDS             6553..7224
                     /locus_tag="PKY68_01135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOH23674.1"
                     /translation="MNYTLIRSRRKTLTLQIKPDGSLLVRCPLRLNLREVERFIQAKQ
                     PWILSKQQLLRENFEKRQAFRLHPGMELRYLGKTYPLRLSENRKSGFDSRGFYLGNEV
                     PEEKIRATVVKIYKELATTHITAKVEHYARLYKLKYTDVRIGSAKTRWGSCSSKNRLI
                     FSWRLIMADELCVDYVVLHELAHTLEHNHSHQFWTKLGRMMPNYPEARRQLRIFGRQL
                     QAEDW"
     gene            7412..9046
                     /gene="pruA"
                     /locus_tag="PKY68_01140"
     CDS             7412..9046
                     /gene="pruA"
                     /locus_tag="PKY68_01140"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOH23675.1"
                     /translation="MSTGFYTIPEIRNEPVKTYAPGTPERAALKKTLAELRSKEADIP
                     MVIGGREIRTGKKVRIFPPHEIKHTLGYYYQGDGSHVQMAIDAALAARDKWAAMSWQH
                     RSAIFLKAADLLAGPYRQKINAATMLGQSKNAFQAEIDAACELADFFRFGVRCVTDIY
                     KMQPESSPGMWNYNEFRPLEGFVFALTPFNFTSIAGNLPAAPAMLGNVAVWKPSKTAI
                     YSAAVIMELFREAGLPDGVINLVFADGPTTSNVVLNSPYFAGIHFTGSTGVFQSIWKT
                     IGENIYKYKSYPRIVGETGGKDFIFADPTADPQALTIALLRGAFEYQGQKCSAASRAY
                     IPAGLWEDIRERLGKELATVKMGPPEDFSNFINAVIDEASFDKLKGFIDRARTDADAE
                     VIFGGNCDKSVGYFIEPTVILAKKPDYITMIEELFGPILTIYVYKDEELDATLDLLDR
                     SSIYGLTGAIFSQDRYNIEKMTKKLVNAAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSVFNFLRWTSIRTIKETFVPPTDYMYPNFLPGD"
     gene            9141..9683
                     /locus_tag="PKY68_01145"
     CDS             9141..9683
                     /locus_tag="PKY68_01145"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOH23676.1"
                     /translation="MNKAIRKIGLVAHDGMKQDLIEWAVWNSERLIGHKFYCTGTTGK
                     LVQEALSRKHPDIDWDITILKSGPLGGDQQMGSRIVEGEVDYCFFFTDSMTLQPHDTD
                     IKALTRLAGVENIVFCCNRSTADHVISSPLFLDPGYKPIKKDYSSYTNRLKDRDLISK
                     AVESSRSQKRRKKRSKPGNE"
     gene            9706..11730
                     /locus_tag="PKY68_01150"
     CDS             9706..11730
                     /locus_tag="PKY68_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23677.1"
                     /translation="MKKYFVVISVVLLMISCKKTADTNLPESTVQQALTACTAQSELP
                     DDLIEKGLRQCAGLWTPADGSPEEFVEFCKIHLCADAQQKSLLFERMADHFETLLGHQ
                     NRVRVELMRPLHETGYQNLPVDQLFGAYDNAAHFEEDMYGSKIAFVVALNFPYFSLDE
                     KTALAEEWTSEEWGYARLGDFFIERIPASVKQEMTLAATRAEDYISNYNIHMGSLVDR
                     GEKLFPDHMVLISHWGLRDELKSNYADKERGLQKQEIIYRVMQHIIEQSIPLQVIDNP
                     NYDWDPINNKLYLEGKEIAVEAEPDTRYEHFLNLFKTYKAQDNYSRQYPSYIARYFEG
                     ELEIAEKEVEALFVELLSSKQAALTGQLIAQRLGRKLRPYDIWYDGFKSRSSIDPAVL
                     DKIVLDKYPSLQAFEQDLPVILVKMGFSKQKAKEICAQIEVNPSVGSGHAWPAAMKND
                     KIHLRARFRENGVDYKAYNIATHEFGHNVEEYISLYDVPNYFLSSVPNNAFTEALAFV
                     FQERDLDLLGLKQQDPLAASLNTLDLFWSSYEIMGVALVDMKVWRWMYENPSTDAVQL
                     KENVIRIAKGVWNDYYAPVFGIEDQNILAVYSHMIEIPLYLPAYPLGHLIHFQLNEHF
                     EGKDMGAEIQRIYALGRLSPQYWMQKAVGSKLSAEPLLKATSLALEQVMQ"
     gene            complement(11792..12265)
                     /locus_tag="PKY68_01155"
     CDS             complement(11792..12265)
                     /locus_tag="PKY68_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH23678.1"
                     /translation="MGAELQFELCRWDNSRQTGAFLSLLNHYMADPMGNYPPLDGAEQ
                     QKLLADLSTHPTVEVLLMSLSGEYIGMTTFFVNYSTFRLKPYIYIHDVVVHKTQRNRG
                     YGQSMIKELVRLAQERDYCKLTLEVRNDNPAAQKVYSGLGFEAGRPEMLFWTKWL"
     gene            12495..13145
                     /locus_tag="PKY68_01160"
     CDS             12495..13145
                     /locus_tag="PKY68_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH23679.1"
                     /translation="MKRNSLFKDNYWLFIPYLLMLAISVFFILFYSKAEIHIWMNQHH
                     SPFADLLFKYASYMGEGWVLTPICLILLFVRFRYAFMAIGSSILAPLFTQFFKRVVWP
                     DSPRPKVFFENLYDLHVVDGVHLHSAHSFPSGHATGAFAVFMVLALIVKKPFWKVFFL
                     ILASLTAYSRVYLSQHFLVDVSVGSLIGSLTALLCYCWMMRYSKGWLDKSFKICVK"
     gene            complement(13097..13711)
                     /gene="yihA"
                     /locus_tag="PKY68_01165"
     CDS             complement(13097..13711)
                     /gene="yihA"
                     /locus_tag="PKY68_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOH23680.1"
                     /translation="MEINSASFVVSNTSVENCPKTSLPEFAFIGRSNVGKSSLINMLL
                     NQKNLAKTSSTPGKTLLINHFLVNEAWYLVDLPGYGYARVSLKQREQLHRMISDYMLK
                     RPNLLCVFVLLDSRHRPQEIDLDFLEFLGENQVPFALVFTKIDKCKKSELNLNIENYR
                     NRLLESWESLPPLFLSSSEKKQGRTALLTYLTQILKDLSSQPLE"
     assembly_gap    13745..13844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13868..>15202)
                     /locus_tag="PKY68_01170"
     CDS             complement(13868..>15202)
                     /locus_tag="PKY68_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOH23681.1"
                     /translation="TFVSVPGMVRDNSFLYMQTVMGFFVGYILIANVLLPVYYRLNSP
                     SIYVYLKERFGKYSYKTGAAFFLLSKTIGAAARLYVVAIVLQAFVLEKWGVPFALTVI
                     IIIAMIWMYSFRSGIKTIVWTDLLQTLCLLTALVLIILELSKSLQPESGSVWGLMKAS
                     PYMKMFEFEDWRSRQHFVKQFFSGIFIALVMTGLDQDMMQKNLSIPKLDKAKKNIYTY
                     GFAFIPVNFLFLSLGVLLLLFAQKNQLELPVSSDDILPVISTGPYLSPATGIFFIIGI
                     ISAAFSSADSALASLTTSFCVDILDRSDDVRIRKWVHLLISMLFLLIILIFKAVNKRS
                     LIDAIYVIAGYTYGPLLGLFTYGLFSKRPVKDKWVPYIALSAPVLSALFQYISTRYWS
                     YAMGYELLMFNGGLTLLMLWLSGKMVSNQTVGGKKVGSKTAGVCIKHNSRNN"
BASE COUNT         3870 a         3883 c         3542 g         3807 t
ORIGIN      
        1 acccgtggca tcctgaaaaa tagcgtagga cgaattcaag ccgcgacctt cgagaaacat
       61 ggtgcgcagc gaggtgcccg aacccttggg tgccaccaga ataacatcga tgtccttggg
      121 aggaataatg ttggtacgat ccttataggt gatggcaaaa ccatgcgaaa aatacagggc
      181 tttgcctgct gtcaggtgag gtttgattct gggccagacc tcaatctgag cagcatcgga
      241 aagcaaatac tgtatgatgg ttcccctgcg gcaggcctct tcgatttcga acagggtttc
      301 gccgggtacc caaccgtcgg ccacagcctt atcccaggtg gcaccgcctt tgcgttgtcc
      361 cacaattacg ttgaaaccat tgtcccgaag gttcttggat tgtccggggc cttgtacacc
      421 ataacctatc acggctatgg tttcgttttt caatgtttct tttgcttttt tcaacgggaa
      481 ttcttccctg gtgatgacat tctccattac accaccgaaa ttcattttag ccataaatat
      541 aaatttactg ataagacatt agttgtttcc attaaatact tcttttctgc gagcttccat
      601 ctcttccaga aaattactca gccgttccac cgtcgatctg gtgatggcaa cccgtccgga
      661 acgaacaaat tgcagaattt tgtttttgtt taaagcgtca aacatagcgt cgatttcggc
      721 tttatgtccc gcctgctcaa taacggtgta gtctttggtt acttccagaa tacgtgcatt
      781 gtatttccgt accagagttt cgatttcgtt gctcgacatc aaacttaaag taggcacttt
      841 atagagtgca atttcttgaa aaatgatctg gtcgtcggta taataatagg cctttagcac
      901 atcaatgcgc ttttcgatct gcaaaacaat cttgcgtatg gtttcccggt cggtgttggc
      961 ggtaatagtg aatttatgta cgcccggtat ggccgaacgc gaaaccgtca ggctttcgat
     1021 gttcacttgc cgtctggtaa acacaatggt cacctgattg agcaaaccca cctgattctc
     1081 ggaataaacc gtaacggtat ataaagtttt ttccataatg tcagctcttt aacatgattt
     1141 ctgtgataca ttttccggca ggtaccatcg gatagacgat acctttcttt tcgaccataa
     1201 cttcgagcac ataagccgaa tcggcggcaa tcatttctct gacggcctcc gcaagatcct
     1261 tacgctgttc cactttgcgt cccttaatgc cgtaagcggc agccaaagca ataaaatcgg
     1321 gattttgtat cgaagtggac gaatagcgct cctcgtagaa caattcctgc cattggcgca
     1381 ccatacccag gtaattgttg ttcaatatga tcaccttgac cggtatttta tactccataa
     1441 tcgtgcctaa ttcctggatg gtcatttgaa tgcccccgtc gccgctgatc acaaatacct
     1501 tggattcggg cgaacccaat gccgcaccaa cgccggccgg caggccgaag cccatggtgc
     1561 ccagaccgcc ggaggtaact attttccgcg tgttataggc gctgaaataa cgggcagcag
     1621 taatctgatt ttgccccacg tcggtcacca ctatggtgtt ttcgccggtg ttgtccgaaa
     1681 ggcatttgac cacttcggcc atggttagaa taccgttgtt cggatacaaa tccttgctta
     1741 cgacttcccg gtattcgcaa agcttgtgtt cttcgaaact ctctatccat tccttgtgat
     1801 cggccttgtc gagaagctcc gtaatggcgg gcaggctctt tttgacattg cccagcacgc
     1861 ccacttcgac ctggacgttt ttattgatct cggaggggtc tatgtcaaaa tgaataactt
     1921 ttgcctgcgg ggcatattcg ctcaatttcc cggtaacccg gtcggaaaaa cgcatcccta
     1981 tggcgataag cacatcgcat tcgttggttt ttttgttggg agccacgttg ccgtgcatac
     2041 ccaccatacc catgttgagc ctgtaatcca cgggcataac gtcgagcccc agcaaagaag
     2101 aagcagccgg aatatcggct ttattcaaaa agttgaacaa ttcctgttcc gcccggccga
     2161 gcaggacgcc ctgccctacg acggcaaaag gttttttcgc ttgattgatg agttgggcag
     2221 cccgggctat tttatccgga ttgagctcag gctccggctg gtaactgcgg atgtaacggc
     2281 atttctcgta gctgaattca atgagttgat tttgggcgtc tttcgaaagg tcgagcacta
     2341 ccggcccggg acgtccttcg cgggcaatat agaaggccct ggcaacggcc caggaaatgt
     2401 cttcggccct gcgaatctga taactccact tggttatggc ttgcgtaatc cccagcacgt
     2461 cggtttcctg aaaagcatcc gaacccaaag catctaccga agtttgtccg gtaacgacca
     2521 ccaagggagt gctgtcgagc atagcgtcgg ccactccggt aacgatattc gcagcggcag
     2581 gtcctgaagt aacaatgacc acaccgacct tgcccgatac cctggcataa ccctgagcag
     2641 cgtgggcggc tccttgttca tgacgggtaa gaatgtggtt gagcgccagt ttttccttgt
     2701 gggtatataa agcatcaaac accggaataa tggctccccc cggatatccg aaaatagtgt
     2761 ctaccttttc ctctttaaag gcaaacaaga gggcttcggc ccctgtgatt tttttctttt
     2821 ccatttttta atcctcttca atgattcgaa cagctccttt gtcggccgaa ctgaccatgc
     2881 cggcataggc tttcagcgct ttggaaacct tgcggtcgcg cttgggtttg aaggctccgg
     2941 cggctttctt ttcttcctcg agccgtcgtt gattcaactc ctcggcaccg attctgagat
     3001 ttatgctacg gtgtggaata tcaatgtcaa tgatgtcgtt gtcgcgaatc agggccacag
     3061 cgccacccga agcggcttcg ggcgaaatat gcccgataga cagacccgat gttcctccgg
     3121 aaaaacggcc gtcggtgatc agggcacagc ttttgcccaa acctctcgat ttgatgtacg
     3181 atgttggata cagcatttcc tgcatacccg gacctccttt gggcccttcg aacacaatta
     3241 ccaccacatc gccggcttct accctgccgt cgagtatgcc gtcgcaggcc gcttcctgtg
     3301 aatgaaagac tttggccttg ccgctgaatt tccagatgga ttcgtccact ccggctgttt
     3361 tgaccacaca gccgtcctgt gcgatattgc ctttgagtat agccagtccg ccgtcgcggg
     3421 tataagcatg ctcaatatca cggatacagc ctaagctgcg atcggtatcc aattctttat
     3481 atacggcctc ctgcgaagcg agggtattat tgaatttacc tccgggagcc gatttataca
     3541 aggccagggc ttccggcagt acgttggcgg aacggattga gtacttatcc atggcttctt
     3601 tcagggtata accgtccact ctgtaaaccg aattgtctat caaagcggct tggtccagct
     3661 cgtccataat agagagtatg cctcccgccc tgtttacgtc ctgaacgtgg taatgactgc
     3721 tgggagccac tttgcacagg cagggaattt tacgcgacag cgcatcgatg tcgtccatcg
     3781 taaaatcgac ctcggcctct tgtgccaaag ccaataaatg cagtatcgta ttggtggaac
     3841 cgcccatagc tatatcgaga gccatggcgt tcatgaatgc tgctttgctt gcaatggaac
     3901 ggggcagtac acgttgatcg ccgtcgcggt aataggcaaa cgtattgtga actatctgcc
     3961 gggcggcctg ttggaagatt tttttcctgt tgacatgggt agccagcacg gtgccgtttc
     4021 cgggcagcgc catacccaag gcttcgttga ggcaattcat ggagttggcc gtaaacatgc
     4081 ccgaacaact gccgcaaccc gggcaggcca cagcttcgac ggcggccagt tcttcgtccg
     4141 acaccttggg atcggccgcc tgcaccatag catcgatcag atccaggtaa gtatcgccca
     4201 gcttgccggc ttccataggg ccgccggaaa caaacaccga gggaatgttc atcctcaggg
     4261 cggccataag catacccggg gtaattttat cgcaattgct gatgcaaatc atggcatcgg
     4321 ctttgtgggc attgaccatg tattccacgc tgtcggcaat caaatcgcgg ctgggcaggg
     4381 aatacaacat accgtcgtgt cccatggcta tgccgtcgtc tatggctatg gtattgaatt
     4441 ccgccgcgaa acagccggct ttctcaattt cggctttgat ttcctgcccg atgttgtgca
     4501 gatgcacatg tccgggtaca aactgagtaa aagaattgac tatggcgatg atcggtttgc
     4561 ccaattgttc ctttttcatt ccgttggcca gccacagggc tctggccccg gccattctcc
     4621 ggccttccgt gcatacggcg ctgcgtaatg gatgtttcat ttttgtagca attaaccaaa
     4681 aaaaaagcct ttccgtttca tgggaaaggc ctatactata tttctgtgca cacttattaa
     4741 catacctttc ccgatcccga agccaccacg actaggataa tcactaggac agatactaca
     4801 agtgcaaaca tctttctttg tttttgtggg gcaaatgtag acttaatttt tatatgtacc
     4861 aaccaaacgc ccgttttttt tattttttct gtttttcaat gggagttggg gctgttttcg
     4921 cgcggaatcg ctttctgccg gaatctaaac gctcataaaa caagcaggcg gaccgggccg
     4981 aaaacagcct gccgctttgg cggcagcggc ttgccgaagc aaatttgggg tagcaacccg
     5041 gcggagggca gtttggccgg gggtggtttg gcggcagcgg cttgccgagg gaaattgacg
     5101 gtggcaacct gacggagggc ggtttggcga gaccggtttt atgaatgatg atagggttct
     5161 cccctcagaa tggtcatggc ccggtagagc tgttccacaa aaaacacccg caccatctga
     5221 tgcgaaaaag tcattttcga cagcgaaagt ctctcgggaa cccgtttata aacagattca
     5281 tcgaaaccat aagggcctcc gcatacaaaa accaggttac gcaaaccggc gttcatcttt
     5341 ttttgaaggt aagcggcaaa agcttccgaa gtgtattcac ggcctctctc gtcgagcaaa
     5401 accagggtat tctccggttt aatccagggc tccagcaaag ccgcttcacg ttgcttttgc
     5461 tcctgttcgc ttattttgcc cgatcttttg agttccggca aataaacgat ctcgaaggtt
     5521 aagtaatgct tcaacctttc ctcaaacagt ttaatagcct tgcgggcgta atcttcgccg
     5581 gtcttacccg acaacaataa aatgactttc atgcctgcaa aaaaaggcaa ttctgcacaa
     5641 atagcgaagc acttgcaggg ctttttgaaa taaagaccgt atttttgcga tgaattcccc
     5701 gaagaaataa accattatgg cctcattaga agattttatc cgcaatgctt ttgccgaaga
     5761 cataggcgaa ggcgaccaca ctagtttgag ctgcattcct gcttccgccc ggggcaaatc
     5821 catgttgctg attaaagaag cgggagtcgt ggccggcgta cgggtagccc tggaagtatt
     5881 caggctcttc gacccgcaat tgcaaaccac cgtatttata caggacggcc aaaaggtgaa
     5941 acccggcgat attgtttttg aaaccgccgg cagcgttcgt tctcttttgc agactgaacg
     6001 actgatgctc aacattatgc aacgaatgag cggcatagcc accaccaccc gtttgtatgc
     6061 cgaccgactg caaggcttac acaccaaggt gatcgacaca cgtaaaacca ctcccggcat
     6121 gcgcctgctc gaaaaggaag ccgtagtact gggcggaggc agcaaccatc gcatgggcct
     6181 ctacgatatg attttgctca aagacaatca cgtcgatttt gcaggcggca tagccaaagc
     6241 cattgcagcc accaggggtt atctgcagga aaaaaagctc gacctgaaaa tcgaaatcga
     6301 agttcgttcc ctggacgaac tggagcaggt gcttcaatgc ggaggagtgg accgcatcat
     6361 gctcgacaat ttcgatcccg ccaccacccg caaggccgta gaacgcatag cgggacgtta
     6421 cgaaaccgaa tcttcgggcg gcatcaccct cgataccatc cgggcctatg ccgaatgcgg
     6481 cgtggatttt atttcggtgg gcgccctcac ccactccata cctagcctcg atatgtcgtt
     6541 caaggctgta ctatgaatta tacgctgatc cgttcccgtc gcaaaaccct aactttgcag
     6601 atcaaacccg acggcagcct cttggtgcgc tgccctctga gactgaacct gcgcgaagtg
     6661 gagcgcttta ttcaggccaa acaaccttgg atactctcca aacaacaact gctcagggaa
     6721 aatttcgaaa aacggcaagc cttccgcctg cacccgggca tggaactccg ctacctgggc
     6781 aagacttatc ccctgcgtct ttccgaaaac cgaaaaagcg gattcgactc ccggggtttt
     6841 tacctcggca acgaggttcc cgaagaaaaa atccgggcca cggtggtaaa aatctataaa
     6901 gaactcgcca caacacatat tacagccaaa gtagagcatt acgcccgtct gtacaagctc
     6961 aaatacaccg acgtacgcat aggttcagcc aaaacccgct ggggctcgtg cagttccaaa
     7021 aaccggctca ttttcagctg gcgactcatc atggcagacg aactctgcgt cgattacgtg
     7081 gtactgcacg aattggccca caccctggaa cacaaccaca gccaccaatt ctggacaaaa
     7141 ctcggccgaa tgatgcccaa ctacccggaa gcccgccgac aactgcggat ttttggcaga
     7201 cagttgcagg cggaggattg gtaaaaccta cttactgaaa accttacctg agagctattc
     7261 atcgctgccg gcaagtccgc aaaagtcggg tgtttttaat cctcgtggac aattcgctta
     7321 tttatcggta ccattttagg gattgggggt ctgaaaattt gataatttta cgctcggaat
     7381 acgaaaccta ttacttatta cattcaacgc tatgtcaaca ggattttata cgataccgga
     7441 aatcaggaat gagccggtaa aaacttatgc gccgggtacg cccgaaagag ctgctttgaa
     7501 aaaaaccctg gccgaactca ggtcgaaaga agcggatatt cccatggtga tcggaggccg
     7561 ggaaatccgc accggtaaaa aagtacggat ctttccgccc cacgaaatca aacataccct
     7621 gggctattat taccagggcg acggcagtca tgtgcaaatg gctatcgacg cagccctggc
     7681 ggcccgcgac aaatgggcgg ctatgtcctg gcaacatcgt tctgccattt tcctgaaggc
     7741 tgccgacttg ctggccggcc cctaccgtca aaaaataaac gccgccacca tgctggggca
     7801 atccaaaaac gcttttcagg ccgaaatcga tgccgcctgc gaactggccg actttttccg
     7861 ttttggtgtg cgttgcgtaa cggatattta caaaatgcaa cccgaatcct cgcccggtat
     7921 gtggaattac aacgaattcc gtccgctgga aggctttgtg tttgccctga cgcctttcaa
     7981 ttttacttcc atcgccggca acctgcctgc agccccggcc atgctgggta atgtggcggt
     8041 gtggaaaccc tccaaaacag ccatttattc ggcggccgtt attatggaat tattccgtga
     8101 agccggtttg ccggacggcg ttatcaacct ggtatttgcc gacggaccta cgacttcgaa
     8161 cgtggtgttg aattccccct attttgccgg catccacttt accggctcta ccggcgtatt
     8221 ccaaagcatc tggaaaacca taggcgaaaa catctataaa tacaaatctt atccccgcat
     8281 cgtgggcgaa accggaggta aagattttat ctttgccgat cccaccgccg atccccaggc
     8341 cttgaccatt gccctgctgc gcggcgcttt tgaataccag ggccaaaaat gttcggcagc
     8401 ctcgagggca tacattccgg ccggtttgtg ggaagatatc cgtgaacgct taggcaaaga
     8461 gctggccacc gtcaaaatgg gcccgcccga agactttagc aactttatca acgccgtgat
     8521 tgacgaagct tctttcgaca agctcaaggg ctttatcgac cgcgcacgga cggacgccga
     8581 tgccgaagtg attttcggcg gcaactgcga caaatcggtg ggctatttta tcgagcccac
     8641 ggtgattttg gccaaaaagc ccgattacat caccatgatc gaggaattgt tcgggccgat
     8701 acttaccatt tacgtgtata aagacgaaga actggatgcc accctcgatc tgctggatcg
     8761 ttcctctatt tacggcctga ccggagccat cttttcgcag gacaggtata acatcgagaa
     8821 aatgaccaaa aagctggtga acgcagcggg caatttctac atcaacgaca aacctaccgg
     8881 agctgtggta ggccagcaac cttttggcgg agccagaggc tcgggcacca acgacaaggc
     8941 gggttcggta tttaatttct tgcgctggac atcgatacgc accatcaaag aaacctttgt
     9001 acctcccacg gattacatgt atcccaattt cttgcccggg gactaaaacc ggcattgctt
     9061 attgcaaaaa aaatcgttcc tttgttctct ttgtcgaagc agcttcgctg ccttggcaag
     9121 ttgtttttaa ccataaagct atgaacaaag caatcagaaa aatcggactt gttgcccacg
     9181 acggtatgaa acaagacctg atcgaatggg ccgtatggaa ctcggaacgc ctgatcggac
     9241 ataaatttta ttgcaccggc accaccggca agctggtaca ggaagccctc agcagaaaac
     9301 accccgatat cgactgggat attaccattc tgaaatccgg cccgctgggc ggcgatcagc
     9361 aaatgggctc ccgcatcgtg gagggcgaag tggattattg tttcttcttt accgactcca
     9421 tgactttgca gcctcacgat accgatatca aagcactgac ccgcctggcc ggtgtcgaaa
     9481 acatcgtgtt ttgctgcaat cgctctactg ccgaccacgt catcagcagt cccttgtttt
     9541 tggatcccgg ctataaaccc attaagaagg actacagctc ttataccaac cgcctcaaag
     9601 accgggatct gataagcaaa gcggtggaaa gcagccgttc ccaaaaaaga cgtaagaaac
     9661 gctctaaacc cggaaacgaa taatacagta ataaaaacga tcgccatgaa aaagtatttt
     9721 gttgttattt cagttgtcct gcttatgatt tcctgcaaaa aaacggccga tacaaacctt
     9781 cccgaaagca ccgttcagca ggccctgact gcttgcaccg cgcaatctga actgccggat
     9841 gatctcatcg aaaaaggact gcgtcaatgc gccgggctct ggactccggc agacggcagt
     9901 cccgaagagt ttgtggagtt ttgcaaaatt cacctctgtg ccgacgctca acaaaaaagc
     9961 cttttgttcg aacgtatggc cgaccatttc gaaaccctgc tgggacacca aaacagggtc
    10021 agagtggaat taatgaggcc cctgcacgaa acgggctacc aaaacctgcc ggtcgatcag
    10081 ctttttggag cttacgacaa tgctgcccat ttcgaagagg atatgtacgg cagcaaaatt
    10141 gcttttgtgg tggctttaaa ctttccttat ttcagcctcg acgaaaaaac agccctggcc
    10201 gaagaatgga cttccgagga atggggctat gcccggctgg gcgatttctt tattgagcgc
    10261 attcccgcct ccgtcaaaca ggaaatgacc ctggccgcca ccagagccga ggattatatt
    10321 tcgaactaca acatccacat gggcagcctt gtggatcggg gcgaaaagct tttcccggac
    10381 catatggtgc ttatctctca ctggggcttg agggacgagc ttaaatcgaa ttacgccgac
    10441 aaggaaaggg gtctgcaaaa acaggaaatc atctaccggg tgatgcaaca cattatcgaa
    10501 cagagtattc cccttcaggt aatcgacaat cccaactatg attgggaccc tatcaacaat
    10561 aagttatatc tggaaggcaa ggaaatcgcc gttgaagctg agcccgatac ccgctacgag
    10621 cattttctga atctctttaa aacctacaaa gcacaagaca actacagtag gcaatatccc
    10681 tcttacattg cccgctattt tgaaggcgaa ctggaaattg ccgaaaaaga ggtggaagct
    10741 ctttttgtag aactgctctc gtcgaagcag gccgccctaa ccggccaatt gatagcacaa
    10801 aggctgggcc gcaaactgag gccctacgac atttggtacg acggcttcaa gagcagaagc
    10861 agcatcgacc cggctgtgct cgataagata gtgctcgata agtatccctc tttgcaggcc
    10921 ttcgagcaag acctgcccgt cattctcgta aaaatgggtt tcagtaagca aaaagccaag
    10981 gaaatctgcg ctcaaatcga agtcaatcct tcggtgggct cgggccatgc ctggccggcg
    11041 gccatgaaaa acgacaaaat ccatctccgg gcccgcttcc gcgaaaacgg cgtagattac
    11101 aaggcctaca acattgccac ccacgaattt ggccataacg tggaagaata tatttccctg
    11161 tacgatgtgc ccaactactt cctgagttcg gtgcccaaca atgctttcac cgaggctttg
    11221 gcctttgtgt ttcaggaaag agacctggac ttgctgggtt taaaacagca agatcccctg
    11281 gccgccagcc tgaataccct cgatttgttt tggagttctt acgaaattat gggcgtggcg
    11341 ctggttgata tgaaggtgtg gcgctggatg tacgaaaatc cctcgaccga tgcagtccaa
    11401 ctgaaagaaa acgtcatccg catagccaaa ggagtctgga acgactatta cgctccggtg
    11461 tttggtatcg aagaccaaaa catactggcc gtttattcgc atatgatcga aattcccctt
    11521 tatttgccgg cttaccccct ggggcatttg attcattttc agttgaacga acacttcgaa
    11581 ggcaaagaca tgggagctga aattcaacgc atctatgctc tgggacgcct tagcccccag
    11641 tactggatgc aaaaagcggt cgggtcgaaa ctctcggcgg agcctttgct caaggctact
    11701 tcactggcct tggaacaagt tatgcaataa cagattattt tttttaaaaa aagcccaacc
    11761 tccttggaca ggctcaggtt tatcaataca attacagcca tttggtccaa aaaagcatct
    11821 cgggacggcc ggcttcgaag cccagcccgc tgtacacttt ttgagcggcc ggattgtcgt
    11881 tgcgtacctc gagtgtgagt ttgcaatagt cgcgttcttg cgccaaacgt accaattctt
    11941 tgatcatcga ttgaccatag ccccggttgc gctgcgtttt atgtaccact acgtcgtgaa
    12001 tataaatgta aggtttaagc ctgaaagtgg aataattcac aaagaaagtc gtcataccga
    12061 tgtattctcc actcagactc atcagtagga cttcgaccgt gggatgagta ctcaaatcgg
    12121 ccagcaactt ttgctgttct gcaccgtcga ggggcggata attgcccatg ggatcggcca
    12181 tgtaatgatt caacaaagat agaaacgctc ctgtctgcct gctgttgtcc cagcggcata
    12241 attcaaattg cagctccgct cccataatcc acttgttata agcttgttaa gataagtact
    12301 cgtgtccgcc cggcttcgga caggtgtaag tcttcacgcg ttaatagccc gcaaagatag
    12361 gtattttaca gctttttcac tctgcagaaa acgcagaaaa atcgcattta aagcccgcaa
    12421 atgtaggtaa tttaccgaat acgttttgca ttaaagcaca aaactctatc tttgtagggt
    12481 ttaaacctta tcccatgaaa cgaaactccc tgttcaaaga caattactgg ctctttatcc
    12541 cctatttgct gatgctcgct atcagcgttt ttttcattct tttttattcc aaggctgaga
    12601 tacatatctg gatgaaccaa caccacagcc cctttgccga tcttctcttt aaatacgcct
    12661 cttacatggg cgaaggttgg gtactgacac ccatttgctt gattttattg tttgtacgct
    12721 ttcgttacgc cttcatggcc ataggctcca gcattttggc ccctttgttt acccaatttt
    12781 tcaaacgggt ggtttggccc gacagtccca ggcctaaagt ttttttcgag aacctctacg
    12841 atttacatgt ggtcgatgga gtgcatttgc attcggccca cagctttccc tcgggacacg
    12901 ccacgggagc ttttgccgtt tttatggtac tcgctttgat tgttaaaaag cctttttgga
    12961 aagtattttt tctgatattg gccagtctga cggcctattc gagggtatat ttatcccagc
    13021 attttttggt ggacgtcagt gtgggttccc tgataggaag ccttacagcg ctgctctgtt
    13081 attgttggat gatgcgctat tccaaaggct ggctggataa atcctttaaa atctgcgtta
    13141 aataggtaag taaggccgtt ctgccctgtt ttttttcgga cgaactcaaa aaaagcggag
    13201 gcaggctttc ccagctttcg agcaagcgat tgcgatagtt ttcgatgttg agattcaact
    13261 cggatttttt gcatttatct attttggtaa acaccagcgc aaaaggcacc tggttttctc
    13321 ccaaaaactc caaaaaatca agatcgattt cctgtggccg atgcctcgag tcgagcaaaa
    13381 caaacacaca cagcaaattg ggtcttttca gcatataatc gctgatcatg cgatgcaact
    13441 gctccctttg tttgagggaa accctggcgt aaccgtaacc gggcaaatcc accaaatacc
    13501 aggcttcgtt gaccagaaaa tgattaatca gcaaggtttt acccggagtc gaggaagtct
    13561 tggccaagtt tttttgattc agcaacatat tgatcaggga agatttccct acattggaac
    13621 ggccaataaa ggcaaattcg ggcaaagaag tcttgggaca attttcgacc gaggtattgc
    13681 ttacgacaaa gcttgcgctg ttgatttcca tcttagttat ttcttgaatt gtgttttata
    13741 caaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcttg cgctgttgat
    13861 ttccatctta gttatttctt gaattgtgtt ttatacaaac gccggccgtt ttgctgccca
    13921 cttttttgcc gccgaccgtc tggttactga ccattttgcc gctgagccac aacatcagca
    13981 gtgtcagtcc gccattgaac atcaataact cgtaacccat ggcataagac cagtaacgag
    14041 tggaaatata ttgaaacaat gcactcaata caggtgcact caaagcaata taaggcaccc
    14101 acttatcctt gaccggacgt ttgctgaaca aaccgtaagt gaacaaaccc agcaaaggcc
    14161 cgtaggtata gccggcaatt acgtatatgg catcaatcag gctgcgcttg ttcactgcct
    14221 taaagataag aatgatcagc agaaacagca tcgagatcag caaatgtacc catttgcgta
    14281 tacgaacatc gtcactgcgg tcgagtatat ccacacaaaa cgaggtggtg agcgaagcga
    14341 gggccgagtc tgcgctcgaa aaagcagccg agatgatgcc aatgataaag aagataccgg
    14401 tggccggact taggtagggg ccggtggaaa taaccggtaa aatgtcgtcg gatgaaaccg
    14461 gcaattccag ctgattcttt tgagcaaaca gcaacaacaa gacacccagg ctcagaaata
    14521 aaaaattcac cggaataaag gcaaaaccgt aggtataaat atttttcttt gctttatcaa
    14581 gcttgggtat actcaggttt ttttgcatca tgtcctgatc caaacccgtc atgaccaatg
    14641 caatgaaaat gccgctgaaa aactgtttga cgaaatgctg cctgctgcgc cagtcttcga
    14701 actcaaacat tttcatataa ggactggcct tcatcagccc ccagaccgaa ccgctttcgg
    14761 gctgtaaact cttggacagt tcaagaataa tcaaaaccag ggctgtcaat aaacagagcg
    14821 tttgtaacaa atcggtccac actatggttt taataccgct acgaaaggaa tacatccaga
    14881 tcatcgctat gatgatgatt actgtaaggg caaagggcac cccccatttt tcaaggacaa
    14941 aggcttgtaa aacaatggct accacataca gacgggcagc cgctcctatg gttttggaca
    15001 agagaaaaaa ggcggctccg gttttataag aatacttgcc gaagcgctct ttcagataga
    15061 cataaatact gggagaattt aggcgataat aaaccggcag caaaacattg gcaatcaaaa
    15121 tatagccaac gaaaaagccc atcacggtct gcatgtacaa aaagctgttg tcgcgtacca
    15181 tgcccggaac cgaaacaaaa gt
//
LOCUS       DAOSGL010000016        13565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_3773_length_13565_cov_3.745892, whole genome shotgun sequence.
ACCESSION   DAOSGL010000016 DAOSGL010000000
VERSION     DAOSGL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13565
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3773_length_13565_cov_3.745892"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PKY68_01175"
     CDS             complement(<1..876)
                     /locus_tag="PKY68_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOH23682.1"
                     /translation="MTTKKHYWNEYQKEIAGDKYFYERSCIRQTFFPGSETAFLRILK
                     EELGKNILDVENQHTCTGIGFYSDIVPLETTMTIVARLFSLMTEAGYENYVPSCVTSF
                     GVFSEIVDLWEHQPDMLEKARNILLETTGRTFELPKNIAHPSDIIYKFREELKEKMRY
                     RLVNRFTGKPLKVVEHIGCHYAKIFPDKGVGKAEFPHVLAGMIESWGGEVVDYPERRH
                     CCGFGFRQYVVRANRGYSVANSKKKFESMQPYEPDFIVTNCPGCTMFMDKWQYTIAEM
                     EHKTYDKKGYGIPVLT"
     gene            complement(873..1553)
                     /locus_tag="PKY68_01180"
     CDS             complement(873..1553)
                     /locus_tag="PKY68_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOH23683.1"
                     /translation="MGNLYNLLTQDVRFEEALNSCMNCGICTAVCPAAEFYNYDPRKI
                     VDTIQRRNEDEIEALLRSETIWYCGQCLSCKTRCPRGNTPAVLIAILRSVSQKMGYFT
                     ESEKGLQQFAISKKIAQTILDTGYCVHPDTMSYEQHPEQGPVWKWYTKYIEEIAPRFG
                     ANYHGEGPGALRNIRKETLEEINKIFEATGGKELMRKVETGAKNKTNIRNDEDLLNLV
                     YNRNTSKE"
     gene            complement(1574..2803)
                     /locus_tag="PKY68_01185"
     CDS             complement(1574..2803)
                     /locus_tag="PKY68_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOH23684.1"
                     /translation="MKSQLKCVKCGYISPGFAAWFEQDQTCPKCGSKHSEAEYQADYT
                     QLEALYKQGADSFWHYFDFLPLENRDNIISCKEGAIPIESWDFLEDFAREHYGIECRA
                     LVYRNDLNGGTQTFKDVAGSMAASLFKEHGVKQYCIASTGNTATAYAKYLSLAGVAFK
                     VFVPAGANEDSVQEILGFGQEVVRSQGDYARAKQEAADYARQNKVLISAGNFDPIRVE
                     AKKTMVFEFMRQLSKMPEVYIQAVAGGTGPIAIDKGVREIEKYYPEVVLPRMLLVQQD
                     LCDPMVQAWEQAVKEGFPENYHKNYPVIDNPQTKVSILSTGNPGMYPVIAPIVRKTGG
                     SFLRISEASLPALAREVYDQQGVLLGPASIVCLAGFYRALEEKRIHQGDTVLINCGEG
                     MQRAAAFHKEVLAVAGK"
     gene            complement(2800..4062)
                     /locus_tag="PKY68_01190"
     CDS             complement(2800..4062)
                     /locus_tag="PKY68_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH23685.1"
                     /translation="MKDIFERIQATPGPLGQYQGAADGYFMFPKLEGEIQPIMRFNGR
                     DVLVWSLNNYLGLANHPEVRKADADASKEWGLAYPMGARMMSGDTVHHKKLEQDLADF
                     VGKEAGYLLNFGYQGMVSIIDTLLSRRDVVVYDSESHACIIDGLRMHFGKRFVFQHND
                     MDSFRAQMESATALAEQQRGGILVITEGVFGMAGDLGKLDEIVAMKKDFKFRLLVDDA
                     HGFGTMGATGAGTGEHLNCQDGIDIYFSTFAKSMAGIGAFVASDRAIVDHLRFNMRSQ
                     IFAKSLPMPMVIGAMKRLELLKTKKELRENLWIIVRALQNGLKEKGFDIGRTQSPVTP
                     VFLKGGVNEGSNIVVDLRESYNIFCSIVVYPVVPRDVIMLRIIPTAAHRMEDVERTIN
                     VFSEIKKKLETGEYKREIPNMNLVNKQK"
     gene            complement(4279..5235)
                     /locus_tag="PKY68_01195"
     CDS             complement(4279..5235)
                     /locus_tag="PKY68_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOH23686.1"
                     /translation="MTIQILLLLLGFVLLIKGADWLVDGGAALARKYHVSELAIGLTI
                     IAFGTSMPEMVVNVFAAYKGHADIVYGNILGSNIFNLLGILGIAGLISPLIVQASTVW
                     KEIPLSLLAVVILYLLSNSIFTQNELLSRWDGIILLLVFAFFLYYVFRQLKEENTVSV
                     EEQHKEFTIFKIIVFIVLGLALLVVGGRLVVNNAVKIAEFLGISQTIIGLTIVAVGTS
                     LPELATSVVAAIKKNNDIAVGNIIGSNIFNIFFIMGVSSLIRPIEYNTKFNFEMYFLS
                     FGTILVFIAMFSGKKRRLDRWEAGILLLMYVGYTILLINEQI"
     gene            complement(5261..6190)
                     /locus_tag="PKY68_01200"
     CDS             complement(5261..6190)
                     /locus_tag="PKY68_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOH23687.1"
                     /translation="MSQLRIKFLLFFLPGLCLFFNAPAQEFIPLWPEGKMPNSKGLTL
                     EHKEERQRITQVKTPGMYAFWTSNEDNKGSAVLICPPGGYQKLTYHLAGFQWAKWFNT
                     MGVNAFVLVYRLPNSPDLIEREKGPIQDAQRALKIIRNMASVRNIDPGKVGIVGASAG
                     GHLASTLGTHFEDFSLIGDSIDRYSVRPDFMVLISAVINMGDFAHEGSVQAFLGADAG
                     KEKRDYYSNEKQVTEQTPPTFLVHARNDNTVLVQNSIQFYNAMLEKGVDGSLHIFPYG
                     RHSISLKNESAMLNYWTSLCENWLYEMGLLKQK"
     gene            complement(6228..7319)
                     /locus_tag="PKY68_01205"
     CDS             complement(6228..7319)
                     /locus_tag="PKY68_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23688.1"
                     /translation="MRALLLVLFAGFDLAASAQANRADYTGYTNRTNPPAKLKLLSAH
                     PREVIGPEHPDVILSRNFSGFETGQVIKMDGTYHMFVNEMFVRPFRDLRISYWTSKDA
                     LNWERRATIRESIPGRSPYNPRSEVWVTGVEFNEDENAWNIFYVAYRAGDETKGEIAR
                     SDYSGRIWRAKSVIPGIKGIAGPYADMGIVLQPGELSQAWEGQQAVASFNPYKVGDTW
                     YAFYDGHNYIPQGGWPTGMARAEKLEGPWTRMTEDFNPLPIVDEFMENTVITQLKNGK
                     YLAVFDSFGDREIGYSLSEDGLNWSKETRIKVQFEDRAWVKDGNHSLRTPLCAIEEDD
                     GTFTVIYTGLMDLREEAFYAVGKCTLAWE"
     gene            complement(7492..8943)
                     /locus_tag="PKY68_01210"
     CDS             complement(7492..8943)
                     /locus_tag="PKY68_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOH23689.1"
                     /translation="MKTIPHRFIPCFPVLLLLMGCTGDVFSSRDGKHQNSPMSIHAST
                     ENAQTKTLLSGEPGDPVRSICFLPLDSIAFSADLGEFKPFVNTSSDTLPYATFSGDLI
                     DADMYFAFYPWKLALNLNRDTLLTLKLPHTQQYKAGGFASGAYPMVSWSYDTNLHFKN
                     LCGLLKLNLCGDVSVSSLSLCAPGLAGVAGVDMRYQGVPQLIMPTPPAYDTLTLDCGE
                     GVQLDDEQATAFYFVLPPALYESVTLIVRTTDHQQMTETTQNLRIVRSSITPTSVIEF
                     DSDDNPNPPGNTVLDIQGNVYKTVAIGNQIWMAENLKSTLLNDGTEIPHITDNSSWNT
                     LTTPGYCRYNNDPALYSDYGLLYNWFTVETGKLCPQGWHVPSHAEWTELTDYLGGESI
                     AGNKLKESGTGHWAGSNPGSTNETGFTGLPGGLRNNYGVFQYLGYGGFWWSATEGGGG
                     VWYRYVYFDDSAVYAYDNGLKELGFSVRCVKSD"
     gene            complement(8976..10946)
                     /locus_tag="PKY68_01215"
     CDS             complement(8976..10946)
                     /locus_tag="PKY68_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOH23690.1"
                     /translation="MRSFVKHILNILLLVSMAACLEYENFDGVMWQSTLPTLSTHVPS
                     NITATSAVSGGTILSDGGSPVIERGICWDYSRNPTINTVYKTEEGGGTGEFTSTMTGL
                     PPHTKVYVRAYATNANGTGYGNEQVFNTEAEEFGTLTDIDNNTYRTMEVGAQIWMVEN
                     LKTTRFSNGDEISYLADNTDWTSYALAGWPASCSVNNSSSNDYEYGRLYNWYAVNDGR
                     KLCPNGWKVPTHEDWAALADLLGGESVAGGKLKETGTAHWIAPNVGATDDIRFSALPA
                     GFRNQEEGVFLNLGYRAYFWSSNGWDDEWARMRYIDHDSTWFRGNFVGKKGGGYSVRC
                     IKDESTSIQISTVAAYSITYISAKSGGRITSDGGSAVTARGVCWNNYGYPTTGDNKTE
                     DGTGTGEYTSNLTGLQPNTTYYIRAYATNAYGTVYGDELSFTTPAPQYGSISDIDGNS
                     YKTVIIGDRTWMVENLRTTRFVTGEAIPEISDQAEWSTYDSQTLPAACNVENDAGYVS
                     VYGKLYNWHAVDDDRKLCPEGWEIPSHDDWVSLMDYLGGEALAGGRLKEAGTSHWAEP
                     NTGATNDSYFSALPAGYRMSDGNFSSLGFGAYFWTSDEYESPTARLVYMDKDNASFFH
                     KYIVGKGYGFSVRCIKDDDEGEDLDTQDEHDW"
     gene            complement(10950..12353)
                     /locus_tag="PKY68_01220"
     CDS             complement(10950..12353)
                     /locus_tag="PKY68_01220"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOH23691.1"
                     /translation="MVKDMFRQASQKVRVNENDTSKVVLTMPAGFATPTFITDPEAEI
                     WVNEEFKAKGSWTGRLLAGTYLVEARKEGHRAAIKSINLENGEVRDYDIGSPSVMYGI
                     LDLNTTPPGAEITINGKAHGLTPQMYPDFPVGTYEVRFSRKGYETFTKTVTVTETKAT
                     VISHQLVKAGQSARDLSRGTTQTPTQTSTKTEDARSIETWVETPVEDKLSGQGTELLS
                     GPPKVPKTSKASEAPKTSKAPKKQATTPSSKSSLTWFVTPGIHVSANRIQSLNYPDRS
                     RQFSEVVSCNSLSTGLIFGKLGFYVNYKTDLLTSMASKSVMVKPELYALTGRGKPSFD
                     YVYEGGQLYLSDSWGNPNRDVRLPEGMIVGSSANRFYRRAYTVGAIWKPSELLHLKIG
                     VGYGLDKRIVYGVRTLGTNEEVIRIGIKGPDNGGEAELGAGLDFGLISVSASVSTLNF
                     KYFEAGINVGLSFTFRK"
     gene            complement(12313..13086)
                     /locus_tag="PKY68_01225"
     CDS             complement(12313..13086)
                     /locus_tag="PKY68_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOH23692.1"
                     /translation="MIVLILTGAKAQIHVDEFKRISSGEALTNQKKDHNGQICALIKI
                     NTRNLDDTQRKRLRFQTDAVSQIVSIDYPVGAIWLYISPEAEYLEIAHPDLSVFKYVF
                     KEIIQSKSDYEMTISTAVVETIVRPTITEQYLVITVEPKEALVTLDGELIIPDENGNV
                     TRRVRIGTHECEVSAPMYYSEKQNIIVTIDQRKEVLIRLKPQFGYIYVESLPESEATV
                     LINGRQAGITPLLSDKLEKGEYIQRIGGQGYVSSGQPEG"
     gene            complement(13135..>13565)
                     /locus_tag="PKY68_01230"
     CDS             complement(13135..>13565)
                     /locus_tag="PKY68_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23693.1"
                     /translation="RYDYLESIFADSALIIVGTQLKRTAVSKDMENFVKDNILYEQKS
                     KAEFMESLRRSFKSKNYINLRFEDINMMKKSGQEIYGVQLKQNYYSNNYNDVGYLTLL
                     VDLSEELPVIHVRVWNKNKNEYLSAKTIITRPYGYQLGEW"
BASE COUNT         3430 a         3352 c         3130 g         3653 t
ORIGIN      
        1 agtgagtacc ggaatgccat aacctttttt atcgtaggtt ttatgctcca tttcggctat
       61 ggtgtattgc catttatcca taaacatggt gcaacccgga cagttggtca cgataaaatc
      121 gggctcgtag ggctgcatcg actcaaattt ctttttggaa ttagccaccg aataacccct
      181 gttggctctg accacgtatt gcctgaaacc aaaaccgcaa cagtgacggc gctcgggata
      241 gtctaccacc tcgcctcccc aggattcgat catacccgcc agcacatgcg gaaattctgc
      301 tttacccact cccttgtccg gaaagatttt agcgtaatgg cagcctatgt gttccaccac
      361 ctttaggggt ttgccggtga aacggttgac caggcgatag cgcattttct ctttgagttc
      421 ttcgcgaaac ttatagataa tatccgaagg atgagcgatg tttttgggca actcaaaagt
      481 tctgccggtg gtttcgagca agatgttccg tgctttttcg agcatatccg gctgatgttc
      541 ccagagatcg acgatttcgg aaaaaacgcc gaaagaagta acgcaagagg gcacgtagtt
      601 ctcgtaaccg gcttcggtca taagcgaaaa cagacgagcc acaatggtca tggtggtttc
      661 caaaggcaca atatcggaat agaaacctat gcccgtacag gtgtgctgat tttccacatc
      721 gagaatattc ttgcccagct cttctttgag tattctcaaa aaagccgttt cagatcccgg
      781 aaagaaggtc tgccggatgc aactgcgttc ataaaaatac ttatcgccgg caatctcttt
      841 ttggtattcg ttccaataat gtttttttgt agtcattctt tggaagtatt tctgttatag
      901 accaaattca acaagtcctc gtcgtttctg atgttggttt tgttttttgc tccggtttcc
      961 actttgcgca tcagttcttt gcctccggta gcttcaaaaa tcttgttgat ctcttccagg
     1021 gtttccttgc ggatgttgcg caaagcgccc gggccttcac cgtgataatt ggcgccaaag
     1081 cgtggggcaa tttcctcaat gtacttggta taccacttcc ataccggccc ctgttcggga
     1141 tgctgttcgt agctcatggt atcgggatgt acgcaatagc ctgtatccaa aatcgtttgg
     1201 gctatttttt tggagatggc aaattgttgc aggccttttt cgctttcggt gaaatagccc
     1261 atcttttggg aaacactcct caggatagct atcaatactg cgggagtatt gcctcgcgga
     1321 catcgggttt tacacgagag acactgccca caataccaaa tggtttcgga acgtaacaga
     1381 gcttctattt cgtcctcgtt acgacgttga atggtatcga caattttgcg cggatcgtaa
     1441 ttgtaaaact cagccgccgg gcatacggcg gtgcaaattc cgcaattcat acaggagttc
     1501 aaggcttctt cgaagcggac atcctgagtg agtaaattgt ataaattccc cattagctat
     1561 gttttaatcc gatttattta ccggcaacag ctaacacttc tttgtgaaaa gcagctgcac
     1621 gttgcatgcc ttcgccgcag ttaatcagta cggtatcgcc ctgatggata cgtttttctt
     1681 ccaatgcccg gtaaaaaccc gccagacata caatggaggc cggcccgagt aacacaccct
     1741 gttgatcgta gacctcacgt gccagagcgg gcaagctcgc ttcgctgata cgcaaaaaac
     1801 tgccgccggt tttccgtact ataggagcaa taaccggata cataccggga ttgcctgttg
     1861 acaagatgga cactttggtt tgaggattgt ctataaccgg gtaattcttg tggtaatttt
     1921 cggggaagcc ctctttgacg gcctgctccc aggcctgcac catgggatcg caaaggtctt
     1981 gctgcaccag taacatccgg ggcaaaacga cttcgggata atacttttct atttcgcgta
     2041 cccctttgtc gatcgcaata ggacctgtgc ccccggctac tgcctgaata tacacctcgg
     2101 gcattttact caattggcgc ataaactcaa aaaccatggt ttttttggct tccacccgta
     2161 tgggatcgaa attaccggca gaaatcaaca ctttgttttg acgggcgtag tcggccgctt
     2221 cctgtttggc ccgggcataa tcgccttgcg agcgaaccac ctcctggccg aaacccagaa
     2281 tttcctgcac agagtcttcg ttggcgccgg caggcacaaa tactttgaaa gctacacccg
     2341 ccaaagagag gtatttggcg taagcggtag cggtgttgcc ggtggaggct atgcaatact
     2401 gttttactcc atgttctttg aacaaggagg cagccatgga accggctacg tccttgaagg
     2461 tctgtgtgcc tccattcaaa tcgttgcgat ataccaatgc ccggcattcg ataccgtaat
     2521 gctcgcgggc aaaatcttcg agaaaatccc aggattcaat gggaatagct ccttctttgc
     2581 aggagattat gttgtcgcga ttttcgagcg ggagaaaatc gaaataatgc cagaagctgt
     2641 cggctccctg cttgtataaa gcttccagtt gcgtgtaatc tgcctggtat tctgcctcgg
     2701 aatgtttgct accgcacttc ggacaggtct gatcctgttc aaaccaggca gcaaagccgg
     2761 ggcttatata gccgcattta acacacttga gctgagattt catttttgct tgtttaccag
     2821 gttcatattg ggaatttccc gcttatactc gccggtttcc agttttttct ttatctcgct
     2881 gaacacgttg atggtacgtt ctacgtcttc catgcgatgt gctgccgtcg gaatgatgcg
     2941 tagcataata acatctcttg gtaccacggg atagaccact atcgaacaga atatgttgta
     3001 gctttcacgt aaatctacca caatattgga accctcgttg acaccgccct tcaggaagac
     3061 aggtgttacc ggcgattggg tgcgcccgat atcaaaacct ttttccttga gcccgttttg
     3121 cagagcacga actataatcc aaagattttc gcgcaattcc tttttggttt tgagcaattc
     3181 cagcctcttc atagcgccga ttaccatggg catgggcaat gatttggcaa aaatctggga
     3241 acgcatgttg aaacgaagat gatcgacaat agcccggtcg gaagccacaa aagcgccaat
     3301 accggccata cttttggcaa aagtggagaa ataaatgtcg ataccgtcct ggcaattcaa
     3361 atgttcgccg gtgccggccc ccgtcgcgcc catggtaccg aaaccgtgag catcatctac
     3421 cagtaaacgg aacttgaaat cctttttcat cgccacaatc tcgtcgagtt tgcccaggtc
     3481 gccggccata ccaaatacgc cctcggtgat gaccagaatt cctccccttt gctgttcggc
     3541 aagggctgta gccgactcca tttgggcgcg gaagctatcc atgtcgttgt gttggaacac
     3601 aaaacgttta ccaaaatgca tcctcaatcc gtcgatgata caggcatgcg attccgagtc
     3661 gtaaacaacg acgtcgcgac ggctcagcaa ggtgtcgata atggacacca taccctggta
     3721 accgaaatta agcaaatatc cggcttcctt gcctacgaaa tcggccaggt cttgttccag
     3781 tttcttgtgg tgcacagtgt cgcccgacat catacgggca cccatgggat aagccagacc
     3841 ccattccttt gaggcatcgg catcggcctt acgtacctcg ggatggttag ccaaacccag
     3901 gtaattgttt aagctccaca ccagcacgtc tctaccattg aaacgcatta tgggttggat
     3961 ttcaccttcg agtttcggga acataaaata gccgtcggca gcgccctgat attggcccag
     4021 cggacccgga gttgcttgaa ttctttcaaa aatgtccttc attactagtc ttttaaaaga
     4081 ttgtgtgata taaaaaataa aaattgtttt ggttttgaat taaatttcct ctcattatca
     4141 ataagttatc agtcctatac aaaaaaggca tattattcgc aagggcacac ttatcaactg
     4201 acaaaggtaa cactacgagc catactcttc taattcttaa caatatttaa ttggctgcga
     4261 ggaccgggac cgggcttttc atatctgttc gtttatcaac aaaatcgtgt acccgacgta
     4321 catcaaaagc agaataccgg cttcccagcg gtcgagccgt cttttcttgc ccgaaaacat
     4381 ggcaataaaa accagaatgg ttccaaagct caaaaaatac atttcgaaat taaacttggt
     4441 attgtactcg ataggcctga tcaaagagct gacacccata ataaagaaaa tattgaaaat
     4501 attggatccg atgatgttgc ccactgcgat atcgttgttt ttcttaatag cagccactac
     4561 cgaagtagcc agctcaggca gcgaagttcc gaccgccact atggtcaatc ctataatggt
     4621 ctggctaatg ccgaggaact cggcaatttt caccgcattg tttacaacca acctgcctcc
     4681 tactaccagc aaggccaatc ccaaaacgat aaacacgatt attttgaaga tagtgaattc
     4741 tttatgttgt tcctctacag aaaccgtgtt ctcttctttc aattgcctga atacatagta
     4801 gagaaaaaag gcaaacacca gcaaaagaat aataccatcc cacctggaca acaattcgtt
     4861 ttgggtaaaa atactattgg aaagaagata gagtataaca actgccagta aggataaagg
     4921 tatttctttc caaaccgtac tggcttgaac tatcagggga gaaatcaatc cggcaatacc
     4981 aagtatgccc aaaagattga aaatattact ccccagaata ttgccgtaaa cgatgtcggc
     5041 atgccctttg taagcagcaa atacgttgac caccatttcg ggcatggaag taccgaaagc
     5101 aatgatagta agacctatgg ccaattcaga aacatggtat ttgcgagcca gtgccgcacc
     5161 tccgtcaacc aaccagtcgg ctcctttgat caacaaaacg aatccgagca gcaacaacaa
     5221 aatttgtatt gtcataatta ccaaaataaa aacacgttca ttatttctgt ttcaacagac
     5281 ccatttcgta gagccagttt tcacataagg atgtccaata attgagcatg gccgattcgt
     5341 ttttcaacga aatggaatgc cggccatagg ggaaaatatg taaactaccg tcaacaccct
     5401 tttcgagcat ggcgttgtag aattgaatac tgttttgtac aagcacggta ttatcgttgc
     5461 gggcgtgaac aagaaaggtg ggcggcgttt gttccgttac ctgtttttcg ttggaataat
     5521 aatcgcgctt ttccttgccg gcatcggctc ccagaaaagc ctgcacactc ccttcgtggg
     5581 caaaatcgcc catattgata acagccgata tcaacaccat aaaatcgggc cgaacagaat
     5641 agcgatcgat ggaatctccg ataagcgaaa agtcttcgaa atgcgttccg agggtcgatg
     5701 ccagatgccc acccgccgag gccccgacta ttcccacttt gccaggatcg atattccgga
     5761 cagaggccat atttcgaatg attttaagcg ctctttgggc atcctggata ggtccttttt
     5821 ctctttcgat caggtcggga gaattcggca ggcgataaac cagcacaaaa gcatttacac
     5881 ccatggtatt gaaccattta gcccattgaa aaccggccag atggtaggtc agtttttgat
     5941 agcctcccgg gggacaaatc aaaacggcac tgcctttatt gtcttcgttt gatgtccaga
     6001 aagcatacat gcccggagtt ttcacctgtg taattcgctg cctctcctcc ttgtgttcca
     6061 gggtcaaccc tttggaattc ggcattttgc cttccggcca aagcggtatg aattcctggg
     6121 caggggcatt aaaaaaaaga cataagcccg gcaaaaagaa cagcaaaaat ttaatccgta
     6181 gttggctcat tttttctgca atatgatctg ttgattattc tcattgatta ttcccaggcc
     6241 aaagtgcatt ttcccaccgc ataaaaggct tcctcccgca gatccatcaa tccggtatag
     6301 attacggtaa atgtgccgtc gtcctcttcg atggcacaga gcggggtgcg taaagaatgg
     6361 tttccatctt tgacccaggc ccggtcttcg aactgcactt tgattctggt ttctttgctc
     6421 cagttcagac cgtcttcgga tagactgtag ccgatttcac gatcaccaaa agaatcaaac
     6481 acagccaggt attttccgtt tttcaactga gtaataacgg tattttccat aaactcgtcc
     6541 acgatgggca agggattgaa atcttcggtc atcctcgtcc agggaccttc cagcttttcc
     6601 gcccgtgcca ttcccgtcgg ccaacctccc tgaggaatgt aattgtgccc gtcgtaaaaa
     6661 gcataccagg tatctcctac tttgtacgga ttgaagctgg caacagcctg ttgtccttcc
     6721 caggcctgag aaagttcgcc gggttgcaag actataccca tatcggcgta ggggcctgca
     6781 atgcccttaa ttccggggat gacagacttg gcacgccaga ttctgccact gtagtccgac
     6841 cgggctattt ctccctttgt ttcgtctccg gccctatagg ccacataaaa aatattccag
     6901 gcgttttcgt cttcgttgaa ttcaactccg gtaacccaga cctcagaacg cggattgtag
     6961 ggggatctgc cgggaatact ttcccttatg gtcgcccggc gctcccaatt cagcgcatcc
     7021 ttgcttgtcc agtaagaaat acgcagatcg cgaaaaggac gcacaaacat ttcgtttaca
     7081 aacatatggt aagtaccgtc cattttaata acctgaccgg tctcaaaacc ggaaaaattt
     7141 ctgctcagaa tcacatcagg atgctcagga ccaatcacct cccggggatg agcagacaga
     7201 agtttgagtt tagcgggagg attggtgcga ttggtataac cagtgtaatc tgcacgattt
     7261 gcctgagcag aggctgccaa atcgaaaccg gcaaagagaa ccaacaataa tgctctcaaa
     7321 aagaataact tagaatgttt cactgaaaaa gcgcttgaaa aagacgggca aaggtacaaa
     7381 ataatttaag ccgtatgcta tttccgtcgg aagtaatgtt gcccttagcg ctgtgattgc
     7441 ggtgatcaat acaagcggat cggttgcaaa gcttgaaatg aatagtgata attagtcgct
     7501 cttaacacaa cgaacagaaa accccaactc cttaagacca ttgtcgtaag cgtacacagc
     7561 actgtcatcg aagtacacat accggtacca gacgccacca ccgccctctg tggcagacca
     7621 ccagaaaccg ccgtagccaa ggtattgaaa tactccataa ttgttacgta agccacccgg
     7681 gaggcctgta aaaccggtct cgttggtgga tccgggattt gaacctgccc aatgcccggt
     7741 tccgctttct ttgagtttat taccggcaat gctttcaccg cccaggtaat cggttaactc
     7801 tgtccattcc gcatggcttg gaacatgcca gccttgtgga cataatttgc ccgtttctac
     7861 cgtaaaccaa ttgtatagaa ggccataatc gctgtataag gccgggtcat tgttgtaccg
     7921 gcagtagccg ggtgttgtca gggtgttcca gcttgaattg tcggttatat gaggtatttc
     7981 tgtcccatcg ttcagaaggg tgcttttcag gttttcggcc atccaaatct gattgcctat
     8041 ggctactgtt ttgtaaacat tgccttgaat atccaggacg gtattgcctg gaggattggg
     8101 gttgtcgtcg ctgtcaaact caataaccga agtcggggta atggaagacc gcactatcct
     8161 gagattttgg gtagtttcgg tcatttgttg atgatcggtg gtacgcacga tcaaggtcac
     8221 tgattcatag agggccggag gaagtacaaa ataaaaggct gtggcttgct catcatcgag
     8281 ttggacacct tcgccgcaat cgagggtaag ggtgtcgtaa gcaggcggtg taggcattat
     8341 taattggggc accccctggt agcgcatatc gactccggcc accccggcca gtccgggagc
     8401 acaaaggctt agagaactta cggacacgtc tccacataga ttcagcttga gcaggccgca
     8461 aagattttta aaatgcaagt tggtatcgta actccaggaa accatggggt aagctccact
     8521 ggcaaaaccg ccggccttat attgttgcgt atggggtaat ttcagtgtca gcaaagtatc
     8581 tctgttcagg ttgagggcaa gtttccaggg ataaaaggca aaatacatat cggcatcgat
     8641 gagatcaccg ctaaaagtag cgtaaggcaa ggtgtcgctg cttgtgttga caaaaggctt
     8701 aaactcaccc aaatcggcgg aaaaagcaat gctgtcgagg ggaagaaagc aaatgctgcg
     8761 cacagggtcg ccgggttctc cgctaagcag ggttttggtc tgagcgtttt ccgtcgaggc
     8821 atgaatgctc attggggaat tttgatgctt gccgtcccgg gaagaaaaca catcaccggt
     8881 acatcccatc agcaacaata aaaccggaaa acaaggaata aacctatgtg gaatggtctt
     8941 catacttatt atctaagatt attattgtct aagatttacc agtcgtgttc atcctgagta
     9001 tccaggtctt cgccttcgtc gtcgtctttg atacagcgaa cggaaaatcc gtaacctttg
     9061 ccgacaatat acttatggaa aaaactggcg ttgtctttgt ccatatagac caagcgagcc
     9121 gtaggcgact catattcatc cgatgtccaa aaataagccc cgaaacccaa gctggaaaag
     9181 tttccgtcac tcatacgata tcccgcaggt aaggcagaaa aataagagtc gttggtggct
     9241 ccggtattgg gttctgccca atggctggta cccgcctcct tgagccggcc tccggccagg
     9301 gcttctccgc caaggtaatc catcaggctg acccaatcgt cgtgcgaagg tatctcccag
     9361 ccttcgggac aaagtttacg atcatcgtca acggcgtgcc agttgtagag ctttccgtaa
     9421 acggacacat agccggcatc gttttccacg ttacaagcgg cgggcagagt ttgcgaatca
     9481 tatgtgctcc attctgcctg atcgctgatt tccggaatag cttctccagt tacaaaacgg
     9541 gtcgttctaa ggttttcgac catccaggta cgatcgccta taatcacggt tttataactg
     9601 ttgccatcga tgtcgctgat gctgccatac tggggagccg gtgtggtaaa actcagttca
     9661 tccccgtaaa cagtgccgta ggcattggtg gcgtaagccc tgatgtaata agtggtattg
     9721 ggttgcagac cggtcaagtt actggtatat tcccctgttc cggtaccgtc ttcggttttg
     9781 ttatcgcccg tggtgggata gccgtagttg ttccagcaaa caccccgtgc agtaacggca
     9841 gaaccaccat cggaagtaat gcggccaccg cttttagccg aaatgtaggt aatggagtac
     9901 gctgcaacgg tactgatttg aatcgaagta gactcgtctt tgatgcagcg taccgagtat
     9961 cctccaccct ttttgcctac aaaattgccc ctgaaccagg tgctgtcgtg gtctatatac
    10021 cgcatacgag cccactcatc atcccaaccg ttggacgacc aaaaataggc cctgtaacca
    10081 agattgagaa aaacaccttc ttcctggtta cgaaaaccgg caggcagagc cgaaaaacga
    10141 atatcgtctg tggctcccac attaggtgca atccaatgcg cggtaccggt ttctttgagt
    10201 tttcccccgg ccacagactc accgcccagt aaatcggcca atgctgccca atcctcgtga
    10261 gtgggcactt tccaaccatt tgggcagagt tttcggccgt cgtttaccgc ataccagtta
    10321 taaagccttc cgtattcgta atcgtttgag ctgctgttat taacgctgca tgaagccggc
    10381 cagccggcca aggcgtaaga ggtccaatcg gtattgtctg ccagataact tatctcatcc
    10441 ccattgctaa acctggtggt tttaaggttt tcgaccatcc agatttgagc gcccacttcc
    10501 atggtacgat aggtgttgtt gtcgatatcg gtcagcgttc cgaattcttc tgcctcggtg
    10561 ttaaaaacct gctcgttgcc ataacccgtt ccgttagcat tggtggcata agccctcaca
    10621 tagactttgg tatggggtgg cagtccggtc atcgtactgg taaattctcc ggttccgcct
    10681 ccttcttcgg ttttgtagac ggtgtttatg gtgggattgc ggctgtagtc ccagcaaatg
    10741 cccctctcaa taacgggaga accgccgtcg gacaaaatag taccccccga tactgccgag
    10801 gtagccgtta tgttgctcgg cacatgagtg ctcaaggtcg gcagggtcga ttgccacatt
    10861 acgccgtcga aattctcgta ctccagacag gccgccatcg aaacgagcag caagatattc
    10921 aggatatgtt ttacaaaact tctcatcgat tattttctaa aagtaaaact cagccctaca
    10981 ttgatacctg cttcgaagta tttaaagttc aaggtactta ccgatgcgct gacactgata
    11041 aggccgaaat cgagccctgc acccaactcg gcctcccctc cgttgtcggg tcctttgatt
    11101 ccgattcgga tcacttcttc attagtaccc agcgtcctga caccatatac tatccgttta
    11161 tcaagaccgt aaccgacacc tatctttaaa tgcaggagct ctgacggctt ccatatggca
    11221 cctacggtat acgcacgccg gtaaaatctg ttcgcggaac tgcctacaat catcccttcg
    11281 ggcaagcgga catcgcggtt gggattgccc caactatctg acaaatacaa ctgtcctccc
    11341 tcgtaaacgt aatcgaaaga aggttttcct ctgccggtaa gcgcataaag ttccggctta
    11401 accatgaccg actttgaagc catggacgtg aggaggtcgg ttttgtaatt cacataaaat
    11461 cccagcttac cgaagataag tccggtactc agggagttgc aggaaaccac ctccgaaaac
    11521 tgacgcgatc tgtccggata attcaggctt tgaatacggt tggccgaaac atgtataccg
    11581 ggagtaacaa accaggttag cgaagatttg gaagagggag ttgttgcctg ttttttagga
    11641 gctttggaag ttttcggagc ttcggacgct ttagatgttt tgggcacctt gggcgggccg
    11701 gacaaaagct ccgtgccttg tccgctgagt ttatcctcga ccggcgtttc gacccaagtc
    11761 tcaatgcttc gggcatcctc cgtctttgta gaggtttgtg tcggagtttg ggtggtgccg
    11821 cgagacaaat cccgagccga ctgtccagcc ttgaccaact gatgagaaat cacggtcgcc
    11881 ttggtttcgg ttacggtcac cgtttttgta aaagtctcgt agcccttcct gctgaaacga
    11941 acttcatagg tgcccaccgg aaaatcggga tacatctgcg gagtcagtcc gtgggcttta
    12001 ccattgatgg taatctcggc tcccggcggc gtggtattca gatcgagtat gccatacatt
    12061 acggagggag aacctatatc gtaatcgcga acttcgccgt tttcgagatt gatagacttg
    12121 atggccgcgc gatgtccttc cttgcgggct tctaccaggt aagtgccagc cagcagtcgg
    12181 cctgtccagc tgccttttgc tttgaattct tcgttgaccc atatttcggc ctcggggtcg
    12241 gtgataaaag taggggtggc aaaaccggca ggcatggtca acaccacctt ggaagtgtcg
    12301 ttttcattta ccctaacctt ctggctggcc tgacgaaaca tatccttgac caccaatacg
    12361 ttgtatgtat tctccttttt ccagtttgtc gctcaacagg ggagtgattc cggcttgacg
    12421 gccgtttatc aaaacggtag cttccgattc gggcaaggat tctacgtaga tatagccaaa
    12481 ttggggtttt aagcggatta aaacttcttt gcgctgatcg atagtgacaa taatattttg
    12541 tttctcacta taatacatag gcgcacttac ctcacattcg tgggttccga tgcggacccg
    12601 gcgggtaacg ttgccgttct cgtcgggtat gatcaattcg ccgtccaaag tgactaaggc
    12661 ctctttcggc tccacggtaa tcaccagata ctgttcggta atggtgggtc tgacaatggt
    12721 ttccaccacc gcggtgctga tggtcatttc gtaatcgctc ttgctctgaa tgatttcctt
    12781 gaagacgtat ttgaataccg aaagatcggg atgagcaatt tcgaggtatt cggcttcggg
    12841 cgaaatatac agccagatag cccccaccgg gtaatcgatg ctgacaatct gcgatacggc
    12901 atcggtctga aagcgcagtc ttttccgctg tgtatcgtcg agattgcggg tattgatttt
    12961 gatcagggca caaatctgtc cgttgtggtc tttcttttga ttggtcaaag cttcgccact
    13021 ggaaattctt ttgaattcat ccacatgtat ttgtgctttg gctcccgtaa gaatcaaaac
    13081 aatcaaacac cctattaaac aacatttctt catttacaaa ctttaagttc gtttttacca
    13141 ttctcccaac tgataaccgt aaggcctggt tatgatggtc ttggcagata aatactcgtt
    13201 tttatttttg ttccacaccc tgacgtgaat caccggcagt tcttccgaca aatctaccag
    13261 cagagtcagg tagcccacgt cgttgtagtt gttggaatag taattttgtt tgagctgtac
    13321 gccgtaaatt tcctgacccg attttttcat catattgatg tcttcgaagc gcaggttgat
    13381 gtaatttttg cttttgaaac tacgccggag actttccata aactccgctt tgcttttttg
    13441 ctcgtaaaga atattgtctt tgacaaaatt ctccatgtcc ttcgataccg ctgttctttt
    13501 taattgggta cccacaatga tcagggccga atcggcaaag atgctttcca ggtaatcgta
    13561 acgtt
//
LOCUS       DAOSGL010000017        13472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_3808_length_13472_cov_4.146009, whole genome shotgun sequence.
ACCESSION   DAOSGL010000017 DAOSGL010000000
VERSION     DAOSGL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3808_length_13472_cov_4.146009"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1149
                     /gene="lpxB"
                     /locus_tag="PKY68_01235"
     CDS             16..1149
                     /gene="lpxB"
                     /locus_tag="PKY68_01235"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOH23694.1"
                     /translation="MKYYLIAGEASGDMHAANLMAALVEKDPKAEFRFLGGDKMAAIG
                     GTPLIHYKKMAYMGILPVILHARTLLRNMRICKKDILSWQPDVLILIDYPGFNLNIAA
                     YIKKKQPGLPIHYYISPKLWAWKGYRIRNIRRDIDRLYCILPFEKAYFDRKNYRAFYV
                     GNPSLDSIEAFKNSKPDLKGWREKQQLTQKPIIALLAGSRRQEIKANLPLMLRVASAY
                     PDHQLVLAGAPGINKDFYAPYIKNYDCRLVYDETYSLLAQSKAALVVSGTATLETALF
                     GVPQVVCYALWIGGFLYKIGRKYLIRTPFISLVNLIAEKEVVVELVANEYNYEQLKNE
                     LNKILYDSAYIAEMQEAYGFMRKKLGEPGAADRTASLIYNHLT"
     gene            1249..2013
                     /locus_tag="PKY68_01240"
     CDS             1249..2013
                     /locus_tag="PKY68_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH23695.1"
                     /translation="MGKIIALANQKGGVGKTTTAINLAASLAVQEKKVLLVDADPQAN
                     ASSGLGIDIRQVGISIYECMIDGANPADGIQKTAIEYLDILPSHIDLVGAELEMINIE
                     NREKILSAVLQPLKSSYDYILIDCSPSLGLITVNCLTAADSVLIPVQCEYFALEGISK
                     LLNTIKIIKSRLNTELEIEGFLLTMYDQRLRSARQVADEVRRHFQGLVFKTVIQRNVT
                     LSVAPSHGQPVMLYDADSRGAIDHIQLALEIIRKNK"
     gene            2026..2898
                     /locus_tag="PKY68_01245"
     CDS             2026..2898
                     /locus_tag="PKY68_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOH23696.1"
                     /translation="MAAKNRLGRGLDALISTEDIQTVGSSSISEVELELIRPNPEQPR
                     TDFEPEALEELAISIREIGIIQPITLRKMDDGYYQIISGERRVRASKSIGLTKIPAYI
                     KTAEDDSMMEMALIENIQREDLNAIEIALAYQKLAEVYKLTQEKLSERIGKKRTTISN
                     YIRLLKLPAEIQMGLKNKTIDMGHARALINVKSPGLQLALYEQILKQGLSVRKVEELV
                     RDEESGKSKNFKKPLPRKTSEEYLDLQKQLSQKLGTKVRFSINEKGKGSISIPFDSEK
                     KLEEILLSLDKLFN"
     gene            3011..3697
                     /locus_tag="PKY68_01250"
     CDS             3011..3697
                     /locus_tag="PKY68_01250"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOH23697.1"
                     /translation="MADSLSSVLADDPSLNGVNGFLTADSLSPMLNDDLSRNGADGFL
                     ISDSLFLRPDMESFMNTDTAARERQLFKPLPSKALWYAALCPGLGQFYNRKYWKLPIV
                     ASGVVGLAYAISWNGKYYVAYTNAYRDISDNNPDTNSFLDLLPPGYSYTESQLRTVLK
                     NRQQTFRRQRDLSIIGAVGFYLICILDAYVDAELFDFNVSPDLSIRLQPVRQAPGIDH
                     AMELSCLFRF"
     gene            3719..5359
                     /locus_tag="PKY68_01255"
     CDS             3719..5359
                     /locus_tag="PKY68_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOH23698.1"
                     /translation="MKRIIYILLILGLSASPALSASPESSASPASPESSVSPESSVSP
                     ESSVSPASPASPESSTSPASPASGQFFKKIFTKKASTEQTEGLPGEAGNLPQQKQVNG
                     KQTGKKLSHSLSNPEKTGQNNLLAEEDAPAVEMNLDSIENSLFSEGLKILIEMDSSHY
                     FDDPGLDPSFHFNANTDSLIYEWQRQSLLIFDENCRPTLTDSSATPETTYIQRLMDLP
                     NIIEMPYNRIVRRFIDLYLNERRMQVEAMLGAGVYYFPLFEQVLDQNGLPIELKYLPV
                     IESALNPKAVSRVGATGLWQFMYATGKMYGLQINSLTDERRDPVKSTYAAVKYLKSLY
                     SMFGDWNLAIAAYNCGPGNVNKAIHRSGGKRNFWEIYYYLPRETRSYVPLFIAANYVM
                     TYYSEHGICPAQNNFPPVIDTFMIHQNMHFQQIAEVCQIPMQEIQLLNPHFRNEIAHG
                     TASKAGILYLPMEYSPVFTELQDSIPRYKEQELLASRLVSVQSGQAGPTDAIVYRVKR
                     GDNLSVIASRHNVSVQKLKNWNNLKSNTLQIGQRLLIY"
     gene            5405..7699
                     /locus_tag="PKY68_01260"
     CDS             5405..7699
                     /locus_tag="PKY68_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOH23699.1"
                     /translation="MDILQATNPEEEKIFEQFQLLLNDYLNSNHRKKVDLITKAFKFA
                     YNAHRGVKRRSGEPYIMHPLAVARICCAEIGLGSTSICSALLHDVIEDTEFTVEDIRN
                     NFNDRIASIVEGLTKISGGIFGENASTQTENFRRLLLTMSEDIRVILIKMADRLHNMR
                     TLSALSPSKQYKIASETMYLYAPLAHRLGLNRIKTELEELSFKYEHPEAYEQITHKLA
                     ITAESRKALYYSFIEPVQHELDKLDLKYYIKERVKTAYSIWKKMTTKNIRFEEIYDLL
                     AVRIIFDSPDIEDEKEMLALEKKQCWDIYSTITNIYKPHPDRLRDWVNNPKANGYQAL
                     HTTVMGFTGDWIEVQIRSTRMHSIAERGFAAHWKYKLGEIEEDNELNHWILTIKEILE
                     SPEPNTLDVLDTIKMNLFSSEIFVFTPKGEIKTLAQGATALDFAFELHSELGYRCIGA
                     KVNHQLVPMSHVLKSGDQVEILTSENQTPRYEWLKFVTTGKAHSKIKARFRKDNRETI
                     KKGEELLNEFFNKHHLTADADNVQVLLTNCNCINKEELLMKLGTGELTLNNAEAELFK
                     KDNQNLLVKYWKLTFGSKNSKQEKEKGKEKEKENKTIDFKSTHRLTEESVQNEYHLSD
                     CCQPIPGDAVLGYVAQNGIIHVHKRQCPIAMRLKSSYGDRIVSAEWGTHKKLSFAVEI
                     EITGLDTVGMINQITRIVSDEFSVNINKLNFESKDGVFRGSMTVYVHDVEGLNHLCNK
                     ISKLKNIKSVTRVERQNKLMGVKE"
     gene            8017..8673
                     /gene="cas6"
                     /locus_tag="PKY68_01265"
     CDS             8017..8673
                     /gene="cas6"
                     /locus_tag="PKY68_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOH23700.1"
                     /translation="MRIHLKFKISNQTVPFDHQPLLVGAIHKWLGQNEEHGEISLYSF
                     SRLEGGKATNEGIKFNQDGLFFFSSINIDLVKRLISGIRSDPSMFFGLRVNELFIEED
                     PDLSNRELFFPGSPIFIKRRNGERIEQIFFDDPRSSEYLRETLLTKMGKVGLEDDSLD
                     IRFDLSHTKSGTKMITYKGIKNKANWCPVIIKGKPETKLFAWNVGLGNSTGIGFGAIK
                     "
     gene            8683..9273
                     /locus_tag="PKY68_01270"
     CDS             8683..9273
                     /locus_tag="PKY68_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23701.1"
                     /translation="MYYVVKYSGPFGFIKPWTAVRDSETYSQQFLTPSIIEGIEKKLF
                     PELLHKTGKILKIVRYRLNYCGIDMQQERTRSKGEFEKEKKTDSLGRTYYTAKNMGVI
                     TRGVLLQPNLYLAFKDEQDANNAFAQTICLCRNEDLLFPEILTEMSEEDFDKIDGFEL
                     ETSSPESGFKVGYNRFDDFAEMFGELKVVGNPIRDR"
     gene            9273..11438
                     /gene="cas3"
                     /locus_tag="PKY68_01275"
     CDS             9273..11438
                     /gene="cas3"
                     /locus_tag="PKY68_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOH23702.1"
                     /translation="MKPLDQILAKSVNYGNISLLEHTQEVTLAIENFARHFDFTFNSE
                     LARKGAILHDLGKAHDHFQKKIQNINSKSLAEAREENYIHRHELSSLAFLPVFPKEEW
                     DYLIDMIVAHHKSIENDPRGRGILDLDQNSQYWINNHLKNWEEWSQYGLCILNIFGYK
                     SNIITLDEARNALLYTVEYCESKGNGWSPWRGLLISADHFASAFTNKTAENLQHLFET
                     PDLSYYHDLSRRNHLYPLSEISTEDSRLHTLVVAPTGAGKTDFLLKRCKGRIFYTLPF
                     QASINAMWERMKNTIPNKDIRLLHAMSKIIVGKNIEERILQPLVGSTVKVLTPYQLAA
                     IVFGTSGFEGVMLDVKGCDIILDEIHTYSDYSKSMVLEIVKTLLRLDCRIHIGTATMP
                     SVLYNEVLSILGGKDKVYEVKLPGDVLKSFNRHKIFKINSDDEIPNILDQAFRNREKV
                     LVVFNTVKQAQDAFKTFSERFTKIPMMLIHSRFRRGDRVELETRLKRQFNGDGSKEFG
                     DGLCPCLVVSTQVVEVSLDISFDRIITQCAPLDSLIQRFGRVNRKRSKDTIGKYKPVH
                     IIAPHDKVLPYKMDVLKRSFEQLPDDGTILNEQNLQEKIDNVYPVIETKEIDVHLIYR
                     NNRYSLRELTNTKKAVLVEALEIESATCILETDREKYLSADWEDRIYMEIPINWKTMD
                     RYKSEYEQLDVGANPFVVPQNLNDYRIFGLRLVENDKFL"
     gene            11441..12760
                     /locus_tag="PKY68_01280"
     CDS             11441..12760
                     /locus_tag="PKY68_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23703.1"
                     /translation="MYTSYIGKKFLKLYNEREGTNLSAEEFFEKVFFPLFFDDDRHLL
                     HVGNSPFFQKLKEEDIKKYGSKSLAQYNNLKKAIKNDIPNMSIFVGSAAKDVKGTTSG
                     QVSNINVIVDKEEMYASWMGEALGIRISGGMIMLIDEPDVLWALYSGWRHYRNYLNQT
                     PNVEDKQIETWNGQWLWHWSQPHFDNNNPLDRLHIEVSEVQGNVAISTQQWIKGIFAL
                     SRKFPNKVITAYAYNLSQTNITLGFINLYLPEVNRLFKLRNKIFIDEPRTILTEEEIN
                     SFTTFYNFKCACKFGTIGLKAIEPAKLREYMPIGSVSFAKGKEYKFSNEESYTNYKLF
                     KIWIIAMLNKTELLKLASDVAKALSELDKADDRGKKVFATLSQEVRESNNLRLFIDKL
                     TTVLNQAPSNAETFKEVVEAVLKMPLDNFPLFVTLIRFEYAYLKSKN"
     gene            12778..>13472
                     /locus_tag="PKY68_01285"
     CDS             12778..>13472
                     /locus_tag="PKY68_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas7"
                     /protein_id="HOH23704.1"
                     /translation="MSCSPFIYLRGLRHAEHTVFGVNDGQKFYYDPQFGKKMAYSSGQ
                     QVKRSIIEALNMPFAAITFNWEIDRKENKASQKEPHSPCDPSYPDQLLGGYMKAESGN
                     MTVKRRSPLSISAMRPLHPLLGGIENPLEDLTFDRTSHPDHHQVKVFWLDNKKRGAEL
                     TSDELEEWLTTNRRSLPNRAFIQNQSRASGLFVYDIAIDLRTLFCVSTNKLEPELFPE
                     IETKLRNEGWIEG"
BASE COUNT         4273 a         2993 c         2824 g         3382 t
ORIGIN      
        1 tgagcccttg aactgatgaa gtactatcta attgccggtg aagcttcggg cgacatgcac
       61 gccgccaacc tgatggcagc cttagtcgaa aaagacccca aggccgaatt tcgctttttg
      121 ggaggagata aaatggccgc cataggagga acgcccttga ttcattacaa aaagatggcc
      181 tatatgggca tcctgccggt aatacttcat gcacggactc tgctccgcaa tatgagaatc
      241 tgcaaaaaag acatcctgag ctggcaaccc gatgtactca tactgatcga ttatccgggc
      301 ttcaacctga atattgccgc ttacatcaaa aaaaagcaac ccggtctccc catacattat
      361 tatatatcgc ccaaactctg ggcctggaaa ggctaccgca tacgcaacat ccgccgcgac
      421 atcgaccgac tgtattgcat actccctttc gaaaaagcct atttcgaccg gaaaaattac
      481 cgggcctttt atgtgggtaa tcccagtctg gacagtatag aagccttcaa aaacagcaag
      541 cccgatttaa agggctggcg cgaaaagcag caactgacac aaaaacccat cattgctctg
      601 ctggcaggca gccggcgaca ggaaatcaaa gccaacctgc ccctgatgct cagggtggcc
      661 tccgcctatc ccgatcatca acttgtactg gccggagctc cgggcataaa caaagatttt
      721 tacgctccct acatcaaaaa ctacgactgc cggctggtct acgacgaaac ttattctttg
      781 ctcgcccaaa gcaaggcagc cctggtagtt tcgggcacgg ccaccctgga aacagccttg
      841 ttcggagtgc cgcaagtggt ttgctacgcc ctgtggattg gaggtttttt gtacaaaata
      901 ggacgtaaat acctgattcg cactcccttt atttcactgg tcaacctgat cgccgaaaaa
      961 gaggtggtgg tggaattggt ggccaacgaa tacaactacg agcagctcaa aaacgaattg
     1021 aataaaattt tgtacgattc ggcctatatc gcggaaatgc aagaggctta cggctttatg
     1081 cgtaaaaaac tgggtgaacc gggagctgcc gaccgaacag caagcctgat ttacaaccac
     1141 ttgacctaaa gtaagctaaa aggaatcgac ctagattttg tacaaagaaa aagacagaca
     1201 caattatttt ttctatcttt gtaacttgaa aaaaatctgc gttggtttat ggggaaaatt
     1261 atagcattag ccaatcaaaa aggtggtgta ggtaagacca ccactgctat caacctggcg
     1321 gcctcgctgg ctgtacagga aaagaaggtc ttgctggtcg atgcagaccc gcaggccaac
     1381 gcttcgtcgg gactgggcat agacatcagg caagtcggca tcagcattta cgaatgcatg
     1441 atcgacggag caaatcctgc cgatggcata caaaaaaccg ccattgagta cctggatatc
     1501 ttgccctctc acatcgacct ggtaggagct gaactggaaa tgatcaacat cgaaaaccgg
     1561 gagaagattc tgtctgctgt gttgcagcct ctaaaaagtt cctacgatta tattctgatt
     1621 gactgttctc cctctctggg actcatcacc gtcaactgcc tgacggctgc cgactccgtg
     1681 ttgattcccg tacaatgcga atacttcgcc ttagagggta ttagcaagtt gctcaacacc
     1741 atcaagatca tcaaaagccg gctaaatacc gaactggaaa ttgaaggctt tttgctgacc
     1801 atgtacgacc aacgcctgcg ttcggccagg caagtagccg atgaggtccg ccgacatttt
     1861 caaggcctgg tattcaaaac agtcattcaa cgcaacgtta ctctgagtgt ggccccgagc
     1921 cacggacagc cggtgatgct ctacgatgcc gactccagag gagccatcga ccacattcaa
     1981 ctggctctgg aaattattcg caaaaacaaa taagcccctt acgctatggc agcaaaaaac
     2041 agactgggaa gaggactgga tgccctgatc agcaccgaag acatacaaac cgtaggctca
     2101 tcgtccatca gcgaggttga gctggaactt atcaggccca atcccgaaca acccagaacc
     2161 gattttgagc ccgaagccct cgaggaactg gccatttcca tccgggaaat cggtattatc
     2221 caacccatca ccctgcgcaa aatggacgat ggctattacc agatcatctc gggagaacgc
     2281 cgcgtacgcg cttccaaatc cataggactt acaaaaatcc cggcctacat caaaaccgcc
     2341 gaagacgact cgatgatgga aatggccctg atcgaaaata tacagcgcga agacctcaat
     2401 gccatcgaaa tcgctttggc ttaccaaaaa ttggccgaag tctacaagct gacccaggaa
     2461 aaacttagtg agcgcatagg caaaaaaaga accaccatca gcaattacat acgtttgctc
     2521 aagttgccgg ccgaaattca gatggggctc aaaaacaaaa ccatcgatat gggacatgcc
     2581 cgggcactga tcaatgtaaa aagccccggt ctgcaactgg ccctctacga acaaattttg
     2641 aaacaaggtt tgtcggtgcg caaggtcgaa gaattggttc gcgacgaaga aagcggcaaa
     2701 tcgaaaaact tcaaaaagcc ccttcctcgc aagacttccg aagaatacct ggacttacaa
     2761 aaacagcttt cacagaaact gggcaccaaa gtgcgcttca gtataaacga aaagggcaaa
     2821 ggcagcatca gcattccctt tgactcggaa aaaaagttgg aagaaatact gctcagtctc
     2881 gacaaattgt ttaactaaac aagacctgaa ccggatcgat aagaagatgg gctatttcgc
     2941 caaaatcata ccaatactgc ttttttgctg cctggcctgc cggtcggcaa cccaagacag
     3001 cacggtcctc gtggcggaca gtctttcctc ggtactcgct gacgatccct ccctgaacgg
     3061 agtgaacgga ttccttactg cagacagcct ttcgccgatg ctcaacgacg atctctctcg
     3121 caacggagca gacggcttcc ttatttcgga cagcctcttt ttgaggccgg acatggaaag
     3181 ctttatgaat accgatactg ccgcccggga acggcaatta ttcaaacccc ttccctccaa
     3241 agccctgtgg tacgcagccc tatgtccggg tttgggacag ttttacaacc ggaaatactg
     3301 gaaactgccg attgtagcga gcggggtcgt ggggcttgct tacgccataa gctggaacgg
     3361 aaaatattat gtagcctata ccaacgccta cagagacatc agcgacaaca accccgacac
     3421 caacagtttt ttggacttac tgccgccggg ctattcctat accgagtcgc aattgcggac
     3481 ggtgctcaaa aatcgtcaac agacctttcg caggcaacgc gatttgagca tcatcggagc
     3541 cgtgggcttt tatttaattt gcatcctgga tgcctacgtc gatgccgagc tgttcgattt
     3601 taacgtcagt cccgacctga gcatccgcct gcaacctgtc cggcaggccc ccggaatcga
     3661 tcacgcaatg gaactatcct gtttattcag gttttaacaa caacgagaaa aaccgattat
     3721 gaaaagaatc atctatatac tgcttatttt aggcttgtct gcgtcgcccg cattatccgc
     3781 gtcacctgaa tcatctgcgt cgcccgcgtc gcctgaatca tccgtgtcgc ctgaatcatc
     3841 cgtgtcgcct gaatcatccg tgtcgcctgc gtcgcccgca tcacctgaat catctacgtc
     3901 gcctgcgtcg cccgcatccg ggcaattttt caaaaaaata ttcactaaaa aagcctcgac
     3961 ggaacaaacc gagggtctcc ccggggaagc aggcaatctg ccgcaacaaa aacaggtgaa
     4021 cggcaaacag accggcaaaa agctgtcgca cagcttgtcc aacccggaga aaaccgggca
     4081 aaacaacctg ttggccgaag aggacgcacc ggccgttgaa atgaacctcg attcaataga
     4141 aaacagcctc ttcagcgaag ggctgaagat actgatcgaa atggattcca gccattattt
     4201 cgacgatccc ggcctggatc cctcttttca tttcaatgcc aacaccgaca gccttattta
     4261 cgaatggcaa aggcagtccc tgctgatttt tgacgaaaac tgccgaccca ctcttacaga
     4321 cagctctgcc acacccgaaa cgacctacat tcaacggctt atggatttac ccaacatcat
     4381 cgaaatgccc tacaacagaa tagtaagacg tttcatcgac ctctatctga acgaaaggag
     4441 gatgcaggta gaagccatgc tgggagcggg agtctattat ttcccactgt ttgaacaggt
     4501 actcgaccaa aatggtttgc ccatagaact caaatatttg cccgtcattg aatcggcgct
     4561 gaatcccaag gctgtttcca gagtcggcgc caccggttta tggcaattta tgtacgccac
     4621 cggcaagatg tacggactcc agatcaacag ccttaccgac gaaaggcggg atccggttaa
     4681 atcgacttat gccgccgtca aatacctgaa gagtctctac tctatgtttg gcgactggaa
     4741 cctggccata gcagcctaca actgcggccc gggcaatgtg aacaaagcca tacaccgctc
     4801 aggcgggaaa cgcaattttt gggaaatcta ctattaccta ccccgggaaa cccgcagcta
     4861 tgtgcccctg ttcattgccg ccaattatgt gatgacctat tattccgaac acggtatttg
     4921 tccggcccaa aacaattttc ccccggtgat tgatactttt atgatccatc aaaacatgca
     4981 cttccagcaa attgccgaag tctgccagat accgatgcag gaaatacaat tattgaatcc
     5041 ccatttcaga aacgagattg ctcacggcac cgcctccaag gccggtatac tctacctgcc
     5101 tatggaatat tcgcccgtct ttacagaatt gcaagacagc attccccgtt acaaagaaca
     5161 ggaattgctg gcttcgaggc tggtttcggt tcaatcggga caagcggggc caaccgatgc
     5221 cattgtttac agggtaaaac ggggcgacaa cctctcggta attgcatcaa gacacaacgt
     5281 aagtgttcaa aagctgaaaa actggaataa ccttaagagc aatacgttgc aaattgggca
     5341 acgactgctt atttattaac gattaaatag taccttcgca taaaactctg cacagaggcc
     5401 gattatggat attttacagg caacaaatcc tgaagaagaa aaaatctttg agcaatttca
     5461 gcttttgctc aacgattacc tgaattcaaa ccatcgcaaa aaagtggatt tgattaccaa
     5521 ggcatttaag tttgcttaca atgcccatag aggtgtcaaa cgccgctcgg gagagcctta
     5581 tataatgcat cctctggccg tagcccgcat ctgttgcgcc gaaatcggtc tgggttccac
     5641 ctctatctgt tccgctcttt tgcacgacgt gatagaagac accgagttta ccgtcgaaga
     5701 catccgaaac aacttcaacg acagaattgc ctccatagtg gaaggtttga caaagatctc
     5761 cggaggaatc tttggggaga acgcttccac tcaaaccgaa aatttcaggc gcctgctcct
     5821 gaccatgtcc gaagacatcc gcgtaatcct gataaaaatg gccgaccgcc tgcacaacat
     5881 gaggacccta agcgccctgt cgccaagcaa acaatacaaa attgccagcg aaaccatgta
     5941 tttgtatgct ccgcttgccc accgtctggg attgaaccgg atcaaaaccg aactcgagga
     6001 actgagtttc aaatacgagc atccggaagc ttacgaacaa atcacacata aactggccat
     6061 cacggccgaa tcgcgaaagg cactatacta ttcctttata gaacccgttc agcacgaatt
     6121 ggataaactg gacttgaaat actacatcaa ggaaagagta aaaacagcct actccatctg
     6181 gaagaaaatg accaccaaaa acatccggtt cgaagaaatc tacgaccttt tggctgtccg
     6241 tatcattttc gattcgcccg atattgagga cgaaaaagag atgcttgccc tcgaaaagaa
     6301 acaatgctgg gatatatatt ccaccatcac caatatttac aagcctcacc ccgaccgttt
     6361 acgcgactgg gtaaacaacc caaaagccaa tggttatcaa gctctgcaca ccacggtgat
     6421 gggctttacc ggcgattgga tcgaagtcca gatccgcagt accagaatgc acagcattgc
     6481 cgaaagaggt tttgccgccc actggaaata caaactgggc gaaatcgaag aagataacga
     6541 gctcaaccac tggatactca ccatcaagga gattttggaa agccccgagc ccaacaccct
     6601 cgatgtgcta gacaccatca agatgaacct gttttcgtcc gaaatcttcg tgtttacccc
     6661 caagggagaa atcaaaaccc tggctcaggg agccaccgcc ctcgattttg cctttgaatt
     6721 gcacagtgaa ctgggctacc gctgcatagg cgccaaagtc aaccatcaac tggtacccat
     6781 gagccatgtg ctcaaaagcg gtgaccaggt ggaaattctc acctccgaaa accaaactcc
     6841 ccgctacgaa tggctgaaat ttgtcaccac cggcaaggct cattccaaaa ttaaagcccg
     6901 ttttcgcaaa gacaaccgtg agacaataaa aaaaggtgaa gagctgctca atgaattttt
     6961 caacaagcat catttaacag cagacgccga caatgttcag gtattgctga ccaactgtaa
     7021 ttgcatcaat aaggaagaac tcttgatgaa gctgggtacc ggcgaattga ctctaaacaa
     7081 cgccgaagcc gaactcttca aaaaggacaa tcagaacctg ctggtcaaat actggaaact
     7141 taccttcgga tcgaagaaca gcaagcagga aaaagaaaaa ggaaaagaaa aagaaaaaga
     7201 aaacaaaacc atcgacttca aatcgacaca ccggcttacc gaagaatccg ttcagaacga
     7261 ataccacctg tcggattgct gtcagcccat accgggagac gccgtactgg gttacgttgc
     7321 ccaaaacggc attattcacg tacacaaacg gcaatgcccc attgccatgc gcctgaaaag
     7381 cagctacggc gaccgcatcg tttctgccga atggggcaca cacaaaaaat tatctttcgc
     7441 cgtcgaaata gaaattaccg gcctcgacac cgtaggcatg atcaatcaga tcacccgcat
     7501 cgtctccgac gaattctcgg taaacatcaa caaactcaac ttcgaatcca aagacggcgt
     7561 attccgaggc tccatgaccg tttacgtcca cgacgtagaa ggcctgaacc acctctgcaa
     7621 caaaatctcc aaactcaaaa acatcaaatc cgtcacccgc gtcgaacgcc aaaataagct
     7681 gatgggggtg aaggagtgaa tttttggagg gggtggtggg agattatgga gagatttttg
     7741 tgcctcgaaa tctaaaatta atacaaaaat aacgatatga ctccaaaatg agcacagaaa
     7801 aataaagcgc atcagatata aattatttaa cttgcggatg ttttaatgta aaaaaatgag
     7861 atttaaatta aaattagcct tagatccatt gccttacgga aacacaatcc ctgtcaacta
     7921 ccaatacgaa ctatccggat ggatttatcg tacgttggct agcggggatc agatgaaaaa
     7981 atattagtgt taaaaaagat ataatcgata tatatcatga gaatacattt aaagtttaaa
     8041 atttcaaatc aaacagttcc ttttgaccat caacctttgt tagtgggagc tatccataaa
     8101 tggttggggc agaatgagga acacggtgaa atctcattgt actctttttc tcgattggag
     8161 ggaggaaaag caacaaatga aggaattaaa tttaatcagg atggattatt tttttttagt
     8221 tccatcaata tagatttagt gaaaagattg atatccggaa tccgatcaga tccttcaatg
     8281 ttttttggac taagagtaaa tgaattattt attgaagaag accccgattt atcaaaccga
     8341 gaattatttt ttccgggtag tccgatattc atcaagagaa ggaatggtga aagaattgaa
     8401 caaatttttt ttgacgatcc aagatcttcg gaatacttga gagaaacact tctaaccaaa
     8461 atgggaaaag tcggacttga ggacgattca cttgatattc gttttgattt gtcccatacc
     8521 aaatcgggaa caaaaatgat cacttataag ggtatcaaga ataaagcaaa ttggtgtcca
     8581 gtaattataa aaggcaaacc agaaacaaaa ctttttgctt ggaacgtcgg attaggaaat
     8641 tcaacgggaa tcgggtttgg agcaatcaaa taataggcag ttatgtatta tgttgtaaaa
     8701 tattcaggtc catttgggtt tattaaaccg tggaccgctg tgagagacag tgagacttat
     8761 agccaacagt ttctaactcc ttcgattatt gaaggaattg agaagaaatt gtttcccgag
     8821 ttgttacata aaaccgggaa aattctaaaa attgttcgat accggttgaa ttattgtggt
     8881 attgacatgc agcaagaacg aactagatcg aagggtgaat ttgaaaaaga gaagaaaaca
     8941 gattcgttag gtagaacata ttacacagcg aagaacatgg gggtcattac tcgaggtgtg
     9001 ctacttcagc ccaatcttta ccttgcgttc aaggatgagc aggatgcgaa caatgcattt
     9061 gcccaaacga tatgtttgtg tagaaacgag gatttgcttt ttcctgaaat tctcacggaa
     9121 atgtcagaag aggactttga caaaatcgat ggttttgagc tagagacttc ctctcctgaa
     9181 agcggcttta aggttggata taatcgtttt gatgattttg cggagatgtt tggcgagctc
     9241 aaggttgtgg gcaatccaat acgtgatcga taatgaaacc ccttgatcaa atactagcta
     9301 aaagtgtcaa ttacggcaat ataagtttgc tggaacatac acaagaggta acactggcaa
     9361 ttgaaaactt tgcccggcat tttgatttta catttaattc agagctcgct cgtaaaggag
     9421 ccatcttgca cgatctgggc aaagctcatg accattttca aaaaaaaata cagaatatta
     9481 attcaaaatc attagccgaa gcaagggaag aaaattatat tcacagacat gaactttcgt
     9541 cattggcttt tctacctgtt tttccgaaag aggaatggga ttatttaatt gatatgattg
     9601 ttgcccacca caaatcaatc gaaaacgacc ctaggggaag aggtattctc gatttagatc
     9661 agaatagcca atattggata aataaccatc taaaaaactg ggaagaatgg agccaatatg
     9721 gtttatgtat tcttaatata tttggataca aaagcaatat aattacttta gatgaggcaa
     9781 gaaatgcttt actatataca gttgagtatt gtgaatcaaa gggaaacgga tggtctcctt
     9841 ggcgtggttt gttaatttct gctgaccatt ttgcatctgc ttttacaaat aaaacagcag
     9901 agaatcttca acatcttttt gaaacacccg atttgagcta ttatcacgat ttatcgagaa
     9961 gaaatcatct ttatccctta tcagaaatct caacagaaga ttcgcgtctt cacacactag
    10021 ttgttgctcc tactggagca ggtaaaaccg acttcctgct aaaaagatgc aaagggcgta
    10081 ttttttatac attaccattt caggcttcta tcaatgctat gtgggaaaga atgaaaaaca
    10141 cgattcctaa taaagatatc cgactcttac atgcaatgtc taaaattatt gttggtaaaa
    10201 atattgaaga gcggattctt caacctttag ttggttcaac ggtaaaagtt cttactcctt
    10261 accagctcgc cgccattgtt tttggaactt ctggttttga aggtgtaatg cttgatgtga
    10321 agggatgcga cattattttg gatgagattc atacctattc cgattattct aaatctatgg
    10381 ttttggaaat tgtgaaaaca ctattgcgtc ttgactgcag aattcatatc ggtacagcta
    10441 ccatgccatc ggtgctttat aatgaggtac tttccatttt aggtggaaaa gataaagttt
    10501 acgaggtgaa attgccaggt gatgtcctaa agtcatttaa tcgtcataag atttttaaaa
    10561 ttaatagtga tgatgagatt cccaatatcc tggatcaggc atttcggaac agagaaaagg
    10621 tcttggttgt tttcaacaca gtaaaacaag cccaggatgc tttcaaaact ttttcagaaa
    10681 gatttactaa aataccgatg atgcttatac atagccgttt tagacgaggt gatagagttg
    10741 aattggaaac ccgacttaaa aggcaattca atggagacgg aagtaaagaa tttggagatg
    10801 gattatgccc gtgtttggtg gtttccactc aagttgttga agtaagtctt gatataagtt
    10861 ttgaccgcat aattacccaa tgcgctccac tcgatagtct tatccaaagg ttcggaaggg
    10921 taaaccgtaa acgcagcaag gataccattg gaaaatataa acctgtacat attatcgcac
    10981 cacatgataa agttttaccc tataaaatgg atgtgctaaa aagaagcttt gaacaattac
    11041 cagacgatgg gacaattctt aatgaacaaa acttacagga aaaaattgac aatgtgtatc
    11101 ctgttataga aacaaaagaa atagatgttc acttgatcta tagaaacaac cgatattcat
    11161 tacgggaact tactaataca aaaaaagctg ttttagtgga agctttagaa attgaaagtg
    11221 caacctgcat tttagaaacg gatagggaaa aatatctgtc agcagattgg gaagatcgaa
    11281 tatacatgga gattccaatt aattggaaaa ccatggaccg ttacaaatct gaatacgaac
    11341 agctagacgt tggggcaaac ccatttgttg tccctcagaa cttaaatgat tatcgaatat
    11401 ttggattgcg actagttgaa aacgataaat ttttataggc atgtacacaa gttatatagg
    11461 caagaaattt ctgaaactct acaatgagag agagggtaca aacctctcag ctgaagagtt
    11521 ttttgaaaaa gtgtttttcc cacttttctt tgatgatgac agacatttac tgcatgtcgg
    11581 aaattctccc ttttttcaaa agctcaaaga agaagatatc aaaaagtatg ggagcaaatc
    11641 attggctcag tataataatt taaaaaaagc aattaaaaat gacattccca atatgtctat
    11701 ttttgtgggt tccgccgcaa aagatgtaaa aggaacgact tccggccaag tttcaaatat
    11761 taatgttatt gtagataaag aagaaatgta tgcctcatgg atgggtgaag ctctaggtat
    11821 tcgtattagt ggaggaatga ttatgctaat tgatgaaccc gatgttttat gggcattata
    11881 ttctggatgg aggcattacc gtaattattt aaatcaaaca cctaacgtag aagacaaaca
    11941 aattgagaca tggaatggac aatggctttg gcattggtcc caacctcatt ttgacaataa
    12001 taaccctctc gatagattac acatagaagt ttcagaagta caaggaaatg tagcaatctc
    12061 cactcaacaa tggataaaag gaatatttgc tttatcaagg aaatttccga ataaagtaat
    12121 cacggcttat gcatataacc tttcgcaaac caacataaca ttaggcttta ttaacttata
    12181 tctaccagag gtaaatcgat tgtttaaact gcgcaataaa atatttatag atgaacccag
    12241 aaccattctc actgaagaag agattaattc atttactacc ttttataatt tcaagtgcgc
    12301 ctgcaaattt ggaacaattg gcctcaaggc tattgagcct gcaaagctta gagaatatat
    12361 gcctattggt tctgtctcat ttgcaaaagg aaaagagtat aagttttcga acgaagaatc
    12421 atatacaaat tataaacttt ttaaaatttg gattattgct atgctaaaca aaacagaatt
    12481 actcaagtta gcctccgatg ttgcaaaagc tttatcggaa ttagataaag ctgacgacag
    12541 ggggaaaaaa gtatttgcaa ctttatctca agaagttaga gaatctaata atttacggtt
    12601 gtttattgat aaacttacta cggtgctaaa ccaagcaccc tcaaatgcgg aaacgtttaa
    12661 agaagtggtg gaagctgtct tgaaaatgcc tttggacaat tttccactgt ttgttacgct
    12721 tatccgcttt gaatatgctt atttaaaatc aaaaaactaa ttttaaaatt tatagatatg
    12781 agctgttcac ctttcattta tttacgtggt cttcgtcacg cggaacatac tgtttttggc
    12841 gttaatgatg gacaaaagtt ttattatgac cctcagtttg ggaaaaaaat ggcttattca
    12901 agtggccaac aagttaaacg atccataatt gaagctttga atatgccttt tgctgcaata
    12961 acattcaatt gggaaataga tagaaaagaa aacaaggcaa gtcaaaaaga accacattct
    13021 ccgtgtgatc cctcttatcc agaccaatta ttgggtggtt atatgaaagc agaaagcgga
    13081 aatatgacgg ttaaaagaag aagtccatta tcaatctcgg ccatgcgacc tttacatcca
    13141 ttgcttggag gaatagaaaa tccattagaa gacttaacat tcgatcgaac ttcacaccca
    13201 gaccatcatc aagtaaaagt tttttggctt gacaataaaa agagaggagc cgaattgaca
    13261 tcggatgaat tagaagaatg gctaacgact aatcgcagaa gccttcctaa tagagctttt
    13321 atacagaacc aatctcgtgc ctctggttta ttcgtttatg atattgctat agacctccgt
    13381 actttatttt gtgtttcaac aaacaaatta gaacctgaac tcttccctga aatagaaaca
    13441 aaacttcgta atgaagggtg gatagaaggt ga
//
LOCUS       DAOSGL010000018        13371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_3866_length_13371_cov_4.458396, whole genome shotgun sequence.
ACCESSION   DAOSGL010000018 DAOSGL010000000
VERSION     DAOSGL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3866_length_13371_cov_4.458396"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKY68_01290"
     CDS             <1..452
                     /locus_tag="PKY68_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOH23705.1"
                     /translation="QIFAVSLDFSPLDKMQKKQVLDIVTKELLTPKGLRTLSPKSLGY
                     NPIFQGSESRRTWAYHQGTAWAWLIGPYVEAYLKVHKIRGLNFAERIIYGFEDDLTDR
                     GIGTISELYDGNPPYWGRGAVSFAMSVAEILRIKQIIKVMSANTTQG"
     gene            449..1714
                     /locus_tag="PKY68_01295"
     CDS             449..1714
                     /locus_tag="PKY68_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH23706.1"
                     /translation="MRALMFGWEFPPHILGGLGTASYGLTKGMAMQEDMDLVFVIPKP
                     HGDEDQSFVKIIGACNTPVVWRDVSWEHLESRFGSYMSPQDYYNMRDHIYADFRYLYT
                     NDLGCIEFSGRYPDNLMEEINNYSICAGVIARTEQHDIIHSHDWLTYSAGIHAKNISG
                     KPLVIHVHATDFDRSRGNVNPQVYGIEKNGMDHADHIICVSNLTRETVISKYHQDPRK
                     VSAVHNAVEPVSKAIEEIQPERHTNQKIVTFLGRITMQKGPEYFVEAASRVLQKTSGV
                     RFVMAGSGDMMEAMIKLVAKRGIADKFHFPGFLKGNQVYRMLKSSDVYVMPSVSEPFG
                     ISPLEAMQVGVPSIISKQSGCAEILNYAIKTDYWDIDAMADAIYSIITNPAMHEFLKI
                     QGKNEVDEIKWEYAGKKVRAIYDRLLGIK"
     gene            1732..3093
                     /locus_tag="PKY68_01300"
     CDS             1732..3093
                     /locus_tag="PKY68_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOH23707.1"
                     /translation="MKTICFYFQIHQPFRLKRYRFFDIGADHYYYDDFTNEQILQRIA
                     VNSFLPANQIIYDLIQSSEGKFRVAFSISGVALDQLEQHAPEVIESFRKLAKTGAVEF
                     LSETYAHSLSSIVDEKEFKNQVTQHRKRIEVLFGQKPKTFRNTELIYSDDLGELIYSM
                     GYKTVIAEGAKAVLGWKSPNFVYHSALNPNLKLLLKNDKLSDDIAFRFSNYNWSEYPL
                     VADKFIDWIQKTPEKEQVINLFMNYETFGNLQPKESGIFEFLKALPKFAFKKGIEFST
                     PSEVSSLVKATGPISVPNPTSWTDEERDLSAWLGNDLQQEAFNKLYSVAERVRMSKDR
                     RLQLDWNYLQSSDHFYYMSTKSFSDGGLYRYFSPYESPFEAFINYMNVLSDFMERVNA
                     QFPSSVGNEELNSLLTTIQNQELKIADLEKQLKKYKKESPTGNRQVAVKKGGAVKKGN
                     KAK"
     gene            complement(3120..3668)
                     /locus_tag="PKY68_01305"
     CDS             complement(3120..3668)
                     /locus_tag="PKY68_01305"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOH23708.1"
                     /translation="MENPSVQKEKLRKTLCLLTKQQDPAELDKESRLICNKLEGSPEF
                     KEAQNLLLYYSLPDEVSTLELIRRWYKHKNILLPVIVNESTMVLRLYTGKNALQLNRW
                     GITEPTGEDFLIYDEIDLAVIPGVAFDKQGYRLGRGKAYYDRFLGRLRCPVFGLCFAH
                     RYLESLPVEAWDIPVQRVISSI"
     gene            complement(3684..5309)
                     /locus_tag="PKY68_01310"
     CDS             complement(3684..5309)
                     /locus_tag="PKY68_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH23709.1"
                     /translation="MKNRYQLFLIVFLSVLIGITIGNFVSLRQQSKGIPSLVNFNKLD
                     ALLHLISSKYVDDINEKELIEGLIPKVLLELDPHSVYITAEEMERANEDIEGSFSGVG
                     IQFNIREDTIRVIAVITGGPAEQVGLRAGDMIVSINDSSFVGPSITNENVMKTLRGEK
                     NTKVKVGIRRRNAPQELSYNLTRDDIPVNSIDASYKIGENIAYIKLGKFTRSTYNEFL
                     SVISKMQAQDNCDRVILDLRGNPGGLMGPALAILNEFLPRNSLMLYVEGKAYKREESF
                     SDGRGSCQNTPIIVLMDEWAASSSEIVAGAIQDNDRGYIVGRRSYGKGLVQQQIPFRD
                     GSAVRLTVAHYYVPSGRNIQKPFEKGDFKDYEMDLINRYLRGEFDSRDSIRLSDSLVY
                     KTKGGRPVYGGGGIMPDFFVPLDTTGGSEWYNTVFNQIFPYNFAASYADHHREQLLTF
                     KTPAALKAYLQEQRVFENFVDYTVAQGIPKKPQEISESKDLAADQIYAYIARNILGED
                     AFWMILQEKDPTLNKAVSLIQNLGTNVSLDHTR"
     gene            complement(5334..5762)
                     /locus_tag="PKY68_01315"
     CDS             complement(5334..5762)
                     /locus_tag="PKY68_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOH23710.1"
                     /translation="MNEKQALLDKRYMRMALIWAENSYCKRRQVGALMVKDKRIISDG
                     YNGTPSGFENICEDSEGNTKAYVLHAEANAITKVARSHNSSQGATIYVTACPCIDCAK
                     LIIQAGIVRVVYGEPYHAEDGLNLLLRAGIDVCFCDKSLL"
     gene            complement(5763..7847)
                     /locus_tag="PKY68_01320"
     CDS             complement(5763..7847)
                     /locus_tag="PKY68_01320"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOH23711.1"
                     /translation="MKTKLFFIMTALTVIGLQGSNPFLGKYKTLHETAPFREVKIEHI
                     LPAYQEAIRRHDNEIQKITANKEPASFANTIEALERSGELLDKISAVYYTLLNNQTCD
                     ELMNLSQQISPLLSEHHNNIVLNDALYQRIKTVYDNRENLNLNTEQSRLLWETYMDFL
                     DSGATLEGADREKYRELSTKLSQLSLNFGQNVLKATNAYSKVFTDKTLLSGIPEDVLS
                     IAAEKAKAKGLEGWLFDLTAPSYGAFLKYADNRELREEMYRAYMGRGLDGEFNNQPII
                     RDIVNARLEMAGLFGYPDYASYVLRRRMTKNSETVYGLLDQLREAYLPLGQRELSALQ
                     GFALGYEKNFIKLEAWDWSYYSQKLKDLQFNLNDEMLKPYFKLDNVIEGVFGLATKLY
                     GLSFKKNNKIQVWHEEVTAYEVFDKDGSFLAVLYTDFHPRASKRSGAWMSYIKAQHKI
                     KNKDIRPHITLSTNFTPSTSEKPSLLTFDEVTTFLHEFGHALHGMLSNTTYASLSGTN
                     VYRDFVELPSQIMENWGTKQEFLNTFAVHYQSGEQIPAKLIEKIIAADNFNVGYACLR
                     QLSFGYLDMAWHGLQKPFDGDVRQFENQAWSSVILLPQPGEACMSTSFNHIFSGGYAA
                     GYYGYKWAEVLDADAFEYFTENDVFDTERAASFRENILSRGGTEDPMELYLKFRGREP
                     RIDALLKRNGIK"
     gene            complement(7970..8368)
                     /locus_tag="PKY68_01325"
     CDS             complement(7970..8368)
                     /locus_tag="PKY68_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOH23712.1"
                     /translation="MSRFELFLKYPLFKGMSMDELFPLLTKVSLDFDNYTAGDLIFNK
                     DIVCENLVFLLDGQVELENEQKEKRIAQGPELIVYTRLFGRKRNMPLTARALDNCSIM
                     QIDRKSLLFLMRNSQTVLCNYLDLISDMIA"
     gene            8455..9042
                     /locus_tag="PKY68_01330"
     CDS             8455..9042
                     /locus_tag="PKY68_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOH23713.1"
                     /translation="MKINLKEILSAFTVLFAIIDIIGSIPIIIDFQKKPGGVPALKIS
                     LISFALLIGFFFLGDGVLALFGVNISSFAVAGAIVIFAVAVEMTFDIEVFRHKGPPEV
                     SSIVPLVFPLIVGAGSFTTILSLKAEFQAINIMIALFLNMVVVFVVLKGIKRIEKFIG
                     NAGIYIARKFFGIILLAISVKLFTTNLGSLLSQFK"
     gene            complement(9117..9407)
                     /locus_tag="PKY68_01335"
     CDS             complement(9117..9407)
                     /locus_tag="PKY68_01335"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOH23714.1"
                     /translation="MRRKNTQRLAEVLDEVLKNQQLRGKLNETAAIRLWPEVVGKALA
                     EHTDNLYFKHSVLHVHVKSAIIRNELMLIRPQLLKRLNEKIGSQAVKNIMLH"
     gene            complement(9418..10515)
                     /gene="recF"
                     /locus_tag="PKY68_01340"
     CDS             complement(9418..10515)
                     /gene="recF"
                     /locus_tag="PKY68_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852665.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOH23715.1"
                     /translation="MYLQKLSVFNYKNIQEANIEFSPRLNCFFGNNGVGKTNFLDAIY
                     YLSFCKSHTNPIDSQNIFHEADYFVLQGNYLIGGKEEEYYCAMKRRQKKIFKFNKKEY
                     ERLADHIGRLPLVIVSPADENLIREGSDERRRFMDIALSQFDRSYMEALMSYNKALYQ
                     RNALLKNEQMNKEDSLYEMYDAPMSDYAGLIYQKRREFIAAFQPVFEHYYHSVSDRSE
                     SIGLQYRSHLEQGNLQEQLLRSREKDIILGFTTKGIHKDELEMQIDGYPIKRVGSQGQ
                     NKSYLIALKLAQYEYLKQAGKKKPILLLDDLFDKLDASRVERIIQLVSKEDFGQIFIT
                     DTHREHLNMILQDNGKDFRVFHIENACICPI"
     gene            10633..11331
                     /locus_tag="PKY68_01345"
     CDS             10633..11331
                     /locus_tag="PKY68_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH23716.1"
                     /translation="MAKKKVSKKAAQKEELQNVGEILTRSEQFIENNQKSLLVALLVI
                     VVVVSGIILFRNSYMGPKEKEAQEMMFRGEQYFAADSFELALYGDDYEFIGFEGIIEN
                     YGITKTAKLASAYAGLCYKHLGDYEKAIDRLNKLNAADIMVSPALTGAIGDCYVELDQ
                     LQKALPYFERAGQSDNDLLAPIYLMKAGRVYEALGKYDSARGVYEKIKKNYPLSQEGL
                     NVDRYIERTRLQKK"
     gene            11328..11822
                     /gene="ribH"
                     /locus_tag="PKY68_01350"
     CDS             11328..11822
                     /gene="ribH"
                     /locus_tag="PKY68_01350"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOH23717.1"
                     /translation="MSTKNLSEYNPDTLPDASDMKIGIVVSEWNYDVTVRLLEGARQF
                     LLRNNVDPENIHVAWVPGSFELIYGAAKMEASEPVDAVIALGSVIRGETPHFDYVCSG
                     VTQGIARLNAKGSIPFIFGLLTTDDMQQALNRAGGIHGNKGVEAAKTAIQMIYFGRQL
                     DYLD"
     gene            complement(11885..>13371)
                     /locus_tag="PKY68_01355"
     CDS             complement(11885..>13371)
                     /locus_tag="PKY68_01355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOH23718.1"
                     /translation="IIDEVHMLSSSAFNAFLKTLEEPPAHAIFILATTEKHKIIPTIL
                     SRCQIYDFHRIGINDICAYLQYVADSEGIETEPEALNAIALKSDGGMRDALSIFDQVS
                     SYGEGKITYQHVIDNLNILDYDYYFKLVEAFLNENTTESLLLFDQILNQGFDGLHFIN
                     GLASHFRDLMVSKDPRSISLLEQAESVRVKYKKQAEECSMGFLFKALKLSNECSLQYN
                     QSKNKRLLVELCLIQLCLTGEAKKKQGELKTDKIAAAKVEKNPLPSSKTQTQANPSSP
                     SITANPKQATLTDSTPQPQSQPQPQPNPQPGNTVEGNKEAQTHQQAAVPEPAPQNQNP
                     STGTSKERSLPGLTTSIRRRKIQADDTSGQEATTKEAVKTEEHFSEENLIEAWLRFAG
                     TLKDDPYLSNSMQACKPRLLNKSDFEVVVFHPLTAQRLEAIRGDIESSLQRDLKNGKI
                     HMLLRISEEDELKKPLSPQDQLDEMIKKNPNLQSLKQKLSLELQ"
BASE COUNT         3548 a         2828 c         3121 g         3874 t
ORIGIN      
        1 atcagatttt tgcagtctcg ctcgattttt cacctttgga caagatgcaa aaaaaacagg
       61 tactcgacat tgtcaccaag gaattactca cacctaaggg cttacgaaca ctcagcccta
      121 agagtttggg atataatcct atattccagg gatccgaaag tcgcaggacc tgggcatatc
      181 atcaagggac agcctgggcc tggctgatcg gtccatatgt tgaagcttac ctcaaagttc
      241 ataaaattcg cgggttaaac tttgcagagc gcattattta tggctttgaa gacgatctaa
      301 ctgatagagg tataggtact atttcggaac tgtacgacgg aaatcctccc tactggggca
      361 gaggagcagt ctcctttgcc atgagtgttg cggagatact ccggataaaa caaataatta
      421 aagtaatgtc agctaatacc acacaaggat gagagcactt atgtttggct gggaattccc
      481 tccacatatt ctgggcggtt tgggaacggc cagttatgga ttgaccaaag gaatggccat
      541 gcaagaagat atggatcttg tttttgtcat tcctaaacct catggagacg aggaccagag
      601 ttttgttaaa attatcggag cctgcaatac gccggtggtt tggagagatg tttcctggga
      661 acacctggaa agtcgtttcg gttcttatat gagtcctcag gactactata acatgaggga
      721 tcatatctat gccgatttca ggtatttgta cactaacgac ctgggttgta tcgagttttc
      781 cggacgttac cccgacaatc tgatggaaga aatcaacaac tattccattt gtgccggggt
      841 tattgcccgt accgagcaac acgacataat ccattcgcac gattggctca cctattctgc
      901 aggtattcat gctaaaaaca tcagcggtaa acctttggtc atccacgttc atgctaccga
      961 tttcgaccgc agccggggca atgtcaatcc acaggtctac ggcatcgaaa aaaacggcat
     1021 ggatcacgct gatcatatta tttgcgtcag taatctgacc cgagaaactg tgattagcaa
     1081 ataccatcag gatccccgca aggtgtctgc cgttcataat gctgttgagc ctgtgagcaa
     1141 agcgatagag gaaattcaac cggaaagaca tacaaatcaa aagatagtaa ctttcctggg
     1201 taggatcacc atgcagaagg gaccggagta ctttgtcgaa gctgccagcc gtgttttaca
     1261 gaaaacctcc ggtgtccgct ttgtaatggc gggcagcggc gacatgatgg aggccatgat
     1321 aaaactggta gccaagaggg gaattgcaga caagtttcat ttcccgggct ttttgaaagg
     1381 caaccaggtt tacagaatgc taaaatcgag tgatgtttac gtaatgccgt cggtgtcaga
     1441 gccttttggg atatctccgc tcgaagccat gcaggtaggg gtcccctcca ttatttcgaa
     1501 acaatccggt tgtgccgaga tcttgaatta tgctattaaa accgactatt gggatattga
     1561 cgccatggcc gatgccatct attcaataat caccaatcct gccatgcatg aatttttaaa
     1621 gattcagggc aaaaatgaag tggacgagat caaatgggag tatgccggga aaaaggtcag
     1681 agctatttac gaccgtctgt taggaataaa gtaattatta aataaagcct tatgaaaacg
     1741 atttgttttt actttcagat tcaccaacct ttccgcctta aacgatatcg cttttttgat
     1801 ataggtgcgg atcactatta ttacgacgat tttaccaacg agcagatact tcaacgaatt
     1861 gcagtcaatt cctttttgcc ggcaaatcag attatctatg acttgattca gtcgagtgag
     1921 ggaaaattcc gggtggcttt ttccatttcg ggcgtagcct tggatcaact ggagcaacac
     1981 gcccccgaag tcatcgaaag ttttcgaaaa ttagccaaaa ccggcgcagt cgaatttcta
     2041 agtgagacct atgctcattc tctgtcttcc atcgtagatg aaaaggaatt taaaaatcag
     2101 gtcacgcaac accgcaaacg catagaagtc ttatttggcc aaaagcccaa gacttttaga
     2161 aacaccgaat tgatctattc cgacgattta ggagaattga tctattccat ggggtataaa
     2221 accgttattg ccgagggagc caaagccgtg ctcgggtgga aaagccccaa ttttgtctac
     2281 catagcgctt taaaccccaa cctgaaactt ttattgaaga acgataagct gagcgacgat
     2341 atcgctttcc gtttttccaa ttacaattgg agcgagtacc ccttggtggc tgacaaattt
     2401 atcgactgga ttcaaaaaac gccggagaaa gagcaggtta ttaatttgtt tatgaattac
     2461 gaaacctttg gcaatctcca gcccaaagaa agcggcattt ttgagtttct caaagccctg
     2521 cccaagtttg cctttaaaaa agggattgag ttttcaacac cttccgaggt gagtagtctg
     2581 gtaaaagcaa caggccccat cagtgttcct aaccccacct cctggacaga cgaagagaga
     2641 gatttgagcg cctggcttgg gaacgacctt cagcaggagg ctttcaataa gctctatagc
     2701 gtggccgaaa gagtgcggat gagcaaagac cgccgcttgc aactggattg gaattacctg
     2761 cagagcagcg accattttta ttatatgagt acaaagagtt tttcggatgg tggcctgtat
     2821 cgttacttca gcccctacga gagtcccttt gaggctttta taaactatat gaatgtgctg
     2881 agcgacttca tggagcgagt aaatgcccag ttcccctcat cggtgggaaa cgaggagtta
     2941 aattctttac tgaccaccat acaaaaccag gaacttaaga ttgcagatct cgaaaagcaa
     3001 ttaaagaaat acaaaaaaga aagccccacc ggcaaccgtc aggtagcggt taaaaaggga
     3061 ggagcggtta aaaaggggaa taaagcaaaa tagttgagcg gcgggcacaa tcaaaaactt
     3121 caaatgctgc ttattactcg ttgaaccggg atgtcccagg cttcaacggg caggctttcg
     3181 aggtagcggt gggcaaaaca taagccgaac acagggcaac gaagccggcc cagaaaacgg
     3241 tcgtagtatg ctttaccccg tcccagccta tagccttgtt tatcgaaggc cacaccggga
     3301 ataacggcca ggtcgatctc gtcgtaaatc aagaaatctt ctccggtggg ttcggttata
     3361 ccccagcgat tcagttgcaa agcatttttt ccggtatata gacgcaagac catggtgctt
     3421 tcgtttacga ttacagggag cagaatgttt ttgtgtttgt accagcgtcg gatcaattcc
     3481 aaagtactca cttcgtcggg caaggaataa tacaacaaga ggttttgggc ttctttaaac
     3541 tcaggacttc cttctagctt gttgcaaata agcctggatt ctttatcaag ctctgcgggg
     3601 tcttgttgtt ttgtaagtaa gcacagagtc tttcttagct tttctttttg cacggacgga
     3661 ttttccatct ctatttaccg gacttaccta gtatggtcaa gagaaacgtt agtccccaga
     3721 ttttgaataa gcgaaacagc tttgttgagc gtgggatctt tttcttgcag aatcatccag
     3781 aaggcatctt ctcccaggat attacgcgct atataagcgt aaatctgatc agcggctaaa
     3841 tccttggatt cggagatttc ttgcggtttt ttgggaatgc cctgggctac cgtataatcg
     3901 acaaagtttt cgaagaccct ttgctcttgc agataagcct ttaaagcggc aggagtttta
     3961 aaagtcaata attgctcgcg atgatgatcg gcgtaactag ccgcgaaatt ataggggaaa
     4021 atttggttga acacagtatt gtaccattca ctgcctccag tggtgtctaa gggcacgaag
     4081 aaatcgggca taatgcctcc tccgccgtaa accggacgtc ctcctttggt tttgtaaaca
     4141 aggctgtcgc ttaaccgaat actgtcgcgg gaatcgaatt ctccccgtag atagcgatta
     4201 atcaaatcca tttcgtaatc tttgaaatca cccttctcga aaggtttttg gatattacgt
     4261 cccgaaggta cgtaataatg cgcaaccgtt aaacggacgg cagaaccgtc cctgaatgga
     4321 atttgttgct ggaccaaacc tttcccatat gacctgcggc cgacaatgta tcccctgtcg
     4381 ttgtcttgta tggctccggc tacgatctcg ctcgaagagg cagcccattc atccatcagt
     4441 acaataattg gagtattctg acaagagcct ctgccatcgg aaaaactctc ttctcttttg
     4501 tatgccttgc cttcgacgta cagcattagg ctgtttcggg gaagaaactc attcagaata
     4561 gccagggcag ggcccattaa ccccccggga ttgcctctga ggtcgagaat aacgcggtcg
     4621 caattgtctt gtgcctgcat tttgctgata acagacaaaa actcgttgta ggtggatctg
     4681 gtaaatttac cgagtttaat gtaagcaatg ttttcgccaa tcttgtaaga agcatcgata
     4741 ctgtttacag ggatatcgtc tcgtgtaagg ttgtagctga gctcctgtgg ggcatttctt
     4801 ctgcgtatac ccactttgac ttttgtgttt ttttcgcctc tcagggtttt cattacgttc
     4861 tcgttggtga tgctcggccc cacaaaactg ctgtcgttaa tgctgacaat catatcgcct
     4921 gctctgaggc ccacttgttc ggcaggcccg cccgtgatga cggcaatcac tcgtatggta
     4981 tcttcccgta tattgaattg tatgcccact ccgctgaatg aaccctcaat gtcttcgttg
     5041 gctctttcca tttcttctgc cgtaatgtag accgaatgag ggtctaattc caataaaact
     5101 ttgggaatca gaccttctat gagttctttt tcgttgatat cgtctacgta ttttgacgaa
     5161 atgaggtgga gcagggcgtc cagcttatta aaattaacca aggagggaat gcctttactt
     5221 tgttgcctga gagaaacaaa attacctatg gttataccga tgagtacgga aagaaatacg
     5281 ataagaaaca gttgataccg gtttttcatt gccaggattt attaaatagt taattaaagc
     5341 aggcttttat cgcaaaagca aacatcgata cctgctctca gcaaaagatt caggccgtct
     5401 tcggcatggt agggctctcc gtagaccact cttactatac ctgcctgaat gattaatttg
     5461 gcacaatcta tacaaggaca ggcagtaaca taaatggttg ccccctggct gctgttgtgc
     5521 gaccgggcca ccttggttat ggcattggct tctgcgtgca aaacataagc ctttgtgttg
     5581 ccctcgctgt cttcgcatat attttcgaaa cccgaagggg tgccgttata accgtcggag
     5641 ataatacgtt tgtcttttac catcagagca ccaacctgtc tgcgcttaca gtaggaattt
     5701 tcggcccaga tcaaggccat acgcatatat cgtttatcga gtaaggcttg tttttcgttc
     5761 atttatttta ttccgttgcg tttaagtaaa gcatcaatac ggggttcacg acctctgaat
     5821 tttaaatata gttccatggg gtcttcggtg ccccctctgg aaagaatgtt ctcccggaac
     5881 gaagcagcac gctcagtatc gaagacatcg ttttcggtaa agtattcaaa ggcatcggca
     5941 tcgagtactt ctgcccattt atatccgtaa taaccggcgg catagcctcc ggagaaaata
     6001 tgattgaagc tggtgctcat gcaagcttcc ccgggttggg gaagcagaat aacagaactc
     6061 caggcttggt tttcgaattg acgtacgtcc ccgtcgaagg gtttttgaag accgtgccag
     6121 gccatgtcca aatagccgaa actcaactgg cgcaaacaag catatcctac gttgaaattg
     6181 tcggcagcaa ttattttttc gatcaattta gcaggaatct gttctccgct ctggtaatga
     6241 acggcgaaag tattcaggaa ttcttgctta gtgccccagt tttccattat ttgtgaaggt
     6301 agttccacaa agtcacggta aacattggtg cctgatagcg aggcataggt ggtattggag
     6361 agcattccgt gcaaagcgtg accaaattca tggaggaagg tagtcacttc atcaaaagtg
     6421 agcagagagg gcttttcgga ggtggaagga gtgaaattgg tgctcaatgt gatatggggc
     6481 cggatatctt tgtttttgat tttgtgttgt gctttaatat agctcatcca agctcccgag
     6541 cgcttgcttg ccctggggtg aaaatcggta taaagaacgg caaggaaaga accatccttg
     6601 tcgaaaacct cataggcagt cacctcctca tgccaaacct ggattttatt gtttttcttg
     6661 aaactgagac cgtataattt ggtagccagg ccgaacactc cttcgatgac attatccagc
     6721 ttgaaataag gtttgagcat ttcatcgttg agattaaact gcaggtcttt aagtttttgg
     6781 gaataatacg accaatccca ggcttccagt ttgatgaaat ttttctcgta tcccagggca
     6841 aatccttgaa gagccgaaag ttctctttgt cccagaggta aataggcttc cctgagctgg
     6901 tccagcaggc cgtaaacggt ttcggaattt ttggtcattc ttctgcgcag cacataggag
     6961 gcatagtcgg gatagccaaa taagccggcc atttcaaggc gggcattgac tatgtccctg
     7021 atgatgggct ggttgttgaa ttcaccatcg aggcctctgc ccatataggc ccggtacatt
     7081 tcttcgcgga gctctctgtt gtctgcgtat ttcagaaaag caccgtagct gggggcagta
     7141 aggtcaaaaa gccagccctc cagccctttg gctttggctt tttcggcagc tatgcttaac
     7201 acgtcttccg gaatgccgga caacaaggtt ttatcggtaa aaactttaga gtaggcattg
     7261 gtagctttca gcacattttg tccgaaattc agactgagtt ggctcagttt ggtgctcagt
     7321 tcccggtatt tttcacggtc ggcaccttcg agggtggctc cgctgtcgag gaagtccatg
     7381 taagtttccc agagtagccg gctttgttcc gtgtttaagt ttaggttttc cctgttgtcg
     7441 tatactgttt taattctctg atacagggca tcgttcagaa ctatgttgtt gtgatgctca
     7501 gataataggg gtgaaatttg ttgagataag ttcatcaact cgtcacaggt ctggttattg
     7561 agcaaagtgt aatagactgc cgatattttg tcgagcaatt caccggagcg ttccaacgcc
     7621 tcgatagtat tagcgaagga ggccggctcc ttattggcgg tgattttttg aatttcatta
     7681 tcgtgccggc gtatggcttc ctgataagca ggtagaatgt gttcaatttt aacttcccgg
     7741 aagggggctg tctcgtgcag ggttttgtat tttcccagaa aaggattgga accctgaaga
     7801 ccaataacgg tcagcgctgt cataataaaa aataatttag ttttcatctg ttcggttctt
     7861 tctgttttgt cctaattaat tcttttagta acagtatcat tgtgactgct tttgggatcg
     7921 cttataagca gtagaaaatg gaaagtaaaa tgagtttgtt gatgccggtt taagcaatca
     7981 tatccgaaat caagtcgagg taattgcata atacggtttg actgttgcgc atcaaaaaca
     8041 gtagagactt tctgtctatc tgcatgatgc tgcaattatc caaagctctg gcagttagcg
     8101 gcatattgcg tttccggccg aataaacgtg tatagacaat caattcaggt ccttgtgcga
     8161 tgcgtttttc cttttgttcg ttttcgagtt cgacttggcc gtccaataaa aagaccagat
     8221 tctcgcaaac gatgtctttg ttaaatataa gatctccggc agtatagttg tcgaagtcga
     8281 ggctgacttt ggttaaaagc gggaacaact catccatact cattccctta aacaaagggt
     8341 atttcaaaaa aagttcaaat ctggacatct cgcatttttt agagtacaaa caattccttt
     8401 gcgaaagtac tcaagatttc tgaaaatgcg ctatttttgc aaaaaataat aactatgaaa
     8461 atcaatttaa aagagatact aagcgctttc acggttttat ttgccatcat cgatattata
     8521 ggctcaattc caatcatcat tgatttccag aaaaaaccgg gaggagttcc cgccctaaaa
     8581 atttctttga tttcttttgc tcttttgatt ggttttttct tcctgggaga cggggtactt
     8641 gctttgtttg gagtaaatat atcttcgttc gccgtagccg gtgctatcgt gatttttgct
     8701 gtggcggtcg aaatgacttt cgatattgaa gtgtttcgcc acaaaggccc gcccgaagtt
     8761 tcttccatag tgcccttggt gtttcctctc attgtgggtg ccggatcttt cacaacgata
     8821 ctttcattaa aagctgaatt tcaagcgatt aatattatga tagcgctgtt cctgaacatg
     8881 gtggttgtct ttgtggtact caagggaatt aagcggattg aaaaatttat cggcaatgcg
     8941 ggtatttata tcgcccggaa attcttcggt atcattttgt tggccatttc tgtaaaatta
     9001 ttcacaacca atctgggtag cctgctcagc caattcaaat agaattggat gtgcacgata
     9061 tggaagatcc gggcgaatta gtgcctgcat agccgattca ggtagaatcc ggataatcag
     9121 tgcagcatta tgtttttaac agcctggctg cctatttttt cgttgaggcg tttaagcaac
     9181 tgaggcctga tcagcatcag ttcgttgcgg ataatggccg atttgacatg gacgtgcaaa
     9241 acggaatgtt tgaaatacaa attatccgta tgttcagcca gggctttgcc caccacttcg
     9301 ggccatagcc tgatagccgc cgtttcgtta agtttgcccc ggagttgctg gtttttgagc
     9361 acttcgtcca gtacttctgc cagacgttgg gtgtttttcc tgcgcataaa gctcttatta
     9421 tataggacaa atgcaggcgt tttctatgtg aaaaacgcga aaatccttac cgttgtcttg
     9481 taaaatcata ttcaggtgtt cccggtgggt atcggtaata aaaatttgtc cgaaatcttc
     9541 tttggaaact agttgaatga tgcgctctac cctcgaagcg tccagtttat cgaagaggtc
     9601 gtcaagtaga agtatgggtt ttttcttgcc ggcctgtttc aagtattcgt attgtgccag
     9661 ctttaaagca atcaggtaac ttttgttttg cccctgagag cctacccttt tgatgggata
     9721 gccatcgatt tgcatttcaa gctcgtcttt gtgaatgcct ttggtggtaa aacccagaat
     9781 aatatctttt tctctcgatc ggagaagttg ttcctgcaaa tttccctgct cgagatgcga
     9841 cctatattgc agtcctatac tctcgctgcg gtcggaaacg ctatggtaat aatgttcaaa
     9901 aaccggctga aaggcagcta taaattccct gcgcttttga taaatcaacc cggcataatc
     9961 gctcatggga gcatcgtaca tttcgtagag ggaatcctcc ttgttcatct gctcattttt
    10021 caacagggcg ttgcgttgat aaagtgcctt gttgtagctc ataagagcct ccatgtagga
    10081 gcgatcaaac tgcgaaaggg caatatccat aaacctccgg cgttcgtcac tgccttcgcg
    10141 aatcagattt tcgtcggcgg gagacacgat aactaggggt aagcgaccta tgtgatcggc
    10201 caggcgttcg tattcttttt tattgaattt gaatattttt ttttggcggc gcttcatggc
    10261 gcaataatac tcttcttctt tgcccccgat cagataatta ccctgcagca caaaatagtc
    10321 ggcttcgtga aaaatattct gggagtcgat cggattggta tgacttttac aaaaagaaag
    10381 ataataaatg gcatccagga aattggtttt gcccactccg ttattgccga aaaaacaatt
    10441 taaacgggga gaaaattcaa tattggcttc ttgtatattc ttataattga aaacggatag
    10501 cttttgtagg tacatggacg aatactggtc aaaatgcaca caaaagtata aaaaaaatca
    10561 cggaaatttt attaatagaa cgaaatgcta tacttttgca gaccaaattt gtctcataca
    10621 attacaaaag aaatggctaa gaaaaaagta tccaaaaaag cagctcaaaa ggaagaactg
    10681 caaaatgttg gcgaaatatt gacccgttcg gaacaattca tagaaaacaa ccaaaaaagc
    10741 cttttggtgg ctctgttggt gatcgtagtg gtggtaagcg gtatcattct tttcaggaac
    10801 agttatatgg gcccaaaaga aaaagaagct caggaaatga tgttccgcgg ggaacagtac
    10861 tttgctgcgg attcatttga gctggcttta tacggagacg attatgaatt tataggtttt
    10921 gaaggcatta tcgaaaatta cggcatcacc aaaactgcta aattggcctc tgcctatgcg
    10981 ggtttgtgct acaagcacct gggcgattac gaaaaagcca tcgacaggct caataaactc
    11041 aacgcggcag atattatggt ttcgcccgcg ttgacgggag caattggcga ttgttatgta
    11101 gaactcgatc aattacaaaa agccttgccc tatttcgaga gagcaggcca atccgacaac
    11161 gatttattag ctcccattta tttaatgaaa gccgggcgtg tttacgaagc attgggaaaa
    11221 tacgacagtg cccgcggagt ttacgaaaaa ataaaaaaga attatcctct ttcgcaggaa
    11281 ggtctgaatg tagacaggta tattgaaagg acaagattgc agaaaaaatg agcactaaaa
    11341 acctttccga atacaaccct gacactctgc ccgatgcgtc cgacatgaaa ataggcattg
    11401 tggtttccga atggaattac gatgttacgg ttaggttgtt ggaaggagca cgtcaatttt
    11461 tgctgcgtaa caatgtggat cctgaaaaca tccacgttgc gtgggttccg ggcagttttg
    11521 agttgattta cggggcagct aagatggagg cttctgaacc cgtggatgct gttatagctt
    11581 tgggtagtgt gatccgcgga gaaacgcctc atttcgacta tgtttgttct ggagtaacgc
    11641 agggtattgc acggctgaat gccaaaggga gcatcccctt tattttcggt ctgcttacca
    11701 ccgatgatat gcagcaagct ttaaacaggg ccggcggcat acatggcaat aaaggggtgg
    11761 aagcggctaa aactgccata cagatgattt acttcggtcg ccagctggat tatttggact
    11821 aaataagctt gccgcataat cccggcacat ttcttcaaac ttcagttatc ggataaaaag
    11881 tcgtttactg caactcaagg cttaattttt gtttcaaact ttgcaggttc ggattctttt
    11941 tgatcatttc gtccagctga tcctgagggc ttaagggctt ttttaactca tcctcttcgc
    12001 tgattctcaa cagcatgtga attttgccgt ttttcaaatc ccgttgcaaa ctactttcta
    12061 tatctccccg tatggcttca agtcgttggg cggtcagtgg gtgaaaaaca accacttcga
    12121 aatcgctttt gttcaacaaa cgaggcttgc aggcttgcat tgagtttgat aaataaggat
    12181 catccttgag tgtaccggca aaacgaagcc aggcttcaat aaggttttct tcgctgaaat
    12241 gctcttcagt cttaacagcc tcttttgtgg tcgcctcctg gcccgaagtg tcatctgctt
    12301 gaattttacg ccggcgtatg gaagtggtca gacccggtag tgacctttcc ttgcttgtcc
    12361 cggtgctggg attttgattt tgtggggcag gttcgggtac tgcggcttgc tgatgtgttt
    12421 gggcctcttt gttgccctcc acggtgttgc cgggttgagg attgggttgt ggttgtggtt
    12481 gtgattgtgg ttgtggtgtg gaatcagtta gtgttgcctg ctttgggttt gccgtaatag
    12541 atggagaaga ggggttagcc tgagtttggg ttttagaaga aggaagcgga tttttttcga
    12601 ctttggccgc cgcaatttta tccgttttca gttccccctg tttttttttt gcttcgccgg
    12661 tcagacaaag ttgaatcaga caaagttcca ccaaaagacg cttgtttttg ctttgattgt
    12721 attgcagact gcattcgttg ctgagtttga gggccttaaa caaaaagccc atgctgcatt
    12781 cttcggcctg ttttttatat ttgacgcgta cactctcagc ctgctccaag aggcttatgc
    12841 tgcggggatc ttttgagacc atgagatctc tgaaatgaga agccagccca ttgatgaagt
    12901 gcaggccgtc aaaaccctgg ttcaaaatct gatcaaacag caacaaagac tctgtagtat
    12961 tttcgtttaa aaaggcctcg accagcttaa aataatagtc gtaatccaat atatttagat
    13021 tatcgatgac gtgctgatag gtaattttgc cctcaccgta actggaaacc tggtcgaaaa
    13081 tggaaagggc atcgcgcatg cccccgtccg atttcagggc tatggcattc agggcttccg
    13141 gttccgtttc gataccttcg gagtcggcta cgtactgtaa ataagcacag atgtcgttga
    13201 tgccgatgcg atgaaaatca taaatttgac accgggagag tatggtgggt atgattttat
    13261 gcttttcggt agttgccaaa ataaaaatag catgggcagg tggctcctcc agcgttttga
    13321 gaaaggcgtt aaaagcgctg gaagagagca tgtgcacttc gtcgataatg t
//
LOCUS       DAOSGL010000019        13088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_3987_length_13088_cov_5.024937, whole genome shotgun sequence.
ACCESSION   DAOSGL010000019 DAOSGL010000000
VERSION     DAOSGL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_13088_cov_5.024937"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKY68_01360"
     CDS             complement(<1..714)
                     /locus_tag="PKY68_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOH23719.1"
                     /translation="MIDKRELEIIEFIKKNREVSSSEIFEGLSTSVSYATVKRRLSRL
                     IDENLLTKKGQGKSTKYLISPSYEILYPVDIEKYYEKEIDERDIRGSFNLQLIPKTLK
                     ECSVFTTNELVQLGLLQKKFEKNIAQLSETEYKKELERLAIDLSWKSSQIEGNTYSLL
                     ETERLLKEKETASGKTKEEAIMLLNHKEAIDFIIEYPDYLIPLSVPKIEDIHSLLIKE
                     LGVDKNIRKRRVGISGTNYR"
     gene            complement(805..2229)
                     /locus_tag="PKY68_01365"
     CDS             complement(805..2229)
                     /locus_tag="PKY68_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH23720.1"
                     /translation="MKKTLPLSLLLIVLFSCVNTKVETEQAQMDVEDCNCLENYHWIK
                     ETFENNDAGFEWVIQKKGMEAYQKFCDSIEAEIKNADDIHRCEEILNDWGKFFRKGHF
                     YVALNSSSSEDGGSVEAEKVSYSVETVEAQVEQTDDEIVGIWESSPYKIGIVLDTINP
                     KRKYVGFIIESGAPGWTKGDVKLEIFEQDNKLSSNYYMRDHSMEKRDVTLRNETELML
                     GSMRFINHSKIENEIERKMSDISEPFFYELSDYTTILKIPSFNLDQKPLIDKKIEQNK
                     EKILSHKNLIIDLRNNGGGGDNSWKELIPIIYTNPIKTVGVEYLSTGLNREYLLQNLS
                     FIQRIVLRGFIKKLKSNDGQFVVRDSVYTTELDEVLPNPQNIVVVVDDVCGSSVEQFL
                     LAAKQSEKVRIYGKQTYGALDVSNVTSVLSPDGCFRLGYCTTRTLRPEKDRIDDIGIK
                     PDVEINDSIPGHKWMEFILKEIDN"
     gene            complement(2275..2910)
                     /locus_tag="PKY68_01370"
     CDS             complement(2275..2910)
                     /locus_tag="PKY68_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23721.1"
                     /translation="MSDKVKKNSIIFSPFNLIDPINPSFQVGYQRLLYDKIELQMEYG
                     HIINKALFHYIINPDEGKEEYSNKGFKVRFETKRFFRNEGFFRYYLSGELFYLQNVSK
                     VQNQFLVSDPDYNYSFDLPNDGEQYGYTDYFTNSKTKYGFNVKFGIKLVIDPLFFEAC
                     NGIGIAYRNNIHSDRENMNDEPYDTSFLNDNIPKKMYILNLPLNFRIGYMF"
     gene            complement(3004..3630)
                     /locus_tag="PKY68_01375"
     CDS             complement(3004..3630)
                     /locus_tag="PKY68_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23722.1"
                     /translation="MNFKTEQEKFWAGDFGNEYVVRNSDPNSIAQRTAVFSKILSRTK
                     DVNSFLEFGSNIGLNLIALRNLIPSCSLSAIEINSKAIEVLKNIPGTKIFEGSFLNFS
                     SNELDKHDLTFTSGVLIHVNPGKLREVYSKLYECSNKYILVIEYYNPKPVEVNYRGNS
                     ERLFKRDFAGEILDLYPDLELVDYGFQYHRDNNFPMDDLTWFLLKKNK"
     gene            complement(3833..4594)
                     /locus_tag="PKY68_01380"
     CDS             complement(3833..4594)
                     /locus_tag="PKY68_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23723.1"
                     /translation="MKIRTEILIGLLIFIISCDTGTGKKSNIRKDDSQENVKTDNRKL
                     AFQNNELISCYDFSTGETKNLTEGFDPCISPDGEWIVFTQSSNVGDNFSRTIKLINTE
                     NSTIKDLEINNNNHYGAIWSPTGEHLAFSIMTNDWQIGLIKPNGTELKIISTRFCFTT
                     DNKKMVFDGGIDEFIEGLNEPLCAIFCYDFNTKSTKRISKKGLYAMDLWMDNQDRIYF
                     SGFENINEPGKIYQTSLTDTTLIELIEGTRPSMGG"
     gene            complement(4654..5622)
                     /locus_tag="PKY68_01385"
     CDS             complement(4654..5622)
                     /locus_tag="PKY68_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOH23724.1"
                     /translation="MNINRLIHVIMFYRLKNPAKIAICAFILFFVKYAEAQNLTLIAP
                     AEPNAIPLGTGGVDNQSASESWYSQGGDPMVRNVSTATLTPFLPEPGKANGAAVIVAP
                     GGGFTCLSMIREGWEVAEALAKQGIAAFVLKYRLNPTPASLEEYKAVTSRAFTNPTKS
                     PDSTKEVVPARPMRFDLSNQLKDADAAYTMIVRRAEDWGVDTTRIGMIGFSAGAALTM
                     HSTLNSKIMHLAFIGPIYGGMGSVEVPKDAPPMFCVIAADDFLFRGNFGVIDSWYKAG
                     IPVEFHLYQNGGHGFGLGKPNRTSNRWFDAFIYWLDVNGFLSAKSE"
     gene            complement(5876..6922)
                     /locus_tag="PKY68_01390"
     CDS             complement(5876..6922)
                     /locus_tag="PKY68_01390"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOH23725.1"
                     /translation="MNNKLSILFGLSLMLLTSCSSGYTIKGKLAGNELDGQQVMIINL
                     ATDEAVDSGLVESNSFLIKGTVEEPAVCAALIGNKFSYFVLENARIALDMEKSFNASA
                     TPLGKSFEALYTAMNQLNESTNQKLGELAPEEQQAYYENEVIPASKVLAKEHLDKNLD
                     NTVGEFAYMLYSSFADPQELIDAYEAMGDNLKDNPQTLRIYQKSKSMLTTAEGMPFVD
                     FTVTNAQGTQSLSDYVGKGRYVLVDFWASWCGPCLLETPNIVELYEKYHKKGLDILGV
                     AVWDKPEDTQKKIEELGLPWPQIIDAQRIPTDIYGIQGIPHIILFGPDGTIVARNLRG
                     EAMKAKIAEVMGAK"
     gene            complement(6988..7995)
                     /locus_tag="PKY68_01395"
     CDS             complement(6988..7995)
                     /locus_tag="PKY68_01395"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOH23726.1"
                     /translation="MKKLALLVFLFLLLSCSGKKGNDSLQDGRQTSPVHSRKASQNVE
                     NRPAGRTEAPKSFTMPEIPPMLVESGSRTLYLLNHFWDNFDFSDTSYIGLKEITERAF
                     IHYAQMLSQRDVVPYDKAVEGMLIMLNKAQADSSMYRYFTGLCKKYFYRGNSPMMNDE
                     YYIPVLEHMLQSPHLAPASKKAAELDLKRLKRNLPGQSAENFDYTLHSGKTQTLYQLK
                     AEYILLFFNNPDCPACKEVQEQISASPLLSKLIQKGSIKVLALYPDSDLAAWDKHREQ
                     IPSAWINAYDKDQRITLDELYDLKAIPMLYLLDGQKKVLIRDGKVKQVEQLLSAIIQQ
                     E"
     gene            complement(8088..9470)
                     /gene="glmM"
                     /locus_tag="PKY68_01400"
     CDS             complement(8088..9470)
                     /gene="glmM"
                     /locus_tag="PKY68_01400"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOH23727.1"
                     /translation="MNQLIKSISGIRGVIGGTPENSLNPFNIVRFTAAYARWIRQNSD
                     RQSGKIVVGRDARISGEMLFQLVSGTLMSLGFDVLDIGLATTPTTELAVRMSGADGGI
                     ILTASHNPKQWNALKLLNQEGEFLDAAAGRFVCDQADSDRVEFAGVDQLGKIYREDFT
                     QKHIEAVLALPLVDVEAIYKAGFRVAIDCVNSVGGLVLPPLLRALGVQEIIELNCTPD
                     GHFPHNPEPLPQHLGEISSLLSRGGIDVGFVVDPDVDRLAIVCEDGSMFGEEYTLVAV
                     ADYILSRTPGNTVSNLSSTRALRDVTEKYGGKYFSAAVGELNVVAQMKAVNAVIGGEG
                     NGGVIYSESHYGRDALVGIALFLSHLAHKQQKVSELRAELPAYFMDKQRLDLSPGLSL
                     PLIFDTLKKRYAGEKITDIDGVKIDFSDRWVHVRGSNTEPILRIYAEAPSQEAANALA
                     TEVMELIHRL"
     gene            complement(9479..10048)
                     /locus_tag="PKY68_01405"
     CDS             complement(9479..10048)
                     /locus_tag="PKY68_01405"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HOH23728.1"
                     /translation="MKCPILPCFLLAVALLSASCNKDVITYAEQLEAQQKSISKFIDY
                     NGFLVIDSIPSNVPWEDNEGRKMFYLSKSGLYLHVVDTGRQAGKTVNNTLVCVRYTEC
                     SMEGDTTYSNMDYATDPIEIYFNTVSTGTGSSTYYWGDCKAWHEPLNYVGDLGHVMII
                     APASVGMPYYSAGNRMLARYYDLRYTFWK"
     gene            complement(10089..11135)
                     /locus_tag="PKY68_01410"
     CDS             complement(10089..11135)
                     /locus_tag="PKY68_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH23729.1"
                     /translation="MISKIIDEDLIQKTNSLLQKCDNIVLITHEYPDGDGLGSALAMY
                     DYLSLEGKQVSVLLPNEIPSFLQWMPGADSVIVYEQDPETARSLLGQAEVIIFLDLNS
                     MERVGAMASFISQLTVHTVLIDHHNLREKSIAHVEIQLPQIASTCELVFRYICRVGAF
                     EKLSKDCAVCIYTGMMTDTGAFTYNSQSNEMYLIINQLIVKGIDKDEIYKKVYGNYTE
                     NRMRLMGYALYEKMEVLPVYQTAIITLSCEELDRFGHQAGDTEGLVNQPLNIGNVRFS
                     VFLREEEQNEIKISLRSEGDLSVSKIAADLFQGGGHFNASGATYRGSMQEALRIIKEA
                     LPNYLGTPEDEEDN"
     gene            complement(11140..12765)
                     /locus_tag="PKY68_01415"
     CDS             complement(11140..12765)
                     /locus_tag="PKY68_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOH23730.1"
                     /translation="MLQVFSYGMVSFVFQQAPFLRITISLISGMILAPFEFFPGLLFS
                     FILLLATVFLRLFTAKAGAAWRLRKLPGFLILLCCFCLGSALFRGRQQERACPKLENY
                     YLRAEFEILSNPQIKPNSLQCPARIICSEPSCWKNKRCVLYLAKDSLASSLKAGNRIL
                     AQTTLKPYSRCGRPMEFNYERYLLNKGFCAGAFVPARAWMLQSRYSSPRAKSDAFRDR
                     LIGRFRQAGMEEEPLALVSALVLGCKDLMEAEQRQAFSSAGVAHILAVSGMHVSILFL
                     LLDKLLFFIKPCRAGLFFKQSLLIVLLWFYAFVTGMSASVLRAVLMCNVSALGLCLGR
                     RSNSWNTLAFSAFALLTVNPCYLTDVGFQLSFMAVASILLLMPGAEYYLGMITERSAS
                     VAKKSIKAKIVGIYRETLLLSLVAQLGTAPLSIYYFNQFPQYFLLGNVLLVPLATLLF
                     YLCFAFIVLSPLPWLSEVLVWPVNVLASFFCRSSAYIENMRGSLVTDLWPSKQDVLIC
                     YLLLALLFYLFKNKRYICALNKLISKLYKQISD"
     gene            complement(12822..>13088)
                     /locus_tag="PKY68_01420"
     CDS             complement(12822..>13088)
                     /locus_tag="PKY68_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOH23731.1"
                     /translation="DLDLVLLMSVNPGFGGQVFIKQTYAKIAKLRQMIDDCKSPALIQ
                     IDGGVSLDNAGALYRAGADVLVSGSFIFKSPRPKEIIEQIKKAE"
BASE COUNT         3635 a         2970 c         2629 g         3854 t
ORIGIN      
        1 gcgataattt gttcccgaaa tacctactct tctttttctt atgttcttat caaccccgag
       61 ttcttttatc agcaaactat gtatgtcttc aattttcgga acggataatg gaatcaggta
      121 atcagggtat tcaattataa agtcaatggc ttccttgtga tttaaaagca taattgcttc
      181 ttcttttgtt ttccctgatg cggtttcctt ttctttaagt aatctctccg tttccaaaag
      241 agaataagta ttcccttcaa tctgcgacga cttccagctt aaatcaattg ccaatctctc
      301 caattccttt ttgtattctg tttccgatag ttgtgcaata ttcttttcga atttcttttg
      361 aagtaagcct aattgtacta attcattggt tgtaaacacg ctacactctt taagtgtttt
      421 gggtatgagt tgtaaattaa aacttcccct aatatctctt tcatcaattt ccttctcata
      481 atatttttca atgtcaacag ggtaaagaat ttcatagctt ggtgaaatca agtatttggt
      541 tgatttccct tgcccctttt ttgttaagag attttcatcg atcagcctgg aaagtcttcg
      601 ttttacggta gcgtaactta cagatgttga aagaccttca aatatttcac tcgaagaaac
      661 ttcccggttc ttctttatga attcaattat ttctaattct cttttatcaa tcatagagct
      721 tcgatttaag gctcacaaac atacttattc cggctcaaat ggtcgtaata atgagctgca
      781 tttcttttta aaatgagcta caaattagtt atcgatctct ttcaaaataa actccatcca
      841 tttatgtccg ggtatggaat cgtttatttc aacatcgggt ttaattccga tgtcgtctat
      901 ccggtctttt tcggggcgta gagttctggt tgtgcaatag cctaatcgaa agcaaccatc
      961 gggagacaat acactcgtta cattggaaac atccaaggct ccataagttt gctttccata
     1021 aatcctgact ttttcacttt gttttgccgc caatagaaat tgttcaacag aacttccgca
     1081 aacatcgtca acaacgacaa caatgttttg aggattgggt aaaacttcat ctaattccgt
     1141 tgtgtaaaca ctgtctctta ctacaaattg tccatcgttg gattttaatt ttttaataaa
     1201 accccttaat actattcttt gaataaatga gaggttttgc agtaaatatt ctctgttcaa
     1261 cccggttgat aaatattcca cccctaccgt ttttatcgga ttggtataga tgattggaat
     1321 caactctttc caggaattat cgccgccacc accgttatta cgcaaatcga tgatcagatt
     1381 tttgtgcgac aatattttct ccttgttttg ttcaattttt ttgtctatca acggtttttg
     1441 gtccaaattg aatgaaggaa ttttcaatat cgttgtgtaa tccgacaatt cgtaaaaaaa
     1501 tggttccgaa atatccgaca ttttcctttc tatctcattt tcaatttttg aatgattgat
     1561 aaacctcatg gagcccagca ttaattccgt ttcatttctc aacgttacat ctctcttttc
     1621 catcgaatgg tctcgcatat aataattgga agacagtttg ttatcctgtt cgaagatttc
     1681 aagtttaacg tctcctttgg tccagccggg agctcccgat tctattataa aacccacgta
     1741 tttccgcttt ggatttatgg tgtcgagcac aatgccgatt ttataaggcg acgattccca
     1801 aatgcctact atctcatcgt ctgtttgctc aacttgtgcc tctacggttt ctactgaata
     1861 actgactttt tccgcttcaa cagaacctcc atcttcggat gaagacgaat ttaaagccac
     1921 ataaaagtgt cctttgcgga aaaactttcc ccaatcgttt aaaatctctt cgcatctgtg
     1981 aatgtcgtcg gcgtttttta tttccgcttc aatggaatcg cagaactttt gataggcctc
     2041 cattcctttc ttctgaatta cccattcaaa tccggcatcg ttgttttcga atgtttcttt
     2101 tatccaatga taattctcca ggcaattaca atcttcaacg tccatttgcg cttgctctgt
     2161 ttctactttg gtgttgacgc acgaaaatag aacaatcaat aataaactta gggggagtgt
     2221 ttttttcatt gttgttttac cggtttatcc tgagtattgg ctccggttct tttattaaaa
     2281 catatatcct attctgaaat tcagtggtaa attaagtata tacatcttct taggaatatt
     2341 atcgtttaga aaactcgtgt cgtatggttc atcgttcata ttttctctat cactgtgtat
     2401 attattccga taggctatcc ctataccatt acaagcctca aaaaacaaag gatcgattac
     2461 gagtttgata ccaaatttta cgttaaatcc gtactttgtt ttagaatttg taaaataatc
     2521 ggtgtatccg tattgctccc catcgtttgg taaatcaaat gaataattat aatcagggtc
     2581 tgaaactaaa aattgattct gtacttttga aacattttgc aaatagaata attcaccgga
     2641 cagataatac ctgaaaaaac cttcatttct aaaaaacctc ttggtctcaa atcttacttt
     2701 aaatcctttg ttagaatact cctctttgcc ttcgtccgga ttgataatat agtgaaacaa
     2761 agccttgttt atgatatgtc cgtactccat ttgcagctca attttgtcgt ataataacct
     2821 ttgataaccc acctgaaaag agggatttat cggatcaatc agattaaaag gagagaaaat
     2881 aatcgaattt ttcttcactt tatccgacaa cttgagggca tcttgcccaa aagaatttaa
     2941 tgtgaaaaag cagactgaaa aacagactaa tattcgtgtg aacaccatat tattatgggc
     3001 cggttattta tttttcttga gcaaaaacca agttaaatcg tccattggga aattattgtc
     3061 tctgtgatat tgaaaaccgt aatcgaccaa ttctaaatcg ggatataagt ctaaaatctc
     3121 accggcaaaa tctcttttga aaagtctttc ggaatttccc cggtaattca cttcaacagg
     3181 tttggggtta taatattcta ttacgagaat gtatttattt gaacattcat atagttttga
     3241 atacacctcc cgcaatttac cgggattaac atggatcagt acaccggatg taaacgtcaa
     3301 atcgtgtttg tctaattcat ttgaagaaaa atttaaaaat gaaccttcaa atattttcgt
     3361 acctggaata ttttttaata cctctatggc ttttgaatta atttcgatcg cgctcagcga
     3421 acacgatgga attaagtttc ttaacgctat caaattcagc ccaatattgg agccaaactc
     3481 aagaaaggaa ttcacatcct ttgttcttga taaaattttt gaaaatacgg cggtcctttg
     3541 ggctatgcta ttcggatcgc tatttctaac aacatattca ttgccaaagt cacctgccca
     3601 aaatttttct tgttctgttt taaaattcat attgctcttt attttccctt actcgtttag
     3661 cttttcaatt tcgccctatt tttgttacgg tctatatcta tcgtattgaa aacgttctat
     3721 ctaattcaat aaatcaccac aaatttaatc atttcccgac aaaacatctt tcttgcagcg
     3781 gttttctcaa aagacataca aaattttatg gtatcgtgtc gtgcgccata ttttatcctc
     3841 ccatactcgg tctcgttcct tcaattaatt ctatcaaagt cgtatctgtt aaacttgttt
     3901 gataaatttt tcccggttca tttatatttt caaatccgga aaaataaatc ctatcctggt
     3961 tgtccatcca caagtccatt gcatacaatc cttttttaga aatccgtttc gtagattttg
     4021 tgttaaaatc atagcaaaaa atggcacata acggttcatt caatccttcg ataaactcgt
     4081 ctattccgcc gtcaaaaacc atttttttat tatcggtcgt gaagcagaat cgtgttgaga
     4141 tgattttcaa ttcggtacca ttgggtttta tcagtccaat ttgccaatca ttggtcatta
     4201 tactaaatgc cagatgttct ccggttggag accatattgc tccgtaatgg ttgttgttgt
     4261 tgatttctaa atcctttatt gttgagtttt ctgtattgat tagtttaata gtccttgaaa
     4321 aattatcccc tacgtttgat gattgtgtga aaactatcca ttccccgtcg ggcgaaatac
     4381 atgggtcaaa tccctctgtt aaatttttag tctctcccgt tgaaaaatcg taacagctta
     4441 tgagttcatt gttttgaaaa gcaagtttcc tgttgtccgt tttcacattc tcttgtgaat
     4501 catctttccg tatgtttgat ttcttacccg ttccggtatc gcacgaaata ataaaaatca
     4561 aaagcccaat taaaatttcg gtcctgattt tcatgatttt taaattttag aagttgatac
     4621 tcgcttgttc tataatgttt taagctctta tcattattca gatttagcag aaagaaagcc
     4681 attaacatct aaccagtata taaaggcatc aaaccaacga ttacttgttc gattaggctt
     4741 tcccaaccca aaaccgtggc ccccattttg gtaaagatgg aattcaacgg gtattccggc
     4801 tttgtaccag gaatcgataa caccaaaatt acccctaaag agaaaatcgt cggccgctat
     4861 aacacagaac ataggaggag catcctttgg tacttctacc gaacccatac caccatagat
     4921 cggaccgatg aaagccaggt gcatgatttt ggagttcaat gtggagtgca ttgtgagggc
     4981 cgccccggct gaaaagccaa tcatacctat tctggttgta tcaacacccc aatcttcagc
     5041 tcgtctgacg atcatagtat aagcggcgtc agcatccttg agctgattgg aaaggtcaaa
     5101 gcgcattggt cgtgccggca ccacttcttt agtagaatct ggggattttg ttggatttgt
     5161 gaatgcacgg ctcgtcacgg ctttatattc ctcgagcgat gccggagttg gattgagtct
     5221 gtacttaagt acgaaagcgg ctattccctg ttttgccaga gcttctgcta cctcccatcc
     5281 ttccctgatc atcgaaagac atgtaaaacc tcctccggga gctacaatca ccgcagcacc
     5341 atttgcttta ccgggttccg gtagaaatgg tgtaagggtc gcagtagaga cgttacgaac
     5401 catcgggtcg cccccctggc tgtaccagga ttctgaggcg gattgattgt cgactccacc
     5461 tgtgcctagc ggaatagcgt ttggttcagc cggagctata agtgttaggt tttgggcctc
     5521 tgcatactta acaaagaaaa gtatgaaagc acaaatggct attttagccg gatttttaag
     5581 tctataaaac ataattacat gaattaatcg gttaatattc aaatgtaatg cttttacaat
     5641 tgttctgaaa tatgcctttc aatcacatgc aagttgcaac aataccacat aatgcctgat
     5701 aaacttattg ggtttatttc cactctacac aaaccctcaa cagccgaaaa aagcgaccgc
     5761 aatagtccgt caaaaaccga agatagacca gacataattg ggtgatttgt gttttatggt
     5821 attcattatc cgttagttaa tcccgacaca gataaaaccg tgttgagaga aggctttatt
     5881 ttgcccccat cacttcggca attttggctt tcatggcttc accccgaagg ttgcgagcaa
     5941 caatggtgcc gtcggggcca aacaaaatga tgtgcggaat gccctgaata ccgtaaatat
     6001 cggtgggaat acgctgtgca tcaataatct ggggccaggg aagacccaat tcttctatct
     6061 tcttttgagt atcttcgggt ttatcccaaa cagctacacc caaaatatcg aggccttttt
     6121 tgtggtactt ttcgtacagc tccacgatat taggcgtttc cagcaaacaa ggcccgcacc
     6181 aactggccca gaaatcgacc aacacatacc tgcctttgcc tacgtaatcc gaaagcgact
     6241 gtgtgccctg ggcattggta acggtgaaat ctacaaaggg catgccttcg gccgtggtca
     6301 acatcgattt actcttttgg taaatgcgca gagtctgagg attatcttta agattgtcgc
     6361 ccatagcctc ataggcatcg atcaattcct gaggatcggc aaacgaagaa tacaacatat
     6421 aggcaaattc acccacagta ttgtctaagt tcttatctaa gtgttctttg gccaagacct
     6481 tagaagccgg aataacctcg ttttcgtaat aagcctgttg ctcttcggga gcaagttccc
     6541 ctaacttttg gttggtagac tcgttgagct gattcatggc cgtataaagc gcctcaaaag
     6601 atttgcccaa aggagtagcc gaagcattga aagacttctc catatccaga gcaatacgag
     6661 cattttcgag cacgaaatag gaaaatttat tgccgataag agcagcacat accgccggtt
     6721 cttctacagt tcctttgatc aggaagctgt tgctttcaac caacccgctg tccaccgctt
     6781 cgtcggtggc gagattgata atcatcactt gctgtccgtc caactcgttg ccggccagtt
     6841 tgcccttgat ggtatagccg gagctacagg atgtgagcag catcaggctc aggccaaata
     6901 aaatactcag tttgttgttc atgcttgatt gtttttaatt ggttgtttta aaataattat
     6961 taatgaattg ctattcctgg ataatcgcta ctcttgttga atgatagcgg ataaaagctg
     7021 ttccacctgt ttcactttgc cgtctctgat caggactttt ttttggccgt ccagcaaata
     7081 gagcatggga atggccttga gatcgtataa ttcgtcgagt gtgatccgtt gatctttgtc
     7141 gtaagcgttg atccaggcag agggaatctg ctcccggtgt ttatcccagg cggccagatc
     7201 gctgtcgggg tatagagcca atactttgat gctgcccttt tggataagct ttgacaagag
     7261 cggcgaagcc gaaatttgtt cctgcacctc tttgcaggcc gggcaatcgg ggttattgaa
     7321 aaacagcagg atatactcag cctttagttg gtataaagtt tgagttttac ccgaatgcaa
     7381 agtgtagtcg aaattttcgg ccgattgtcc cggcaaattg cgtttgaggc gttttaaatc
     7441 gagctcggca gccttttttg aagcgggagc caaatgagga ctttgcaaca tatgttccaa
     7501 tacgggaatg tagtattcat cgttcatcat gggcgaattg ccccggtaaa aatatttttt
     7561 gcagagccct gtaaaataac ggtacatgga gctgtccgcc tgagccttgt tcaacataat
     7621 caacatgcct tcgactgctt tatcgtaagg aacaacatcg cgctgactga gcatttgggc
     7681 ataatggata aaggcccgtt cggtgatctc ttttaaaccg atgtaagaag tgtcgctaaa
     7741 atcaaaattg tcccaaaagt gattcagcag atacaaggtc cggctgccgg attccaccag
     7801 catgggcggt atctcgggca ttgtaaaact tttaggagct tctgtccgac ctgcaggccg
     7861 gttttcaacg ttttggcttg ccttgcggga atgcaccggc gaagtttgcc gaccgtcttg
     7921 caatgaatca ttgccttttt tgccgctgca cgacagcaac aaaaagagga aaacgagcaa
     7981 tgcaagcttt ttcattgttc cagacattaa ttgttacggg cattatagta cctccaccgg
     8041 cttttcatcc ggcccccgcc atcgaaatgg gcacaaaacg ctccacttta gaggcggtgt
     8101 atcagctcca tgacttccgt cgccagagca ttggctgctt cctgagaggg agcttcggcg
     8161 taaatgcgca gaatcggttc ggtgttggaa cctctcacat gcacccagcg gtcgctgaaa
     8221 tctattttga ccccgtcgat gtcggtaatc ttctcgccgg catagcgttt tttgagtgta
     8281 tcaaaaatca ggggcaagct caggccggga ctcaaatcga gtcgttgttt atccatgaaa
     8341 taagccggca attccgcccg cagttcggat actttttgct gtttgtgagc caaatgcgaa
     8401 aggaacaagg caatgcccac cagggcatct ctaccgtaat gcgattcgga ataaatcact
     8461 cccccattgc cttcgccccc tataacggca ttaacggctt tcatttgggc gaccacgttc
     8521 aattctccca cggcagcgga aaaatacttg ccgccgtact tttcggtcac atcgcgtaag
     8581 gcgcgggtcg aactcagatt ggaaacggta ttgcccggcg tacggctcag aatgtaatcg
     8641 gctacggcaa ccagagtgta ttcttcgccg aacatggagc cgtcttcgca aacaatggcc
     8701 aaacgatcca cgtcaggatc gaccacgaag cccacgtcga taccgccccg gctgagcagg
     8761 gaggaaatct cgcccagatg ttggggcaag ggttcgggat tatgcgggaa atgcccgtcg
     8821 ggagtacaat tcagttcaat gatttcctgt acgcctaagg ccctgagcaa aggaggcaac
     8881 acaaggccac ccaccgaatt gacacaatca atggcaacac ggaacccggc tttataaata
     8941 gcttcaacat ccactaaagg caaggccagc acggcttcga tatgtttttg agtaaagtct
     9001 tcgcgataaa ttttgcccaa ttggtctact ccggcaaatt ccacccggtc gctgtcggcc
     9061 tggtcacaaa caaaacgacc ggcagccgcg tcgagaaatt caccctcctg attgagcagt
     9121 ttcagggcat tccactgttt tgggttgtga ctggctgtaa gaataatacc tccatcggct
     9181 cccgacatcc gtaccgccag ctcggtggta ggcgtggtag ccaaaccgat atcgagcaca
     9241 tcgaaaccca gactcatcaa ggttcccgag accagttgaa aaagcatttc gcccgagatg
     9301 cgggcatcgc gtcccacaac tatttttccg gattgcctgt cggaattctg acgaatccag
     9361 cgcgcataag cagccgtaaa ccgaacgata ttaaaaggat tcaggctatt ctccggcgtt
     9421 cctcctatca cacccctgat acccgaaata gatttaatta attgattcat aaaattattt
     9481 acttccaaaa agtatatctt agatcgtaat acctcgccaa cattctattg ccggccgaat
     9541 aataaggcat acctacactg gccggcgcta ttatcattac atgccccagg tcgccgacat
     9601 agttcagagg ttcgtgccag gctttgcaat cgccccagta gtaggtactg gaaccggtac
     9661 cggtggatac ggtgttgaaa taaatttcga tgggatcggt ggcgtagtcc atattggagt
     9721 aggtagtgtc gccctccatg gaacattctg tataacgtac gcaaaccaac gtattattga
     9781 ctgttttacc tgcctgccta ccggtatcca ccacgtggag atagagtccg cttttgctca
     9841 gataaaacat tttacgacct tcgttgtctt cccagggcac attggaggga atggagtcga
     9901 tcactaaaaa tccattgtag tctataaatt tgcttatgct cttctgttgg gcttccaatt
     9961 gttcagcata ggtaatgacg tccttgttgc aggaggccga caacagggca acagccagca
    10021 aaaaacaggg taaaatgggg catttcatac taaatccgtt gaataagagc gcaaaggtaa
    10081 gttttttttt aattatcttc ctcatcttcc ggagttccca ggtagttggg caaagcttct
    10141 ttgattattc gcagggcttc ctgcatactg ccccggtaag tggcgccgga agcattgaaa
    10201 tggccgcccc cctgaaagag atccgcagct atttttgaga cggacagatc gccctcggaa
    10261 cgcaaggaaa ttttgatttc gttctgttct tcttcgcgta aaaacactga aaagcgcaca
    10321 ttacctatat taagcggctg atttacaaga ccttcggtat cgccggcctg atgaccaaaa
    10381 cgatccaact cttcgcaact cagcgttata atggctgttt gatatacagg caggacttcc
    10441 attttttcgt agagcgcgta gcccatcagg cgcatgcgat tttcggtgta attgccgtag
    10501 acttttttat aaatttcgtc cttgtcgatg ccttttacaa ttaattgatt tataatcaga
    10561 tacatctcat tggattgaga attgtaggta aaagctcccg tatctgtcat cattcctgta
    10621 taaatgcaga ctgcgcaatc tttgctcagt ttttcgaaag cccccaccct gcagatgtag
    10681 cgaaacacca actcacaggt tgaagctatt tggggcaact gtatttccac atgcgcaatt
    10741 gacttttcgc ggaggttgtg gtgatcgatc aacaccgtgt gtaccgtcag ttgactgata
    10801 aaggaagcca tggctccaac tctttccatg ctgttgagat ccaggaatat tatcacttct
    10861 gcttgtccga gcagactgcg tgcagtttcg ggatcctgtt catacactat tactgaatcg
    10921 gcaccgggca tccattgcaa aaaagaggga atttcgttgg gcagcagcac actgacttgt
    10981 tttccctcca aacttaaata atcgtacatg gccaaagctg aacccaggcc gtcgccatcg
    11041 gggtattcgt gcgtaatcag cactatattg tcgcattttt gcaataagct atttgtcttt
    11101 tgaatcaggt cctcgtcaat aatcttcgat atcatagttt taatcggaaa tttgcttata
    11161 caacttagat ataagcttgt tgagggcgca aatataccgc ttatttttga acagataaaa
    11221 aagcagggcc aaaagcagat aacaaatcag cacatcctgt tttgaaggcc aaagatcggt
    11281 aaccagcgaa ccgcgcatat tttctatata agcgctgctg cggcaaaaaa agcttgcaag
    11341 tacatttaca ggccagacca gaacctcgct caaccaaggt aaaggactta aaacaatgaa
    11401 agcgaaacag agataaaaca ggagggtggc caggggaacc aacaaaacat tgcccaacaa
    11461 aaaatactga ggaaattggt taaaataata aatagaaagc ggagcggttc caagctgggc
    11521 aaccaaagac agcagcaggg tttcgcgata aatcccaacg attttggcct ttatgctttt
    11581 tttggcaacc gaggcagacc gctccgtaat catgcccaaa taatactcgg cacccggcat
    11641 taataacaga atggaagcaa cagccataaa actcagctga aaaccgacgt ccgttaaata
    11701 gcagggattg actgtcaaga gtgcaaaagc cgagaaagcc agcgtattcc agctgtttga
    11761 acgtcgtccc aaacaaagcc ccaaagcaga aacattacac atcagcactg ccctgagtac
    11821 cgacgcagac atgcccgtaa caaaggcata aaaccaaagc agaacaatga gcaggctttg
    11881 tttgaaaaag aggccggcac ggcacggctt aataaaaaaa agcaacttgt cgagcagcag
    11941 aaacaaaata gacacatgca ttccgcttac ggccagaata tgggctacgc ctgccgaaga
    12001 aaaagcctgc ctttgctctg cctccatcag gtctttgcaa cccaacacca gtgccgaaac
    12061 caaagccagg ggctcctctt ccattcctgc ttgtcgaaaa cggccgatga ggcgatcgcg
    12121 gaaagcatcc gattttgccc gcgggctaga gtatctactc tgcagcatcc aggcccgggc
    12181 aggcacaaaa gctcccgcac aaaagccttt attcaacaaa tatcgctcat aattaaattc
    12241 cataggcctg ccgcaccgac tgtatggctt caaggtggtc tgtgccaaaa tacggttgcc
    12301 ggccttcaac gacgaagcca gactgtcttt ggccaggtaa agcacacaac gtttattttt
    12361 ccagcaggaa ggctcggagc aaattatcct ggcagggcat tgcagactgt tgggcttaat
    12421 ttgaggattg gacaaaattt cgaattcagc cctcaggtaa taattttcga gcttcggaca
    12481 ggccctttcc tgttgtctgc cccgaaaaag agcagaacct aaacaaaagc agcacaacaa
    12541 aatcaagaag ccgggcagtt tgcgcaaacg ccaagccgct ccggctttag cggtaaacag
    12601 ccggagaaac acagtcgcca gcagcaagat aaaggaaaac aacaagcccg gaaaaaactc
    12661 aaaaggcgca aggatcattc ccgatatcag ggaaatcgta atgcgcagaa agggtgcttg
    12721 ttgaaaaaca aaagatacca tgccgtaaga gaatacttgc agcaaagata tttatttatt
    12781 aatgtttcgg aaacctttaa gagcttttat ttcaaactct cttactcagc tttttttatt
    12841 tgctcgatga tctccttggg ccggggcgac ttgaaaataa aactgcccga caccaacaca
    12901 tcagcgcctg cacgatacaa agccccggcg ttatcgaggc tgacacctcc gtcgatctgg
    12961 atgagagccg gactcttgca gtcatcgatc atttgcctga gcttagcgat ttttgcataa
    13021 gtctgcttta taaagacttg gccaccgaaa ccgggattga ccgacataag cagaaccagg
    13081 tccagatc
//
LOCUS       DAOSGL010000020        12878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4068_length_12878_cov_4.415971, whole genome shotgun sequence.
ACCESSION   DAOSGL010000020 DAOSGL010000000
VERSION     DAOSGL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12878
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4068_length_12878_cov_4.415971"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PKY68_01425"
     CDS             complement(<1..983)
                     /locus_tag="PKY68_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HOH23732.1"
                     /translation="MKNALFVASIVFLLSFTLQLSAQNNRFDPEGSSTPSVHDPVVAK
                     QGNTYYLFCTGFGISVLSSTDMINWKREKPVFSEPPAWAVEAVPGYRGHTWAPDIIKH
                     QGKYYLYYSVSTFGKNNSCIGVATNKTLDPGSPDFKWEDQGMVLRSIPNRDMWNAIDP
                     NIIIDEDGTGWMSFGSFWEGIKLVKLDSSLCALAEPQEWYPLSRRPRTLNDIDVEPGG
                     GAVEAPFIFRKGSFYYLFVSFDMCCRGAQSTYNVVVGRSRKVTGPYVDREGNVMTKGA
                     GTLVVKGNERFPGVGHNAAAGFDGKGYLFFHGYGMERNGRSILLIREIKWD"
     gene            1293..2582
                     /gene="metK"
                     /locus_tag="PKY68_01430"
     CDS             1293..2582
                     /gene="metK"
                     /locus_tag="PKY68_01430"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOH23733.1"
                     /translation="MGYLFTSESVSEGHPDKVADQISDALVDAFLAYDENSKTACETL
                     VTTGQVILAGEVKSSIYVDVQEVARQVIKKIGYTKSEYRFDGDSCGVLSSIHEQSEDI
                     NRGVDGAAKIDPMNQGAGDQGMMFGYACKDTDNYMPLPLELSHRLLKKLAAIRREGKV
                     MTYLRPDSKSQVTIEYNDEGKPERIHTIVVSTQHDEFISAGKGLSREQADAEMLSIIR
                     KDIQQYLLPEVFNADPCLKALLDDKLILHVNPTGKFVIGGPHGDSGLTGRKIIVDTYG
                     GKGAHGGGAFSGKDPSKVDRSAAYAARHIAKNLVAAGVADEILIQVSYAIGVAHPVNI
                     FVDTYGKSRVKISDGEIAEKVVQLFDMRPKAIEQRLKLRKPIYSETASYGHFGREPQK
                     LVKHFSSRYEGDKKIEVELFTWEKLDYVEQIKKAFGL"
     gene            complement(2577..3191)
                     /locus_tag="PKY68_01435"
     CDS             complement(2577..3191)
                     /locus_tag="PKY68_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOH23734.1"
                     /translation="MRYLLFTLSLLLSINSRAQIDEGINRLLPARPPQSIPARIEGGD
                     TVLLVNLNPVYVYPGMVFKNKREERYYSRLVRDVKLCLPLARIVSGTLIETAEYLETL
                     PDDKAREKHLRQMQRDLIKDYEPTLRKMSYSQGKILLKLIVRECDSSPYEIIAAYRGY
                     FAANFWQGVAKLFTADLKTDYNPTDNQTDRLIERIIIMVEQGML"
     gene            3343..4230
                     /locus_tag="PKY68_01440"
     CDS             3343..4230
                     /locus_tag="PKY68_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23735.1"
                     /translation="MNVLRFFKRRSAAPDPSVVRGKNNILWYCALIITVLHSSPGLYA
                     QTISAREWYGDAKSMAMGDAMLISQWLFSGPSHQRQQDNMLGVQRAQAAYYLPFNRAS
                     LAVTGLMIQLRSPLADLQLNMGRSGGDDYNESLISLGCARSPTENLSGGFRLGIYSLA
                     GNQLHEGTALLTECFVYYLVNTQWRLGAYMFNPIAAKVSGIPLIQSYHLACAYRPDSR
                     LDFVLEMEKPQRQDILVHLGMEAAVLKCLRCRLGLIYPVMQPSFGLACSFKKFSLEAA
                     CRLHKQLPASYAVTLHYTP"
     gene            4301..6283
                     /locus_tag="PKY68_01445"
     CDS             4301..6283
                     /locus_tag="PKY68_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23736.1"
                     /translation="MEYILSDWVEQNPEADAEALAQELYELLEQPVNLNDAGREQLES
                     LPFLSPDQKEKLAYFLYRRKPLYSLSELLLCEGMDEVSLRRIRPFLYIGAADQTSEKV
                     DFKKLLSCGKHEIRAYTAAGFQPRQGLLNAGDSASKAEGKAYLGLPFSGVCKYRYSYG
                     RQLQYGLVMEHDLGERPVDFLSFHIAVHQLAKINTLILGDYRLKLGRGLLVSSGFSSG
                     KTTSPSALASVPKMLSSHFSCSESAYFRGAAIDLSLSEHLQLLVFASSRKLDAVLEEG
                     SFTSVRSDGLHRTSKELSYRNALRQYSTGAHIGINQSYFQAGANVLYYRFNAFCNPDL
                     KPYSLFYFRGRDNLNFSLDYRFRAGKALFSGECAFDNKFHTAHFHQLQYQPHPLLNMV
                     HSFRYYSPAYQAYFAAAFSENSRVCNETGWFTAMECRLFERLKMDTYLDVFVFPWLKY
                     GVNAPSSGHDLGLELSSCIVNGLEWGLRYREKTKTANLPVEGEKGITPCADNTRRQLR
                     LQLKACFGGLSLKTKAELNRFNIAGFEASTGALFSQECRYKTPDERLSLSLQYAMFDT
                     EKYENRIYFYEYDLPGVFSIPAYSGTGSAYVLCCKLKPGRNFQCWARLRHRAYSDREE
                     VGTMLDMVRGPCSTEFRLGFTYRFSAKSGLNSQNQP"
     gene            6408..9710
                     /locus_tag="PKY68_01450"
     CDS             6408..9710
                     /locus_tag="PKY68_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOH23737.1"
                     /translation="MKNFKLLNNVFGWFAFLVAAVTYLLTIEPTTSFWDCGEFISAAY
                     KLDVGHPPGAPFFMLTGRFFANFASGPEQVAKMINAMSALFSAFTILFLFWTITHLAR
                     KVVVKDKDNMKPAEMITVLGAGLVGALAYTFSDTFWFSAVEGEVYAYSSFFTAVVFWS
                     ILKWEEEADKPHSLRWIIFISYLMGLSIGVHLLNLLCIPALVLVVYFRKNQNANAKGA
                     ALALLGSFVLIAIVLYGVVPGFVNMAGWADLLFVNVLGFSFNSGAVAYILVMIAVLCW
                     GLAESYRGKNEFRMKLAFALNIVLSGIPFFGNSPWVWILLCGLTLAVVFRWKKINGKL
                     INTSLLALMVMLIGYFSYGVIVIRSNAQPPMDQNSPNNMFTLERYLNREQYGETPLIY
                     GQTFASDIKWKRQGNICVPQYKKRGPVWSKQVKTSPDEKDRYFISGYNERPVMADELN
                     MFFPRMHSNKPEHVVMYKDWVNFKGTPVKYNKCGQIETVYKPTMAENLRFFFSYQINF
                     MYWRYFMWNFSGRQNDIQNLGGEANNGNWITGFNFIDKHLVGDQSNLPPELANNKGRN
                     RYYMLPLLLGILGLLYQAYAGKKGVQGFWITLLLFLMTGLAIVLYLNQPPLQPRERDY
                     AYAGSFYAFCIWIGMGVPALVTILRKVLKPVPSALLAGVLGLGIPVLMASENWDDHDR
                     SDRYTARDFAYNYLIGCDENSIIYTNGDNDTFPLWYLQEVEGIRTDVRVCNLSYLQTD
                     WYYDQMLRPYYESDAFPVSWDKSKYSNGKRDIARVIPRTQDTISLPFAMNWLGSDDAS
                     TKRVQGYGDNVDHIPANKLYLDVDAEKVLENGYLEPKYAEHIVPRMFIDLSAKNYLGK
                     HEIMTLKLLEENNWERSMYYAVTVDPALMTMLQRYFLHEGLIYKLAPLESNRAINTDK
                     MYDNMVNKYRWGGIKENPDIYLDENNLRMCRTHRQHFGFLIKALYQEGKYDMALQAID
                     KCLEELPTEHVPLNFIDGGMAGMLEITEVLYDLGEKERSLAIANEGMDICIQNLNWFF
                     SLNDPLLRASGRSVNNQLYVMQELRNFLQRAATEASALENPSGVDVAFMEAFNKNTQH
                     FGQFYQQYQRLR"
     gene            9717..10322
                     /locus_tag="PKY68_01455"
     CDS             9717..10322
                     /locus_tag="PKY68_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOH23738.1"
                     /translation="MLFTQLSRCLHWIPGITWRKSASKKTIYLSFDDGPIPGVTPWVL
                     DTLDRYGIKATFFCVGENVARYPELFEQIKTRGHQTGNHSYNHLRAFRTNPDDYLANV
                     EKADKLIGSSLFRPPHGELTYAQFRALRKKYELVQWDLISRDYNAKLSPDEVFDIVRK
                     GVRKGSILVFHDSLKAEKNLRAALPRSIEFCLAQGYVFDVL"
     gene            complement(10301..11812)
                     /locus_tag="PKY68_01460"
     CDS             complement(10301..11812)
                     /locus_tag="PKY68_01460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH23739.1"
                     /translation="MEKPLISVIVPVYNMEAYLAECIDSILKSGYSPFELIIMDDGST
                     DKSLQIALEYAAKYPNIKAESQANAGVSAARNAAIELAQGKYILPVDADDLIHKDFLE
                     KAVEVLQKQPSVKVVTARAEFFGDKTGKWLLPDYSPALLARKNMLSACAMYRKSDWTT
                     CGGYCNYIPGREDWDFWISMLKNGGEVVKLNFTGFYYRYRSNSKRISDRGKKKQIIDI
                     LNQRHADFFKRELKGKLHYNRTFSKFFNSTNNLLERTKLILHEDYKNLHPFLSSLPEQ
                     FDTSGDLMFQARNTIKSYRIEGMDIVVKSYGNPLFINRIIYGNIRSSKAKRAYNYALK
                     LRNYGIGTPQPLAYIEIKKGLILHKSFFISLKSTFRNRLCDLCTETPAKDVDKLARGI
                     AVFTAKLHDLGILHKDYSSGNLLYEINKDEVLLQVIDLNRMRFGRVGRKKGCKNFERL
                     CLNDEILSRIAEEYAEVRSFDKTICVEEVLKYNRKHRNRRSKKQGQRQSTSKT"
     gene            complement(11839..12558)
                     /locus_tag="PKY68_01465"
     CDS             complement(11839..12558)
                     /locus_tag="PKY68_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH23740.1"
                     /translation="MPKPLHIITPVKDSYETAAGSIRAIKESDLPPSCSYTIYNDFST
                     ADNKKLLEELTLELGFDLVHLEDLTTNPSPNYRLVLQLAQKKALEAGADLMIIESDVY
                     IKQDTIKKMQAACAELDNPGMVAAVTVDESGVVNFPYLYASSYPSSIIKTRKRLSFCC
                     SILSNSLLSAYDFKFLDPDKSWFDVFISHKSRKLGFNNYLITKLPVLHRPHSSRPWKQ
                     LKYSRPWKYYFLKILKQRDKI"
     gene            complement(12560..>12878)
                     /locus_tag="PKY68_01470"
     CDS             complement(12560..>12878)
                     /locus_tag="PKY68_01470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOH23741.1"
                     /translation="FLDIHPLMPGHTLVIPKKETDYLFDIDDRELAAMIVFAKKIALA
                     LKTAIPCKRIGMAVVGMEVPHAHIHLIPINKETDMLFSKPKLNPTAEEMKAIAEKISN
                     SLR"
BASE COUNT         3222 a         2833 c         3154 g         3669 t
ORIGIN      
        1 tcatcccatt tgatttcgcg tatcagaagg atagagcggc cgttgcgttc catgccgtaa
       61 ccgtgaaaga acaggtaacc tttgccgtca aagccggctg cggcattgtg ccctacaccg
      121 gggaagcgtt cgttgccttt gactactaag gtccctgcgc ctttggtcat cacattgcct
      181 tccctgtcca cataagggcc ggtcactttg cgggaacggc ctaccaccac attgtaggtg
      241 ctttgggctc cgcggcagca catatcaaaa gaaacaaaaa gataatagaa actgcctttg
      301 cgaaaaataa atggagcctc gacggcacct cctccgggtt ccacatcaat atcgttaagg
      361 gtgcgtggcc tgcgcgaaag ggggtaccat tcctgaggct ctgccagggc gcagaggctg
      421 ctgtcgagtt tgaccagctt tataccttcc cagaaagagc cgaagctcat ccagcccgta
      481 ccgtcttcat ctatgatgat gttgggatct atggcattcc acatatcgcg gttgggaatg
      541 gagcgcaaca ccatgccctg atcttcccat ttgaaatcgg gcgacccggg gtcgagcgtc
      601 ttgttggtgg caacgcctat gcaggaattg ttttttccga aagtggatac cgaataataa
      661 agataatact tgccttggtg cttaataata tcaggcgccc aggtatgtcc cctgtaaccc
      721 ggcactgctt caacagccca agcgggaggc tccgaaaaaa ccggtttctc gcgtttccag
      781 ttgatcatgt cggtcgaaga aaggacggag atgccaaatc cggtacaaaa caaataatag
      841 gtattgccct gtttggccac caccggatcg tggacggagg gagtggaact gccttccggg
      901 tcaaaacgat tgttctgggc ggaaagctgc agtgtaaaac tcaataaaaa gacgatgctt
      961 gctacgaata aagcgttttt catattatat gggataaaaa gattcagccc taagtcaggg
     1021 tactgtcaac gaggctcaaa aatagctatt tttggtttaa actgaaacga tgaagcaaaa
     1081 atgtagggat ttttttgcaa gacataaaaa gccgacgtac ttttgcgggg tcttatgtgg
     1141 aaggatttgg acgcttgcaa ccacttgaaa aaatgctaaa gaatcatctt cttttctgca
     1201 atctcccctg acttccaaga cttttattaa gtgtcaccag cccctgatcc tgccggttca
     1261 gtgtatttgc gggcattaaa acagatagaa cgatgggata tttatttact tcagaatcgg
     1321 tgtccgaagg acaccccgac aaagtggccg atcagatttc ggatgctttg gttgacgcct
     1381 ttctggctta cgacgaaaat tccaaaacag cttgtgaaac tttggtaacc accggccagg
     1441 ttattctggc aggcgaagtg aagtcgagca tctatgtcga cgtgcaagag gtagccaggc
     1501 aggttattaa aaagattggt tataccaaaa gcgaatacag gttcgacgga gattcctgtg
     1561 gggtgctttc ctccatacac gagcaatcgg aagatatcaa ccggggcgtt gacggtgccg
     1621 ccaaaatcga tcctatgaac cagggtgccg gcgaccaggg catgatgttt gggtatgctt
     1681 gcaaggatac cgacaattat atgcctttgc ctttggagct ttcgcatcgc ctgcttaaaa
     1741 agttggctgc tattcgtcgc gaagggaaag taatgactta cctgcgtccc gattccaaat
     1801 cgcaggttac cattgaatac aacgatgaag gaaaacccga acggattcac accatcgttg
     1861 tttccactca gcacgacgaa tttatttcgg ccggcaaggg actcagtcgg gagcaagccg
     1921 atgccgagat gctaagtatc atccgtaaag atattcagca atacctgttg cccgaggttt
     1981 ttaacgccga tccgtgcctg aaggccttgc tcgacgataa gcttatcctg catgtaaatc
     2041 ccaccggtaa gtttgtgata ggcggtcctc acggtgacag cggtttgacc ggccgaaaaa
     2101 taattgtgga tacttacggc ggtaaaggag cccatggggg aggtgctttc tcaggtaaag
     2161 atccctccaa ggtggaccgt tcggccgcct acgctgccag gcatattgcc aagaacctgg
     2221 tggctgccgg cgtggcagac gaaattctga tccaggtatc ttacgccatt ggtgtggcac
     2281 atcctgtaaa tatttttgtc gatacttacg gtaagtctcg tgttaaaatc agtgacggag
     2341 agattgcaga aaaggtggtc cagttgtttg atatgcgtcc caaagccata gaacaacgct
     2401 tgaaattgcg taaacctatc tattccgaaa cggcttctta cgggcacttt ggcagagaac
     2461 cccagaagct ggtcaaacat ttttcgtcgc gttacgaagg agacaaaaaa atagaagtgg
     2521 aattgtttac ctgggaaaag ttggattacg tggagcagat aaaaaaggct ttcggtttat
     2581 aacattccct gttcaaccat aattattatt cgctcaatga gtcggtcggt ctgattatcg
     2641 gtgggattgt agtccgtttt aaggtctgcc gtaaacagtt tggctacgcc ctgccagaaa
     2701 ttggccgcaa aataacccct ataggctgcg attatttcgt aaggcgaaga atcgcattca
     2761 cgtacgatga gctttagcag gatttttccc tggctatagc tcatcttgcg taatgtgggt
     2821 tcataatctt tgatcagatc gcgctgcatt tgtctcaggt gcttctcgcg ggctttgtcg
     2881 tcagggagtg tttccaggta ttcggccgtt tcgataaggg tgcccgagac tatccgggcc
     2941 agagggaggc acagttttac atcgcgtacc aatcgcgaat agtagcgctc ttcacgtttg
     3001 tttttgaaca ccatgcccgg ataaacatag acgggattaa gattcaccag caacacagtg
     3061 tctccgcctt ctatccgtgc aggaatcgac tgcggaggtc gcgcaggaag cagcctgttt
     3121 ataccttcgt caatttgtgc ccggctgttt atgcttaaca aaagggacag cgtaaacaaa
     3181 agatagcgca tacaattcgt ttctggtttt gcaaaagtac ctctttaaaa ccaagtagca
     3241 aataaatgga attattgaac gggaagtgtt tatttggcaa caagtaaata gtaaagaatc
     3301 aaacaacaga cgttaaattt gcatcaatta attttgatca tcatgaatgt tttgagattc
     3361 tttaagcgta gatctgcagc tccggatccg tctgttgtcc ggggaaagaa taatatcttg
     3421 tggtattgtg ccctgataat aacggtattg cattcgtccc cgggacttta tgcacaaaca
     3481 atttccgccc gcgaatggta tggagatgcc aaaagtatgg ctatgggcga tgccatgctt
     3541 atttctcaat ggcttttttc ggggccttcc catcaaagac aacaagataa catgttgggt
     3601 gttcagcgtg ctcaggccgc ctattatttg ccttttaacc gggcttcgct ggcggtcacc
     3661 ggcctgatga ttcagttgag atctcctctg gccgatttac agttgaatat ggggcgatcg
     3721 ggcggggacg attacaacga gagcttgatc agtctgggtt gtgcacgttc accgacagaa
     3781 aatcttagcg ggggcttccg tctggggata tattctttgg ccgggaatca gcttcatgag
     3841 ggaacggctc ttctgacgga atgttttgtg tattaccttg tcaatacaca gtggaggctg
     3901 ggggcttata tgttcaatcc catagcagcc aaagtttcgg gtatacccct gattcaaagt
     3961 tatcacctgg cctgtgcata tcgtcccgat tctcgtctcg atttcgtatt ggaaatggaa
     4021 aaaccccaaa gacaggacat tctggtgcat ttgggaatgg aagctgccgt tttgaaatgc
     4081 cttcgatgca ggctgggtct catttatccg gttatgcagc cttcgtttgg cctggcctgc
     4141 tcttttaaaa aattcagttt ggaggctgcc tgccgtctgc ataaacaatt gcccgcttct
     4201 tatgctgtca cgctgcatta tacgccttga ttgcctgctt ttgctaatcc ttatttcttc
     4261 aaagctactt agtgcgcagg aatcgtatga tacggatgat ttggagtata ttctctcgga
     4321 ttgggtagaa caaaatcccg aagccgatgc cgaagccctg gctcaggaat tgtatgaatt
     4381 gctggaacaa cctgtcaacc taaacgacgc cggcagagaa caattggaat ctttgccttt
     4441 tttgagtccg gatcaaaaag aaaaactggc ctatttttta taccgtcgta aacctttgta
     4501 ctccttgtcg gagttgctgt tgtgcgaagg tatggatgaa gtgagtctca ggcgcattcg
     4561 cccttttttg tacatcggcg cagctgacca aacctcggaa aaggttgatt tcaaaaagct
     4621 cctgagctgt ggaaagcacg aaatcagggc ctacacggct gccggtttcc aacctaggca
     4681 gggactgctt aatgccggcg attccgcctc gaaagccgaa gggaaagctt accttggact
     4741 gcccttttcg ggagtttgca aataccgcta cagctatggg cgtcagttgc agtacggttt
     4801 ggtaatggaa cacgatttgg gcgaaaggcc cgtggacttt ttgtcttttc atatagctgt
     4861 tcaccaactg gcaaagatca acactttaat tctgggagat tatcgcctta aattgggccg
     4921 tggtttgctg gtttcttcgg gtttcagttc gggaaaaacc acttccccgt ctgcgttggc
     4981 atccgttcca aaaatgctga gcagccattt ttcctgttcc gaaagtgctt attttagggg
     5041 cgccgccatc gatctttcct tgtcggaaca tcttcaatta ctggtctttg cctcaagtcg
     5101 taaactggat gccgtgcttg aagagggcag ttttacctct gttcgcagcg acggcttgca
     5161 ccgtacttcg aaggagctca gttacagaaa cgctttaagg caatacagta ccggagcgca
     5221 catcggcatt aatcagagct attttcaggc cggggccaac gtgctttatt acaggttcaa
     5281 tgctttctgt aatcccgacc tcaaacccta ctccctgttt tattttagag gcagagacaa
     5341 cctgaatttt tcgctcgatt accgttttcg ggccggaaag gccctgttca gcggtgaatg
     5401 cgcttttgat aataaatttc ataccgccca tttccatcag ttgcaatatc agcctcatcc
     5461 tttgctgaat atggttcact cttttcgtta ttacagcccg gcttaccagg cttattttgc
     5521 ggcagctttt tcggaaaaca gccgggtgtg caatgaaacc ggctggttta ccgctatgga
     5581 atgccgtttg tttgagcgtc tgaaaatgga cacttacctg gatgtatttg tctttccctg
     5641 gttgaaatac ggagtcaatg ccccttcgtc gggtcacgac cttgggctgg aactcagctc
     5701 ttgcatcgta aatggtcttg aatggggcct gcgttaccgg gaaaaaacta aaacagcaaa
     5761 cctgccggtc gaaggggaaa aaggcataac accatgtgcc gacaatacta gaaggcagtt
     5821 gcgtctgcaa ttaaaagctt gttttggagg attaagcctg aaaaccaaag cggaactcaa
     5881 tcggtttaat attgccggct ttgaggcatc cacgggcgca ttgttttcgc aggaatgtcg
     5941 ttataaaact cccgatgagc ggctcagttt atctttgcaa tacgctatgt ttgataccga
     6001 aaaatatgaa aacagaatct atttttacga gtacgatctg ccgggtgttt tctccattcc
     6061 tgcctacagt ggtacgggca gtgcttatgt gttgtgctgc aagctgaagc ccggccgcaa
     6121 ttttcaatgt tgggcgagac tcaggcaccg ggcttattcc gaccgcgagg aagtggggac
     6181 tatgttggat atggtgcggg gcccttgttc aaccgagttt cgtttgggtt ttacgtatcg
     6241 tttttctgcc aaaagcggct taaacagtca gaatcaacca tagtacaggc ttgtgtttgg
     6301 atttgttttt ctatctttgc cgcttattga tgcgataatt ttatctttgt cctaaattca
     6361 acattatcat tcggggttga ttgctagccc ttttgcattg taacaacatg aaaaatttca
     6421 agcttttaaa caacgtattc ggttggttcg cttttttggt ggcagccgta acgtatttgc
     6481 tcaccattga acctacgacc agtttctggg attgcggtga attcatttcg gcggcataca
     6541 agctcgatgt gggacatcct cccggagctc cgttttttat gttgacaggc cgcttttttg
     6601 cgaatttcgc cagtggcccc gagcaggtag ctaagatgat aaatgccatg tcggcattgt
     6661 ttagcgcatt tactattttg tttttgtttt ggactattac ccatttggcc agaaaagtag
     6721 tcgttaagga taaggataat atgaagccgg cagaaatgat tactgttttg ggtgccggtc
     6781 tggtgggtgc tttggcctac actttttccg atactttttg gttctcggcc gtggagggcg
     6841 aagtgtacgc ctattcttcc ttttttacag ccgtagtctt ttggtctata ctcaaatggg
     6901 aagaagaagc cgacaaaccg cattcgctgc gctggattat ttttatctcc tatctgatgg
     6961 ggctttccat aggggtgcat ttgttgaatt tattgtgtat cccggctttg gtgctggtcg
     7021 tttatttcag aaaaaaccaa aatgccaatg ccaaaggagc cgctcttgct ttgttaggtt
     7081 cttttgtcct gatcgccata gtactttacg gtgtggttcc cggctttgtg aatatggccg
     7141 gctgggccga tcttttgttt gttaatgtgc tgggttttag ctttaactcg ggagctgttg
     7201 cctacatatt agtcatgatc gctgtccttt gttggggact ggcagaaagc taccggggca
     7261 agaatgaatt caggatgaaa ctggctttcg ctttgaatat cgtattgtcc ggtattccgt
     7321 ttttcggcaa cagcccctgg gtatggattt tgctctgcgg attgactctc gcagtggttt
     7381 tcaggtggaa gaaaattaac ggtaagctca tcaatacctc tttactggcg ctgatggtaa
     7441 tgcttatcgg ttatttcagc tacggggtca ttgtgattcg ttcaaatgca caacctccga
     7501 tggatcaaaa ttctcccaac aatatgttta ccctcgagcg ctatcttaac cgtgagcaat
     7561 atggcgaaac tcctctgatt tacggacaaa cttttgcctc ggacataaaa tggaaaagac
     7621 aaggcaatat ttgtgtccct caatacaaaa aaagaggccc ggtgtggagc aagcaggtta
     7681 aaacttcgcc cgacgaaaaa gatcgttact tcatttccgg ctataacgaa cgcccggtga
     7741 tggctgatga actgaatatg ttctttccgc gtatgcacag caataaaccg gaacatgtgg
     7801 tgatgtacaa ggattgggtt aatttcaaag gaactcccgt caagtacaat aaatgcggtc
     7861 agattgaaac cgtttacaag cctaccatgg ccgaaaacct gcgcttcttc ttttcgtatc
     7921 agattaattt tatgtactgg cgctacttta tgtggaattt ctcgggccgg caaaacgata
     7981 ttcaaaacct ggggggcgag gccaacaacg ggaactggat taccggtttt aatttcatcg
     8041 ataaacacct ggtgggtgat cagagcaatc ttccgccgga actggctaat aacaagggtc
     8101 gcaaccgtta ttatatgttg cccttgcttt tgggaattct ggggcttttg tatcaggcct
     8161 atgccgggaa aaagggcgtg cagggattct ggattacctt gcttctcttt ttgatgaccg
     8221 ggctggccat agtgttgtat ttgaatcagc ctcccttgca gcctcgcgag cgggattatg
     8281 cctacgccgg ttcgttctat gccttttgta tatggatagg catgggtgtg ccggctctgg
     8341 ttaccattct gcgcaaagtc ctgaagccgg taccgtcggc attgctggcc ggagtgctcg
     8401 ggctgggtat tcctgtgctt atggcttccg aaaattggga cgatcacgat cgttccgatc
     8461 gttatacggc ccgtgacttt gcctataatt acctgatagg ttgcgatgaa aacagcatca
     8521 tttacactaa cggcgataac gataccttcc ccttgtggta tttgcaggag gtggaaggca
     8581 taagaaccga cgttcgtgtc tgcaacctga gttatttgca aaccgactgg tactacgatc
     8641 aaatgttgag gccctattac gagtcggatg ctttcccggt gagctgggat aaatcgaaat
     8701 acagcaacgg taagcgcgat attgcccgtg ttattccccg tacccaggat acgatcagtc
     8761 tgcccttcgc catgaactgg ctgggcagtg atgacgcaag caccaagcgc gtgcagggat
     8821 acggcgataa tgtagatcac attcctgcca ataagctcta tttggatgtt gatgcagaaa
     8881 aagtgcttga aaacggttac ctggagccta aatacgctga acatatcgtg ccgcgtatgt
     8941 tcatcgattt gtcggctaag aattatctgg gtaaacacga gatcatgacg ctcaagttgc
     9001 tcgaggaaaa caattgggaa agatccatgt actatgccgt aaccgtagac cctgcgctaa
     9061 tgaccatgtt gcaacggtat tttctgcatg aagggctcat ttataagctt gcaccattgg
     9121 aaagtaacag ggcaataaat accgataaaa tgtacgacaa catggtaaat aaataccgtt
     9181 ggggaggtat caaggagaac cccgatattt acctcgacga aaacaacctg agaatgtgtc
     9241 gcactcacag gcaacacttt ggttttctga tcaaagctct ttatcaggaa ggtaagtacg
     9301 atatggcttt gcaggctata gataaatgct tggaagaact tcccactgag cacgttcctc
     9361 tcaactttat cgacggaggc atggcaggaa tgctcgaaat caccgaagtt ttatatgacc
     9421 tgggagaaaa ggaacggtct ttggccattg caaacgaagg aatggatatt tgtattcaaa
     9481 atctgaattg gttcttcagt ttgaatgatc ctcttttgcg tgcttccggc agatcggtca
     9541 ataatcaact ttacgtgatg caggaactta ggaattttct ccagcgtgcc gctaccgaag
     9601 cctccgctct ggaaaatcct tccggagtcg atgtcgcctt tatggaggcc tttaataaaa
     9661 acacgcaaca ctttggccag ttttatcagc aataccagcg gctcagataa ttagctatgc
     9721 tgtttacgca attaagtcgt tgcttgcatt ggataccggg aattacctgg cgaaagtctg
     9781 catcgaaaaa aacgatttac cttagttttg acgacggccc gatacccggt gtaacacctt
     9841 gggtattgga cacgctcgat cgttacggca tcaaagctac ttttttttgc gtaggcgaga
     9901 acgttgcccg ttatcctgaa ttatttgagc aaatcaaaac cagagggcat caaacaggca
     9961 accattctta caaccacctg agggctttca gaacaaatcc ggacgactat ctggccaatg
    10021 ttgaaaaggc cgacaaactg atagggagta gtttgtttcg tccccctcac ggcgaattga
    10081 cttacgctca attcagagct ctccggaaaa aatacgagtt ggtgcaatgg gaccttattt
    10141 cccgggatta caatgccaaa ttaagccccg atgaggtttt tgacattgtc cgtaagggtg
    10201 tcagaaaagg ctctatactt gtttttcacg attcgctgaa agcggagaaa aatctcaggg
    10261 ctgccttgcc caggtccata gaattttgtc tggcgcaagg ttatgttttt gacgtgcttt
    10321 gacgttgacc ttgctttttt gagcggcggt ttcgatgttt ccgattgtat tttaatacct
    10381 cttcgacaca gatggttttg tcgaaggagc gcacttcggc gtattcttcc gcaatccggc
    10441 taaggatttc gtcgttcaga caaagccgtt caaaattttt acagcctttt ttacggccta
    10501 ctcttccaaa gcgcatgcgg ttcagatcta ttacctgcaa aagcacttcg tctttattga
    10561 tttcgtacaa gagatttccc gaagagtaat ctttatgtaa aatacccaga tcgtggagtt
    10621 tagctgtaaa aacggcgata cctcttgcca gtttatcgac atcttttgcc ggagtttcgg
    10681 tacaaaggtc gcaaaggcga ttgcggaaag tcgatttcag actgatgaaa aaactcttat
    10741 gtaaaatcag gcctttttta atctcaatat aggccagggg ctgaggagtg ccgatgccgt
    10801 aattcctgag tttcagggca tagttgtagg ctcttttggc tttggaggat cgaatgtttc
    10861 cgtaaataat tctgttgata aacaggggat taccataaga cttcaccaca atgtccatac
    10921 cctcaatgcg ataacttttt atggtgtttc tggcttgaaa cattaaatct cccgacgtat
    10981 cgaattgttc cggcagagag gacaggaagg gatgtaggtt tttgtaatcc tcgtggagaa
    11041 ttaattttgt gcgttccagt aagttgtttg ttgaattaaa aaatttggaa aaggttctgt
    11101 tataatgcag ttttcccttt agttctcttt tgaaaaaatc cgcgtggcgc tgattcagaa
    11161 tatcgatgat ttgtttcttt ttccccctgt cgctaattct ttttgagttg cttctgtagc
    11221 gataataaaa accggtaaag tttagtttta ccacctctcc gccgtttttg agcatggata
    11281 tccaaaaatc ccagtcttcg cgtccgggaa tataattgca ataaccaccg caagtagtcc
    11341 agtcgctttt gcgatacata gcgcaggcac tcagcatgtt ttttctggcc aacaatgctg
    11401 gagaataatc cggcagcagc cattttccgg ttttatcacc gaaaaattca gccctagcgg
    11461 taacgacctt gaccgaaggc tgcttttgca acacttcgac ggctttttct aagaaatcct
    11521 tgtgaataag atcgtctgcg tcgacaggca gtatgtattt cccttgcgcc agttcgatgg
    11581 ctgcgtttct ggctgcagac accccggcat tggcttgaga ttctgccttg atgttggggt
    11641 attttgctgc gtattcgaga gctatctgca atgacttgtc ggtagagccg tcgtccataa
    11701 taatgagctc aaaaggggaa tatcccgatt tcaggatgga atcgatgcat tccgccagat
    11761 aggcttccat attataaaca gggactatta cagagatcaa aggcttttcc atagtcgtgt
    11821 tgtaagataa gcaactcgtt aaattttgtc gcgctgctta agaattttca ggaagtaata
    11881 cttccagggc cgggaatatt tcaattgttt ccagggcctg ctgctgtgtg gtcggtgcag
    11941 taccggcaat ttggtaatca ggtaattgtt gaaaccgagc ttgcgtgatt tatgtgagat
    12001 aaatacatcg aaccaggatt tgtccggatc cagaaattta aaatcatagg ccgataaaag
    12061 actgttgctt agaatagagc agcaaaaact cagccgtttg cgggttttga tgatggatga
    12121 ggggtaggag gaggcataaa gataagggaa attgacaact ccactctcgt caaccgtaac
    12181 ggcggccacc attccgggat tgtcgagttc agcacaggca gcctgcattt tctttatggt
    12241 gtcttgttta atgtagacat ccgattcaat gatcatcagg tctgctcccg cttcgagggc
    12301 ttttttttga gcaagttgca gcaccagtcg gtagttaggc gagggattgg tggtcaaatc
    12361 ttccaggtgc accaagtcga aaccgagttc cagagttaat tcttcgagga gttttttgtt
    12421 gtccgccgta ctgaagtcat tgtaaatagt ataggaacag gaaggaggta aatccgattc
    12481 ctttatggcc ctgatactcc cggcggcagt ttcataagaa tctttgaccg gggtaatgat
    12541 gtgcaaaggc ttaggcatgc tatctcaaag agttgctgat tttttcggct atagccttca
    12601 tttcctccgc tgtcggattg agtttgggct tagaaaaaag catatctgtc tccttgttta
    12661 tggggatcag gtggatgtgt gcatgaggaa cttccatacc gaccacagcc atccctatgc
    12721 gtttgcaggg tatggcggtt ttaagtgcca atgcgatctt tttggcaaag actatcatgg
    12781 ctgcgagctc ccggtcgtcg atatcgaaca gataatctgt ttcttttttg ggtattacca
    12841 gtgtatgtcc cggcatcagg ggatgtatat ccagaaag
//
LOCUS       DAOSGL010000021        12867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4074_length_12867_cov_5.034265, whole genome shotgun sequence.
ACCESSION   DAOSGL010000021 DAOSGL010000000
VERSION     DAOSGL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4074_length_12867_cov_5.034265"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1007
                     /gene="xerD"
                     /locus_tag="PKY68_01475"
     CDS             114..1007
                     /gene="xerD"
                     /locus_tag="PKY68_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOH23742.1"
                     /translation="MTTASDCIRKYRAFLKLEKSLSENTLAAYMSDLQKLMDFSGDKT
                     LTELKHEDLEEFLCHLRDLGIGARSQARILSGIKSFYGFLLIEDYISSDPTELIETPR
                     LGKKLPEVLSVDEIDKLLASIDLSRKEGQRNKAMLETLYSCGLRVSELTGLKLSCCYF
                     KEEYLLVEGKGSKQRLVPISRKAMKEISLWLIDRNLLPKQKNQEDFVFLNRRGHKLSR
                     SMVFKIIKEQALLAGINKNIGPHTLRHSFATHLLEGGANLRVIQQMLGHENIQTTEIY
                     THLDSAYLRQQLMEFHPRNTL"
     gene            1020..1889
                     /locus_tag="PKY68_01480"
     CDS             1020..1889
                     /locus_tag="PKY68_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOH23743.1"
                     /translation="MRIVVLAKQVPDTRNVGKDAMKEDGTVNRAILPAIFNPEDLNAL
                     EMALGIKEQFPGTTIQVLTMGPPRAAELIREALYRGADGGILLSDKAFAGADTLATSY
                     ALSKAIQKMGQVDLIIGGRQAIDGDTAQVGPQVAEKLNLYQVTYAEEILELDPKNKQV
                     RIKRRLPRGIEVVLSPMPVLITVNGSAKACRPRNAKRVLKYKYALTRFETEENPKFKA
                     LCDQRSYLNIPVWDVDFIEADKDLCGLSGSPTKVKKIESVVFQAKENMTLESTDAEIE
                     TLIKTLLENHTIG"
     gene            1917..2789
                     /locus_tag="PKY68_01485"
     CDS             1917..2789
                     /locus_tag="PKY68_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOH23744.1"
                     /translation="MNRNVYIRRAKFFFAGLLAAFLLASCGSVFLTGRKQLMLVSDQE
                     VLALSLQQYRSFMDSAEVVVNTADAEMINRIGRSIAESVENFMTFNGRAHELENIDWE
                     FSLVKDNSVNAFAMPGGKVVVFTGMLPVAESEAHLAVVIAHEIAHVIAKHSTERISQQ
                     IALQFGGEIAGGLLASSSETKRSIATTVFGLGAQLGVMMPYARKQEYEADELGLLFMA
                     MGGYHPQEAAAFWTKMSAAGGTAIPEFLSTHPTDAKRIAKIQQKMPDALYYYKGPGII
                     NRTTAPIVKDTKVL"
     gene            complement(2793..4289)
                     /locus_tag="PKY68_01490"
     CDS             complement(2793..4289)
                     /locus_tag="PKY68_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOH23745.1"
                     /translation="MTGIKSLAKDTAIYGLSSIVGKFLNWLLVPFYSYTLKETGDYGM
                     VTELYGWTALALVILTYGMETGFFRFINNDKYKSTQVYSTSLSMLGLTSLLFVAVSLI
                     FLPQISAGMGYEKHPEFIGMLVVVVAADAFCSIPFAYLRYKNKALRFASIKLLMILTN
                     IFFNLFFIWLCPKIYRWNPALIDWFYKPDYGVGYVFVSNLFSTGITLLALLPYFVKVK
                     FDFNPVLLRKMLGYSLPLLLLGIAGIMNQTFDKIIFTHLFEDQQYARAQLGIYGACYK
                     IAVVMMMFTQAFRYAYEPYIFAKHRDDDNRRAYAEAMKYYIIFAFLIFLGIMFYLDIF
                     KYIISSDYHEGLKVVPIVLMCYLFQGVFFNLSLWYKLTDRTQWGAYISMFGAFITVLG
                     NILFVPRYGYMAAAWVSFVCFFLMMVISWWLGQKYYPIRYDLKSAGRYLLLTAGLYAL
                     GMLLPVEPLWLKLLFRTALLLIFLLVLTKKDLPLNQLLPGKLLHKKTK"
     gene            complement(4296..5318)
                     /gene="ruvB"
                     /locus_tag="PKY68_01495"
     CDS             complement(4296..5318)
                     /gene="ruvB"
                     /locus_tag="PKY68_01495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOH23746.1"
                     /translation="MDDQFDIRSPRLNESEQDFEKVLRPLSFADFRGQEKVVENLKIF
                     VSAASMRNESMDHVLLHGPPGLGKTTLSNIIANELGVGFKSTSGPVLDKPGDLAGVLT
                     ALEPNDVLFIDEIHRLSPVVEEYLYSAMEDYRIDIMIDKGPSARSIQLDINPFTMIGA
                     TTRSGLLTAPLRARFGINCHLEYYQVEVLSGIISRSARILDVPCSTEAAFEIARRSRG
                     TPRVANALLRRIRDFAQVKGNGKIDLNITKYALLALNIDTHGLDEIDNRILTTIIDKF
                     QGGPVGLTTIATALGEDPGTLEEVYEPFLIQEGFLKRTPRGREVTELAWRHLGRNYQG
                     DFQRLF"
     gene            5630..6868
                     /locus_tag="PKY68_01500"
     CDS             5630..6868
                     /locus_tag="PKY68_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOH23747.1"
                     /translation="MVLNYIWVGFFVVAFVFALIQSLFFNDYAVFERIMNSSFDMAKF
                     AVMDICLPLVGIMSLWLGFMNIGEKAGAIRFLSRIVGPFFSKLFPGVPKDHPVNGELL
                     MNFSANMLGLDNAATPLGLKAMQSLQELNPHKESASNAQIMFLALNTSGLTIIPISII
                     AQRAILGAANPTDVFVPIIIATYVSTLTAILYVGFRQRLSLFNKTVVAWLGGISLLLA
                     ALVFWFTQMDKESMRTASSLLSNGTLLGIIILFMAGGLYKKINIWESFIDGAKNGFKT
                     SVKIIPYLVGMLVGIGVLRASGALDFLTDGIAWLFNGLRINTDFVDALPTALMKPLSG
                     SGARAMMIEAMKMEGADSFVGRLACVFQGSTDTTFYIIALYFGSVGIKRTRYAVSAGL
                     IADLAGIIASILLAYLFFHR"
     gene            6877..7314
                     /gene="ybeY"
                     /locus_tag="PKY68_01505"
     CDS             6877..7314
                     /gene="ybeY"
                     /locus_tag="PKY68_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOH23748.1"
                     /translation="MIQFHREDVAMPELDKNMICAWVEEVAAGYGKKTGQINYIFCSE
                     NKIAEINMRYLNHDYFTDIIGFDYSSGESISGDLFISPLTIASNAELLKQPFQRELHR
                     VLIHGILHLCGFDDKSTNQQAIMREEENKALELLYIIDKEQGS"
     gene            7329..9203
                     /gene="mnmG"
                     /locus_tag="PKY68_01510"
     CDS             7329..9203
                     /gene="mnmG"
                     /locus_tag="PKY68_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOH23749.1"
                     /translation="MNFSYDIIVVGAGHAGCEAATAAANMGSKTLLITMDMNKIAQMS
                     CNPAMGGIAKGQIIREIDALGGYTGIVSDKTAIQFRMLNRSKGPAMWSPRSQNDRQQF
                     IQAWRQVVENTDNLFLWQDTVNELLIENGTVKGVKTELNVIFRAKSVILTNGTFLNGL
                     IHIGQNQIKGGRISERASVGLTEQLRELGFSYGRMKTGTPVRVDARTVDFSKLSEQKG
                     ENDFHKFSYLDFTPRKLKQRSCWICFTNERVHELLREDLELSPMYNGQIQSIGPRYCP
                     SIETKIVNFSDRDQHQMFLEPEGEDTQEYYLNGFSSSLPLETQLKALREVSGFEQVHI
                     YRPGYAIEYDYFDPVQLKATLETKLIPGLFFAGQINGTTGYEEAAGQGLVAGVNAHIF
                     CHGGQEFVLNRDEAYIGVLIDDLINKGVDEPYRMFTSRAEYRILLRQDDADARLTEKS
                     YALGLATTERLNLWKEKQKEIESLLQFARQYSIKAKYINTALESLDTAPLKQGVRLSD
                     LILRPQLSFDILQEHIPALKRRIEQIPNRRDEIVEAAEIQIKYQGYIEREQLQAEKTQ
                     RLDNIRIKDKFDYLSIHSLSFEARQKLDKIQPETLGQASRIPGISPSDISILLVLLGR
                     "
     gene            9237..11084
                     /gene="uvrC"
                     /locus_tag="PKY68_01515"
     CDS             9237..11084
                     /gene="uvrC"
                     /locus_tag="PKY68_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOH23750.1"
                     /translation="MQTPSSHINQILSGLPELPGIYQYLGEEGRIIYVGKAKNLKKRV
                     LSYFNKNHNDSPKTAILVKRIRDIRYTVVENEQDALLLENNLIKEHQPRYNVLLKDDK
                     TYPSICIKNECFPRVFQTRRILKDGSEYFGPYSSVPMVKAILDLIGKLHPLRSCSLDL
                     SEKKISSGKYRVCLDYHIKKCQGPCVGLQSEADYNKNIDEIRHILKGNLETVEEILKK
                     EIKEHAEALRFEEAQNCKNKMELLDSYRSKSYIVNSNIRELDVFSWDETENAAFINYL
                     HVHNGCIVQTYTFEYRKKIEEDKESLLASGIYEMRSRFKSESKEVVVPFIPSMELDNV
                     KFHVPKRGDRKKLLDLSKNNVRQYKMDLLKQEEKLNPEQRSTRILQTLKKDLQLSELP
                     VHIECFDNSNIQGSSPVASCVVFKKGKASKKDYRHFNIKTVPGIDDFASMKEIITRRY
                     MRQAREEQGIPQLVIVDGGKGQLSSAVEAHRHIIELCKKENIDEKILSKIQSIRLIGI
                     AKRLEEIYFPNDPTPLYLNKNSESLKLIQQIRDEAHRFGITFHRKLRSKVQIKSELDE
                     IPGIGKVTKNLLLEHFKSLKKIREADSNELEKIVGKSKANNIINYFKSK"
     gene            11081..11530
                     /gene="dtd"
                     /locus_tag="PKY68_01520"
     CDS             11081..11530
                     /gene="dtd"
                     /locus_tag="PKY68_01520"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOH23751.1"
                     /translation="MKTVVQRVRRASVSVNKQLCGSIDKGLLVLIGIEDNDGPDDLIW
                     MAEKICHLRIFNDEKGVMNLSIMQDGGRLLVVSQFTLCANTEKGHRPSYIKAAKPEKA
                     KAMYEDFCSFCEKILNKKTEQGVFGAHMLVELINDGPVTILLDSKNR"
     gene            11533..11862
                     /locus_tag="PKY68_01525"
     CDS             11533..11862
                     /locus_tag="PKY68_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOH23752.1"
                     /translation="MTLKDAQIQVDAWIKQYGVRYFNELTNMALLCEEVGEVARIIAR
                     EYGEQSKKPNENTAKLSDELADVLWVLICLANQTGIDLEQAFAENIKKKNLRDKSRHQ
                     ENKKLYA"
     gene            complement(11917..>12867)
                     /locus_tag="PKY68_01530"
     CDS             complement(11917..>12867)
                     /locus_tag="PKY68_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOH23753.1"
                     /translation="KEFLLYQETYKKALEPENPILSEVVQYLNGSLGKEIRPTVLLLS
                     SAMFGPVEQTSINIAASLQILHTASLLHDDVIDETYQRRSKNSINAIWKNKVAILSGD
                     YYLTKVIEIAALTGRLDIITEIGKLGASLADGELVQLHVSQQQNPTEAIYFDIINKKT
                     AQLFAFCAKAGAMASSAKPEAINRLALFGEYLGIIFQLKDDSFDYILPGQSGEIPGKP
                     VANDLKEGKVTLPLIYALGHSDEKKSRPYREIMAKRHFTKYNVRRMYRFAIESGGLDY
                     LSKKMEEYKQKAEKILAEFPPSDAKDSLNLCLEYAANRCI"
BASE COUNT         3912 a         2821 c         2861 g         3273 t
ORIGIN      
        1 atggcaattt tcatacgttt aaaatttagc tgttcctcta caatttattt actacttttg
       61 ccccgcctgc cgcgtgtgcg gtttattttc tgcctgacaa aggtaaaaaa aatatgacga
      121 cagcttccga ttgcataaga aaataccggg cttttctcaa actggagaaa tctctgtcag
      181 aaaacaccct tgctgcctat atgagcgatt tgcaaaagct tatggacttt agcggcgaca
      241 aaaccctgac agaacttaag cacgaggact tggaagaatt tctgtgtcat ctgagagatc
      301 ttggtatcgg tgcgcgctca caagccagaa tactttccgg aatcaaatct ttttacggct
      361 tcttgttgat cgaagattat atttcgagcg atcccaccga actcatcgag acgccccggc
      421 tcggtaaaaa actgcccgag gtgctctccg tagacgaaat cgacaaactt ttggccagta
      481 ttgacttgtc gcgtaaagaa ggccaacgca acaaagccat gctggaaaca ctctacagct
      541 gcggcctccg ggtttcggag ctaaccggac tgaaactctc gtgctgttac tttaaagagg
      601 aatatttact ggtcgaaggc aaagggagca aacagcgatt agtgcccatt tcccgcaaag
      661 cgatgaagga aatctcgctt tggttaatcg acagaaatct gctgcctaaa caaaaaaatc
      721 aggaagattt tgtatttttg aaccgtcgcg gacataaact ttctcgttcc atggttttta
      781 aaataatcaa agagcaagcc ctgttggccg gtatcaataa gaatatcggg cctcatactt
      841 tgaggcattc ctttgccact cacctgcttg agggaggggc caatctgcgc gtaatccaac
      901 aaatgctcgg acacgagaac attcagacca ccgagatata tacccatctc gactctgcct
      961 acttaagaca acaactgatg gaattccatc caagaaacac tctataaaat cctttaatta
     1021 tgagaatcgt cgttttagct aaacaagtac ccgatacccg caatgtggga aaagacgcca
     1081 tgaaagagga cggtaccgtc aacagagcca ttttaccggc cattttcaac cccgaagacc
     1141 tcaacgcact cgaaatggca ttgggcatca aagaacaatt cccgggcact accattcaag
     1201 tactaaccat gggccctccc cgggcggccg aattgattcg ggaagccctg tacagagggg
     1261 cagacggggg catcttgttg agcgacaaag cctttgcagg agccgacacc ctggctacct
     1321 cttacgccct ctcgaaagcc atccaaaaaa tgggacaagt cgatttaatc attggaggaa
     1381 gacaagccat cgacggagat accgcacaag tgggccccca ggtagccgaa aaactcaact
     1441 tataccaggt aacctacgct gaagaaattt tggaactcga tccgaagaac aaacaagtgc
     1501 gaattaaacg tcgtctgccc agaggaatcg aagtggtgct ttcgcccatg cccgtgctga
     1561 ttacggtaaa cggctctgcc aaagcctgca ggccgcgtaa cgccaaaaga gttcttaaat
     1621 acaagtacgc tcttacaaga ttcgaaaccg aagaaaatcc taaatttaaa gccctttgcg
     1681 accaaagaag ctatttaaac atcccggttt gggacgtgga ttttattgag gccgataaag
     1741 atctttgcgg tttatccggc tctccaacca aggtcaaaaa aatcgaaagc gttgttttcc
     1801 aggccaaaga aaacatgacg ctcgaatcga cagatgccga aatcgagacc ttgatcaaga
     1861 ctttgcttga aaaccacact ataggataag tattactact ttcattattg aatcctatga
     1921 acagaaatgt ttacatccgg agggcaaaat ttttcttcgc cggcttattg gctgcttttc
     1981 ttttagcatc ctgcggcagc gtttttctta ccggaagaaa acaactgatg ttggtttccg
     2041 accaggaagt gctcgcatta agcttgcaac aatatcgcag ttttatggac agcgcagaag
     2101 tcgtcgttaa cacggccgac gcagaaatga tcaatcgtat cggtcgttcg atagcggaat
     2161 ctgtagaaaa ttttatgaca ttcaacggta gagcccacga actggaaaac atagactggg
     2221 aattcagcct ggttaaagac aacagcgtca acgcttttgc catgcccggc ggcaaggtgg
     2281 ttgtttttac cggtatgctt cccgtggccg aaagcgaagc gcacttagct gttgtcattg
     2341 cacacgaaat cgcccatgtc atcgccaaac attccacgga aagaatcagt caacaaatcg
     2401 ccctgcaatt cggcggggaa attgccggcg ggcttttggc cagctcgtct gaaaccaagc
     2461 gaagcattgc caccacagtt ttcggactgg gagcccagct gggagtaatg atgccttacg
     2521 cccgcaaaca ggaatacgag gccgacgaac tgggtctgct tttcatggca atgggcggct
     2581 accatcctca ggaggctgct gctttttgga ccaaaatgtc ggcggccggt gggactgcaa
     2641 ttccggagtt tttgagcact cacccaaccg atgccaagcg catagccaaa attcagcaaa
     2701 agatgcctga tgctctgtac tattacaaag ggccgggcat cattaacaga accacggccc
     2761 ctatagtcaa agacactaag gttttgtaaa tcctacttag tctttttatg cagaagttta
     2821 cccggaagca attggttcag gggtaggtct tttttagtca ataccagaag gaaaatcagc
     2881 agaagcgccg ttctgaaaag taatttcagc cacaaaggct caacaggcag aagcatcccc
     2941 agagcataca ggccggcggt aagcagtaaa tatcttccgg ccgatttcag atcatagcgg
     3001 atggggtaat acttttgccc caaccaccag gaaatcacca tcataagaaa gaaacagacg
     3061 aaagaaaccc aagctgccgc catatagccg tagcggggta caaaaagaat gtttcccaaa
     3121 acggtaatga aagcgccaaa catggaaata taagcacccc attgggttcg gtcggtcagc
     3181 ttataccaga gagaaagatt gaaaaagact ccctgaaaca gatagcacat caaaacaatg
     3241 gggactacct tcaagccctc gtgataatcg gagctgataa tgtatttgaa aatgtcgagg
     3301 taaaacatta tccccaggaa aataagaaaa gcaaaaatga tgtaatactt cattgcttcg
     3361 gcatacgcgc gtcgattatc gtcgtctcgg tgtttggcaa aaatataggg ttcgtaggca
     3421 taacgaaaag cctgagtaaa catcatcata accacggcta ttttataaca agctccgtaa
     3481 atacccaatt gagccctggc gtattgctgg tcttcgaaca aatgagtaaa aataatctta
     3541 tcgaaggtct ggttcatgat accggcaatt ccaagcaaaa gcaggggcag tgaatagcca
     3601 agcattttcc tcagtaaaac cggattgaag tcgaatttta ctttgacaaa atagggcagt
     3661 aaggccagca gtgttatgcc cgtagagaat aaattggata caaaaacgta acccaccccg
     3721 taatcgggct tgtaaaacca atcgatcaaa gctggattcc accggtaaat ttttggacag
     3781 agccaaataa aaaacaggtt gaaaaagata ttggtgagta tcatcaataa cttgatcgag
     3841 gcgaaacgca aagccttatt tttgtaacgt aaataagcaa agggaataga gcaaaaggca
     3901 tcggcggcca caacaacaac cagcatccct ataaattcag ggtgcttttc atagcccatg
     3961 ccggccgaaa tctgaggtaa aaaaatcaaa gaaaccgcca caaaaagcag ggaggtcaat
     4021 cccaacatgc tcagactggt ggaataaacc tgggtcgact tatacttgtc gttgtttatg
     4081 aagcggaaaa atccggtttc catgccgtag gtcagaatca ccaaggctaa agccgtccac
     4141 ccgtatagct cggtgaccat gccgtaatcg ccggtttctt tgagtgtgta ggagtaaaag
     4201 ggaaccagca gccaattcaa gaattttccg acgatactgc ttaagccata tattgcagtg
     4261 tccttggcca acgatttgat tcctgtcatt ccgtcttaaa acaaacgttg aaaatctccc
     4321 tggtaattgc ggcctaaatg cctccaagcc aattcggtaa cctctcttcc cctgggtgta
     4381 cgttttagga aaccttcttg aatcaaaaaa ggttcataca cctcttccaa tgtgccggga
     4441 tcctcgccta gagccgttgc aatagtggtc aggcccaccg gcccaccctg gaatttatcg
     4501 ataatggtgg tcaggattct gttgtcgatt tcatccaggc cgtgagtatc aatattcagg
     4561 gccaataagg catatttggt aatattcaag tcgatctttc cgttgccttt tacttgggca
     4621 aaatcgcgaa tacgtcgcag caaggcgtta gctacgcggg gcgtgcccct gcttcgccgg
     4681 gcgatttcga aggcagcttc ggtactgcag ggcacgtcga gtattcgggc cgaccttgat
     4741 attatgccgg acaaaacctc aacctggtag tactccaaat gacaattaat gccgaaacga
     4801 gccctcaggg gagcagtaag cagaccgctg cgagtggtgg ccccaatcat ggtaaatgga
     4861 ttgatgtcca actgtattga ccttgcgcta gggcctttgt ctatcataat gtcgatgcgg
     4921 taatcctcca tggccgaata caggtattct tcgaccacag ggctcaagcg atggatttcg
     4981 tcgatgaaca gaacatcgtt gggttccagg gcagtcaaca ctccggccaa atcgcccggc
     5041 ttatccaaca cagggcccga agtggatttg aaacccacac ccaattcgtt ggcaattata
     5101 ttggacaaag tcgttttgcc caatcccgga gggccatgca acaaaacgtg atccatggat
     5161 tcattgcgca tcgaagcggc agaaacaaaa atcttgaggt tctctaccac tttttcttgt
     5221 cctcgaaaat cggcaaaact gagcggacga aggacttttt cgaaatcctg ttcgctttcg
     5281 ttcagcctgg ggctgcgtat gtcaaactga tcgtccatgt tgtgatgtga ggctcaaaga
     5341 tacacgcatt cggggaattt agaaagagcg gggagaagaa tttttgggct gcgcccaaga
     5401 ataagcgttc caattgatcg atagctcccc gaagagactt acgctatgtt tttgggctac
     5461 gcccaagaat atagcttcgc tcggcaatag ccaaaatatt tttggctatt gctctcatgg
     5521 ggatattttt gggctacgcc caagaatata gcttcgctcg gcaatagcca aaataaattt
     5581 tggctattgc tctcacttac gctatatttg cccacataaa aggaagtgta tggtgctcaa
     5641 ttatatttgg gtaggttttt tcgttgttgc ttttgttttt gctctgattc aaagcttgtt
     5701 ctttaacgat tacgcggttt ttgaacgaat catgaacagc agttttgata tggcaaaatt
     5761 cgctgttatg gatatctgcc tccctttggt gggcattatg tctttgtggc tgggctttat
     5821 gaacataggc gaaaaagcgg gggctattcg ttttttatcc cgtatagtgg gacctttctt
     5881 ttcaaaactg ttccccgggg tacccaagga tcatccggtc aacggtgaat tattgatgaa
     5941 tttttcggct aatatgttag ggttggacaa tgccgccact ccactgggct taaaagcgat
     6001 gcaaagcctg caggagttga atccccacaa ggagtctgcc tccaacgctc aaattatgtt
     6061 tttggccttg aacacctcgg ggctcaccat tattcctatc agcataattg cccaacgagc
     6121 catactcggc gcagcaaacc cgacggatgt atttgttccc attattattg ccacctatgt
     6181 gtctactctg acggccatac tttacgtggg ttttcgccag cgcctgtcgc tgttcaacaa
     6241 aacggtggta gcctggctgg gaggtatcag cctgttgctt gcagccctgg tgttttggtt
     6301 cacgcaaatg gacaaagaaa gcatgagaac agcctccagc ctgctgagta acggcacttt
     6361 gctgggtatt atcattttgt tcatggccgg tggtttatac aaaaaaataa acatctggga
     6421 aagctttatc gacggtgcca aaaacggttt caaaacctct gtgaaaatca tcccttatct
     6481 ggtgggtatg ctcgtaggaa tcggagtttt aagagcatcg ggtgccttgg attttcttac
     6541 agacggcatc gcctggcttt tcaacggcct gcgcatcaat accgattttg tggatgcgct
     6601 acctacagct ttgatgaaac cattgagcgg aagtggcgcc agggccatga tgatagaggc
     6661 aatgaaaatg gaaggggccg attccttcgt aggccggctg gcttgtgtat ttcagggatc
     6721 gaccgatact accttttaca tcattgcgct gtatttcgga tcggtgggta taaaacgcac
     6781 acgctatgct gtatcggccg gattgatcgc tgatcttgca ggcatcatag cttccatctt
     6841 gctggcttat ctgttttttc atagataaac ttacttatga ttcaatttca ccgggaagac
     6901 gttgccatgc cggaactgga taaaaacatg atctgcgcct gggtagaaga agtggccgcc
     6961 ggttacggca agaaaaccgg tcaaatcaat tacatttttt gctccgaaaa taaaatagct
     7021 gaaattaata tgcgatatct taaccacgat tattttaccg atatcatcgg tttcgattat
     7081 agttcagggg aaagcatttc gggggattta tttatttctc ccttgactat cgcatccaac
     7141 gccgagcttt taaaacagcc ttttcaacgg gaactgcaca gagttttaat tcacggcatt
     7201 ttacatcttt gcggcttcga tgacaaatcg acaaatcaac aagcaattat gcgcgaggaa
     7261 gaaaacaaag cactcgaatt gctttatatc atagacaagg aacaaggatc ctgaaatgaa
     7321 ttttcaccat gaacttttcg tacgacataa tagtagttgg agcaggtcac gccggctgtg
     7381 aagccgccac tgctgccgcc aacatgggct ccaaaaccct gttgattact atggatatga
     7441 acaaaattgc ccagatgagc tgcaaccctg ccatgggagg aatagccaaa ggccagatta
     7501 tcagagaaat agatgcgctt ggaggctata caggcatcgt tagcgacaaa acagccattc
     7561 agtttcggat gctcaataga tccaaaggcc ctgccatgtg gagtccacgc tcacaaaacg
     7621 accggcagca atttattcag gcatggcgac aagttgtaga aaataccgac aatctattcc
     7681 tttggcagga tacggttaat gaattattga tagaaaatgg taccgtaaaa ggggtaaaaa
     7741 cggaactcaa cgtaattttc agagccaaaa gtgtcatact taccaacggt acttttctaa
     7801 acggattgat acacatcgga caaaatcaaa ttaaaggagg ccggatttca gaaagagcct
     7861 ccgttggttt aactgaacaa ctgagagaac tgggattcag ctacggcaga atgaaaaccg
     7921 gcactccggt gcgtgtggac gcaagaacgg tagattttag caagctaagc gaacaaaagg
     7981 gggaaaacga ttttcataaa ttttcttacc tcgacttcac accaaggaaa ctaaaacaac
     8041 gcagctgttg gatctgcttt actaacgagc gtgtacacga acttctgaga gaagacctgg
     8101 aactctcgcc catgtacaac ggacaaattc aaagcatagg gccccgctat tgccccagta
     8161 ttgaaaccaa aatcgttaat ttcagcgata gggatcaaca ccagatgttt ttggagccgg
     8221 aaggagagga tacccaggaa tactacctga atggtttttc ctcttccctg cccttggaaa
     8281 cccaactaaa agcgcttagg gaagtttccg gctttgaaca agtccatatt taccgtccgg
     8341 gctatgccat agaatacgac tactttgatc cggtccaact caaagccact ctggagacca
     8401 agttgatccc cggtttattt tttgccggcc agatcaacgg caccaccggc tacgaggaag
     8461 cagccggtca aggcttggtg gctggagtca acgcacatat tttttgccac ggaggccaag
     8521 aattcgtatt aaacagagac gaagcctata ttggcgttct tattgacgat ttaatcaaca
     8581 agggggtaga cgaaccctac cgcatgttta cttccagagc cgagtaccgc atattgttaa
     8641 gacaagacga tgccgacgct cgtctaactg aaaaatcgta tgccctgggg ctggctacaa
     8701 ccgaacgcct gaatctttgg aaagaaaaac aaaaagaaat tgagagcctg ctgcagtttg
     8761 cccggcagta ttccattaag gctaaatata taaacaccgc cttggagagt ctcgatacag
     8821 ctccccttaa gcaaggagtc cgcctgagcg atctgatttt gcgcccacaa ttgtcttttg
     8881 atattttgca agaacatatt cccgcactca aaagaagaat tgagcagatc cccaatcgcc
     8941 gggacgaaat agtcgaagcg gccgaaatcc aaatcaagta ccagggttat attgaacgcg
     9001 aacagttaca ggcagaaaaa acgcaaaggc tcgacaatat tcgaatcaaa gataaattcg
     9061 actatttaag catacattcc ctatcgttcg aagcaagaca aaaattagat aaaatacagc
     9121 ccgagacttt aggtcaggcc agccgtattc cgggaatctc tcccagcgac atcagtattt
     9181 tgctggtttt gctggggaga taagcccgat ttgttccacg tgaaacattt aagccgatgc
     9241 aaacgccttc gtcacatata aaccaaatat taagcgggct gcccgaactc cccggcattt
     9301 atcagtactt gggagaagag ggccgcatca tttatgtggg caaggcaaag aatttaaaaa
     9361 aaagagtcct ctcgtatttt aataagaatc acaacgattc tccaaaaacg gccattttag
     9421 tcaaaaggat cagagatatt cgctatactg tggtcgaaaa cgaacaagac gctcttttgc
     9481 tcgaaaacaa cctgataaaa gaacaccaac ccaggtacaa tgttttgctg aaagacgaca
     9541 aaacttaccc cagtatttgc atcaaaaacg aatgtttccc cagagttttc cagactcgaa
     9601 gaatcctaaa agacgggtca gagtattttg gtccgtacag ttctgtaccc atggtcaaag
     9661 ccattctgga tttaattggc aaactacatc cattacgaag ctgttcactg gatttatctg
     9721 agaaaaaaat ctcgtcgggg aaatatcgag tttgcctcga ttaccatatc aaaaaatgcc
     9781 agggtccctg tgttgggcta cagtccgaag ccgattacaa caaaaacatt gatgagattc
     9841 ggcatattct caaaggcaac cttgaaacag tggaggagat attaaaaaag gaaatcaaag
     9901 aacatgcaga agctttacga tttgaagagg cacagaactg taaaaacaaa atggagttgt
     9961 tggattcata ccgcagtaaa tcttatatag taaattccaa catacgcgaa ttagatgtct
    10021 ttagctggga tgaaacagag aatgctgcct ttataaatta ccttcacgtt cataatggct
    10081 gtattgtaca gacttacacc tttgaataca ggaaaaaaat agaagaggac aaagaatctc
    10141 tgcttgcaag tggtatttat gagatgcgga gtcggttcaa aagcgaaagt aaggaagttg
    10201 tggtgccctt tattccctcg atggaattgg ataatgttaa atttcatgtc cccaagcggg
    10261 gtgatcggaa aaagctgctt gatttatcaa aaaacaacgt tcgtcaatat aagatggatc
    10321 tgctcaaaca agaagaaaaa ttaaatccgg aacaacgaag cacccgaata ttacaaacgc
    10381 taaaaaaaga cctgcagctt tcagaacttc ccgttcacat cgaatgtttt gataattcaa
    10441 atattcaggg cagcagccct gtggcttctt gtgttgtttt taaaaagggg aaagcatcaa
    10501 aaaaggatta caggcatttt aatattaaaa ctgttcccgg aatagatgat tttgcatcaa
    10561 tgaaagaaat cattactcgc aggtatatgc gccaggccag agaagaacaa gggataccac
    10621 aattggtcat tgtggacggc ggaaaggggc aattgagttc agcagtggag gctcatagac
    10681 acatcattga gctttgtaaa aaagaaaaca tcgacgaaaa aattcttagt aaaatacaat
    10741 caatcagact aattggcatt gcaaaacgac ttgaagaaat ttactttcca aacgatccta
    10801 ctccgttata tttgaacaaa aattcagaaa gtttgaaatt aatacaacaa ataagggatg
    10861 aagcacatcg ttttggaata acttttcacc gtaaactcag aagcaaagtt cagataaaat
    10921 cggaactgga tgaaattcca ggcattggta aggtcacaaa aaatctgcta ctggagcatt
    10981 tcaaaagttt gaaaaaaata cgggaagccg atagcaatga attggaaaaa atagtgggaa
    11041 agtctaaagc caataacata ataaattact ttaaatcgaa atgaaaacag ttgtacaaag
    11101 agtaagaagg gcctctgttt ctgtaaataa acaactatgt ggtagtatcg acaagggctt
    11161 gcttgtatta atcggaattg aagataacga tgggccagat gacctgatct ggatggcaga
    11221 aaaaatttgt catcttagga tcttcaacga tgaaaaggga gttatgaatc tttccatcat
    11281 gcaagatgga ggtcggttat tggttgtaag ccagtttact ttgtgcgcaa acacagaaaa
    11341 gggacatcgg ccttcgtata tcaaagcggc taagcccgag aaagcaaaag ctatgtatga
    11401 agacttctgt tccttttgcg aaaaaatatt aaacaaaaaa accgaacagg gagtgtttgg
    11461 cgcccatatg ttggttgaac ttatcaacga cggccctgta actattttgc tagattctaa
    11521 aaacagataa taatgacttt gaaggatgca caaattcagg tagacgcttg gattaaacaa
    11581 tacggggtcc gttacttcaa cgaattgacc aatatggcct tattgtgcga agaagtggga
    11641 gaagttgcca gaatcattgc ccgggagtat ggcgaacaat ctaaaaaacc gaacgaaaac
    11701 acagcgaaac ttagcgatga attggccgat gtcttgtggg ttttgatttg tctggctaat
    11761 caaaccggaa ttgatttaga acaagccttt gctgaaaaca taaagaaaaa aaacctacga
    11821 gacaaaagca gacatcagga aaacaaaaaa ttatatgcgt agaaggaggc cttataacct
    11881 cctgattttg agcagataat gttgtgggtt gtgagtttaa atacagcgat tcgccgcata
    11941 ttccaagcat aaattcaagg agtctttagc atctgatgga ggaaattcag cgagtatttt
    12001 ttcggctttc tgtttatatt cttccatttt ttttgataag taatccaaac ctcctgactc
    12061 gatggcaaag cggtacatac gacgaacatt gtactttgta aaatgacgtt tagccataat
    12121 ttcgcgatat ggacgggatt tcttttcatc gctatgtccc agggcataga ttaagggtaa
    12181 cgttactttt ccctctttta aatcgttcgc aaccggtttt ccgggtattt ctccggattg
    12241 accgggtaaa atatagtcga atgaatcgtc ttttagttga aaaataatac ccaaatactc
    12301 tccaaacaag gccaaccggt tgatcgcttc cggttttgca gaagaagcca tggctcctgc
    12361 ttttgcacaa aaggcaaaaa gctgagccgt ttttttatta attatatcaa agtaaattgc
    12421 ttctgtagga ttttgctgct gactaacatg aagttgtact aactcgccat cggccagact
    12481 ggctcccaac tttccaattt ctgttataat atccagtcga cccgttagtg cggcaatttc
    12541 aattactttg gttaaataat aatcgcctga taaaatggcc accttatttt tccaaatggc
    12601 atttatagag ttctttgatc tgcgttggta agtttcatct atcacatcat cgtgtaagag
    12661 gctggcagtg tgcaaaattt gcaaagatgc agcaatattg atcgaagtct gttctaccgg
    12721 accaaacatt gccgatgaaa gcaaaagtac agtcggacgt atttcttttc ccagcgatcc
    12781 gtttaaatac tgtactactt ccgacaatat agggttttca ggttccaggg ctttcttgta
    12841 tgtttcctga tagagaagaa actcctt
//
LOCUS       DAOSGL010000022        12610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4187_length_12610_cov_4.978813, whole genome shotgun sequence.
ACCESSION   DAOSGL010000022 DAOSGL010000000
VERSION     DAOSGL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4187_length_12610_cov_4.978813"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..172
                     /locus_tag="PKY68_01535"
     tRNA            101..172
                     /locus_tag="PKY68_01535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:133..135,aa:Met,seq:cat)
     gene            264..1631
                     /gene="radA"
                     /locus_tag="PKY68_01540"
     CDS             264..1631
                     /gene="radA"
                     /locus_tag="PKY68_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOH23754.1"
                     /translation="MAKAKTIFLCSNCGSDFPKWMGKCPVCGEWGSINEEIIHRDATS
                     KQLTSQFKAEKSQPQLLTEVESNQEARISTGGPELDRVLGGGLVQGSVVLLGGEPGIG
                     KSTLVLQTAIKMTHKRVLYVSGEESLQQIKMRAERIKGDNPRAYFLAETSLENIFEQI
                     SRLKPELLIIDSIQTLSTEVADSSPGSVTQVRECATALLKVAKTLGMPIVLIGHINKE
                     GSLAGPKVLEHIVDTVLLFEGDTHHFYRILRSVKNRFGSIAEIGMYEMRSDGLREVTN
                     PSELLLSQHQDGLSGVAIAAAIEGIRPFLIETQALVSTAAYGTPQRSTTGFDLRRLNM
                     LLAVLEKRAGFKIAQKDVFLNIAGGIRVNDPAIDLSVIAAILSSNLNLSIGNKVCLAG
                     EVGLAGEIRPINRLEQRIVEAQKLGFEQIIVPDFSESVTDLRRFKIRIDKVRKVEESF
                     RLLFG"
     gene            1726..2301
                     /locus_tag="PKY68_01545"
     CDS             1726..2301
                     /locus_tag="PKY68_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOH23755.1"
                     /translation="MKEQRMIGSRIKGMRESKSISIEVFSERSGLSVEQIQLIESNSQ
                     IPSLAPLVKMARALGVRLGTFLDDNEDLGPVVSRKEDQPAGISFSSNTASSHNDLQFF
                     SLAQKKAGRSMEPFIIDIQSSTQNGYALSSHEGEEFIYVLEGEIEIFYGKRVFKLAQG
                     DSIYYDSIVDHNVHAANNSEARILAVVYTPF"
     gene            2303..3946
                     /locus_tag="PKY68_01550"
     CDS             2303..3946
                     /locus_tag="PKY68_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH23756.1"
                     /translation="MELSNRTVGEFLEHWATTTPDREYLIYSDRDLRFTYKQFNQRVD
                     NMAKGLMAIGVRRGSHIGIWATNVPDWLTFLYASAKLGAVLVTVNTNYKQHELEYLVE
                     DSDIETLCITEGVFDGNYVDMVNTMLPELKTSQRGHLQSSRFPKLKNIVYIGQEKYRG
                     MFNTAELLLLGQNIDDDELNEIKRSVNCHDVVNMQYTSGTTGFPKGVMLTHHNIVNNG
                     YFTGEHMAFTSEDKLCVPVPLFHCFGVVLATMNVLTHGCTQVMVERFDPLVVLASVQK
                     ERCTALYGVPTMFIAILDHPMFEMFDVTSLRTGIMAGSLCPVELMRRVTDKLHITHIT
                     SVYGLTESSPGMTQTTVNDSFEIRCTTVGRSYPFTEVKVVDPETGKECPPGVQGEICC
                     RGYLLMKGYYKNPQATAEVIDKDGFLHSGDLGIVDENGYYRITGRIKDMIIRGGENIY
                     PREIEEFLYHLEGIKDVQVAGIPSEKYGEEVGAFIILHEGVSMDEEYVREFCRGKIAR
                     HKIPKYVFFVDSFPMTGSGKIQKFKLKDLGLKLLEKRGD"
     gene            complement(3943..4851)
                     /locus_tag="PKY68_01555"
     CDS             complement(3943..4851)
                     /locus_tag="PKY68_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOH23757.1"
                     /translation="MNHIKNILFLLLLPGLLGACQQKDNYLVADNEILMMEDVSFESR
                     EDLKLKGRAAGAQTYAAGNVTVSESTITDEAVKQGSEATDKKKIIRDGHMTIKVKSAQ
                     AAKTEVDSLLLPFGAYYASENFNNNEREATFYLRLRIPAAAFDDFILCLEKGGYGEIL
                     NKDIQARDVTDQFIDLETRLQNKRNYLSRYKDLLKEAKTVKDILQIQEEIRGLEEEIE
                     STTGRLKYLSDQVDYSSLQLTLTEQKDFQYKPEQRDRFGEKLKQALTKGWYGVVDTVI
                     FLFKIWPVWLILGLALVFLRRRNKPA"
     gene            complement(4848..5855)
                     /locus_tag="PKY68_01560"
     CDS             complement(4848..5855)
                     /locus_tag="PKY68_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH23758.1"
                     /translation="MEYRKIGKTDLELSVITFGAWAAGGMMWGGTKQNDAVNAIRASY
                     DMGVTSIDTAPVYGMGYSEQLVGEAIKGLPRDKVQILTKYGLRWDTTEGEYYFDSVSN
                     EGKTVHIYRNARKENIIQECETSLKLLGTDYIDLYQIHWYDPTTPVGESMEAVSRLME
                     QGKVRYAGVCNYSADQMEEAAAFIDLASDQLPYSMLRRGIEDEVVPYCIKHNKGILAY
                     SPLQRGLLTGKMKPGQQFDPNDSRTGQAWYLDENIVQVNNFLNRIRPIAEAHKASLSQ
                     VVLRWTVEQPGITVALAGARNVAQATENARAGMLKLSPDELRIIDTELSKINLVFPEK
                     K"
     gene            6043..7743
                     /locus_tag="PKY68_01565"
     CDS             6043..7743
                     /locus_tag="PKY68_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOH23759.1"
                     /translation="MKNVVIEYIMKLRLHFTGLILLTFFSIALQAAVPAFPGAEGAGA
                     YTTGGRGGKIYRVSNLNDSGPGSLREAVQARGKRIVIFDVAGIIDLQSTLKITNGDIT
                     IAGQTAPGKGICLRNYPLQVAANNVIIRFIRSRMGEEAGVEGDAIWGRYRQDIIIDHC
                     SFSWSVDECASFYGNKNMTMQWCTICESLNLSVHVKERHGYGGIWGGSPASFHHNLIV
                     HHTSRTPRLSGGDPATEMVDVRNNVFYNWGPTNGGYGGTGGSYNFVGNYYKPGPSTAV
                     KDFLCNRIMYTGPSENPDSAGNYIWGIFHFDDNYFDDSCPDLSETAINNCSRVNANNY
                     VGLHLSTSANLPGGTKYGIQSSTPFSCLPVSTHQPQDAYDKVLELAGASLKRDKQDQR
                     YVAELIDGIYTYEGSRGSTNGIIDSQNDVGGWDTYTGRNTEGDSDGDGMPDNWEDIHG
                     LDPADPLDGPAYTLSQQYTNVEMYLNSLVASIMEAGLEGAESNLRSVDKSDDTPSYLW
                     TEAGLLVPGAVSLELYTMGGLLLAKAQGSLLEISPTPGFFLLRVKDAAFREHSISLVK
                     "
     gene            7763..8227
                     /gene="bcp"
                     /locus_tag="PKY68_01570"
     CDS             7763..8227
                     /gene="bcp"
                     /locus_tag="PKY68_01570"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOH23760.1"
                     /translation="MRMLQKGEKAPDFKGVNQQGEIISLSDFRGKRVILYFYPKDNTP
                     GCTAEACSLNDSRSYFLDKGFVIIGVSPDSVESHQRFAAKYGLSFNLISDPEKAIIQA
                     YGAWGEKKNYGKTYMGLLRTTFVISADGIIEEVFPKVDTKKHAEQIIEALGI"
     gene            complement(8231..10960)
                     /locus_tag="PKY68_01575"
     CDS             complement(8231..10960)
                     /locus_tag="PKY68_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOH23761.1"
                     /translation="MKKHFTNSIWLPALLAVFLSFSITSCKDRPKEKANLAVLAKVQG
                     QRGGAETHPLNDGEIPNELSEQQRNRMMQAVEQANQPQYRRYNQLVSQQLEYRWASPV
                     TIDEVQLYWFNYEGLAKLPQAHRLSYWDGQQFVPVSNAQGYGLENDQFNVTVFDEVTT
                     TALRLELDSLPRYPSTLLEWRVMKSLNSPAVAPMVTAGIDRDVMVGGNTYLNAEIRAV
                     DPVKKLRWSAKGPGKVSFADPKAPTTTASVDKPGKYILTLSAQSGKMKSGSSLELVAH
                     YPPQEERLDVVYTKRYKINSPLWSERAKVLITNWIPWCIDQCERRNLTQGQGGLDNFV
                     EAAKALRGEPHGRHLGYVFSNAWVHQTVESMCIALMVDPQGDKEIIAAQNKMKKALER
                     WIPIILSAQEPDGYLHTAYTLRDTTRWTGRWSPRNRSDHEGYVAGYFIESAINHYTLT
                     EGKDTRLYDAAKKLADCWVKNIGPGPDQIEWYDGHQEMEQALVRFGRFVNDMEGNNKG
                     DSYIALAKFLLDMRDKGGEYDQSHLPVQQQYEAVGHAVRAVYTYSGMSDVAAETGDID
                     YQSAVMSLWNNMVNRKYYVTGGVGSGETSEGFGPNYSLRNNAYCESCSSCGLIFFQHK
                     MNIAYHDAKYADLYEETMYNALLGSIDFEGKRFYYTNPLTAYNARDPWHVCPCCVGNI
                     PRTLLMIPTWTYVTGKDGIYVNMYVGSTINVERVAGTDVEMVQQTDYPWKGEVSIIVN
                     PKEDKHFTVYVRVPNRKTSELYTSVPELNTLKSLSVNGEAVEMKADRGYIAIARDWKK
                     GDRIDLEIPLEVQQITADEKVEALSGLVALRYGPMIYNVERADNKDINQAVSSEPFKA
                     VWRSDMFKGIVGIEGKWADGSDLLAIPNYLRMNRIQPVQEGRRSPESMVWMRK"
     gene            complement(11196..>12610)
                     /locus_tag="PKY68_01580"
     CDS             complement(11196..>12610)
                     /locus_tag="PKY68_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HOH23762.1"
                     /translation="YNWSLNNGGNIIVWDDAKGANQQWYLEYAGDGWFYIRSRHSSHC
                     LQIQNASTANAANIQQGTKNGSANQQWRFLPVGAAIEFNPPAAPAEITATVNSHSLRL
                     DWAAVGDADLAGYTIYRSDSLQGSFQTLARNINGTSFVDHSIEAGKTYYYAVKAIDHS
                     LNHSAFSDTVSAMASGGRDMLAWLSFEDNTLDNTPHLNHGEGLEGLSYVEGINASRAL
                     TLDGTRGFVQLPSTLGNHPELSLALWVNWQGGDSWQRIFDFLYDQTSYFYLTPKIGVF
                     NKMRFAIRNKGGEERLEAAALPKNQWCHVAVTLSDSLACLYVNGEIADQSTQFSLNPA
                     DVRPIINYLGRGQHVSVLFKGQLDDFRVYSYPLSPDEVAALAANGPDALPGISGSEIS
                     ELKIWPLPARDLLYLDCGRDLQGSGQIDLYSLDGNKVATYALKGSKAELDLSHLAAGI
                     YILQLNHGGSSTLRTIIIEP"
BASE COUNT         3043 a         2964 c         3208 g         3395 t
ORIGIN      
        1 caccaagaaa cggattgtgt tttttaatgc tattagtgaa gaaaataccg gtaaaatttg
       61 gatttttatg aaccttggct tatttttgta cccacaaaac tgcggggtgg agcagtggca
      121 gctcgttggg ctcataaccc aaaggtcgca ggttcgagtc ctgtctccgc aactaagtta
      181 gaggggctgt ttgaaaacag cccttttttt cggacattta cctcggacat ttgcccctat
      241 taaattccta aacaatttcg ctcatggcca aagctaaaac catcttttta tgcagcaatt
      301 gcggttccga tttcccgaaa tggatgggga aatgtcctgt ttgcggggag tggggaagta
      361 ttaatgaaga aataattcat cgcgacgcga catcaaaaca actgacttct cagtttaagg
      421 ctgaaaaatc ccaacctcaa cttttgaccg aagttgaatc caatcaagaa gcgcgtataa
      481 gtaccggagg cccggagctg gaccgggtac tgggcggagg cctggttcag gggtctgttg
      541 tcttgttggg gggagaaccc ggcatcggca aatcaactct ggtactccaa acagccatca
      601 agatgacaca taaacgtgtt ttgtatgtgt cgggcgaaga aagtttgcag cagatcaaga
      661 tgcgggctga acggattaaa ggcgataatc cccgggctta ttttcttgca gaaacctctc
      721 tcgaaaatat ttttgagcag atcagccggc ttaaaccaga attactgatt attgattcca
      781 ttcagacctt aagcaccgaa gtggccgatt cttcacccgg cagcgttacc caggtgaggg
      841 aatgtgcaac agcgttgctt aaagttgcca agactttggg catgccgatc gttttgatcg
      901 gtcatatcaa caaagaaggc agcttggcag ggcccaaagt gttggaacat atagtggata
      961 ccgttttgct tttcgaaggc gatactcacc atttttaccg tattttacgc tcagtcaaaa
     1021 accgctttgg tagcattgcc gagatcggta tgtacgaaat gcgcagcgat ggtttgcggg
     1081 aagtcaccaa tccatccgaa cttttattat cacagcatca ggatggtttg agcggggtgg
     1141 ctattgctgc tgccatcgag ggcatccgcc ctttcctgat tgaaactcag gccttggtga
     1201 gtacggcggc ttacggcact ccgcaacgtt ccactaccgg tttcgatctt aggcggctca
     1261 atatgttgtt ggccgtactc gaaaaaagag ccggttttaa aatcgctcaa aaggatgtct
     1321 ttcttaatat tgcagggggc attagagtga acgatccggc cattgatttg tctgtgattg
     1381 ctgccatatt atcgtccaat ttgaaccttt ccatcgggaa taaagtgtgt ttggccggcg
     1441 aggtgggcct ggccggcgag atcaggccga tcaaccgctt ggagcagcgc atcgtggaag
     1501 ctcaaaagtt gggttttgag caaattattg ttccggattt ctctgaatct gtaacagacc
     1561 tgcgccgttt caaaatccgc atagataagg tgcgcaaggt agaagaatcc ttccgcttgc
     1621 tctttggtta agcctttgga gagtgctatg taaaagcttg cggcttattt gatgaaataa
     1681 aaaatagact taattttacc acccaatttt ttaaacaata caagaatgaa agaacagcgg
     1741 atgatcggtt ccaggattaa aggaatgcgc gaatcaaaat caattagtat cgaagtcttt
     1801 tcggaacgtt caggcttgag cgtggagcaa attcagctta tagaaagcaa cagtcagata
     1861 ccttcgctag ctcctctggt taaaatggcc agagcgctgg gggtacgttt ggggaccttt
     1921 ctcgacgaca acgaagacct gggacccgtg gtcagccgca aagaagatca gccggccggc
     1981 ataagtttct cgagcaatac ggcaagttcc cacaacgatt tgcaattctt ctcgctggct
     2041 caaaaaaaag ccggacgcag tatggagcct tttattattg acattcaatc gtccactcaa
     2101 aacggctacg ctctttcttc tcacgaggga gaagaattta tttatgtgct cgagggagag
     2161 atagaaatat tttatggtaa aagagtgttt aagctcgctc agggcgatag catttactac
     2221 gattccatcg tagatcataa tgttcatgct gccaacaatt cagaggcccg gatcctggcc
     2281 gtagtttata cccctttctg atatggaatt aagtaatcgt accgtaggag aattcctgga
     2341 acactgggca acaaccaccc ccgatcgtga gtacctgatc tattccgacc gagacttgcg
     2401 ttttacctac aagcagttta atcaacgagt agataatatg gccaaaggcc tgatggccat
     2461 tggtgtcaga agaggatcgc atataggcat ttgggctacc aatgtccccg attggctgac
     2521 ttttctttat gcttcggcta aactgggagc ggtgctggtc accgtcaaca ccaattataa
     2581 gcagcatgaa ctggaatatc tggtcgaaga ctccgatata gagaccttgt gcatcaccga
     2641 aggtgtgttc gacggcaatt acgtggacat ggtcaatacc atgttgccgg agctcaaaac
     2701 ttcgcagagg gggcatttgc aaagcagtcg tttccctaag ttgaaaaaca tcgtttacat
     2761 cggtcaggaa aaataccggg gtatgttcaa cacggcggaa ttattgttgc tgggccaaaa
     2821 catcgacgac gacgaactga atgaaatcaa gcgcagcgta aactgccacg atgtggtgaa
     2881 tatgcagtat acttcgggaa ctacgggttt tcccaaagga gtaatgctta cgcaccacaa
     2941 cattgtgaac aacggctatt ttaccggcga gcatatggcc tttacatccg aagataagct
     3001 ctgtgtaccg gtgccgcttt tccattgttt cggagtagta ttggccacca tgaatgttct
     3061 gacccacggt tgtactcagg tgatggtgga acgcttcgat ccactggtgg tgctggcatc
     3121 cgttcaaaag gaacgttgca cggctctgta tggagtgccc accatgttta tcgctatttt
     3181 ggatcacccg atgttcgaga tgtttgatgt tacctcgctg cgcacaggca tcatggccgg
     3241 ttccttgtgt ccggttgaat tgatgcgcag agtaaccgac aagctgcata tcacccatat
     3301 caccagcgtg tacggactta ccgagagttc gccgggcatg acccagacca cagtaaacga
     3361 ttctttcgaa atacgctgta ccaccgttgg tcgcagttac ccttttaccg aagttaaagt
     3421 ggtagatccc gaaaccggca aggaatgtcc tcccggtgta cagggtgaga tttgttgcag
     3481 aggttatctg ttgatgaagg gttactacaa aaatcctcag gctacggcgg aagtaatcga
     3541 caaggacggc ttcctgcact cgggcgattt gggaatagtc gatgaaaacg gttattatcg
     3601 cattaccggc cgtattaagg atatgattat ccgtggcggt gaaaatattt atcctcgtga
     3661 aattgaggag tttctctatc acctcgaagg gatcaaagac gttcaggtgg ccggcattcc
     3721 ttccgaaaaa tacggcgaag aagtcggagc gttcattatt ctgcacgaag gtgtcagtat
     3781 ggacgaagag tatgtcaggg aattttgccg tggcaagatt gcccgtcata agattcccaa
     3841 atacgtcttt tttgtcgaca gcttcccaat gaccggcagc ggtaagatcc aaaagtttaa
     3901 actcaaggat ctgggtttaa aactgctcga aaagcggggt gattaagcag gtttgttacg
     3961 tcttctcagg aaaaccaggg caaggccgag gattaaccag accggccaga ttttaaagag
     4021 gaaaatcaca gtgtccacta ctccatacca acctttggtg agagcttgtt tcagtttctc
     4081 accaaagcgg tcgcgttgtt cgggtttgta ttggaaatct ttttgctcgg tgagcgttag
     4141 ttgcagactg ctgtaatcca cttgatcgct taggtatttg agccggccgg tggtgctttc
     4201 gatttcttct tcgagccccc ggatttcttc ctgaatctgc aggatatctt taacggtttt
     4261 ggcctctttg agtaagtcct tgtaacgact caagtaattg cgtttgtttt gtagacgggt
     4321 ttccagatcg ataaactggt cggttacatc ccgggcttga atgtctttgt tgagtatttc
     4381 tccataacca cccttttcga ggcagagtat aaaatcatca aaagcggcag cgggaatccg
     4441 caacctcagg taaaaagtgg cctcccgttc gttgttgttg aagttttcgc tggcgtaata
     4501 agccccaaat ggaagcaaaa ggctgtccac ttcggttttg gctgcctgag ccgattttac
     4561 cttgatggtc atgtggccgt ctcgaatgat tttcttcttg tctgttgctt ccgatccttg
     4621 ttttacagcc tcatcggtga tagtgctttc agaaaccgta acgttgcctg ccgcgtaggt
     4681 ctgagcgcct gccgcacgcc ctttgagctt gagatcttct cttgattcaa aggaaacgtc
     4741 ttccatcatt aatatttcgt tatctgcaac aaggtagttg tctttttgtt gacatgctcc
     4801 aagcagtccc ggcaacagga gcagaaaaag aatgtttttt atatgattca tttcttttcg
     4861 gggaatacca agttgatttt gcttaattcg gtatcgataa tacgaagttc gtcggggctg
     4921 agtttcagca tgcctgcccg ggcgttttcg gtagcctggg ctacgttcct ggcgccggcc
     4981 aaagcaacgg tgatgcccgg ctgttcaacg gtccagcgca atactacctg actcaggctg
     5041 gccttgtgcg cttcggcaat ggggcggatc ctgttgagga agttgttgac ctgcacgatg
     5101 ttttcgtcca gataccaggc ctggccagtg cggctgtcgt tgggatcaaa ttgctggccg
     5161 ggtttcattt tgccggtaag caatccccgt tgcaaaggac tgtaagccaa aatacccttg
     5221 ttatgcttaa tgcaataagg tacaacttca tcttctatcc cccggcgcag catgctgtac
     5281 ggcagttgat ccgatgccag gtcgataaag gcagccgctt cttccatttg gtcggcagaa
     5341 taattgcaaa caccggcata acgcaccttg ccttgttcca tgaggcggct gacggcttcc
     5401 atgctttcgc ccacgggagt ggtgggatcg taccagtgta tctgatagag atctatgtaa
     5461 tcggtgccca gcaatttaag gctggtttcg cattcctgaa taatattctc tttacgggca
     5521 ttgcggtaaa tatgcactgt tttgccctcg ttgctgacgc tgtcaaaata gtactcgcct
     5581 tcggttgtat cccaacggag cccgtattta gtcaggattt gtactttgtc gcgaggaaga
     5641 cccttgatgg cttctcccac cagttgttcg ctgtagccca tgccgtaaac gggagccgtg
     5701 tctatgctgg ttacgcccat atcgtaagaa gcgcggatgg cgttgaccgc atcgttttgt
     5761 ttggtgcctc cccacatcat acccccggcg gcccaggctc caaaagtaat aaccgagagt
     5821 tccagatctg ttttcccaat ttttctgtat tccatagtga ttttgttttg ttgccctcaa
     5881 agatataatt ttattccggc ctatcattat ctttgtaaag tatgaaagat tttgcagcca
     5941 tcgatttcga tctgaggcat cattatgctt tggccgatgt agaagcttgt gcggcgatag
     6001 agttgagatt attcaaacca ctctgaataa atagactaaa tcatgaaaaa cgttgttatt
     6061 gaatatatta tgaagctcag actacatttt accgggctga tattacttac ctttttttct
     6121 atagcgcttc aagctgccgt gccggctttt cccggagccg agggagccgg tgcttatacc
     6181 accggaggcc ggggaggaaa gatttaccgg gttagcaatt taaacgattc cggcccgggt
     6241 tctcttcgcg aagccgtgca ggccagaggt aaacgcatcg ttatttttga tgtggcgggc
     6301 atcattgatt tgcaatcgac cctgaaaatc actaacggag acattactat tgccggccag
     6361 acagcacccg gcaaaggaat atgtttgaga aattatcccc tgcaggtagc tgccaataac
     6421 gtaattatcc gctttattcg cagccggatg ggagaagagg ccggggtgga aggcgatgct
     6481 atttggggac gttaccggca agacattatc atcgatcact gctcattcag ctggagcgtg
     6541 gatgaatgcg cttcttttta tggaaacaaa aatatgacca tgcaatggtg taccatttgc
     6601 gaaagtctca atctgtcggt gcatgtaaaa gaaaggcacg gttacggggg tatctggggt
     6661 ggttcgccgg ccagtttcca tcacaatctg atagtgcacc acaccagccg tacgcccaga
     6721 ctcagcggag gcgatcccgc cactgagatg gtggatgtgc gcaacaacgt tttttacaac
     6781 tggggcccca ccaacggagg ctacggagga acaggcggca gctacaattt tgtgggtaat
     6841 tactataaac ccggccccag cacggcggtc aaggattttc tctgcaatcg tataatgtac
     6901 accgggcctt cggaaaaccc ggattcagcg gggaattata tttggggcat tttccatttt
     6961 gacgataatt atttcgacga cagctgtccc gatctcagcg aaacagccat caataattgt
     7021 tcgagggtga atgccaataa ttacgttggt ttacatcttt ctaccagcgc caatctaccc
     7081 ggagggacta aatacggcat acagtcgtcc actccctttt cctgtttgcc cgtgagcacc
     7141 catcagcccc aggatgccta cgacaaggtg ctggaattgg ccggagccag tctgaagcgt
     7201 gataagcaag atcagcgcta tgtagccgaa ttgatcgacg gcatctatac ttacgaaggc
     7261 agcaggggca gcaccaacgg cattatagat tcacaaaacg atgtgggtgg ctgggatacc
     7321 tataccggaa ggaacaccga aggcgattcc gacggcgacg gcatgcccga caactgggaa
     7381 gacattcacg gtctggatcc tgccgatccc ttggatggtc cggcttacac tttgtcgcaa
     7441 cagtacacca atgtagaaat gtatttaaac agtctggttg cttctattat ggaagccggt
     7501 ctcgaggggg ccgagtcgaa tctacgatcg gttgataagt ccgatgacac cccttcctac
     7561 ctatggaccg aagccggtct gttggttccc ggggctgtca gcctggaact ttatacgatg
     7621 ggtggcctgc tgctcgccaa ggcgcaggga agtttactgg aaatctctcc cacacccggc
     7681 tttttcctct taagggttaa agatgctgct ttccgggagc atagcatcag tctggtaaaa
     7741 tgaaacataa ccaaattaaa aaatcagaat gttacaaaaa ggcgaaaaag ctcccgactt
     7801 caagggagtg aatcaacaag gcgaaatcat ttccctgagt gatttcaggg ggaaaagagt
     7861 gatactctac ttttatccca aagacaacac ccccggctgc acggccgaag cgtgcagcct
     7921 taacgacagc aggtcgtatt ttttggataa aggctttgtt attatcgggg tcagtcccga
     7981 ctcagtggag tcgcatcagc gatttgccgc caaatacggc ctgtcattca acctgatttc
     8041 cgatcccgaa aaagccatta ttcaggcata cggtgcctgg ggcgaaaaga agaactacgg
     8101 caaaacctat atgggccttc tgcgtaccac ctttgttatt tcggccgatg gcattataga
     8161 agaggtgttc cccaaggtcg ataccaaaaa acacgccgag caaatcatcg aggcgttggg
     8221 aatttaaatc ctatttccgc atccaaacca tcgactcagg actgcgtcgt ccttcttgta
     8281 cgggctggat tctgttcatc ctaagataat taggtatagc cagcaagtct gagccgtcgg
     8341 cccatttgcc ttcgataccg actatgcctt tgaacatatc gctgcgccag acagctttaa
     8401 aaggctcgct gcttacggct tggttgatgt ctttgttgtc ggctctttcc acattgtaaa
     8461 tcatcggccc gtagcgaaga gcaaccagcc cgcttagagc ttcaaccttt tcgtcggccg
     8521 tgatttgctg cacttccaga ggaatttcca ggtctatgcg atcgcccttt ttccagtcgc
     8581 gggcaatggc tatataacct ctgtctgcct tcatttccac agcttcgccg tttacactga
     8641 gcgatttgag cgtattcagt tcgggtaccg aagtatacag ctcactggtt ttgcgattgg
     8701 gtacccgcac ataaaccgta aaatgtttgt cttctttggg attgacgatg atagaaactt
     8761 cgcctttcca gggataatcg gtttgctgta ccatttcaac atctgtgccg gctacccttt
     8821 ctacattgat ggtgctgcct acgtacatat taacgtagat accgtctttt ccggtaacgt
     8881 aagtccaggt gggaatcatc agcagggtgc gggggatatt acccacgcag caggggcaga
     8941 catgccaggg atcacgcgcg ttataggccg taagcggatt ggtgtaataa aaacgcttac
     9001 cttcgaaatc gatcgaaccc aataaggcat tgtacatagt ttcttcgtag agatccgcgt
     9061 atttggcgtc gtgatatgcg atgttcattt tgtgctggaa aaatatcagc ccgcagctcg
     9121 agcaagattc gcaataggcg ttgttacgca gagagtagtt ggggccaaag ccttcggaag
     9181 tttcgccgct gcccacaccg ccggtaacat aatattttct gttgaccata ttgttccaca
     9241 aagacattac ggcgctttga taatcgatat cgccggtttc ggcggccaca tccgacatgc
     9301 ccgagtaggt gtagacagct cgtacagcgt gtccaacggc ttcgtattgc tgttgtacgg
     9361 ggaggtggct ttgatcgtat tcgccgcctt tatcgcgcat gtcgagcagg aatttggcca
     9421 gggcaatgta gctgtcgcct ttattgttgc cttccatgtc gttaacaaaa cggccaaagc
     9481 gcaccagggc ctgttccatt tcctgatgtc catcgtacca ttcaatctga tcgggacccg
     9541 ggccaatgtt ctttacccaa cagtcggcca gttttttggc agcatcgtac aaacgagtgt
     9601 ctttgccttc ggtcagggtg tagtggttaa tggcggattc tataaaatag ccggccacat
     9661 agccttcgtg atcgctccgg ttacgcggag accagcggcc ggtccagcgg gtggtgtcgc
     9721 gcaaagtgta ggctgtgtgc aggtagccgt cgggttcctg agccgataaa atgatgggaa
     9781 tccacctttc cagggctttc ttcatcttgt tttgggcggc aatgatttct ttgtcaccct
     9841 ggggatctac catcagggcg atgcacatag attccaccgt ttgatgaacc caggcattgg
     9901 aaaacacata acccagatgt cggccgtgag gctctccgcg taaggctttg gccgcttcga
     9961 caaagttgtc aagcccgccc tgtccctgag taagattgcg gcgttcgcat tggtcaatgc
    10021 accagggaat ccagttggtg attaatactt tggctctttc gctccaaagg gggctgttga
    10081 ttttgtaacg tttggtgtag accacatcca aacgttcttc ctgaggagga taatgtgcaa
    10141 caagttccag gcttgatccg gatttcattt ttcccgattg ggcagagagc gtcagaatgt
    10201 atttgccggg cttatccacg ctggctgtgg tggtaggagc ttttgggtcg gcaaaagata
    10261 ccttgcccgg tcctttggcg ctccaacgca gctttttaac cggatcgacc gccctgattt
    10321 cggcattcag gtaggtatta ccgcctacca ttacgtcacg gtctataccg gccgtaacca
    10381 taggggcaac agccggagag ttgagcgatt tcattactct ccattcaagt aaagtgctcg
    10441 gatagcgagg cagagagtcc aattccagcc gcagcgcggt agtggtgact tcgtcaaaaa
    10501 cggttacatt gaactgatcg ttctccagtc cgtaaccttg tgcattgctg acgggtacaa
    10561 attgctgtcc gtcccagtaa ctgagccggt gggcctgagg caatttggcc agtccttcgt
    10621 aattgaacca ataaagttgc acctcgtcta tggtgaccgg gctggcccaa cgatattcca
    10681 attgctggct cactaattgg ttgtagcgtc tgtattgggg ttgattggct tgttcaacgg
    10741 cttgcatcat ccggttccgt tgctgttcgc tcagttcgtt agggatttca ccatcgttga
    10801 gcggatgcgt ttctgcaccg cccctttgac cttgaacctt ggccaatacg gccaggttag
    10861 ctttttcttt gggtctgtct ttgcaggagg ttatgctaaa gcttaagaac actgccagca
    10921 gggcaggcag ccaaatggag tttgtgaaat gttttttcat agttgtgaat ttttatagag
    10981 tgatttattt aagtcagaag aacgcccggt attttgaagg cgttgataat taatcattac
    11041 gcaagttaca tgcttttatc taataagttc tgcgttttgt atgcagaaat tcctaaaatc
    11101 ttctcaaagt ataacactat agtccaagta ataaggagtt tagtgcataa tccggtttat
    11161 ctacagaaaa ctcagatgtg atatattgcc cattattagg gctcaattat aattgttctt
    11221 aacgtactgc ttccgccgtg gttgagctgt agaatataaa tacctgctgc cagatggctg
    11281 agatcgagtt cggccttgct gccctttaag gcgtaagtag ccactttgtt gccgtcgagg
    11341 ctgtagaggt cgatttgacc gcttccctgg agatcgcgtc cgcagtcgag ataaaggagg
    11401 tcacgggcgg gcagaggcca tattttcagt tcgcttattt cgctgccgga aatccccggt
    11461 agggcgtccg ggccatttgc cgccagtgcg gctacttcat cgggcgagag agggtagctg
    11521 tagaccctaa agtcgtccag ttgtcctttg aacaagacac tcacgtgctg gcccctgccc
    11581 aggtagttga ttatcggccg gacatccgcc ggattcaggc tgaattgggt cgattgatct
    11641 gctatttctc cgtttacata gaggcaagcc aggctgtcgc tcagggtaac ggccacatgg
    11701 caccattgat tctttggcag cgcggcagct tccagtcttt cttcgcctcc tttgtttcgg
    11761 atggcaaaac gcattttatt gaaaacgcct atcttggggg tcaggtaaaa atagctggtt
    11821 tgatcgtaaa gaaaatcgaa aatacgttgc cagctatctc ccccttgcca gtttacccat
    11881 aaagccaggc tgagctccgg gtggttgccc agagtggacg gcagttgcac aaaaccccgg
    11941 gttccgtcaa gggtcagtgc cctggaggca ttgatgccct ccacataact caggccttcg
    12001 agtccttcgc cgtgattcag atgtggagta ttgtctaagg tgttgtcttc gaagctgagc
    12061 catgcaagca tgtccctgcc gccgcttgcc atcgctgata ctgtatcgga aaaggcggaa
    12121 tgattcagcg aatggtctat cgctttaaca gcgtaataat aggtcttgcc cgcttcgatg
    12181 gaatgatcca caaaggaagt gccgttgatg tttctggcca aagtctgaaa cgatccctgc
    12241 aaggaatcgg agcgataaat ggtgtaaccg gccaggtctg cgtccccgac ggcagcccaa
    12301 tcgagacgca aggaatgaga atttacggtg gcagttattt ctgcaggggc ggcaggcgga
    12361 ttaaattcaa tggctgcgcc caccggcagg aagcgccatt gctggttggc tgagccgttt
    12421 ttggtgccct gttgaatgtt ggcggcattg gcagtggagg cgttttgtat ttgcaaacaa
    12481 tgcgagctgt gtcggctgcg gatgtaaaac cagccgtcgc cggcgtattc caggtaccac
    12541 tgttggttgg cacctttggc atcgtcccaa acgatgatgt tgcccccgtt attgagagac
    12601 cagttgtaaa
//
LOCUS       DAOSGL010000023        12537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4215_length_12537_cov_5.161993, whole genome shotgun sequence.
ACCESSION   DAOSGL010000023 DAOSGL010000000
VERSION     DAOSGL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4215_length_12537_cov_5.161993"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PKY68_01585"
     CDS             <1..1206
                     /locus_tag="PKY68_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOH23763.1"
                     /translation="FTRPHAQPIPSGKEASIDDIVWMGPQSSLAEKALKSGITKSFDI
                     DAIYDILNKAKAQDRTIHFLPPYRADNKLWLSKALNLPDEVLNDLASLSLIKAIVQQR
                     EIKSEEEIEEIEKAVDTSVDMHVAAMKTARPGMSEAQVAAEVHRIALAQGGNISFPII
                     ATINGQTLHNHYHGNILKEGDLFLLDAGAETARHYSGDLSSTFPAGKTFTPLQKTIYE
                     AGLKAHRAAVNSLAPGRRFRDVHRAACLSIIDSMIALGLMKGNPADAFEQGAHALFFP
                     CGTGHQMGLDVHDMEDLGELWVGYDGEPKSTQFGLKSLRFAKELKPGHVFTIEPGIYF
                     IPELIDLWKAEKKHPDFLCWHEIEKFRNFGGIRNEEDFLITENGYRLLGNKKKPMTVG
                     EVEAYRSAR"
     gene            1309..1382
                     /locus_tag="PKY68_01590"
     tRNA            1309..1382
                     /locus_tag="PKY68_01590"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1343..1345,aa:Arg,seq:tct)
     gene            1705..1896
                     /locus_tag="PKY68_01595"
     CDS             1705..1896
                     /locus_tag="PKY68_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOH23764.1"
                     /translation="MYNGKVKFFNEAKGFGFIKETETSKEYFVHASGLIDRVKENDEV
                     TFELEEGRKGLNAVNVKLV"
     gene            complement(1975..4311)
                     /locus_tag="PKY68_01600"
     CDS             complement(1975..4311)
                     /locus_tag="PKY68_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOH23765.1"
                     /translation="MRAKSLFYVLISLLFFSCNTLKYVPEDRQLLTGTSIELKDEKLP
                     KKELKNYLRQQPNSRFWGLFRINLGLYNISGQDTSRWINRSLRKIGEAPVVYEELLTQ
                     RSAMALERYLFSKGYFDSEVETRTMSKGRKIKTIYSIRPNQAYRINSYTVEVDPRDPF
                     HMLIYDALPQSAVQIGDKFDTNLLDEERSRLVSWARNYGYFAVNKDHFSFEADSSLNK
                     NLVDIRLIIKPYLRRSREGVSQYVPHKQYRIYRVYFMLDVPSSVYLRNLGIQGTSNFF
                     MALSDYDSIPYENYAVVYRDKPFIKPKVLVDNCMINPGDYYDARAVNRTYARFNSMQN
                     MKYVNIRFEEITAQDSSSGGEAQLDCYVVLTPGKNNIISMDVEGTNTAGDLGVAGTLT
                     YSHLNLLRGGETFSTSLRGAYEALSSSFQNDYTEFGGEIGLQFPDFKIPFLSSDFRKR
                     VDANTNFKISYDNLSRPEFLRTMASSAVEYVWRNQRLRQNLSLLDLAYVYMPRVDSAF
                     KATYLTDNSYLKYSYEDHFILRTQYGILYSSVPFGVSNRTFFTIRANIESAGNVLRLA
                     YSLLEAEKGENQAYTIGKIPFSQYLKGEFEYARSVVLNPRNRFAYRAGMGVAYPYGNS
                     KVLPFEKRYFSGGANSVRGWSVRTLGPGTYKNSLNTIDFMNQSGDLKLDINAELRSKL
                     FWVLEGGLFVDVGNIWTLRNYDNQPGGQFQFDEFYKQLACSAGLGLRFDFNFFLVRVD
                     AGIKVFDPSGLTVDDRWRIKHIDRWDDFALHLAIGYPF"
     gene            4429..5187
                     /locus_tag="PKY68_01605"
     CDS             4429..5187
                     /locus_tag="PKY68_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOH23766.1"
                     /translation="MLSKAKIKWIHSLNLKKNRKELHLFVAEGHKLVTELLPLMSCRF
                     LTATAEWLQKYPDIRAAEIAEASLQELNKASNQQHPQEVLAVFEIPDRKPDLNYLKNN
                     LTLALDNIQDPGNLGTIVRTADWFGINTLLCSVGCADAYNPKSVQASMGSIARVAVHY
                     VKLEEFIPTLNVPVFGTFLEGKNLYEFPHLPQAAVILMGNESKGISGKLKPLVNEKLY
                     IPKFPPGSKRAESLNVSIATGIICAAFRRSQTNS"
     gene            complement(5177..5971)
                     /locus_tag="PKY68_01610"
     CDS             complement(5177..5971)
                     /locus_tag="PKY68_01610"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOH23767.1"
                     /translation="MNSIKDILTKVLLLGLSVLVLTACRQEEGKVLSQKKMAAVLTDI
                     YLTEAVLQNVDRKKKTEWSRGLEDVFFQDLAYGRVLEKHRITEEVFYNSVAHYSQQYK
                     VYAKIYEQVELNLQAIRDEVDRRDKLETKLRELAQQQAALVEALDTASYIKWFEIWAL
                     DTIVLSDTLGLQADSVVLSDTLGLQTDSAVLLDTLVSQTGDVLLSPLNDSILYTISFW
                     PKPVVRDRNEWTMADFTPGMESGKVLREDRNPVTVDLKRTIREVKN"
     gene            complement(5982..6638)
                     /locus_tag="PKY68_01615"
     CDS             complement(5982..6638)
                     /locus_tag="PKY68_01615"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOH23768.1"
                     /translation="MAKPSKVWKAFVLIAIILVVDQAVKIYIKTHFQLHESFRVTNWF
                     YIYFTENSGMAFGWEFFDKIFLTLFRIIAAGLLIWYMVYISRKKQFPTGYVLSIALII
                     AGALGNVIDCVFYGLIFDHSYGQVAGFFPEGGGYAPIFYGKVVDMLFFPLINTTLPDW
                     LPIWGGRDFLFFRPVFNIADTSITTGILILLIFYRSCLAEDTKGKKSKSDKAGSPLTT
                     "
     gene            complement(6642..7019)
                     /locus_tag="PKY68_01620"
     CDS             complement(6642..7019)
                     /locus_tag="PKY68_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOH23769.1"
                     /translation="MSEIIRYSDAELEEFRQLIQKKLDKARNEYEQLKASINNIDGND
                     VMDTSPIFKVFEEGANTLLKEETGRLAQRQMKFIENLEAALVRIDNKTYGICRETGKL
                     IPKGRLRAVPHATLSIEAKNVKS"
     gene            complement(7117..10545)
                     /gene="ileS"
                     /locus_tag="PKY68_01625"
     CDS             complement(7117..10545)
                     /gene="ileS"
                     /locus_tag="PKY68_01625"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOH23770.1"
                     /translation="MSKKFAEYTEFDLSAINKSMLEKWESTDLFQKSTALREGGPSFV
                     FYEGPPSANGMPGIHHVMARTIKDIFCRYRTMKGFEVKRKAGWDTHGLPVELGVEKAL
                     GITKEDIGKKISVIDYNAACRKDVMKYTREWEDLTRKMGYWVDMSDPYITYDNRYIET
                     LWYLLKQLYQKGLLYKGYTIQPYSPAAGSGLSTHELNQPGCYRDVKDTTCVAQFKIKN
                     PRPEWSQHGEAFFLAWTTTPWTLPSNTALCVGPAISYAAVQSYNFYTGQPATYILARD
                     LLTAHFSPKAAELPLDQYKAGEKNIPYKVLEQDYKGEELLGIQYEQLIPWVNPGGKAF
                     EVISGDFVSTDDGTGIVHIAPTFGADDQFVAKKFNISPLLLVDKEGNTRPMVDTGGKF
                     FRLEDLDPAFIESNVNVEKYAPYAGRYVKNEYMPEGSETEDNLDVDLCVMLKQENRVF
                     KIEKQVHNYPHCWRTDKPILYYPMDSWFIKTTACRDQMIALNRTILWKPASTGTGRFG
                     KWLENLQDWNLSRSRFWGTPLPIWRTEDGSEELCVGSVAELYEEIEKAVARGFMKENP
                     YKDFVPGLYTAENYSVEKIDLHRPYVDEIVLVSPSGKAMKREPDLIDVWFDSGAMPYA
                     QIHYPFENKEAFDSGQCYPADFIAEGVDQTRGWFFTLHALAAMLFDSVAFKSVVSNGL
                     VLDKNGNKMSKRLGNAVDPFVTIEKYGSDPLRWYMITNASPWDNLKFDEEGIEEVRRK
                     FFGTLYNTYSFFVLYANVDGFDNQAAQIPLQERPEIDRWILSQLNSLIRDTDQYYNDY
                     EATRSGRAIADFVGENLSNWYVRLNRKRFWGGGYSKDKLSAYQTLYTCLETVSRLMAP
                     IAPFYADRLFLDLNVVAKGERCESVHLADYPQCNYDAIDSALENRMQMAQQISSMVLA
                     LRRKVNTRVRQPLQTIMIPALDAAQKAAIEAVRELILNEVNVKELKFIDTAGGLLIKR
                     IKPDFKKLGPRCGKNMKQVANMLGEMQQDAIAEFEQKGYCTIKLEGMDLRIEAGDVEI
                     LSEDIPGWLVANEGRFTVALDVKITEELRREGLARELVNRIQNLRKTQNFEITDRIHI
                     LIEKNPSFAGTLEDWGEYISTQTLALSLQQVDVLEGGTILDMDDISLNIKILKA"
     gene            complement(10611..11348)
                     /locus_tag="PKY68_01630"
     CDS             complement(10611..11348)
                     /locus_tag="PKY68_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOH23771.1"
                     /translation="MSSSSFRFKQFEVFHDRCAMKVGLDGVLLGAWAKPPADLSEAAR
                     LRILDVGSGSGLIALMLAQRFPSSLVFGIEKDIQAALQAQENVARSPWPERIHIIQGS
                     FPESINDNFSPEEQQFDLIVSNPPYHRNALKAKAYTRNLARHGDKLSFEQLTISAAGL
                     LSEKGVFSLIVPAEAAELIEELCWGQRLYPTAICRISHRQGKEPKRSLMAFSRFRADI
                     CRQELQIEDNKGEYTAEFKALTSDFYL"
     gene            complement(11350..11721)
                     /locus_tag="PKY68_01635"
     CDS             complement(11350..11721)
                     /locus_tag="PKY68_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23772.1"
                     /translation="MKKFFCWNKMSKDTLNNLLFTLAAVLILISSVLVMEHQAWARFS
                     FATGVVLYIAHRALTAYKGDDFRLKRLNRMFAFNGIVLILAVYLMFRNSNSWVAILLI
                     VAVIELYTAFRSASYQKENEQ"
     gene            complement(11696..11995)
                     /locus_tag="PKY68_01640"
     CDS             complement(11696..11995)
                     /locus_tag="PKY68_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOH23773.1"
                     /translation="MRKILAFYEKIKPYFNKYVIVLILFGVLIVFIDEHNLIRRMRYD
                     SKIRSLRKEIRRYERERDRALEQLEDLRTGSEELEKIARERYLMKKDDEEIFLLE"
     gene            12136..>12537
                     /locus_tag="PKY68_01645"
     CDS             12136..>12537
                     /locus_tag="PKY68_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH23774.1"
                     /translation="MENYLVSARKYRPDNFKSVVGQSALTTTLQNAIKNKQLAHAYLF
                     CGPRGVGKTTCARIFAKSINCFHPNNQGEACNKCESCVSFNEQRSYNIHELDAASNNS
                     VDDIRSLTEQVRIPPQLGKYKVYIIDEVHMLS"
BASE COUNT         3508 a         2969 c         2645 g         3415 t
ORIGIN      
        1 tttacccgtc ctcatgcgca gccgatcccg tccggcaaag aggcgagcat cgacgatatt
       61 gtatggatgg ggcctcaaag cagtcttgcc gaaaaagcgc ttaaatcggg cattacaaaa
      121 agctttgata tcgatgctat ttacgacatt cttaacaaag caaaagctca ggacagaacc
      181 atccattttc ttcctcctta ccgtgccgat aataaacttt ggctatccaa ggctttaaat
      241 ttgccggatg aagtattgaa cgacctagcc tccctgagtt taatcaaagc catagttcaa
      301 caacgcgaaa tcaaatctga agaggaaatt gaagagatag aaaaagcagt ggacacttcg
      361 gtggatatgc acgttgctgc tatgaaaaca gccaggccag gaatgagcga agcccaggtg
      421 gctgccgaag tacaccggat tgcacttgca cagggcggga atatttcttt ccctatcatt
      481 gccaccataa acggccagac tttacataac cattaccacg gcaatatcct aaaggaaggc
      541 gacttgtttt tgctcgatgc aggcgctgaa acggccaggc attattcggg agatctttcc
      601 agcacttttc cagccggcaa aacgttcaca cctctccaaa agacgatcta tgaagccggt
      661 ttgaaagctc accgtgctgc cgtgaacagc ctggctcccg gcaggagatt tcgggacgtt
      721 caccgggctg cttgcttgtc gatcattgac agcatgatcg ctttgggctt aatgaaggga
      781 aatcctgcag atgcctttga gcagggagct catgccttgt ttttcccctg cggcaccggg
      841 catcagatgg gcctggatgt gcacgatatg gaagatttgg gcgaattatg ggtgggttac
      901 gacggcgaac ccaaaagtac tcagtttggc ctaaaatcgc tgcgttttgc caaggaacta
      961 aaaccaggac acgtttttac catcgaaccc ggcatctact ttattcccga gctcattgac
     1021 ttgtggaaag cagaaaaaaa acaccccgat tttttatgct ggcacgaaat tgagaaattt
     1081 agaaacttcg gtggcatccg caatgaagaa gattttctta taaccgaaaa cggctatcgc
     1141 ttgctgggta ataagaaaaa accaatgacc gtcggcgaag tagaggctta ccgctccgcc
     1201 cgctgaggag tcttaggcat tccggaacct tgaacattcc agtgtacgga actcaaaata
     1261 agccttgcaa attagcatgt tttaatttac ctttgccggc gcaaaaaagg accggtagct
     1321 cagctggata gagcaacagc cttctaagct gtaggtcttg ggttcgaatc ccaaccggtt
     1381 cacttttgat tgatacatgt caaaaccact cagcaaacgt tttacattaa ttagttaagt
     1441 ctctcttctt tagagctatg tctgcctgac ctccgcttgc gaaatactac actctttgca
     1501 acaaaacacc tccttcgcgc cttttgagtg tcctgaatcc aagttctcca tacgcttatt
     1561 cagtgctaaa acagtgtaat tgtacaaaaa ccggctgttt ttatataatt acctgacatg
     1621 cagaaaaatg aacaaaaaaa acactgcttt tgaaaattat taaattttag tccttatctt
     1681 tcgcagtcaa aatttttaaa cagtatgtac aacggaaaag taaagttttt taacgaggcc
     1741 aaaggtttcg ggtttatcaa agaaacggaa acttcaaaag agtatttcgt gcacgccagt
     1801 ggtcttattg accgcgtcaa agagaatgat gaagtcactt tcgaactgga agaaggcaga
     1861 aaaggattaa atgcagtaaa tgttaaattg gtataacatc ttaacataca ccatttagta
     1921 taattgagaa aagctgtgtt tccctgggaa cacagctttt tttatacaca acttctagaa
     1981 aggatagcct atggccaagt gtagagcaaa gtcgtcccat cgatcgatat gcttaattct
     2041 ccatcgatca tctacggtaa gccccgaagg atcaaacact tttatgcccg catccacacg
     2101 aaccaaaaag aaattaaaat cgaaacgcag gcccagaccc gcactgcaag ccaattgttt
     2161 ataaaactca tcgaattgga actgaccacc cggttgattg tcgtagttgc gtaaagtcca
     2221 gatgttgccg acatccacaa acaaaccgcc ctcaagtacc caaaataatt tactgcgcaa
     2281 ttccgcatta atgtccagct taaggtctcc ggactgattc atgaaatcga tcgtattaag
     2341 gctgttttta taggttcccg gccccagggt tctgacagac catccacgta cggagttggc
     2401 tcctccggaa aaataacgtt tttcaaacgg caagaccttt gaattaccat aagggtaagc
     2461 cacccccata cccgcacgat aagcaaaacg gttacgggga ttcaaaacca cgcttctcgc
     2521 atattcaaat tctcctttca ggtattgtga aaagggaatt tttcctatcg tataagcttg
     2581 attttcgcct ttttctgcct ccagcaagga gtaagccagt cttaatacat tgcctgccga
     2641 ttcaatattg gcacgtatgg taaaaaaggt ccggttagag accccgaaag ggacgctgga
     2701 ataaagaatt ccatattggg ttcgcaatat aaaatgatct tcgtaactgt attttaaata
     2761 cgaattatcg gtgaggtagg tcgctttgaa agcagagtcc acccggggca tgtaaacata
     2821 ggccagatcg agcaaactca aattctgacg caaacgttga tttctccaga catattcgac
     2881 ggcgctggaa gccatggttc ttaaaaattc cggacgggac aggttatcgt aactgatttt
     2941 gaaattagtg ttggcgtcca ctctctttct gaaatcactg gacaaaaagg gaattttgaa
     3001 gtccggaaac tgcaggccga tttcgccccc aaactctgta tagtcgttct gaaaagaact
     3061 cgacaaagcc tcataggctc cgcgtaaact tgtactaaaa gtttctccgc ctctaagcaa
     3121 attaaggtgt gaataagtta gtgttcccgc aacacccaga tcgcccgctg tgttggtgcc
     3181 ttccacatcc atagaaatta tattgttctt gccgggagtc aataccacat aacaatccag
     3241 ttgagcttca ccacctgaac ttgaatcttg tgcggtaatt tcttcaaaac gaatattgac
     3301 atatttcata ttctgcatgg agttgaagcg ggcgtaagtt ctgttcacag ctcgtgcatc
     3361 gtaataatcg ccgggattaa tcatgcaatt atctaccaac actttaggtt taataaaggg
     3421 cttgtcgcgg tatacgacgg cgtaattctc gtagggtatg gaatcgtaat ccgatagcgc
     3481 catgaaaaaa ttggaagttc cctggatacc taagtttcgc aaatagaccg aactgggcac
     3541 atccaacatg aaataaaccc ggtaaatacg gtattgtttg tggggcacat attgagaaac
     3601 gccctctcgg ctgcgccgca aataaggctt gattatcagc cttatatcaa caaggttctt
     3661 gttgaggctg ctgtctgctt caaaactgaa atgatcttta ttgacagcaa aatagccata
     3721 gtttctggcc caggaaacca gccgggaacg ctcttcgtcg agcaggttgg tatcgaactt
     3781 gtctcctatt tgcaccgcag actgaggcaa agcatcgtaa ataagcatat ggaaaggatc
     3841 ccggggatcg acttctaccg tgtagctgtt gattcggtag gcctgattgg gacgaatgct
     3901 gtaaatagtt ttgatcttcc ttcctttgct cattgtccgg gtttctactt ctgaatcaaa
     3961 gtaacccttg ctgaacaaat agcgctccaa agccatagcg gaacgttgag tgagcaattc
     4021 ttcgtaaacc accggggctt ctccgatctt tctcaggcta cgattgatcc atctgcttgt
     4081 gtcctgaccg gatatgttgt ataatcccag attgattctg aacaagcccc agaaacgcga
     4141 gtttggctgt tgtcgcaggt aattctttaa ctcttttttt ggaagcttct cgtctttgag
     4201 ctctatgctt gttcctgtaa gcaattgcct gtcttccggc acatatttaa gtgtattgca
     4261 ggaaaaaaac aacagggaaa tcagcacata aaacagactt tttgctcgca ttccggattt
     4321 tcttattgcg gcaaatgtac atcattccaa gcaatgttca caaattcaca tagataaatt
     4381 tctgcaggct gaatttcttt tgtagttttg tttttctaaa aaaaacttat gctgagtaaa
     4441 gccaaaatca agtggataca ttcgttgaat cttaaaaaaa acagaaaaga actccatttg
     4501 tttgtagccg aaggccataa attggtaaca gaactactgc ctcttatgtc ctgccgcttt
     4561 ttgacagcca cagcagaatg gctgcaaaaa tatccggaca ttcgggctgc tgaaatagcg
     4621 gaagcctctt tacaggaact caacaaagcc agtaatcaac aacaccctca agaagtactg
     4681 gccgttttcg aaatacccga cagaaaaccg gatttaaatt atttaaaaaa taacctaacg
     4741 ctcgccctcg acaatattca ggacccgggc aacctgggta ccatcgttcg cactgccgac
     4801 tggtttggca tcaatacgct gctttgttcc gtaggatgcg cagatgctta taacccgaaa
     4861 tccgtacagg ccagcatggg ctccatagcc agggttgcgg ttcattatgt taaactcgaa
     4921 gaatttatcc ccacattgaa cgttcctgtt ttcggtacct tcctcgaagg aaaaaatctt
     4981 tatgagttcc cgcatctgcc acaagcggcc gtaatactga tggggaacga aagcaaagga
     5041 atttccggca aactcaaacc tcttgtaaac gaaaaactgt atatccctaa attcccgccg
     5101 ggcagcaaaa gggcagaatc gctcaatgtc tccattgcta caggaatcat ttgtgctgct
     5161 ttcagaagat cgcaaactaa ttcttgactt ccctaatcgt tcttttcaaa tctaccgtaa
     5221 ctggatttct atcctcccgg agtacttttc ccgactccat accgggcgta aaatctgcca
     5281 ttgtccactc gtttctgtcc cggacaacag gtttcggcca aaaagaaata gtatataaaa
     5341 ttgaatcgtt caagggggat aaaagcacat cacctgtttg tgaaaccagg gtatccaata
     5401 aaaccgccga gtctgtttgc aagcccaaag tatccgataa gacaaccgag tctgcctgca
     5461 agcccagtgt atctgatagg acaattgtgt ctaatgccca aatctcaaac cacttgatat
     5521 aagaagccgt gtcgagagct tcgacaagag ctgcctgttg ttgggccaac tctcgtaatt
     5581 ttgtctctaa cttgtcgcgc cggtccacct catcccgaat agcctgcaaa ttaagttcaa
     5641 cctgttcata tatctttgca taaaccttgt actgctggct gtaatgcgcc acagaattat
     5701 aaaaaacctc ttcggttata cggtgtttct ctaataccct tccataggcc agatcctgaa
     5761 aaaaaacatc ctcgagcccc cgggaccatt ctgtcttttt cttcctgtct acgttctgca
     5821 acacggcttc cgtgagataa atatccgtca gtacggcagc catctttttt tgtgaaagga
     5881 ctttgccctc ttcttgtctg caagccgtca gcaccagaac agacaatcca agaagtaata
     5941 ctttggtcag tatgtcttta atgctattca taagagggtg gtcaggtcgt gagaggagaa
     6001 cctgccttat cgctcttgct ttttttccct tttgtatcct cggcgaggca tgagcggtaa
     6061 aagatcaaca gtatcaggat acctgtagtg attgaggtat cggcaatatt gaaaacaggc
     6121 ctgaaaaaca aaaagtcccg gccaccccaa atgggcagcc aatcgggcaa agtggtgttt
     6181 atcaacggga aaaacagcat atcaaccacc ttgccgtaaa aaatcggagc gtaaccaccg
     6241 ccttcgggga aaaatccggc tacctgtcca taactgtgat caaaaatcaa accataaaac
     6301 acacaatcga tcacattgcc caaggctccc gctataataa gtgctatact caaaacatag
     6361 cctgtgggga attgtttttt acggcttatg tacaccatgt accatatcag caagccggcg
     6421 gcaattattc gaaacaaggt caggaatatt ttgtcaaaaa actcccagcc aaaggccatt
     6481 ccactgtttt cggtaaagta gatataaaac caattcgtca cacgaaaact ttcgtgcaac
     6541 tggaaatgcg tttttatata tatcttaacc gcttgatcta caaccagtat gatggcaatt
     6601 agaacaaacg ctttccatac tttggaaggt ttggccatcc tttaagactt gacgttttta
     6661 gcttcaatac tcagcgttgc gtgagggacc gcacgtaaac gccctttagg gatgagtttt
     6721 ccggtttccc tgcaaatgcc gtaagttttg ttatctatac gaaccaaagc tgcttccaga
     6781 ttttcgatga atttcatctg acgttgggcc agacgtccgg tttcttcttt caacaaagta
     6841 ttggcgcctt cttcaaaaac cttgaaaatg ggagaagtgt ccattacgtc gttgccatct
     6901 atgttgttga tgctcgcttt taattgttca tactcattcc tggccttgtc taattttttc
     6961 tgaattagct gacgaaactc ttccaattct gcatctgaat aacgaataat ttctgacatg
     7021 gtattttgta ttatatggtt agatttttaa tctgcgaata tagcaaaaat aaacatcgac
     7081 agcaatagaa aaacgatctc tgctgtttta aaaaaatcag gcttttaaaa ttttgatatt
     7141 taatgatata tcgtccatat ccaaaatcgt tcctccttca agcacgtcca cttgttgcag
     7201 acttaaagcc aaagtctgag tagagatgta ttctccccaa tcttccaagg ttccggcaaa
     7261 ggaaggattc ttttcaataa ggatatgaat gcggtcggtt atctcgaaat tttgagtttt
     7321 gcgcaagttc tgaatacggt tcaccaattc acgagccaaa ccttcccttc tcaattcctc
     7381 ggtgatttta acatcaaggg ccacggtaaa ccgcccttcg ttagctacca gccaaccggg
     7441 gatatcttca gaaagaattt ccacatcgcc tgcctcaata cgcaggtcca tgccttcaag
     7501 ttttattgtg caatagccct tttgttcaaa ttctgcaatg gcgtcttgtt gcatttctcc
     7561 caacatgttt gccacctgct tcatgttttt cccacatctg ggcccaagtt tcttaaaatc
     7621 gggtttaatg cgcttgatca gcagtccgcc ggcagtatct ataaacttca attctttaac
     7681 gtttacttcg tttagaataa gctcccggac agcttcgatg gctgctttct gggctgcgtc
     7741 caaagcaggg atcataatgg tttgcagcgg ttgccttacc ctggtattta ccttgcgtct
     7801 aagggccagc accatggacg aaatttgttg agccatttgc atccggttct ccagggcact
     7861 gtctattgcg tcgtaattgc attgcggata gtcggccaga tgaacagatt cacatctctc
     7921 tccctttgca acgacattta gatccaaaaa cagccggtct gcgtaaaaag gagcaatggg
     7981 agccatcagc cgggatacgg tttccagaca agtgtacaag gtctggtaag ccgaaagttt
     8041 gtctttggaa tatcctccgc cccagaagcg cttgcggttg aggcggacat accagttact
     8101 taaattttca cccacgaagt ctgctattgc gcgtcccgat ctggtggctt cgtagtcgtt
     8161 atagtactga tctgtatcgc gaatcaagct attcaactgc gaaagtatcc aacgatctat
     8221 ttcgggcctt tcttgcagcg gaatttgtgc tgcctgattg tcgaaaccat ctacattagc
     8281 gtataaaaca aagaaagaat acgtattgta gagagtgccg aaaaatttcc gtctgacttc
     8341 ttctatgcct tcttcatcga attttaaatt atcccaggga gaagcattag taatcatata
     8401 ccaacgcaaa ggatcggaac cgtatttttc gatggtaacg aaaggatcca cggcattacc
     8461 cagacgtttt gacattttgt tgccgttctt atccagaacc aaaccattgg aaaccacgct
     8521 tttaaaagca acagaatcaa agagcatagc cgcaagggcg tgcagggtga aaaaccatcc
     8581 gcgagtctga tctactccct cggcaataaa atcggcagga taacattgac ccgagtcgaa
     8641 ggcttctttg ttttcgaagg gataatgaat ctgggcgtaa ggcatggctc cggaatcgaa
     8701 ccagacgtca atcaggtcag gctcacgctt catggctttg cccgagggag aaaccaacac
     8761 tatttcatcc acataaggac gatgcaggtc tattttttcg accgaataat tttcggccgt
     8821 gtacagaccg ggaacaaaat ccttgtaggg attttctttc ataaatccac gggccactgc
     8881 cttctctatc tcttcgtaca gctcggccac cgaaccgaca caaagttctt cgctaccgtc
     8941 ttcggtacgc catatgggca gaggtgtacc ccagaagcga ctgcgtgaca ggttccaatc
     9001 ctgcagattc tcaagccact tgccaaagcg gccggtaccg gtagaggccg gtttccacaa
     9061 gatggtccga ttgagggcaa tcatttgatc gcggcaggcg gtcgtcttta taaaccagct
     9121 gtccatggga taatacagga ttggcttgtc ggtacgccag cagtgaggat aattgtgcac
     9181 ctgcttttcg attttaaaaa cacggttctc ctgtttgagc atcacacaga ggtccacatc
     9241 cagattatcc tcggtttcgg agccttccgg catgtattcg ttcttaacat aacggcctgc
     9301 gtatggcgcg tatttttcta cattaacatt gctctcgata aaggccggat caagatcttc
     9361 caacctgaaa aatttcccgc cggtgtctac catgggacgt gtattgccct ctttatcgac
     9421 cagcaaaaga ggacttatat tgaatttttt ggcaacaaac tggtcgtctg ctccaaaagt
     9481 aggagcaatg tgtacaatac ctgtaccgtc gtcggtggag acaaagtcac cgctgattac
     9541 ttcaaaagcc ttccctcccg gattgaccca gggaattaac tgctcgtatt gtatacccag
     9601 taattcttct cctttgtaat cctgttccag caccttgtaa ggaatgttct tttctcccgc
     9661 cttatattgg tccaaaggca attcggctgc ttttgggctg aaatgggcag tcagcaaatc
     9721 tctggccaga atataggtgg ccggctgtcc ggtataaaaa ttatagctct gcacagccgc
     9781 atagctgata gccgggccca cacaaagagc cgtattggag ggcaaagtcc agggagtggt
     9841 ggtccaggcc aggaaaaagg cttctccgtg ttggctccat tccggacgtg ggttttttat
     9901 cttgaactgg gccacacagg ttgtgtcttt gacgtccctg tagcagcccg gctgattcaa
     9961 ttcatgggtg ctcaggcccg aaccggcagc aggagagtaa ggctggatgg tataaccctt
    10021 gtagagcaaa cccttttggt ataattgctt gagcaagtac cacaaagttt cgatatagcg
    10081 attgtcgtag gtgatgtaag gatcagacat atcaacccaa taacccatct tacgggtaag
    10141 gtcttcccac tcgcgggtgt atttcatcac atccttacgg caagcggcat tgtagtcgat
    10201 gacggaaatt tttttgccta tatcttcttt ggtaattccc agggcttttt ctactcccaa
    10261 ttccacaggg aggccgtgag tatcccaacc ggctttgcgc ttaacctcaa agcccttcat
    10321 ggttctgtaa cggcaaaata tatctttgat ggtcctggcc attacatggt gtattccggg
    10381 catgccattg gccgagggag gtccttcgta aaaaacaaaa gacggccctc cttctctcaa
    10441 agccgtactt ttctggaaaa gatcggttga ttcccatttt tctaacatcg atttgttgat
    10501 ggccgacaaa tcgaattctg tatactcagc aaattttttt gacatcttgc tatcgttatc
    10561 ctaattaagg gcgcaaaggt acattaaaaa aatatactat gaaaaaggat ttaaaggtaa
    10621 aaatccgagg ttaaggcctt gaattcagca gtatattcac ctttattgtc ttcgatttgc
    10681 agttcctgac gacaaatatc cgcccggaac ctggaaaagg ccatcaggga acgcttgggt
    10741 tctttgccct gacggtgtga aatccggcag atagctgttg ggtaaagcct ctgcccccag
    10801 cagagttctt ctatcaattc tgcagcttcg gctggtacaa taagagaaaa aactcctttc
    10861 tcgctcaaca agccggcagc acttatagtc aattgttcaa aactcaattt gtcgccgtgg
    10921 cgggctaaat tacgcgtata agctttggcc tttagggcat ttctatgata aggaggattg
    10981 gaaacgatca gatcgaactg ttgctcttcg ggactaaaat tgtcgttgat gctttcgggg
    11041 aaagagccct gaatgatatg tattctttcg ggccagggac tacgggcaac attttcctgt
    11101 gcctgcaatg ccgcctgaat gtctttttcg ataccaaaaa ccaaggaaga gggaaaacgt
    11161 tgagccaaca tcagcgcaat caagccggaa ccgctgccca catccagtat cctcaatctt
    11221 gccgcttcgg acaaatcggc ggggggttta gcccatgcac ccaacaacac cccgtccaga
    11281 cccaccttca tggcgcaacg gtcgtgaaaa acctcaaatt gcttaaaacg gaaagaggaa
    11341 gaggacatgc tattgctcgt tttctttctg ataagaagct gaacgaaaag ccgtgtacaa
    11401 ttcgatcaca gccactatca gcaagatcgc cacccaggaa ttgctgtttc tgaacatcag
    11461 gtagacggca agtataagaa ctatgccatt gaaagcaaac attcggttaa gtcgctttag
    11521 tctgaaatcg tcgcctttgt aggccgtgag ggcacgatgt gcaatataga ggactactcc
    11581 ggttgcaaaa ctaaatctgg cccaagcctg atgttccatt accaaaacgg acgaaatcag
    11641 gatcagcacc gcggccagtg taaacagtag attatttaga gtgtctttgg acattttatt
    11701 ccagcaaaaa aatttcttca tcgtcttttt tcatcaaata gcgttcacgg gcaattttct
    11761 ccagttcttc actgccggtt ctcaaatcct caagctgttc cagagcccgg tctctttccc
    11821 tttcgtatcg tcgtatttct ttacgcaaag agcgtatttt actgtcgtat cgcatacgtc
    11881 tgatcagatt gtgttcatcg atgaacacaa tcagcacacc aaacaaaatc aacacgatca
    11941 cgtatttatt aaaatacggc ttaatctttt cgtaaaacgc caaaatcttg cgcataggcg
    12001 gatttatgaa cagaacaaaa ttacgagaat aatccggatg cccaagtgtt ttccaaatca
    12061 aaaatcgttg ccggaacttc ctttttaaaa aagccttatg tatctttgtc cacctaagcc
    12121 agtagcgtaa ttagtatgga aaattatttg gtttcagcac ggaaataccg tccggacaac
    12181 tttaaatcgg tcgttggaca aagcgctctc acaacaactc tccagaatgc catcaagaac
    12241 aagcaactgg ctcatgccta tctattttgc ggccccagag gagtaggcaa aaccacctgt
    12301 gccaggattt ttgccaaatc catcaattgt tttcatccca acaatcaggg ggaagcctgc
    12361 aataaatgcg aatcttgcgt ttctttcaac gagcagcgct catacaatat tcacgaactg
    12421 gatgccgcct ccaacaactc ggttgacgac atccgttctc tgaccgaaca agtgcgcatt
    12481 ccgccacaac tgggcaaata caaggtttac attatcgacg aagtgcacat gctctct
//
LOCUS       DAOSGL010000024        12440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4268_length_12440_cov_4.636738, whole genome shotgun sequence.
ACCESSION   DAOSGL010000024 DAOSGL010000000
VERSION     DAOSGL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12440
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4268_length_12440_cov_4.636738"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKY68_01650"
     CDS             <1..350
                     /locus_tag="PKY68_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23775.1"
                     /translation="YANLSSYVPSEHLNFSWVRLSPVTCVLIFNECIFASQPSMVSIV
                     VIHKNEAKLIFNKPLFIHSISKSTAESFLNITLQSNTLEYGGTPESPVALGIPSLHTI
                     WWDGNVLRYIKQR"
     gene            complement(423..1397)
                     /locus_tag="PKY68_01655"
     CDS             complement(423..1397)
                     /locus_tag="PKY68_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23776.1"
                     /translation="MKKGILFIALGLFAYTMLSCDPVNGNGDGGDDPEAPKNYWERSA
                     LTHAQLRGPVKMVKEFYSDTTYKMTTFDEIGRILKIENTGQGNDFLTNYTYNTSGQLI
                     QAGTTTFEYADHGKYVPRGHFHMNFAGLAPNLSAINDERARTSYTFVGDTLFIITTVQ
                     YSDNADENTYVDTTKILYSGKYPVSSRTEHIFSEGNSWGEFMNASYQENGMFDVYSEG
                     FFGTGDNAYTSTRTYTYKKDDEFMLIDNYRYEDVYPGQEQPSSYTSNYTYNTQKDLTQ
                     IAEGEYIQEYSDYVYDTHNNWTSRKYRYKNGPEWSSYTDERREITYWE"
     gene            complement(1437..3623)
                     /locus_tag="PKY68_01660"
     CDS             complement(1437..3623)
                     /locus_tag="PKY68_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH23777.1"
                     /translation="MKNIVHILLLTMFFASSSQLSASQYLEGNKVPSANNNDLLTHGL
                     FRPNGSTSVYFQLIQENMLRSSNPEMPEPAYLNLYEPDLNLIKSFALRLPVEEAQLNY
                     WAGGLELVNSLVNDTLKQFLIYKFSVYDSAYVSQFTDENWSVFVGVLDESGDSLFTVL
                     SNFHGFETFEVNGQVKLAVNRVCSKDTAFFGGGSWFTTDVYTGQVYDLSTGALELQMP
                     HGIMYEGITFDEGQRRILGRNNNIAAAYEHLYKEYDLLLYNLDGSLYKTAVDSLSDYW
                     VGEFYHLSSVGQYCMKEKYSNTIYSLPDYEEWVSGGIFYDSQGLAKLVDYDNDTVRIY
                     NEDRSLYKSLAFEQDNMHSVVWVNMHDIVSDDKIEIVRECPNGGLQIFTEDGDTIFEN
                     WDLKLVENNDFSGGKVVLRFPDENYGRLIATDMNDYDYFTSYYKVGNLPVIAQMGDAP
                     GNDNIELLGLGEEGFIVLDTLELIDGRGAFVVGEGEYALRSQGKVQSETLRSLSTYLP
                     SELLWENAGIVSFTTDEIDALIISIQTVDLSVSAGASGQIRGTIRLIDNEGGLRSASL
                     PTYDLYLVASSDHSQVKSFTTSNQEGVYLFDRIPAGSYDVLLNVEGKAMTSVNTVVLS
                     SDASIVENVDYEITGEGIVATMQSSIERVRPKAPGSYLIHQGNTLLISGIEGTIQVRI
                     IDLSGRVLLNAQTNRGQVDVSGLQSGIYLLELQAGEQKQVLKFRKR"
     gene            3797..4972
                     /locus_tag="PKY68_01665"
     CDS             3797..4972
                     /locus_tag="PKY68_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOH23778.1"
                     /translation="MLKTILLLTPVYVTLFWTLVLKTANRHNNDRARLFLGKFMFFAC
                     ILYFSHFLFFYPLPQVYIYFDALYQYASLMVYPLFYIYIRLLTVDKKFELKKHYKYIA
                     VPNLLLIAYLIGILFSPYEHYRALIADYENSPWNFLKIVLICMQLTFLIQVILCVTGN
                     TILLKKYKHSAGQYYSDIEDSRIYKAELLNLCIILTGISSFILGILGRDFFFAEPVAM
                     GIASLVFSTLLFAIGLLGNVQKVVNPSYETDSGVQPAANFASDKEMEHFKAEVSPKIL
                     RLFNEDKIYLNSKLTIRDVAQIVGTNRTYVSNFINTHFNQNFCSFVNRYRLEELERTI
                     RKHPEYTGNTLAELCGFGSTDSLKRAVRTSFGMSYSEWRKSLSVQADKHQDNLNAKA"
     gene            complement(4945..5361)
                     /locus_tag="PKY68_01670"
     CDS             complement(4945..5361)
                     /locus_tag="PKY68_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOH23779.1"
                     /translation="MSIDEVQDDIIQAFDEIEDWMDKYQLLIDMGNSLSPLDEQYKKP
                     QYLIEGCQSRVWIHADYKEGKVFFEAESDTVIVKGLVYLLLKVLSGRTPDEILKTDLY
                     FIERIGLKEHLSPTRSNGFAAMLKQIRNYALAFKLS"
     gene            complement(5371..>5748)
                     /locus_tag="PKY68_01675"
     CDS             complement(5371..>5748)
                     /locus_tag="PKY68_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOH23780.1"
                     /translation="GTAALAEALRFIQEIGLDKIADYEKQLVNYALQELSKVEGFQLL
                     GNIPERHAIIPFQLKGIHAYDVGLLLDKLGIAVRTGHHCAQPLMERLAVDSCVRASFA
                     FYNTSSEVDRLQAALKKIYKMFN"
     assembly_gap    5749..5758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5759..6623)
                     /locus_tag="PKY68_01680"
     CDS             complement(<5759..6623)
                     /locus_tag="PKY68_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOH23781.1"
                     /translation="MESKFDVASIRKHFPILEQKAYGHPLVYLDNAATTQKPRSVVKK
                     IEEAYFSHNANVHRAVHLLSQQATEAHEAARVVVQQFINAAEPDEIVFTRGTTESINL
                     VAFSFGEAFCREGDEILISSMEHHSNIVPWQLLCERKKMTLKVIPIDDNGCLLVDEYE
                     KLLGPKVKLLAITHVSNVLGIENPIKQIIEKAHKQGVPVLVDGAQAVAHMPVDVRALD
                     ADFYTFSSHKVFGPTGVGVLYAKRSFLEQMPPYQGGGEMIDQVHFEKTTYNRIPFKFE
                     AGTQNVEGAVGL"
     gene            complement(6640..7977)
                     /gene="sufD"
                     /locus_tag="PKY68_01685"
     CDS             complement(6640..7977)
                     /gene="sufD"
                     /locus_tag="PKY68_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOH23782.1"
                     /translation="MGKETTYLNLFRNYREVLRQYSAPLMDSKREAAIGLFEKAGFPG
                     PKDEAYRQCRLDALLEIDYGMNLYRLSAPAHPSKVFRCEVPSLSTKLFFVVNDVYYPD
                     PDPQKLSEGVLYGSINAILREYPELMAPYYDRIAGQSADAFAALNTAMAQDGFLLFVP
                     AGVEIEQPLQLIQLFHGEIEQMANRRNLVIIEDGASAKLMICDHSMTQSSCFSNQVTE
                     VFVGKKARLEYYELEMTHPNNARLSNTFIDQQASSSVFVNGITLNNGVSRNNLNLRML
                     GEQAESMLSGLCLLDSHQFADYHVDVEHVVPQCTSRQLYKYILDDESRGVFAGKVLVH
                     PNAQKTSAYQSNANLCNSSRARMNALPQLEIYADDVKCSHGSATGQIDENALFYMRSR
                     GLSEQEARAMLKFAFVADVIEGIQLIPLQNRIHNLVGKRFRKQMSACAGCDIC"
     gene            complement(7979..8731)
                     /gene="sufC"
                     /locus_tag="PKY68_01690"
     CDS             complement(7979..8731)
                     /gene="sufC"
                     /locus_tag="PKY68_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOH23783.1"
                     /translation="MLEIKNLHASAGDKEILKGLNLSVKEGEIHAIMGPNGSGKSTLS
                     AVLSGNPAFTVTQGTVRFKGMDLLSMTPEDRSREGLFLSFQYPVEIPGVSMVNFMRTA
                     INEHRKYRKQEAMNASDFLKLMREKKALVELDNTLAGRSVNEGFSGGEKKRNEIFQMA
                     MLDPVLSILDETDSGLDIDALRIVAQGVNKIHTPQKSCIVITHYQRLLNYIVPDRVHV
                     LYEGRIVKSGDFQLAKDLEKQGYDWVKQELKD"
     gene            complement(8787..9137)
                     /locus_tag="PKY68_01695"
     CDS             complement(8787..9137)
                     /locus_tag="PKY68_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23784.1"
                     /translation="MKSKRETKTAQNRGTDDNEAPIQKSRGAIWVAIFILLILAGLGY
                     LLFSTLPLEEEGNRQRLLVSALALMLLVVGLLVFVQGWFKKTTRKKGKAKRKPSRYSP
                     APKSVKRGKKTGRR"
     gene            complement(9100..9777)
                     /gene="pnuC"
                     /locus_tag="PKY68_01700"
     CDS             complement(9100..9777)
                     /gene="pnuC"
                     /locus_tag="PKY68_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOH23785.1"
                     /translation="MSIQDVVLFSLWGHSMNLLELIGLVSGLVAVYLASRALSVNFLF
                     GLLNNVVYFVVFYQAHLYSAMLLQVVYFSFSLYGYYHWKHPAPGQEGKNRELRIRRLN
                     KSQWALVIVIVLVFGGLWSEGIIYFQSRFPEYIDPPAFPRLDAMLTAASVMGQFLLSR
                     KVKDNWIIWIVVDVLSTALYAYMGLFFTALLFAVFTLIALRAIVDWNKQYKQQDEIKK
                     GNKNRTK"
     gene            complement(9779..11230)
                     /gene="sufB"
                     /locus_tag="PKY68_01705"
     CDS             complement(9779..11230)
                     /gene="sufB"
                     /locus_tag="PKY68_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOH23786.1"
                     /translation="MKSTEDKDIITQVTAGEYKYGFVTDIETETIPEGLNEDVIRLIS
                     SKKMEPDWLLEFRLKAFRHWQSMEKPQWAHLDIPEIDYQKMVYYAAPKSKTSGDNEED
                     IDPDLLKTFEKLGIPLQERKQLAGMAVDAVMDSVSVKTTFKESLAKKGIIFCSFSEGV
                     QEHPELIKRYLGSVVPYTDNYYAALNCAVFSDGSFVYIPKGVRCPMELSTYFRINAIN
                     TGQFERTLIVADDDSYVSYLEGCTAPIRDENQLHVAIVEIVVHDRAEVKYSTVQNWYP
                     GDKEGKGGIYNFVTKRCLCKGESAKISWTQVETGSAITWKYPSCILAGDNSVGEFYSV
                     AVTNNHQQADTGTKMIHLGKNTRSIIVSKGISAGKSQNSYRGLVKIAPKASNARNHSQ
                     CDSLLLGDTCGAHTFPYIEINNETAVVEHEATTSKINEDQLFYCNQRGISTEEAIGLI
                     VNGYAKEVINKLPMEFAVEAQKLLQVSLEGSVG"
     gene            complement(11234..11779)
                     /locus_tag="PKY68_01710"
     CDS             complement(11234..11779)
                     /locus_tag="PKY68_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOH23787.1"
                     /translation="MNLYDIIILIVALLGLIDGLRRGLIQTIGTIAGVVCGIFAAKAL
                     SYLVAPYISQLFESASLKVVQVVSFIVLFVLTVILFHFVAVLLGKLVKMVMLGWLNRI
                     AGGLFAVVLYLFVLSIVVNVFDYFDKGKSSFHREKSSTSRFYGPLSDFAPAVLPFIHF
                     EDWNTKKPETLQQSTPVPQSI"
     gene            complement(11783..>12440)
                     /locus_tag="PKY68_01715"
     CDS             complement(11783..>12440)
                     /locus_tag="PKY68_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOH23788.1"
                     /translation="ITLDEIGRRIALGQFKELNIILKGDVDGSVEALSDSFSKLSTEE
                     IQINIIHKGVGAITETDVMLASASDAIIVGFQVRPSMAARKMAERDGVDIRLYSIIYD
                     AIEEIKSAMEGMLSPEIREEVTATAEVREVFKITKIGTVAGCMVKEGKIKRGTKIRLI
                     RDGIVVFTGELSSLKRFKEDVKEVASGYECGLSINNYNDIKVGDLIEAYEEIEVKQTL
                     "
BASE COUNT         3435 a         2959 c         2456 g         3580 t
ORIGIN      
        1 gatatgccaa tctatcctct tatgtgccat cagaacattt aaacttttct tgggttagat
       61 tgtctcctgt gacatgtgtt ttgatcttta atgagtgtat attcgcgagc caaccatcga
      121 tggtgagtat tgtggtaata cataaaaacg aggctaaatt aatatttaac aagcctctgt
      181 ttatccattc tatttcaaaa agtacggctg aatctttttt aaatatcaca cttcagtcga
      241 acactctcga atacggagga acaccggaat cgcccgtagc gctggggatt ccttcgcttc
      301 acacaatttg gtgggatgga aacgttttac gttacattaa acagcgttag ctgtgaactt
      361 cgataagttc acagctaacg ccggcactta aaagtatctg ctaacagatc aaaggaagat
      421 tcttattccc aataagtaat ttcgcgacgc tcatcggtat aactgctcca ctcaggtccg
      481 tttttataac ggtatttacg actggtccag ttgttgtgag tatcgtatac gtaatcggaa
      541 tattcttgga tatactcacc ttcggctatc tgtgtcaggt ctttttgtgt attgtaagta
      601 taattacttg tgtaggagct tggttgttct tgaccgggat aaacatcctc ataccggtaa
      661 ttgtcaatca gcatgaattc atcgtctttc ttgtaagtgt aagttcttgt acttgtatag
      721 gcgttatcac cggtcccaaa gaaaccttcg gaatagacat caaacattcc gttctcctga
      781 taactggcat tcataaattc tccccaggaa ttaccttcag aaaatatatg ttctgttctg
      841 gaagacactg ggtattttcc ggaataaaga attttggtag tatccacata ggtgttttca
      901 tcggcgttgt cactgtattg gacagtggtg ataataaata gggtatctcc cacaaacgta
      961 taacttgtgc gggctctctc atcattaatt gcggaaaggt taggagccaa accggcaaaa
     1021 ttcatatgga aatgacccct tggaacatac ttgccgtggt cggcatattc gaaagtagta
     1081 gttccggcct gaatcaattg tccggaggta ttgtacgtat aattggtcaa aaaatcattg
     1141 ccctgccctg tgttttcaat cttaagaatt cttccgattt catcgaatgt ggtcattttg
     1201 taagtggtgt ctgaataaaa ctctttgacc attttcacag gccctctcaa ttgggcatgc
     1261 gtaagggctg aacgctccca ataatttttg ggagcttcgg gatcatcccc tccgtccccg
     1321 tttccattta ccggatcgca agatagcatc gtataagcaa ataagcctaa agctataaac
     1381 aatattcctt tcttcatgat taaaaattta attataattg aatactaatt ttgatattat
     1441 cgttttctga atttgagtac ttgtttttgc tctcccgctt gtagttcgag cagataaatg
     1501 ccgctttgaa gcccgctaac atccacctga ccccggttcg tctgggcatt gagtaatacc
     1561 ctgccgctca agtcgataat tcttacttga attgtccctt caataccgct tatcaacaag
     1621 gtattgccct gatgtattaa atagcttccc ggtgcttttg gacggactct ttctatgctg
     1681 ctttgcatcg tagccacaat gccttctccg gtaatttcgt aatccacgtt ttctacgatg
     1741 ctggcatcgg acgataagac tacggtattt accgaagtca tggctttacc ctccacattc
     1801 aacaacacat cgtaggaacc ggcaggtatg cgatcaaaaa gataaacccc ctcttggtta
     1861 ctggtggtaa aagatttcac ctgactatgg tctgaagatg ccaccaaata caaatcatag
     1921 gtcggtaaag acgcactgcg caaaccgcct tcattatcga tcagacggat tgttcctctg
     1981 atttgcccgc tggcgcctgc gctgacagat aaatcaaccg tctgaatgct tataattaat
     2041 gcatctatct cgtccgttgt gaacgacact ataccggcat tctcccaaag taattcgctt
     2101 gggagatagg tactcaaact gcgtagagtt tccgactgta ctttcccctg actgcgcaag
     2161 gcatattctc cttctccaac cacaaacgct ccccggccgt cgatcaattc caaagtatcg
     2221 agtacaataa atccttcctc gcccaagcct aataattcaa tgttatcgtt ccccggagca
     2281 tcacccattt gggcaataac gggaagattt ccaactttat aatagctcgt gaaataatcg
     2341 taatcattca tatcggttgc aatgagccgg ccataatttt catccggaaa acggagtacg
     2401 actttacccc cggaaaaatc attgttttca accagtttta aatcccaatt ttcgaagatc
     2461 gtatctccgt cttctgtgaa tatttgcaga cccccgttgg gacattcgcg cacaatttca
     2521 attttatcgt cacttacaat gtcgtgcata tttacccaaa caacactgtg catattgtcc
     2581 tgttcaaacg ccaggctctt gtacaggcta cgatcttcgt tataaattcg gacagtgtcg
     2641 ttgtcgtaat ctaccagttt ggccagaccc tggctatcgt aaaaaatccc gccggaaacc
     2701 cattcttcgt aatcgggcag ggagtatatg gtgttagagt atttctcctt catgcagtat
     2761 tgtccgaccg aagataaatg ataaaattct ccgacccagt aatccgacag actgtcaacg
     2821 gccgttttat ataatgagcc gtccaaattg tataaaagca aatcgtattc tttatacaaa
     2881 tgctcgtagg cagcggcaat gttgttgttt ctgcccaaaa tacgcctttg tccttcgtca
     2941 aaggtgatgc cttcgtacat tattccgtgc ggcatttgca attcgagagc tccggtggat
     3001 aaatcgtata cctgcccggt gtaaacatcg gtggtaaacc agcttcctcc cccgaaaaaa
     3061 gcagtgtctt tggaacagac tcggttcaca gccaatttga cctgcccatt tacttcgaat
     3121 gtttcaaagc cgtgaaaatt acttagaacg gtaaataacg aatctccact ttcatcgagc
     3181 acaccgacaa agacgctcca gttttcgtct gtaaattgcg aaacatacgc actgtcgtat
     3241 acagaaaact tataaatcaa aaactgcttt aatgtgtcgt tgaccaggct attaaccagc
     3301 tcaaggcctc cggcccagta attcaattgt gcctcctcaa caggcagtct caatgcaaaa
     3361 ctcttaatca ggttaaggtc aggctcatat aagttgaggt aagcaggctc cggcatttcg
     3421 ggatttgaac tacgtaacat gttttcctgt atcaactgaa aataaacaga cgtagagccg
     3481 ttggggcgaa ataggccatg cgtcaataaa tcgttgttat tggccgaggg cactttgttg
     3541 ccttccagat actgagatgc ggaaagctga ctgctgcttg caaaaaacat tgtcagcagc
     3601 aaaatatgta ctatattttt cataaagccg tattaaagta tgattaaaat tggattcttc
     3661 ctaagagtct cttttttcat tgaaaagcaa aattataact tggagccctg agtgcctagg
     3721 caaaaggtaa aattgaggcc gtaaaagata catttttcga cgcaaaatac atacttttga
     3781 aaacaaatca aagtttatgc ttaaaacgat tttattactg actcccgtgt atgtaacctt
     3841 gttttggact ttagttctga aaacggcaaa cagacacaat aacgatcgag ccaggctgtt
     3901 tctgggcaaa tttatgtttt ttgcttgtat cctgtatttt tcccattttc tttttttcta
     3961 tcctctacct caggtttata tttatttcga cgcactttac caatatgcct ctttgatggt
     4021 ataccccctt ttttacatct acattcgcct attgacagtg gataaaaaat ttgaacttaa
     4081 aaagcattac aagtacatcg ccgtacccaa tctgcttttg atcgcctatt tgataggcat
     4141 actgtttagc ccttatgaac actatcgagc actgatcgcc gactatgaaa acagtccctg
     4201 gaatttcctg aaaatagttt tgatctgtat gcagcttacc tttttaattc aggtaattct
     4261 ttgtgttaca ggcaacacca tcttattgaa aaaatacaaa cacagcgccg gacaatacta
     4321 ttccgatata gaggacagca gaatctacaa ggccgaattg ctcaatctgt gcattattct
     4381 tacaggtatc tcctctttta tactcggaat actgggacgt gatttctttt ttgccgagcc
     4441 tgtagctatg ggcattgctt ccttggtttt ttccaccttg ctttttgcta ttggtttact
     4501 cggcaatgtg cagaaagtag tcaatccaag ttatgaaacc gacagcggcg tccaaccggc
     4561 agcgaacttt gcctccgata aggaaatgga acacttcaaa gccgaagttt ctcctaaaat
     4621 tctgcgcctg tttaatgaag ataaaatcta tctgaacagc aaactcacca tcagagatgt
     4681 ggctcaaata gtcggaacga accgtactta cgtatccaac tttattaaca ctcattttaa
     4741 tcaaaacttt tgcagttttg taaatcgtta cagactcgaa gagctagaac gaaccatccg
     4801 taaacacccg gaatacaccg gcaacaccct ggccgaactc tgtggtttcg gttcaacgga
     4861 ctccctgaaa agggctgtaa ggactagttt tggtatgtct tattcagaat ggagaaaaag
     4921 tttgtctgtc caagcagaca aacatcaaga caatttgaac gccaaggcgt aattgcgaat
     4981 ttgcttgagc atcgccgcaa aaccattgga tctggtgggc gacaaatgct ccttgagtcc
     5041 aatgcgttca ataaaatata agtcggtctt cagtatttcg tccggtgtcc ggcccgaaag
     5101 aaccttcagc aacaggtaaa ccaggccttt tacaataacg gtatcgctct cggcctcaaa
     5161 aaacaccttg ccctccttgt aatcggcgtg tatccacacc cggctttggc atccttctat
     5221 cagatattga ggttttttgt actgttcatc cagcggactt aaagaattgc ccatgtcgat
     5281 taataattgg tacttgtcca tccaatcttc aatctcatcg aaggcctgaa tgatgtcgtc
     5341 ctgaacttcg tctatactca tttcaatctg ttaattaaac attttgtaga ttttctttaa
     5401 agcagcctgt aaacgatcta cttctgagct tgtattgtaa aaagcaaaag aggcgcgaac
     5461 acagctatct actgccaaac gctccatcag cggttgagca caatggtgcc cggtgcgtac
     5521 ggcaatgccg agtttatcga gcaacaagcc aacatcatac gcatgaatgc cttttaattg
     5581 aaaaggaatt atggcatgcc tctcaggtat atttcccaac aactgaaaac cttccacttt
     5641 gctcagctcc tgcaaggcat agttcaccaa ctgcttttcg tagtctgcaa tcttatccag
     5701 gcctatctcc tgaataaaac gtaatgcctc ggccaaggcc gctgttccnn nnnnnnnnta
     5761 agtccaacgg cgccctctac attttgagta ccagcctcga atttaaatgg aatccgattg
     5821 tatgttgttt tttcaaaatg aacttggtca atcatttctc ctcccccttg gtaaggaggc
     5881 atttgctcca aaaaagagcg tttcgcgtat aaaactccta ctccggtggg cccaaaaacc
     5941 ttgtgggacg aaaaggtata aaagtcggcg tccagagctc gaacatctac cggcatatgg
     6001 gcaactgcct gagctccgtc aaccagcaca ggaacacctt gcttgtgggc tttttcaata
     6061 atctgcttta tgggattctc gattcccagt acattggaaa catgagtaat ggccagtaat
     6121 tttaccttgg gtcccaataa tttttcgtat tcatcgacca acaaacaacc gttatcgtca
     6181 atgggaatca ctttaagagt cattttttta cgctcacaca agagttgcca gggaacaatg
     6241 ttagagtggt gctccatgct tgaaatcaaa atttcatcac cctcccggca aaaagcttcg
     6301 ccaaaggaaa aggccaccag gttgatagat tcggtggttc ccctggtgaa aacaatttca
     6361 tcgggttctg ccgcattgat aaattgctgc accacaactc tggccgcctc gtgagcttcg
     6421 gtagcctgtt ggctcaataa atgcaccgcc cggtgaacat tcgcattatg gctgaagtag
     6481 gcctcttcta tcttttttac cacggaacgc ggtttttggg tagtggctgc gttatccaga
     6541 tagaccaaag gatgcccgta ggctttttgt tccagaatag gaaagtgctt tcttatggaa
     6601 gctacatcaa atttcgattc catgacctca gttttattct caacaaatat cgcagccggc
     6661 acaagcgctc atctgtttac gaaatcgctt gcccaccaga ttgtggattc tgttttgcaa
     6721 gggaattagt tgaatcccct caatcacgtc tgccacaaag gcaaatttaa gcatcgcccg
     6781 ggcttcctgc tccgaaagac ctctcgatcg catataaaac aaagcgttct cgtcaatttg
     6841 gccggtggcg gaaccatggc tgcacttgac atcatcggcg taaatctcga gttgaggcaa
     6901 agcattcatc ctggcccggg atgaattgca gagattggca ttggactgat aagcagaagt
     6961 tttttgcgcg ttgggatgca ccagcacttt tccggcaaac acccctctcg attcatcgtc
     7021 cagaatatat ttatacagtt gacggctggt gcattggggt actacatgct ccacatcaac
     7081 atgataatcg gcaaactgat gactatcgag cagacaaaga ccggacaaca tagactcagc
     7141 ctgttctcct aacattctca aatttaaatt gttgcgtgaa actccattgt tcaaggtaat
     7201 accgttaacg aataccgaag aagaggcctg ttggtcgata aaagtattgc ttagccttgc
     7261 attattagga tgtgtcattt ccaattcata gtattccagc cgggcctttt taccgacaaa
     7321 aacctcggtt acctgattcg aaaaacaaga gctctgagtc atgctgtgat cacatatcat
     7381 cagcttggcc gaagctccgt cttcgataat aaccaagttt cttcggttag ccatttgctc
     7441 gatttcaccg tgaaatagtt gaatcagttg caagggctgt tcaatttcaa cacccgcagg
     7501 cacaaacaaa agaaaaccat cctgggccat ggctgtgttt aatgcggcaa aagcatcggc
     7561 agattgtccg gctatacggt cgtaatatgg ggccatgagt tctggatact cacggagtat
     7621 ggcgttgatg ctgccataca agacgccttc ggataatttt tgcggatcgg gatcgggata
     7681 atatacgtcg ttgaccacaa aaaacaattt ggtcgataaa ctgggaactt cgcaacgaaa
     7741 aaccttactg ggatgggccg gagccgacaa gcgatagagg ttcattccgt aatcaatttc
     7801 caacaaagca tccagccggc attgccggta agcttcgtct ttggggcccg gaaagccggc
     7861 cttttcaaaa agcccaatgg cagcctcccg ttttgagtcc atcaaaggag cagaatactg
     7921 cctgagcact tcccggtagt tcctgaacag attcaggtag gttgtttctt ttcccatttt
     7981 aatccttcaa ttcttgtttg acccagtcgt atccttgttt ttccaagtct ttggccaatt
     8041 gaaaatcccc gcttttgact atacgtccct cataaagcac atgcacccgg tcgggaacaa
     8101 tatagttcaa caatcgttgg taatgggtaa tgactataca ggatttctga ggggtatgta
     8161 ttttgtttac tccctgagca acaatgcgta aggcatctat atccagaccg ctgtcggtct
     8221 cgtcgagaat agagagtacg ggatcaagca tggccatctg aaatatttcg ttgcgctttt
     8281 tttctcctcc cgaaaaaccc tcgttgaccg aacggccggc caaggtatta tccaactcaa
     8341 ccaatgcctt tttttctcgc atcagtttca aaaagtccga ggcgttcatg gcttcctgtt
     8401 tgcggtattt acggtgctca ttaatggctg tgcgcataaa attcaccata cttaccccgg
     8461 ggatttccac cggatactga aaactcaaaa acaaaccttc gcgtgaacga tcttcgggag
     8521 tcatacttaa aagatccatg cctttgaagc gaaccgtacc ttgggtaaca gtaaaggccg
     8581 gatttcccga aagtaccgct gaaagcgtac ttttccccga gccattgggt cccataatgg
     8641 catgtatttc tccttctttc accgataaat tcaggccttt caggatttct ttatctcctg
     8701 cggaagcatg taaatttttt atttccaaca acataaatat cgtctttacg gccttggcca
     8761 atctgttttt attctttgtt tgttttctat ctcctcccgg tcttttttcc acgttttacg
     8821 gacttcggcg caggagaata ccttgatggc tttctttttg ctttaccctt tttcctcgtc
     8881 gtttttttga accagccctg aacaaacacc aataatccta caaccagcaa catcaaggca
     8941 agggcactca ccaaaagccg ctgcctgttt ccctcttctt ccaaaggcag ggtactaaac
     9001 aacaaataac ccaagcctgc caggatcaag agaataaaga tggcgaccca aatggctcct
     9061 ctgctctttt gaataggagc ttcgttgtcg tcagttcctc tattttgtgc ggtttttgtt
     9121 tccctttttg atttcatcct gctgtttata ttgtttattc caatcaacta ttgctcttaa
     9181 cgctatcaaa gtaaagacgg caaaaagcag cgctgtaaaa aacaacccca tataagcata
     9241 aagggctgtg ctcaacacgt cgacaactat ccagataatc cagttatcct ttacttttct
     9301 tgaaagcaaa aactgcccca ttacactagc cgctgtcaac atggcatcca acctgggaaa
     9361 ggctggagga tcaatgtatt cgggaaacct ggactgaaaa taaataattc cttcgctcca
     9421 caagccgccg aacaccaaaa ctatcactat caccagagcc cactggctct tatttaatcg
     9481 ccgtatgcgc agctctctgt ttttcccttc ttgtccgggc gccgggtgct tccagtgata
     9541 atacccatag aggctgaatg agaaatacac tacctgcagc agcatggctg agtataaatg
     9601 tgcctgataa aacacaacaa agtacaccac attgttgagc aaaccaaaca aaaaattaac
     9661 agagagcgct cttgaggcca aatatacagc caccagcccg ctaaccaatc cgatcaattc
     9721 cagcaggttc atggaatggc cccataacga aaacaacact acatcctgaa tcgacatatt
     9781 atccaacact cccttccaga gaaacctgca acaatttttg tgcctcaacg gcaaattcca
     9841 tgggaagctt attgattact tctttagcat agccattcac tatcaaccct atagcttctt
     9901 ctgtagaaat accgcgctga ttgcaataaa acaattgatc ctcgttgatc ttagaagtag
     9961 tcgcttcgtg ctcaactacg gctgtttcgt tgtttatttc aatataaggg aaggtatgtg
    10021 ccccacaggt gtcccccagc aacaaactgt cgcattggga gtgattgcgg gcgttggaag
    10081 ctttaggagc aatttttacc aacccacggt aactgttttg gcttttaccg gccgatatac
    10141 ctttggaaac aataatgctg cgggtgtttt tgcccaaatg aatcattttg gttcccgtat
    10201 cggcctgttg atgattgttg gtaacggcaa ccgagtaaaa ttctcccaca gaattatccc
    10261 ccgccaaaat acaggaaggg tacttccagg ttatggctga gcccgtttca acctgagtcc
    10321 aggagatctt ggccgattca cctttacaca aacaacgctt ggtaacaaag ttataaatac
    10381 cacccttgcc ttctttatcg cccggatacc agttttgtac agtagaatat ttgacttctg
    10441 cccggtcgtg gacaacaatc tccactatag ccacatgcaa ttggttttcg tcgcgtatcg
    10501 gagccgtgca gccttccagg tagctgacgt agctatcgtc atcggccact atcaatgtgc
    10561 gctcaaattg acccgtattg atggcattaa ttctgaaata ggttgacaac tccataggac
    10621 aacgcacccc cttaggtatg tacacaaagg aaccatcgct gaaaacggca caattcaggg
    10681 ccgcgtaata attatccgta tagggtacga ccgagcccag gtaacgcttg attaattcgg
    10741 gatgctcttg caccccctcg ctgaaagagc aaaaaatgat gcccttcttt gccaggcttt
    10801 ccttgaaggt ggtcttgacg gaaacactat ccattacggc atctacggcc atgcctgcca
    10861 gttgcttgcg ctcttgcaag ggtatgccca gtttctcgaa ggtttttagc aaatccggat
    10921 caatgtcttc ctcgttatct ccggaagttt tgctcttagg ggcggcatag tagaccattt
    10981 tttgatagtc aatttcggga atgtccaaat gcgcccattg gggcttttcc atgctttgcc
    11041 aatgacgaaa agccttgagg cgaaactcca acagccagtc cggctccatt tttttggaag
    11101 aaatcaaacg aataacatct tcatttaacc cctcgggaat agtttcggtt tcaatatcgg
    11161 tcacgaaacc atacttgtat tcaccggcag ttacctgtgt gatgatatct ttgtcttcgg
    11221 ttgatttcat acatcatata ctctgaggga caggtgttga ttgttgtaaa gtttcgggtt
    11281 tttttgtgtt ccagtcttca aagtggataa agggcagtac ggcgggagca aaatcggaaa
    11341 gcgggccgta aaaacgagaa gtactgcttt tttcccgatg aaaagaagac ttccctttgt
    11401 caaaatagtc aaacacatta acgactatac tcaacacaaa aaggtaaagc accacggcaa
    11461 ataaacctcc ggcaatgcgg ttgagccagc cgagcatcac cattttgacc agcttgccca
    11521 gtaaaaccgc tacaaaatga aacaagatga ccgtcagcac aaaaagtact ataaacgaaa
    11581 caacctgcac cacttttagg ctcgccgact caaaaagttg ggaaatgtaa ggagccacca
    11641 aatacgagag tgctttggct gcaaagattc cacaaacaac gcctgctata gttccaatgg
    11701 tctgtatcag acctctgcgc aaaccatcaa ttaagcctaa caaagcaacg atcaaaataa
    11761 tgatgtcgta gagattcata cgctacaggg tctgttttac ttctatttct tcgtaggctt
    11821 cgattaaatc gcccaccttg atgtcgttat aattgttaat actcaatcca cactcataac
    11881 ccgaggcaac ttccttgaca tcctccttga agcgctttaa tgagctcaat tctccggtaa
    11941 atacaactat accgtcacga ataagcctga ttttagtgcc tcgcttgatt ttcccttctt
    12001 taaccataca gccggcaact gtaccgatct tagtaatctt aaatacttca cgaacctcgg
    12061 ctgtggcagt aacttcctcc ctgatttcgg gagaaagcat accttccata gccgacttaa
    12121 tttcttcgat ggcatcgtag ataattgaat aaagacgaat atctacgcca tcgcgctcgg
    12181 ccatcttgcg ggcggccata gaggggcgta cctggaaacc gacaataata gcatcggaag
    12241 cagaagccaa cattacatcg gtttcagtaa tagctccaac tcctttatgt ataatgttaa
    12301 tttgaatttc ttcggtagat aattttgaga atgaatcaga taaagcttca acagaaccat
    12361 ccacatcacc tttaagaata atattcaatt ctttgaactg tcctaacgca attcttcgtc
    12421 caatttcatc taatgtaata
//
LOCUS       DAOSGL010000025        12217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4380_length_12217_cov_4.356520, whole genome shotgun sequence.
ACCESSION   DAOSGL010000025 DAOSGL010000000
VERSION     DAOSGL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12217
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_12217_cov_4.356520"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PKY68_01720"
     CDS             complement(<1..932)
                     /locus_tag="PKY68_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOH23789.1"
                     /translation="MNWVDELLQVKTVKALPIMTHPGIEKIGKTVRDAVTDGRVHYEA
                     IRYLSDHYPSMACTVIMDLTVEAEAFGSKITMPEDEVPSVTDRLVQDREGIEALEIPG
                     MDAGRLPEYLLANRLAVENIKDKIVLSGCIGPFSLAGRLFGLSEIMMEIYMDPDTIHL
                     LLDKCTRFLISYCKALKTIGTAGVFMAEPAAGLLSNEDCQTFSSDYVRQIVEALQDDR
                     FSIILHNCGNSGQCTQAMIDSGARVLHFGNLTDMEEALRVCPPDVLVMGNLDPVGLFK
                     LGSPQKVRESSRQLLDKAKSYRNFVISSGCDIPP"
     gene            complement(944..2503)
                     /locus_tag="PKY68_01725"
     CDS             complement(944..2503)
                     /locus_tag="PKY68_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478662.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOH23790.1"
                     /translation="MRTLVELSTLDIIIFVAYLVILGLVAAWASFGNKKKEGNLFLAN
                     KSLGWFAIGLTMWGTNVGPSMLIANASSGFEGGIAAGNFSWYAFPFIMLLAFVFAPRY
                     LGAKVTTLPEYMGKRFGEQTRNYIAWYSILTILISWLGLTLFSGGVFMAQLLAIPLWA
                     CIAILVAVSALFTMAGGLKAIAYTNVYQMLLLIGVSVLLVVMGINKVGGLGSVVRDTP
                     VEFWNLFRPLDDKDFPWLAILVGYPVMGVWFWCTDQSMVQSVLGAKNLKEGQKGANFC
                     AWLKLLDIPLFILPGILCFLLLPGLANSTDSYLQLVNAVFPSGLMGLVIVVMVAALIS
                     TIGSALNSLSTVFTMDIYIKKFRPDAGDSNIRTIGRRVVFVGALISVFLAIGITYIQG
                     LSFFNIFQSVLGFLAPPMAASFLFAAFWKKTSTQAINLVLTVGTAFSIGTGILYYTGL
                     IFSGMHYLYLSFFIFVVLALFIFIWSKAVSKPSLQKMEYVPIKVNKKVKIAWGILVLC
                     MIGVYLFFNGF"
     gene            complement(2506..3570)
                     /locus_tag="PKY68_01730"
     CDS             complement(2506..3570)
                     /locus_tag="PKY68_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23791.1"
                     /translation="MHNWKSNWETSKKHYMDWWQHQGIVLTMWEHIDKEGAPWEQVPA
                     PAPAKDLNQFWFDPEWRSGYLHHKMAGYSFMADILPVANTQLGPGSLGAILGAELEGR
                     EDTIWIRDQAGFDGNIVLDPNNRWWQLHRDLLKKCRQKSQDKYFVGMPDLVEGLDVLS
                     SLKGPDNVLMDLILDSEGSLQQLKAINTAWFEVFDRLYDIIHVNGEMAFCYFSIWGPG
                     KVAKLQCDISVMISEEDFRTFSLPFLKEQCEYLDYSLYHLDGVDAIRHLDAVLELKQL
                     NAVQWTPGVGQPQGGDPRWYDLYKKILDGGKSVMINWVQLNELEALLDTLGNRGLNIQ
                     MDFKSEKDIEQALNIVEKYR"
     gene            complement(3581..5791)
                     /locus_tag="PKY68_01735"
     CDS             complement(3581..5791)
                     /locus_tag="PKY68_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOH23792.1"
                     /translation="MRPSNYQLFDFLDFNMELNEGERLWRACRPLDIFVEGSDVLVQV
                     PFQQQENSNEIRPDIKVPQKILTLRLRAYGSAILRLTTAAGSAFREDSAMLERDPGLK
                     VEPLSVEKQPGQWTVYDTQKLVRAVVSFAEPEIDWWSDLQPAPDETIDLRFYPDGEKE
                     IRLSSYDMFSPARHDAFALAYVEQDNAVRRMTCAFHAEPDECFFGTGERFAKMDLSGR
                     TLVLYNQDGQGVNNRRTYKNVPFYLSSEGYGLFLHTSAYAKFSLADHSTRSAQILVDE
                     DCLDVFLIASSQPERILYNYRCLTGFPQMPPLWSFGTWMSRMTYYSEEEVKGICERLR
                     SEEYPCDVIHLDTGWFETDWLCEWKFNIERFPNPPAFIRQLKEKGFRVSLWQLPYIAY
                     NAAQYEEARENNYICKTKKKIQGASNFSVQDYAGTIDFTYDKATEWYKSLLRPLLEMG
                     VVCIKTDFGEDIHMDAEYHSMSPEKLRNLYPLFYQKAAWEVTAEVNKEALIWARAGWA
                     GCQRYPVHWGGDSACSWDGLAGSLKGGLHLGLSGFGFWSHDVPGFHGVPNFMNSVLPE
                     DIYVRWTQFGVFSSHLRYHGSHKREPWHYPRIAPIIKKWLNLRYSLIPYILEQSLACT
                     RSGFPLLRALYLHHPQDKICRHIDDQYYFGDNMLVAPVMNSEGIRDVYLPEGEWVHFF
                     SRKKYSGGRWIKAMEVPLDEMPVFVKAGSSIPLYPEKVSCTDEMDMDKVIREQF"
     gene            6081..9149
                     /locus_tag="PKY68_01740"
     CDS             6081..9149
                     /locus_tag="PKY68_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOH23793.1"
                     /translation="MRFIRPFPLPLKPLLLLSFCFLWFFSPEQKLKAQNTEEGLLLHY
                     SFDMIENTSILDESGNTNHGSIFGSAFSTGGWSGNALEFREVGDYALLPNNINASLND
                     FSIAVWVKGNASWNTWCRIFDFGRGTDYYMFLTPLAGSGTARFAFKNGGGEQIVDASG
                     PLPYNEWVHVCLTVDYTGDTGLGILYINGIESGRNENIGITPAMLSLEAETNANYLAK
                     SQFSDPGLNGALDDFRIYNRVLSVEEIALLSGFPIELLQQHQALDLGDLSQVSSNLNL
                     PLSLGDRGVQVDWTSSHPDIISNQGVVQRPEYFNASVTLTANLSLTEGGKSYSLTKVF
                     HAIVLALRIPEELLARWNFNNDNLLLGGEQIQVLDASANQFIATCMNRAKIITIGENP
                     RFNVLNLGHSNGYFDMGTEIGAAIYALEDYSIGIFFRVDESYGNLSAYGNWVWNFSNS
                     DQLATEANGALWGALRSQQQTICRTHYGTEQTVQLGSPAPQGKWHHMAATYNKETQTA
                     ILYIDGIAVATNNRVSQTCANALAISGRSGTLYNWLGRSCYTGDAYLAQTLLYDFQLH
                     RVTLSQTELNNHFSITNTLMALNAAYEENPDYHNPLLPQEFEALSLVDTLDQDLTLPV
                     QGRIDPGISIKWSSSHPEILSNEGVVNRPDYVDFIVSLTATLQSGADYMNKTFRVVVK
                     ANEGSSFKGNLILHHDFSVRVDSLVKDRAEKQFDAVLKEGAQVRKIGLNDNSFKVLDL
                     GKSQTAYLDLGEEIGKIIPSLSDFSIACYYRIDKEYQDLNWNGNCLWNFSNSDDIYTE
                     PLGYMSAFLNNQGYYISSGNWISEQSLATGSTAGKDSWHHLTYTQQANTGTLYLDGLM
                     VASGVLSAKPLNSLQKKGLNGTSCNWIGRSPYAGDLYLQNTLVYDFRVYNKALSPFDI
                     SDGLLNVPQTLGALNLAYSQTSSLGNTRKQQDIDLYTGTGLLLLRGLEPGDHIRILSL
                     TGSDIKHYYSTGNSISINSSEFQTGMYLLQLIREQTCVWTTKVIIPNPAL"
     gene            9146..10381
                     /locus_tag="PKY68_01745"
     CDS             9146..10381
                     /locus_tag="PKY68_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOH23794.1"
                     /translation="MNKALLFLVLCALAGTAKAAVDYQVRISEFMASNKSTILDSEGK
                     SSDWIEIHNADSEAVMLEGWSLSDERKNPRKWVFPAVEIEAGAYLLVFASGKNRRDPG
                     EELHCNFKLSSAGEYLALYSPEGVAYSCFDTYPAQEADVSYGYIDSCLFFFEQATPGH
                     PNEQSIAIRLLSPVCSHPRGYYDTAFVLTLESLVPGADIYYTLDGSPPVPGHSTLYEQ
                     TLSVTGNTILRAVCAKENYLPSKAATLSYLFIDDILNQSNSPEGYPEFWGSYAQKTGT
                     AVADYEMDPELIAEPAYAASVREGLRDLPVVSIAGDKNHFFSHEKDSVNGGIYIYTGP
                     PVGKDKTGDGWERPVSFEYFNSADSVSLQVNCGIRIHGGHSRLAEKSPKHSFRLVFKP
                     KFGPTRLDFPFSDLRGLKN"
     gene            10393..11925
                     /locus_tag="PKY68_01750"
     CDS             10393..11925
                     /locus_tag="PKY68_01750"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOH23795.1"
                     /translation="MRAGFCNSWIHSDAEARTIAQYIRDAWAKNTQQQMGHPASCNSY
                     AHLFINGLYWGLYNPSERIEDDYCEIHFGGDKEDYDVIKQDEEQNNIIYASDGNTTAW
                     FKLISALKTIPGQAVYQEIQGKNPDGSPHASKEALLDMQNFIDYMLINFYGGNTDWDH
                     HNWIAVRNRTNSAQGFRIICWDSEHILKSPNENRLSLNKTNCPSFIFQQLRNCAQFRL
                     DFADRVQKYFFDGGLLSPDGAAATWTAIADIIDKAVYAESARWGDYRRDVHPSNPPGV
                     LYRKDVHYDAQKKYLLETYFPQRSGIFLNQLKAASLFPQVEAPEILLNDEKVSRDTLN
                     IHDVLCLKTSRGSVYYSLDGQDPLDWSGGQEAVLSASSQLYTGAFSPDSSIILKARTL
                     YNNNWSALSEKQFFLKKPVSALNEISSDPETPGLTCHPNPVHLDGVISYHLPYKADVK
                     LSLYALDGTKIREWQEQNREAGRQQVYLDASGLQPGFYLLRLDVLGMDASCSATVKLI
                     RK"
     gene            complement(11926..>12217)
                     /locus_tag="PKY68_01755"
     CDS             complement(11926..>12217)
                     /locus_tag="PKY68_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH23796.1"
                     /translation="LAEAAYTYRDTVTQLSGLSVPPEDIYFARGCLHYNLPLEGSIRV
                     EIFLPDGRKVLTSHEPNAVGHRSLDCSELRPGLYIYRMYYSGTVSSGKILIP"
BASE COUNT         3383 a         3090 c         2737 g         3007 t
ORIGIN      
        1 tgcggaggaa tgtcgcaacc cgacgaaatg acaaaattac ggtatgattt ggctttgtcg
       61 agcaattgcc ggctgctctc cctgaccttt tggggactgc ccagcttgaa caacccaaca
      121 ggatcgaggt tgcccatcac caaaacatcc ggggggcaaa ccctaagagc ctcttccata
      181 tcggtgagat ttccgaaatg caagaccctg gccccactgt ctatcatggc ttgggtgcat
      241 tgcccgctgt tgccgcaatt atgcagaata atactgaatc gatcgtcttg cagagcttct
      301 actatttgac gaacataatc gctcgaaaat gtctgacagt cttcattgga caataatccc
      361 gcagccggtt cggccataaa gaccccggca gttccaatgg ttttaagggc cttacaataa
      421 ctgatcaaaa aacgggtgca tttatcgagc aagagatgaa tcgtatcggg atccatgtag
      481 atttccatca tgatttcgga aagaccgaac agccggccgg ccaacgaaaa gggccctata
      541 catcccgaca aaacgatttt atccttgatg ttttcgacag ccagccggtt ggccaataaa
      601 tattcaggca gcctgccggc atccatgccg ggaatctcca gggcttcaat gccttcacga
      661 tcttgtacca gacggtcggt tacagaaggc acttcgtctt cgggcatggt tatttttgat
      721 ccgaaagctt cggcttcgac ggtcaaatcc ataatgaccg tgcaagccat agagggataa
      781 tggtccgata aatagcgaat ggcttcgtaa tgcactctgc catcggttac agcgtcccgc
      841 accgtcttac ctattttttc tatgcccgga tgggtcatta tcggcaaggc cttgacggtt
      901 ttcacctgca gcaattcatc tacccaattc ataatttcaa actttaaaag ccattgaaaa
      961 acaaatacac tccaatcata cacaaaacaa ggatgcccca ggctattttt actttcttat
     1021 ttactttaat gggcacatat tccattttct gtaaagaagg tttagataca gcctttgacc
     1081 agataaaaat aaaaagagcc agaaccacaa aaataaagaa ggaaagatat aaataatgca
     1141 ttccgctaaa tatcaagcct gtataataca atattcctgt tccgatactg aaagctgttc
     1201 cgactgtgag caccaggtta atggcctgag tcgaagtttt cttccagaag gccgcaaaaa
     1261 ggaaactggc agccatggga ggagccaaaa agcccaacac cgactggaaa atattgaaga
     1321 aactcaaacc ctgaatataa gtgatgccaa tagccaaaaa gactgaaatc agtgctccga
     1381 caaagaccac ccggcgtcct atggtgcgaa tattgctgtc gccggcgtcc ggacggaatt
     1441 tcttgatgta aatatccatg gtaaaaaccg tgcttaagga attcaatgcc gatccgatgg
     1501 tactaatcag agccgctacc ataaccacaa taaccagtcc catcaagccg gaagggaaaa
     1561 ctgcattaac caattgcaag taggaatcgg tagaattagc cagaccgggt aaaagcagga
     1621 aacacaaaat acccggtagg atgaacagag gtatatcgag caacttgagc caagcgcaaa
     1681 aattcgcccc tttttggcct tctttcaggt ttttggctcc taataccgat tgcaccatcg
     1741 attgatcggt acaccaaaac cagactccca taacaggata acctaccaat atggccagcc
     1801 agggaaaatc cttgtcgtcg agaggacgga aaagattcca aaattccacc ggagtatccc
     1861 tgaccaccga acccaaccca ccgactttat ttattcccat taccaccagc aacaccgaaa
     1921 ccccgatcag taaaagcatc tggtaaacat tggtataggc tatggccttc aaacccccgg
     1981 ccatggtaaa caaagcagat acagccacca gaatcgctat gcaggcccaa aggggaatag
     2041 ccaataattg agccataaaa accccgcccg aaaacagagt cagacccaac caggatatca
     2101 aaatagtcag gatagaatac caggcaatgt agttgcgtgt ttgttctcca aaacgtttgc
     2161 ccatgtattc ggggagagtg gtgacttttg ctcccaaata gcggggcgca aacacgaaag
     2221 cgaggagcat gataaagggg aaagcatacc aggaaaaatt tcctgccgca atgccgcctt
     2281 caaaaccgct gctcgcattg gcaatcaaca tcgatgggcc cacattggtg ccccacatgg
     2341 tcaaacctat ggcaaaccaa cccaaacttt tattggccag gaataaattt ccttcctttt
     2401 ttttgtttcc gaaactggcc caggctgcca ccaggcccaa tattaccaaa taagcaacaa
     2461 agattattat gtccagagtg gacaattcga caagggttct catttctatc ggtatttttc
     2521 aactatgttt aaggcttgtt ctatgtcttt ttccgattta aaatccatct gaatattcaa
     2581 tccccggttg ccgagagtgt cgagcaaagc ctccagttca tttagctgta cccaattgat
     2641 cataaccgat ttacctccat ccagaatttt tttgtataaa tcataccaac gcggatctcc
     2701 tccctgaggc tgacccacgc cgggtgtcca ttgtacggcg ttcagttgtt ttagttcgag
     2761 cactgcatcc aaatgacgga tggcatccac accgtccagg tggtacaaac tgtaatccag
     2821 gtactcacat tgttccttca gaaaaggcag ggagaaagtc ctgaaatctt cttcggaaat
     2881 catgaccgaa atatcgcatt gcaacttggc aactttgccg gggccccaga tggaaaaata
     2941 acaaaaggcc atttctccgt tcacatggat aatgtcgtat aaacggtcga acacctcaaa
     3001 ccaggctgta ttgatggcct tgagttgctg cagactgcct tcggagtcta atatcagatc
     3061 catcaacaca ttgtcgggcc ctttaaggct ggataaaaca tccaggcctt cgaccagatc
     3121 gggcataccc acgaaatact tgtcctgcga tttttgacgg cattttttca acagatcccg
     3181 gtgcaattgc caccagcgat tgtttggatc caaaacaata tttccgtcaa atccggcctg
     3241 atctcttatc cagatggtat cttcccggcc ttcgagctct gcacccaata tggctcccag
     3301 cgacccgggc cccaattgcg tgttggctac gggtaaaata tcggccataa acgaataacc
     3361 cgccattttg tggtgcaaat agccggagcg ccattcggga tcgaaccaaa attggttcag
     3421 gtctttggcg ggagcaggcg ccggcacctg ttcccagggt gctccttctt tgtcgatatg
     3481 ttcccacata gtcaacacta taccttggtg ttgccaccaa tccatatagt gttttttact
     3541 ggtttcccaa tttgatttcc agttgtgcat ggttttgtct ttagaattgt tccctgatta
     3601 ctttgtccat atccatttcg tccgtgcagg ataccttttc gggataaaga gggatactgc
     3661 tgccggcttt aacaaaaacc ggcatctcgt ccaaaggcac ttccatagcc ttgatccacc
     3721 ggcctccgct gtattttttg cgactgaaga aatgtaccca ttccccttcg ggcaaataca
     3781 cgtcgcgaat accttcggaa ttcataacgg gagccaccag catattgtcc ccaaaataat
     3841 attggtcgtc gatatgacgg cagattttat cctgaggatg atgcaagtac agggccctga
     3901 gcaaaggaaa accgctacgg gtacaggcaa gactttgctc cagaatataa ggaatcaggc
     3961 tgtaacgcag gttgagccat tttttgataa tgggcgcgat gcggggataa tgccagggtt
     4021 ctcttttgtg cgaaccgtgg taacgcaaat gcgaactaaa gactccgaat tgagtccagc
     4081 gcacgtaaat atcttcgggc aaaacggaat tcataaaatt gggaacacca tgaaatccgg
     4141 gtacgtcgtg actccaaaag ccgaagccgg aaagtccaag gtgcagaccg cctttgagtg
     4201 aaccggccaa tccgtcccag gaacaggcgg aatcgcctcc ccaatgaacg ggataacgtt
     4261 ggcagccggc ccaaccggct ctggcccaga ttaaagcttc tttgttgact tctgccgtga
     4321 cttcccaggc tgctttttgg taaaacaaag gatagagatt acgcagtttt tccgggctca
     4381 tactgtggta ctcggcatcc atatggatgt cttctccaaa atcggttttt atgcaaacca
     4441 ctcccatttc gagcagtggc ctcaaaagag atttatacca ttccgttgcc ttatcgtaag
     4501 taaaatcgat ggttccggcg taatcttgca cactgaagtt ggaagcgccc tgaattttct
     4561 ttttagtttt acagatgtaa ttgttttcgc gggcttcttc gtattgggcc gcattgtaag
     4621 caatataagg caactgccaa aggctgaccc taaaaccctt ttccttgagc tgacgtataa
     4681 aagcgggcgg gttgggaaaa cgttcgatat tgaatttcca ttcgcaaagc cagtcggttt
     4741 cgaaccagcc ggtatcgaga tgaatcacgt cgcaaggata ttcttcgctt cgcaggcgtt
     4801 cacaaatgcc ctttacctct tcttcggaat aataggtcat gcgactcatc caagtaccaa
     4861 aactccataa tggaggcatt tgaggaaaac ccgtgaggca acggtaatta taaagtatcc
     4921 tttcgggttg ggaagaggcg attaaaaaca catcgaggca gtcttcgtct accagaattt
     4981 gagccgaacg ggtggaatga tccgccaggg aaaatttggc ataagcactg gtgtgcaaaa
     5041 acaatccgta gccctctgaa gaaagataaa atggaacatt cttgtaagtc cggcggttgt
     5101 tgaccccctg tccgtcctgg ttgtacaaaa ccagggttct acccgaaaga tccattttgg
     5161 caaaacgttc gcccgtaccg aaaaaacatt catccggctc ggcatgaaag gcgcaggtca
     5221 ttctgcgcac agcgttatcc tgctcaacgt aagcaagggc aaaagcatcg tgtctggcag
     5281 gcgaaaacat atcgtacgac gacaggcgaa tttctttttc cccgtccgga taaaatctca
     5341 gatcaatggt ttcgtcgggg gccggttgca ggtcgctcca ccagtcaatt tcgggttcgg
     5401 caaaactgac aacggctctg acaagctttt gagtgtcgta tacagtccat tggccgggct
     5461 gtttttctac cgacaagggc tccactttca acccgggatc ccgttcgagc attgccgaat
     5521 cttctctaaa tgcagaaccg gcagccgtag tcagccgcaa aatagctgaa ccgtaggctc
     5581 taagcctcaa ggtgaggatt ttttggggaa ctttgatgtc cggacggatc tcgttggaat
     5641 tttcctgttg ctgaaaaggc acttgcacga ggacatcgct gccttctacg aagatatcca
     5701 aaggacggca agctctccac aggcgctctc cctcgttcag ttccatgttg aaatccagaa
     5761 aatcgaaaag ctgatagttg cttggtctca tattggaaag ggctgagtgt actttagatt
     5821 aaactgcaaa gaaaacaaaa taaaccagaa ctaatacctt ttaggggtca ggcgtttttt
     5881 gaattttaga atctttataa tctgtaatgt tcccttttga aatacttccg actgtactgt
     5941 gctcttatat aaattcaatt agtaaatgca actaaaggct gccctcatct gaaattttat
     6001 gcgtattttt gacaaatttc agctctaatc tgtaaatata cccgactgta ttcacaattt
     6061 gccctgttaa ccatcatacc atgcgcttta ttcgaccctt tcccttgcct ctaaaacctt
     6121 tactgctttt gtctttttgt tttctgtggt ttttttcccc agaacaaaaa ctgaaagccc
     6181 aaaacacgga ggaagggctt ttgttgcatt acagtttcga catgatcgaa aacacttcca
     6241 ttctcgatga atcgggtaac accaaccacg gaagtatttt cggctcagcg ttctccaccg
     6301 ggggttggtc gggaaatgcg cttgaattta gagaagtggg cgattatgcc ctgctgccta
     6361 ataacatcaa tgcaagcctc aacgatttca gcattgccgt ttgggttaaa gggaatgctt
     6421 cctggaacac ctggtgcagg atcttcgatt ttggccgcgg caccgattat tatatgtttt
     6481 taacgccttt ggcaggaagc ggaaccgccc gttttgcttt caaaaacgga ggaggagaac
     6541 agattgtcga tgcctccggc ccgctgccct acaacgaatg ggttcatgtt tgcctgacag
     6601 tggattatac cggcgatacc ggtcttggaa tactgtacat caacggaatc gagagcggac
     6661 gaaacgagaa tatcggcatc acaccggcta tgttaagcct cgaggccgaa accaacgcca
     6721 attacctggc taaatcgcaa ttcagtgatc ccggacttaa tggtgctttg gacgacttca
     6781 ggatatacaa ccgggtgttg agcgtcgaag aaattgccct gctcagcggg tttccaattg
     6841 agcttctaca acaacaccag gcgctggacc tgggcgacct gagccaggta agttcaaatc
     6901 tgaatttacc cctgagtttg ggcgaccggg gagtgcaagt cgactggact tcgagccatc
     6961 ccgacatcat ctcaaatcag ggcgtggttc aacgtccgga gtattttaat gccagcgtca
     7021 ctctcacggc caaccttagt ctgacggagg gaggcaaaag ctacagtctg accaaagtct
     7081 ttcatgccat cgttttagcc ctcagaatac cggaagaatt gctggcccgc tggaatttca
     7141 acaacgacaa cttattgttg ggaggagagc aaattcaggt cttggacgct tcggccaacc
     7201 aatttatcgc tacctgtatg aaccgggcaa aaatcattac cataggagaa aatccgcggt
     7261 ttaatgttct gaatctgggc cattccaatg ggtattttga tatgggaacc gaaattgggg
     7321 cagccatata cgcgctggaa gattacagta tcggtatctt tttcagagtg gacgaaagct
     7381 acggcaatct gagcgcttac ggcaactggg tatggaattt ctccaactcg gaccaactgg
     7441 ctaccgaggc taacggagcc ctgtggggag cactgagatc acaacagcaa accatctgca
     7501 gaacacatta cggcaccgaa caaaccgtac aattaggctc acctgctcca caaggcaaat
     7561 ggcaccatat ggccgccacc tacaacaaag agacgcaaac cgccatattg tacatcgacg
     7621 gaatcgctgt agccaccaac aaccgggtaa gccaaacttg tgccaatgcc cttgcaataa
     7681 gcggacgcag cggcacttta tacaactggc tgggacgctc ctgctatacc ggagatgcct
     7741 acctggccca aaccctgctc tacgattttc aactgcatag ggtaacttta tcccagactg
     7801 aactgaacaa tcacttcagt attacaaaca cccttatggc gctcaacgca gcttacgaag
     7861 aaaatcccga ttaccacaat ccccttttgc cccaggaatt cgaagccctg agcctggtag
     7921 atactctcga tcaagaccta accttacccg ttcagggccg gattgacccc ggtatcagca
     7981 ttaagtggag ttcgagccat cctgaaatcc tctccaacga aggcgtggtt aatcgccccg
     8041 attacgttga tttcatcgtc agcctaacgg ctactctcca gagtggagcc gattacatga
     8101 ataaaacatt tcgtgtggta gtcaaggcca atgaaggaag ctcttttaaa gggaacctta
     8161 tacttcacca cgatttttca gtaagggtcg acagcctggt aaaagaccgg gcagaaaaac
     8221 aatttgacgc cgttttaaaa gaaggagctc aagtaagaaa aatcggattg aacgacaaca
     8281 gcttcaaggt gcttgattta ggcaaaagcc aaacagctta tctggacctg ggcgaagaaa
     8341 tcggaaaaat catccccagc ctctccgatt tcagcattgc ctgctattac agaatagaca
     8401 aagagtatca ggatttaaat tggaacggca actgcctgtg gaatttctcc aactccgacg
     8461 atatatatac cgaacctctg ggatatatgt cggcctttct gaacaatcag ggttattata
     8521 tttcctccgg caattggata tcggagcaaa gcctcgcaac aggcagtacg gccggcaaag
     8581 actcctggca tcatttgacc tacacacaac aagcaaatac aggcacttta tacctcgatg
     8641 gcctgatggt cgcctcgggc gtcctcagcg caaagccttt gaacagcctg caaaaaaaag
     8701 gcttgaacgg cacttcctgc aattggatag gacgctctcc ctacgcaggc gatctctacc
     8761 tgcaaaacac cctggtttac gatttcagag tgtacaacaa agccttaagt cctttcgata
     8821 tttcagacgg cctgctgaat gtaccccaga cgctaggagc gctcaatctt gcttacagcc
     8881 aaacgagttc cctcggaaat acacgcaaac agcaggacat tgatctgtac acgggaacag
     8941 gtctcctcct gttgcgaggg ctagaaccgg gcgatcacat ccgtatttta tcgttgacag
     9001 gctcagatat taaacattat tattcaacag gtaacagcat tagtataaac agttcggagt
     9061 ttcaaaccgg tatgtattta ttacagctta ttcgcgagca aacctgtgtc tggacgacaa
     9121 aagtcatcat tcctaatcct gctttatgaa caaagcctta ctgttcctag tcttgtgcgc
     9181 cctagccggc actgcgaaag ctgccgtcga ttatcaggta agaatttcgg aatttatggc
     9241 ctccaataaa tcgaccatcc tcgattctga aggaaaatcc tccgactgga tagaaattca
     9301 caacgccgac agtgaggccg taatgctcga aggctggtct ctgagcgatg agcgaaaaaa
     9361 tccccgtaaa tgggtatttc ccgctgttga gatagaagcc ggagcctatt tgctggtctt
     9421 tgcttccggc aaaaatagaa gagatcccgg ggaagaattg cactgcaact ttaaattaag
     9481 cagcgcgggc gaatacctgg cattgtacag tcctgaggga gttgcctaca gttgtttcga
     9541 tacctatccg gcccaggaag ccgatgtttc ttacggttac atagacagtt gccttttttt
     9601 ctttgaacaa gccactcccg gacatcccaa tgaacaatcg attgccatac gtctgctttc
     9661 tccagtttgc agtcatccca gaggatatta cgatacggcc tttgtgctga ctttggaatc
     9721 ccttgtcccc ggagccgata tttattacac actggacggc agtccgcctg taccgggcca
     9781 tagtacactg tacgagcaga ccctatccgt caccggcaac accattctaa gggctgtttg
     9841 cgctaaagag aattacctcc ccagcaaggc cgccacactg agttatttgt tcatcgacga
     9901 tatcctgaat caatccaaca gcccggaagg ctatccggag ttttggggca gctatgctca
     9961 aaaaacaggt accgccgtcg ccgattacga aatggatcct gaattgatag ccgaacctgc
    10021 ctatgccgcc tcggtacgcg aagggctccg cgatttgccc gtcgtttcga tagcaggcga
    10081 taaaaatcat tttttcagcc acgaaaaaga ctctgtaaac ggaggcattt acatctatac
    10141 cgggccaccc gtgggcaagg acaaaaccgg agacggatgg gaaaggccgg tttcttttga
    10201 atatttcaac tccgccgata gcgtttctct gcaagttaac tgcggcattc gcatacacgg
    10261 gggacatagc cgtctggccg aaaaaagtcc taaacattct tttcgtttgg ttttcaagcc
    10321 caaatttggc ccgacccgtt tggattttcc tttttcggac ctaaggggcc tgaagaattg
    10381 aacgctttgg tattgagagc aggtttttgc aacagctgga ttcattccga tgccgaagcc
    10441 agaaccattg cgcaatacat ccgcgatgcc tgggccaaga acacccaaca gcaaatggga
    10501 catcctgcca gttgcaattc ctatgcacat ctctttatca acggcctgta ctggggcttg
    10561 tacaatcctt ccgaaagaat tgaagacgat tattgcgaaa tacatttcgg gggagacaaa
    10621 gaggactacg atgttataaa acaagatgag gaacaaaaca acatcattta cgcttccgac
    10681 gggaacacta cagcctggtt caagcttatc tccgccctta aaaccatccc cggacaagct
    10741 gtctatcagg aaattcaggg caaaaatccc gacggttctc ctcatgcttc caaagaagcc
    10801 ttgctcgata tgcaaaattt tatcgattac atgctgatca atttctacgg aggcaacacc
    10861 gactgggacc accataattg gatcgcagtg cgcaacagga ccaactccgc tcaaggtttc
    10921 cggattatat gctgggattc ggagcatata ctaaaaagtc ctaacgagaa caggctcagt
    10981 ttgaataaaa ccaattgtcc cagttttatt ttccaacaat taaggaactg tgctcaattc
    11041 cggttggatt ttgccgaccg ggtacaaaag tatttcttcg acggcggcct gcttagtccc
    11101 gacggcgcag ccgccacctg gacagccatt gccgacatca tcgacaaagc cgtttacgcc
    11161 gaatcggccc gctggggcga ttaccgcagg gacgtacacc cttcgaatcc tcccggagtc
    11221 ttgtatcgca aagacgtcca ctacgatgcc cagaaaaaat acttgttgga aacctatttt
    11281 ccccaacgca gcggcatctt tttaaaccaa ctcaaagcag cctccctttt tcctcaggtg
    11341 gaagctcccg aaattttgct caacgacgaa aaagtcagcc gggacacttt aaacatccac
    11401 gatgttttat gtctgaaaac cagccggggc tccgtttatt acagcctgga cggacaggat
    11461 ccgctcgact ggtccggcgg acaggaagct gttctgtcgg cctcttcgca attgtacacc
    11521 ggagccttca gtcccgattc aagtattatt ctcaaagcca gaaccttata caacaacaac
    11581 tggagcgctc tgagcgaaaa gcaatttttc ctgaaaaaac cggtatccgc cttaaatgag
    11641 atcagttcgg atcctgaaac tcccgggctg acctgtcatc cgaatcctgt gcatctggat
    11701 ggggtgataa gctaccactt accctataaa gccgacgtta agctgagttt gtacgctttg
    11761 gacggaacta aaatcaggga atggcaggaa caaaaccgtg aagcgggcag acagcaggtt
    11821 tacctggatg cctccggttt acaacccgga ttctatctgt tgcgcctcga tgtactcgga
    11881 atggatgcct cctgctccgc caccgtcaaa ttaatccgaa aataatcagg gaatcaatat
    11941 tttcccgcta cttaccgtgc cggaataata catccggtaa atgtagagac cgggacggag
    12001 ttcggaacaa tccaagctcc tgtgtcccac tgcattgggt tcatgcgaag tcagcacttt
    12061 tcggccgtcg ggcagaaaaa tttctacacg aatgctgccc tcgaggggca aattataatg
    12121 caggcaacct cgggcaaaat aaatatcttc gggcggaaca gaaagccccg acaattgggt
    12181 tacagtatct ctgtatgtat aggctgcttc ggccaga
//
LOCUS       DAOSGL010000026        12147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4415_length_12147_cov_4.347089, whole genome shotgun sequence.
ACCESSION   DAOSGL010000026 DAOSGL010000000
VERSION     DAOSGL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4415_length_12147_cov_4.347089"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /locus_tag="PKY68_01760"
     CDS             complement(<1..1890)
                     /locus_tag="PKY68_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOH23797.1"
                     /translation="MSKTEENKPVTALPENAYRELKEGELYHPVMSPGRKYPEATVWS
                     VSWGVLMAILFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSSLTKRKNALGENVLIQSI
                     GSSSGVIVAGAIFTLPALYILQAKYPDMSVNFIQVFLSSLLGGVLGILFLIPFRKYFV
                     SEMHGKYPFPEATATTQVLISGEQGANQAKPLVVSGLIGGLYDFVVATFGSWTEVFTS
                     RVSQFGTMMADKFKLVFSINTGAAVLGLGYIVGLKYATVICLGSALIWFVFVPLLPLF
                     GDTFLQFLNPAISDGVAGMSPEVIFSSYARHIGIGGIAMAGIIGIVKSWPVIKSALGL
                     AAREMKGQGAKTAEQATQARTSRDLPMKIILIGIVLALLAVFVFFFFGVMHLNLLHAI
                     VGILIVGIIAFLFTTVAANAIAIVGTNPVSGMTLMTLILASVIMVAVGLKGSTGIVSA
                     LIMGGVVCTALSMAGGFITDLKIGYWLGSTPAVQQSWKFLGTLVSALTVGGVLIILNK
                     TYGFVGENALVAPQANAMAAVIDPLMSGTAAPWWLYATGALISLILNFFGVPALAFAL
                     GMFIPFQLNIPLVVGGLINWFVSGRSKDSKLNTTRNEKGTLIASGFIAGGALMGVVSA
                     ALRFGG"
     gene            complement(1902..2669)
                     /locus_tag="PKY68_01765"
     CDS             complement(1902..2669)
                     /locus_tag="PKY68_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOH23798.1"
                     /translation="MKREFGLIGYPLTYSFSQVYFNEKFQSEGIEAVYENFPLKRIEE
                     FPSLIQSRPALAGLNVTIPYKQSVIPFLDELGPEAASIKAVNVIVPEIKEGEFRLIGR
                     NSDYIGFRDSILPEIERLKASCPQQLKALILGTGGASKAVYYALQQMGLETSFVSRDA
                     AKAEYTYATLNKELMDRFKIIVNTTPLGMAPDTDSCPDLPYRYLGRGHLLYDLVYNPA
                     LTRFLSLGQQAGARVMNGAGMLKIQAEAAWKMWNGQL"
     gene            complement(2666..4039)
                     /locus_tag="PKY68_01770"
     CDS             complement(2666..4039)
                     /locus_tag="PKY68_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH23799.1"
                     /translation="MKSREDDNIRALVKRYEQQRSNGLSQYFDVDEFDRIADYYLDRG
                     RTNKLSEVVSSALRIHPNSTVIHLKRAILYVETGEPMKALQLLDRLPEQEEAGFVRAA
                     AFLKLKRKEEGLALLQDMVIREQYDKDLACLEAVNVLNAENLYKEALDFVRLGLEHNP
                     ENTDLMSEGAFCAEQLNDMPASEHYYRMLLEVDVYSYENWFNLGQILFSQERFEEACK
                     AYDFALVSNPGFMPAVLQMGHACFQMEHYEDAAKFYLDYLLAAIEESLPSAEGNSIDD
                     LMVFVGEAFEKAEHYESAIEWYEHALTTNPGNLQAYTGMGICYMELKDYKQSLRYLEK
                     AIKMDDKQAEIWVYLAEVLVDMDKNEQAIVAYLHALSLDPSQAEVLFALGNLYYDQQK
                     FELALKYYSDAELLDATFPNIHLYKALAYARNGDMLRADNSLKLALLSDVHARELFNK
                     IIGEEQK"
     gene            complement(4064..5761)
                     /locus_tag="PKY68_01775"
     CDS             complement(4064..5761)
                     /locus_tag="PKY68_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOH23800.1"
                     /translation="MTVVDTNSEGSKKSLNFIEQIVENDLKEGKNQGRIATRFPPEPN
                     GYLHIGHAKAICLDFGIAQKYGGTCNLRFDDTNPTKEDVEYVDSIMEDIRWLGFSWDN
                     LFYASDYFGKLFQFAQELIKRGLAYVDDQSAEVIAQQKGTPTSPGVNSPYRNRSAEEN
                     LDLFLRMQAGEFDEGSRVLRAKIDMAHSNMHFRDPLMYRILKQAHHRTGNTWNIYPLY
                     DFAHGQCDYFEGITHSLCTLEFEIHRPLYDWFIDHLQEGDYRPRQIEFNRLNITYTIM
                     SKRKLLQLVEGGFVKGWDDPRMPTLCGLRRRGYTPESIRNFIDRIGYTKFDGVIDQSL
                     LEFAVREHLNKIAPRVSAVLNPVKLIITNYPEGKVEWNEAINNPEDESAGSRSIPFAR
                     ELYVERDDFMEEAPKKFYRLTPGQEVRLKNAYIVQCTGCKKDADGQVQEIYCTYDPLT
                     KEGGPESKRKVKATLHYVTVEHSLPAEVRLYDRLLTVENPAEDKERDFMDMLNPDSLK
                     VLKDCRVEASLQDARPLDHFQFLRMGYFNLDPDSRPGQLIFNRTVGLRDTWSKIAKK"
     gene            6104..7510
                     /gene="uxaC"
                     /locus_tag="PKY68_01780"
     CDS             6104..7510
                     /gene="uxaC"
                     /locus_tag="PKY68_01780"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOH23801.1"
                     /translation="MKAFMDENFMLDNEVSEQLFHQHAKNLPIIDYHCHLNPEYIAKD
                     HKFDNLGQIWLEGDHYKWRAMRTNGIDERYCTGKDTSDWEKFEKWAETLPYTMRNPLY
                     HWTHLELKTAFGVNKILKPETAREIFDECTAKLRTPEFSARGLMRKYNVETVCTTDDP
                     VDSLEHHIALKKEGFEIKVLPTWRPDKAMAVEDPKSFRAYVEKLAQAAETKISRYNDL
                     IAALRIRHDFFASVGCKLSDHGIEEFYAEDYTQAQIEAIFNKVYGGKELSQEEILQFK
                     SAMMVEFALMDAEKDWTQQFHYGAIRNNNSRLFAQLGPDTGFDSIGDFNVGKNMSKFL
                     NRLDKNNQLAKTIIYNLNPRDNELIATMLGNFQDGSVAGKIQFGSGWWFLDQKYGMIA
                     QMNSLSVLGLLSRFVGMLTDSRSFLSYPRHEYFRRIMCNLIGVDVVKGEIPYSEMDFL
                     GKMVEGVSYYNAKTFFKF"
     gene            7583..8626
                     /locus_tag="PKY68_01785"
     CDS             7583..8626
                     /locus_tag="PKY68_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOH23802.1"
                     /translation="MSKRVVTFGEIMLRLATPEYLRFSQTSSLTATFGGGEANVAVSL
                     ANYGIKVDFVSRLPLNDIATSCVMNLRKYGVGTDHLIYGGERLGIYFLETGAVARPSK
                     VIYDRAHSSIAEIQPGMIDWRKIFKGADWFHWTGITPAISQGAADACLEAIQIANEMG
                     VTVSTDLNFRKNLWKYGKSASEVMPKLVAGCDVILGNEEDAEKVFGIKPEGFEVAHTG
                     GEVNAAEFESVCKQLMAKFPRAKKVIITLRGSINANHNTWRGCLYSDGSLKISKAYDI
                     THIVDRVGGGDSFMGGLIYGLLTYEGDDQKALEFAVAASCLKHTIYGDFNMVSVAEVE
                     KLMGGDASGRVSR"
     gene            8638..9309
                     /locus_tag="PKY68_01790"
     CDS             8638..9309
                     /locus_tag="PKY68_01790"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOH23803.1"
                     /translation="MAKFTKQQVINTICTSGMVPVFYNKDIEVSKQVILACYRGGVRV
                     FEFTNRGDFAQEVFKELVKYTEKTCPEMIMGAGSVVDAGTASLYIQLGANFIVGPLFN
                     PEVAKVCNRRLIPYAPGCGSVTEIGYAQEAGCDICKIFPAGNVGGPSFVKNVMAPMPW
                     SKLMVTGGVTPEESNLKAWFDAGVTCVGMGSNLFPADLIKSENWDGITKLCAEAMAII
                     QKVKK"
     gene            9347..10813
                     /locus_tag="PKY68_01795"
     CDS             9347..10813
                     /locus_tag="PKY68_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH23804.1"
                     /translation="MSKAQTVTQKMSQHRWVIVALLFFATTINYLDRQVLSLTWDEFI
                     KPEFHWDESHYGTITSFFSIFYAISMLFAGRFVEWMGTKKGYLWSIGVWSVGACLHAV
                     CGLVTEAHVGLHSAAELAAATGDVAVVIATFSMYSFLVARGILALGEAGNFPAAIKAT
                     AEYFPKKDRAYATSIFNAGASIGALFAPLTIPPLAKAFGWEMAFIIIGALGFIWMGFW
                     VFLYDAPRKSKFVNSQELEYIEQDKHEEIAEQKKKAAEEEKVPFLKILSFKQTWAFAL
                     GKFMTDGVWWFFLFWTPSYLYTQFDIKMSEGLGMALIFTLYAIVTVLSILGGKLPTVF
                     INRGGQNPYAARMKAMLIFAFFPLVVLLAQPLGMKFAGLGQHAAWIPVLLISIGCSAH
                     QAWSANLFSTVGDMFPHGAIATVTGIGGMAGGIGSMILQKSAGNLFVYAGETNMTFFG
                     FVGKPAGYFVIFCICAVAYLIGWIIMKSLVPRYQKIVI"
     gene            complement(11063..>12147)
                     /locus_tag="PKY68_01800"
     CDS             complement(11063..>12147)
                     /locus_tag="PKY68_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOH23805.1"
                     /translation="EAILRGTPHFEDYPVMQVRWEGKWIQGRTALIYAVDASYTGIVG
                     GGKIIGHPALGGRPTSDNPLRHPALIEPINCKNLLFSDFSTSYQRMWCIHPTYCENIH
                     FKDLTIRSTGGNGDGIDIDSCKHVLIERCDIETGDDCIAIKSGRGMEANMLMRTTEDV
                     YIRNCRLSDSIYACIGIGSETSGGIRGVRIENCRFENTRTHAIYIKSRPGRGAFIEDI
                     SAEGLDVSGMQGGFLRFNLMGSGLQDQVPVPGLQGIPTVKNFSFKNVRVRDVKDLVDG
                     TSVHPDKPLEGLVLENISGEALWGIRLANVRKAVLRNINVNIREGDALSIYNVQGKGL
                     GKAIEMGFPHKIEAAIAEPDPPYVLK"
BASE COUNT         3410 a         2825 c         2629 g         3283 t
ORIGIN      
        1 acctccaaaa cgcaaagccg cactgacaac tcccattaag gcgccacctg caataaagcc
       61 ggaagctatc aatgttcctt tttcgtttct ggtcgtattc agtttgctgt ctttactacg
      121 ccctgaaaca aaccaattga ttaaaccgcc cacgaccaga ggaatattca gttgaaaagg
      181 aataaacatt cccagagcaa aagccagggc aggtacgccg aaaaaattca agatcagtga
      241 aatcaaggca cctgtagcgt acagccacca aggtgcggcc gttccagaca ttaaaggatc
      301 tataacggca gccattgcgt tggcctgcgg agctaccaag gcgttttcgc ccacaaagcc
      361 ataggtcttg ttcagaatga taagcacccc tcccactgtt aatgccgata ccaaggtgcc
      421 cagaaatttc cagctttgct gaacggcggg ggtcgatccc agccaataac cgattttgag
      481 atcggtaata aaacctccgg ccatagacaa ggctgtacac actactccac ccattatcaa
      541 ggctgaaact atacccgtag aacctttaag cccaacggct accataatga ccgaggccag
      601 aatgagggtc atcaatgtca taccggagac aggattggtt ccgactatgg caatagcgtt
      661 ggcggctact gtagtaaaca gaaaagcaat tatgcccact atcagaattc ctactatggc
      721 gtgcaagaga tttaagtgca tcacaccgaa aaagaaaaag acaaataccg ccagaagggc
      781 cagaacgata ccgatcaata tgatcttcat cggtaaatca cgagacgttc tggcttgagt
      841 agcctgctca gctgttttgg ctccctgacc tttcatctca cgggctgcca gtccaagggc
      901 actttttata accggccaag atttcactat acctattatt ccagccatag caatgccgcc
      961 aatacctata tgtctggcgt agctgctaaa aataacttcg ggcgacatac ctgctacacc
     1021 atcgctgatg gccggattga gaaactgcaa aaaagtatcc ccaaacagag gcaacaaagg
     1081 aacaaaaaca aaccaaatca aagccgaacc caggcaaatg acagtggcgt atttcaagcc
     1141 gacaatataa cccagtccca atacggcagc gccggtattt atgctgaaaa ccaatttaaa
     1201 cttatcagcc atcatcgtac caaactgact taccctggaa gtgaaaactt ccgtccagga
     1261 gccaaaagtg gctacgacaa aatcgtagag tccgccaatt aatccggata cgacaagagg
     1321 ctttgcctga ttggctccct gctcaccgga tataagcacc tgagtggttg ccgttgcttc
     1381 ggggaaaggg tatttgccgt gcatttcgga cacaaaatac ttgcgaaacg gaatcaaaaa
     1441 caaaataccc aaaacacctc ccagcagcga actcaaaaag acctgaatga aattaacact
     1501 catatcaggg tatttggctt gcaaaatata cagagccggc aaagtaaaaa tggcaccggc
     1561 cactatcact ccggaagagg agcctatgga ctgtatcaat acgttctcgc ccaaggcatt
     1621 ttttcgtttg gtcaggctgg acaaaccaac agcgataatg gcgattggta tggccgcttc
     1681 gaaaacctgg cctattttca atcccaggta ggcagcagca gccgaaaaca aaatggccat
     1741 caaaacgccc caacttaccg accatacagt ggcttcggga tattttctgc cgggagacat
     1801 cacaggatga tacaactcac cctctttcaa ctctctgtag gcattttccg gcaaagcagt
     1861 taccggcttg ttttcttctg ttttactcat atcttagaat tttaaagctg tccgttccac
     1921 atcttccagg ctgcttcggc ctgaattttc aacatgcctg ccccgttcat tacacgggct
     1981 ccggcctgtt gacccaggct gagaaaacgg gtgagagcag ggttgtatac caaatcgtag
     2041 agcaaatgtc cccgtcccag gtagcgataa ggcaaatcgg gacaagaatc cgtatcggga
     2101 gccataccta acggagtggt gttgacaata atcttaaaac gatccattag ttccttgttc
     2161 aacgtggcgt aagtatattc agccttagcg gcgtcacgcg atacgaaaga agtttctaaa
     2221 cccatttgct gcaatgcata ataaacagct ttggaagcgc cgccggtgcc caatatcaaa
     2281 gccttgagtt gttgagggca cgatgctttt aaacgctcaa tctcgggcag aatgctatct
     2341 ctgaatccta tataatcgct gttgcggcca attaaccgga attctccctc tttgatttcg
     2401 ggtacaatca catttaccgc cttaatggag gccgcttcag gacccaattc gtccaaaaag
     2461 ggaataacgc tttgcttata agggatggtt acattcaaac ccgccaaggc cggccgggac
     2521 tgaatcaaag acggaaattc ttcgattctt ttcaagggaa aattttcata aacagcctca
     2581 attccttccg attgaaattt ttcgttaaag tagacctgtg aaaaagaata agtcaaaggg
     2641 tagccaatca ggccaaattc tcgtttcatt tctgttcctc cccaataatt ttattgaata
     2701 actctcttgc atgaacatca ctaagcaaag ccagttttaa agaattatcg gcccgtagca
     2761 tgtccccatt gcgagcataa gccagcgctt tgtataagtg tatgttggga aaagtagcat
     2821 ccagcaattc agcgtcggaa taatacttca gcgccaactc aaacttttgc tgatcgtaat
     2881 acagattacc aagagcaaaa agtacttcgg cttgcgaggg atcaaggctt aaagcatgca
     2941 aataggctac aatggcttgc tcattcttat ccatatcgac aagcacctcg gccagataaa
     3001 cccagatctc ggcttgttta tcgtccattt tgatggcttt ttccagatac cgaaggcttt
     3061 gcttgtaatc ttttaattcc atatagcaaa tccccattcc ggtatatgcc tgtaaattac
     3121 cgggattagt ggtcaatgca tgttcgtacc attcaatggc cgattcgtag tgttctgctt
     3181 tttcaaaagc ctcacctaca aacaccatta agtcgtctat gctattgcct tcggcagagg
     3241 gaaggctttc ttctatggct gccagcaggt aatcaaggta aaatttggct gcgtcttcgt
     3301 aatgttccat ttgaaaacag gcatgcccca tctgaagcac cgcaggcata aacccgggat
     3361 tgcttaccaa cgcaaaatcg taagctttgc atgcttcttc aaagcgctcc tggctaaaca
     3421 atatctgccc caggttgaac cagttttcgt aagaataaac gtccacctcg agcaacatcc
     3481 ggtaataatg ttccgaggcg ggcatatcgt tcaattgttc agcacaaaaa gcaccttccg
     3541 acattaaatc ggtgttttcg ggattgtgtt ccaagcctag ccgtacaaaa tccaaggctt
     3601 ccttatacag attttcggca ttcaatacat tcactgcttc aagacaagcc aagtctttat
     3661 cgtattgttc tctgattacc atatcttgga gcaaagccaa accttcttct ttccttttga
     3721 gttttaaaaa ggcggcagcc cgcacaaaac cagcctcttc ctgctcaggt aaacgatcaa
     3781 gcagctgcaa ggctttcatc ggttcgcccg tttccacata caaaatggca cgtttcagat
     3841 gaataaccgt actgttggga tgaatgcgca aagcagaact gaccacttcc gagagcttgt
     3901 tggtgcgacc ccggtccagg taataatcgg caatacgatc gaattcatct acgtcaaaat
     3961 actgcgacaa gccgttggag cgttgttgtt cataacgctt aactaatgca cgtatattat
     4021 cgtcctctct tgacttcatt cggattcaaa aaaatatatt gccttatttt ttagcgatct
     4081 tgctccaggt gtccctgaga cctacagtcc ggttaaaaat taattgaccg gggcgggaat
     4141 cggggtccag attgaaatag cccatacgca gaaattgaaa atgatccagc ggacgggcat
     4201 cttgcagaga agcctccaca cggcaatctt tcaaaacctt gagcgaatcg ggattcagca
     4261 tgtccatgaa atctctctct ttgtcttcgg ccggattttc taccgtcaac aaacggtcgt
     4321 ataaacgaac ttctgccggc aagctgtgct caacagtaac ataatgcagg gtggctttta
     4381 ccttacgctt gctttccgga cctccttctt tggtcagggg atcgtaggta caataaatct
     4441 cctgcacctg gccatcggca tcttttttac aacctgtaca ttgcacgata taagcatttt
     4501 tcaaccttac ttcttgtccc ggcgtcagtc gatagaactt tttaggtgct tcttccataa
     4561 agtcatccct ttcaacgtac aattcacggg caaagggaat tgagcgactg cctgcgcttt
     4621 catcttcggg attattgatg gcttcgttcc attccacttt cccttcgggg taattggtaa
     4681 taataagctt tacgggattc aatacggcac ttacccgggg agcaatcttg ttgagatgtt
     4741 cgcgtacggc aaattccaac aagctttgat ctatcactcc gtcaaatttc gtataaccta
     4801 tcctgtctat aaaatttcgt atggattcgg gcgtatagcc gcgcctgcgc aaaccgcaaa
     4861 gagtaggcat gcgtggatcg tcccaacctt tgacaaaacc cccttcgacc aattgcagca
     4921 attttcgctt actcattatg gtgtaggtta tgttcaggcg attgaattca atttgcctgg
     4981 gccggtaatc gccctcttgt aggtgatcta taaaccagtc gtagagcggc cggtgtatct
     5041 caaactccag agtacagagc gaatgagtaa taccttcaaa ataatcgcat tgtccgtgag
     5101 cgaaatcgta caagggataa atattccatg tattgcctgt gcggtggtgg gcttgtttta
     5161 agatacgata catcaacgga tcacgaaaat gcatattgga atgtgccatg tcaattttgg
     5221 ctctcaaaac cctgctgccc tcgtcaaatt cgccggcctg cattctcaaa aataaatcca
     5281 ggttttcttc ggcagatctg ttgcgataag gactattgac cccggggctg gtgggtgtgc
     5341 ccttttgctg ggctatcacc tcggcagact gatcatctac ataggccaaa cctcttttga
     5401 tcagttcctg tgcaaactga aaaagtttcc cgaaataatc ggaagcataa aacaaattat
     5461 cccagctaaa tcccaaccaa cgtatgtctt ccataatgga atcgacgtat tcaacgtctt
     5521 ctttggtggg attggtatcg tcaaaacgca aattgcaggt gcctccatat ttttgagcaa
     5581 tcccaaaatc cagacaaata gccttggcat ggccaatatg cagatagcca ttgggttcgg
     5641 gtgggaaacg ggtggcaatt cttccttgat tttttccttc ttttaaatcg ttttcgacga
     5701 tctgttctat gaaattcagg ctttttttgg agccttcact gttggtgtct actactgtca
     5761 tgttattaaa aaaatggttc caaagatact gcctaatttt gcaagattca aatgagcaac
     5821 aagaaactat gctccaaaaa catgttttat aatataagtt tttttttgcc tgtgtgcaaa
     5881 aaaagttcaa cctttgctgc cgttaaccta acacaatctt ttttaccttt gagactaata
     5941 cctattgaag actatatgaa gtccgccttg tgaaaggcgg acttcattct ttttagcctt
     6001 tccgctgcaa aagtaatgtt tgtttatcaa atatgaacct tcggctcatc aaaaaataat
     6061 ttgtatattc gcaaaatttt taaacctcaa aaatacatct actatgaaag cattcatgga
     6121 cgaaaatttt atgttggaca acgaagtatc cgaacagctt ttccaccaac acgccaaaaa
     6181 tctgccgatt attgattatc actgccacct gaaccccgaa tacattgcca aagatcacaa
     6241 attcgacaac ctgggacaga tctggctcga aggtgaccat tataaatggc gtgccatgcg
     6301 aaccaacgga attgatgaac gttattgcac cggtaaggat accagcgact gggaaaaatt
     6361 cgaaaaatgg gccgaaaccc ttccctatac catgcgtaat cccctgtatc actggactca
     6421 cctggaactc aaaactgcgt ttggtgtgaa taaaatactc aaaccggaaa cagccaggga
     6481 aatttttgac gaatgcaccg ccaaactgcg cactcccgaa ttcagtgccc gcggactgat
     6541 gagaaaatat aatgtcgaaa ccgtctgtac taccgacgat ccggtggata gtctcgagca
     6601 tcacatagct ctcaaaaaag agggttttga gattaaggtc ttacccacct ggcgtcccga
     6661 caaagccatg gcagtagaag atcccaaatc gttcagagct tacgtggaaa aactggccca
     6721 ggcagcagaa acgaaaattt cccgttacaa cgatttgatt gctgctctga gaatacgtca
     6781 cgatttcttt gcttcggtgg gttgtaagct cagcgaccac ggtatagagg aattttatgc
     6841 cgaagactat actcaggcac aaattgaagc catttttaat aaggtatatg gaggcaaaga
     6901 attgtctcag gaggaaattt tgcaattcaa atcggccatg atggtagaat ttgccctgat
     6961 ggatgccgaa aaagactgga cacaacaatt ccactacgga gctattcgta acaacaattc
     7021 gagattgttt gctcaattag gccccgatac aggttttgat tccatcggag acttcaatgt
     7081 cggtaagaat atgtcaaaat tcctgaaccg tctggacaaa aacaaccaat tggccaaaac
     7141 cattatatat aacctcaacc cacgtgataa cgaattaatt gccactatgc tgggtaattt
     7201 ccaggatggt tctgtagccg gcaagattca atttggatcg ggctggtggt tcctggatca
     7261 gaaatacggt atgatcgccc agatgaattc cttgtcggtg ctcggcttat tgagtcgttt
     7321 tgtcggtatg cttaccgatt ccaggagttt tctttcgtac ccgcgtcacg aatattttcg
     7381 tcgtatcatg tgcaacctta ttggtgttga tgtggtgaaa ggtgaaattc cttacagcga
     7441 aatggacttt ctcggtaaga tggttgaagg cgtatcgtac tacaacgcta aaacattttt
     7501 taaattttaa acgacattca taaacggctg caatgaaaat tttctttacc ttgcggccgt
     7561 ttattttaca acgacggatc aaatgagtaa aagagtagtt actttcgggg agattatgtt
     7621 gcgtttagct actcccgaat acttacgttt tagccagacc tcgagcctga ctgccacttt
     7681 tggcggaggc gaagccaacg tggccgtatc gctggccaat tacgggatca aagtagattt
     7741 tgtgagccgt ttgcccctaa acgatatagc aacctcttgc gttatgaact tacgaaaata
     7801 cggggttggc accgatcacc tgatttacgg aggcgagcgt ttgggtattt attttttgga
     7861 gaccggagct gtggcccgtc ccagcaaagt aatttacgac agggcgcatt cttctatcgc
     7921 tgagattcag cccggcatga tcgactggag aaaaattttc aaaggtgcag attggtttca
     7981 ctggacgggt attacgcctg ccatttctca aggagctgcc gatgcatgtt tagaagcgat
     8041 tcaaatagcc aacgagatgg gagtaactgt ttccacagat ttaaatttta gaaaaaattt
     8101 atggaaatac ggaaaatcag cgtcggaagt aatgcccaaa ttagtagccg gatgcgacgt
     8161 gattttgggt aatgaagaag atgccgaaaa agtattcggt atcaagccgg aaggttttga
     8221 agtagcccat accggtggcg aagtcaatgc cgctgaattt gagtcggttt gtaagcaatt
     8281 gatggctaaa tttccccggg caaaaaaagt aattattacc ctgagaggct ccatcaatgc
     8341 caatcacaat acctggagag gatgtttgta ttctgacgga tcattgaaaa tttcaaaagc
     8401 ttacgacatc acgcatattg tggacagagt cggcggaggc gattccttta tgggaggact
     8461 aatctacgga ttgctgacct atgaaggcga tgatcaaaag gcattagaat ttgctgtggc
     8521 tgcttcctgc ctgaagcaca ccatttacgg agatttcaat atggtatctg tagccgaagt
     8581 agagaagctg atgggcggag atgcttcggg cagggtttcc agataaacaa aaataatatg
     8641 gcaaaattta ctaaacaaca ggtaatcaat acgatttgca caagcggtat ggtacctgtc
     8701 ttctacaaca aagacataga agtgtccaaa caagtcatac tggcttgtta tcggggaggt
     8761 gttcgggttt ttgaatttac caaccggggc gactttgcac aggaagtctt caaagaactg
     8821 gtcaaataca ccgaaaaaac atgtccggaa atgattatgg gtgccggttc ggtcgttgac
     8881 gcaggtacgg cttctctgta tattcaactg ggagccaatt ttattgtagg ccctctgttc
     8941 aaccccgaag tggcaaaggt ttgcaaccgc aggctgattc cttacgctcc gggttgtggt
     9001 tctgtcaccg aaattggtta cgctcaggaa gccggttgcg atatatgcaa aatattcccg
     9061 gccggcaatg tgggtggccc ttcctttgta aagaacgtaa tggctcctat gccttggtcc
     9121 aagctcatgg ttaccggagg tgttacacct gaagaaagca acctaaaagc ttggtttgat
     9181 gcgggcgtaa cctgtgtcgg tatgggttct aacttatttc ctgccgacct gataaaatcc
     9241 gaaaattggg atggcattac caagctttgt gcagaagcaa tggctattat tcaaaaagta
     9301 aaaaagtaat ttatttacat atttttcaca cacaaattac atgaacatga gtaaagcaca
     9361 gactgtgacg caaaaaatgt cacaacacag atgggtcatt gtggcattgc tattttttgc
     9421 tacaactatc aattatttag acaggcaagt attatcactt acctgggatg aatttatcaa
     9481 accggaattt cactgggatg aatctcacta tggaacgatt acttcgttct tttctatttt
     9541 ttacgcaatc tccatgcttt ttgccggacg ctttgttgaa tggatgggaa ccaaaaaagg
     9601 ttatctttgg tccattggag tctggagtgt aggggcttgt ttacacgccg tttgtggtct
     9661 cgtaaccgaa gcacatgtag gtttacacag tgcagcagaa ctggctgccg ctacgggtga
     9721 tgtagccgtc gtaatcgcca ctttcagtat gtacagcttc ttggtagctc gaggtattct
     9781 tgctcttgga gaagcaggta acttcccggc agccatcaag gctacggcag aatactttcc
     9841 caaaaaagac cgcgcttacg caacctctat tttcaacgct ggtgcatcca taggagcatt
     9901 gtttgctccc ttgaccatcc cgccgctggc caaagcattc ggatgggaga tggcatttat
     9961 catcatcgga gcattgggtt ttatctggat gggtttttgg gtttttctct atgacgctcc
    10021 aagaaaaagt aagtttgtca actcacagga acttgaatac attgaacaag acaagcatga
    10081 agaaatagcc gaacaaaaga aaaaagcggc ggaagaagaa aaagttcctt tcctaaaaat
    10141 actctccttt aaacaaacct gggcttttgc actcggaaaa tttatgactg acggagtctg
    10201 gtggttcttc ttgttctgga ctccttctta cctctacacc caatttgata tcaagatgtc
    10261 ggagggactc ggtatggcct tgatttttac tctgtacgcc attgtaacgg tactttccat
    10321 cttaggtgga aaactaccca cggtctttat taatcgaggc ggacaaaatc cttatgcagc
    10381 acgaatgaaa gccatgttga tttttgcttt cttcccgttg gttgtattac tagcacaacc
    10441 cctcggtatg aagttcgccg ggttgggtca acatgctgcc tggattcccg tattgttgat
    10501 ttcgatagga tgttctgcac accaggcctg gagtgctaac ctgttctcaa cggttggaga
    10561 tatgtttcct catggtgcca ttgcaaccgt gaccggaatt gggggtatgg ccggaggtat
    10621 tggctcaatg attttacaaa aaagtgccgg caacttattc gtgtatgccg gagaaaccaa
    10681 catgacgttc tttggatttg tagggaaacc cgccggatac tttgtaatat tctgtatttg
    10741 tgccgtggct tatctgattg gatggattat tatgaaatcc ctggtgccga gataccaaaa
    10801 gattgttatt taattcagct ttctgccatt gaatgaacta cgataaaaga gcatctcaga
    10861 taatgacatg aaccccgaaa gttagtcggt ttaacattat taagaggtgg tctttgatag
    10921 gaataagagg ccgactaaaa aacaattttt agccggcctc tttgcttttc cccgatgatt
    10981 gcctgagttt gtcagcgaaa acggaatcaa cagaaaaaat cggaaactca ggaacactcc
    11041 tactcaaggc aagagttgaa atttatttca acacataagg aggatcgggc tctgcaatcg
    11101 ctgcttcaat tttgtgtggg aatcccatct ctatagcctt tcccaggcct ttgccctgca
    11161 cattgtaaat acttaatgca tcgccctctc tgatattcac attaatattc ctcaaaactg
    11221 ccttccttac attggccaaa cggatacccc ataaagcttc tccgctgata ttttccaaca
    11281 ccaggccttc gagaggtttg tcggggtgca ccgaagtccc gtctaccaaa tccttaacgt
    11341 ctctgacacg aacgtttttg aaagaaaaat tctttacggt gggaatccct tgcaatccgg
    11401 gcaccggcac ctgatcctgc aaaccgctgc ccattagatt gaaacggaga aaaccgcctt
    11461 gcatacccga tacgtcgaga ccttcggcac tgatatcttc gatgaaggct cccctaccag
    11521 gcctggattt aatataaatg gcatgggtac gagtgttctc aaaacggcaa ttttcaattc
    11581 tgactccccg aatacctccg gaggtttcac ttccaatacc gatgcaagca taaatgctgt
    11641 ctgaaagcct gcaattccgt atgtacacat cttcggtggt acgcatcaac atgttggctt
    11701 ccatccccct gcccgacttg atagcaatac aatcgtcgcc cgtttcgatg tcacatcttt
    11761 cgatcaggac atgtttacag gagtcaatgt ctataccgtc tccgttgccc cctgtagatc
    11821 tgatagtaag gtctttgaaa tgaatatttt cgcaataagt gggatgtatg caccacattc
    11881 tctggtaaga agtggagaaa tcgctaaaca gcagattttt acaattaatg ggctctatca
    11941 gggccggatg cctcagagga ttgtcactgg ttggcctgcc tcccaatgcg ggatggccaa
    12001 tgattttacc tccgcccacg ataccggtat agctggcatc cactgcatat atcagagccg
    12061 tacgaccttg tatccacttg ccttcccaac gcacctgcat cacggggtaa tcctcaaaat
    12121 ggggcgtgcc cctaagaatg gcctccg
//
LOCUS       DAOSGL010000027        12039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4479_length_12039_cov_4.004589, whole genome shotgun sequence.
ACCESSION   DAOSGL010000027 DAOSGL010000000
VERSION     DAOSGL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4479_length_12039_cov_4.004589"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PKY68_01805"
     CDS             <1..792
                     /locus_tag="PKY68_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH23806.1"
                     /translation="RNKILSDKLPAYLSYQVNFSKKLEEKHSFLLSGGNYQACFYPVY
                     RYTRFSLQTSRQLSLEYTYEGEQLFIQAAIYGKQEESPTYYGPEYKMKILQRDIRGLE
                     CSAEYEWNRFRASLSYTLLDAHIKSDSTWHKAENNLPCFLKAYLSYTGKHLLSLSCSG
                     IYRTGLRYTPVESGRYVVEAYAFEPQHGAYNSRRYDDYFRLDLSLNQVIFGKNQRRLV
                     VFATVGNVLNRKNQKARLYSPDYSDTIGYSHYQGRTYYLGAQVSF"
     gene            complement(782..1960)
                     /locus_tag="PKY68_01810"
     CDS             complement(782..1960)
                     /locus_tag="PKY68_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOH23807.1"
                     /translation="MAVNRDTSSFFRLVFFLVALIIIAASTWFSNDLGKELAAEEQKK
                     IELWAEAIRLLAQEPQPGEEDVRFDYTLTLKVIEGNTNIPVILTDGDNNVQSYANLNL
                     SKKHPEADLEKKAAHLIQKNRHIQVKLDENTYQYVYYDDSDLLKKLRYFPFVQLGVMF
                     VFLAVTLVSLTVFKRSEQNRVWVGLSKETAHQLGTPISSLLAWAELLKLKDIDPALLA
                     EMEKDTQRLRTIAERFSKIGSRPDAEYHALAPVVENAVNYLRGRSSQKVIFRTHFPKE
                     ELPVLLNIPLFEWVVENLCKNSIDAMNGEGQIDIVAAEVKKQVLIDICDSGKGIPKKH
                     FKAIFRPGFTTKARGWGLGLSLVKRIIEEYHKGKIFVKSSDPGKGTCIRIVLRKALKS
                     "
     gene            complement(1991..2758)
                     /locus_tag="PKY68_01815"
     CDS             complement(1991..2758)
                     /locus_tag="PKY68_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOH23808.1"
                     /translation="MKIISYNLNGIRSALNKDLTAFLQEEAADVVCFQETKAQPDQID
                     SSLFWEIGYPHCYYHSAVKKGYSGVAILSKREPDRIVVGMDNHKYDSEGRILRADYGD
                     FSILCVYVPSGSMGDVRQQFKMEFLSDFIDFVGELRKERPNLLICGDFNICHKPIDIN
                     HPERHQNASGFLPEERAWMDDWAQTGLIDTFRQFDPSPEKYTWWSFRANSRARNLGWR
                     IDYHWITTTLESNLKGAAILPEAMHSDHCPVVVELDL"
     gene            complement(2768..3547)
                     /locus_tag="PKY68_01820"
     CDS             complement(2768..3547)
                     /locus_tag="PKY68_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH23809.1"
                     /translation="MIEVRDVCKSFVANELVLKHISTTFHSGKINQIIGRSGSGKTVL
                     LKCIVGLHTLDSGDILYDGRSFTAMSKKEKMQLCSEIGMLFQGSALFDSLTIIENVLF
                     PMQMFSEKSEKEQQDRARFCLERVGLYNVFDMYPAELSGGMQKRAAIARAISLNPKYL
                     FCDEPNSGLDPLTASKIDELIVELTKEFQMTTIINTHDLNSVLSVGEHIVFIHKGEKA
                     WEGPKEKLFVSNCSELDDFIFASEMYQKFRTTLVSEQSGQI"
     gene            complement(3544..4284)
                     /locus_tag="PKY68_01825"
     CDS             complement(3544..4284)
                     /locus_tag="PKY68_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH23810.1"
                     /translation="MNPLYHVGRYSQLMYRCFSKPQRAGVFVSQTLKEIDRLGVSSLF
                     ITLIVSLFMGAVLTIQTQNNIENPLLPDYTVGLVTRDTLLLEFCSMVLCLFLAGNIGS
                     NIASQIGTMRISEQIDAMDIMGVNSANYIILPKIVAVVLYVPVLVVFSMFTGMLGGYL
                     IAAFTDIITTSKFIYGLQYWFIEWYVYYSLIKAAVFAFIIASVSAYWGYFVRGGTLEV
                     GKASTRAVVTCSVLILLADVVLTQILLL"
     gene            complement(4288..7857)
                     /locus_tag="PKY68_01830"
     CDS             complement(4288..7857)
                     /locus_tag="PKY68_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOH23811.1"
                     /translation="MKRQTFFRTSLIGLLFFLSFNLFAKLNNYPVPDRDTVPAFPGAE
                     GAGKFTSGGAGGQVYIVNKLDDDGSEGTLRWALEQEGRRTVVFAVDGTISLDSHLLVT
                     NGDLTIAGQTAPGQGICLKNYPLLISADNVIVRYIRSRMGDERRTQDDAMGAMRQKNI
                     IVDHCSMSWGTDECASFYNNQNFTLQWCIISESLANSIHQKGEHGYGGIWGGQPASFH
                     HNLIAHHTSRTPRFNGSRQTRRPQDEKVDFRNNVIYNWGPIDGGYAGEGGFYNLVNNY
                     YKPAYATVQNTKLVNRIFAPWVGRVTPEYPRGETGRFYVSGNYFDDGSPALDQKHKSL
                     LAAVNRDNWLGIHPNEDFKGELNELRLDKPVEMAEINTQNAREAYLAVLAGAGASLHR
                     DKIDRRIVEETRTGTCINYLSGGTGIVDSQSQLGGWPELKTAPAPADSDADGMPDEWE
                     KLKGLDPNNPADASLYQLSPSYTNLEVYMNSLVCRPWEDRDLPVDERIESLVKEMSLE
                     EKCGQLVNGAPGIERLGILPYDWWNEALHGVGRNGRATVFPQAIGLAATFDEDLIFRV
                     ASAISDEARAKFNASLAMERYEQYSGLTFWTPNINIFRDPRWGRGQETYGEDPYLTSR
                     LGTAFVRGLQGDNPDYLKTAACVKHYAVHSGPEALRHEFDAHPSARDFYETYLPAFET
                     LVKEAKVEAVMGAYNRVYGLPACGSGLLLDTILRQQWGFEGHIVSDCGAIRDFYTTHK
                     VVQTPEQAAAMAANVGLNVNCGSVYRYLGAAIEQGLVTETTIDNLIRPLLRTKIKLGF
                     FDPSWANPYNSIGPEVVESPAHLALTREAAGKSMALLKNDKGVLPLDKNIRSLYITGP
                     FAADANVLLGNYYGLSSSLSTFLEGIVAKLNPGSKCDYKPGIMMEGENRNPIDWTTGD
                     AKKADAIVVVLGITPLKEGEEGESIASAHKGDRPDLNLPENQVNYLKKLRHNNNKPII
                     VVLTGGSPITMPEVTELADAILFAWYPGQEGGNALGDILFGDVNPSGRLPITFPKSVD
                     QLPPYEDYSMKERTYRYMTQDPLYPFGYGLSYTTFAYKDARLESASVKRGQSLRMEIR
                     VENTGNRPGEEVVQFYLSKPGAGSSGPFSSLIGIRRVKLEPGQSQLLEFEISPDAMTS
                     IDDKGKKQVLKGDYTLIVSGAAPVKRSAELGVDNRIDLKFRLK"
     gene            8079..10643
                     /locus_tag="PKY68_01835"
     CDS             8079..10643
                     /locus_tag="PKY68_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23812.1"
                     /translation="MKKSSLITLIALAAVTIFLSWWIYVPSNSFVYIYSSLNQEDTIV
                     FEGDHILYNNEKIVLGEKAFFVDGNLSNRVVKRHPFVYNSFNEAMKDIREGSPEEAMT
                     LYIAPYVYWIDDPDDPSERVGENGGIPYGLVINESNLHFYGLSKDPQHVVLACNRGQT
                     QGAVGNFTMFRFNGNNIRCENLTMGNYCNVDLVYPLKTSLNRTKRSSAITQAQLALCN
                     NSDKIFASNCAFISRLNTCPFVGSKRAFFVDCRFESTDDALCGNGVYLNCDLDFYSSK
                     PFWSTHGTGAAFLNCDFNVITRNAQYLTKVGSQVALVDCRFKNNGDSLYLGWTQYPKD
                     HMRCYQYNVSLNGRAVLFQSDRPHLTVDMLDQEVLKAYRFKYEDKLIYNTYNLLRGDD
                     DWDPQGIKDIVAAASQQDDFDYSTVPVQLSVIPQFVEVQTGKKADTLFFAVKRFGDIP
                     VEETVKWSINSEDAPYLYIRKLKNGNCLLTGSNRGDQIRTVVVEARHSSGLRGASVVR
                     VLPNILPAPIFTKKPVLSAPDQGVIKLSYSLNLQHRADHSLITWYRCKDARGSEAIPV
                     AVSRLNQPEYRYNLSAGDVGYYLQAKIEPKHIRSHPGAAFTVCSAEPVTKEDVLNAQI
                     ITTDFQNFPADTQKQILPGFWTVDAFKPADTEAYHWQANSEGAWFYGTAEGGANGTGF
                     LQGQRGARLLYTPVDGNYGNMGVNIIADPCKTAGQGFGSATGQYMDIYIKFDTKSLSG
                     YGLRIVRTPKYANAVDFVLMEYNNGVSREISEAISATCFLTNCSIKLATEGNLLKADI
                     STTTPKPYESNPDLPHEVKLTAEITENNFGGSGIQHTGSAGSGATMLHKMEIIYQE"
     gene            10662..>12039
                     /locus_tag="PKY68_01840"
     CDS             10662..>12039
                     /locus_tag="PKY68_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH23813.1"
                     /translation="MSIRKICTICLLLLTSFTGFTQGFGNRQLINKGWYFNLGDLKYG
                     GAEFLDHSSWRQVQLPHDWSVEGTPSPQWASCTGYLPGGIAWYRKDLDIPSDLEGQKV
                     FIYFEGVYNNSEVFINGKWIGKRPNGYISFMYDLTPYLNFGGRNVLAVRVDHQDYADS
                     RWYTGSGIYRDVWLVYAPKTHIDLWGVSYQTEIKGKSALVKIQTEVLNEDLKEMSCTL
                     RQNIYDSKDKLIGSTQGKMLIPAGEKRKLEQNITVQNPELWDLNSPHLYRLETTLLHN
                     NKVLDQTDTRVGIRSIRFDPNQGFSLNGKSLKLKGICMHHDAGCLGAAVDREVWRRRL
                     IQLKSLGCNAIRTSHNPQATCVYELCDELGLLVNNEAFDEWEYPKKKWIEGWNVGTPG
                     FQGSALYFREWCKRDLADMVRRDRNHPSVIMWSIGNEVDYPNDPYSHPVLDKEGIGQQ
                     HVRGYLPGQ"
BASE COUNT         3191 a         2939 c         2789 g         3120 t
ORIGIN      
        1 agaaacaaaa tcctgtcgga caaactccca gcctaccttt cgtatcaagt aaatttcagc
       61 aagaagcttg aggagaagca cagttttctg ctcagcggcg ggaactatca ggcctgtttt
      121 tatcccgttt accgttatac ccgtttctcg cttcaaacat cgcggcaatt gtcgctggag
      181 tacacctacg agggagagca gctttttatt caggcggcaa tatacggcaa acaggaagag
      241 tcgcctactt actacggtcc ggaatacaaa atgaaaatac tacaaagaga tatacggggc
      301 ctggaatgct cggcggaata cgaatggaac agattccggg cctctctttc gtatactttg
      361 ctcgatgccc acatcaaatc agacagcact tggcataaag cagaaaacaa cttgccctgc
      421 tttctgaaag cgtatttaag ttataccggc aaacacttgc tgagcctttc ctgcagcgga
      481 atataccgga caggattacg ctataccccc gtggaaagcg gtcgttacgt tgtagaagcc
      541 tacgcgtttg aacctcaaca cggagcttat aattcccgga gatacgacga ttatttcagg
      601 ctggatctaa gcctgaatca ggtaatattc ggaaagaatc aacgcaggtt ggtagtcttt
      661 gctaccgtgg gaaacgtttt gaaccggaag aatcagaaag cccgtctcta ttcgcccgat
      721 tattcggata ccataggcta ctcacactat cagggccgaa cgtattattt gggagcccaa
      781 gtcagctttt gagcgctttg cgcaatacga tgcggatgca ggtgcccttg cccgggtcgg
      841 agcttttgac gaagattttg cctttgtgat attcttcgat gatgcgtttc accagcgaca
      901 aacccagccc ccagccgcgt gccttggtgg tgaaacccgg acggaaaatg gctttgaagt
      961 gttttttggg tatgcctttg ccgctgtcgc aaatatcgat taggacttgt ttttttactt
     1021 cggccgctac gatgtcgatt tgcccctcgc cattcattgc gtcgatggag tttttacaca
     1081 aattttcgac cacccattca aacaaaggta tattcagcaa taccggcaat tcttctttgg
     1141 ggaaatgcgt ccggaaaatc accttttgcg aacttcgtcc cctgaggtaa ttcactgcat
     1201 tttccaccac cggggccagt gcatggtatt cggcatcggg cctggatcct attttggaaa
     1261 aacgttcggc aatggttcgc aggcgctggg tgtctttttc catttctgcc agcaaagcgg
     1321 gatctatgtc tttgagcttg agcaattccg cccaagccaa aagcgacgaa atcggtgttc
     1381 ccagttggtg ggccgtttct ttggacagac ccacccatac acggttttgc tccgatcgct
     1441 tgaacactgt gagcgaaacc agtgtgacgg ccaaaaaaac gaacatcact cccaattgta
     1501 caaaaggaaa ataacggagt tttttcaata aatcggagtc gtcgtaatag acgtattgat
     1561 aggtgttttc gtctaattta acctggatat gcctgttttt ttgtatcaaa tgggctgctt
     1621 ttttctccag gtcggcctcg ggatgttttt tcgacaaatt caaattggca tagctttgaa
     1681 cattgttgtc accgtcggta agaataaccg ggatattggt gttgccttcg atcactttaa
     1741 gagtaagggt gtaatcgaaa cgaacatctt cttctccggg ctggggttcc tgggccagaa
     1801 gccgtatagc ctcggcccac agctcgatct ttttttgttc ttcggcggcc agctctttac
     1861 ccaggtcgtt ggaaaaccag gtagatgcgg cgataatgat cagggcgacc agaaaaaaaa
     1921 caagacggaa aaaagaggaa gtatctcggt tgacagccat ggttttacgg attttttaaa
     1981 aacagtttgg ttaaagatcc agttccacca ccaccgggca gtggtcggaa tgcatggcct
     2041 cgggtagtat ggcggctcct ttcagattgg attccagcgt tgttgttatc cagtggtaat
     2101 cgattctcca gcctaaattt ctggccctcg aattggctct gaagctccac caggtgtatt
     2161 tttcgggcga gggatcgaac tgcctgaagg tatcgatcag gccggtttga gcccaatcgt
     2221 ccatccaggc tctttcttcg ggcaggaagc cggaagcgtt ttgatgtctt tcggggtgat
     2281 tgatgtctat cggtttgtgg caaatattaa agtctccgca aatcagcaaa ttaggacgtt
     2341 ctttacgcag ctctcctaca aaatcgatga aatcggacaa aaattccatt ttgaattgtt
     2401 gtctcacatc gcccatactg cccgagggca catagacaca aagaatactg aaatcgccgt
     2461 aatcggctct caatatccgg ccttcgctgt cgtatttgtg gttgtccatg cccaccacaa
     2521 tccggtcggg ttctcgtttg gataaaatgg ccacgccgct gtagcctttc tttactgcgg
     2581 aatggtaata acaatgggga taaccgattt cccagaacag ggaagaatcg atttgatcgg
     2641 gctgagcttt ggtttcctga aaacagacca cgtcggctgc ttcttcttgt aaaaaggccg
     2701 ttaaatcttt gttcagggcc gatcgaatac cgttcaggtt gtaagagata attttcatgc
     2761 tgatcagtta aatttgtccc gattgctcgc tgacaagggt ggttctgaat ttttgataca
     2821 tttccgaggc aaaaataaag tcgtccagtt cggagcaatt cgaaacgaaa agtttttctt
     2881 tgggtccctc ccaggctttt tcccccttgt ggataaacac gatatgctcg cccacgctca
     2941 agaccgaatt taaatcgtgg gtattgataa tggtggtcat ctgaaattcc ttggtcaatt
     3001 caacaatcaa ttcgtcaatt ttgctggctg tgagaggatc caggcccgaa ttgggttcat
     3061 cgcaaaacaa atatttcgga ttcaacgaaa tagcccgggc aatggcagcc cttttttgca
     3121 ttcctccgct caattccgca ggatacatat cgaatacatt ataaaggcca accctctcga
     3181 ggcaaaaacg ggctctgtct tgttgctctt tttcgctttt ttcggagaac atttgcatgg
     3241 gaaagagcac gttttcgata atggtgagcg aatcaaacaa agccgaaccc tgaaagagca
     3301 taccgatttc gctgcacaat tgcatctttt cttttttact catggccgta aaacttctgc
     3361 cgtcgtaaag tatatcgccc gaatcgagcg tatgcaggcc cacgatgcat ttcaacaaaa
     3421 ccgttttgcc cgagccgctt cgcccaatga tctggttgat ttttccgctg tgaaatgtgg
     3481 tgctgatatg ttttaataca agctcgttgg caacgaaaga tttgcataca tccctgactt
     3541 cgatcatagc agcaaaattt gagttaacac cacatcggcc agcaggataa gcacactgca
     3601 agtgaccacg gccctggtgc tggccttacc gacctcgaga gtgccaccac gtacaaaata
     3661 gccccaatag gccgatacgg aggcaataat aaaggcaaaa acggccgctt ttatcagaga
     3721 ataatacaca taccattcta taaaccaata ttgcaggccg taaataaatt ttgaagtagt
     3781 aatgatgtcg gtgaaggcgg caatcaggta tccgcccaac atcccggtaa acatgctgaa
     3841 cactaccaaa acgggaacgt ataaaaccac ggctacaatc ttgggcaaaa taatgtagtt
     3901 ggcagaattg acacccataa tatccatagc gtcaatttgt tccgaaattc gcatggtccc
     3961 tatctgagaa gcaatattcg accctatatt ccccgcaagg aacaaacaca aaaccatgga
     4021 acaaaattcc agcaataagg tatcgcgggt aaccagcccc acggtataat cgggcaacag
     4081 aggattttcg atgttgtttt gggtttgaat ggtcaggacg gcccccataa acaaggaaac
     4141 gatcagggta ataaaaagtg agcttacacc caggcgatcg atttctttaa gtgtttgact
     4201 aacaaacact cccgcccgtt gcggcttaga aaaacacctg tacataagtt gggaatacct
     4261 cccaacatgg taaagtggat tcatagttta ttttagtcga aatttcaaat ctatgcgatt
     4321 gtcgactccc agctctgcac ttcgcttgac gggagctgct cccgatacaa tcagggtata
     4381 atctcctttg agaacttgct ttttcccctt atcgtcgatc gaagtcatgg catcggggct
     4441 gatttcaaat tccaggagtt gagactgacc gggctccaat ttaacccgcc tgataccgat
     4501 cagggaactg aaggggccgc tgcttccggc tccgggtttg ctaaggtaaa actgtaccac
     4561 ttcttcgccc ggacggttac ctgtattttc aactctgatt tccatccgca gggactggcc
     4621 tcttttgacg gaagctgatt ccagccgggc atccttgtaa gcgaacgtag tataactcag
     4681 cccataacca aaaggataca agggatcctg agtcatatag cgataggttc tttctttcat
     4741 ggaataatct tcgtagggag gcaattgatc taccgactta gggaaagtaa tcggcaatct
     4801 gccggaagga tttacatctc caaataaaat gtcgcccaag gcgtttccac cctcctggcc
     4861 cggataccag gcaaacaaaa tagcatcggc cagctctgtt acttcgggca tggtaatggg
     4921 actgcctccg gtcaggacta caataatggg tttattgttg ttatgacgca gttttttcaa
     4981 ataattcacc tggttttcgg gcaaattcaa atcggggcga tcgcctttgt gtgcagaagc
     5041 tatcgattcc ccctcctcgc cttctttgag tggggtaatt cccaacacaa cgactatggc
     5101 atcggctttt ttggcgtcac ctgtggtcca gtctatggga ttccggttct cgccttccat
     5161 cattatgccg ggcttgtaat cgcatttgct gcccgggttc aattttgcca caattccttc
     5221 taagaaagtg gaaagcgaag aactcagacc gtaataattt cccaacaaga cattggcatc
     5281 ggcggcaaag gggcctgtta tgtacagact gcgaatattt ttatccaaag gcaacactcc
     5341 cttgtcgttt ttgagcaatg ccatagactt gcctgctgct tctctggtca atgccaggtg
     5401 agcgggcgac tcaaccactt cggggcctat gctgttgtaa ggattggccc atgaggggtc
     5461 gaagaagcct aattttattt tggtcctcaa caaaggacgg attaaattat caatggtggt
     5521 ttccgttaca agcccttgtt cgatggccgc gcccaaatac cggtaaaccg aaccgcaatt
     5581 aacgttgaga ccgacatttg ccgccatggc ggccgcttgt tcgggagttt gtaccacctt
     5641 gtgtgtggta taaaaatctc tgatggctcc gcaatccgac acgatatgtc cctcgaaacc
     5701 ccattgttgc ctgagtatgg tgtccaaaag caagccgctg ccgcaggccg gcaaaccata
     5761 gacccggttg tatgctccca tcacagcttc cactttggct tccttgacca gggtttcgaa
     5821 tgccggcaaa taagtttcgt aaaaatccct ggcggaggga tgagcgtcga attcatgacg
     5881 taatgcttcg gggccggagt gtacggcata atgtttgacg caggctgccg tcttaagata
     5941 gtcgggattg tctccctgca aaccgcgcac aaaagccgtt cccaaacgcg aagtcagata
     6001 agggtcttcg ccgtaggttt cctgccccct gccccagcgg ggatctcgaa aaatatttat
     6061 gttgggagtc caaaaagtca ggccggagta ttgctcgtaa cgctccatgg ccaaagaggc
     6121 attgaacttg gcccgggctt cgtccgaaat agccgaagcc actctgaaaa tcaagtcttc
     6181 gtcgaatgtg gctgccaaac cgatagcctg aggaaaaacg gtagccctgc cgtttcggcc
     6241 gacaccgtgt aaagcttcgt tccaccaatc gtagggtaat atgcccagac gttcgatacc
     6301 cggcgctccg ttgaccaact ggccgcattt ttcttcgaga ctcatttctt tcaccagtga
     6361 ttcgatgcgt tcgtccaccg gcaaatccct atcctcccag ggccggcaaa ccagggagtt
     6421 catatacacc tctagattgg tataagacgg actcagctga tacagactgg cgtctgcggg
     6481 gttattggga tccagcccct taagcttttc ccattcatcg ggcataccgt ctgcatcgga
     6541 atctgccgga gccggcgctg tcttgagttc gggccagccg cccagttggc tttggctgtc
     6601 cactataccc gttccgccac ttaagtaatt gatacaagta cctgttcggg tttcttccac
     6661 tatacgtcta tcgatcttgt cacgatggag agaagcgccg gcaccggcca gcaccgccag
     6721 ataagcttcc cgggcgtttt gcgtattgat ttcggccatt tcgaccggct tatccaacct
     6781 taattcgttc aactcccctt tgaaatcttc gttcggatgt atgcccaacc agttgtcccg
     6841 attcacggct gccagcagag atttatgctt ttggtccagg gccggggaac cgtcgtcaaa
     6901 ataattaccc gacacataaa accggccggt ttcacccctg ggatattcgg gagtgactct
     6961 gccgacccag ggtgcaaaaa tgcgattgac cagttttgtg ttctgcacag tggcataggc
     7021 cggtttataa taattgttta ccagattgta aaagcctccc tcacctgcgt agccgccatc
     7081 gatcggtccc cagttgtaaa tgacgttatt gcggaaatcc actttttcgt cttgcggtct
     7141 gcgagtttgc cggcttccgt taaaacgggg agtacgactg gtgtggtgag caatcagatt
     7201 gtggtgaaaa ctcgcgggtt gtccgcccca aatgccgcca taaccatgct ctcctttttg
     7261 gtgaatggaa ttggccaggc tttcacttat tatgcaccat tgcagggtaa aattttgatt
     7321 attgtaaaag gaggcacatt catcggtacc ccagctcatg gagcagtgat ccacaataat
     7381 attcttttgg cgcatagcac ccatggcatc gtcttgagtt ctcctctcat ctcccattct
     7441 ggagcgtata taacgaacaa tcacattatc ggcagaaatc agcaaaggat aatttttcag
     7501 acaaatacct tggccgggag ccgtttgtcc ggctatggtt aaatcgccgt tagtaacaag
     7561 cagatgacta tccaacgaaa tagttccatc tacggcaaaa accaccgttc gtcttccttc
     7621 ctgctccaaa gcccacctaa gggttccttc cgatccgtcg tcgtccagtt tgttaacgat
     7681 ataaacttgt cctccggcgc ctcctgaagt gaatttaccg gctccttcgg ctcccgggaa
     7741 agccgggacg gtatccctgt cgggaacggg ataattgttt aactttgcaa acagattaaa
     7801 actcagaaaa aaaagcagtc ctatcagact tgtgcggaaa aaagtttggc gtttcatgct
     7861 tattgattta tattcagact tttatttgca ttgggagaca aaaatagctc tttttgcctt
     7921 taaataaagg aatgaggcaa aaatatcaca tttattaaat caagttcagg aacaaatcga
     7981 aaatagtttc aataatgaaa aatagcttat aaaccggccc aattcaacaa caaaccataa
     8041 tatataacta tatttgcaaa ccttaaaact ccggaaaaat gaaaaaatcc agcctgatca
     8101 ccctaatcgc gttagcagcc gtcaccattt ttttaagctg gtggatttat gtacccagta
     8161 attcctttgt atacatttac tcctctctta atcaagagga tactattgtt ttcgaaggcg
     8221 atcatatcct ctacaataac gaaaaaatcg tattggggga gaaggctttt ttcgttgacg
     8281 gcaatttgtc caacagggtc gtcaaacgcc atccctttgt gtacaattct ttcaacgagg
     8341 caatgaaaga catccgcgaa ggcagccccg aagaagcaat gaccttgtat attgctcctt
     8401 atgtttattg gatagacgat ccggacgatc cttcggaacg agtcggtgaa aatggaggaa
     8461 tcccttacgg cctggttatt aatgaaagta atttgcattt ttacggcttg agtaaagatc
     8521 ctcaacatgt ggttttggcc tgcaacagag ggcagacaca gggagctgtg ggcaatttta
     8581 ccatgtttcg tttcaacggt aataacattc gttgcgaaaa tctgactatg ggaaattatt
     8641 gcaatgtaga cctggtttac ccgctcaaaa catctctcaa cagaacgaaa cgctcttcgg
     8701 ccattaccca ggctcaactg gcattatgca acaacagcga taaaattttt gctagcaatt
     8761 gtgcctttat aagccgatta aatacctgtc cttttgtagg cagcaaaaga gcttttttcg
     8821 ttgattgtcg tttcgaaagt accgacgatg ccttgtgcgg caacggggtc tacctgaatt
     8881 gcgacctcga tttttacagt tcaaaacctt tctggagtac acacggcacg ggtgctgctt
     8941 ttttgaattg cgattttaac gtaattaccc gaaacgccca atacctgacc aaagtaggca
     9001 gccaagtggc tttggtcgat tgtcgcttta aaaacaacgg ggattccctc tatttgggat
     9061 ggactcaata ccccaaagac catatgcgat gttatcaata caatgtgagt ttaaacggcc
     9121 gggctgtgct gtttcaatcc gaccgtcctc acctgacagt agatatgctc gaccaggaag
     9181 tattgaaggc ttaccgtttt aaatacgagg ataaactcat ctataacacg tataatttgc
     9241 tccgcggcga cgacgactgg gatccgcagg gcattaaaga tatagtggcc gcggcctctc
     9301 agcaagacga tttcgattat agcacggttc ccgttcaact gagtgtaata ccgcagtttg
     9361 tcgaagtcca aaccgggaaa aaagcagata cacttttctt tgccgtcaag cggtttggag
     9421 atatcccggt cgaagaaaca gtcaaatggt ctatcaactc cgaagatgct ccttatttgt
     9481 acattcgcaa gctgaaaaac ggtaattgcc tgttgaccgg gagcaaccgg ggcgatcaga
     9541 tacgaaccgt tgtggtggaa gccagacact cttccggact tagaggagct tctgtggtca
     9601 gggtcttacc gaacattctt cctgctccca tttttacaaa aaaacctgtg ttgtctgccc
     9661 ccgatcaggg cgttataaag ctaagttaca gtctgaatct tcaacacagg gcagaccact
     9721 cccttattac ctggtaccgc tgcaaagacg cccggggctc ggaggccatt cctgttgccg
     9781 tcagtcgcct gaaccaaccg gaatataggt acaatctcag tgcaggtgat gtcggttatt
     9841 accttcaggc taaaatcgag cccaagcaca taaggagcca tcccggggct gcctttacag
     9901 tttgcagcgc cgaaccggtt acaaaagaag atgttttgaa cgcccaaatt attaccaccg
     9961 attttcagaa tttccccgcc gatacgcaaa agcaaatcct gcccggtttc tggacagtcg
    10021 atgcctttaa accggccgat accgaagcct accattggca agcaaattca gagggggcct
    10081 ggttttacgg aactgccgaa ggcggagcaa acggaaccgg ttttctgcaa ggtcagagag
    10141 gtgcccgcct gctttacacc ccggtcgacg gtaattacgg caatatgggg gtaaatataa
    10201 ttgccgaccc gtgcaaaaca gccggccagg gctttggcag cgccaccggt caatacatgg
    10261 atatttacat aaaattcgac acaaaaagcc tgagcggtta cggcctgcgt atagttcgga
    10321 ctccaaaata cgccaacgca gtagacttcg tactgatgga atacaacaac ggggtcagcc
    10381 gcgagatctc cgaagcaatt tcagctacct gcttcctgac gaattgcagc ataaaactcg
    10441 caacggaagg aaacttgttg aaagccgata tttccaccac tacccccaaa ccctacgaaa
    10501 gcaatcccga cttgcctcac gaagtgaagc tgacagcgga aatcaccgaa aacaactttg
    10561 gaggaagcgg tatccagcac accggatctg caggaagcgg tgccactatg ttgcacaaaa
    10621 tggaaataat ttatcaagaa taaccttaaa tccacacccc tatgtcaatc cgtaagatat
    10681 gtacaatttg cctgttactg ctgacctcct ttaccggctt tacacaaggt ttcggcaatc
    10741 ggcagctcat caacaaaggc tggtatttta acttgggcga tctgaaatac ggtggggccg
    10801 agtttctgga tcattcttcg tggagacagg tgcaactgcc gcacgactgg agcgttgagg
    10861 gcactcccag tccgcaatgg gccagttgca caggctattt gcccggaggc atagcctggt
    10921 atcgcaaaga cctcgacatc ccctccgatc tggaaggaca aaaagttttt atctatttcg
    10981 aaggcgttta caacaacagc gaagttttta tcaacggcaa atggataggc aaacgcccta
    11041 acggctatat ctcgtttatg tacgatctga caccctatct caattttggg ggcagaaacg
    11101 tgctcgctgt cagagtcgat caccaagact atgccgattc gagatggtat acgggttcgg
    11161 gtatttaccg cgatgtctgg ctggtgtatg ctcccaaaac ccatattgat ctttggggtg
    11221 ttagttatca aacggagata aaagggaaaa gtgccctcgt caagattcaa accgaagtac
    11281 tcaacgagga tttgaaggaa atgagttgta ctctccgcca aaacatatac gacagcaaag
    11341 acaaactgat aggcagcacc caggggaaaa tgctgatacc ggcaggagaa aaacgcaagc
    11401 tggaacaaaa tataacagta caaaatcctg agctctggga tttgaattct ccccatctct
    11461 atcgcctgga gaccacatta ttgcacaaca acaaagtttt ggatcaaacc gacaccagag
    11521 tcggcatacg aagcatacgt tttgatccca accaaggttt cagtctgaac ggcaaatcac
    11581 taaaactcaa aggtatctgc atgcaccacg atgccggctg cctgggagct gccgtagacc
    11641 gtgaagtgtg gcgaagaagg cttattcaac tcaaatcgct gggctgcaac gccatacgca
    11701 ccagccataa ccctcaggct acctgtgtct atgagctttg cgacgaatta ggcttattgg
    11761 tcaacaacga agccttcgat gaatgggaat atccaaaaaa gaaatggata gaaggctgga
    11821 atgtgggcac tcccggtttt cagggttctg ctctttactt cagagaatgg tgtaagcgag
    11881 atctggcaga tatggtcagg cgcgacagga atcaccccag tgttatcatg tggagtatcg
    11941 ggaatgaggt tgattatcct aacgatccct actcacatcc cgtactcgat aaggaaggta
    12001 tagggcaaca acacgtcagg ggctacttgc ccggccaac
//
LOCUS       DAOSGL010000028        11568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4687_length_11568_cov_4.099453, whole genome shotgun sequence.
ACCESSION   DAOSGL010000028 DAOSGL010000000
VERSION     DAOSGL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4687_length_11568_cov_4.099453"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKY68_01845"
     CDS             complement(<1..646)
                     /locus_tag="PKY68_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH23814.1"
                     /translation="MTVDYSTEYRESILIKNILLGRKSQDVLIEGNRFTRIEDKIDLS
                     ANRVVEGKGKALLPPFYNAHTHAAMSLLRGCAEDMHLTEWLRDKIWPLEAKLQGDDHY
                     VGTRLAILEMIKSGTVFFSDMYFNVPDTIRAVEEMGIRAAVGVTFLESNGAKYIERGL
                     RLLENLPETSSRVQITIAPHAVYTVSEDLFKRLAAMARERSLIIHTHLAETRQEV"
     gene            732..2798
                     /locus_tag="PKY68_01850"
     CDS             732..2798
                     /locus_tag="PKY68_01850"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOH23815.1"
                     /translation="MKRCFLFLLLVGLLWPTTPSAQNRSCRLFTFDADQMFNKYLLRQ
                     VYEQYLERDAFFCSGFSAAEAREYGNKAKQAYLDLLGPMPNRTAVAVEHCGNHNFGAF
                     SLEKIIYQSSPNRRVTANLYLPGQAGKHPVVLMLCGHGAQGKQTPFYQYQAALYAANG
                     IATMVVDPPGQGERFQLIDKDGNNPARGGSGTNEHTLLLMNSLIVGESMVKDELWDNL
                     RAIDYLCSRPDIDTSRIGCFGFSGGGTQAEYLLAFDTRIKVGGIGGYFTQRRRSMEVI
                     ASQDGCQHLPGEGLKALEQVDFVTAFAPKPLIIINGAYDFVDRIGAEAGYRELKKFYA
                     ACGRPEQVLLYTGDDGHAFGEEKQRLVLAWFLKYLCNKEQKGQTFRNPSSKADYASVK
                     AFAEETGITPAYDFEPIPANEMLCTPKGNLSTYLPKGELLTPARNQQIWQEQAGSRAK
                     FHTLSPEAQNRQVISLAGINPGVEVRVEKAGSTVLEGIRFERYILHAENETPLPCLLL
                     LPDKPQRDAVRIILHEQGKCLIADSCIQLCQNSGEIILLADLRGLGESRDKPERNESK
                     YFNTEYSVGMMSLHIGKTLVGQRATDILMLASFIGQHETLKNCRDLQLEAYGKCVPPA
                     LHAAFLLDNLREIRLHSELPSWEYLLEHPTTKEQLSYIVPKASKYYDLPDLQKALIAR
                     KISVIY"
     gene            2826..3587
                     /locus_tag="PKY68_01855"
     CDS             2826..3587
                     /locus_tag="PKY68_01855"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOH23816.1"
                     /translation="MDIEKKRFKLSFYVPTLLVFLLALVQALQSGLQESWYRLGIYPR
                     EISSLPGIITQVFIHENWAHFFNNAVPLFVLSWCFYCFYRELFWKGSLLLWLSSGLFT
                     WLIGRANWHIGASGLIYAYSFFLFFSGLIREHKPLMAISFVVVFLYGSTFWNMFPWGE
                     FNRDISWEGHLSGAMAGLLWALLLRKQGPQAPPPPEEEEDEEEAINSELDSTAEELAE
                     KNPDKDEVKVTGENSSRQTAINVFPNAPEQQQPTA"
     gene            complement(3541..4608)
                     /locus_tag="PKY68_01860"
     CDS             complement(3541..4608)
                     /locus_tag="PKY68_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOH23817.1"
                     /translation="MNLYPKLILDALSKVRYPGTSQDLVSSGMVEDNIRIEGNKVSFS
                     LIFEKQNDPFIKSVVKAAETAILTYVGEEVDIKGNIKVVVRPAAPVKEEEALLPGVKN
                     VVVIASGKGGVGKSTITTNLAVALAAEGYRVGLLDADIFGPSIPKMLDMEEVRPVMEN
                     ILGRDRIIPEEKYGLKVLSIGFFVQKENAIVWRGPMASNALKQLIGDTYWGELDYFLI
                     DLPPGTSDIHLTLVQNLKLSGAIVVTTPQQVALTDARKAVDMFQNEKINVPILGLVEN
                     MSWFTPAELPQNRYYLFGKDGGAELAKERGVPLLGQIPLVQSVCESGDAGRPAALNAD
                     TVMGQAFRQLAAAVQERLEKR"
     gene            complement(4605..5360)
                     /gene="trmB"
                     /locus_tag="PKY68_01865"
     CDS             complement(4605..5360)
                     /gene="trmB"
                     /locus_tag="PKY68_01865"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOH23818.1"
                     /translation="MAKNKLKKFEDLRNWSHVFEFPLKEGRRPECEFKGHWSASVFGN
                     DHPLVLELGCGKGEYTVGLGRLRPDMNFIGIDIKGARMWTGARQAKEEALGNVAFLRT
                     HIELLNSFFAPGEVAEIWLTFPDPQMKKVRKRLTSTYFMRMYQQVLAANAVLHLKTDS
                     RFMYEYSLAVLKANALPIHYQTQNLYSESLPVPDIQTFYEQQWLLRGISIKYLRWSIP
                     PVTNWKEPEVDIEPDEYRSFGRSARILPKDKQL"
     gene            complement(5376..6398)
                     /locus_tag="PKY68_01870"
     CDS             complement(5376..6398)
                     /locus_tag="PKY68_01870"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOH23819.1"
                     /translation="MNKNINWSELSFGYLPTDYNVRCYFRRGKWGELEVSSSENITMH
                     MAATCLHYGQEAFEGLKAFRGKDNKIRIFRLDENAKRLQSSAETILMEKFPLEMFREA
                     VLKVVKLNEAYCPPYESGASLYIRPLLIGTGPRVGVKPADEYLFVVFVSPVGPYFKEG
                     FKPTNMVIMREFDRAAPQGTGHVKVGGNYAASLHSGEIAHAKGYSAVLYLDSKEKKYI
                     DECGPANFFLIRGNSYVTPASHSILPSITNKSLMQLAEDMGMEVIRRQIRVEELNDFE
                     EAGACGTAAVISPVARIDDADTGKSYVFSKDGKPGKVCEKLYHKLRAIQYGDEPDTYG
                     WTTIVE"
     gene            complement(6443..8563)
                     /locus_tag="PKY68_01875"
     CDS             complement(6443..8563)
                     /locus_tag="PKY68_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOH23820.1"
                     /translation="MVLLFVNINGGLYAAGELSGREERKIRSIISKMSLEEKAGLLHG
                     NSKFYVGAIPRLGIPELALSDGPHGVRAEINRDNWSYAGWTNDSSTYFPTGTALAATW
                     NPDLACLRGEVLGEEARFRGKDILLGPGVNIIRSPLCGRNFEYMSEDPYLTAQMTTAY
                     IRGLQSRDVAACVKHYLANNQETDRGSVDVYMSDRALHEIYLPAFKAAVQEAGTYSIM
                     TAYNKFRGLWCAEHPYLLDSLMRQELGFDGIFVSDWNGTHSTLASAKAGLDLEMGTDK
                     PYSEYYFAQPLIDAVRKGELPESLVDRKVAGILSLMMKLKIFDPAKRFECSFNTPEHQ
                     QATYRSAAEAIVLLKNDKGILPLDATKIKSLAVIGDNATRKHSFGGQSSEIKALYEVT
                     PLEGLKNRAKGRFEISFAQGYEKSRRPSNRPDWALIDEATALAAQCDAAVVMAGLNHD
                     YDTESRDKPDMALPYGQLELIRAVVKANPNTVVVIIAGSAVDMAGFNYRVPGLLWAWY
                     NGMEGGNALADVLLGEVNPSGKMPFSLPFCLDQSNAHALGEFPGRNKKVDYFDDIFVG
                     YRWFDTYGIAPLYPFGHGLSYTQFEYSESRADKEEWSMNEKLRLSLVLSNTGRFDGAE
                     VVQLYVSDPESELPRPQKELKAFQKVFLKAGESRELVFELPISDLAYYNDIKREWQLE
                     PGTIKCLLGSSSRDIRREISIKVK"
     gene            8721..>11568
                     /locus_tag="PKY68_01880"
     CDS             8721..>11568
                     /locus_tag="PKY68_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOH23821.1"
                     /translation="MARINIYKASAGSGKTWRLSVEYIKLLIKNPESYRHILAVTFTN
                     KATTEMKERILHYLQLLSDPGTGSENPVLKTLEKETGLSRQTIAARAEQALSLLLHDY
                     SRFRVETIDSFFQSILRNLARELGLGSSMNIELEEDEILEEAVDLMIEKAGENDELLK
                     WLQDYIEENLLEGRDRKIAPALKKFGETIFSESFQSREKELYRVLSDKSFLNDYREEL
                     FKLKAQANEKLKGMGQRFFELIAQQGLGIDDLSNKNRGVAGYFLKLLNEEYKIGIFNA
                     TAQKALTDPEKWATAQHPRRTEIIKLAESTLIPFLQECEAERPKLYSVYHSCDLSLKH
                     IYKIGLLTDISLEVRAINREQNRFLLSDTAVLLKTLISDSDTSFVYEKAGTELKHIMI
                     DEFQDTSRVQWSNFKPLIFEGLSKNYDSLLVGDEKQAIYRWRNSDWRILGNLEKELAG
                     TELQFNTLSSNFRSEQRIVQFNNALFKQASLLSTASLENKLGIDCPDLALAYKEIEQQ
                     CSRKDEKGWVKAVFVRDDNNYYRHSLEQVVKQVEYLQEQGITPDQICILIRYKKHIPE
                     IAAYFAQYQTDHPGSPFCYTIVSDEAFRLDASPAVQILIAALKMLANPDDKLAREELE
                     QCRTRLLKTYGEEPDTFQGDLPAGFESQLNELIHIPLFELADLLIRLFDLGKIPAQES
                     YLFSFMDKLQEFLEKNTSDLSSFLRYWEDHLSSTTLPRASSMSGIRIMSIHKAKGLQF
                     HSLIVAFCDWPTEGNHRNLLWCHTDLQPFAALPLIPVNFQQLMNVTIFKEDYREECVQ
                     QFVDNLNLLYVAFTRAEKNLIICSKAPGPNKKDTEKNQEIKSVAELIYKILSDPAHPL
                     FSPHFKVENNEEDESILEGGEGRKKEETADTADDPVGNTQVAGFEYGKICTDSGEDEN
                     SRTEQDFAIEYRSFPRQTGFRQSNPSRDFVSGGVR"
BASE COUNT         3123 a         2997 c         2659 g         2789 t
ORIGIN      
        1 gtacttcctg ccgggtttcg gccaaatggg tgtgaataat cagggagcgt tcccgggcca
       61 tagccgccaa acgtttaaac aagtcttcac taaccgtata aacagcatgg ggagcaatgg
      121 taatctgcac tctcgatgaa gtttcgggta gattttcgag caagcgcaag ccccgttcta
      181 tgtattttgc accgttactt tccagaaaag taactccaac ggcagcccgg atgcccattt
      241 cttctacggc acgaatggtg tcgggaacat tgaaatacat atcgctaaaa aacaccgtac
      301 ccgatttgat catttccaga atagccagtc tggtgcccac atagtggtca tcaccctgca
      361 acttggcttc cagcggccaa atcttgtctc tcagccattc cgtcagatgc atatcttcgg
      421 cgcaacctct cagcaacgac atggcagcgt gggtatgcgc attgtagaag ggaggaagca
      481 aggccttgcc cttgccttcg acaacccgat tagccgataa atcgatcttg tcttcgatgc
      541 gcgtaaagcg attgccttcg atcaaaacat cctgagactt acgacccagc aatatatttt
      601 taattaaaat gctttccctg tattccgtgc tataatcaac agtcataact tgaagtttgg
      661 aagcgcaaat gtacaaaact tcgtcgccgt agtaaaatta tgtattttta cggactataa
      721 aacccgatat catgaaacgt tgcttcctat ttttacttct tgtgggtctg ctatggccta
      781 caaccccgtc ggcacaaaat aggtcctgcc ggctctttac tttcgatgcc gaccagatgt
      841 tcaataagta cctgctcagg caagtatatg aacaatatct ggaacgcgat gccttttttt
      901 gttccggatt cagcgctgct gaagcccgcg aatacggcaa taaagcaaaa caagcttatc
      961 tggacttact cggccctatg cctaatcgca cggccgtagc tgtggaacat tgcggcaacc
     1021 acaattttgg agcattcagc ttagagaaaa tcatctatca aagcagcccc aaccgcaggg
     1081 tcaccgccaa tctttatttg cccggtcaag ccggaaaaca ccctgtcgta ctgatgcttt
     1141 gcggacacgg agcccaaggc aaacaaacgc cgttttacca ataccaggca gctttgtatg
     1201 ccgccaacgg catagccacc atggtagtag accctcccgg ccagggagaa cgttttcagt
     1261 tgatcgacaa agacggcaac aacccagccc gtggtggcag cggcaccaac gaacacaccc
     1321 tcttgcttat gaattcactg atagtgggtg aatctatggt caaagacgaa ttatgggaca
     1381 acctgagagc catcgactac ctgtgcagcc ggccggatat cgatacgagc agaataggct
     1441 gtttcggatt ttcgggcggc ggcacccagg cggaatatct gcttgccttc gatacgcgta
     1501 tcaaggtggg aggcatcgga ggttatttta cgcaaagacg aagaagcatg gaagtgattg
     1561 cttcccagga cggttgtcag catttgcccg gcgaaggcct caaagctctt gaacaggtgg
     1621 actttgtaac ggcttttgcc cctaaaccac tgatcattat caacggagcc tacgattttg
     1681 ttgaccgtat aggagccgaa gcaggctaca gggaattaaa aaaattctat gctgcctgcg
     1741 gacggccgga acaggtactc ctatacaccg gcgacgacgg ccatgccttt ggagaagaaa
     1801 aacaaaggct ggtactggcc tggttcctca agtacctctg caacaaagag caaaagggcc
     1861 aaacttttcg caacccttcc tctaaagccg actatgcttc cgtaaaggct tttgcagagg
     1921 aaaccggcat aacacctgcc tacgactttg aacccatccc tgccaacgaa atgctctgca
     1981 cgcccaaagg aaatttaagc acctatctac cgaaaggaga actgctgaca ccggcacgca
     2041 atcaacaaat ctggcaagag caggccggat caagagccaa atttcacaca ctgagcccag
     2101 aagcacaaaa ccggcaagtc atctccttag ccgggataaa ccccggggtc gaggtcagag
     2161 tggaaaaagc cggatccacc gttttggaag gcataagatt tgaaaggtat attctgcacg
     2221 ccgaaaacga aacgccccta ccctgcctgt tacttttgcc cgataaaccg caacgggatg
     2281 ccgtcaggat tattctgcac gagcagggca aatgccttat agcagactcc tgcattcaac
     2341 tttgccaaaa cagcggtgag attatattac tggccgacct caggggcttg ggcgaaagcc
     2401 gcgataaacc cgaacgcaac gaaagcaaat atttcaatac ggaatacagt gtaggcatga
     2461 tgtcgctgca catcggcaag actttggtcg gacaacgtgc caccgatatt ttaatgctgg
     2521 cctcctttat cgggcaacac gaaacactca aaaattgccg cgacctgcag ttggaagcct
     2581 acggaaaatg cgttcctcct gccctgcacg ccgctttttt actggataac ctccgggaaa
     2641 tcaggctgca cagcgaactg ccttcgtggg agtatctatt ggaacatccc acaaccaaag
     2701 agcagctctc ctacattgta cccaaagctt caaaatacta cgacctgccc gatctgcaga
     2761 aggctctgat agcccgcaag atatcggtta tatactgaac ttaaggcttt aaattccatc
     2821 tcaatatgga tattgaaaaa aaacgcttca agctttcttt ttatgttccc actctgctcg
     2881 tgttcctgct cgccctggta caggccctgc aaagcggttt gcaggaaagc tggtacaggt
     2941 taggcatcta tcctcgcgaa atctccagtc ttccgggaat aatcacacag gtatttatcc
     3001 acgaaaactg ggcacatttt tttaataatg ccgttcccct ctttgttttg agttggtgtt
     3061 tttactgttt ctaccgggaa ctcttttgga aaggcagcct tttgctatgg ctcagctccg
     3121 gactgttcac ctggttgatc ggtagagcca actggcatat cggagccagc ggtttaattt
     3181 atgcctactc cttttttttg ttcttcagcg gattgatacg cgaacacaaa ccactgatgg
     3241 ccatatcgtt tgtagtggta ttcctctacg gtagcacctt ttggaatatg ttcccctggg
     3301 gagaattcaa cagagacatc tcctgggaag gtcatctgtc gggagccatg gccggcctgc
     3361 tttgggcctt gctgctaaga aagcaggggc ctcaggctcc acctcccccg gaagaggaag
     3421 aagacgaaga agaagcgata aattccgaat tggactctac ggcagaagaa cttgccgaaa
     3481 agaatccgga caaggacgaa gtcaaagtta ccggtgaaaa ttcaagccgg caaactgcca
     3541 tcaacgtttt tccaaacgct cctgaacagc agcagccaac tgcctgaaag cctgccccat
     3601 tacagtatcg gcgttgaggg ccgccggtct tcccgcatcg cccgattcac agacgctttg
     3661 cactaggggt atctgtccca gcagcggaac tcctctttct ttggccaact ctgctccccc
     3721 gtctttacca aacaaataat agcggttttg aggcagttcg gccggagtaa accaagacat
     3781 attttcgacc aaacccagga tgggtacatt gatcttttcg ttttggaaca tatcgaccgc
     3841 cttacgggca tcggtcagtg ccacctgttg aggggtggta accacaatgg ctcccgaaag
     3901 tttcaaattc tgtaccaaag taagatgaat atcgctggta cccgggggca agtctatgag
     3961 gaaataatcc aattcacccc aataggtatc tcctatcaat tgtttcaggg cattggaggc
     4021 catagggcca cgccagacga tggcattctc tttttgtaca aaaaatccta tagataacac
     4081 cttgagcccg tatttttctt cgggaatgat tcggtcgcgt cccaaaatat tctccatgac
     4141 cggcctgact tcttccatat ccaacatctt gggaatggaa ggaccaaaaa tatccgcatc
     4201 cagtaaaccc actctatagc cttcggcagc caaagccacc gccagattgg tggtgatagt
     4261 ggatttaccc acgccaccct tgcccgaagc aataacaaca acgtttttca ccccgggcaa
     4321 cagggcttct tcttccttga cgggagcagc cgggcggacc acaaccttga tattcccttt
     4381 tatatcgacc tcttctccta cataagtcag gatggccgtt tcggcagctt tcactaccga
     4441 cttgataaag ggatcgtttt gtttttcaaa aataagtgaa aaactcacct tattaccttc
     4501 gatgcgaatg ttgtcttcta ccataccgga agaaaccagg tcttgggaag ttccggggta
     4561 acgcactttg gacagggcgt ccaaaatcaa tttaggataa agattcatag ctgtttgtct
     4621 tttggtaata tgcgggcact tcgtccgaaa cttctgtact cgtccggctc aatgtcaacc
     4681 tcgggctctt tccagttcgt aaccggcgga atggaccacc ggaggtactt gatgctgatg
     4741 ccgcgcaaca accattgttg ttcgtaaaat gtctgtatat cgggaaccgg gagcgactcg
     4801 gaataaagat tctgagtctg gtaatgaatt ggcaaagcat tggcctttaa gacggcaagg
     4861 ctgtactcgt acataaaacg actgtcggtt ttgaggtgca agacagcgtt tgctgccaaa
     4921 acctgctggt acatacgcat gaaataggtt gaagtcaatc gcttgcgtac tttcttcatt
     4981 tgcggatcgg gaaaagtcaa ccaaatttcg gccacttcgc cgggggcaaa aaaactattc
     5041 aatagctcaa tatgagtacg taaaaaagcc acgttcccaa gggcctcttc cttagcctga
     5101 cgggcccctg tccacatgcg ggcccccttg atgtctatac ctataaaatt catgtccggc
     5161 cgcaaacggc ccagccccac cgtgtactct cctttgccgc aacccagctc taaaaccagc
     5221 ggatgatcgt tgccaaaaac acttgcactc caatggcctt tgaactcaca ctcaggccgc
     5281 cggccctctt tgagcggaaa ctcaaacaca tggctccagt tgcgcaaatc ctcaaatttt
     5341 ttcagtttgt tttttgccat agaaggggta cagcctcact cgacaatagt agtccagccg
     5401 taggtatcgg gctcatcccc gtattgaata gccctgagtt tatggtataa tttttcacag
     5461 actttgcccg gtttgccgtc tttggaaaac acatagcttt ttccggtatc cgcatcgtct
     5521 atccttgcta ccggactgat tacagccgcg gtgccgcaag cgcctgcttc ttcgaaatcg
     5581 ttgagttctt ctacccggat ttgcctgcgt atcacttcca tgcccatatc ttcggccagt
     5641 tgcataaggc ttttgttggt aatggagggc aggatggaat gcgaagccgg ggttacatag
     5701 ctgttgcccc tgattaaaaa gaaattggcc gggccgcatt cgtcaatgta tttcttttct
     5761 ttcgaatcga gatacaacac agccgaataa ccttttgcgt gagctatttc gccggaatgc
     5821 aaactggcgg cgtagttacc gccgaccttt acatgtccgg taccttgagg agcagcacgg
     5881 tcaaattccc gcataattac catattggtc ggtttaaagc cctctttgaa atagggaccc
     5941 acaggcgata caaataccac aaacaaatac tcatcggccg gcttaactcc cactctcggg
     6001 ccggtaccga tcaacaaagg ccttatatac agagaggctc ccgattcgta gggcggacaa
     6061 taggcttcgt tcaattttac cactttcagc acggcctctc tgaacatttc gaggggaaat
     6121 ttttccatta aaatggtttc ggccgaagat tgcagacgct tggcattttc gtccaaacgg
     6181 aaaatccgta ttttgttgtc tttgccccta aaggctttta aaccttcgaa agcttcctga
     6241 ccgtaatgca aacaggtagc agccatgtgc atagtaatat tttcagagga cgaaacttcc
     6301 agttctcccc acttgcccct gcgaaaatag cagcgaacat tgtagtcggt aggcagatag
     6361 ccaaacgaca attcagacca attgatgttt ttgttcatag cagttatata tcttgcgctt
     6421 tattaatatg atattccagg tcttatttga ccttaatact tatttcccgt cggatgtctc
     6481 tcgacgaaga gcccagcaaa cacttaatcg tacccggttc cagctgccat tcccgtttaa
     6541 tgtcgttata gtaggccaaa tcgcttatag gcaactcaaa aaccaattca cggctttcgc
     6601 cggctttcag gaaaactttt tgaaaagcct taagctcctt ttggggtcgg ggcaattccg
     6661 attccgggtc ggaaacgtac aattgaacca cttcggctcc gtcgaagcgg ccggtattgc
     6721 tgaggaccaa actgagccga agtttttcgt tcatactcca ttcctcttta tccgcacggg
     6781 attccgagta ttcaaattga gtataagaca agccgtgacc aaaaggatac aaaggagcaa
     6841 tgccgtaggt atcgaaccag cggtaaccca caaaaatatc gtcgaaataa tccactttct
     6901 tgttgcggcc ggggaattca cccaaagcat gggcgttgga ctgatccagg caaaacggca
     6961 aactaaaggg catcttgccc gaggggttga cctctcccag cagaacgtcg gccagggcat
     7021 taccgccttc cattccgttg taccaggccc acagcagacc gggaacccgg taattgaaac
     7081 cggccatatc gaccgccgag ccggcaataa tcaccacaac ggtattggga ttggctttga
     7141 ccacggccct gattaactcc aattgaccgt atggcaatgc catatcgggc ttgtcgcgcg
     7201 actcggtatc gtagtcgtga ttgaggccgg ccattacaac agccgcatcg cattgcgccg
     7261 ccagggcggt tgcctcgtca attaaggccc aatcgggtct gttcgaagga cgccggctct
     7321 tttcgtaacc ttgggcaaaa gaaatctcaa accggccttt ggccctgttt ttcaagcctt
     7381 ccaaaggagt cacttcgtac aaggctttga tttcggaact ctgcccaccg aaagaatgtt
     7441 tacgggtggc gttatcacca atcacggcca gggattttat tttagtcgca tcgagaggta
     7501 aaataccctt atcgttctta agcaacacga tggcttcagc cgccgagcga taagtagcct
     7561 gctggtgttc gggggtattg aaactacatt cgaagcgttt ggcaggatcg aaaattttga
     7621 gtttcatcat caggctcaaa atcccggcca ctttccggtc gaccagggat tcgggcagct
     7681 cacctttgcg aacggcatcg atcagaggtt gcgcaaagta gtattcgcta tacggtttat
     7741 cggtgcccat ctccaggtcc aaacccgctt tggcagaagc caaagtagaa tgggtgccgt
     7801 tccagtcgct gacaaaaatc ccgtcgaaac caagctcctg tcgcataaga ctgtcgagca
     7861 aataaggatg ctcggcacac cagagtcccc gaaacttatt gtaagccgtc atgatggaat
     7921 aggtgcccgc ctcctgcaca gcggctttaa aggccggtaa atatatctca tgcaaagccc
     7981 ggtcgctcat gtacacatct accgagcctc tgtcggtctc ctggttgttg gccaggtaat
     8041 gcttgacgca ggctgccaca tcgcggctct gcaaacccct gatgtaggcc gttgtcattt
     8101 gtgctgtcag gtaaggatct tcgctcatgt attcgaagtt tctgccgcaa agcggagaac
     8161 gaataatgtt cactcccgga cccagtaaaa tatctttgcc cctgaaacgg gcctcttcgc
     8221 ccagaacttc gccgcgcaaa caggccagat cgggattcca ggtggcagca agcgcggttc
     8281 cggtggggaa ataagtagag gaatcgttgg tccagcctgc gtaactccaa ttgtcgcgat
     8341 tgatttccgc tcttactccg tgaggaccgt ccgaaagggc cagttcggga atacccagcc
     8401 gggggatggc cccaacgtaa aatttagaat taccgtgcaa caagccggct ttttcttcga
     8461 ggctcatttt ggagataata gagcgaatct ttctttcttc ccgtcccgac agctcgccgg
     8521 cggcataaag tccgccgttg atgtttacga acagcagaac caaacataaa tggtaaaatc
     8581 cggctttcat ggatgcagaa tgaatcaatt attatccttg tagaacgaga gacaaaggta
     8641 agaattttct atctttggca cataagaatt tttctccgat tttctgtctc tcaggggaaa
     8701 ttagcattga aaacagcaaa atggcccgga ttaatattta caaagcctct gccggctcgg
     8761 gaaaaacctg gcgtctgtcg gtggaataca tcaaactgtt gattaaaaat cccgaatcct
     8821 accggcatat cttagcggtg acttttacca acaaagccac caccgaaatg aaagagcgta
     8881 ttctccatta cctgcagctg ctttcagacc ccggcaccgg aagcgagaat ccggtgctga
     8941 agaccctcga aaaagagacc ggcctgtccc ggcagaccat tgccgcccgg gcagaacaag
     9001 ccttaagcct tttgttacac gattacagcc gcttcagggt agaaaccatc gacagttttt
     9061 ttcagagcat cctgcgcaac ctggccaggg aactgggctt gggcagctct atgaacattg
     9121 aattggaaga agacgaaatc ctggaagagg ccgtggacct gatgatagaa aaagcggggg
     9181 agaacgatga actactcaaa tggttacaag attacatcga agaaaatctg ctcgaaggac
     9241 gcgatcgcaa aatagctccc gccctcaaaa aattcggcga aactatattt tcggaatctt
     9301 ttcagtccag agaaaaggaa ctctatcggg tgctctccga taaatccttt ctaaacgatt
     9361 atcgcgagga actctttaaa ctcaaagccc aggccaacga aaagctgaaa ggaatggggc
     9421 agcgtttctt tgagcttatc gcgcaacagg ggcttggaat agatgattta tccaataaaa
     9481 acaggggggt agccggctat tttttgaaac tgctcaacga agaatacaaa ataggtattt
     9541 ttaatgccac cgctcaaaaa gcccttacag atcccgaaaa atgggccaca gcccaacacc
     9601 cccggcgaac cgaaataata aagctggccg aaagtacgct gataccattt ctgcaagaat
     9661 gcgaggctga gcggcccaaa ctctattccg tctatcattc ctgtgacctc agccttaaac
     9721 acatctacaa aatcggcctg ctcaccgaca tttccctcga agtcagggcc atcaaccggg
     9781 aacaaaaccg ttttctgctt tcagacacgg ccgtcttgct gaaaaccctc ataagcgaca
     9841 gcgacacttc ctttgtttac gaaaaagcgg gcacggagct gaaacacatc atgatagatg
     9901 aatttcagga cacttcgcgc gtccaatggt ccaacttcaa gcccctgatt tttgaaggcc
     9961 tttccaaaaa ttacgacagc ctgctggtgg gggacgaaaa acaagccatt tatcgctggc
    10021 gcaacagcga ctggcgaatt ctgggtaatt tagaaaagga actggccgga acggaactgc
    10081 aattcaacac acttagcagt aattttcgca gcgaacaacg catagttcaa ttcaacaatg
    10141 ctctttttaa acaggccagc ctgctctcca cagcttcgtt ggaaaacaaa ctcggaatag
    10201 actgcccaga tcttgccctg gcctacaaag aaatagagca acaatgctcc cggaaagacg
    10261 aaaaaggctg ggtaaaagcg gtctttgtgc gagacgacaa caattattac cggcacagcc
    10321 tcgagcaagt ggtcaagcaa gtagaatatc ttcaggaaca gggaattaca ccggatcaaa
    10381 tttgtatatt gatacgttat aaaaaacaca tacccgaaat tgccgcctac tttgcgcagt
    10441 atcagacgga tcatcccggc agtccctttt gctataccat agtatcggac gaggcttttc
    10501 gcctggacgc ctccccggcc gtacaaatac tgattgccgc tctcaaaatg ttggctaatc
    10561 ccgacgacaa gctggccagg gaagaactcg aacaatgccg gacaaggctt ttaaaaactt
    10621 acggcgaaga gccggacacc tttcagggag acttacccgc aggcttcgaa tcacagttga
    10681 acgaattgat tcacattccc ttgtttgaac tggccgacct gctgatacgc ctttttgatc
    10741 tcggcaaaat accggctcag gaaagttatc ttttcagctt tatggataag ctgcaggaat
    10801 ttctcgaaaa aaacacctcc gacctgagca gcttcctgcg ttattgggaa gatcatctca
    10861 gtagcaccac cttaccccgg gcctcgagta tgagcggaat tcgcatcatg agcatccaca
    10921 aagccaaagg tctgcaattc cacagcctta ttgtcgcctt ctgcgattgg cccaccgaag
    10981 gaaatcaccg taacctgctt tggtgtcata cagatctgca gcctttcgcc gccttgcctt
    11041 tgattccggt aaatttccaa caacttatga atgtgaccat attcaaggaa gattaccggg
    11101 aagaatgcgt acaacaattc gttgataacc tcaacttgct gtacgtggct tttacacggg
    11161 ccgaaaaaaa tttaatcatc tgcagcaaag cccccgggcc caacaaaaag gacacggaaa
    11221 agaatcagga gataaaatcg gtggccgaat taatttataa aatactgtcc gacccggctc
    11281 atcccctgtt ttcgccgcac tttaaagtcg aaaacaatga ggaggatgaa tcgattttgg
    11341 aaggaggaga aggaagaaaa aaagaagaaa ccgcggatac agcagacgat ccggtgggaa
    11401 acacccaagt cgccggtttt gaatacggca agatctgtac cgattcaggc gaggacgaga
    11461 attcaaggac tgaacaggac tttgcaatcg aatacagaag ttttccccgc caaaccggat
    11521 tccggcaatc caatccttcg agagattttg tgtcgggagg cgtcaggg
//
LOCUS       DAOSGL010000029        11547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4700_length_11547_cov_4.042116, whole genome shotgun sequence.
ACCESSION   DAOSGL010000029 DAOSGL010000000
VERSION     DAOSGL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4700_length_11547_cov_4.042116"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2816
                     /locus_tag="PKY68_01885"
     CDS             <1..2816
                     /locus_tag="PKY68_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH23822.1"
                     /translation="VLMPLGTVNNSHLSAAAVFLDQDKSTEVAIMPEQLLQAKVSGLN
                     AMFRSGMEGSGANLFVRGFNSLYASNQPLLVIDGMVLENAQFGNSLIEGYISTPLGAI
                     DVQDIDRITLIKDASSVYGVKGANGALLIQTKRTTDLATKINLMAVQGLSLQPSKIPM
                     LNAKDARHYLIDMYQSTGQYSATEIQALPFVDQQIPVQQPWGYSGNVDYYRYNKNTDW
                     QDEIFRPAYKQHYSLNVSGGDEVAVYGLSLGFVNKESVVEGSDFNRFNARINTGIKFN
                     PAIQVFTNMSFVYGMKNLANEGNAGYLNPIYSALVKAPFTTSFVYDQHNRRSPKHEEV
                     DFFGHANPAVVTGLTDTENSFYRFLGNLDLNIKLNDKLKLVSIFGIDYNKERERIFYP
                     TGGIPYESIPLAAVSNQQQHRVERLFSIFSDTRLHYARNFGYQRRLDASLGLRYLNSR
                     AEDDYGKGYNSSSNYFVSIGSGSNRLYQTGGAIGNWNWLALYGNVDYNHKNRYFVNAL
                     LSYDASSRYGSEIAAFQAFPSLGAAWLISSEEFMKNSQWIDMFKLRANYSMTGNDGIG
                     NYAAKRYYVTQNFLGNYGLVRGNLVNEKLMPERNNKLSAGLDLALLNERLNISVDLYH
                     STVKDMLIHAEAPAYTGFRFYIENGGEMRNRGIDASLNARLVERGAFSWDLGLAASHY
                     QNEILSLSTGDFNTPIAGGLVRTEVGKPLGIFYGYKTEGIYQTAAEAAENQLYKMSGT
                     VKVPYAAGDVRYVNVNGDDLIDQNDMQQIGNPNPDLHGSLWTDLAWKGLSLSAVFRYS
                     LGNDVYNYTRRNLESMSSFANQTQAVLNRWKSEAQTATMPRAVYGDPMENSAFSDRWI
                     EDGSYLKLKTLSLAYAIPIQSGVLRDITVYGTAENLLCLTAYKGYDPEIISASAEMPL
                     YYGVDAFTMPTCRTFYLGIKIGL"
     gene            2836..4575
                     /locus_tag="PKY68_01890"
     CDS             2836..4575
                     /locus_tag="PKY68_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH23823.1"
                     /translation="MKTNKYIALVVLIGSLVLSSCVQTDIDDALEYDKHYKTTEDADN
                     AILGVYSSFMRMADQMLILNELRGELTDLTAYSGTELQEIDAANPSASNTLADPTPYY
                     NVILNCNDALENFKIMLQNNRMTQEEFRERYADLTAMRCYMYLQLAAQFGRVYYITKP
                     IVNLADMRAYSDSAKVTIDQLLPLLIADMESLEFMDPYVTSPLVQYNLDGYDLSYFFI
                     NKKLMLADLYLWNGDYLKAAFLYKEIMDTYSDGEATVNCYTYKLAAATTWTTATAYST
                     WAYQIFFLRYKEDDMEAYNNQWVNIFSDDMTGRRIRYEWIWSMSYDAAYAPSFPLIDL
                     FAKTEDGGSYLLKPSAYAIEDLYGSQKMQNGFTLDGRGPEASFYQSPDGDYVISKYLY
                     RYDPSKPYERAGRLYLYRGGLLHLRYAEAANRAGYPDVAYAIVNQGIAGTYGVGLESV
                     VDYPEPFYFDPRYTTTPYYRGPWRYNFGIRGRAWLQSKEGSVFADKTLAECVNKQDSI
                     RVMEKIIMDECALECAYEGHRFPDLVRVARRMNREAAGSGNNYLQAVLQGKYLKSGRA
                     VPDYSSEEKWFLP"
     gene            4628..6376
                     /locus_tag="PKY68_01895"
     CDS             4628..6376
                     /locus_tag="PKY68_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23824.1"
                     /translation="MKKIYYLTFAFLFCLISVGAYSQALIETDYVVEFPFDESDYAGE
                     TQVIISDATVTFTADAFNTCGLPFGTGTLITMEEGAVIIMYDSPETWFPDADAEENAD
                     YIDYLTDSEGQKVTSVPFNFLINGNSRIVLDSKCRFGGTITGSGNLTLVVGDSTILDS
                     DLGISSLGEFTGTLTIEVKEGNNQLLIGSTYPGTCPIDNVTYNNASARCYHSIPWTLV
                     LPDGIHVYTHDKTHTCFPRTIGHAYVHCSSTCTFRSNQLHLYDLEIDGGADNRNFEMY
                     SVGQGFNYPVNYLGAYAYVRTGALYVNGDKASFSNIINRISIRGREMFVGGVGFIDCT
                     VEGKAGTTAHIKPGATGENDIGTLTILKELYLNSNNSVDIDFGPGGKSDKLILVGGGA
                     FSGEFTRLWVNIMDDFYDNPVAGDYKVLDAPTLRMNEVPVLDTVFTGTFTTTIDSIYS
                     GDTLIRVDTIVTADIERIDTIGWEISNNLVVNANQLQTGQHKDSLPEGYAYNFDRFFS
                     EGIIAITGPGYEEGNIVNGPSGINQVKLPDKFVKQREIFNLTGVRTKTLTRGINIIRT
                     TFSDGSVEVQKYFYDE"
     gene            6420..>11547
                     /locus_tag="PKY68_01900"
     CDS             6420..>11547
                     /locus_tag="PKY68_01900"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23825.1"
                     /translation="MKKFCVLLLALMFLPTWLGAQTVFDNTTVNSTYVLSDTVLPTVA
                     NVDTAEAFSTTQFIMGPGLRVKTFKKVSSDLTATLFEEPNATALTIGGRLPDYVLTFI
                     LRPSKGVTFKPTNISFKAFKNGTGDQVKIDVMNQRGEAAETLLSSIDIARNNEAEGYY
                     TTVSLDITDGVASNDTYILKLYPWGKGMRAGKGCGIGDLVITGVVHGTVIPVPKYTIT
                     PLVSPEGAGEISINPPGTLFDEGTELNLIALRNFGFEFSNWKSSETLLTDSSVLELVL
                     MSDTTVTAHFTPVSTYELAINAPAGVKDYMFDLDPPFFPVAGKKMYETGTSVLVSPRS
                     NAIMDFTGWEGGSAGPDTTVNMTANVTLQANYDVKTYLAGWDFYVAGNKDRFADFASA
                     ENVVTKLMLINADGTSTSWLDKSEMLGASYEGKYCAVNWKPLADKYYYQTCVNASMYN
                     NIKVVASMLYNYNAYQKQLVEYSLDGTNFTTLDTIVMSTNKVWYTKTVALPQAADYAS
                     KVYIRWIPDYTSDIVGTSSTNDGTSITDIFVYAEGLKTDAKADITDTTVVNPNFDTNI
                     NGWTSTTGASSNKIATNKTGDFTGPFWENWRNTSFTGKMSQIITGLPNGSYLLRAAAF
                     CNNGGDGFYLFANSGKTQVQTTEPTFHEVVGVVTDGKLEIGLLAELPTNNWVGLDNVK
                     LYSLGFILNDMITALEELIVEAEAITGDMQASVATGLADAISNAEAAVASRSEVHVVA
                     SLTALRAAIDAAYSSIALYAAFPALQTKANYIYSATAAEAEALQAAITTSNDTYVAKT
                     ANQTTYDNLVAAMVSYRAANDVTAYVVNPDMTEGKTVGWASDFGEQTAAYPGFEGRFL
                     EKWVSAANNLADFNLSQIISDLPNGTYTLSAWCIATKQNQAGEAAKTYVKNVYLYANN
                     DSTPVSSLNGVPEEFMINVTVADNTLKLGFTGINCTANWIAIDKVRLMSHDVSTLLAA
                     KQVSLNALIGEAEAVVAGVDSAMLGAESFKYAPAKLDALNAAIDSAKVFAGASVNEYV
                     AMENFLTAAMNNLRQMNQPLATQFFYIVHSSLNHLDVADGVKIKTAADTAYYQWFKLL
                     PIGSGELNAYHLKTLDEKFVGLTGTNNWDMSAQDVASSDNNVKFIIEMIDGSKYKLRT
                     IKGLLGTDAVTSGSGVYGNKAAGANSEWSLAEVGEAVYQYHNSSVELKTVLDEANAQY
                     DSTLIAADEFAAAIAAAQAVYDKGTGASVAELQAAVVALNEAIAAFEEANEVPPIVIN
                     KDDYVAFYSQDYEFVAQASDVWTSPNNQAGFSLVEEAGNKYINYDHGNVNSRGAYSNF
                     DIDYAGISEYLVEFDLALTAGNNQATEFALKGTDFAYAGGNVNWGAEKGYLFKLSTTN
                     STTWTIVGSGAESYTVELNALEFYHFLALVRADGIQLTITDAADSVVLDNVAITLGSE
                     GIECVGLHYQAGRYQSKMKIDNILLSKPVPLTKVTWYMQDYENVASAADVWTSPNNQA
                     GYSLVEEEGNKYIYYNHGNVNSRGSYSNFEIDFADVDKYILEFDLALKAGNNQATEFA
                     VKGTDFAYTGDNVNWGAGGGYLFKLFTTNSTTWTIAGSGAESHTVELNSLEFYHYSAE
                     ISAEGILLTITNAADSAVLDKAAITSGSAGIECVGLYYQAGRYQSEMKIDNIAFSKLV
                     KGVVMVEPTVLMTGVSGISRIITMLSTQPTAILKYVLVSAE"
BASE COUNT         3024 a         2686 c         2800 g         3037 t
ORIGIN      
        1 aggtgctgat gcccttgggg acggtaaata acagccacct gagcgcagca gccgtttttt
       61 tggatcagga caaaagcacc gaggtggcta ttatgcccga acagctttta caggctaagg
      121 tatcgggttt aaacgccatg ttcagatcgg gtatggaagg ctcgggagcg aatctttttg
      181 tccggggttt caattccctg tatgcttcca accaacctct gctcgttatc gacggcatgg
      241 tgctcgaaaa tgcgcaattc ggcaactccc ttattgaagg atatatttct acgcctctgg
      301 gggctattga cgtgcaggat atcgaccgca tcactctgat aaaggatgcc agctcggttt
      361 acggagtgaa gggggccaac ggtgcgctgt tgattcagac caaacgcact accgatttgg
      421 ccaccaaaat caatctgatg gcggtgcagg gattgagttt gcagccttcc aaaattccca
      481 tgctgaacgc aaaagatgcc aggcattatc tgatcgatat gtatcaaagc accggccaat
      541 actctgcgac agagatacaa gccctgcctt ttgtcgatca gcaaattccg gtacagcagc
      601 cctggggcta ttcgggcaat gtggattatt acaggtacaa caaaaacacc gactggcagg
      661 atgagatttt caggccggcc tataagcagc attattcgct caatgtttcc ggaggtgatg
      721 aggtagccgt ctatggtttg tctctgggct ttgtgaataa ggaatcggtg gtagagggtt
      781 cggatttcaa tcgttttaat gcccgtatca ataccggaat taagttcaat ccggccatac
      841 aggtttttac caacatgagc tttgtgtatg gaatgaaaaa cctcgccaac gagggtaacg
      901 ccgggtatct gaatccgatt tattcggcac tggtgaaggc tccctttacg acaagttttg
      961 tgtatgatca gcacaatcgc cgttcgccca aacacgaaga agtggatttc ttcggccatg
     1021 ctaatcctgc cgttgttaca ggtctcaccg ataccgaaaa ttcgttctac cgttttttgg
     1081 gcaacctcga cctgaatatt aagctgaacg ataagcttaa actggtctcc atttttggta
     1141 ttgattacaa caaggaaagg gaacgtattt tctatccgac gggtggtatt ccttacgaaa
     1201 gtataccgct ggcagccgtc agcaatcagc aacaacacag ggtggaacgt ctgtttagta
     1261 ttttcagcga tacccgcttg cattacgcac ggaactttgg ttaccaacgg cggctggatg
     1321 cctctctggg cttgcgttat ctgaattccc gtgccgaaga cgattacggc aaaggttaca
     1381 actcttcgag caattatttt gtatccatag gttccggctc taacagactg taccagaccg
     1441 gaggagctat aggaaactgg aattggctgg ccctgtatgg caacgtcgat tacaaccaca
     1501 agaatagata ttttgtcaac gccttgcttt cctacgatgc ttcttcgcgt tacggatcgg
     1561 aaattgccgc ttttcaggct ttcccctccc ttggagcagc ctggttgatt tcttcggaag
     1621 agtttatgaa gaacagccaa tggatagaca tgttcaaact tagagccaat tacagtatga
     1681 ccggcaacga tggaataggg aactatgccg ctaagcgcta ttacgtgact caaaatttcc
     1741 tgggcaatta cggtttggta agggggaatc tggtaaacga gaaactcatg cccgagagga
     1801 ataataagct gagtgccggc ctggatctgg ccctgctgaa cgaaagattg aatataagtg
     1861 tggatttgta tcacagcact gtcaaagata tgctcataca tgccgaagct ccggcttata
     1921 ccggttttcg tttttatata gagaacgggg gtgaaatgcg aaatcgtggt atcgatgcat
     1981 cgctaaacgc ccgactggtc gaaaggggag catttagctg ggatctgggc ctggcagcct
     2041 ctcattacca aaacgagatt ttgtcgctaa gcaccggtga tttcaacacc cctatagccg
     2101 gaggcctggt caggacagag gttggtaagc cattgggtat attctacggt tacaaaacgg
     2161 agggcattta ccaaacagcc gccgaagctg ccgaaaacca actctataaa atgtcgggga
     2221 cggtaaaagt gccttatgca gccggcgatg tgcgctatgt caatgtcaat ggtgatgatt
     2281 tgatcgatca gaacgacatg cagcagatag gcaatcccaa tcccgatttg cacggctctc
     2341 tctggaccga tcttgcctgg aaaggcctga gtctctcggc cgtcttccgc tattctctcg
     2401 gtaacgatgt ctataattat acccgcagaa atctggagtc gatgtcttcc tttgccaatc
     2461 agactcaggc tgtactcaac cgctggaaat cggaggctca aacagccact atgcccaggg
     2521 cagtttatgg agatcccatg gagaattccg ctttttcgga tcgttggata gaggacggtt
     2581 cttacctgaa gctcaaaacc ctgagcctgg cctatgctat tcctattcaa agcggtgttc
     2641 taagagatat caccgtgtac ggtaccgccg aaaacctact ttgcctgaca gcttacaaag
     2701 gctatgatcc cgaaatcatc agcgcttcgg ccgaaatgcc gctttattac ggtgtggatg
     2761 cttttactat gcccacctgc cgaacttttt atttaggtat taaaattgga ctataagcat
     2821 aagaattcta ccattatgaa gacaaacaag tatatagcct tagttgtttt gataggcagc
     2881 ctggtcttgt cctcctgcgt gcagacggac atcgacgatg cgctggaata cgacaagcat
     2941 tacaagacaa ccgaagatgc cgataatgcc attttgggag tgtattcgtc ttttatgcgt
     3001 atggccgatc agatgttaat tctgaacgaa ctccgcggag agctcaccga tttgaccgcc
     3061 tattcgggca cggaattgca ggaaatcgat gccgccaatc cttccgcatc gaataccctg
     3121 gccgatccta ctccctatta caatgtgatt ttgaattgca acgatgctct cgagaatttt
     3181 aaaataatgc tccaaaacaa ccggatgacc caggaagaat ttcgtgagcg ttacgccgac
     3241 ctgacggcga tgcgttgcta tatgtatttg cagttggcag cccagttcgg ccgggtgtat
     3301 tatattacaa aacccattgt taatctggca gatatgcggg cgtattccga ttcggctaaa
     3361 gtcactatcg atcaactgct gcccctgttg attgcggata tggaaagcct ggaatttatg
     3421 gatccctacg tcacttcgcc tttggtgcaa tacaatctgg acggttacga cttgtcgtat
     3481 tttttcatca acaaaaagct catgttggcc gacctctatc tttggaacgg cgattatctt
     3541 aaagccgcct ttttatataa agaaataatg gatacctaca gcgacggaga ggctacggta
     3601 aactgctata cctataagct ggcggcggct accacctgga ctacggccac ggcttattcc
     3661 acctgggctt atcagatttt tttcctcagg tacaaagagg acgatatgga agcctacaac
     3721 aatcagtggg tgaatatctt ctcggacgat atgacgggcc gcaggattcg ttacgaatgg
     3781 atttggagca tgagttacga tgcggcttat gccccctctt ttcctctgat tgacttgttt
     3841 gccaaaaccg aagacggagg tagttatttg cttaagcctt cggcttacgc gatcgaagat
     3901 ttgtatggca gtcaaaagat gcaaaacggc tttacactgg acggacgcgg gcctgaagct
     3961 tccttttacc aaagccccga cggggattac gtcatcagta aatacctgta tcgctacgat
     4021 ccctccaaac cttacgaacg agccggtagg ctttatctct accgcggagg tttgctccac
     4081 ctgcgttacg cggaagccgc caaccgggcc ggctatccgg atgtggctta cgctattgtt
     4141 aatcaaggta ttgccgggac ctatggggtt ggcttggagt cggtggtaga ttacccggaa
     4201 cccttttact ttgatcctcg ttatactact acgccttatt ataggggtcc ctggaggtat
     4261 aatttcggca tcaggggacg cgcctggctg caatccaaag agggctctgt ttttgccgat
     4321 aagacgttgg ccgaatgtgt caacaaacaa gactccatcc gggtgatgga aaaaataatt
     4381 atggacgaat gtgctctgga atgtgcctac gagggtcatc gttttccaga ccttgtacgt
     4441 gtggcccggc gcatgaaccg cgaagctgcg ggtagtggta acaactattt gcaggctgta
     4501 ttgcaaggga aatacctaaa atcgggacgg gctgttcccg attattcaag cgaagaaaaa
     4561 tggtttcttc cttaattaaa caactaacga actaagtatt aacaatcaca cctaaaacca
     4621 attgactatg aaaaaaatct actacttaac ttttgctttt ctattctgcc tgatttcggt
     4681 cggagcttat tctcaggctt tgatcgaaac agattatgtg gtggaatttc cttttgacga
     4741 atcggattat gccggtgaaa ctcaggttat tatttccgac gcgactgtaa cttttactgc
     4801 agacgctttt aacacctgcg gtttgccttt tggcaccggt acgctgatta ctatggaaga
     4861 gggcgctgta ataattatgt atgattctcc cgaaacctgg tttcccgatg ccgatgccga
     4921 agaaaacgcg gattacatcg attatttaac cgattcggag ggacaaaaag tgacatccgt
     4981 accctttaat ttcctgatta acggaaacag ccgcattgtg ctcgacagta aatgccgctt
     5041 tggaggcact attacaggca gcggtaatct gacactggtg gtcggcgact ctaccatcct
     5101 ggattccgac ttgggcattt caagtttggg tgaatttacc ggtacactca ccatcgaagt
     5161 caaagagggc aataatcagc ttttgatcgg ctcaacttat ccgggtactt gccctataga
     5221 caatgttacg tacaataatg catctgccag gtgttaccat tccatcccct ggactttggt
     5281 gttgcccgat ggtatccacg tttatacgca cgataaaacg catacttgct tcccgagaac
     5341 catagggcat gcttatgtgc attgcagttc aacctgtact ttccgttcca atcaacttca
     5401 tctctacgat ttggagattg acggaggagc cgataaccgc aactttgaaa tgtattccgt
     5461 gggacaggga tttaattacc cggtgaatta cctgggcgct tatgcttacg tgcgtaccgg
     5521 agctttgtac gtgaacggag acaaggcctc tttttcgaac attatcaatc gcatttccat
     5581 tcgtggtcgt gaaatgtttg tgggaggagt cggtttcatt gattgtaccg tcgaaggcaa
     5641 agccggtacg acagcacata ttaagccggg agctaccggt gaaaacgaca ttggcacact
     5701 gaccatactc aaggaactct atctgaacag caacaattcc gtggatattg attttggccc
     5761 cggaggaaag tcggacaaac tgattttggt cggaggcggt gcattcagcg gcgaatttac
     5821 ccgtttatgg gtgaatatta tggatgactt ttacgataat cccgtagccg gcgattacaa
     5881 ggtattggat gcccctacgc tgaggatgaa cgaagttccc gtattagaca ctgtttttac
     5941 gggtactttt accacgacca tcgacagtat ttactcgggc gacacactga tccgggtcga
     6001 taccatcgtg actgccgaca ttgaacgcat agataccata ggctgggaaa tcagtaacaa
     6061 tctggttgtc aatgctaatc aattgcaaac cggtcaacac aaagattctc ttcccgaggg
     6121 ctatgcctat aacttcgatc gtttcttcag cgagggtatt attgccatta ccggaccggg
     6181 ctatgaggaa ggaaatattg tgaacggacc ctcaggcatc aatcaagtta aattgcccga
     6241 taaatttgta aaacagcgtg aaattttcaa cctgacgggt gtccgcacca aaacgctgac
     6301 ccggggtatc aatattatcc ggactacctt tagcgacggc agtgttgaag ttcaaaagta
     6361 cttttacgac gaatagttac aatcaatatt tttattattc attttaaatc taagcatgta
     6421 tgaaaaaatt ctgcgttttg ttattagcgc tgatgttctt acctacatgg ctcggagcgc
     6481 aaacagtctt cgataacact acggtaaatt caacctatgt tttatcggat accgttttgc
     6541 ccacagtggc caacgtagat acggcagagg ccttttcgac cacccagttt attatgggtc
     6601 cgggattgcg ggtaaaaact tttaagaaag tgtccagcga cttgacggcc actttgtttg
     6661 aagaacccaa tgctacagct ctgaccatag gcgggagatt acccgattat gtgctcactt
     6721 ttattttaag accctcgaaa ggcgttactt tcaaaccgac caacatctcg ttcaaagcct
     6781 ttaaaaatgg cacaggagat caggttaaaa tagacgttat gaatcaacgc ggcgaggctg
     6841 ccgagactct gttgtcgagc attgacattg ctcgtaacaa cgaagccgaa ggctactata
     6901 caaccgtatc gctcgatatt accgatggtg ttgcttcgaa cgacacgtat atcctgaagc
     6961 tttatccctg gggtaaaggt atgcgtgccg gcaaaggttg cggcattggt gatttggtaa
     7021 taaccggtgt ggtacacgga acggtgattc ccgtaccgaa atacaccatc actccacttg
     7081 tcagtcccga aggggcagga gaaatcagca ttaatcctcc cggaacgctt tttgatgaag
     7141 gcacggaact gaatctgata gccttgagaa actttggctt cgaattttcc aattggaaaa
     7201 gttcggaaac cctattgacg gattccagtg tgttggaatt ggtattgatg tcggatacca
     7261 cggttactgc ccattttacg cccgtcagta cgtacgagtt ggcaatcaat gctccggccg
     7321 gtgttaaaga ttacatgttt gatctggatc ctcctttctt tcctgttgcg ggtaaaaaga
     7381 tgtacgaaac aggtacctcg gtcttggtca gcccccgctc caacgccata atggacttta
     7441 caggctggga aggcggaagt gccggacccg atactacggt gaacatgact gccaatgtaa
     7501 ctcttcaagc caattacgat gtaaaaactt atttggccgg ttgggatttc tacgtggccg
     7561 gcaataaaga tcgtttcgcc gattttgcat cggcagagaa tgtggtcact aagcttatgc
     7621 ttatcaatgc cgatggtacg agcaccagtt ggctggataa gtccgaaatg ctgggagcca
     7681 gttacgaagg caaatattgt gccgtcaact ggaaacctct ggccgacaaa tactactacc
     7741 agacttgtgt caacgcctcc atgtacaaca acatcaaggt ggttgcttcc atgttgtata
     7801 attacaatgc ttaccaaaag caactggtag aatattcttt ggatggtacc aattttacca
     7861 ccctcgatac cattgtcatg tcgaccaaca aggtgtggta taccaagact gttgctttgc
     7921 ctcaggcagc cgattatgcc tctaaggtgt atatccgttg gatacccgac tacacctcgg
     7981 acatagtcgg tacgtcctcg accaacgacg gaacctcgat taccgatatt tttgtctatg
     8041 ccgaaggtct caaaaccgat gcaaaggcgg atatcaccga cactaccgtt gttaatccta
     8101 actttgatac caatatcaat ggttggacca gtaccaccgg agccagcagc aacaagattg
     8161 ctaccaacaa gaccggagac tttaccgggc ctttctggga aaactggaga aacaccagct
     8221 ttaccggcaa gatgtctcag attatcaccg gcttgcccaa cggatcttat ctgttgagag
     8281 ctgctgcttt ctgcaacaat gggggtgacg gcttctattt gtttgccaac agcggtaaaa
     8341 ctcaggttca gaccactgag ccgactttcc acgaggtggt aggcgtggtt accgatggta
     8401 agctcgaaat cggcttgctt gctgagctgc ccaccaacaa ctgggtaggt ctggacaacg
     8461 ttaaactcta cagcctgggc tttatattga acgatatgat tacggctctg gaagagctga
     8521 ttgttgaagc ggaagctatc accggcgata tgcaggccag cgtagctacc ggtttggccg
     8581 atgccatatc aaatgctgaa gccgctgtgg cctctcgttc ggaggtgcat gtggtggcct
     8641 cgttgaccgc tttgcgcgcg gctattgatg ctgcctacag ttctatcgct ttgtacgccg
     8701 cattcccggc tttgcagaca aaagccaatt atatctatag tgctaccgcg gccgaggctg
     8761 aggcattaca ggctgctatt acgacttcca acgatactta tgtggctaaa acagccaacc
     8821 agactactta cgacaacctg gtagctgcca tggtttcgta cagagccgcc aacgatgtta
     8881 ctgcttatgt ggtgaatccc gatatgaccg agggtaaaac agtaggctgg gcaagtgact
     8941 ttggtgaaca aacagccgcg tatcccggtt ttgaaggaag gttcctcgaa aaatgggttt
     9001 ctgctgccaa taatctggcc gatttcaatc tcagccagat tatatcggat cttcccaacg
     9061 gcacttatac cctgagtgcc tggtgtatag caaccaagca aaaccaggcc ggtgaagctg
     9121 ccaaaactta tgtaaagaac gtctatctct acgccaacaa cgattcgacg cctgtttcca
     9181 gcttaaacgg tgtacccgaa gaattcatga tcaatgtcac tgtggccgac aacaccctga
     9241 agcttggctt taccggtata aactgtacgg ccaactggat agccatagac aaggtaaggt
     9301 tgatgagcca cgatgtctct actttgctgg cagccaaaca agtatcgctg aatgccctga
     9361 ttggagaagc ggaagctgtg gttgccggag tggacagcgc catgttggga gccgagtcgt
     9421 ttaaatacgc tcctgccaag ctggacgcac tcaatgccgc cattgattcg gctaaggtct
     9481 ttgcaggtgc ttcggtaaac gagtacgtag ccatggaaaa cttcctgaca gcagctatga
     9541 acaatctcag acagatgaat cagccgctgg ccactcaatt tttctatatt gtacatagtt
     9601 cactgaacca cttggatgta gctgacggcg tcaagataaa gacagctgca gacactgctt
     9661 attatcagtg gtttaaattg cttccgatcg gttccggaga attgaatgcc tatcatttga
     9721 agaccctcga tgaaaaattt gtcggcttga cgggtaccaa caactgggat atgtctgcac
     9781 aagacgtagc ctcctccgac aataacgtca aatttattat cgaaatgata gacggcagca
     9841 aatacaagct cagaacgatc aaaggcttgt tgggtaccga cgcagttacc agcggatcgg
     9901 gtgtctacgg caataaagcc gcaggagcca attccgagtg gagcttagcc gaagtaggag
     9961 aggctgttta tcagtatcat aattcttcgg ttgagttgaa gactgttttg gacgaggcga
    10021 atgctcaata cgattctact ttgatagccg ccgatgagtt tgctgctgcc attgctgccg
    10081 cccaggctgt gtacgacaag ggaacaggtg caagtgtggc cgaactgcaa gccgctgttg
    10141 tagctttgaa cgaggctatc gccgcctttg aagaagcgaa tgaagtgccg ccgatcgtta
    10201 tcaacaaaga cgactacgtg gctttctaca gccaggatta cgaatttgta gctcaggctt
    10261 cggatgtatg gacctcgccc aacaatcagg caggtttctc cctggtcgaa gaagcaggca
    10321 acaagtacat caattacgat cacggcaacg tcaattccag aggtgcttac agcaacttcg
    10381 acattgatta tgccggtatt tcggagtatc tggtagagtt tgatctggca ttgacagccg
    10441 gaaataatca ggcaaccgaa tttgcactta agggaacgga ttttgcctac gccggtggta
    10501 atgtcaactg gggtgcagaa aaagggtatc tctttaaatt gtccaccacc aattctacta
    10561 cctggaccat tgtcggtagt ggtgccgaaa gctatacggt ggaactgaat gcgcttgaat
    10621 tctatcattt cctggctttg gtcagagccg atggaattca gctaacgatt accgatgctg
    10681 ccgattccgt tgttttggat aatgttgcca ttactttggg cagtgaaggt atcgaatgcg
    10741 taggtctgca ttatcaggca ggtcgttatc aatcgaagat gaagatagac aatatcttgc
    10801 tgagtaaacc tgtgccgctg accaaggtaa cctggtatat gcaggattac gaaaacgttg
    10861 cttctgccgc agatgtatgg acttctccca acaaccaggc cggttattcc ctggtcgaag
    10921 aagaaggtaa taaatacatt tattacaacc acggcaacgt caattcgaga ggtagttact
    10981 ctaattttga gatagacttt gccgatgtag ataagtacat tctggaattt gacctggcac
    11041 tgaaagctgg taataaccag gcaactgaat ttgcagttaa gggaactgat tttgcctaca
    11101 ccggtgataa tgtcaactgg ggtgcaggag gaggttatct ctttaaatta ttcaccacca
    11161 attctactac ctggaccatt gccggcagtg gtgccgaaag ccatactgtg gaactgaatt
    11221 ctctcgaatt ctaccattac agtgccgaaa taagcgccga aggcatattg ctgacaatta
    11281 ccaatgctgc cgattccgcc gttctcgaca aagctgccat cacttcgggc agtgcaggca
    11341 tcgaatgtgt gggtctgtat tatcaggcag gacgttatca atctgaaatg aagatcgaca
    11401 acatcgcttt cagtaaactg gtcaagggtg tcgttatggt agaacccacc gtgttgatga
    11461 caggagtgag cggaatatcc cgtatcatta ccatgctttc aacccagccc actgccatct
    11521 tgaagtatgt ccttgtcagt gccgaag
//
LOCUS       DAOSGL010000030        11545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4702_length_11545_cov_4.277981, whole genome shotgun sequence.
ACCESSION   DAOSGL010000030 DAOSGL010000000
VERSION     DAOSGL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4702_length_11545_cov_4.277981"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKY68_01905"
     CDS             complement(<1..809)
                     /locus_tag="PKY68_01905"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOH23826.1"
                     /translation="MKINIFDKKSTMRPWACISIILLLTTAPLGNAQNKSIARVPFTM
                     LGSYIVVKAKINESSGLHFILDTGIRNTILTEWHTNDSLSLVINEERYVQGLGGGDLI
                     FAYVSENNSIQLGKKLTFEKKTIVLLHEDVLELSRRIGSPINGLLGADLLEDYVVQID
                     FVAQRLIFYLKEDFKLPRKYKSLPLTIEQQKLYVPLKVKDNAKEYQLKMLLDTGAGLC
                     AWLRTIGSDVVPIPEKKVYGRIGEGISGEVTGYLARIPTVCLGDFCLSNPVV"
     gene            complement(1010..1819)
                     /locus_tag="PKY68_01910"
     CDS             complement(1010..1819)
                     /locus_tag="PKY68_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH23827.1"
                     /translation="MGLCLSACLQIEPRGFFVSYESVDQRFEQSMTWNDQHPFPTLVS
                     SEEEYEFYAVSDIHAGKTNNLGLFLEQALLQQPIAVFMAGDLTTGNANDYHELYAVLP
                     PQDSLPLFPIPGNHDLYFGGWKQYRLLFGSSVYSFSIKTSQTSDLYICLDNAGGTLGA
                     LQLEWLKELLLYERPQHRYCVLVNHNNWVRLRRMLATNPYVEELEVLLELCLKHRVDL
                     VISGHDHKSHTAVFGNTLFITLDALRDSYPTPSYLIVSIGPDAINYSYRNL"
     gene            complement(1837..2562)
                     /locus_tag="PKY68_01915"
     CDS             complement(1837..2562)
                     /locus_tag="PKY68_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23828.1"
                     /translation="MQNTYKALYLILLMMACCYPANSQVELDYLLDLGQTNVSQGLYI
                     STALVSAYSFDKYALKAGAQFQLIHPSSRVFSAAEFVAGRCFCVGKQTLEAETFYLYR
                     NFSSLMFISNWGLAVGIRKRHWRFDLGQDFKAYRVREKALSYETEGRVIRENFNLIYR
                     IAYFVNSPEKPWNISLSLTNRDYFLINQETNPMLSLRAETYVSSTMKLYLENWYKPAG
                     ALNMSAHYFGYFARTGFIWTPAF"
     gene            complement(2583..3773)
                     /gene="mnmA"
                     /locus_tag="PKY68_01920"
     CDS             complement(2583..3773)
                     /gene="mnmA"
                     /locus_tag="PKY68_01920"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOH23829.1"
                     /translation="MKEKIVLGLSGGVDSSVAAFLLKEQGYEVIAVYMINWKDTTGTL
                     SGACPYEEDVLIAELVARKLGIPFHVVDLSDTYRRQVVDYMFEEYARGRTPNPDVLCN
                     REIKFDAFLQTAQKFGADRIATGHYCRCDSVKAGDGKVYYRLLAGRDKNKDQSYFLCQ
                     LSQKQLSRAVFPIGELDKGRVRELAEELGLASAQKKDSQGICFVGKIDLPVFLQQKLG
                     SKKGRILEVPADFKAYHRSYPEDLEERIKKQARPYFYRASDGVLVGEHQGAHFYTVGQ
                     RKGLHVGGTEKPLYVLATDVENNLVFVGRGEDHPGLYRKALRINHQEVHWLRPDLALK
                     TGESADYSVRIRYRQALQPARLHCFEEALYIAFEEEQRSIVAGQFAVWYWGDELLGSG
                     VISA"
     gene            complement(3807..4265)
                     /locus_tag="PKY68_01925"
     CDS             complement(3807..4265)
                     /locus_tag="PKY68_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOH23830.1"
                     /translation="MEPIVFAYLGIGLMVGLTCVGSAIGVTICGNAVIGALKKNPDQT
                     GAYIGLSALPSTQGLYGFVGYFFMSSYLLPGMNWYAAAAIFGGCLGLGLSGLISAIRQ
                     ADVCANGITAIGSGHNVFGMTMVLAVFPELYAILALLVLILIRGLIPMPV"
     gene            complement(4328..6178)
                     /locus_tag="PKY68_01930"
     CDS             complement(4328..6178)
                     /locus_tag="PKY68_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOH23831.1"
                     /translation="MKKYSFLVYHAIYIDFLENLREIGVVDVIEKQLGHVEEHSELYE
                     KLEERKRINDLLKLLKQRFPEGLDQASVDTKANREDLLRRFEGILNEQTQLETKIQVL
                     NREISNLSVWGDFDPQRLEQLREAGCRIDFYFCPRSGFKPEWEEQYRALKIADSGNAV
                     YFITFSFDGQQPQIDAEHLRWPQKSLSELQLEVSALKARRLELEDQLKTEALNQYQTL
                     LYQDKLLSDQFNFSKVLIGTEKAAGDKIMILEGFVPCDREDKLKDLLSREGIYYLAEQ
                     PKQEDKIPVLLKNNRFARLFEPITEMYSLPNYSELDLTPLLAPFFMLFFGLCLGDGGY
                     GLLILLAATLIKPKLGENTRLFATLAQVLGFSTVLVGILTGSFFGVALESLEWSWLKG
                     VKQYFLTENNFGAMFGGYHPLMILSIVIGVVQILYAMCINVVKISIQLGFKYAMSNLA
                     WVVLLIASMVTFGLPALGVTLSVPLTYGLYGLLGLAVLIIVFYNSPGKNVFINVGSAI
                     WDTYNMASGLLGDTLSYVRLFALGLTGSILGSVFNSMAFGMTEAMPGWIRWLAVLLIL
                     LVGHGLNIALNVIGALVHPLRLTFVEFYKNAGFEGGGKAYNPFRKSAQTS"
     gene            complement(6187..6795)
                     /locus_tag="PKY68_01935"
     CDS             complement(6187..6795)
                     /locus_tag="PKY68_01935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOH23832.1"
                     /translation="MAIQYQYNKTSLQNLEKQLKIRERALPTIKNKESALRLEVKKAK
                     AETRELEKKLEMLIENYREMHALWTEFNAELIKVKDVELGVRKVAGVRIPVLEEVLFE
                     IAPYSMFHAPKWYAGGLDLLKKLATAGIEQDFSVRKLQLLEYARKKTTQKVNLFEKVQ
                     IPGYQDAILKIKRYMEDEENLSKSSQKIVKTRQNAQKQEESL"
     gene            complement(6799..8124)
                     /locus_tag="PKY68_01940"
     CDS             complement(6799..8124)
                     /locus_tag="PKY68_01940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOH23833.1"
                     /translation="MATKAFQKIYTKITQITKATCSLKAANVGNEELATVDGKLAQVV
                     KIIGDEVTLQVFGGTEGLRTNAEVVFMGKAPSLNVGDQLIGRFFNAFGDPIDGGPVPE
                     GKEVEIGGPSVNPVRRKQPSELIATGIAGIDLNNTLVSGQKIPFFADPDQPYNQVMAN
                     VALRAQTDKIILGGMGLTNDDYLYFKNVFSNAGVLDHIISFVNTTEDPPVERLLIPDM
                     ALTAAEYFAVEKNEKVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPDGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDTDVGKVIVDPFR
                     SLSRLKQLVIGKKTREDHPQLMNAAVRLFADAANAKTKLENGFDLSDYDERCLDFAKE
                     YSAQILAIDVNIDTTEMLDTTWKILAGHFRVEELNIRQDLVNKYMPKVS"
     gene            complement(8136..9893)
                     /locus_tag="PKY68_01945"
     CDS             complement(8136..9893)
                     /locus_tag="PKY68_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOH23834.1"
                     /translation="MSTKGIVKGIISNLVIVQVDGPVSQNEICYILHKEARLMAEVIK
                     VVGQQAYVQVFESTRGLKVGFEVEFSNHMLEVQLGPGLLSRNLDGLQNDLDKMQGIFL
                     NRGEYTSPLDEKQLWEFQPLANAGDKVRAADWLGETKENFQAHKIMVPFVLEGSYTVK
                     SIVPQGSYTIKETIAVLLDEGGNEIPVTMVQRWPVKKALKAYKEKPRPFRLLETGVRI
                     IDIANPIVEGGTGFIPGPFGTGKTVLQHAISKQAEADIVVMAACGERANEVVEIFTEF
                     PRLEDPHTGRKLTERTIIVANTSNMPVAAREASVYTAMTIAEYYRSMGLKVLLMADST
                     SRWAQALREMSNRLEELPGQDAFPMDLSAIISAFYGRAGYVILNNGQTGSITFIGTVS
                     PAGGNLKEPVTEGTKKAARCFFALEQIRADRKRYPAINPIDSYSKYIEYPEFEAYIAE
                     NLSDEWIPTVNEMKTLLQRGKEVQEQINILGDDGVPLDYHITYWKSEVIDFVILQQDA
                     FDKVDALCPIERQQYMLKKVMGITRLNFEFDNFTEVMDYFKRVIHVLRQMNYSEFQSE
                     PFRQLETELEAILKERVTQ"
     gene            complement(9911..10753)
                     /locus_tag="PKY68_01950"
     CDS             complement(9911..10753)
                     /locus_tag="PKY68_01950"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOH23835.1"
                     /translation="MSQYHYLVAGFPDISLDDNKLQLTYIAFIEELLRSFSGRDADLL
                     RLFRLDYDNENLLSWINNKEAEFHPLGLISPEDLKEQMELLDYDERAPLKGIPPYFLT
                     FLREWKSEQLEAGKEKKRLTELYYDYVGQAPNKFVREWFEFELNMNNILTALNCRKYR
                     LPVAGQIIGNNAIAESLKISTVRDFNVSELFSEMDSLLAIADEENLLEREKMIDRLKW
                     DYLDEHSVHYYFSVEVLLVFLIKLKSLERWLSLDPKSGEESFRSLVNSLRTSVRMPAD
                     AHIK"
     gene            complement(10756..11343)
                     /locus_tag="PKY68_01955"
     CDS             complement(10756..11343)
                     /locus_tag="PKY68_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23836.1"
                     /translation="MNSIKDLTDQLFQEGVEKGRKEAQQLIDEAEKKKSELIAAARQE
                     AAQIIEAAKKQAQELDKNTKAELQLFARQALDALKSEIVNLLTDKVVREAIQTSSNDK
                     TFMQNLLLVFVKNWAENGELEIQTSEAKALTDFFKAKAKELLDKGLTIKQVNNLDTHF
                     ILTAKDGSYKIQFGEEEFINYFKEFLRPQLVEMLF"
BASE COUNT         3070 a         2807 c         2532 g         3136 t
ORIGIN      
        1 accaccggat ttgaaaggca aaaatcgccc agacatacgg tgggaattct ggccagatat
       61 cccgttactt cgccactgat gccttcgcct atacgcccgt aaaccttctt ttcgggtata
      121 ggcaccacat cgcttccaat ggttctaagc caagcacaaa ggccagcccc cgtatcgaga
      181 agcattttta gttgatattc tttggcattg tctttgactt ttaacggcac atagagcttc
      241 tgctgttcta tggtcagggg cagactcttg tattttcggg gtaatttgaa atcttctttg
      301 agataaaaaa ttaagcgctg tgcaacaaaa tcaatctgca ccacgtaatc ttctagtaaa
      361 tcggcgccca gcaaaccatt gattgggcta ccgattcgcc ggctcagttc caatacgtct
      421 tcatgcaaaa gaactatcgt tttcttttcg aaagtcaatt ttttccctaa ttggatactg
      481 ttgttttcgc tgacgtaggc aaatataaga tcgccccctc ccagtccctg aacatagcgt
      541 tcttcgttga ttaccagcga caaactgtcg ttggtatgcc actcggtgag gatggtgttg
      601 cgtatgcccg tatccaaaat aaaatgcagg cccgaagatt cgttgatctt ggctttgacc
      661 actatataac tgcccagcat agtaaaagga actctggcaa tgcttttgtt ttgagcgttc
      721 cctaaagggg ccgttgtcaa cagcaggata attgaaatgc aagcccaagg cctcatggtg
      781 gattttttat cgaagatatt tattttcatt caataaatga atctcatctt catcccgtga
      841 aaaaaatcct ttccggaaac ggtatatccg tacggctctt cttcatctca tctccatccc
      901 cgcgaaaaag tccccggcat agagaagttc ccggactgtt aataatgaag aactcccggc
      961 ttatgaacaa tggtggactc ccacctatca acaatgggga agtcccggcc tataaattac
     1021 gataggaata gtttatagca tcggggccga tacttacaat caaatacgag ggagtaggat
     1081 atgaatctct cagggcatcc agggtgataa agagggtatt tccgaatacg gcagtatgac
     1141 ttttgtgatc gtgtccgctg atgaccagat ccaccctgtg tttgaggcat aattccaaca
     1201 aaacctccaa ttcttccaca tagggattgg tggccaacat tctgcgcagc ctgacccaat
     1261 tgttgtgatt aaccaataca caatagcggt gttgaggacg ctcgtaaagc agcaattcct
     1321 ttaaccattc cagttgcaag gctcccaaag ttccgcccgc attgtccaaa cagatataca
     1381 agtcactggt ctgtgaagtt ttaatactga aactgtaaac cgacgatccg aataaaagtc
     1441 gatattgttt ccagcccccg aaatagaggt catgattgcc cggtatcggg aaaagcggca
     1501 atgaatcctg aggcggcaaa acggcataca gttcatgata atcgttggca tttccggtag
     1561 tcaggtcacc tgccataaaa accgctatgg gttgctgcaa caaagcctgc tctaaaaaca
     1621 agccgagatt gttggtttta cctgcgtgaa tatcggaaac ggcataaaac tcgtattctt
     1681 cttcggagct gaccaatgtc ggaaaaggat gctgatcatt ccaagtcata gattgctcaa
     1741 aacgctgatc tacagattca tacgaaacaa aaaaacctct gggttcaatc tgcagacaag
     1801 cagaaagaca caggcccata cagcacaagc ttaatctcag aatgcaggag tccatataaa
     1861 tcctgttcta gcaaaataac caaaataatg ggcactcata ttaagtgctc ctgccggttt
     1921 ataccaattt tcgagataaa gtttcatggt agaactaacg tatgtctcag ccctaaggct
     1981 caacataggg ttggtttcct gatttatcag aaaataatct cggttggtca ggctcaaact
     2041 aatgttccag ggcttttcgg gactgtttac gaaataggca atgcggtaaa tcaaattgaa
     2101 gttttcgcga atcacacgac cctccgtttc gtaagacaaa gccttctccc ggacacgata
     2161 cgccttaaaa tcctggccca agtcaaatcg ccaatgtctc ttccttatac cgacagccag
     2221 gccccaattg gaaatgaaca tcagactcga aaaatttcga tacaaataaa atgtctccgc
     2281 ttcaagggtt tgtttcccga cacaaaagca acggccggca acaaattcgg ctgccgaaaa
     2341 tacccggctg gagggatgga tcagttgaaa ctgtgcaccc gccttcagag catatttatc
     2401 aaaactgtag gcgcttacta aggccgttga aatgtataag ccctgcgaca cattggtctg
     2461 ccccaaatcg agcaaataat ccagctcaac ctgagaattg gcaggataac aacaagccat
     2521 cattaaaaga ataaggtaaa gggctttgta ggtgttttgc attggtatat tctcaaaaaa
     2581 tattaagcag aaatcacccc cgaacccagc aattcatcgc cccagtacca aacggcaaac
     2641 tgtccggcca cgatgctgcg ctgctcttcc tcgaaagcaa tatacaaagc ctcttcgaaa
     2701 caatgcaaac gggccggctg taaagcctga cgatagcgga tacgcaccga ataatcggca
     2761 gactctcccg ttttcaatgc caggtcgggc ctgagccaat gcacttcctg atgattgatt
     2821 ctgagtgctt tccggtacaa acccggatga tcttctcccc tgcccacaaa gaccaggttg
     2881 ttttctacgt cggtggccaa aacataaagc ggtttttcgg ttccgcctac atgcaagcct
     2941 tttcgctgtc ctacggtata aaaatgagcc ccctgatgtt cgcctaccaa tacgccatcg
     3001 gaagcacgat aaaaatacgg acgggcttgc tttttgattc tttcttccag gtcctcggga
     3061 taagagcgat ggtaggcttt aaaatcggca ggcacctcga gtatccggcc ctttttactg
     3121 ccgagttttt gttgtagaaa aacaggcaaa tctattttgc cgacaaagca aatgccctgc
     3181 gaatcctttt tttgtgccga agccagtccc agttcctcgg ccagctcacg cactctgcct
     3241 ttgtcgagtt ctccgatggg gaaaacggct cgtgagagtt gcttctggct caattgacat
     3301 aaaaagtaag attggtcttt gtttttgtcc cgaccggcca acaggcgata atacacttta
     3361 ccatcgccgg ctttgacgct gtcgcagcga caataatgcc cggtagcaat cctgtcggca
     3421 ccaaacttct gtgcagtttg caaaaaagcg tcaaacttga tttccctgtt gcaaagcacg
     3481 tcgggattgg gtgtcctgcc ccttgcgtac tcctcgaaca tatagtcgac cacctgacga
     3541 cgataagtgt cgctcagatc aaccacatga aagggtatcc ccagtttgcg ggctaccaat
     3601 tcggctatca acacatcttc ttcgtaggga caggctcccg acaaagtgcc ggtagtgtcc
     3661 ttccaattaa tcatgtaaac ggcaataacc tcatagccct gctctttgag caagaaagcg
     3721 gcgacgctcg agtcaacgcc gccggacaag ccaaggacaa ttttttcttt caatgccgat
     3781 gtgttttatc cgctttcaaa gcccgatcaa accggcatag gaatcagacc acggataaga
     3841 atcaatacca aaagcgccag gatggcgtaa agttccggaa aaacggccaa gaccatggtc
     3901 ataccaaaca cattatgacc cgaacctatg gctgtgatac cgttggcaca aacatcggcc
     3961 tgacgtatag ccgaaatcaa accggacaat cccaatccca gacaaccgcc aaagatagct
     4021 gcagcagcgt accagttcat tcccggcaac aaataggaac tcataaagaa atagccgaca
     4081 aaaccgtaaa gcccctgagt ggaaggcaaa gcagacaaac cgatataagc tcctgtttgg
     4141 tcggggttct tttttaaggc tccgatgaca gcgttaccgc atatggttac accaatggca
     4201 cttcccacac aagtcaaccc gaccatcaat ccaattccga gataagcaaa tacaataggt
     4261 tccatagttt gagttttaaa tttagacgat aatattcatt gttttaataa ttccattgag
     4321 agtgatttca actggtttgg gccgattttc taaagggatt gtaggctttg cccccaccct
     4381 cgaaaccggc attcttataa aattccacaa aagtgagacg caagggatgc accaaagctc
     4441 ctatgacgtt caaggctatg ttcaaaccat gccccaccaa caaaataagc agtacagcta
     4501 accagcgtat ccagccgggc atggcttcgg tcattccaaa ggccatggaa ttgaatacag
     4561 atcccagaat actgcccgtc aggcccaaag caaataaacg cacataagaa agtgtgtcgc
     4621 ccagcaaacc ggaggccata ttgtatgtat cccatatcgc ggacccgacg ttgatgaaaa
     4681 catttttgcc gggagaattg taaaacacaa ttatcaggac agccaggccc agaagaccgt
     4741 ataaaccgta agtaagaggc accgacaacg tcactcctaa agcaggcaaa ccaaaagtga
     4801 ccatggaggc aataagcagc accacccaag ccagattaga catagcgtat ttaaatccca
     4861 attgtatgct tatcttaacc acattgatgc acatggcgta caaaatttgt acaaccccta
     4921 tcacaatcga caaaatcatc agcggatggt agccgccaaa catggccccg aaattatttt
     4981 cggtcagaaa atactgtttt actcccttaa gccatgacca ttcgagcgat tccagagcta
     5041 ctccgaaaaa agacccggtc agtatgccaa ccagcacggt cgaaaatccc aggacctgcg
     5101 ccaaagtggc aaataacctg gtgttttcgc ccaattttgg tttgatcaga gttgcggcca
     5161 gcagaatcaa caaaccatag cctccgtctc ccaggcaaag tccgaaaaac aacataaaaa
     5221 agggagccag caagggggtc agatccaatt ccgaataatt tggcagggaa tacatttcgg
     5281 ttatgggctc aaaaagccgg gcaaaacggt tgtttttgag caaaacaggt attttgtcct
     5341 cttgtttcgg ttgttcggca aggtaataaa tgccttcgcg gctgagtaag tctttgagtt
     5401 tatcttccct atcgcagggc acaaagcctt cgagtatcat tatcttatcg cccgctgctt
     5461 tttctgtccc aatcaagact ttggaaaaat taaactgatc gctgagcaat ttatcctggt
     5521 acaaaagggt ttggtattgg ttcagggctt cggttttgag ttgatcttcc aactccagac
     5581 ggcgagcctt taaagcggac acttccaatt ggagttcgga aagcgacttt tgcggccagc
     5641 ggagatgttc cgcatcgatt tggggttgtt gcccgtcgaa gctgaacgtg ataaaataaa
     5701 cggcattccc gctgtcggct attttcagag cgcgatattg ctcctcccac tcgggtttga
     5761 aaccgcttct gggacaaaaa tagaaatcga tacggcaacc ggcttcccgg agttgctcca
     5821 gacgttgggg atcgaaatca ccccaaaccg acaggttgct gatttcacgg tttaagacct
     5881 ggattttggt ttccaactga gtttgctcat tcagtatacc ttcaaagcga cggagcaaat
     5941 cttcgcggtt ggccttggta tcaacagaag cctgatcaag tccttcggga aaacgctgtt
     6001 tgagcaattt cagcagatcg ttgatgcgct tacgttcttc gagcttttcg tacaactcgc
     6061 tgtgctcttc cacatgaccc aattgctttt cgatcacatc gaccacgccg atttccctga
     6121 ggttttcgag aaaatctatg taaatggcat gatagaccaa aaagctgtat ttcttcatcg
     6181 gaacaatcat agactttcct cctgcttttg ggcattttgc cgtgttttta ctattttttg
     6241 cgaagacttg gataaattct cttcgtcttc catatagcgt ttaatcttta atatggcgtc
     6301 ctggtaaccg ggaatctgaa ctttttcgaa taaattcact ttttgagtgg ttttttttcg
     6361 ggcgtattcc aacaattgca gcttccgaac gctaaagtcc tgttctatac cggctgtggc
     6421 caattttttg agtaaatcca atcctccggc ataccatttg ggagcatgaa acatactgta
     6481 gggggcgatt tcaaacagca cctcttccag taccggaatt cgtacgccgg ctacttttcg
     6541 cacacccaat tcaacatctt ttaccttgat aagctcagcg ttaaattcag tccacagggc
     6601 gtgcatctcc ctgtagtttt cgatgagcat ttcaagtttt ttctccaact ctctggtttc
     6661 ggctttggcc ttcttcactt caagacgcag ggcgctttcc ttgtttttga tggtaggaag
     6721 agctctctca cggatcttga gttgcttttc aagattctgc aaggacgtct tgttgtattg
     6781 atattgaata gccatacttt acgaaacttt gggcatatac ttgttgacca ggtcttgtct
     6841 gatgttgagt tcttccaccc tgaagtggcc ggccagaatt ttccaggtgg tatcgagcat
     6901 ctcggtggta tcgatattga cgtcgatggc cagtatttgg gctgaatact ctttggcaaa
     6961 atccaggcag cgttcgtcgt aatcgctcag gtcgaaaccg ttttccagtt tggttttggc
     7021 attggcggca tcggcaaaca aacgcacagc ggcattcatc agctgaggat ggtcttcgcg
     7081 ggtcttcttg ccgatgacca gttgtttgag ccgggacaaa ctgcggaagg gatccacaat
     7141 aactttgccc acatcggtat cacgacgcaa aaacaactgt ccttcggtga tataaccggt
     7201 attatcggga atggcgtgcg taatatctcc tcccgaaagg gtggttaccg caataatggt
     7261 aatggaacct ccgtcgggga actgtaccgc tttttcgtaa atcttggcca ggtcggaata
     7321 cagagaacct ggcatggaat ctttggaagg aatctggtcc atacggttgg atacgatcga
     7381 aagcgcatcg gcatacaggg tcatatccgt aagcaacacc aatacttttt cgttcttctc
     7441 cacggcaaaa tattcggccg ccgtcaaagc catatcgggt atcagcaaac gttccaccgg
     7501 aggatcttcg gtggtattga caaaactgat aatgtggtcg agcaccccgg cgttactgaa
     7561 aacatttttg aagtacagat aatcgtcgtt ggtcagcccc atgccgccca aaatgatctt
     7621 gtctgtctgg gctctgaggg ctacgttggc cattacctga ttatagggct ggtcggggtc
     7681 ggcgaaaaaa ggaatctttt gtccggaaac cagggtgttg ttcaggtcta tgccggcgat
     7741 accggtggca atcaattcgg agggttgttt gcgccgtacc ggattgaccg aagggccgcc
     7801 aatttcgact tctttgcctt cgggcaccgg ccctccgtct ataggatcgc caaaggcatt
     7861 gaaaaagcga ccgatgagct gatcgccgac attaaggctg ggtgccttgc ccataaagac
     7921 tacttcggcg ttggtgcgca aaccttcggt gcctccgaag acctgcaaag taacttcgtc
     7981 tccgataatc ttcaccactt gcgccaattt accgtcgaca gtggccaatt cttcgtttcc
     8041 gacattggca gctttcaggc tacaggtggc tttggtgatt tgggtgattt tggtgtatat
     8101 cttttgaaat gcttttgttg ccattatccc ggattttatt gtgttactcg ttcttttaat
     8161 atcgcttcca gctcggtctc caactgccta aacggctccg attgaaactc ggaatagttc
     8221 atctggcgaa gtacgtggat aactctctta aaataatcca tcacctcggt aaaattatca
     8281 aattcgaaat tcaaacgggt tatgcccatc accttcttga gcatatattg ctgacgctca
     8341 atgggacaaa gagcatcgac cttgtcaaaa gcatcctgtt gcaaaatgac aaaatcgatc
     8401 acttccgatt tccagtaagt aatatgataa tcgaggggca ccccgtcgtc tcccagaata
     8461 ttgatttgtt cctgcacctc cttgcctctt tgcagcaggg ttttcatttc gttgaccgtg
     8521 ggaatccatt cgtccgacaa attttcggca atgtaggctt caaattcggg gtattcaatg
     8581 tacttggaat acgaatcaat aggattgatg gccggataac gtttacggtc ggcccggatt
     8641 tgttccagcg caaagaaaca acgtgcggct tttttagttc cttcggtcac cggttcctta
     8701 aggttaccgc ctgcgggcga aaccgtaccg ataaaggtga tggatcccgt ctgtccgtta
     8761 ttgaggataa catagcctgc acgcccgtaa aaggctgaaa taatggcgga aaggtccata
     8821 gggaaagcgt cctgtccggg caattcttcc agacggttgg acatttcgcg gagcgcctgc
     8881 gcccaacgcg aggtggagtc ggccatcaac aacactttca aacccatgga acgataatac
     8941 tcggctatgg tcatggcggt atacaccgaa gcttcgcggg cagccacagg catattggaa
     9001 gtattggcca cgataatggt tctttccgtc agtttgcgtc cggtgtgggg atcttccagc
     9061 ctgggaaatt cggtaaagat ttccactact tcgttggctc tttccccgca agccgccata
     9121 accactatgt cggcctccgc ctgtttggaa atagcgtgtt gcagcaccgt tttcccggtt
     9181 ccgaaaggtc cgggaatgaa gccggttccg ccctcgacta taggattggc aatgtcgatg
     9241 atacgcacac cggtttccag cagccggaaa ggcctgggtt tttctttgta agctttgagt
     9301 gcttttttta ccggccagcg ctgtaccatg gtgacgggta tttcattgcc gccttcgtcg
     9361 agcaaaaccg cgatggtctc tttgatagta taactgcctt ggggtactat acttttgacg
     9421 gtatagctgc cctccagcac aaagggaacc attattttgt gcgcttggaa attttctttg
     9481 gtttcgccta accagtcggc agcccgaacc ttgtctccgg cattagccaa aggctgaaac
     9541 tcccataatt gcttctcatc caggggtgaa gtatattcgc ctctgttcag aaaaataccc
     9601 tgcattttgt cgaggtcgtt ttgcagtccg tccaaattgc gggacaataa gcccggaccc
     9661 aactgaactt cgagcatgtg gttactgaat tcaacctcga atcccacttt caggccgcgg
     9721 gtgctttcga acacctgaac ataagcttgc tggccgacca ccttgatgac ctcggccatc
     9781 agcctggcct ctttgtgtag aatataacaa atttcgtttt ggctcaccgg gccatctacc
     9841 tgtactataa ccaggttgga aatgatcccc ttgactattc cttttgttga catatcagtg
     9901 ttttagtaat ttactttatg tgtgcatctg ccggcatacg gacgctggtt ctaagtgagt
     9961 ttaccaggga acggaaactt tcctccccgc ttttgggatc gagcgacaac caacgttcca
    10021 ggcttttcaa cttgattaaa aaaaccagca aaacctcgac cgaaaaataa taatggacac
    10081 tgtgttcatc caggtaatcc cattttaaac ggtctatcat cttttctctc tccaagaggt
    10141 tttcttcgtc ggcaatagcg agcaagctgt ccatttccga aaaaagctca gaaacattga
    10201 aatcgcgcac ggtagatatc ttcaatgatt ctgctatggc gttgtttccg attatttgcc
    10261 cggcaacagg caaacgatac ttgcgacaat tcaatgccgt aagaatattg ttcatattca
    10321 attcaaactc gaaccattcc cttacaaact tattgggagc ttgcccgacg tagtcgtaat
    10381 aaagctcggt caagcgtttt ttttctttac cggcctcgag ttgttcagac ttccattcgc
    10441 gcagaaaagt gaggaaataa ggcggaatac ccttaagagg agctctttcg tcgtaatcga
    10501 gcaattccat ttgttccttg agatcttcgg ggcttatcag gcccagcgga tgaaattcgg
    10561 cttctttgtt gttgatccag gaaagcagat tctcgttgtc gtaatccagg cggaacaagc
    10621 ggagtaaatc cgcatctctg ccggaaaaag acctcaacaa ttcctctata aaagcgatgt
    10681 acgtcagctg cagtttattg tcatccaagg aaatgtcggg aaaacctgct accaggtaat
    10741 ggtattgact cataactaaa acaacatttc caccagttgt ggacgtaaaa attccttgaa
    10801 ataattgatg aattcttcct ctccgaattg aattttgtaa gaaccgtctt tggcggtcaa
    10861 tataaagtgg gtatccagat tgtttacttg ttttattgtc aaacctttat ctagaagctc
    10921 ttttgctttt gccttgaaaa agtcggtcaa agctttggct tcgcttgttt gaatttcgag
    10981 ttctccgttt tcggcccagt ttttaacaaa aaccaataat aaattttgca taaacgtttt
    11041 atcgttgctg cttgtttgga tggcctctct cactactttg tcggttagta agttgacaat
    11101 ttccgacttt aaagcatcca gggcctgacg ggcgaacaat tgcaattcgg ccttggtatt
    11161 tttatccaat tcctgtgcct gttttttggc agcttcgata atttgagcag cctcttgccg
    11221 ggcagctgca ataagttcgg atttcttttt ttcggcctcg tcaataagct gctgggcttc
    11281 tttccggcct ttttccacac cttcctggaa caactgatcg gttaagtctt ttatcgaatt
    11341 cataagattg ttgaaatatg atttagagcc acaatattac taatttattt actgcaccgc
    11401 aatgcaatta agtaaaaaac attctatgta ttggcaaatt ataacataaa aaaatctccc
    11461 ggctttctac cgggaggttt ttttacttct gtcgccaaca aaaccgacag aaattaaaga
    11521 tgatcaggat acgaaattta ttctg
//
LOCUS       DAOSGL010000031        11516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4722_length_11516_cov_4.161068, whole genome shotgun sequence.
ACCESSION   DAOSGL010000031 DAOSGL010000000
VERSION     DAOSGL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4722_length_11516_cov_4.161068"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2643
                     /locus_tag="PKY68_01960"
     CDS             <1..2643
                     /locus_tag="PKY68_01960"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOH23837.1"
                     /translation="NPRLSWRLDTEKENSLQTAYRIKVASTPEALKRNQLVWDSDVVS
                     SDRSVFVEYQGPQLVSGQRLYWQVTVWDNQGNQATSGETAYWEMGLLTSGEWKAAWIT
                     PGWKEDTSQSNPCPFFRKEFNVKKQVAKARMYASALGLYELYLNGERLGNDFFNPGWT
                     TYSKRIQYQVYDLTALLKQGRNALGAIVGDGWYRGFFGFQGERNLYGDKAGLITQLEI
                     NYEDGSKEWILSDPSWKSGKGPLLKSEIYHGEFYDARLEILGWAEPGFDDSKWEKVTT
                     LNQSNEVLVPTEGEPVRITETLKPIKKFVTPRGELVFDFGQNLVGWVRFRLKGERGQS
                     IVLHHAEVLDQEGNFYTDNLRVAKAEDVYIFKGEGVEEYEPRFTFHGFRYLRVSEYPG
                     EIGLDDVEGRVVHSDMEKIGEFECSDPLINRLQQNIQWGLRGNFLDVPTDCPQRDERM
                     GWTGDAQAFAPTACFNMNTGAFFTKWMKDLQLDQKADGNVPWVVPNVLEGGGGTGWSD
                     GYGGTGWADAAVIVPWTVYQSYGDVQILENQYESMKAWVEYMIRHSGSRYIFDYGFHF
                     GDWLSFAEYYTYNYNAPDYGYAGAHTEKDLIATAYFYYSTSLMSKIASLLGRNEDSER
                     YAGLLPRIKEAFHREFITPNGRLVSGTQTAYVLALGFDLIPEEEREAMAKRLADDVRY
                     FGHLTTGFLGTPLLCRVLSDNGYPDLAYQLLFNKRYPSWLYPVTMGATTIWERWDGIK
                     PDGSFQTVGMNSFNHYAYGAVGQWLYSTVAGLNIDPSKPGYKHSIIRPCPTRRLNYAR
                     ASLQTMYGLLLSSWEIKGDDIIYKFRIPPNTTATIYLEAEKINYSQKAQDLSGIKKMG
                     DKQFVIEAGSGSWEFVAHKAVINN"
     gene            2669..3961
                     /locus_tag="PKY68_01965"
     CDS             2669..3961
                     /locus_tag="PKY68_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH23838.1"
                     /translation="MSEASSKDLVELKENHSHPEKITKEQWKASALAGMASYLDAGSI
                     VALGAGLALFQEYLNLSNSAVGLLAAIGPNAIGCAIGSIIGGRLGDKWGRKRIYQIDL
                     LVYAFGVLLITFCFNATMLFVGTFIVGVAVGADVPTSLAFVGEVAPDKARGKLLGFTQ
                     VAWSFGPVVVLWLAMFLADLGVTGIRIVFLHLFVVALVTWYLRRSLSESARWTKAAKT
                     KGSQLRLLLKGSNLRGMAYTATIYLFWNLAAGTAGIFNPYIIKTLNAGSSQALSVGLS
                     SAGFIISIIVTSLVFMRYSDRNFKTRKLLWGIGAVMQVVAYSLFLFLPFTIPVIIANI
                     LMFSSGGALAGEAFYKVFSQELFPTMLRGTAQGITFGISRTILGVWSFFVPILAKTGI
                     GPVAALLSVFLLISGSVGFFFMPDTSGKSLEQIAKERA"
     gene            4001..5797
                     /locus_tag="PKY68_01970"
     CDS             4001..5797
                     /locus_tag="PKY68_01970"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOH23839.1"
                     /translation="MKLLFQSMGILSVLIICTTGCSPTKSKQWAHLRQDTLYIKASQF
                     RAGENTIALGDSANLIHFVIAEKDRVPFNQSVVKQTNEQAAYGPDAKLPYFTVRFAVP
                     IPPAYTPFEVGTLAGLEHGVYHHNHSPGFEIMPNGDALAVFFSSPAGKSESDTAATFV
                     QARLRHGSEDWDMPEVFFDTQFGNDQSGLLWNDKGKIWFFGGGRYISDYVPFRIAVST
                     DNGASWTFSVPQLDTAATDYTAQPVVNAFRDPQGGIYMVCDGDGSESFLWYSADEGKS
                     WKDKGGRTGSRHSTILPLDDKGTLLAQGGKNADIEGWNVRNISHDWGASWEEGTSSPF
                     PPLGTAQRPSMIRLASGCLFYVTDSYMHKKNIAPPAGWEFGNNAVVAISKDNGETWHI
                     KPLPVQLPQRHRPPHGSLGYSTARQAPNGVIHILTTANFPPIHYELNEAWIWSDLADI
                     TPESDGGEVKSFTETYKDGRLKSAWSARICPNGRYLLHGETTDYYENGAIQHVAVYEN
                     GRKSGEELYYDKEGKLEWKWQRDLHTNTGVWTQYWPNGNKKVESVWNIRPQARDLNRA
                     FFGYVAEGPSTHWDSDGNLVKVYQFSKGLLQE"
     gene            complement(5863..7239)
                     /locus_tag="PKY68_01975"
     CDS             complement(5863..7239)
                     /locus_tag="PKY68_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOH23840.1"
                     /translation="MKKIIILVCLCMAFLSANAQQKKIWNESPEEKKERLAWWTNDRF
                     GLFIHWGPYSLAARHEWVKKREQMDDKSYQKYVDHFNPDLYQPREWAKLAKTAGMKYA
                     VLTSKHHDGFAMFDTKFSDYKITNTPYGKDALKEWVEAFREEGLKIGFYYSLLDWHHP
                     EYTIDVKHPQSPENKEDYERLNRCRDMSVYREFLKNQVTELLTNYGKIDLLFLDFSFP
                     EEEFGKGCEDWGSVELIQLVRKLQPGIIVNDRMDLSDYWGGWDFATPEQHKVDKWPTY
                     KGEKIPWETCQTFSGSWGYYRDELTWKDNKQLLVLLIESVSKGGNLLLNVGPTGRGTI
                     DYRAENALLKIGDWMKYNARSIYGCTQAPDSFETPENTLLTYNPTTNRLYVHLLDYPL
                     RNITLKGMKGKVNYLQFLHDASEIKISTSKGFIAIDQGSTEDNDLRLSLPVNKPPLEI
                     PVIELFLN"
     gene            complement(7320..8609)
                     /locus_tag="PKY68_01980"
     CDS             complement(7320..8609)
                     /locus_tag="PKY68_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOH23841.1"
                     /translation="MRSFLFTIVVSFILSLPVCSQPLAFPGAEGYGAKALGGRGGMVL
                     FVSNLNDSGPGSLRQAVEQEGPRTVIFNVSGTIELESDLTIGNPYLTIAGQTAPGDGI
                     TLKNYKIKLAADHVILRYLRVRRGNLSEKQDDAITIQNAENVIVDHCSFSWGTDEVVN
                     TWHGSKNLSLQWCIIAEGLHHKEHGFAATLGGINTSYHHNLIANCPGRNPSVGGNHNY
                     QTHRMDFRNNVIFNFGHRTFDGKPSSINIVNNYFKPGPNSSIDVFARIDEAGVYKEIP
                     ESSWYIEGNVWEGNESISRDNSLGVKGATQWMVDQPNEFSAITTEQAEAAYLSVLAGA
                     GACFPKRDGVDLRILEEARTGNTTYDKGVVMSTSEVGGWPVLNSLPAPVDSDRDGMPD
                     EWETKHGLDPSNPEDRNTTNAEGYTMLEHYLNGLVQN"
     gene            complement(8677..>11516)
                     /locus_tag="PKY68_01985"
     CDS             complement(8677..>11516)
                     /locus_tag="PKY68_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOH23842.1"
                     /translation="ILAARAVVLPGVGAFPRDTVDRRIIHEVTTCTASAHGTFNNHRV
                     CGIINKPSDAGGYPVYNTYNQVQDEDHDGMADDWELANGLDPSNPDDRNLILKSGYTA
                     LDAYLCSLVGEDIPVETAKPYDIVVAKDGSGDFTSINAAIETVPENAGRKTIFVKKGV
                     YEEKVFIGNRWQDSRKVISMIGENVDSVVIVWEDYHGKTITYPGREGTIVADGMTAAT
                     FTVTAPDFYMENITVKNPSKAAQAEAIYQSGDRQVLKNCKILGNQDTHRTKKGRRYFY
                     YQCTIEGGVDFIYAGGTCYFYQCKIVSNRNGYITAPEDIVYTARLSNGKILYYRFIFN
                     DCDLLAKDGANDVYLGRPWSSESGSIFMNCRLGSHINRAGWNAWGGNEQNCSFAEYKS
                     LNADGSALADVSGRVSWSMQLSDKDRYNLLGMNTIYRAVSSSAFDPVSQFLPFSGPKS
                     LTVSNRKLSWTAVENAVGYVVYANDAAIDFAETNSYYDLVDRGTVNYKVKAIAGNGRL
                     STFNGLDGGVTATELDLLLNPKHWVNIATSVAPDSAGVILQEPSAKQHLAGTQITLTA
                     QRRYGYTFIKWINANNEVLSEEESFVLTLDKDEQVTAVFEKLPLYSLNCSVIGGPQEL
                     IQLCGSDTVIDGVTYYEAGTEVTLSVVDHPLFRLDSWENASTDSVRIITMNENKNLTA
                     RFRLHPFIVAWTFRMPGNSSYSSDYCSDENNIGEFNLRDQQGNTADWLNYSLLEGGYL
                     GRSAVLNGNDLNDRYYYEASFSTKGHSDIVLRSSMAVDSNGYSVQKAEYSTDNGASFE
                     TLGVIDIDSTKSWNNSVLPLPENAWDQDLVHIRWIPDYISEIHGDPQGTDGTAIGDIY
                     VLLQKSDALPRVNKEISLNIWTDGNKQVYLQSNRPVHAVQVFDMTGRCVVRLSGEEIR
                     SVNLSALSRGMYVLAAYNEINEMAVAKIILR"
BASE COUNT         2804 a         2701 c         2904 g         3107 t
ORIGIN      
        1 aatcccaggc tatcctggag attggatacc gaaaaagaaa acagtcttca aaccgcgtac
       61 agaattaagg tggcatccac tcccgaagca ttaaaacgca atcaattggt gtgggacagc
      121 gatgtggttt cgtccgaccg gtcggtgttt gtggagtatc agggaccgca attggtttcg
      181 gggcagaggc tttactggca agttacggta tgggacaatc agggcaatca ggctacttcc
      241 ggtgagacag cttactggga aatgggctta ttgacttccg gcgaatggaa agctgcctgg
      301 atcacccccg gctggaagga agatacatca cagtccaatc cttgtccctt tttcaggaaa
      361 gaatttaatg taaagaaaca agtggccaag gccaggatgt atgcctcggc tcttggttta
      421 tacgagctat acctaaatgg ggagaggctt ggcaatgatt ttttcaatcc gggctggact
      481 acttacagta aacgcattca gtatcaggtg tacgatctta ccgccttgtt gaagcaaggc
      541 cggaacgctc tgggagccat cgttggtgac ggttggtatc gcggtttttt cggctttcag
      601 ggtgaacgta atctttacgg cgataaggcc ggcctgatta ctcagctgga gattaactac
      661 gaagacggct ccaaagaatg gattttgagc gatccgtcct ggaaaagcgg taagggaccc
      721 ctcctcaaat cggaaattta ccatggcgaa ttttacgacg cgaggcttga aatcctcggt
      781 tgggcagagc cgggatttga tgattccaag tgggaaaaag tcaccacact gaatcagtcg
      841 aacgaagtgt tggttccaac cgaaggagag cccgtccgaa ttaccgaaac ccttaaaccc
      901 atcaagaaat ttgtgacgcc ccgcggggag ttggtattcg atttcggaca gaacctggtg
      961 ggttgggtgc gttttcgcct gaagggagaa cggggacaga gtatcgtatt gcaccatgcc
     1021 gaagtgctgg atcaggaagg caacttctat accgacaatc ttagagtggc caaagccgaa
     1081 gacgtctata ttttcaaagg agaaggtgta gaagaatacg aacctcgttt cacttttcac
     1141 ggtttcagat acctgcgtgt cagcgaatat ccgggcgaaa tcggccttga cgatgtagag
     1201 ggccgtgtgg tgcacagcga tatggaaaaa attggggaat ttgaatgttc cgatccttta
     1261 atcaatcgct tacaacaaaa tattcaatgg ggcttgcggg gcaattttct ggatgttccc
     1321 accgattgtc cccagcgcga tgagcgcatg ggctggacgg gcgatgcgca ggcattcgct
     1381 cctactgcct gtttcaacat gaacacggga gctttcttta ccaaatggat gaaagacttg
     1441 cagctcgacc aaaaagcgga cggcaatgta ccctgggtgg ttcccaacgt gctcgaaggc
     1501 ggaggaggca ccggctggtc cgacggatat ggaggtaccg gctgggcaga tgctgccgta
     1561 atcgttccat ggactgttta ccaatcctac ggagacgttc aaatactcga gaaccaatac
     1621 gaatcgatga aagcctgggt ggaatatatg atcaggcatt cgggttcccg ctatattttt
     1681 gattatggtt tccatttcgg ggactggttg tcttttgccg agtattacac ctataattac
     1741 aacgctcccg attacggtta tgccggcgcc cacaccgaaa aagacctgat cgccaccgct
     1801 tatttttatt attccacctc cttgatgagt aagatagctt cgcttctggg ccggaacgaa
     1861 gattccgagc gttatgccgg ccttttgccc aggataaaag aggcttttca ccgagagttt
     1921 attactccaa acggaagatt ggtttcgggg actcaaacag cctatgtatt ggccttaggt
     1981 tttgacctga tacccgaaga ggagcgtgag gcaatggcaa aacgtctggc ggacgacgtc
     2041 cgttattttg gtcacctgac caccggcttt ttgggtactc ccttactttg ccgtgtgctg
     2101 agcgacaacg gctatcccga cctggcctat caattgttgt tcaataaacg ttatccttcc
     2161 tggctttacc ccgttaccat gggagcaacc accatctggg aacgctggga cggcatcaaa
     2221 cccgacggtt cttttcaaac tgtgggaatg aattccttca atcactacgc ttacggtgcg
     2281 gtgggccaat ggctttacag tactgtggcc ggcctgaaca tcgatccttc gaaaccgggt
     2341 tacaaacaca gtatcatcag gccctgtcct accaggcgat tgaattatgc cagagcaagt
     2401 cttcagacta tgtatggact tttgctctcg tcctgggaga taaaagggga tgatataata
     2461 tataagttca gaataccgcc caatactaca gctaccattt atttggaagc ggaaaaaata
     2521 aattattccc agaaagcaca agatttatcc ggcattaaaa aaatggggga taagcagttc
     2581 gtcatagaag cgggttccgg cagctgggaa tttgtagctc ataaagcagt aataaacaat
     2641 taatccaata actttaatta agaagagtat gtcagaagca agtagcaagg atcttgttga
     2701 gttgaaagaa aatcactccc atccggaaaa aattactaaa gaacaatgga aagcctctgc
     2761 gttggcggga atggcttctt acctggatgc cggttcaatc gtggcactgg gggcaggact
     2821 ggccttgttt caagaatacc ttaatttatc aaacagcgct gtgggtttgt tggcagccat
     2881 cggacccaac gccataggct gcgccatcgg ctctatcatt ggcggaaggc tgggcgacaa
     2941 atggggccgt aaacgcattt atcaaataga cttattggtt tatgcttttg gagttttatt
     3001 gattactttc tgcttcaatg ccaccatgtt gtttgtcggc accttcatag taggagtagc
     3061 cgtcggtgcc gacgtaccca cctcactggc ttttgtgggc gaggtggctc ccgataaggc
     3121 tcgcggcaaa ctgctcggct ttacacaggt ggcctggtct ttcggccctg tggtggtgct
     3181 ctggttggct atgtttttgg cagacctggg agttacgggt attcgcattg tttttttgca
     3241 tttatttgtg gtggccctgg ttacctggta tttgaggcgc agcctgtctg aatcggcccg
     3301 ctggactaaa gcggctaaaa ccaaaggcag ccagcttcgc ttacttttga aaggctccaa
     3361 tttaagagga atggcttata cggccactat ttatcttttc tggaacctgg cggcaggtac
     3421 ggcaggtatt tttaaccctt acattattaa gacacttaat gcaggttctt cgcaggctct
     3481 cagcgttggc ctttcgagcg cgggttttat tatttccata atagtaacct ctttggtttt
     3541 tatgcgttat tccgaccgga attttaaaac cagaaaattg ctttggggca tcggagccgt
     3601 gatgcaggtg gtagcttata gtttgttcct ctttttgccc tttaccattc cggttataat
     3661 cgccaatatt ctgatgtttt cttccggtgg tgcactagcc ggcgaagcct tctacaaggt
     3721 gttcagccag gaacttttcc cgactatgct tagaggtacg gcacagggta taacctttgg
     3781 tatcagccgg acgatactcg gggtctggag cttctttgtg ccgattttgg ccaaaaccgg
     3841 tatcggcccg gtggcagccc tactgtcggt cttccttttg atcagcggct ctgtcggctt
     3901 cttctttatg ccggatactt cgggaaaatc cctggagcag attgctaagg aacgggcgtg
     3961 acaggcgctt ttgtgtggtt aaaaacaaaa aacatttcct atgaaacttc tctttcaatc
     4021 catgggaatt ctatccgtct taataatctg tacgacagga tgttccccga ctaaaagcaa
     4081 gcagtgggcg catttacggc aagacacgct gtatattaag gcttctcagt ttagggccgg
     4141 tgaaaatacc attgctttgg gcgattcggc aaacctgatt cattttgtta ttgccgaaaa
     4201 agacagagtg ccttttaatc agtctgttgt aaaacaaacc aacgagcagg cggcttatgg
     4261 accggatgca aaactgcctt attttaccgt gcgttttgct gttccgattc ctcctgcata
     4321 cacccctttc gaggtgggca cacttgcggg attagaacac ggagtgtatc accacaacca
     4381 ttcgccgggg tttgagatta tgcccaacgg cgatgccctg gctgtgtttt tcagttcgcc
     4441 ggccgggaag agcgaaagcg atacggctgc cacctttgtt caggccaggc ttcgtcacgg
     4501 ctcggaagac tgggatatgc ccgaagtctt ttttgataca cagtttggta acgaccaatc
     4561 cggtttgcta tggaacgaca agggcaaaat ttggtttttc ggaggaggca gatatatctc
     4621 tgattatgtt cccttcagaa tagctgtttc gacggacaac ggtgcctcgt ggactttttc
     4681 tgttcctcag ctcgatacgg cagcaacgga ctacacggct cagccggtag taaacgcttt
     4741 ccgtgatccc cagggtggaa tatatatggt ttgcgacgga gacgggagtg aaagtttttt
     4801 gtggtacagc gccgacgagg ggaaaagctg gaaagataaa ggtggaagaa ccggcagtcg
     4861 ccattcgacc atactacccc tcgacgataa aggcacccta cttgcacagg gtggaaaaaa
     4921 cgccgatatc gagggttgga atgtccggaa catttcgcac gactggggcg caagctggga
     4981 agaaggaacg tcttcaccct tcccgccctt aggcactgcc cagcgtcctt ctatgatacg
     5041 tctggcttcg ggttgtttgt tttatgtgac agactcctac atgcacaaaa aaaatatcgc
     5101 tccgccggcc ggctgggaat tcgggaacaa tgccgtagtt gccatttcca aagacaatgg
     5161 agaaacctgg catattaaac cgcttccggt tcaattgccg caacgccatc gtcctccgca
     5221 cggatcgttg ggctattcaa ccgcacgtca ggcacccaat ggtgttattc atattctgac
     5281 cacggccaac tttcctccca ttcactacga attgaacgaa gcctggatat ggtccgatct
     5341 ggctgatatt accccggaat cggatggtgg cgaagtaaag agctttaccg aaacttataa
     5401 agacggccgg ttaaagtcgg cctggagtgc acgcatttgc cccaatggcc ggtatttatt
     5461 gcacggtgag acgaccgatt attacgaaaa cggtgcgatt cagcacgtgg cagtctatga
     5521 aaacggccgc aaatcgggcg aagagcttta ttacgataaa gaggggaagc ttgaatggaa
     5581 atggcaacgc gatttgcata ctaatacagg agtctggact cagtactggc caaacggcaa
     5641 caagaaggta gaatccgtct ggaatatccg gcctcaagcc agagacttga accgtgcctt
     5701 ctttggatac gtcgccgaag ggccgtccac tcattgggat tccgatggaa acctcgtaaa
     5761 agtgtaccaa ttcagcaaag gcctcttaca agaataagcc aaaaaaagtt ttgccggaat
     5821 cgatgttacc ggagttgtct aacatactcc gtaaggaatg ccctagttca aaaagagctc
     5881 gattacagga atttcgaggg gtggtttatt aacaggcagg ctcaacctca agtcgttgtc
     5941 ttccgtgctt ccctgatcga tggctatgaa tccttttgaa gtggatattt taatttcgga
     6001 ggcatcgtgc aggaattgca ggtagttgac tttccctttc attcccttca gggtaatatt
     6061 gcgcagggga taatcgagta aatgaacgta gagcctgttg gtcgtgggat tgtaggtcaa
     6121 taaggtgttc tccggagtct caaaagaatc gggagcctgg gtgcagccgt aaatggagcg
     6181 ggcgttgtat ttcatccagt ctccgatttt tagcagggcg ttttctgccc gatagtcgat
     6241 ggtgcccctg ccggtaggcc ctacgttcaa caaaagattg cctcctttgc ttaccgattc
     6301 tatcaggagc accagcaatt gtttgttgtc tttccaggtt aattcatccc ggtagtagcc
     6361 ccaggatccg ctgaaagttt ggcaggtttc ccagggaatc ttttctccct tgtaggtagg
     6421 ccatttatct accttatgtt gctcgggagt ggcaaagtcc catcctcccc aataatcgct
     6481 caaatccata cggtcgttta ctattatccc cggttgaagt ttgcgaacca gttgtatgag
     6541 ctcaactgaa ccccaatcct cacatccctt accgaattct tcttcgggga aagaaaaatc
     6601 cagaaacaag aggtcaattt taccgtaatt tgtcaacaac tccgtgacct ggtttttgag
     6661 gaactcgcgg taaacagaca tgtcgcggca gcggttgagt cgttcgtaat cttccttgtt
     6721 ttcagggctt tgaggatgtt tgacatctat ggtgtactcg ggatgatgcc aatcgagcag
     6781 ggaataataa aacccgattt tcagcccttc ttcgcggaag gcctcaaccc attcttttag
     6841 agcatctttg ccgtaaggag tgttggtgat tttataatcg ctgaatttag tgtcgaacat
     6901 ggcaaaaccg tcgtggtgtt tcgaggtgag tactgcgtat ttcatgcctg ccgtcttggc
     6961 caatttggcc cattcccggg gctggtacag atcgggattg aaatggtcga cgtatttttg
     7021 atagcttttg tcgtccattt gttcccgttt ttttacccat tcatgacgag cggccagaga
     7081 gtagggcccc caatggataa acaagccaaa gcggtcgttg gtccaccagg caagacgttc
     7141 ttttttttct tccgggcttt cgttccagat ttttttctgt tgcgcattgg cacttaaaaa
     7201 agccatacat aaacaaacga ggattatgat ttttttcatc gcctaaataa attgattgtg
     7261 tcagttttgt tggaaactgt aaatgtaaca aaagtataaa gtgagtcgaa aaactcaaat
     7321 cagttttgaa ccaaaccgtt caaataatgc tccagcatgg tgtagccttc tgcgtttgtt
     7381 gtgttccggt cttcggggtt cgaggggtca agcccgtgtt tggtttccca ttcgtcgggc
     7441 ataccgtcgc gatcgctgtc cacgggagca ggaaggctat tcaacacagg ccatccgccc
     7501 acttccgaag tggacatcac cacgccctta tcataggtgg tgttgccggt gcgggcttcc
     7561 tcaagaattc tcaaatcgac gccatctctt ttggggaaac aagctccggc tcccgctaaa
     7621 acagataaat aggctgcttc ggcctgttcc gtagttatag ccgagaattc atttggttgg
     7681 tctaccatcc attgagtagc gcctttcact ccaaggctgt tgtcgcgtga aatgctttcg
     7741 ttgccctccc acacattacc ttcgatatac caggaacttt ccggtatttc tttgtaaaca
     7801 ccggcttcgt ctatccgggc gaacacatcg atgctgctgt tgggtccggg tttgaagtaa
     7861 ttgttgacaa tgttgatgct gctcggttta ccgtcgaagg tgcggtggcc gaaattgaag
     7921 ataacgttgt tgcggaaatc catacgatgg gtttgatagt tgtggttacc ccctacgctg
     7981 ggattccgtc ccggacaatt ggcgataaga ttatggtgat aggaagtatt tatgccaccc
     8041 agtgttgctg caaatccgtg ttctttgtgg tgcagacctt cggctatgat gcaccactga
     8101 aggcttaagt ttttggaacc atgccatgtg ttgaccactt cgtcggtgcc ccagctaaac
     8161 gaacaatggt caactataac gttttcggcg ttctgaatgg tgatggcgtc gtcttgtttt
     8221 tcggacaggt tgccgcgcct cactcgcaaa tagcgaagaa ttacgtgatc ggcagccagc
     8281 ttgattttat aatttttcag agtgatgccg tcgccgggag ctgtttgtcc ggcaatagtc
     8341 aggtaaggat tgccgatagt cagatcgctt tcaagttcga tagtgcctga aacattgaaa
     8401 ataaccgtac gcggcccttc ctgttcaaca gcctgacgga ggctgccggg gccgctgtcg
     8461 ttcagattgc ttacaaaaag aaccatgcct ccgcgtccgc ccagggcttt agcaccatat
     8521 ccttccgcac ccggaaatgc caatggttga gagcaaacgg gcaaggatag gataaaagaa
     8581 acgactatgg taaataaaaa agatctcata ataagtaact tgaaaattac cttaacaaaa
     8641 taagtccggc ttaatgttag cgactagtac tgaaatttac ctcaaaatga tcttggcaac
     8701 agccatctcg ttgatttcgt tgtaagctgc caatacatac atgcccctgg agagagcgga
     8761 taaatttacc gatcggattt cctcgccgga aagccggaca acacagcgac cggtcatgtc
     8821 aaacacctgg acggcgtgta caggccggtt gctttgtaga tagacctgtt tattcccgtc
     8881 tgtccagata ttcagggaaa tttctttgtt cacccggggc agagcatctg atttttgtag
     8941 caagacataa atatccccaa tagctgtgcc gtccgttcct tgggggtctc catgtatttc
     9001 ggagatataa tcgggtatcc agcggatatg taccagatct tgatcccagg cattttcggg
     9061 taagggcagg acgctgttat tccagctttt tgttgaatca atatcgataa ctcccagcgt
     9121 ttcaaagctt gctccgttgt ctgtggaata ttctgctttt tggacagagt agccgttgga
     9181 atctacggcc atggacgatc gtaatacgat atccgagtgg cctttggtcg aaaagctcgc
     9241 ttcgtaataa tagcgatcgt tcaagtcgtt tccgttcagc acagccgaac ggcccaggta
     9301 accaccctcc aaaagggaat aatttaacca atcggccgta ttcccctgtt gatcacggag
     9361 gttaaattct cctatattgt tttcgtcgct gcaataatcg gagctataag aggaatttcc
     9421 gggcattctg aacgtccagg ctacgataaa agggtgtaaa cgaaaacgtg ccgtgaggtt
     9481 tttattttcg ttcatggtga tgatgcgaac cgagtcggtg gaggcatttt cccaagagtc
     9541 gagacgaaag agcgggtgat caacaacgct caaagtgact tctgtgccgg cttcataata
     9601 agtgacgccg tcgattaccg tatcggaacc acagagttga atcagttcct gtggcccgcc
     9661 aattaccgag caattcaggg aatacaaggg aagcttttcg aaaacagccg tcacttgttc
     9721 gtctttgtcc agcgtaagta caaagctttc ttcttccgaa agaacttcgt tgtttgcgtt
     9781 gatccacttt ataaacgtat agccgtatct gcgttgagcg gtaagtgtta tctgtgtgcc
     9841 cgcaagatgc tgtttcgcag atggctcctg cagaatcact cctgcagaat cgggagctac
     9901 cgaggtggct atatttaccc agtgtttagg attcagcagc aaatccagtt cagtagcggt
     9961 aactccgccg tccagtccgt tgaaggtgct caatcgacca tttccggcga tggctttcac
    10021 tttgtaattg accgttcccc ggtcaacaag atcataataa ctgtttgttt cggcaaagtc
    10081 tatggcggca tcgttggcgt agaccacata acccaccgcg ttttcgacgg ccgtccaact
    10141 tagttttctg ttgctgactg tcagagattt ggggccggag aaagggagga attgcgagac
    10201 cggatcaaaa gcgctcgatg aaacagcccg gtagatggtg ttcattccca gtaaattata
    10261 acggtctttg tcgctcagct gcatactcca cgacactctt ccggaaacgt cggcaagggc
    10321 cgatccgtcg gcattaaggc ttttgtattc ggcaaacgag caattttgtt cgttcccgcc
    10381 ccaggcattc cagccggccc gattgatatg gctgcccagg cggcaattca taaaaatact
    10441 gcccgattct gaagaccagg gacgtcctag ataaacgtcg tttgctccgt ctttggccaa
    10501 gaggtcgcag tcgttaaata taaatctgta gtacaggatt ttaccgttgc tcagtcttgc
    10561 agtatataca atatcttcgg gtgccgtaat gtagccattc cggttgctca cgatcttgca
    10621 ttgataaaaa taacaggtgc cgccggcgta aataaaatcg actcctccct caatggtaca
    10681 ttgatagtaa aaataccgcc ttcctttttt ggtgcggtgt gtatcctgat taccgagaat
    10741 cttacaattc ttcaatacct gtctgtctcc gctttgataa atggcttcgg cctgtgctgc
    10801 cttgctggga tttttgacgg taatgttttc catgtagaaa tcgggggcgg tcaccgtaaa
    10861 ggtggcagcc gtcatgccgt cggctacaat cgtaccctct ctacccgggt aggtgatggt
    10921 tttaccgtgg taatcttccc acacaatcac cacgctgtca acgttctcgc caatcatgct
    10981 gatcactttt cttgagtcct gccagcgatt accgataaat actttctctt cgtaaactcc
    11041 ctttttaaca aaaatggttt tgcggccggc gttttcggga acggtttcga tagccgcatt
    11101 gatgctggta aaatcaccgc tgccgtcttt ggctacaaca atatcatagg gcttggctgt
    11161 ttccacggga atgtcttcgc cgaccaggct gcacaagtag gcatccaaag ccgtatatcc
    11221 gcttttcaga atcaggttgc ggtcgtcggg attcgaagga tccaggccat tggcgagttc
    11281 ccagtcgtcg gccataccgt cgtggtcctc gtcttgtacc tgattgtagg tgttgtaaac
    11341 cggataaccc ccggcatcgg atggtttatt tataataccg caaacccggt gattgttgaa
    11401 ggtaccgtga gcagaagccg tgcaggtggt gacttcgtga ataatacgtc tgtccacggt
    11461 atcgcggggg aaagccccaa cgcccggcag taccacggcc cgggcggcta agattt
//
LOCUS       DAOSGL010000032        11434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4776_length_11434_cov_5.199754, whole genome shotgun sequence.
ACCESSION   DAOSGL010000032 DAOSGL010000000
VERSION     DAOSGL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4776_length_11434_cov_5.199754"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PKY68_01990"
     CDS             <1..1134
                     /locus_tag="PKY68_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOH23843.1"
                     /translation="ENVHSYVNNINTIEGGTHLTGFRRGLTRTLKKYAEDSKMLEKLK
                     IEISGDDFREGLTAVISVKVAEPQFEGQTKTKLGNTDVIGIVDQTVSEALGYYLEEHP
                     KEARIIVDKVILAATARHAARKARELVQRKTPLSGGGLPGKLADCSSRDPQKCEIFLV
                     EGDSAGGTAKQGRNREFQAILPLRGKILNVEKAMPHKVLESEEIRNIYTALGVSIGTE
                     DDSKELNISKLRYHKIIIMTDADVDGSHIDTLIMTFFFRYMKPLIEQGFLYIANPPLY
                     LCKKGKIEEYCWNDKQRQRFIEQHAGGNESAVHTQRYKGLGEMNAEQLASTTMDPEHR
                     ILRQVTIENAAAADHIFSMLMGEDVGPRRNFIEANATYANIDA"
     gene            complement(1140..2300)
                     /locus_tag="PKY68_01995"
     CDS             complement(1140..2300)
                     /locus_tag="PKY68_01995"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOH23844.1"
                     /translation="MITTIVIVLFYLFVPAIVLRLCHLSKMADKIGAVLICYLIGMVF
                     GALVINDERYAIPDAISTIMIPLALPMLMFEVNIRDWSKMAGKAFLSMLAGVFGLVIV
                     VVLGYFLFKESVPEAAKVSGMLAGVYTGGTPNLASIKAALGVPEDLYIAVNTVDMLVS
                     AAYLLFLLSYAKPLLSKILPPYQSKHGVQQIETNSLLQEEPKLALIPWLKKVKTRHIL
                     AAFGVTLLCAAGGAGLGLLVKNPAYQMAVIILSITTLGIIAANLKPVKKIQGSFDTGM
                     YLILIFSVAVASMADFKDISSKLLPLIGLLSFVIFGSLLLHMLTCKLFRIDTDTAMVT
                     SVALVCSPPFVPVVCGAIKNREVMISGITVGVVGYALGNYLGIAIAYFLQGI"
     gene            complement(2311..3117)
                     /locus_tag="PKY68_02000"
     CDS             complement(2311..3117)
                     /locus_tag="PKY68_02000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH23845.1"
                     /translation="MKPIRYLLFCLVLISWQLKAQKQDTIYISTSLGTMKAVFYDDVP
                     RHRSMYTERIKYEAFDGTLFFRVVPNFIIQGGSPDSRNAAPGERVGQGSNQFLLKPEF
                     RPAHFARKGAIAAPRQPDSMNPEKESDCSQFFIVKGRVYTGGYLDSLEMQRNNPLKNA
                     WWAVNYTPHKAEMDSLRKADPRQFNQRLRSYRAEMDSALRSNPQALFFSEEQRAILTT
                     LGGELSLDMQYTFFAEVVEGLEVIDKIAALKTDAYERPLQDVVIHSVRIK"
     gene            complement(3128..3198)
                     /locus_tag="PKY68_02005"
     tRNA            complement(3128..3198)
                     /locus_tag="PKY68_02005"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3164..3166),aa:Gln,seq:ctg)
     gene            complement(3262..4584)
                     /locus_tag="PKY68_02010"
     CDS             complement(3262..4584)
                     /locus_tag="PKY68_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH23846.1"
                     /translation="MNDTEYRRYFQQRLSNYESDEVPEFDQLFSKADLDLVPAKSGGF
                     RLWPVVLSVVSAAAVVALLLILWPAGKDSDYFSYKEYRESFSLPDVSSIEIKSNIQAY
                     DEPVLLATSALPDNSDRHTVRRFETIGVQIPASPVSTGSEGVLSETTADGRQAVDAET
                     QQEQASTGSNQASEMIHQNTYERSVQEAHRLAAENARQRKRIFRPRLGVAINQNNGLL
                     ASVSPNSYQSMAASFANDYQNAYSVLNAASLMRSGEVDPNAWEKPGNLDMPVVEKPNY
                     HFPLNLSLSVSLPLSSRLELQTGLSYSYLSATTEGETNLYNFTLFQELHYLGVPLRIA
                     YRMIDSDRFGMYASLGGSVEKGLVGKQKNTVVTADNRRDVWTGQQSVHGIQPIASAQL
                     GFDYALNPKLQLYLEPGVVYYFDTDQPLSIRTKTPLFFNLGLGLRYRI"
     gene            complement(4565..5131)
                     /locus_tag="PKY68_02015"
     CDS             complement(4565..5131)
                     /locus_tag="PKY68_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH23847.1"
                     /translation="MSGALNTDLLLIEACLKGDAKAQKELYEQYARKMMPICLRYSND
                     YETAKDLLHDGFVRVFTYLGDYKGEGSFEGWMRRIFVNTALEYLRKFKDKPHLMNIED
                     AYMLESNEHSVLEKMTEAEIIACIQELPTMYRSVFNMFVIEGYSHREIGELLKITESS
                     SRVYLTRAKQLMQSMLIKRYGKNERYRI"
     gene            5228..5965
                     /locus_tag="PKY68_02020"
     CDS             5228..5965
                     /locus_tag="PKY68_02020"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOH23848.1"
                     /translation="MGALKWIGGALGWALGGPLGGILGFIFGSIFESVSSGKYAYQQQ
                     DQVQGVRTGEGDFGLSLLVLSAAIMKSDQRVLKSELQYVKNFFRQQFGQEKAEQYILI
                     FRELIKKDYDLVPVCQQIRQAMDYASRLQLLHYLFGLGQSDGRLQEAEIRLIQSIASW
                     LGISVMDYESIRAMFIKDATSAYKILEISPEASVEEIKKAYKRMAVKYHPDKLEHLGE
                     DVQKKATEKLKEVNVAYEQIKKERGFN"
     gene            6087..6515
                     /locus_tag="PKY68_02025"
     CDS             6087..6515
                     /locus_tag="PKY68_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23849.1"
                     /translation="MKRFSLVITMALLFVAGTITAQQQQRTGQRGQFSTTARAEREVE
                     QLKSSLSLTEQQAKDVFKICLKYAQQDSVRMAEMRSSGQEFDREAFMKQREEVTKTKT
                     AELNKVFTPQQQKDYAKQLEEAAQRRMQRQGAAPGQGGNR"
     gene            complement(6762..7619)
                     /locus_tag="PKY68_02030"
     CDS             complement(6762..7619)
                     /locus_tag="PKY68_02030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOH23850.1"
                     /translation="MKNKNKISYIYVFLACLLLELIIVPIRGINLELSGLIAFVAYSL
                     FIYFCCKFFRKLNPLLILGFTFLAWFLLNLPVRLFYWKDALITLWELLMHVLGMITGY
                     LLWVNKRIWKFVTLLVATIVCVVYAANNIKWLDYLSFGKVDSEGEQVLEEDFCLRDLN
                     GSELSLNDFKDKYLVLDFWSSSCGLCIKNFPEVQSFSEKFSNNQRVSFYSVFCVSANR
                     CETRQTGFDIVQKRGFSFPLLFSEDESYKKFGVSCFPTVVIFSPDNKIIFRGRLEIAI
                     KFINKNINK"
     gene            8287..9192
                     /gene="lgt"
                     /locus_tag="PKY68_02035"
     CDS             8287..9192
                     /gene="lgt"
                     /locus_tag="PKY68_02035"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOH23851.1"
                     /translation="MGVLDAVTWTFPPQLFNLFGKEVRWYGLLFALGFYFGLVITSKM
                     FKAEKVPDKWVDKLFIYIIIAAAVGARLGHVFFYAWDYYSQHPWEIIKIWEGGLASHG
                     GAIGIILAVWLYSKRVTKRSLNWTFDRIVVSVALAGALIRLGNLANHEIYGHPTDLPW
                     GFRFITNWYQVRRGADPVFSEPSHPTQIYEALCYLLCFVVLYNLYWKTKLKHREGFLF
                     GVFLIGVFLSRFLIEFVKENQEAFEEGMLLNMGQLLSIPFVIAGIWLIIRAFRRPPVY
                     YETNEKPKKDKTNTLLSNVKSTSVK"
     gene            9189..10169
                     /locus_tag="PKY68_02040"
     CDS             9189..10169
                     /locus_tag="PKY68_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23852.1"
                     /translation="MSWQKVLLFVATLLFLSACSSEARYRVLGKPKHPLVFTRYEQDF
                     FEDGRIEDSVFRDLFSNRIMQFGSVGEARTDSFLELFRLDSTMQKVYTDCQALFPDLE
                     IYQKQLSVAFHRLCHFVPDMPLPSVSTHLSGFSQSVVSAPSILSVSLDKYLGSDYVLY
                     QSLFYDYQRQRMRAEQLVPDCLNGWLRSEFTNASIMADSRLLDFIIYEGKILFVLKLL
                     FPKTEIEYLTGWTKEQADWCAVNEKRMWERILEYEHLYSRDHLVLSKYIGDGPATVYF
                     TRESPSRAVLWTGLKIVEAYMQRHKKLSVLDMMMETDADKILTESLYRPK"
     gene            complement(10201..11118)
                     /gene="metA"
                     /locus_tag="PKY68_02045"
     CDS             complement(10201..11118)
                     /gene="metA"
                     /locus_tag="PKY68_02045"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOH23853.1"
                     /translation="MPLNLPPQLPAIDILKKENIFVMDENRASTQDIRPLRIVVLNLM
                     PIKITTETDLIRLLSNSPLQLEMKLMKIKSHTPKNTPVEHMKAFYETFERLRSEKFDG
                     MIITGAPVEHLDYEEVTYWEEVKEIFDWSRTHVTSTMYICWAAQAGLYHQYGIPKYNL
                     PEKKFGIFKHRANQPEYPLFRGFDDEFYVPHSRYTEIRREDILNKPELTLLAESEEAG
                     VYIVMGRNGREFYLTGHSEYAPDTLHKEYLRDLEKGLKIAVPSNYYTDDDPTKQPLVR
                     WRGHANLLFSNWLNYFVYQETPFDISQVH"
     gene            11171..>11434
                     /locus_tag="PKY68_02050"
     CDS             11171..>11434
                     /locus_tag="PKY68_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOH23854.1"
                     /translation="MAIFAQFLAGVDGKRMNTINKFKLNKVFVPKFYELIKRKQYSKA
                     LFVKDLTAGVIVGVIAIPLAIAFGIASGVSPQQGLITAIIGGLV"
BASE COUNT         3343 a         2549 c         2453 g         3089 t
ORIGIN      
        1 gaaaacgtcc attcctacgt aaacaatatc aataccatcg aaggcggtac acatctgacc
       61 ggttttcgcc gtggccttac acgcactctg aaaaaatacg ccgaagattc caaaatgctc
      121 gaaaagctaa aaattgaaat ttccggtgac gactttagag aagggctgac agcggtaatt
      181 tcggtcaagg tggccgaacc tcaattcgaa ggccagacca aaacaaaatt agggaacacc
      241 gatgtaatcg ggatagtaga tcaaacggtc agtgaggctc ttggatatta cctcgaagaa
      301 catccaaaag aagccagaat tattgtggat aaggtgattc tggctgctac tgcacgccac
      361 gccgcccgta aagccagaga actggttcag cgcaaaacac ccctctccgg aggcggcctt
      421 ccaggcaaac tggccgactg ttccagtcgc gatccccaaa aatgcgagat attcttagtt
      481 gaaggtgatt cagccggcgg cacagccaaa cagggaagaa atcgtgaatt tcaggcgatc
      541 ctgcctttgc gcggtaaaat cctcaatgtg gaaaaagcca tgccccacaa ggtgctcgag
      601 agcgaggaaa tccgcaatat ttatactgct ttaggcgtaa gtatcggcac cgaggacgac
      661 agcaaagaat tgaacatctc caaattacgc taccacaaga tcatcatcat gaccgacgcc
      721 gatgtggacg gcagtcacat cgacactctg ataatgactt tcttcttccg ctacatgaag
      781 ccgctcatcg agcaaggctt cctgtacatt gcaaatcccc cgctctatct ctgcaaaaaa
      841 ggcaaaatag aagaatactg ctggaacgat aagcaacgcc aacgttttat cgaacaacac
      901 gccggaggca acgaaagcgc cgttcacacg cagcgttaca aaggtttggg tgaaatgaat
      961 gcggaacagt tggccagcac cacgatggat ccggagcatc gtattttaag acaagttact
     1021 atagaaaatg ccgcagccgc cgatcacatc ttttcgatgc tgatgggcga agacgtagga
     1081 ccccgtcgca actttatcga agccaacgcc acctacgcca acatcgacgc ttaaaacaat
     1141 cagatacctt gtaaaaaata ggcgatggct atgcccaggt aattccccag ggcatagccg
     1201 actacgccca cggtgattcc ggaaatcatt acttcacggt tttttattgc tccgcatacc
     1261 accggcacaa agggaggtga gcacaccaat gccaccgatg tcaccatagc tgtgtcggta
     1321 tcaatacgaa acaacttgca cgtcaacata tgcaataaca aagaaccaaa aattacgaaa
     1381 ctcagtaaac cgatcagagg caaaagtttc gatgaaatgt ccttaaaatc ggccatactg
     1441 gctacagcaa cactgaaaat caaaatcagg tacatgccgg tgtcaaaact tccctgaatc
     1501 ttcttcaccg gttttaaatt agccgcaata atacccaggg tggtgatgga taatataata
     1561 acggccatct ggtaagccgg attcttgacc agtaatccca aaccggcacc accggccgca
     1621 cagagcaaag tcactccgaa agcagctaaa atatgacggg tttttacttt tttgagccag
     1681 gggatcaagg ccaattttgg ctcttcctgc agtaaagagt tggtttcgat ttgttgtact
     1741 ccgtgttttg attgatacgg aggaagtatt ttgctcagca aaggcttggc ataagaaagc
     1801 aaaaagagca aatatgctgc actgacaagc atatccaccg tattaacggc aatatataaa
     1861 tcttcgggga ctcccagagc ggctttaata gaagccagat tgggagtacc tccggtatag
     1921 actccggcca gcattccgct cacttttgcc gcttccggta cagattcctt aaacaggaaa
     1981 tatccgagta caaccacaat aaccagaccg aagactccag ccaacattga aagaaaggcc
     2041 ttaccggcca ttttgctcca atcgcgaata ttaacttcga acatcaacat gggcagagcc
     2101 aaaggtatca ttatggtgga aatggcatcg ggaatggcat aacgctcatc gttaatgacc
     2161 agagccccaa aaaccatacc tatcaaatag caaatcagca ctgctcctat tttatcggcc
     2221 atcttggaca gatgacacaa acgcaacact atggccggta caaaaagata aaacaggact
     2281 attacaatag ttgtaatcat aaaaccggct ttatttaatg cgaacagaat gaattactac
     2341 gtcttgaagg gggcgttcat aggcatctgt tttgagtgct gctattttat ctatgacttc
     2401 cagaccttcg acaacttcgg caaaaaaagt atactgcata tccaggctga gttctcctcc
     2461 aagcgtagtg aggattgcgc gctgttcttc cgaaaagaaa agtgcctggg gattgctgcg
     2521 caaagcagaa tccatttcgg cacgatagga tctaagacgt tgattgaatt gccgggggtc
     2581 ggctttcctg agactgtcca tttctgcctt atggggcgtg taattaactg cccaccaggc
     2641 attttttaga ggattgttac gctgcatttc caggctatcc aggtaacctc cggtatacac
     2701 cctgccttta actataaaaa attggctgca atcggattcc ttttcgggat tcatactgtc
     2761 cggctgtctt ggagctgcaa tggcaccttt acgggcaaaa tgggctggac gaaattcggg
     2821 tttaagcaaa aactgattac tgccctggcc tacacgttct ccgggagctg cattgcggga
     2881 atcgggagat cccccctgta taataaaatt cggcaccact ctaaaaaaaa gtgtgccgtc
     2941 aaaagcctcg tatttaatac gttctgtgta catagaccta tgccggggta cgtcatcata
     3001 aaaaacagcc ttcatcgtac caaggctggt agagatgtaa atggtatcct gtttttgagc
     3061 cttgagttgc cagctaatca aaaccaggca aaagagcagg tatcgaatgg gcttcatggt
     3121 gaatttgttg ctccgctagg attcgaaccc agacagacag atccagaatc tgtagtgcta
     3181 ccattacaca acggagcatt ataaaaaacg gcgtaaaagt acacaatatt ttttacgctc
     3241 aaaacagaaa aaggggaaaa atcagatacg gtaacgcaga cccaggccta aattgaagaa
     3301 aaggggagtt ttagtacgta tactcagagg ctggtctgta tcgaaataat aaacaactcc
     3361 cggttcaaga tataactgca atttgggatt caaagcataa tcaaagccca actgagcact
     3421 cgctatgggt tgtatgccat gtacggattg ttgccctgtc catacatctc ttctgttatc
     3481 ggccgtaaca acagtgtttt tttgcttgcc gaccaaacct ttctctacgc taccccccag
     3541 cgaggcatac atcccaaaac gatcgctgtc aatcatccgg taggcaattc tcaaaggtac
     3601 tcccaaataa tgaagttcct gaaacagggt aaaattatac agatttgtct ctccctccgt
     3661 cgtagccgat aagtaggaat aacttagtcc tgtctgcaat tccagcctgg aggacaaggg
     3721 cagcgaaaca ctgagggata aattgagcgg gaaatgatag ttgggtttct ccaccacagg
     3781 catgtctaga ttacccggtt tttcccaagc attgggatca acttctccgc tgcgcatgag
     3841 gctggcggca ttcaacacag aataggcgtt ctgataatcg ttggcaaacg aggcagccat
     3901 cgattgataa ctgttggggc tgaccgaagc caacaaaccg ttattttgat taatggcgac
     3961 acccaaacga ggcctgaaga ttctcttacg ctgtcgggca ttttcggccg ccagcctgtg
     4021 cgcctcctga acgcttcgtt cgtaggtgtt ttgatggatc atttcactcg cctggttact
     4081 tccggtcgaa gcctgttctt gctgtgtttc tgcatcaact gcttgtcgcc catcagcggt
     4141 ggtttcagat aaaacaccct cggaaccggt gcttacgggc gacgcgggaa tctgaacacc
     4201 tatggtttcg aaacgtcgta ctgtatgcct gtctgaatta tccggcagag cagaagtcgc
     4261 cagtagtacc ggctcgtcgt aagcctgaat gttactcttg atctctatag aagaaacatc
     4321 gggaagtgaa aaggactccc ggtattcttt gtagctgaaa taatccgagt ctttacccgc
     4381 cggccatagt atcagcagta aagcgacaac ggcggcggca gatacaacgc tgagtacaac
     4441 aggccataac cggaaaccgc ccgattttgc cggtacaaga tctaaatcag ccttggaaaa
     4501 aagctgatca aattccggca cctcgtcgct ttcgtaattc gacaaacgtt gctgaaaata
     4561 gcgcctatat tctgtatcgt tcattttttc catatctctt aatcaacatc gactgcatta
     4621 gttgttttgc tctggtcagg taaactctgg aggaactttc cgtaatcttt agtaattctc
     4681 cgatttctct gtgggaataa ccttctataa caaacatgtt gaacacgctg cggtacatgg
     4741 tcggcaattc ctgaatgcaa gcgattattt cagcctctgt catcttttcc aacacagagt
     4801 gttcattgct ttccaacata taagcatcct caatgttcat caaatgcggt ttatccttga
     4861 attttctcag atattccaat gcagtattga caaagatcct tcgcatccag ccctcgaatg
     4921 agccctcgcc cttataatcg cccaaataag taaatacccg cacaaatccg tcgtgcaata
     4981 ggtctttggc tgtctcataa tcgtttgaat accggagaca tatgggcatc atttttcgtg
     5041 cgtattgctc atacaactcc ttctgtgctt tggcatctcc ttttagacat gcttcaatca
     5101 acaacagatc agtattcaag gcaccggaca tagtatagat gctttttgtt cgaaaatgtt
     5161 acaaagataa ggaataatga atttatgtat ctttgtcgtt tgattttata cttattttcc
     5221 tttacaaatg ggtgcattaa aatggatagg aggagcctta ggctgggctc tgggtggtcc
     5281 cctgggggga atactcggtt ttattttcgg ctcgatattt gaaagtgtca gttccggtaa
     5341 atatgcttac caacaacaag atcaagttca gggagtccgc accggagaag gtgattttgg
     5401 actgagttta ctggttcttt cggctgctat catgaaatcc gatcaacgag tattaaaatc
     5461 tgaacttcag tatgtgaaaa atttcttccg ccaacaattc ggacaagaaa aagccgaaca
     5521 atatattttg atatttcgcg aactgatcaa gaaagactac gatctggtac cggtttgtca
     5581 acaaatcaga caagccatgg attacgcctc acgcctccaa ctcttacatt atttatttgg
     5641 attgggtcaa agcgacggcc ggttacaaga agcagaaatc cggctgattc aaagcattgc
     5701 ctcctggctg ggcatttcgg taatggatta tgagtcgata cgcgctatgt ttattaaaga
     5761 cgccaccagc gcctataaaa tacttgagat cagtcctgaa gccagtgttg aagaaattaa
     5821 gaaagcctat aaaagaatgg ctgtcaaata ccatcccgac aagctggaac atttaggcga
     5881 agacgtacaa aaaaaggcta ccgaaaaact caaagaagtg aatgtagcct acgagcaaat
     5941 caaaaaagaa aggggattta attaagaagt gtttattgat gttattttat cattatttgc
     6001 ttgccgggca ttaaacgatt ccgatcaaat ggtctctaat acccagttta gcgaattcac
     6061 tttatttatt tttaatcctc atttttatga aaaggttttc attagtaatt acgatggctt
     6121 tgctgttcgt ggccggaaca attacagctc agcaacagca aagaaccggt caacgcggtc
     6181 aatttagcac taccgcccgt gccgaaagag aagtagagca attaaaaagc agcctttcac
     6241 tcactgaaca acaagcaaaa gacgtattta agatctgtct taaatatgcc caacaagatt
     6301 ccgtccgtat ggccgagatg agaagcagtg gtcaagaatt tgaccgcgag gcctttatga
     6361 aacaaagaga agaagtaacc aagaccaaga cagctgaatt gaacaaggtg tttacacctc
     6421 agcagcaaaa agactacgcc aaacaactgg aagaggcagc acaaagacgc atgcaacgtc
     6481 agggtgcagc tcccggacaa ggtggcaaca gataaggtgg aggatttttg agtttgatga
     6541 aggtttaatt tttgggttga gaagctgtct cttttgcgta agtggggaga cagcttcttt
     6601 gttctaaaat ttaatcaatg ctgtccttgg tcggattttt ttgcttttcc gccccttata
     6661 aaaaaagctg tcataattat aaaaaaagca acgatccaat cagtcctttt cctgtcaagc
     6721 ttctatgtat agtgttcatc acattatgac acatacatat tttatttatt tatgtttttg
     6781 tttataaatt taattgcaat ctcaagtctg ccgcgaaaaa taattttatt gtctggcgaa
     6841 aaaatcacga cagtaggaaa gcaactgacg ccaaattttt tatagctttc gtcttccgaa
     6901 aacaacaagg gaaaactgaa tcctcttttt tgaacaatat cgaatccggt ttgccgggtt
     6961 tcgcatctgt ttgcggatac acagaaaaca gaataaaatg aaactcgctg attattgcta
     7021 aacttttcag aaaacgactg aacttcggga aagtttttaa tgcacaaacc acaggatgaa
     7081 gaccaaaagt ccaatacaag gtatttatct ttaaaatcat taagcgataa ttcacttcca
     7141 ttcaaatcac gtaaacagaa atcctcctcc aacacttgtt cgccttcact atctaccttg
     7201 ccgaacgaca gataatcaag ccatttgata ttattcgcgg catacacaac acatactatt
     7261 gttgcaacta agagtgtaac aaatttccag attcttttat taacccatag taaatagcct
     7321 gtaatcatcc ctaaaacatg cattaacaat tcccacaaag tgatcagcgc atctttccag
     7381 taaaacaatc gaacaggaag attcaaaaga aaccaggcaa gaaatgtaaa gcctaaaata
     7441 aggagaggat tgagcttcct gaaaaattta caacaaaaat aaatgaataa gctatatgcc
     7501 acgaaagcaa tcaatccgga aagctctaaa ttgataccac gaatggggac aataatcagt
     7561 tccaacagta agcaggcaag gaaaacatag atgtatgata ttttgttttt atttttcatg
     7621 atgatgttat aatatttgta atatggagaa aaagattatt gaatttatct tcaatttttt
     7681 aattccgaac aattctaaat tgtacaacag agagccctca agaaaaaccg aattcttcct
     7741 atctgcaaag gaaccagccg atacatgtgt gaaaaggatg gttgccagag taagtaaaat
     7801 actgtatcgt ttgagcataa tataatatgt gtcttttatg gtttttatag accaaagata
     7861 gtggggcttc ctaaaaaatt atcgcaaaaa taatactgtt ttattaaaca acaataaaga
     7921 gcgttgtctt taataactct tatagggggg catgttaaca catttctatc attttaaact
     7981 cttcgattta atctggaatt tagtgatgtt gatttaattt cgggctgcgc cctcgacgct
     8041 aaacgctact tttcgggctg cgccctcgac gttaaacgct acttttcggg ctgcgccctc
     8101 gacgttaaac gctacttttc aggctgcgcc ctcgacgcta aacgctactt ttcaggctgc
     8161 gccctcgacg ctaaacgcta cttttcgggc tgcgcccggg aagatagcta cgctcggcaa
     8221 tagcaaaatt tattttgcta ttgctctcgc taaacgctat ctttgagcac tcaaaaaaaa
     8281 agaaaaatgg gagttttgga tgctgttacc tggacttttc cgcctcaact tttcaatttg
     8341 tttggtaagg aggtgcgctg gtatggttta ttgtttgctt tgggctttta ttttgggttg
     8401 gtgataacct ccaaaatgtt caaagccgag aaagtgcccg ataaatgggt agataaactc
     8461 tttatttaca ttattattgc cgctgccgtg ggtgctcgcc tggggcatgt tttcttttac
     8521 gcctgggact attattcgca acatccctgg gaaatcatta agatttggga aggcgggctg
     8581 gccagccacg gcggtgcgat tggtatcatt ctggcggttt ggctctacag caaaagagtc
     8641 actaagcgca gtctcaactg gactttcgac cgtattgtag tatcggtggc tttggcaggc
     8701 gccctcataa gactggggaa tctggcaaat cacgaaattt acggtcatcc caccgatttg
     8761 ccctggggct tccgctttat aaccaactgg taccaggtgc gtcgcggagc cgatccggtt
     8821 ttttcggagc cctctcaccc cacccagatt tacgaagctc tttgctacct actctgtttt
     8881 gttgttttat acaaccttta ttggaaaaca aaactcaaac accgggaggg ttttttgttc
     8941 ggcgtatttt tgattggagt attcctgtcc cggtttttga ttgaatttgt caaagaaaat
     9001 caggaagcct tcgaagaggg catgctgttg aatatggggc aactcctgag tatccccttt
     9061 gtgattgccg gcatctggct cataatcagg gcatttcgtc gtcctcccgt ttattatgaa
     9121 acaaacgaaa aacctaaaaa ggacaaaacg aatactcttt tgagtaatgt aaaatcaact
     9181 tcggtaaaat gagctggcaa aaagtcttgc tctttgttgc tactctcttg ttcttgagtg
     9241 cctgcagcag cgaagcgcgc tatcgcgtgc tcgggaaacc caaacatcct ttggtattta
     9301 cacgttacga gcaagacttt ttcgaggacg ggcgcatcga agactctgtt tttagagatc
     9361 ttttctctaa ccgaatcatg caattcggtt ccgtaggtga ggcccgtacc gattccttcc
     9421 tcgagttgtt tcgcctcgac tccaccatgc aaaaagtcta caccgattgc caggctttgt
     9481 tcccggatct cgaaatatac caaaaacaac tctcagtggc ctttcacagg ctgtgtcact
     9541 ttgttccgga catgcctctg ccgtctgtat ccacccattt atcgggtttt agtcaatcgg
     9601 tggtttcggc tccgtccatc ctttcggtca gtctcgacaa atatctgggc agtgactatg
     9661 tattgtacca aagtctgttt tacgattacc aacgccaacg catgagagcg gaacaactgg
     9721 ttcccgattg tctgaacggc tggctacggt ctgaatttac caatgcctct atcatggccg
     9781 acagccgttt actggatttc attatttacg agggcaaaat actgtttgtt ctcaaattgc
     9841 tctttcctaa aacggaaatt gagtacctaa caggctggac caaagaacag gcagactggt
     9901 gtgccgtcaa cgagaaaaga atgtgggagc gcatattgga atacgagcat ctgtacagca
     9961 gagatcacct ggtcttgagt aaatacatag gagacggacc cgctacggta tattttaccc
    10021 gggaatcacc atcgagagcc gtactctgga cagggctgaa aattgtagaa gcctatatgc
    10081 agcgacacaa aaagctcagc gttttagaca tgatgatgga aacggatgca gacaaaatcc
    10141 tgaccgaatc gctttacaga ccgaagtaat aaagatctcg tgctttcctg ccggcagtca
    10201 tcaatgcacc tgactgatat caaagggagt ttcctgatat acaaaatagt tcagccaatt
    10261 actaaacaat aggttggcat gccctctcca acgaaccaga ggttgtttgg tgggatcgtc
    10321 atcggtgtaa taattactgg ggacagctat tttcaggcct ttttccagat ctcggaggta
    10381 ttctttgtgc aaagtatcgg gcgcgtactc cgaatggccg gttagataga attccctgcc
    10441 gttgcgaccc ataacaatat acactccggc ttcttcggat tcggccaaca gagtcaattc
    10501 cggtttgttt aaaatatctt cacggcgaat ttcggtgtaa cggctgtgag gtacgtaaaa
    10561 ttcatcgtca aaacctctga acaagggata ttccggttga ttagcccgat gtttgaaaat
    10621 accgaatttt ttttcgggta aattgtattt gggaattccg tattgatggt acaaaccggc
    10681 ttgcgcagcc caacagatat acatagtcga ggtaacgtga gtacgcgacc aatcgaatat
    10741 ttctttgacc tcttcccaat aagtgacctc ctcataatca agatgctcaa ccggggctcc
    10801 ggtaataatc atcccgtcaa atttttcgct gcgcagtcgc tcaaaggttt cgtaaaaagc
    10861 cttcatatgc tccaccggag tgttttttgg cgtatgactt tttattttca ttaacttcat
    10921 ttccaactgc aaaggagaat tggaaagcag acgaatcaag tcggtttcgg ttgtgatttt
    10981 tataggcatt aaattaagca ccactatcct gagtggacga atatcctggg tagatgctcg
    11041 gttttcgtcc attacgaaga tattttcctt tttcaaaata tctatagcgg gtaattgagg
    11101 aggcagattt aatggcatat tcttaaaatg agaaatttag gccacaaaat tacggcaaaa
    11161 tttcgtctaa atggctatct tcgcacaatt tttggccggg gtggatggaa aaagaatgaa
    11221 tactataaat aaatttaaac tcaataaggt atttgtacct aagttttacg aactgattaa
    11281 aaggaagcaa tactccaagg ccttgtttgt aaaagacctt acggccggtg ttattgtggg
    11341 agtgattgcc atacctttgg ccatcgcttt tggtattgcc agcggagtga gtccgcaaca
    11401 aggccttatt actgccatta ttgggggact ggtt
//
LOCUS       DAOSGL010000033        11415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4786_length_11415_cov_4.236092, whole genome shotgun sequence.
ACCESSION   DAOSGL010000033 DAOSGL010000000
VERSION     DAOSGL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4786_length_11415_cov_4.236092"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="PKY68_02055"
     CDS             <1..104
                     /locus_tag="PKY68_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOH23855.1"
                     /translation="RTVDVHIARLRKKMGAAGEYLQSRPGFGYRWLS"
     gene            132..1544
                     /locus_tag="PKY68_02060"
     CDS             132..1544
                     /locus_tag="PKY68_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH23856.1"
                     /translation="MHYKTRLYLNFGLIALAFMFITILFQLDREKRYKTEEIRSKLAA
                     YSEMIDAYLLQNCDTIEVTHILPEDLRFTIIDSEGSVIFDNVMGHIEDMSNHANRPEI
                     LSARTKGSGYAIRRSVSTQTDYLYYAHRCNTGHYIRLALPYNVKLSDVMKQENLIFYG
                     LAVLFIVVLALLLYKADKFSRVMRSLRRFTSDIEKGTVDYKSFQFPDSDSGIIGKKII
                     SLYEQLEKSKQQTDLEKERNRLIKQEMTNNIAHELKTPVSSIQGYLEILNENGDLEST
                     KRKYFVERAYAQTLRLSALIKDIALITKLEEASNLFPREKVNIGRIAEDVINELQAQL
                     DKAGMKVENRLGADLEISGNYNLVHAIFRNLVENSINYAGKNSSIVVECSKEDTEFYH
                     FKYYDTGYGIKEKYLQKIFDRFVRLEESRDRRTGGTGLGLSVVKHAVLFHQGNIIAVN
                     RPEGGLMFKFCLKKHSAVRI"
     gene            1559..2566
                     /locus_tag="PKY68_02065"
     CDS             1559..2566
                     /locus_tag="PKY68_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23857.1"
                     /translation="MKTRLFLLLWLASSFFFIQAQQANPEFKPNGKAIITVFSDFHTG
                     IGAAGKETGFGLERAYLGYQYAFSESLSGKVVFDMGSSKLAGSALERIAYVKNAMLSW
                     KKGNFSLDAGLIGLKQFSVQEKFWGYRYILKSFLDQYGYGYSADMGLSVQYRINDLLT
                     ADATITNGEGYKKLNTDKYFRYGGGLSITPIKELIFRLYYDRHDKDPDNLTHEKSKQI
                     ASFFAGYQNQAFSLGGEYLRMFHPQFRDDRNQHGYSAYTTIKLPGDFQLFGRWDYSAS
                     NKDYDNPLDVWNPGDQQMAILGVQYAPVKGVKLAPNFRMVAPKNDDALYYITVNLSLS
                     L"
     gene            complement(2582..3619)
                     /gene="galE"
                     /locus_tag="PKY68_02070"
     CDS             complement(2582..3619)
                     /gene="galE"
                     /locus_tag="PKY68_02070"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOH23858.1"
                     /translation="MAASILVTGGTGYIGSHTVVELQQSGHRVIIVDNLSNSNKSVLG
                     GIAKITGIRPDFEQADCTDFKAMDVLFAKYGDIKAVIHFAASKAVGESVEKPLLYYRN
                     NLTSLINLLELMSKYGTEALVFSSSCTVYGQPDVLPVNEDAPIKPALSPYGNTKQISE
                     EIITDSIKAGARYKASILRYFNPIGAHPSAEIGELPLGVPQNLIPYVTQTAAGLREML
                     SVFGNDYNTPDGSCIRDYIHVVDLAKAHVVALDRMLDGKSADKLEIFNLGTGKGLSVL
                     EIVETFQRVTGVKLNYRIVGRRPGDIEQVWADPEKANKVLGWTALETVEETLLSAWRW
                     QQKISSQPEAL"
     gene            complement(3631..4209)
                     /gene="rsxA"
                     /locus_tag="PKY68_02075"
     CDS             complement(3631..4209)
                     /gene="rsxA"
                     /locus_tag="PKY68_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOH23859.1"
                     /translation="MIYITIFIAAVFVNNIVLSQFLGICPFLGVSKKISTALGMSGGV
                     AFVLTLSTLITWLIFRYLLEPFELAFLQTISFILVIAALVQLVEIIIKKISQPLYQAL
                     GVYLPLISTNCAILGVAILVIQKEYNLLESVVFAFSTAIGFGLALVLFAGIREQLSHT
                     KIPKGMQGTPIALLVAGLLAMAFMGFSGIVKL"
     gene            complement(4206..4796)
                     /locus_tag="PKY68_02080"
     CDS             complement(4206..4796)
                     /locus_tag="PKY68_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOH23860.1"
                     /translation="MKNKVGIILNGLIKENPTFVLVLGMCPTLATTTSAINGIGMGLA
                     TAFVLIFSNMVISLIKNLVPDNVRIPSFIVVIATFVTILELVMQAYLPALHESLGLFI
                     PLIVVNCIVLGRAEAFASKNSVLDSALDGIGMGLGFTFALGLLGMIRELLGAGTLFGL
                     QIIPEDYGMLMFILAPGAFVSLAYLIAIVNRIRKVS"
     gene            complement(4793..>5045)
                     /locus_tag="PKY68_02085"
     CDS             complement(4793..>5045)
                     /locus_tag="PKY68_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOH23861.1"
                     /translation="GTQMTYWFKTDKNNQSILNKDPGKDKVWVDKDGGDIDAITAATI
                     TSRAFLDAVDRAYRAYMEHGGTVVDVVTTATIEEKEAQK"
     assembly_gap    5046..5055
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5056..5446)
                     /locus_tag="PKY68_02090"
     CDS             complement(<5056..5446)
                     /locus_tag="PKY68_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOH23862.1"
                     /translation="MKKLESSFKNMVLVLGSISIVAAAALGWINELTSEPIRLAAIAR
                     QEKAIRMVSPEFDNQPVEEQFELIDYEGNSLICFPVKKEGKLLGVAIETFSMKGFNGE
                     VRLMLGLKPDGSINNYEVLKQSETPGLG"
     gene            complement(5443..6414)
                     /locus_tag="PKY68_02095"
     CDS             complement(5443..6414)
                     /locus_tag="PKY68_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOH23863.1"
                     /translation="MENKLYVSMSPHIHGGDSISRKMWDVIIALIPALLVSLYFFGLG
                     SLLVTSVSIASCVLFEYLIARFLLKQESTLRDGSAILTGLLLAFNLPSNLPLWIVVIG
                     ALVAIGMAKMSFGGLGNNIFNPALVGRVFLLISFPQQMTSWPLPKPFNLHYLDAATGA
                     TPLSVMKSSRFDRLPDMLDMFIGNTGGSLGEVAAAALILGGLFLLIRKVITWHIPVSI
                     LLTVYIFTGILRVANPEVYADPLSHLLTGGLMLGAIFMATDYVTSPMVKKGQVIYGIG
                     IGIITVCIRVFGSYPEGVSFAILMMNGVTPLINAYCKPKRFGEVKKK"
     gene            complement(6418..7752)
                     /gene="rsxC"
                     /locus_tag="PKY68_02100"
     CDS             complement(6418..7752)
                     /gene="rsxC"
                     /locus_tag="PKY68_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOH23864.1"
                     /translation="MRTFSMGGIHPPENKISAGKAIEQVPLPAQVAILLNQHSGAPAV
                     PLVQRGDRVKTGQLIAEAAGYISANIHSSVTGKVLKLDEVYDASGYRCQAVIIEVEPA
                     DEWAEEVVLNSTLKRDCTLSPEEIIQKIKEAGVVGMGGASFPSHVKLSPPPGTKPEVL
                     IVNAAECEPYLTSDHQLMLEKGMEIIVGTKITMMALKVNRAVIAIENNKPDAIALLER
                     LAIPYSQIDVMPLAVHYPQGGEKQLIEAVLGRHVPSGALPVVTGAIVLNVGSIFANYE
                     AVQKNKPVIDRVVCVTGKSVKKPGNYLCRIGTSVQDLIDFAGGMPDSTAKVISGGPMM
                     GKALINTQVPVTKGTSGILLMDGNEAHRNEVYNCIRCGDCIEACPMGLEPYLLMSLSE
                     LSEWEAMEKECIMNCIECGCCSYVCPSNRPLLDHLRVGKFKVGAIIRGRKNQ"
     gene            complement(7806..8693)
                     /locus_tag="PKY68_02105"
     CDS             complement(7806..8693)
                     /locus_tag="PKY68_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOH23865.1"
                     /translation="MNVILIAVIVLGGTGIILASVIFLTAKKFWVFEDPRIDEVQNVL
                     PGANCGGCAYPGCRNFAEACVKSESLDGLLCPVGGNDVMKSVASLLGREVVEADPLVA
                     VLRCNGTCANRPKTSRYEGVSSCMAASLLYGGDTACTYGCLGMGDCVAVCQFDALYID
                     KETGLPVVDEAKCTSCGACVRACPRHLFELRKKGPKSRRIFVSCMNKDRGALARKACS
                     VACIACGKCEQVCTFDAIAIKDNLAYIDYNACRLCRKCVEACPTNSIWELNFPPRKKA
                     AEQEVSATKAETGKDATTV"
     gene            complement(8690..9130)
                     /locus_tag="PKY68_02110"
     CDS             complement(8690..9130)
                     /locus_tag="PKY68_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOH23866.1"
                     /translation="MDEIRHYGLVESIDEKGLHVRIDQYSACGACQAKNYCSSSGKKE
                     KIIDILNYQGRYKVGDKVEILGSTAMGYKAVLWAYVVPLCFTVLILVLSLTLLFPGKE
                     APAALLALFSPLAYYLVLYRFRSALKKEFRFKVRSLEDDLSIEL"
     gene            complement(9225..10874)
                     /locus_tag="PKY68_02115"
     CDS             complement(9225..10874)
                     /locus_tag="PKY68_02115"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOH23867.1"
                     /translation="MNAQESIISGNSLHFNAVPSFDNYLQPTPEKDSLPKAPSTEIEI
                     EYSLERALLDSLSAILRKSQGLEYDPDSLWFDSDSLWFDSDTLRFYSDSLGWENALDS
                     LGRILSDTLNLQLSDSLSVDTIPQEELVPKEPELSPLHRYIRDYDIFKDRVYPKPPME
                     LPEDTVLDYPKMSPLFMPLVFSARKREFKLEWREPKRQVIPESWLQTQELGQSIGIPY
                     VKRLARQILLAFEVEQIDKIRYLNTDLPEPERLEVPLEANRPIQWSAPQRVLPRSIEE
                     PPTAPVRYTHWVVKGRLSNQLTQTYVSPNWSSGGVSNMSTLSAFYWVAKYDNKKGIQF
                     DNFIDIKLGFNTVSNDSLRKVNISNDQFQVSSKLGIRAIKNFYYSASVDFTTQFLNNY
                     KPNTYDMKAAFLSPAKLYLGLGMDYKIKNKEKGYSFSALLTPFTYRLNYLQNITAFNP
                     KAFGIEEGKHFGQDMGFKIATTIDMQITEFISWNSYFYFYSDFKYIDSEWKNTFNFNV
                     NHYLSTQLFIHMKMNTKNERPEGEKLIQFKEFLSFGLVYYW"
     gene            11047..>11415
                     /locus_tag="PKY68_02120"
     CDS             11047..>11415
                     /locus_tag="PKY68_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOH23868.1"
                     /translation="MKNTKFIFVTGGVVSSLGKGALSAAMGKLLQARGYRVTIQKFDP
                     YINVDSGTLNPLEHGECYVTCDGHEADLDLGHYERFLNIKTTRANNITTGRIYQTVIN
                     KERRGDYMGKTVQVVPHITDE"
BASE COUNT         3234 a         2745 c         2474 g         2952 t
ORIGIN      
        1 aaagaaccgt agacgtacat attgccagat tacgaaaaaa gatgggtgcg gcaggcgaat
       61 acttgcaaag ccgtccgggt ttcggctatc gctggctttc ctgaatctaa catttaatac
      121 agcccgacat catgcattac aaaaccagat tataccttaa cttcggcctg attgcgctgg
      181 cttttatgtt tatcaccatt ctcttccagc tcgacaggga aaaacgctac aaaaccgagg
      241 aaatacgatc caaactcgct gcctattccg agatgataga cgcatacctg ctgcagaact
      301 gcgataccat agaggtgacc cacatactgc ccgaagattt gcgctttacc atcatcgata
      361 gtgaagggtc ggttattttt gataatgtaa tgggccatat cgaagacatg agcaatcatg
      421 ccaaccggcc tgagatactg tcggcgagga ccaagggttc gggctacgcc atccgccgtt
      481 cggtttcgac ccaaacggat tatctctact atgcccaccg ctgtaataca ggacattaca
      541 ttcgactagc actgccctat aacgtaaaac taagcgatgt catgaaacaa gaaaatctga
      601 ttttctacgg actggcagtc ctctttatag tggtgctggc gcttttgttg tacaaagccg
      661 ataaattcag ccgggtaatg cgttctctca ggcgttttac ttcggatatc gaaaagggta
      721 cggtagatta taaaagtttt caatttcccg attccgattc gggcattatt ggtaaaaaaa
      781 taataagcct ctacgaacaa ctcgaaaaaa gcaagcaaca aaccgatctg gaaaaagagc
      841 gcaaccgctt gataaaacag gaaatgacca acaacatagc tcacgaactc aaaacccctg
      901 tgagcagcat tcagggctat ctggaaattc tgaacgaaaa tggagatttg gagagtacca
      961 aaaggaaata ctttgtggaa cgtgcctacg cccaaacctt gaggctttcg gcattaatca
     1021 aggatattgc tctgattacc aaactcgaag aagcttcaaa cctctttccg agggaaaaag
     1081 tgaacatcgg taggattgcc gaagatgtga ttaacgaact gcaagcccaa ctggacaaag
     1141 ccggtatgaa ggtcgaaaat agattaggtg ccgatcttga aatttcgggc aactacaacc
     1201 tggtacacgc catcttcaga aatctggtcg agaactcgat caactacgcc ggcaaaaaca
     1261 gctcaattgt tgtggaatgc tctaaagaag atactgaatt ttaccacttt aaatactacg
     1321 atacaggcta cggtattaaa gaaaaatacc tgcaaaaaat cttcgaccgc tttgtgcgac
     1381 tcgaagaaag ccgcgatcgc cgtaccggag gcaccggact gggactgtcg gtagtgaagc
     1441 atgctgtgtt gtttcaccag ggcaatataa tagcagtaaa cagaccggaa ggaggactga
     1501 tgtttaaatt ctgcctaaaa aaacattcgg ctgtcaggat ttagataata aatgaaccat
     1561 gaaaaccaga ttattcttat tgctttggct agcaagctct ttctttttta ttcaggcgca
     1621 acaagcgaat cccgaattta aacccaatgg aaaagccatt attaccgttt tcagtgattt
     1681 ccatacaggc ataggtgctg ccggaaaaga aaccggcttt ggactggaaa gggcttacct
     1741 ggggtatcaa tacgctttca gtgagagttt gtcgggtaag gtggtttttg atatgggcag
     1801 cagtaaattg gccggatcgg ctctggaacg cattgcttac gtcaaaaacg ccatgcttag
     1861 ctggaaaaaa gggaacttca gtctggatgc cggacttatc ggtttgaagc agttcagtgt
     1921 tcaggaaaaa ttctggggct atcgctatat cttaaaatct tttctggatc aatacggtta
     1981 cggatacagt gccgatatgg ggctgagcgt tcaataccgc atcaacgatc tactgacggc
     2041 agatgccacc atcaccaacg gagagggcta taaaaaactc aatacagata agtatttccg
     2101 ctacggaggc ggcctcagca ttactcccat taaagagttg atattcaggc tttactacga
     2161 cagacacgac aaagacccgg ataatctcac ccacgaaaaa agcaaacaaa tcgcctcctt
     2221 ttttgccggt tatcaaaacc aagctttctc tctgggcggc gaatatctcc gtatgtttca
     2281 cccgcaattc agagacgaca gaaatcaaca cggttactcc gcctacacta cgatcaaatt
     2341 acctggagat tttcagctct tcggccgttg ggactattca gcctccaaca aagattacga
     2401 caatcccttg gatgtctgga atcccggaga tcaacaaatg gccatactgg gcgttcaata
     2461 tgcaccggtc aaaggtgtta aactcgcacc caacttccgg atggtagcac ccaaaaacga
     2521 cgatgctctc tactatatta cggtaaattt atccctgtcg ttgtaaatac ccaactttac
     2581 attacagggc ttccggttgg ctactgattt tttgttgcca gcgccaggcc gacaagaggg
     2641 tctcttctac cgtctcgagg gcagtccaac ccaatacttt attggccttt tcgggatcgg
     2701 cccaaacttg ctcaatatct ccgggacggc gtcctacaat ccggtaattg agcttgacac
     2761 cggtgacccg ttgaaaagtt tcaacaatct cgagcaccga cagtcccttg cctgtgccca
     2821 ggttgaatat ttccagctta tcggcagatt tgccgtccag catcctgtcc aaagcaacga
     2881 catgcgcctt ggccagatcg accacatgga tataatccct gatacaggat ccgtcgggcg
     2941 tgttgtaatc gttgccaaat acgctgagca tctcgcgcag accggcagcc gtctgggtaa
     3001 cataggggat caggttttgg ggcactccca ggggtaactc tccgatttct gccgaaggat
     3061 gagcgcctat gggattgaaa tagcgtaaaa tactggcttt atacctagct ccggctttga
     3121 tgctgtctgt aataatctcc tcagaaatct gcttggtatt gccataaggc gacagcgcag
     3181 gcttaatggg agcatcctcg ttgacgggca atacatcggg ctggccataa actgtgcagg
     3241 aagaggaaaa aaccagcgct tccgtcccgt atttagacat cagctccagc aaatttatca
     3301 gtgatgtcaa attgttgcgg taataaagca agggcttttc gaccgattcg cctacggctt
     3361 tgctggccgc aaaatgtatg acggccttaa tgtctccgta cttggcaaac aaaacatcca
     3421 tcgctttgaa atcggtacaa tcggcctgtt caaaatcggg cctgatgccg gtgatctttg
     3481 caatgcctcc gagcacagat ttatttgaat tggaaagatt atcgacaatt attacccggt
     3541 gacccgattg ctgcagttca actacggtat gagaacctat gtagcctgtg ccgcctgtca
     3601 caagaataga tgctgccata ttttaataat ttataattta actatacccg aaaatcccat
     3661 aaaggccatg gccagcaaac cggccaccag caaagctatc ggtgtgccct gcataccctt
     3721 gggtattttg gtgtggctga gttgttcacg aatgcccgca aacaagacca gagccagccc
     3781 aaaacctatg gccgtcgaaa aagcaaaaac caccgattcc aataagttgt attccttttg
     3841 aattacgagg atagctacac cgagaatggc gcaattagtg gaaataagcg gcaggtacac
     3901 gcccaaggcc tgatagagtg gctgagagat cttctttatt ataatttcga ccaattgaac
     3961 caaggctgct ataaccaaaa taaaactgat tgtttgcaga aaagccaatt caaaaggttc
     4021 cagcaaatag cgaaagatca accaggtgat aagagtcgac agagtaagca caaaagccac
     4081 acctccggac attcccaaag ccgtgctgat cttttttgat actcccaaaa aaggacaaat
     4141 ccctaaaaac tgcgacagca cgatgttgtt gacaaacacg gctgcaatga agatagtgat
     4201 ataaatcatg atacttttct tatgcggttg acgattgcta tcaaataagc cagactgaca
     4261 aaggctccgg gggccagaat aaacatgagc atgccgtaat cttcagggat gatctgtaaa
     4321 ccaaataagg ttcctgctcc cagcaattca cgtatcatac ccagcaaacc cagggcaaaa
     4381 gtaaaaccca agcccatacc tataccgtcg agtgccgaat caagcaccga gtttttggag
     4441 gcgaaggcct ccgcccggcc aagcacaata cagttgacta cgattagggg aatgaaaagc
     4501 cccagactct cgtgcaatgc tggtaaatat gcctgcatca ccaactcaag aatggttaca
     4561 aaagtggcta tcactacaat gaacgaaggt atacgaacgt tgtcgggtac caggttttta
     4621 atgagcgaaa tcaccatatt ggaaaaaatc aaaacaaatg cagtagccag gcccatgcct
     4681 atgccgttaa tggcggaagt ggtggtggcc agggtcgggc acatgcccaa tacaagtaca
     4741 aaagtagggt tctctttgat caacccgttc aaaattatac caactttatt cttcattttt
     4801 gcgcctcctt ttcttcgata gttgctgttg taactacatc taccacggtg cctccatgct
     4861 ccatataagc tcggtacgcc cggtctacag catccagaaa ggctctggaa gtaatggtgg
     4921 cagcggtgat ggcatcaata tctccaccat ctttgtccac ccaaacttta tcctttcccg
     4981 gatctttatt gagtatactt tggttgtttt tgtcggtctt gaaccaatag gtcatttgtg
     5041 tacccnnnnn nnnnntaccc aggccaggcg tttcgctctg tttcaagacc tcatagttat
     5101 taatactgcc atcgggctta agacccaaca tcagccggac ttcaccgttg aaacctttca
     5161 tagaaaaagt ttcaatagcc acccccagaa gttttccctc ttttttaact ggaaaacaga
     5221 tcaggctgtt gccttcgtaa tcgatcaact cgaattgttc ctctaccggc tggttgtcaa
     5281 actctggcga caccatgcga atagccttct cctgtctggc tatggcggcc aatcgtatcg
     5341 gctcggaagt gagttcgttt atccatccca gggcagcggc agccactata gagatactgc
     5401 ccagcaccag gaccatgttt ttgaatgaag attccagttt tttcatttct tttttacctc
     5461 cccaaaacgt ttaggtttac aataggcatt tatcagaggc gttaccccgt tcatcatcaa
     5521 aatggcaaaa gagactccct cgggatagga gccgaaaacc cttatgcaca ctgtgataat
     5581 tcctatacct atcccatata ttacctggcc tttttttacc ataggtgaag tgacgtaatc
     5641 ggtagccata aaaatagctc ccagcatcag gccacccgta agcaaatggc tgagcggatc
     5701 tgcataaact tcgggattgg ccaccctgag tatgcccgta aaaatataga ctgtcagcaa
     5761 tatcgatacc ggaatatgcc aggtgatcac ttttcggatc aacaaaaaga ggcctcccag
     5821 aatcagcgct gccgcagcaa cttcgcccag actgcctccg gtattgccga tgaacatatc
     5881 cagcatatcg ggtaaacgat caaaacgact cgatttcata accgacaagg gagtagctcc
     5941 cgtagcggca tccagataat gaagattaaa cggtttgggc aaaggccacg aggtcatttg
     6001 ctggggaaaa gaaatcaaca gaaagaccct gcccaccaag gccgggttaa agatattatt
     6061 acccaggccg ccaaacgaca tcttagccat gccaatggcc accaaagcgc caataaccac
     6121 tatccacaaa gggagattgg atggcaaatt gaaagccagc agtaaacctg tcaatatagc
     6181 tgaaccgtcg cgcagagtgc tttcttgttt gagcaaaaaa cgtgcaatca ggtattcgaa
     6241 cagcacacaa gaagcaatcg aaacagaagt taccagcagg gaacccagcc cgaagaaata
     6301 aagcgaaacc agcaatgcag gtatcaaggc aatgatgaca tcccacattt ttctgcttat
     6361 gctatcacca ccgtgaatat ggggtgacat agatacatag agtttatttt ccatagctta
     6421 ttggtttttt cttcctctga ttatggctcc caccttgaat ttacctaccc ttaaatgatc
     6481 aagcaacggc cggttcgaag gacaaacata ggaacaacaa ccacattcaa tacaattcat
     6541 aatgcattct ttttccatag cttcccattc ggacaattcc gaaagactca tcaacaaata
     6601 gggttccaga cccataggac aagcctcgat acaatctcca cagcgtatgc agttgtatac
     6661 ctcgttacgg tgggcttcat tgccatccat gagcaaaata cccgaagtcc ctttggtgac
     6721 gggaacttgc gtatttatca aagctttgcc catcatgggg ccgccggaaa ttactttggc
     6781 tgtggaatcc ggcataccgc cggcaaaatc aatcagatct tgtacggaag ttcctatacg
     6841 gcaaagatag ttgcccggct tcttaacgct tttgcccgtt acgcagacca cccggtcgat
     6901 gacaggtttg tttttttgca ctgcctcata gttggcaaaa atactgccaa cattcaatac
     6961 tatggcgccg gtaaccaccg gtaaagctcc cgaaggcacg tgacggccaa gaacagcttc
     7021 aataagttgt ttttctccac cctggggata atgaactgcc agaggcatca catctatttg
     7081 agaataagga atagccaatc gttcgagtaa ggcaatggcg tcgggtttat tgttttcaat
     7141 ggcaattacc gcccggttca ccttgagagc catcatcgtt attttggttc cgacaatgat
     7201 ttccatgcct ttttccaaca ttagctgatg gtcggaggta agataaggtt cacactccgc
     7261 agcgttcact atcagcacct cgggcttggt acccggagga gggctcagct tgacatgact
     7321 gggaaaactg gcacctccca tacccactac gcccgcttcc ttaatttttt gaataatctc
     7381 ttcggggctc agtgtacagt ctcttttcag ggtggaattc aaaacgactt cctcggccca
     7441 ttcatcggca ggttccactt cgataataac tgcctgacaa cggtagccgc tcgcatcgta
     7501 gacttcatcg agtttcaata ctttacccgt caccgaagag tgaatgttag ccgaaatata
     7561 tccggccgcc tcggcaatca gctgtcctgt tttgactctg tccccccttt gaaccaaggg
     7621 aacggcaggt gcacctgaat gctggttcag caatattgcc acctgcgccg gcaggggcac
     7681 ttgctctatg gcctttccgg ccgagatttt attttcggga ggatgtatcc cccccatcga
     7741 aaaagttctc attgacatat attttcggag tcttcctcta atttctcccg gtattctata
     7801 gttaattata ctgttgttgc gtcttttccc gtttcggctt ttgttgccga aacttcttgt
     7861 tcggctgctt tcttcctggg cggaaaattc aattcccata tagaattggt cggacaagcc
     7921 tccacgcatt tgcgacacaa gcgacaggca ttgtagtcga tgtaggccag gttatctttt
     7981 atggcgattg catcgaaggt acacacctgt tcacattttc cacaagctat acaagccacg
     8041 gaacaagctt tacgcgccag cgcccctctg tccttgttca tacaggaaac gaagattcgt
     8101 ctcgacttgg gacctttttt gcgtaactca aacagatgtc tgggacaggc cctaacgcag
     8161 gctccgcaag atgtacattt agcttcgtcc acaaccggca aaccggtttc cttatctatg
     8221 tacaaagcat caaactggca tacggccacg caatcgccca tacccaggca accataggtg
     8281 caggcagtgt ctcctccgta tagcagggag gcagccatac agcttgaaac tccttcgtag
     8341 cgactggttt tgggtcggtt ggcacaagtc ccgttgcatc gtaaaacagc caccaaagga
     8401 tcggcctcca ccacttcccg acccaggaga gaggccaccg acttcattac atcgttacct
     8461 cctaccggac aaagaagtcc gtcaaggctt tcgcttttga cgcaggcttc ggcaaaattc
     8521 cggcaaccgg gataagcaca acctccgcag ttggctccgg gcaaaacatt ttgtacttca
     8581 tcgattctgg gatcttcgaa aacccagaat tttttggctg tcaaaaatat gacagaggcc
     8641 agtataatac cggtacctcc caaaacaata acagcgatca atataacatt catagctcaa
     8701 tacttaagtc gtcttccaac gatcgaacct taaatctgaa ttcctttttt aaagcagaac
     8761 gaaaacggta caacaccaaa taataggcca aggggctgaa cagggctagc agggctgccg
     8821 gcgcctcttt tccgggaaat aataaagtca gactcaacac caggatcaac acggtaaaac
     8881 agagaggaac cacataagcc cagagcacgg ctttgtagcc catggcagtc gagcccagta
     8941 tttctacctt atcccccacc ttgtatcggc cctgataatt aagaatgtcg atgatttttt
     9001 cttttttccc ggatgaagag caataatttt tcgcctgaca agctccgcag gccgaatact
     9061 ggtctattcg aacatgcagg cctttttcat caatactttc caccaatccg taatggcgaa
     9121 tttcatccat gatcttcctt ttgcttcgtc ctgtaataaa acgaggcaaa gatataacaa
     9181 atctgcaaag gttgtcggtt aatatttgaa tttatatttt gatgttacca ataatagacc
     9241 aggccaaaac taaggaactc tttgaattgt atgagttttt caccttcggg cctttcgttt
     9301 ttggtgttca ttttcatatg aataaacaat tgagtcgaga gataatgatt cacgttaaag
     9361 ttgaaggtgt ttttccactc tgaatctatg tatttaaaat cggaatagaa ataaaaataa
     9421 gaattccagc taataaattc cgtgatttgc atatcgatgg tggtcgcaat cttgaaaccc
     9481 atatcctgcc cgaaatgttt tccttcctct ataccaaagg ctttgggatt gaaagcggtt
     9541 atgttttgca agtaattcag acggtaagta aaaggggtca gcaaggcaga aaaggaataa
     9601 cccttttctt tgtttttgat cttgtagtcc atacccaaac ccagatacag tttagccggc
     9661 gacaaaaagg ccgctttcat gtcgtaagta ttgggcttgt aattgttcaa aaattgagtg
     9721 gtaaaatcga ccgatgccga gtagtaaaaa ttcttgattg ctcttatacc caatttactg
     9781 gaaacctgga actgatcgtt gctgatattc accttacgca ggctgtcgtt ggatacagta
     9841 ttgaatccca atttgatatc aataaaatta tcgaactgta tacctttttt gttgtcgtat
     9901 ttggctaccc agtagaaggc cgacaaggta gacatattgc tcacacctcc gctggaccag
     9961 ttgggggata cgtacgtctg tgtcaactgg ttggataaac ggcccttgac aacccagtga
    10021 gtataacgta cgggagcggt gggaggctct tcaatactcc ggggaagaac tctctggggt
    10081 gcactccatt gtatggggcg attagcttcc agaggcactt ccagcctttc gggttccggt
    10141 aaatccgtat tcaggtagcg gatcttatcg atttgttcca cttcgaaggc aagcaggatt
    10201 tgtctggcaa ggcgcttgac ataagggatt ccgatgcttt gccccaattc ttgcgtttgg
    10261 agccaactct cgggaatgac ttgccttttg ggttctctcc attccagttt aaattctctt
    10321 tttctggcgc taaagaccaa aggcataaac aaagggctca tcttcgggta atccaacacg
    10381 gtatcttccg gtaattccat ggggggcttg ggatacacgc ggtctttgaa aatatcgtag
    10441 tctcttatgt aacggtgtaa aggcgatagt tcaggttctt tgggaacaag ctcctcttgg
    10501 ggtatggtat cgaccgacaa actatcggaa agctgaagat tcagcgtgtc gctcaagatt
    10561 ctcccaaggc tgtcgagtgc attttcccat ccaagactgt cggaataaaa tctaagagta
    10621 tctgagtcaa accaaagact gtctgagtca aaccaaagac tatcagggtc gtattccagt
    10681 ccctggcttt tccttaggat agccgacaag ctgtccagca aggcgcgctc gagcgaatat
    10741 tcaatttcga tttcggtaga aggtgctttc ggcaaagaat ctttttccgg agtcggctgc
    10801 agataattat caaaactcgg aacagcatta aaatgcaatg aatttccgga tatgatactt
    10861 tcttgggcat tcattggtaa tatttgcaaa acaaaaagca atacaaaccc accaattatc
    10921 ctgtttagca tactttgaga ttttctgcga aagtaagact ttctttttaa aacttttttc
    10981 gtacttttgc gcttttagat ttttttagta acgcagaacg taaagttaat ataactactt
    11041 gtgattgtga aaaataccaa atttattttc gtcaccggag gtgtagtttc ttctttagga
    11101 aaaggagcct tgtcggcagc tatggggaag ctcctgcaag caagaggcta cagagtaacc
    11161 attcaaaagt ttgatcctta catcaatgtt gactcgggca ccctcaatcc gctggaacat
    11221 ggagagtgtt acgtaacctg cgacggtcac gaagccgatc tcgacctggg ccattatgag
    11281 cgtttcctca acattaaaac cactcgtgcc aacaacatca ctacgggacg tatttatcag
    11341 acagtcatca acaaagaaag aagaggcgat tacatgggca aaaccgtgca ggtagttcct
    11401 catataaccg acgaa
//
LOCUS       DAOSGL010000034        11296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4856_length_11296_cov_4.611511, whole genome shotgun sequence.
ACCESSION   DAOSGL010000034 DAOSGL010000000
VERSION     DAOSGL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4856_length_11296_cov_4.611511"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3076
                     /gene="purL"
                     /locus_tag="PKY68_02125"
                     /gene_synonym="purI"
     CDS             <1..3076
                     /gene="purL"
                     /locus_tag="PKY68_02125"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOH23869.1"
                     /translation="AFNQGPRIEQFAPASGDKADFFETRDIESVISLKAETHNFPTTV
                     EPFNGASTGTGGEIRDRLGGGKASLPIAGTAVYMTAYTRNDKNRSWEQALQARPWLYQ
                     TPEQILLKASNGASDFGNKFGQPLICGSLLTFEHEENQKKYAYDKVIMLAGGVGYANK
                     KNALKETPHAGQKIVVLGGDNYRIGMGGGAVSSVDTGKFSNAIELNAVQRANPEMQKR
                     VANVIRTLAESDQNPIVSIHDHGAGGHLNCLSELVESTGGLIEMDKLPIGDPTLSAKE
                     IIGNESQERMGLLVNEEDLDLIRRIADRERAPMYVVGETTGDMRLVFKEKNGDRPIDL
                     QLDHMFGKAPRTVITDQTVDESFSALNYDPAKLNEYIQRVLQLDAVACKDWLTNKVDR
                     SVTGKVACQQCAGEIQLPVCNLGAVALDYRGKSGIATSIGHAPQAAIIDPEAGSVLAI
                     AEALTNIVWAPLSGNLSAVSLSANWMWPCKNPGEDARLYKAVQAASDFSIALGINIPT
                     GKDSLSMTQQYDKEKVFSPGTVIISAGAEVSDIRKIVTPVIKPQNDYPLYYIDFSEDL
                     PKLGGSALAQSLNKLGNEVPRVKNAAYFRAAFNTVQSMLEKGLITAGHDISAGGLITA
                     LLEMCFPNANGGLDIDLNSLAGLHESNNDLIHLLFAENPSVLIQVKNPEKTEEYLTQA
                     GIRFCRLGNYIPERKLNIQLEDKRIQQDIDALRDIWYRPSYLFDRKQSGEKAALERYS
                     NYKKQPLEFKFDKNFKGSFLQYGISPDRKGRSGIKAAIIREKGVNGDREMAYCLHLAG
                     FDVKDVHMTDLMSGRETLEDINMLVFVGGFSNSDVLGPAKGWSGGFLFNPTAKKSLDA
                     FYARPDTLSLGVCNGCQCMIALDLIYPEHKDKPRMMRNSSQKFESGFVSAIIPDNHSV
                     MFGSLSGSKLGIWVAHGEGRFDLPYTEDKYHIVAKFNYEAYPGNPNGSKFSTVAICST
                     NGRHLAMMPHFERAIFPWQWAHYPADRSKTDEVTPWIEAFVNARKWIEKKLV"
     gene            3052..3621
                     /locus_tag="PKY68_02130"
     CDS             3052..3621
                     /locus_tag="PKY68_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOH23870.1"
                     /translation="MDRKETGIKAGLSELFFTFLKLGAFTIGGGYAMIPLIEKELIDK
                     KGWFDREEFLELLALAQSAPGIIAMNIAVSVGYKARGKSGMYLASLGAVLPSFLIILL
                     IASVFTSFRDNPVVESIFKGVRPAVVALIAAPIFRLAKSAHITLKTLFIPIGTVILVG
                     FLGVNPVWIILLGAIGGIVWFSIAKRKQL"
     gene            3618..4142
                     /locus_tag="PKY68_02135"
     CDS             3618..4142
                     /locus_tag="PKY68_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOH23871.1"
                     /translation="MIYWQLFITYLKIGFFNFGGGYAMLSLIEHEMVLKHQWVSHAEF
                     TDMVAISQVTPGPIGINMATYVGYTVTGNVWGSMLATFAVSLPSLVVMYAIVRFIQAF
                     RQNKWISAAFDGIKPVTVGLVGSAAILLMNEQNFSDYISLIIFGCSFLLSWAFKVHPI
                     LLIVLAGLTGLLVY"
     gene            complement(4143..5699)
                     /locus_tag="PKY68_02140"
     CDS             complement(4143..5699)
                     /locus_tag="PKY68_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH23872.1"
                     /translation="MARRLLICFALCLILQQAATQNRQSPQTPLPEPEVFFSRRQAEN
                     LPPKREFRGAWLHTVAQTKYAAMSERGMKQYFSRLLDLLHEAGINTVIFQIRPQADAW
                     YNSSLEPWSEFITGRVGRNPGWDPLAYMVDECHRRNMDIHAWINPYRVKLTARESAFR
                     ETFIRKNRNWIVRYGSSLWFDPGIPDCREHIVKVVTEIVQNYDIDGIHMDDYFYPYPI
                     AGLNFPDDASFRNYGKSQGYNSSQKDDWRRNNVNTLVRELHETIRNTKPWVRFGISPF
                     GIYRNIKQDPQGSETDGLSNYDDLYADALLWMKEGWIDYCVPQLYWEIGHSRADYQTL
                     AEWWGKLNFASSIYVGQDIVRTMNPGQLQDKMTLVRNNDGLKGNCFWPAYELENNTGY
                     IVDSLVMNYHYYPSLPPVDLLAEHRAPEPVYELDLRDDYKIGKHLSWKTIRPRSEIER
                     PAYFVIYRFLKGYEPENIEDPRNIIGITQNHHFVLPSHPYSGDWLYVVTALSRVHSES
                     APEGIVLRVY"
     gene            complement(5704..6642)
                     /locus_tag="PKY68_02145"
     CDS             complement(5704..6642)
                     /locus_tag="PKY68_02145"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOH23873.1"
                     /translation="MDCFHVHILGCGSALPTKRHYPTSQIVHLRGKLFMIDCGEGTQL
                     QFRQTGLNYNRIGDIFISHLHGDHCFGLLGIISTMGLLGRNAPLRIHARPELEGLMQP
                     QLDYFCRDLPFQVEFVSFDPGSHSLVYEDRSLEVYCFPLRHRVKSAGFLFREKPRQRH
                     MIKEMIDFYGIPLKEIPAIKGGADFVDSEGRLIANDRLTRPPKAALSYAFCSDTAYDE
                     NIIKYIEGVNLLYHEATFGDDLEELAEATLHSTASQAARIAQKAGVGKLIIGHYSARY
                     TNEEILLQQAQAIFPNTELADEGKCFDVAALRETKN"
     gene            complement(6658..8064)
                     /gene="asnS"
                     /locus_tag="PKY68_02150"
     CDS             complement(6658..8064)
                     /gene="asnS"
                     /locus_tag="PKY68_02150"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOH23874.1"
                     /translation="MNKLQRTKIVDLLQSEDFGSLVQVKGWIRTRRGNKQVGFIALND
                     GSTIHNIQIVVELASFDEELLKQLTTGACIRVDGILSESPAQGQKAEIQAQEIEVYGV
                     ADPNTYPLQKKGHTLEFLREIAHLRPRTNTFGAIFRIRHEMAQAIHRFFYDNGFYYFH
                     SPIITSSDCEGAGQMFQVTTLNLNNLKKNDKAEIDYSEDFFGKHTSLTVSGQLEAELA
                     AMALGAVYTFGPTFRAENSNTPRHLAEFWMVEPEVAFNEIQDNMDLAERFIKYCIQWA
                     LDRCQDDLAFLSKMYDDDLFNRLKFVVDNQFVRLSYTEGIEILEAAVEQGHKFEFPIY
                     WGADLASEHERYLVEEHFKKPVILTDYPKEIKSFYMKQNDDGKTVRAMDVLFPKIGEI
                     IGGSQREENYDKLLSRAKEMGVPDKDIYWYLDTRRYGTAPHSGFGLGFERLLLFVTGM
                     ANIRDVIPFPRTPRNADF"
     gene            complement(8080..9654)
                     /locus_tag="PKY68_02155"
     CDS             complement(8080..9654)
                     /locus_tag="PKY68_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOH23875.1"
                     /translation="MTTNNENRTAKPDEGASVNREEQNKKTSSDQSSHDHYHRRSFDG
                     EDSYDRPRRSFDSESSQDRPRRSYGSEAYQDRPRRSYGDRGNSFEHYGSDRPRRNFSS
                     EDSQDRPRRPFSSEGSQDRPRRPFDSEGSQDRPRRPYGSEGYQDRPRRSYVDRGNSFE
                     HYGSDRPRRSFGSEGSQDRPRRSFGSEGSQDRPRRSYGSEAYQDRPRRSYGDRGNSFE
                     HYGSDRPRRSFSNEGYRDRPRPNFNEPSEKLRSDGRPKRPRIKKSSSDGPSYFKETSI
                     PKYAPDLDPDTPIRLNKFLSNAGICSRREADEFIQAGVVKVNGEVVTELGVKIKPTDE
                     VLFHDQPVSIEHKVYVLLNKPKDCVTTSDDPEERLTVMDLVKNACRERIYPVGRLDRN
                     TTGVLLLTNDGELASKLSHPKYEKRKIYQATLDRDLTEEDMKKVAEGIVLDGEKIAVD
                     EISFVSPDQRNVIGIEIHSGQNRVVRRIFEQVGYKVYKLDRVYFAGLTKKNLPRGRWR
                     YLSDREVSLLKMGAFV"
     gene            complement(9714..11057)
                     /gene="purB"
                     /locus_tag="PKY68_02160"
     CDS             complement(9714..11057)
                     /gene="purB"
                     /locus_tag="PKY68_02160"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOH23876.1"
                     /translation="MDLTELTAISPVDGRYRSKASGLADYFSELALMRYRIQVEVEYF
                     IALCRLPLEQLKQVPHEKFELLRKIYKDFNAGDAQRIKEIEQVTNHDVKAVEYFIKEG
                     FDKLGLHPYKEFIHFGLTSQDINNTAVPLSIKEAVEEQYYPLIEGLCGRLENLAQEWK
                     DVPMLARTHGQPASPTRLGKELMVFVYRLKEQLKFLKAVPYSAKFGGATGNFNAHYAA
                     YPAYCWKAFAVDFLENTLGLRREEFTTQISNYDNLAALFDALRRINTILLDLDRDMWL
                     YISMDYFKQKIKANEVGSSAMPHKVNPIDFENSEGNIGLANALFVHLADKLPVSRLQR
                     DLSDSTVLRNIGVPLAHTFIAIQSTMKGLDKLLLNPSVIQQDLDNHWAVVAEGIQTIL
                     RREAYPEPYEALKALTRTGQNITEASIQEFISKLNVSDAVKAELRNITPGTYTGN"
BASE COUNT         2919 a         2777 c         2670 g         2930 t
ORIGIN      
        1 ggctttcaac cagggccccc gcattgaaca atttgctccc gccagcggag acaaggccga
       61 ttttttcgaa acccgcgaca ttgaatcggt gatttcgctc aaagccgaaa cccacaactt
      121 ccccactaca gtagaaccct tcaacggagc ctccaccgga accggcggag aaatcagaga
      181 tcgtctgggc ggcggcaagg cttcattacc gatcgccggc acagcagttt acatgaccgc
      241 ctatacccgc aacgacaaaa accgcagttg ggaacaagcc ctacaggcaa gaccctggct
      301 ttaccaaacc ccggaacaga tcctgctcaa agcctcaaac ggagccagcg atttcggtaa
      361 caaatttggc cagcccctga tctgcggatc tttgctgacc ttcgaacacg aagagaatca
      421 aaaaaaatac gcctacgaca aggtcatcat gctggccgga ggagtgggct atgccaacaa
      481 aaaaaatgcg ctgaaagaaa ctccccatgc aggacaaaaa atagtagtgt tgggtggaga
      541 caattaccgc atcgggatgg gcggaggagc agtttcgtcg gtggacacag ggaaattctc
      601 caacgctata gaattgaacg cagtgcaaag agccaaccct gaaatgcaaa aaagggtggc
      661 caatgtaatc aggactttgg ccgaatccga tcagaaccct attgtctcca tacacgatca
      721 cggagcaggc gggcatctga actgcctttc ggaactggtg gaatccaccg gaggcctcat
      781 tgaaatggat aaattgccca taggcgaccc cactctctcg gccaaagaaa taatcggaaa
      841 cgaaagccag gaaagaatgg gactgctggt gaatgaagaa gatttggatc ttattcgccg
      901 gattgccgac cgcgaacgtg ccccgatgta cgtagtgggc gaaaccaccg gcgacatgcg
      961 tctggttttt aaagagaaaa acggagaccg ccccatagat ttgcaactcg accatatgtt
     1021 tggtaaagca ccgcgcacgg ttattaccga ccaaaccgtc gacgaatcgt tcagcgctct
     1081 aaattacgat ccggccaaac taaatgaata tattcaacgg gttttacagc tcgatgccgt
     1141 tgcctgcaaa gactggctca ccaataaagt agaccgctcg gtgaccggca aggtggcctg
     1201 tcagcaatgc gccggcgaaa tacagcttcc tgtttgtaat ttaggagctg ttgcgctcga
     1261 ttacagaggt aagtcgggta ttgccacttc gatcgggcat gctccccagg cagccataat
     1321 cgatccggaa gccggctctg tgcttgctat tgccgaagcg cttaccaaca ttgtttgggc
     1381 tcccctgagc ggcaatctct ccgctgtttc actttcggcc aactggatgt ggccctgcaa
     1441 gaatccgggc gaagatgcac gcctgtacaa ggcagtacaa gccgccagtg acttttccat
     1501 agctctgggt atcaatatcc ctaccggaaa ggattccctc tcgatgaccc aacagtacga
     1561 caaggagaag gtgttctctc cgggtacggt tattatttcg gcaggagccg aggtttcgga
     1621 tatccgcaaa atcgttactc ccgtaataaa accccaaaac gattacccgc tttactacat
     1681 tgatttctcg gaagatctgc ccaagctcgg aggctcagcc ctggctcaaa gcctgaacaa
     1741 actgggcaac gaagtgcccc gggtgaagaa cgcagcttat tttcgcgccg ctttcaacac
     1801 cgtgcaaagt atgctggaaa aaggtttgat tactgccggg cacgacattt cggccggagg
     1861 tcttataaca gctcttctcg aaatgtgctt ccccaatgca aacggaggac tcgacattga
     1921 tttaaattcc ctggccggcc tgcacgaaag caacaacgat ctgatccact tgctttttgc
     1981 cgaaaatcct tctgtgctta tccaggtaaa gaatcccgaa aaaaccgagg aatacctgac
     2041 acaagccggt atccgtttct gccgtctggg taactatatc cccgaacgca aactcaatat
     2101 acagttggaa gacaaaagaa tacaacagga catcgacgcc ttgcgcgata tctggtatcg
     2161 tccctcctac ttattcgatc gcaagcaaag cggagaaaaa gcagcgctgg aacgttattc
     2221 caattacaaa aaacagccgt tggaatttaa gttcgacaag aatttcaagg gtagcttcct
     2281 tcaatacggt atttctcccg accgcaaagg ccgcagcggc atcaaagccg ccatcatacg
     2341 cgaaaaagga gtcaacggcg accgcgaaat ggcttattgc ctccacctgg ccggttttga
     2401 tgtcaaggat gtccacatga ccgacctaat gtcgggccgg gaaacactcg aagatatcaa
     2461 catgctcgtc ttcgtgggag gcttttccaa ttccgatgtc ctggggccgg ctaagggttg
     2521 gtccggaggc tttttattca accccacagc caagaagagc ctcgatgcct tttacgcccg
     2581 tcccgacacc ctgagtctgg gtgtctgcaa cggctgccaa tgtatgattg ctctcgacct
     2641 gatttatccc gaacacaagg ataaacccag gatgatgcgc aacagttcgc aaaagtttga
     2701 atcgggattt gtatctgcga ttattcccga taatcattcc gttatgttcg gttctttgtc
     2761 cggcagtaaa ctgggcattt gggtagctca cggagaaggt aggtttgatt tgccttatac
     2821 cgaagataag taccatatcg tagccaaatt caattacgaa gcctatccgg gcaatccgaa
     2881 cggatcaaag ttttcaacag tcgccatttg ttcgaccaac ggcaggcacc tggccatgat
     2941 gcctcatttc gaaagagcta tcttcccctg gcaatgggct cactacccgg ccgacagaag
     3001 caaaacagac gaggtaacac catggataga ggcctttgtc aacgccagga aatggataga
     3061 aaagaaactg gtataaaggc aggcttaagc gagctgttct tcacttttct aaagctggga
     3121 gctttcacca taggcggagg ttacgctatg attccgctta tagaaaaaga attgatcgat
     3181 aaaaaaggct ggttcgaccg ggaagaattt ttggaactgc tggccctggc ccaaagtgct
     3241 ccgggaatta tcgcgatgaa tatcgccgtt tcggttggct acaaggccag aggaaaatcc
     3301 ggcatgtatt tggccagtct gggggcagtg ctgccttcct tcctgatcat cctgcttata
     3361 gcgtccgttt ttacttcttt cagggataat cctgtggttg aaagcatctt taaaggtgta
     3421 cggccggcag tagtcgcttt gattgctgca cccatctttc gattggcaaa atcagcccat
     3481 attaccctta aaaccttgtt tattcccatt ggcacggtta ttttagtggg ctttctggga
     3541 gtaaaccctg tctggattat ccttttgggc gccataggcg gtatagtctg gttttcgatc
     3601 gcaaaaagaa agcaactatg atctactggc aattattcat tacctactta aaaataggct
     3661 tcttcaattt tggaggagga tatgccatgc tttcgcttat cgagcacgaa atggtactga
     3721 agcaccagtg ggtcagtcac gctgaattta ccgacatggt ggccatttct caagtcactc
     3781 ccggccctat cggtatcaat atggccacct atgttggcta cacggtgacc ggcaatgttt
     3841 ggggatccat gctggccact tttgccgtaa gcctgccttc gctggttgtt atgtatgcta
     3901 ttgtacgttt tattcaggct ttcagacaaa acaaatggat ctcggcggca ttcgacggta
     3961 ttaaacctgt tacggttggg ctggtgggat ctgccgctat tttattgatg aacgaacaaa
     4021 acttctccga ctacatcagt ctgatcattt ttggttgcag ctttttgctc agctgggcgt
     4081 ttaaggtaca tcctattttg ctgattgttc tggccggatt gacagggctc cttgtttact
     4141 gatcaatata cccgcaacac aataccttcg ggagcacttt cactatgtac acggctcagg
     4201 gccgtcacca catacaacca atcgccggaa tagggatgtg agggtaaaac aaaatgatga
     4261 ttttgggtaa ttccgattat gtttctggga tcttcaatgt tttcgggttc ataacctttt
     4321 aagaatcgat agatgacaaa ataggccgga cgttctatct ctgaccttgg gcgtatggtt
     4381 ttccaggaca aatgttttcc gattttataa tcgtctctca agtccaattc atacacaggc
     4441 tcgggagcac ggtgttctgc cagaagatct accggaggta aggaaggata ataatgataa
     4501 ttcatcacca ggctgtccac aatgtatccg gtattgtttt ccagttcata ggccggccag
     4561 aaacaattcc ctttcaggcc gtcgttgttg cggacgaggg tcattttatc ctgtaactgg
     4621 ccgggattca tcgtccggac gatgtcctgt cccacataaa tggaactggc gaaattcaac
     4681 ttaccccacc attcggcaag ggtttgataa tcggctctgc tgtgtccgat ttcccaatac
     4741 aactgcggca cacaataatc aatccagccc tctttcatcc aaagcagggc atcggcgtag
     4801 aggtcgtcgt aattactcag gccgtcggtc tcactacctt gagggtcttg tttaatattg
     4861 cggtaaatac caaaagggct gatgccgaaa cgtacccagg gtttggtgtt gcggatggtt
     4921 tcgtgcaatt cccgcaccag ggtgttcacg ttgttgcggc gccagtcgtc tttttgcgat
     4981 gagttgtaac cctgcgactt accgtaattc ctaaaactgg catcgtcggg aaaatttaag
     5041 ccggctatgg gataaggata gaaataatcg tccatgtgga taccgtcgat gtcgtagttt
     5101 tgcactatct cagtcaccac tttgacgatg tgctcccggc agtcgggaat tcccggatcg
     5161 aaccaaaggg aactgccgta acggactatc caattacggt tcttgcggat aaaagtctct
     5221 ctgaatgcgc tttcgcgggc agtcaatttc acacggtagg ggttgatcca ggcatgaata
     5281 tccatgtttc ggcgatgaca ttcatctacc atgtaagcca ggggatccca gccgggattt
     5341 cggcccactc tgcctgttat gaattcactc caaggctcaa gcgaagaatt ataccaggca
     5401 tcggcctgag ggcgaatctg aaaaatgacc gtattgattc cggcctcatg caaaaggtcg
     5461 agcaaacggc tgaaatattg cttcattcca cgttcactca tagcagcata cttggtttgg
     5521 gcaaccgtat gcagccaggc tcctctgaac tcacgtttgg gaggcaagtt ctctgcctgt
     5581 ctccggctaa aaaaaacctc gggctcaggc agaggagttt gcggactttg acgattttgc
     5641 gttgcggcct gctgtagaat cagacataac gcgaaacata tcagaagtcg cctggccatg
     5701 gttttaattt tttgtttctc tcaaagccgc cacatcaaag cattttccct catctgccag
     5761 ctccgtgtta ggaaaaatgg cctgggcctg ctgcaataga atttcctcgt tggtataacg
     5821 ggcagaataa tgaccgatga tcaatttccc cactccggct ttctgagcta tccgggctgc
     5881 ctgtgaagct gtggagtgca aagtggcctc ggccagttct tccaaatcgt ctccaaacgt
     5941 agcttcatgg tacaaaaggt ttacaccctc gatgtatttt attatgtttt cgtcgtaggc
     6001 agtgtcggag cagaaggcgt aagacaaagc tgctttagga ggccgggtca gacgatcgtt
     6061 ggcgatcagc ctgccttcgg aatctacaaa gtcggctcct ccttttatgg caggaatctc
     6121 tttgagcggg atcccgtaaa aatcgatcat ctctttgatc atatgccgct ggcgaggttt
     6181 ttccctgaac aaaaaaccgg cagacttaac cctgtgtctc aaaggaaaac aatatacttc
     6241 cagactgcgg tcttcgtaaa ccagagagtg actgccggga tcgaacgaaa caaattccac
     6301 ctgaaagggc agatctctgc aaaaataatc caactgtggc tgcatcagtc cctcaagttc
     6361 cggcctggca tgtatgcgca gcggagcatt tctgcccaac aaacccatgg tcgaaattat
     6421 gcccaatagg ccaaaacagt gatctccgtg taaatgagaa ataaaaatat cgcctatacg
     6481 attgtaattc aaaccggttt gtctgaactg cagttgagtg ccttccccgc aatctatcat
     6541 aaacagcttc ccccgcagat gcacgatttg cgatgtggga taatgtcttt tggtaggcaa
     6601 agccgaaccg caacccagaa tatgcacatg aaaacaatcc ataggccggc cggtagatta
     6661 aaaatcggcg ttacgcggcg ttctcggaaa tgggattaca tcgcgaatat tcgccatgcc
     6721 ggttacaaaa agcaacaagc gctcaaagcc cagcccaaaa cccgagtgag gggctgtacc
     6781 gtagcgacgg gtatccaaat accagtatat gtctttgtcg ggaactccca tttctttggc
     6841 ccggctcagt agtttatcgt agttttcttc acgctgcgag ccgcctatga tttcgcctat
     6901 tttgggaaac aacacatcca tagcccgtac cgtcttgccg tcgtcgtttt gcttcatgta
     6961 gaacgacttg atttccttgg gataatcggt taagattacc ggttttttaa aatgctcttc
     7021 caccagataa cgttcgtgtt ccgatgccag gtcggcgccc cagtaaatag gaaattcaaa
     7081 cttatgtcct tgctcaacag ctgcttccag gatctcgatc ccttcggtat aggacaggcg
     7141 cacaaactgg ttatccacca caaatttaag gcggttaaac aagtcgtcgt cgtacatctt
     7201 gctcaaaaaa gccagatcgt cctgacaacg gtcaagagcc cattgtatgc agtattttat
     7261 gaaacgttcg gccaaatcca tattatcctg aatctcgttg aaagccactt cgggttctac
     7321 catccaaaat tctgccagat ggcggggcgt attggaattt tcggccctga atgtaggacc
     7381 gaaagtatag acagccccca gggccatcgc cgccaattca gcttcgagct gaccggaaac
     7441 agtgaggctg gtatgtttgc caaagaagtc ttcggaataa tcgatttcag ccttatcgtt
     7501 cttttttaaa ttattcagat tcagcgtagt cacctgaaac atttgccccg caccctcgca
     7561 gtcggaggaa gtaataatgg gtgaatggaa ataataaaaa ccgttgtcat aaaaaaaacg
     7621 atgtatggcc tgggccatct catgcctgat cctgaaaatg gctccaaagg tattggtcct
     7681 gggacgtaag tgagcaattt cccttaaaaa ctccagggta tgccctttct tttgcagagg
     7741 ataagtattg ggatcggcaa cgccgtacac ttcgatctct tgggcctgaa tctccgcttt
     7801 ttgaccctga gccggcgatt ccgaaagaat gccatccacc cggatacagg cgccggtggt
     7861 cagttgtttg agcagttctt catcaaaaga ggccagttct actacaatct gaatgttgtg
     7921 aatagtagaa ccgtcgttca atgcaataaa acccacctgt ttgtttccac gccgggtcct
     7981 gatccagcct ttgacctgca ctaaacttcc aaaatcttcg ctctgcagta aatcaacgat
     8041 ttttgttctt tgcaatttat tcatggctct aattcaactt taaacaaaag cacccatttt
     8101 cagcagggaa acttctctat cactaagata tctccagcgt ccgcgaggca gattcttttt
     8161 ggtcaagcct gcaaaataaa ccctgtccaa tttgtacacc ttgtagccga cctgttcaaa
     8221 gatacggcgc accaccctgt tctgcccgga atgtatctct atgccgatca cattcctttg
     8281 atccggagat acaaaactga tttcgtccac agctattttt tcaccgtcga gcactatgcc
     8341 ttcggctact tttttcatat cttcttcggt aaggtcccta tcgagagttg cctgatatat
     8401 tttacgtttt tcgtatttgg gatgagaaag cttggaagcc aattctccat cgttggtaag
     8461 caataaaact cctgttgtat tcctgtcgag ccttcctacg ggatatattc tttcgcggca
     8521 ggcatttttt accaaatcca ttactgtcaa gcgttcctcg ggatcatcgg aggtggttac
     8581 gcaatctttg ggcttgttga gcaaaacata gactttgtgc tcaatgctaa caggctgatc
     8641 atggaacaaa acttcgtcgg taggcttgat cttgaccccc agctcagtaa ccacttctcc
     8701 attcaccttg acgactcctg cctggataaa ttcatcggct tccctgcggg agcaaatacc
     8761 cgcgttagac aaaaatttat tcaagcgaat tggggtatcc ggatccagat caggagcgta
     8821 tttaggtata gacgtttctt taaaatagct gggaccatcg gaggaagatt ttttgatgcg
     8881 gggacgttta ggccggccat cggaacgcag tttttccgag ggttcgttga aattcggacg
     8941 gggacgatcc cggtaacctt cgttgctgaa gctacggcga ggacggtctg aaccgtaatg
     9001 ttcaaaacta ttgccacggt cgccataact gcggcgagga cgatcctgat aagcctccga
     9061 accgtaggaa cgacgcggac gatcctgaga accttcactg ccgaaagaac ggcggggacg
     9121 atcctgagag ccttcgctgc cgaagctgcg acggggacgg tccgaaccgt aatgctcaaa
     9181 actattcccc cggtcaacgt aactgcgacg aggacgatct tgataaccct cagaaccata
     9241 gggtcggcgg ggacgatcct gagaaccttc gctgtcaaaa ggacgacgag gacgatcctg
     9301 agaaccttcg ctgctaaaag gacggcgggg acggtcctga gagtcttcgc tactgaagtt
     9361 gcggcgggga cggtctgaac cgtaatgctc aaaactattg ccacggtcac cataactgcg
     9421 gcgaggacga tcctgataag cctccgaacc gtaggaacgg cggggacggt cctgggaact
     9481 ttcactgtcg aaagaacggc ggggacggtc gtaagagtct tcgccgtcga aagaacggcg
     9541 gtggtagtga tcgtgcgagc tttgatctga acttgttttc ttgttttgct cttcccggtt
     9601 gacagatgca ccttcgtcag gctttgctgt ccggttttca ttgtttgttg tcattgttgt
     9661 tttctaaaaa aataataagg aataacctct gcggttagga ctgtttgtat gtgttaattt
     9721 cctgtatatg tgccgggggt tatgtttctg agctctgcct ttacggcatc ggaaacattc
     9781 aattttgaaa taaattcctg aatagaggct tctgtaatgt tctgaccggt acgtgtcaaa
     9841 gcctttagcg cttcgtaagg ctccggatag gcttcacgcc taagtatggt ttgtatacct
     9901 tcggcaacca cagcccagtg attgtccaaa tcttgttgta tgaccgatgg attcagcagt
     9961 aatttatcca aacctttcat agtactctga atggcaataa aggtatgcgc caggggtaca
    10021 ccaatattcc tcaacaccgt ggaatcgctt aaatcccttt gcagacgtga caccggcaat
    10081 ttgtcggcca aatggacaaa caaagcattg gccaaaccta tgttgccctc ggagttttca
    10141 aaatctatcg ggttcacttt gtggggcata gccgatgagc cgacctcgtt tgctttgatt
    10201 ttctgcttaa agtaatccat agaaatgtac agccacatat ctctatcaag atcgagtaat
    10261 atcgtattga tgcgccttag ggcatcaaaa agggcggcta ggttgtcgta attggaaatt
    10321 tgcgtggtaa attcttcgcg cctaagaccc agcgtgttct ctaagaaatc aactgcaaag
    10381 gccttccagc agtaggcggg gtatgcggca taatgggcat tgaaattacc ggtagctcct
    10441 ccaaacttag cagaatacgg taccgctttc agaaacttta gttgttcttt gagcctgtaa
    10501 acaaagacca tcagctcttt acccaaacgg gtgggcgaag caggctgacc atgagttctg
    10561 gccaacatgg gaacatcttt ccattcttgc gccagatttt ccagccgtcc gcacaagcct
    10621 tcaatcaagg gatagtattg ctcctctact gcttctttta tggaaagagg cacggcggta
    10681 ttgttgatat cctgtgatgt caggccgaaa tggatgaatt ctttgtaagg atgtaggccc
    10741 agtttgtcga aaccctcctt gataaaatat tcaacagcct tgacgtcgtg gttggtaacc
    10801 tgttcaatct cttttatgcg ctgagcatct ccggcattaa agtctttgta tattttacgg
    10861 agaagctcaa atttctcatg cggaacttgt tttaattgtt ccaagggcaa acggcagagg
    10921 gcgataaaat attccacttc gacctgtatg cgatatcgca ttagggccaa ctcagaaaaa
    10981 tagtccgcca aacctgaagc tttgctgcga tacctgccat ctacgggaga tatggccgtc
    11041 aattcagtta aatccattcc cttttgttcg ttttataaaa gaagcggcgc aaagatacta
    11101 ttttgcaacg acatagccac atattccacc atttttcgtt ctttttttat aaaaagacca
    11161 tcagctgtct gaaaatttat caaataataa tttgccggga taaaaattca aattatcttt
    11221 gccgcgtttt ttaaaagcca agggcgttta gctcagctgg ttcagagcat ctgccttaca
    11281 agcagagggt cggcgg
//
LOCUS       DAOSGL010000035        11124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4942_length_11124_cov_4.418466, whole genome shotgun sequence.
ACCESSION   DAOSGL010000035 DAOSGL010000000
VERSION     DAOSGL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11124
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4942_length_11124_cov_4.418466"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /locus_tag="PKY68_02165"
     CDS             complement(<1..1896)
                     /locus_tag="PKY68_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOH23877.1"
                     /translation="MKNLLTSVCIIVLAGLFLSIPLFAAGPPAFPTAEGYGMYATGGR
                     GGKVVAVTNLLDDAQGSIPGSFRWALRQYPNEPLTIVFKTSGVINLVTQLRAKRTAGT
                     TIAGQTAPGDGICIRGAKCNFGGSQNLIIRHLRFRIGLKEFEGTDSTAFIEGGSIGIE
                     NASNWILDHCTFGWSGEENMTIYDNTYTTVQWCLVHEGLYNSGHGKGNRGYGAQWGGQ
                     TATYHHNLLAHNQSRSPRFNGARSNDIRVLIDYVNNVNYNWGGENSCYGGDMVEGRSH
                     YVNMINNYYKPGPARPGNRSSNFVRASFNGNQQTWQIPLWYMNGNYMEGSANSNCNTN
                     NYNGLNADEYIAKGIAKTSIISATPFDVPYAVITETAQQAYQSVLAGAGAFPRDTVDS
                     RIVDEARKGIAYYGGQTIGAGKGIIDRPSDVGGYPEYQTYDTIVDMDQDGMDDLWELE
                     HGLDPLNPEDRNLKLSSGYTVLEAYINGLVGEQIPFDTNDYRYYDIIVAKDGSGDFET
                     LSAAIDALPEGDERRLIYVKKGLYEEKVYIGSHSVSLNKVISIVGEHRDSVIISWNDY
                     NGKEIYYYGSSTPTIAGTPQSATMTVNASDFYMENVTVQNTYTSAQAVAIYHVGDRQT
                     FKNCRFKG"
     gene            complement(2111..3244)
                     /locus_tag="PKY68_02170"
     CDS             complement(2111..3244)
                     /locus_tag="PKY68_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23878.1"
                     /translation="MYDFNTKRFLFVWLLCSTLCVQALSPYAHRVFEYCPAPGQFINK
                     AYPAYEAGETYEDILKKVTKVLVGRKESGIVCLGSWGGYIVLGFDHAVQNKTDTVDFK
                     VYGNAHNNSAEPGIVCVSKDLNGNALPDDPWYELAGSGYYSDSTIKNYACTYYRPSPA
                     NGNVLWKDNLGDSGYVLRNDVYHSQASYYPLWVSEDSLVFSGSRLVSKASGGGTSWIS
                     PPYAWGYADNWPNNHSGSDFDIDWAVDEQGQPFYLDEIHFVRIHTAVQDQLGWLGEIS
                     TEISGLEDLHATMPDLGTDKPALRYLSTVRQLYVQAPPRSCLEVFNIQGVRVFIQEIE
                     GGILLNNGMEELVLVQILLDFLPQNMYVVRLSGNWGSVNSRIR"
     gene            complement(3286..6027)
                     /gene="feoB"
                     /locus_tag="PKY68_02175"
     CDS             complement(3286..6027)
                     /gene="feoB"
                     /locus_tag="PKY68_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOH23879.1"
                     /translation="MRLSELKTGEKAVIVKVYGHGGFRRRIVELGFVRGKVVECVMNA
                     PLNDPVDYKIMGYHISLRRSEAAMIEVVGREEVLQSDHQEKFRATIEEDDLRVVALKK
                     RKHIRVALVGNPNSGKTTLFNQVSGERKHVGNYGGVTVSASTGFMDFEDYSFSLVDLP
                     GTYSLSAYTPEEVYVRKHLDQEFPDVVINVVDAGNLERNLFLTTQLIDMNVRMVVALN
                     MYDELQQSGAKLDYMQLGRLLGVPFVPIVARNNQGLDRLFRIVIKVYEGADLFASEGL
                     DRDSLRELHDYHHRHDLSHDTLEELEEDEHYKTFGQGFRNCRRKRRHLHGRFHFRNHH
                     REHKHEHEYRIGEVVRHIHIYHGPVIEKSIEKIKAALALNEDLCNRYSMRFLSIKLLE
                     GDALVEELVRQLPNAAKIFELRSLSYKKIQQYLDEDAESAITNAKYAVIHGALKEVMT
                     PADKKQQLPKTHSLDAVFTHRYWGYPIFLLFLFLMFQATFVLGKYPMEWIESGVGWLG
                     SAIGKWMDPGPLRDLLIDGVIGGVGGVIVFLPNILILYFFISFMEDSGYMARAVFIMD
                     KLMHKIGLHGRSFIPLIMGFGCSVPAIMATRSIENRNSRMITILVIPLMSCSARLPVY
                     LLFAGVFFPNYAGLVLFGLYMLGILLAIVMAKVFKKAFFPEEEMPFAMELPPYRLPGF
                     KAILLHMWEKAHQYLKKMGTVILVASILIWFLGYFPRLDLPGNEQLVQMEQITAQEQT
                     EQAQIQVLNREGSQTAGAAEKAQWEGLSSLERQRMEQQEQSYIGRMGIWMEPVFRPLG
                     FDWKISVSLLTGVAAKEIIISTLGVLYAGDPNEEAAVLSERLQTASYADGSPVINAAV
                     ALSLMVFVLIYFPCVATLAAIRSETGSWKWAVFAVIYTILLAWCLAFVVYRLALLFG"
     gene            complement(6128..6298)
                     /locus_tag="PKY68_02180"
     CDS             complement(6128..6298)
                     /locus_tag="PKY68_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH23880.1"
                     /translation="MAYVINDDCIACGTCIDECPVDAISEGDIYKIDPELCTECGACA
                     DVCPTEAIHLDK"
     gene            6434..7147
                     /locus_tag="PKY68_02185"
     CDS             6434..7147
                     /locus_tag="PKY68_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOH23881.1"
                     /translation="MVKKVYPIVLLFLLLASPGQIIAGSPVRYLPYEDLKLYHFGFFI
                     GMHTQDLHIDHSGMPDNNGAVWYGSVPSYAPGFSVGVLGDYRIADFLSLRLSPAIHFG
                     TKDLALVSDQPGSEIITTSIRSNYIMVPLSIRYRGARSDNFRPYLMSGLSLGLDAGRR
                     KRQEVLLKAINYYWEFGFGCDLYLPYFRLVPEIKFCLGLGDVFEHKRSDQGSDAYEQF
                     TNAFDRMTSRLIVLSFQFE"
     gene            complement(7150..9417)
                     /locus_tag="PKY68_02190"
     CDS             complement(7150..9417)
                     /locus_tag="PKY68_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH23882.1"
                     /translation="MNPEPVRSLHAQEEIIKSNAGLGLAYDYVCHTGESVFLTGKAGT
                     GKTTFLQRIKKEVNKRTVVLAPTGVAAINAGGMTIHSFFQLPFGPYVPGPKPEKSGEG
                     RRQIHKLRKDKINLIRSLDLLIIDEISMVRADLLDAVSDNLCRYRRDSRPFGGLQLLM
                     IGDVQQLAPVVKDDEWALLRTVYDSPYFFSSQALSKMPYTTIALTEVFRQSDAFFLDL
                     LNKVRENKLDEAGLKALNSRYISGFNPDDNEGYITLTTHNYQAQSINEAKLAAIAQPE
                     FCYKARLQGLFPESSFPTNQELLLKKGAQVMFVKNDSSPERLYFNGKIGRVTDLCEDY
                     ICVQCEGDAREVVVQREQWFNTKYTLDPETKDIEETVEGVFEQYPLKLAWAITIHKSQ
                     GLTFEKAIIDAASAFAHGQVYVALSRCKTLEGIVLNRPLNPWALKDDAVVSAFSREIL
                     NHPADPQHLLQAKRRYFVDRLLDLFDFQELLLALRLFNRQCRDHLEALYPSYAADVGK
                     ACSACVNEITGVGLAFGNQISRISEACAEPEQEPQLQERVQKAGLYFSEKLQGIIGTV
                     LAENLPEMDNKESRQSLENAFNRLEQEFDYKAGLMREFSLRAFSVGDYLNLRAKLLLK
                     SEEPKKKSGSKKEKKPKKESAEVHYYGDNNEMDASRNSYSDEAYTESVVTVGSDITHP
                     ILYGQLTAWRRQEARELGRPAYTVLSQAALLGICRQPPVNTRELKKIRGIGPKTLEKY
                     GERLMEIIDSYLGML"
     gene            complement(9414..10130)
                     /locus_tag="PKY68_02195"
     CDS             complement(9414..10130)
                     /locus_tag="PKY68_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOH23883.1"
                     /translation="MQNNSLNTAAGVKATLLSYARSEKAAVLSRFFKTGPGQYGEGDL
                     FCGVTVPQVRSVARQVLDIGRDEIEILIQDDLHEVRQCGFFILVEQYRKQPPKRAEIV
                     DYYLSRLYRANNWDLVDLSAPKILGHWLLNHDRSVLYRLSESTVLWEQRAAVVSTMTL
                     IHRNQFDDTLALVKKQMQHPHDLMQKANGWMLREIGKRDRSVLTAFLEEYAARLPRTT
                     LRYAIEHYSAEERQYYLKKK"
     gene            complement(10112..>11124)
                     /locus_tag="PKY68_02200"
     CDS             complement(10112..>11124)
                     /locus_tag="PKY68_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOH23884.1"
                     /translation="TIRHQKVDYPASWLGIPDKAALPALYRHFGVDNMVALKAKINDD
                     IFPVEMPYHSPTSNAIYAAFDFAKKKHGLSDERTLTAPGFFEDYSDPADVDKFDWPDP
                     EKYIDPALCRKVVDEVPEGYAVMGVVWSAHFQDACAAFGMETALMNMMLAPELFMAVT
                     NRIVDFYLKANEIFYQATRGKLHAVLIGNDLGSQNGLMISPEAVREFVLPGTRKLVQQ
                     AKRFGLEVMHHSCGSIHDVIPDLMEAGADIIHPIQALAAHMEPDRLKKDFPSASFCGG
                     VDAQYLLVHGSPDDVRVKVRELKALFPTGLIFSPSHEAILQDIPPANIEALFDEVCKT
                     TP"
BASE COUNT         2817 a         2889 c         2573 g         2845 t
ORIGIN      
        1 acctttaaac cggcaatttt tgaacgtctg gcgatcgccc acatggtaaa tggctaccgc
       61 ctgggcagaa gtataggtgt tttgcaccgt tacgttctcc atgtagaaat cggaagcatt
      121 gacggtcatg gtcgccgact gcggtgttcc ggctatcgtc ggtgtgctgc tgccgtaata
      181 ataaatttct ttgccgttgt aatcgttcca gcttatgatt acgctgtcgc gatgctcgcc
      241 cacgatactg attactttgt tcagggaaac ggaatgcgaa ccgatgtaca ctttttcctc
      301 gtaaaggcct ttttttacgt aaatcaaacg gcgttcatcc ccttcgggca aggcatcaat
      361 ggcggcagac agagtctcga agtcaccgga accatctttg gccacaataa tatcgtaata
      421 acggtaatcg ttggtatcga aaggaatctg ttcgcccacc agaccattta tatatgcttc
      481 caatacagta taaccactgc ttaatttaag attgcgatcc tcgggattga gcggatccaa
      541 tccgtgttcc agttcccaga gatcgtccat gccgtcctga tccatgtcga cgatggtatc
      601 gtaggtttgg tattccgggt aaccgcccac gtccgaaggc cggtcgatga tgcctttgcc
      661 ggctcctatg gtttggcctc cgtaataagc gattcctttg cgtgcctcat ccacaattcg
      721 gctgtctacc gtatcgcgag ggaaggctcc ggccccggcc agcacacttt ggtaagcctg
      781 ctgggctgtt tccgtaatca cggcataggg tacgtcgaag ggggtagccg aaataatgga
      841 ggttttggca atccctttgg ctatatattc atcggcattc aggccgttgt agttgttcgt
      901 attgcagttg ctgttggccg aaccttccat ataattgccg ttcatatacc acaggggtat
      961 ttgccaggtt tgctgattgc cgttaaagga ggcccgcaca aaattggaag atctattacc
     1021 gggacgagca gggcccggtt tatagtaatt attgatcata ttcacataat gggagcgacc
     1081 ctccaccata tctccgccat aacacgaatt ttcgcctccc cagttgtagt tgacgttgtt
     1141 cacataatcg atcagcaccc ggatatcgtt gctgcgggca ccgttgaaac gcggacttcg
     1201 actctggttg tgggccaaca gattatggtg gtaggtggcg gtttgtccgc cccattgggc
     1261 gccgtatccg cgattgccct tgccatgtcc tgagttgtac aaaccctcgt gtaccaggca
     1321 ccattgaact gttgtatagg tgttgtcgta aatggtcata ttttcttcgc ccgaccaacc
     1381 gaatgtgcaa tgatccagga tccagttgct ggcgttttct atacctatgg agcccccttc
     1441 gataaaggcg gtgctgtcgg tgccttcgaa ttctttaagg ccgatacgga atcgcaaatg
     1501 cctgatgatc aggttttgag agcccccaaa attacatttg gccccgcgta tacagatgcc
     1561 gtcgcccgga gccgtttgtc cggcaatggt ggtgccggct gttcgtttgg ccctgagctg
     1621 ggtgaccagg ttaatcactc cggaagtttt gaaaacaatg gtcaggggct cattgggata
     1681 ttgtctgagc gcccagcgaa aactacccgg aatgctgccc tgagcgtcgt ccagcaaatt
     1741 ggtgacggcc accaccttgc ctcccctgcc cccggtggca tacatgccat agccttcggc
     1801 cgtgggaaaa gcaggaggcc ctgctgcaaa caagggaatg ctcagaaata aaccggctaa
     1861 tacaataata cagacagaag tcagaaggtt tttcataata cttactataa gaacggcctg
     1921 ttttctctcc atactactct caaagcaggg agcagtattt taaaaagaac cggcattggt
     1981 aatgatataa ataatccaca aaggaaataa aaaaacaact attcatccca actattgagg
     2041 aattttaaca agccccctga gcatacagaa cgtatctgaa gccggccgtg ttgccggctt
     2101 tgctctaatt tcaacgaatc ctcgaattta cggaacccca attgccggat aaacgcacaa
     2161 catacatatt ttgaggcaga aaatccaaaa gaatttgcac taagacaagt tcttccattc
     2221 cattgttcaa cagaattccc ccctcgattt cctgtataaa caccctgaca ccctgaatat
     2281 taaacacctc gagacagctt cgaggaggag cctgcacata cagttgccgg acggtactca
     2341 ggtagcgcag tgcgggtttg tcggtaccca gatcgggcat cgtggcgtgc aaatcctcca
     2401 aaccggaaat ttcggtagaa atttcgccca accaacccaa ttgatcttgt acggcagtat
     2461 ggatgcggac aaaatgtatt tcatccagat aaaacggctg cccctgctcg tccactgccc
     2521 aatcgatatc aaaatctgac ccggagtggt tgttgggcca attatccgcg taaccccagg
     2581 cgtaaggagg agaaatccag gaagtgcccc ctccgctcgc ctttgaaacc agccggctgc
     2641 cgctgaaaac caaactgtct tcgcttaccc ataaaggata ataggaggcc tgcgaatgat
     2701 aaacatcatt cctgagcaca taaccactat ctcccaaatt atctttccac aaaacgttcc
     2761 catttgccgg cgaaggccgg taataagtac aggcgtaatt tttaatggtg gaatcggaat
     2821 aataccccga accggccaat tcataccagg ggtcgtcggg taaagcgttt ccgtttaaat
     2881 ctttgctgac gcacactata ccgggttcgg cgctgttgtt gtgcgcgttg ccatacactt
     2941 taaaatcgac ggtatcggtt ttattctgga cggcatgatc aaaacccagc acaatataac
     3001 cgccccaact gcccaaacaa acgataccgc tttctttgcg tccaaccagc accttagtta
     3061 cctttttcaa aatatcctcg tatgtttctc cggcttcata agcaggataa gccttattta
     3121 taaattgacc gggagccgga caatactcaa aaacccggtg agcataaggc gaaagagcct
     3181 gtacgcacaa agtactgcac aacagccaga caaacagaaa tcgttttgta ttaaaatcgt
     3241 acatctaaat acaacattcg gcatcaatgc caagcatggc caaaatcagc caaacaacaa
     3301 agctagacgg tacacgacaa aggccagaca ccaggcaagc aaaatggtat aaatcacggc
     3361 aaaaacagcc catttccagc tgccggtttc ggatcgtata gccgccaacg tggccacaca
     3421 ggggaaataa ataagcacaa agaccatcaa acttaaagct acggcggcat tgattaccgg
     3481 gctgccgtcg gcataacttg ccgtctgcag gcgctcggac aaaacagcgg cttcctcgtt
     3541 gggatcgccc gcatagagca cacccaaagt gctgatgata atttctttgg cagcgacacc
     3601 tgtcaacagc gaaacactta ttttccagtc gaatcccagg ggacgaaaca ccggctccat
     3661 ccatattccc atacgaccta tataggattg ttcctgctgt tccattcttt gccgttccaa
     3721 gctgctcaat ccttcccatt gcgctttttc ggcggctccc gcggtctgac tgccctcccg
     3781 gttcagcact tgtatctgcg cctgctccgt ttgctcctgc gccgttattt gctccatctg
     3841 tacaagttgc tcattaccgg gcaaatcaag acgaggaaaa tagcccagga accaaatcaa
     3901 aatggaagct accagtataa ctgtccccat ttttttaaga tactgatgtg ctttttccca
     3961 catatgaagc aagatagcct tgaaaccggg taggcgataa ggcggcaatt ccatggcaaa
     4021 aggcatctct tcttcgggaa agaaagcttt tttaaacact ttggccatca ctatagccag
     4081 caaaattccc aacatatata agccaaaaag cacgaggccg gcgtagttgg ggaaaaaaac
     4141 tccggcaaaa agtaaatata ccggcaaacg tgcactgcaa gacatcaggg gaatcaccag
     4201 aatggtgatc attctgctgt tgcggttttc tatggatcgg gtggccataa tggccggcac
     4261 actgcaaccg aaacccataa tcaagggaat aaaggaccgg ccgtgcagac ctattttatg
     4321 catcaattta tccattataa agacggccct ggccatatag ccggaatctt ccataaagga
     4381 gataaaaaag tacaagatca gaatattggg caaaaacacg atgacacctc ccacgccgcc
     4441 aatcactcca tcaataagta aatccctcaa tggcccggga tccatccact tgcctatggc
     4501 cgagcccagc caccccactc ccgattctat ccattccata ggatatttgc ccaagacaaa
     4561 ggtagcctga aacatcagga acaaaaagag caaaaaaata ggataacccc aataacgatg
     4621 agtaaatacc gcatccaggg aatgagtctt gggtaattgt tgcttcttat cggcgggggt
     4681 cattacttct ttgagagctc cgtgtattac ggcgtatttt gcgttagtaa ttgccgattc
     4741 ggcatcttca tcgaggtatt gctgtatttt tttgtaagac aaggatctca gctcaaatat
     4801 cttagcggca ttgggcaact gcctgaccag ttcttccacc agggcatctc cttccaggag
     4861 cttaatggac aaaaagcgca ttgaataacg attgcacaag tcttcgttca aagccagcgc
     4921 cgccttgatc ttttcaatac ttttttcgat aacgggaccg tggtatatat gaatatggcg
     4981 taccacttcg cctatgcggt actcgtgttc gtgcttgtgt tccctgtggt gattcctgaa
     5041 atgaaaccgg ccgtgcaaat gacggcgttt tcttctgcag ttcctaaagc cttgcccgaa
     5101 agttttgtaa tgctcatcct cttccaattc ttcgagcgta tcgtgcgaca agtcgtgccg
     5161 gtggtgataa tcgtgcagct ctctgagact atcgcgatcc aaaccctcgg aagcaaaaag
     5221 atccgcgcct tcgtatacct tgattacgat ccgaaacaaa cgatccaaac cctgattgtt
     5281 acgagctacg ataggcacga acggaactcc cagcaagcgg cccagttgca tgtaatccag
     5341 cttcgctccg ctctgctgca attcatcgta catattcagc gctaccacca tacgcacatt
     5401 catgtcaatg agctgtgtgg tcagaaaaag attgcgctcc agattgcctg catccaccac
     5461 attgatcact acatcgggaa actcctgatc gaggtgtttg cgcacataca cctcttcggg
     5521 ggtgtaagcc gacaatgaat aggtgccggg caaatcgacc aaactgaaac tataatcctc
     5581 gaaatccata aatcccgtag aggccgaaac cgtcactcca ccgtagtttc ccacatgttt
     5641 acgttcgccc gaaacttgat tgaataatgt agttttaccg gaattgggat tccccaccaa
     5701 ggctacacga atgtgcttac gttttttcaa ggctacgact ctcaggtcgt cttcttcgat
     5761 ggtagcacga aacttttcct gatgatccga ctgcaacact tcttcccggc ctacgacttc
     5821 gatcatggcg gcttcgcttc gacgcaaaga aatatgataa cccataatct tgtagtcgac
     5881 ggggtcgttc agcggagcat tcataacaca ctccactacc ttacccctga caaagcccag
     5941 ctcaactatg cgccggcgaa aaccaccgtg cccatagacc ttgacaataa ccgctttttc
     6001 tccggttttc agttcggaca gtctcatatc accctcattc tgacgaaaaa aagccgccta
     6061 aaaatctata gacagcttcc ttttcaccgc aaagtcgttt cgatgcctcg accaggcagg
     6121 aatcgcttta tttgtcgaga tggatggcct cggtcggaca aacgtccgcg caagccccac
     6181 actctgtaca caactccgga tctattttat agatatcacc ttcggagata gcgtcaacag
     6241 gacattcgtc tatgcaagtc ccgcatgcga tgcaatcgtc attaattacg taagccatag
     6301 tttgattaag tttttaaaca aattcgaggc aaaagtaatt cttttttgag cagtatacca
     6361 aactatttgg caataattga cgagatttat cgttttatcc cccgaatgtc ctttgtttga
     6421 acccgtgctc aagatggtaa aaaaagttta tccaatcgta ctgcttttcc tactgctggc
     6481 ttctcccggg cagattatcg ccgggagtcc ggtccgctac ctcccttacg aagatcttaa
     6541 actctaccat tttggatttt ttatcggcat gcacactcag gacctgcaca tagatcattc
     6601 aggcatgccc gacaacaacg gagccgtctg gtacggcagc gttccttcct atgctccggg
     6661 gttcagtgtg ggcgtgttgg gagattaccg catagccgat ttcctgagtt tgcggctaag
     6721 ccccgccata catttcggaa ccaaagacct ggctttggta tcggaccaac ccggctccga
     6781 aattattacc acctccattc gttccaacta cataatggtt cctctgagca ttcgttaccg
     6841 gggagctcgc agcgataatt tccgacctta cctgatgagc gggctcagcc tggggctgga
     6901 tgccggccgg cgaaaacgcc aggaggtgtt gctcaaagcc ataaactatt actgggaatt
     6961 cggtttcggt tgcgacctgt atctgcccta ttttcggctg gttcccgaaa tcaagttttg
     7021 cctgggactg ggcgatgtgt ttgagcataa acgctccgat cagggcagcg atgcttacga
     7081 acaattcacc aatgccttcg accggatgac ttcccggctg atagtattat cgttccagtt
     7141 tgaatagcct cacaacatac ccaggtagct gtcgataatc tccatcagcc tttcaccgta
     7201 tttttccaag gttttgggcc ctatgccccg aatttttttc aattccctgg tattcaccgg
     7261 aggctggcgg caaatgccca acaaggctgc ctgtgagagt acggtgtatg ccggacgccc
     7321 cagttcgcgg gcttcttgtc gcctccaggc tgtcagttgc ccgtaaagga taggatgagt
     7381 tatatcgctg ccgacagtaa cgacggactc tgtataagct tcgtcggagt agctgttccg
     7441 agaggcatcc atttcattgt tgtcaccgta gtaatggacc tcagcactct cttttttggg
     7501 ttttttctcc tttttactcc cactcttctt tttgggttct tcggatttga gcaaaagttt
     7561 ggctctgagg ttaagataat ctcctaccga aaaagctctc agcgaaaact ccctcatcag
     7621 acccgctttg taatcgaatt cttgttccag gcggttaaag gcgttttcga gcgattgcct
     7681 gctctcttta ttgtccatct caggcaagtt ttccgctaac acagttccga ttatgccctg
     7741 caatttttcg gagaaataaa gaccggcttt ttggacacgc tcctgtaatt gtggctcttg
     7801 ttccggctcg gcacatgctt cggatattcg actgatttgg tttccaaaag caaggccaac
     7861 gccggttatt tcgttaacac aggcagaaca agccttgcct acatctgctg cgtaggacgg
     7921 gtataaagct tccaaatgat cgcgacattg ccggttaaat aaacgcaggg ccagcaaaag
     7981 ctcctgaaaa tcgaataaat cgagcaaacg atcaacgaaa tacctccgct tggcttggag
     8041 caagtgttgc gggtcggccg gatggttcag aatctctctg ctgaaagcgc tcaccacggc
     8101 atcgtccttg agcgcccaag ggttcagggg gcgattgagc actattccct cgagggtttt
     8161 gcatcggctc aaggccacat agacctggcc atgggcaaat gcagaagcgg catctataat
     8221 tgccttttca aaagtcagac cctggctttt gtgtatggtg atggcccagg ccagtttgag
     8281 cgggtattgt tcaaaaacgc cttcgactgt ttcttcgata tctttagttt cgggatccag
     8341 ggtgtatttg gtgttgaacc attgctcacg ctgaaccacg acttcccggg catcgccttc
     8401 gcattgcaca catatataat cctcgcacag atctgtaacc cggcctattt tcccgttgaa
     8461 atacaacctt tcgggagagg aatcgttttt gacaaacatc acctgagctc cctttttgag
     8521 cagcaattcc tgattggtgg gaaaagatga ttcggggaac aaaccctgca acctggcctt
     8581 gtagcaaaat tcgggctggg caatggctgc caatttggcc tcgttaatcg attgggcctg
     8641 ataattgtgc gtggtcaggg tgatgtaacc ctcgttatcg tcgggattga aaccacttat
     8701 atacctactg ttgagagctt tcaaaccggc ttcgtcgagc ttgttttcgc gcactttgtt
     8761 gagcagatcc agaaaaaagg catccgattg cctgaatact tcggtaaggg ctatggtggt
     8821 gtaaggcatt ttcgacaaag cctgactact gaaaaaatag ggagaatcgt acaccgtacg
     8881 taagagagcc cattcgtcgt ctttgaccac cggggccaac tgctgcacat cgcctatcat
     8941 caacagttgc aagccgccaa aaggccggct gtctctgcgg taacggcaaa ggttgtcgct
     9001 gacggcatcc agcaggtcgg cacgtaccat acttatttcg tcgataatca aaaggtcgag
     9061 gctgcggata agattgattt tatccttgcg caacttatga atttgtcgtc tgccctcgcc
     9121 cgatttttcc ggcttaggcc cgggaacgta gggaccaaaa ggcaattgaa aaaaggaatg
     9181 aatggtcata ccgcccgcat tgatggcggc tactcccgta ggagccaaca ccactgtgcg
     9241 tttattgact tcttttttga tccgttgcaa aaaagtggtc tttccggtac cggccttacc
     9301 ggtcaaaaaa acagattcgc cggtatggca aacataatca taagccaacc ccaagccggc
     9361 attgctcttg attatctcct cttgtgcatg caacgatctg actggctccg gattcatttc
     9421 ttctttaaat aatattgcct ctcttcggct gaataatgtt caatagcgta acgtaaggtg
     9481 gtgcggggca gccgggcagc gtattcttcc agaaaggcag tcaacacact gcggtcgcgc
     9541 ttgccgattt cacgaagcat ccagccgttg gccttctgca tcaggtcgtg cggatgttgc
     9601 atttgctttt tgaccagagc cagggtatcg tcaaactgat tacgatggat cagggtcatg
     9661 gtgctgacta cggcagctct ctgctcccaa agcaccgtgc tctcgctgag acggtacaac
     9721 acggagcgat cgtgattgag caaccaatga ccgagtatct tcggagcgct caggtccacc
     9781 aggtcccaat tattagcccg gtataaacgg cttaaataat aatcaactat ttcagctcgc
     9841 tttgggggct gtttgcgata ttgttccacc agaataaaga atccgcactg gcgtacttcg
     9901 tgcaggtcgt cctgaatcag gatttcaatt tcgtctctgc ctatatccaa aacctgccgg
     9961 gccacagaac gtacctgagg caccgtcacc ccgcaaaaaa gatcaccctc gccgtattgg
    10021 ccggggccgg ttttgaaaaa gcggctcaac acggcggctt tttcgcttcg ggcataactc
    10081 aaaagggttg cttttacccc ggcagcagta ttcagggagt tgttttgcat acttcgtcga
    10141 atagagcttc aatgttggcc gggggaatgt cttgtagtat ggcttcgtga ctgggagaga
    10201 aaatcagacc ggtcgggaaa agagctttga gttctcttac cttgactcgc acatcgtcgg
    10261 gactgccgtg taccaacaag tactgagcat caacccctcc gcaaaagctg gcagagggaa
    10321 aatccttttt gagcctgtcc ggttccatat gagcagccag agcctgtatg ggatgaatga
    10381 tatcggcccc tgcttccatc aggtcgggaa tcacatcgtg gatggaaccg caggaatgat
    10441 gcatcacttc caacccgaaa cgtttggctt gctgtaccag ctttcgggta ccgggcaaaa
    10501 caaactccct gacggcttcg ggcgaaatca tcaggccgtt ctgactgccc aggtcgttgc
    10561 caatcaaaac ggcatgcaat ttgcccctgg tagcttggta aaaaatttcg ttggccttaa
    10621 ggtagaaatc cacaattctg ttcgtaacgg ccataaacaa ttccggagcc agcatcatgt
    10681 tcatcagggc cgtttccatg ccaaaagcgg cacaggcatc ctgaaaatgg gccgaccaaa
    10741 ccacgcccat aacggcataa ccctcgggca cttcgtccac tactttgcgg caaagcgccg
    10801 gatcgatgta tttttcgggg tcgggccaat cgaatttatc cacatcggcc ggatcgctgt
    10861 aatcttcgaa aaagccggga gccgtcaggg tgcgttcgtc ggacaggccg tgctttttct
    10921 tggcaaaatc gaaagcagca tagatggcgt tactggtggg cgaatggtaa ggcatctcta
    10981 caggaaaaat atcgtcgttt attttagcct tgagagccac catattgtcc acaccaaaat
    11041 ggcggtacag ggcaggtaat gccgctttgt cgggtatgcc caaccaggaa gcaggataat
    11101 ctaccttttg atgtcttata gttg
//
LOCUS       DAOSGL010000036        11086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_4963_length_11086_cov_4.268063, whole genome shotgun sequence.
ACCESSION   DAOSGL010000036 DAOSGL010000000
VERSION     DAOSGL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4963_length_11086_cov_4.268063"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1664)
                     /gene="rmuC"
                     /locus_tag="PKY68_02205"
     CDS             complement(162..1664)
                     /gene="rmuC"
                     /locus_tag="PKY68_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOH23885.1"
                     /translation="MNFLYLLAAIVASGIIASLISVLSIKARTVPKAQLEELNKLAQE
                     SKTALLIEQEKNNSLAAQLKTAEEQTAALNTTINDLTKSEAEYSARLSSAQESLKEKS
                     LSFEQLKEELRLKTEEFNNANKILAEFRANNLALNEKLETQKKEIEELAKKFNTEFEN
                     IANKVLEEKTQKFTAQNQSNLEQILKPLGENIDQFKKKVEEVYNTEAKERFSLGKEVQ
                     RLVELNQQVSKEANNLTNALKGSSKAQGDWGQMILENILEKSGLVKDREYFVQEFLKD
                     EDGNYLKNEQGNKMQPDVIVAYPDDRKIIIDSKVSLTAYTRYVAADDPEEQQKALKEH
                     LNSMKKHIDELSVKSYQDYASSLDFVMMFVPNEPAFMVAMQQDAEIWNYAYQKRIILI
                     SPTNLIAALRLIVDLWKREYQNQNAQAIAERGKLLYEKLVGFVDDLSSLGGHLDKARS
                     SYSDAFDKLKSGRGNLISQADKLIKLGVKAKKNLPGSLVSDALMEEGETE"
     gene            complement(1687..4737)
                     /locus_tag="PKY68_02210"
     CDS             complement(1687..4737)
                     /locus_tag="PKY68_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOH23886.1"
                     /translation="MSFIKKFKKKGVIGLYRRFVSEGIGRFRHTVIGSLLIIMFTAGF
                     HLQAQTINLDSLLTKEELNWLQLNKDKIRYTPDPSWPPANYVEDGVHKGFVSDYICLF
                     EQMLGVEFERVYYPTWTGMLDGLKKGETDFVGGIQRSALREEYLRFTKPFFRVELGLI
                     TRANYPTEVSKRQINSMKVACVEGYTSTYYIEEAYPSAQIVYVNNEYEALLQVVYGIT
                     DGAVVDYMTANYLVQNKGLTNLKHAVFLDYKWNLRFAARRELPELCSILGKLLDQVDE
                     EQHQAFVSKWSNNDILGGYKHGFYESNKKIITGIFFLALFALVVVGIFNLLLRKRVNK
                     QTTALKEAWDKVKKSEEKYKLLAENTTDLIWLSDLKLNLSYITPSVEKMLGFTPEEFL
                     ALPLDKMIPIDQVERVMDMIKEEFRLEQQDPEIEKNRSRSVEVEHFHKDGRVIWVSIN
                     ATFVRDKSGKPIGLHGVTRDINEKKKIGDYIEKRLAVETLLSRTSRLAIGYYAQDTAF
                     NTILKDIGRTLNFCRTYIFEYDDKNQILSNTYEWCAEGFQNYKNIFQRIQYKDVAWLI
                     DRLKQGKIVQYRNIEDIPDEATKKFFRFLRILSLLNVPLMIEDRFYGFIGFNECKTYR
                     DWQPENIKALESLAYIVTSMLERHKTEKEMIKKDRSIELSMIGKAIIDMEGKIIYANK
                     ACLNYWGYNALEDVLNKEVFGYWASETELRELLKVVMEKGEWQGESKAKRADGSFFDV
                     LLSSSMVTDKNGEAICIQASIIDNTERKAREKELIKAKEKAEESNRLKTAFLSNISHE
                     IRTPMSGILGFVQLLQTSDLSDKEKDEYFKSVNLSGKRLIETLNDIIEISRIESGELE
                     MQENETDISEIVQFIRETYKPQAVEKNLQFRINTPSESSNLRFITDVYRLRRILSNLV
                     KNAIKYTDEGSVELGASIEEDQVCFFVKDTGRGISADRTEAIFDRFVQADLNLTREHE
                     GAGLGLSISKAYVEQLGGKILIKSKEKEGSTFSFSIPYKAPAKT"
     gene            5042..5401
                     /locus_tag="PKY68_02215"
     CDS             5042..5401
                     /locus_tag="PKY68_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23887.1"
                     /translation="MEKTTFLVTYLVSFLVCAGVTLGLIALINKSLKVYFKQLCQNAQ
                     VADLFIRMTKIVILLAGLGAALTSNYTTNEKADWLSLTWNVTAQVKAALESLFIAFII
                     LALAFLVLHVCTRICKK"
     gene            5398..5787
                     /locus_tag="PKY68_02220"
     CDS             5398..5787
                     /locus_tag="PKY68_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23888.1"
                     /translation="MTYWTSTQIALIYFGGIALAILSAVLVLLYLKHCRRAFLVIWTK
                     FRKTSIWGFKSSLIVAALLGALSVNYLDCSGEYEYLKESGYLVLTKGLEQIGTALVLL
                     SITTGAWLLVFIIRMLVNRRSKSEKQS"
     gene            complement(5809..6927)
                     /locus_tag="PKY68_02225"
     CDS             complement(5809..6927)
                     /locus_tag="PKY68_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOH23889.1"
                     /translation="MEIKKISTKKELREFVSFPIKLYKDHPYFVPPLIFDELNTFNPA
                     KNPALEFCEVACFVAKEGKTLLGRIAVILNKEANKRFDEKHVRFGWFDVVDNPEVTEA
                     LMGAAADWARQRGMEAIKGPMGFCDFDNEGMLIHGFDQLGTFATLYNYPYYPQHLEQL
                     GFRKEVDWVEFQIDVPEELSERYFRFAELLAQKFDLKVVSCKSTRELAKRYGKKIFQL
                     LNDSYDVLYGYVPLNEKQVDYYIKLYLSFVRPELVSLVVTPEDRVVGLGIAIPSMSKA
                     FQKAKGKLLPFGFIHMLKAIRKNDLVDFYLMGIHPEYQSKGLNSLIFTDMLPKFQKNG
                     YRLAETNPELETNSRIQALWSEFGPRHHKTRRIYIKEI"
     gene            complement(6977..8563)
                     /locus_tag="PKY68_02230"
     CDS             complement(6977..8563)
                     /locus_tag="PKY68_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOH23890.1"
                     /translation="MDIKTEIKRRRTFAIISHPDAGKTTLTEKLLLMGGAIQVAGAVK
                     SNKIRKTATSDFLEIERQRGISVATSVMTLDYKGKRVNILDTPGHKDFAEDTYRTLTA
                     VDSVVLVIDSVKGVEEQTRRLMEVCRMRAIPVMVFINKMDREGKDPFDLLSELEEELK
                     IATCPFTWPIGEGSRFKGVYHLYRKDVRLYDPQKTLKSTELLNTKDLNNTELRRIVGQ
                     DLINKLKEDMELIPGLFESFNLSLYREAYLAPVFFGSALNSFGVPELFDSFVEIAPGP
                     AALKTAERLVQPEEEKFSGFVFKIHANLDPNHRNRMAFLRISSGRFERNKIYYHCRLD
                     KNMRFSAPATFMANNKSTVDEAYPGDVIGLYDNGNLKIGDALTEGEILTFKGIPNFAP
                     EILKEVINADPMKAKQFEKGLRQLTDEGLAQLFVQEQGKRKIIGTVGELQFDVIQFRL
                     EKEYGAKCRFQLLPYAKACWFGSDNRAQLEEFIKRKAAHIVFDKNNRAVFMAESDWML
                     NNSRETFPDIRFTMSSEFNI"
     gene            complement(8586..>11086)
                     /locus_tag="PKY68_02235"
     CDS             complement(8586..>11086)
                     /locus_tag="PKY68_02235"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH23891.1"
                     /translation="FQLSLMKAANFILTAFLMLLPPAAKAIQDSSLQVHYTFLNEGFT
                     VSDQSGNAYHGQLKNQAVVRSLGTAQVLDLGDRNGYLDMGTDVGTLIAGLEDFSITTA
                     IYIDEKSNITGNGHFVWAFSTSPACGQYTGKYIAYRVNRQRYALSTGGYANEVAALET
                     GTASIKGKWYQLTYTQQGSKGSLYFDSTLVRSSSNLVYLPKDLGQATNYNWIGRPHFN
                     GDSYLKKAMLSDFRIYNRALGQEEIRELSGQIAGLNAAMNLEILAEAHQNLRLKDTAE
                     ITQDLELPLSLPGDVSVSWTSSHPDVISREGKVTRPAAGSDTATVVLTASLSRGGDTL
                     FKNFEITVLPFFSPEVSVQRDLEALIFSEEENIVKYKLHLPLSGAEGSSLSWYSGNTA
                     YLSHEGDLLQQPAAGEPDVVLNLQVTASKDEARDSRTFIVRIPAELHYYAYLFAYFTG
                     NSGSQEAIRFALSFDGYNYKALNNNNPIISSEEISRMGGVRDPHILRGIDDTTFYMVV
                     TDMKSALGWNSNHGIVMLKSGDLVNWDHTAVDIAEEFEEFSTINRAWAPQTIYDQNAG
                     KYMLYWSMRSGSDIDVIHYAYANEEFNALATVPKVLYHSPDATACIDGDIVYKDGTYY
                     MFYKNEGTGNGIKKAVSEYVNRGWQQYSSRYYQQTSSAVEGSCVFKLYDSDTYILMYD
                     VYTSGRYEFTTSTDLENFRVIGGVSMNFSPRHGTVIPITRNEARRLVEKWGYGFEPIP
                     EDTTSALSPAPAGEELVLWPLPTRDLLHFELNGSAGSQETELQVTGPDGRIWLRELKN
                     DNKPLDCSFLPAGYYILQLRTENSTHRAVFFRLL"
BASE COUNT         2787 a         2618 c         2383 g         3298 t
ORIGIN      
        1 tagagcacat cccttttaag gatggggtcc tgggttcgag ccccaggcgg atcactgaaa
       61 aagaatcgca gatagttgaa aatcaattgt ctgcgatttt tatacttatt ctccggggtt
      121 cctccggcag gggccggtct aaactatctg tccgcaaact actattcggt ttccccctcc
      181 tccatcagcg catcgcttac caaagagccg ggtaggttct ttttggcttt gacgccaagt
      241 ttaatgagtt tgtcggcctg gctgatgagg ttgcctctgc cggattttag tttgtcgaag
      301 gcatcggaat agctgctgcg ggctttgtcc aggtgaccgc ccaagctgga aaggtcgtcg
      361 acaaaaccta ccagtttctc gtagagcaat ttgcctcttt cggcaatggc ctgggcattt
      421 tgattctgat attcgcgctt ccataaatct acgatcagcc tgagggctgc tataaggttg
      481 gtggggctaa tcaggatgat gcgtttttgg taggcataat tccagatttc cgcatcctgc
      541 tgcatggcca ccataaaggc gggttcgttg ggcacgaaca tcataacgaa gtccagcgaa
      601 gaagcgtagt cctggtagga ttttacgctg agttcatcga tgtgtttttt catcgaattg
      661 aggtgttctt tcagggcttt ttgctgttct tcgggatcat cggccgccac atagcgggta
      721 taagccgtaa gcgacacttt ggagtcgata atgattttcc ggtcgtcggg ataggccaca
      781 atcacatcgg gctgcatttt gtttccctgt tcgtttttca ggtaattacc gtcttcatct
      841 ttgagaaatt cctgcacgaa atattctcgg tctttgacca atcccgattt ctccaaaata
      901 ttttccagaa tcatttgacc ccaatcgcct tgggctttgg agctgccttt cagggcattg
      961 gtcaagttat tggcttcttt gctgacctgc tgattgagtt cgaccagcct ttgcacttct
     1021 ttgcccaacg aaaaacgctc cttggcttcg gtgttgtaaa cttcttccac tttcttttta
     1081 aactgatcaa tgttttcgcc caggggttta aggatttgtt ccagattgct ctgattttga
     1141 gcggtaaatt tctgtgtttt ttcttccagt actttattgg cgatattttc aaactcggta
     1201 ttgaattttt tggccagctc ctctatctct tttttttggg tttccaattt ttcgttcaaa
     1261 gccaggttgt tggccctgaa ttcggctaga attttgttgg cgttattgaa ttcttcggtc
     1321 ttgagtcgaa gctcttcttt caactgctcg aagctgaggc ttttttcttt gaggctttcc
     1381 tgggcagaac tcagtctggc cgagtactcc gcctccgatt tggtcagatc gttgatggtc
     1441 gtattcaatg ccgctgtttg ttcttcggcc gtttttagct gtgcggccaa actgttgttt
     1501 ttttcctgtt caatcagcaa agcggtttta ctctcctgag caagtttgtt cagctcttcc
     1561 aattgagctt tgggcactgt ccgggctttt atactcaata ccgaaataag cgaagcaata
     1621 ataccgctcg ctactatggc tgccaataga tataaaaagt tcatatgctt tgtaatttta
     1681 tcagtatcag gtttttgcgg gagctttgta tggaatacta aagctaaagg tgctgccctc
     1741 cttttctttg ctttttatca ggatctttcc gcccaattgc tccacataag ctttagaaat
     1801 agacaatccc aatccggcgc cctcgtgttc acgagtcagg ttgagatcgg cctggacgaa
     1861 acggtcaaaa atagcttctg tgcggtctgc cgaaataccc ctgccggtat ctttgacaaa
     1921 aaagcaaacc tgatcttcct ctatcgaagc tcccagttca acgctgccct cgtcggtata
     1981 tttaatggca tttttaacca ggttgctcag aattctgcgc aaacgataaa catcggttat
     2041 aaagcgaagg tttgagcttt cggaaggagt atttattctg aactgaagat tcttttctac
     2101 agcctgaggt ttgtatgttt ccctgatgaa ttgaacgatt tccgaaatat cggtttcatt
     2161 ttcctgcatt tccaattctc cggactcaat acgggaaatc tcgatgatgt cgtttaaggt
     2221 ttctatcagc ctttttccgc ttaaattaac cgatttaaag tattcatctt tttccttgtc
     2281 gctgagatcc gaagtttgca gtaattgtac aaagcccaaa atgccgctca tcggagtacg
     2341 aatttcgtga ctgatatttg acaagaaagc tgttttgagg cgattgcttt cttctgcctt
     2401 ttccttggcc ttaatcagtt ctttttccct ggcttttcgc tcggtattat caataatgga
     2461 cgcttgtata caaatggctt cgccgttttt atcggtgacc atactggaac taagtaaaac
     2521 atcaaaaaaa ctgccgtcgg ccctttttgc cttgctttca ccttgccatt cacctttttc
     2581 cataaccacc ttcagcagtt cgcgcaactc tgtttccgaa gcccaataac caaacacttc
     2641 cttgttgaga acatcttcta aagcattgta accccaatag tttagacaag ctttgttggc
     2701 atagataatt ttcccctcca tatcaatgat ggctttacct atcatcgaaa gctcgatgga
     2761 acggtctttt ttgatcatct ccttctcggt tttgtgacgt tccagcatac tggtaacaat
     2821 ataagccaga gattccagag ctttgatatt ctcgggctgc caatctctgt atgttttgca
     2881 ttcgttaaaa ccgataaaac cgtaaaaacg gtcttctatc atcaatggta cattcaacaa
     2941 ggaaagaatg cgcagaaaac ggaaaaattt tttggttgct tcgtcgggta tatcttcgat
     3001 gttcctgtat tgaacaatct taccctgttt taagcgatca atgagccagg caacatcttt
     3061 gtactgtatg cgttgaaaaa tgtttttata attctgaaat ccttcggcac accattcgta
     3121 ggtattactg agaatttgat ttttgtcgtc gtattcaaaa atataggttc tgcaaaagtt
     3181 taaagtacgt cctatgtctt ttaaaatggt gttgaaagca gtatcctgtg cataatagcc
     3241 gatggccagt ctggaagttc gcgataataa ggtttctacg gccagtcttt tttcaatgta
     3301 atccccaatc ttttttttct cgttgatatc tcgggtaaca ccatgtaatc caataggttt
     3361 tcccgattta tcacgaacaa acgtcgcatt tatcgatacc caaataaccc ttccgtcttt
     3421 atgaaaatgc tccacttcta cggatctgga tctatttttt tctatttcgg ggtcctgttg
     3481 ctccagcctg aactcctctt ttatcatatc catcactctc tccacctgat caattggaat
     3541 cattttatcc aagggcagag ccaaaaattc ctccggagta aaacccagca ttttttctac
     3601 ggagggagtt atgtagctta gattcaactt caggtccgac aaccaaataa gatcggtcgt
     3661 attttcagct aataatttat atttttcctc actttttttt actttatccc aggcttcttt
     3721 caaagctgtt gtttgtttat ttaccctctt tctcaacaaa aggttgaaaa ttcccacaac
     3781 tacaagggca aacaaagcta agaagaaaat tccggtgatt atttttttgt tgctttcata
     3841 aaaaccatgc ttatacccac ccagaatatc gttatttgac cacttgctta caaaagcctg
     3901 atgttgttcc tcgtcaacct gatccaggag tttgccaaga atggaacaga gttccggcaa
     3961 ctcccgtctg gctgcaaacc ttaaattcca tttgtaatca agaaaaaccg cgtgtttcag
     4021 atttgtcagt cctttatttt gtaccagata attagctgtc atatagtcga caacggcacc
     4081 atcggttata ccataaacaa cttgaagcaa agcttcgtat tcgttgttta cataaacaat
     4141 ttgagccgaa ggataggctt cttcgatgta atacgtgctg gtataccctt ctacgcaggc
     4201 tactttcatg gaattaatct gtcttttgct tacctcggtg ggataattcg cccgggtaat
     4261 cagacctaac tctaccctaa agaaaggttt ggtaaagcgc aggtactctt ctctgagagc
     4321 cgacctttgt ataccaccca caaaatccgt ttctcccttt ttcaatccat ccaacatacc
     4381 cgtccaggtg ggataataaa ctctctcgaa ttctacaccc aacatttgct caaacaagca
     4441 aatataatcg gaaacaaagc ctttgtgtac gccgtcttcc acataattgg caggcggcca
     4501 cgaaggatcc ggagtgtacc ttattttgtc tttgttcaac tgcagccagt tgagttcctc
     4561 tttggtcaac agggaatcga gattgatcgt ttgtgcttgc agatgaaaac ctgccgtaaa
     4621 cataattatc aacaagctcc caataaccgt gtgcctgaat ctgcctatac cttccgaaac
     4681 aaaacgtcga tataaaccga tcaccccctt cttcttaaac ttttttatga acgacatcgg
     4741 caacatgata ttcttatttc cccatttaac tatattcgat aaaaccaaac ataaaaaaca
     4801 gcttaacacc gaatatttta ttggtgcatt ttatattcaa taaaaccaaa cccaaatgta
     4861 taattatttc ttcaatattc atgtataaaa aattattgcg ctatcatttt cggtgagtgt
     4921 aatggttatt aatgctggaa ataagacaca attcctactt atattaacaa tatataacat
     4981 gcaatgcttg tataaaacaa agctatccgt ctattttgtg ggttctaaaa cgatcaaatc
     5041 catggaaaag acaactttct tagtaactta tcttgtcagc tttcttgtgt gcgcaggtgt
     5101 taccctggga ctgattgccc ttatcaacaa aagcctcaag gtgtatttta aacagttgtg
     5161 ccaaaatgct caggttgccg acttgttcat ccggatgaca aaaattgtca ttctgcttgc
     5221 aggcctgggc gcagctctca cttccaacta caccaccaac gaaaaggccg attggctgag
     5281 cctcacctgg aatgtgacgg ctcaggtaaa agcagcgctg gaatccttgt ttattgcctt
     5341 tattattctg gccctggcct ttttggtgct gcatgtctgc acgcgaattt gtaagaaatg
     5401 acttactgga caagtactca aatcgcactg atttattttg gaggaatcgc cctggcgatt
     5461 ctttcagccg tacttgtttt gctctatcta aagcattgtc gcagagcctt tttagtcatt
     5521 tggacaaaat ttcgcaaaac aagcatttgg ggttttaaga gttcactgat agtggctgct
     5581 ttgctgggtg ctttgtcggt gaattacctg gactgctcgg gcgaatacga atacctgaag
     5641 gaatccggct atttggtcct gaccaagggc ctggaacaaa tcggaacagc ccttgtattg
     5701 ctctcgataa cgacgggagc ctggcttttg gtttttataa tccgtatgct tgtaaacagg
     5761 cgttccaaat ccgaaaagca aagttaaaaa acgcaatcag cgaaaaaatt atatttcttt
     5821 gatatatatc cgtctggtct tgtgatgacg cggaccgaat tccgaccaca aagcctgaat
     5881 gcggctgttg gtttccagtt cgggattggt ctcggccaaa cgataaccgt ttttttggaa
     5941 tttggggagc atatcggtaa atatcagcga attgagccct ttactttggt attccggatg
     6001 tatgcccatc aggtaaaaat ctaccagatc gtttttgcga atggctttga gcatatgtat
     6061 aaagccaaaa ggtaaaagtt tgcctttggc tttttggaag gctttactca tggagggaat
     6121 ggcaatgccc aggcccacca cccggtcttc gggtgtaacc acaagactta ccagttcggg
     6181 acgcacaaac gagaggtaaa gttttatata gtaatccacc tgcttttcgt tcaaaggcac
     6241 ataaccgtac aaaacatcgt aactgtcgtt gagcagctga aaaattttct tgccgtaacg
     6301 cttcgccagc tctctcgtac ttttgcaact aacaactttg agatcaaatt tttgagccaa
     6361 aagttcggca aaacggaaat aacgctcgct gagctcttcc ggaacgtcaa tttgaaactc
     6421 cacccaatct acttctttgc gaaaacccag ttgttccagg tgttgcggat agtagggata
     6481 attgtacaga gtggcaaaag tgcccaattg gtcgaaacca tgtataagca tgccttcgtt
     6541 gtcgaaatca caaaaaccca tagggccctt gatggcttcc atacctcttt gtctggccca
     6601 atcggcggcg gcacccatca gagcttcggt cacttcggga ttgtctacca catcaaacca
     6661 gccgaaacga acatgctttt cgtcaaagcg cttattggct tccttattga gaattacagc
     6721 tatgcggccc aatagggttt ttccttcctt agctacaaaa caggccactt cgcaaaactc
     6781 cagagccgga tttttagccg gattgaacgt gtttaactca tcaaaaatga gtggtggaac
     6841 gaaataagga tgatccttgt agagtttgat cggaaagcta acaaactctc tgagttcctt
     6901 tttggtgcta atctttttaa tttccatctg cctgccatta gtgcaaacta tgcgggcaca
     6961 aaaatagaaa taaaaatcaa atattgaact ctgaactcat agtaaatcga atatcgggaa
     7021 aagtctcgcg gctgttgttg agcatccagt cgctttcggc cataaataca gccctattgt
     7081 ttttgtcgaa aactatatga gctgctttgc gtttaataaa ttcttccagc tgcgcccggt
     7141 tgtcggaacc aaaccaacag gctttggcgt aaggcaggag ttgaaagcgg catttggcgc
     7201 cgtattcttt ctcgagcctg aattgaatca cgtcaaattg caattctccc acagttccga
     7261 ttattttgcg ttttccctgc tcctgtacaa acaactgcgc caatccctcg tcggtaagtt
     7321 gtctcaggcc tttttcgaat tgtttggctt tcatgggatc ggcatttatg acttccttca
     7381 gaatttcggg cgcaaaattg ggaattccct taaaagtcag gatttcgcct tcggtaaggg
     7441 catcgcctat cttgaggttg ccgttgtcgt acaaaccgat cacatctccc ggataagctt
     7501 cgtctaccgt acttttgtta ttagccataa acgtggccgg agccgaaaaa cgcatattct
     7561 tgtccaagcg gcaatggtaa taaattttgt tgcgctcaaa gcggcccgaa ctgatgcgca
     7621 aaaaggccat gcgattgcgg tggttgggat cgaggttagc gtgaatctta aaaacaaaac
     7681 cgctgaattt ttcttcttcg ggctgtacaa gacgttctgc cgttttcagg gcagcaggtc
     7741 cgggagcgat ttcaacgaaa ctgtcgaaca actccggtac tccgaaactg ttcaatgcac
     7801 tgccgaaaaa gaccggagcc aggtaggcct cacgataaag cgaaagattg aaactctcga
     7861 aaagcccggg tataagctcc atatcttcct tgagtttatt tatcaaatcc tgtccgacta
     7921 tacggcgcaa ttccgtattg ttcaggtctt tggtattgag caactccgtc gatttgagcg
     7981 ttttttgcgg gtcgtacaac cgaacatctt tccggtacag gtggtacacg cctttgaaac
     8041 ggcttccctc gccaataggc caggtaaaag gacaggtggc aatcttgagt tcctcttcga
     8101 gttcgctcag taaatcgaag gggtctttgc cctcgcggtc catcttgttg atgaaaacca
     8161 ttacgggaat agcccgcata cggcatacct ccatcaaccg acgggtttgt tcctccactc
     8221 cttttacgct gtctataacc aaaaccacgc tgtctaccgc agtaagcgtc cggtaagtat
     8281 cttcggcaaa atccttatga ccgggggtat cgaggatgtt caccctcttg cctttgtaat
     8341 cgagagtcat caccgaagtc gccaccgaaa tgcctctttg cctttcgatt tccaaaaagt
     8401 cggaagtagc tgttttacgg attttattgc tctttacggc cccggccact tgtatggctc
     8461 cgcccataag caggagtttc tcggtcagag tggtcttacc tgcgtcaggg tgagagataa
     8521 tggcaaaggt gcgcctgcgt ttgatttctg ttttaatatc cacgtgctat aagtcttggg
     8581 ctgcctcaca acaacctgaa aaaaaccgcc cggtgtgtac tattttccgt tctaagctgc
     8641 aaaatgtagt agccggcggg caaaaaggaa caatccaggg gtttgttgtc gtttttaagt
     8701 tcccgcagcc atatccggcc gtccggacct gtcacctgca actccgtttc ttgcgacccg
     8761 gcactgccgt tcaattcgaa gtgcaacaga tccctggtag gcaaaggcca caaaaccaat
     8821 tcttccccgg ccggagccgg ggacagggca ctggtagtgt cttcgggaat gggttcaaag
     8881 ccatagcccc atttttccac cagccggcgg gcttcgttgc gggtaatcgg aatcaccgta
     8941 ccgtgacgcg gtgaaaaatt catggaaaca cctccgataa cacggaaatt ttccaggtcg
     9001 gtactggtgg taaattcata gcggccgctg gtatagacat cgtacattaa tatataggtg
     9061 tccgagtcgt acaatttaaa cacacaagag ccttctacag ccgacgaggt ttgctgataa
     9121 tatctgctgc tgtattgttg ccagcctctg tttacgtact ccgaaaccgc ttttttgatg
     9181 ccgttgcccg tgccttcatt tttgtaaaac atgtagtagg tgccgtcttt ataaacgata
     9241 tcgccatcga tgcaagccgt ggcatcgggg ctgtggtaca agaccttggg aaccgtggcc
     9301 aaagcattga attcttcgtt ggcgtaggcg taatggatca catcaatatc cgaacccgaa
     9361 cgcatggacc agtagagcat atatttaccg gcgttctgat cgtagatggt ttgtggagcc
     9421 caggcccggt tgatggtgct gaattcctca aattcttcgg caatatccac agccgtatga
     9481 tcccagttga ccaagtctcc ggatttgagc atcactatac cgtggttgct gttccaaccc
     9541 agggcagatt tcatatcggt gactaccatg taaaaagtag tatcgtctat gcctcgcaga
     9601 atatgcggat cgcgcacccc tcccattcgg ctgatttctt cggaactgat gatcggattg
     9661 ttgttgttga gcgccttgta gttataaccg tcaaaactca gagcaaagcg tatggcttcc
     9721 tggcttccgg aattgcccgt aaaataggca aaaagatagg catagtaatg gagttcggcc
     9781 ggaatgcgga cgataaaagt ccggctgtcc cgggcttcgt ccttactggc cgtcacctgc
     9841 agattcaaaa cgacatcggg ttcaccggcc gcgggttgtt gcaacaaatc gccctcatga
     9901 ctaaggtagg ccgtattgcc cgaataccag ctcagactgc tgccttcggc gcccgacaaa
     9961 ggcaaatgca gtttgtattt gacaatgttt tcttcttccg aaaaaatcaa agcctccaaa
    10021 tcgcgctgaa cagaaacctc aggactgaaa aaaggcagaa ccgtgatttc aaagttttta
    10081 aacaaagtgt cgccgcctct gctcaggctt gccgtcagaa cgacagtggc ggtgtcgcta
    10141 ccggctgccg gacgcgtaac cttgccttcc cggctgatga cgtcgggatg ggaagaagtc
    10201 caggaaacac tcacgtctcc gggaagactc agcggaagtt ccaggtcttg tgttatttcg
    10261 gcggtatctt taagcctgag gttttgatga gcttcggcca gtatttccaa attcatggcc
    10321 gcattcaaac cggcaatttg cccggaaagt tccctgattt cttcctgacc aagggcgcga
    10381 ttgtatattc taaaatcgct gagcatggcc ttttttaaat atgaatcacc gttaaagtgc
    10441 ggccggccta tccagttgta gtttgtggcc tgcccaaggt ctttgggcaa ataaaccaga
    10501 ttggacgaag accttactaa ggtactgtcg aaatacaggc tgcctttcga accctgctgt
    10561 gtataagtca attgatacca cttccctttg atcgatgccg taccggtttc cagagcagcc
    10621 acttcgttgg cgtagccccc ggtagacaag gcataacgct ggcggttcac cctgtaggct
    10681 atgtatttcc ccgtatattg cccacaggct gggctggtcg aaaaagccca gacaaaatga
    10741 ccgttgcctg tgatgttgct tttttcatca atgtaaatcg cggtagtgat gctgaaatct
    10801 tccaatcccg caatgagcgt tcctacgtcc gtgcccatgt ccaggtaacc gttccggtcg
    10861 cccagatcca agacctgtgc cgttcccaaa ctgcgaacga cggcctgatt cttcagttgg
    10921 ccatgatagg cgttaccgct ctgatcggac accgtaaagc cctcgtttaa aaaagtataa
    10981 tgtacctgga gactggagtc ctgaatcgcc ttcgccgccg gaggcagaag catcaaaaat
    11041 gccgtaagaa taaaattagc cgctttcata agacttagtt gaaatt
//
LOCUS       DAOSGL010000037        10838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5114_length_10838_cov_4.596865, whole genome shotgun sequence.
ACCESSION   DAOSGL010000037 DAOSGL010000000
VERSION     DAOSGL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10838
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5114_length_10838_cov_4.596865"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PKY68_02240"
     CDS             <1..483
                     /locus_tag="PKY68_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOH23892.1"
                     /translation="DPATASTRESAGLILDSMAVKEKFVKEDPFEKGPRKALNLGHTI
                     GHAFESLSHKRNIALLHGHAVALGLVCELYLSNKLAGFPEKELKACANRVKQTYSAFP
                     LQKSDYSELIGFMQHDKKNRDQHIRFCLLKDIGQTLPDTTATPEQIVESLDYYCENLL
                     "
     gene            complement(597..672)
                     /locus_tag="PKY68_02245"
     tRNA            complement(597..672)
                     /locus_tag="PKY68_02245"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Pro,seq:ggg)
     gene            845..1603
                     /locus_tag="PKY68_02250"
     CDS             845..1603
                     /locus_tag="PKY68_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOH23893.1"
                     /translation="MQQALEDVLSRFSPILLEEMGKVKLMNRIDTKYIARVDRLIPLL
                     EEMTTGYYAQEINGKRIARYHTLYMDTPDADMYLAHHNGRKVRQKIRIRDYEESNLSF
                     FEIKNKNNKGRTKKRRIKLENTDFHRNEQVLELMDKYAEYKIDGLIPQLENFFNRITL
                     VNKDKTERLTIDQGLRFHNLTTNAWSDLSKIIIIELKQDGNCRSHAKEILSSLRIHPN
                     RISKYCIGSVLTNPELKNNRFKRKLTLINKIIND"
     gene            1605..2306
                     /locus_tag="PKY68_02255"
     CDS             1605..2306
                     /locus_tag="PKY68_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOH23894.1"
                     /translation="MDEFAEMLKAEELLEAQSMVTPIFNSHDLWELLFRFLFNFVVIG
                     IIIHFFYYRKSRRVDYYFTFIIISVSIFFLIYLLGSVKLKIGFALGIFAIFGIIRYRT
                     EQVPIREMTYLFTITAISVINALSIQMSYTQMVATNLVIILVIWGLETSRWLKHTNSK
                     MVLYDRIQLILPEREKELIDDLKKRTGLDIIRVEVGHIDFSRDVAFIKVYYESDKVEI
                     NATDQITRFPKWSNQ"
     gene            2317..2994
                     /locus_tag="PKY68_02260"
     CDS             2317..2994
                     /locus_tag="PKY68_02260"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOH23895.1"
                     /translation="MNIKSIFTALIAFCCLLSLRAQENTDDWGGQVSVEISKKIIKKL
                     TLSFEEEFRLHKNFSRADRFSHSLDLTYKPFKFLKGGVSYNLLNYYDLQDGWDYRHRY
                     NIHLTGDKSFGRFNVALRERLQGTYRPQALIDHNPKWYLRSRVKVEYDIRKSHFEPFA
                     SAEMYYSLNDPDPANRGINRWRFIAGSKYNINKKNNLQLYYRYTNYADQDDDNGHMIC
                     IGYSVDI"
     gene            3006..4913
                     /locus_tag="PKY68_02265"
     CDS             3006..4913
                     /locus_tag="PKY68_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOH23896.1"
                     /translation="MKKTSTFGLLILLIFWMACCKPDGPDSPDVPDKPDTPDNPTDTL
                     NTDTLVIDSTAVSIFYTDTGIVITNPLENEILVQQSNGHISVRSQAAGMKFVLSGAHP
                     DASFSIASDSSFSLILDSLNLTSLVGPAIYIQSRERTLVYLNDSTENFLNDAITYSSE
                     NDADTRACLYAKGGLIFGGKGKLTIQSKSKHALCSDRFIRIQEGEIMVETAARDGIHV
                     SDYFVMDQGRLHINSLSDGIECDAGFIRINGGELMIHSEDEGIITSYLGPDTAIKAGI
                     VIEQGSITVTTTDEKSMGIDAAGDLVVYGGTLIVELEGNASKALRSKGNMFLLGGCFD
                     LTTKGNAAWDEKDADFSSAAGIKCDGNLQIAGAETSINILSTGKGGKGINTEGQILIE
                     EGSIQVKTTGVRDVYGTYSSNAKGIKCDADLTISGGKIKVESTGSSGSDGIDSDGSLS
                     IDGGEIEVLAYDDCIKSLRNMTLNNGNIYCYSTHNDALDTKASLTVNGGFIIASGAAG
                     SKNGVNCDGIFKINGGCLLATGGKNTSPSSTSAQNSVIYNGLFNSGECIHLQTAEGKP
                     VMTYQFPRTYSVLKLTYSASDLTKGNYSLYKGGEVSGGENVHGFILGGSYTPGTWLMD
                     FSISGTVTKVN"
     gene            complement(4917..8300)
                     /locus_tag="PKY68_02270"
     CDS             complement(4917..8300)
                     /locus_tag="PKY68_02270"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOH23897.1"
                     /translation="MKKGLILFLYLLFSFTVSAQLKINELMPKNISAVRDEYTNYSMW
                     VELYNASDREVELAGYFFSDDSKSPRKWNPDAKTIPAGGFEILWFEREDRSGHASFKL
                     KPEGGVLYLFNRSGLVVDKVHYPPQYRNVSYGRLIDGEDSWSFFATASPGASNSEGRA
                     AIARCPNPEFKLNGGFYDGPRYFGFNTPSRGDIYYTLDGSEPNINSTKYRPGASVYIS
                     KTTVLRAICLAEDCLPSDLLTTTYFIGERNFDLPVVSLVADPVHLWDNMYGIYVDGIN
                     GIPGNGQSQPKNYNQDWSRPANFELIDTSGVLQINQELDIAIAGGWTRAHGLKSLKIQ
                     PRTKFGNNHLDYDFFPVSKPGQLYKDILLRNSGNDNGRTQMRDGFTVSILQNRIDLEY
                     MAYEPAVLFINGRYYGIENLRERSNKDLIYCNYGLEEEDIILDGVPDVDLFPLTPEYA
                     ELHQFIEELDMTSDTAFLAVCRRIDMENYLHYQLFEIYVGNTDWPHNNFKLWKRKVDG
                     KWRWIIFDLDFGFNGVNGPTHNTLAHALGEGNSWGQWSTMMFRRLMLNTEFRQRFIDN
                     FCVELSKTLEPERVIAILDSMAPKIANEIVYHKQRWGSSADFYNDLESMRSFARQRPN
                     NILNQLSGRYLGGVSNGLIRLSANHPQASYYLNRAYIQDAEINLRYFLQQNINIRAAR
                     VQGMQFSHWEVNKGGGIDQLIANGANWRYYYTSHSPGEAWNSPAYNDQSWSQGPAQFG
                     YGGKGEVTVLDYGNYVNKYPTAYFRKEIQIDDLANKNSFLLSIFVDDGAVIYVNGQEL
                     ARLNMPAGTITYNTYASSANNGLNAEYDVPLNMLKEGSNLIAVEVHQVSASSSDLIFD
                     LAMSCVDMNGNIQTLTEEVYSGIFDSELSLRAIYEEDPHAADIRPTVLINEIVASNNR
                     IADEYGEKDDYIELYNYGEEDLNIAGWYLSDLPMYPFLGRIAATDSAQTVLPAKSWIC
                     LWADGTPEQGPLHLNFKLNLLGESVSLSKKDEFGRLMLIDSVSYPPLDRNMSYSRIPD
                     GGSVWEIQEPSFGASNLLAGLKFNIEEREIEVYPSLVKSSISVRNAQGSRFSLFDFAG
                     RPVCAGFCVDPVEIIDLSGLQPGLYILKVDGQVFKLIKQ"
     gene            complement(8495..>10838)
                     /locus_tag="PKY68_02275"
     CDS             complement(8495..>10838)
                     /locus_tag="PKY68_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23898.1"
                     /translation="TKIYGGTIDNVFGGSNTNGAIGGTVTVNVNDAAENSCALLAGNI
                     YGGGNMAPYSNSGNYPQVNITSGTIEGNVYGGGLGQTAAVTANPRVNLTGGIIGGDVF
                     GGGDAAPVTGSPIVSAVTGSSGTRLFGGGKGATATVTGNTTVTVSGGTFTDIFGGGDA
                     ALVTGNTTLTLSGGTMDGAVYGGGNNAGVNGNTGITISNGTISDGVYGGCNVTGTIGG
                     NVNLMLTGGTIGTAGSKSYIHGGGFGKNTTVSGNVDLYVGTSGQTTPGVTIYGDVYGG
                     SALGSVNTGTSNTTKVYLYKGKIYGDAYGGGLGDSETAALVNGNVEVLLNGAAFGISY
                     TGEGDDRIPVSGRIFGCNNINGTPKGTVKVTVLATTPIGGGAHTLGTYEVQGVYGGGN
                     LAAYDPTNSNTPTYVEVDGCDDVSIEYVYGGGNAAPVPATEVMIEGAFEIDYVFGGGN
                     GKDKVGGMENPGADVGLKSGGISYGTGNKIGTTLVTVLGGTIHNVFGGSNTKGDVPNG
                     TNVILGDENPQICAFNVDNVYGGGNEAYLSGSSEIILNCIEGLDEIYGGSRMADIDDD
                     VNLHILSGTFGKVFGGNEESGAISGSITVTIEESGCLPIKIGELYGGGNRAPYSVYGY
                     EVDGSGKVLRDAYGKPVLKTSGAKLYADPVINIISATEIGSVFGGGLGRAAVIYGSPE
                     ININMVEGTLNGVYRYVAGSSQPGNKPYDPEQPEYIGPNLELGKIGNVYGGGNEAAVY
                     GNTTVNVATETGKSAYIINNVYGGGNQANVSGTTKVTIGK"
BASE COUNT         2912 a         2651 c         2431 g         2844 t
ORIGIN      
        1 gatccggcca cggcctccac tcgtgaaagt gccgggctta ttctggattc catggcggtc
       61 aaagaaaaat ttgtcaaaga ggatcctttc gaaaagggcc cgcgtaaagc cctaaacttg
      121 ggacatacca taggacatgc attcgagagt ctttcccata aacgaaacat agccttgctt
      181 catggtcacg ctgtggccct gggtttagtc tgcgagcttt atctttcaaa taaactggcc
      241 ggtttccccg aaaaagaact gaaagcatgt gcgaatcgtg taaaacaaac ctattccgcc
      301 ttcccactgc aaaagtctga ttattcggag ctaatcgggt ttatgcaaca cgacaaaaag
      361 aaccgcgatc aacacatccg tttctgcctg ttgaaagata tcggccagac cctgccggac
      421 acaacagcta ctccggaaca aatcgtagaa agtctggatt attactgcga aaacttgctg
      481 taaaagaatc aaaacacggg atcaaacctt gcgccgcatt gattccgttt ctttttaaaa
      541 ccaaaagaaa taaaaaagcg ttccgggaat caatgaatcc cggaacgctt tttcagtcgg
      601 ggtgactaga ttcgaactag cgaccactcg ccccccagac gagtactcta aaccggactg
      661 agctacaccc cgaaagcggc cgcaaatgta agacgaattt ttggattgaa caaactccgc
      721 ccccttttta tttatgacaa tttagtcgaa tattatacca aaatcaactc ataatattga
      781 tagtacttgg tgacaccgtg cattttagtt acttttgtaa gctaaattaa taccgtaaaa
      841 acaaatgcaa caggcgcttg aggatgtatt aagtcggttt tcccctattt tgctcgaaga
      901 aatgggaaag gtaaagctga tgaaccgtat cgacaccaaa tatattgcac gtgtcgaccg
      961 cttgattcct ttgcttgaag agatgacgac aggatattat gctcaggaga tcaacggcaa
     1021 acgcattgca cgataccaca ccctgtacat ggatacaccc gatgccgaca tgtacctggc
     1081 tcatcacaac ggacgtaaag taaggcaaaa aatacgtatc cgcgattacg aagaatccaa
     1141 ccttagtttt tttgagatta aaaacaaaaa caataaaggg cggaccaaaa agagaaggat
     1201 aaaactcgaa aacaccgatt tccatcgcaa cgaacaggtg ttggagctaa tggataaata
     1261 cgcggaatat aagatagacg gcctgattcc gcaactggaa aattttttca atcgcattac
     1321 tttggtcaat aaagataaga cggaacgttt gaccatagac cagggcttga ggtttcataa
     1381 tttaactacc aatgcctgga gcgacttatc taagatcata ataattgagc tgaaacaaga
     1441 cgggaattgc cgttcacacg ccaaggaaat attgtcttcg ctgcgcattc atcccaacag
     1501 aatcagcaag tattgcatag gttcggtact gaccaatccg gaattgaaaa acaacagatt
     1561 caaacgtaaa ctcaccctaa taaacaaaat cataaacgac taatatggac gaatttgcag
     1621 aaatgttaaa agcggaagaa ttgctcgagg cccaatctat ggttactcct attttcaatt
     1681 ctcacgattt gtgggaattg ttgttccgct tccttttcaa ctttgtagtg ataggaatta
     1741 ttattcattt tttctactac agaaaaagtc ggagggtaga ttattacttt acttttatta
     1801 tcatcagcgt cagcatattc ttcctaatct acctcttagg tagcgttaag ctgaagatag
     1861 gatttgcctt gggtattttt gccattttcg gcattatacg ctatcgcacc gaacaagtgc
     1921 cgatacggga gatgacttat ttgtttacca taactgccat ttctgttatt aatgccctgt
     1981 ctatacagat gagttatacg caaatggtgg ccaccaacct cgtcattatt ttggttatct
     2041 gggggctgga aacctcacgc tggctcaaac acaccaacag caaaatggtg ctttacgaca
     2101 gaattcagct gattttgccc gaaagagaaa aagaactgat tgatgatttg aaaaaacgta
     2161 cggggcttga tattatcagg gtagaagtgg gtcatatcga cttttcgcgc gatgtggctt
     2221 ttataaaagt ttattacgaa tcggataagg tagaaataaa cgctaccgac cagattacgc
     2281 gtttccccaa gtggtctaac caataaatac attccgatga atattaaaag tatcttcacg
     2341 gcactcattg ccttttgttg cctgctttcc cttcgggcgc aggaaaatac cgacgattgg
     2401 ggcggccagg tgtcggtaga aatttcaaag aagataatta aaaaactaac gctgagcttc
     2461 gaagaagaat tccggctaca caagaatttc agccgggccg accgtttttc gcacagcctg
     2521 gacctcactt ataaaccctt taaatttttg aaaggtggcg taagctataa tttattgaac
     2581 tactacgatc tccaggatgg ttgggattac aggcaccgtt acaatattca cctgacaggc
     2641 gataaatctt tcggacgttt caatgtcgct ttgcgcgaac gcctgcaagg cacttaccga
     2701 cctcaagcct taatcgatca caatccaaaa tggtatctgc gcagccgggt taaggtagaa
     2761 tacgacatca ggaagtctca ttttgaaccc tttgcttctg cagaaatgta ctacagcctg
     2821 aacgatcccg atcctgccaa caggggcatc aaccgctggc gctttattgc aggcagcaaa
     2881 tacaacataa acaagaagaa caacttgcaa ttatattacc gctacaccaa ttacgccgac
     2941 caggacgacg ataacggcca tatgatctgc atcggatatt ccgtcgatat ataaattaaa
     3001 aagtgatgaa aaaaacttcg acttttggct tgctaatctt attgattttt tggatggctt
     3061 gctgcaaacc cgatgggccc gatagccccg atgttccgga taaacccgat actccggaca
     3121 accccaccga tactttgaat acagatactt tggtgatcga ttccacagct gtgtcgattt
     3181 tttataccga tacgggcata gttattacca atccgctgga aaacgaaata ctcgtccaac
     3241 aaagcaacgg acacatcagt gttcggtctc aggccgccgg tatgaaattt gttctgtccg
     3301 gagctcatcc ggatgcctcg ttcagtatcg ccagcgacag cagcttcagt ctgatcctgg
     3361 atagtcttaa cctgacttct ttagttggcc cggccattta tattcagagt agggaaagga
     3421 ccctggttta tcttaacgac agtacggaaa acttcctgaa cgacgccatc acctattcca
     3481 gcgagaatga tgccgatacc cgtgcttgtt tatatgctaa aggaggcctg atttttggcg
     3541 gtaaagggaa attaaccatc caaagtaaaa gcaaacatgc gctttgttcc gatagattta
     3601 ttcgtattca ggaaggagaa ataatggttg aaacggccgc tcgcgacggc attcacgtga
     3661 gcgattattt tgtcatggac cagggcaggc tgcatatcaa cagtctgagc gacggtatag
     3721 aatgcgatgc cggttttatt cggattaacg ggggtgagct catgatccat tcagaggacg
     3781 aaggcataat aacttcctac ttagggccgg acacggccat taaagccggt attgtgatag
     3841 agcaaggaag tataaccgta acaaccacgg acgaaaaaag catgggtatc gatgccgccg
     3901 gtgatttggt cgtttatgga ggcactctga tagtagagct ggagggcaat gcttccaaag
     3961 ccttgagaag taagggaaac atgtttctcc tcggaggttg ttttgatttg acgaccaagg
     4021 gaaacgccgc ctgggatgag aaagatgccg acttctcttc ggcagcggga atcaaatgcg
     4081 acggcaacct gcagatcgcg ggagcagaaa caagcatcaa catactgagc accggcaaag
     4141 gcggcaaagg catcaatacg gaagggcaaa ttcttattga agaaggcagc attcaagtca
     4201 aaactacagg cgttcgagat gtttacggca catactcctc taacgccaag ggtataaaat
     4261 gcgatgccga cctgactatt tccggcggta aaatcaaagt agaaagcacc ggaagttccg
     4321 gcagcgacgg catcgattcc gacggcagcc tcagtatcga cggaggagaa atcgaagtac
     4381 tggcctacga cgactgcatc aaatcgctcc gcaacatgac cctgaacaac ggcaatattt
     4441 attgttacag cacacacaac gatgcgctgg acaccaaagc ttcgttgact gtgaacggag
     4501 gttttattat agcttcggga gccgcaggca gcaagaacgg agtaaactgc gacggtattt
     4561 tcaaaatcaa cggaggctgc ctgctggcca ccggcggcaa aaacaccagc cccagcagca
     4621 cttccgctca aaactctgtc atatacaacg gactattcaa ttcgggagaa tgcattcatc
     4681 tgcagacggc cgaaggtaaa ccggtaatga cctaccaatt cccccgcact tattcggtac
     4741 tgaaactgac ctacagtgct tcggatctga ccaaaggcaa ctattccctc tacaaaggag
     4801 gagaagtcag cggaggcgaa aatgtacacg gttttatatt gggcggcagc tatacaccag
     4861 gaacttggtt gatggatttc agtataagcg gcactgttac caaagtgaac tgatctttat
     4921 tgcttgatta gcttaaagac ttgtccatcg acttttagaa tatagagccc gggctgcaaa
     4981 cccgacagat cgatgatttc tacaggatca acacagaacc cggcacagac cggtctgccg
     5041 gcgaagtcga agaggctgaa ccgtgaacct tgagcgttcc tgacgctgat gctgctcttc
     5101 actaaactgg gataaacctc aatttccctt tcctctatat tgaatttcag gcctgccaac
     5161 aggttggaag cgccaaaact gggctcttga atttcccaga cgctgccgcc gtcgggaata
     5221 cgggaataac tcatgttgcg gtccaggggt ggataactta ccgaatcgat caacatcaga
     5281 cggccaaact cgtccttttt cgataaggaa acgctctcac ccagaaggtt gagcttgaaa
     5341 ttcagatgca aaggtccctg ttccggtgtg ccatcggccc aaagacaaat ccacgatttg
     5401 gcaggcagta cggtttgagc ggaatctgtg gcggctattc tgcccaaaaa cgggtacata
     5461 ggaagatcgc tcagatacca gccggcgatg ttcaggtctt cctctccata attatagagt
     5521 tcaatgtaat cgtccttttc tccgtattcg tcggcaatcc ggttgttgct ggccactatt
     5581 tcgttgataa gtaccgtagg acggatgtcc gcagcgtggg gatcttcctc gtaaattgcc
     5641 ctgagcgaca attcggaatc aaagattccg ctgtaaactt cttccgtcag tgtttggatg
     5701 tttccgttca tatccacaca actcatggcg agatcaaaga tcagatcgga gctgctggct
     5761 gagacctgat gcacttctac ggcaatcagg ttgcttcctt ctttcaacat attgagcgga
     5821 acgtcgtatt cggcgttcag tccgttgttg gccgaagaag cataggtgtt gtaggtgatg
     5881 gtgcctgccg gcatattgag tctggccagt tcctggccgt ttacgtatat aacggccccg
     5941 tcgtcgacaa aaatactgag aagaaaactg tttttgttgg ccagatcgtc gatttgaatt
     6001 tctttcctga aatatgcagt ggggtatttg ttaacgtaat tgccgtaatc caggacagtg
     6061 acttctcctt tgccgccgta gccaaactgg gcaggcccct gcgaccagga ctggtcgttg
     6121 taagccgggc tgttccaggc ttcgcccgga gaatggctgg tgtaatagta acgccagttc
     6181 gctccattgg caatcaattg atcaatgcca cctcccttgt tgacttccca atgcgaaaat
     6241 tgcatgccct gcacgcgggc agccctgatg tttatgtttt gttgcagaaa atacctcagg
     6301 tttatctcgg catcctgtat ataagcccga ttcaggtaat agctcgcctg cgggtggttg
     6361 gcgctcaatc tgatcagacc gttggaaacg ccgcccagat atctgccgct aagttggttt
     6421 agtatattat tcgggcgttg ccgggcaaaa gaacgcatcg attccaaatc gttgtagaaa
     6481 tcggccgaag atccccagcg ctgtttatga taaacgattt cattggcaat tttaggagcc
     6541 atactgtcca aaatggcaat aacacgttcg ggttcaagtg ttttggataa ctccacacaa
     6601 aaattgtcta taaaacgctg acgaaattcg gtgtttaaca ttaagcgtct aaacatcata
     6661 gtcgaccact gtccccaaga attaccttcg cccaaggcgt gggccagggt attatgcgta
     6721 ggcccgttta cgccgttgaa cccgaaatcg aggtcaaata ttatccaacg ccatttgccg
     6781 tccactttgc gtttccataa cttgaagttg ttgtgtggcc agtcggtatt gcccacataa
     6841 atctcaaaaa gctggtagtg gaggtaattt tccatatcga tacgccggca gacagccaga
     6901 aaagcggtat cggatgtcat atccaattct tcgataaatt gatgcaattc tgcgtattcc
     6961 ggtgtcagcg ggaaaagatc cacatccggc accccgtcca gaattatatc ttcctcttcc
     7021 agaccgtaat tacaataaat caggtctttg ttgcttcttt ctctgaggtt ctctatgccg
     7081 taataccttc cgttaataaa caataccgca ggctcatagg ccatgtattc gagatcaatt
     7141 ctgttttgga gtatggaaac agtgaatccg tcgcgcatct gagtacggcc gttgtcgtta
     7201 cctgaattac gcaacaggat gtccttgtac aattggccgg gtttgctgac agggaaaaag
     7261 tcgtagtcca ggtgattgtt tccaaacttt gtcctgggtt gaattttcaa ggatttgagt
     7321 ccgtgggctc tggtccagcc tcctgcaatg gcaatatcga gctcctgatt gatttgcaat
     7381 actccgctgg tatcgatcaa ttcgaaattg gcgggccttg accaatcctg attgtagttt
     7441 ttgggctgac tttgtccgtt accgggaatg ccgttgatac cgtccacgta aatgccgtac
     7501 atattatccc aaagatgcac aggatcggcc accagcgaca ccacaggcaa atcgaaattt
     7561 ctctcgccta taaaataggt ggtcgtcagc aaatcgcttg gcaagcaatc ttctgccaaa
     7621 cagattgccc tcaaaaccgt agtcttggaa atatataccg aagcgccggg ccgatatttt
     7681 gttgagttta tgttcggctc ggagccatcc agggtataat aaatgtcgcc cctcgatggg
     7741 gtgttaaaac caaaatagcg cggcccgtcg taaaaccctc cgttaagttt aaattcgggg
     7801 ttggggcaac gagctattgc agctcttcct tcggaattgg aggccccggg cgaagccgtg
     7861 gcaaaaaaac tccagctatc ttccccatct atgaggcgcc cgtaagagac gttccggtat
     7921 tgcggaggat aatgcacttt gtcaaccacc aacccgctgc gattgaataa ataaagtacg
     7981 ccgccttcgg gtttcagttt aaaactagcg tgtccgctgc ggtcttctct ttcgaaccaa
     8041 agtatttcga accctccggc gggtatggtc ttagcatcgg gattccattt gcggggagac
     8101 ttggaatcgt cgctaaaaaa atatccggcc aattccacct ccctgtccga agcattatac
     8161 agttcaaccc acatactgta attggtatac tcatctctga cagccgaaat attcttaggc
     8221 atcaactcat tgatttttag ctgtgccgat actgtaaaag aaaacagcag gtaaagaaaa
     8281 agtataagac cttttttcat acaaaagagg ttttttcccg tgtatttcaa accttcaaac
     8341 aagattggat ttttaataca aaggctacaa atatacatat tttaggcggt ccagccccaa
     8401 cctctggaca gcctatacca aaaatatcaa actttgtgag atttcgctgc tgcaattgag
     8461 cggcaggctg ccggagtact acctgcccta agaatcattt tcctatagtc accttggtgg
     8521 tgcctgaaac attggcttga ttaccgccgc catatacgtt gttgatgatg taggcgctct
     8581 tgcccgtttc ggtggcaacg ttaacggtgg tgttaccgta aactgccgcc tcatttcctc
     8641 ctccgtaaac attgcctatc ttaccaagtt caagattggg gcctatgtat tcaggttgtt
     8701 caggatcata gggtttgttt ccgggttggg aactgcctgc aacataacga taaacgccgt
     8761 tcaatgtacc ttccaccatg tttatgttga tttcgggact gccgtaaatt acagcagctc
     8821 ttccgagccc tcctccgaat acactgccta tctccgtagc agatatgatg tttataacgg
     8881 gatcggcgta taattttgca ccggaagtct taagtacagg cttgccgtaa gcgtctctta
     8941 acaccttccc ggatccgtca acctcataac cgtaaaccga atagggagct ctgttgccgc
     9001 ctccgtaaag ttcgcctatc ttaataggca gacaaccgct ctcctcaatc gttaccgtaa
     9061 tagagcccga tattgctccg gattcttcgt ttccgccgaa taccttaccg aaggtgccgc
     9121 tcaaaatatg cagattcaca tcatcatcaa tatcggccat tctcgagcct ccgtaaatct
     9181 cgtccagacc ctctatgcaa ttaagtatta tttcagatga acccgacaag taagcctcat
     9241 tgccgccgcc ataaacatta tcaacattga aagcacaaat ttgcggattc tcgtcgccca
     9301 aaataacatt tgtaccattg ggcacatcgc ctttggtgtt gcttcctccg aagacattat
     9361 gtatggttcc gcccagaacg gttacaagcg tggtgcctat tttgttgccg gttccgtatg
     9421 atatacctcc ggatttcagg ccgacatcgg cgccgggatt ctccatgcca cccaccttat
     9481 ccttgccgtt accgccgcca aacacatagt ctatttcaaa agcgccttca atcatgactt
     9541 cggtagccgg taccggagcc gcattgccgc ctccgtaaac atattcgatt gagacatcgt
     9601 cgcaaccatc aacctcaaca taggtgggag tgttggaatt tgtgggatca taggcagcaa
     9661 ggtttccgcc tccgtaaaca ccctgcacct cataggtgcc taaagtatgc gcacctccgc
     9721 ctataggagt ggtagccaat acggttactt taactgtgcc tttcggcgtg ccgtttatgt
     9781 tgttacatcc gaatatgcgt ccggagactg gaattctatc gtcaccttcg cccgtatatg
     9841 atatgccgaa tgccgctccg tttagcaata cttcaacatt accgttcact aaggccgcag
     9901 tctcggaatc acccaatccg ccgccatagg catcgccata tatctttcct ttgtataggt
     9961 acactttggt ggtgttggaa gtaccggtat ttacacttcc cagagccgaa cctccatata
    10021 cgtcaccata tatggtaacg ccgggagtag tctgtccgct tgtgcctaca tataagtcaa
    10081 cattgccgct tactgttgta ttcttaccga agccgcctcc atgtatatag ctcttgctgc
    10141 ctgcggtgcc tattgtaccg cccgtgagca tcaggttaac attgccccct atggttccgg
    10201 tgacattaca gcctccgtac acgccatcgg aaattgttcc gtttgatatg gttatcccgg
    10261 tattgccgtt aaccccggca ttgtttccac cgccgtacac agcgccgtcc attgtgccgc
    10321 cactgagcgt caatgttgta ttacctgtca cgagtgcagc atcgcctccg ccgaatatat
    10381 cggtaaatgt tccgccgctt acagttactg tagtatttcc cgttacagtt gcagttgcgc
    10441 ctttacctcc gccgaacagc cgtgtgcccg aactgccggt aactgccgac actatgggac
    10501 tgccggtcac gggtgcagcg tcacctccgc cgaatacatc tcctcctata atgccgcccg
    10561 tgaggtttac acgcgggtta gcggttactg cggcagtctg tccaagacct ccgccataga
    10621 cattgccttc aatagttccg cttgttatat ttacctgagg gtagtttccc gagttggagt
    10681 atggggccat gttgccgcct ccatatatat tgcctgccag caaggcgcaa ctattctcag
    10741 cggcatcgtt aacattgact gttactgttc cgcctattgc cccatttgtg ttactgccgc
    10801 cgaatacatt gtctatagtg ccgccgtaaa ttttggta
//
LOCUS       DAOSGL010000038        10821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5125_length_10821_cov_4.109419, whole genome shotgun sequence.
ACCESSION   DAOSGL010000038 DAOSGL010000000
VERSION     DAOSGL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5125_length_10821_cov_4.109419"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKY68_02280"
     CDS             complement(<1..946)
                     /locus_tag="PKY68_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HOH23899.1"
                     /translation="MKTKMKQIKEKTTKGNLLLSVLIAVVAIVFAACSSDVVLNDIAE
                     SQTDTPKAIGFTSFSEKITRADTDLEYYHNTFVVYGTKQSTVDNAIQYVFGGKATAPG
                     NQDGVTCTYDAASTTVTGYWKYDNPRYWDKQAEYDFIAYAPDNAPLRYFYNAADAQVG
                     DPNNKFVTNADYTLAGTNLQATATTAEKVKGFTGDVDLDIMISTAKSQSGSNNADVAL
                     VFKHILSKLNVTFVKAAVLDGATVTVTSVEITGLKEKGSYKESDYVNDAFTKTSGWTA
                     EAVTPSTYKLAYSGSQVLNASAGSALYFIESLVMPQTIA"
     gene            complement(995..2221)
                     /locus_tag="PKY68_02285"
     CDS             complement(995..2221)
                     /locus_tag="PKY68_02285"
                     /inference="COORDINATES: protein motif:HMM:NF028454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5119 domain-containing protein"
                     /protein_id="HOH23900.1"
                     /translation="MVAAVKFYAKLRFTALIIITMIMGAVLSGCERKDLYLRVDQAEI
                     NIAIYDIQLDLLWQPSWRLELDYEWLAKYGPLGYPEKPELVKGIIYDVDTIEHKRTSY
                     FSSIFNSTGGRVSLKTRCTYNMMFYNFGTEWILFQQSEDYDYYNATTRTSSQTSWTRT
                     ASESEDSEMPEAHKSYIEVNQPDAIYGVLTDDLYVSDDPGEYEKEFNEDGRPVYIYKV
                     DCVLDPYVFIYIFQFVVKNNVDEEGQIITGAHGITVTGLSQGVEMFSRKTFDNTISIT
                     TEDIKEMQHHKNIKLQGDSAAFDGDIFVARMLTWGLPGINPMEKTLNNEPVEVIDNNY
                     VGVGLKLRNGYTYTITRDITEQMHKRPTGGIITVVIDAGKVPEEYLEHKPETGGGGFN
                     ASVEDWENEIEAELVI"
     gene            complement(2268..3236)
                     /locus_tag="PKY68_02290"
     CDS             complement(2268..3236)
                     /locus_tag="PKY68_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH23901.1"
                     /translation="MGTNITLKPGMPQHGRCDVDTLGSICRGELTFLRVPTTIKRTQP
                     ILSNTKLQSSTIPFLKPVNAKDSILTERVSPLRFRQGSAQLFRTYMDNDETLEAIDEA
                     LNILQYDDRARLSRITIESFTSPEGDPELNRDLSQRRAKTLRDEIVNLHPEISDNQFE
                     IISGVEDWQGLRDLVAQSVMPYRDEILDIIDNTDDNSVRKLKLKRLRGGVPYNSLIDL
                     YCPLLRDTCYVHIWFEEKPDMEAMIINDAIENIEQNLLDDALELLSTVEYDQRSWNAL
                     GTCYLIKGEVSRARHYFVQAVDAGNGDAKSNLLLIGTKETFDLDAN"
     gene            complement(3695..5497)
                     /gene="typA"
                     /locus_tag="PKY68_02295"
     CDS             complement(3695..5497)
                     /gene="typA"
                     /locus_tag="PKY68_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOH23902.1"
                     /translation="MQEQAIRNIAIIAHVDHGKTTLVDKMLFAGHLFRNNESAGELIM
                     DNNELERERGITILSKNVSINYKGVKINIIDTPGHADFGGEVERVLNMADGVLLLVDA
                     FEGPMPQTRFVLQKALEIGLKPIVVINKVDKPNCRPIEVQEAVFDLMFSLNATEDQLD
                     FPTVFGSAKRSWMSDDYRRETRDITYLLDRIVEHIPAPKRREGPAQMLVTSLDYSPYV
                     GRIAIGRVYRGSFRPNQDVSLIKRDGTVIKSRIKELNEFVGFGRKPSAEVSSGDICAM
                     VGIEQFDIGDTIADVNAPEALKPIAIDEPTMSMVFTINNSPFFGKDGKYITSRHIHER
                     LMRELDKNLAMRVEKDPERDIWTVFGRGVLHLSILIETMRREGYELQVGQPRVIIKEI
                     KGEKCEPIEEMTINVTEEAASKMIDVVSRRKGELTKMESKNGRVHLEFEIPSRGIIGL
                     RSLLLTLSSGEAVVAHRFKEYQPYKGEIEKRENGSMIAMETGVAVAYGMDKLQDRGRF
                     FIHPGDEVYAGQVVGESNKEGDLVVNVNKTKKLTNVRASGSDEKVNLPPPVIFSLEEA
                     LEYIKADEYVEVTPRFMRMRKIILDENERKRQNK"
     gene            complement(5648..6727)
                     /locus_tag="PKY68_02300"
     CDS             complement(5648..6727)
                     /locus_tag="PKY68_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOH23903.1"
                     /translation="MSIELKKNCKYAMLVPTSIGLRITPDNGQPVHSSDVFHLQVTSA
                     ETNVASVSSILGLPVKVLTTFVKGSPFAHYIKANLRSRNMDFEGVDVDQGGPWGYRHQ
                     INIADSGYGSRGPRVWNDRAGEVGRTLNVKDFDLDRIFGQEGVQIVHLSGLIGALSQD
                     TSKFCLELARVAKKYGTRISFDLNHRASFWKGREKELHEIFTEIASISDILIGNEEDY
                     QLCLGIKGPEAGGKDITAKIDGFKEMIKRVKAAFPNASVFANTLREVNHSNSHEWGAI
                     MLEGENWHVIEPREIHVLDRIGGGDGFVGGMLYGILKGWAPEKWAQFGWASGVLATTF
                     LTDYGQPADEEQIWSIWEGNARVKR"
     gene            complement(6871..8097)
                     /locus_tag="PKY68_02305"
     CDS             complement(6871..8097)
                     /locus_tag="PKY68_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOH23904.1"
                     /translation="MKKETFLLCALLLFACEQQVDGDKIKAREDIDLTRSQEVLVEKG
                     NTFAFRLLGQVNLEESKENFCISPFSVSQALSMTLNGAAGNTATEMISALGFEGYTQS
                     EINAYQQKMNQALLSLDPSTNIGIANSIWIREDFAVRKEFVDTNTYYFDAEVQNLPFD
                     AAALNAINNWCDVKTKGRIDKILDGIDPDDIMFLLNALYFKGIWIHKFDKTKTEEVDF
                     YLAGGEKTKVNMMYQKAEFPYYEDETVQVVELPFGNEAFSMVFVLPRQGVDMDALIET
                     LDAPCWANWLHRLRETELNLKLPRFNLEYEKELIPTMSALGMPSAFDSQMADFSLINA
                     DQDLYIGLLKQKTFVKVDEEGAEAAAVTIVGIKLTSAPLDEIHMTLDRPFLFVIKEKS
                     TSCVLFMGRIGNPQDK"
     gene            complement(8229..8984)
                     /locus_tag="PKY68_02310"
     CDS             complement(8229..8984)
                     /locus_tag="PKY68_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH23905.1"
                     /translation="MLHFLSERLSEDYRVLMAREGQEGLDMLRANDVQLIISDWMMPG
                     MDGIEFCRKVREDQFTSHIPFILLTAKTDIDSKLHGLDCGADAYIEKPFSMGYLKATV
                     RNLLELRRMLIQKFSKMPLVHLSSIAGNTVEQEFLLRMNQLIEENFANPELSVDFLAK
                     EMCISRSRLFAKIKTLIDVTPNELIQIVRLKKAAALLLEQRYMVGEVAYMVGFNNPSY
                     FSKCFQKQFGMKPFEFVTSHRNKQNTEGSGPVA"
     gene            complement(8936..>10821)
                     /locus_tag="PKY68_02315"
     CDS             complement(8936..>10821)
                     /locus_tag="PKY68_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH23906.1"
                     /translation="NRYNPAKDNFIRVRELGCLTFDIDQDKEGRIWFATESEGIFCHN
                     PADNSWKNYRRSDAPGSLGSNTVNSIYIDKNNKIYLGTGNGVYCFDAEKESFYPLDLN
                     LPSKNVCCIIGYEEELWITTSGGLVHYTSGGKSTTYTTRDGLRSEHFLPNSGLLASDG
                     KIYIGSVNGFNAFYPYKIQRNAYVPPVVITGLLVNNQALADEGISPQYTRKIKLTHKE
                     NMLSFLYASLSYSSPESNQYAFMMEGLDRDWNFVGNQTKATYTNLAPGDYVFKVKGSN
                     NNGIWNEKPSTIMLTISPPLLLSPGFKILYFALILLILTLIISNIVRISERNHQRLME
                     KLSIEKEKEVHNAKIQFFTTIAHEIRNPVSLIIAPLDRILKIPELNSGGLAEDLKMIH
                     RNSQRLLFLVNQLLDFRKIEQGGSPTIHKAKHNLGKLLDDVSLRFRPLIEQEGKRFEY
                     KFDEQNFETCFDYEALTKVLSNLLSNAVKYTKDYILLSAKVDRDKEIIEILVQDNGCG
                     IDPDEQEQVFAPFYQAKDPMPGTGIGLSIVKSMVEAHNGTIRLESEPGRGCSFYIELP
                     FCDWTKKLPRQSQLQLFFLRTLCLKAPGKIMSRMLPPFCWSKTTAKCCISSANAFLKI
                     TGC"
BASE COUNT         2801 a         2547 c         2340 g         3133 t
ORIGIN      
        1 cggcaattgt ctgaggcatt accagcgatt cgataaaata gagagctgag cccgctgagg
       61 cattaaggac ctgtgaacct gaataggcca gtttgtatgt gctgggagtt acagcttcgg
      121 cagtccagcc gcttgttttt gtgaatgcgt cgttaacata gtcgctctcc ttatagctgc
      181 ctttctcttt aagacctgtt atctcaacgc tggtgacggt cacagttgca ccgtcaagta
      241 cagccgcctt gacaaaagtg acattcagct ttgataggat gtgcttgaat accagagcca
      301 cgtcggcatt gttgcttccg ctttgcgact ttgcagtcga tatcataatg tcaagatcaa
      361 catcgccggt aaagcccttt accttttcag ctgtagtggc tgtggcttgc aggttcgtgc
      421 cggccaaagt atagtccgcg ttggtgacaa acttgttatt tggatcacca acctgggcat
      481 ctgctgcgtt gtaaaagtag cgaagcggag cattgtccgg agcataagct ataaaatcat
      541 attcagcctg tttgtcccag taacgggggt tgtcgtattt ccaatagccc gtaacagtgg
      601 tggatgctgc gtcataggtg catgttacac catcctgatt acctggtgct gtagcctttc
      661 cgccgaacac gtactggatg gcattgtcaa cggttgactg cttagtaccg tacacaacga
      721 atgtgttgtg gtaatattcc agatcggtat cggcacgcgt gatcttttcg gagaacgatg
      781 tgaacccgat agccttgggc gtatcggtct gtgactcggc aatatcattg agaacgacat
      841 cgctcgagca agctgcaaac acgattgcga caactgcaat caatacactc aaaaggaggt
      901 ttccttttgt cgtcttttct ttgatttgtt tcatttttgt tttcattttg atttgatttt
      961 aatatgagtt aataatatga ttctatcttg ttttttagat cactaattcg gcttcaattt
     1021 cattttccca atcctcaacg ctggcgttga acccgcctcc gcccgtttcg ggcttatgtt
     1081 ccagatactc ttcaggcact ttcccggcat cgattactac ggttattata cctccggtgg
     1141 ggcgcttgtg catttgttcg gttatgtcgc gtgttatagt gtatgtataa ccgttccgaa
     1201 gtttcaagcc tacgcctaca tagttgttgt cgataacctc aacaggttcg ttattcaggg
     1261 tcttttccat aggattgatg cccggcagac cccaggtaag cattctggcc acgaaaatat
     1321 cgccgtcaaa tgcggcactg tcaccctgta atttaatatt cttatgatgt tgcatctcct
     1381 ttatgtcctc agtcgttatc gaaatcgtgt tgtcgaaggt tttacgactg aacatctcaa
     1441 caccctgtga aagtcccgtt acggtaattc catgcgctcc cgtaatgatt tgtccttctt
     1501 cgtcaacatt atttttgact acgaactgga aaatgtatat gaaaacgtag gggtcgagta
     1561 cgcagtcgac tttgtatata taaaccggcc tgccatcttc gttaaattct ttttcatatt
     1621 ctccgggatc gtccgataca taaagatcgt cggtgagaac tccgtaaatt gcatcgggtt
     1681 ggttcacttc tatgtagctt ttgtgggctt ccggcatttc gctgtcctca ctctccgatg
     1741 cggttcttgt ccacgaagtc tgtgaactgg ttcgggttgt agcattataa taatcataat
     1801 cctcgctctg ttggaacaat atccattccg ttccgaagtt gtagaacatc atattatagg
     1861 tgcagcgggt tttcaaagat acacggccgc ccgttgaatt gaatatgctg gagaaataac
     1921 ttgttctctt gtgctctata gtatccacat cgtaaatgat ccctttaaca agctcaggct
     1981 tttcgggata gcccagagga ccgtatttgg ccagccattc ataatcgagc tcaagacgcc
     2041 acgacggttg ccagagcaaa tccaactgta tatcgtatat cgcaatattt atttcagctt
     2101 ggtcaacacg cagataaaga tctttacgct cacaaccgga taaaacagcc cccattatca
     2161 tcgtgatgat gataagggct gtaaaccgta gttttgcgta aaatttgact gctgcaacca
     2221 tgctcttgtt cctattttat atttttaatg tccactaatc cactgcatca attcgcatcc
     2281 agatcaaacg tttccttggt tccaatcaac agaagattcg atttggcatc gccgttgcct
     2341 gcatccacag cctgtacaaa ataatgtctg gctcttgaca cttctccctt tattaaataa
     2401 caggtcccca aagcattcca ggaacgctga tcgtattcta ctgttgataa cagttccaaa
     2461 gcatcgtcta acagattttg ttcgatattt tcgatggcat cattaatgat cattgcttcc
     2521 atatcgggct tttcttcgaa ccagatatga acgtaacaag tatctctgag caggggacaa
     2581 tacaaatcga tcagggaatt gtagggtaca cctcctctaa gacgtttcaa tttcaacttc
     2641 cttaccgagt tatcgtcggt attgtcgatg atatccagga tttcgtcgcg ataaggcatc
     2701 acactttgtg caaccagatc gcgtaaacct tgccaatctt caactccgga aataatttca
     2761 aactgattgt cggaaatttc cggatgtaaa ttgacaatct cgtcgcgcaa agtcttggcc
     2821 cttctttggg ataaatcgcg gtttaattcc ggatctcctt cgggagaagt aaagctctca
     2881 atggtaatcc ttgataaccg ggctctatcg tcgtattgca aaatattcaa agcttcgtct
     2941 atagcctcaa gggtttcatc gttatccatg taagtcctga ataactgagc gctgccctga
     3001 cggaaacgca aaggagatac tctttcggtc aggatggaat cttttgcgtt taccggcttc
     3061 aaaaaaggaa tagttgaact ctgaagtttc gtgttgctga ggataggctg ggttcttttg
     3121 atcgtggtgg gaacacgcaa aaaggtcaat tcgcctctgc aaattgatcc caatgtatcc
     3181 acatcacagc gtccgtgttg tggcatacct ggtttaagag tgatatttgt tcccatttga
     3241 tcttcgcgcg atacgtacgt acgttataaa agcaaatatc gtgccatgtt ttattaaaaa
     3301 aacagaatta tagactccac ccggggctaa actgcgactc tgtggcactt taacctctgt
     3361 ttcaaaatta aaagtggccg tgctgaaata cggcacaccg gttttaaaca ccaacaactt
     3421 accggacata caacaagtgt ttgctaatct aacatagcaa ttagccgata ttttacataa
     3481 ttcatggtct ggaacggcaa agaaaagtat ccggcaacta tctgccggtc aaacaattaa
     3541 aaaccaacaa ccttaaaata aaataatcta agcccagttt cagacagcta cgaaaataca
     3601 caataaaatt gtgtcaaacg tagtaaaaaa ttaaaaaaaa gaaggggctt ctaattttta
     3661 acatttacac ttacgaagaa cgttgtgttt ttaactattt attttgtcgt ttgcgctcgt
     3721 tttcgtccaa aatgatttta cgcatacgca taaaacgagg agtaacctct acgtattcat
     3781 cggcctttat gtattccaag gcttcttcga gcgaaaaaat tacgggagga ggcaggttga
     3841 ctttttcgtc ggaacccgaa gctcgcacat ttgttaattt tttcgtctta tttacgttaa
     3901 caaccaagtc gccttctttg ttgctttcgc ccaccacctg gccggcataa acttcgtcgc
     3961 cgggatgaat aaaaaaacga ccccggtctt gcaacttatc cataccgtaa gccacggcaa
     4021 cacctgtttc catggcaatc atagagccgt tttctctttt ttcgatttcg cccttatagg
     4081 gttgatattc tttgaaacga tgagcaacga cagcttcgcc cgacgataaa gtgagcaaga
     4141 ggctgcgtaa acctataatc cctctggagg gaatctcaaa ttccagatgt acgcgtccgt
     4201 ttttgctttc cattttggtt aactcaccct tacggcggct gactacatcg atcattttac
     4261 tggcggcttc ttccgtgaca ttgatggtca tttcttcgat gggctcgcat ttctctccct
     4321 ttatttcctt gatgatgact ctgggttgtc ctacctgaag ttcataccct tcgcgccgca
     4381 tggtttctat cagtatcgaa agatgcaaaa cgcccctgcc gaacaccgtc cagatatccc
     4441 gctcgggatc tttttccact ctcatagcca ggtttttgtc taattcccgc atcagacgtt
     4501 cgtgtatatg cctcgaggtg atgtatttgc cgtcttttcc gaaaaagggt gaattattga
     4561 tggtaaacac catgctcatg gtgggttcgt ctatggcaat gggcttcaga gcttcgggag
     4621 cgttcacatc ggctatggta tcgccaatat caaactgctc tatgccaacc atagcgcaaa
     4681 tatcgcccga actcacctcg gcagacggtt tacgcccaaa accgacaaat tcattcagct
     4741 ccttgatgcg gctttttatc accgtcccgt ctcgctttat caggctgacg tcctgattgg
     4801 gacgaaagct tccccggtac acgcgaccta tggcaatacg tccgacatag ggagaataat
     4861 ccaacgaagt aaccagcatc tgggcggggc cttcgcggcg tttgggcgcc ggaatatgtt
     4921 ccactattcg gtcgagcaaa taggtgatat cccgtgtctc gcgacggtaa tcgtccgaca
     4981 tccaggaacg tttggccgaa ccgaaaacgg tgggaaaatc caattgatct tcggtggcat
     5041 tgaggctgaa catcaaatcg aaaacagctt cctgcacttc tataggccgg caattgggtt
     5101 tgtccacttt gttgattacc actatgggct ttaatcctat ttcgagggct ttttgcaaga
     5161 cgaaacgggt ctgaggcatc ggaccttcaa aagcatccac cagcagcaat acaccatcgg
     5221 ccatattgag tacccgctcc acctcgccgc cgaaatcggc gtgtccgggt gtatcgatga
     5281 tgttgatctt tacacccttg tagttgatgg aaacgttttt ggacaatata gtgatgcctc
     5341 tttcgcgttc cagctcgttg ttgtccataa tcaattcgcc ggcactttcg ttgttgcgaa
     5401 acaaatgacc ggcaaacaac atcttgtcga ccagagtggt tttaccgtgg tcgacatggg
     5461 caattatggc aatgttcctg atggcctgct cttgcataaa tgtggaaaaa ttaaatcagc
     5521 gcgcgaaagt agtggttttt ttgcatatat ctgcaaaaaa aagctgtccg ctcattgagc
     5581 gaacagcccg tagtgtgata aaaaagtgtc ggaaagtata ttcgactgaa ctttccggct
     5641 tgtttattta acgctttaca cgtgcattgc cttcccagat actccaaatc tgctcttcgt
     5701 cggcaggttg tccgtaatcg gtaaggaaag tggtggctaa aacaccgctg gcccagccaa
     5761 actgtgccca tttttcgggg gcccagcctt tcagaatacc gtagagcata ccccctacaa
     5821 aaccgtcgcc tccgccgatg cggtccaata catgtatttc gcggggctca atcacatgcc
     5881 agttttcgcc ttcgagcata atggcacccc attcgtggct gttgctgtgg tttacttcgc
     5941 gcagggtgtt ggcaaaaacc gaagcattgg ggaaggcggc cttgacgcgc ttgatcattt
     6001 ccttgaatcc gtcaattttg gcggtaatat ctttgcctcc ggcttcgggg cctttgatgc
     6061 ccagacaaag ttggtaatct tcttcgttgc cgatcagaat atccgatatg gaagcgatct
     6121 cggtaaatat ctcgtgcaat tccttttcgc ggcctttcca gaacgaagcc cggtggttca
     6181 aatcgaaaga aatacgggtg ccgtatttct tggccacgcg ggcaagttcc aggcagaatt
     6241 tgctggtatc ctgcgacaaa gcgcctatca gacccgagag atgtacaatt tgtacgcctt
     6301 cctgtccaaa aatcctgtcg agatcaaaat ctttcacgtt cagtgtgcgc cccacttcgc
     6361 cggcacggtc gttccagacc ctgggtcccc gcgaaccgta accgctatcg gctatattaa
     6421 tctgatgacg atagccccag gggcctccct gatctacatc cactccttcg aaatccatgt
     6481 tgcggctgcg caggttggct ttgatgtagt gagcgaaggg actgcctttt acaaaagtgg
     6541 tcagcacttt taccggcaga ccaaggatag aagaaacact ggccacattc gtttcggcac
     6601 tggtaacctg cagatgaaaa acatcactgc tatggacggg ttgcccgttg tcgggcgtga
     6661 tacgcaaacc tatgctggta ggaacaagca tggcatactt gcaatttttt ttaagttcga
     6721 tactcataat ttactggtta tttaaaatat aatggaatgt ttgtttcttc cgaccgaatg
     6781 agctacaaat ataaaaaaag cggaggaatt ttagcagtaa atcctccgct tttaattatc
     6841 ggaaaagctc aagggcttaa aatcaacgcc ttatttatcc tgaggattcc cgattctacc
     6901 cataaacaga acacaagaag tgcttttttc ttttataacg aacaggaaag gcctgtctag
     6961 tgtcatatgt atctcatcca gtggggcaga ggtcagtttt atccccacta tcgttacggc
     7021 agcggcttcg gctccttcct cgtctacttt gacaaaggtt ttttgcttca acaagccaat
     7081 gtacaaatcc tgatctgcat tgatcagact aaaatcggcc atttgggaat cgaaagccga
     7141 tggcatgccc aaagcagaca tggtgggtat cagttctttt tcgtattcca gattgaaacg
     7201 gggcaattta agattaagtt ccgtttccct gagacgatgc agccagttag cccaacaagg
     7261 agcgtcgagg gtttcaatca atgcatccat atcaacaccc tggcggggca aaacaaatac
     7321 catgctgaaa gcctcgttcc cgaagggcag ttctacaacc tgaacggtct cgtcttcgta
     7381 ataaggaaat tccgcttttt gatacatcat attcaccttg gtcttttcgc cgccggccaa
     7441 ataaaaatcg acttcttcgg ttttggtttt atcgaattta tgtatccaaa tgcctttgaa
     7501 ataaagggcg ttaagcaaaa acataatgtc gtccggatca ataccgtcga ggattttatc
     7561 gattcgaccc ttggttttta catcgcacca attgtttatg gcatttaaag cagctgcatc
     7621 aaagggaaga ttttgcactt cggcatcgaa gtaataggta ttggtatcga caaactcctt
     7681 gcgcacggca aaatcttcgc gaatccaaat ggaattggca atacctatgt tggtcgaagg
     7741 atccaggctc aacagggcct gattcatttt ttgctgatag gcgttgattt cgctttgagt
     7801 atagccttcg aagcccaggg cagaaatcat ttcggtagcc gtattgccgg ctgctccgtt
     7861 taaagtcatc gacaaagcct gcgaaacgct gaaaggcgaa atgcaaaagt tctctttcga
     7921 ctcttccaga ttgacctgcc ccaacaaacg gaaggcaaaa gtgttgcctt tttccaccaa
     7981 cacttcctga ctacgggtta aatcaatgtc ttctctggct ttgattttat cgccgtcaac
     8041 ctgttgttca caagcaaaca acaaaagtgc acacaataag aaagtttcct ttttcatggc
     8101 ttataatcta attaaatagg aatactgagt aatcacttaa tttaggaatg aaattactca
     8161 aactaagacc gaaatccgga aaaaacgcta cataattgac ggcatcctca caagaatcga
     8221 cggcatcctc acgcaacagg gccggaacct tcggtattct gtttattgcg gtggctggtt
     8281 acaaattcaa aaggcttcat tccaaattgt ttctgaaagc acttggaaaa atacgaggga
     8341 ttgttgaaac ctaccatata ggctacttcg ccaaccatgt agcgttgttc caacaaaaga
     8401 gccgccgctt ttttgaggcg cactatctga atcaattcgt tgggtgtgac atcgatcagg
     8461 gtttttattt tggcaaacaa acgggaacgg ctgatgcaca tttctttagc caaaaagtcc
     8521 accgaaagct cgggattggc aaaattttcc tctatcagct gattcatcct caataaaaat
     8581 tcctgttcca cagtattacc ggcaatgctg ctgaggtgca ccaggggcat tttagaaaat
     8641 ttctgaatca acatacgccg taattcgagc aagtttctta cggtggcttt caaataaccc
     8701 atagagaagg gtttctcaat ataagcatcc gcaccgcaat ccaggccatg caatttggaa
     8761 tcgatatcgg ttttggcggt cagtaaaata aaaggaatat ggctggtaaa ctgatcttcg
     8821 cgcactttgc gacaaaattc aatgccatcc atacccggca tcatccagtc gctgatgatc
     8881 aattgaacat cgttggccct cagcatatcc agaccctcct gcccttccct ggccatcagc
     8941 accctgtaat cttcagaaag gcgttcgctg aggaaatgca gcatttcgcg gttgtcttcg
     9001 accaacaaaa tggtggcaac atccgagaca tgatcttccc cggagctttc agacacaatg
     9061 tcctgaggaa gaacaactga agttgcgact gcctcgggag ctttttcgtc caatcacaaa
     9121 aaggcagttc aatgtaaaaa ctgcaacccc ggccgggctc agattccaga cgtatggttc
     9181 cgttgtgagc ctccaccatg cttttaacta tactcagccc tatacctgta ccgggcatgg
     9241 ggtctttggc ctgatagaaa ggtgcgaata cctgttcctg ctcatcggga tctataccgc
     9301 agccgttgtc ctgaacgaga atttcaatta tttctttgtc tctgtccacc ttagcgctga
     9361 gcagtatgta atctttggtg tatttaacgg cattcgataa taaattactc aacaccttgg
     9421 tcaatgcttc gtaatcgaag catgtttcaa aattctgctc atcaaatttg tattcaaagc
     9481 gtttgccttc ttgctcaatc aaaggacgga aacgcaggct gacatcgtcg agcaattttc
     9541 cgaggttgtg ttttgctttg tgaatagtcg ggctgcctcc ctgctctatt ttccggaaat
     9601 ccagcaattg attgaccaaa aagagcaaac gctgactgtt gcgatgaatc attttgaggt
     9661 cttcggccag tcctccggag ttcaattcgg gaatttttag aattctgtcg agcggggcga
     9721 taatcaagga taccggattg cggatttcat gagctatagt agtaaaaaat tggattttgg
     9781 cgttgtgtac ctccttttct ttttcaatac tgagtttttc cattagtcgc tgatgattgc
     9841 gttcgctaat tcgaacaata ttgctgataa tcagcgtcag aatcaacagt atcagggcaa
     9901 agtagagaat cttgaatcca gggctaagaa gcaagggagg ggaaatagtc agcataatcg
     9961 tgcttggctt ttcgttccag attccgttgt tgttggagcc ttttaccttg aacacataat
    10021 cacccggagc taaattggta taggtggctt tggtctgatt gcctacaaaa ttccagtccc
    10081 ggtccaaacc ctccatcata aaggcatatt gattgctttc gggactactg taactaaggg
    10141 aggcatacaa aaagctcaac atattctcct tatgtgtaag ctttatttta cgtgtatact
    10201 gcggagaaat tccctcatcg gccagagctt gattattaac caacagaccg gtaattacca
    10261 ccggaggcac ataagcgttt ctctgaattt tgtagggata aaaagcattg aagccgttga
    10321 ccgatccgat gtagatttta ccatccgagg ccaataagcc cgaattcggt aaaaaatgtt
    10381 cgctacgtag tccgtccctg gtggtatatg tagtgctttt ccctccggaa gtgtaatgta
    10441 ccagccctcc ggaagtggtt atccacaatt cctcttcgta gcctatgatg caacacacat
    10501 ttttgctggg taaattcaga tccagaggat aaaaactttc tttttcggca tcgaaacaat
    10561 aaacgccgtt tccggtgcca agataaattt tattgttttt gtctatgtag atactattga
    10621 cggtgttgct gcccaaggag cccggagcgt cgcttctgcg gtaatttttc caactgttgt
    10681 ccgcggggtt gtggcaaaat attccttcgc tctcggtagc aaaccaaatc cgcccttcct
    10741 tatcctgatc gatgtcgaag gtgaggcagc ccaattccct tacccggata aaattgtcct
    10801 ttgcggggtt ataacggttt a
//
LOCUS       DAOSGL010000039        10741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5179_length_10741_cov_4.252761, whole genome shotgun sequence.
ACCESSION   DAOSGL010000039 DAOSGL010000000
VERSION     DAOSGL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5179_length_10741_cov_4.252761"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1631)
                     /locus_tag="PKY68_02320"
     CDS             complement(15..1631)
                     /locus_tag="PKY68_02320"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH23907.1"
                     /translation="MKYITKLVLLLSLGALLTTSCEKMLDTDSERFVPEKDHIIDDEL
                     DAMYAMMGVLTKLQLLGDRYVVLGELRADLMDVTENSESELRALSNLEAGKDNPWLEA
                     DDYYKVINHCNYLINNLDTSLMSGGKNILLPEFAQAKVIRAWTYMQLALNYGKAYYLS
                     TPILTIEEALDSYPVLEKEAILDSLILDLESIQYVQNPDYGDFGSSFYSTYYFLSARF
                     LLADLYLWKNEYAKAASKYFELALDEGRSVTSTYTSTWRSVTPPLVSPNWHAIFDKLR
                     FGPEITAAIGYYNSTSFGDYKESSLALMTQTNYTLAPSANALELFENQVYMYGEEEVA
                     TIIVPGDLRGESGSYEYTERLEDNTIQISDKPRITIYNGEAVYLQRNALLYLRYAEAV
                     NQLNKPSLALAAINYGLSKANIQTYVKPNEIDSGVPWQEFSADVFTSNVGTRSRGQGN
                     KSKVTFPGSLQTLQDSVAFVEEVLVEECALESAFQGNRFHDLMRMSLHNNNYSIIAGA
                     IAKKHKTNYTAVYAKMMDPANWYLPYPDAE"
     gene            complement(1653..4862)
                     /locus_tag="PKY68_02325"
     CDS             complement(1653..4862)
                     /locus_tag="PKY68_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH23908.1"
                     /translation="MKNDFKSRVCRLALAVILLLGSTGLAAQQSEIKPVKSQVFIEGK
                     ITDDATGEPINAATIKMGKSSAFTNEEGLFKLQVIGELKPTIIVTAPGYDTREVPHRG
                     QAVMNFTLSSDQFGKALGSKPKKAELDEFINKEGAAVVTMEEIDLTNPMESVESVLQS
                     KFGTIRSVTHSGMPGVGAAMFLRGINTLNVNAKPLFILDGVMLQNFDDQASVHDGFFS
                     NPLTHIDLREVESITVIKDAVSLYGAKAANGVVMIETRRSKDMVTHITANINGGVRSK
                     PQTIPVMDAEQHRLLVSDILKDHPDYDAARLDKMKFFNDNPTFLYYKQNHNNTAWKDF
                     VYRDAVSQSYSLAATGGDEVGMYAIFVNYRNNQGLVRNTSMQQISTRFNSDIVLRDNI
                     SISTKFALSNQSYDLKNDGIISPTSPSGLSYIKAPALYPYSYAKMTGIISTLFEDYDE
                     LGVNNPEAILKKSQQINTQNSFIASAYPTWEINKNLVWKATLGYSMNKLFESYFTPEE
                     GVAPYYLTPSISDYVMKDIIVENEARSQTMRSIQTNLDNRLVFTKQFKKHHLQAIGGF
                     RYIGHRMGMDYIKGFNTGEDNLVNISKDLKYRVTDGIDQRTKELSWYLSGSWNYNEKY
                     FAGLAASMDASSRFGYNLKGNALDLFGVSWGLFPSVNASWIASSEDFLAPLKGLDLLK
                     FRLSYGLTGNDDIEDYVTYPYFSSENYLGGGVALVLANLGNPQLKWETTTKANAGVDL
                     SLFNNRFSLSADLFSSNTSDLLVLKQLPSIAGFDYYWSNDGELSNKGYELSANARLLN
                     IGKFRWEVGASLAHYQNELLRLADGDYSTTAYGAEILTAVGQPVGVFYGYKTEGVYGT
                     QAEAEADGLVNRDEVTHLIDYSFGAGDVIFEDFVADGVIDENDKQIIGNPNPDFVGTL
                     SSRIGYKGVNLDILFNYSYGNDVYNFIRRQLESVSDVHNQSTAVLNRWRSENDVTNMP
                     KAVWGDPAGNARFSDRWIEDASYLRLKTLCLSWELPYRIPMVQNLTVWASANNLWTGT
                     KYLGADPETSISSRALYQGIDIGLTPQSPSFYIGVKIGL"
     gene            complement(4885..6474)
                     /locus_tag="PKY68_02330"
     CDS             complement(4885..6474)
                     /locus_tag="PKY68_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH23909.1"
                     /translation="MKHLTKTVLFTVLALILYSCHQSWEDHYKTQNEALLEKKLMELI
                     NEQSDLSLFASMLKKSGYDKVLSANQAYTVWAPDNAALASVDTSDMDDVNKIVRNHIA
                     RFSYPSSGVQNVAVSMLNGKNVHFIQKEEGLVFGDRKLLKEDLAVGNGILHVIDGMVT
                     YYPNVWEYLSSAANVDSLRDFMYSFEEYFFDERNSLIIGTNDQGQILYDSIIIYSNEL
                     FDYLGNLNHEDSLYTMLFPDNQAWTEAYDTISTYFKYSGSVSTDTVLKAEADLNQSSI
                     TKFVIAKDLIFRGNMTRDELLASDTLVSTGGHVFRDPALLFASATDSTMSNGRCFITP
                     KLNYSTDVWCKPIVVEAEHVRGRIHDRCQLNNRNSLRSWLDTAISNSGYIEVSPISAS
                     AQPIVTFDIPNTLSTAYNIYCVFVPPNVNNKNKDTAELLPTKIRYTIEYLSASGNSTS
                     RTYSRPALYVTNPREMTKMLVTQTAAGVPDPFRFNFCNYEEELTTVRITLTNIVTNAE
                     FESKKFTRDMLIDCIILEPVY"
     gene            complement(6509..8098)
                     /locus_tag="PKY68_02335"
     CDS             complement(6509..8098)
                     /locus_tag="PKY68_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH23910.1"
                     /translation="MKTAFHKIKVAFLAVLLSLGMNSCQDWLTIFPEGELILEDYWKE
                     GAHVEQVVASCYRSLIEPACIERMLVWGEVRSDNMVAGPNVGGDLRDVLDVNILTTNG
                     YANWSSFYTVINFCNTVLLYAPGVVDIDNTYSINQLNAHRAEALAIRALCYFYLVRAF
                     RDVPYITHPSVDGAEDFAMAKTEGHVILDSLILDLKEAKTYAQNNYGVSNVKHNKGRF
                     TRQSIRALMADIYLWQGRYQECVDICREFHRENALLPLASQLRLIDADFMYTEVFNRG
                     NSSESILELQFADDGITNSALNKLYGGGSSGASNSDLSAPSKSLDYSYGSMSAPFKIT
                     DVRWLSFVRGVGSGGYLFINKYVGTQPEQYDNIISYSIRGTTSNFILYRLSDIYLMEA
                     EALNELGEAHLKQALSLVNKTYMRANPSLTDSLSIVDYGSVEAMRELVLLERQREFMF
                     EGKRWFDLVRMVNKGEPIFKLTTIVRRKFNMEAGAEILAVSKMSKMDALYFPILQDEI
                     LSNELLEQNPFYQLEETSAKN"
     gene            complement(8117..>10741)
                     /locus_tag="PKY68_02340"
     CDS             complement(8117..>10741)
                     /locus_tag="PKY68_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH23911.1"
                     /translation="QYAQLIDVNPDDIESIVILKDAASTAIWGSQGANGVLQITTKRG
                     QRGPTRVSYSYRLSGSRQPKGLKMLNGDDYTMLMKQSYYNPTLENLSAGIPELNYDTN
                     FTEYENFNNNTDWIDAVSRYGFTHDNNLTISGGGEKITFRVSAGYYSQTGTVIGQLLE
                     RFSTRSSIEYRVSDRILFTSDFAYTYSNNDRNYENLLGNAYKKMPNVSIYEQDLYGND
                     TDVYYNIRTDSQLHDSQKNLSNPVAVGMLAKNNTKSYRISPNFSLRYDLLDPYKYNST
                     LRFNSTVSFSLGNSQKSTFLPSDISNARWNSDKVNLTSNSEDESLDVQFSTSVDYRPA
                     FENRDHSLMLRANFRVNTGTSGSLGISAYGLPSTIIRDATVPGILGSTSSSTSESRGL
                     SYSWSGHYAYKGRYIFDFTANFNGSSRFGDNFKFGLFPGISGKWIISDEPFWDDYRHI
                     VNMFAFRPSWGIGGNQPSTEYLHLSRYKSYDKGYIDMIAVYPFNIRLTDLRWEKTNSI
                     NLGADLEFLKGKYAIDLNYYMKHTTDLLFNKIGLPSHTGYAQLSARNGGTMNNSGWEA
                     NLHVRDLVKAGNFRMSFNFNTSNNRNIIVELDDDILATMNPDYTYSNGEYLTRIQERN
                     PYGSIYGFRYKGVYQYNDYIEGVQESAPVVRDKEGNVIFDADGKTKPMYYAYGGSAQY
                     EFKAGDAIYEDINHDGTIDELDIVYLGNSLPKFMGGFGSTLRWKQLSLNLFFNFRTGN
                     KVVNAARMEAENMYTNNNQSIAVNWRWRKEGDVTEMPRALYNYGYNWLGSDRYVEDAS
                     FLRFKYMTFNYAVPTQYLKPYKLNTLSFYLTFQNLLVFTNYTGVDPEVGYGSRSVAKD
                     SSKTPRPKDFTLGIKIGF"
BASE COUNT         2940 a         2442 c         2366 g         2993 t
ORIGIN      
        1 tcaggatacg aaatttattc tgcatccggg tagggcaaat accaattggc cggatccatc
       61 attttggcat aaacggcagt gtaattggtt ttatgctttt tggcaatggc tccggcaatg
      121 atggaatagt tgttgttatg cagtgacatc ctcatcaaat cgtggaagcg gttgccctga
      181 aaggccgatt ccaaagcgca ttcttcgaca agcacttctt cgacaaaagc gaccgaatcc
      241 tgaagggttt gcaaactgcc cgggaaagtc actttcgact tattaccctg ccccctgctg
      301 cgggttccga cattcgaagt aaaaacatcc gcactgaatt cctgccaggg cacgccggaa
      361 tctatctcgt taggctttac gtaggtttgt atattggctt tgctcaaacc ataattaatc
      421 gctgccaaag ccagactggg tttattcaac tgatttactg cttcggcata acgtaaatac
      481 aataaagcat tacgctgcag ataaacagct tctccgttgt atatggtaat tctgggctta
      541 tcggaaatct gaatggtatt atcctctaag cgttcggtgt attcgtaaga tccgctttcg
      601 cctctcaggt caccgggaac aataatggtg gctacttctt cctcgccgta catatatacc
      661 tggttttcga atagttccaa agcattggcc gaaggagcaa gggtataatt ggtttgtgtc
      721 atcaaagcca gagacgattc tttgtaatct ccgaacgaag tggaattata ataaccgatg
      781 gcagcagtaa tctccggccc aaaacgtaat ttatcaaaaa tagcatgcca attgggcgac
      841 accaggggtg gcgtaacgga acgccaagtt gatgtatacg tggaggttac acttctgcct
      901 tcgtccaggg ctaattcaaa atattttgaa gcagcctttg cgtattcatt tttccataag
      961 tagagatctg ccaacaggaa tctggccgaa aggaaataat aggtactgta gaagctgctc
     1021 ccaaaatcac cgtaatccgg gttttgtaca tattgaatgc tttccagatc aagaataaga
     1081 gaatccagaa tagcttcctt ttccagaacg ggataagaat ccagggcttc ttcaatggta
     1141 agaataggcg tacttaagta ataagctttg ccgtaattca gggccagttg catataggtc
     1201 caggcccgaa tcactttagc ctgggcaaat tcaggtaata atatattttt ccctcccgac
     1261 atcaaagaag tatccaaatt gttaatcaaa taattgcaat gattgatcac cttgtaataa
     1321 tcgtcggctt cgagccaggg attgtcttta ccggcttcca gattgctcaa agccctgagt
     1381 tcagactctg aattttcggt cacatccata agatctgccc tgagttcacc caaaacgaca
     1441 taacgatcgc ctaataattg tagtttagtg agcactccca tcatggcata catggcatcc
     1501 agttcgtcgt cgataatatg gtccttttcg ggaacaaaac gttctgagtc cgtatccaac
     1561 attttttcgc aggaagtagt cagcaaggcg cctaaactca gtaataaaac caattttgtt
     1621 atgtatttca ttgtactgtc ttattagtat gattacaaac ctatttttac ccctatatag
     1681 aaggagggac tctgaggagt taaacctatg tctatcccct gataaagggc tcgactggaa
     1741 atcgaagttt ccggatcggc ccccaggtat ttggtgccgg tccataaatt attggccgaa
     1801 gcccataccg taagattttg aaccatcgga atacggtaag gcaactccca cgacaaacac
     1861 agtgtcttta acctcagata tgaggcatct tcgatccaac gatccgaaaa acgggcattt
     1921 cctgccggat cgccccaaac agccttaggc atattggtga cgtcgttttc cgaacgccaa
     1981 cgattcaata cggcagtgga ttgattgtgg acatcgctca ccgattcgag ttgcctgcgg
     2041 atgaaattgt acacatcgtt accgtaggaa tagttgaaca agatatcgag attgactcct
     2101 ttgtagccta tacggctgct caaagtaccc acaaaatcgg ggttggggtt tcctataatc
     2161 tgcttgtcgt tttcatcaat aactccatcg gcaacaaaat cttcaaagat aacatcaccg
     2221 gcgccaaaac tataatcaat caaatgagtc acttcgtctc tgttgaccaa accatcggct
     2281 tcggcctctg cctgagtgcc gtagacgcct tcggttttgt agccgtagaa aactccgaca
     2341 ggttggccta cggctgttaa tatttcagcg ccgtaagccg tggtggaata gtctccgtcg
     2401 gccagcctaa gcaattcatt ctgataatgt gccagcgaag cccctacttc ccagcggaat
     2461 ttaccgatat tcagcaaacg ggcgttggct gagagttcat aacctttgtt ggacagttcg
     2521 ccgtcgttgc tccagtaata atcgaaaccg gctatggaag gcaattgttt cagtaccaac
     2581 aaatcggaag tattcgagct aaacagatcg gcagacaaag aaaagcggtt gttaaataag
     2641 gaaaggtcca cgccggcatt agctttggtt gttgtttccc atttaagttg aggattgccg
     2701 aggttggcca aaaccagggc tactccgcca cccaagtaat tctcagacga gaaataagga
     2761 taggtgacat agtcctcaat atcgtcgtta cccgtcaaac cgtaactcaa cctgaatttg
     2821 agtaaatcca gacctttaag aggagcaagg aaatcttccg aagatgcgat ccaggaagca
     2881 tttaccgagg ggaaaaggcc ccagctgaca ccaaacaaat cgagggcatt acccttcagg
     2941 ttgtagccaa agcgcgagga agcatccata gaggcagcca gaccggcaaa atatttttca
     3001 ttgtaattcc aacttccgct caaataccag gacaactctt tagtccgttg atctatacca
     3061 tcggttaccc gatacttcag gtccttactg atatttacca ggttgtcttc tccggtatta
     3121 aaacctttga tataatccat tcccatcctg tggcctatat aacgaaagcc tcctatagcc
     3181 tgcaagtgat gctttttaaa ttgtttggta aaaacaaggc ggttgtcgag attggtttga
     3241 atgctgcgca tagtctggct tcgggcctca ttctctacga tgatatcctt catcacataa
     3301 tcggagatac tgggagtcag gtaatagggc gctacgcctt cttcgggtgt aaaataagat
     3361 tcaaacaatt tattcatact gtatcccaaa gtagccttcc aaaccagatt cttattaatt
     3421 tcccatgtcg gataagccga tgcgataaaa ctgttttgag tattgatttg ctgcgatttc
     3481 ttcagtatgg cctcgggatt attcacgccc aattcgtcgt aatcctcgaa caaagtggaa
     3541 ataattcccg tcatttttgc gtaggaatag ggatacaagg cgggagcctt gatgtaactc
     3601 agtcccgaag gagatgtcgg agatattatt ccatcgttct tcagatcgta actttggttg
     3661 ctcagggcaa atttggtaga aatgcttatg ttgtctctca atacgatatc cgaattaaag
     3721 cgggtactga tttgctgcat actggtatta cgcaccaatc cctggttgtt tctgtagttc
     3781 acaaaaatgg cgtacattcc cacttcgtca cccccggtgg cagccagaga ataactctga
     3841 cttacggcgt cgcggtaaac aaaatccttc caggcggtgt tattgtgatt ctgtttgtag
     3901 taaaggaaag tgggattatc gttgaaaaac ttcattttgt ccaatcttgc cgcgtcgtag
     3961 tcgggatgat cctttaaaat atcgctgacc aaaagacgat gctgctcggc atccataaca
     4021 ggaatcgttt gcggtttgga gcgcactccc ccatttatat tggctgtgat atgagttacc
     4081 atatctttgc ttcgcctggt ttctatcatc actacgccgt tggcagcctt ggccccgtac
     4141 agcgagacgg catctttaat tacggtaatg ctttctactt ctctaagatc tatatgtgtt
     4201 aagggattgg agaaaaaacc atcgtgtacc gaggcctgat cgtcgaaatt ttgcaacatt
     4261 accccgtcca agataaacaa gggtttagca ttcacattca gcgtattgat tcctctgaga
     4321 aacatagcgg cacctacgcc gggcatgcct gaatgtgtaa ccgaacgtat ggttccaaat
     4381 ttcgattgca gaacactttc gacggattcc atggggttgg tcaggtcaat ttcttccatg
     4441 gtaacaaccg ccgctccttc tttattgatg aactcgtcca attcagcctt tttaggttta
     4501 cttcccaggg cttttccaaa ttgatcgctc gacaaagtga agttcataac agcctgacct
     4561 ctgtgaggaa cctcacgggt atcgtaaccc ggagcagtaa caataatagt gggtttaagt
     4621 tctcctatca cctgaagttt aaaaaggcct tcctcgttgg taaatgcgga agattttccc
     4681 attttgatcg tagccgcatt tataggctca ccggtagcat cgtctgtgat cttgccctct
     4741 ataaaaactt gcgacttgac cggtttaatt tcgctttgtt gcgcagcaag acccgtactt
     4801 cccaataaaa gaattaccgc caaggctaaa cgacaaacac gagatttaaa atcatttttc
     4861 atagtgtaaa gcttctttta tagattagta aacgggttca agtataatac agtctatcag
     4921 catatcgcgg gtgaacttct tggattcaaa ttccgcattg gtaacaatgt tggtcagagt
     4981 aatccgcacc gtagtaagtt cttcctcgta attacaaaag ttgaagcgaa aaggatcggg
     5041 taccccggcg gcggtctgcg tgacaagcat cttggtcatt tcacgtggat tggtcacgta
     5101 cagtgcggga cgactataag ttctggaagt ggaattaccg ctggcactca aatactcaat
     5161 ggtataacgt attttggtcg gcaataattc tgccgtatcc ttattcttgt tgttcacgtt
     5221 gggcggcaca aacacacaat aaatattgta tgcggtacta agggtgttgg gaatatcaaa
     5281 agtaacgatc ggctgagcac tggcagagat ggggcttact tcaatatatc ccgaattgga
     5341 tatggcggta tcgagccatg aacgcaaaga gttacggtta tttaattggc aacgatcgtg
     5401 tatacgtccg cgaacatgtt cggcctccac cactatgggt ttacaccaga catcggtgga
     5461 ataattcagt tttggagtga tgaaacatct tccgttgctc atagtggaat cggtcgcaga
     5521 agcaaaaagt aaggcgggat ccctgaaaac atgtccgccg gtagaaacca gagtgtcact
     5581 tgccaatagt tcatctcttg tcatatttcc cctaaaaatc aaatcttttg ctataacgaa
     5641 tttggttatt gagctttgat ttaaatcggc ctcagccttt aaaacagtgt ctgttgaaac
     5701 agaaccggaa tacttgaaat aggtactgat cgtatcgtaa gcttcggtcc aggcctgatt
     5761 gtcgggaaag agcatggtgt acaggctgtc ttcgtgattc agatttccca gataatcgaa
     5821 tagttcattg gaataaataa tgatagagtc gtaaagaatc tgtccctgat cgtttgttcc
     5881 gataattaaa ctgtttcttt cgtcaaaaaa gtattcttcg aaagaataca taaaatcccg
     5941 cagggaatcg acattggcgg cgctactcag gtattcccaa acattaggat aataggtcac
     6001 cattccgtcg ataacgtgca aaataccgtt gcccaccgcg agatcctctt tgagtagttt
     6061 cctgtcgcca aaaacaagcc cctcctcttt ttgtataaaa tgaacgttct ttccgttcaa
     6121 catactgact gccacatttt gcactccgga actggggtag gaaaaacgag caatatgatt
     6181 gcgaacaatt ttgttgacat cgtccatatc gctggtatca acgcttgcca aagcggcatt
     6241 atcgggggcc cataccgtat acgcctgatt ggccgacaac actttgtcgt agcctgattt
     6301 tttgagcata gaggcaaaaa ggctcaaatc ggactgttca ttaatgagct ccatcaattt
     6361 cttttctaat aaagcctcgt tttgcgtctt gtaatgatct tcccaggact gatggcaaga
     6421 ataaagtatc aaggccaaga ctgtaaacaa aacggtttta gttaaatgct tcatattaag
     6481 tatatttatt aatcgtattg gttaagcatt aattcttggc agaggtctct tccaattgat
     6541 agaaagggtt ttgctccaat aattcgttac ttaaaatttc atcctgcaaa atcggaaaat
     6601 acaaggcatc cattttggac atcttactta cggccaaaat ttcagctcct gcttccatat
     6661 tgaatttacg gcgtactatg gtggtcagtt tgaaaatcgg ttctcctttg ttgaccattc
     6721 tgaccaagtc aaaccaacgc tttccctcga acatgaattc ccgttgacgt tccagcaaca
     6781 ccagttcgcg catggcttcc acactgccgt aatcgactat actcagcgaa tcggtcagag
     6841 agggattggc gcgcatatag gttttgttaa ccagcgaaag ggcctgtttc aaatgggctt
     6901 cacccaactc attcagcgcc tcggcctcca taagataaat atccgataag cgatagagta
     6961 taaaattact ggtagtgccc ctaatagaat aagaaataat gttgtcgtat tgttcgggtt
     7021 gagttcccac gtacttatta atgaagagat agccgcctga acccacaccc cggacaaaac
     7081 tcagccatcg tacatctgtt atcttaaagg gagccgacat actgccgtag gaataatcca
     7141 aagattttga gggagccgac agatcgctgt tagaggcacc acttgaacct cctccgtata
     7201 atttattcaa agcggagttt gttattccgt cgtccgcaaa ttgcaattcc aatatacttt
     7261 cggaggaatt tccccgattg aagacctcgg tatacatgaa atcggcatct atcaacctca
     7321 actggctggc aaggggtaag agggcattct cgcggtgaaa ttcccggcaa atatccacac
     7381 attcctgata acgtccctgc cacagataga tatcggccat cagggccctg atggattgcc
     7441 tggtaaaacg ccccttattg tgtttgacgt tgctcacacc ataattgttt tgagcatagg
     7501 ttttggcttc tttcaaatcg agaatcagcg aatcgagaat aacatgcccc tcggtcttgg
     7561 ccatggcaaa gtcttcggcg ccgtcaacgc tgggatgcgt aatatagggc acatccctga
     7621 aagccctaac cagataaaag tagcacaggg cacgtatggc cagcgcttcc gctctgtgag
     7681 cattcaactg gttaatagaa tatgtgttgt caatatcaac cactccggga gcatatagta
     7741 acaccgtatt gcaaaaatta atgaccgtat aaaagctgga ccaattagca taaccgttgg
     7801 tggtcagaat attgacatcc aaaacatctc tcaggtcacc cccaacattc gggccggcca
     7861 ccatattatc ggaacggact tcgccccaga ccaacatgcg ttctatacag gccggctcta
     7921 tcaaagagcg gtaacaggac gccaccacct gctccacatg agcaccttct ttccaataat
     7981 cttcgagtat cagctctcct tcgggaaaaa tggtcaacca atcctgacag gaattcatac
     8041 ccaggcttaa caaaacggcc aggaatgcca cttttatttt atgaaatgct gttttcattg
     8101 ttcgatttaa ttaagcttag aaaccaattt ttatacccaa agtaaagtcc ttggggcgag
     8161 gtgttttact gctgtctttg gccaccgatc tggagccgta acccacctcg gggtcaacac
     8221 cggtgtagtt ggtaaaaaca agtagattct ggaaggtcag gtaaaaactc aaggtattca
     8281 atttatacgg tttgaggtat tgagtaggta ccgcgtaatt gaaagtcatg tatttaaagc
     8341 gtaaaaacga cgcgtcttct acgtagcggt cactgcccag ccagttatat ccgtagttat
     8401 acagagcacg gggcatctcc gtaacgtcgc cttccttgcg ccaacgccag ttgaccgcaa
     8461 tgctttggtt gttgttggtg tacatatttt ctgcttccat acgggcggcg tttaccactt
     8521 tattgcctgt acggaaatta aagaaaagat tgagggacaa ttgtttccaa cgcaaagtac
     8581 taccgaaacc tcccataaat ttgggcaagg aattgcccag gtaaacgata tccaattcat
     8641 ctatagtgcc gtcgtgattg atgtcttcgt aaatggcatc gcccgcttta aattcgtact
     8701 gggccgaacc gccgtaagca taatacatag gcttggtttt gccgtcggca tcaaaaataa
     8761 cattgccttc tttatcgcga actacagggg cactctcctg aacaccttct atgtaatcgt
     8821 tgtactgata gacccctttg tagcgaaaac cgtagatgga accgtaggga tttctttcct
     8881 gaatacgcgt caggtattct ccgttggagt aggtataatc gggattcatg gtagccagga
     8941 tgtcgtcgtc caattcaacg atgatattcc ggttgttgga tgtgttgaaa ttgaaggaca
     9001 ttctgaaatt ccccgcttta accaggtcgc gcacatgtaa attggcttcc caaccgctgt
     9061 tgttcatagt ccctccattg cgggcactta attgagcata gcctgtatga ctgggtaagc
     9121 cgattttgtt gaacaacaaa tcggtggtat gcttcatgta atagttcaga tcgatggcat
     9181 atttcccctt caaaaattcc aggtcggcac ctaagttgat tgaatttgtt ttttcccacc
     9241 tcaggtcggt gagcctgata ttgaaaggat atacggcaat catgtcgata taacccttat
     9301 cgtagctctt gtaccgactc aagtgcaggt attcggtaga gggctggtta cccccgatac
     9361 cccaactggg gcgaaaagca aacatattga cgatgtgtct gtaatcgtcc caaaaaggct
     9421 cgtccgatat aatccacttg cccgaaatgc cgggaaacaa accaaattta aagttatcac
     9481 caaaacggga gcttccgttg aagttggccg taaaatcaaa aatatagcgc cccttgtacg
     9541 catagtgtcc gctccaactg taacttaatc cgcggctttc actggttgaa ctgctggttg
     9601 agcccaaaat gccgggcacc gttgcatcac ggatgatggt agaaggaagg ccataggcgg
     9661 aaatacccag agatcccgaa gttcccgtat taacacggaa attggcgcgc agcatcaggc
     9721 tgtggtcgcg attctcaaaa gccggccgat agtcaacact ggtggagaat tgaacatcga
     9781 ggctctcgtc ttcggaatta ctggtcaggt ttaccttatc gctattccaa cgggcattgg
     9841 aaatatcgct gggtagaaaa gtactttttt ggctgttccc caaactaaag gacacggttg
     9901 aattaaatct caacgttgaa ttgtacttat agggatccag caaatcgtaa cgcagtgaaa
     9961 aattcggcga aatacgataa cttttggtgt tgtttttggc taacataccc accgctaccg
    10021 gattgctcag gtttttctga ctatcgtgta gttgagagtc tgtacgaatg ttgtaataca
    10081 cgtccgtatc gttcccgtaa aggtcttgct cataaatact gacgttgggc atttttttgt
    10141 aggcattgcc caaaaggttt tcgtagttcc tgtcgttgtt tgaatacgta taggcaaagt
    10201 ccgaagtaaa taaaatacgg tcggaaacac ggtattctat gctggagcgg gtggaaaaac
    10261 gttccagcaa ctgaccgata accgttcctg tttgactgta atagccggcc gatacgcgga
    10321 aagtgatttt ttcgccccct cccgaaatag tcaggttatt gtcgtgcgta aaaccatagc
    10381 ggctgactgc atctatccag tcggtattat tgttgaaatt ttcgtattcg gtaaaattgg
    10441 tgtcgtaatt taattcgggt atgccggcag aaaggttttc caaggtggga ttgtaatacg
    10501 attgcttcat gagcatcgta tagtcgtctc cgttcaacat ttttaagccc ttaggctggc
    10561 gggaaccgga aagccggtag gaataagaaa cacgcgttgg gccgcgttga cctcttttgg
    10621 ttgtaatctg caaaacaccg ttggctccct gcgatcccca gatggccgtc gaagccgcgt
    10681 ccttcagaat cacaatggat tcgatatcgt ccggattgac gtcgatcaac tgggcgtact
    10741 g
//
LOCUS       DAOSGL010000040        10712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5198_length_10712_cov_3.986957, whole genome shotgun sequence.
ACCESSION   DAOSGL010000040 DAOSGL010000000
VERSION     DAOSGL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5198_length_10712_cov_3.986957"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKY68_02345"
     CDS             complement(<1..434)
                     /locus_tag="PKY68_02345"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOH23912.1"
                     /translation="MSRLYSTLTVLFFSLTIYNRAEPALYRVAETKPGSGCYVMQNSR
                     EQLIGQAFQQDALVSYRGWQYTVYYNLERKVCIARRKWPAGQWDEVILSYKNNRDDAH
                     NVITMGICKKDGSIHLACDHHNDPLHYCRSVTGLANDDKLAWT"
     gene            613..4617
                     /locus_tag="PKY68_02350"
     CDS             613..4617
                     /locus_tag="PKY68_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOH23913.1"
                     /translation="MQISFEYFPYMDQLPSSSVRRVLQDSEGYMWFGTLDGVCRYDGY
                     QVRTFRSDMHNPSLLTNNEVQSLAEDKQNRIWIGTKQGINLLDRNTLNITPFDESPVQ
                     GDNINALACDDKGNMWIATPRGLFCYDNTRNCLKTYWSDPYDEKNLPDQTISHVSKDK
                     QGNVWIMFWNGGLCRYLPESDNFERFPPTKEMSGPFRIYQDKNDQYWLCTWGSGFFKF
                     DPEAPPENRYTRIPVYNTAGEEENILFSMVQDDRYGYFWLMSLTGLYVFDYDEQNTRL
                     NPVDISEHLIGSSKLYSEIFKDKDNNLWIGAYSEGAIFVNFDLPNVRNYGMEVLRQKL
                     GFPVSIRAMSEDSDGLVWLGVNRYGVFFYDRHENRVFSIDEIPTIENHQELSNLKTVN
                     YIREIKSRGEHWICCDDQWVYIFRKDGRGVYYAGRLDIDVSNELNWASGKVLFEDKDS
                     CVWVGMLNQGLVKISPENRDTMVAPIPMVTGITQDLHGDIWVSSEKVGLCCLKPSGET
                     YRLIRYDKYSEGLNTNNIQSVCADLTGKLWLGTKEGRLLTFDKDSGSFEDMSLACAMT
                     GESILNLLVDRNNNLWISTNKEVTGFNHRTLTSTSYSISDNLVVKSFIKGSYSATSTE
                     EVLFGGNKGFCAFSPIRQHNDSLKSKSVRVHITDIKVHNQSVLSNNDEHGYEPELKRL
                     ILGPAERNVEFEFSSLNYAAPFKVQYAYKLEGLDPEWTKVNATRRFALYNNLHQGKYK
                     LMFMATDENGRWSEPLPGLEIIKRPPLYLSWWAILLYVVLLMLLFYALIRFFTNRVRL
                     NQQLNIAQIEKAKSEELTQTKLRYLTNVSHELLTPLTVISCLVEDVDQVSKGEYWQHE
                     VMKVNLSRLKRLLQQILDFRKVESGSMKLKVMKSNMIEFVERICAFNFVPLAKERDIR
                     FELQLPDYPVQGWYDPEKLDIILFNLLSNAFKFTPEGGSVRLSLSMLQKNKGRAAQLI
                     VSDTGRGIQPDELEQIFTRFYSNDSSLTVENHGIGLTLTKELVEMHHGSIRVESEPGK
                     GSTFILEFPIEKSAYAPEELVAETTEEEAVLSDQDSDTGKSQASTKHKEDVHILIVED
                     NAQLCDTIEAILSRNYHTYKAFNGKEAVALINSRHIDLVVSDIIMPEMDGLELCRTIK
                     NNLDTSHIEVLLLTAKNSINDRIESYNAGADGYLSKPFELKVLQAKINSLIRNRKRKM
                     DSFKHNAEINISSLEYTSLDEKFLENAVSIVEEHIAVTDFNLDLLSSRLNMSKSSLYR
                     KIKSLTGLSPVEFTKNIRLKHACQMLTDRAGNISDIAYAVGFADPKYFTSCFKAEFGL
                     TPTEYIKKIKKEAR"
     gene            4801..5898
                     /locus_tag="PKY68_02355"
     CDS             4801..5898
                     /locus_tag="PKY68_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOH23914.1"
                     /translation="MLLLSCRAKDRDCIRPGEIWPDNNGVHINAHGGGMLYHRGQYYW
                     FGEDKSERSNAALKGVRCYSSKDLCHWKDEGIVLAVVEDEPAHELTKGCIIERPKVVY
                     NKKTGTFVMWFHHELRWRGYSAARVGLAVSDKVTGPYRFIHSYRPNPGIWPENMSEED
                     KSLDMEEASRLESWTPEWLEAVRKGFFVIRDFEGGQMSRDMTVFVDDDGKAYHIYSSE
                     ENLTLHIAELSYDYQSHTGKYIRIIPAGHNEAPAIFKKDGRYFMITSGCTGWRPNAAR
                     LLTATSIWGPWEQKPNPWQGEQADISFDSQSTFIFPLAGKKDAFVFMADRWRPRNPSD
                     GRYIWLPLEFEDGLPVLRWRDSWSPAKAFKK"
     gene            5907..7706
                     /locus_tag="PKY68_02360"
     CDS             5907..7706
                     /locus_tag="PKY68_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH23915.1"
                     /translation="MKKSLLIYLLILGGFSLYGRGTPPYVLQNVYARNYETLNGSWNH
                     IVDPFDNGYYDYRMNINRSGFFRNQKQANPSDLVEYNFDTSPCLQVPGDWNTQDDRLF
                     FYEGSVWYKRDFNFEPREGERQFIYFGAVNYFCNIYLNGREIATHEGGFTPFSVEVTD
                     KLREGHNFLILRVNNDRHPENVPTVNFDWWNYGGITRDVLLVSLPKLFVDDYSLRLKK
                     GSYRELEGYVSLSDPQAGVELSLEIPELKIKQTIQTGEQGRADFNIKAKPELWTPQNP
                     KLYRIILRQGNEVIEDNIGFRHITTEGKKILLNGQQVFLSGISIHEEAPYRQGRVYSK
                     EESVLLLSWAKEMGCNFVRLAHYPHNEHMVRAAEEMGLMVWSEIPVYWTIAWDNQDTY
                     QNASNQLRDMMDRDKNRCAVVVWSVANETPHSEARDKFLIGLANQVREKDDSRLLSMA
                     MEVTGQGNNISRVRDNMSDYVDIISVNTYLGWYGGSLDAILSRTWEIPYDKPVFFSEY
                     GAGALQGLHGEENVRWTEEYQERLYKNTIEMFDKVEGFSGMSPWILMDFRSTRRQLRG
                     VQDFFNRKGVISERGIRKKAFYVLQNYYKSKDY"
     gene            complement(7791..8798)
                     /locus_tag="PKY68_02365"
     CDS             complement(7791..8798)
                     /locus_tag="PKY68_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOH23916.1"
                     /translation="MNLDELNYCPGTLAEGFSTYSPSCLRHLFGGKKVSHVLPYEQPQ
                     QSEKVAAQFIENRKRISISGVQEKLSFLLDKNVLRLTKEGEQGKYILKPVPQDLKKVD
                     QVPANEHLTMQIAKQVYGLNTAENALIFFKDGSPAYITKRFDVKEDGFKWGKEDFATL
                     AGKTKDNSGVNFKYDYSYEELGQLIRTFVPAWRIEIEKYFSLVVFNYLFSNGDAHLKN
                     FSLLESSSGDYLLSPAYDLINTRLHTDDTDFALDGGLFADEYKSEQYKKRGHPSKSDF
                     TEFALRIGVTESRVGKLLNPFLEKQVFVETLIARSFLTDANKRAYLLMYNTKRNYLTA
                     Q"
     gene            complement(8795..9124)
                     /locus_tag="PKY68_02370"
     CDS             complement(8795..9124)
                     /locus_tag="PKY68_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HOH23917.1"
                     /translation="MRTMEIYRNGILAGILTEEHRQRYVFRYDDNYFNDSSKPGISLT
                     LPKTQQEYCSDCLFPFFFNMLSEGVNRKLQSKLLRIDEEDHFGLLAATAQYDTVGAIT
                     VKSMEAR"
     gene            complement(9453..10346)
                     /gene="atpG"
                     /locus_tag="PKY68_02375"
     CDS             complement(9453..10346)
                     /gene="atpG"
                     /locus_tag="PKY68_02375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOH23918.1"
                     /translation="MSSLKEIKNRIHSVQSTRKITSAMKMVSMAKLNKTERIIGGIHS
                     FYHHLHDILKVILDDGKDYQTDFSEARELKRIALVVFSSNSSLCGSFNSNIAKRLNVE
                     IRNYASLGRENILVIPIGKKIAQASVKMGYNIPSGFENLDFILDKPNAKVVLDLANQL
                     KQLYLSGKVDKIELIYHHAKSKSSQVLEARTLLPLDLKERDVIEKPVVRERIYEDDFI
                     IEPDRENLLHDLVPYIINMRLYLALLDSSVSEHTARMLAMQTATDNADELLEEIKLQY
                     NKLRQQAITSELLDIVGGTME"
     gene            complement(10346..>10712)
                     /locus_tag="PKY68_02380"
     CDS             complement(10346..>10712)
                     /locus_tag="PKY68_02380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445947.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOH23919.1"
                     /translation="LKIDQAQYRELEAFSKFGSDLDAVTAMTIDKGRKNERLLVQPVH
                     SPIEVEKQIAVLYCGTKGLLKDLALIKVDAFKKEFLARLESLHKENVLNPIRKGIFND
                     EIIAVIEQVAADVILSLSV"
BASE COUNT         2903 a         2390 c         2462 g         2957 t
ORIGIN      
        1 gtccaggcaa gcttgtcgtc gttggccaga cctgtaaccg agcggcaata atgtaaagga
       61 tcgttgtggt ggtcgcaggc caggtgaatg ctgccgtcct ttttgcagat acccatagtt
      121 atgacgttat gggcgtcgtc ccggttgttt ttgtaggaga gtatcacttc gtcccattgg
      181 ccggccggcc atttgcggcg agctatgcac acttttcgtt ccagattata ataaaccgtg
      241 tactgccaac cccggtaact caccagggca tcttgctgaa aggcctgtcc gataagttgc
      301 tcccgggagt tttgcataac gtagcagccc gaacccggtt ttgtttcggc tacccggtag
      361 agggcgggtt ctgctctgtt gtaaatagta agggagaaaa aaagaacggt aagtgtagag
      421 tacaatctgg acataataca gttatataac gctttaagta tcatttctcc ggcgaaggta
      481 cggttttatt ttattttgag aaatacggct ttttgattta ctttgtacac aaacacccga
      541 gataccatgt ccgcccgcct tactggatta cttgtttttg caacactgct tgtttttccc
      601 ttgcggggag aaatgcaaat ttcatttgaa tactttcctt atatggacca attgccttcc
      661 agttcggtta gaagagtatt gcaggacagc gaaggttata tgtggtttgg cactctcgac
      721 ggggtttgcc gttacgacgg ctatcaggta agaaccttcc ggtccgatat gcataatcct
      781 tcgctattga ccaacaacga ggtccaaagt ctggccgaag ataaacaaaa ccgcatttgg
      841 ataggcacta agcaaggaat caatcttttg gaccgcaaca ccctgaacat tactcccttc
      901 gacgaatccc ccgtacaggg agacaacatc aatgcactcg cctgcgacga taaaggcaat
      961 atgtggattg ccaccccgcg gggtttgttt tgctacgaca acacccgcaa ctgtttaaaa
     1021 acttactgga gcgatcctta cgacgaaaaa aacctgcccg atcagaccat aagtcatgtt
     1081 tcaaaagaca agcagggcaa cgtctggatt atgttctgga acgggggctt atgccgctac
     1141 ctgcccgaaa gcgacaattt tgaacgtttt ccgcccacga aagaaatgag cggccccttt
     1201 aggatttatc aggacaaaaa cgatcagtac tggctctgca cctggggcag cggtttcttc
     1261 aaattcgatc ccgaagcccc gcccgaaaac agatataccc gcattcccgt gtacaacacg
     1321 gcaggggagg aggaaaacat cttgttcagt atggtgcagg acgatcgcta cggttatttt
     1381 tggttgatgt ccctaaccgg tttgtatgtt tttgattacg acgaacaaaa taccaggttg
     1441 aatccggtag atatctccga acatttgatc ggatccagca agctgtacag cgagatattc
     1501 aaagacaagg ataacaattt atggataggc gcctacagcg aaggagccat ttttgtgaat
     1561 ttcgatcttc ctaatgtgcg taattacggt atggaagtgc ttcgtcaaaa gctgggtttc
     1621 cccgtaagta tcagggcaat gtcggaagac tccgatggtt tggtctggtt gggggtaaac
     1681 cgttacggtg tgttttttta cgatcgccac gaaaacaggg tattcagcat tgatgaaata
     1741 cccaccattg aaaaccacca ggaattgagc aacctgaaaa ctgtaaacta tatccgcgag
     1801 ataaaaagtc gcggagagca ttggatttgt tgtgacgacc agtgggtata tatattcaga
     1861 aaagacgggc ggggcgtata ctatgccgga cgtctcgaca tagacgtcag taatgaattg
     1921 aactgggcct cgggaaaagt gctttttgaa gacaaagact cttgcgtctg ggtggggatg
     1981 ctcaatcagg gattggtcaa aataagtccc gaaaaccggg ataccatggt ggcacccata
     2041 cccatggtaa caggtataac tcaagacctg cacggggaca tttgggtaag tagcgaaaaa
     2101 gtgggcctct gttgtctgaa accctccgga gagacttacc ggctaattcg ttacgataag
     2161 tattctgaag gattgaatac caataatata caatctgtct gtgcagatct caccggtaaa
     2221 ctctggcttg gaaccaaaga gggccgtctg ctcacgtttg acaaagacag cggcagcttc
     2281 gaagacatga gcctggcttg tgccatgacg ggtgaaagca ttctgaattt gttggtcgac
     2341 cggaacaata atttgtggat atctaccaat aaagaagtga ccggtttcaa tcacagaacc
     2401 ttgacctcta cttcatacag catttccgac aacctggttg tcaagtcttt tatcaaaggt
     2461 tcttactctg ccaccagcac cgaggaggtg ttgttcggag gcaacaaagg cttttgcgcc
     2521 ttttcgccaa tcaggcagca caacgacagc ctcaaaagca aaagcgttcg ggtacatatt
     2581 accgacatta aagtacacaa ccagtcagtt ttaagcaata acgatgagca cggctacgag
     2641 cccgaactca aacggctgat tttgggacct gcggaacgca acgtggaatt tgaattttcc
     2701 agtctgaatt atgcggcacc gtttaaggtg caatacgcct ataaattaga agggctcgac
     2761 ccggaatgga ccaaagtgaa cgctacccgg cgctttgcct tgtacaataa tttacatcag
     2821 ggcaagtata agctcatgtt tatggcaacc gacgaaaacg gccgctggag tgagccgctt
     2881 cccggcctgg aaatcataaa gaggcccccc ttgtatctga gttggtgggc aatcctgctg
     2941 tatgttgttt tgctgatgct gttgttctat gccttaattc gtttttttac caatagagta
     3001 cgtctgaatc agcaacttaa tattgctcaa attgaaaaag ccaaatccga agaactcact
     3061 caaactaagc taaggtattt gactaatgtg tcgcacgaac tattgactcc tctgacggtg
     3121 atttcctgtc tggtcgaaga tgtggatcag gtatcgaaag gcgaatactg gcagcacgaa
     3181 gtgatgaaag tcaatttaag ccggctcaaa agattgttac agcaaatact tgatttcaga
     3241 aaagtagaaa gcggctccat gaagcttaaa gtgatgaaat cgaacatgat cgaattcgtg
     3301 gaacgcattt gtgcttttaa ttttgttccc ctggccaaag aaagagacat ccgttttgaa
     3361 ttgcagcttc ccgactatcc cgttcaaggc tggtacgatc ctgaaaaact ggatattatc
     3421 ttattcaacc tgctttcgaa tgcttttaag ttcacgcccg aagggggatc cgtgcgcctt
     3481 agcctttcca tgctgcaaaa aaacaaaggc cgtgctgctc agttgatagt cagtgatacc
     3541 ggccggggta tacaacccga cgagttggag caaatattca cccgttttta ttcgaacgat
     3601 tcaagtctta cggtcgaaaa ccacggtata gggctgactt tgaccaaaga acttgtcgaa
     3661 atgcatcacg gcagcatcag agtcgaaagc gaacccggca agggaagcac ctttatcctg
     3721 gagtttccca tcgaaaaatc ggcctatgcg cctgaagaac tggttgctga aaccaccgaa
     3781 gaagaagccg ttctttccga tcaggacagc gataccggta aatcgcaagc ttcaactaaa
     3841 cataaggaag acgtgcatat cctgatagtg gaagacaatg cgcaactttg cgataccatc
     3901 gaagccattt tgagcagaaa ttaccatacc tacaaggcgt tcaacggtaa agaagcagtt
     3961 gcccttatca acagcaggca tatcgatttg gtagtcagtg atattattat gcccgagatg
     4021 gacggcctgg aattatgccg tactattaaa aacaatttgg ataccagtca tatcgaagtg
     4081 ttgttgctga cggccaaaaa ttccatcaac gaccgcattg agagttacaa cgcgggtgca
     4141 gacggatatt tgtccaaacc ctttgaattg aaagtcttgc aagccaaaat caacagcttg
     4201 attcgcaacc gcaaacgcaa gatggattct ttcaaacata atgccgagat caacatttcc
     4261 tcgctcgaat atacttcgct cgacgaaaaa ttccttgaaa atgccgtgtc tattgttgaa
     4321 gaacacattg ccgtaaccga ttttaacctc gatcttctat cgagtcgact gaacatgtcc
     4381 aagtcgtcgc tttaccgcaa aatcaagtcg ctcaccggcc tctcgccggt agaatttacc
     4441 aaaaacatcc gtctgaaaca cgcctgccag atgctgaccg accgggccgg aaatatttcc
     4501 gatatcgcct atgccgttgg ctttgccgac cctaagtatt tcacttcttg ctttaaagcc
     4561 gagtttggtt taacccccac cgagtacata aaaaagatta aaaaggaagc ccggtaaaaa
     4621 aggacttaat ttttgcggct tagtcatatt ggcagaataa tcaccattaa tgaacaattt
     4681 ttggtattat aagctgttgt ttgcttctac ttttgtcaat caagagtctt tagttatatg
     4741 acaacgtccg aattttttac gagaaggttt ttgcgcccgg tcctgttttt ctcttttaca
     4801 gtgctactgc tttcgtgcag ggccaaggat cgggactgca ttcgtcccgg agaaatctgg
     4861 cccgacaata acggagtaca tatcaatgcc cacgggggcg gtatgctgta tcaccgggga
     4921 caatattatt ggttcggcga agacaagagc gagcgcagca acgctgctct caagggcgtt
     4981 cgttgttatt cctcgaaaga tttgtgtcac tggaaagacg aaggtattgt gcttgctgtt
     5041 gtagaagacg agcccgctca tgaattgacc aaaggctgta tcattgaacg ccccaaggtg
     5101 gtttacaaca aaaagaccgg cacttttgta atgtggtttc accatgaatt gcgttggcga
     5161 ggctacagcg cggcgcgggt gggtttggcc gtgagcgaca aggtgacggg accttaccgt
     5221 tttatacatt cctacaggcc taatccgggc atctggcccg aaaacatgag cgaggaagat
     5281 aagtcgctcg atatggagga agcctcccgc cttgaatcct ggactcccga atggttggag
     5341 gccgtcagga aaggcttttt tgtaatccgc gatttcgaag gaggacagat gtccagggac
     5401 atgaccgttt ttgttgacga cgacggcaaa gcctatcata tctattcttc cgaagaaaac
     5461 ctgactctac atattgccga actcagttac gattatcaga gccatacagg caaatacata
     5521 cgtatcatac cggcagggca caatgaagct cctgccattt tcaaaaaaga cggacgctat
     5581 tttatgatca cttccggttg caccggctgg aggcccaacg ctgcccgcct gctgacggct
     5641 acttccattt ggggaccctg ggagcaaaag cccaatccct ggcaaggtga acaggcagat
     5701 atttctttcg attcacaaag tacttttata ttcccgctgg ccggcaaaaa agacgctttt
     5761 gtctttatgg ccgatcgctg gagaccccgg aatccatcgg acggacgtta tatttggttg
     5821 ccgcttgaat ttgaagacgg gctaccggta ttacgctgga gagacagttg gtctcctgct
     5881 aaagctttta aaaaatgaat agagttatga aaaaatcctt gttgatttat ttgttgattt
     5941 taggagggtt cagcttgtat gggcgaggca ccccccccta tgtcttgcag aatgtgtacg
     6001 cccgcaatta cgagactctg aacggatcgt ggaatcatat agtggatcct ttcgacaacg
     6061 gttactacga ttatcgcatg aacatcaacc ggagcggttt ctttcgtaat caaaaacagg
     6121 ccaatccttc ggatctggta gaatacaatt tcgatacatc gccttgcctg caagtacccg
     6181 gtgactggaa cactcaggac gaccgcctgt ttttttacga aggcagcgtt tggtataaac
     6241 gcgatttcaa ctttgagccc cgggagggag aaaggcaatt tatctatttt ggtgccgtca
     6301 attatttttg caatatatat ctgaacggta gggaaatagc cactcacgaa ggaggattta
     6361 ccccctttag cgtcgaggtg acagacaagc tcagagaagg acataatttt ctgattctaa
     6421 gagtaaacaa cgatcgtcac cccgaaaacg tgcccaccgt caatttcgat tggtggaatt
     6481 acggaggcat cacccgagac gtgcttttgg taagtttgcc caaactgttt gtggacgatt
     6541 attcgttgag gctgaaaaaa ggaagctaca gggagctgga aggctatgtt tctttgagcg
     6601 atcctcaagc aggagtggaa ctgagcctcg aaattcccga actgaaaatc aaacaaacca
     6661 ttcaaaccgg agagcagggc agggcagact tcaacatcaa ggccaaaccc gaattgtgga
     6721 cgccccaaaa tcctaaactc taccggatta tcttgcgtca gggcaacgaa gttatcgaag
     6781 acaacatcgg attcagacat atcactaccg aaggcaagaa aatattgctc aacggccaac
     6841 aggtcttttt gagcggtatc tccatacacg aggaagcccc ctaccgtcag ggcagggttt
     6901 attccaaaga agaatcggtt ttgctcctgt cgtgggccaa agaaatgggc tgtaattttg
     6961 tacgtctggc ccattatccg cacaatgaac atatggtgag ggctgccgaa gaaatgggtc
     7021 tgatggtatg gtcggaaatc cctgtttatt ggaccatagc ctgggacaac caggacacct
     7081 accaaaatgc ctccaatcag ctccgggata tgatggatcg cgataaaaac cgctgcgccg
     7141 tggtggtttg gtcggtggcc aacgaaactc cacatagcga agcccgggat aaattcctga
     7201 taggcttggc taatcaggtg cgcgaaaagg acgacagccg cttgttgagc atggctatgg
     7261 aagtcaccgg ccagggcaac aacatcagcc gggtacgcga taatatgagc gattacgtag
     7321 atatcataag tgtaaatact tatctcgggt ggtatggcgg ttccttggat gccatattaa
     7381 gccgcacctg ggaaatacct tacgataaac cggtgttttt cagtgaatac ggtgcgggcg
     7441 ccttgcaagg tctgcacggt gaagaaaacg ttcgctggac cgaagagtac caggagcgtt
     7501 tgtataaaaa taccattgaa atgtttgata aagtagaggg attttccggt atgtcccctt
     7561 ggatattgat ggatttccgc tctacgcggc gccagttgag aggcgtccag gattttttca
     7621 atcgtaaggg agtgatctct gaacgaggaa tcaggaagaa ggctttttac gtgttgcaaa
     7681 actactataa aagcaaggat tattaagggt taggactgat gccggtcttc tgtgttgaac
     7741 gccggcagac tatcaaggaa ggctgctcca tcgggcagcc ttctttatga tcattgagct
     7801 gttaaataat ttcttttcgt gttatacatc agcaagtaag cccttttgtt ggcatcggtc
     7861 aaaaacgaac gggcaatcaa tgtttccaca aaaacctgtt tttctaaaaa cggatttaat
     7921 agtttcccga cccggctttc tgtaactcca attctcaggg caaattctgt gaaatcgctt
     7981 ttggaaggat gtcctctttt cttatattgc tcgcttttat attcgtctgc aaacagccct
     8041 ccatccaatg caaaatccgt gtcatcggta tgtaatcttg tgtttattag atcgtaagca
     8101 ggacttaata gataatcccc gctggatgat tcgagcaagg aaaagttctt taagtgggca
     8161 tcaccatttg aaaacagata attgaaaaca accagagaaa aatatttttc tatttctatt
     8221 cgccacgccg gtacaaaagt cctgatcaat tgtcccagtt cttcgtagct gtaatcgtat
     8281 ttgaaattta cacctgaatt gtcttttgtt tttcctgcca gcgtcgcaaa gtcttctttg
     8341 ccccatttaa atccgtcttc ttttacatca aatcgcttgg tgatataagc gggtgaacca
     8401 tccttaaaaa aaatcagagc gttttctgcc gtattcaagc catacacttg tttggctatt
     8461 tgcattgtca ggtgttcatt ggcgggaacc tggtcaacct tttttaaatc ttgtggcacc
     8521 ggtttgagta tatatttccc ctgttctcct tcctttgtta atcgtaatac atttttatcc
     8581 aagagaaaac tcagtttttc ctgcacaccc gatatggata tgcgtttgcg attttctatg
     8641 aattgtgccg ccactttttc gctttgctgg ggctgttcgt agggcaaaac atgactcacc
     8701 tttttaccgc cgaataaatg acgtaaacag cttggactat aggtagaaaa accctcggcc
     8761 aacgttcccg ggcaatagtt taattcatct aagttcatct cgcttccatt gattttacgg
     8821 ttattgcccc aactgtgtcg tattgcgctg ttgctgccaa tagaccgaaa tgatcttctt
     8881 cgtctattct caatagctta ctttgtaatt tccggtttac accttcgctc aacatattga
     8941 aaaaaaatgg aaacaagcag tcgctgcagt attcttgttg tgtttttggt agcgttaaac
     9001 tgattcccgg tttgcttgaa tcattgaaat agttgtcgtc atacctaaaa acataacgtt
     9061 gacgatgttc ttctgttagg attcctgcca aaatcccgtt gcggtaaatt tccattgttc
     9121 tcatttactc aaggtctttt ttttacttca aggctgagtt tcattcccaa tacttccgat
     9181 aatctattaa ttatatctaa cgaaggattt ccttgccctc gttctaattt gtataaggta
     9241 tttatgctca ctttggccaa ttcggccaag tggggttgag ttatgttcaa ctccttacgt
     9301 ctgtttttta tggtttcgcc gagcgtctta actaacatta taatgtgatt ttatggtcaa
     9361 aagtaggact cctttctcaa atatacaagc aaaatcacat tatattggta aaaatacttg
     9421 ttcgtttggg gtcaaaagct gataagtaaa tcctattcca tcgttccccc tacgatatcc
     9481 aacaactcgc tggtgatggc ttgctggcgg agcttgttgt attgtaattt gatttcttcg
     9541 agtaattcat cggcattgtc ggtggcggtt tgcatggcca gcatacgggc tgtatgctct
     9601 gatacgctcg aatccaacaa tgctaaatac aggcgcatat tgatgatgta gggcaccaaa
     9661 tcgtgcaata aattttccct gtcgggctcg atgataaagt cgtcttcata aatacgctct
     9721 ctgacgacgg gtttttctat tacatccctt tctttcaaat ccagaggaag cagtgtgcgt
     9781 gcctccaaaa cctgcgagct cttgcttttg gcgtggtggt agatgagttc aattttatca
     9841 actttaccgc ttagatacaa ttgtttcagt tgattggcta aatcgagcac gactttcgca
     9901 ttgggtttgt cgaggatgaa gtccaaattt tcaaaaccgg aggggatgtt gtatcccatt
     9961 tttacgctgg cctgggctat ctttttccca atggggataa ccaaaatgtt ttcacgtcct
    10021 aaagaggcat aattccgaat ttccacgttc agtcttttgg caatattgct gttgaagctg
    10081 ccgcaaagac tggagttgga tgaaaacaca accagggcaa tgcgtttcaa ttcacgtgct
    10141 tcggaaaaat ctgtctgata gtccttacca tcatccaaaa tcacctttaa gatatcgtgt
    10201 aaatgatgat aaaatgaatg gatgccgcct attatcctct ctgtcttgtt gagcttcgcc
    10261 atggagacca tcttcattgc agaagtgatc ttccgtgtgg attgcacgga atgaatgcgg
    10321 ttttttattt ctttcagtga ggacattata cagaaagact caggattaca tctgctgcca
    10381 cttgctcgat gacagcaata atttcatcgt taaaaatacc ctttcgaatg ggatttaaca
    10441 cattctcttt gtggagagat tccagcctgg ctaaaaattc ttttttgaag gcatctactt
    10501 ttatcaaggc cagatctttc aacaaacctt tggtaccgca gtacaggacg gcgatttgct
    10561 tttcaacctc gatgggagaa tggacgggct gtaccaacag acgttcgttc ttacggcctt
    10621 tgtctatggt catggcagta accgcatcca aatcactacc gaacttggag aaagcttcca
    10681 attctctgta ttgagcctgg tctattttca gg
//
LOCUS       DAOSGL010000041        10711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5199_length_10711_cov_4.565972, whole genome shotgun sequence.
ACCESSION   DAOSGL010000041 DAOSGL010000000
VERSION     DAOSGL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5199_length_10711_cov_4.565972"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="PKY68_02385"
     CDS             <1..1490
                     /locus_tag="PKY68_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOH23920.1"
                     /translation="LTGGSYSFTTCSPKAKVFDAVVAKDGSGDYTSIRQAVKAAPNNR
                     TEPWLIFVRNGVYEELVRTSTSQPNICLVGEDKEKTVLKFSITSYGGHERNNSLFPEA
                     EGQGPVLVANSSDFYLHNITVINSWGYDNQAGPQALALGSYADRFTMFNAVLKSYQDT
                     WQTGRDEHRHYAFRSYIEGAVDFIYASGNCVFDSCTIALCRNGSVVVAPSHGAGVKYG
                     YVFRDCNVVSSKPGTARTNNYWGRPWHNRPRTSFINTSLSKDINLSPAGWIDHMGGLP
                     VVFAEYNTIDHMGNPVSLASRNTYYWTGSDRNNPTETCIAQAVLSKEEADALTVRTVL
                     SGTDGWKPDNITTELPAPLLYYSENRLYWTAEPQAICYEVLYNGDFLTFTTETSLTFE
                     GDVSGYRVRAVNMYGTLGQVSDPPFVNGIQTETAGALNVVFDGSRLLASGFCGKARVE
                     LYAIDGSKAGQYEIEENVRLDLGSVRFVIAKVTANTGTCVLKAVR"
     gene            complement(1549..5835)
                     /locus_tag="PKY68_02390"
     CDS             complement(1549..5835)
                     /locus_tag="PKY68_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH23921.1"
                     /translation="MKATSSLRKSLFAYIIAFFLTPAEFSLSANSRVKPFVTYNHYSS
                     DDEFLQGSITYLLQDRRGLIWVSSFNGLNKYDGYQWTKYKSRPGEKSLLNTNRINYLW
                     ENSSGNFWCEAGNQIHLFDVEKEEFTNLQTVFEQKYLRNVTIESIFPLGNGVSYMIGE
                     MGECFRIVDSDPLNKCREIAIKASASKDVFLVFLDSQGFEWILTKSGTYRYDTDQLVS
                     KIPFRFPAVTEDELWLTSADGRQIGIYSYQNANITYLDFIPFNGYVYKQHFKDSLIYT
                     ATGAGFYLTSIADRQTLCLSKEILQTFFVDSRGCVWGLTTGKEIIVINTKSDSGETAE
                     CQIVELPADHNFSDPRVSFYEDGSGRIWSIFYDTNDILYYNEGTGRFERPISPEGMIT
                     NTQGVAIDKQGNLWYRTNRHIDMLTLHNQPFEVINSFPGEEVRSMLKDSKDRYWFGLR
                     ENKVLCFRDNQLVHEIRIEGTAYCLYEDSNQRIWIGSKPGGLYCLEFTDGSDNYKLSR
                     YQHDDNDPKSLSHNDVYSIVEDSRGHLWIGTFGGGINLFKQGVFHHQNNHLGYDSELP
                     ETVRFICEIRPGLMLIGARNGLFSFNNLFDHPENIRMFRNTRRSLDINSLPDNDVMSI
                     LVTRDSSIYLTTSSGGICRILSGTDDLLSDRIRFESITKEDGLASDVAYSIIEDPQND
                     LWITGGRALSRLQRQEVSDNSGLQSPGIITIYDRTSFPSEVIFSEGHPLTEGSTLIFG
                     TMNGIVTIRSSQVLKDIYTPPLIVSSKSQDLKSKHQARAASIRFAALDYHDKLHLKYA
                     YKLDGVDKDWNITTENTVTYINLPPGNSLFHVKSTNADGIWTDNEQTMLIYVRPLFHE
                     TLPGRLILISAVLLFIALVVYYFQRMYRLRHRLILEQESTDTKLRFFTDISHELRTPL
                     TLIDGPVAEVLEDKQLSEQSRYYLEVVQKNVRRLLNLVNQILDFRKLQHNKITLLIEP
                     LDVKVLLSRIMENFAELANHHRILFNLNCPNDLPVIWADRDKMEKIFFNLLSNAFKYS
                     NDGGAISINVSKLNNSFNGDIEPSVVISITDQGKGIKREDVEQLFSRFETVIQDNLFR
                     PSSGIGLSLVKQFVDLHHASIGVDSQPGKGSTFSVTFRGGKKHFENDKNVEFYAGNLS
                     ENIQQEQPAESHQEDKAEEERSKILVVEDNEELRHFICRILAADYRVVCANDGEEGLR
                     MGKMEWPDLIITDIMMPKMDGFEMIRHIKADADIYAVPLIVLTAKGEMDDRIHGVELG
                     VDDYVLKPFSASYLKARVKALLEQRRQLQKHFLEILSQGGNALARRAIEPNMPVITPA
                     DELFIQEALAFMEKNMDNAELTIDQFADALNMGRTVFYNKLKTTLGLTPIDFVQEMRI
                     KRAVQLMKSSDYTIAEIAYQTGFNDPKYFSRSFKKHLGSSPSEYIRSIRK"
     gene            complement(5952..6134)
                     /locus_tag="PKY68_02395"
     CDS             complement(5952..6134)
                     /locus_tag="PKY68_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23922.1"
                     /translation="MKKILILLLKNLGIIIVLAGTVVLAATQFKGILTNTWLLVAAGL
                     FVLGLITQIFINKRVE"
     gene            6349..6693
                     /gene="rpsF"
                     /locus_tag="PKY68_02400"
     CDS             6349..6693
                     /gene="rpsF"
                     /locus_tag="PKY68_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOH23923.1"
                     /translation="MNNYETVFILTPVLSEPQMKEAVEKFKNLLVAEGAEIVNEENWG
                     LRKLEYPIDKKSTGFYQMFEFKANPEVVAKLEVNFRRDESVLRFLILKQDKFFAQYAE
                     KRRSLKQAKKEN"
     gene            6697..6966
                     /gene="rpsR"
                     /locus_tag="PKY68_02405"
     CDS             6697..6966
                     /gene="rpsR"
                     /locus_tag="PKY68_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOH23924.1"
                     /translation="MAQNSSEIRYLVPPTVDTKKKKYCRFKKSGIKFIDYKDPEFLKK
                     FLNEQGKILPRRLTGTSLKYQRKVAQAVKRARHLALLPYVTDLMK"
     gene            6981..7424
                     /gene="rplI"
                     /locus_tag="PKY68_02410"
     CDS             6981..7424
                     /gene="rplI"
                     /locus_tag="PKY68_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOH23925.1"
                     /translation="MEVILKEDVINLGYKDDIVKVKDGYGRNYLIPNGLAVMATESAK
                     KVLAEDLKQRAHKLEKMKADAQDMVSKFEGLHLSIGAKVSSTGKIFGSVGPIQIAEAL
                     EQQGLEVDRKSILLKEAVKEVGEYKATVKLHREVQVEISFEVVAE"
     gene            7663..8979
                     /gene="mtaB"
                     /locus_tag="PKY68_02415"
     CDS             7663..8979
                     /gene="mtaB"
                     /locus_tag="PKY68_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOH23926.1"
                     /translation="MTNQATTPSPTYLFYTLGCKLNFAESAGLGKKMQQVGYQPVKAG
                     REPDICVINTCAVTGTAEQKCRQNIHRLIRKYPKAFVVVTGCYAQLRAAELAAIPGVD
                     LVLGTDEKMDILTYLKSGKLEKAEVFSGPNSGIHRFFPACARGDRTRYFLKVQDGCDY
                     FCTYCTIPFARGRSRNIGIEEVVRQALEVAAEGGREIVLTGVNIGDFGKSNGEKLIDL
                     LRALDGVEGIERFRISSLEPDLLSDEIIEFVAASARFVPHFHLPLQAGSNEMLRLMHR
                     RYDREHFASRVAKIRQSMPGAFIGVDVIVGMRGETQERFEESRSFLESLDISQLHVFS
                     YSERPGTQALKIQPKVSSFELKRRSEILHSLSETKRLKFYGERRGEQAIVLFEKAKKN
                     NLIHGFTENYVRVEAPWRGDWENRSIPLTLGDFNADRSALQAVPIP"
     gene            9062..10036
                     /locus_tag="PKY68_02420"
     CDS             9062..10036
                     /locus_tag="PKY68_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH23927.1"
                     /translation="MGYVFLYGFCRLLAFLPLNMLYLLSDMILYPLVYYLAGYRKKVV
                     RTNLAASFPDKSDKERRSIEKDFYRHFCSVIVETIYLAGISTEEAKRRTSFINGELLE
                     KYLDRGQSVLVYLGHYGNWEYQTFLTLHYPGIHLINVYRPLTSKAFDRLMFKIRDRFD
                     AELLEKQAVLRKMVSLRQQGQAAVFGMIADQSPSKTNLHYWTRFLNQDTSVLTGVERL
                     AKSSGAAVVYGDVRVIKRGYYSMEFKLITDRARETADNEITEKYSRLMEESILRNPAF
                     WLWTHRRWKHKRNQAEKPAVGQIRESEEPAAGSTGIRYKSNAKWGPEI"
     gene            10042..>10711
                     /locus_tag="PKY68_02425"
     CDS             10042..>10711
                     /locus_tag="PKY68_02425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH23928.1"
                     /translation="METNNTKAVTNENANGGTGTKTKTGVNIKTTVVILNWNGKALME
                     QYLPVLIEHTSYPDVSLMVADNASTDASVEWLQTTYPGIQLLCLDRNYGFAEGYNRAL
                     AGLESEYLVLLNSDAKVTPNWLPPMIDYLDQHPGVAACQPKILNLRQPGYFEHAGACG
                     GFIDTLGYPFCRGRIQDYLEPDQGQYDSICDVFWTSGACLCIRREHFFAAGCLDGRFF
                     AHMEE"
BASE COUNT         2831 a         2374 c         2589 g         2917 t
ORIGIN      
        1 ccttgacggg cggtagctat tctttcacca cttgcagccc caaggcaaaa gtgttcgatg
       61 ctgtggtggc caaagacgga tccggagatt ataccagtat tcgtcaggcg gtaaaagcag
      121 cccctaacaa ccgtacggaa ccctggctga tttttgtcag aaacggggtt tacgaagagt
      181 tggtacgcac ttccacaagt cagccgaata tttgtctggt gggagaagat aaggagaaga
      241 cggtgttgaa gttctccatc acctcctatg gcggacatga acgcaataat tcattattcc
      301 ccgaggcaga aggccagggg ccggtgttgg tggccaattc ttccgatttt tatttgcata
      361 atattaccgt aatcaattcc tggggttacg acaatcaggc aggtcctcag gcgctggctt
      421 tgggaagtta tgccgatcgc tttaccatgt tcaatgccgt tttaaagagt tatcaggata
      481 cctggcagac gggtcgtgac gaacaccgtc attacgcttt tcgcagctac attgaaggag
      541 ctgtcgactt tatttatgct tcgggcaatt gtgttttcga cagttgtacc attgctcttt
      601 gtcgtaacgg ctcggttgtt gttgctcctt ctcatggtgc aggagttaaa tacggttatg
      661 tttttcgtga ttgcaacgtg gtttcgagca agccgggtac ggctcgtaca aataattatt
      721 ggggccgtcc ctggcataac aggccgcgaa cttcttttat caatacaagc ttgtcgaaag
      781 acatcaacct gtctccagcc ggttggattg atcatatggg aggcttaccg gtcgtttttg
      841 cggaatacaa taccatagat catatgggta atcccgtaag tctggcttcg cgcaatacct
      901 attactggac aggcagcgac aggaataatc ccacggaaac ctgtattgcc caggctgtac
      961 tgagcaagga agaagccgat gctctgacgg ttcgtacggt gctgagcgga acggacggct
     1021 ggaagccgga caatattact acagagcttc ctgcaccact tttgtattac agcgaaaacc
     1081 gtctttactg gacagcggag cctcaggcca tctgttacga agttctgtac aatggggatt
     1141 ttctgacatt cacaactgaa acatccctga cttttgaggg agatgtttcc gggtatcgtg
     1201 tacgtgccgt taatatgtac ggaacgctcg gacaggtttc agacccccct tttgtaaacg
     1261 gcattcagac tgaaacggct ggcgcgctga atgtcgtttt cgacggtagc aggcttttgg
     1321 caagcggctt ttgcggaaaa gcaagagttg aattgtatgc tatcgacggt agtaaagccg
     1381 gccaatacga aatagaagaa aatgtaagac ttgatttggg ttccgttcgt tttgttattg
     1441 ctaaggttac tgcaaacacg ggaacctgcg tgttgaaagc ggttaggtga atggtttaaa
     1501 gaatggagtg tgaggagggc gtcttattgt tgaggcgccc tcctttggtt atttccggat
     1561 ggatctgatg tattccgaag gacttgatcc gaggtgtttt ttgaaactgc ggctgaagta
     1621 tttgggatcg ttgaagccgg tttgataggc tatttccgca atggtataat cgcttgattt
     1681 catcaattga actgcccttt taatgcgcat ttcctgcaca aaatctatag gcgtaagacc
     1741 cagcgtggtc ttcagtttgt tgtaaaagac ggtgcggccc atattcaggg cgtcggcaaa
     1801 ctgatcgatg gtgagttccg cattatccat gtttttttcc ataaaggcca gggcttcctg
     1861 aataaacagt tcgtcggccg gagtaatgac cggcatgtta ggttcaatgg cacggcgagc
     1921 cagcgcattg cctccctggc tcagtatttc cagaaaatgc ttttgtaatt ggcgtctttg
     1981 ttcaagcaag gccttgacac gtgcttttaa ataggaggcg ctgaagggtt taagtacgta
     2041 atcatccact cccaattcta ctccgtgtat gcggtcgtcc atttcgcctt tggccgtcag
     2101 gacaatcagg gggaccgcat aaatatcggc atcggccttg atgtgccgga tcatttcaaa
     2161 accgtccatt ttgggcatca tgatgtcggt aatgattaaa tcgggccatt ccattttacc
     2221 catgcgcaaa ccttcctcgc cgtcgttggc gcatactacc cgataatcgg cggcaaggat
     2281 acggcagata aaatggcgca attcctcgtt gtcttctacc acaaggattt tggagcgttc
     2341 ttcttcggct ttgtcctcct gatgcgattc tgccggttgt tcctgctgga tgttttcact
     2401 aaggttgccg gcgtaaaatt caacattctt gtcgttttcg aaatgttttt tccctccgcg
     2461 gaaagttacg ctgaaggtgc tgccttttcc cggttggcta tctacaccga tgctggcgtg
     2521 atggagatcg acgaattgct ttaccagcga aagtccgatg cctgacgagg gcctgaacaa
     2581 attgtcttgt attacggttt cgaaacggct gaacaattgc tccacgtctt cccgtttgat
     2641 gcccttacct tggtcggtga tcgatatcac taccgaaggt tcaatatccc cattaaagct
     2701 gttgttcagc tttgaaacat ttatgcttat tgcacctccg tcgttggaat atttgaatgc
     2761 gttggataga agattgaaaa atattttttc catcttatcc ctgtctgccc atattaccgg
     2821 caaatcgttt ggacaattta aattgaaaag gatgcgatga tgattggcca attcagcaaa
     2881 attttccata atacgggaca agaggacttt tacatccaat ggctcgatca ataaagttat
     2941 tttgttgtgc tgaagtttgc ggaaatccag tatttgattc accaggttca gcaatctgcg
     3001 tacattcttc tggactactt cgagatagta acgcgattgt tcggacaact gcttatcctc
     3061 caaaacttct gccacggggc cgtctatcag ggtgagcgga gtccgcagtt cgtggctgat
     3121 gtctgtaaaa aaacgaagtt tggtatcggt gctttcctgt tcaaggatca agcggtggcg
     3181 cagccggtac atacgctgga agtagtaaac aaccaatgca ataaatagta agacggccga
     3241 aattaggata agacggcccg gcagcgtctc atgaaaaagc ggcctaacgt aaatgagcat
     3301 agtttgttca ttgtcggtcc aaatgccgtc ggcattggtc gattttacgt ggaataaact
     3361 gttgccgggc ggtaaattga tgtaagtaac ggtattctcg gttgtgatat tccagtcttt
     3421 gtccaccccg tccaacttgt atgcgtattt gaggtgtagt ttatcgtggt aatccagggc
     3481 ggcaaaacgt atgcttgccg ctctggcctg atgcttggat ttaaggtctt gtgatttgct
     3541 gctgacaatc aaagggggag tataaatgtc ttttaaaacc tgcgaagaac gtatggtaac
     3601 gataccgttc atggtaccga aaatcagagt gctgccctct gtaaggggat ggccttcgga
     3661 aaatatcacc tcggaaggaa aggaagtcct gtcgtaaata gtaataattc cggggctttg
     3721 caggccggaa ttatcggaaa cttcctggcg ttgtagtcgc gaaagcgccc ggccgccggt
     3781 aatccacaag tcgttttgcg gatcttctat tattgagtag gcgacatcgc tggcaaggcc
     3841 gtcttctttg gttatcgatt caaaacggat cctgtccgag agcaggtcat cggttccgga
     3901 gagtatacgg catattcctc cgctgctggt ggtcagataa atggagctgt cccttgtaac
     3961 caaaatgctc atgacatcgt tgtcgggcag gctgttgata tccagagacc gtcttgtatt
     4021 gcgaaacatg cgaatatttt ccggatgatc aaacaaattg ttaaacgaga acaatccgtt
     4081 tctggcccct atcaacataa gtcccggcct gatttcgcaa atgaaacgga cggtttcggg
     4141 caattcacta tcgtagccca gatgattgtt ttggtgatgg aaaacacctt gcttgaacag
     4201 attgatacct cctccaaaag tgcctatcca cagatgtccc cggctgtctt ccacaatgct
     4261 gtacacatcg ttgtgactca agctcttggg gtcgttgtcg tcgtgttggt agcggctgag
     4321 cttgtaattg tccgagccat cagtgaattc cagacaatac aagccgcccg gtttgctgcc
     4381 tatccaaata cgttgatttg aatcttcata aaggcaatac gctgtaccct caattcgaat
     4441 ctcatgtaca agttgattgt cccgaaagca aagcaccttg ttctctctca gaccgaacca
     4501 gtagcggtcc ttggaatctt tgagcatgga gcgcacttct tctccgggaa aagagttgat
     4561 tacctcgaaa ggctggttgt gcaaagtaag catatctatg tgcctgttgg tccggtacca
     4621 cagattgcct tgtttatcta tagcgactcc ttgcgtatta gttatcatcc cttccgggct
     4681 gatgggcctt tcaaatcgcc cggtaccttc gttgtaatac aaaatgtcgt ttgtgtcgta
     4741 gaaaatggac catatacgtc cgctcccgtc ttcgtaaaaa ctcacgcggg gatcggaaaa
     4801 gttatggtcc gccggaagtt ctactatttg gcattcagct gtttcaccgg aatcagattt
     4861 tgtgttaatt acaatgatct ctttaccggt agtaaggccc cagacgcagc cccggctgtc
     4921 gacaaaaaaa gtttgcagta tttctttgct cagacaaagc gtttgcctgt cggcgatcga
     4981 tgtgagatag aaacccgccc cggtagccgt atagattaaa ctgtctttaa aatgctgctt
     5041 atagacgtaa ccgttgaatg ggataaaatc caaataggta atattggcgt tttgatagga
     5101 atagattcct atttgtctgc catcggcaga agtaagccat aattcgtcct cggttacggc
     5161 aggaaagcga aaaggtattt tgcttacaag ctgatccgtg tcgtagcggt aggtgccgct
     5221 tttggttaat atccattcaa aaccctgaga atctaaaaac accagaaaaa cgtcctttga
     5281 agctgaagct ttgattgcta tctcccggca cttgttcaga ggatcggaat ccacgattcg
     5341 aaagcattct cccatctcgc cgatcatata gctcactccg ttgccgaggg gaaaaatgct
     5401 ctcaatagtt acatttctga ggtatttttg ttcaaagact gtttgcagat tggtaaattc
     5461 ttctttttcg acatcgaaca aatgaatttg gttgcctgct tcgcaccaaa agttaccgct
     5521 gctgttttcc cataaatagt tgatacggtt ggtattcagc agagattttt cgccgggacg
     5581 cgatttgtat ttggtccatt gataaccgtc gtatttgttt agtccgttga acgaactgac
     5641 ccaaatcaat ccgcgacgat cttgcaacaa ataagtgatg ctaccttgca aaaactcatc
     5701 atcgctgctg tagtgattgt aggttacaaa aggttttacc cgggaattag ccgataaaga
     5761 aaactctgcc ggggtcaaaa agaaagcaat tatgtatgca aaaagtgact tccgcaaaga
     5821 gcttgttgct ttcatgggat atagattttg ccggacaact gccggctcga tgttaaggtc
     5881 tgaggactgc caacaaaaaa aggaagaaaa gccaaacgtt tttcctcctc aacacattat
     5941 cagtgttgtg tttactccac tctcttgtta atgaagattt gtgtgattaa tccaagcaca
     6001 aacaggcctg cagccactaa aagccaggta ttggtgagaa tgcctttgaa ttgggtggcg
     6061 gcaagtacaa cagtgcctgc gagaacaata ataatgccga ggttcttgag caaaaggatt
     6121 aatatctttt tcatcgtaat ctaattgaat gaattgttct tgctactaat gtgcgcaaag
     6181 ttacactttt tattcaaata tcgattatag ttcggctttt tttacatcct gatttttttt
     6241 aaaacagatt ttgtcagaaa aaataatctt actacctttg cgccgctctt tttgtgaaaa
     6301 cactattcca gcaaaagagt tagtataaaa acaattataa attttcttat gaacaattac
     6361 gaaaccgttt tcattttaac tcccgttttg tctgagcctc agatgaagga agcggtagaa
     6421 aaatttaaaa accttctcgt tgcagagggt gctgagattg tgaatgaaga aaactgggga
     6481 ctgcgcaaac tggaatatcc cattgataaa aagtccaccg gtttttatca gatgttcgaa
     6541 tttaaggcca atccggaagt ggtggctaaa ttggaggtca atttccggcg tgacgaatca
     6601 gtccttcgct tcctaatatt gaagcaggat aaattttttg ctcaatacgc agaaaaaaga
     6661 agaagtttga aacaagctaa aaaggagaat taaactatgg cacagaatag ctcagaaatc
     6721 cgttatctgg ttcctcccac ggtggacacc aagaaaaaga aatattgccg tttcaaaaag
     6781 agcggtatca aatttataga ctacaaggat cccgaattcc ttaaaaagtt tctcaacgaa
     6841 cagggaaaga tcttgccccg ccgtcttacc ggcacttcgc tgaaatatca gcgcaaagta
     6901 gcccaggctg taaaacgtgc ccgccacctg gcgctcttgc cttacgtaac cgacttgatg
     6961 aagtaaaagg aggaatcgac atggaagtta ttttaaaaga agatgtaatc aatctcggct
     7021 acaaagacga tattgttaag gtaaaagacg gatatggacg taattacctc attccaaacg
     7081 gtttggctgt aatggctacg gagtcggcca aaaaagtgct ggccgaagac cttaagcaac
     7141 gcgcccacaa attggaaaaa atgaaagcgg atgctcagga tatggtttcc aaattcgagg
     7201 gcttgcacct gagcataggt gccaaagtga gcagcacggg aaaaattttt ggttcggtgg
     7261 gcccgattca aatagccgag gcgctcgaac aacaaggcct ggaggtagac cgcaagagca
     7321 ttttgctcaa ggaggcagtt aaggaagttg gagaatacaa agccactgtc aagctccacc
     7381 gcgaagttca ggtggaaatc agctttgagg tagtcgctga gtaattctgc aaaatataca
     7441 gacaggaggg cgtctcaatt tttttgagac accctctttt tattattatg caactatttc
     7501 cgtttagcga ctgtttccgt tctgcaactg cttccgttct gcaactgtcc cgaaaacctc
     7561 gcctcttaat ctcgatctct ttcgtcgctt taagtaatat cgaagaaata ccccacgttc
     7621 atagcataag tgtaaaacca atcccgctta tcctgtttgt ttatgaccaa tcaagcaaca
     7681 acaccatctc ccacttattt gttttatacc ctgggctgca aattgaattt tgccgaaagc
     7741 gcgggcttgg gtaaaaaaat gcaacaagtg ggttatcagc cggtaaaagc cggacgggag
     7801 ccggatatct gcgttataaa cacctgtgcg gtaaccggaa cggccgaaca aaaatgccgg
     7861 cagaatattc acaggctcat tcgcaaatac ccaaaagcct ttgtagtggt caccggctgc
     7921 tatgctcagc tcagagcggc cgaactcgca gctattcctg gtgtggatct ggtgctgggc
     7981 acagatgaaa agatggatat tttgacatat cttaagagcg ggaaactcga aaaggccgag
     8041 gtgttttcgg gccccaactc ggggattcat cgtttttttc cggcctgcgc ccgaggagac
     8101 cgcacccgtt attttctgaa agtacaggac ggttgcgatt atttttgtac ctattgcacc
     8161 attccttttg ccagaggccg aagtcgcaat atcggcatcg aagaagtcgt tcggcaggcc
     8221 ctcgaagtgg ctgccgaagg cggtcgtgaa atcgttttga ccggtgtaaa cataggagat
     8281 ttcggtaaaa gcaacggcga aaaactcata gacttgctgc gggctctgga tggagtcgag
     8341 ggcattgaac gtttccggat ttcttcgctg gaacccgatc tgctcagcga cgagatcatt
     8401 gagtttgtgg ctgcctccgc ccgctttgtg cctcattttc atttgcccct gcaggcaggc
     8461 agcaatgaga tgcttcgtct gatgcaccgg cgatacgaca gggaacattt tgcttccaga
     8521 gtagctaaaa ttcgtcaaag tatgcccggg gctttcattg gtgtggatgt aatcgtgggc
     8581 atgaggggcg aaacacaaga acgtttcgaa gagagcagaa gctttctcga aagcctcgat
     8641 attagccaac ttcacgtatt cagctactcg gaacggcccg gaacgcaagc tttaaagata
     8701 cagccgaagg tgagtagctt tgagctaaaa aggcgcagcg agatactaca tagtctatcc
     8761 gaaacaaaaa gactgaaatt ttatggtgag cgccgcggcg agcaggcaat cgtattgttt
     8821 gaaaaagcca aaaagaacaa ccttattcat ggttttaccg aaaattacgt gcgcgtagaa
     8881 gccccctggc gcggtgattg ggaaaatcgc agcattcctt tgactctcgg tgatttcaat
     8941 gcagatcgct cggctctgca ggctgtaccg attccgtgag ggctgtcttg atttcctgat
     9001 cggaaaaact tttaccttaa aaaaatccac tacctttgtg attatagtat aatcacctgc
     9061 tatgggctat gtgttcttat acggattttg tcgcttgctg gctttcttgc cgctaaacat
     9121 gctttatttg ctttcggata tgattttgta tcccctggtt tattacctgg cagggtaccg
     9181 aaaaaaggtg gtccgtacca atttggctgc cagtttcccg gacaaaagcg ataaagaacg
     9241 gcgcagcata gaaaaagatt tttacaggca tttttgttcg gtaatagtag aaaccatcta
     9301 cctggcagga atttctaccg aagaagccaa aaggcgtact tcttttatta acggcgaatt
     9361 gcttgaaaaa tacctagacc ggggacaatc cgtattggtt tacctgggtc attacggcaa
     9421 ttgggaatat cagaccttcc taacacttca ttaccccggt atccatctga tcaatgttta
     9481 cagacctttg accagcaagg ccttcgatcg cctgatgttt aaaatcagag accgtttcga
     9541 tgccgaactc ttggaaaaac aggctgtttt acggaaaatg gtttcgctca ggcagcaagg
     9601 gcaggctgcc gtatttggaa tgatagcaga ccaaagccct tccaaaacca atttacacta
     9661 ttggaccagg tttttgaatc aggacacttc tgtgctcacg ggggtggaac gcctggccaa
     9721 aagcagtggc gctgcagtgg tttatggtga tgtcagggtg ataaaacgag gttattacag
     9781 catggagttt aagctaataa cagaccgggc ccgggagaca gccgacaacg aaattacgga
     9841 aaagtacagc cgtctgatgg aagaaagcat tttgcgtaat cctgcctttt ggttatggac
     9901 ccacaggcgc tggaaacaca aacggaatca ggccgaaaaa ccggcagtcg gacagattcg
     9961 tgagtccgag gaaccggcag caggctcaac cggcattagg tataaatcta acgcaaagtg
    10021 gggcccggaa atataagcaa tatggagacc aacaacacta aagccgtcac caacgaaaac
    10081 gccaacggcg gcaccggaac taaaactaag accggggtca atatcaaaac cacggtagtt
    10141 attctcaatt ggaacggcaa agccttgatg gaacagtatc tgccggtgct gatagaacat
    10201 acctcttatc ccgatgtgtc tttgatggtg gcagacaacg cctcgacgga tgcttctgta
    10261 gaatggctgc aaaccaccta tcccggtatt cagcttcttt gtttagatcg gaattacggt
    10321 tttgccgaag gctataaccg ggcccttgcc ggcctcgaga gcgaatactt ggttctgctt
    10381 aattccgatg caaaagttac cccaaactgg ttgcccccga tgatagatta cctggatcaa
    10441 catcccggtg tagctgcttg tcagcccaaa atactaaacc tgagacagcc cggttatttc
    10501 gaacatgcag gagcctgcgg aggttttatc gacactttgg gctatccttt ttgccgggga
    10561 cgtatccagg attacctgga gcccgatcag gggcaatacg acagcatttg cgatgtgttc
    10621 tggaccagcg gagcctgcct gtgtatacgg cgtgaacact tttttgcagc cggatgcctc
    10681 gacggacgtt tctttgccca tatggaagaa a
//
LOCUS       DAOSGL010000042        10682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5215_length_10682_cov_3.822339, whole genome shotgun sequence.
ACCESSION   DAOSGL010000042 DAOSGL010000000
VERSION     DAOSGL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_10682_cov_3.822339"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PKY68_02430"
     CDS             <1..877
                     /locus_tag="PKY68_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOH23929.1"
                     /translation="SAGVLGEIVLNYRLPEVLTAIVAGSALSVSGLMMQTLFRNPLAG
                     PDVLGVNAGASLGVAALTMFASTLSGFALGLGSWGLILAAITGALLVLLLVLFVSARI
                     PDLVSLLIVGMMFGNISGAIVSMLQSLSNPDLLKIFVVWTFGSLSGVTWSMMPVLVLL
                     VLTGLIMAYMMQKNMNAMLLGERYARGLGVRLNSMRFTMILSTALLAGSITAFAGPIA
                     FIGITTPHIARGLFKTWDHTILLPAVMLCGSILLIACDLVAQSAIFNNSLPINAVTAL
                     VGAPLIIWIILKNNR"
     gene            complement(855..1553)
                     /locus_tag="PKY68_02435"
     CDS             complement(855..1553)
                     /locus_tag="PKY68_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOH23930.1"
                     /translation="MGLVFTGDLDTYRYGVWKMDAEQQDVGNVPPDFLVPEFKSAHRR
                     TEFISVRRLALAMGVDPSDIDYTVSGKPLLRSLPETHISISHSRRYAAFMVSKQAYTG
                     IDIEEFSERILRVRSRFMSEEEENKLRQLFAGQTYPAEAELIALYLHWCSKEAMYKAL
                     DIPSLDFKQDLKVDICGINGGDNSATGDNFLLGNFLKKIQGRFKACYTPKQIRFNIDF
                     FIREDFVLTCCSSE"
     gene            complement(1568..2050)
                     /gene="ssb"
                     /locus_tag="PKY68_02440"
     CDS             complement(1568..2050)
                     /gene="ssb"
                     /locus_tag="PKY68_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH23931.1"
                     /translation="MSINKIILVGNVGRDPDVRYLDNGVSVARFSLATTERGYTLANG
                     TVVPERAEWHNIVAWRGLAELAEKYIRKGSQLFVEGKITYRTWEDKNGQTRYTTEIVA
                     ENIELLGRRQQSQGEGGSSASADSSPTDPAEEKSPQAPAPPVDSGQADTADDADDLPF
                     "
     gene            complement(2168..3319)
                     /gene="mutY"
                     /locus_tag="PKY68_02445"
     CDS             complement(2168..3319)
                     /gene="mutY"
                     /locus_tag="PKY68_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOH23932.1"
                     /translation="MNKGENEKRISLTLAQWYEQNKRDLPWRNTHDPYKIWLSEIILQ
                     QTRVDQGLSYYLRFISSFPDVFSLARAQEDEVLKLWQGLGYYSRARNMHKTASEIVSV
                     YKGVFPKDYHTLLRLKGIGPYTAAAIASFAYNQAYPVVDGNVIRVLARIFNIDLPIHS
                     SRANTLFNELALALLDKVNPARHNQAIMEFGALQCKPGQPDCSVCPLNELCLAYSLGV
                     VDKRPVRKPTPIRKKRFFNYFCLSNGRHTWLQQRKNKDIWQNLWEFPLYETTELLDWE
                     TAMAVLSKFLPVPPTELNPDLPSGRTELLNSKTVLRSVQNLNHILSHQHIQARFFTIL
                     IPESVEALPTEWSKIELNEIDKLAVSRLTEIYLENHMNELDFNSNRDIC"
     gene            3545..3823
                     /locus_tag="PKY68_02450"
     CDS             3545..3823
                     /locus_tag="PKY68_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOH23933.1"
                     /translation="MTKADIVNEIAKRTGAEKSVVLSCVETFMEVVKESLIGGENVYL
                     RGFGSFIIKRRAEKTARNISKNTTIIIPAHNIPAFKPAKSFLNEVKEK"
     gene            4095..5672
                     /locus_tag="PKY68_02455"
     CDS             4095..5672
                     /locus_tag="PKY68_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOH23934.1"
                     /translation="MVTELVVDVQAKEISYAIIEDKSLVEFQQESVNLSFSVGDIFLG
                     RVHKVMPGLNAAFVDVGYEKDAFLHYLDLGPQFNSLNAFTKQQISNKKKQLTLSKFNL
                     LPEIPKDGQITELLSPGQEIPVQIAKEPISTKGPRLTSEISIAGRFLILMPFSDRISI
                     SNKIKSPEERFRLKQLLQSITPKNFGVIVRTVAENKKVAELDSELKLLLKRWDDIVLK
                     LQNKRKAPQLIFEETSRSVVILRDILNSSFENIYVNSHEVYEEIKNYVNLIMPEKANI
                     VSEFKRKVPIFDFFGITKQIKSGFGRIVSIKSGAYLIIEHTEALHVIDVNSGNRSRTN
                     KGQEANAFEVNMAAADEIARQLRLRDLGGIIVVDFIDMLEGNNRQKVFDRLKEKMSTD
                     RAKHNILPLSKFCLMQITRQRVRPQTFVQTSETCPTCFGKGEIRPSILFTDILENKIE
                     YLIRKLKLKKCTLRVHPYVAAYIEKGGLNSLKWKWRRKYGLGINVVPSQAMAFLEYAF
                     FDGEDQEIDMKEELELK"
     gene            complement(5753..7513)
                     /locus_tag="PKY68_02460"
     CDS             complement(5753..7513)
                     /locus_tag="PKY68_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOH23935.1"
                     /translation="MEVTRLFDLLDNYLEKYPDQAVALAGKVNHQWVRYSIQDYVKEV
                     NRLSYAFLAKGVKPGCKIGIISNNCPEWNFFDMAIMQIGAVGVPIYPTISQSDYKYIL
                     KHSDVEYVFVDSKELVSKLQPLVADNPLFKEFICLQETENAISLADFRALGDANPNPK
                     EVTKRKAGIKEDDMASIIYTSGSTGMPKGVMISHKNILCQIMDFYHVPAEWSKTALSF
                     LPLCHAYERLLIYLYHFLGISIYYAENIGTIAENIKEVKPTMMTAVPRVLEKIYDKLF
                     LAGKSQKGIKKNLYYWAFELATRYKLEGNGWWYDLQHTIADKLIYSKWRQAIGGNFDL
                     IVSGGAAIQKHQSAFFNAIKIPVFEGYGLSETAPVIAVSRRGKNNRREGTVGPPLTCV
                     EVKITEENEIICRGPNVMLGYYKAPELTAEVIDKDGWFHTGDTGKFTPEGLLIITGRL
                     KHIFKTSFGKYINPFLIEEKFSKSPFIENIVVLGENRQFATALIVPDFTYLRNFCKRR
                     NMPLVDNAELVKRQDIIDIYLREVKKFNAFFAPHEQIKRFDLIADEWNQQNGILTPTL
                     KVKRNVIQEKYADRIDKLYK"
     gene            complement(7634..8113)
                     /locus_tag="PKY68_02465"
     CDS             complement(7634..8113)
                     /locus_tag="PKY68_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOH23936.1"
                     /translation="MSIEIERKFLVKASFKEFSKSNVYIVQAYLSIGPKCSIRVRICG
                     DKAYLTIKGETSASGLSRYEWERVLTVDEARDLIKLSEFEAIEKTRYFIPQGKHCYEV
                     DVFEGKNSGLIVAELELESEDETYEKPSWLGAEVTGDIRYYNANLQINSYSEWKDDV"
     gene            8183..8638
                     /locus_tag="PKY68_02470"
     CDS             8183..8638
                     /locus_tag="PKY68_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23937.1"
                     /translation="MKTKISWLILPFMVWGLSFSLAAAPATPKAFVRQFEKFVEEVEK
                     SHQEYKLEDWKLAEEKYKEFVKTHYPKFKDQFNREQLEKINYLQGKYIGFLTKSKFKQ
                     IRERAREWLKYASESSEGFLEALFGKENPKKENPAEEDKENKEGGEKGP"
     gene            complement(8635..8997)
                     /locus_tag="PKY68_02475"
     CDS             complement(8635..8997)
                     /locus_tag="PKY68_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOH23938.1"
                     /translation="MEIKGKIIKILPLQSGEGRNGQWRKQEFVLEFGDQYPKIVCFSL
                     RGNRIDQFPLELNEQVVVYFDLESREYNGRYYTDVKAWKIEKVNVLPDNEKSTTDNSS
                     DDNYPPVGEMPKSDGMPF"
     gene            complement(9043..9130)
                     /locus_tag="PKY68_02480"
     tRNA            complement(9043..9130)
                     /locus_tag="PKY68_02480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9094..9096),aa:Ser,seq:tga)
     gene            complement(9386..9934)
                     /locus_tag="PKY68_02485"
                     /pseudo
     CDS             complement(9386..9934)
                     /locus_tag="PKY68_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(10040..>10682)
                     /locus_tag="PKY68_02490"
     CDS             complement(10040..>10682)
                     /locus_tag="PKY68_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOH23939.1"
                     /translation="TTIEVGDKIIQTASASFSDFMAYYEVHLMNPKVLVGMFIGSMMT
                     YVFCGLTMNAVGRAAQKMVEEVRRQFREIKGIMTGKATPDYARCVEISTKGAQVEMLF
                     PSLLAIIVPTLTGLIFGVAGVMGLLIGGLSTGFITAIFMANSGGAWDNAKKYVEEGNF
                     GGKGSEAHKATVVGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGLTVAWSLL"
BASE COUNT         2952 a         2265 c         2316 g         3149 t
ORIGIN      
        1 atccgccgga gtacttggag aaattgtcct gaattaccgt ttacctgaag tgctgactgc
       61 tattgtggcc ggttcagcct tgtcggtatc gggtttgatg atgcagactt tgtttcgtaa
      121 tcctttagcc ggtcctgatg tgctgggagt gaacgccgga gcgagtctgg gggtggctgc
      181 attaaccatg tttgcatcta ctctgagtgg ttttgctttg ggcttgggtt cctgggggct
      241 gattctggct gctattacgg gtgccttgtt ggttttatta ctggtgcttt ttgtttctgc
      301 ccgcattccc gatttagtct cactgcttat tgtcggtatg atgttcggca atatatccgg
      361 ggccattgtc agtatgctgc aaagtctgag taatccggat ttgcttaaaa tatttgtagt
      421 ttggacattt ggcagtttaa gcggggtcac ctggtcgatg atgccggtgc tggtgttgct
      481 ggtactgaca ggtctgataa tggcttacat gatgcagaaa aatatgaatg ccatgctgct
      541 gggagaacgt tatgcccggg gcttgggcgt ccggcttaat tccatgagat ttactatgat
      601 tttgtctact gcactgttag cgggcagtat aacggctttc gcaggcccca tcgcctttat
      661 aggtattaca acacctcaca ttgcccgggg gttgttcaaa acttgggatc ataccatttt
      721 gctgccggcc gtcatgctct gcggttccat attgttgata gcctgcgacc tggttgcaca
      781 atcggccatc ttcaacaaca gtttgcccat caatgccgta actgctttgg tcggagcccc
      841 tttgattatt tggatcattc tgaagaacaa caggtaagga caaaatcctc tcgaatgaaa
      901 aaatcgatgt taaagcggat ttgcttcggg gtataacagg ccttgaagcg accttgaatt
      961 tttttaagga aatttccgag caagaaattg tctcctgttg cggaattgtc tccgccattt
     1021 attccgcaga tatcaacttt caaatcctgc ttaaagtcga gagacggaat gtctaaagct
     1081 ttgtacatag cttctttgga acaccaatgg agatacaagg caatcaattc tgcctcagcc
     1141 ggataggttt gtccggcgaa taattggcgg agtttgttct cttcttcctc gctcataaac
     1201 ctggaccgga ctcttagtat tctttccgaa aactcttcga tatctatacc tgtataggct
     1261 tgtttgctga ccataaaagc agcgtaacga cgggaatggc tgatgctgat atgtgtttcg
     1321 ggcaaactcc tcaacagagg tttgcccgaa acagtataat caatgtctga tggatcaact
     1381 cccatggcaa gagccagcct tcgtacggaa ataaattccg tccttctgtg cgcagattta
     1441 aattccggta cgagaaagtc cggtggaaca ttgccgacat cttgctgttc tgcgtccatt
     1501 ttccagacac cgtaacggta agtgtccaag tctccggtaa aaaccaggcc catgcgtata
     1561 agcttagtta aaagggaagg tcgtctgcgt cgtcagcagt atcggcttgg cctgaatcaa
     1621 ccgggggagc aggtgcctgt ggggattttt cctctgcggg atcggtcggg ctgctgtctg
     1681 ctgaagcgga gctgccgcct tctccctgac tttgctgacg gcgccccagc aattcaatat
     1741 tctcggccac tatctctgtt gtgtaacggg tctggccatt tttgtcttcc caggtgcggt
     1801 aggtaatttt gccttcgacg aaaagctgag agcccttacg aatgtatttt tcggccagtt
     1861 cggctaaacc cctccaggct acgatattgt gccattcagc acgctctgga acaactgttc
     1921 cgtttgccag tgtgtaacct ctttctgtgg tggccagcga aaacctggca acggaaacac
     1981 cattatcgag ataacggaca tcggggtctc tgcccacatt gcccacaaga atgattttgt
     2041 taatagacat aattgttaga atttaggtgt gttttacatc tgtgtttgct gaataaaatt
     2101 ttattcgagt ccaaaaataa cagtaaaatg ctggaaaaac aaatgaaaaa ttaatattaa
     2161 actaagatta acaaatatct ctattcgaat taaaatcaag ttcattcata tgattttcca
     2221 ggtagatttc agtaagtcga gatacagcca gtttatcgat ttcgtttaat tcaattttgc
     2281 tccattcggt aggtaaagcc tcaacgcttt ccggaatcag aatggtgaaa aaacgggcct
     2341 ggatatgttg atgggacaag atatggttca ggttttgcac cgatcgaagc acagtcttgc
     2401 tattcagcaa ttcagtccgg ccggaaggta aatcgggatt taactcagtt ggcgggaccg
     2461 ggagaaattt agatagaaca gccatagctg tctcccaatc gagtagttct gtagtttcgt
     2521 acaaagggaa ttcccaaaga ttttgccata tatccttgtt cttgcgttgt tgcagccagg
     2581 tgtgacggcc gttgctaagg cagaaatagt tgaaaaaacg ttttttcctg ataggagtgg
     2641 gtttgcgtac aggtctttta tcgacaacac ccagtgaata ggcaagacaa agctcattca
     2701 gaggacagac tgagcaatcg ggctgtccgg gtttacactg taaagctcca aattccatta
     2761 tggcctgatt gtgacgggcc ggattaactt tatcgagtag ggccagggca agttcattaa
     2821 acagagtatt tgcccggcta ctgtggatgg gcaggtcgat attgaagatt ctagccagca
     2881 cccggatgac attaccgtct accacgggat aggcctggtt gtaagcaaaa gaagcaatag
     2941 ctgctgccgt gtaagggcca atgcctttta accgcagtag agtatggtaa tcttttggaa
     3001 aaactccttt atatacggag actatttcgg aagcggtttt gtgcatatta cgggcccggc
     3061 tgtaatatcc caaaccttgc caaagcttca gcacttcgtc ttcctgagct ctagccagag
     3121 aaaaaacatc gggaaagctc gaaatgaatc gtagataata ggaaaggccc tgatcaacac
     3181 gagtttgttg gagtattatt tcggaaagcc atattttgta gggatcgtgg gtgttgcgcc
     3241 aaggcaaatc cctcttattt tgttcgtacc attgcgctaa agtgaggctg atacgttttt
     3301 cgttttctcc tttattcatg ctgcaaagta aagaaaaaac acgtcgatca ccgtagaaaa
     3361 ttcagcaaat aaacttatcc ggtaaagaaa gctaaagtcg actttaaaat tcttaatctt
     3421 gaagccgaaa acaaagattt tttttgaaag attttttttg tagaatataa agctatatat
     3481 ttgcaagcca aaaatatagg aaactcaaga attattcatt gtataacttt taatctttat
     3541 caaaatgaca aaagctgaca ttgttaacga aattgcaaaa agaaccggtg cagaaaaatc
     3601 tgttgttctt tcatgcgtag aaacttttat ggaagtagtt aaggaatccc ttataggtgg
     3661 tgaaaatgtt tatttgagag gctttggcag tttcattatt aagagaagag ccgaaaagac
     3721 agccagaaat atctccaaaa atacaacaat catcattcct gctcacaaca tccccgcttt
     3781 caagccggct aagtcctttt taaacgaggt taaagaaaag taatttatat ttacattaac
     3841 agatacacca atgcctagcg gaaaaaaaag aaaaagacat aaaatgtcta ctcataagcg
     3901 taaaaaaaga ttgagaaaaa acagacataa gaagaaatga ttttctgaat ctgaattaac
     3961 aaagagaaca aacttcaagc tgtattcagt cttcaagttt gttcttcttt taagtggccg
     4021 gagctaagat atattttcaa accgttccgg ttattaggtt taacacaata tattctcgta
     4081 gtaaaaacaa accagtggta acagaactag tagtagatgt tcaagccaaa gaaatttcct
     4141 atgctattat cgaagataaa agtttagtgg aatttcagca ggaaagcgtg aatctttcct
     4201 tctcggtggg ggacattttt ttaggccgtg tgcataaagt aatgcccggg ctgaatgctg
     4261 cttttgtgga tgtgggctac gagaaggacg catttttaca ttacttggat ctggggcctc
     4321 agtttaattc tctgaatgcc tttaccaaac aacaaatttc gaacaagaaa aagcagctca
     4381 ctttaagtaa gttcaattta ttacccgaaa ttcccaagga cggacagata accgaattac
     4441 taagccccgg tcaggaaata ccggttcaaa ttgccaaaga acctatttcg actaaaggac
     4501 ccaggttgac ctccgagatt tcgatagccg gacgattttt gattctgatg cctttttcag
     4561 acagaatatc catttccaat aagataaaat cgcccgaaga gagatttagg ctcaaacaat
     4621 tacttcaaag cattactccc aaaaattttg gagtgatagt tcgtactgta gcagaaaaca
     4681 aaaaggtagc cgaactggac tccgagttga aattattatt aaaacgctgg gacgatattg
     4741 ttctcaagtt gcagaacaaa agaaaggcac ctcaattgat tttcgaagaa accagccgtt
     4801 cggtggtaat attgcgggat attctgaact cttctttcga gaacatttat gtcaattcac
     4861 acgaggttta cgaagaaata aaaaattacg taaacctgat tatgcccgaa aaagcaaata
     4921 tagtcagtga attcaagcgt aaggttccca tatttgattt tttcggcatc actaagcaga
     4981 ttaaatcggg attcgggcgt attgtgtcca ttaaaagcgg tgcttatctg attattgaac
     5041 acaccgaggc tttgcatgta attgacgtga acagcggaaa ccgctccaga accaacaaag
     5101 ggcaggaggc caacgccttc gaagtaaata tggctgccgc cgatgaaatt gcccgccagc
     5161 tccgtttaag agatctgggt ggcatcatcg tggtagattt tatcgatatg ctcgaaggca
     5221 acaaccgtca aaaggtgttc gacagactta aggaaaaaat gtcgaccgac agagcaaaac
     5281 acaatatact ccctttgagc aagttttgcc tgatgcaaat tacccggcaa agagtaagac
     5341 cccaaacttt tgttcaaacc tccgagacct gtcccacttg tttcggcaaa ggagaaatca
     5401 ggccttccat tctgtttacg gatattttgg aaaacaaaat cgaatacctg attcgaaaat
     5461 tgaaattaaa aaaatgcacc ctcagagtcc atccctatgt ggcagcttat atcgaaaaag
     5521 gaggtttaaa ttctttaaaa tggaaatggc gacgaaaata cggcttaggt ataaatgtcg
     5581 taccctctca ggccatggct tttcttgaat atgctttttt tgacggtgaa gatcaggaaa
     5641 tcgatatgaa agaagaattg gagttaaagt aattccacta gccataatta aaaaaaccgg
     5701 gcagccagcc cggttttttt ttatccatca aggatctcta ttgataccaa ctttatttgt
     5761 acaatttatc tatgcggtcg gcgtattttt cctgtatcac attccgtttg actttgagag
     5821 taggggtcag aatgccgttt tgttgattcc actcgtcggc gattaaatca aatcgtttta
     5881 tttgctcatg aggagcaaaa aaggcattga atttcttgac ttccctcagg tagatgtcga
     5941 tgatatcctg gcgtttaacc aattctgcgt tgtcaaccag aggcatatta cgacgcttac
     6001 agaaattcct gaggtaagta aagtccggaa caatcaatgc agtggcaaat tgtctgttct
     6061 cgcccagcac cacaatgttt tcgatgaatg gcgacttgct gaatttttct tctatcagga
     6121 aggggttgat gtatttgccg aaggaggtct taaagatgtg tttcaatcga ccggtaatga
     6181 ttaataaacc ctcgggagta aattttcccg tgtcgcctgt gtggaaccag ccgtctttat
     6241 ctatgacttc tgcagtcaac tcaggcgctt tgtagtagcc gagcattacg ttggggccgc
     6301 ggcaaatgat ttcgttttct tcggtgattt ttacctccac acaagtgaga ggaggcccaa
     6361 cagtgccttc tctacggttg tttttgcctc tgcggctcac ggcaattacg ggagctgttt
     6421 cgctgagtcc gtaaccttcg aacactggta ttttaatggc attaaaaaag gcagattggt
     6481 gtttttggat agctgcacct cctgaaacaa ttaaatcgaa attccctccg atagcctggc
     6541 gccatttaga atagataagt ttatcggcta ttgtatgttg cagatcatac caccagccat
     6601 tcccttcgag tttgtagcgt gtggccagtt caaaggccca gtagtaaaga tttttcttta
     6661 tacccttttg agacttacct gccaggaaca gtttgtcgta tattttttcc aatactcgcg
     6721 gaacagccgt catcatggtg ggtttaactt ctttaatatt ctcggctatg gtgccgatat
     6781 tctcggcata ataaatagag attccgagaa aatggtacaa ataaatcaat aggcgttcat
     6841 aagcatgaca aagaggtaaa aagctgaggg ctgttttact ccattcggcg gggacatggt
     6901 aaaaatccat gatttggcaa aggatattct tatgactgat cataactcct ttgggcatgc
     6961 cggtgctgcc ggaagtatag ataatactgg ccatgtcgtc ttctttgatg ccggcttttc
     7021 tcttagtcac ctctttggga ttgggattgg catcacctaa ggcgcgaaaa tcagccaggc
     7081 ttatagcatt ttcagtttct tgcagacata taaactcttt aaataaaggg ttgtctgcca
     7141 ctaaaggttg aagtttgctt accaattctt tactatccac aaagacatat tctacatcgg
     7201 aatgctttaa tatatacttg taatcgcttt gactaatggt gggataaatg ggtacaccga
     7261 cagctccaat ctgcatgatg gccatatcaa agaagttcca ttccggacag ttgttactga
     7321 taataccgat tttacagccg ggcttcactc ctttagccag aaaagcataa ctcaagcgat
     7381 taacttcttt aacataatcc tgaatactgt aacgaaccca ttggtggttt actttgccgg
     7441 ccaaggctac tgcctgatcg gggtattttt ccagataatt gtcgagcaga tcaaatagac
     7501 gagtaacttc catacatttc ctctttttac aataaactta ttttgaaata ttcaaaccgc
     7561 aaaaatagac ataaaaatcg tgtcagaata aaaaagtggt caaaaactga atagagggac
     7621 gattgccccc ggattaaaca tcatctttcc attccgaata agaattgatt tgcagattcg
     7681 cgttataata ccgaatgtct cctgtgactt cggctcccag ccaggagggt ttttcataag
     7741 tttcgtcctc gctctccagt tccagttccg ccacaatcaa tccgctgttt ttaccttcaa
     7801 acacatctac ttcgtaacaa tgttttccct gaggaataaa gtaacgcgtt ttttcaatag
     7861 cttcaaattc agacagtttg attaaatcgc gggcttcgtc cactgtcagc acgcgttccc
     7921 attcgtaacg gctcaggccc gatgcggaag tttcgccttt gatggttaaa taggctttat
     7981 cgccgcaaat cctgacgcgt atactgcatt tcggtccgat actcaagtaa gcctgtacga
     8041 tataaacatt gcttttggaa aattctttaa aagaggcttt tactaaaaat tttcgctcga
     8101 tttcgatgga cataaatctt gtttttgccg ttaaaattag aaattttact ttaccttcgt
     8161 tctgtttttc agacggatta ttatgaaaac aaaaatcagt tggcttattt tgccgtttat
     8221 ggtctggggc ttaagctttt ccctggctgc tgctcctgcc acgcctaaag cttttgtaag
     8281 acagtttgaa aagtttgtcg aagaagtgga aaaatcacat caggaataca aactggaaga
     8341 ttggaaattg gccgaagaaa aatacaagga gtttgttaaa acccattatc ccaaattcaa
     8401 ggatcaattc aaccgggaac aactggaaaa aattaattac ttgcagggga aatacatcgg
     8461 atttcttacc aaaagcaagt ttaagcaaat ccgagaacgg gccagggaat ggctgaaata
     8521 cgcttcggaa tcttcagaag gtttcctgga agccctgttc ggcaaagaaa atccaaagaa
     8581 agaaaatccg gccgaggagg ataaggaaaa caaagaagga ggagagaaag gaccttaaaa
     8641 gggcataccg tcgctcttcg gcatttctcc cacaggagga tagttatcgt cgctgctgtt
     8701 gtctgtcgtt gatttttcat tatccggaag aacattgact ttttctatct tccatgcttt
     8761 gacatcggta taatatctgc cgttgtattc ccggctttcc aaatcgaaat aaacaactac
     8821 ctgctcgttc aattcgaggg gaaattggtc tatgcggttt cccctgaggc taaagcaaac
     8881 tattttagga tactgatcgc cgaattccaa cacaaattcc tgctttctcc attgaccatt
     8941 gcgcccttcg ccactttgga gcggtaagat cttgataatt tttcctttga tttccattcc
     9001 agctacagat ttaaaatcag taaatcggct gattaattaa tttggcggag gaagagggat
     9061 tcgaaccccc ggaacctctc ggttcaacgg ttttcaagac cgccgcaatc gaccactctg
     9121 ccatccctcc atctatcgga aacgacttcc ggtagattgt tattaggctt ttttcgcctc
     9181 tttattcgtt ggcgggcttt tcgtcagcag tttgttgagc ttcttcagaa ccggaagcag
     9241 cctgagcggg ctcttctaca gcttcagctt tttcttcggt agctttagct tctgcttctg
     9301 caacttcagc cttcgcttcg gtagctttag cttccacaag ctcggctttt gcttctgtgg
     9361 tatccgtctc aactttagca acctttgctt cagcagcctc ttccgcagct tttgcttccg
     9421 tttctgcagc ctcggcagcc tcagctgcgg cagcgtccac ttgtgcctgt aacaagtctg
     9481 ctttcttttt ggctaaagca tcggcttttg ctttataaac ttctttttct tcttcgagac
     9541 gttgtttgaa taaagccttt tcggcttgac tcagactgga gattttggca cttagagctg
     9601 cctgtttttc ttttttccag gcttcgaatt tctgttcagc cacttcggcg ctaaaagctc
     9661 ctttctttac cccgccattg aggtgttttt tcaacaatac tccttctcta gagagaatgt
     9721 tacgaacggt gtcggtgggt tgagcaccgg tttccagcca atacaaagca cgatcgaagt
     9781 tcaaatctat tgtagcagga tttgtgttcg ggttgtacga acctaatctc tcaataaact
     9841 taccatctcg tggtgccctg ctgtctgcgg cgacaatgtg gtaaaacgcg tacccttttc
     9901 gcccatgtct ttgcaatctg atttttgttg ccatttggtt tgaaatgaat tataagtttt
     9961 gcattagcgg ccgcaaaggt aggcaaaatt gtttatttta atgcaaaaca gtccatattt
    10021 tttagcctta acaaaggaac tacaacaaac tccaggcaac tgttaaaccg gccatcacga
    10081 tggaaaccat actcattagt ttgataagaa tgttcaaact gggtcctgag gtgtctttga
    10141 aaggatcgcc gacggtatcg ccaaccactg tggctttgtg ggcttcggaa cctttgcctc
    10201 cgaaatttcc ttcttcgacg tattttttgg cgttgtccca ggcacccccg gaattagcca
    10261 taaaaatagc cgtaataaaa ccggtactca gacctccgat caataaaccc ataactccgg
    10321 cgactccaaa gatcaatccg gttaatgtgg gaacaataat ggcaagcaag gaggggaaaa
    10381 gcatttcaac ctgggcgcct ttagtggata tttccacgca gcgggcgtaa tcgggagtgg
    10441 ctttacctgt catgatacct ttaatttcac ggaattgacg cctgacctct tcgaccattt
    10501 tttgagcggc cctgcctacg gcattcatgg ttaagccaca gaaaacataa gtcatcatgg
    10561 aaccgatgaa cattcccacc agcactttgg ggttcattaa atgtacttcg taataagcca
    10621 taaagtcgct aaagctggca ctagctgtct gaatgatttt gtctcctact tctatggtgg
    10681 ta
//
LOCUS       DAOSGL010000043        10579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5283_length_10579_cov_4.982896, whole genome shotgun sequence.
ACCESSION   DAOSGL010000043 DAOSGL010000000
VERSION     DAOSGL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_10579_cov_4.982896"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PKY68_02495"
     CDS             complement(<1..1153)
                     /locus_tag="PKY68_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23940.1"
                     /translation="MKKIFLSLSLLFSTFVLFAQTPAFPTAEGYGMWATGGRGGQVVE
                     VTTLEDNPSNPVVGSLRWAMKQHSGQPITIVFRVSGIIDLKGNDLRMKRNNVTIAGQT
                     APGDGICIKGGCVNLGGSRNLIIRHIRSRVGVLNDTVYNPEVAPTSADFIPGASLNIE
                     NGGNFIVDHCSFAWSAEENVGFYDNDNTTVQWCIFAEGLYDAGHGKGARSYGAVLGGK
                     TASYHHNLLAHNYNRSPRFGATTKNDVVMLLDFVNNVNYNFGKKNACYGGDNRQGSAG
                     LFQLNFVNNYYKQGPAYPNNRTAVFIGASYSTETGRSYGKWHLSGNYIEGSTNAALNA
                     DNYRGLDISAYTEAVPSITMATMKSDHIPVPYPVETESAHEAYSSVLRGV"
     gene            complement(1239..4076)
                     /locus_tag="PKY68_02500"
     CDS             complement(1239..4076)
                     /locus_tag="PKY68_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HOH23941.1"
                     /translation="MKKICLLFSFCALFIYAGAQSPVLKIDVNVDEARKESEVNEPGY
                     TPWKIGKGIMSTSATFEGVQFTLSCVETTGGQLRGSWAKVMVQNPYYARLTCDGANID
                     YGSQTIEAGGKLLLEIAGLPVGTHTIQTMHNSWEDPARKAPSPMNVYLDSVLIHDSVS
                     VTSRALVASEATILLTVLQVTAEDQVVRMLFEAVTSFTPPEGVNPQYNVWLNAIELNT
                     VEVSKLAKNPCPGDADMHVDADSGSLILSWSPASAKVEKHYLFLGTDKELIENADTTD
                     AALLAGIKACDDTTFLLADPYSMNTYYWRVDELDQDGILSKGNVWSFRPRQLAFRGAE
                     GYGRFATGGRGGKVVYVTNLNASGPGSFHEAVTDKSGPKTVLFAVSGRIELGGRIFMN
                     DYLTLAGQTAPGKGICLSRAPVGSGHEAITRFIRVRLGGGETADGMGMAGSNFSIIDH
                     CSISWAIDEVFSSRNAKNITLQRSMVTEALNVAGHKNYPEGKAHGFAGTVSGDIGTIH
                     HNLLAHNNGRNWSLGGGLDGAGYYAGRMDIFNNVVYNWDGRTTDGGVHEANFVNNYYK
                     RGPAGGTTTIFTAQLEGLGKGSQSYYYAGNVLENNNGSFLCDGTDNNCGRALQVASTQ
                     VVDWTVFVDEPFFPSFATVETAKDAYKSTLSDVGCTMPVFDAQDQRVVRETLNGTYTY
                     VGSKTGKKGLIDNEADAGGFEDYGNEVIDLDVFDTDRDGLPNWWEELHGSNPYSAQGD
                     FSDANADPDMDGYTALEDYLEWMSVPRYYLPKNKSIEIDLAPFAIAYTKNPQFSLATA
                     TDLSLNFSGSTVNVAPQGDYTGIRYFDFTVTDADNSSMTRRIGVYVHEDTASALSATQ
                     HKEMEFKVYPSTFSSELNIETQAGESKEINLILRNLNGQEMLREHYSIHAGAVRLQIK
                     CPAELPAQMYLLELNDAQTGMKYDVKRVFKK"
     gene            complement(4136..6304)
                     /locus_tag="PKY68_02505"
     CDS             complement(4136..6304)
                     /locus_tag="PKY68_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23942.1"
                     /translation="MKFYRTLTLISGLFFITTGLFANNGLIGWATCNTLGQNGTTGGG
                     RGQIVRVNTKAALEQYAKASGDYIILVEGSFTGTGVLQVSSNKTIVGTGNGATFDGFG
                     IDVKDKQNIILRNLTIKNAKPDAIAMRNTHHVWVDHCDLSNSDDGLFDFTIGSDYLTV
                     SWTLFHDHDKVALANSGTQHFEDVGKNKVCYHHNWFNNTVQRNPRIGYGMGHVFNNYY
                     SNVSSYCVGYHTGASVLIENNYFYKSKSPLNQMYTNVSTAANYADAEERGNIFDSTSG
                     NKKGTGKSFDPAQYYNYAFMLDDAADVPAIVQSKAGNVYGIEYEYLPSPANGAIDVRN
                     IGELWWSGIENVQSWEVYFGTDPDSMVKTSTTGRSFRPEELLPDTEYFWKVNAIKADT
                     TIEGSLWRFHTAPAKASKPYPADGELHAKLRQANTETTTIPTVLSWIPAFEAISYKVY
                     LDTTPDFSDSSYQASPATASIAPGQLRFGVKYYWRVDAVINNNTIIEGDTWDFISDVY
                     YSQEGITECEHMVLNGRAFKEEQSGVWFKASNNWVVSGEAGPGTMSSVWGGPDAICKV
                     SIDYFDESDGNGWYGFYVNDTYVRGWAASRNNDQMVTVNIENVRILRENELRIAFYTH
                     AGELCRTDKMNILITENLEEPQQLQPVVSTPYPPNQEMSMNIYTVSGLLLKTITVRSD
                     ADGRLSDQHWMELPLPSGLYFYTVQGENIPARGAQRFFKP"
     gene            complement(6398..>10579)
                     /locus_tag="PKY68_02510"
     CDS             complement(6398..>10579)
                     /locus_tag="PKY68_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOH23943.1"
                     /translation="QAKNGLIWISTRNGLTLFDGYSFRNYKSYPGDGCAMDNSRINLI
                     RENTSGDIWCLSQDGRPYLFDVSSRTFMDVFKNTRPEISHPYIVSKFYVLEKGITWVV
                     CRDRYAFRVEDGNCKDGKGIVFYGPDKDIRGENIHWIAQDREGDEWILTNRGVSVIGK
                     KTLTSPAEFHYFLEKGEYIWLASNDGKLARYSFEQDNLEFINLRGSTTAFHFMEAMKG
                     DTIALGTDKGLVLINSCDKTRKHIPVDLIPAGNAGILSVYQDAYNDYWLFAENGVYRY
                     EQATSEIRFFQSPYVYLQHEHPNRSFAYEDPYGHLWLIPGNGNFCYFDREQQELRYHY
                     SEPENSSSLISPSVRHFLFDRQGNIWIGTNNTFGKLSFYESTYRVIPAESDEFEVRAL
                     FKDSREVLWVASKHHKLKLLDENYAKIDYRLYDANTHREVRIDFNIYSIYEDKAGNIW
                     LGSRSRGLYLLKRDEQNPYTFAVQHFIHDPADSMSLSNNSIYSIFQDSRKRIWLGTYG
                     GGLNLIREDASGKINFIHSGNRLKNYPDVYAQIVRYVTEVDNTLLIGSMHGLFSFSSA
                     FESPEEIRFSRNARNEYIAGSLSNNDVMYIYSDSGNQVYILTQNGGVNKLISGNLLSD
                     NMVFEVIGEREGLASDLTLSMTEDPSGKLWIASKQDLSRYNPSGGLINNFGSYYFKQD
                     FIFSESQVINTSKGSILFGTNKGILEINPSTMSGSNYVPPLVFTEIKLNNQAVSVPID
                     QLKDIDLKSDQRNFSIQFSALDFNNSKEIRYAYILEGLEKEWHYVNDNRSAEYIKIPK
                     GRYQFVVKSTNSDGVWVNNERKLDITVLPTFWETPWAWLLYIIFFVLLVSSIVYVLFI
                     IYRLRHEVTAEQQLSEIKLRFFTDISHELRTPLTLISSPVTDVLEHEKLSETARENLT
                     IAQHNSKRLLHLVNQILDFRKIQANKMKLMVEEAEICGFISLIMENFRFKAKEKEINY
                     LFDSNEDRIYLWIDKDKFEKIIFNLLSNAFKYTPPGKSIRVELNKTEKNVEIAIHDEG
                     IGIPAAKISSLFQRFETLFSQNTLSPSSGIGLSLVKELVELHHAKIEVLSEVGKGSSF
                     RLLFPLGSEHFANNPQVEFLLSDRQSDVNLPSEEQSQTGQEDDVQDRQSILIVEDNPE
                     LISFLHNILSRDYVVLQATNGEEGWNKTQEMLPDIIISDVVMPVMDGLDMVKKIKENN
                     NTCHIPIILLSSKASLDDRIKALERGIDDYITKPFSASHLITRVQTLLEQRKFLQDYY
                     LNKFSKNQTSDADLSSESPAIASYDEQFMKQVIDYIEQNIDNVLLVVEDLAKEFPMSR
                     SVFYKKIKSLFGLSPVDLIRTIRIKRAVQLIDSSAYSFTEIAYMCGFNDLSYFGKCFK
                     KQLGLSPSEYRLKNK"
BASE COUNT         2571 a         2446 c         2570 g         2992 t
ORIGIN      
        1 caacgcctct caaaacgctg ctataagcct catgagccga ttccgtttcg acaggatagg
       61 gcacggggat atgatccgac ttcatggtgg ccatagtgat acttggaacg gcctctgtat
      121 aagcgctgat atccaatcct ctatagttgt ctgcattcag tgccgcattg gtcgaaccct
      181 cgatgtaatt gcccgaaaga tgccatttcc cgtaacttct gcccgtttcg gtactgtaag
      241 aggcaccgat gaaaacagcc gttctgttgt tgggataggc cggcccttgt ttgtagtaat
      301 tatttacaaa attcaattga aacagtcctg cacttccctg gcggttgtcg ccgccgtagc
      361 aggcgttctt tttgccgaag ttgtaattta cgttattcac aaaatccaac aacatgacca
      421 catcgttttt ggtggtggcg ccgaaacgcg gagaacggtt gtaattatgt gccagtagat
      481 tgtggtggta gctggctgtt ttgcctccca acacggcacc ataactgcgg gctcccttgc
      541 cgtgaccggc atcgtaaagc ccttcggcaa aaatacacca ttgcaccgtg gtgttgtcgt
      601 tatcgtaaaa gccgacgttt tcttcggcag accaggcaaa ggagcagtgg tctacaataa
      661 aattaccgcc gttctcgatg ttcaggctgg ctcccggtat aaaatcggca gaggtgggag
      721 cgacttccgg gttgtaaacg gtatcgttga gtacccccac ccgggaacgg atatggcgta
      781 taattaaatt ccggctgcct cccaggttga cacaacctcc tttgatgcaa atgccgtctc
      841 ccggagcggt ttggcccgcg atggttacat tgtttctctt cattctcaaa tcgttgccct
      901 taaggtctat aatacccgag accctgaaaa cgatggttat cggctgaccc gagtgttgtt
      961 tcattgccca gcgcaggctg cccaccacag gattggaagg attgtcttcg agtgtggtta
     1021 cttctaccac ttggcctccc cgacctccgg tcgcccacat tccataacct tcggcagtgg
     1081 gaaaagccgg tgtctgagca aacaacacaa aagtcgaaaa aagcaaactg agggataaaa
     1141 agattttttt catgcttgaa ttttataaat gagatagttt ctatatcggt gattctaatg
     1201 gaactttagt ccgggctaag ttggcaagcg cagcggttct attttttaaa tactcttttc
     1261 acatcgtact tcattcctgt ttgtgcgtcg tttagttcca acagatacat ttgtgccggc
     1321 aattcggccg ggcacttgat ttggaggcgg actgcacctg catggatgga atagtgttct
     1381 ctgagcattt cctgtccgtt cagattgcgt aaaatcaggt tgatttcctt gctttcgccg
     1441 gcctgagttt cgatgttgag ttcagagctg aaagtgctgg gatagacttt gaattccatc
     1501 tctttgtgtt gagttgcaga gagggccgag gccgtgtctt catgcacgta aacaccgatg
     1561 cggcgtgtca tactgctgtt gtcggcgtcg gtaacagtaa agtcgaaata gcggatgccg
     1621 gtataatcgc cctgaggtgc cacgttaacg gtggatcccg aaaagttaag actcagatcg
     1681 gtagccgtag ccaaactgaa ttggggattc ttggtataag cgatggcaaa gggtgccaga
     1741 tcaatctcga tggatttgtt cttaggcaga taataacggg gcaccgacat ccattccagg
     1801 taatcttcca gagcggtgta gccgtccatg tcggggtcgg cattggcatc ggagaaatcg
     1861 ccttgggcag aatacgggtt gctgccgtgt agttcttccc accagttggg cagtccgtcg
     1921 cggtcggtat cgaagacatc aagatcgatg acttcgttgc cgtaatcttc gaaaccgccg
     1981 gcatcggctt cgttgtcgat caggcctttt ttgccggtct tgctgcccac ataggtatag
     2041 gtgccgttca gggtttcgcg aactactcgc tgatcctggg cgtcaaaaac aggcatagtg
     2101 caacctacat cgctaagagt gcttttgtag gcgtctttgg ctgtttcgac ggtggcaaaa
     2161 gaggggaaga agggctcgtc tacaaatacg gtccaatcta ccacctgggt gctggcaact
     2221 tgcagggccc ggccgcaatt attgtccgta ccgtcgcaca aaaagctgcc gttgttgttt
     2281 tcaagaacat tccctgcata ataataactt tgagatcctt tacccaaacc ttccaattgt
     2341 gcggtaaaga tggtggttgt acccccggcg ggacctctct tgtagtaatt attgacaaaa
     2401 ttggcctcgt gtactccacc gtcggtagta cgaccgtccc agttgtacac tacgttattg
     2461 aaaatatcca tgcgcccggc ataatagccc gcgccgtcca ggcctccgcc caaactccag
     2521 ttgcggccgt tgttgtgggc cagcaggttg tggtgaatag tgccgatgtc gccgctgacg
     2581 gtgccggcaa aaccgtgggc tttgccttcg gggtaatttt tatggcctgc cacgttcagg
     2641 gcttcggtta ccatgctgcg ttgcagggtg atattcttgg cattgcgaga gctgaacact
     2701 tcgtctatgg cccagcttat ggagcaatga tctataatgc tgaagttgct cccggccata
     2761 cccatgccgt cggccgtttc acctcctccc agtcggacac gaataaaacg ggtaatagct
     2821 tcgtgaccgg agccgacagg agcccggctc aggcaaatgc ctttgcccgg agcggtttgt
     2881 ccggccaggg taagatagtc gttcataaag atgcggccgc ccagctctat tctgcctgaa
     2941 acggcgaaca gcactgtttt ggggccactc ttgtcggtta ccgcctcgtg gaagctgccc
     3001 ggtccgctgg cgtttagatt ggtcacatac accactttac cgcctcgtcc tccggtggca
     3061 aaacgcccat agccttcggc tccgcggaag gccagttgcc tggggcggaa gctccagacg
     3121 ttacctttgg acaggatgcc gtcctgatcc agttcgtcca ccctccagta ataggtgttc
     3181 atgctgtagg ggtcggccag caaaaaggtg gtgtcgtcac aggctttgat gcctgccaac
     3241 agggcggcat cggtggtgtc ggcgttttcg atcagttctt tgtcggtacc taaaaagagg
     3301 taatgtttct ccaccttggc cgaggccggc gaccagctta atatcagaga tccgctgtcg
     3361 gcatccacat gcatgtcggc atcgccgggg caggggtttt tagccagttt gctcacctct
     3421 accgtattga gttcgatggc gttgagccat acattgtatt ggggatttac cccttctgga
     3481 ggggtaaagg atgtcacggc ttcgaacaac atacgtacca cttgatcttc ggcagtcact
     3541 tgaagcactg tcagtaagat ggtggcttcc gaagcgacca gcgcccggct ggtaacgctg
     3601 accgaatcgt gtatcagcac gctgtccagg taaacgttca tgggtgaagg ggcttttctt
     3661 gccggatctt cccatgaatt gtgcatggtc tgaatggtat gtgttcccac aggcagtccg
     3721 gcaatttcga gcaagagttt gcctccggcc tctatggttt gcgagccgta gtcgatattg
     3781 gctccgtcgc aggtaagtcg ggcgtaataa ggattttgta ccatgacttt ggcccaactg
     3841 cctctcaatt gtcccccggt cgtttccaca caggaaagag taaattgtac cccttcgaag
     3901 gtagctgatg tgctcataat acctttgcct attttccagg gggtatagcc cggttcgttc
     3961 acttcggatt ctttgcgggc ttcgtccacg ttgacatcga ttttaaggac ggggctttgt
     4021 gcgccggcat aaataaacag ggcgcaaaag ctgaataaca ggcagatctt tttcattttg
     4081 accggattaa aggggcatga tatagattta aggccgggat tgatgcggat acccgttaag
     4141 gtttaaaaaa tcgttgtgct cctctcgcgg ggatattttc cccctgtacc gtatagaaat
     4201 agagacccga aggcaagggc aattccatcc aatgttgatc ggagagcctg ccatcggcgt
     4261 cggaccgcac ggtaatggtt ttaagcaaca agcccgaaac cgtataaatg ttcatactca
     4321 tttcctgatt cggagggtat ggcgtgctca cgacgggctg caactgctgt ggctcttcca
     4381 ggttttcggt gattaggata ttcatcttgt cggtacggca gagttctccg gcatgggtat
     4441 aaaaggcaat tctcaattca ttctctctga gaatgcgtac gttttctatg ttaactgtta
     4501 ccatttggtc gttgtttctt gaggcggccc atccccttac atatgtgtcg ttcacgtaaa
     4561 aaccatacca gccgttgccg tcactctcgt cgaaataatc gatggaaacc ttacagatag
     4621 cgtccggtcc accccaaaca ctgctcatag tgcccggccc ggcttctcca ctcactaccc
     4681 aattgttgga ggctttgaac caaacccccg attgttcttc tttgaaagcc ctgccgttca
     4741 ataccatatg ctcacactcg gtgatgcctt cctgagaata atatacatcg gaaataaagt
     4801 cccaggtgtc gccttcgata atagtgttgt tgttgattac cgcatccaca cgccaataat
     4861 atttgacgcc aaacctgagt tgtccgggag caatggaagc ggtggccgga cttgcctgat
     4921 aggatgagtc ggaaaagtcg ggagtagtat caagataaac tttgtacgat attgcctcaa
     4981 aggcgggaat ccagctcaaa accgtcggaa tggtggttgt ttccgtattg gcctggcgaa
     5041 gtttggcatg aagttctccg tcggccggat agggtttgga tgctttggcg ggcgctgtat
     5101 ggaatctcca taaacttcct tcgattgtgg tatcggcctt gatggcgttc actttccaga
     5161 aatattccgt atcgggcagc aactcctccg gacgaaaaga acgtccggtc gtcgaggtct
     5221 taaccatcga gtccggatcc gttccaaagt aaacttccca ggattgaaca ttttcaatac
     5281 cggaccacca taattcacct atgtttctta catctatggc tccgtttgcc ggggagggaa
     5341 ggtattcata ctcgatgcca tagacgttcc cggctttact ttgtacaata gcaggcacgt
     5401 cggcggcatc gtccagcata aaggcatagt tgtagtattg tgcgggatca aaggattttc
     5461 ctgtgccttt tttgttgccc gaagtagaat cgaagatatt gcctctctct tcggcatcgg
     5521 catagttggc agccgtagag acgttcgtat acatttgatt caggggcgat ttacttttat
     5581 aaaaatagtt gttttcgatc aacacgctgg caccggtatg ataacccacg caataagaac
     5641 ttacgttgga ataataattg ttgaacacat gccccatgcc gtagcctatg cggggatttc
     5701 tttgaacagt gttgttgaac cagttgtggt gataacatac tttattcttg ccgacgtctt
     5761 cgaaatgctg ggtgccggaa tttgccaggg ccactttgtc gtggtcgtga aacaaagtcc
     5821 aggaaacggt caggtaatct gagccaatgg tgaaatcaaa caagccatcg tcggagttgg
     5881 ataaatcgca gtgatccacc cagacgtggt gagtattgcg catggcaatg gcgtcgggtt
     5941 ttgcattttt gattgtaaga ttgcggagaa tgatgttttg tttgtctttc acatcaatgc
     6001 cgaatccatc gaaggtggcc ccattgcctg taccgacgat ggttttattg ctgctcacct
     6061 ggagtacgcc ggtaccggta aaggagcctt cgaccaaaat aatataatcg cccgaggctt
     6121 ttgcgtattg ctcaagggct gctttggtgt ttacccgcac aatctgccct ctgccgccgc
     6181 cggtggtgcc gttttggcct aaggtgttgc aggtggccca gccaatcaaa ccgttgttgg
     6241 cgaaaaggcc ggttgtaata aaaaataaac cgctgataag tgtaagggta cgataaaatt
     6301 tcatagcaaa attcgataaa aaacggaaaa atattcaact tgcaagttta gccaatagtt
     6361 attggccgaa ttaggaaaaa ttgtttccag attggaacta tttgtttttt aacctatact
     6421 ccgaaggtga aagccccaat tgttttttga agcattttcc aaaatagctg agatcgttga
     6481 agccgcacat ataggctatt tcagtaaatg aataggcgga agagtcaatt aactgcacag
     6541 cccttttaat tcggatagta cgaatcaagt ccacgggtga aaggccaaac aagcttttga
     6601 tttttttata gaagacagag cggctcatgg gaaattcctt agcaagatct tccaccacca
     6661 acaaaacatt gtcgatgttt tgctcaatat aatcgatgac ctgtttcata aattgttcat
     6721 cgtaggaggc aatagcggga gactcagaac ttaaatccgc atcgcttgtt tgatttttgc
     6781 tgaatttgtt caggtaatag tcttgtaaaa atttacgttg ttcaagcaaa gtctgtacgc
     6841 gagttataag gtggctggcg ctgaatggct tagtgatata atcgtcgatg cctctctcga
     6901 gggctttgat tctgtcgtcc agggaggctt ttgaggataa tagtatgatg ggaatgtggc
     6961 aggtattgtt gttctctttt attttcttta ccatatccag cccatccatt acaggcatta
     7021 ctacatcact gataatgatg tccgggagca tttcctgcgt tttattccac ccttcctctc
     7081 cgttggtggc ttgcagtacc acataatctc tcgatagaat attatgcaaa aagctgatta
     7141 attcgggatt gtcttcaaca atcaatatgg attgcctgtc ttgcacatcg tcctcttgcc
     7201 ctgtttggct ttgttcttcg gacggcaagt ttacatccga ttgtcgatcg gataaaagaa
     7261 attccacctg aggattattt gcaaaatgtt cgcttcccaa ggggaataac aacctgaagc
     7321 tgctgccttt ccctacttcg ctgagaactt cgatttttgc gtgatgcaat tcgaccagtt
     7381 cctttaccaa agacaaacct atgcccgatg agggcgatag tgtattttgg ctgaacaggg
     7441 tttcgaatcg ctgaaaaagc gaagaaattt tagcggcagg aattcctata ccttcgtcgt
     7501 ggatagcgat ttctacgttt ttttctgttt tgttcagttc tacgcgtatt gactttccgg
     7561 gaggagtata tttaaaggca ttggagagca gattgaagat tattttttca aatttgtctt
     7621 tatctatcca taaatagatt ctatcctcgt tggagtcgaa caaataattg atttcttttt
     7681 ctttggcctt gaatcggaag ttctccatta tcagagaaat aaaaccacag atttcggctt
     7741 cctctaccat cagtttcatc ttattggcct gaatctttct gaaatcgagg atttgattta
     7801 ccaaatgtaa aagacgtttg ctgttgtgct gggctatagt taaattttct ctggcagtct
     7861 ccgataattt ttcgtgttca agaacatccg ttacagggct ggaaatcagg gtgagcggag
     7921 ttctgagttc atgagaaata tccgtgaaga agcgcaattt tatttcggac aattgctgtt
     7981 cggcggtaac ttcgtgtcgc aagcggtaaa taataaacaa tacatacaca atgctgctca
     8041 ccaacaacac gaaaaaaata atatagagca gccaggccca gggagtttcc caaaaagtgg
     8101 gcaaaacggt aatatccagc tttcgttcat tgtttaccca caccccgtcg ctgttggtcg
     8161 atttcaccac gaattgatag cgtcctttgg gaatcttgat gtattcggcc gaccggttgt
     8221 cgttcacata atgccattct ttttcgaggc cttcgagtat ataggcatac ctgatctctt
     8281 ttgaattgtt gaaatccaaa gcggaaaatt gtatggaaaa atttcgttga tcagacttca
     8341 gatcaatgtc tttcagttga tcaattggaa cagagacggc ttgattgttg agttttattt
     8401 cggtaaaaac caaaggaggt acataattgc ttccggacat agtggaggga ttaatttcca
     8461 atatcccttt gttggttccg aaaagaatac tgcctttgga ggtgttgatg acctgggatt
     8521 cggaaaaaat aaaatcctgt ttaaaataat agctaccgaa attgttgatc aagccgccgg
     8581 aaggattgta tctcgataaa tcctgtttgg aggcaatcca aagcttgccc gatgggtctt
     8641 cggtcatgga gagggtcaaa tcggaggcca atccttcccg ttcgccgatt acttcgaata
     8701 ccatgttgtc ggagagcaga ttgccggaga tcaatttatt cactccgccg ttttgtgtca
     8761 gtatataaac ttgatttccg gaatcggaat aaatgtacat tacgtcgtta ttgctcaggg
     8821 agccggctat atattcattg cgtgcgtttc gggaaaagcg aatctcttcg ggcgattcaa
     8881 atgctgagga gaaactgaat aaaccgtgca tactgcctat aagcagggtg ttgtctactt
     8941 cggtaacata acgtactatc tgagcgtata catcgggata gttttttaaa cggttcccgc
     9001 tgtggatgaa attgattttt cccgatgcgt cttcccggat caaattaaga ccgcctccgt
     9061 aggtgcccag ccatattcgt ttacgactgt cctgaaatat cgagtagatg ctgttgttgc
     9121 ttaagctcat actgtctgcg ggatcatgga taaaatgttg cacggcaaaa gtatacgggt
     9181 tttgctcatc ccttttcagg aggtacaaac ctcttgatct ggaccccagc cagatgttac
     9241 ccgccttatc ttcgtatatg gaatagatat taaagtcaat ccggacttct ctatgggtgt
     9301 tggcatcgta aaggcgatag tcaattttgg cataattctc gtccaatagc tttaatttat
     9361 gatgtttaga ggctacccac aggacttctc gactgtcttt gaataaagcg cgaacttcaa
     9421 attcatcgct ttcagccggg attacccggt aggtgctttc ataaaaggaa agcttgccaa
     9481 aagtgttgtt ggtcccaatc cagatatttc cttgccggtc gaacaaaaaa tgcctgacgg
     9541 aaggagaaat cagactagaa gagttctccg gttcggagta atggtagcgt aactcttgct
     9601 gctcacggtc gaaatagcaa aaattgccat tccccggaat cagccacaaa tgcccgtaag
     9661 gatcttcgta agcaaagctc ctgttcggat gttcgtgttg aagatacaca tagggcgatt
     9721 gaaaaaaacg gatttccgat gtggcctgtt cgtaacggta aacaccgttt tcggcaaaca
     9781 gccaataatc gttataagca tcctgataaa cagataaaat accggcattt ccggccggaa
     9841 ttaaatcaac cggtatgtgt ttacgtgttt tgtcgcatga gttgattaag accagtccct
     9901 tgtctgtacc cagcgcaatg gtatctccct tcatcgcttc cataaaatga aaggctgtgg
     9961 tcgaaccccg aagattgatg aattccagat tgtcctgttc gaaagaatat ctcgccagct
    10021 tcccgtcgtt ggatgccagc cagatgtatt cgcccttttc gaggaaatag tgaaattccg
    10081 cagggctggt cagtgttttt ttacctataa cgctcacacc ccggttggta agaatccatt
    10141 cgtctccctc tctgtcctgg gcaatccaat gaatgttttc tcccctgatg tctttatccg
    10201 gtccgtaaaa gactatccct tttccatctt tacagttgcc gtcttctacc ctgaaagcat
    10261 aacgatcgcg gcaaacaacc caggtaatgc ccttttccag cacataaaat ttggaaacaa
    10321 tgtatggatg gcttatttcg ggccgggtat ttttgaaaac atccataaat gtccggctgg
    10381 aaacatcgaa taaatagggt cttccgtcct ggctcaggca ccagatgtcc cccgaggtgt
    10441 tttccctgat caggttaatc cggctgttgt ccatggcaca gccgtcgccg ggataagact
    10501 tgtaatttcg gaaactatag ccgtcgaaca aggtgagccc gttccgggtg ctgatccaaa
    10561 ttaaaccgtt tttggcctg
//
LOCUS       DAOSGL010000044        10575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5289_length_10575_cov_3.938023, whole genome shotgun sequence.
ACCESSION   DAOSGL010000044 DAOSGL010000000
VERSION     DAOSGL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5289_length_10575_cov_3.938023"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2262
                     /locus_tag="PKY68_02515"
     CDS             <1..2262
                     /locus_tag="PKY68_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH23944.1"
                     /translation="QYFYEYNVNTKWLDYVRQNAYTTDNNFSLSGGGDKATYRYSLGY
                     LNETGTTVGTALNRFNTSFNIRYDFSERLMVNTEFTYTQTNRDANALSTLRSEAQRKM
                     PNKSPYYIDDFTGEALPSYFSRQNEDFQGAFNGSYHYNPVAMAEMGFNRTMQREEKMT
                     LNVQYVFPFSLMYKGYVSMNMRTSKNRKFIPQEATGVLWTSNYANQSTDAFSDRMTLQ
                     TEHKLLYNKTFRDVHAVVATVLANMSQNDSYSYTSATSGNASPNLSDPVAGSVVSSIG
                     SGFSQVRSVRAVAQGVYTYDRRYVFRTTVNREGNSAMGKSERFGTFPAFGVAWNIHEE
                     PFIRDRDNPWLTEGKLRVGLGWSGNAPSGASMYLGAYQSLGEYMSMPAIYPVRIQLDK
                     LKWETSREWNYGFDLEFFRKLGFTFDHYNKYTSDLLLGNVSIPSSTSYTSIRYFNSGE
                     LSNKGYEFRITYNAINTTDWKLSFNFNISHNENKVEKLPANMKEENYTFGNGNYAVRV
                     IEGDPIGSFYGYRYLGVYDNKEETYALDEEGRVMHDYKGRPIVMKNGSTTVFPGDARY
                     QDINYDGVINEYDIVYLGNSNPKFYGGFGFNLRYKSWSLTTFFYDRVGQKIINQACMD
                     LESMYGTNNQSTAVLSRWRSPGDAASTNIPRALYGMGYNYLGSDRFVEDASFIRLKTL
                     SLTYNVPRNFLRKYNINTLSFFCTGYDLLTWTNYTGQDPEVSIPSSATSLIRDNASTP
                     VTKRLAFGFNLNF"
     gene            2286..3872
                     /locus_tag="PKY68_02520"
     CDS             2286..3872
                     /locus_tag="PKY68_02520"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH23945.1"
                     /translation="MNRFLLTTMTLLALTLSSCNEWLNILPNNEQVTDNYWKTRQDVE
                     AVVASGYYYMRQAVPTMLVWGEVRGGALYSSNSQHSYLQNFNLTPSSGICDYASLYKV
                     IGMANSVLKYAPSVTTEDETYYESVMKSHLCEAYFQRAYCYLVLVKNFREVPLVVEAF
                     VNDDADYMTPKSSETEIIAQIKDDIEKALATGAAKDIYEDTDGYTWQTKGRATKWALY
                     ALMADACLWNHDYDEAIQYCNYILDAPEGDTSFRPRFMSDMTKWYEIFYPGNSNESIF
                     ELNWETHMSQTNNFGSWFSVGASSTLKVTDHLKAKLIAETDEVMANSIRNGWATDGRV
                     GRMIFGTFVSDAADQTKYGTANYLSVWKYMGTDIADRGNTRPAQDANFILYRVPDVML
                     TKAEALVMKGGTQSWQTALDLVNRIRQRAGLDSVNINVSETDEMVMLDAVMNEWTMEF
                     LAEGKHWYNLLRMGRYDKGDGVYKERFINEIIESNQTTKDEWIKSVLMDENAWYMPIP
                     YSEISVNENLVQNPYYKTTK"
     gene            3881..5551
                     /locus_tag="PKY68_02525"
     CDS             3881..5551
                     /locus_tag="PKY68_02525"
                     /inference="COORDINATES: protein motif:HMM:NF028443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5108 domain-containing protein"
                     /protein_id="HOH23946.1"
                     /translation="MKQTSIKIFMSLAVALLLAACQDDKLNSVYQIFDDNPASKTLEK
                     NENHSEWVKILRYSDMYNALNQATQAFTLLVPDNDAVFAFYQQRGVSGIEDLGKDYAK
                     ALILHHTMLDSIMVESMQLKNSLNNLNGDAISVAIDTLNPGQFIFGGLARTTESGVHA
                     YNALMYYVDAVLTPLVESVYDKLVEDGRYGIMKAALDATAWTQQLHTIYDTVPQEGGG
                     YVVNRRAYALLAVSDEGFKRNNIQSFNDLVAKLSAGTDYTEPDNALNEYVAYHILTNN
                     YTLADLKEMSGDTSRIWETSAKNQVFMITVDTTAGDWQFNILDSAILPTRFVEEYCDV
                     LCKNGYLHEIDAYLPVWEPKPSIVVWDLANYIEVKNTVIESAGPEEYQPAEPVSSEKK
                     IDITKCGAYDYVVSESGVGGSSYAYITYVTCKNNLRDCLKYDRVVFNLGYMGSVSMKT
                     PTLVRGKYKVELNFVYLSDHNFMRQMTDGNGGMIRMTIDGENLKSVTPYTTVPRNVAK
                     VYTATLYDEVEFEQTSTHDFKFVVMDPAASTNSRFSLQFDCITFTPIE"
     gene            5558..7282
                     /locus_tag="PKY68_02530"
     CDS             5558..7282
                     /locus_tag="PKY68_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH23947.1"
                     /translation="MKTINRICLTGCMLLCLTACDENWQRHYYQSETTITNEQLIMVS
                     QTTQEYLSGNDSRHLSDMYQFLQDNKVFNMLQKKGQLHTLLMVENDAFKAPEAERAEY
                     IAHSHVTDICLSPSNLYHGERVLMWHQKFVTVGIDSLGLLGDLTHITFNGSPVKEVVK
                     TTDGYIYVLDAMIETPISLQDYINDLPEEYSLFKDMVLASGGKLFDKNNSKPIGIDAT
                     GNTLYDTVWIYTNDFFDAKNFSLSSESLTATMLLFSNDVIAQAMAQADSTLAAWDMTR
                     DREVLMRWILEVAFYDKMYEASDLIDNDDEKFVDLKSIYDRQWRVEEQSLDLDNPIKV
                     SNGIVYEVKKLRFPNNILIYRIKDFYRNYEYCTAEEKELYFKYDNLKNIEMKSEVEAW
                     TPMPGVWPMHGNVTFSCYLTDASQGFYLDMQLIKPVYDEAGNISSVRPYLIPPGSYRF
                     AMGFFQNMGVTMNVSVIVLNGSERVKLGEASVTLASTTEFHYDRGTFLSDTWPEGYKE
                     AKNDYTHSKKNNYNTDGGAVMAELEIPDLKGDGSALPMIIKLEMPTGKDSRLAFHHWC
                     LRPTSDNY"
     gene            7294..10455
                     /locus_tag="PKY68_02535"
     CDS             7294..10455
                     /locus_tag="PKY68_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH23948.1"
                     /translation="MKQFYTIITALMLALPTLAQAVEGIVISSDGQAVRGAVLSSPGQ
                     TTVVTGEDGTFRMDLQKDAVLSVWADGYYYQQIRYKGQSSLKVVLSRQDAHKYTESKV
                     LPFRVEQNVNLPEVNTLSKKDFALGARSVEQALQGELAGLQLINKSGMTGEGAFLSHH
                     GIQTLAANASPLVVINGVPFMPSMDESQLIGGFSKSIFQAYNIYDIQDISFLSGAEAS
                     VYGSLGANGVLLIETDGASSDDMDTKISYHGKFGYNWNDRRLPMMKSSQYKSYLSDIG
                     MTYYNNMETFFANFPFLSPDERDAYNYLYRFDTDWQNELFRRGVTTDHLVRVEGGDAI
                     AKYDISLGYQHDEGALKNTQSDRFNAQINSNVIVSKKFEIGTSIGLAFLNGSYQEQGL
                     IMETNPLLAAYRRAPVLSPYKNDMAGNLLETYASYYYGNNTNTDFIVSNPLAIVNTLA
                     ADKRQYDININARVLYKPTNQLSFMALLGLYYNYDQEGIFVPGINNKDVVPQFDTYGE
                     SKNIVRQGVNETFNSFYHLNAAYNNVFDDVHGVDLRLGGQIISNSFETDMGVGRNTAN
                     DFYQTLADVQRVGRFFSGYGAKWSWLNAYFQAAYTYSDLFRVAANVSTDGASSTGVNT
                     TRMGVFPSASATVMLANFDFIKSAEAVNRLNLYADYGKTGNSRFSHTYGSYYYVSRLY
                     QGIAGIVRNNVPNTEIKWEENLKANMGIDMALWRHRVQIKLGYYNNRATDVLMAGSES
                     ALYGTGVHYVNDAAIDANGFDLSLNVSPVCTRNFKWILGGNITQLNNKVTSLGNRTHI
                     INKLSDGAAIITKVGESPYSFYGYRTDGDYPVYSTVKEAQSAYKYTENGVECSRPMRN
                     RNGVEFQAGDIKYVDQNNDGIINDLDRVLLGSATPDFYGGLFNRFQYKGFAIDLNFVY
                     SLGNEAYNAVRRITESSLDMSNQSLAVLRRWTVDGQITDMPRANYYDLVGNNDFSDRW
                     IEDASYLKLRDITFSYTFDNILLGFIQGGTLYVSGQNLWCLTPYLGNDPEFSYSYSAM
                     MQGVDYAKLAAPKTVTFGVNLKF"
BASE COUNT         2878 a         2466 c         2558 g         2673 t
ORIGIN      
        1 cagtattttt atgagtacaa tgtgaatacc aaatggttgg actatgtgcg tcagaatgct
       61 tacaccaccg acaataactt ttcgctgtcg ggaggtggcg acaaggctac ttaccgttat
      121 tccctgggtt atctgaacga aacggggacc acggtgggaa ccgcattgaa ccgtttcaac
      181 acctcgttca atatacgtta cgactttagc gagcgtctca tggtaaacac cgagtttacg
      241 tatacacaga ccaacaggga tgctaatgca ctttcgacgc ttcgttccga ggcacaacgc
      301 aaaatgccca acaaaagtcc ctattacatc gacgatttca cgggcgaagc cttgccgagc
      361 tatttcagtc gtcagaacga agacttccag ggtgcattca acggcagtta tcattataac
      421 cccgtggcaa tggcggagat gggtttcaat agaaccatgc agcgcgaaga aaaaatgact
      481 ttgaacgtgc aatatgtttt ccccttcagt ctgatgtaca aaggttatgt gtcgatgaat
      541 atgcgtactt ccaagaaccg caaattcatt ccgcaagaag ctacgggcgt actctggacc
      601 agcaactatg ccaaccagag taccgatgct ttctcagata gaatgacctt gcaaacagag
      661 cacaaattat tgtacaacaa gacttttaga gatgttcatg cggtggtggc taccgttttg
      721 gccaatatgt cgcaaaacga cagttacagt tacaccagtg ctaccagcgg caacgcctcg
      781 cccaatttga gcgacccggt agcgggaagt gtggtgagca gtatcggatc gggtttttcg
      841 caggtacgca gtgtgcgtgc agtggctcaa ggggtctata cctacgatcg ccggtatgtt
      901 ttcaggacta ccgtcaaccg cgaaggtaac tctgccatgg gtaagagcga acgctttggt
      961 actttccctg cattcggtgt ggcatggaac atccacgaag aaccttttat acgcgacagg
     1021 gacaatccct ggctcaccga gggtaaattg cgtgtcggtt taggttggag tggtaatgct
     1081 ccttcgggcg catcgatgta cctgggtgca tatcagtctt taggggaata tatgagtatg
     1141 ccggccattt atcctgtccg catccagctc gataaactca aatgggaaac ctcgcgcgag
     1201 tggaactatg gtttcgacct ggaattcttt agaaaattgg gctttacgtt cgaccattac
     1261 aataaataca ccagcgattt gttgcttgga aacgtcagca ttccttcgtc aaccagttac
     1321 accagcatta ggtactttaa tagcggtgaa ctgagcaaca agggctacga attccgtata
     1381 acttacaatg ccatcaacac gaccgactgg aaattgagct tcaactttaa catcagtcac
     1441 aacgagaaca aggtagaaaa actgcccgcc aatatgaagg aagaaaacta cacatttggc
     1501 aacggtaatt atgccgtgcg tgtgatagag ggcgacccca tcggttcgtt ctatggctac
     1561 cgttatttag gtgtgtacga caataaagaa gaaacttatg cactcgacga agaaggccga
     1621 gtgatgcacg actacaaagg taggcccatt gtaatgaaga atggctccac caccgtattt
     1681 cccggcgatg cacgctatca ggatatcaat tacgatggtg taatcaacga atacgatatc
     1741 gtatatttgg gcaattccaa ccccaaattc tatggtggtt tcggctttaa cctgaggtat
     1801 aaatcctggt cgctgacaac tttcttctac gatcgtgtcg gacaaaaaat catcaatcaa
     1861 gcttgtatgg acctggaaag catgtacggc accaacaacc agagtacggc cgtgttgagt
     1921 cgctggcgca gtccgggcga tgccgcttcg accaatatac cgcgtgcatt gtatggtatg
     1981 ggttacaatt acttaggcag cgaccgtttt gtagaagatg cctcgtttat tcgtcttaag
     2041 actttgtctt tgacatacaa tgtgccgcgc aattttctga gaaagtacaa catcaacacc
     2101 ttgagtttct tctgtacggg ttacgacttg ttaacttgga ccaactatac gggacaagac
     2161 cccgaagtca gcattccttc ttcggcgacg agtttgatca gagataacgc ctccacgcct
     2221 gtgaccaagc gtttggcatt tggttttaat ttgaattttt aaggagggta cacacaatga
     2281 aaacaatgaa tagatttctt ttgacgacga tgaccttgtt ggccctgaca ctcagttctt
     2341 gcaacgagtg gctgaatatt ctgcccaaca acgaacaagt caccgataat tactggaaaa
     2401 cccgccagga tgtagaagcg gtggtagcct ccggttatta ctatatgcgt caggcagtac
     2461 ccaccatgct ggtatggggc gaggtgcgcg gaggtgctct gtattcgagc aacagccaac
     2521 acagctatct gcaaaacttc aacctgacac cttcgtcggg catttgcgat tacgcttccc
     2581 tgtataaggt gataggtatg gccaacagtg tgttgaaata cgcgccctcg gtaacaaccg
     2641 aagacgaaac ctattacgaa agtgtgatga aatcgcattt gtgcgaagca tacttccaac
     2701 gtgcctattg ctatttggtt ttggttaaga actttcgcga ggtgcccttg gtggtcgagg
     2761 cttttgtcaa cgacgatgcc gattacatga cacccaaatc gagcgaaacc gagatcattg
     2821 cgcaaatcaa ggacgatatc gaaaaggcgc ttgcaacggg tgcagccaag gatatatacg
     2881 aagacaccga cggttacacc tggcaaacca aaggccgtgc cactaaatgg gccttgtatg
     2941 ccctgatggc cgatgcctgc ttgtggaacc acgactacga cgaagccatc caatattgca
     3001 attacattct ggatgcaccc gaaggcgaca cttcgtttcg tcctcgcttt atgtcggata
     3061 tgaccaaatg gtacgaaatt ttctatccgg gcaattccaa cgaaagtatt tttgagttga
     3121 attgggaaac tcatatgtct caaaccaaca actttggttc gtggttctcg gtaggagcct
     3181 catccacgtt gaaggttacc gatcatctca aggctaaatt gattgccgaa accgacgaag
     3241 tcatggccaa ttcgattaga aacggttggg ctaccgacgg ccgggtgggg cgtatgattt
     3301 ttggcacctt tgtgtccgat gcggctgacc agaccaaata cggaacagcc aattacctgt
     3361 cggtatggaa atacatgggc accgatattg ccgacagagg caacacgcgt cccgctcagg
     3421 atgccaactt tatactgtat cgtgtgcccg atgttatgct gaccaaggca gaagctttgg
     3481 taatgaaggg aggtacgcag tcctggcaaa cagcattgga tttggttaac cggattcgcc
     3541 agcgcgcagg tttggattcg gttaatatca atgtcagcga aaccgacgaa atggtgatgc
     3601 tcgacgctgt tatgaacgaa tggaccatgg agttcctggc cgagggtaag cattggtaca
     3661 atctgctccg gatgggacgc tacgacaagg gcgacggtgt ttacaaagaa cgcttcatca
     3721 acgaaatcat cgagagcaac cagaccacca aagacgagtg gatcaaatcg gtgttgatgg
     3781 acgaaaacgc ctggtacatg cctattccgt acagtgaaat ttcggtgaac gaaaatttgg
     3841 ttcaaaatcc gtattataaa accactaaat gacaagcctt atgaaacaga caagcataaa
     3901 aatattcatg tctctggcgg tcgcgttgtt gctggcggct tgtcaagacg acaaactgaa
     3961 cagtgtttat cagatattcg acgataatcc cgcttccaaa actttggaaa aaaacgaaaa
     4021 ccactcggaa tgggtaaaga ttttgcgtta ttccgatatg tacaatgcct tgaaccaggc
     4081 cactcaagct tttacgctgc tggttcccga caatgatgcc gtttttgcct tttatcaaca
     4141 acgcggcgtg tcgggcatcg aagatttggg caaggattac gccaaagcat tgattctgca
     4201 tcataccatg ttggattcca tcatggtgga gtcgatgcaa ttaaagaact ctttgaacaa
     4261 cctgaacggc gatgccatca gcgtggccat cgacactctg aatccggggc agtttatctt
     4321 tggcggactg gcccgtacca cagaatcggg cgtgcatgcc tacaatgcgc tgatgtatta
     4381 cgtcgatgca gtgcttaccc ctctggtgga gagtgtttac gacaaactgg tagaagacgg
     4441 ccgctatggt atcatgaaag cagctctcga cgcaaccgct tggacacaac aactccatac
     4501 catctacgac accgtgcctc aagagggtgg aggttatgtg gtaaacagac gtgcttacgc
     4561 attgctggcc gtatcggacg aaggcttcaa gagaaacaat attcaatctt tcaacgattt
     4621 ggtagccaaa ttgtccgcgg gaaccgacta cactgaaccg gataatgcac tgaacgaata
     4681 cgttgcctac cacattctga ccaacaatta cactttggca gacctcaagg aaatgtcggg
     4741 cgacacttcg cgcatttggg aaacttctgc taaaaaccag gtctttatga taacggtaga
     4801 taccacagcg ggcgattggc aattcaacat cctggatagt gctattttac ccacccgttt
     4861 tgtggaagaa tattgcgacg tcctgtgtaa gaacggttat ctgcacgaaa tagatgccta
     4921 tttgcccgtt tgggaaccca aaccttcgat tgtggtttgg gatttggcca actacatcga
     4981 ggtcaagaac acggtgattg aatctgcggg ccctgaagaa tatcaaccgg ccgaacccgt
     5041 gtcgtccgaa aagaaaatag acatcaccaa atgcggcgcc tacgattatg tggtgtcgga
     5101 atcgggtgta ggaggcagca gttacgctta cattacctat gtcacttgca aaaacaatct
     5161 gagagattgt ctcaaatacg accgcgtggt cttcaatctg ggttatatgg gttccgtttc
     5221 gatgaaaacc ccgactttgg tgcgtggaaa atataaagtg gaactgaatt tcgtttattt
     5281 gagcgaccac aactttatgc gtcagatgac agacggcaac ggcggtatga taaggatgac
     5341 catagatggc gaaaatctca agagtgtgac gccatacacc accgttccaa gaaatgttgc
     5401 caaagtgtat acagctactt tgtacgacga agtagagttt gagcagactt ccacccacga
     5461 tttcaaattt gtggtgatgg atcccgctgc cagcaccaac agcagattca gtctgcaatt
     5521 cgattgtatc accttcactc ccatagaata aaggcttatg aaaacgataa accgcatttg
     5581 tttaaccggc tgcatgcttc tttgtctgac tgcttgcgac gaaaattggc aacggcatta
     5641 ttatcagtcg gagacgacca taaccaacga gcagcttatc atggttagtc aaaccactca
     5701 ggaatacctg tcgggcaacg atagcaggca tttgagcgat atgtatcagt ttttacaaga
     5761 caacaaggtg ttcaacatgt tgcagaaaaa aggacagttg cacactttgc tgatggtaga
     5821 aaacgatgca ttcaaagctc ctgaagccga gagagcagaa tatattgccc attcgcacgt
     5881 caccgatatt tgtttgtcgc caagcaacct ttatcacggt gagcgtgtgc tgatgtggca
     5941 tcagaaattt gttaccgtcg gtatcgactc gcttggattg ttgggcgatt tgacacatat
     6001 tacttttaac ggttcgcccg taaaagaagt agtcaaaacc accgatggtt acatttatgt
     6061 gttggacgcg atgatagaaa cacccatctc tctgcaggat tatatcaacg atttgcctga
     6121 ggaatacagt ctgttcaagg acatggtgct ggcttccggc ggtaagctgt tcgataagaa
     6181 taacagtaag cctatcggta tcgatgctac cggaaacacc ttgtacgaca ccgtctggat
     6241 ttataccaac gactttttcg atgccaagaa ctttagcctg agcagcgagt cgctgacggc
     6301 tactatgctg ttgttttcca acgatgtcat cgctcaggct atggcgcaag ccgatagtac
     6361 tctggcagct tgggacatga cccgcgaccg cgaggtgctg atgcgctgga ttcttgaggt
     6421 ggctttttac gataagatgt acgaagcctc cgatctgatc gacaacgacg acgaaaagtt
     6481 tgtcgatctc aagtccattt acgatcgtca gtggagagta gaagaacaaa gcctcgatct
     6541 ggacaatccc atcaaagtgt ccaacggcat tgtttacgaa gtcaagaaat tacgctttcc
     6601 aaacaatatc ctgatttatc gtatcaaaga tttttatcgc aattatgaat attgcactgc
     6661 cgaggagaag gaactgtatt tcaagtacga caatctcaag aacatcgaaa tgaaatcgga
     6721 agtggaagca tggacgccca tgccgggtgt gtggcccatg cacggcaatg tcactttcag
     6781 ttgctatctg accgatgctt cgcaaggctt ctatctggat atgcagttga ttaaacccgt
     6841 ctacgacgaa gcgggtaata tcagcagtgt cagaccttat ttaatcccgc cgggcagcta
     6901 tcgttttgct atgggttttt tccagaatat gggtgtcact atgaatgtga gcgtgattgt
     6961 tttgaacggc agcgaacgcg tcaagttggg cgaggcttct gtgacgcttg cctccaccac
     7021 cgagtttcac tacgatcgcg gtactttctt gtcggatacc tggcccgaag gctacaaaga
     7081 agccaagaac gattatactc atagcaaaaa gaacaattac aataccgacg gcggagcagt
     7141 tatggccgag ctggaaatac ccgacctgaa aggagacggc tcggcacttc ccatgatcat
     7201 taagttggaa atgcctaccg gcaaagactc ccgcctggcg tttcaccatt ggtgtttgcg
     7261 tcctacaagt gataactatt aatattgcag ataatgaaac agttttatac aatcataacg
     7321 gcactaatgc tggccttgcc caccttggcg caagctgtgg aaggaatcgt tatttcgtcc
     7381 gatggacaag cagtacgagg cgctgtgcta tcgtcgccgg gacaaaccac agtggtgaca
     7441 ggagaagacg gaacgttccg aatggatttg caaaaagacg ctgtgctgtc ggtgtgggca
     7501 gatggttatt actaccaaca aattcgttac aagggacaat cctcactaaa agtggttctg
     7561 tcacgtcagg atgcgcataa atacaccgag tccaaagtac tgccttttcg tgtggaacag
     7621 aatgtcaatt tgccggaagt caatactttg tccaagaaag attttgcatt gggtgctcgt
     7681 tcggtggaac aagctttgca aggcgaactc gccggtctgc aactgatcaa caagagcggc
     7741 atgacgggcg aaggtgcttt tttgtcgcat cacggcattc agactctggc agccaacgcc
     7801 tcacctttgg tggtaatcaa tggtgtgccg tttatgcctt ctatggacga atcgcaactg
     7861 ataggcggct tttctaaaag tattttccag gcttacaata tctacgacat tcaggatatc
     7921 tcgttcctga gtggggcaga ggcttcggtt tacggttcgc tgggtgccaa tggcgtgttg
     7981 ctcatcgaaa ccgacggagc cagttccgac gatatggata ccaagatctc gtatcatgga
     8041 aagtttggct ataattggaa cgatcgtcgc ttgcctatga tgaaatcatc gcagtacaaa
     8101 tcttatctgt cggacatcgg tatgacctat tacaacaata tggaaacttt ctttgcgaat
     8161 ttcccgtttt tgtcgcccga cgagagagat gcttacaatt atctgtatcg cttcgacacc
     8221 gattggcaaa acgaattgtt cagacgtggc gtaaccaccg accatttggt tcgcgtagaa
     8281 ggtggtgatg ctattgccaa atacgatatt tcgctgggct atcaacacga cgaaggtgcg
     8341 ctgaaaaata cacagagcga ccgtttcaat gcgcaaatca attcgaacgt cattgtcagc
     8401 aaaaagttcg aaataggcac ctctatcggc ctggcctttt tgaacggttc gtatcaggaa
     8461 caaggcctga tcatggaaac caatcctttg ctggctgcct atcgtcgagc accggtgctt
     8521 agtccctaca aaaacgatat ggccggcaac ctgctcgaaa cctacgcatc gtattattac
     8581 ggcaacaaca ccaataccga ttttattgtc agcaaccccc ttgccattgt taacacgctg
     8641 gctgcagaca agcgtcagta cgatatcaat atcaacgccc gagttttgta taaaccgacc
     8701 aatcagttgt cgttcatggc cttgttgggg ctttattaca actacgacca ggaaggtatc
     8761 tttgtgccgg gtatcaacaa caaggatgtg gtacctcagt tcgataccta cggtgagagt
     8821 aaaaacatag tgcgtcaggg agtcaacgaa acctttaaca gtttctatca cctgaatgct
     8881 gcctacaaca atgtttttga cgatgttcat ggcgttgacc tccgtttggg aggacaaatc
     8941 atcagcaaca gtttcgaaac ggatatgggc gtgggccgca acaccgccaa cgacttttat
     9001 caaaccctgg ccgatgtgca aagggtaggc cggttcttta gcggttacgg agctaaatgg
     9061 agctggctca atgcttattt tcaagccgca tacacttaca gcgatttgtt ccgtgtagct
     9121 gcaaacgtct ccaccgacgg tgcctcttcg acgggtgtca atacgacaag aatgggtgtt
     9181 ttcccctcgg cttccgccac agtgatgctg gctaatttcg actttattaa atcggcagag
     9241 gccgtcaacc gcctgaatct gtacgcagac tacggcaaga cgggcaacag ccgtttctcc
     9301 catacttacg gcagctatta ttacgtcagc cgtttgtatc agggcattgc gggtattgtg
     9361 cgtaacaatg tgcccaacac cgaaatcaaa tgggaagaaa acctcaaagc taacatgggt
     9421 atcgatatgg ccctgtggcg tcatcgtgta caaataaaat taggctatta caacaatcgc
     9481 gccaccgatg tgttgatggc gggttcggaa tcggcacttt acggtacagg tgttcattat
     9541 gtcaacgatg cagccatcga tgccaatggt ttcgatttgt cgttgaatgt gtcacccgtt
     9601 tgcaccagaa atttcaaatg gattctgggt ggcaatatca cgcagttgaa caacaaggtg
     9661 acctcgctgg gtaacagaac ccatataatc aacaaattgt ccgatggagc agccatcatt
     9721 accaaagtag gagagagtcc ctatagcttc tatggttacc ggacagatgg cgattatccc
     9781 gtttattcta cggtaaaaga agcgcaatcg gcctacaaat acacggaaaa cggagtggaa
     9841 tgcagccgac ccatgcgcaa ccgcaacggg gtggaattcc aagcgggcga cattaagtac
     9901 gtcgatcaga acaacgatgg tatcatcaac gacctcgaca gagtgctgct cggctcggcc
     9961 acacccgatt tctatggcgg cttgttcaac cgctttcaat acaaaggctt tgcgatcgat
    10021 ttgaattttg tttactcgtt gggcaacgaa gcctacaacg cggtgcgtcg cattaccgag
    10081 tcttcgctgg acatgagcaa ccagagtctg gccgtattgc gccgttggac tgtggacgga
    10141 caaattaccg acatgccgcg tgccaattat tacgatttgg tgggcaacaa cgattttagc
    10201 gaccgctgga tcgaagacgc ttcttacctg aagctgcgcg acattacctt tagttatact
    10261 ttcgacaata ttttgctcgg atttatacaa ggcggcactc tttacgtcag tgggcaaaat
    10321 ctgtggtgcc tgacacctta tttgggcaac gatcccgagt tctcctattc gtacagcgcc
    10381 atgatgcagg gcgtggacta tgccaagctg gcagctccca aaaccgtaac gttcggagtc
    10441 aatcttaagt tctaagcatt aaaccatcaa gattatgaaa cagaacatga tatatcgtat
    10501 ttttgccgct tcgttgttag tgctttcgat gacggcttgt tccggttttt tcgaatccaa
    10561 aacagacgat gtgct
//
LOCUS       DAOSGL010000045        10387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5448_length_10387_cov_4.846206, whole genome shotgun sequence.
ACCESSION   DAOSGL010000045 DAOSGL010000000
VERSION     DAOSGL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5448_length_10387_cov_4.846206"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="PKY68_02540"
     CDS             complement(<1..2033)
                     /locus_tag="PKY68_02540"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23949.1"
                     /translation="MKQINKIAFILLVAIVAVACGNRTDKKFEKDLSDYSGIISAISS
                     GAIRRADPIVVVFTKDIVEKELMGKNLEQNPFVFKPEIKGKCSWKSPNTLVFKPEKPL
                     NWNTNYEASLNLEYLLKLPEKLDKKLNFSFFTAKKQLKLSYDELRLDEGGNYFLEVYI
                     NTSDRFDSHEIEQSLIVGYPHEPPSLEWEHNPETFSHTLSIRNIVRAEGNSELVLNIK
                     PIDNSYKPSINQYSFTIPGTDVFMLSSVKVKESPDQVLEIVFSDLLNQQQDLRGLVSL
                     DEQKSVKYTIDNNVLKIYPSNTLSGEFTLNISRSVENKQGKTLQKNYSQIINFGRIKP
                     AIRLLGKGVIMPQSQGLILPFSAVGLKAVDLRVIQIFPNNIVAFFQDNNIDETYKWGL
                     DRVGRTIVTKKISLEDKGAEDLTNWNAFSLDLASFIDLEPGSIYNVELGFRKTYSLYE
                     EQASEETDHYLPIDEEMLFPGQSYENTYYNYYYDWRNYENPASQAYFSPNRFVNRNVF
                     CSDLALIAKMDAKGQMSIYVSNLMTTASEAGVQLSVYDYQNQLLTRAQTDGQGYFRFT
                     PERAPFVLFAQKGNQYAYLKLDNSSAIPVANFDIDGIKAEKGIKAFFYAERDVWRPGD
                     SIYLHLILEDREQQLPEGHPINMEIYNPRGQFVKKILTLRDERCIYPFYFATS"
     gene            complement(2020..2730)
                     /locus_tag="PKY68_02545"
     CDS             complement(2020..2730)
                     /locus_tag="PKY68_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOH23950.1"
                     /translation="MYPYQIFDKTKRKLVKTVTFVCKEMFIQAMNYQLVLDKTLEEIK
                     AENKKPTLLLHVCCTPCSTYVLEYLSDYFTIALYYYNPNIFPKEEYDKRLWTLVKFVQ
                     ELKSKIRPIIEEANYEPSEFYRKIKGLENEKEGGGRCKICYALRLEEAARKAAEKSLD
                     YFTTTLSISPLKNAQLINDLGALYENKYGVHYLYADFKKKEGYKRSIELSKQYGLYRQ
                     DYCGCEFSMKNKRKNETD"
     gene            3311..5935
                     /gene="mutS"
                     /locus_tag="PKY68_02550"
     CDS             3311..5935
                     /gene="mutS"
                     /locus_tag="PKY68_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOH23951.1"
                     /translation="MSNNISNTPLMKQYFSIKAKYPDAVLLFRVGDFYETYGKDAILA
                     AEILNIALTFKTAGKTGKIEMAGFPFHALDTYLPKLVKAGKRVAICDQLEDPKKTKTL
                     VKRGVTELVTPGVSTNDAVLDHGENNFLAALHLDQKRAGVAFLDISTGEFITAEGSFE
                     YVDKLLSSFNPKEILYERSKRSLFQDTFHYNGPSFEMEDWVYTDDSAQRRLLKQFQTV
                     SLKGFGIQNMPLAIIASASVLYYMDMTQHLQLGHIQQISRIDEDNYVRLDRYTIRNLE
                     LCNTINQEGKSLLEILDKTCTPMGKRQLRRWVLFPLKDIKAIEARQEVVEFFMNNKEI
                     HQYVKERLSLVGDLERIVSKVAVGRVSPREVVHLKNALMSVADIKQYLNSQSLEITPP
                     LMSMIQAVDTCEDLCLRITTELVENPPIALNRGEVIRAGVNEELDQLRNLSTSGKEYL
                     QRLQERESQKTGISSLKVSFNNVFGYYMEVRNVHKDKVPENWIRKQTLVSAERYITPE
                     LKEYEEKILGAEERISQIESDIFNALVLDLVSFVSIIQNNAVQLSQLDCLLSFAEVSS
                     LNNYHKPQLSDSDEINIKAGRHPVIEKQLPDGEIYISNDVFMDNKSEQILIITGPNMA
                     GKSALIRQTALIVIMAQTGCFVPADEAQIGLVDKVFTRVGASDNLSLGESTFMVEMLE
                     AADILNNITPRSLVLFDELGRGTSTYDGISIAWAIVEYIHEQPKARAKTLFATHYHEL
                     NEMEKTFPRIRNYHISVKEADNKVIFLRKMRRGGSEHSFGIHVAKMAGMPPSIVNRAN
                     QILQEFETSQQGKVPAKPVSRLAETREGLQLSFFQLDDPVLSQVRDEILKVNINNLTP
                     IEALNKLNEIKKIVKGRG"
     gene            complement(5941..7044)
                     /gene="ychF"
                     /locus_tag="PKY68_02555"
     CDS             complement(5941..7044)
                     /gene="ychF"
                     /locus_tag="PKY68_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOH23952.1"
                     /translation="MALQCGIIGLPNVGKSTLFNCLSNTKAQAANFPFCTIEPNVGVI
                     TVPDERLNELAKLVNPKRVVPTTVEIVDIAGLVKGASKGEGLGNKFLGNIRQTDAIIH
                     VLRCFDDSNIAHVNGAIDPVRDKEIIDYELQIKDLETIDSRINKVQKQAQTGGDKQAK
                     VAYEVLLRYKLALEQGKSARTVQFDTKDEQKIAHDLFLLTNKPVLYLCNVDEASILKG
                     NRYVDMVREAIQEEGSELLVVAAKIESEIAEFEDAEERGLFLQELGLKESGVNRLIKA
                     AYKLLNLSVFLTAGPLEVRAWPFKTGSKAPVCAGIVHSDFEKGFIRAEVIKYEDFIRF
                     GSEQAVKEAGKMHVEGKDYVVQDGDVIYFRFNV"
     gene            complement(7084..10167)
                     /gene="polA"
                     /locus_tag="PKY68_02560"
     CDS             complement(7084..10167)
                     /gene="polA"
                     /locus_tag="PKY68_02560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOH23953.1"
                     /translation="MSTSKRLFLLDAYALIYRSYYAFIKNPRINSKGENTSAIFGFIN
                     TLEDLLKRENPSHLAVAFDPPGPTFRHTAFEAYKAQREKTPETIIWSIPYIKEILKAY
                     RIPILEVEGFEADDVIGTLSLKAEKDGFKTYMMTPDKDFGQLVSEHSFIYRPRYGSSE
                     FEVMGIEEVNKKFGLKHPCQIIDLLSLMGDTSDNIPGCPGIGIVTAQKLIAEFGDIDT
                     LLNNTDKLKGSVKEKVEKNREQILFSRFLATIKRDVPIEFVEEEFRMVPRDEKKIMNI
                     FEQLEFRTLMKRISASNSYAGSEKKPAGRPEGEKKLFTPIFVEASQPGASKKKPAGLN
                     LQTSLWDEQGNAVGPSSLPTKIKTQQNIVGNDLFSNNLSAINPDESTDSLPESILSSL
                     KNTAHHYHILDNEKDIGVFMKEFSESDFFAFSTKKQGTEAMNAQLTGIAFSKEKNKAY
                     YLPCPDNRKKCKELLNSFKPLFENKRSLKIGYDLKNEILLLRNYELLLAGPLFDILIA
                     HYLIQPELRHDLDFLSKSYLNYQCLIKEGLPELKAKKQGESQDLDAEQSLRVEQDLEV
                     EQSLSAEQNLSAKQNLSAEQDLNVKMAAEFICEQADLYLQLKSKLETEIEQEGLNNLY
                     YEIEAPLIYVLADMENSGVYIDTEALKSSSVLLTKELNGIEQEIYDLAGTTFNVNSAK
                     QVGDVLFERLRIVEKAKRTKTGQYSTSEEVLETLRSKHPIVSKILSYRGLKKLLSTYI
                     DALPLLINPKTGKIHTSFNQAVTATGRLSSSNPNLQNIPIRDDKGREIRRAFSAGPGE
                     FFLSADYSQIELRVMAHLSEDENMIEAFRLDQDIHAATAARIFKLPIEDVDQDMRRKA
                     KTANFGIIYGISTFGLAERMGVSRAEAKKLIEDYFETYPKIKAYMDHSVQIARENNWV
                     ETVFHRKRYLPDIQSRNAVVRSYAERNAINAPIQGTAADLIKIAMVKIYQAFESAQLK
                     TKMILQVHDELDFIVPEEELEQVKKIIIYEMENAASFKVPIKAEIGVGKNWLEAH"
BASE COUNT         2908 a         2174 c         2164 g         3141 t
ORIGIN      
        1 ttgcttgtcg caaaatagaa gggataaata cagcgttcat cccgcagggt cagtattttt
       61 tttacaaatt gcccccgagg attgtagatt tccatattta tgggatgccc ttcgggcaat
      121 tgttgttccc ggtcttcgag tattaaatgc aggtatatgc tatcgcccgg ccgccaaaca
      181 tcgcgctcgg catagaaaaa agccttaata cccttttccg ctttaatacc gtctatatca
      241 aaattggcca caggaatagc gctgctgttg tccagcttca agtaagcata ttgattgcct
      301 ttttgagcaa acagcacaaa gggagctctt tcgggagtaa aacggaaata accctgtccg
      361 tcggtctgag cccgggtcag gagctgattc tgataatcgt atacacttaa ttgtactccg
      421 gcttcggagg ctgtggtcat caaattactc acgtaaatcg acatctgtcc cttagcgtcc
      481 atcttggcta tcaatgccag atcggaacaa aatacgttgc ggttgacaaa acgattaggc
      541 gagaaatacg cctgactggc cggattttcg tagttacgcc aatcgtaata gtagttgtaa
      601 taagtatttt catagctctg acctggaaac aacatttcct catctatagg cagataatga
      661 tccgtttctt cggaagcctg ttcctcgtaa agcgaataag tttttctaaa gcccaattct
      721 acattataga ttgatccggg ctccaaatcg ataaaagaag ccagatccag gctgaaagca
      781 ttccagttgg tcaaatcttc ggcccctttg tcctcgagag atattttttt ggtaacaatg
      841 gttcttccca ctctgtcgag tccccattta taagtctcat cgatattgtt gtcttggaag
      901 aaagccacta tattgttggg aaaaatctgg ataacacgaa gatcaaccgc cttgagtcct
      961 actgcagaaa aaggtagaat caggccttga ctctgaggca tgatgacccc tttgcccaac
     1021 aagcgtatgg ccggctttat acgtccgaaa ttgattattt ggctgtaatt tttttgaagt
     1081 gtttttccct gtttattttc gaccgaacgg gatatattca gagtaaactc ccccgacaag
     1141 gtattggaag gatagatttt aaggacgttg ttatcgatgg tatacttaac actcttttgt
     1201 tcgtccaggg agaccaatcc tcttaaatcc tgctgctgat taagcaggtc cgaaaaaacg
     1261 atttcaagta cctgatcggg gctttctttt accttcaccg agctaagcat aaaaacatct
     1321 gttcccggta tcgtaaaaga atattgattg atcgaaggtt tgtagctatt atctattggt
     1381 ttaatattca gtactaattc gctgtttcct tccgctctta ctatgtttcg tatacttaaa
     1441 gtgtggctga atgtttcggg attatgttcc cattccaagc ttggtggttc atgcggatac
     1501 ccgacaatca gactctgctc tatctcatgg gaatcaaaac ggtctgatgt gttgatatat
     1561 acctccagaa aataattacc tccctcgtcc agtcgtaact catcgtaaga taacttaagt
     1621 tgcttttttg cagtaaaaaa actgaaattc aattttttat ccagcttttc aggtaacttc
     1681 agcaaatatt ccaaattcaa gcttgcctca taatttgtat tccaatttaa gggtttttcg
     1741 ggtttaaaaa ccaaagtatt aggtgatttc caactacact ttcctttgat ttcaggctta
     1801 aagacgaaag gattttgttc aaggtttttt cccatcaatt ccttttcgac tatgtctttg
     1861 gtaaatacca caacgatagg atctgcccgt cttatcgctc cggagctgat ggcagaaata
     1921 atgccggaat agtcactcag gtctttttca aatttcttgt ctgttctatt cccgcaagcc
     1981 acagctacaa tggcaacgag gagaatgaaa gcaattttgt taatctgttt cattttttct
     2041 tttatttttc atggaaaact cacaaccaca ataatcttgt ctgtataaac cgtattgttt
     2101 tgataattcg atagatcgtt tgtagccttc tttctttttg aaatcggcat ataaataatg
     2161 aacaccgtat ttattttcgt atagtgcccc taagtcatta ataagttgtg catttttcaa
     2221 aggactgata cttaatgttg tagtaaaata gtccagggat ttttcggccg ctttacgggc
     2281 agcttcctcc aagcgtaaag cataacatat tttgcatcga cctcctccct ctttttcgtt
     2341 ttctaaacct tttattttcc ggtaaaattc ggaaggttcg taatttgctt cttcaatgat
     2401 tggacgaatt ttggatttta attcctgaac aaattttact aatgtccaaa ggcgtttatc
     2461 gtattcttct ttaggaaata tattgggatt gtaataataa agtgcaatcg taaaataatc
     2521 gcttaaatat tccaacacat aggtactaca aggtgtgcag catacatgaa gcaaaagagt
     2581 tggtttttta ttttcagctt taatttcttc cagtgttttg tcgagaacaa gttgataatt
     2641 catggcttgt ataaacattt ctttacacac aaaagtaact gtttttacta acttccgctt
     2701 ggttttatca aaaatctgat atggatacaa tgtattttat atctttcagt tgcccatttc
     2761 gcttaaactc tcaaattcag ttatctaatc atggttttaa gaaatatgat actcgaactg
     2821 aatatcaacg cgaacttttg cagtaatttt ctgcttttga taaagtagaa aacttatttg
     2881 tcaacactaa tactattata aatttgaaat ttgaatttta aaataaaata ataaatgatt
     2941 atagtctgtt gatagtgtca ataatttaat tgaaataatc ttattaacaa tagagcgaag
     3001 ttataaagat ctaatcaaat acttattaac aatgtaaata attgaactta aatcaaataa
     3061 tagatttatc aacataataa gcccgatctt gttattttac ctgtcaaaaa cagacgttgt
     3121 tcaaaaccaa attttgtttg ttgaaaacta atttataaat aaaggattta taatttgcaa
     3181 aattgagcaa ttcgattacc cggacgattt ttgataaaac aaaatttatt tatcaaaagc
     3241 tatccataat tattgacaag atttcgacag atatcgtatt tttgcaattg catcgaaaca
     3301 accagagtcc gtgtcaaaca atatttccaa tacacccctg atgaaacagt attttagtat
     3361 caaggctaaa taccctgatg ctgttttatt gtttagagtg ggtgattttt acgaaaccta
     3421 tggaaaagat gctatactgg ccgccgaaat acttaatata gccctcacct tcaaaactgc
     3481 cggtaagaca ggaaaaattg aaatggccgg ttttccattt catgcattgg atacttattt
     3541 gcctaaattg gtaaaggcag gcaaaagagt ggctatttgt gatcaactcg aagatcctaa
     3601 aaagactaaa accttggtta aaaggggtgt tactgaatta gtaacacccg gagtttctac
     3661 caacgacgcc gtattggatc acggtgaaaa caattttctg gcggctttac atctggatca
     3721 aaaaagagca ggtgtggcct tcttggatat atcaaccggc gaatttatta cagctgaagg
     3781 cagttttgag tacgttgaca aattattgag ttctttcaat cccaaagaaa ttttgtacga
     3841 acgaagcaaa cgctctctct ttcaagatac ttttcattac aatggtccca gctttgagat
     3901 ggaagattgg gtttataccg acgattctgc acaaaggcgc ttattgaagc aatttcaaac
     3961 tgttagtctt aagggttttg gtattcaaaa catgccttta gccataatag cctcggcatc
     4021 ggtgctttat tatatggata tgacccaaca cctacaattg ggacatatac agcaaatttc
     4081 ccgtattgac gaagataatt atgtacgttt agaccggtat actatacgta atcttgaatt
     4141 atgcaatacc atcaatcagg aaggcaaaag cctgctggaa attctggaca aaacctgcac
     4201 acccatggga aaacgccagc tgagacgctg ggtattattt cctctgaaag acataaaagc
     4261 aattgaagcc agacaagagg tggtggaatt tttcatgaac aataaagaaa ttcatcaata
     4321 tgtaaaagag cgtctgagtc tggtaggcga cctggaacgt atagtgtcca aagtggctgt
     4381 gggtagagtc tcacccagag aagtcgtgca tctgaaaaac gccttgatgt cggtggccga
     4441 cattaagcag tatctcaatt ctcagtcttt ggaaatcaca ccgccattga tgtcgatgat
     4501 tcaggctgtg gacacttgtg aggacctctg tctgcgcatt acaaccgaac tggtcgaaaa
     4561 tcctcccata gccctgaacc ggggcgaggt aatacgtgcc ggagtaaatg aagaactgga
     4621 ccaattacga aatttgtcta catcgggcaa agaataccta caaagactgc aggaaagaga
     4681 aagtcaaaaa actggcataa gcagtttaaa agtcagtttt aataatgtgt tcggctacta
     4741 tatggaggta cgcaacgtgc acaaggacaa agtgcccgaa aactggatac gcaagcaaac
     4801 attagtcagt gcagaacggt atattacccc ggagcttaaa gaatacgaag aaaaaatcct
     4861 cggagccgaa gagcgcatat ctcagataga aagcgatatt ttcaatgccc tggtcctgga
     4921 tttagtcagt tttgtaagca ttattcaaaa caatgccgtc cagctttccc aactcgattg
     4981 tttgctttct tttgccgaag tttcgagcct aaacaattac cataaacctc aattaagcga
     5041 ttccgacgaa atcaatatta aagccggacg ccatcccgtc atcgaaaaac aactgcccga
     5101 tggtgaaatt tacataagta acgacgtgtt tatggacaac aaaagcgagc agattctaat
     5161 tataaccggt ccgaatatgg ccggaaaatc ggccttgata cgacagacag ccctgattgt
     5221 aattatggca cagacgggtt gttttgtccc ggcagacgaa gctcaaatag gattggtcga
     5281 caaggttttc accagggtag gggcctccga taatctttcg ctgggcgaat ctacttttat
     5341 ggtagaaatg ctcgaagccg ccgatatttt gaacaacatt accccgcgca gcctggtgct
     5401 tttcgatgaa ttaggtcggg gaaccagcac ctacgacggc atttccatag catgggctat
     5461 agtggaatac attcacgaac agcccaaagc cagggcaaaa acactctttg ctacccatta
     5521 tcacgaattg aacgagatgg aaaaaacctt tcctcgcatc cgcaattacc acatttcggt
     5581 aaaagaagca gacaataagg tgatttttct tcgtaaaatg cgtcgcggcg gcagcgaaca
     5641 cagctttggt atacatgtgg ccaagatggc cggtatgccg cccagtatag taaacagagc
     5701 caatcaaata ctgcaggaat tcgaaacaag ccaacagggg aaagtgccgg ctaagccggt
     5761 ttccagacta gccgaaaccc gggagggttt gcagttgagt ttctttcagt tggacgatcc
     5821 ggtactttcg caggtaaggg acgaaattct caaagtgaat ataaacaacc tgactcctat
     5881 tgaggcactc aataaattaa acgagataaa aaagatagtc aagggcaggg gataataagc
     5941 ctatacattg aaacggaaat atataacatc gccgtcttgc actacgtagt ctttgccttc
     6001 tacgtgcatt ttgccggcct cttttacggc ctgttccgat ccaaagcgga taaaatcctc
     6061 gtatttgatg acttctgcac gaataaaacc tttttcaaaa tcactatgga ctatacctgc
     6121 gcagacagga gctttgctgc cggtcttaaa aggccaggcc cggacttcga gaggaccggc
     6181 ggttaaaaac acactaaggt tcaacaattt ataagcagct ttaatcaggc ggttcacgcc
     6241 ggattccttt agtcccagct cttgcaaaaa taagccacgt tcttcggcgt cttcgaattc
     6301 ggcaatttcc gattcaattt tagcggccac caccagcagt tccgagcctt cttcttgaat
     6361 ggcctcgcgg accatatcca catagcgatt gcctttgagg atggatgctt cgtcaacgtt
     6421 acagaggtaa agcacaggtt tgttggtcaa caaaaacagg tcgtgggcaa ttttttgctc
     6481 gtctttggtg tcgaattgta ctgtgcgggc cgacttgccc tgttccagcg ccagcttgta
     6541 acgcagtagc acttcgtaag ctactttggc ttgcttgtcg cctccggtct gggcttgctt
     6601 ctgaactttg ttgatgcgac tgtcgatggt ttccaggtct ttgatttgca attcgtagtc
     6661 gatgatttcc ttgtccctga cgggatctat tgctccgttt acgtgagcaa tattgctgtc
     6721 gtcgaagcag cgtagcacgt gtatgatggc atcggtttgg cgaatattgc ccagaaattt
     6781 gtttcccagg ccttctcctt tgctggctcc tttgaccagt ccggcaatat cgacaatctc
     6841 gacggtagtg ggtactactc gcttgggatt gaccaatttg gccagttcgt tcaaacgttc
     6901 atcgggtact gtgataacac caacgttggg ttcaatagta caaaagggaa aattggccgc
     6961 ctgagcttta gtattggaca gacaattgaa gagagtggat ttacccacgt tgggcaaccc
     7021 tataatgccg cattgaagtg ccataaattc gaagtaaaag aggtcgcgaa agtacaaaaa
     7081 acatcaatgc gcttcaagcc aatttttacc cacgccaatt tcggccttga tgggcacttt
     7141 gaacgaagcc gcattttcca tttcgtaaat aattattttt ttaacttgtt ccagttcttc
     7201 ctcgggcacg atgaaatcga gttcgtcgtg cacttgcaga atcatttttg tctttagctg
     7261 agccgattca aaggcctgat agattttgac catggcaatt ttgatcaggt cggcggccgt
     7321 gccttgaata ggcgcattga tggcgttccg ttcggcatag gacctgacaa cggcattgcg
     7381 agattgtatg tcgggcaggt aacgtttgcg atgaaagacc gtttctaccc aattgttttc
     7441 acgggctatc tgcacagaat gatccatata ggccttgatt ttgggatagg tttcaaaata
     7501 gtcttcgatc aactttttgg cttctgcacg tgaaacaccc attctttctg ccagaccgaa
     7561 ggttgaaata ccgtatataa tcccgaaatt ggcggttttg gccttacggc gcatatcctg
     7621 gtcgacgtcc tctatgggaa gcttaaatat cctggctgcc gtggccgcgt gaatgtcctg
     7681 gtccagtctg aaagcctcta tcatattctc gtcttcgctc aggtgagcca ttacgcgcaa
     7741 ttcaatctgg gaatagtcgg ccgacaaaaa gaactctccg ggaccagccg aaaaagctct
     7801 gcgaatttct cttcccttat cgtcgcgaat ggggatgttt tgcaggttgg gattgctgga
     7861 actcagtctg ccggtggctg ttacggcctg gttaaaactg gtatggattt ttcctgtttt
     7921 ggggttgatg agcaggggta gggcatcgat gtaggtgctg agtagctttt ttaaaccccg
     7981 gtagctcaat attttactta caataggatg tttgcttctc aaggtttcca atacttcttc
     8041 ggaggtggaa tactggcctg ttttagtgcg ttttgctttc tccacaatac gcaagcgttc
     8101 gaacaacaca tccccgacct gcttggcaga gttgacatta aaagttgttc ctgccagatc
     8161 gtaaatttct tgctcaatac cgttcaattc tttggtaagc agcaccgaag agcttttgag
     8221 tgcttctgta tctatataca ctccgctgtt ttccatgtca gcgagcacgt agatgagtgg
     8281 cgcttcaatt tcgtaataca agttgtttag cccctcttgt tctatctccg tttccagctt
     8341 gcttttgagt tgcaaataca gatctgcctg ctcgcaaatg aactcggccg ccatcttcac
     8401 attcagatcc tgttccgcgc tgagattctg tttcgcgctg agattctgtt ccgcgctaag
     8461 actttgttcc acctcgaggt cctgttccac cctgaggctc tgttccgcat ccagatcttg
     8521 cgactcaccc tgttttttcg cttttagctc cggcaagccc tctttgataa ggcattggta
     8581 gttgaggtag cttttggaca aaaaatcaag atcgtgtctt aattcgggtt gaataagata
     8641 atgcgcaata aggatgtcga aaaggggccc cgccaataaa agttcataat tgcgcaagag
     8701 caatatttcg ttctttaaat cgtagccaat tttaaggctg cgcttgtttt caaaaagcgg
     8761 cttgaaagaa ttcaacaatt ccttgcattt ttttctgttg tcggggcagg gcaggtagta
     8821 ggctttgttt ttttcttttg aaaaggcaat tccggtcagc tgagcattca ttgcctctgt
     8881 tccctgtttt tttgtgctaa aagcaaaaaa atccgactcg gaaaattctt tcataaaaac
     8941 cccaatatcc ttctcattat caagaatatg gtaatggtga gctgtgtttt ttaagctgct
     9001 gagaatcgat tctggcaacg aatctgtaga ctcgtctgga tttattgccg acaaattgtt
     9061 cgaaaacaag tcgtttccca caatattttg ctgagtcttg atttttgttg gtaatgacga
     9121 aggaccaaca gcattaccct gttcgtccca aagtgaagtt tgtaaattta atccggcagg
     9181 tttttttttg cttgctcccg gttgggaagc ttccacaaaa atcggagtaa ataatttttt
     9241 ttctccctcg gggcgacctg ccggtttttt ttcagagcca gcgtaagaat tgctggcgct
     9301 gattcttttc ataagcgtcc gaaattccaa ctgctcaaaa atgttcatta tttttttttc
     9361 gtcgcgtggc accatccgaa attcttcttc gacaaattcg atagggacgt cccgtttgat
     9421 ggtagccaga aaacgggaaa aaagaatttg ttcacgattc ttttccactt tttctttaac
     9481 cgagcctttc aatttgtcgg tattattaag taaagtgtca atatctccga attcagcgat
     9541 taatttttgg gcggtcacta tgccgatacc cggacagccc ggaatgttgt cggatgtatc
     9601 gcccatcagg ctcaagaggt cgataatctg acaggggtgt ttcagaccga attttttatt
     9661 cacttcctct atgcccatca cttcaaattc cgaggagccg tagcgtggcc tgtaaataaa
     9721 ggagtgttcc gaaaccaact gtccgaaatc tttgtccggg gtcatcatat aggtcttaaa
     9781 gccatctttt tcagccttga gcgataaggt gcctataaca tcgtcggctt caaaaccctc
     9841 tacttcgagg attgggatac gataggcctt tagtatttct tttatgtagg ggatagacca
     9901 gatgatggtc tccggtgttt tttcgcgctg cgctttatag gcctcgaagg ccgtatgcct
     9961 gaaagtagga ccgggaggat cgaatgccac cgccagatgc gatgggtttt ctcttttgag
    10021 cagatcctca agggtgttaa taaaaccaaa aatcgcagag gtgttttcac cctttgaatt
    10081 gatacgagga tttttaataa atgcataata cgacctgtaa atcaatgcat aagcgtcgag
    10141 taaaaaaagt cttttcgatg ttgacatagt aaaaatgaag aaattggcct gtaaaggtat
    10201 aaaatatagc atgaaatagc ttaaaacagg aaacttgttt ttaaacagcg cttatgtgaa
    10261 tgacaggaat tatccttaaa tttgcagctt aattaattaa ctatgattga cctgattcga
    10321 cggcccatcc ataaggagtt tcttctctat caggaaacat acaagaaagc cctggaacct
    10381 gaaaacc
//
LOCUS       DAOSGL010000046        10331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5483_length_10331_cov_3.931588, whole genome shotgun sequence.
ACCESSION   DAOSGL010000046 DAOSGL010000000
VERSION     DAOSGL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5483_length_10331_cov_3.931588"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PKY68_02565"
     CDS             complement(<1..1324)
                     /locus_tag="PKY68_02565"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOH23954.1"
                     /translation="MLSLVAAVLIASLQAVYPCTNLIVTKGASADGSVMVTYSADSHQ
                     LYGELYFRPAADYPAGSMLQIYEWDSGKYLGEIPQAEHTYSVVGNMNEHQVVIGETTF
                     GGRPEFYDSTGIMDYGSLMFVALQRAKTAREAIAVIDRLMQDYGYASSGESFSIADPD
                     EVWIMEIMCKAPRYNKKGKNLNKGAVWVAKRIPDGHISGHANQARITNIDFNNPDDCL
                     FSKDLISHAREQGLFTGKDEEFSFCDVYAPADFGALRYCEARVWAYFNRFAPGMDKYL
                     NYAMGYDLNNKMPLSVEPTRKLNVKEVADMMRDHYEGTPMDMTLDIGAGGGACPYRWR
                     PMEFKVDSLTYLNERAIATQQTGWWYVAQCRSWLPRDLGGLFWFGVDDAGTSPLMPIY
                     SASLEVPECVREHNGSMLEYSPTSMFWLNNRIAQFAYLRYDAIGAEVRS"
     gene            complement(1455..2291)
                     /locus_tag="PKY68_02570"
     CDS             complement(1455..2291)
                     /locus_tag="PKY68_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOH23955.1"
                     /translation="MRIIRSKRNDTAWHLAAEEVLFQGKDAVLLLYINPPSVVIGCNQ
                     IMENESDTAYCRENNIGIYRRISGGGAVYHDEGNINYCFIQDKNLQQSSLNGAFLEPV
                     EHILQSLGLQVSRARRKDLWAEGYKISGTASHIGKGRELHHGTLLYDSNLEALHAALG
                     LIPGEKADQSPGKKQQPGSKGIVSVPSPVANIRSLYPSFAALSTQDFLAAFFEAAHCY
                     YGQSVYGQSVWDGEERGLKEGQKKRKEDEERLQGFRKEELAEIEALCRQKYRNKAWNE
                     LK"
     gene            complement(2305..3603)
                     /locus_tag="PKY68_02575"
     CDS             complement(2305..3603)
                     /locus_tag="PKY68_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOH23956.1"
                     /translation="MKQLDTKFMHPAEQINVIISRIYRSGMTTTSGGNISIKDENGDI
                     WVTPSGIDKGTLTFRDIVCVKPDGKVIGEHKPSSEYPFHKAIYDTRPEITAVIHAHPP
                     GLVAFSIARQIPNTNIIPQAKNICGEIGYAPYACPGSEQLGRNIAEVFKTLKYKAVIL
                     ENHGVVLGGTDMMDAYQRFETLEFCCRTLVNAGRLGKPRFLSDEKINEFHARIPHNIS
                     HFMDVSYPSDERFIRGLMCNIIRRSCDQGLMISTYGTVSVRWRNDDFLITSPNVPRWD
                     VQPEDIVQVRNGMAEAGKTPSRSVALHHRIYQKNPQINSIITTQPPNLMAFAVSGTKF
                     DVRTIPESWIFLQDVPSIPFGMHYEEIDQVADMLGRNRAILVDNDCVIVTGDKLLQTF
                     DYLEVAEFSAHSLVMASAIGPLQPIGEEEIEELRKAFNVK"
     gene            complement(3755..4291)
                     /locus_tag="PKY68_02580"
     CDS             complement(3755..4291)
                     /locus_tag="PKY68_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH23957.1"
                     /translation="MKKLSILEMNRLSAEDFKTAPKLPLTLVLDNVRSLHNIGSVFRT
                     SDAFLVEKVILCGISSCPPQPEIHKTALGAEFTVDWMYEEDCLQAIKALREQSYIIYA
                     AEQTTGSICLQDLRLDPGQKYALVLGNEVKGVQQEVIDLCHHCVEIPQYGTKHSLNVS
                     VASGILVWSFFEQLRKRL"
     gene            4406..5944
                     /locus_tag="PKY68_02585"
     CDS             4406..5944
                     /locus_tag="PKY68_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOH23958.1"
                     /translation="MKRVFSLLVLCLVAANTWSQDQADTNTESQDVTGSWYGRISVMG
                     HNLRLNFHISNEDGNYSATMDSPDQKAFGIPMQSATYEDQVLILKVSNITYTAKLDSA
                     GNLDGEFKQAAIKTGLYMTHQPLTDSTDQKVRRPQEPRPPFPYQSEEVRFKGGGDYQL
                     AGTIFKPRKGKNPPIAVFISGSGAQNRDQELFDHKPFLVVADYLARKGIASLRYDDRG
                     TAESGGNQYEGSSYENALDAAAAVDFLKASGYRQIGLIGHSEGGLIAPMVASIDSGIA
                     FIVSLAGPGVTGAEIIKKQSFDIAKAEGTAGDTLEVQTILAEYIFDRMAALPENEPDS
                     IAGIIEGALRQLNPEQSEIALGGMSREEYIQASVQQLGEEWMFHFLSFDPAQAWKEVS
                     CPVLALNGEKDLQVNADINLAAIQQALTEGKNPKLTTIKYPNLNHLFQNANTGSPSEY
                     ANIEETFSSKVLRDISKFIRKSTGMHQRTKLFIDGGIAALLLILLFVVLKKHRKKTTD
                     SPNV"
     gene            5954..6685
                     /locus_tag="PKY68_02590"
     CDS             5954..6685
                     /locus_tag="PKY68_02590"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HOH23959.1"
                     /translation="MIAFSPKRPVFLFYLWVVVFLLIQSPVLAEDKQSGQNKTATLTA
                     EEGHVETISAENPGRQAPGGLPGKRVAVKLNGLLLAGVVNPAVEFAVHNNISVQLEGM
                     GVFYHDHVPFLNFPLVLGLTFVEGRFYPKEVYRGFYAGPNLGWGAWKMNKSMAPGYFG
                     TYDNGSVQYGHNVMLGLSLGYQWILSNRWSIDLNLGLGWQHSTYEGYKNGVLYTDPSI
                     PESQPNRSAEWMPAYKGGLFFSYRF"
     gene            6695..8062
                     /locus_tag="PKY68_02595"
     CDS             6695..8062
                     /locus_tag="PKY68_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH23960.1"
                     /translation="MSLPKFTRAFWVANVVELLERLAYYAVFIVITLYLSNIWGFSDI
                     EAGLISGVFSASLYFLPTFVGAYADRIGFRRSIILAFALLTLGYFGLGLLPTLLESAG
                     LVSYGDTTVFSGLPESPLRWSIVPVMILIVVGGAFVKAVISGTVARETTSENRARGYA
                     IFYMMVNIGAFTGKSVVDPLRKSLGDMGLVYLNYFAAAMTLLALIAVFFLYKSSQTQG
                     EGKSLREIWRGLVKVFTNARLIVLILIISGFWMIQSQMYATMPKYVIRLIGESASPGW
                     YANVNPLVVFLLVNFITSLMAKKTALTSMTIGMAIIPLSALVMSLGHQLGNTPVLGMH
                     PVALLMVIGIAMQALAECFISPRFLEYFSLQAPKGEEGLYLGFSHLHSFISYLFAFGL
                     SGFLLDKYCPDPRLFDSAEAYAQATVNAHYIWYVFVGIGMISIVALLIYGWATKKNDR
                     RKQTV"
     gene            complement(8120..9373)
                     /locus_tag="PKY68_02600"
     CDS             complement(8120..9373)
                     /locus_tag="PKY68_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOH23961.1"
                     /translation="MKTNVVKVKLWGTNVGYLSWDSREGVAVFEYDPSFLKLGWDIAP
                     LTMPVNASRSRKQMPWLGNKDTLYRGLPPMIADSLPDKWGNSLFNSWLRDNKISTKNI
                     TPIDHLSFIGSRAMGALEYEPAQKLGDDSPFSVDVQRLYHFAKQVLNERESTVLTEEN
                     SILWQDLVKVSSSPGGKRPKAIVALNDDTGEVISGQGTIPEGFLHYILKYDDSSIFPY
                     AKLEYIYYKMAVDTGIAMMPSDLRTYEGATHFLTRRFDRIGNDKIHTQTLAAMSPLST
                     SYEDIFATIRRLNLPYEDSRQQYLRMVFNVLARNVDDHSKNVSFCMNRDGVWRLSPAY
                     DVTFSVDLAAPAYVNKQSLTINGKNENITREDLEAVARNNDILEYNTLIDGVKASLST
                     FEEYADRLNISKSLVNRIVNDFVRV"
     gene            complement(9370..9666)
                     /locus_tag="PKY68_02605"
     CDS             complement(9370..9666)
                     /locus_tag="PKY68_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH23962.1"
                     /translation="MYSDLYEKSGSEIIRELGKRYAGYRKRMGFTQKEIASKSGLSLF
                     TISSFENGSLTGITIASFIKLLRAIDRLEEIEKLLPELPESPRVLFKKQQKNKV"
     gene            complement(9822..>10331)
                     /locus_tag="PKY68_02610"
     CDS             complement(9822..>10331)
                     /locus_tag="PKY68_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH23963.1"
                     /translation="EKETGTIEQINVTPVSRLMFILSKLLPYWIIGIFVLTMAMIVAH
                     LVYGLIPVGSVGLIYFGAVLFILIISGFSLTVANFSDTMQQTMFVMFFFIMIFMLMSG
                     LLTPIDSMPAWAQKITFILPPRYFVDILRSVYLKGTSLVEQWYNFAALGVFTVIFNIL
                     AAVTYRKQA"
BASE COUNT         2535 a         2599 c         2438 g         2759 t
ORIGIN      
        1 tgcttcgtac ctcagctcca atcgcgtcgt agcgtaaata ggcaaactga gctatccggt
       61 tgttgagcca gaacatcgaa gtaggtgaat attcgagcat cgatccgttg tgctctcgta
      121 cgcattcggg cacttcgagc gaagcgctgt agataggcat caaaggagag gtgccggcat
      181 cgtccacccc aaaccagaac aagccgccca ggtcgcgggg caaccaactg cggcactggg
      241 ccacgtacca ccaaccggtt tgttgggtgg ctatggctct ttcgttcagg taagtcagac
      301 tgtctacttt gaactccata ggcctccaac gataggggca ggcgcctccg ccggctccga
      361 tgtcgagggt catgtccatg ggagtgcctt cgtaatgatc gcgcatcatg tcggccactt
      421 ctttcacatt cagttttcgc gtgggctcta ccgacaaagg cattttattg ttgagatcat
      481 agcccatggc gtaattcagg tatttgtcca tgccgggggc aaaacggttg aaataggccc
      541 aaacccgggc ttcgcaataa cgcaaagccc caaaatcggc cggcgcatat acgtcgcaaa
      601 agctgaattc ttcgtccttg cccgtaaaga ggccttgttc acgggcatga ctgattaggt
      661 ctttgctgaa caggcaatca tcgggattat taaaatcgat gttggtaatt cttgcctgat
      721 tggcgtgtcc gcttatatgt ccgtcgggaa tgcgcttggc tacccaaacg gctcctttat
      781 tcagattttt ccctttcttg ttgtaacgag gagccttgca cataatttcc ataatccaga
      841 cctcgtcggg gtcggcaatg gaaaaagatt ctcccgaaga agcgtagccg taatcctgca
      901 tcaatcggtc gatgacggca atggcttcgc gggcggtttt ggctctttgc aatgccacaa
      961 acatcaaaga accgtagtcc ataatgcccg tggaatcgta aaattccggc cggcctccga
     1021 aagtggtttc gcctatcacc acctgatgct cgttcatatt accgacgacc gaataggtat
     1081 gctctgcctg ggggatttca cccaggtact taccgctatc ccattcgtaa atctgaagca
     1141 tggagcctgc cggataatcg gctgcgggcc gaaaatacaa ttccccgtac aactggtggg
     1201 aatcggccga gtaagtgacc attaccgaac cgtcggcaga agcccctttg gtcacaatca
     1261 aattcgtgca gggataaacc gcttgcaggc tcgcaatcag gacagccgca acaaggctga
     1321 gcatactttt cttcatactc ccgggatttc tatttattct tttatccatc ttatatctct
     1381 tttgtttgtc gcttactatt ttactttttt ccttgtgttg ttttctcttc cctttacttc
     1441 tctgcttatc agggttattt caactcattc caggctttgt tccggtattt ttgcctgcag
     1501 agggcctcta tttcagccaa ttcttctttt ctaaaacctt gaagcctctc ctcatcttct
     1561 tttcgtttct tttgtccctc ttttaagcct cgctcttccc catcccaaac agactgtccg
     1621 taaacagact gtccatagta gcagtgggcc gcctcgaaaa aagcagctaa aaaatcctgg
     1681 gtggacagag ccgcaaacga agggtataaa cttcggatat tggctaccgg tgaaggcacg
     1741 gaaactattc ctttgcttcc cggctgttgc tttttccccg gcgattgatc tgctttttct
     1801 ccgggaatca ggcctaaagc tgcgtgcaag gcttccagat tgctgtcgta caatagagtg
     1861 ccgtgatgca attccctgcc tttgccgatg tgcgaagcgg ttcccgaaat tttgtatcct
     1921 tccgcccaga ggtctttgcg acgggctctg ctcacttgca gacccagcga ttgcagaata
     1981 tgttccaccg gctccagaaa agctccgttc agggaggatt gttgcagatt tttgtcctga
     2041 ataaagcagt agttgatgtt gccttcgtcg tgatagaccg cacctccgcc ggaaatgcgg
     2101 cgataaatac ctatgttgtt ttcccggcaa taggccgtat ccgactcgtt ttccataatc
     2161 tggttacaac ctattactac cgaagggggg ttgatataca gcaacaaaac cgcatccttg
     2221 ccttggaaaa gtacttcttc ggcagccaaa tgccaggctg tgtcgttgcg cttgctgcgt
     2281 ataattctca taggttcagc cgccttattt tacgttgaaa gccttgcgca attcttcgat
     2341 ctcctcttcg ccgatgggtt gtaaaggacc tatggctgaa gccattacca gtgaatgggc
     2401 actaaattcg gctacttcga ggtagtcgaa agtctgcagt aatttatcgc cggtcacaat
     2461 gacgcaatcg ttatcgacca ggatagcccg gttgcggccc aacatatcgg ctacctgatc
     2521 gatttcctcg tagtgcatcc caaaaggaat gctgggcaca tcctgcaaaa agatccagct
     2581 ttcgggaatg gtccggacat cgaatttggt acccgaaacg gcaaaagcca tcaggttggg
     2641 cggctgagtg gttatgatgg aattgatctg cggatttttt tgataaatgc gatgatgcaa
     2701 agccaccgac cggctgggag tcttgccggc ttcggccata ccgtttctga cctgcacaat
     2761 gtcttcgggc tgcacatccc aacgcggcac gttgggcgaa gtgatcaaaa agtcgtcgtt
     2821 gcgccaacgt accgacacag taccgtaagt ggaaatcatc agaccctgat cgcaggagcg
     2881 gcggataatg ttgcacatca aaccgcgaat gaacctttcg tcggagggat aggacacatc
     2941 cataaaatgg ctgatgttgt ggggaatcct agcgtgaaat tcgttgatct tttcgtcgct
     3001 caaaaacctg ggtttaccca gcctgccggc gttgaccaag gtacggcaac aaaactcgag
     3061 ggtttcgaat cgctgatagg cgtccatcat gtcggtaccc cccaacacca ctccgtgatt
     3121 ctcgagtata acggccttgt attttaaggt tttgaacact tcggcgatgt tacggcccaa
     3181 ctgctcgctg cccggacaag cataaggagc gtagcctatt tctccgcaaa tgtttttggc
     3241 ctgggggatg atattggtgt tgggtatctg ccgggcaatg ctaaaagcca ccaatccggg
     3301 gggatgagca tgaatcactg cagtaatttc gggacgggta tcgtagatgg ccttgtgaaa
     3361 aggatattcc gacgagggtt tgtgttcgcc gataacttta ccgtccggtt taacgcagac
     3421 tatatccctg aaagtcaggg ttcctttgtc tatacccgaa ggggtgaccc aaatatcgcc
     3481 gttttcgtcc ttgatagaaa tattgcctcc ggaggtggtg gtcatgccac tgcgataaat
     3541 gcggctgata atcacattga tctgttcggc cggatgcatg aatttggtat ctaattgctt
     3601 catattggaa ttatctgatg gttctgttat tgaaatccgc tactgttaat gagtctgtta
     3661 ctgttactgt tactgttact gttatttatt gttattgtta ttgttattga gtctgtttct
     3721 gttaccgaat ctgccggcat tattacatct gctattacag acgttttctc aattgttcaa
     3781 aaaagctcca gaccagtatg cccgacgcca ccgacacatt gagcgaatgt ttggttccgt
     3841 attgaggtat ttccacgcaa tgatggcaga gatcgatcac ttcctgctgt acgcctttca
     3901 cctcgttgcc cagcaccagc gcatactttt gtccgggatc tagccggaga tcctgcaaac
     3961 aaatactgcc cgtggtctgt tcggctgcat aaataatata actctgttcg cgcaaagctt
     4021 taatcgcctg caagcaatcc tcttcgtaca tccagtctac tgtaaattca gctcccagag
     4081 ctgttttgtg aatctcgggc tggggcggac aggagctaat accacagagg atgacttttt
     4141 ccaccagaaa agcatccgag gtacgaaaca ccgaaccgat attgtgcaag ctgcgcacat
     4201 tatccaggac caatgtcagg ggaagcttgg gcgccgtttt aaaatcttcc gccgataggc
     4261 ggttcatttc gagtatactg agttttttca ctgtctaaca tgtgataccg ggcaaaagta
     4321 cgattttttt aaataccggt ctatatatta agcctaagag cgttttactt cgggatttgt
     4381 tatatttgca aaaaatatca aactcatgaa acgcgtattt tctttgttgg tgctgtgcct
     4441 ggtggcagca aacacatgga gtcaggatca ggccgataca aacacagaga gtcaggatgt
     4501 taccggaagt tggtatggcc gcatttccgt tatgggacac aacctgcgcc ttaacttcca
     4561 tatcagtaac gaagacggga actattcggc caccatggac agtcccgacc agaaagcctt
     4621 tgggattccc atgcaatcgg ccacctacga agaccaggta ctcatcctga aagtctccaa
     4681 tataacctac accgccaaac tggattctgc gggtaatctc gacggcgagt ttaagcaagc
     4741 cgctatcaag accgggctgt atatgacgca tcagccattg accgacagca cggatcaaaa
     4801 agtacgacgt cctcaggagc cccgacctcc tttcccctac caatccgaag aggtccggtt
     4861 caaagggggt ggagactatc aactggccgg caccattttc aagccccgta aaggcaaaaa
     4921 tcctcccatt gcggtgttta tttccggcag cggagcccaa aaccgcgatc aggaactgtt
     4981 cgaccacaag ccctttctgg tcgttgccga ctacctggcc cgcaagggta ttgcctcttt
     5041 gcgttacgac gaccggggaa cggcggaatc gggcggtaat caatacgaag gcagcagcta
     5101 cgagaatgcc ctcgacgccg cagccgctgt tgatttcctc aaagcatcgg gctaccgaca
     5161 aatcggattg atcggacaca gcgaaggagg actgattgct cccatggttg cctccatcga
     5221 ctccggtata gcctttatcg tttccctggc cggcccgggt gtgacgggtg cggaaatcat
     5281 caaaaaacaa agcttcgata tcgctaaagc cgaaggaaca gccggggata ctctcgaggt
     5341 tcaaacaata ctcgccgaat acatcttcga tcgtatggcc gccttgccgg aaaacgagcc
     5401 ggacagcatc gccggtatca tcgaaggggc tttgcggcaa ctcaatcccg agcagagcga
     5461 aatagccctg ggcggtatga gtagggaaga atacatccag gccagtgtac aacaactggg
     5521 cgaggaatgg atgttccatt ttctcagctt tgatcccgca caagcctgga aagaagtgag
     5581 ttgtccggta cttgccctga atggagaaaa agatcttcag gtgaatgccg acatcaacct
     5641 ggcagccata cagcaggcat tgacggaagg aaaaaatccc aaactcacca ccattaaata
     5701 ccccaacctc aaccacctgt ttcaaaacgc caacaccgga tctccttccg aatacgccaa
     5761 tatagaggaa actttctctt ccaaagtgct tagagacatc agcaaattca tccgcaagtc
     5821 caccggtatg caccagcgaa caaagctttt cattgacggc ggaattgctg ccctcttgct
     5881 gattttgctc tttgttgtgc ttaaaaaaca tcgaaagaaa acaaccgact ctcccaacgt
     5941 ttaagctgct tgtatgattg cattctctcc caaacgcccg gtatttttat tctacctctg
     6001 ggtcgtcgtc tttcttttaa ttcaaagccc cgttctggcc gaagacaagc aaagcggaca
     6061 aaacaaaacg gccactttaa ccgcagaaga gggacatgtc gaaaccatct ctgctgaaaa
     6121 cccgggccgg caagcccccg gcggacttcc cggcaaacgc gtagccgtca aactcaacgg
     6181 tctgctgctg gccggtgtgg taaatccggc cgtggaattt gccgtacaca acaacatcag
     6241 cgtgcaattg gagggcatgg gggtgtttta ccacgatcac gtgccttttc tgaactttcc
     6301 actggttttg ggccttactt tcgtcgaagg acgtttttat cccaaagagg tttaccgagg
     6361 tttctacgca gggcccaacc tgggctgggg tgcctggaaa atgaacaaaa gcatggctcc
     6421 gggttatttc ggtacctacg acaatggctc ggttcaatac ggtcataacg tgatgctggg
     6481 gctgagcctg ggctatcaat ggatcttgtc caatcgctgg tcgatcgatc tgaacctggg
     6541 gctggggtgg caacattcca cctacgaagg ctataaaaac ggagtattgt ataccgaccc
     6601 cagcattccc gaaagccagc ccaaccgcag cgctgagtgg atgccggctt acaagggagg
     6661 actctttttt tcttatcgtt tttaataccg ttttatgtct ctaccaaaat ttaccagagc
     6721 cttttgggtt gccaatgttg ttgaactgct cgagcgtctg gcctactacg ccgttttcat
     6781 tgttataacc ctctacctgt cgaacatctg gggcttctcc gatatagaag ccggcctgat
     6841 ttcgggcgtt ttttcggcca gtttgtattt tctgcccaca tttgtaggtg cttacgccga
     6901 tcgcataggt tttcgccgat ccattatcct ggcttttgcc ctgctcaccc tgggttactt
     6961 tggcctgggc ctcttgccca ccctgctcga aagcgccggg ctggtaagtt acggcgacac
     7021 caccgttttc agcggcctac cggaatctcc cctgcgttgg tctattgttc cggtcatgat
     7081 tttgatcgtc gttggcggag cctttgtcaa agcggtgatt tcgggcacgg ttgcccgcga
     7141 aaccacttcc gaaaaccggg cacggggtta cgccatcttc tacatgatgg tcaacatcgg
     7201 agcttttacc ggaaaatcgg tggtcgatcc actgcgcaaa agcttaggcg atatgggcct
     7261 ggtttacctg aactattttg ccgcagccat gactctgttg gcgctgattg ccgttttctt
     7321 tctgtacaaa tcttcccaga ctcagggcga aggcaaatca ctgcgtgaaa tctggcgggg
     7381 attggttaaa gtctttacca atgcccggct gatcgtgttg attctcatta tcagcggatt
     7441 ctggatgata caaagccaaa tgtacgccac catgcccaag tacgtgatcc gcctgatcgg
     7501 cgaaagcgct tcacccggct ggtacgccaa cgtaaacccc ctggtagtgt ttttgctggt
     7561 caattttatc acttcgctga tggccaaaaa aacggccctc acttccatga ccataggcat
     7621 ggccatcatt ccgctctcgg cgctggttat gtcgctggga caccagcttg gaaatactcc
     7681 cgttttggga atgcacccgg ttgccctgtt gatggtgatc ggtatcgcca tgcaagccct
     7741 ggccgaatgc tttatttcgc cccgttttct ggagtatttt tccttgcagg ctcccaaagg
     7801 cgaagaaggc ctttacctgg gattcagcca tttacattcc ttcatttcct atttgttcgc
     7861 cttcgggctc tcgggttttc tgctcgacaa atactgcccc gacccccggc tcttcgattc
     7921 ggccgaagcc tacgcccaag ccacggtcaa tgcccattac atctggtatg tttttgtggg
     7981 tatcggcatg atttcgatcg ttgccctgct tatttacggc tgggccacca aaaagaacga
     8041 tcgcaggaaa cagacggttt aagaagaggt tttagacgct gattggggtt gttgattcag
     8101 aatttaatga gaataaaact cacaccctta caaaatcatt cacaatccga ttaacaaggg
     8161 atttacttat attcagtcga tcagcatatt cttcgaatgt tgaaagggaa gctttcacgc
     8221 cgtcaatcaa cgtgttgtat tcgaggatat cgttgtttct ggcgactgct tccaagtctt
     8281 cacgggtgat attttcgttt ttgccgttaa tcgtcaggga ttgcttgttg acgtaggcgg
     8341 gagctgctaa atctacgcta aaagtaacgt cgtatgcagg cgataagcgc caaactccat
     8401 cgcgattcat gcaaaacgac acatttttgg agtgatcgtc cacattgcgg gccaacacat
     8461 taaacaccat tcgcaaatat tgctgacggc tgtcttcata gggcaaattg agtcgccgga
     8521 tggttgcgaa aatatcttcg tagcttgtac ttagcggaga catcgcggcc agggtttggg
     8581 tatgtatttt atcgttaccg atacggtcga aacgacgggt aaggaaatgt gtcgctccct
     8641 cgtaggttct caaatcggac ggcatcatcg caattcccgt atcaacagcc attttgtaat
     8701 aaatatattc cagcttggcg tagggaaaaa tcgaactgtc gtcgtactta agaatgtaat
     8761 ggagaaatcc ttcgggaata gttccctgtc cggagataac ttcgcctgta tcgtcgttca
     8821 atgccacaat cgctttgggg cgctttccac ccggcgagga gcttaccttt accaagtctt
     8881 gccacaaaat ggaattttcc tcggttaata cggtggattc tctttcgttt aaaacctgct
     8941 tcgcaaaatg gtagagtctt tgaacatcaa ccgaaaaggg cgaatcatct cccagttttt
     9001 gtgcgggttc atactctaat gcccccattg cccggctgcc gataaacgaa agatggtcaa
     9061 tgggtgttat gttttttgtt gaaattttat tatcgcgcaa ccacgaatta aaaagggaat
     9121 taccccattt gtcgggcaat gaatcggcaa tcatcggagg aagccccctg tacagtgtgt
     9181 ctttgttgcc tagccaaggc atttgcttac gactgcggga cgcgttaacc ggcattgtga
     9241 gaggggctat atcccatccc agcttgagaa aagaaggatc gtattcaaaa acagccaccc
     9301 cttccctgct atcccaggaa agatacccga cattcgttcc ccaaagcttt actttaacga
     9361 catttgtttt cataccttat ttttttgctg ttttttgaac aatactcttg gactctcggg
     9421 taattcgggc aagagctttt cgatttcttc caaacgatct atcgctcgaa gtaatttaat
     9481 aaacgatgct atggttattc ccgttaaaga gccgttttca aatgaactga tggtaaacag
     9541 gcttaaaccg gattttgaag caatctcctt ctgcgtaaac cccatgcgct tgcggtaacc
     9601 ggcatatcgt ttccccaatt cccgtatgat ctcgcttcct gatttttcgt acaaatcgct
     9661 atacatttcc agttatattt aatcacaaaa tagatatatc tatattattt cgcaaatata
     9721 tgactattta ttgatatatc tattccattt ggggagaaaa gcttgatttg tgaaagagct
     9781 ttacaagtaa gttatgcgaa attaagcccg gctatgcaag tttaggcctg cttcctataa
     9841 gtaacagcag cgagaatatt gaagataacc gtaaaaacac cgagagctgc gaagttgtac
     9901 cattgttcga caaggcttgt tcccttcaga tagactgaac gtaatatatc gacaaaataa
     9961 cgcgggggca gaataaaggt gattttctgc gcccaggcgg gcatggaatc aatgggcgta
    10021 agcagtccgc tcatcagcat aaagatcata ataaagaaga acataacgaa cattgtctgt
    10081 tgcatcgtat cggagaaatt ggccaccgtg aggctgaatc cggaaataat cagaataaac
    10141 aataccgcac caaagtaaat aagcccgaca gaaccgacgg gtataagccc atagacgaga
    10201 tgcgcaacaa tcattgccat cgtaagcacg aatatgccta taatccaata aggtaacagc
    10261 tttgacaaaa tgaacataag gcgactcacg ggtgtaacat tgatctgttc aatcgtgccg
    10321 gtttcctttt c
//
LOCUS       DAOSGL010000047        10226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5569_length_10226_cov_3.871104, whole genome shotgun sequence.
ACCESSION   DAOSGL010000047 DAOSGL010000000
VERSION     DAOSGL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5569_length_10226_cov_3.871104"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PKY68_02615"
     CDS             <1..241
                     /locus_tag="PKY68_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOH23964.1"
                     /translation="MVIMPILGESFLQLISGDLIAATGSSLPLSSLLIGVVTAFVSGC
                     IACKWMLNLVKKGKLIWFAIYCAVVGLLALILPLV"
     gene            244..969
                     /gene="truB"
                     /locus_tag="PKY68_02620"
     CDS             244..969
                     /gene="truB"
                     /locus_tag="PKY68_02620"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOH23965.1"
                     /translation="MNFQEGEILYFNKPLYWTSFKLVNEVRYVLCKALKIKKLKVGHA
                     GTLDPLATGVMIICTGKATKRIEEFQHLSKEYIATLQLGATTPSFDLETEIDERFAYA
                     HIDEAQVQETLKQFIGEIRQVPPMFSACKVQGKRAYEMARKGEHIELESKLLVIDELE
                     LLEHNLNKRAETQEEPFIKIRVVCSKGTYIRALARDIGRALGSGAHLTALVRSRIGEI
                     GLDDCRTIEEFKKELEEQLIQNI"
     gene            966..2015
                     /gene="queA"
                     /locus_tag="PKY68_02625"
     CDS             966..2015
                     /gene="queA"
                     /locus_tag="PKY68_02625"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOH23966.1"
                     /translation="MKLSKFKFNLPESLIAQYPAPHRDESRLMVLDRESGKIEHKIFK
                     EILEYFDEEDVFIFNDAKVFPARLYGNKEKTGAQIEVFLLRELNPELRLWDVLVDPAR
                     KIRIGNKLYFGEDESMVAEVIDNTTSRGRTLRFLFDGDHEVFKQTLYSLGETPLPKYI
                     QRPVEPDDAERFQAIFAKNEGAVVAPAANLHFSRELMKRMEIKGVNFAFITLYVGLGG
                     FREIDVEDLTKHKMDSERIMVEEEAANIVNEAKDKGKKVCAVGTSVMRTIESSVGAAG
                     HLKPYDGWTNRFIFPPYEFSVANCMLANFHMPYSTLLMMASAFGGYELIKGAYELAVK
                     EKYRFGCYGDALLIL"
     gene            2023..>2456
                     /gene="folK"
                     /locus_tag="PKY68_02630"
     CDS             2023..>2456
                     /gene="folK"
                     /locus_tag="PKY68_02630"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOH23967.1"
                     /translation="MAKLYLGLGSNLGDRAHYLDKAIAEIQLRIGPVIKRSEYYPSEP
                     WGYKSSNTFLNACICVETTLHWRKCLELTQEIELTLGRTISKRVSDNTKLAGQTEDKG
                     QTEDKEQTEGKEQTEVKGQAEDKDKDKDKDKDKDKRAAYQDR"
     assembly_gap    2457..2556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2557..2792
                     /gene="folK"
                     /locus_tag="PKY68_02635"
     CDS             <2557..2792
                     /gene="folK"
                     /locus_tag="PKY68_02635"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOH23968.1"
                     /translation="DKDKDKDKDKRAAYQDREIDIDLLLYDRLVLNEPDLVLPHPLMH
                     KRKFVLLPLSQIAPELQHPVLERSVQELLSALD"
     gene            3048..5003
                     /locus_tag="PKY68_02640"
     CDS             3048..5003
                     /locus_tag="PKY68_02640"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOH23969.1"
                     /translation="MKKPLFLVLSMFTGLIALNAKEIQLQSPDGNLQIVVTIDKEISW
                     SAQLKGKPVLEKAVVAMELSDRVLGLEARLAKQSVKEVKQEIQAAVPYKDAIIEDHCN
                     ELSLQFRERFSLTFRAYNDGVAYRFSETSRKPYEVLNEKMDLFFPDGAACYFSEEHTT
                     YSHNESWYPYIQLSDIAAGRFCSMPLLIDLPGQAKVLVTETALHRYPGMFLQSNGRNG
                     FVSKFPPYVLETRNPARGADRNEIISKEADYIAKVATGRDFPWRVFIISGKDADLVES
                     NLTYQLAEPCVLKDTEWIKPGKVAWDWYNANNIFGVDFKSGLNTATYKYYIDFASDNK
                     IEYVILDEGWTKSTTEILENNPNIDVKELIEYGRQKSVGIILWVLWKPLNANMKQILE
                     TYSGWGAKGVKVDFMQRNDQYMVESYEQIAAECARLNLLVDYHGSFKPSGLSRKYPNV
                     LSQEGVKGNENNKWCADVTPEHNLILPFTRMVAGPMDYTPGAMLNAHPVNHNVSYDRP
                     MGLGTRCHELAKYIVYESPLQMMCESPSIYKKEQESVDFITRIPVVWDETRVLEAKVS
                     DYIVVARRKGKAWYVGAMTDASPRELEISLSFLDEGDYKLTLMKDGINADRYAQDYKL
                     EKRSINKTEVLKIKMVSNGGWAAILEP"
     gene            complement(5021..6784)
                     /locus_tag="PKY68_02645"
     CDS             complement(5021..6784)
                     /locus_tag="PKY68_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOH23970.1"
                     /translation="MKPTPFFSGVFKLPALLLLFAVITPPLYAQKAVKASGTPLFTDF
                     EYRGNDKIYQNHPLKPDEFYNPILQGCYPDPSITRKGDDYYLVCSSFAMFPGVPIFHS
                     RDLVNWTQIGHVLDRKSQLIIHNCGISAGIYAPQIIYNPYNDYFYMITTQISGGIGNM
                     VVKTKDPHQGWSEVIKLDFNGIDPSLFFDDDGKAYVVHNDGPDRGKELYNGHRVIKIW
                     EYDTEHDKIIPGSDKIIVDGGVDISKKPIWIEAPHIYKKNNKYYLMCAEGGTGGWHSE
                     VIFVSDSPKGPYIPAPGNPILTQRHFARDRANKVDWAGHADLVLGPDNKTYYGVFLAV
                     RPNEKNRVNTGRETFILPVDWSGEFPVFVNGLLPMEPKLKMPQGVTENNTGKENFLPN
                     GNFSFSDNFSAGKPDYRWISLRGPREEFIETSKQGLKIKPYPVNIKEVKPTSTLFLRQ
                     MHNSFSFTVGMNYRPSSEKELAGLVALQSESFNYVFGLTKKGNDYYLLLERNERKARS
                     RETNSTILASNMIELKAPIRLRIRAEGDNYEFSYSLDGADFVNLAENVSGDILSTDVA
                     GGFTGCLLGLYATTANDAVPE"
     gene            complement(6839..7996)
                     /locus_tag="PKY68_02650"
     CDS             complement(6839..7996)
                     /locus_tag="PKY68_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOH23971.1"
                     /translation="MKKTSFTLVFLLISYFSFAQFAGMMRGQQAEALPEGFKPSSTNT
                     FLALYPAVNAQTRQALFKVVAPTANNVQVDLCNKKYDMTKDDRGVWTCMSDPQVVGFH
                     YYAILIDGVAVMDRNTKAFFGSNWMSSGIEIPEGPEGDYYRFDKNIPHGQVRSIYYWS
                     EINGMERHVNVYVPAEYEQKTDKSYPVLYLVHGWGEDENGWSNQGRMANIMDGLIASG
                     KAVPMIVVMPSGDIQTNPDVRRASGDISQIFVQDLLPFIDRNFRTKTDKQNRAMAGLS
                     RGGFQTCQTVSTNLDKFGWMGTFSGFFIRGDNSLETAFNGLFKDPSEFKRQIKLLYIS
                     TGTEENTPKAQVEALKAKGIDNIVYSESQGTAHEWLSWRRALNEFAQLIFK"
     gene            complement(8050..9420)
                     /locus_tag="PKY68_02655"
     CDS             complement(8050..9420)
                     /locus_tag="PKY68_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH23972.1"
                     /translation="MISNSKLLFVCLSLLASISGGLFIPLAAQNPIIRDQFTADPSAR
                     VFDGKIYVFPSHDIRAPEGKNLRKDWFCMEDYHVFSSENLIDWTDHGVILSQYDPPWV
                     DSASYSMWAPDCIERNGKYYFYFPAHTKADENGRRSLGIGVAVADSPEGPYLPQTEPI
                     KGARGIDPNVFIDKDGQAYLYWSQQHIYVAKLKENMLELDSEPYIIPNLPEKGLKEGS
                     WLFEREGIYYLTFPHVENKTERLEYAVGKHPMGPFTMTGVIMDESPTACWTNHHSILE
                     YWGQWYLFYHHNDYSPDFDKNRSIRIDSLFFNEDGTIQKVQPSLRGVGLTPANTEIQL
                     DRYSALSPDGAEIAYIDKNRPFEGWKTILNKPGAWVQYNAIDFGNKKKKRVQIRALAP
                     QGGILELRLNHPGGPLLAGLTLPAGSDWENYHFKIKNTGRGIDNLIVLLRSESELEID
                     WLQFQP"
     gene            complement(9433..>10226)
                     /locus_tag="PKY68_02660"
     CDS             complement(9433..>10226)
                     /locus_tag="PKY68_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOH23973.1"
                     /translation="DERWFAYLFRDYGSVGRIPYLVPIQWQDGWPVLGIEGKVPDELD
                     LPAGKGLKPGIVNDDEFNRKAGEPELPLVWQWNHNPDNNLWSLSERPGYLRLKTGRID
                     KSFLLARNTLTQRSFGPQSSGSTLLDVSGMKDGDVAGLVALQRKFGQVGVKMEGSNKY
                     IFMTSNKTDTPTEIERLPLKKKKVYLRIDCDYREMTDLARFYYSLNGKTWKEIGRPLK
                     MEYTLVEHFMGYRFGLFNYATKQSGGWADFDYFHIDDQIIENNEQ"
BASE COUNT         2637 a         2286 c         2583 g         2620 t
ORIGIN      
        1 tatggtgatt atgcccatat tgggagaatc gtttttgcag ttgatatcgg gcgatctaat
       61 tgccgccaca ggcagtagtt tgcctttgag ctctcttttg ataggcgtag tgaccgcctt
      121 tgtttcgggt tgcattgcct gcaaatggat gctcaatctg gttaagaaag gcaaactcat
      181 ctggtttgcg atatactgcg ccgttgtggg tttattggcc cttattttac cattggtata
      241 attatgaatt ttcaggaggg agaaattctg tattttaata agccgttgta ctggacatcg
      301 tttaaattag taaacgaagt acgctacgtc ttatgtaaag ccttgaaaat taaaaagctt
      361 aaagtgggcc acgccggaac cctcgatccc ttggccaccg gcgtaatgat tatttgtacc
      421 ggtaaagcca ccaaacgtat agaagaattt caacacctga gcaaggaata tattgccact
      481 ttgcaactgg gagccacaac accatcgttc gacttggaaa cagagattga tgagcgtttt
      541 gcgtatgctc atatcgacga agcgcaggtg caggaaacct tgaagcaatt cataggagaa
      601 atccgtcagg tgccacctat gttttcggcc tgtaaggttc agggaaaaag agcttacgag
      661 atggcgcgca aaggggaaca tattgaattg gagtccaagc tgttggtcat cgacgaattg
      721 gaattgttgg agcataatct gaataaaagg gcagaaacgc aggaagagcc ttttattaag
      781 attagggtag tatgcagcaa aggtacttac atcagggctt tagccagaga tattggccgg
      841 gctttgggta gtggggccca tctgactgcc ctggtacgtt cgaggatcgg agagattggt
      901 ctcgacgact gccgtacgat cgaagagttt aaaaaagaac tggaagaaca attaatacaa
      961 aacatatgaa actgtcaaaa ttcaaattta acctgccgga gagtctgatc gctcaatatc
     1021 cggctcccca cagggacgag tctcgcctga tggtattgga tcgcgaaagc ggtaaaattg
     1081 aacacaagat ttttaaagaa atcctggaat attttgatga agaagatgtg ttcattttca
     1141 acgatgccaa ggtgttccct gcccgtttat acgggaataa ggaaaaaacg ggggcacaga
     1201 tcgaggtgtt tttattaagg gagttgaatc ccgaattacg tctttgggac gtgctggtgg
     1261 atccggcccg caaaattcgc atcggcaaca agctatattt cggcgaagac gaatccatgg
     1321 tggccgaagt tatagacaat actacatcca gaggacgtac cttgcgtttt ttgttcgacg
     1381 gcgatcacga ggtttttaaa cagacacttt actctctggg cgaaacgcca ctgcctaaat
     1441 acatacaacg tccggtagaa cccgatgatg ccgaacgttt tcaagccatt tttgccaaaa
     1501 acgagggtgc ggtagttgct ccggcagcca atttgcattt cagtcgcgag ctgatgaagc
     1561 ggatggaaat caaaggagtg aattttgcat ttatcacgct ttacgtcgga ctgggaggct
     1621 tcagagaaat cgatgtcgaa gacctgacca agcataaaat ggactcggaa cgaataatgg
     1681 tcgaagaaga agccgccaac attgtgaacg aagccaaaga caagggcaaa aaggtttgtg
     1741 ccgtcgggac ttccgttatg cggaccatcg agagctccgt cggagctgcc ggccacctta
     1801 aaccctacga cggctggacc aatcgcttta tttttcctcc ctacgaattc agtgtggcca
     1861 attgtatgct ggccaatttt catatgcctt attcaaccct gctgatgatg gcttccgctt
     1921 ttggcggtta tgaactcatc aagggagcct acgagttggc tgtcaaggaa aaataccgtt
     1981 ttggatgcta cggagatgcc ttgctgattc tttaaaacgg acatggccaa gctctattta
     2041 ggcctgggat ctaaccttgg cgatcgtgcg cattatctag acaaggctat tgccgaaatt
     2101 caattgcgca tcgggccggt aattaagcgt tcggagtatt acccgagtga gccctggggt
     2161 tacaaatctt ccaatacttt tttgaatgcc tgtatttgtg tggaaaccac ccttcattgg
     2221 cgaaaatgcc tggaattgac acaggagata gagttaacgc tgggcagaac gataagcaag
     2281 agagtaagcg acaacacaaa actcgcaggg caaaccgaag acaaagggca aaccgaagac
     2341 aaagagcaga ccgaaggcaa agagcagacc gaagttaagg gacaagccga agacaaagac
     2401 aaagacaaag acaaagacaa agacaaagac aaaagagccg cctatcagga ccgggannnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaga caaagacaaa gacaaagaca
     2581 aagacaaaag agccgcctat caggaccggg aaatagacat cgacctcttg ctgtacgatc
     2641 ggctcgtttt gaatgagccc gatcttgtgt tgcctcatcc tctcatgcat aaacgtaagt
     2701 ttgttttact accgctcagc caaatagctc cggaattaca acatcccgtt ctggaaagaa
     2761 gtgtacaaga attattgtcg gctctcgatt agcgctttct cgtttatcaa agaccttatt
     2821 ttttgaaaag ggataaagag gttttacttg attccgggat aagcctccat gcggggatgg
     2881 cgtcaatgga tgttttatat ggcagagagc ttcaaagagg gtcaaatcct cactattaat
     2941 agcaaggtat ccataaaaaa ggactaaaat gacgtagaaa atcattaaat tgcgcagcag
     3001 aataagcgta aaaccgtgaa aaaagtcact taataaaaca aatcgatatg aaaaaacctc
     3061 tttttttagt tttaagcatg tttaccgggc ttattgccct caatgccaaa gaaattcaac
     3121 tacaatcacc cgacgggaac ttgcagattg tggtaactat tgataaagaa atttcctggt
     3181 cggcgcaact taagggcaag cccgtgctgg aaaaggccgt cgttgccatg gaactttcag
     3241 accgggtttt gggtctggag gccaggctgg caaaacagtc cgttaaagaa gttaagcagg
     3301 aaattcaagc agccgttccg tataaggatg cgattataga agatcattgc aatgaactga
     3361 gcttacagtt cagggaaaga ttcagtctga ccttcagggc ctacaacgat ggtgtggcct
     3421 accgtttttc ggaaacttcg cgtaaaccct acgaggtgct gaacgagaaa atggatttgt
     3481 tctttcccga cggagcagcc tgttattttt cggaagaaca cacaacctat tcgcacaacg
     3541 aaagctggta tccctatatt cagctgagcg atatcgctgc cggacgtttt tgcagcatgc
     3601 ccttgctgat cgatttgccg ggacaggcta aagtactcgt gacagaaact gcgctgcatc
     3661 gttatcccgg tatgttcctc caaagcaacg gccggaatgg tttcgtaagc aaatttccgc
     3721 cttatgtgct ggaaacccgg aatccggctc gcggagcaga ccgaaacgaa atcatcagta
     3781 aagaggcgga ttatattgct aaagtagcca ccggcagaga ttttccctgg cgggtgttta
     3841 tcatcagcgg caaggacgcc gatctggtgg aaagcaacct tacttaccaa ttggccgaac
     3901 cttgtgtact caaagatacg gaatggatca agccgggcaa agtggcctgg gattggtaca
     3961 atgccaacaa tatttttgga gtggatttta aatcgggcct caataccgcc acttacaagt
     4021 actacatcga ttttgcctcg gacaataaga tcgaatacgt gattctcgac gaaggctgga
     4081 caaagtcgac taccgaaatt ctggagaaca accctaacat cgacgtaaaa gaattgatcg
     4141 aatacggcag gcaaaagagt gtcggaatta tcctctgggt attgtggaag cccctcaatg
     4201 ccaatatgaa acaaattctg gagacctaca gcggctgggg agccaagggt gtcaaggtgg
     4261 attttatgca gcgcaacgac cagtatatgg tggagtcgta cgagcaaatt gccgccgaat
     4321 gtgcccgttt gaacttattg gttgattacc acggatcatt caaaccttcg ggtctcagtc
     4381 gcaaataccc caacgtgctc agccaggagg gagtcaaggg caacgaaaac aacaaatggt
     4441 gcgccgacgt tacgcctgaa cacaatctta ttttgccttt tacccgcatg gtggccgggc
     4501 ctatggatta tacccccgga gcgatgctca acgcccaccc cgttaatcac aacgtaagct
     4561 acgaccgccc catgggtctg ggcacccgtt gccatgagct ggccaagtac attgtatacg
     4621 agtcgcctct gcaaatgatg tgcgaatcgc cctctattta caagaaggag caggaaagcg
     4681 ttgattttat cacccgcatc cccgttgttt gggacgaaac ccgtgtcctt gaggctaaag
     4741 tatccgatta cattgtggtg gcacgtcgca aaggcaaggc ctggtatgtg ggagccatga
     4801 cggatgccag cccccgggaa ctggaaattt cgctttcttt cctcgatgaa ggcgattaca
     4861 agctgaccct gatgaaagac ggaatcaacg ccgaccgcta cgcccaggat tacaaactcg
     4921 agaagcgcag cattaataag accgaagttc ttaaaatcaa gatggtttcg aacggcggct
     4981 gggcggctat actcgaacct tgagccggtg atccccaacc ctattccggc acggcatcgt
     5041 tggccgtggt ggcatagagg ccgagcaaac aaccggtaaa acctcccgcc acatcggtcg
     5101 agaggatatc tccggaaacg ttttcggcca ggttcacaaa atcggcacca tcaagtgagt
     5161 agctaaattc gtaattatca ccctcagccc ttatccttag acggataggg gctttgagtt
     5221 caatcatgtt gcttgccaga atggtcgagt ttgtttcgcg gcttctcgct ttgcgctcgt
     5281 tgcgttccag caagagataa tagtcgtttc cttttttggt gagaccaaag acgtagttga
     5341 agctttcgct ttgcagggct accaggccgg ccagctcttt ttcggacgaa ggccggtagt
     5401 tcatgccgac cgtaaaggag aaactgttgt gcatttgccg gagaaagagg gtggaggtcg
     5461 gtttgacttc tttgatatta acgggataag gtttgatttt aagcccttgt tttgaagtct
     5521 ctataaattc ttcgcggggt cctctcaggc ttatccagcg ataatccggc ttgccggcgc
     5581 tgaagttgtc gctaaagctg aaattgccgt tggggaggaa gttttccttg ccggtgttgt
     5641 tttcggtaac accctgaggc atctttagtt taggttccat cggaagcagc ccgtttacaa
     5701 agacggggaa ttcacccgac cagtcgacgg gaaggatgaa ggtttcgcgc cccgtgttca
     5761 cgcggttctt ttcgttggga cggacagcca aaaagacccc gtaataggtt ttattgtcgg
     5821 gcccgagcac caaatcggca tgacctgccc aatctacttt gttagcccta tcccttgcaa
     5881 aatggcgctg agtcaggatg ggattgccgg gcgccggaat ataagggcct ttggggctgt
     5941 cactcacaaa aattacttcg ctatgccagc ctcctgttcc gccttcggca cacattaggt
     6001 agtacttgtt gttttttttg taaatatgag gggcttcaat ccaaatgggc tttttggata
     6061 tatccacgcc tccgtccacg atgattttgt cgctgccggg aatgattttg tcgtgctcgg
     6121 tatcgtattc ccagatttta attaccctgt gtccgttgta gagttcttta ccacggtcgg
     6181 ggccgtcgtt gtggaccaca taagctttgc cgtcgtcgtc gaaaaacaga gaggggtcga
     6241 tgccgttgaa atccagttta atcacttcgc tccatccttg gtggggatcc ttggtcttga
     6301 cgaccatatt gccaatgccc ccggaaattt gggtggtgat catataaaaa taatcgttgt
     6361 aaggattgta gattatctga ggggcataga tgccggcgct gataccgcaa ttgtggatta
     6421 tcagttgcga tttcctgtcg agcacatggc ctatttgcgt ccagttgacc aggtcgcggg
     6481 aatggaaaat gggtactccg ggaaacatgg caaaagagga gcaaaccaaa taataatcgt
     6541 ctcctttgcg agtgatgctc gggtcggggt aacaaccctg aagaatggga ttgtaaaact
     6601 cgtccggctt cagaggatgg ttctggtaaa tcttgtcgtt gccccggtac tcaaaatcgg
     6661 taaacagggg agtcccggaa gctttgactg ctttttgagc atagagcggg ggagtgatta
     6721 cagcgaataa taagagcagg gccggtagtt taaaaacacc ggagaagaat ggagtaggtt
     6781 tcatacgttg aaataatggt tgttgttttt ttattaacaa gatttgaacc gggtcggatt
     6841 acttaaagat caattgggca aattcattca acgctctgcg ccagctgagc cattcgtgag
     6901 ccgttccctg agattccgaa taaacaatgt tatcgatgcc cttggctttg agggcttcaa
     6961 cctgagcttt gggtgtgttt tcttcggtgc ctgtgctgat gtagagcagc ttgatttgcc
     7021 gtttgaattc cgaaggatct ttaaacaaac cgttgaaagc cgtctcgagg ctgttgtcgc
     7081 cccttataaa aaatccgctg aaggttccca tccagccaaa tttatcgagg ttggtggaga
     7141 ccgtctggca ggtttgaaag ccacctctcg aaaggccggc catggcgcgg ttttgtttgt
     7201 ctgttttggt tctgaagttc cggtcgataa aaggcagtaa atcctgaaca aatatctgac
     7261 tgatgtctcc actggcccgg cgaacgtcgg gattggtttg aatgtcaccg ctgggcataa
     7321 ccacgatcat gggcaccgcc tttcccgagg caataagtcc gtccataatg ttggccatgc
     7381 ggccttggtt ggaccagccg ttttcgtctt cgccccaacc atgtaccaga tagagtacgg
     7441 gatacgactt atcggttttt tgttcgtatt cggccggaac ataaacgtta acgtggcgtt
     7501 ccattccgtt gatctcggac cagtaataga tggaacgaac ctgaccgtgg ggaatgtttt
     7561 tgtcgaaacg gtagtagtcg ccctctggcc cttcggggat ttcgatgccc gacgacatcc
     7621 agttgctacc gaaaaaggct ttggtgttgc gatccatcac cgctacgccg tcgatgagaa
     7681 tagcgtaata gtgaaaaccg accacctgag gatcggacat acaagtccat acccctctgt
     7741 cgtctttggt catgtcgtat tttttattgc acaaatccac ctgaacattg ttggcggtgg
     7801 gagccaccac tttaaagagc gcctggcggg tttgagcatt gacggccggg tacaaagcca
     7861 ggaaggtatt ggtcgaagaa ggtttgaagc cttcgggcag ggcttctgcc tgttgcccgc
     7921 gcatcatgcc ggcaaactgg gcaaagctga aatagcttat cagcagaaag actagagtaa
     7981 aagaagtttt tttcatgatt taaactatta aagttggtgt tcaattaggt agatagtcaa
     8041 atttatggtt tagggttgaa attggagcca gtcaatttcc agctcagatt ccgaacgaag
     8101 caaaacgatc aggttgtcta tacctcgccc ggtattttta attttaaagt ggtagttttc
     8161 ccagtcagag ccggccggga gtgtgagacc tgccaaaaga ggcccgccgg ggtggttgag
     8221 ccttagctcg aggatgccgc cttgtggtgc cagagctctg atttgtactc ttttcttctt
     8281 tttattgcca aaatcgatgg cgttgtactg cacccaggct cccggcttgt ttaatatcgt
     8341 tttccagcct tcgaaaggcc tgtttttgtc gatataagcg atttcggcac catcgggact
     8401 gagggcagaa taccggtcaa gctgaatttc ggtattggct ggggttaatc ctactccacg
     8461 cagactgggc tgtacttttt gaatagtccc gtcctcattg aaaaaaagac tgtcgatacg
     8521 gatggagcgg tttttatcaa aatccggcga ataatcgttg tggtgataaa acaaatacca
     8581 ctggccccag tattccaaaa tggaatgatg gttggtccag caagccgtgg gcgattcgtc
     8641 cataataacc cccgtcatgg taaagggacc cataggatgc ttaccaacag cgtattcgag
     8701 gcgttccgtc ttgttttcaa catgggggaa agtcaggtag taaattccct ctctttcaaa
     8761 taaccaagac ccttctttaa gtcctttttc cggcaaattt gggataatat aaggttccga
     8821 atccagttca agcatattct ccttcaattt ggccacataa atatgttgtt gtgaccaata
     8881 taggtaggct tggccgtctt tgtcgatgaa aacgttcgga tctattccgc gtgctccttt
     8941 gatgggttcg gtctggggca ggtaagggcc ttcggggctg tcggctacag ccactccgat
     9001 gccaagactt ctgcggccgt tttcatctgc tttggtgtgg gccggaaaat aaaagtagta
     9061 tttcccgttt cgctctatgc aatcgggggc ccacatgctg tacgaagcag aatcgaccca
     9121 gggcggatcg tactggctga ggatcacacc atggtcggtc caatcgatga gattttcgga
     9181 cgaaaaaacg tggtaatctt ccatacaaaa ccagtctttg cgcaggttct tgccttccgg
     9241 tgctctgatg tcgtgcgaag ggaaaacata gatctttccg tcgaaaaccc gggccgaagg
     9301 gtcggccgta aactgatccc ggataatcgg gttttgcgcc gcaagcggga taaacagtcc
     9361 gcccgaaatg gaagccaaaa ggctcaggca aacaaaaagc aatttggagt ttgaaatcat
     9421 tgtttatgct ctttattgtt cattgttttc aataatttga tcatcgatgt ggaaataatc
     9481 gaagtcggcc cagccaccgc tttgtttggt ggcgtaatta aagagtccga agcgataacc
     9541 cataaaatgc tctaccaggg tgtattccat tttgaggggc cgaccgattt ctttccaggt
     9601 cttgccgttc aggctgtaat agaagcgagc cagatcggtc atttccctgt aatcgcagtc
     9661 gattctgagg tagacctttt tctttttcag cggcagcctt tcgatttcgg tgggtgtgtc
     9721 tgttttgttg ctggtcataa aaatatattt gttgctgcct tccattttga ctcccacttg
     9781 tccgaatttg cgctgcaggg caaccaatcc ggctacgtcg ccgtctttca ttcctgaaac
     9841 atcgagcagg gtgctgcccg aactttgggg accaaaactg cgctgagtca gcgtgtttcg
     9901 tgccagtaaa aaactcttgt ctatgcgtcc cgttttcaag cgtaaatagc cggggcgttc
     9961 acttagcgac cagagattgt tgtcgggatt gtggttccat tgccagacaa gagggagctc
    10021 aggttcaccg gctttacgat tgaattcatc gtcgttgaca atgcccggtt tcagaccttt
    10081 accggccggc agatccagtt cgtcgggcac tttgccctcg attccaagca ccggccagcc
    10141 gtcttgccat tgtatcggaa ccagataagg gatccggcct accgaaccgt agtcgcggaa
    10201 aagataggca aaccagcgct cgtcgg
//
LOCUS       DAOSGL010000048        10135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5647_length_10135_cov_3.968254, whole genome shotgun sequence.
ACCESSION   DAOSGL010000048 DAOSGL010000000
VERSION     DAOSGL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5647_length_10135_cov_3.968254"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKY68_02665"
     CDS             complement(<1..296)
                     /locus_tag="PKY68_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23974.1"
                     /translation="MVKHGFILILVCFFPLCIQAHSLLEGRVCDQQGNGLFSVLVCLK
                     QQKNSCCYSDTKGYFSLDIPDTATDDSLFLFFVGYKPLSIPLKELDLSSFAELRL"
     gene            complement(272..1438)
                     /locus_tag="PKY68_02670"
     CDS             complement(272..1438)
                     /locus_tag="PKY68_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23975.1"
                     /translation="METATWQWNEDFLILHNNNSELILKNDSNGALVSLSEAEYIFLH
                     YYSIDNDYEKVCSLMQDYHIEKDLFDALTNKARQLGLLIRPGSTRKTPKKSSGLKITA
                     YFLFVRFLGIIGKLCGLNLIAECKGNLRFYKLCSLDLHNTVLHKMALSERLQKILLPL
                     YILTMVLLISLLWLLPGAEIRFSGFGMTHIPIFGFFLLLVVSIFACLFIHETGHYLCY
                     MRYGGKSSKMGLGLMFLVFPVMYTHIDQVQLWHDKRRKIKLSLAGVLMDVMIMLLLLN
                     LLSHYHKPDLFALLIACLFYYYAVQLLSNLNPFFPGTDGYYVFEDLIGGERLFGNSYE
                     CFKGFVKHPFAEKISLKRCGYLLFFLIACINISLYWFMIAALMTFPLWSNMVLS"
     gene            complement(1446..2261)
                     /locus_tag="PKY68_02675"
     CDS             complement(1446..2261)
                     /locus_tag="PKY68_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HOH23976.1"
                     /translation="MDIANLFYDQGLSQYALDFMQSSSMTPFNQLENAQRIQSIMNIP
                     YYSRQIIAHTLERGTTERIKLNALFNEESIALRNIQIKRKSTRSFHEHINLEELANLL
                     LNSYFITERFEKHSHHLSRRSIPSGGALYPVDLYYINLNTTGLQRGVFVYNPHEEALE
                     LLQPAGDKKNFFRQLRKCFPEAVLGSWNLDMISGIVVFGGVLNRSSCKYGDRGLRFVL
                     MDVGALCQNLHLSAAAGGVSCCAIGGYIDQNLNDFLGFQQPDECALLSMFIGK"
     gene            complement(2267..2983)
                     /locus_tag="PKY68_02680"
     CDS             complement(2267..2983)
                     /locus_tag="PKY68_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23977.1"
                     /translation="MKQLFLLLLLAAVLPLQAQNLQTEIDSAFIHYFKTGRMEEIDRI
                     INALHLENKSSNNRYLNYWTAYGRYKKSLCAQSNPAEEFQKTARIELSEALKCLNKIK
                     NKESEDYALLAILKNYSVAFVASVKIPFVSGEAKKYAQKAIDMDPQNLRAYLALGIRD
                     YYTPAVYGGKTQFEELFLKAISLRDKYGNNPNDPSWGKNDAYYYLLDHYCRKDMEKAR
                     HLFSEAIELYPDDPRILKFK"
     gene            complement(2994..4277)
                     /locus_tag="PKY68_02685"
     CDS             complement(2994..4277)
                     /locus_tag="PKY68_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HOH23978.1"
                     /translation="MKTICHTAAGLLSEAMIRIPNRRFVPDGLVQSVVSGKNLDWMNC
                     RRREIQGSSIAFDEEQSYRGALGEFLERYACATYDSNDFKAASYSELSKSEPALAPEF
                     FRYYSDEQYERLRELNVYPLGENDLIEWTVCNDFITGKSYWMPAFSIYMPYFSKVNSP
                     HNYMVGTTSTGTAAGKTSRDALISGFLECAERHAFALFWYHQDALPYRSYTTEVILRH
                     YHKNKTICRLFQNSAVQIKSFDLAAFSPVECMVVFLYFRYKNKIYQSLGCAARFNKTQ
                     ALIKACQEAYQGVEYAISLNEKKLLPEEPDLSRIDDFDKHFHFYNQYPQFRKEAPILR
                     EAARFDSGDEKIYRSDPDNKCMDFTVEELKKVGLKHLIYKDISPIDAEELQYKIMRVV
                     TPGWSLLTGNHNWPFLGYCLNGNQTLFTAYPHPFP"
     gene            complement(4280..5134)
                     /locus_tag="PKY68_02690"
     CDS             complement(4280..5134)
                     /locus_tag="PKY68_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23979.1"
                     /translation="MKIKIQNMDIWECRDAIYCKRSDGNLIKIQSKANHKIIEVLKHL
                     RSVASLEDFQSEFVSGAEFSEIVKFLLSNNLIYKECEQKKQLKKIGFYGNKDVFKHLG
                     SDLKTEQFELSFNPVYENNSVQGLHLVLTISPVFDHYSLLQDLSFRSYKSMIPVLYAE
                     FSPSTLTLGPLVVPAMNTPSLGCYMKRKAVNLSSLALYSEFILSEEKQRFHNVDIRSY
                     AHYLAGMQLIATELERFWSFKGKLSTHLMGKSYTIDFLQYKTEETQILKDPASSLFKR
                     PAFSPFNA"
     gene            complement(5231..5440)
                     /locus_tag="PKY68_02695"
     CDS             complement(5231..5440)
                     /locus_tag="PKY68_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23980.1"
                     /translation="MKTLEMTMKVVEDRSAPLMLTERNLPGMCCCTCCCSCCFGTGEG
                     NGGSSSVTSPADLGKTLSTNKNIQV"
     gene            complement(5672..6340)
                     /locus_tag="PKY68_02700"
     CDS             complement(5672..6340)
                     /locus_tag="PKY68_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOH23981.1"
                     /translation="MEQLLDKIAYCVEYGKINLASPYPPDMRGQEGADEYTKRALEQG
                     VSPSDVLTKGLIVGMGKIGVKFRENKVFVPQVLMSARAMSTAMNHLKPYFNSGAVKRK
                     GKFIIGTVKGDLHDIGKNLVSMMVEGSGFEVIDLGVDVPAEKFIEAIQAHPGAYVGMS
                     ALLTTTMASMEEINKAIKQASPQTTTFVGGAPVNDEFRKQIGADYYSPDPQGLTEILT
                     KLTA"
     gene            complement(6402..7481)
                     /locus_tag="PKY68_02705"
     CDS             complement(6402..7481)
                     /locus_tag="PKY68_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23982.1"
                     /translation="MTYIKNPAFSNEPLPVEIVFHPSWWHKHCGIDFDEDFFYHPLKR
                     VESERRMEKELYERFGQYGLGSDHDKDLPQIGAVHNAAGYLLSEMLGCEIRYFADSAP
                     QVIPAQKADFHVDVDAAFAGPAFKRLEKLMSELKSRYGYLKGDVNWGGILNLAMDLRG
                     EEILMDFLLQPDEVKLYFKKIAELIERFFCFIQQETGSNSISVNRNVINITPAVYLHS
                     ECSHTMISEEQYEEFLLPFDVEWSKKYRPYGVHYCGPDPHRHAAQMAKIPHLDFLDAG
                     WGGDMVCLRKHLPSTFINIRLDPVSINTMSCETLEAHIRRLVNDCANPWLTGICCINM
                     DDKVTDDKIHTIFSTAQQLRREYRL"
     gene            complement(7566..>10135)
                     /locus_tag="PKY68_02710"
     CDS             complement(7566..>10135)
                     /locus_tag="PKY68_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH23983.1"
                     /translation="INKRRAVKLHQGAFYRFYADVSDRVFFGDKENLLEVTVSKESEN
                     SSVNLAERRADYWNFGGIFRPVFIQALPAQSIDRTAIDAKADGSFYAEVFLSTALEGD
                     YSVETQLLDREGKNIGKALKAELRPGSDKVIVKGQFTDIKTWTAETPHLYDVQFSLLK
                     GKEAVHIVKEQRIGFRTIEVKESDGLYINGQKVMIKGVNRHSFRPETGRTLSREDNYN
                     DVRLMREMNLNAVRLSHYPPDPAFLDACDELGLYVMNELGGWHGKYDTEVGKQLVKEM
                     VTRDLNHPSIIWWSNGNEGGWNTELDGEFHPWDPQKRPVLHPQGNFGGFETMHYRSYG
                     ESQEYMRKPEIFMPTEILHGLYDGGHGAGLWDYWVMMRRHPRCAGAFLWVFADEGLMR
                     TDQNGRIDNVGNYAADGIVGPHHEKEGSFFTVKEVWCPVQIKALGRTVTCTERTEALR
                     QDQNSFIADPEAFVGKFHVENRYDFISLEDCRIKWSLANFAKPGDKSGGHNIIGQGEQ
                     AGPKLAPHQSGEFQLKLPANWKEADVLYVSVLNPRGEEIYTWGWYWKSERDYTRAALA
                     GQANTSPAKLSQSREGNTLTIESGTTRLQFDTETGYLIQISKGNNNLSFSGGPRFIAA
                     RRSDRSMDVFYNHDDKAAKSKDRIYADISGNSRLIAFEVEEQEDKILFKTQYFGNLNS
                     VCWTITDREEITVDYEYQYEGVVELAGICFDYPEDNMQGMRLLGKGPYRSWQNRMHGT
                     LFDVWDKEYNDPIPGESFIYPEFKGYYNDWKWVDFQTAQAQLWLGNGTPGSFLGVYTP
                     RDGRDALLYTIPQSGIAVLDVIPGVRNKVNSTDLVGPSSQAKWLSGPQKGSLQIQIR"
BASE COUNT         2576 a         2310 c         2390 g         2859 t
ORIGIN      
        1 agtctgagct ctgcaaaaga ggaaagatca agttctttca gcgggatgct caaaggttta
       61 tatccaacaa aaaacaggaa caggctgtcg tctgtggccg tatcgggtat atccagactg
      121 aaatatcctt tggtgtcgga gtaacaacaa ctatttttct gttgttttaa acaaacaaga
      181 actgaaaaaa ggccgttccc ttgttgatcg catacccggc cctcgagcaa agaatgggcc
      241 tgaatacaca aagggaaaaa gcatacaagg atcaggataa aaccatgttt gaccataagg
      301 ggaatgtcat taaagccgca atcataaacc agtataggct gatattgatg caggcgatca
      361 aaaaaaacag taaataccca cagcgtttca gagatatttt ttccgcgaaa ggatgtttta
      421 caaagccctt gaagcactcg taagaattac cgaacaagcg ttccccgccg atcaggtctt
      481 caaacacata atagccgtcg gtgccgggga aaaacggatt cagattgctc aacaattgaa
      541 ccgcataata ataaaacaag caggctatca gcaaggcgaa cagatcgggt ttgtggtagt
      601 gggacaggag gttgagcaac aatagcatga tcatgacatc catcagaact ccggccagac
      661 tgagtttgat ctttcgccgt ttgtcgtgcc acaactgcac ttgatcaatg tgggtgtaca
      721 tgaccggaaa taccagaaac atcagtccca ggcccatctt gctgcttttc cctccgtaac
      781 gcatataaca aagataatgt ccggtttcgt ggataaacag acaggcaaaa atactcacta
      841 ccaaaagcag gaaaaatcca aaaataggta tgtgagtcat tccgaaaccg gaaaatcgta
      901 tttctgcccc cggcagtaac cacagtaagc tgatcaacaa aaccatcgtc aagatataga
      961 gaggcagcaa aatcttttgc aaccgttcgg aaagggccat tttatgcaag acggtgttgt
     1021 ggaggtccag agagcataat ttgtaaaaac gcagattacc tttacactct gcaatgagat
     1081 ttagcccaca gagttttcct atgattccta agaagcggac gaagaggaaa taagctgtta
     1141 tttttaaacc tgaacttttc ttcggggttt ttctggtgct gcccggcctg atgagcagac
     1201 ccaattggcg agctttgttg gtgagcgcgt cgaataggtc tttttctata tggtaatctt
     1261 gcattaaact acagaccttc tcataatcgt tgtcaatgct gtaataatgt aaaaaaatat
     1321 attctgcttc ggacaggctt accaaagcac cgttgctgtc gtttttcagg attaattccg
     1381 agttgttgtt atggagaatc aaaaagtctt cgttccattg ccaggtcgct gtttccatgg
     1441 ttttgtcatt tacctataaa catactgagc aaagcacatt cgtcgggttg ttgaaaaccc
     1501 aggaaatcgt tcagattttg atcgatgtaa ccacctatag cgcagcagga tactccgccg
     1561 gctgctgccg acaaatgcaa attctgacaa agggccccca cgtccatcaa tacgaaacgc
     1621 aaaccccggt caccgtattt gcaggaagat ctgttcaaca cccctccgaa aacaacaata
     1681 ccggaaatca tgtccagatt ccaggaaccc agcactgctt cgggaaaaca ttttctcaat
     1741 tggcgaaaga aatttttttt atccccggcc ggctgcaata attccaaggc ttcttcgtgg
     1801 ggattgtaaa caaaaacgcc tctttgtaag cctgtggtat tcagattgat ataatacaaa
     1861 tccaccggat aaagagcgcc acccgaggga atgcttctgc gcgacaaatg gtggctgtgt
     1921 ttctcgaagc gttcggtaat aaagtaggag ttgagcaaca agttggccaa ttcttccaga
     1981 ttaatgtgtt catgaaaaga acgggtgctt ttgcgcttaa tctgaatatt tcgcagagca
     2041 atgctttctt cgttaaataa agcattcagc ttgatccttt cggtagttcc cctttcgagc
     2101 gtatgggcaa taatttgccg gctgtaatag ggaatgttca ttatactttg aatccgttgg
     2161 gcattttcca gctgattaaa aggagtcatg gaggaacttt gcataaaatc cagtgcgtat
     2221 tggctcagtc cttggtcgta aaaaagattg gcaatatcca taactattac ttgaatttca
     2281 agattctggg atcgtcgggg taaagctcta tggcctcgga aaacaagtgc ctggcctttt
     2341 ccatgtcttt gcggcaataa tgatccaaca aataataata agcgtcgttt ttgccccaag
     2401 agggatcgtt gggattgttg ccgtatttgt cgcggagtga aattgccttc agaaagagct
     2461 cctcgaattg ggttttccct ccgtaaacgg ccggcgtata ataatctctg atgcccaaag
     2521 ccaggtaggc cctaaggttt tgaggatcca tatcgatggc tttttgggca tactttttgg
     2581 cttctccgct cacaaatgga atttttacac ttgctacaaa agccacgctg taatttttga
     2641 ggatggccaa taaagcgtag tcctccgatt ctttgttttt gattttattc agacacttca
     2701 gggcttcgga caattcaata cgggccgttt tctgaaattc ttcggccgga ttggattggg
     2761 cgcacagcga ctttttgtac cggccatagg ccgtccagta attcaggtag cgattgtttg
     2821 aacttttgtt ctccagatgg agagcattga taattcggtc gatttcttcc atcctgccgg
     2881 ttttaaaata atgaataaat gcggagtcga tttccgtttg caggttttgt gcttgcagag
     2941 gtaatactgc agctaacaaa agcagtaaaa agagttgttt catacaaata agtttaaggg
     3001 aaggggtgag gataagctgt aaataaggtc tgattaccgt taaggcaata tcctaaaaag
     3061 ggccagttgt gattaccggt caacagagac catcccgggg ttactacccg cataattttg
     3121 tattgcagtt cttctgcatc tattgggctg atgtctttgt atataaggtg tttaagtccg
     3181 acctttttga gttcttcaac ggtaaaatcc atgcatttat tatcgggatc ggaacgatag
     3241 attttttcat cgcccgaatc aaaacgtgcc gcctctctga gtatgggcgc ttcttttctg
     3301 aattgggggt attgattgta aaaatggaaa tgcttgtcga aatcgtcgat ccggctaaga
     3361 tccggttctt ccggtaataa tttcttttca ttcaagctga tggcgtattc aacgccctga
     3421 taagcttcct ggcaggcttt gatcagagcc tgagttttgt tgaaacgcgc tgcacagccc
     3481 aaggattggt aaatcttatt cttgtagcgg aagtaaagaa agaccaccat acattctaca
     3541 ggagaaaaag ccgccaggtc aaaactttta atttgaacag ccgaattttg aaagagccgg
     3601 caaatcgttt tgtttttatg gtaatgcctc aaaataacct ctgttgtgta agagcggtaa
     3661 ggtaaggcat cctggtgata ccaaaacagg gcaaaggcat gtctttcggc acattcaaga
     3721 aaaccgctta tcaacgcgtc ccggcttgtt ttgccggctg cggttccggt ggaggtggtt
     3781 cctaccatat aattgtgagg gctgtttacc ttgctaaagt agggcatgta tatgctaaaa
     3841 gctggcatcc agtacgactt tcccgtgata aaatcgttgc agacagtcca ttcaataaga
     3901 tcgttttcgc ccaaagggta cacattcaat tcccggagcc tttcgtattg ctcgtcggaa
     3961 taatagcgaa agaattccgg agccagggcc ggttcgcttt tggaaagctc ggaatagctg
     4021 gctgctttga aatcattaga atcgtaagtg gcacaggcat aacgttccaa aaattctccc
     4081 aaggccccgc gataactttg ttcttcgtca aaagctattg agctgccctg gatctcccgg
     4141 cggcggcagt tcatccagtc caggttttta cccgatacta cgctctgcac cagaccgtcc
     4201 ggtacgaaac gacgattggg tattcttatc atggcttcgc tcagcaagcc cgctgcggta
     4261 tgacatatcg ttttcattgt caagcattaa aggggctgaa agccggtctt ttgaacaagg
     4321 acgaagccgg atctttaaga atttgtgttt cttcggtttt atattgcaga aaatcaatag
     4381 tgtaagattt tcccatcaag tgagttgata atttaccttt gaaactccag aaacgctcca
     4441 gttcggttgc gatcagttgc atgcctgcaa ggtaatgagc ataggagcgt atatcgacgt
     4501 tatgaaaacg ttgtttttct tcggacagga taaattccga ataaagagcc aagctggaca
     4561 agttgacggc cttgcgtttc atgtaacaac cgagtgaagg cgtattcata gccgggacga
     4621 ccagtggtcc taaagtaaga gtgctgggcg agaattcggc gtacagaaca gggatcatac
     4681 ttttgtaact tcggaacgaa agatcctgca agagcgaata gtgatcaaaa acaggactta
     4741 tggtcaaaac aagatgcagc ccttgcacgc tattattctc gtatacagga ttaaaactga
     4801 gttcgaactg ctccgttttg aggtcggatc ctaagtgctt gaatacatct ttgtttccgt
     4861 agaatccgat tttcttcagt tgcttttttt gttcacactc tttgtatatc agattgttgc
     4921 ttagtagaaa ttttacgatt tctgaaaact ctgctccact gacaaattcc gattggaaat
     4981 cttccagcga ggcgacactg cgaagatgtt tcaaaacttc tattatttta tggttggcct
     5041 tactttggat ttttatcaga tttccgtcgg agcgtttgca gtaaatggca tctctgcatt
     5101 cccagatgtc catattttgt attttgattt tcatggtatt gagtattttg gctgtttagg
     5161 agagccgccc ataagcatga gcctcgagcg cctctccctg tactaagcct gaatcaaaaa
     5221 caatagatgc ttaaacctga atgttcttgt ttgtacttaa ggttttgcca agatctgccg
     5281 gtgaagttac cgaggaactt cctccattgc cctcgccggt gccgaagcaa caagaacagc
     5341 aacaggtgca gcaacacatt ccgggaaggt tgcgttcggt cagcatcaga ggagcggaac
     5401 gatcttccac taccttcatg gtcatttcaa gtgttttcat gatgtataaa atttatgtga
     5461 taaggcctac tctttattgg cttttcggct atctccagga ttaatccaat gcaaattaaa
     5521 atcaaataaa tcattaatca aaatataata gtgaatttaa cacaacaaaa taaattattt
     5581 aacattaaac atatgggcac aaaaaaaccg gagctataaa ctccggttca tatattagta
     5641 aataacctat tacgcattga taaataccct attacgcagt aagctttgtc aaaatctccg
     5701 tcaagccctg aggatcggga gagtaatagt cggcgccgat ttgtttgcga aactcatcgt
     5761 ttaccggggc tcctcccaca aaggtggtgg tttggggcga ggcctgcttg atggctttgt
     5821 ttatttcttc catactggcc atggtggtgg tcaatagggc agacatgccc acataggcgc
     5881 ctggatgggc ctggatagct tcgataaatt tctcggccgg aacatcgact cccaggtcga
     5941 tcacctcgaa gccgctgccc tcgaccatca tgcttaccaa attcttaccg atatcgtgca
     6001 ggtcgccttt taccgtacct atgataaatt tacccttgcg ctttacggcg ccggagttga
     6061 aatagggctt caggtggttc atggcggtac tcatagctct ggcagacatc agcacctggg
     6121 gcacaaacac tttgttttcc ctgaatttaa ctccgatttt tcccataccc actatcaatc
     6181 ctttggtcag gacgtcgctt ggagaaactc cctgctccaa agcccgtttg gtgtattcgt
     6241 cggcgccctc ctggcccctc atgtcgggag gatagggcga ggctaaattt attttaccgt
     6301 attcaacgca ataagcgatt ttgtctaaaa gttgttccat gttgttatta ttaaaataag
     6361 gtccgaccac agactgcaaa ttaaatcaat tttcgggggc tttacaaacg atattcccgg
     6421 cgcaattgtt gggctgtgga aaatatggtg tgaattttat cgtccgttac cttatcgtcc
     6481 atattgatgc agcagatacc cgtcagccaa ggattggcgc aatcgttgac cagtctgcgg
     6541 atatgtgctt cgagggtttc gcagctcatg gtattgatgc ttaccggatc gagccggata
     6601 ttgataaagg tagagggcaa atgtttacgc agacagacca tatctccacc ccagccggca
     6661 tccaaaaagt cgagatgagg aatttttgcc atctgggccg cgtgcctgtg gggatcggga
     6721 ccgcaataat gcacaccgta gggacggtat ttcttgctcc attccacatc gaagggaagc
     6781 agaaattctt cgtattgttc ctccgaaatc atggtatgcg agcattccga atgcaggtaa
     6841 acggctggtg tgatgttgat cacgttccgg tttaccgaaa tggaattact gccggtttct
     6901 tgttggataa agcagaaaaa acgctcaatc agctctgcga tttttttgaa atacagtttc
     6961 acttcgtcgg gttgcagcaa gaagtccata agtatttctt cgccccttaa atccatagcc
     7021 aggtttaaga tgcctcccca attcacatct cctttgagat atccgtaacg gcttttgagt
     7081 tccgacatca gtttttcgag tctcttaaag gccggaccgg caaaagcagc atcgacatct
     7141 acatgaaaat ctgctttttg agcgggaatt acctggggtg cgctgtcggc aaaatagcga
     7201 atttcacagc ccagcatttc agaaagtaaa taaccggctg cgttgtgaac ggctcctatt
     7261 tgcggtaaat ctttgtcgtg atcgcttcca agtccgtatt gcccgaagcg ttcgtacaat
     7321 tccttttcca tgcggcgctc cgattctact cttttaagcg gatggtaaaa gaagtcttcg
     7381 tcaaaatcga tgccgcaatg cttatgccac caggagggat gaaaaacgat ttccaccggt
     7441 aacggttcgt tgctgaaagc agggttcttg atataagtca tggctttagt tttaatggct
     7501 tcaaatgtac tgtttttttg ccggttcgaa ttttttgccg ttccgaaagt ccggccggct
     7561 ttgtattatc ttatctgaat ctgcaggctg cccttttgcg gcccgctcag ccatttggct
     7621 tgcgaggagg ggccaaccag atcggtcgaa ttgactttat tgcgcacacc tggtataacg
     7681 tcgagcacag caattccact ttgaggaatg gtgtacagca gggcgtcgcg tccgtcgcgg
     7741 ggcgtataaa cgcccaggaa gctgcccggc gtgccgtttc ctaaccagag ctgagcctga
     7801 gcggtttgga aatctaccca tttccagtcg ttgtaatagc ctttgaattc gggataaata
     7861 aaactttcgc ccggaatggg gtcgttgtat tctttgtccc acacatcgaa aagggtgccg
     7921 tgcatacggt tttgccacga gcgatagggg cctttgccca acagccgcat gccctgcatg
     7981 ttatcttcgg gataatcgaa gcaaatgcct gccagctcaa ccactccttc gtattgatat
     8041 tcgtaatcga cggtgatttc ttcccggtcg gtaatggtcc agcaaacgct gttcaggttg
     8101 ccgaagtatt gggttttgaa cagtattttg tcttcttgtt cttctacttc gaaagcgatc
     8161 agcctggagt tgccggagat atcggcgtaa attctgtctt tgcttttagc tgctttgtcg
     8221 tcgtggttgt aaaacacgtc catagagcgg tcgctgcgac gtgcggcgat aaaacgcggc
     8281 ccgcccgaaa aggacagatt attgtttcct ttactgattt gaatcaggta acccgtttcc
     8341 gtatcgaatt gtaaacgcgt ggttcccgat tctatggtca gggtatttcc ttcgcggctt
     8401 tggcttaatt tggcaggaga ggtgttggct tgtccggcta aagcagcccg ggtgtaatcc
     8461 cgttcgcttt tccagtacca gccccaggtg taaatttctt cgccccgggg attgagcacg
     8521 cttacataca aaacatcggc ctctttccaa ttggctggca gtttaagctg aaattcgccg
     8581 ctttgatgcg gggccagttt gggcccggct tgttcgccct ggcctataat gttgtgacct
     8641 ccgcttttat cgcccggttt ggcaaaattg gccaaagacc attttatgcg gcaatcttcg
     8701 agggaaataa aatcgtagcg gttttctaca tggaattttc ccacaaaagc ttccggatcg
     8761 gcaataaagc tgttttgatc ctgcctcagg gcttccgtcc gctccgtgca ggtgacggtt
     8821 ctgcccagag ccttgatttg taccggacac cagacttctt tgacggtaaa gaaactacct
     8881 tctttttcgt gatgaggccc cacgatgccg tcggcggcgt agttacccac attgtcgatg
     8941 cggccgttct ggtcggtgcg catcaggccc tcgtcggcaa acacccagag aaaggctccg
     9001 gcgcagcggg gatgtcggcg catcattacc cagtaatccc aaagtccggc gccgtggccg
     9061 ccgtcgtaga ggccgtgcag gatttcggtg ggcataaaga tttcgggttt gcgcatgtat
     9121 tcctgcgatt cgccataaga acggtaatgc atggtttcga agccgccgaa attaccctgg
     9181 gggtgaagta ccgggcgttt ttgcggatcc cagggatgaa attcaccgtc gagttccgtg
     9241 ttccagcccc cctcattgcc gttggaccac cagatgatgc tcggatggtt caggtcgcgg
     9301 gtaaccatct cttttaccag ttgtttcccc acttcggtgt cgtacttgcc gtgccagccg
     9361 ccgagttcgt tcattacgta aagtcccagc tcatcgcagg catccagaaa agcaggatcg
     9421 gggggataat gcgacaaacg gacggcgttc aggttcattt cgcgcataag acgcacatcg
     9481 ttgtaattgt cctctctgct cagggttctg ccggtttcgg ggcggaaact gtggcgatta
     9541 acgcccttga tcattacttt ttgaccgttg atgtataaac cgtcgctctc tttgacttcg
     9601 atggttctga atcctatcct ttgttcttta actatatgaa cggcctcctt gcccttgagc
     9661 aggctgaatt gcacatcgta taaatggggt gtttcggccg tccaggtttt tatgtccgtg
     9721 aattgccctt tcacaatgac cttgtccgaa cccggcctca attcggcttt cagggctttg
     9781 ccgatgtttt tgccttcccg gtcgagcaat tgtgtttcga cgctgtagtc gccctccagg
     9841 gcagtgctca aaaatacttc ggcgtaaaaa gagccgtcgg ctttggcgtc aatggctgta
     9901 cgatcgatgc tttgcgcagg cagggcctgt ataaataccg gacggaagat gcctccgaaa
     9961 ttccagtagt cggccctgcg ttcagccagg ttgacgctgc tgttttcgga ttctttgctc
    10021 acggtgactt cgagcaggtt ttccttgtcg ccaaagaata cccggtcgct gacatcggca
    10081 taaaaacgat aaaaagctcc ctgatgtagt ttgacggcac gtcgtttgtt tattt
//
LOCUS       DAOSGL010000049        10084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_5685_length_10084_cov_3.982052, whole genome shotgun sequence.
ACCESSION   DAOSGL010000049 DAOSGL010000000
VERSION     DAOSGL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5685_length_10084_cov_3.982052"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /gene="asnA"
                     /locus_tag="PKY68_02715"
     CDS             <1..825
                     /gene="asnA"
                     /locus_tag="PKY68_02715"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOH23984.1"
                     /translation="DLQDRKAEIVHSLAKWKRLTLADYEIERGYGIYTDMNAIRPDEE
                     LDNLHSIYVDQWDWEVTIGKQDRTPAYLKAVVQRIYSVLLQTEYMVYEQFPWIKPSLP
                     AEIHFIHAEELRQKYSDLDPKERENRICKEYGAVFVVGIGADLGDGQKHDMRAPDYDD
                     WSSEGENGLPGLNGDILLWNPLLGCAFELSSMGIRVDKKALLRQLKLSGTEERKSLYF
                     HQKLINDKLPLSIGGGIGQSRLCMFLLRKAHVGEIQASIWPEDMIERCAAHGIPLI"
     gene            896..1564
                     /gene="ung"
                     /locus_tag="PKY68_02720"
     CDS             896..1564
                     /gene="ung"
                     /locus_tag="PKY68_02720"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOH23985.1"
                     /translation="MVDVRIEQSWKSKLAKEFEQDYFAGLTDFVREEYRQYTIYPPGR
                     LIFNAFDLCPFEQVRVVILGQDPYHEPGQAHGLCFSVQEGVPLPPSLENIFREIRQDL
                     GEEPLPSGNLERWARQGVLLLNATLTVRAHRAGSHQNKGWERFTDAVVHRLAEEKQGL
                     VFLLWGAYAQRKGEFIDSNRHLVLKSVHPSPLSAYRGFFGNHHFSKTNQYLQSQGLDP
                     IDWR"
     gene            complement(1767..4730)
                     /locus_tag="PKY68_02725"
     CDS             complement(1767..4730)
                     /locus_tag="PKY68_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOH23986.1"
                     /translation="MMDVQPDYKLNTLKQLKNPVRQFNAVRLKSRFFGGFFGSLSGCT
                     LAILLLFSVPFVQNARGQSRVGETLTRDSVAATYSEETSLSDSLDRSAPETPTGVSET
                     VLSGENDTIPALSSDSLQVVPDSLANEEEKGGGGLEGINAVVEYQAEDSLIFEAGGMG
                     RLYKQGQIVYEDLNLEADYIQMSMDSSLIYASGIEDSTGTVIGSPVFKDTSGEYISKN
                     LKYNFNTRRGIINQVVTQQGEGYVVSGLTKKMEDDALYMVDGKYTTCDDHDNPHFYLQ
                     LSKAKVRPKKNIVAGPAHLVVEDVHLPLFIPFGFFPFTESYSSGIITPTYGDELSRGF
                     YLKDGGYYFAISDYIDLALTGELYSKGSWGGRLRTNYRKRYKFSGALNASYLVSVTSE
                     RNLPDYAQSKNLSLTWSHSQDPKANPFRSFSASVNFATSGYSRNDIASYYNPEVFSTN
                     TKSSSVNLTQRFPNFPLVLSASMSVSQRSKDSVLTVSLPNLTASVSRFYPFKRKNPVG
                     RERFYEKISMSYTGSLANSISEVKEYDFFKKSLVRDWRNGMRHSIPVSATFNLLNYIN
                     ITPSFSYTELWYSSSIDKYYDPVLNQLLTDTLWGFNRVWDYSASVSASTKLYGFFQPL
                     PKLFGTKVNMIRHVLTPSVSLGYRPDFGEERYGYYKTIDYIDVEGIEKTHTYSRYQNS
                     LYGTPGRGKSGTISFNVANNLEMKVRNDKDTINLFKKVSLIDNFSFGTSYNLAADSLN
                     WSNISANIRIKFGDLYTLNLSGSFDPYTYILNSAGNPVRVNVTQFEKYGIPGRLVGTG
                     TSFSYSINNDTFKKKDKEGKVKGRGGLSGSGSEPPDLDAGLSGPGDETEAFEEPEVIP
                     DPEAALYQPFKMPWNVSLSYTLRYARANFNKERLEYDMKLMHNLSFNGNLAITDKWRF
                     TLASSYNFNTKQLSTVNCTVSRDLHCWQMSASFIPVGRFKSYNFSIRVKSGMLQDLKY
                     EQQQNPRDNVVWGIR"
     gene            complement(4776..5312)
                     /locus_tag="PKY68_02730"
     CDS             complement(4776..5312)
                     /locus_tag="PKY68_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOH23987.1"
                     /translation="MEALQLLHRYLDPYPGLFDIVYSHGRSVSNKALEIMDLHPEIQA
                     DRKFVEEAALLHDIGVYKTYAPEIACFGSLPYICHGYLGADLLREEGYGRHALVCERH
                     TGAGISLRDIVDKELPLPRRDMLPVSIEEQLICFADKFFSKTELLKERSVAEARHKLE
                     RFGAEGLERFDFWCKSFL"
     gene            5298..6590
                     /locus_tag="PKY68_02735"
     CDS             5298..6590
                     /locus_tag="PKY68_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOH23988.1"
                     /translation="MQCFHKYTEIGDPASGPGRLQGKEKELFLYAQLSGTPMTKITLF
                     AVCLVTCLCSCFGSCSPGRGKAPASPLRTGADQTELYFPLLQDKRFALVLNQSSLIDK
                     TSLADSLCRSGLRPAFLFAPEHGFRGEAQAGETIRDGVDSLTNLTVYSLYGQQKKPSA
                     ELMQKLDLVVFDIQDVGTRFYTYLSTLHYLMEACAESGVELVVLDRPNPNDTIDGPLL
                     HEGYTSFVGMHSIPLLHGCTLGELAMMINSEGWLPNGLHCELRVIPVAGWRHGQAYSL
                     PIRPSPNLRDQQAVCLYPSLCLFEGSLMSVGRGTATPFKVVGYPDPRFGEFIFTPSGK
                     GSLYRDQTCYGLDLSGVNCVGGLNLEYVLSMYRRSGMGADFFTHARFFDLLAGNSSLR
                     EQILAGWNQAEIRAGWQEELVSYRKIRSKYLLYPDYQE"
     gene            6663..7784
                     /locus_tag="PKY68_02740"
     CDS             6663..7784
                     /locus_tag="PKY68_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH23989.1"
                     /translation="MYFRLLFLALFLATSLHQNLSGAEKPFTVVIDPGHGGRDPGAVG
                     ATAFEKNIVLSVALKLGKLIETNNKDINLIYTRNTDVFVKLEERSRIANKAKANLFIS
                     IHTDGVPNKKVSGASVFTLGQDKLSENIEVAKRENSVILLEENYEQRYEGFNPTSAES
                     YIMFEFLQDAHMDQSIAFASLVQNGFVRKKRTDRGVRQAPYWVLWDTGMPSVLIELGF
                     ITNPDEERYLKSEKGQDELVRCIYDAVVEFKNDYQRKSMAKTSEENSDTAGAIAQNTQ
                     EAREVQDVQAVQDVPVYKIQLFATSTLLDENNSEFLGLKNIDYYREKDLYKYTYGAFS
                     TEKEAAAERKKIASLFPDAFIVVFKDNIRISLEEARSKP"
     gene            7863..8762
                     /locus_tag="PKY68_02745"
     CDS             7863..8762
                     /locus_tag="PKY68_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOH23990.1"
                     /translation="MKKFKLNKESRIGLTVIIAIALFVWGLNYLKGINLLKQAQRYYV
                     VYNQIDGLVTSAPVMLDGFQVGLVKSIDYQYNSPGHILVELNLEKQLNLPVGTKAVLE
                     PALVGNPNISLILGPGPRLLAVGDTLTADRKADLMIMLEEELIPGIKDLIQRAGRVVS
                     QLEAQLDEGGDLNKSLASVRKTSERLASASASLDLLLANEIPSTIGNINSLSSNFNAL
                     GRELRTVDFAGISHRVDSTLLILQDVSLRLTRPDNSLGLLLNDTELYFNLNKTASSAN
                     ELLLDLRERPKRYVHFSLFGSKK"
     gene            9171..>10084
                     /locus_tag="PKY68_02750"
     CDS             9171..>10084
                     /locus_tag="PKY68_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOH23991.1"
                     /translation="MDFILNHEELWSKCLDIVRDIVPESNFSTWLLPIVPLKYQNEDL
                     LIQVPTHFFFEHIESNFSDVIGKSLQRITGKPTRLLYKIKLSDNFIDITPDQHPNKEK
                     AIQNLPPNSKAALVSPFEQLLPNRLDPQLNTSFHFGNFIAGNSNKLSRTAGVSIAENP
                     GKTIFNPLFVYGKSGVGKTHLLHAIGNHALYKNPELRVLYVSSHLFQVQYSQAYLNNS
                     INDFIHFYQSIDLLLIDDIHELSGKQGTQNVFFHIFNHLHQNSKQLVLTCDRAPKDLQ
                     GVEDRLLTRFKWGLTAEITQPDYQLRLD"
BASE COUNT         2724 a         2377 c         2423 g         2560 t
ORIGIN      
        1 gacctgcagg accgaaaagc cgaaatcgtg cattccctgg ccaaatggaa acgcctcacc
       61 ctggccgact acgaaattga aagaggttac ggcatttaca ccgatatgaa cgccatccgc
      121 ccggacgaag agttggacaa tttgcattcc atctacgtgg atcagtggga ctgggaagtc
      181 accataggca aacaagaccg taccccggct tatttgaaag ccgtagtaca acgtatatat
      241 tcggtgctgc tgcagaccga atacatggtc tatgaacaat tcccctggat caaaccctct
      301 ttgccggcag aaattcattt tattcatgcc gaagaattac gccaaaaata ctccgacctg
      361 gatcctaaag aacgtgaaaa ccgcatctgt aaagagtacg gggccgtctt tgtggtaggt
      421 atcggagccg atctgggcga cggacaaaag cacgatatgc gggcccccga ctacgacgac
      481 tggagcagcg agggggaaaa cggcctgccc ggtcttaacg gagatatttt actttggaat
      541 cccttgcttg gttgcgcttt tgaattgtct tccatgggga ttcgtgtcga caaaaaagcc
      601 ttgctgcgac agcttaaact gagcgggacc gaagagagaa aaagcctgta tttccaccaa
      661 aaactaatca acgacaagct tcccctgagc ataggcggcg gtatagggca atcgaggctc
      721 tgcatgtttt tgttgcgcaa agcccacgtg ggagaaattc aggccagcat ctggcccgaa
      781 gatatgatag aacgttgtgc cgcacacggc atcccactga tataaatagg gttgttaatc
      841 acagaaaagg aaagggcaaa gtaagagaat gccgttcctc tcattatgaa caaggatggt
      901 agatgtaaga atcgaacaat cgtggaaatc aaaactggcc aaagaattcg aacaagatta
      961 ttttgccggt ctgaccgatt ttgtacggga ggaatacaga caatatacca tttatccgcc
     1021 gggccggctg atttttaatg cctttgatct ctgccctttt gaacaggtca gggtggtgat
     1081 tttaggtcag gatccctatc atgagccggg acaagctcac ggactttgct tctcggtgca
     1141 ggaaggtgtg cccttgccac cttcgctcga aaatatcttt cgcgaaattc gtcaggatct
     1201 gggcgaagag cctctaccct cgggcaatct ggaacgctgg gcccgccagg gcgtactgct
     1261 gctcaatgcc acccttaccg tacgcgctca ccgggcaggc tctcatcaaa acaaaggctg
     1321 ggaacgcttt accgatgcgg tggtacaccg cctggccgaa gaaaagcagg gtctggtgtt
     1381 tttactttgg ggtgcttatg cccagcgcaa aggcgaattc atcgacagta accgccatct
     1441 ggtgcttaaa tcggtgcatc cctcgcccct gtcggcctac aggggtttct ttgggaatca
     1501 tcatttcagc aagaccaatc aatacttgca aagccagggt ctggatccta tagattggcg
     1561 ctaaagggaa gcaagagata cgcgagcttg gctatgctaa aagtctggat gctttaaatt
     1621 gacagcagag ggatgaaact tccagccttt agcgggccct ctgtgcaaac gcgaaagcac
     1681 ccatccttag aaggggcttc cttttttcaa agagaaaaat tcgtcgttgc cggaaaagaa
     1741 agcggccgga aaccaaaaca agttgtttag cgaatacccc agaccacatt gtcgcgggga
     1801 ttttgttgct gctcgtactt aaggtcttgc agcatgcccg atttaacacg gatggagaag
     1861 ttgtaagatt tgaaacggcc tacaggaata aaactggccg acatttgcca gcaatgcagg
     1921 tcgcgcgaaa cggtgcagtt taccgtagac aattgcttgg tattgaagtt gtaactgctg
     1981 gctaatgtaa atctccattt atcggtgatt gcgaggttgc cattgaaact caggttgtgc
     2041 atcagcttca tgtcgtactc cagacgctct ttgttaaagt tcgcacgggc ataacgcaag
     2101 gtataactca ggctgacgtt ccaaggcatt ttaaagggct gataaagggc ggcttcaggg
     2161 tcgggaataa cctcgggttc ctcaaaagct tcggtttcgt cgcccggacc cgacagcccg
     2221 gcatctaaat cagggggttc gcttccggaa cccgataaac ctcctctccc cttaaccttt
     2281 ccctctttgt cttttttctt gaaagtatcg ttgttgatgg agtaggaaaa cgatgtcccg
     2341 gtaccgacga gccggccggg aattccatat ttctcgaact gggtgacgtt gacccgtacc
     2401 ggattaccgg cactgttcag aatataggtg tagggatcga aagaaccgct cagattcaat
     2461 gtgtacaaat cgccgaactt gatacggata ttggccgaaa tattgctcca attcaagctg
     2521 tcggccgcca ggttgtagga ggtgccgaaa gagaaattat cgatcaggct caccttctta
     2581 aataaattta tcgtgtcttt gtcgtttctg actttcattt cgaggttatt ggccacatta
     2641 aaactgatcg tacccgattt gcccctgccg ggagtgccat acagagaatt ctggtaacgc
     2701 gaataggtat gggtcttttc gatgccctcg acatcgatat agtctatcgt tttgtaatag
     2761 ccgtatcgtt cctctccgaa atcgggcctg taaccgagcg aaacactggg ggtaagcacg
     2821 tgccggatca tattaacctt agtcccgaac agtttgggca aaggttgaaa aaagccgtat
     2881 aatttggtgg aggcactgac actggcagaa taatcccaga cccggttaaa gccccagagg
     2941 gtgtcggtaa gcaattgatt cagaaccgga tcgtagtatt tgtcgatgga gctggaatac
     3001 cagagttcgg tataggaaaa cgaaggagtg atattgatgt aattcaacag attaaacgtg
     3061 gccgaaacag ggatgctgtg ccgcatgccg ttgcgccagt cgcgtaccag cgattttttg
     3121 aagaaatcgt attctttcac ttcgcttatg ctgttggcca aagacccggt atagctcatg
     3181 ctgatttttt cgtaaaaacg ctccctgccc accggattct tgcgtttgaa gggatagaat
     3241 cgggaaacac tggccgtaag attgggcagc gaaacggtca gcaccgagtc tttgctgcgc
     3301 tgtgaaacgc tcatgctggc actcaggaca agcgggaaat taggaaaacg ttgggtcagg
     3361 ttgacactgg agcttttggt gttggtcgaa aaaacttcgg gattgtaata cgaagcgatg
     3421 tcgttgcggc tgtaaccact ggtggcaaaa ttgacactgg ccgaaaaact gcgaaaggga
     3481 ttggccttgg gatcctgcga atggctccag gtgagactca ggtttttgga ctgggcgtaa
     3541 tcgggcaggt tcctttcgct ggtgacactc accaagtaag aagcgttgag ggctcccgag
     3601 aacttgtaac gtttacggta attggttctg agccgccccc cccaggatcc tttggaataa
     3661 agctcgcctg tcagtgccaa atcgatgtaa tcgctgatgg caaaataata gccgccgtct
     3721 ttgagataaa aaccccggga aagctcatca ccgtaagtgg gcgttataat acccgatgag
     3781 taggattcgg taaaggggaa aaaaccgaaa ggaataaaca ggggcaaatg tacgtcttcg
     3841 accaccaaat gggcaggacc ggccacgatg ttctttttgg gacgcacctt ggctttgctc
     3901 agttgcagat aaaagtgcgg attgtcgtga tcatcgcagg tagtatattt gccgtcgacc
     3961 atgtagaggg catcgtcttc catcttctta gtcaggccgc ttacgacgta accttctccc
     4021 tgctgggtaa ccacctggtt tataatgccc cgccgggtgt tgaaattgta tttgaggttt
     4081 ttggaaatgt actcgccgct ggtatctttg aaaaccggac ttcctattac ggttccggtt
     4141 gaatcttcta ttccgctggc atagatcaag ctgctgtcca tgctcatttg aatatagtcg
     4201 gcttcgagat tgaggtcttc gtaaacaatc tgcccctgct tgtacaagcg ccccattcct
     4261 ccggcttcga aaatcaggga gtcttcggcc tggtattcta ctacggcgtt gatgccttcc
     4321 aggcctccgc cccctttttc ttcctcattc gccaggctgt cgggaacgac ctgaagactg
     4381 tctgaagaaa gcgccggaat agtatcgttt tcgccggaga ggacggtttc gcttacacct
     4441 gtgggagttt ccggggcaga acggtcaagg ctatcggaaa gcgaagtttc ttcggaataa
     4501 gtcgctgcaa cagagtcccg ggtaagcgtt tctccaacac gactctgtcc acgagcattc
     4561 tgaacgaaag gaactgaaaa taataaaagt atcgctaggg tacacccgga taaggagcca
     4621 aaaaagcctc cgaagaatct gcttttgagc cgaaccgcgt taaattgccg aacgggattc
     4681 ttgagctgct tcaatgtatt taacttataa tcaggttgta catccatcat actaccccag
     4741 ggatcaaagg gacaaaagta ggtaaaacca gcggattaga gaaagctctt gcaccaaaaa
     4801 tcgaaacgtt ccagcccttc ggcaccgaat cgctccagtt tatgccgtgc ttctgcgaca
     4861 ctgcgttctt ttaacaactc ggttttagaa aaaaacttat cggcaaaaca gatcaattgc
     4921 tcctcgatac tgacaggcaa catatcgcga cggggcaagg gcaattcttt gtctacgatg
     4981 tctcgaaggc tgatacctgc acccgtgtgc cgttcgcaaa ccaaagcatg ccgcccgtaa
     5041 ccctcctctc tgaggagatc ggctcccaag taaccgtgac aaatataagg tagagaaccg
     5101 aagcaagcta tctcgggggc ataagtcttg tacacaccga tatcgtgcag cagggctgct
     5161 tcttcgacaa actttctgtc ggcctgtatt tcgggatgta agtccattat ttccaaggcc
     5221 ttgttggaga cagaccggcc gtgggaataa actatatcaa aaagcccggg gtaaggatca
     5281 agatatcgat gtaacaattg cagtgcttcc ataaatatac ggagattggc gacccggctt
     5341 cgggccccgg gcgcttgcag ggcaaagaaa aagaattatt tttgtacgca caactatccg
     5401 gcacgcccat gaccaaaatt acgctgtttg cagtttgcct cgttacttgt ctttgttctt
     5461 gtttcggttc ctgctccccc ggccggggaa aagctccggc ctccccttta agaacaggcg
     5521 cagatcaaac cgagctttat ttcccattgt tacaagacaa acgttttgcc cttgtattga
     5581 accaaagctc cctgatcgac aagaccagct tggccgacag tctttgccgg tccggcttga
     5641 gaccggcttt tttatttgct cccgaacacg gcttcagagg cgaggcccaa gccggcgaaa
     5701 ctattcggga cggagtggac agtctgacga atctaacagt ctattctctc tatggccaac
     5761 agaaaaagcc aagtgcggag ctgatgcaaa aactcgattt ggttgttttt gatattcagg
     5821 atgtaggaac ccgtttttat acttacctga gcaccctgca ctacctgatg gaagcctgcg
     5881 cagaatccgg tgtggaactc gtcgtgctcg accggcccaa tcccaacgac accatcgacg
     5941 gacccttgtt gcacgaagga tatacctcct ttgtgggcat gcattccatt cctttattac
     6001 acggttgcac gctgggcgaa ctggccatga tgatcaattc agaaggctgg ctgccaaacg
     6061 gcctgcactg cgaacttcgg gttattcccg tagcaggctg gcgtcacgga caagcctact
     6121 ccctgcccat cagaccatcg cccaatcttc gcgaccaaca ggctgtttgc ctttatcctt
     6181 cgctttgttt gttcgaagga agcctgatga gcgtaggcag aggtaccgcc acccctttca
     6241 aagtggtggg ataccccgat ccccgctttg gagaatttat ttttactcct tccgggaagg
     6301 ggagtttata tcgggatcaa acctgttacg ggctggattt gagtggggta aactgtgtcg
     6361 ggggcttgaa tctggagtat gtattgagta tgtatcgccg ttcgggcatg ggggctgact
     6421 tttttaccca tgcacgattt tttgatttat tggccggcaa ctcatccttg agagagcaaa
     6481 tcctggcagg atggaatcag gcggaaattc gggccggctg gcaagaggag ttggtatctt
     6541 atcgcaagat acggtcgaag tatcttttat atccggatta tcaggaataa attcatactt
     6601 gctgcatcag ggtttatttt ctatttttgc gcttgttgta aattcagcta aacattgaat
     6661 ctatgtactt tcgtctcctg tttttggctc tgttccttgc cacttcgttg catcagaact
     6721 tatcgggagc agaaaaacct tttacggtag tcatcgatcc gggtcacggg ggcagagacc
     6781 cgggagcagt cggagccacc gccttcgaaa aaaacatagt gctttcggta gctttgaaac
     6841 tgggaaagct catagaaacc aacaacaaag acatcaacct gatttacacc cgtaacaccg
     6901 acgttttcgt taaactcgaa gaaagatcca gaatagccaa caaagccaaa gccaatctat
     6961 ttatttcgat tcataccgac ggtgtaccca acaaaaaggt ctcgggagcc tctgtattta
     7021 ccctgggaca ggataaactc agcgaaaaca tcgaagtggc caaacgcgaa aacagtgtta
     7081 tactgctcga agaaaattac gaacaacgtt acgaaggttt caatcctact tcggcagaat
     7141 cttacattat gttcgaattt ttgcaggatg ctcacatgga tcagagtatt gcttttgctt
     7201 cgcttgtaca aaacggtttt gtacgtaaaa aacgcactga caggggcgtg cgccaggccc
     7261 cctattgggt actctgggac accggcatgc catcggtact gatagagctg ggctttatta
     7321 ccaatcccga cgaagaacgc tacctgaaaa gcgagaaagg acaggatgaa ttggtgcgtt
     7381 gtatctacga tgccgtggtt gagttcaaaa acgattacca acggaaatcg atggccaaaa
     7441 cctctgagga aaattcggat acagcgggag caattgccca gaatacacaa gaagcacgag
     7501 aggttcagga tgttcaagct gtgcaggatg ttccggtata caaaatacag ctctttgcca
     7561 caagcacatt attagacgaa aacaacagtg aattcctggg actgaagaac atcgattatt
     7621 atcgggaaaa ggatttgtat aaatacacct acggcgcttt ctctacagaa aaggaagcgg
     7681 ccgccgaacg caaaaagatt gcctcgctgt ttcccgacgc tttcatagtc gtgtttaagg
     7741 acaacatccg tatcagtctg gaagaagcca gatcaaaacc gtaatacatt ttcaagaaag
     7801 taagcggtct ttagctacaa agttaccggc tgaaaaccca cgttaaaagt aataaggtta
     7861 ttatgaagaa atttaaactt aacaaggaaa gccgtatcgg gttgaccgtc attatcgcca
     7921 tagccttgtt tgtttggggg ctgaattacc tcaaaggtat caatctgctc aaacaagctc
     7981 agcggtatta tgttgtatac aatcaaattg acggcctggt tacctcggca ccggtgatgc
     8041 tggacggttt tcaggtagga ctggttaaaa gcattgatta tcagtataac tctcccggtc
     8101 atattttggt tgagctaaac ctcgaaaaac aactcaactt gcccgtgggc actaaggcgg
     8161 ttttggagcc tgccctggtg ggcaacccca acatcagcct gattctgggt ccgggtcctc
     8221 ggttgctggc cgtaggtgat accttgacgg ccgaccgcaa ggccgacctg atgattatgc
     8281 tggaagaaga gcttataccg ggcataaaag atttgattca acgagcaggc agagtcgttt
     8341 cccaactcga agctcaactg gacgaaggag gcgacctaaa caagagcctg gcttcggtgc
     8401 gcaagacgag cgaaaggttg gcctccgcat cagccagtct tgatttgttg ctggccaacg
     8461 aaattccctc tactatcgga aacatcaatt ccctgagcag taatttcaat gccttaggcc
     8521 gggaactcag aactgttgat tttgccggca tcagccatcg cgtcgacagc accttgctga
     8581 tattacaaga cgttagtctg cgcctgacca ggccggacaa cagcctgggc ttattactga
     8641 acgatacaga attgtacttt aacttaaaca aaacagcctc aagcgctaac gaactcctgc
     8701 tggatttaag agagcgaccg aaacgttacg ttcatttttc gctctttggt tccaaaaaat
     8761 agcaggatgc agaaaaatca ggtgtagaaa aaaaaaagaa ttttaagcct gtcaaaaaat
     8821 agccccaatg ttttataagg aattttcgga ctgttttcag aattaaatag caggttttaa
     8881 aacttaatgt aaaaataata ctggtctgtt atcccctttg ccctaaagcg attggaagtt
     8941 ttactgccaa tttaacaatc caagctgtca aaattacaaa gccctattta taagattgtt
     9001 gtgatttaaa tttttacaca agaatctgat tatcaaacta ttaatcttaa ccgattaaaa
     9061 ctaaaacaga tacaaacttg caaaactttt aaacaaaatc ttcgtttgtt ttattcgttt
     9121 tttcttcaaa aatttgcagt acgtttcaag ctaaactcag aggaacaatt atggatttta
     9181 ttttaaacca tgaagagttg tggagcaaat gcctcgacat agtcagggat attgttcctg
     9241 aatccaactt ttctacctgg ctgctcccta ttgttcctct aaagtatcaa aacgaggatt
     9301 tactcataca ggttcccacg catttctttt ttgagcacat cgaaagcaat ttttcggatg
     9361 taatcggtaa aagcctgcaa cgtattacag gcaaacccac ccggctgctg tacaaaatta
     9421 aattgtcgga caacttcatc gatatcacgc ccgatcaaca ccccaataaa gaaaaggcta
     9481 tccagaatct gccgcctaac agcaaagctg ccctggtaag ccctttcgag caattgttac
     9541 ctaacaggct tgatcctcag ctaaacacga gttttcattt cggaaacttc attgccggca
     9601 acagtaataa actttcgcgt acggctggcg tttcgattgc cgaaaacccg ggcaaaacca
     9661 ttttcaatcc actgtttgtt tatggtaagt ccggtgtggg taaaactcat cttttgcacg
     9721 ctataggcaa tcatgcgctc tataaaaatc ccgagctgag agtgctttat gtctcctcgc
     9781 atttgtttca ggtgcagtat tcacaggctt atctcaacaa cagcatcaac gactttatcc
     9841 atttttacca gagcatcgac ttactgttga tagacgatat tcatgagttg tcgggtaaac
     9901 agggaacgca aaatgtgttt ttccatattt tcaaccacct gcaccaaaac agcaagcaac
     9961 tggtgcttac ctgcgacagg gctcccaaag atttgcaggg agtggaagat cgtctgctga
    10021 ccaggtttaa atggggactt acggccgaaa taacccagcc cgattaccaa ttaaggctgg
    10081 acat
//
LOCUS       DAOSGL010000050         9603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6050_length_9603_cov_4.329179, whole genome shotgun sequence.
ACCESSION   DAOSGL010000050 DAOSGL010000000
VERSION     DAOSGL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6050_length_9603_cov_4.329179"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKY68_02755"
     CDS             <1..385
                     /locus_tag="PKY68_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOH23992.1"
                     /translation="GRDGTVGNQLLSRFTLFIDVGGRQLHLHPNSHFKKKFSYNVAGL
                     ELIQSIRGLPRYEVAGVWPDSPAAQAGILPGDVIRQVEGDNAYAMSLSELRGYFEQSR
                     KRTLRVVLERDGKLLTLNLDLKSKI"
     gene            651..3662
                     /locus_tag="PKY68_02760"
     CDS             651..3662
                     /locus_tag="PKY68_02760"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOH23993.1"
                     /translation="MRKTAFCLVAVLLLYGKVSIETSALQAASPAAPAVRTTSVAPAA
                     PATPAARTTSVAPAAPAGSAVATTSIAPAAAATEWPAVRPEYKPGTRWWWLGSAVDKE
                     NLDWNLHYLHQAGIGSVEITPIYGVQGEEARDIPYLSPRWMEMLKHVQDQAARLEMIV
                     DMNGGTGWPFGGPEIDPAYAASRQLIQRYELEGISVSPSAGSKSAPTQSINLQVKDNR
                     QRPHAQLQALLFVSADGSREILPIEGVKDNILELPAGKKGELIALYCGKTLQQVKRSA
                     PGGEGLVMNHLSKEALDHYLKKFDRAFEQSDAAWPSSFFNDSYEVYGADWSEQLLEEF
                     MKRRSYDLRLYLPELLGEGDSEVIARVVCDYRETISDMLLENFTIPWTEWAHERGSQT
                     RNQAHGSPGNLLDLYAAMDIPECESFGCTNFDLPNLRVDEEIRRNDGNPATLKYASSA
                     AHVSGKNYTSSESMTWLTEHFRTSLALIKPEMDQLFLNGINRVFYHGTPYTPKEAAWP
                     GWLFYASILVNPNNTIFRDMPALNDYITRVQSFMQEGKPDNELLLYLPIYDIWQNYRQ
                     SNYLTFVIHSISDKLPEFNGLVYKLREAGYDMDYISDRQLLETGFADGSLQTPGAQYK
                     AILVPHCRVIPPATLRHLLNLAHQGANVMFLGSLPQEVPGLHAAAERREELRQLWKET
                     GLKNDPKNQQSVTYGQGMLTVASDLTELMKVSGIEPEEMRSVQGLDYIRRRYEDGYVY
                     FVAMQHNRLVDAWVPLAKPASSVMFFDPLTGQKAPAPLRRGQRDQAEVYLQIKPGQSL
                     IVRTFDQGNLPGATYPIYEPSTLNYGCRKEQKNQEALPLLGNWSFEFTEGQPLIPGQF
                     KMKGQPQAWTELQVEGADTYAGTGVYKLEFKLPKADADDWLLDFGSLAESARIRVNGK
                     DAGMVWCVPYEIRLGEFLIPGKKNTIEIAVTNMPANLIADYDRRGVNWRIFKDINIVS
                     VFYKPITFDVWPVQPSGLLQTPRLIPLINKK"
     gene            3715..5679
                     /locus_tag="PKY68_02765"
     CDS             3715..5679
                     /locus_tag="PKY68_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH23994.1"
                     /translation="MKKIFLIVAVSLFLPALQAQNDLVEWKVQTNKTVSDINPDMYGI
                     FFEDINFGADGGLYAELIKNRSFEFPQNLMGWKTFGKVELRSIDAPFERNPHYVRLSP
                     SGHGHKHTGLENEGFRGIGLKEGATYRFSVWARSIDGRSQTIRIEFIDAQNNIMDRRN
                     LSIDSREWQKYSIEITASRTEPKALLRIFLDSRSRGSVDLEHISLFPKDTWMDRENGL
                     RKDLAQALADLKPGVFRFPGGCIVEGTDLDTRYNWKNSVGPVENRPLNENRWHYTFTN
                     RFFPDYFQSYGLGFFEFFQLAEDMGAEPLPVLSVGLACQFQNNDMSAHVPVEELDPYV
                     QDALDLIEFANGPADSEWGKLRADMGHPESFNMKYLGIGNEQWGPEFPERLEVFVKAI
                     RQAYPDIQIIGSSGPSAEGEQFDYLWPEMKRLKVDLVDEHYYKDPNWFFANASRYDKY
                     DRKGPKVFAGEYASHDHPRGKANNFRSALSEACFMTGLERNADVVRLATYAPLFAHVD
                     AWQWNPDLIWFDNLRSVKTPNWYVQQLYGHYKGTGVLRSASGKEALSGQNNLYASAVR
                     DAGKKEIILKIGNSSNQPQRMKIELSGLKKFNPDAKWITMQANDLNVVNTLDEPFNIV
                     PGEASFKLTGNVFEFEVKAQSFNVLVIAEE"
     gene            complement(5682..7079)
                     /locus_tag="PKY68_02770"
     CDS             complement(5682..7079)
                     /locus_tag="PKY68_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23995.1"
                     /translation="MKRLLLFCVVLLTLSGCRNPQAGLLFTKEAPDIFPDYQFVHIPW
                     NIAPLNFIINNQGNKAIVLIEGPETTLRVKSDRKVQFPLRKWKKFLLKNKGKTLEVKL
                     RLKQKKSWVEYPAFEWYVTREAVDPYLVYQRIRPTSTVWANQGIYQRNLENFDEDCLL
                     DNSFTDADGLNTFAFCAGRPDRLLFHKRRPSAQTYLLVNGEKTELSQQSFNLMAHPSW
                     HPSGQFVAFAINHSDLPFYGNIGLPAQLYNPASDILVYDVVNKQLITGSELISKDSYE
                     TFPAFSADGRSLYFCTATQTESTQKGDISYDLCRLDFYPEDRRFGSRVDTLVRAERIG
                     RSISFPKPSPDGRFILLTLHSGGRFNLWGQDSDLFYYLLKDELHGVKSCYASNSQYAE
                     DYASWSSNGAWFVFGSRRLNGLSSQLYLSYIDEKERPRKAVLLPIKDPDSYRNPDQIS
                     CHLPELLLRKAFRME"
     gene            7212..9362
                     /locus_tag="PKY68_02775"
     CDS             7212..9362
                     /locus_tag="PKY68_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOH23996.1"
                     /translation="MKKIIFNRQTLPYLITCFFVLSPVLQVQAKSRKTVQNDFNTPLH
                     LLQADYKFPYGIPQQKDIQSVLERVLVFLEESTPTQVLDGKTQQVLSDYSRIDADARL
                     QQGAFRLASYEWGVTYMGMLSAGQALSNQRFTDYTLDRFRFLAETAPYFRQQMEAGRP
                     MDSQMRQMLQPAALDDCGSMCAAMIKAAPLMPDTDLKPLINNYVDYIMRKQSRLSDGT
                     LSRNRPFFNSVWLDDLFMSLPALVQMGRYGSDRRYYDEACRQFKLFAEKMFVPGQGLF
                     RHAWVQGMDSHPDFFWGRANGWALMTLVEILEVLPESHPQRAYLLDMLRAQIKALAAL
                     QSPEGLWHQLLDRNDTYLETSASAIFCYAFAKAINRGWVDAQAYGPAVLLAWNAVAAK
                     VDAQGMVQGTCVGTGMAFDPAFYAHRPVSPYAAHGYGPVLLAGAEVLRLLENFHPKMN
                     DSAVQFYTHHFNTTAPIFEERIENNHGLKVPGYSRRGRRPVVFTIGDSTVRNSDGSGR
                     NGQWGWGNFLGDFLNPDSFSVENHALGGRSSRTFIEEGLWQRVLDVVEEGDYVLMQFG
                     HNDGGEPHRGRARATLKGNGDQAESFVMESTGDTILVHTYGWYMRRYIQDVLDKKAVP
                     VVCSLIPRNIWQDGKVGRNDDTYALWAQQAAESIEGAYFIDLNKLICDKYDVLGKNFV
                     NTLYYGDHTHTSELGAKMNAAIVAEEFKRLIQEK"
     gene            9372..>9603
                     /locus_tag="PKY68_02780"
     CDS             9372..>9603
                     /locus_tag="PKY68_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH23997.1"
                     /translation="MNRLKRMLGLLMLALVCSSLQAERLTISMDKNWEFCLKDLPADS
                     VFIVQQGWRQLDLPHDWSIEGKYDKDHPSGRGG"
BASE COUNT         2474 a         2305 c         2437 g         2387 t
ORIGIN      
        1 gggcagagac ggcaccgtcg gcaatcagtt gttgagccgc ttcactcttt ttatcgatgt
       61 cgggggcaga caactgcatt tacaccccaa cagccatttt aaaaagaaat tcagctacaa
      121 tgtggccgga ctggagctta ttcagagcat cagaggtttg cccaggtatg aggttgccgg
      181 ggtgtggccc gattccccgg cggcacaagc agggatcttg ccgggcgatg ttattcgcca
      241 ggtagaaggc gataatgctt atgccatgag tttgtcggaa ttgagaggct attttgaaca
      301 aagtagaaaa agaactctgc gagttgtgtt ggaacgagat ggtaagctgc tcacgctgaa
      361 tttggaccta aagtcgaaaa tttaaagttc aggcttcatt gtgggaagtc tgttattaag
      421 caatattttc ctgagagaag catgttatcg agcaatgcgt tctgtttctt gagcagaatt
      481 attctgtaaa attttttgat ctcagagatt ataacccaat ttcccgggtt gcgtggccgc
      541 agttgtgaat ttaaatgtaa aaatatcaaa aagctcctgg actgacagta gaaaagctta
      601 tttttgtaaa agtacatttt taccctccgt ggtaagtaaa gaggaatatc atgagaaaaa
      661 cagctttttg cttagtggcc gtactgttgt tgtacggcaa ggtttctatt gaaacaagtg
      721 ccctgcaggc agcttctccg gctgctcccg cggtgagaac cacatctgtt gcaccggctg
      781 caccggctac tcccgcggcg cggaccacat ctgtcgcacc ggctgctccg gccggttctg
      841 ctgtcgcgac cacctccatt gcgcctgctg ctgcggccac agaatggccc gcagttcgtc
      901 ccgaatataa gcccggaacc cgttggtggt ggttgggcag cgccgtggac aaggagaatc
      961 tggactggaa tttgcactat ttgcatcagg caggtatagg cagtgtggaa atcaccccca
     1021 tttatggtgt acagggcgaa gaagcccgcg atattcccta tctgtcgccc cgctggatgg
     1081 agatgctcaa gcatgtgcaa gaccaggccg cccgcctgga gatgatagtg gatatgaacg
     1141 gaggtaccgg ctggcccttc ggagggcccg aaatagatcc tgcttatgcg gccagtcgtc
     1201 agctgataca gcgttacgag ctcgaaggta tatccgtttc tccttctgcc ggttccaaat
     1261 ctgctcccac acaaagcatt aatctccaag tgaaggacaa ccggcagcgg cctcatgctc
     1321 aattgcaggc attgttgttt gtgtcggccg acggtagtcg agaaattctg cccatagagg
     1381 gagtaaaaga taatatactg gaattgcccg ccgggaaaaa aggcgaactc attgcccttt
     1441 attgcggcaa gaccctgcaa caggtcaagc gttcggctcc cggaggagaa ggcctggtca
     1501 tgaatcacct gtcgaaagag gctctcgatc attacttgaa gaagtttgac cgggcttttg
     1561 aacaatccga tgccgcttgg cccagcagtt tctttaacga ttcctacgaa gtgtacgggg
     1621 ccgactggtc ggaacaatta ctggaggaat ttatgaagcg aagatcctac gatttacgct
     1681 tatatctgcc ggaattgctg ggcgaaggcg actctgaagt tattgccagg gtcgtttgcg
     1741 actacaggga gactatttcg gatatgttgc tggaaaattt cactattccc tggacggaat
     1801 gggctcatga acgcggcagt caaacccgga atcaggccca cggttcaccg ggaaatctgc
     1861 tggatctcta tgccgccatg gatattcccg aatgcgagtc tttcggttgt accaactttg
     1921 acctgcccaa tttgcgggta gacgaagaga tacgccgtaa cgacggcaat ccggccactc
     1981 tcaaatacgc ttcatctgcc gcccatgtca gcgggaaaaa ctacacttca tccgaaagta
     2041 tgacctggct gaccgaacat ttcagaactt cgctggccct gatcaagccc gaaatggacc
     2101 aattgtttct gaatggtatc aaccgggtgt tttaccacgg cacgccatat acgcccaaag
     2161 aggcggcctg gccgggctgg ctgttttatg cttccatact ggtgaacccc aacaatacca
     2221 ttttcaggga tatgccggct ttgaacgact acataaccag agttcaatcc tttatgcaag
     2281 aaggcaaacc cgacaatgaa cttttgttgt atttacccat ttacgacatt tggcaaaact
     2341 accgtcagtc aaattaccta accttcgtta ttcacagtat ttccgacaaa ctgcccgaat
     2401 tcaacggctt ggtttataag ctgcgggagg ccggttacga tatggattat atttccgaca
     2461 gacaattgtt ggagacagga tttgccgatg gcagcctgca aacgcccggt gcgcaatata
     2521 aggcaatatt ggtgccgcat tgtcgggtaa tacctccggc caccttacgt catttgctca
     2581 atttagccca ccagggagct aatgttatgt tcttgggcag tttgcctcaa gaagtgccgg
     2641 gtctgcacgc cgccgctgaa aggcgagagg aacttcgtca attatggaaa gaaaccggcc
     2701 tgaaaaacga tccgaaaaat cagcagtcgg tgacttacgg tcagggaatg ctgacggtgg
     2761 cttcagattt gacagagctt atgaaagttt ccggtatcga gcccgaagaa atgagatccg
     2821 ttcaggggtt ggattatatc agacgccgtt acgaagacgg ttatgtttat tttgtggcca
     2881 tgcagcacaa tcgcctggtc gatgcctggg tgcccctggc caagcccgcc tcttcggtga
     2941 tgttttttga tcctctgacc ggccaaaaag ccccggctcc cctgcgcagg ggccagagag
     3001 atcaggccga ggtttatttg caaatcaaac ccggacaatc cctgatcgtc cgtacttttg
     3061 atcaaggcaa cttgccggga gcaacctatc ccatttacga gccaagtacg ctcaattacg
     3121 gttgccggaa agaacaaaaa aatcaggagg cgctgcccct gctgggcaac tggagctttg
     3181 aatttacaga gggccagcct cttattcccg gacaattcaa aatgaaaggc cagcctcaag
     3241 cctggacaga actgcaggtg gaaggagccg acacctatgc cggtaccggg gtttataaac
     3301 tggaattcaa gttacccaaa gcggacgccg acgactggtt gctcgatttc ggctccttag
     3361 ccgaaagtgc aaggatacgc gtgaacggca aagatgccgg aatggtgtgg tgcgtgccct
     3421 acgagatcag actgggcgag tttctgattc ccggcaagaa aaacaccatc gaaattgccg
     3481 ttaccaacat gcctgccaat cttattgcag attacgaccg gcggggtgta aactggcgca
     3541 tattcaaaga catcaacata gtgagcgtat tttacaagcc catcactttc gatgtatggc
     3601 ctgtgcagcc ttcgggatta ctccagaccc ctcgattgat tcctttaata aataaaaaat
     3661 agctttatct ttgacgtgaa gtaaaaattg tataattcgt ttaatcgttt ctttatgaaa
     3721 aaaatttttt tgatagtggc cgtttcgctg ttcttaccgg cgctacaggc acagaatgat
     3781 cttgtagagt ggaaagtgca gaccaacaag acggtttccg atattaaccc cgacatgtac
     3841 ggcattttct tcgaagacat caactttgga gcagacgggg gcttgtatgc cgaattgata
     3901 aaaaaccgtt ccttcgaatt tcctcaaaac cttatgggtt ggaaaacctt tggcaaagtg
     3961 gagctgagga gcatcgatgc tccctttgag cgcaatcctc attatgtacg tttgtctcct
     4021 tcggggcacg gacacaaaca taccgggcta gaaaacgagg gtttcagggg tataggcctc
     4081 aaagaaggcg ccacctatcg cttttcggtt tgggccagat caatcgacgg aagatctcag
     4141 accatacgca ttgaatttat tgatgcacaa aacaacatta tggacagaag gaatctgagc
     4201 atcgattcca gagaatggca aaagtattcg atagaaatca ccgcttcgag aactgaaccc
     4261 aaagctctgc ttcgcatttt tctcgattcc cgtagccggg gctctgtaga cctggaacat
     4321 atttcgcttt tccccaaaga cacctggatg gacagggaaa acggcttgcg caaagacctg
     4381 gctcaggctt tggccgatct taaacccggc gttttccgtt tccccggagg ttgcatagtc
     4441 gaaggcaccg atctggatac ccgttacaat tggaaaaact cggtaggacc ggtcgaaaat
     4501 cgtcccctga acgaaaaccg ctggcattat acgtttacca atcgcttttt cccggattat
     4561 ttccaatcct acggtctggg cttttttgaa ttctttcaac tggccgaaga tatgggtgca
     4621 gaaccgctgc cggtattgag cgtaggcctg gcctgtcagt tccagaacaa cgatatgagc
     4681 gctcatgtgc ccgtagaaga gttagaccct tatgtgcagg atgccctcga cctgattgaa
     4741 tttgccaacg gcccggccga cagcgaatgg ggtaaattaa gggccgatat gggacatccc
     4801 gaatccttca atatgaagta tttgggcata ggcaacgagc aatggggacc ggaatttccc
     4861 gaaagactcg aagtctttgt aaaagccata cgtcaggcat atcccgatat tcagatcata
     4921 ggtagctcgg gtccctctgc cgagggtgaa caattcgact acttatggcc cgaaatgaaa
     4981 cgcctcaaag ttgatttggt agatgagcat tattacaaag accccaattg gttttttgcc
     5041 aatgcttccc gttacgataa atacgaccgc aaaggtccca aagtttttgc cggcgaatat
     5101 gcctcgcacg atcatccccg gggcaaagcc aacaattttc gctccgcctt gtccgaagcc
     5161 tgttttatga ccggactgga acgcaatgcc gatgtggtca gactggccac ttatgctccc
     5221 ctgtttgctc atgtggatgc ctggcaatgg aatcccgatt tgatctggtt cgataattta
     5281 cgcagcgtta aaactcccaa ctggtatgtg caacaattat acggccacta caaaggcacc
     5341 ggagtcttgc gctctgccag cggcaaggaa gccttgagcg gccaaaacaa cctttatgcc
     5401 agtgctgtca gagacgccgg taaaaaagaa attatactta agatcggtaa tagtagcaat
     5461 cagccgcagc gcatgaagat tgaactgtcc ggcctgaaaa aattcaatcc cgacgccaag
     5521 tggattacga tgcaagccaa cgacctgaat gtggttaata ctttggacga gcccttcaat
     5581 atcgtaccgg gcgaagccag ctttaagctg acaggaaatg ttttcgagtt tgaagtcaaa
     5641 gcccaatcct tcaatgtgct ggtgatagct gaagaataga tttattccat acggaaagct
     5701 tttcggagca ggagttccgg aaggtgacaa gagatttgat ccgggttccg gtaggagtcc
     5761 ggatctttta tgggcaataa tactgcctta cgaggtcgtt ctttttcgtc tatatagctg
     5821 agatacaact gagaactcag gccattcagc ctgcggctgc caaatacaaa ccaggctccg
     5881 ttggacgacc aggaagcata atcctcggcg tactggctgt tgctggcgta gcaggatttt
     5941 actccgtgca gttcgtcctt caacaaataa taaaacaaat cactgtcttg cccccagaga
     6001 ttgaaacggc cgccggaatg cagggtcagc aagataaaac gtccgtcggg agaaggcttg
     6061 gggaaggaaa tacttctgcc tatgcgttcg gccctgacca gggtatctac ccggctgcca
     6121 aagcgcctgt cttcgggata gaaatccaga cggcagaggt cgtaactaat gtctcccttt
     6181 tgtgttgatt ccgtttgggt tgccgtacaa aaatacaggc tcctgccgtc ggccgaaaat
     6241 gccggaaaag tctcgtaact atccttggaa atcagctcag atccggtgat taactgtttg
     6301 tttaccacat cataaactaa aatatcggaa gcaggattgt acagttgtgc gggcaaaccg
     6361 atgtttccgt aaaagggaag atcggaatga ttgatggcaa aggccacaaa ctggccggag
     6421 ggatgccagg acggatgggc catgaggttg aagctttgtt gactgagctc tgttttttct
     6481 ccattcacca acaagtaagt ttgtgccgag ggccttcgtt tatgaaaaag cagtcgatcg
     6541 ggacgaccgg cacagaaggc aaaagtattc aggccgtcgg cgtcggtaaa actgttgtcc
     6601 aacaggcaat cttcgtcgaa attctccaga ttgcgttggt aaatgccctg attggcccac
     6661 acagtcgagg tgggccgtat gcgttgatac accaggtaag gatctacagc ctcccgggtg
     6721 acataccatt caaaagcagg gtattctacc cagcttttct tctgtttcaa gcgtagtttt
     6781 acctccaggg tttttccttt gtttttcaac aggaattttt tccattttcg caggggaaat
     6841 tgcactttcc tgtcgctctt tacccgaagc gttgtctccg gtccttcgat caggacaatc
     6901 gctttgttgc cctggttatt gataataaaa ttcagcggag caatattcca gggaatatgt
     6961 acaaattgat aatcggggaa aatatccgga gcctccttgg tgaataacag gccggcttgt
     7021 gggttacgac aaccggataa cgtcaataat acaacgcaaa acaggagaag tcttttcata
     7081 gacaggagct agagaactca aaagtactca aaattgccgc ttagaggctc agttttaaca
     7141 aataaaatat caaattaagc atacatctac gcgccggata agctattttt gtaaacatca
     7201 agaaacacgc catgaaaaaa atcattttta acaggcagac tttgccttat cttatcactt
     7261 gtttttttgt attgagcccg gtgcttcaag ttcaggctaa aagtcgcaaa acagtccaga
     7321 acgacttcaa cactcctttg catttattgc aagccgatta caagtttcct tacggcattc
     7381 cgcaacagaa agacattcag tcggtgctgg aacgcgtact cgtatttctc gaagaatcca
     7441 caccgacgca agtgctggat ggcaaaactc agcaggtcct gagcgattac agccggattg
     7501 atgcggacgc caggctgcag cagggcgcct ttcgattggc cagttacgaa tggggagtca
     7561 cttatatggg tatgttgtcg gccggacaag ccttgagtaa tcagcgtttt actgattata
     7621 cgctggatcg tttccgtttt ctggccgaaa cggctcctta ttttaggcaa cagatggaag
     7681 ccggccggcc gatggacagc cagatgagac aaatgcttca gcccgccgcg ctcgacgatt
     7741 gcggctccat gtgtgctgcc atgataaagg cagctccact tatgccggat accgacctga
     7801 agcctttgat aaacaactac gtcgattata tcatgcgcaa gcagtcccgt ctatccgacg
     7861 gaaccttgtc gcgcaaccgc ccttttttca attcagtctg gctcgacgat ttgtttatga
     7921 gtctgcctgc tttggtgcaa atgggcagat acggctccga caggcgttat tacgacgagg
     7981 cttgccgtca gtttaaactt tttgccgaaa aaatgtttgt gcccgggcaa ggtttattca
     8041 ggcatgcctg ggtacaaggc atggacagtc atcccgattt tttttggggc agagccaacg
     8101 gctgggcttt gatgactctg gtcgagatac tggaggtttt gcctgaatca caccctcagc
     8161 gggcctatct tttggatatg ctcagagctc agatcaaggc cttggcggca ctgcagtctc
     8221 ccgaaggttt gtggcatcaa ctgctcgaca ggaacgatac ttatctggag acttcggctt
     8281 cggccatatt ttgttatgcc tttgccaaag ccatcaaccg gggctgggta gacgcgcagg
     8341 cttacggccc ggccgttttg ctggcctgga atgccgtcgc agcaaaagtg gatgcgcagg
     8401 gcatggtgca gggcacctgc gttggaaccg gcatggcttt tgaccctgct ttttatgccc
     8461 acagacccgt cagtccctac gcggctcacg gttacggccc cgttttgttg gccggtgccg
     8521 aggtgttgag gctgttggag aactttcatc ccaaaatgaa cgattcggcc gtacagtttt
     8581 atactcatca cttcaacacc acggctccca ttttcgaaga aagaatcgaa aacaatcacg
     8641 gacttaaagt gcccggttac agccgcagag gccggcggcc ggtggttttt accattggtg
     8701 attccactgt gcgcaattcc gacggcagcg gccgcaacgg acaatggggt tggggcaact
     8761 ttttgggaga ctttttgaat cccgattcct tctcggtcga gaatcacgcc ctgggtggcc
     8821 gtagcagcag gacttttatt gaagaaggtc tatggcaaag agtgctcgat gtggtagagg
     8881 aaggagatta cgtactgatg caattcggac acaacgacgg gggtgaaccc caccgtggcc
     8941 gtgcccgggc caccctcaaa ggcaacggcg accaggctga gtcttttgtg atggaaagca
     9001 ccggcgatac cattttggta catacttacg gttggtatat gcgtcgttac attcaggatg
     9061 ttctcgacaa aaaggctgtt ccggtggttt gcagcctgat tccccgcaat atttggcaag
     9121 acggcaaagt gggccgtaac gatgatacct acgccctttg ggcccaacag gcagccgaga
     9181 gcatagaagg cgcctatttt atcgatttaa acaaattgat ttgcgacaag tacgacgttt
     9241 tgggcaaaaa ctttgtcaat actttgtatt acggagacca tacccatacc tccgagttgg
     9301 gagccaaaat gaacgctgcc attgtggcag aagaattcaa acgattaata caagaaaaat
     9361 aatacagaac aatgaacaga ttaaaacgaa tgcttgggtt gttgatgttg gcgcttgtat
     9421 gcagttcttt gcaagcggaa cgtttaacca tatccatgga caaaaactgg gaattttgcc
     9481 tgaaagatct gccggcagat tcggtattta ttgtgcaaca aggctggcgt caattggatt
     9541 taccacacga ctggagcatc gaaggcaaat acgacaaaga ccatcccagc ggcaggggag
     9601 gtg
//
LOCUS       DAOSGL010000051         9570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6078_length_9570_cov_5.024277, whole genome shotgun sequence.
ACCESSION   DAOSGL010000051 DAOSGL010000000
VERSION     DAOSGL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6078_length_9570_cov_5.024277"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..968
                     /locus_tag="PKY68_02785"
     CDS             30..968
                     /locus_tag="PKY68_02785"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOH23998.1"
                     /translation="MRLKGPIKIFSGTKSHYLAEKICAALECPLGRMNIQHFADGEFS
                     VSYEESIRGDYVFLVQSTFPNSDNLMELLLMIDAAKRASAGKIIAVVPYFGWARQDRK
                     DKPRVSIAAKLIADILSVAGIDRLITMDLHADQIQGFFDVPVDHLYASTIFLPYIRSL
                     NLDNLVIATPDVGGSKRANSYSKYLGVPMVLCHKSRNKPNEVAEMRIVGDVAGKNVLL
                     VDDMVDTAGTLTKAANLISSEGALSVRAVASHCVMSGKATELVENSELTEVIFTDSIP
                     YDKVSSKVKILSIADMFADTIQRVYDNRSISSQYLI"
     gene            complement(1009..2541)
                     /locus_tag="PKY68_02790"
     CDS             complement(1009..2541)
                     /locus_tag="PKY68_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH23999.1"
                     /translation="MKKLVFLIAAGLLFACNQADKSKRDFGTAQGDLGNGYFLNPILG
                     GDYPDPTIMREGKDYYMTHSSFDYVPGLVVFHSTDLVNWQPISYALKRYLGSVWAPDI
                     CKHEDRYYIYFTVAHPSGRMNFVTYADSPYGPWSDPIDLKVGNIDPGHAVGEDGSRWI
                     FLSGGQRARLNDDGLSIVPGSLEKVYEGWIYPSDWVTEGMCLEGPKIRKIGDYFYYLN
                     AEGGTAGPPTSHMVVAARSKSINGPWENSPYNPIVRTWQASERWWSKGHGSIIDSPKG
                     DWWIVYHAYENGFTSLGRQTLLEPIEWTDDGWFVAPSGTAIERPLKKPLSSGEKIDRR
                     SRLGEFRVGLDWKFYKDFDTSRFEVNQGELTLQAQGDSPGNSSPIMCVAGERTYEIEV
                     EIEKDEHATAGLVLYYNSNFYAGIGFDQQRTLRFRQGILRTASRHEGGNRLWLRLRND
                     HQVLTGAYSYDGINWIKETWGMEISGYNHNTLYEFQSMLPALYAAGEGKVKFRNFKYT
                     SL"
     gene            complement(2553..4097)
                     /locus_tag="PKY68_02795"
     CDS             complement(2553..4097)
                     /locus_tag="PKY68_02795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOH24000.1"
                     /translation="MAQQEDVFKKLISHCKEYGFVFPSSEIYDGLSAVYDYGQMGVEL
                     KNNLKKYWWDSMVLMHENIVGIDSAIFMHPTIWKASGHVDAFNDPLIDNRDSKKRYRA
                     DVLIEEQLAKYDDKIDKEVAKAAKRFGEAFDEALFRVTNPRVVENLQKKEVLHQRFAE
                     ALNQNDLEALRQIILDEEIVCPVSGSKNWTEVRQFNLMFSTEMGSTADGAMKVYLRPE
                     TAQGIFVNYLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVKP
                     GTELEWFEKWKEIRLKWHKALGLGDHKYRFHDHEKLAHYANAATDIEFQLPFGFKEVE
                     GIHSRTDFDLSNHEKYSGKSIKYFDPELNESYVPYVVETSIGLDRTFLSILAGSYEEE
                     MLPGGESRVLLHLPPALAPVKLAILPLVKKDGLPEKARQIIDELRFDFKCQYDEKDSI
                     GRRYRRQDAIGTPFCATVDHDTLQDNAVTVRDRDSMEQIRVPIEELRSLISDKVDLKQ
                     LLKKQA"
     gene            4207..6927
                     /locus_tag="PKY68_02800"
     CDS             4207..6927
                     /locus_tag="PKY68_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOH24001.1"
                     /translation="MEEEHLLNETDVPDNLDGRTRADKDKDKNTLSPDSLNPAHTKQQ
                     LTGMYRNWFLDYASYVILERAVPHIDDGLKPVQRRILHSMKRLDDARYNKVANIIGHT
                     MQFHPHGDASIGDALVQLGQKELLIDCQGNWGNLLTGDGAAAPRYIEARLSKFALEVL
                     FNPKITEWKPSYDGRNQEPVCLPVKFPLLLAQGAEGIAVGLSSKILPHNFNELIDASI
                     AYLRNEPFKLYPDFQTGGYIDVSRYNDGERGGSVKVRAKISKLDNKTLVITEIPFGKT
                     TSGIIDSILKANEKNKIKIRKVDDNTAQNAEILIHLASGTSSDKTIDALYAFSDCEVS
                     ISPNCCVIADEKPQFLTVSDVLRYSADNTKELLKKELLIRKGELLESLLFASLEKIFI
                     EERIYKDKAFEESTSMDKALSHIDKRLEPFKKDFVREVKRDDLLRLTEIKMARILKFN
                     IDKAEEAIVAMQEQIKEIDHHLATLVEYTIAWFESLKKKYGAAFPRKTEIRSFDTIEA
                     AEVVEATEKLYINREEGFIGTSLKKDELVCPCSNLDDVILFYKDGKYKVVKVSDKMFV
                     GKNVLYLNVFKKNDTRTIYNVIYRDGKFDNKGGGVSYIKRFAVSGVTRDKEYDLTKGS
                     PGSRIQYFSANPNGEAEIVRVTLKPAPRIRKLQFEKDFGEIAIKGRQSMGNILTKNDV
                     FKIALKQKGGSTLGGREVWFDPDVLRLNYDGRGEYLGEFHAEDSILVMTKSGEYYMTN
                     IDLSNHYEDNILRIEKFEEDKIWTAALFDAEQQNYPYLKRFPLEFSQRKLSFTGENPE
                     SRLILLSDTYYPRIEVSFGGDDSHREKLVLDCDEFIGTKSFKAKGKRITTYEVAEIKE
                     IEPLRFPPPKEDDNNSEPEAVIVSESDESEEDNNPGKLIDEITGQMTLFD"
     gene            6963..7044
                     /locus_tag="PKY68_02805"
     tRNA            6963..7044
                     /locus_tag="PKY68_02805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6997..6999,aa:Leu,seq:tag)
     gene            7301..7498
                     /locus_tag="PKY68_02810"
     CDS             7301..7498
                     /locus_tag="PKY68_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24002.1"
                     /translation="MNTIKGISGISGISGIIPGLTFGLVCSLALVGCKHSIGADNHFD
                     HTGASNDSIDIYPELHSSYTK"
     gene            7539..>9570
                     /locus_tag="PKY68_02815"
     CDS             7539..>9570
                     /locus_tag="PKY68_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH24003.1"
                     /translation="MKPTCRTLFFTLLLSIMASGPVYSGNWNHFNNNWKFTKGNPENA
                     QSINFDDSSWEKVNLPHDWAIKGPFNEQENGSTGKLPWRGEGWYRNTFTLSSEDQGKV
                     VYIKFDGVMSSPQIFVNGEPAGKWDYGYSSFYLDISDFVKYGQENGIAVYVDNRKHDS
                     RWYPGAGIYRKVEMMTTEKVHEEIWGTYITTPVVSRSYAEMRILSNVWNKDSEDKKVT
                     VEAVLVSPEGFEIKTVASRQRTLPAGSLFQFDNWMTVVRPQIWDVDHPVLYSIKTKVI
                     VDGKVVDSNETPFGFRSFEFTSDDGFHLNGRRLQLRGVNLHHDHGPLGAAFNKRSMER
                     KLEILKEMGCNAVRTSHNICAPELIELCDKMGLLVFNEAFDKYDKKADIFEATDFYEF
                     GERVIKNFVLRDRNNPSVFIWSVGNEMGDIQSNSNFGLQRLAAMVGFVRKYDITRPVT
                     MACDQESNAHWRHFDYYDVHAWNYGQRWETARKLDPSKSVIISESASTVSTRGFYELP
                     LPEKPTDFSQSNQVSSYDLHAPSWAEVCDDDFMWQENDKYIAGEFVWTGFDYLGEPTP
                     YYSSRSSYFGIVDLCGIPKDRYYLYQSYWRPEKDLIHILPHWNWPGHEGEKVPVFVYT
                     NGDEAELFLNGKSLGKQSKKPKSEISTERYRLMWHDVVYEAGELKAVAYRAGK"
BASE COUNT         2584 a         2177 c         2332 g         2477 t
ORIGIN      
        1 aacctcacca cttctcaagc ttaagatcta tgcgtttaaa ggggcctatc aaaatctttt
       61 caggaaccaa atcacattat ctggccgaaa aaatatgtgc ggccttggaa tgtccgctgg
      121 ggagaatgaa tatccagcat tttgccgacg gggaattttc cgtctcttac gaagaatcca
      181 tacgagggga ttacgtcttt ttggtgcagt ctacctttcc taattccgat aatttgatgg
      241 agttgctgct gatgattgat gcagccaagc gggcttcggc cgggaagata attgctgtag
      301 taccctattt tggctgggcc aggcaagatc gcaaggataa acccagggtg tcgattgctg
      361 ccaaactgat tgcagatatt ttgtctgtgg ccggtataga ccgtctgatc accatggatc
      421 ttcatgccga tcagattcag ggctttttcg atgtgcccgt agaccattta tacgcctcta
      481 ccatttttct tccctatatc cgctctctca atctggataa tttagttatt gccactccgg
      541 acgtgggagg ttccaaaaga gccaactcat attccaaata ccttggtgta cccatggtac
      601 tttgtcacaa atcgcgcaat aagcccaacg aagtggccga aatgcgcatt gtgggagatg
      661 tcgcaggcaa aaatgtcctt ttggtagacg atatggtcga taccgcaggt actcttacca
      721 aggcagccaa tctgatatcg agcgaaggtg ccctgtctgt gcgggcagtg gcaagtcatt
      781 gtgttatgtc gggcaaagcc accgagttgg tcgaaaattc agaactcaca gaagtgattt
      841 ttaccgacag cattccttac gataaagttt cgtccaaggt aaaaatactg agcattgccg
      901 atatgttcgc cgacaccatt caacgtgttt acgacaatcg ttccatctct tcgcaatacc
      961 tcatttaaca agaatcccca tcggggttgc tgttgctcaa aatgctgatc agagcgaagt
     1021 atacttgaaa tttctaaatt tgactttgcc ttcgccggca gcataaagag cgggcaacat
     1081 gctttggaat tcgtagagcg tgttgtggtt gtaaccggaa atctccatgc cccaagtctc
     1141 tttaatccag ttgattccgt cgtaactgta ggcgccggtc aacacctgat ggtcgtttct
     1201 gagacgcagc cacagacggt tgcctccctc gtggcgcgag gctgttcgca gtatgccctg
     1261 gcgaaagcgc aaggttcgct gttggtcaaa acctataccg gcatagaaat tgctgttgta
     1321 atacagaacc agcccggctg tggcgtgttc gtctttttct atttcaactt caatttcgta
     1381 agtgcgctcg cctgccacgc acatgatggg cgaggagttt ccggggctgt cgccttgagc
     1441 ctggagtgtc agctcccctt ggttcacttc aaaacgagaa gtatcgaaat ccttgtaaaa
     1501 cttccagtcc agaccaactc tgaattctcc cagtctgcta cggcgatcga ttttctctcc
     1561 ggaagacagg ggttttttaa gcggacgctc aatagccgtg ccgcttgggg ccacaaacca
     1621 tccgtcgtcg gtccattcga tcggttcaag cagggtttgc cgtcccaggg aggtgaaacc
     1681 attttcgtag gcatggtaaa caatccacca atcgcctttg ggggaatcta tgatcgatcc
     1741 gtgtcctttg gaccaccagc gttcggaggc ttgccaggtg cggacaatgg gattgtaggg
     1801 agagttttcc caagggccgt tgatcgattt tgagcgtgcg gcaaccacca tgtggctggt
     1861 aggcgggccg gcagtgccac cctcggcgtt gagataataa aaataatccc cgattttacg
     1921 aatcttgggg ccctccaggc acatgccttc ggtgacccaa tcggatggat aaatccaacc
     1981 ctcgtacact ttttccagac tgccgggaac aatcgaaaga ccatcgtcgt tgagtctggc
     2041 cctttgccct ccgctcagga agatccagcg cgatccgtct tcgccgacgg catgtccggg
     2101 atcgatattc cccactttta aatcgatggg atcgctccag ggaccgtagg gagaatcggc
     2161 gtaagtgaca aaattcattc tgcccgaagg atgggccaca gtaaaataga tgtaatagcg
     2221 atcttcgtgt ttgcagatat ccggagccca gactgagccc agatagcgct tcaaggcgta
     2281 actgattggt tgccagttga ccaggtcggt ggaatgaaat acaaccaggc cgggaacgta
     2341 gtcaaacgaa gaatgggtca tgtaataatc tttaccctcc ctcataatgg tcggatcggg
     2401 gtaatctccg cccaatatgg gattgagaaa atacccattg cccaggtctc cttgagcggt
     2461 tccgaaatcc cgtttgcttt tgtcggcctg attgcaagcg aaaagcaggc cggcggctat
     2521 cagaaatact aattttttca tggtaggttg gtttaagctt gttttttgag taattgtttc
     2581 agatcgactt tgtcggaaat cagcgatctc agctcttcga tgggaacccg aatttgttcc
     2641 atgctgtcgc gatcgcgcac cgtaacggcg ttgtcttgca gtgtgtcgtg gtcaaccgtg
     2701 gcgcaaaaag gcgttccgat ggcatcttgt ctgcggtagc gcctgccgat ggagtctttt
     2761 tcgtcgtatt ggcatttaaa atcgaagcga agctcatcga tgatctgacg ggctttttcg
     2821 ggcaggccgt cttttttgac caggggcagt atggccagtt tgacgggcgc cagggccgga
     2881 ggcaggtgga gcaacacccg gctttcgcct ccgggcagca tttcttcttc gtaagaaccg
     2941 gccagaatgc ttaggaaggt acggtcgagt ccgatagaag tttctacgac atagggcaca
     3001 taggattcgt tgagttccgg gtcgaaatac ttgatgcttt tgccggaata tttttcgtgg
     3061 ttgctcaggt cgaaatcggt tctggagtgg ataccctcca cttccttgaa gccgaaaggc
     3121 aactggaatt caatatcggt ggcggcgttg gcgtaatgtg ccagtttctc gtggtcgtga
     3181 aagcgatatt tatgatcgcc caggcccagg gctttgtgcc atttcaggcg gatttccttc
     3241 cacttttcaa accactcgag ttcggtgccg ggtttgacaa aaaactgcat ttccatttgt
     3301 tcaaactccc gcattctgaa aataaactgg cgggcaacaa tttcgttgcg aaaagcttta
     3361 ccgatctgag cgatgccaaa aggaattttc atccttccgg ttttttgaac gttgaggtaa
     3421 ttgacaaaaa taccctgagc agtttcgggc cttaaataga ccttcatggc gccgtcggca
     3481 gtggaaccca tttcggtgga aaacatcagg ttaaactgac gcacctccgt ccagtttttg
     3541 ctgccgctaa caggacatac aatttcttcg tccaatatta tctgacgcag tgcctccagg
     3601 tcgttttggt tgagagcttc ggcaaagcgc tgatgtagaa cttctttctt ttgcaggttt
     3661 tcgacaactc tgggattggt aactctaaac agggcttcgt caaaggcttc tccaaaacgt
     3721 ttggcagctt tggctacttc tttatcgatt ttatcgtcgt acttagccag ttgttcttcg
     3781 atgagcacat cggcgcggta gcgctttttg gaatccctgt tgtcgatgag tgggtcgttg
     3841 aatgcgtcta cgtgacccga agctttccag atagtcggat gcataaagat ggccgaatcg
     3901 ataccgacaa tgttttcgtg catcagcacc atagagtccc accaatattt tttcaggttg
     3961 tttttgagtt caactcccat ttgaccgtaa tcgtaaacgg ccgacaatcc gtcgtaaatt
     4021 tcgctggaag ggaaaacaaa accgtattct ttgcaatgtg aaatcagttt cttaaaaaca
     4081 tcttcttgtt gtgccatagc tgcctttttt tagtttgcaa aggtagggtt taatttgttt
     4141 ttatgcacat cggacgaaaa aaatcgtatc tttgcggctt aaaacaactc aacactatac
     4201 ccttatatgg aggaagaaca tttgctgaat gagactgatg ttccggacaa tttagatggt
     4261 cggaccagag cagataaaga taaagacaaa aatacgctct cgcctgactc gctcaaccct
     4321 gcccacacaa agcaacaatt aacaggaatg taccgcaatt ggttcctgga ttatgcttcg
     4381 tatgtgatcc ttgagcgtgc cgttccccat atcgacgatg gtctgaagcc tgtgcagcgt
     4441 agaattctgc actccatgaa acgtttggac gatgcccgtt acaacaaagt ggcgaacatt
     4501 attggccata ccatgcagtt tcatcctcac ggcgatgcct ccataggcga tgctttggtg
     4561 cagttggggc aaaaggagtt gttgatcgat tgccagggaa actggggcaa cctgcttacc
     4621 ggagacggag ctgcggctcc gcgttacata gaagccaggc tgtccaaatt tgcactggag
     4681 gtgctgttca accctaaaat taccgaatgg aaaccctcgt acgacggcag aaaccaggaa
     4741 ccggtttgtc tgccggtaaa attcccttta ttactggcac agggtgccga agggattgcg
     4801 gtaggccttt cctctaaaat tctccctcac aatttcaacg agttgataga cgcctccatt
     4861 gcttacttgc gcaatgagcc gtttaaactt tatccggatt ttcaaaccgg tggttacata
     4921 gatgtcagtc gctataacga cggagaacgc ggcggtagcg ttaaagtacg ggccaaaatc
     4981 agcaaactcg acaataaaac attggttatt accgagattc cttttggcaa aaccacctcg
     5041 ggcattatag actccatttt aaaagccaac gaaaaaaata aaatcaagat ccggaaagta
     5101 gacgataata ccgctcaaaa tgccgagatt ttgatacacc tggcttcggg cacatcttcg
     5161 gataaaacca ttgacgcact gtatgctttc agcgattgcg aggtaagcat ttctcccaat
     5221 tgctgtgtga ttgcagacga aaagccccaa ttcctgacgg taagcgatgt gttgaggtat
     5281 tcagccgaca acaccaagga attgctcaaa aaggagttgc ttatccgcaa gggtgaattg
     5341 ctcgaaagcc tcttgtttgc gtcccttgaa aagattttta tcgaagaaag gatctacaag
     5401 gacaaagctt tcgaagaaag taccagcatg gacaaggccc tgtcgcatat cgacaaaagg
     5461 ctggagccct tcaaaaagga ttttgtacgc gaggtcaaac gcgatgattt gctgcgattg
     5521 acggaaatta aaatggcccg tatcctgaaa ttcaacatcg acaaagccga agaagccata
     5581 gtagcgatgc aggaacagat caaggagatc gatcatcact tggccactct ggtcgaatac
     5641 accattgcct ggttcgaaag cctgaaaaag aaatacggtg ccgctttccc ccgcaaaacc
     5701 gagatccgga gtttcgatac catcgaagcg gccgaagtag tggaagccac cgaaaaatta
     5761 tacatcaacc gcgaagaagg ttttattggt acttccctca aaaaagacga attggtttgt
     5821 ccctgttcca atttggatga tgtcattctt ttttacaaag acggcaagta caaggtggtc
     5881 aaggtaagcg acaagatgtt tgtcggcaaa aacgtgcttt acctgaatgt ctttaagaaa
     5941 aacgataccc gcaccattta caatgttatt taccgggacg gcaaattcga caacaaggga
     6001 gggggcgttt cttatatcaa gcgttttgcc gtgagcggcg taactagaga taaggaatac
     6061 gatctgacca aaggcagccc gggttcgcgt attcagtatt tcagcgccaa tccgaatggt
     6121 gaggccgaaa ttgtccgtgt taccctcaag ccggctccca gaatcaggaa attgcaattc
     6181 gaaaaagatt ttggagagat agccatcaaa ggccgtcaat ccatgggaaa tattctgacc
     6241 aaaaacgatg tcttcaaaat tgcgctgaag caaaagggcg gctccacatt gggcggccgc
     6301 gaagtgtggt tcgatcccga tgtgctgaga ctgaattacg acggacgcgg cgaatacctg
     6361 ggtgagttcc acgccgaaga cagcattttg gttatgacaa aaagcggaga atactatatg
     6421 acgaatattg acctgagtaa tcattacgaa gacaatattt tgcgcataga aaagtttgag
     6481 gaggacaaaa tctggacggc ggcgcttttt gatgcagagc aacagaacta tccttatctg
     6541 aaacgtttcc cactcgagtt ttcgcaacgc aaactgagtt ttaccggaga gaatcccgaa
     6601 tcccggctaa tactgctcag cgatacttat tacccgcgca tcgaagtcag ctttggaggc
     6661 gacgacagtc atcgggaaaa actggtactg gattgcgatg aattcatagg aaccaagagt
     6721 ttcaaagcca aagggaaacg cataaccacc tacgaagttg ccgaaatcaa ggaaatagag
     6781 cctttacgtt ttccgcctcc caaggaagac gacaacaatt ccgaaccaga agccgtaatt
     6841 gtttctgagt ccgatgaatc tgaagaggac aacaatcccg gcaagctgat cgacgaaatt
     6901 accggacaaa tgactctttt tgactaataa aattattcag ctacctttgc gctgattttt
     6961 aggcggctgt ggtggaattg gcagtcacgt cagacttagg atctgatgcc gaaaggcgtg
     7021 ggggttcgag tcccttcagc cgcactttta caatttagga gaaaaccgag gcggttttct
     7081 cctaaatttg ttttggggca attattcttt atgtcgcttt ctccggtaaa atccaggtgt
     7141 tgatgccttc ttcaaaaaca aaccgaaacc catcttgttg tacaagaacc gttaaagttt
     7201 gcggttggtg ccttcctcaa aaacaggccg aaactcattc gaaatggagt agaattttgc
     7261 gataaaaata aaaagtgttt acttcacagc cagattgatt atgaatacaa taaaaggaat
     7321 atcaggaata tcaggaatat caggaataat accgggccta accttcgggc ttgtatgctc
     7381 tttagccctg gttggttgca aacacagtat aggagctgac aatcattttg accacacggg
     7441 ggcaagcaac gacagcatag atatttatcc ggaattgcac agcagctaca ccaagtaaat
     7501 tctataaaac aacgaaataa gcgttaaaac acatgtttat gaaaccaacc tgcagaactc
     7561 tcttctttac tctccttttg agcataatgg cgtccggccc cgtgtattcc ggcaattgga
     7621 accattttaa taacaactgg aaattcacca agggcaatcc cgaaaatgcc caatcgataa
     7681 actttgatga ttcttcgtgg gaaaaggtta atctgcccca cgactgggct ataaagggcc
     7741 cttttaacga acaggaaaac ggtagcaccg ggaaattgcc ctggagaggc gaaggctggt
     7801 atcgcaacac atttactctg agttccgaag accagggcaa agtggtatat ataaaattcg
     7861 acggagttat gtcgtctccg cagatatttg tgaacgggga acctgccggg aaatgggatt
     7921 atggctacag cagcttttac ctcgatattt cagactttgt aaaatacggg caggaaaacg
     7981 gcattgccgt gtatgttgac aacaggaagc acgatagtcg ctggtatccc ggagccggca
     8041 tttaccgtaa ggtggaaatg atgacaaccg aaaaagtaca tgaagaaatc tgggggacgt
     8101 atatcacaac acctgttgta agccggtctt atgcggaaat gcgtattttg tcgaatgtct
     8161 ggaacaagga ttccgaagac aaaaaagtta cggtggaagc cgtgcttgtc agtcccgaag
     8221 gatttgaaat taaaaccgtc gcttcgagac aaagaaccct acccgccgga agtctttttc
     8281 aatttgacaa ctggatgact gttgtccggc cgcaaatctg ggatgtggac catccggttt
     8341 tgtacagcat aaaaaccaag gtgatagtcg atggtaaagt ggtggattcc aacgaaacgc
     8401 cttttggttt cagaagcttt gaatttactt ccgacgatgg ttttcatctc aatggaagac
     8461 gattacaact caggggcgta aaccttcacc acgatcacgg tcccctggga gctgctttta
     8521 ataaacgttc catggaacgt aaactggaaa tcctgaaaga gatgggttgt aatgctgttc
     8581 gcaccagtca taatatttgc gctcccgagt tgattgaatt atgcgacaaa atgggactgc
     8641 tggtttttaa cgaagccttc gacaaatacg acaaaaaagc ggatattttt gaggctaccg
     8701 atttttatga atttggagaa cgggtaatca agaatttcgt gctgcgcgat aggaataatc
     8761 cttcggtgtt tatttggagt gtcggaaatg aaatgggcga tatacagagc aattccaatt
     8821 ttggcttaca gaggttggcc gctatggtgg gttttgtcag gaaatacgat attacgcgcc
     8881 cggtaaccat ggcttgtgac caggaaagca acgcacattg gcgtcatttc gattactacg
     8941 atgttcatgc ctggaattac ggccaacgtt gggaaacagc ccgaaaactc gatccttcca
     9001 aatcggtaat cataagtgag tcggcatcaa cagtcagtac aaggggcttt tatgagctgc
     9061 ctcttcccga aaaaccgacc gatttcagcc aatcaaacca ggtaagttct tacgatctgc
     9121 acgcaccctc ctgggccgaa gtctgcgacg atgattttat gtggcaggaa aacgataaat
     9181 acattgccgg tgaattcgta tggaccggtt tcgattattt gggtgaaccc actccttatt
     9241 atagctcgcg cagttcctat tttggtattg tcgatttgtg cggcattccc aaagaccggt
     9301 attacctgta ccaaagctat tggcggcccg aaaaagatct gatacatatt ttgccgcact
     9361 ggaattggcc aggacatgag ggtgaaaaag tgcccgtatt tgtttatacc aatggcgacg
     9421 aagcggaatt attcctgaac ggaaaatcgt tggggaaaca atccaaaaaa cccaaatcgg
     9481 agatttccac cgaaagatat cgtttgatgt ggcacgatgt ggtttacgaa gccggcgaac
     9541 tgaaagcagt ggcttaccgg gcggggaaag
//
LOCUS       DAOSGL010000052         9384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6229_length_9384_cov_3.760639, whole genome shotgun sequence.
ACCESSION   DAOSGL010000052 DAOSGL010000000
VERSION     DAOSGL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6229_length_9384_cov_3.760639"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PKY68_02820"
     CDS             complement(<1..1093)
                     /locus_tag="PKY68_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOH24004.1"
                     /translation="MKTRILSIAFLVLCYLPSTLSQVNISLTLDQETVPMSRDLIGVF
                     FEDINYAADGGLYAELVQNRSFEYYVVDGYTNLGPLTAWSGVQEGGASVALSVVKNHP
                     LNQHNTNCLKLVIYNQGRAAGVKNTGFDGIALKQNENYFFSAYLKRDTDHDKAVVVQL
                     KSSGGYIIASDTIPAIGSEWQQYHLTLKSKITFNDAALYILPQGSGNVYMDMVSLFPE
                     NTFKNRRNGLRADLAQAIADLQPRFVRFPGGCISHGKGLDNAYRWKHTVGDVAVRKPN
                     WNLWGYHQSYGLGFYEYFLFCEDLGAKPLPVLPVGVSCQFRNREVAPLEDMGPWIQDA
                     IDLVEFANGDTSTTWGALRAEMGHPEPFHM"
     gene            1391..2583
                     /locus_tag="PKY68_02825"
                     /pseudo
     CDS             1391..2583
                     /locus_tag="PKY68_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361026.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
     gene            2580..3395
                     /locus_tag="PKY68_02830"
     CDS             2580..3395
                     /locus_tag="PKY68_02830"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH24005.1"
                     /translation="MKKVILITGVSSGFGKEAAVLMAQAGYTVYGTVRKTVKPLQGVN
                     ILYMDLTQPASIDKAVAELLEREKRIDVLINNAGMHLGGPIEEAPSELYRRQLDTNLH
                     GMVHLLQAVLPTMRAQNKGLIINFSSIGGLMGLPFQAFYSASKFAIEGLSEALRMELR
                     AFGIKVVLVNPGDFHTSNTANRINVCDQKGPYARQFEISKARFESDETAGGQPAVMAR
                     KLLKIIAAKHPRHRYVVAAPDQKLAVLLKRILPSDWFMPILASYYGIKKRASN"
     gene            3543..>4745
                     /locus_tag="PKY68_02835"
     CDS             3543..>4745
                     /locus_tag="PKY68_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004384514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOH24006.1"
                     /translation="MKKTILCLIALLTISLLGAQEKTDAMLFGDVKTLDGKQHLSFIQ
                     IQVKGTNIGTLTDASGHFKIANLPLGKQIIVARGLGFITQEREVFMERGKAVTLYFEL
                     EEDAMNLEQVVITGTRSRHYIKDVPVRTEVITAKTIENKNAANLYRALEGIPGIRVEN
                     QCQACNFTMIRMQGLGAEHTQVLIDGQPLYSGLAGVYGLQQFSTVDVDKIEVIKGAGS
                     ALYGSGAVAGAINIISREPSYQPQTKIETQIGSHGTQRYDLSSSLRNEKGNVGLTIFA
                     QRYSEQAIDETGEGSTREEVRKPDGVSDHVDSELTNLGFSLFVNDAFGGSDQLVLRGR
                     SVFEQRQGGLLTDDYYTNPFTDGTENIVTKRYEASLSYQKKLGLRSEMNFNTAYVNHQ
                     RNATNDSYL"
     assembly_gap    4746..4755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4756..5972
                     /locus_tag="PKY68_02840"
     CDS             <4756..5972
                     /locus_tag="PKY68_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH24007.1"
                     /translation="YLDTHGDSVPDLRIMRPYLADEQTFTSTLSFSSALNTHRLVFGA
                     QFFYDHIDESGMYVVVDESSSYYGDSYRSMATKSAREVGLFAQDEWAIGRWMLVPGLR
                     LDHHRSGESYKADRQVSDLSLFPETSFEATTLNPRLAIRYKLSDRITLRANVGKGFRA
                     PYGFSEDLHLCSGSPRVWKSSGLEPETSVSYNLSADYYGRKIRLSANLFRTDLKNKIG
                     FSDADAQVAALGYDYQWKNIDDAFVQGLELTAVYSPVSQLHLSMDFSLNRGEYKQERE
                     DWKGTAYEKESRYISRFPSTAGNFMLEYAPANWALVLNSNYQGSMYIDYYNTDIDPLL
                     GDQSKIKKTERFVLLNAKVSRRFGQYKLYAGVDNILDYIQDERHLDDAAYLYAPICGV
                     VFYAGLSIDIRH"
     gene            6060..6734
                     /locus_tag="PKY68_02845"
     CDS             6060..6734
                     /locus_tag="PKY68_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24008.1"
                     /translation="MADLVIENPSLLLLLERFGMETALNDTDVAQLCEQYEVNTPVFL
                     LLCNLYNGFFPQRKDINPEQHRLTDIIKYIKNSHRFYTFEKYPQIRDYIAQLYERQCP
                     EDIQQLEIFFGDYFEEVQEHLRYEEEVVFPYCSYLLEGKAKGEINAFSSAEYRDHHTD
                     IETKLADLKNLMLKHLSLDGDFILKRKILYALMELENDLYIHSQVEELVLLPLLEHLE
                     FKDRNG"
     gene            6727..7332
                     /locus_tag="PKY68_02850"
     CDS             6727..7332
                     /locus_tag="PKY68_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOH24009.1"
                     /translation="MDKCRVNIAILEPSTVLYEGLSTILLKTHHHYYFFRFADLEELA
                     VMYKLTPFDIVVANPCMLQNKQKAFVRLKKKMPQVYWIALVYSFYDDELLSLFDNKFS
                     ITEDPKVMLNCFERACDSCKCNEKPGKKKLLSERETEVLIALTKGLANKEIAEMMNIS
                     IHTVMTHRKNIVEKTGLHSLPALTMYAISKKLIAFEPGGEP"
     gene            7416..8918
                     /locus_tag="PKY68_02855"
     CDS             7416..8918
                     /locus_tag="PKY68_02855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOH24010.1"
                     /translation="MALLKEEISRTFNEYLLIPGLTTKDCVPEKIDLRTPLVRYSKGQ
                     SSEIQLNIPFVSAIMQAVSDSNMAIALAKNGGLSFIFGSQPIEEQAEMVRKVKKHKAG
                     FVVSDANLRPDQRLQDVIDLKAKTGFSTIGITHDGSSNGKLLGIVTSRDYRPGKDDPS
                     KIISDFMTPFSKLVTGQHGISLDEANDIIWENKINTLPIIDKNQCLRYFVFRKDYDNH
                     KKNPNELLDSHKRLLVGAGVNTRDYMERIPKLVEAGVDVLCIDSSDGFSEWQKETIAF
                     VRSKYGDSVKIGAGNVVDRDGFNFLAQAGADFVKVGIGGGSICITREQKGIGRGQATA
                     LMDVAEARDELYRKEGIYVPICSDGGIVQDYHMVLALAMGADFIMMGRYFARFDESPT
                     KKMLIGGNYVKEYWGEGSNRARNWQRYDMGGNPALKFEEGVDGYVPYAGKLKDNLDIT
                     LGKVKSTMCSCGAFSIPELKKEAKITLVSSTSIIEGGVHDVILKDTKGKH"
     gene            8988..9377
                     /locus_tag="PKY68_02860"
     CDS             8988..9377
                     /locus_tag="PKY68_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24011.1"
                     /translation="MSYKVPVSYRFEAAVKKSLAFLCFFTAISFATEAQELDVAVDLQ
                     KDDTGVYNLTIPLPEGVYRVHLVLGSEESSGHTVVRAESRRLYATEIVTEKGEFKEFS
                     FTVHRRSPLISPQEKVRLKPREIGKLN"
BASE COUNT         2549 a         1969 c         2256 g         2600 t
ORIGIN      
        1 ccatatggaa aggttcggga tgtcccattt ctgcccgcag cgctccccag gtagtgcttg
       61 tatcgccatt ggcgaattca accagatcga tggcatcttg tatccagggg cccatatcct
      121 ccagaggcgc tacttctctg tttctgaact gacaggaaac accgacgggt aatacgggca
      181 ggggttttgc tccaaggtct tcgcaaaaaa ggaagtattc ataaaagccc aatccgtaag
      241 attggtgata gccccaaaga ttccagttgg gcttgcgtac cgccacatcg cctacggtat
      301 gtttccatcg gtaagcgttg tccagcccct tgccgtgaga gatgcagcct cccggaaaac
      361 ggacaaatct cggttgaaga tcggcgatag cctgagccag atcggcccga agtccgtttc
      421 ttctgttttt gaaggtgttc tcggggaaaa gcgaaaccat atccatatac acattcccgc
      481 tgccttgagg cagaatatac agtgcagcgt cattaaaagt aattttactt ttgagcgtta
      541 aatggtattg ctgccattca ctaccgatgg caggaatggt atcggaagca ataatataac
      601 ctccgctgct tttcaactgt actaccaccg ctttgtcgtg atcggtatcg cgtttgaggt
      661 aagccgaaaa gaaataattc tcgttttgtt taagggcaat accgtcaaag ccggtatttt
      721 taactcctgc ggccctaccc tgattgtata tgaccaattt cagacaattt gtgttgtgtt
      781 ggttcagagg atgatttttc accacactca gggctaccga ggctcctcct tcctgcacac
      841 ccgaccaggc cgttaagggg cctaggttgg tgtagccatc caccacataa tattcaaagg
      901 aacggttttg taccagttcg gcgtacaggc ctccgtcggc ggcgtagttg atgtcttcga
      961 aaaagacccc gatcaaatcc cgactcatgg gaacggtttc ctgatcaaga gtcagggaga
     1021 tgttaacctg agacaaagtt gaaggcaagt agcacaggac caaaaaggca atcgaaagga
     1081 ttcttgtttt catattgatt ttcttggaat gatggttgat tttgagcttc acaaatgcaa
     1141 atatagtgta tttatttaca aagccctagc gctttttgtt gcctttttgt tacattttat
     1201 ttgtgccggc ctttttagtc attttttatg aatttataat gaagtacaat tcaatcagat
     1261 atcttatttg gtttatattt tatgtttgtt taccggctgt atttgttctg gatttgaaaa
     1321 ttcttcccta tttttgaccg gtttttttat tcttctgctc gcgtgcagag gacaatgaaa
     1381 aagtatcaat atggggatat taaaagagaa gtttatcaat tatactgcac ctcaggaatt
     1441 gatggcggca ggagtttatc cttattttcg cgagattcag tcggatcagg atacagtggt
     1501 aatcatcaac ggaaagcgag tgttgatgtt tggatcaaac agttatctgg gtttgaccag
     1561 ccatcccaaa attaaagaag ccgccatccg ggctatcaaa aaatacggta ccggctgtgc
     1621 cggttctcgt tttttgaacg ggactctcga tattcatact caactggaag acagattggc
     1681 aaaattgacc ggcaaggagg ctgctctttg ctacagtacc ggttttcagg taaatttggg
     1741 agtggtttcc gtgttggagg gcgcaatgat tacctgatac tcgatgaact gaatcatgct
     1801 tccatcatag aaggctccag gttgtctttt tccaaaacca cgaagtatgc tcacaacgat
     1861 atggaagcct tggagcaaaa attaaaactt tgtgaaccgg gacacgatat tataattgtg
     1921 gtagacggca ttttttcgat ggaaggagat attgtccgtt taccggagat tctggctttg
     1981 gccaaaaaat acaaggcctc tgttatggtg gacgatgctc acgctctggg tgtgttggga
     2041 gaaaacggtg cgggcaccgc tgcacatttt ggccttaccg acgaggtgga actcattatg
     2101 ggtacctttt ccaaatcgct ggcttcgctc ggtggttaca tcgcggcaga caaggaagta
     2161 atcaatttta tcaagcataa ttcccgcccg cttattttca gtgcaagcat gacgccggct
     2221 tcggcagcca ctgctcttgc cgccctcgat attatggagc aggaacccga acgcattgag
     2281 catttgtgga agttgacgca ttatgccagg gaagctttca aggccgccgg tttcgatacc
     2341 ggtccttcgg aaacgcctat cattccgtta ttgatcaggg atgattttaa agccttgaaa
     2401 atgaccagaa tattgctcga ccggggtgtg tttgtcaatc cggttatttc gccggcagtg
     2461 cctaagggtt cgagcttgat tcgtttttca cttatggcaa cccatagttt tgaacaagtg
     2521 gacgaggccg tagataaagt gtcgggtgtt gccggggaac tgggtatctt tgatgcagta
     2581 tgaaaaaagt aattctcatt accggtgttt cttcaggctt tggaaaagag gctgctgttt
     2641 taatggccca ggccgggtat actgtttacg gaacggttag gaaaaccgtt aaaccgctgc
     2701 aaggcgtaaa tatactctat atggatttga cgcagccggc aagcattgat aaggctgttg
     2761 ccgaattgtt ggagcgcgaa aaacgtatcg atgtattgat caacaatgcc ggtatgcatt
     2821 taggaggccc tattgaggaa gctccttcgg aattgtatcg caggcaattg gacaccaatc
     2881 tgcacggtat ggttcattta ttgcaggccg tactgccaac gatgagagca caaaataaag
     2941 gcttaataat caatttcagc tctattgggg gcctgatggg tttgcctttc caggcttttt
     3001 attctgccag taaatttgcc atcgaaggat tgagcgaggc cttgagaatg gagctgcggg
     3061 ctttcggcat taaggttgtg cttgtcaatc cgggcgactt ccataccagt aataccgcca
     3121 accgtattaa cgtttgtgat caaaagggtc cttacgccag acagttcgag atttccaaag
     3181 cccgttttga atccgacgaa accgccggag gtcagcctgc cgtgatggcc cgtaaacttc
     3241 taaaaattat agcagccaaa caccctcgcc atcgctacgt tgtagcggcc ccggaccaaa
     3301 aattggccgt gttgctcaaa cgcatccttc cttcggattg gtttatgccg attttagcct
     3361 cttattatgg cattaagaag agggcaagca actgagtaaa gctgcccgat acgtcgccga
     3421 aaaccggcat taatccctaa aatccggtat gtattttgct taaattaccc tgttttagcg
     3481 attgtgagcc cattgtgtgt ccgttaaatt tgcgttcgtt tttaatagga aattgaactt
     3541 aaatgaaaaa gacgatttta tgcttaattg ctttattaac gatttctctg ctcggtgcgc
     3601 aggagaagac agatgccatg cttttcggtg atgtgaaaac cctcgatggg aaacaacatc
     3661 tctcctttat acagattcag gtcaaaggca ctaacattgg tacccttacc gatgcttccg
     3721 gacattttaa gatcgccaat ttacctctgg gaaaacaaat aatagtagcc aggggtctgg
     3781 ggtttattac tcaggagcgc gaagtattta tggaaagagg taaggctgtt actttatatt
     3841 ttgaactcga agaagacgcc atgaatctgg aacaggtggt aattaccggc accaggagcc
     3901 ggcattacat caaagacgtt cctgtaagaa cagaagttat aacggcaaaa accatagaga
     3961 ataaaaacgc agccaattta tatcgggccc tggaagggat tcccggtatc cgggtcgaaa
     4021 atcagtgcca ggcctgcaat tttacaatga tacgcatgca gggcttgggg gccgagcata
     4081 cccaggtatt gatcgatggc cagcctttgt attccggttt ggccggagtt tacggtttgc
     4141 agcaattcag tacggtggat gtggataaga ttgaagtcat caaaggtgcc ggttcggcct
     4201 tgtatggcag cggggcagtg gccggagcca tcaatatcat atcgagagag ccatcgtatc
     4261 aacctcaaac aaagatagaa acacagatag gcagccacgg tactcaacgt tacgacttgt
     4321 cctcgtctct gcgcaatgaa aaaggaaatg tgggattgac catttttgct caacgttatt
     4381 ccgaacaggc catcgatgaa accggagagg gcagcactcg cgaagaagtt cgcaaacccg
     4441 acggagtttc cgaccatgtt gattctgagc tgactaatct tggtttcagc ttgtttgtca
     4501 acgatgcctt tggaggcagt gatcaactgg tgctcagagg acgatcggtt tttgaacaac
     4561 gtcagggcgg ccttttgaca gacgattatt ataccaatcc ttttaccgac ggtaccgaaa
     4621 atatcgtcac caaacgttac gaggcttctt tgagttatca gaaaaagctg ggtctgcgtt
     4681 cggagatgaa tttcaatacg gcttacgtta atcatcaacg caatgcgacc aacgattcat
     4741 atctcnnnnn nnnnnattac ctggatactc acggtgattc agtgcccgat ttgagaatta
     4801 tgaggcctta tttagccgat gaacaaactt ttacatcgac tctttctttc agctcagccc
     4861 ttaatacgca tcgccttgtg tttggagctc aattctttta cgatcatatc gacgaatcgg
     4921 gcatgtatgt ggtggttgat gaatccagtt cttattacgg tgattcttac cgttccatgg
     4981 ccaccaaatc ggcccgtgaa gtcggcctgt tcgctcagga tgaatgggcc atcggtcgtt
     5041 ggatgctggt gccgggcctg cgtctggatc atcatcgttc aggtgagagc tataaggccg
     5101 atcgccaggt atcggatctt tctttatttc ccgaaacctc gttcgaagcc acgactctca
     5161 atccccgcct agctattcgc tataaattga gcgaccggat tacgctcaga gccaatgttg
     5221 gaaaaggttt tagagctcca tacggttttt ctgaagattt acatctttgc agcggttctc
     5281 ccagggtttg gaaatcttcg ggtctggaac ccgagacatc ggtcagttat aatttatcgg
     5341 cagattatta tggccgaaaa attcgtttga gtgccaattt attcaggact gacctgaaaa
     5401 acaaaatagg cttcagcgac gctgatgcac aagtggctgc tctgggttat gattatcagt
     5461 ggaaaaacat agacgacgcc tttgtacagg gcctggaact gacggcggtc tacagcccgg
     5521 tgagccaatt gcatttgagt atggatttca gcctgaaccg tggagagtac aaacaggagc
     5581 gtgaggattg gaaaggaact gcctacgaaa aggaaagccg ctatatttcg cgttttccat
     5641 ctactgccgg caactttatg ctcgaatacg ctcccgctaa ttgggcctta gtgttgaaca
     5701 gcaattatca gggatctatg tacattgatt attacaatac cgacatcgat cctttattgg
     5761 gtgatcaaag caagattaaa aaaacggagc gttttgttct gctcaacgcc aaagtatcac
     5821 gccgttttgg tcagtacaaa ctctatgccg gagttgacaa tattcttgat tacatacaag
     5881 acgaaaggca tttggacgac gcagcttatt tgtatgctcc aatttgcggc gttgtatttt
     5941 atgcaggatt gagcatagat atacgtcatt gattgtactt tgataaaata agatgtattt
     6001 ttgcttgaga tgtaattatg tatcagactc acaggacata tatcaagccg gatatgtgta
     6061 tggccgacct ggtcatcgag aatccttctc tcttgttgct cctggaacgc tttgggatgg
     6121 aaacggcatt gaacgatacc gatgttgctc agttgtgtga gcaatacgag gtaaatacgc
     6181 cggtattttt gttgctctgt aacttgtaca atggtttttt ccctcaacgt aaggatataa
     6241 atccggagca acaccgattg accgacataa ttaaatacat caaaaacagt catcgttttt
     6301 atacattcga aaaatatccg caaatccggg attatattgc acagctctat gaacgtcagt
     6361 gtccggaaga tattcagcaa ttggaaattt tcttcgggga ctatttcgag gaagtacaag
     6421 agcatttacg ttacgaagaa gaggtggtat ttccttattg cagttattta ttagagggca
     6481 aagcgaaggg ggaaatcaat gctttctctt cggcagaata ccgggatcat cataccgata
     6541 ttgaaacaaa acttgcggac ttaaaaaatt taatgctgaa acatttgtcg cttgacggag
     6601 attttattct gaagcgcaaa atactttatg cattgatgga gctcgaaaac gacctataca
     6661 tacattctca ggtagaggaa ttggtgctgc tccctttgtt ggagcatctg gaattcaaag
     6721 acaggaatgg ataaatgtcg tgtcaatatt gctattctgg aaccttccac tgttttgtac
     6781 gaaggcttgt cgacgatttt gctaaaaact catcatcatt attatttttt tcgttttgca
     6841 gacctggagg aactggcggt catgtacaag ctgactcctt ttgatatcgt ggtagctaat
     6901 ccttgtatgc tgcaaaataa acagaaagcc tttgtccgtt taaagaaaaa aatgccccaa
     6961 gtgtattgga tagctttggt gtactctttt tacgacgacg aattgctgag tcttttcgat
     7021 aacaaattca gtataacgga agatcccaaa gtaatgctga attgttttga aagggcgtgt
     7081 gacagttgta aatgcaatga aaaaccgggc aaaaaaaagc tgttgagcga acgtgaaaca
     7141 gaggtgctga tagccttgac caaaggcttg gcaaacaaag aaatagccga aatgatgaat
     7201 attagtatac acaccgtaat gacgcatcgc aaaaacatcg tcgaaaaaac aggtttacac
     7261 agcttgccgg ccctgactat gtacgccata tcaaaaaaac tgattgcttt tgagcccgga
     7321 ggtgagcctt aaatacacaa aactttaatt cttttgaatc atgatggttt ttgagcaaaa
     7381 agccgtaaat ttgcccaatt ttaatcttga gtattatggc attgcttaaa gaagaaatct
     7441 cccgaacatt caacgaatat ttgctgatac ccggactaac gaccaaagac tgtgttcccg
     7501 aaaaaattga tttgagaact ccgttagttc gttattctaa agggcaaagc tctgaaatac
     7561 aattgaatat accttttgtg tcggctatca tgcaagctgt ttcggattcc aatatggcga
     7621 ttgccctggc caaaaacgga ggtttatcct ttattttcgg ctcgcaaccc attgaagaac
     7681 aggccgagat ggtgcgcaaa gtaaaaaaac acaaagccgg ttttgtggtc agtgatgcca
     7741 acctaaggcc tgatcagcgt ttgcaggatg taatcgattt gaaggccaaa acaggttttt
     7801 cgacgatagg cattacccac gatggcagtt ccaacggaaa attgctgggc attgttacca
     7861 gcagggatta tcgcccgggt aaggacgatc cgtcaaaaat aatcagcgac tttatgactc
     7921 ctttttccaa gttagtcacc ggccaacacg gtatcagcct ggacgaagcc aacgacatca
     7981 tctgggaaaa caaaatcaat acgctgccca ttatcgacaa gaaccaatgt ctgaggtatt
     8041 ttgtcttccg caaggattac gacaatcaca aaaagaatcc caacgaactt ttggacagtc
     8101 ataaacgttt actggtaggg gcaggagtca acacccggga ttacatggaa cgcataccca
     8161 aattggtgga ggcaggggtg gatgtgttat gcatagattc gtcggacggc ttttcggaat
     8221 ggcaaaaaga aaccattgct tttgtacgca gtaaatacgg cgacagcgtc aagatcggag
     8281 ccggcaacgt agtagaccgg gatggattca attttttggc tcaggccgga gccgattttg
     8341 tcaaagtggg tataggagga ggctccatct gtattacccg tgaacaaaaa ggcatcggcc
     8401 ggggacaggc tactgctttg atggatgtgg ctgaagccag ggacgaactc taccgaaagg
     8461 aaggtattta tgttcccatt tgttccgacg gcggtatagt tcaggattac cacatggttt
     8521 tggctttggc catgggagcc gatttcatta tgatgggtcg ctatttcgcc cgtttcgacg
     8581 aaagccccac caaaaaaatg cttatagggg gcaattacgt aaaggaatat tggggcgaag
     8641 gctccaatcg tgcccgcaac tggcagcgtt acgatatggg cggcaatcct gcactcaaat
     8701 ttgaagaagg agtggacggt tatgtgcctt atgccggcaa gctcaaggat aatttggata
     8761 ttaccctggg caaagtcaaa tcgaccatgt gcagttgcgg tgctttttcc atacctgaac
     8821 taaaaaagga ggccaagatt acgctggtct catctaccag tatcatagaa gggggtgtac
     8881 acgacgtgat cctcaaagac accaagggca agcattaatg cttgtttcag cctaattctg
     8941 atatctgctg tttactcagc tcttgtattc atttttttag ggaagacgtg agttataaag
     9001 ttcctgtaag ttataggttt gaggctgccg ttaaaaagag ccttgctttt ctgtgttttt
     9061 tcacggcaat tagttttgca acggaggccc aagagctgga cgtggccgtt gatttgcaga
     9121 aagacgatac gggtgtgtat aatctgacta tccctttgcc cgaaggagtg tatcgtgttc
     9181 atttggtatt gggttcagaa gaatcgtccg gtcatacggt tgtccgggcg gaatcgcgtc
     9241 gtttgtatgc cacggagatt gttacggaaa agggagagtt caaagagttc tcgtttaccg
     9301 tacacaggcg gagcccgctt atttcgcctc aggaaaaggt caggttaaag cccagggaaa
     9361 tcggcaaact caattgagaa aatc
//
LOCUS       DAOSGL010000053         9342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6277_length_9342_cov_4.349736, whole genome shotgun sequence.
ACCESSION   DAOSGL010000053 DAOSGL010000000
VERSION     DAOSGL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6277_length_9342_cov_4.349736"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /gene="gpmI"
                     /locus_tag="PKY68_02865"
     CDS             <1..1290
                     /gene="gpmI"
                     /locus_tag="PKY68_02865"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOH24012.1"
                     /translation="KINISCRDISILENPGIKAAYGYAKQSGKQIHFLGLVSDGGVHS
                     SLDHLFKLIEISKLYDIKKSFVHCFMDGRDTDPKSGKSFIAALQKHLDRNTGAIASIV
                     GRYYAMDRDKRWERIKEAYDLLVKGKGVKAQDMVQAVQASYDQGVTDEFIKPIVHTDA
                     QGQPLATIQDGDVVLFFNFRNDRARELTIVLTQEDMPQHGMQTLCLEYYTMTPYDATF
                     KGINVLFDKENVDNTLGEIVAKAGKKQLRIAETEKYAHVTFFFSGGREKEFENEDRIL
                     IPSPKVATYDLKPEMSVNEVGDALKQAIETEKYDLIVVNYANGDMVGHTGVYSAIQAA
                     VVAVDANIKKVVEAAKSKDYEVIIIADHGNADNALNPDGSPNTAHSLNPVPFVYISRN
                     SSAKVSDGILADVAPSLCHILGIKQPAEMTGRCLIHD"
     gene            1300..1887
                     /locus_tag="PKY68_02870"
     CDS             1300..1887
                     /locus_tag="PKY68_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOH24013.1"
                     /translation="MLQIDKTIVSLDIINQTFCCDLNKCKGRCCVEGVSGAPLEEDEI
                     AELEKALPAVWDELSPRARQLINEQGVCYKDASGETVTSIVDGKDCVFTYYDEKGVCY
                     CALEKAWREGKSSFIKPISCHLYPIRLSKLTSGVVALNYQQWHVCVPALAKGKTLGLP
                     IYRFLREPLIRKFGQDWYDQLCEAAKMLEDYPRNV"
     gene            complement(1993..2346)
                     /locus_tag="PKY68_02875"
                     /pseudo
     CDS             complement(1993..2346)
                     /locus_tag="PKY68_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
     gene            2447..3373
                     /locus_tag="PKY68_02880"
     CDS             2447..3373
                     /locus_tag="PKY68_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOH24014.1"
                     /translation="MKKQPIILCFFLWTALAAFAQERIDLPLWPDGVPESNGITAEEI
                     NENNYFSNVSVPAMSVFKANPDKNTGAALIICPGGGYARQAAGHEGFQLTGWLNENGI
                     TAIILKYRLPNGHDFIPSKDALQAIRIVRSKAEEWGINPSKIGISGFSAGGHLASTAG
                     THFDYGNPEAEDPIARQSSRPDYMILFYPVISMKEGVTHVGSKRNLLGDNPTKEQMLE
                     YSNELKVSAETPPTLLLLSDDDKGVIPQNSLDFYAALKNHKVPAAMYIFPEGGHGWGY
                     KENFRYHHVWRILMIEWMMQQKILPDMIRQQN"
     gene            complement(3379..5802)
                     /gene="pbpC"
                     /locus_tag="PKY68_02885"
     CDS             complement(3379..5802)
                     /gene="pbpC"
                     /locus_tag="PKY68_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOH24015.1"
                     /translation="MFLLLVVLFLLIPLPKFKYPCSTVVYAANQELLGARIAADGQWR
                     FSPEGEIPDNYVKALILYEDKYFYYHPGVNPVSLGRALVQNLKAGRIVSGGSTLSMQV
                     ARMSRNNPPRTLSGKCLEILMALKLELCYSKDKILALYAGVAPFGGNVVGITAASWRY
                     FNRPPQKLSWAEAATLAVLPNAPALIHPGRNAAELKQKRDALLGQLYQKKHIDSLSYQ
                     LALAESLPQAPIPLPGLALHVVEKAHKQFPGQNVYTSIDYPLQEKCIALLEQRSKELE
                     QSEIHNLAALVCDVKQNSILAYAGNISAVAQLDVFVDIVQTPRSSGSILKPFLYASML
                     HQGEILPNTLIADVPVNFSGFSPKNFDLNYQGAVPASEALSQSLNVPAVEMLHQFGEA
                     RFLDVLKNLGFTTFVYPSQHYGLSLILGGGECNLLELAGAYASLARVLNRFGSVQEYS
                     SEDYRPLRLFRENNPDKKNGTKAFQEANISRFWPEIFPNKRKKAVSKGRKSASENEES
                     LPLLSASSIWCTMEALRQVNRPRERSGWKHFESSRRIAWKTGTSFGLRDAWALGTTPE
                     HVVAVWAGNADGEGRPGLTGLRAAAPALFDLFDLLPQSGAWFEQPDSEMNLEEICMES
                     GCKASVYCTRKALQMVPASGLKTGLCPYHRLVHVSKDGKFRVNASCYPLELIRSDSIF
                     VLPPAMEWFYRKTHPSYRGLPPLMESCGEEKEYKQMEILYPRNPYSLYIPRELDGSPG
                     KLVIEVVHRKADVLLFWHLDDEWIGNTRRIHQMSISPAPGNHTLSIYDQQGNNLIQHL
                     RIQAGRVER"
     gene            complement(5881..>9342)
                     /locus_tag="PKY68_02890"
     CDS             complement(5881..>9342)
                     /locus_tag="PKY68_02890"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOH24016.1"
                     /translation="TSKDDPTGNWKVVVKAGTHIFQRGIQVESIKPNRLKIELEISED
                     VLSKSETSWAKLKASWLHGTPAANMKAQVSAVLHPHTPTFEKYKDFDFSTPYPRQSSK
                     EFTLFDGQLDYKGEASVTMSFGPGRDFEGYMQARISTKVYEGGGFSINQRSIPFIASE
                     HFVGLKMNYSYPSWKKLDNDKNHRVEVVSVDEKGNPADLSNIEVKLYDLDYHWWYQGN
                     SNLLASYAGQTYHKPVYSTKISTSKGKGSFELSNNSQRYGCYLLLVIAPDGHTCGQQI
                     YFGLPWGSGIQKGDAQVLALVAERDQYKTGETASLSFPASKDARALVTLENGRGILHQ
                     EWLDNLEAMNEYRFVTTPDMAPNVYVNIMLIQAYAQTANDLPIRLFGLTSFRVEDEQT
                     RLHPVLDIPDELRPEKEYTIRVSESNKQAMDYTIALVDEGLLDLTNFSTPDPWAHFYA
                     KEALGTKTYDMYNEVLGSFGSRLESMYAIGGSDQEIDYSKKKADRFVPIVEVLGPFKL
                     PAGKKAEHKIQLKPYAGSVRAMLIAAGRGRYGSMEKSIRVSEPLMLYASLPRTLALGE
                     KADLPLTVFAMDESIKKVEIRFETEGRLQGSADTLILFDAPGQQDVFFDLKAAGEAGL
                     GIIKLSAVSGREKTQQSIELDVMHNNPPSTEQVFKMLPAGESWQANLSALGVPGTNKA
                     RLEISSVLPLNLEYQLSFLNQYPHQCAEQVVSAVFPLIYVPKLLDLSAEKRKEYEQNI
                     KDCIDRLPRYQSMDRGFAFWPGRSAGELWLTSYLTYFMLEAEKAAYLIPSNLKRAGLD
                     YLANGVRRYREYLPDYKAQIQAYALFVLARAGEPEVAAMNRLMKADCLNNQGKWLLAS
                     AYAASGMKEAAYKLIDLRDTEPGTVSVYTYGSVLRDRCYLLQSLMLLGEWEQAAPLAL
                     KIATDFNRTGRHSTQTTGFVLSVLSEFCLLTSATSEAETELYVNGKPLLTQSSGAFTL
                     VDLSHGRDLELDLRLVNKGKGTVFASLFNQGVKAGMDASEQEKGLALEISYMDQEGKK
                     LNVSKLKQGTNFIVQLRISNKTAFAVDNLALTQMIPAGWEIINERLFGTGSASEDQNN
                     YDYRDIRDDRVHTYFSLGAGKTKVFTLELNASYCGDFILAPVLCEAMYDQAYYARKGG
                     AKVKVER"
BASE COUNT         2434 a         2346 c         2182 g         2380 t
ORIGIN      
        1 aaaatcaata tttcctgccg cgacatcagc attctcgaaa atcccggcat caaggcagcc
       61 tacggttacg caaaacaatc gggcaagcaa atccactttc tgggcctggt ttcagacggc
      121 ggcgtacaca gctcgctcga tcatttgttt aagcttattg aaatctccaa gctttacgac
      181 ataaagaaat cctttgtgca ttgctttatg gacggccggg acaccgaccc caaaagcggt
      241 aagtcgttca tagccgctct gcaaaagcac ctggatcgaa acacaggggc catagcctct
      301 attgtgggcc gttactatgc catggacagg gacaaacgct gggagcgcat caaagaagcc
      361 tacgatttgc tggtaaaggg caaaggagtg aaagcacaag acatggttca ggctgttcag
      421 gcatcgtacg atcagggagt aaccgatgaa ttcataaaac ccattgttca tacggatgct
      481 caaggccaac ctctggccac cattcaggac ggcgacgtgg tgctcttttt caatttccgc
      541 aacgatagag ccagagagct cactatagta ctgacccagg aagacatgcc acaacacggt
      601 atgcagacgc tctgcctgga atactacacc atgacccctt acgatgccac cttcaagggc
      661 ataaacgtac ttttcgataa ggaaaacgtc gacaataccc tgggtgaaat tgtggcaaaa
      721 gccggcaaaa aacaactgcg tatagcagaa accgaaaagt acgctcatgt aaccttcttt
      781 ttctcgggcg gacgcgaaaa agaattcgaa aacgaagacc gcatactgat tccatcgccc
      841 aaagtggcca cctacgacct gaaacccgaa atgagcgtta acgaagtggg tgacgcgctg
      901 aagcaagcca tagaaaccga aaaatacgat ctgattgtcg taaactatgc caatggcgat
      961 atggtcggcc acacgggagt ctattcagcc attcaggccg ccgtcgtagc tgtggacgcc
     1021 aatattaaaa aggttgttga agcagccaaa agcaaagact acgaagtaat cataattgcc
     1081 gatcacggca acgccgacaa tgccttgaat cccgacggtt ctcccaacac ggcccattct
     1141 ctgaatccgg ttccttttgt gtacataagc cggaatagtt cagccaaagt cagcgatggc
     1201 atactggccg atgtggcgcc ctcgctctgc cacatcctgg gcataaaaca gcctgcggaa
     1261 atgaccggac gctgtcttat acacgactaa ttacggagca tgctacaaat tgataaaacc
     1321 atcgtcagcc tcgatataat caatcaaacg ttttgctgcg acctgaacaa atgcaaaggc
     1381 aggtgttgtg tggaaggggt gtcgggtgct ccgctggaag aagacgaaat tgccgaactg
     1441 gaaaaggctc tacccgcagt atgggacgaa ctttcgcccc gcgcccgtca actcattaac
     1501 gaacagggag tttgttacaa ggatgcttcg ggcgaaaccg ttacttccat cgtagacggt
     1561 aaagattgtg tctttactta ttacgatgaa aagggcgttt gttattgtgc tcttgaaaag
     1621 gcctggagag aaggtaaaag ctcatttatc aaacccatct cctgccacct ctaccccata
     1681 agactttcta aactgacttc cggagtcgtt gctctcaatt accaacaatg gcatgtctgt
     1741 gttcccgccc tggccaaagg aaaaacttta ggtttgccga tttatcgctt tctcagggag
     1801 cccttaatcc gtaaattcgg acaggactgg tacgatcagc tctgcgaagc cgccaaaatg
     1861 cttgaggatt atccccggaa tgtttaatta ccgactcctc ccccttttac ggttattgtt
     1921 cggatttgtt ttttctctga ttttatccca ggcataagtg ccggcttttt gccgtttgga
     1981 actgtttata tccaacgttt taaacgataa aatatgcacg ctgaccccca gagcgatcaa
     2041 gagtaataaa attttaagcc aacaaatttc cagaaaaaaa agaactgcaa tcaggatgct
     2101 tatccacaac aaaacaaccg agaatatttt ggtgcccaaa ggaatcgcct tatgctttct
     2161 gtaattttct atatagcacc ctaaacaggg atgttgcatc agccagcggt aataacgatc
     2221 ggaaccctta tagaaaagag aagcgctcag caacaaaaaa ggcgtggttg gtaaaagcgg
     2281 caaaaaaaca cccagtatcg ccaaggccaa aaacaaaact cccagcacgt tatagacaac
     2341 tttcattctt ttccggaatt ggagcacaaa agtagttttt tacaccgagt ctttaaaatt
     2401 ctggcagaat cctttatatt tgctctgatc ttaactcatt taaatcatga aaaaacaacc
     2461 gatcatacta tgtttttttc tgtggacagc tttggctgcc ttcgcacaag agagaataga
     2521 cctgccttta tggccggatg gagttcccga aagtaacggc attactgccg aagaaatcaa
     2581 cgaaaacaat tatttttcca acgtcagtgt tccagccatg tcggttttca aagctaatcc
     2641 cgacaaaaat acgggtgcgg ccctgattat ttgtccggga ggaggctacg cccggcaggc
     2701 tgccggacac gaaggctttc agctgaccgg ctggctcaac gaaaacggca ttaccgccat
     2761 aatcctgaaa taccgtttgc ccaacggaca cgatttcata ccttcgaaag acgccttgca
     2821 ggccatcaga atagtgcgaa gcaaggccga agaatgggga atcaacccat ccaaaatagg
     2881 tatttccggc ttttcggcgg gcggccatct ggcttcgacc gcaggtactc acttcgatta
     2941 cggcaatccc gaagccgaag atcccatcgc ccgtcaaagc agcaggccgg attatatgat
     3001 cctcttctat ccggttattt ccatgaaaga gggtgtcaca cacgtcggtt ccaaacgcaa
     3061 cctattgggt gacaacccaa cgaaagaaca aatgttggag tattccaacg aacttaaggt
     3121 aagcgctgaa acaccgccaa ccttgctgct gctcagcgac gacgacaaag gagtaatacc
     3181 gcaaaacagc cttgattttt atgcagcgct aaaaaaccat aaggtccccg ctgctatgta
     3241 tattttcccc gaagggggcc acggctgggg ctacaaggag aacttccgct atcaccatgt
     3301 ctggcgcatc ctgatgatag aatggatgat gcagcaaaag attctgcccg acatgatcag
     3361 gcaacaaaat taaagagctt acctctcgac tctgccggcc tgtattctta aatgttgaat
     3421 cagattgttg ccctgctgat cgtaaatact aagtgtatga ttcccgggcg cagggctgat
     3481 gctcatttgg tgtatcctgc gggtattgcc tatccactcg tcgtcgagat gccaaaacaa
     3541 aaggacatct gctttgcggt gtaccacttc gattaccaac ttgcccggac ttccgtcaag
     3601 ctctctgggg atatacaaac tgtatggatt acggggatac aatatttcca tctgcttgta
     3661 ttctttttct tcaccacagc tttccataag aggaggcagg ccccggtaag acgggtgagt
     3721 tttgcggtaa aaccactcca tagccggcgg taacacaaag attgaatcac tgcggataag
     3781 ttcaagcgga tagcaggagg cattcaccct gaattttccg tctttggata catgtaccag
     3841 ccggtgataa ggacaaagtc ccgtctttaa gcctgatgca ggaaccattt gcaaggcttt
     3901 tctagtacaa taaaccgatg ctttacagcc actttccata catatttctt ccaggttcat
     3961 ctcgctgtcg ggctgttcaa accaagctcc gctctgcggt aataaatcaa aaagatcaaa
     4021 cagggcggga gcggccgctc tcaagcctgt aagcccggga cgcccctcac cgtcggcatt
     4081 gcccgcccat acggctacta cgtgttcggg agtagttcca agggcccatg cgtcgcgtaa
     4141 accaaaactg gttccggttt tccaggcaat ccgccggctg ctttcaaaat gcttccagcc
     4201 gctacgttct cggggacgat ttacttgtcg caaagcttcc atggtgcacc atatggagga
     4261 tgccgacagt aaaggcaaag attcctcgtt ttcggaggcg gactttcggc ctttactaac
     4321 agctttcttc cttttattag ggaatatttc cggccaaaag cgactgatat ttgcctcttg
     4381 aaatgccttt gtaccgttct ttttgtcggg attattttct ctgaaaagtc gtaaaggacg
     4441 gtaatcttcc gacgaatatt cctgaactga tccaaagcgg tttagtaccc ttgccaaaga
     4501 agcataggcc ccggccaatt cgagcagatt gcattctcct ccgcccagaa tcagcgacaa
     4561 accataatgc tgggacggat aaacaaaagt ggtgaaaccc aggtttttca gaacatcgag
     4621 aaaacgagct tctccaaact gatgtaacat ttccacagcc ggcacattga gggattggct
     4681 caaggcctca gaagccggaa ctgccccctg gtagttaaga tcaaaatttt tggggctgaa
     4741 acccgaaaaa ttcaccggaa catcggcaat aagagtatta gggagaattt ctccctgatg
     4801 cagcatcgag gcgtacaaaa atggcttaag aatgcttccg ctgctgcgtg gggtctgcac
     4861 gatatccaca aaaacatcga gttgagccac cgcgctgatg ttgcccgcat aggccagaat
     4921 actgttttgc tttacatcgc agaccagggc agccaaatta tggatttcgc tttgctccag
     4981 ttctttgctc cgctgttcca gcaaagcgat gcatttttcc tgtagcggat aatctatgct
     5041 tgtatagaca ttttggcccg gaaactgttt atgggctttt tccactacat gcagggccaa
     5101 accgggcaag ggtataggtg cctgcggcag tgattcggcc aaagccaact gataactaag
     5161 actgtcaatg tgtttttttt gataaagctg cccgagcaaa gcatcccttt tttgtttcaa
     5221 ttcggctgcg tttctgccgg gatggatcag agcgggagca ttaggcaaaa cggccaaggt
     5281 tgctgcttcg gcccaggaca acttttgagg cggacggtta aaataacgcc aggaagcagc
     5341 tgtgataccc accacattgc cgccgaaagg agccaccccg gcgtaaagag ccaatatttt
     5401 atctttggaa taacacaatt cgagtttcaa agccatcaga atctctaggc atttcccgct
     5461 cagggttctg gggggattat tgcgagacat tctggccacc tgcatactca gggtacttcc
     5521 gccacttacg atgcgtccgg ctttgagatt ttgaacaagc gccctgccca gcgaaaccgg
     5581 attgacgccc ggatgataat aaaaatattt atcctcgtaa agaatcagag ccttgacgta
     5641 attgtcgggg atttctccct cggggctaaa gcgccactgc ccgtcagcgg ctattcttgc
     5701 acccaacagc tcctgattgg cggcatagac cacagtagag cagggatact tgaatttggg
     5761 aagaggaatc agcaaaaaca atacaacaag cagcaggaac aagccggtaa gtattttcag
     5821 ccgctttgtt ccttttgagc tttggttttt gaaacacatt ccttatgagt ttgcccggtt
     5881 ttagcgttct acttttacct tagccccgcc ttttctggca taataagctt ggtcgtacat
     5941 ggcttcgcac agcaccggag ccagaataaa gtcaccacaa taggaggcat tcagttccag
     6001 ggtgaacacc tttgttttgc cggctcccag cgaaaaataa gtatggaccc tgtcgtcgcg
     6061 aatatccctg taatcgtagt tattttgatc ctcacttgcc gacccggtac caaaaagcct
     6121 ctcgttgatt atttcccagc cggcgggtat catttgggtc aaagccaggt tatcgacagc
     6181 aaaagccgtc ttgttgctga tgcgcaactg aacgatgaaa ttagttccct gttttagttt
     6241 gctgacattc agctttttac cttcttggtc catatagctg atttcaaggg ccaatccctt
     6301 ttcctgttcc gaggcatcca tccctgcttt gacgccctgg ttgaaaaggc tggcaaagac
     6361 agtgccttta cccttgttca ccaagcgcaa gtccagttca agatcccggc catgggatag
     6421 atccaccaag gtaaaggctc cggaagattg ggttagtagc ggcttcccgt ttacatacaa
     6481 ttctgtctcc gcttcggaag tcgctgaggt taacaggcaa aattccgaca acacggaaag
     6541 aacaaaaccg gtggtttgcg tactgtgccg gcctgttctg ttgaaatccg tggctatttt
     6601 aagagccagc ggggcggcct gttcccactc tcccaacagc atcaggcttt gcaacaaata
     6661 acagcggtca cgcaaaacgg agccgtaagt ataaacgctg acggtgcccg gttcggtgtc
     6721 gcgcaggtct atcagtttat aagcggcctc tttcatccca gaggcagcat aagcagaagc
     6781 cagcagccat ttcccctggt tgttcaggca atcggccttc atcagccggt tcatagctgc
     6841 cacctcgggt tcaccggccc tggcaagaac aaacaatgca taggcttgaa tctgggcctt
     6901 gtagtcgggt aaatattccc ggtagcggcg taccccgttt gccaggtagt caagaccggc
     6961 tctctttaaa ttggagggta ttaaataagc tgctttttcg gcctcgagca taaaataagt
     7021 caggtaggaa gtcagccaca gctcgcccgc cgaccgtccg ggccagaagg caaaacctct
     7081 gtccatgctt tgataacggg gaagtcgatc gatacaatct tttatgttct gttcatattc
     7141 cttgcgtttt tcagcgctca aatccagcag tttcggcaca taaatcaagg ggaaaacagc
     7201 cgatacaacc tgctcggcac attggtgagg gtattggttg aggaaactca actgatactc
     7261 taagtttagt ggcaataccg acgaaatttc gagtctggcc ttattggttc cgggtacacc
     7321 caaagcagac aaattcgctt gccagctttc gcctgcgggc agcattttaa agacttgttc
     7381 cgtactggga ggattgttgt gcataacatc cagctctatg ctctgttgcg ttttttctct
     7441 gccggatact gcgctaagct ttatgatgcc caaaccggct tccccggctg ctttaagatc
     7501 gaaaaagaca tcctgttggc cgggagcatc aaaaagaatc agggtatctg cagaaccctg
     7561 gagtctgcct tccgtttcaa aacgtatctc gacttttttt atggattcat ccatggcaaa
     7621 gaccgtaagg ggcaaatcgg ctttctcgcc taaggccagg gttctgggta aactggcata
     7681 caacatcaaa ggttcgctga cccggatgct tttttccatg ctcccgtaac gaccccggcc
     7741 ggcagcaatc agcatggccc ggacagagcc tgcgtagggt ttgagctgta ttttatgctc
     7801 ggcttttttc ccagccggta gtttgaaagg gcccaagact tctacgatgg ggacaaaacg
     7861 gtcggctttt ttcttggagt aatctatttc ctgatcggag cctcctatgg cgtacatgct
     7921 ttccagccgg ctgccgaatg aacccaacac ctcattgtac atatcataag ttttggttcc
     7981 cagggcttct ttggcgtaga aatgagccca gggatcggga gtgctgaaat ttgtcagatc
     8041 cagcaaacct tcgtccacca acgctatggt ataatccatg gcttgtttgt tgctttcgct
     8101 tacccggatg gtatattctt tttcggggcg gagctcatcg ggaatgtcca gtaccggatg
     8161 caatcgagtc tgctcatctt cgactctgaa cgaagttagc ccaaaaagcc ttataggcag
     8221 atcgttggct gtttgcgcat aggcctgtat cagcataatg tttacataaa cattgggcgc
     8281 catgtcgggc gtagtaacaa aacggtattc gttcatagcc tccagattgt ccagccattc
     8341 ctgatgcagg atgcctctgc cgttttccag cgtaaccagc gcccgggcat ccttgcttgc
     8401 cggaaaactg agactggctg tttctccggt cttatattga tcgcgctcag caaccaaagc
     8461 caacacttga gcgtcgcctt tttgtatgcc gctaccccag ggcagaccga aatagatttg
     8521 ctggccgcag gtatgcccgt ccggggctat aaccaacaat aaataacaac catagcgctg
     8581 cgaattgttg ctcagttcga aactgccttt tcccttactg gtgctgattt ttgtgctgta
     8641 aaccggttta tgataagttt ggccggcgta ggaagccaac aggttactgt tgccttgata
     8701 ccaccaatgg tagtccaggt cgtatagctt aacttctata ttgctcaggt ccgccgggtt
     8761 gcccttttcg tcaacactca ctacttcgac ccggtggttt ttatcattgt ccagtttttt
     8821 ccacgatgga taggaataat tcattttaag gccgacaaaa tgttcgctgg caataaaagg
     8881 aatactgcgt tgattgatac tgaaaccgcc gccctcgtac actttggtgc tgattctggc
     8941 ctgcatataa ccttcaaagt ccctaccagg accaaaagac atagttacgg aagcttctcc
     9001 tttgtagtcg agctggccgt cgaacaaagt gaattctttg ctggattgac gaggataagg
     9061 ggtagaaaaa tcgaaatcct tgtatttttc aaatgtgggt gtatggggat gcaatacggc
     9121 gctcacctga gctttcatat tggctgccgg cgtaccgtgc aaccaggagg ctttgagttt
     9181 tgcccagctt gtttcggatt tactcaatac atcttcgctg atttccagtt caattttcag
     9241 gcggttaggt tttatgcttt ccacttgtat gccgcgctga aaaatatggg taccggcttt
     9301 aaccactact ttccagttgc cggtaggatc gtctttgctt gt
//
LOCUS       DAOSGL010000054         9129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6463_length_9129_cov_4.957571, whole genome shotgun sequence.
ACCESSION   DAOSGL010000054 DAOSGL010000000
VERSION     DAOSGL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6463_length_9129_cov_4.957571"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2266
                     /gene="secD"
                     /locus_tag="PKY68_02895"
     CDS             <1..2266
                     /gene="secD"
                     /locus_tag="PKY68_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOH24017.1"
                     /translation="EEVVVDFPIAVPQKEQVDEAAPVEKAPAEQVSDTAKTEEERLLE
                     QLAAETEIQTDEQSLEEWKREYPLFAVLQINQSQQGIGAGPCVGYALGRDTAKVNEYL
                     NSRNVRELLPRDVKFLWSVKPINEKTPLFELIAIKVTNRDGRAALEGDVITDARDDFE
                     QMSGSAVVSMSMNAEGSKTWARITKDNIGKCVAIVLDNYVYSYPRVNTEITGGSSQIT
                     GNFTSAEAKDLANVLKSGKMPAPARIVQEDIVGPSLGKEAIKSGLISFAIAFAIVLIY
                     MIFFYGIIPGLVADFALLCNIFFLMGILASFQAVLTLPGIAGIVLTLGMAIDANVLIN
                     ERIKEELAAGKSIKKALPDGYKNAFSAIFDSNLTTLLTGIILFILGTGPIKGFATTLS
                     IGILTSFFSGVFITRIIFERLLAKEKLNNITFTTKLSKNILRNPQVNFLGLRKKGYIV
                     AGVLVLACILSWSTRGLSQGIDFSGGRNYVVRFDQPVSTQDVRNSLDGIFEDASLSVI
                     TIGSENQVRVSTNYKINETYDELDAEIDSILFTGLKDYLPENTGLESFVEDYIVSSQK
                     VGPTVASDIKISAIWSVVAALLVIGLYILLRFRNVAFSVGVVASLAFVTIFIIGLYSM
                     LYSVMPFSLEIDQTFIAAILTVIGYAVNDSVVIFDRIRETTGLYPKRHKRLTMNSAMN
                     TTLLRTFSTAFSTALVLLAILLFGGDTIRGFAFAMLIGVVFGTLGTLFIASPVAYDIQ
                     RKREKFSDEVTEEE"
     gene            complement(2218..2898)
                     /locus_tag="PKY68_02900"
     CDS             complement(2218..2898)
                     /locus_tag="PKY68_02900"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HOH24018.1"
                     /translation="MRKVVLLLLITCFTVPGVWSRSVVECFKDMPADLLPGLNTAMKL
                     DMVDFFNSGKTARMLGLLGSEMELKILTEDYLMLQTSAASNVQLKLLPLKNGSRILAV
                     VNTVKAPAASSDLEFYNLDWQPIDTLAKPRLSAASFLNQDCLQQKGHAQDMSVEELNS
                     LYFYEYNFQSGNNFLLVYSSLPDFLGEEAFAGYRDCFNQRLVLEWTGKGFLYSSSVTS
                     SLNFSLLR"
     gene            complement(2912..3814)
                     /locus_tag="PKY68_02905"
     CDS             complement(2912..3814)
                     /locus_tag="PKY68_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOH24019.1"
                     /translation="MWLWATFASSLLLGVYEVYKKLSLKENAVVPVLFLTTVFCSLFF
                     LPLLLLSEWAPQSLENTLFFVPRADAKTHLFILLKSFIVLSSWAAAYYAIKNLPLTIV
                     SPIKSTQPVFVLMGAFMVFGERLNLWQWLGVLVSIISIFLYSYVGKKEGISFRRNPWI
                     WCLFLSVLIGSVSGLYDKYMMVNFDRMTVQVWYTIYQIFIMLPLLLIYMHRRKRGKEQ
                     AFDFRWSIVGLAVFLCLADFLYFYALSRPGALISVISPIRRSGVLIPFVVGALFFKDK
                     NIKAKAWILLGVFIGMCCLYLGSK"
     gene            complement(3825..4580)
                     /gene="truA"
                     /locus_tag="PKY68_02910"
     CDS             complement(3825..4580)
                     /gene="truA"
                     /locus_tag="PKY68_02910"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOH24020.1"
                     /translation="MQRFFLNFSYDGSAYHGWQIQPNAISVQEVMTSVLRTLFEKDLD
                     IIAAGRTDAGVHALAMTAHFDTAHPIENPDATAHKLDRMLPADIAVRSLRRVRPGAHA
                     RFDAISRTYQYKITLQKDPFLRDYSLRLYYEPDVEAMNKAAGRLLEYSDFTSFSKVHT
                     DVKTFICKVTHARWERRGSELIFTIQADRFLRNMVRAIVGSLLEVGKGRIDEEGFCRI
                     IEARDRCRAGTSVPAQALYLTEVKYSDDLYLIG"
     gene            4676..5662
                     /locus_tag="PKY68_02915"
     CDS             4676..5662
                     /locus_tag="PKY68_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOH24021.1"
                     /translation="MNEKILFLEQVDPSVFCGVNNSHIKLLKDLYPKLRIIARGNVIK
                     ILGEEAEIARFEQLINHLQDYLNKYNLINQDVITQLNQGKPLFEPKQENLILYGVNGK
                     PILARTINQQKLVKAYENNDLLFAVGPAGTGKTYTAIALAVRAFKNKEVRKIILSRPA
                     VEAGEKLGFLPGDMKEKVDPYLQPLYDALEDMIPPFKLREYMENKQIQIAPLAFMRGR
                     TLNDAVIILDEAQNTTGLQIKMFLTRLGLNAKMIVTGDLTQIDLPAAATSGLVESLRV
                     LNGIEGIGKVEFDVRDIVRHKLVQHIVEAYEDYHQEQKKLKNTRTDRNNPNE"
     gene            5674..6624
                     /locus_tag="PKY68_02920"
     CDS             5674..6624
                     /locus_tag="PKY68_02920"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOH24022.1"
                     /translation="MKQALVKTDFNFDGQISVYHGKVRDVYNIEDELLVMVVSDRISA
                     FDVVLPKGIPYKGQVLNQIAAKFLDATSDIVKNWKIAVPDPMVTVGVKCEPYEVEMVI
                     RGYLTGHAWREYKSGKRSLCGIQLPEGMKENERFPEPIITPTTKAKEGHDEDISREEI
                     IASGLVGKAEYEKLEEYTRAIFKRGTEMAAQMGLILVDTKYEFGKKDGEIILMDEIHT
                     PDSSRYFYADGYEAKLAANLPQKQLSKEFVRQWLIENGFQGKSGQQVPYMSDEYCRSV
                     SERYIELYEKVTGENFVRAETEDVKARIERNVTQFLKQYR"
     gene            6627..7796
                     /locus_tag="PKY68_02925"
     CDS             6627..7796
                     /locus_tag="PKY68_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOH24023.1"
                     /translation="MSDQRYQLRGVSAGKEDVHRAIKNIDKGLYPKAFCKIIPDILGG
                     DPAYCNIMHADGAGTKSSLAYLYWKETGDLSVWKGIAQDALIMNIDDLLCVGACDHIL
                     VSSTIGRNKHLIPGEVISAIINGTEELLEELRSMGLGVYSTGGETADVGDLVRTIIVD
                     STVSCRMKRADVIDNANIKGGDVIVGLASFGQAVYEKSYNSGMGSNGLTSARHDVFAH
                     YLAQQYPESYDPNTPEKLIYSGQLRLTEPVPGTGMTAGQLVLSPTRTYAPVIKAVLNE
                     LRPRIHGMVHCTGGAQTKVLHFVENKRVVKDKLFPLPPLFKLIQEQSGSDWKEMYKVF
                     NMGHRMEIYLDPSDAERVIEIAAGYGVEAQIIGRIEEARANQLIIESEFGRFEYY"
     gene            7883..8968
                     /gene="prfA"
                     /locus_tag="PKY68_02930"
     CDS             7883..8968
                     /gene="prfA"
                     /locus_tag="PKY68_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOH24024.1"
                     /translation="MTEHSLLDRLDWLQSRFEEVSTLIASPDAVSDMKRYVRLNKEYR
                     DLEQIVLARREYLQLINNINEAKALLEHESDAELREMAREELNKETARQPQLEEKIKL
                     MLIPADPQDSKNAIVEIRGGTGGDEAAIFAGDLFRMYSKYCESKNWKVEVTNSNPGTS
                     GGYKEIIFTVSGEQVYGTLKYESGVHRVQRVPLTETQGRVHTSAATVAVLPEAEEFDV
                     ELNESDIKVDTFCSSGPGGQSVNTTYSAVRLLHIPTGVVVQCQDERSQLKNKAKAMNE
                     LRSRLYDMEYQKYLEEISSKRKTMVSTGDRSAKIRTYNYPQARITDHRINLTMYNLSA
                     VLNGELQEIIDQLIIAENAERLKAGGY"
     gene            8981..>9129
                     /locus_tag="PKY68_02935"
     CDS             8981..>9129
                     /locus_tag="PKY68_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOH24025.1"
                     /translation="MNRQEIISQIKTKATFLCIGLDPDIQKIPSHLLKGEEPLFAFNK
                     AIIDAT"
BASE COUNT         2621 a         2302 c         2095 g         2111 t
ORIGIN      
        1 cgaagaggtt gttgtagatt tccctattgc tgtgcctcaa aaagaacaag ttgacgaagc
       61 tgctccggta gaaaaagccc cggcagaaca agttagcgac accgccaaaa ccgaagaaga
      121 acgcctgctg gaacagttag ctgccgaaac cgaaatccaa acggacgaac aaagcctgga
      181 agaatggaaa agggaatatc ccttgtttgc cgtattgcag attaaccaat ctcagcaagg
      241 tattggtgcc ggcccctgcg taggttatgc tctgggacgc gatactgcca aagtaaacga
      301 atacctcaac agccgcaacg taagggaatt gttgccccgc gatgtgaaat ttttatggtc
      361 ggtcaagccc atcaacgaaa aaacacctct ttttgagctg atcgccataa aagtgaccaa
      421 tcgtgacggt cgcgccgccc tcgaaggcga tgtgatcacc gatgccaggg acgacttcga
      481 acaaatgagc ggctcagccg tggtttccat gtcgatgaat gccgaaggtt ccaaaacctg
      541 ggctcgcatt accaaagaca acatcggcaa gtgcgttgcc attgttctgg ataattacgt
      601 gtattcctac ccccgggtca acaccgaaat caccggcggc tcctcgcaga ttaccggaaa
      661 ctttacctcg gccgaagcca aagacctggc caacgtattg aagtcgggaa aaatgccggc
      721 tcctgcccgc atagtgcaag aagacatagt aggtccttcg ctgggtaaag aagctataaa
      781 gtcggggttg atctcttttg cgattgcttt cgcaattgtg ctgatctaca tgatcttttt
      841 ctacggaatc attcccggtc tggttgccga cttcgccctt ttgtgcaaca tcttcttttt
      901 gatgggtata ttggcctctt tccaggcggt gctgacactg cccggtatag cgggtattgt
      961 gctaaccctc ggcatggcca tcgacgccaa tgtgcttata aatgagcgca tcaaagaaga
     1021 actggcagcg ggtaaaagca tcaaaaaagc cttgcccgac ggatacaaga atgccttctc
     1081 ggccatcttc gactccaacc ttaccacctt gctgaccggt ataatactct ttatactggg
     1141 aacgggaccc atcaaaggtt ttgccactac tttgagcatc ggtatcctga cttcgttttt
     1201 cagtggagtc tttattaccc gcatcatctt tgaacgtctg ctggccaagg aaaaactaaa
     1261 taatatcact tttaccacca agttatcgaa aaatatttta aggaatcccc aagttaattt
     1321 tttgggcctg cgcaagaaag gctacatcgt tgcgggagtt ttggtcttgg cgtgcatttt
     1381 gtcgtggagc acacgcggat tgagtcaggg catcgacttt tcggggggtc gcaattacgt
     1441 ggttcgcttt gatcagcccg tttctacgca agacgttcgc aacagccttg atggcatttt
     1501 cgaggatgcc tcgctttcgg taatcactat cggctccgaa aaccaggtca gggtaagtac
     1561 caactacaag ataaatgaaa cctacgatga actggatgcc gaaatcgaca gcatactgtt
     1621 caccggctta aaagactact tgcccgaaaa taccggtttg gaatcctttg tcgaagacta
     1681 catagtatct tcgcaaaaag tcgggcctac cgttgcttct gacattaaga tttcggctat
     1741 atggtcggta gtggctgctc tgctggtaat cggcttgtac atcctgttgc gtttccgcaa
     1801 cgtggccttc agcgtcggtg ttgtcgcttc cttagccttc gttaccatat ttattattgg
     1861 cttgtattct atgctgtaca gcgtgatgcc tttctcgttg gaaatagatc aaacctttat
     1921 cgctgccatc ctgacggtga ttggttatgc cgttaacgac tcggtggtga tcttcgaccg
     1981 tatccgcgaa acaacgggcc tttatcccaa gcggcacaaa cgactcacca tgaactcggc
     2041 tatgaatacc accttgttgc gtactttcag caccgcattt agtaccgctc tagtgctgtt
     2101 ggccatcctt ctttttggag gagacaccat ccgcggtttt gcctttgcca tgttgatagg
     2161 tgtggtattc ggaacgctcg gaacgctctt tattgcctct cccgttgctt acgatattca
     2221 gcgcaagaga gaaaaattca gtgatgaagt cacagaagaa gaatagagaa aacccttccc
     2281 tgtccattcc aataccagcc gttgattgaa gcaatctctg tagcccgcaa aagcctcctc
     2341 tcccaggaaa tcgggcagcg aagaatagac aagcaaaaaa ttgtttccgc tctgaaagtt
     2401 gtattcataa aaatacaagc tgttcaattc ttcaacgctc atatcctggg cgtgaccctt
     2461 ttgttgcagg caatcctgat ttaaaaaaga agcagcgctc aaacgaggct tagccaatgt
     2521 gtcgataggc tgccaatcga gattgtaaaa ttccaggtca ctgctggcgg ccggcgcctt
     2581 gaccgtgttg acaacagcca gtatccggct cccgttttta aggggtaaca actttaactg
     2641 cacattgctc gcagccgagg tctgcaacat taagtaatct tcggtgagta ttttcaattc
     2701 catctctgaa cctaataaac caagcattct cgctgttttg ccgctgttaa agaaatcgac
     2761 catatccaac ttcatggcgg tgttcagccc cggcagcaaa tcggcgggca tatctttgaa
     2821 acattccact acacttcgac tccaaacgcc cggtacggtg aagcaggtaa ttaacaacag
     2881 taaaacgact tttctcatac ctaaacaacg attacttaga gcctaaatac aggcaacaca
     2941 ttccaataaa aacgcccaac aaaatccagg ctttggcctt aatgtttttg tctttaaaga
     3001 acaaagcccc taccacaaaa ggaattaaca cccctgaacg gcgtatgggc gaaatcaccg
     3061 aaatcaatgc tcccggcctg ctcagggcgt aaaagtacaa aaagtctgcc aggcagagaa
     3121 acacggctaa acctactatg gaccagcgaa agtcgaaggc ctgttccttg cctctttttc
     3181 gccggtgcat atagatgagc aacaggggca acatgatgaa tatctgataa atggtgtacc
     3241 aaacctgcac agtcatgcgg tcgaaattca ccatcatata tttatcgtag agtccgctga
     3301 ccgatccgat caacacggat aagaacaaac accagatcca gggattgcgc ctaaaagaaa
     3361 taccttcttt tttgcccaca taagagtaca gaaaaatact gatgattgaa accagcaccc
     3421 ccagccattg ccaaaggttg agccgctccc caaaaaccat aaaagcaccc atcagtacaa
     3481 aaaccggctg agtggattta atgggagata caatggttaa aggtagattt ttgatggcat
     3541 aataggcagc ggcccacgag cttaaaacga taaacgattt aagtaaaata aaaagatgtg
     3601 ttttcgcgtc tgcccgcggc acaaaaaaca aggtgttttc gaggctttgc ggagcccact
     3661 ccgaaagcag caacaaaggc agaaaaaaca gggaacaaaa aaccgtggtc aaaaacaaga
     3721 ccggtacaac agcattttcc tttaacgaca gttttttata tacttcgtaa acccccaaca
     3781 agagggaaga agcaaaagta gcccacaacc acataagtca aaaatcaacc aattaaatac
     3841 aaatcatcac tatatttcac ctctgtcaaa tacagggcct gtgccggcac ggatgtgcct
     3901 gccctgcaac ggtcgcgggc ctcgataata cggcaaaatc cctcctcgtc tatcctgccc
     3961 ttgcctactt cgagcaaact acccactatg gctcgtacca tattgcgcaa aaaacggtcg
     4021 gcttgtatgg taaaaatcag ctcactcccc cggcgttccc aacgagcatg tgttaccttg
     4081 caaataaagg tcttcacgtc ggtatgcact ttgctgaagc tggtaaaatc gctgtactcc
     4141 aacaaacggc cagcagcctt gttcatagcc tccacatcgg gttcgtaata caaacgaagg
     4201 ctgtaatctc tcaggaaagg atctttttgc agtgttattt tgtactgata agttctcgag
     4261 atggcatcga agcgggcgtg agcgccgggc cgtacccttc ggagggaacg aacggcaata
     4321 tcggccggca gcattcggtc cagtttatga gccgttgcat ccggattttc gatgggatgg
     4381 gccgtatcga aatgcgccgt catagctaag gcgtgtacac cggcgtcggt gcgtcctgca
     4441 gcaataatat ccaggtcttt ttcgaataaa gtgcgcagca cagaggtcat aacttcttgc
     4501 acgctaatgg cattgggttg tatctgccat ccgtgataag cgctgccgtc gtaagaaaaa
     4561 ttcagaaaaa aacgttgcat taccgggaga ggaaaaattt ggctgcaaag ttagtcttta
     4621 ttttttattc caatcgaatt gagtactttt gcgcaaaatg ttaggccccg ggtgaatgaa
     4681 cgaaaaaatc ctgtttctgg aacaagtaga cccctctgtg ttttgcggag tcaacaacag
     4741 tcatatcaaa ctactcaaag atttatatcc caaactacgt atcattgccc gcggaaatgt
     4801 gattaagatc ttaggcgaag aggccgaaat tgcccggttt gaacaactaa tcaatcactt
     4861 gcaggactac ctgaacaaat acaacctgat caaccaggat gtcattaccc aactcaacca
     4921 gggcaagccc ctttttgaac ccaaacagga aaatcttatt ctttacggtg tcaacggcaa
     4981 acccatcctg gccagaacca tcaatcagca aaagctggtt aaagcttacg aaaacaacga
     5041 cttgttgttt gccgtagggc cggccggcac aggtaaaacc tatactgcca tcgccctggc
     5101 cgtaagagct tttaaaaaca aagaagtacg taaaattatc ctgagccgtc cggcggtcga
     5161 agccggtgaa aaactgggtt ttctgccggg cgacatgaaa gaaaaggtcg atccctacct
     5221 gcaaccgctt tacgatgccc tcgaggatat gattccgccc tttaagctca gggaatatat
     5281 ggaaaacaag cagattcaga ttgctcctct ggcttttatg cgcggaagaa ccctgaacga
     5341 cgccgtcatc attttggacg aagctcaaaa caccaccggc ttgcaaataa aaatgtttct
     5401 tacccgcctg ggcctgaacg ccaaaatgat agtcaccggc gacctcaccc aaattgacct
     5461 tccggcagca gccacatcgg gtttggtcga gtcgctcaga gttttgaacg gtatcgaagg
     5521 catcggcaaa gtggagttcg atgtgcggga catcgtcagg cacaagctgg ttcaacacat
     5581 agtggaagcg tacgaagact accatcaaga acaaaaaaaa ctaaaaaaca cccggaccga
     5641 ccggaataac cccaacgaat aaactctagc tttatgaaac aggctttggt aaaaacagac
     5701 ttcaattttg acggacaaat atctgtttat cacggaaaag tccgtgacgt atacaatatc
     5761 gaggacgaat tgttggtaat ggtggtcagc gaccgcatat ccgccttcga cgtggtactg
     5821 cccaaaggaa tcccttacaa gggacaggtg ctcaaccaaa ttgccgctaa atttctggac
     5881 gccacctccg atatcgtcaa aaactggaaa atagccgtgc ccgaccccat ggttactgta
     5941 ggtgtcaaat gtgaacccta cgaagtggaa atggtgatac ggggctatct cacgggccat
     6001 gcctggagag aatacaagtc cggcaaacgc agcctgtgtg gcattcaact acccgagggc
     6061 atgaaagaaa acgaacgttt tcccgaaccc atcatcaccc ccaccaccaa agccaaagag
     6121 gggcacgacg aggatatttc cagagaagaa atcatagcct ccggcctggt cggcaaagcg
     6181 gaatacgaaa aattggaaga atatacccgg gctattttta aacggggcac cgaaatggcc
     6241 gcccaaatgg gcctgatcct ggtggacacc aaatacgaat tcggcaaaaa agacggcgag
     6301 atcattctga tggacgaaat ccacactccg gattcttcac gctattttta tgctgatggt
     6361 tacgaagcaa agctggcagc caacctgcct caaaaacaac tttccaagga gtttgtccgc
     6421 caatggctca tcgaaaacgg gttccagggt aaaagcgggc agcaggttcc ctacatgagc
     6481 gacgaatact gccggagcgt ttcggagcgc tatatcgaac tttacgaaaa agtgaccggc
     6541 gaaaactttg tacgtgcgga aaccgaagac gtaaaagccc gtatcgagcg caatgttacc
     6601 caatttttga aacaatatcg ttaattatgt ccgaccagcg ctatcagctc agaggggttt
     6661 cagccggcaa agaagacgta caccgggcta ttaagaatat cgacaagggt ctttatccca
     6721 aggccttctg caagataatt cccgatatac tgggcggcga tccggcttat tgcaacatta
     6781 tgcatgccga cggagccggc accaagtctt cgctggccta cctctattgg aaggaaaccg
     6841 gcgatctttc cgtttggaaa ggcattgccc aggacgcctt aataatgaac atcgacgacc
     6901 tgctttgcgt cggggcctgc gatcatatat tggtttcttc taccataggc cgcaacaagc
     6961 atttgattcc gggcgaagtc atttcggcca ttatcaacgg caccgaagaa ttgctggaag
     7021 aactccgctc catgggcctg ggtgtttatt ccaccggagg cgaaacagcc gatgtgggcg
     7081 acctggtgcg caccattata gtggacagta ccgtaagttg ccggatgaaa cgagccgatg
     7141 tgatcgacaa cgccaacatc aaaggaggag atgtcatcgt agggttggcc tcgtttggac
     7201 aggctgttta cgaaaaaagc tacaacagcg gcatgggaag caacggactc acttcggcca
     7261 gacacgatgt gtttgctcat tacctggctc aacaatatcc cgaaagctac gaccccaata
     7321 cacccgaaaa attaatttac tccggacagc tcagactgac agaacctgtt cccggcacgg
     7381 gaatgactgc cggacaactg gtgctttccc ccacccggac ctatgctccc gttatcaagg
     7441 cagtactgaa cgaacttaga ccccggatcc acggcatggt gcattgtacc ggaggtgctc
     7501 agaccaaagt actgcatttt gttgaaaata aaagggtggt caaagacaag cttttcccct
     7561 taccgcctct attcaaactg atacaggaac aatcgggcag cgattggaaa gaaatgtaca
     7621 aagtcttcaa catggggcac cgcatggaaa tctatctcga tccttcggat gccgaacgag
     7681 ttattgaaat agccgccggc tacggcgtcg aagctcaaat aatagggcga attgaagaag
     7741 ctcgggccaa tcaattgata atcgaaagtg aattcggccg tttcgaatat tattagccgg
     7801 aatttgtaac gaaaacagcc tttagcacat ctatgggctg gcacccgcac aactttttct
     7861 tttttttctt ttaagcaaat aaatgaccga acattcctta ctggacagac tcgactggct
     7921 acaaagccgt tttgaggaag tatccaccct gattgcctct cccgacgcgg tttcggatat
     7981 gaaacgctac gtcaggctca acaaagaata cagggatctg gaacaaattg tcctggctcg
     8041 ccgggaatac ctgcaactca tcaacaacat aaacgaagcc aaagccctgc tcgagcacga
     8101 aagcgatgcc gaattgcgcg agatggcccg cgaggagctg aataaagaaa cagccagaca
     8161 gcctcaactc gaggaaaaga taaaactcat gctgattccc gccgatcctc aggacagtaa
     8221 aaacgccata gtcgaaatcc gcggaggtac cggcggtgac gaagctgcca tctttgccgg
     8281 cgatttattc aggatgtaca gcaaatattg tgaatccaaa aattggaaag tcgaggtaac
     8341 caactccaac ccgggcactt cgggaggcta caaggaaatc attttcaccg tcagtggtga
     8401 gcaggtctac ggaacgttga aatacgaatc gggggtacat cgcgtacaaa gagtgcccct
     8461 gaccgaaacc cagggcagag tgcacacttc ggcagccacg gtggccgtgt tgcccgaagc
     8521 cgaagaattc gatgtggaac tgaatgaatc cgacattaag gtggatacct tttgttcttc
     8581 cggacccggc gggcagtctg tcaacactac ttattcggcc gtccgcctgc tgcatattcc
     8641 caccggtgtc gtggtacaat gccaggacga acgttcgcaa ctgaaaaaca aagccaaagc
     8701 catgaacgaa ttgcgctcgc gcctttacga catggaatac caaaaatacc tggaagagat
     8761 ctcttcgaaa cgtaaaacaa tggtttccac cggagaccgt tcagctaaaa tccgtaccta
     8821 caattaccct caagcccgta tcaccgatca caggatcaat cttacaatgt acaacctgag
     8881 cgccgtactc aatggtgagt tgcaggagat tatcgatcag ctaatcattg ccgaaaacgc
     8941 cgaacgcctc aaagccggag gttattgata cccatatatc atgaacagac aagaaattat
     9001 ttcccaaatc aagaccaagg ccaccttttt gtgtatcggc cttgatcccg acatacaaaa
     9061 aataccctca catcttctga agggggaaga gcctttattc gctttcaaca aagccattat
     9121 cgacgccac
//
LOCUS       DAOSGL010000055         8907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6686_length_8907_cov_4.322752, whole genome shotgun sequence.
ACCESSION   DAOSGL010000055 DAOSGL010000000
VERSION     DAOSGL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6686_length_8907_cov_4.322752"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6024)
                     /locus_tag="PKY68_02940"
     CDS             complement(<1..6024)
                     /locus_tag="PKY68_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24026.1"
                     /translation="MNIPSQIKQIKKAALVGLCLLSMLFFAATSIKTYADVIVHIGGD
                     VYGGGRNGAVGTGFNVTVFLTQDEVDAENNEHLTADSLGRNPGDAGYVTTYEEGYIPV
                     SIGDINNNAAFDKVKLAGDTPQLITDVTIKSGSVRTVFGGGENGRVYGGTTVNVSGGV
                     VGDSIWKGTIHGGVFGAGDGESAHVFGHDRVTITGGTIYNNIYGGGNQAHLVGSTTVI
                     LRGGAVAGDVFGGSRMAQIFGYSFVNIDGANATDDLIIKTIYGGNDICGTISSSNNWE
                     WTKAENLSAPFTLKIPSINNTWNAFILSSASSTENIFIGKVYGGGNGDYNYVDSTLSL
                     IDQPGETPTYKQFTGLSLPEIDKIYLELNGGTFGYVFGGGNAATVRNSVDICLDNQTE
                     TPAWIPTGYIPVFEAELGPIFIDTGNPSFKTVEYQFDRVFGGNDQIAMGIRPNWYLNK
                     ADINNLYSGGNAGDMTFFDNTDPGNIKGGILLALTSDSLTVNNVYGGCRMADVNPAKN
                     EFPAETINGVEFPANHAARVLITAGTINNVYGGNDISGIVYGGNALEILSSIKGDVYG
                     GGNGSYPYTDSQDYVDAHPEYADYLYDGGSNSIDSLNHHRPNAESVWLHVAGVSEEKR
                     TYIGGALYCGGNSAALHSETISSTAKAELKIGAYATIDKVFLGSNGINMIAPELIDLY
                     KNAANFSSIDLTGGFADYMKGVEVAIEPTLSFDDLTGKEYTTYIGSLYFGGNVGSMSA
                     PGTFDMDFDEPIVIYDRIVGGCNDANVDTIFAENGIDVLLTPYYKGGLLTRPESEDDP
                     KVRININSGNNGNTVRLLPKKLTYDGSTYQLEWNITGTGADAKLVGGNIYGGCYNSGY
                     INGGVEINILGKATDTLQVFRTPTLENSGIEKESQLAKVFTKALSAFGGGYGEDSEIW
                     GSTTINIDSEGYITQVYGGGEKGIIGKTVAGTDANGNPNIAADGIYGTLINLRGGHVD
                     KIYGGGYAGLVSGNVRVNLDGGHAGRVFGGACNADINGYSEVFIGLEGFPEITNIYGG
                     NDFGGHIYSKGTHAGYGSAQKESNAYVEFFRGNIGNIFGGSYGDYVYSIDSSPYYGYD
                     QPCFEASFVHIKNSGTAANFIGAIYGGSYGHPEELYQLMQFNSYVLINAPALNLPGTD
                     IFGGGLATGFRTIDSIEGTSTVDLYQGTFRNVHGGSLNCGIVPITTINVPEESTVHVN
                     TLFGGGLGSTNNRRCDVHKAYIEYHGSNARVDNAIYGGNCSYRRTIESYVNISAAVYN
                     TSNQLVDIYGGGYGEATWAECTHVNLQNGAEVGNVYGGGNQGKVYNSAAVIAQGFPQS
                     TPKSSYLEGEYNTNINIFEGALADSCVYGGGYGAQATVTGTTGVNLLGGAVELDIYGG
                     GENGHVINDTDLGTTSDVSTNVNLIGGTVRNAYGGGYNGNVGTVALPNGETHVNIGIT
                     DSTLFTNGVPAIKRSVYGGGYRGKVYGTTKTNMYNGYIGYVYNKNGSDDIGTDDFNEK
                     YEENLNYPNSTLNLLEENGNLFGGGYGEDAITDTTIVTFYGGNIRNSLYGGGEISSVG
                     RGTDENGIIPNTHSDFRAGATNVYMYGGRVHRNVFGGGRGYAIDSYGNTETGVKGYSD
                     GYVFGTTSVYIHRGSVGTHFTVLDGDGNVFGGGNIGYVYSGKGTKSLLDGYYYDGDTL
                     TEDCKVIISPKCLVTTAFDTYQVGDYVDADFMNTLPNNSPIWGKMDDLGINIGNAVFA
                     GGNVSRGSDMVYANTETVFGNATASVVDVFDKDFITIGEDGIGGLYGDGNLTLVDGYR
                     ELNITNYGTDYYGLDASLTFEQYQDLNKRERAYYELKYQSKNKHTYSFYESKLLHTYE
                     YTIGEGDEAVTVRVTYKKGQKITEAEWSSLSAEEQNNWRQSARTYEEYEQITENEYDL
                     MDSAEKENWELYGFCTLYLGRMMNTIQRADFCGVFGSRIVMRGAQDRVTSVVDYTNYT
                     INRVDEVSLNQEHQMVGSENMSHGNYFGIYSVVNYLGALTSDVEFNDIRK"
     gene            complement(6038..7171)
                     /locus_tag="PKY68_02945"
     CDS             complement(6038..7171)
                     /locus_tag="PKY68_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HOH24027.1"
                     /translation="MRVTIKSIAGSIKSLTAILAAATLILTSCRDDAASTVTLYPVVS
                     SNVETVVETRALSSDYKAFEVVGTRIQAYALAFNKYIDIRVSGNDADGIFLRKIPGWT
                     STLEVTHDLRYRVYAYSPAPFPAETISFDYNGGTPKLTFTGLSVITETDPMASVAAAG
                     GTGDTPATDFSEGSFSIGVVNILTSPKTKVWLAMNHIYSKATISFVVNAGYDAIRTIV
                     LKNATVSIPTGILPGLSTYTFAKGSSPAVATWSSSDLNGDAKTIDLMFGPTAEPDLLD
                     LSGDPEPQPLNQVTLSASSKKFAWFCFLPKSKPALTLKVVYDIYDKEGNLIRADQTVT
                     NANILANLSSVAAGNNYNITVRVNPTYLYQLSDSDASLELIIE"
     gene            complement(7194..8291)
                     /locus_tag="PKY68_02950"
     CDS             complement(7194..8291)
                     /locus_tag="PKY68_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HOH24028.1"
                     /translation="MRNNINIRKMFRPVVLTLTAIAVVIPTACCRHEVPAENAILMSA
                     RQDETTRAVINEKNDLGRQSVSDGAGFGVYGYKTANYNVVGEEIETQVFSNTKVTATG
                     SDDNYNWSYSPLKYWDAVTYYRFVAYWPHTSETPSVDEEYVSHNETNHIITLRNLPCW
                     QEVDSSENDWLISTSRKYASQYITQDNGYVRFHFQHLLARIEIKAWYWGREEKKPEIT
                     ELSIGNSTYKVPADTAKTTVSRDYATALSSNSWGSITYADSMKLADTTLINAYSDYED
                     EAPENIDLVCEWLVVPFSATGCPLTLRYKIDGVSFDPVTLDASLGAIESGKKYTLIFK
                     FNTVTNSLVLEELLVKDWVDSGNFNKDVHNW"
     gene            complement(8386..>8907)
                     /locus_tag="PKY68_02955"
     CDS             complement(8386..>8907)
                     /locus_tag="PKY68_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24029.1"
                     /translation="NGDCVVDTELEREIEVVPGVVTDPDDPNYPENPNIDYNGFVDNY
                     QNPSGYLITDNEGEYWIDEDGDGVGDIKILWMDIDGDGKLEGIADKNGDGILTIEDSY
                     DNDGLDYNGNPNDYDVIMVDGDGDGTAETELEKEITIVPGDLPDVEIEDSAIEFSAEV
                     DNWIDEYDADYKM"
BASE COUNT         2128 a         2250 c         1990 g         2539 t
ORIGIN      
        1 cttgcgtata tcgttaaact caacatcgct ggttaatgcc ccaaggtagt tgaccacact
       61 gtatataccg aaatagttgc cgtgcgacat attttctgag ccaaccatct ggtgctcctg
      121 gttaagcgaa acctcatcca cacgattgat agtatagttg gtataatcca caaccgacgt
      181 tacgcggtct tgtgctccac gcataacgat acgactaccg aatactccgc agaagtcggc
      241 acgctgtatg gtgttcatca tgcgacccag atacaaggta cagaaaccgt atagctccca
      301 gttctctttt tctgcgctat ccataaggtc gtattcattc tccgtgattt gttcgtactc
      361 ctcataagtc cttgccgact gcctccagtt gttttgctct tcggcggaca aactgctcca
      421 ctcggcctca gtgattttct gacctttctt atatgtaact ctcacggtta ccgcctcgtc
      481 gccctcgccg attgtatatt catacgtatg aagcaatttt gattcgtaaa aactgtacgt
      541 atgcttgttt ttcgactgat atttaagctc gtaataagcg cgttcacgct tgttgaggtc
      601 ctgatactgt tcaaatgtga gcgatgcgtc aagtccgtaa taatccgtac cgtagttggt
      661 aatgttcaac tcgcggtaac cgtcaaccag agtaaggttg ccgtctccgt aaagaccgcc
      721 tataccgtcc tcacctatag ttatgaagtc tttgtcaaat acgtcaacaa cagaagctgt
      781 cgcattgccg aataccgttt cggtgttggc atataccatg tcggaaccac gcgacacatt
      841 acctccggca aaaacggcat tgcctatgtt tatgcccaga tcatccatct tgccccatat
      901 cgggctgttg ttgggcagtg tattcatgaa atccgcatcc acatagtcgc caacctgata
      961 ggtatcgaat gccgttgtta ccagacattt cggcgaaatt atgactttac agtcttcggt
     1021 aagcgtatcg ccgtcgtaat agtacccgtc cagaaggctt ttagtaccct taccgctgta
     1081 gacatagcct atgttgccgc ctccaaacac attaccgtcg ccatcaagca cagtaaaatg
     1141 ggttcctacg cttccccggt gaatatatac acttgtggtt ccgaacacat aaccatcgga
     1201 gtaacctttg acaccggttt cggtgtttcc ataggagtct atggcgtatc cgcgcccgcc
     1261 gccgaacaca ttgcggtgca cgcgcccgcc gtacatatag acattggtgg ctccggcccg
     1321 gaagtcgctg tgcgtattag gtataattcc attttcgtcg gttccgcgtc cgacggacga
     1381 aatctcgcct ccgccgtaaa gactgttacg tatattacca ccgtagaaag tcactattgt
     1441 ggtatcggta atggcatcct cgccgtatcc gccaccaaac aggtttccgt tctcttccag
     1501 cagattaaga gtgctgttcg gataattcag gttctcttca tatttctcgt taaagtcgtc
     1561 agtcccgata tcatcggagc cgtttttatt gtaaacatag cctatatagc cgttgtacat
     1621 atttgtctta gttgtgccat agaccttgcc tctgtacccg cctccgtaaa cactgcgttt
     1681 aatggccggt acgccattgg tgaaaagggt ggagtcggtt atgccgatat ttacatgagt
     1741 ttcaccgttt ggaagagcaa cagtacctac atttccgttg tatcctccac catacgcgtt
     1801 acgcaccgtg ccgcctataa gattgacatt ggtagagaca tccgatgtgg tgccgagatc
     1861 tgtatcgttt atgacgtgcc cattttcacc tcccccataa atgtcgagct ctacagcacc
     1921 gccaagaaga tttactcccg tggtaccggt tacggtggcc tgcgctccat aacctccgcc
     1981 gtacacacaa ctatccgcca aagccccttc gaatatgttt atattggtgt tgtactcacc
     2041 ctcaagataa ctggatttag gtgtgctctg cggaaatcct tgtgcgataa cggcagcact
     2101 attatagact ttaccctgat tacctcctcc atatacattg cctacttctg ctccattctg
     2161 taaatttaca tgagtgcact cggcccaggt tgcctcacca tatccgccgc catagatgtc
     2221 aaccagttgg ttgcttgtat tgtaaacagc agccgatatg ttaacatagc tttcgatagt
     2281 gcgtctgtag ctgcaattac caccgtaaat ggcattatcg actctggcat tggaaccgtg
     2341 gtattctatg taagctttat ggacatcaca tcttctattg ttagtgctgc caagacctcc
     2401 gccgaagagt gtgttcacat gaacggtcga ctcctcaggc acgttgatcg tagtgatggg
     2461 aacaataccg caattcaatg aaccgccgtg aacattacgg aaagtaccct gataaaggtc
     2521 aaccgtactt gtaccttcaa ttgaatctat agtcctgaat cctgtggcca aaccgccacc
     2581 aaagatatcg gttccgggca gattcagggc cggagcgttt ataagcacat aactgttaaa
     2641 ctgcataagt tgatacagct cttccggatg tccgtagctg ccgccgtaaa tagcgccaat
     2701 gaaattagcc gcagtaccgg agttctttat atgaacgaac gaagcttcaa agcatggctg
     2761 gtcataaccg taatacggcg agctgtcgat tgagtaaaca taatcaccgt atgaaccgcc
     2821 gaatatgttg cctatatttc cccggaaaaa ttcaacatag gcgtttgatt ctttttgtgc
     2881 gctgccgtag ccggcatggg ttcctttgct gtaaatgtgc cctccaaagt cgttaccgcc
     2941 gtatatattg gtaatttcag ggaatccttc caatcctatg aatacttcgg aatatccgtt
     3001 gatgtcggca ttgcaggctc cgccgaatac tctgccggca tggcctccgt cgaggttgac
     3061 acggacattg cccgatacca gacctgcata acctcctccg taaatcttgt caacatgacc
     3121 gcctctaaga tttattagtg tgccgtatat tccgtcggca gcaatattag gattaccgtt
     3181 ggcatcggtc ccggctactg tctttcctat aatacccttc tcaccgccgc cgtacacctg
     3241 cgttatatag ccttccgaat cgatattgat ggtggtgctg ccccatattt cggaatcttc
     3301 accgtagccg ccgccgaaag cgctcagggc cttggtgaaa actttggcca actggctctc
     3361 tttctctatt ccggaattct ccagcgtcgg agtacggaac acctgcaagg tatctgtggc
     3421 tttgcctaaa atattgatct ctacaccgcc gttaatatat ccgctgttgt agcagccgcc
     3481 gtatatgttg cctcccacca gctttgcgtc tgcaccggta ccggtaatat tccattcaag
     3541 ctggtatgtg ctgccgtcat aggtgagttt ttttggcaac agtctgactg tgttgccgtt
     3601 attgccgctg ttaatattga ttctgacttt ggggtcgtct tcactttcag gtcttgttaa
     3661 gagtcctccc ttatagtacg gagtgagaag cacgtcaata ccgttttcgg caaatattgt
     3721 atcgacattt gcatcattgc accctcccac aatacggtca tagatgacaa tcggttcatc
     3781 gaagtccata tcgaaggtgc ccggcgccga catactgccg acattaccgc caaaataaag
     3841 cgaaccaata taggtggtgt attctttgcc tgtcaggtca tcgaaagaga gggtaggttc
     3901 tatggcaact tcgacgcctt tcatataatc ggcgaatccg ccggtcagat cgatactgct
     3961 gaaattcgcc gcgtttttgt ataggtctat caattccgga gcaatcatgt taattccgtt
     4021 gctgcccagg aagaccttgt cgatggtggc gtaggcgcca attttaagtt cggcctttgc
     4081 ggttgacgat attgtttcac tgtgaagtgc ggcgctgtta cctccacaat aaagcgctcc
     4141 tccaatgtag gtgcgtttct cttccgatac gcctgccaca tgaagccata cgctttcggc
     4201 gttgggacga tgatgattca gggagtctat ggaattgctg cctccgtcgt acaggtaatc
     4261 ggcatattca ggatgcgcgt caacataatc ctggctgtca gtgtaagggt atgaaccgtt
     4321 gcctccaccg taaacatcgc cctttatgct gctgagaatt tcaagcgcat tacctccgta
     4381 aacaatgccc gatatatcgt tacctccata tacattgttt atagtacctg cggttataag
     4441 aacgcgggcc gcatggttgg cgggaaattc aacgccattt atggtctccg cagggaactc
     4501 atttttagcg ggattgacat cggccatacg gcatccgccg taaacattat ttacagtcag
     4561 gctatcgctg gtcagagcaa gcaggatacc tcccttgata ttacccggat cggtattgtc
     4621 gaagaaggtc atatcgccgg cgttgccgcc cgaatagaga ttgtttatat cggctttatt
     4681 cagataccag ttgggacgta tgcccatcgc aatttggtca ttacctccga atacccggtc
     4741 aaactgatat tcgacagtct tgaaggaggg atttcccgtg tcaataaata tgggacccaa
     4801 ctccgcctca aacaccggaa tatatcctgt gggtatccat gccggagttt cggtctggtt
     4861 atccagacag atgtcaacgc tgtttcttac ggtagccgca ttgccgccgc cgaatacgta
     4921 accgaaggtg ccgccgttta attcaagata tattttgtcg atctccggca ggctcagtcc
     4981 tgtaaattgt ttgtatgtgg gtgtctcgcc cggctgatct ataagcgaga gcgtactgtc
     5041 aacatagtta taatcgccgt tgccgccgcc atagactttt cctatgaaga tgttttccgt
     5101 acttgaggcg gacgaaagta tgaacgcgtt ccaggtgttg ttgatagacg gtatcttaag
     5161 tgtgaaagga gcactcagat tttccgcttt tgtccattcc cagttgttac ttgaagagat
     5221 tgtaccgcaa atgtcattac caccgtaaat tgtttttatt atcagatcgt ctgtggcatt
     5281 agcgccgtca atattcacaa agctgtaacc gaatatctgg gccatacgtg aaccgccaaa
     5341 cacatcaccc gctacggcgc cgccgcgtag aataacagtc gtactgccaa ccagatgagc
     5401 ctggttgccg ccaccgtaaa tattgttgta gatggtgcct ccggtaatgg ttactctatc
     5461 gtgcccgaac acatgggcac tttcgccgtc tccggcgccg aaaactccgc cgtgaatggt
     5521 gcctttccag atgctgtcgc caaccacacc tcccgaaacg tttaccgtag taccgccgta
     5581 aacacgtccg ttttcaccgc cgccgaacac cgtgcgtacc gagccgcttt tgatggttac
     5641 atctgttatg agttgaggag tgtcaccggc aagtttaacc ttgtcgaaag cggcgttatt
     5701 atttatgtcg cctatggaga cgggaatata cccttcctca tatgttgtaa catatcccgc
     5761 atcgccgggg ttacgcccca aagaatcggc cgtcagatgt tcgttgtttt ccgcatcgac
     5821 ctcatcttgt gttaggaata ctgtcacatt gaatccggtt cccacagcac cgttacggcc
     5881 gccaccgtac acatcgccgc caatatggac tataacgtcg gcatatgttt taatgctggt
     5941 agccgcaaaa aacagcatgc tcaacagaca tagtccgaca agtgccgctt tttttatttg
     6001 ctttatttgg gatggtatat tcataatatc cggttgttta ttcaataatc aattccaatg
     6061 aagcatcgga atcactcagt tgataaagat aagtaggatt aacccttacg gtaatattgt
     6121 agttgttacc ggcagcgacc gaactcaaat ttgccagaat attggcgttc gttacggttt
     6181 ggtcggcacg gataagattt ccctccttgt cgtatatatc gtaaaccact ttcagggtca
     6241 gagccggttt ggatttgggc aagaaacaga accatgcaaa ttttttggac gatgcactga
     6301 gtgttacctg attcagcggt tgtggctccg gatcgccgga aaggtcgagc aagtcaggct
     6361 cggctgtggg tccgaacata agatcaatgg ttttcgcgtc accattcaga tctgaactgc
     6421 tccatgtcgc gacggcaggc gatgagcctt tggcaaaagt gtaagttgat aagccgggta
     6481 gtatcccggt cggtatcgac actgtggcat ttttcagtac gattgttctt atggcatcgt
     6541 aaccggcatt gacaacaaaa gagatcgtgg ctttggagta aatatggttc atagcgagcc
     6601 atacttttgt tttaggggag gtgaggatgt ttactactcc tattgagaat gaaccttccg
     6661 aaaaatccgt tgcaggcgta tcgccggtac cacctgctgc ggcaacgctc gccatagggt
     6721 cagtttctgt aatcacactc agtcctgtaa atgtcaactt cggagttcct ccattataat
     6781 caaacgagat cgtttcggca gggaatggtg caggcgaata agcatagact cggtatctta
     6841 agtcgtgtgt cacttcgagt gtggaggtcc aaccgggaat tttacgtaag aaaataccat
     6901 cggcatcgtt accgcttact ctaatgtcga tatatttatt gaaagctaaa gcgtatgctt
     6961 gtattcttgt tcctacaact tcgaatgctt tgtagtcgga actcagagca cgggtttcaa
     7021 caaccgtctc aacattactg cttacaacag gatagagcgt aaccgtcgaa gcagcatcat
     7081 cccggcacga tgtcaggatc agtgttgcgg ccgccagaat tgcagtaaga ctcttaattg
     7141 agccggcaat tgattttatt gttaccctca taatctccat atacaatcgt tagctaccag
     7201 ttgtgaacat ctttattgaa attacccgag tcaacccagt ctttaaccag cagttcctcg
     7261 agtactaaac tgttggttac cgtgttgaat ttaaatataa gcgtatattt cttacccgac
     7321 tcaatggcgc ctaatgaagc gtcgagagtt accggatcga acgaaacgcc atctatctta
     7381 taccttaagg taagcggaca gcctgtagct gaaaaaggca caacaagcca ttcacatacc
     7441 aagtcgatgt tttccggcgc ttcatcctca taatcggagt aagcatttat cagtgttgtg
     7501 tcggcaagtt tcatactgtc ggcataggtg atagagcccc aactattgct actcaacgct
     7561 gtggcgtaat ctctgcttac agttgttttt gccgtatcgg ccggcacctt ataagttgaa
     7621 ttcccgatgc taagctccgt aatttcaggt ttcttttcct ccctccccca ataccaggct
     7681 tttatctcaa ttcgcgccag cagatgctga aaatggaatc gcacatagcc gttgtcttgg
     7741 gtaatgtatt ggctggcgta tttccggctt gtcgagatca gccagtcgtt ctccgaactg
     7801 tctacttcct gccaacaggg aagatttctg agcgtaataa tatggtttgt ttcgttatgg
     7861 ctaacatact cttcgtcgac cgatggggtt tctgaagtat gtggccagta ggcgacaaaa
     7921 cgataatagg tcactgcgtc ccagtatttc aacggactgt aactccagtt gtaattatcg
     7981 tcgctccccg tagccgttac cttggtattg gagaagactt gtgtctcaat ttcctctcct
     8041 accacattgt agtttgcagt tttgtagccg taaacgccga aaccggcgcc atcggatacg
     8101 ctctgtctgc caagatcgtt tttttcattt attacggcac gggtggtttc gtcctgtcgt
     8161 gcgctcatga gtatggcatt ttctgcagga acctcatgtc tacagcaggc ggtaggtatt
     8221 acaacggcaa ttgccgtcag tgtgagcacc accggtcgaa acattttacg tatgtttatg
     8281 ttatttctca tgaaattcct tttattgtga ccggcagggg acattcaacc cctaccggcc
     8341 tctaagatac cataaaaacc gataggatta tggtatttgg cgttactaca ttttataatc
     8401 ggcatcgtat tcatcgatcc aattgtcgac ttcggccgag aattcaatgg ctgaatcctc
     8461 aatctcaaca tcggggaggt cgcccggtac aatggtgatc tccttctcaa gttcggtctc
     8521 ggccgtgccg tcgccatccc cgtcaaccat tatcacgtcg taatcgttgg gattgccgtt
     8581 atagtcaaga ccatcgttgt catagctgtc ttctatggtc aaaatgccgt ctccgttctt
     8641 atcggcaata ccttccagtt ttccgtctcc gtctatgtcc atccacagaa tcttgatgtc
     8701 gccgactccg tcgccatcct cgtcaatcca gtattcaccc tcgttgtcgg tgatcagata
     8761 acccgaggga ttctgataat tgtcaacaaa accgttgtaa tcgatgttgg gattttcagg
     8821 ataattggga tcgtctggat cggtaacaac accgggaacg acttcaattt ctcgctccaa
     8881 ctctgtgtca acaacacaat cgccatt
//
LOCUS       DAOSGL010000056         8902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6688_length_8902_cov_4.272861, whole genome shotgun sequence.
ACCESSION   DAOSGL010000056 DAOSGL010000000
VERSION     DAOSGL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6688_length_8902_cov_4.272861"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3666
                     /locus_tag="PKY68_02960"
     CDS             <1..3666
                     /locus_tag="PKY68_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24030.1"
                     /translation="VGVKCRIDTNSLGWSTTTGANNTGIASNQQGDFTVPFWENWSPN
                     NFTGKMYQVIENVPNGDYLLKAAVFCNNGGSGLYLYANDDKVLANTTAPTYHEVMTRV
                     TNNRLEIGIVMEEATNNWVGIDNVSLTYYNFYPSFTKTATGAMVKTTKELLDALKYND
                     YAIGDIIIELQDCADESGIYMIGTGTAFPQTGGDLTVKAAEGAKPQIWGSFSSTNGMK
                     VNNITFEGLHWNGGDSTLPGFNAEAYQPFGILRADSVLGDYTVRNCTFKDLDYQRIFR
                     SNNCAGAVVKKVVFEYNDFDNMGWNRPAGSHGQTFIQMVNSNNYEMDHFVFRENLVMN
                     FHGNQLFNMPRKGTTADSAEVNFSVTIENNTFYRLGGHGNSERNFLEYNQNLGGLNGV
                     ININNNVIYDRWSGEKFPQSKLALFTPAEGQNLTVNILNNFFYPDTVVTKDPVYTPED
                     TTYYPNLRLDPVIDHNRNDLFLSDFPGLVLVPGELLSYKSPLYTAGTEGTYVGALSSY
                     YVPGFIEQPTGAIVKNLAELTEALAYTDYESADITIELMNCTDEGGVYLLGNSGFAFP
                     QTGGDLTIKNAEGQNPVLAGRLTSNNGVKAATLLYEGLEFNAQSVYDAYNTDTYSPFY
                     MTGIDSVMVGFNVRNCLFTNLPFKQLILSANKIANYAVIKDFVMEHCVIKDVGVNQDT
                     MAQHSGHMFQLSNKKTYEFDHFTFRNNIVNNWRNSQVFNINRDGTTADSTDVNFVVTI
                     ENNLFYKFGGNATNVRNFLEYTKSLGGLTGVININNNIFYERGSQAGFPHAKLTMFFP
                     APGQSVTLNVLNNFFYPDNVVKDESTPENYPNLRLVGPAETDITFSRNDLFMDSFEGL
                     SKVFRNEARLTISKQSPLFEAGTGGSFIGPEQIYVGNAGTPVIAFLTKQKTMAATAAT
                     VENDPVYLMMAADDNFIVDQIVLTDVSATSPVDLDAYDIALVQESFGGGDKILTPAGP
                     LGLAKFDLPVLYNKTYAFKAGRALASGQPGSGAEQQGLSITVDPAQQGNDLFKGLTFA
                     DDNTLTLFKAPAMDDGTLGAEKSIKALNYAKEVVLSGENTPIAIPTGVTDAVIFINDL
                     PAGTVVGGETLAERMITVGFNFGAMCADNGTNMTNAAFTIWRNALYMLAGMEVPSDPY
                     DALPQTDGVQKNVVFTMGGNLYVQATEQTNINIYNVMGALVRTVRLEQGLNTIEGLSQ
                     GQIYVVKIDSEAIKVVL"
     gene            3858..4154
                     /locus_tag="PKY68_02965"
     CDS             3858..4154
                     /locus_tag="PKY68_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24031.1"
                     /translation="MTEEQQSLLIQFEARVRQAIALCKTLREENKALKEALLQKELSF
                     LNIQEELRDISQKYDRLKLAKMMSANDDDLKATKQRLSKLVREINTCIALVNES"
     gene            4157..4453
                     /locus_tag="PKY68_02970"
     CDS             4157..4453
                     /locus_tag="PKY68_02970"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOH24032.1"
                     /translation="MKDDTFRIELYLAEKYYPLRIKRSKEEAYRKAAKLVNDAVTEYR
                     NKFGIRGSDLDIKDILAMVACHFAFEKLEFEKSSDQNILMESISKLDAELGEII"
     gene            4554..6083
                     /gene="rny"
                     /locus_tag="PKY68_02975"
     CDS             4554..6083
                     /gene="rny"
                     /locus_tag="PKY68_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOH24033.1"
                     /translation="MYIVYLVLALSVGIFLGWLIFRYILNGKYLYKIKKAELEAEAIR
                     KNKMQEIKDKFFAMKAELEKQITQRSNRVQSYENKVKQREMQLNQKQDELQRKKNELE
                     IIRENLEAQLEIIDKKKQELDKQHRKGVERLEALSGLSAEEAKERLVESLKEEAKTDA
                     ASYINEIMDEAKMTANKEAKRIVVQSIQRVATETAIENAISVFHIDSDEIKGRIIGRE
                     GRNIRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLALHQLVSDGRIHPARIEEV
                     VAKVRKQIEEEIVETGKRTTIDLGVHGLHPELVRMIGRMKYRSSYGQNLLQHARETAN
                     LCAVMASELGLNPKKARRAGLLHDIGKVPEDEPELPHALLGMKLAEKYKEKPDICNAI
                     GAHHDEIEMQTLLAPIVQVCDAISGARPGARREIVEAYIKRLNDLEQLALSYPGVIKT
                     YAIQAGRELRVIVGAEKVDDKNTEQLSAEIAKRIQDEMTYPGQVKITVIRETRAVSYA
                     K"
     gene            6089..6487
                     /gene="ssrA"
                     /locus_tag="PKY68_02980"
     tmRNA           6089..6487
                     /gene="ssrA"
                     /locus_tag="PKY68_02980"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(6594..7376)
                     /locus_tag="PKY68_02985"
     CDS             complement(6594..7376)
                     /locus_tag="PKY68_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24034.1"
                     /translation="MSLFISYKQKRGRFLPALLCSCLVFLPVKAHSSGAYLSEANNLL
                     LNTGFEIWENNLPAHWFGSASNIPETSVFQCAEAHSGESSCRLVRTQKTHVRFSSAPL
                     FLNLGNYRLSYYVKGSGCIRNSFYNGSSYAAYSDYDTLSNQQEWMKKVYEFELKKDVE
                     VQLLFSLCLTDSIGLFIDDVLFESIPAGLNRPVEFSPLIRTAQGLLQLQLPKPQSLVV
                     YDMLGRKVCERNVQGDLSIPLSRGYYLIALSGHPKPVKVYIP"
     gene            complement(7563..8549)
                     /gene="obgE"
                     /locus_tag="PKY68_02990"
                     /pseudo
     CDS             complement(7563..8549)
                     /gene="obgE"
                     /locus_tag="PKY68_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
     gene            complement(8549..>8902)
                     /locus_tag="PKY68_02995"
     CDS             complement(8549..>8902)
                     /locus_tag="PKY68_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOH24035.1"
                     /translation="KKGVIFDGFPRTIAQAEALDEILEKRNEKLNLLLHLKVADEILQ
                     ERMLFRSKTSGRSDDTPETIKKRIDIYHQVTAPVIDYYRHRGLYHAIKGNGSGTVETI
                     FAEVSSYISAYQQKR"
BASE COUNT         2613 a         2078 c         2035 g         2176 t
ORIGIN      
        1 gttggtgtca aatgtaggat tgacaccaac tccctgggct ggagcaccac cacaggagca
       61 aataataccg gcattgccag caatcagcaa ggcgacttta ccgtgccttt ctgggaaaac
      121 tggagtccga ataacttcac cggcaagatg tatcaggtaa tcgaaaatgt ccccaacgga
      181 gactacttgc tcaaagcggc agtattctgc aacaacggtg gcagcggcct gtatctctat
      241 gctaacgacg acaaggtgtt ggctaacacc actgcgccta cttaccacga agttatgacc
      301 agggttacca acaacaggct ggaaatcggt atagtaatgg aagaagccac caacaactgg
      361 gtgggtatag acaacgtaag cctgacttac tataacttct atccttcctt taccaagaca
      421 gctaccggag ctatggtaaa aaccaccaaa gaactcctcg atgctctgaa gtacaacgat
      481 tatgccatcg gcgacatcat cattgaactg caagactgtg ccgacgaaag tggtatttac
      541 atgattggaa caggtacagc tttcccgcag acaggtggag atcttaccgt taaggccgca
      601 gaaggcgcta agccgcaaat ctggggtagc ttcagctcca ccaacggcat gaaagttaat
      661 aacatcactt tcgaaggcct gcattggaac ggaggcgact ctactctgcc cggatttaac
      721 gccgaagctt atcagccctt tggtatactc agggccgact ctgttttagg cgattatacg
      781 gtcagaaatt gtaccttcaa ggatctggat taccaacgga ttttccgctc gaacaattgc
      841 gccggtgccg tcgtcaaaaa agtggtattc gaatacaatg atttcgacaa catgggctgg
      901 aacagacctg ccggaagtca cggacaaacc tttatccaaa tggtcaacag taataactac
      961 gaaatggacc actttgtatt cagggaaaac cttgtaatga atttccacgg caaccagctc
     1021 tttaatatgc ccagaaaggg cacaacggcc gatagtgcag aagtcaactt ctcggttacc
     1081 atagaaaaca acaccttcta cagattggga ggacatggta acagtgaaag aaacttcctg
     1141 gaatacaatc agaacttagg tggtttgaat ggtgtgatca acattaacaa caacgtcatt
     1201 tacgaccgct ggagtggtga aaaattcccg caaagcaaac tggctttatt taccccggcc
     1261 gaaggacaga acctgacagt aaacatactg aacaacttct tctatcccga cactgtggtt
     1321 accaaggatc cggtttacac ccccgaggat acgacttact atcccaacct gagacttgat
     1381 ccggttatcg atcacaacag gaacgacctg ttcctgagcg acttcccggg tctggtattg
     1441 gttcccggag aactgctttc ttataagtcg cctctctaca ctgccggtac cgaaggaaca
     1501 tacgttggtg cgttgagctc ctactatgtg cccggcttca ttgagcagcc caccggtgct
     1561 attgtcaaga acctggctga acttaccgaa gctttggctt acaccgatta cgaatcggca
     1621 gacatcacca tcgagttgat gaattgtacc gacgaaggcg gtgtttacct cttgggtaat
     1681 tcaggatttg ccttcccgca aacaggtggt gacctcacca tcaagaatgc tgagggacaa
     1741 aatcccgtac tggccggacg cttgacctcc aacaacggag ttaaggcagc cacactgctc
     1801 tacgaaggac ttgagtttaa cgctcagtcg gtatatgatg cctacaacac agatacttat
     1861 tcacctttct atatgactgg tatcgactct gtcatggtag gattcaatgt tcgcaattgc
     1921 ttgtttacga acttgccgtt caaacagttg attctttccg ccaacaagat tgccaattac
     1981 gcagtcataa aggacttcgt tatggagcat tgtgtgatta aggatgtggg tgtcaatcag
     2041 gatacaatgg ctcagcattc gggccatatg ttccagcttt ccaacaagaa gacctatgag
     2101 tttgaccact tcactttccg caacaacatc gttaacaact ggcgcaacag tcaggtgttc
     2161 aacatcaaca gagatggtac gacagccgac tctaccgatg taaactttgt tgttactatc
     2221 gaaaacaact tgttctacaa gtttggcgga aacgcaacca acgtcaggaa cttcttggaa
     2281 tacaccaagt cgctgggtgg tttgaccggt gtaatcaaca tcaacaacaa catcttctac
     2341 gaaagaggca gccaggccgg tttcccgcat gccaagctga ccatgttttt cccggcaccc
     2401 ggacagtcag ttaccctgaa tgtcttgaac aacttcttct atcccgataa tgttgtgaag
     2461 gatgagtcca ctcccgagaa ctatccgaac ctaagattgg tgggaccggc agaaactgat
     2521 attacattca gccgcaacga cctgttcatg gatagcttcg aaggtttgag caaagtcttc
     2581 agaaacgaag ccagattgac catctccaag cagtctccgc tgtttgaagc cggaacaggt
     2641 ggaagcttta tcggacctga acaaatctac gttggtaacg ccggcactcc cgtcattgct
     2701 ttccttacca agcaaaagac tatggctgcc acggctgcta ccgtagaaaa cgatccggtt
     2761 tatctgatga tggctgccga cgacaacttt attgttgacc aaattgttct caccgatgtt
     2821 tcggccactt cgcccgtcga tttggatgcc tacgacatcg ctctggttca ggaatccttt
     2881 ggtggcggtg ataagatcct tacccccgca ggacctctgg gattggccaa gtttgacttg
     2941 cccgtgctct ataacaaaac ctatgccttc aaggcaggta gagctctggc cagcggacaa
     3001 ccgggttcag gtgcagaaca gcaaggtctg agcattaccg ttgatcccgc acaacaaggc
     3061 aacgacctgt tcaagggatt gacgtttgct gacgacaaca ccctgacact gttcaaagct
     3121 cctgcaatgg acgatggtac attgggtgca gagaaatcta tcaaggccct caactacgcc
     3181 aaagaagtgg tgctctccgg tgagaacacc ccgatcgcta tcccgaccgg tgtaaccgac
     3241 gccgtgatct ttatcaacga cctgcctgca ggtaccgttg tgggcggaga aacactggcc
     3301 gaacggatga ttaccgttgg attcaacttc ggtgccatgt gtgccgataa cggaaccaac
     3361 atgaccaatg ctgcctttac catctggaga aatgctctct acatgctggc aggtatggaa
     3421 gttccgagcg atccttacga tgctctgcct caaacagacg gagtacaaaa gaacgtagtc
     3481 tttactatgg gaggcaacct ctatgttcag gcaacagaac aaaccaacat caacatctac
     3541 aacgtgatgg gtgctttggt aagaactgta agacttgagc agggactcaa caccatcgaa
     3601 ggactatctc agggacagat ctatgtggtt aaaatagatt cggaagccat caaggtggtt
     3661 ctctgataag accctcatat tttaagctaa ctctatagct taaatacact ataacaagcc
     3721 ccggcgaata tctcgccggg gcttgtgtgt ttataaaacc cctatgaaca gaagaccttt
     3781 acagaatata aaaaacttga attttatttg tttttggcaa aactaaagtt tacttttgta
     3841 gacaatttcg gtgggttatg acggaggaac aacaaagttt gctaatccaa ttcgaagcca
     3901 gagtacggca agccatcgca ctctgcaaaa ctctcaggga agagaacaag gcacttaaag
     3961 aagccttatt gcaaaaggaa ctgtcttttc tcaatattca ggaggagctt cgggatatct
     4021 cacagaaata cgacagactt aagttagcaa aaatgatgtc agccaatgac gatgacctca
     4081 aagctaccaa acaacgcctg tcaaaattag tgcgggagat caatacatgc attgcactcg
     4141 ttaatgaatc atagcaatga aagatgatac ttttagaatc gaattatacc ttgccgaaaa
     4201 gtattatccg cttcgaataa aaagaagcaa agaagaagct tatcgcaaag cagctaaatt
     4261 ggttaacgat gcagtgaccg aataccggaa taaatttggc atcaggggat cagacctgga
     4321 tattaaagat atcctagcta tggtagcctg ccattttgct ttcgaaaaat tagaatttga
     4381 aaaatcttct gaccaaaaca tcttgatgga aagtatttct aaactggatg cagagttggg
     4441 agaaattata taattcaact gagactacaa attccgcact acagctatca aggtgttgac
     4501 cttggtattg tagtgttttt ttattttatc taatacacac gtctatggat ataatgtata
     4561 tagtttattt agtattggct ttgtcggtgg gtatcttcct gggatggctg attttccggt
     4621 atatcctgaa tggaaaatac ctctacaaaa ttaaaaaagc cgaactggaa gcagaggcta
     4681 ttcgcaagaa taaaatgcag gaaatcaaag acaaattttt tgcaatgaaa gccgaactgg
     4741 aaaaacagat cacacaaagg agcaacagag ttcaatcgta cgaaaacaaa gtcaagcaaa
     4801 gagaaatgca gctgaatcaa aagcaagatg agctgcaacg caagaaaaac gagcttgaaa
     4861 tcatccgcga gaacctggaa gcacaattgg aaataatcga taagaaaaaa caggaactcg
     4921 ataagcaaca ccgtaaggga gttgaacgtc ttgaagcttt gtccggatta tccgccgagg
     4981 aggcaaaaga aagactggtg gaatcgctga aagaagaagc caaaacagac gccgcttcgt
     5041 atataaacga gattatggac gaggccaaaa tgacggccaa caaagaagcc aaacgtattg
     5101 ttgttcaatc gatacaacgc gttgcaacgg aaacggccat agagaatgcc atcagtgttt
     5161 ttcacatcga ttcggacgaa atcaaaggca ggatcattgg ccgggaaggt cgtaacatac
     5221 gcgctttaga agccgcaacc ggtgtggaaa tcattgtaga cgacacccca gaagccattg
     5281 tcctttcggc tttcgacccg gtgagaagag aaattgcccg cctcgcgctt catcaactgg
     5341 tttccgacgg ccgcatacac cctgcgcgca tcgaagaagt cgtagccaaa gtgcgcaaac
     5401 aaatcgaaga agaaatcgtt gaaacgggca aacgtaccac aatcgatctg ggcgttcacg
     5461 gtttacatcc ggaattagtc agaatgattg gccggatgaa atatcgttct tcgtatggac
     5521 aaaacctgct gcagcacgcc agagagaccg ctaatctctg tgccgtaatg gcttcggaac
     5581 tgggactgaa tcccaaaaaa gcccggagag caggcttgct tcacgacatc ggcaaagtac
     5641 ccgaagacga accggaattg ccgcacgcac ttcttggcat gaaacttgcg gagaaataca
     5701 aagaaaaacc cgatatttgc aacgctattg gcgcgcatca cgacgaaatt gaaatgcaaa
     5761 ccctgctggc tcctattgta caggtttgcg acgccatatc gggagcaagg ccgggagccc
     5821 gccgcgaaat tgtagaagcc tacataaaaa ggctgaacga tcttgaacaa ttggcacttt
     5881 cctaccccgg agtcatcaag acctatgcca ttcaggcagg tcgggaactc agagtaatag
     5941 tgggtgccga aaaagtggac gacaagaata ccgaacagct ttctgcagag atagccaaac
     6001 gtattcagga tgaaatgaca taccccggtc aggtgaaaat cacggttatc cgtgagacca
     6061 gagccgtgag ttatgcaaaa tagaataggg ggccgattgg ttttgacagc ggtcaaaaca
     6121 ggtaggtaag cacgcagtgc tatggtaata cgcactttaa tatccttacc aaaccaataa
     6181 ctggcgaaaa caattacgca ctcgctgctt aatcgaagta tagtagatct aagcttaatt
     6241 tctgtcacag tggcagaaac gggtcatcac cccggtgctg tggttccgaa gcggccggac
     6301 caggtggtgc agcaatatcg gaaataggca gggaaaggct ccggttcccg gccgaaattt
     6361 aaggagataa gctccgggta gatagcttcg gttttgcccc gggtcgaaaa actaaacgaa
     6421 gataagcgtg tagaaagcct attggttttc cgtttggacg agggttcgat gccctccggc
     6481 tccaccaaaa ggacatccgc caaacttgcg aaaagcagtt tggcggatgt tttacatata
     6541 ataaatgttg caccaacact ccggctattg atgcacgcca attaaaacaa aaatcaggga
     6601 atgtacacct taacgggctt tgggtgaccg gataatgcaa tcagataata accacgcgac
     6661 aaaggaatac tcaaatcgcc ctgaacattt ctttcgcaga cttttcgccc gagcatatcg
     6721 tacactacta atgactgcgg cttcggtaat tgcagttgga gcaagccctg agctgtcctg
     6781 attaaaggag aaaactcaac aggccggttg agtccggcag gaatgctttc gaataggacg
     6841 tcatcgataa acaaacctat cgaatcggtt aaacaaagcg aaaacaacaa ttgcacttct
     6901 acatctttct tgagttcaaa ttcgtagact ttcttcatcc attcctgttg attgctcagg
     6961 gtgtcgtagt cggaataggc ggcgtaggaa ctgccgttgt aaaagctgtt gcgaatacag
     7021 ccgcttcctt tcacataata acttaacctg taattaccga ggtttaaaaa taaaggagca
     7081 gagctaaagc gcacatgggt tttttgggta cgcaccaaac gacaggaaga ctcaccggaa
     7141 tgcgcttcag cacactgaaa caccgaagtt tcgggaatgt tgctggcaga accaaaccag
     7201 tgggcgggta aattgttttc ccaaatttca aaacccgtgt tcaacaacaa attgttggct
     7261 tcagagagat aagcacctga agaatgggct tttaccggta agaaaacaag acatgagcac
     7321 agcagtgccg gtaaaaaacg gccccgtttc tgcttgtaag agataaataa actcataaca
     7381 gtataatttt taagttaaac atagtaccag ataaatccgg ttaattcgcg gcaaatatac
     7441 tgtttatttt ggtttaaatt ctcttttgaa tgatatttat ttaataattt attagcatat
     7501 cgtctatgtt cccaagaatc actgaggcgg ggttgattaa tcgtttaaag gaaggttctt
     7561 tattgattca gggccgacca aagcaaatcc tttaactcgg ttaagccggt ctgatctata
     7621 gacgatatta tgactgaagg gatttcgggc aattccttgc ggatggcggc tctgagctcc
     7681 gcatcgagca tatctgcctt ggtaatggca agcaccctgg ccttatcgag caattcagga
     7741 ttgtattgct tcaattcgtt gagtaatata gtgtattctt cgatgatatt tttgctgtcg
     7801 cagggtatca taaacaagag gacagaattc ctttcgatgt gacgaagaaa acgcaaaccg
     7861 attcccttgc cttcggaggc tccctcaatt attcccggaa tatcggccat cacaaatgag
     7921 aagccgtcac ggtaagagac tatgcctaga ttgggttcca gggtggtaaa aggataatcg
     7981 gcaattttgg gctttgcggc cgacaatacc gaaagcaggg tggatttccc ggcattggga
     8041 aaaccgacca ggcccacgtc ggccaagagt tttaattcca aaatcaccgt acgttcttcc
     8101 atgggttctc cgggctgtgc ataacgaggt gcttgattga ctgccgtcct gaaattccaa
     8161 ttacccaggc ccccacggcc tcctttcaga agtacgattt cttccccgtc ttcattcact
     8221 tcacaaagaa attcctcgct gtcggcatcg taagcaactg tacccagggg aacttcaatt
     8281 atggtatcct taccggagcg tccggtgctt ctggatttgc cgcccggttc tccgtgttcg
     8341 gcaaaaatat gtctttggta acgcaaatga atcagcgtcc agtaattccg gtttccacgc
     8401 aaaataatat gccctcccct gcctccgtcc ccaccgtcgg ggccgccctt ggggatgtat
     8461 cttccgcggt ggaaatgagt tgagccggga ccgccgttac cggaacggca gtagattttt
     8521 acgtaatcaa cgaaattact ttctgccatt atctcttttg ctgataagcc gaaatgtaac
     8581 tactgacctc ggcaaatatc gtttcgacgg tgccgcttcc gtttcccttt atggcatgat
     8641 ataagcccct atgcctgtaa taatcaatga ccggagccgt aacctgatga taaatgtcta
     8701 tacgtttttt gatggtttca ggggtgtcgt ctgaacgtcc ggaagttttg cttcgaaaca
     8761 acattctctc ttgtaaaatt tcgtcggcca ctttcaggtg caacaacaga tttagctttt
     8821 cgttcctttt ttccaatatt tcatccaaag cttcagcctg agctatggtt ctgggaaatc
     8881 catcaaagat cacccctttt tt
//
LOCUS       DAOSGL010000057         8875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6711_length_8875_cov_4.408390, whole genome shotgun sequence.
ACCESSION   DAOSGL010000057 DAOSGL010000000
VERSION     DAOSGL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6711_length_8875_cov_4.408390"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PKY68_03000"
     CDS             <1..910
                     /locus_tag="PKY68_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOH24036.1"
                     /translation="PVGSAYSPALDRQNLESLGMQVAAVELTQVVRQEHQSLLLDNAT
                     ALRRALAEQKVTEIPMLSTGKQTDVLSIRGEDLTDALQSAYDRCGIDECKVICRSNKQ
                     AVRYNLGIRSRIFFKEEELSTGDLLLVAKNNYFWSQSYKEIPFIANGDLIEVRRVRKI
                     TELYGFRFADLEVRFPETDWEMEVKVILDSLVSEAPALDQAAQQKLYEAVLLDYADVR
                     SKKALYAKLRVDPWFNALQVKYGYAMTCHKAQGGQWKEIFLDQGWVTPEMLGAEYYRW
                     LYTALTRAVERIYLVNFKDEYLINRD"
     gene            923..1654
                     /locus_tag="PKY68_03005"
     CDS             923..1654
                     /locus_tag="PKY68_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOH24037.1"
                     /translation="MTGLPNYTYRILARDLDFTGRASLMSLCRYIFNTASKDAENNGF
                     GTRELHNNNATWVISRMSFEMYATLREFDEFYINTWVNEISRLMTTRNLLLCDPEGKT
                     LGAAVTQWAMIDVDSRTPMDLRENMNYSKAARFDIPCPINKPVKIIPGKESEPVLERK
                     VVYSDLDFNRHVNSVKYLEWMLDMLPLENIINNGFSRLDMNYLREALYDDRLLLSYQQ
                     EDNHHLFDIKRKDETPVCRARIQWE"
     gene            1651..2136
                     /locus_tag="PKY68_03010"
     CDS             1651..2136
                     /locus_tag="PKY68_03010"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOH24038.1"
                     /translation="MSEKFYYEAPWARLIITIEDDQLSGIEFLSAETESSLNHRKHIF
                     NKGQTAQKVCKQLDEYFSGSRRVFDLPLRLRGTGFQKEVWEVLQQIPYGEVLSYGQVA
                     KTIGRPQAQRAVGMACNRNPVPIVVPCHRVLGTNGRLTGYAGGLDIKVRLLELEKKNS
                     K"
     gene            complement(2196..2351)
                     /locus_tag="PKY68_03015"
     CDS             complement(2196..2351)
                     /locus_tag="PKY68_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOH24039.1"
                     /translation="MAQKKAFVLRIDEKMLKELEKWAADDFRSINGQIEWILNQALKK
                     QRRSKPE"
     gene            complement(2370..3317)
                     /locus_tag="PKY68_03020"
     CDS             complement(2370..3317)
                     /locus_tag="PKY68_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOH24040.1"
                     /translation="MESKEFIKDKNGASGFIHLALNIVFLVLCVVLTVNMVNNNVKEG
                     YLALFISVMCLVWILHLIGFLIVQPNKSAVLTFFGVYSGTIKKNGFFWVNPFYGKRKL
                     SLRARNLDVEPIKVNDKNGNPVMIGLVLVWKIADTYKACFEIESEAQHMAAKGQTTVT
                     EIGLKGYEEFVRIQSDAALRKIAGLYAYDHIEDKDERLTLRSGGEEINDKLEEELGER
                     LSIAGISVVEARINYLAYASEIASVMLRRQQADAIIAAREKIVEGAVSMVELALNKLN
                     EKKVVELDDEKKATMVSNLMVVLCADEAASPVINTGTLY"
     gene            complement(3554..4027)
                     /gene="trxA"
                     /locus_tag="PKY68_03025"
     CDS             complement(3554..4027)
                     /gene="trxA"
                     /locus_tag="PKY68_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH24041.1"
                     /translation="MKKYLLVLAALLPLLSCNAQTSKSSSTNNENNKMEVINLTKADF
                     LAKVANYETNPSEWKYLGDKPAIIDFYADWCGPCRAVAPVLEELAKEYKGQIYVYKID
                     TEKEQELAAVFNIRSIPSILFVPMEGKPQMAQGALPKKTFVEAINSVLLNSASKN"
     gene            complement(4129..4839)
                     /locus_tag="PKY68_03030"
     CDS             complement(4129..4839)
                     /locus_tag="PKY68_03030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOH24042.1"
                     /translation="MENEVTYTMPRIGDKAPSFKAKTTQGDINFPEDFKGKWIILFSH
                     PADFTPVCTSEFMTFGSMAADFEALNCQLVGLSIDGLYSHIAWLRTIQEKIEYKGMKN
                     IEVKFPLIEDITMNVAKTYGMVQPGESSTQAVRAVFFIDPKGVVRTILYYPLSLGRNF
                     DEIKRILIGLQTADNFNVALPADWRPGEDVIVPTAGSCGVAKDRMENAGGDMTCYDWF
                     FCTKKLPKEEVEKKLGKK"
     gene            5031..5978
                     /gene="cysK"
                     /locus_tag="PKY68_03035"
     CDS             5031..5978
                     /gene="cysK"
                     /locus_tag="PKY68_03035"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOH24043.1"
                     /translation="MSHIAKQLIDLIGNTPLLELSRYARYKGLKAELTAKLEYFNPGG
                     SVKDRVALAMIDQAASEGKLNPGGTIVEPTSGNTGIGLALVSAVKGYRLILTMPETMS
                     LERRATLKALGAEIVLTPGSGGMKAAIDKAEEIRAGIPGAITLDQFSNAANPAVHAES
                     TAREIWKDTSGRMDFFVGGVGTGGTVCGVGKGLKSFDPSIQIVAVEPQDSPVLSGGNP
                     GPHQIQGIGAGFVPKNYQATYVDRIMQVSYNEAVKTSRELARTEGLLVGISSGAAAFA
                     ATQLALQSENRRKRIVVLLPDGGERYLSTPLFDNDQLPL"
     gene            6007..6888
                     /locus_tag="PKY68_03040"
     CDS             6007..6888
                     /locus_tag="PKY68_03040"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HOH24044.1"
                     /translation="MKKLLSVLAFSVIVFTFQAKQLSLNLKAGQIYQQEQKNQMDFQL
                     KIGGNEMLIHGNFDLVVEYLIKSAQADAYEIQMSMKNITGNVGSDQFYFSLDAASSQN
                     NPLGAVLDLIINKPIVYSMSKTGKILKMPDLSSLSQDLKLSGDTTLSKFLEEFMNANS
                     GGLMQSFYPDHEVAVGDSWTITSKIPNTGVEMTNTYTWKSSKGGKNTIEMEFENFNLD
                     FSIPIRVDDGISIRVQMEAKTGGVFQLNKKTGWINASESLFEAKSKLSIAPNAQIPEG
                     AELPLNFKVVSKQSQVK"
     gene            complement(6945..8339)
                     /gene="mnmE"
                     /locus_tag="PKY68_03045"
     CDS             complement(6945..8339)
                     /gene="mnmE"
                     /locus_tag="PKY68_03045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOH24045.1"
                     /translation="MKQDTICAISTPPGSGGIAVIRLSGPEAITIADQLFVPGKNGCP
                     VREQASHSCQYGRLMEEADKVLDYVLLSVFRGPNSFTGEDTVEFYCHGSLFIQQRLIQ
                     LLIQLGCRQAGPGEFTQRAFLNGKMDLSQAEAVADLIAADSSSANQLALKQMRGGFSK
                     DLADLREKLLHFVSLIELELDFSEEDLIFASRDELKKLADQLESKLSALCESFSLGNA
                     IKNGIPVAIVGETNSGKSTLLNKLLNEDKAIVSDIHGTTRDSIEDLVSIRGLSFRFID
                     TAGLRKTKDRIESLGIERTYQKIEQASIILWLIDSTQLSEHIDWMADRIIPRSQGKKL
                     LLVFNKTDKIAGEELGIVENLFAHIPGDRIQISAKYGQNIEELKSKLIELTNLPEFDK
                     TDTIVSNIRHFEALSAGLEAIRRVREGLQQNLSGDFIAQDIRECMYHLGLITGQISND
                     EVLGQIFSRFCIGK"
     gene            complement(8345..>8875)
                     /locus_tag="PKY68_03050"
     CDS             complement(8345..>8875)
                     /locus_tag="PKY68_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HOH24046.1"
                     /translation="RIGTTGGLQEDLPVGSFLISEKSIGLDGLLNFYAGRDKVSDRGF
                     EDAFCNHTAYLKQWARPYVVSANPELLARIGQQDMLRGVTVTANGFYGPQERQLRLKL
                     ALPGFNESLRSFRYDSYRITNYEMESAGVAGLAALMGHKAMTVCLVIVNRFASDAKTN
                     YKDRMSELITLVLDRL"
BASE COUNT         2315 a         2023 c         2131 g         2406 t
ORIGIN      
        1 gccggtgggt tcggcttaca gtccggctct tgaccgacaa aacctggagt cgttgggtat
       61 gcaggtggct gctgtggagt tgactcaggt ggtcaggcag gagcaccagt ctctcttgct
      121 cgacaatgcg accgctttgc gcagggcatt ggcagaacaa aaagtaacgg aaattccgat
      181 gttgagtaca ggcaagcaaa cagatgtact cagcatccgg ggtgaagacc ttacagatgc
      241 tctgcaatcg gcttacgacc gctgcggaat agatgaatgc aaagtgattt gccgttccaa
      301 taagcaggcc gtacgatata acctgggcat acgttcgcgc atttttttca aagaagaaga
      361 actttccacc ggcgatctct tgttggtggc caaaaacaat tatttttggt cgcagtcgta
      421 caaagaaatt cctttcatcg ctaacggcga tttgatcgaa gtaaggcgtg tgcgcaagat
      481 tactgagctc tacggttttc gttttgccga tctggaagtg cgtttccccg aaaccgactg
      541 ggagatggaa gtcaaagtga tactcgatag tctggtttcg gaagctcccg ctttggatca
      601 agccgctcag caaaagcttt acgaagccgt gttgctcgat tacgccgatg tgcgcagtaa
      661 aaaggctttg tacgctaaat tgcgtgtaga tccctggttc aacgccttac aggtaaaata
      721 cggttacgct atgacttgcc ataaagctca gggcggtcag tggaaggaaa tttttctcga
      781 tcagggctgg gtaacccccg aaatgttggg agccgagtac tatcgctggt tgtatacagc
      841 tttgaccagg gctgtggaaa gaatttacct ggtcaatttc aaagacgaat atttaattaa
      901 tagggattaa aaaaatagta gcatgacagg acttcccaat tatacgtaca ggattttggc
      961 acgtgacctg gatttcaccg gtcgagccag ccttatgtcg ctgtgccgtt atatatttaa
     1021 cacagcaagc aaagatgccg aaaacaacgg tttcggaacg cgcgaattac acaataacaa
     1081 cgctacctgg gtgatttcgc gcatgtcttt cgagatgtac gccacactca gagaattcga
     1141 tgaattttat atcaatacct gggtcaacga aatcagtcgt ctgatgacta cgcgtaacct
     1201 gcttttgtgc gaccccgaag gcaaaacgct gggagccgcc gtaacgcaat gggccatgat
     1261 cgacgttgat agcaggacac ccatggattt gcgcgaaaat atgaattaca gtaaagccgc
     1321 caggttcgac ataccttgcc ccataaacaa gccggtcaaa ataattccgg gcaaagagtc
     1381 cgagccggtg ctggaacgta aagtagtgta cagcgacctg gacttcaacc gccatgtgaa
     1441 cagtgtaaag tatctggaat ggatgttgga tatgctcccc ttggagaaca tcattaataa
     1501 cggattcagc cgtctggata tgaactattt gcgcgaagct ttatacgatg acagactgtt
     1561 attgtcctat cagcaggaag acaatcatca tttgttcgac atcaaacgta aagacgaaac
     1621 acctgtttgt cgggccagaa ttcaatggga atgagcgaaa aattctatta cgaggcacct
     1681 tgggccaggc tgattattac cattgaggac gatcaactga gcggcattga gtttcttagc
     1741 gcagaaacag aaagttcact taaccatcga aagcatatat tcaacaaggg ccaaactgcg
     1801 caaaaagtct gcaaacaact ggatgagtat ttttccggaa gtcgccgggt cttcgatttg
     1861 cccctccggc tcaggggtac cggtttccaa aaagaagttt gggaagtatt gcaacaaatt
     1921 ccttacggtg aggtgcttag ttacgggcag gtggccaaga ctataggccg tccccaggcg
     1981 cagcgtgccg tgggcatggc ttgcaaccgc aaccctgttc ccattgttgt accctgtcac
     2041 cgggtgctgg gcactaacgg ccgcttgacc ggttatgccg gcggactcga cattaaggtt
     2101 cgtttgctgg agctcgaaaa gaaaaatagt aaataaggcc tcgttcagta atcattttca
     2161 cgcggtgccg ggtttcgaaa acccggcacc gctgtttatt ccggctttga gcgtctttgc
     2221 tttttcaatg cctggtttaa aatccactct atttgcccgt tgatgcttcg aaaatcgtcc
     2281 gcagcccatt tctccaattc tttgagcatc ttttcgtcga ttcggagtac aaaggctttt
     2341 ttttgcgcca taacgatcgg gttacggttt taatacaagg tgccggtatt aatcaccggg
     2401 cttgctgctt cgtcggcaca aagcaccacc atgaggttgc ttaccatagt ggctttcttt
     2461 tcgtcgtcga gttcaaccac tttcttttcg ttgagtttat tcagggccag ttctaccatg
     2521 gaaacggctc cttcgacgat cttttcgcgg gcggcaataa tggcatcggc ctgctggcgc
     2581 ctcaacatga cactggcaat ttccgaagca taagccaggt aattgatgcg ggcttccacc
     2641 acgctgatac cggcaatgga gaggcgttcg ccgagttctt cctccagttt gtcgttgatt
     2701 tcttcaccac ccgaacgcaa agtcagtctt tcgtccttgt cttcgatatg gtcgtaggca
     2761 tacaggccgg caattttacg cagagcggca tcgctttgta tacgcacaaa ttcttcgtaa
     2821 cctttcaggc caatctccgt aactgttgtt tgacctttgg cagccatatg ttgcgcttcc
     2881 gattcaatct cgaagcaggc cttgtaggtg tcggcaattt tccagaccaa aaccagcccg
     2941 atcatcaccg ggttgccgtt tttgtcgttc accttgatgg gctcaacatc caaatttcgg
     3001 gcacggagcg aaagcttcct tttgccgtaa aaagggttga cccaaaaaaa tccgttcttt
     3061 ttaatggtgc ctgaatatac accaaaaaag gtcaatacgg ccgatttgtt gggttgtaca
     3121 atcaaaaaac ctatcaggtg caaaatccac accaggcaca taacactgat aaaaagcgcc
     3181 aggtaaccct ctttaacatt gttattaacc atattgacgg tcagaacaac acacagcaca
     3241 aggaaaacga tgttcagggc cagatggata aaaccactgg caccattttt gtctttgatg
     3301 aattcttttg attccataat gatatcattt tgatattaaa cttgctgcaa atgtaattgt
     3361 attttacaat atgcaaaaac aaaaagcttc aaaattgctc ttgacggatt ttacgtccgg
     3421 gaaagagagt tttgaagctt taatccagag ccggggagca aagccttggc tttgagtatt
     3481 gcccttacgg gagtatttca gaattatttc ttctgagccg acgattcctt tcggtgttgt
     3541 gaatttaaac tatttagttt ttacttgcgc tgttgagcaa aacactgttg atggcctcca
     3601 caaaagtctt tttgggcaaa gcaccctgag ccatttgcgg cttgccttcc ataggcacga
     3661 acaagataga ggggatggag cgaatgttga atacggcagc cagttcctgc tctttttcgg
     3721 tgtcaatttt gtagacgtat atctgtcctt tgtactcttt ggccagttct tccagtacag
     3781 gtgccacagc ccggcaggga ccgcaccagt cggcgtaaaa atcgatgatg gccggcttgt
     3841 cgccgagata tttccattca ctggggttag tttcgtagtt ggctactttg gccaaaaaat
     3901 ctgcttttgt aagatttatt acttccattt tgttgttttc gttattggtt gatgaactct
     3961 tgctggtctg tgcattgcac gatagtaaag gcaaaagggc ggccaaaaca agaagatact
     4021 ttttcatatg gttggttttt aaaaaggagg gtgactgtat attcaggcac cctctcgaac
     4081 ttttaagact aaacctgtaa gactaaacct ggtgttaacc tgatgaattt acttttttcc
     4141 tagttttttt tctacttctt ctttaggtag ttttttggtg cagaagaacc agtcgtagca
     4201 ggtcatatcg ccgccggcgt tttccattcg gtcttttgct accccgcaag atccggcagt
     4261 gggcacaatc acgtcttcac cggggcgcca atcggcggga agtgctacgt tgaaattgtc
     4321 ggccgtttgc agtccgatca atatccgttt gatttcgtca aaattgcgac ctagagagag
     4381 ggggtaatat aatatagtgc gaaccacacc cttggggtcg ataaagaaga ccgctctaac
     4441 ggcttgtgtg gagctttctc ccggttgcac cattccgtag gtcttggcta cgttcatggt
     4501 gatgtcttcg attaacggga atttgacttc gatatttttc attcccttgt attcaatctt
     4561 ttcttgaatg gtgcgcagcc aggcaatatg gctgtacaaa ccatcgatcg acaatcctac
     4621 cagctggcag ttgagtgctt cgaaatcggc agccatactg ccaaaagtca taaattccga
     4681 agtgcaaacc ggtgtaaagt cggccgggtg gctaaacaat atgatccatt ttcctttgaa
     4741 atcttcgggg aagttgatgt cgccctgagt ggttttggct ttgaaagacg gagctttgtc
     4801 tccgattctc ggcatagtgt aagttacttc attttccata gttgttgaat ttattaaatt
     4861 agtaattgat ttttcgttgg tacaaagata ctaaggccgg cactgcgtac caacagataa
     4921 tatctatgag gttataggta gcgtcaattc cataaaaaaa cacaaaaaac cattgattta
     4981 taaaaaaaca ctacatttgc aactgatcta ttaggattca aaaaaatcag atgtctcata
     5041 tagctaaaca attgatcgat ctcataggca atactccctt gcttgaatta agccgctatg
     5101 cgcggtacaa aggcctcaaa gcggaattga cggccaagtt ggaatatttc aatccggggg
     5161 gtagcgtaaa ggatcgcgtc gccctggcca tgattgatca ggcagcatct gagggtaagc
     5221 ttaatcctgg cggaaccata gtggagccca ccagtggcaa taccggtata ggtttagccc
     5281 tagtttctgc tgtgaaaggt taccggctta ttctgactat gcccgaaacc atgagtctgg
     5341 aacgtagggc caccttaaaa gcactggggg ccgaaatcgt cctgactccc ggaagcggtg
     5401 gtatgaaagc tgccattgac aaggccgaag aaatcagagc cggtataccc ggagccatta
     5461 ctttggatca attcagcaat gccgctaacc cggctgtaca tgctgagagt acggcacgtg
     5521 aaatatggaa agacacttcg gggcgtatgg atttttttgt aggtggagtg ggcacgggtg
     5581 gaaccgtctg cggagtgggc aaaggtctga aatcttttga tccttccatt cagattgtag
     5641 ctgttgaacc gcaggattca ccggttttat cgggtggcaa tcccggacca caccagatac
     5701 agggtatagg agccggcttt gttcccaaaa attaccaggc aacgtatgta gaccggatta
     5761 tgcaggtaag ctataatgag gcagtcaaaa ccagccggga actggcccgt accgaaggct
     5821 tgttggtagg tatttcctcc ggtgccgcag cctttgctgc tactcaatta gctctgcaaa
     5881 gtgagaaccg gcgcaaacgt attgtggttc ttttacccga cggaggggaa cgatatcttt
     5941 ccactccctt gtttgacaac gatcaacttc ctttatgacc ttattttaac acataatctt
     6001 tttactatga aaaaactact ttcagtattg gctttcagtg ttattgtttt cacttttcag
     6061 gctaagcaac tgagcctcaa tctcaaggcc ggtcagattt accaacagga acaaaaaaat
     6121 caaatggatt ttcaactgaa gatcggagga aacgaaatgc tcatccacgg aaacttcgac
     6181 cttgttgtcg aatatttgat taaatctgcc caagccgatg cctacgaaat acaaatgagt
     6241 atgaagaata ttacgggtaa tgtcggcagc gatcaattct atttcagtct ggatgccgcc
     6301 tcctctcaaa acaatccttt gggcgccgta ttggacctta taataaataa accgattgtt
     6361 tatagcatgt ccaaaacggg caaaattcta aaaatgcccg atctgagcag tttaagtcag
     6421 gatctgaaac tgtcgggaga taccactttg tctaagtttc tggaggaatt tatgaatgca
     6481 aactccggcg gtttgatgca atccttctat cccgaccatg aagttgccgt cggcgacagc
     6541 tggaccatca ccagcaagat tcccaacacc ggagtagaaa tgaccaacac ctatacctgg
     6601 aaatcgagca agggcggtaa gaataccatc gaaatggagt tcgaaaattt taatttggat
     6661 ttcagtatac ccatcagagt cgacgatgga ataagcatac gcgttcaaat ggaagctaaa
     6721 acaggaggag tatttcagtt gaataaaaaa acaggctgga ttaacgcttc ggaatccctg
     6781 tttgaggcaa aatctaaact cagcattgcc cccaatgccc aaatacccga aggagcggag
     6841 ttgccgctga acttcaaggt agtttcaaag cagagtcagg ttaaataaaa tggtcttccg
     6901 acaggacaag actcaggttg atttaaacga taagttaaga ggttttattt tccgatgcaa
     6961 aagcgactaa agatttgtcc taatacttcg tcgttgctga tctgaccggt aataagcccc
     7021 aggtggtaca tgcattccct gatgtcctga gcgataaaat cgccggacaa gttttgttgc
     7081 aaaccttccc gaacgcgcct tatggcttcc aggccggcgg agagggcttc gaagtgcctt
     7141 atgttgctga ctatggtatc ggttttgtca aattcgggca gattggtcag ttctatcaat
     7201 ttggatttga gttcttcgat gttttgtccg tatttggccg atatttgaat gcggtcgccg
     7261 ggaatatgcg caaataagtt ttcgacaata cctaactctt cgccggcaat cttatcggtt
     7321 ttattgaaga cgagcaagag ctttttcccc tgactgcggg gaataatgcg gtcggccatc
     7381 caatcgatgt gctcgctgag ctgggtggag tctatcaacc agagaataat ggatgcctgt
     7441 tctattttct gatacgtgcg ttctatgccc aggctttcga tgcggtcttt ggttttgcgt
     7501 aagcctgccg tatcgataaa acgaaaacta aggcctctta tgctgaccag gtcttcgatg
     7561 ctgtcgcggg tggtgccgtg tatgtcggaa acaatggctt tgtcttcgtt gagtaatttg
     7621 ttgagcaaag tcgatttgcc ggagttggtt tcacccacta tggccaccgg tatgccgttt
     7681 tttatggcgt tgcccaggct gaaggattcg caaagggcag aaagtttcga ttccagttga
     7741 tcggccagct ttttgagttc gtcccggctg gcgaaaatca ggtcttcttc gctgaaatca
     7801 agttccagtt ctatcaagga aacaaaatgc agcaattttt ccctgagatc tgccagatcc
     7861 ttggagaaac ctccccgcat ctgtttcagt gccagttgat ttgccgaaga agaatctgct
     7921 gcaatcaggt cggctacggc ttctgcctgc gacaggtcca ttttcccgtt cagaaaagcc
     7981 cgttgggtaa attctcccgg cccggcttgt cggcaaccta attgaattaa gagctgaatc
     8041 agcctttgct gaataaagag agaaccgtgg caataaaact ccaccgtgtc ttcaccggta
     8101 aaagaatttg gcccccggaa tacgctcagc agaacgtaat caagtacttt atcggcctct
     8161 tccatcagcc gcccgtattg acaactgtga ctcgcctgct ccctaacagg gcagccattt
     8221 ttaccgggaa caaacaactg atcggcaatc gtaatggctt ccggtccgga gagcctgata
     8281 acagcaattc ctcccgaacc cggcggagtg gagatggcac aaatagtatc ttgtttcata
     8341 ggtcttacag gcggtcgagt accagggtta tcagttcaga catacgatct ttgtagttgg
     8401 ttttggcatc acttgcaaaa cgatttacta tgaccaggca aaccgtcatg gctttgtgtc
     8461 ccataagagc agccaggccc gctacaccgg cactttccat ttcgtaattg gtaatgcggt
     8521 aagagtcgta acggaagctg cgcaggcttt cgttgaatcc gggcagggct aatttcaatc
     8581 gaagttgtct ttcctggggg ccgtaaaaac cgttagctgt cacggttacc ccgcggagca
     8641 tatcttgttg ccctatgcgg gcaagcagtt caggattggc cgaaaccaca tagggacgag
     8701 cccattgttt caggtaagcg gtatgattgc aaaaagcatc ttcaaatccc cggtcggaaa
     8761 ccttgtcgcg gccggcataa aaattgagca ggccgtctaa accgatggat ttttcggaaa
     8821 tcaggaagct gcccaccggc aagtcttcct gcaggcctcc ggtggtgcct atcct
//
LOCUS       DAOSGL010000058         8787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6807_length_8787_cov_4.650481, whole genome shotgun sequence.
ACCESSION   DAOSGL010000058 DAOSGL010000000
VERSION     DAOSGL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6807_length_8787_cov_4.650481"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKY68_03055"
     CDS             <1..230
                     /locus_tag="PKY68_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24047.1"
                     /translation="AQPPDIHGWSALDAGVACLSLRACTGKGCRPLRASGAVSIYRLR
                     GWPFAAVRFRCGQIRYCTSPFSPSLSGMFIE"
     gene            682..1044
                     /locus_tag="PKY68_03060"
     CDS             682..1044
                     /locus_tag="PKY68_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24048.1"
                     /translation="MKTNKLKNLLLFLVVLCNLYVVSGKTQGEKAAQAVQRWDKEVSL
                     SETQKNQVEALALQFIQQLDSINALPLDFFERQLMKKETHTRHLSDILSLLTEQQRKQ
                     FDRATAERKKLIDNKKKK"
     gene            1056..2759
                     /locus_tag="PKY68_03065"
     CDS             1056..2759
                     /locus_tag="PKY68_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24049.1"
                     /translation="MKTNLIKHLIAGVLFFGLHLQLSAATYNITNTSGEIDVEYYNYI
                     NNIQDVYIFNTNTFSTISFYLIINSEEDYDYVEIWDVDNSGNETLLYEFSGEYSGIVT
                     TTSPSGRAKVYIRTDHSYCYYDGDYDLTGFYIGYSAVGNYTSTQSSINENNLSIVGKL
                     GVGIRTPQEKLHVDGAIRGNVGNTGALRVKTTQGYIDIGPSTPNYANFRTNQSRFLFD
                     RSLFIENGTLSAYNTSNLSLQTNGTARMTILSANGNVGIGYAIPMEKLHINGSIRGNG
                     SKGALRVKTMQGYIDIGPSITNYANFNTNQSRFLFDRPLFIGNGELSAYDTSNLSLQT
                     NGTTRMTILSASGNVGIGIPTPQEKLHINGPIRGSASSTGALRVQTTGGYIDIGPQNT
                     YDAHFSTDKGSFVFNQTIYSGTGVFGTLNDKNLVFKTNSNSTRMTILQSNGNVGIGTT
                     SPDYKLDVAGVIRCSEVLVQGIDQIADFVFHPDYSLPSLQQVDKFISSNGHLPGIPSE
                     AEVKEKGLGLVEMQIKLLQKVEELTLYVIEQEKRALEQEKRVLEQERRIKELEAELMN
                     K"
     gene            2771..5680
                     /locus_tag="PKY68_03070"
     CDS             2771..5680
                     /locus_tag="PKY68_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HOH24050.1"
                     /translation="MKRYIFWSVCVKQTVSSLSILLLLSVVCQQTQAQDDFGTVTPQT
                     SDFMEYGETPVSLFNGRINLDIPIYTIKDRDFTIPISLTYTSDGFKPKKRSGFVGHDW
                     ILNAGGCITREVYGEADDLFIDEPNVGYGRLLGYWWAIEDPNYQFDPENVWNHDGSLI
                     SYLLLGVDYRQDLFLFNFCGHKGQFMIDNNRELQSNIKSYRVNFSGFNKQYMSDIIPR
                     ESTITITTPDGYRYEFGGSRGALEFTLRYYPGTTVSNEIAHPTILAWHLKKITAPNGR
                     TVKFNYVEIDMTQSNVNSFSPIWQSNKISKTSSLHSAQKTVILESLELDGTLVEFVRS
                     TEQNADQFCNTGYSEFNGNCFQLDAIRVKQNNQLVYEYTFQYETRNKRRFLSSVELPD
                     ENKYLFYYNHSSAYPVPETMSEDLYGYWKNNDAQNSYGLINKVIYPTGGYTTFVYEKN
                     FYGKRVETRTSGNLVHPLYGEVEVFLTTESAETGGARIRKITHYSQPGHEASSKEYFY
                     QPPGYPLLGNAIHYDTTIIVRPDFFDESCLCWRRVPDSIVISVDSSLSTLSSSGILYK
                     YPPYVLSPNGSRSFIENIWFQNYNIGEPHIGYSHVVELNSDSSMTAYTFTDYSKCPDY
                     YDNNISAPFPADRLLAFNIDRSSSSAAKRGHLIKKDTYDADKLKIRSEEYFYDHINAS
                     HPKPSQDSIIYYDRGIITLLLTGNTSHTDIIIISKGIKLLHFPLVCYKDSINGVVRKE
                     IYQYNSEDLLHKKSLALHAGDSLKTFYKYPSDFAALGNTYDQMVNRNIVNIPVEEQTY
                     RNNNLLQTTRYVHKLHNGSPVVDTLKVGYNNNPPEPRIVYHNYDQYGNPVYTTENNDI
                     HKVFLWSYMGKYLVAEIQNASYNDLTTVMPNLLALNPNPSASDLSAVNALRSSLENAL
                     VTTYTYKPLVGVTSITDPRGVKSTYEYDPFNRLKCIKDHFGNTIKQFEYHYYSEE"
     gene            5711..6754
                     /locus_tag="PKY68_03075"
     CDS             5711..6754
                     /locus_tag="PKY68_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH24051.1"
                     /translation="MKQKYILPPLIIACGFFSALSSIRYDLNHFRAGDHYTRQEMEYV
                     QPGDTGINVLWNFQQLDVINEKNPVYTFLKTREDTCRFVVMEHLTRFHYLYREDTLWL
                     TGFENKTVKMDFFGHEARLRFPFHYGDSLCSPFYGTGIYCQKIPLVAKGRTSVKADAT
                     GTLITPDNDTLHHVIRVKQQRSYTETGHDSVNLKMETYSWYARGYRYPIFETVTITER
                     HIDSTEVGFKNSFYYPLPDMELIPEDILNEQLRQEDVPADSTEQVILNCVTFPNPVRN
                     TLGIRYYLSKEVSVSYRICDMNGFPVIFLSEGRLSQGDHQHIISMSQCLPGDYILYIL
                     VDQKVFTKTIIKQ"
     gene            6760..>8787
                     /locus_tag="PKY68_03080"
     CDS             6760..>8787
                     /locus_tag="PKY68_03080"
                     /inference="COORDINATES: protein motif:HMM:NF039454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6443 domain-containing protein"
                     /protein_id="HOH24052.1"
                     /translation="MNTKINIILLCCLLILSVKGKAQIGTIVLDSVMPPVTMLVVATE
                     SVVLKPGFHYKGNQGEFRAKIDNYNALTPLVNLGEGSTVNTELSGENKNYIVTKTSLS
                     SGNNPPTLNTVNYFDGLGRKEEQVQMGISPLGADMISLIEYDQASREQKQWLPAAIAG
                     GFGQFIASPELQNHAANFYDDAYCYTETLYEPSALNRVATQWGPGAAWRTANRATHIE
                     YLSNNSSNPVVHFKVNTNKDLVREGFYDPSKLYLTKTTNEENQEVYEYKDKQGLLLLS
                     RLVANATNYDTYYVYDQSDLLCYVLSPEASSRITGDGTYSSQGINTIGNALDQYAFQY
                     RYDNRNRCTKKKLPGSDWIYMIYDQCDRLVLSQDGSQRQLPYPQSNWSYIKYDPLGRV
                     IESGVTLLSQSFNALQQYYKNISVVETYNTETSGYSCNHSFGLNDTALIRYYYDGYQL
                     PSSFPAQLVYQETNGALSSFDARYDVGPSPAAGLLTGSEVSMLKDPNKKLYSVIYYDY
                     KARPVQQLSTNHTGGIDYDFFAYNFTDQVLRQRHVHTAFEEDADYSSGPFYEDYHFTY
                     DHAGRQLSCRYTLSNNDSILSSVNLNTMTYDELGRMQQKTIHQSVLSGSFTYNIRGWL
                     TSIASPVFNEQIYYQEAQSGQTPYYNGNISSISWGFCGRTDKAYSFSYDQLN"
BASE COUNT         2748 a         1831 c         1771 g         2437 t
ORIGIN      
        1 ccgctcagcc tcccgatatc cacggctggt cagcgctgga cgccggcgtt gcctgtttgt
       61 ctctgcgagc ctgcacaggc aaaggatgcc ggcctctgcg cgcttcgggc gccgtgtcta
      121 tatatcggct gcggggctgg cctttcgctg cggtacgctt tcgttgtggt cagattcggt
      181 attgcacctc tccttttagc ccaagtctgt ctggtatgtt tatagagtag agcaaagcat
      241 tgagaaattt gtccattttc gggggtctta aaatactctt ttcaaaaaaa cgcggtttta
      301 tcgtgctttt ttagaattgc cgatgaaatg acactttttt gcactaaact gtgctaaaaa
      361 gaaacgattc tgttcaaaaa gagcgaaact tttaatacct atccagcaga ttttcaaaca
      421 catacagcga aacttttcga gcaaccaaaa aactcttatg ttttttgttg cacctttaga
      481 aagcaaaaaa tcttgtttaa gattttattt gctgcaaaca aattagatga ataacacaaa
      541 cccaaaatta acatcgtaac taacaccata ataaagacac tcaaatgaaa aagcctttgt
      601 ttctatttat cttcttttgt ttactcggtg atatcagtgt gaaatttaaa gtcattaaac
      661 aataaacaaa acaccacgaa catgaaaacc aacaaattaa agaacctact ccttttttta
      721 gtcgtgttat gcaatttata cgttgtatcc ggaaaaacac aaggagaaaa ggccgcacaa
      781 gctgtccaaa gatgggataa ggaggtatct ctgtctgaaa cacaaaaaaa ccaggttgaa
      841 gccttagcac tacagtttat acagcaatta gatagtataa atgctctgcc actggatttt
      901 ttcgaaagac aattgatgaa aaaagagaca cacacaaggc acctgtccga cattttaagt
      961 ctgctgacgg aacaacaaag aaaacaattc gacagggcta cggcagaaag gaaaaagctt
     1021 attgataaca agaaaaagaa ataaaaacag aagccatgaa aacaaattta ataaaacatc
     1081 ttattgcggg agtcttgttt tttggcctgc acttgcaact gtctgcagcc acatacaaca
     1141 taacgaacac tagtggagaa attgacgtcg aatattataa ttatatcaat aatattcagg
     1201 atgtatatat tttcaacaca aacacatttt caacaatttc tttttatttg attatcaact
     1261 cggaagaaga ttatgattat gttgaaatct gggatgtaga taattccgga aatgaaactt
     1321 tgttatacga atttagcggt gaatactcag gtatagtaac aaccacatcg cccagcggca
     1381 gagctaaagt ttatattaga acagaccatt cgtattgtta ttacgatggt gactatgatt
     1441 tgaccggttt ttatattgga tattctgcag ttggtaacta cactagcaca caaagtagta
     1501 taaatgagaa caatctttct attgtgggga aattgggcgt tggaatcaga actccacagg
     1561 aaaaactgca tgttgatggc gccataaggg ggaatgtggg aaatacggga gctttaagag
     1621 tgaaaactac gcagggttat attgatattg gtcccagtac acctaattac gctaatttta
     1681 gaaccaatca atctcgattt ttattcgata gatccctttt tattgaaaat ggaacactta
     1741 gtgcgtataa tacaagcaat ctgtctttac aaacgaatgg caccgcccga atgaccattt
     1801 tatctgctaa cggaaatgtt ggaatcggat acgcgattcc tatggaaaag ttgcatatta
     1861 atggttctat aagggggaat ggaagtaaag gagccttaag agtgaaaacc atgcaaggtt
     1921 acattgatat tggccccagc attactaatt acgccaattt taacaccaat caatctcgtt
     1981 ttttgttcga cagacccctc tttatcggta atggagaact tagtgcgtat gatacaagca
     2041 acttatcttt acaaacgaat ggcactactc gaatgactat tttatctgct agcggtaatg
     2101 ttggaatcgg catcccgact ccccaagaaa aactacatat aaacggtcca attcgcggaa
     2161 gcgcaagtag taccggggca ttaagggtgc aaactacagg gggttatata gacatcggcc
     2221 cacaaaacac ttacgatgcc catttttcaa ccgataaagg atcctttgtt tttaaccaga
     2281 cgatctattc gggtacagga gtttttggca ctttgaacga caaaaattta gttttcaaaa
     2341 ccaactccaa ctcaacacga atgacgattc tgcagagcaa tggcaatgtg ggcattggga
     2401 caacatctcc ggattataaa ttagatgtgg ccggtgttat ccgttgttct gaagtgttgg
     2461 tacagggtat tgatcaaatt gccgactttg tttttcatcc cgactactct ttgcccagtc
     2521 tgcagcaagt ggataagttt atatcgtcca acgggcattt gccgggcatt ccttccgagg
     2581 ccgaagtaaa agaaaagggc ttaggattgg tggaaatgca gataaaatta ttacagaaag
     2641 tggaagaact aaccctgtat gttattgagc aggaaaagcg agctcttgaa caagagaaaa
     2701 gagtccttga acaagagaga agaatcaaag aactggaagc cgaattaatg aacaaataat
     2761 tccccgcctt atgaaacgtt atatattttg gagtgtttgt gtaaaacaaa ccgtttcatc
     2821 attgtctatc ttgttgttgt tgagtgttgt atgtcaacaa acacaggcgc aagatgattt
     2881 tggtaccgta acaccacaga cctctgattt tatggaatat ggagaaacgc cggtctcact
     2941 gttcaatgga agaatcaacc ttgatattcc tatttatacc atcaaggacc gcgattttac
     3001 tatcccgatt tctttaactt atacttctga tggttttaag ccaaagaaaa gatccggatt
     3061 tgtgggtcac gactggattc tgaatgcagg aggatgtatt acacgcgagg tgtatggaga
     3121 agccgatgat ctctttatag atgaaccaaa tgttggatac ggtcgtttgc taggctattg
     3181 gtgggcaatt gaagatccta attatcaatt tgatcctgag aatgtttgga atcatgatgg
     3241 cagccttata tcttatttgc ttttgggggt tgattacagg caggatttgt ttctttttaa
     3301 tttttgcggt cataaggggc agtttatgat tgataataat cgtgaactac aatccaatat
     3361 aaagtcttat agggtgaatt tctccggatt caataaacaa tatatgtccg atatcattcc
     3421 gagagaatcg acaattacca ttaccacccc ggacgggtat cgatatgaat tcggaggatc
     3481 acgtggagct ttggaattta ccttgcgcta ctatccggga acgactgtct ctaacgaaat
     3541 tgcccatccc accatcttag cctggcatct aaaaaagatt actgcgccaa atggccggac
     3601 tgttaagttt aattatgtgg aaatagatat gactcagtcc aatgtgaatt cattttcacc
     3661 tatttggcaa tcaaataaaa tatcaaaaac cagctcttta cactctgcac aaaaaacagt
     3721 gatattagag tccctggaac tggatggaac tttagtagag tttgttcgtt ccacagagca
     3781 aaatgccgat caattttgta ataccggtta ttcagaattt aatggaaatt gttttcagtt
     3841 ggatgccatc cgagttaaac aaaacaatca attggtttat gaatacactt tccaatatga
     3901 aacacgcaat aaaagacgct ttttaagctc tgtggaattg cccgacgaga ataaatatct
     3961 gttttattat aatcattcat ctgcttatcc tgttccggaa acaatgagtg aagatttgta
     4021 cggatattgg aaaaacaatg atgcacaaaa ttcctatgga ttaatcaata aagttatcta
     4081 tcccaccggc ggttatacga cctttgtcta cgaaaaaaat ttttatggta aaagggtgga
     4141 aacgcgaact tcgggaaatc ttgtacaccc tttgtatggg gaagtcgagg tctttctgac
     4201 tacagagagt gcagagacgg gtggggcaag gatccgaaaa atcacacatt attctcagcc
     4261 cggccatgaa gcgtcgagta aggaatattt ctatcaacca ccggggtatc ctttgttggg
     4321 aaatgcgatt cattacgata ccacaatcat agttcgcccg gatttttttg atgagtcatg
     4381 tctttgttgg agaagagttc ccgacagtat cgttatatcc gttgattcca gcctatcgac
     4441 tttatccagc agtggcatct tatataaata tcccccatac gtactctctc ctaatgggag
     4501 ccgtagtttc attgaaaata tctggtttca aaactataat attggcgagc cacacattgg
     4561 ttactctcac gtggttgagt tgaattcgga cagttccatg acggcctata cttttaccga
     4621 ttattcgaaa tgtccggatt attacgacaa taatatttca gcccctttcc cagccgatcg
     4681 cctattggct tttaacatcg acagaagctc cagttcagct gccaaaagag gtcatttgat
     4741 aaagaaagat acctatgatg ccgataaatt gaaaatcaga tcggaagaat atttttacga
     4801 tcatattaat gcatcccatc caaaaccaag tcaagattcg ataatttatt acgatagagg
     4861 tatcattacc ctacttctta ccggaaatac ttcgcataca gatatcataa taattagtaa
     4921 aggaattaaa ctgctacatt ttccgttggt ttgctacaaa gattccataa atggtgttgt
     4981 aagaaaagaa atctatcaat acaatagtga agatttattg cataaaaaat ccctggcgct
     5041 tcatgcagga gattccttaa aaaccttcta caaatatccc tcagattttg ccgccctcgg
     5101 aaatacttac gatcaaatgg taaatcgtaa catagtaaat atccctgttg aagaacaaac
     5161 ataccgtaat aacaacttgc ttcagaccac aagatatgtt cataaactcc acaacggatc
     5221 gccagtggtc gataccctaa aagtcggtta caataataac cctcccgaac cacgcattgt
     5281 gtatcacaat tatgatcaat acggcaatcc tgtctatacc acagaaaaca acgacataca
     5341 caaagtgttc ctatggagct acatgggcaa atatcttgtg gctgagattc aaaatgccag
     5401 ctataacgat ctgacaactg tgatgccaaa cctgctggct ttaaatccca acccatcggc
     5461 ttccgacttg tcggccgtta atgcactgcg ctcttcgctc gaaaatgccc tggtaacaac
     5521 ctatacttac aaacctctgg ttggagtcac atccattaca gatccccggg gtgtgaaaag
     5581 cacttacgaa tacgatccgt ttaatcgctt gaaatgcatc aaggatcatt ttgggaatac
     5641 gattaaacaa tttgaatatc actactattc cgaagaatag ttgaagccga aacaccaaaa
     5701 tcaatctttt atgaaacaaa agtacattct acccccatta ataatagcct gcggtttctt
     5761 ttctgccttg agtagtattc gttacgattt gaatcatttc agggcaggag atcactatac
     5821 ccggcaggaa atggaatatg ttcaaccggg agataccggg ataaacgttc tttggaactt
     5881 tcaacagttg gatgttatca acgaaaaaaa tcctgtttac acctttttga aaaccagaga
     5941 agatacctgt cgatttgtag tgatggagca tctcacacgt tttcactatt tatataggga
     6001 agataccttg tggttaacag gctttgaaaa taagaccgta aaaatggact ttttcggtca
     6061 cgaagcccgc ttaagatttc cttttcacta tggagatagt ctttgctctc ctttttacgg
     6121 tacggggata tactgccaaa aaataccttt agtcgccaaa ggcagaacca gcgtaaaagc
     6181 cgatgccaca gggacattga taacccccga caacgacacc ctgcatcatg taataagagt
     6241 aaaacagcaa cgctcttata cagagacagg ccacgacagc gtgaatctta aaatggaaac
     6301 ctatagttgg tatgcgcggg gatatcgtta tcccattttc gagacggtca ctataaccga
     6361 acgccatatt gacagtacag aagtgggatt taaaaactcg ttttattatc ctttgccgga
     6421 tatggaatta attcccgaag acatccttaa cgaacaactg agacaagaag atgtgccggc
     6481 agacagcaca gagcaagtta tcttgaattg cgtcaccttt cccaatcctg tcagaaacac
     6541 actcggcata cgttattacc tatcaaaaga agtctcagtt tcgtatcgta tctgcgatat
     6601 gaacggcttc ccggttattt tcctttccga aggaaggcta tcccagggag atcatcaaca
     6661 cataatttca atgagccaat gtctccccgg ggattacatt ctgtatattc ttgttgatca
     6721 aaaagttttt acaaaaacga tcataaaaca ataagccaga tgaacacaaa gataaatata
     6781 atactacttt gctgtctttt aattttgtcg gttaaaggca aggcccaaat aggcacgatt
     6841 gttctcgaca gcgtcatgcc accggtaaca atgttggtgg ttgccaccga aagtgttgtc
     6901 ttaaagcccg ggtttcatta caaaggaaat cagggagagt tccgtgccaa aatagataat
     6961 tataacgccc tgaccccttt ggtcaacctg ggggaaggaa gcaccgtcaa cacagaatta
     7021 agtggagaga acaagaatta cattgtcaca aaaacaagct tgtcgtccgg aaacaatccc
     7081 ccaaccttaa acacggttaa ttattttgat ggactgggca gaaaagagga gcaagtacaa
     7141 atgggaataa gtcctctggg ggccgatatg atctctttaa ttgaatatga ccaagcaagt
     7201 cgtgagcaaa agcaatggct gcctgcggca atagcgggag gttttgggca gttcatagca
     7261 tcgccggagc tacaaaacca tgctgccaat ttctacgacg atgcctattg ttacacagaa
     7321 accctgtatg agccttcggc tctcaacaga gttgccactc aatgggggcc cggagcagct
     7381 tggagaacag caaacagagc gacacatata gagtatctga gcaataattc cagtaatcct
     7441 gttgtgcatt tcaaggtcaa caccaacaag gatttggtca gagagggttt ttatgatccg
     7501 tccaaactgt atctaaccaa aacaacgaac gaagaaaatc aagaggttta cgaatacaaa
     7561 gacaaacaag gtctacttct tctcagcagg cttgttgcaa atgccaccaa ctacgatact
     7621 tattatgtct acgatcaatc ggacctactc tgttatgtcc tttctcccga ggcctcttca
     7681 agaataaccg gtgacggcac gtacagttct cagggtatca acaccattgg aaatgctttg
     7741 gatcaatatg cctttcagta ccgatacgac aaccgtaaca gatgcacaaa aaagaaattg
     7801 cccggatcag actggattta tatgatatat gaccaatgtg accgtctggt gctcagtcaa
     7861 gacggaagtc agcggcagtt gccttacccg caaagcaatt ggtcgtatat aaaatacgac
     7921 cccctgggaa gagtgatcga atcaggtgtg accctactgt ctcagagttt taatgcctta
     7981 cagcaatatt acaagaatat ttccgtggtc gagacctaca ataccgaaac ctccggctat
     8041 tcttgcaatc attcatttgg cctcaacgat acggcattga tccggtatta ttacgacggc
     8101 tatcaattgc catcgtcctt ccccgcgcag ttggtgtatc aagaaacaaa cggagcgctg
     8161 tcctcttttg atgcccggta tgatgtaggg cccagtcctg ccgcgggatt gttgaccggc
     8221 agcgaagtgt ctatgctgaa agatcctaac aagaagctgt attcggtcat ctattacgac
     8281 tataaagctc gtcccgtcca acagttgtcg accaatcata ccggaggcat cgattacgat
     8341 ttttttgctt ataatttcac ggatcaggta ttgcgtcaac gccatgtaca taccgccttt
     8401 gaagaggatg ccgactatag ctccggccct ttttacgaag attaccattt cacttacgat
     8461 cacgccggac gccaattgtc ttgccgctat accctgagta ataacgacag tatattgtcc
     8521 tcggtaaact taaacaccat gacctacgat gaattggggc ggatgcaaca aaagacgata
     8581 catcagagtg ttctgtcggg tagtttcacc tacaacattc gaggctggct gacgtccatt
     8641 gcctctccgg tgtttaatga acagatttac tatcaggagg cacaaagcgg tcaaactcct
     8701 tattataacg gaaatatttc atccatcagt tggggtttct gcggccgcac agacaaagca
     8761 tacagcttct cgtacgatca gctcaac
//
LOCUS       DAOSGL010000059         8740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_6856_length_8740_cov_4.078181, whole genome shotgun sequence.
ACCESSION   DAOSGL010000059 DAOSGL010000000
VERSION     DAOSGL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6856_length_8740_cov_4.078181"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKY68_03085"
     CDS             <1..362
                     /locus_tag="PKY68_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOH24053.1"
                     /translation="TYQTDDLLEPDVPHFDEDKLLEKIDHDTDLMKSLLEMAAEEYPK
                     FLQQMKTACENKDACSVKSIAHTLKGSAYNLEMTVLGDIARKMEFHHEDPEVLLLLLE
                     SLENEWKNVQNILEQKQ"
     gene            461..1555
                     /locus_tag="PKY68_03090"
     CDS             461..1555
                     /locus_tag="PKY68_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOH24054.1"
                     /translation="MSHDKKHLIYVIDDNPMNLLLTSKILESYGFETQTAESGAAALQ
                     LIRQQRPSLILLDINMPNMDGYEVCRRIKAKEDWADISVIFLTANTQTENLVEGFNVG
                     GVDYITKPFRREELWVRVSTHLELAESRRTIIEMNRSRDRLYSIIAHDIRSPLSGILQ
                     TLEAIDKGYIGLNGDELREIIKLLGERTKETSTLLNSLLQWTRIQTDSQQLEFKATDL
                     FVLIQSCVDLLRPNAEQKELSIEVEVEEELRAWCDELSIHTVVRNLLSNAIKFTPRGG
                     RVRIGAGKKDGQAMIYVADNGVGMDSETVERIFAKDQHFTSSGTENEQGTGLGLMLVK
                     DFVKKNNGSLQVDSEPGEGTEFRVYLPLNN"
     gene            1563..2930
                     /locus_tag="PKY68_03095"
     CDS             1563..2930
                     /locus_tag="PKY68_03095"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOH24055.1"
                     /translation="METSGRNILIIDDDPVYRRLSSSILKERFTVFTADAPSAGFEIL
                     KNEKIDYVICDYRLPEMDGLGVLERIKTDYPHIEVIMVSDSGNMDTVIEALRRGAVDY
                     FKKPFTPADIWMSIEKTQRFTKLKKDFQLEKNKSSRLRQWVDKEVSIQMIGESPAIEE
                     IKNQVRLVARTPDTSVLIIGESGTGKELVARGIHDLSARRDELFGAVNMSAIPESLFE
                     SEFFGHKKGSFTGAIADKAGWFETTDKGSLFLDEIGDMPHNLQVKLLRVLEEKKYIKV
                     GTQKEQTFDIRIIAATNKSLEEISNPKFFRTDLFHRIGTFIIHLPPLRKRKEDIPLLV
                     EYFVEQFSEKMAKSNIQVAPEVFDLLQGYSFPGNIRELRNLIERAVILCKDNIIKAEN
                     FGIINKLAHSAPQEEDSYDLQEVEKRAISKALDISGDDQEEAAKLLNIDSKTLSKKMK
                     QHGLL"
     gene            2945..4351
                     /locus_tag="PKY68_03100"
     CDS             2945..4351
                     /locus_tag="PKY68_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOH24056.1"
                     /translation="MKKNSPTLLAILIIITTVVLLLMYRSPNNIYRPDEKYRAILRAV
                     KAPVLPEKTLSILDFGALGDSVTDCRPAFEKAMQACRELGGARIVVPRGKYFLDGPIH
                     LVSNVCLDLREGAELFFGSNPRSYLPVVRSSWEGTFLYNYSPFIYGYQLKNISIIGKG
                     SIDGRAAEGFAEWADLDDDDQALSREMNYAGTPVEERIFGEGHFLRPQLIQLFECKNV
                     LIEDVMLKDSPFWCLHLLKSENITVRGIRFSAFNKNNDGIDPEYSRNVLIENVQFNNS
                     DDNVAIKAGRDYEGRKTNTPSENIIIRHCRFKGLHAVVIGSEMSSGVRNVFVHDCDHG
                     GYLKRGIYLKSNPDRGGYIRDVYINNLNLGTVEDCFFITSNYHNQGKGFETDIRDIHV
                     NNLHCKEAENAGIVIQGFETKKVRDIYLSNVNIEKAAIGLFMEHAENIVLDNVISGGR
                     VGAPSTAKKTDLQRIEQE"
     gene            4404..5393
                     /locus_tag="PKY68_03105"
     CDS             4404..5393
                     /locus_tag="PKY68_03105"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24057.1"
                     /translation="MIIESRAYARAGLLGNPSDGFFGKTISIIIRNFGVGVLLYESPE
                     LVIEEDEDDRFRNLRHLTETIKKTGYYGGKRLVKAAVKKFSEYCDQEGIRLPARNFTI
                     RFSTTIPRQVGLSGSSAIITATFKALMKFYEVEIPQEMLPSWVLKAESEELAITAGLQ
                     DRVIQVYEGCVYMDFDREFMLKHGRGRYEVIDPALLPKFYVAYKTRLSKVSGAVHNPI
                     REKFEAGDPLVCDTMAQIAEVAEQGRTAILNKDYDTLDKLINLNFDLRRKIYKISPAH
                     LELVEAARSCGVSAKFAGSGGTIIGMYRDEESLHRLFFKMKSLNARVIKPYIM"
     gene            5415..6293
                     /gene="galU"
                     /locus_tag="PKY68_03110"
     CDS             5415..6293
                     /gene="galU"
                     /locus_tag="PKY68_03110"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOH24058.1"
                     /translation="MIRKAVIPAAGLGTRFLPATKAQPKEMLPIIDIPTIQYVVQECV
                     ESGIEDILIITGKGKRSIEDHFDRNLELEERLLKEREDEYWYNELRHIDSMANIHFVR
                     QKEQNGLGDAIYYARFHCGSEAFAVLLGDSVNDSVIPITQQMIDTYEQYRHTVIAVQQ
                     VDKNRVDRYGIVGGTFLSDRIMQVSEMVEKPAPDKAPSNWAVAGRYILTPEIFQAIEQ
                     TPRGKGNEIQLTDALRILLQTESIIAMKIEGRRYDIGNKLDYLKTTVDFALKRKEFAN
                     DFAAYLRKKLNEFDEV"
     gene            6353..7972
                     /locus_tag="PKY68_03115"
     CDS             6353..7972
                     /locus_tag="PKY68_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOH24059.1"
                     /translation="MKTFISLLITSLLSLNLLHAQYISKVWKGDLGDGRYQNPIINAD
                     YSDPDVCLGADGYYMVASSFNCVPGLPVLFSRDLVNWSLVGHALSRQVPEDFYNKVQH
                     GSGVWAPSIRYHNKEYYVYWGNPDFGIYMVKASNPRGPWTEPHLVKAGKGLIDVCPLW
                     DEDGKAYMSFALAGSRAGLKSVLLVAPMSPDGKSLIGEPRIIYDGHEKNPTIEGTKFH
                     KHEGKYYIFAPAGGVSTGWQVVLRADQPYGPWQVRTVMHQGKTKINGPHQGAWVRTPQ
                     NEDWFIHFQDVGVMGRVTHLNPMQWKNGWPVIGLDGDNDGIGEPVSIYRKPAVPVRLP
                     VATPAESDEFDKATLGLQWQWQANPDFRWYYLDPTSGGNLRLYSFYTPGAISLWDVPN
                     LLLQKFPAPNFKATAKVRFEPDKRYKGERGGLVVMGMDYAVLCVENTEDTLLLTQNSC
                     NKADNNGTERIVAAVPLAQQDIYLRVEVKEGQYCAFHYSLDGQKYIRIGTEFKAREGK
                     WIGAKVGLFCTRPQESNDGGWLDVSYFRIEP"
     gene            7972..>8740
                     /locus_tag="PKY68_03120"
     CDS             7972..>8740
                     /locus_tag="PKY68_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH24060.1"
                     /translation="MKKHVLCCLLALLSFALPAAGITISGYISDEETGETLINATVYD
                     SISGKGAVSNLYGFYTLTLPEGPVHLIYSYLGYKPQRFAFWADSDRQLTVRLQSGAEL
                     EEVVVSGRQHKEFGVLGTQMSAVEIPVAQIKNVPSLLGENDVIKALQLLPGVQSGSEG
                     SAGLYVRGGGPDENLLLLDGVPVYNVNHMFGFFSVFNADAIKNVTLYKGNFPARFGGR
                     LSSVVDIRMNDGNDKSYHGNVSVGLISSKFNFEGPIVR"
BASE COUNT         2473 a         2008 c         2148 g         2111 t
ORIGIN      
        1 caacttacca aacagatgac ttgcttgaac cggacgttcc tcatttcgac gaagataagt
       61 tgctggaaaa gatcgaccac gataccgacc tgatgaaaag tttgttggaa atggctgccg
      121 aagaataccc caaattcttg cagcaaatga aaacggcctg tgaaaacaaa gacgcctgta
      181 gtgttaaatc catagctcat acgctcaaag gcagtgctta caatttggaa atgacagtcc
      241 tgggagatat cgcccgaaaa atggaattcc accacgaaga ccctgaggtt ttgctcctgt
      301 tgctcgaaag tcttgaaaac gaatggaaga acgtgcaaaa cattttggag cagaaacaat
      361 aattatttaa cataaacaaa atacaataaa gccattttaa tatgttatat ttgcgaccta
      421 tgggatagtt tgtacttttc ctgcctaaaa gtagatcact atgtcgcacg ataaaaaaca
      481 ccttatatac gtcatcgatg acaatccgat gaacttattg ctaacttcca agattcttga
      541 aagttacggc tttgaaaccc agactgccga aagcggtgcc gcggcgctgc aactgatcag
      601 gcaacaaaga ccttcactca ttctgctgga tatcaatatg cccaatatgg acggttacga
      661 ggtgtgtcgc cggattaaag ccaaagagga ctgggcggat atttctgtta tttttctgac
      721 agccaacaca caaaccgaaa atttggtgga aggattcaac gtcggaggag tagattacat
      781 aacaaaacct tttcgcagag aagagctatg ggtgcgggta agtactcatc tcgaattggc
      841 ggaatccaga cgaaccatta tagaaatgaa ccgttcaaga gatcgtctgt attccattat
      901 cgctcacgac atacgttctc ccttgtcggg catactgcaa actctcgaag ctatagataa
      961 aggttatatc gggctgaatg gcgatgaatt aagagaaata ataaagctgc taggagaacg
     1021 caccaaagaa accagcacct tgttgaacag tctgctgcaa tggactcgca tacaaaccga
     1081 cagtcagcag cttgagttca aagcgaccga tttgtttgtc cttattcaaa gttgcgttga
     1141 tttgttgcgt cctaacgccg aacaaaaaga attgagcata gaagtagaag tggaagaaga
     1201 actcagagcc tggtgcgatg agctttccat acacacagtg gtgcgaaacc tgctttccaa
     1261 cgccattaaa tttacccctc gtggaggaag ggtaaggatt ggagcaggca aaaaagacgg
     1321 acaggctatg atttatgttg cagacaacgg cgtaggaatg gactccgaaa ccgtggagcg
     1381 catttttgca aaagaccaac actttacaag tagcggtacc gaaaacgagc agggtaccgg
     1441 tttgggtctg atgctggtca aggattttgt aaaaaagaac aacggtagtc tccaagtgga
     1501 cagtgagccc ggggagggca ctgaattcag ggtgtacctt ccattgaata attagctgaa
     1561 ttatggaaac atcaggcaga aatattttaa ttatcgacga tgaccctgtt tacagaagat
     1621 tatcgtcttc catccttaag gaacgtttta cggtgtttac cgcagatgcc ccttcggcag
     1681 gattcgaaat actcaaaaac gaaaagatcg attatgtgat ttgtgactat cggcttccgg
     1741 aaatggacgg cctgggtgtt ttagaaagga taaaaaccga ttatcctcat atcgaggtaa
     1801 tcatggtgag cgattccggc aatatggata cggttatcga agccttgcgc cgtggggcgg
     1861 tcgattattt caaaaaacct tttacaccgg cggatatctg gatgtctatc gaaaaaacgc
     1921 agcgttttac caaactgaaa aaagattttc agttggaaaa aaacaaaagc agccgcctca
     1981 ggcaatgggt cgacaaagag gtgagcatac agatgatagg ggaatctccc gccattgaag
     2041 aaatcaagaa ccaagtgcgc ctggtagccc gcacgcccga cacttcggtg ttgataatcg
     2101 gagaaagcgg cacaggtaaa gagttggtgg ccagaggtat tcacgatttg agtgctcgaa
     2161 gggacgaatt gttcggagcg gtcaatatgt cggccattcc cgaaagtctg ttcgaaagtg
     2221 aatttttcgg acataaaaag ggaagtttta cgggcgccat tgccgacaaa gccggctggt
     2281 tcgaaaccac cgacaaaggc agcctgtttc tcgatgaaat cggcgatatg ccgcataacc
     2341 tgcaagtgaa gcttttgcgg gtgctcgaag agaagaaata catcaaggtg ggaacccaaa
     2401 aggaacagac ttttgatatc cgcattattg cagcaaccaa caaaagtctc gaagaaatat
     2461 ccaatcccaa attcttcaga accgatcttt ttcaccgtat cggcacgttt atcattcatc
     2521 tcccgcccct gcgcaaacgc aaagaagaca tacccttgtt ggtggaatac tttgtggaac
     2581 agttttcgga aaaaatggcc aaatccaaca tacaggtagc gcccgaagtc tttgacctgc
     2641 ttcaaggcta tagttttccc ggaaacatac gcgaactgcg aaatctgata gaacgggcag
     2701 tgatcttgtg caaagacaac attatcaaag ccgaaaattt cggtattata aacaagttgg
     2761 cccacagtgc tcctcaggag gaggacagct atgatttaca ggaagtcgaa aaaagagcca
     2821 tcagtaaagc attggacatc agcggtgacg atcaggaaga agccgccaaa ttgctgaata
     2881 tcgatagcaa gaccctctcc aaaaagatga agcaacacgg tcttttgtaa aaccgattca
     2941 gaagatgaaa aaaaacagcc cgaccctttt agcgatttta attatcataa caacagtcgt
     3001 gttgttgttg atgtaccgca gccccaacaa tatttaccgt cccgacgaaa aatacagagc
     3061 catattacgt gcggtcaaag cgcctgtcct tccagaaaaa accctgtcga tacttgactt
     3121 cggagctttg ggcgattctg ttaccgactg caggcccgct ttcgaaaaag ccatgcaagc
     3181 ttgtcgggaa ttgggcggag cccggatagt ggtaccgcgg ggtaagtatt ttttggatgg
     3241 acccatacat ctggttagca atgtttgtct ggatttgagg gaaggagccg aactcttttt
     3301 cggttccaat ccccgcagtt acctgccggt ggtacgcagc agttgggaag gtacttttct
     3361 gtacaattac agccctttta tttatggcta tcaactaaaa aacatttcca tcataggtaa
     3421 aggcagcata gacggaaggg cagccgaagg ttttgccgaa tgggccgatc tggacgacga
     3481 cgatcaggct ctgagccgcg agatgaatta cgccggcacg cccgtcgaag agcggatttt
     3541 tggcgaagga cattttctgc gtcctcagtt gattcaactt tttgaatgta aaaacgtgct
     3601 gatcgaagac gttatgctca aagattcccc cttttggtgt ttgcatttgc ttaaatccga
     3661 gaacatcacc gtcaggggaa ttcgcttttc ggctttcaac aaaaacaacg acggcatcga
     3721 tccggaatat tcccgcaatg tgctgataga aaacgtgcag tttaataatt ccgacgacaa
     3781 cgtggccata aaggccggcc gcgattatga agggcgcaaa accaacacac cctccgaaaa
     3841 cattattatc cgccattgcc gtttcaaagg cctgcacgca gttgtgatag gcagcgagat
     3901 gtcttcgggt gtcagaaacg ttttcgtaca cgattgcgat cacggaggtt acctgaaaag
     3961 gggtatttac ctcaaatcca atcccgaccg cgggggctat atcagagacg tatatatcaa
     4021 caacctgaat ctgggtacag tggaagactg cttttttatt acttctaatt accataatca
     4081 gggcaagggc tttgaaaccg acatacgcga catacacgtc aacaatctcc actgcaagga
     4141 ggccgaaaat gccggtatag tgattcaagg cttcgaaact aaaaaagtcc gtgatattta
     4201 tttgagcaac gtcaatatcg agaaggctgc cataggcctg tttatggaac atgccgagaa
     4261 catcgtgttg gacaatgtta tttcaggagg cagggtaggc gctccttcta cggccaagaa
     4321 aacggatttg caacgaattg agcaagaata atgctttttt gagcaaataa gctatctttg
     4381 ttgaccaatc taagctgatc atcatgatta tcgagagtag agcctatgcg cgtgccggcc
     4441 tgttgggaaa tccttcggac ggttttttcg gtaaaaccat atccatcatt atacgtaatt
     4501 tcggagtcgg cgtgttgttg tatgaatcgc cggagctggt gatcgaagaa gacgaagatg
     4561 accgttttcg caacttgcgg catctgacgg aaaccatcaa aaaaacaggc tattacggtg
     4621 gtaaacgttt agtcaaggcc gctgtgaaga aattcagtga atactgcgat caggaaggca
     4681 tccggctgcc cgcccgcaat tttactatac gtttcagcac cactataccc cgtcaggtag
     4741 gtttgtcggg ttccagtgcc atcatcacgg ctactttcaa ggccctgatg aaattttacg
     4801 aagtggagat tcctcaggaa atgctgccta gctgggtact taaggcagag tctgaagaat
     4861 tggctattac ggcaggttta caggacagag ttattcaggt ttacgaaggc tgtgtataca
     4921 tggattttga tcgtgaattt atgctcaagc atggtcgtgg ccgttacgaa gtgatcgatc
     4981 cggctttgct tcccaagttt tatgtggcct acaaaacccg tttgagcaag gtatcgggcg
     5041 ccgtacacaa ccccatcaga gaaaagttcg aagccggcga tcctttggtt tgcgatacca
     5101 tggcccaaat tgccgaagtg gctgagcaag gccgcactgc tattctaaac aaagattacg
     5161 ataccctcga caaactgatc aatttgaatt ttgatttacg ccgcaaaatc tacaagatca
     5221 gccccgctca tttagaattg gtcgaagcag cccgctcctg tggagtttcg gctaaatttg
     5281 ccgggtcggg cggaaccatc ataggcatgt accgcgacga agaatccctg catcgtctct
     5341 tttttaaaat gaagagcttg aatgccaggg taatcaaacc ttatattatg taacaaaagc
     5401 ataacacgaa aacgatgatt cgaaaagcag tcatcccggc agcagggctg ggaacacgat
     5461 ttttacctgc caccaaggcg cagcccaaag agatgctgcc tattattgat attcccacca
     5521 tacaatacgt ggtacaggaa tgcgtagaat cgggcataga agacattctg ataatcacgg
     5581 gtaaaggcaa acgttccatc gaagaccatt tcgaccgcaa tctcgagctc gaagagcgct
     5641 tgctcaaaga gcgcgaagac gaatattggt acaacgagct caggcatatc gacagcatgg
     5701 ccaacatcca ttttgtacgt cagaaagaac aaaacggcct gggcgatgcc atttattatg
     5761 cccgttttca ttgcggcagc gaggcttttg ccgttttgtt gggcgacagc gtcaacgatt
     5821 cggttattcc catcacccag caaatgatcg acacctacga gcaatatcgt catacggtaa
     5881 ttgccgtgca gcaggtggat aaaaataggg tggaccgtta cggcattgtg ggtggaactt
     5941 ttttgagcga ccgcattatg caggtttccg aaatggtcga aaaacccgct cccgacaagg
     6001 cgccttccaa ctgggccgtg gccggacgtt atattctgac tcccgaaatt tttcaggcca
     6061 tagaacaaac gcctcgcggt aagggcaacg aaattcagct taccgatgcc ctgcgtatct
     6121 tgctgcaaac agagagcatt attgccatga aaatagaagg acgtcgttac gacataggca
     6181 ataagctcga ctacctgaaa accaccgtag attttgctct taaacgcaag gaattcgcca
     6241 acgattttgc ggcctatctc aggaaaaaac tcaatgaatt cgatgaggtt tgagctccgg
     6301 caaaaaccaa aaacggacta atctcactaa cttaaacacg ttttattgaa ttatgaaaac
     6361 cttcatcagc cttttaatta catctctatt atcgctcaat cttttacatg cccaatacat
     6421 ttccaaggta tggaaaggcg atttgggcga cgggcgctat caaaatccca tcataaatgc
     6481 cgattattcc gatcccgatg tctgcctggg tgccgacgga tactatatgg tggcatccag
     6541 ttttaattgt gttcccggtt tacctgtgtt gttttccaga gacttagtca actggagttt
     6601 ggtaggccat gccttgagca ggcaagtgcc cgaagatttt tacaacaagg ttcaacacgg
     6661 cagcggagtt tgggctcctt ccatacgcta tcacaacaag gaatattacg tatactgggg
     6721 caatcccgat ttcggtattt acatggttaa ggcttccaat ccccgaggcc cctggacgga
     6781 gccgcatttg gtcaaagccg gtaaaggtct catcgacgtt tgtccccttt gggacgaaga
     6841 cggcaaagcc tatatgtctt ttgcgctggc cggtagccgg gccgggctta agtcggtgtt
     6901 gctcgtagct cccatgagcc ccgacggcaa aagtctgatc ggtgaaccgc gcataatcta
     6961 cgacggacac gaaaagaatc ccaccataga agggactaag tttcacaaac acgagggtaa
     7021 gtactatatc tttgccccag ccggcggagt ctctaccggt tggcaggtgg tgctcagggc
     7081 cgatcagccc tatggcccct ggcaggtgcg cacggtgatg caccagggaa aaaccaagat
     7141 caacggcccg catcaaggcg cctgggtcag aaccccgcag aacgaagact ggtttattca
     7201 ttttcaggac gttggcgtta tgggaagggt aactcatctc aatcccatgc aatggaaaaa
     7261 cggctggccg gtcatcggcc tcgacggtga caacgacggc ataggtgaac cggtaagcat
     7321 ctatcgcaaa ccggccgtgc ccgtacggct gcctgttgca acgccggcag agagcgacga
     7381 attcgacaaa gccaccctgg gattgcagtg gcagtggcag gcaaaccccg atttcagatg
     7441 gtattatctg gaccccacca gcggtggcaa tttgcgcttg tattcttttt acaccccggg
     7501 ggccatcagt ttgtgggatg tgcccaattt attgcttcaa aaatttcccg cccccaattt
     7561 taaagctacc gccaaagttc gttttgagcc cgacaaacgc tataagggcg aacgcggagg
     7621 cctggtggtg atgggcatgg actacgcagt gctttgtgtc gagaacaccg aggataccct
     7681 gttacttacc caaaactctt gcaacaaagc cgacaacaac ggcacggaga gaatagtggc
     7741 tgccgtgcct ttggctcaac aagatatcta cctcagggtg gaggtaaaag aaggtcaata
     7801 ttgtgctttc cattacagcc tcgacggcca gaaatatata cgcatcggca ccgaattcaa
     7861 agcccgcgaa ggcaaatgga taggggccaa agtcggtttg ttttgtaccc gcccgcagga
     7921 atcgaacgac ggcggatggt tggatgtttc gtatttcagg attgaaccct aatgaaaaaa
     7981 cacgtactgt gttgcctgct ggccttgctt agcttcgccc ttcctgcagc aggcataacc
     8041 attagcggat atatttccga cgaggagaca ggtgaaacac ttataaacgc caccgtttac
     8101 gacagtatct cgggcaaagg agctgtaagt aatctctatg gtttctatac gcttacgttg
     8161 cccgaagggc cggtgcatct tatctattct tatctgggtt ataaacctca gcgctttgcc
     8221 ttctgggccg atagcgaccg ccaactgacg gtacgtctgc aatccggtgc cgaattggaa
     8281 gaagtcgtgg taagcggaag gcaacataaa gaattcgggg tattgggcac tcagatgagc
     8341 gcggtggaga ttccggtggc tcaaattaag aatgttcctt cgttgttggg cgaaaacgac
     8401 gtcatcaagg ccttgcagct cttgcccggt gtgcaatcgg gttccgaggg ttctgccggg
     8461 ctttatgtta ggggaggcgg gccggacgaa aacctcttat tgctcgacgg agtgcctgtg
     8521 tacaatgtga atcacatgtt tggctttttt tcggtgttca acgccgatgc cattaaaaac
     8581 gtgaccctgt acaaaggcaa tttcccggcc cgtttcggag gcaggttgtc gtcggtggtg
     8641 gatatccgta tgaacgacgg caatgacaag agctaccacg gcaatgtaag cgtcgggttg
     8701 atctcttcca aattcaattt cgaaggcccc atcgtacgtg
//
LOCUS       DAOSGL010000060         8557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7063_length_8557_cov_4.109974, whole genome shotgun sequence.
ACCESSION   DAOSGL010000060 DAOSGL010000000
VERSION     DAOSGL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7063_length_8557_cov_4.109974"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..654
                     /gene="rpiB"
                     /locus_tag="PKY68_03125"
     CDS             208..654
                     /gene="rpiB"
                     /locus_tag="PKY68_03125"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOH24061.1"
                     /translation="MNPDDDVPVAICSDHAGFELKEFLCDWLTEKQIPFINFGTYSPD
                     SCDYSDFAHPLAESLEEGECKVGIAICGSGQGMTITVNKHTGIRGALCWNPEIARLAR
                     QHNNANICCLPARFISREEAVQILEAFMNTGFDGGRHQIRIDKIPI"
     gene            complement(742..1416)
                     /locus_tag="PKY68_03130"
     CDS             complement(742..1416)
                     /locus_tag="PKY68_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOH24062.1"
                     /translation="MSIASNIQEINRTLTPESKLIAVSKLQSPESIMQAYEAGQRRFG
                     ESRAQEMTAKHKVLPDDIEWHFIGHLQRNKVKYIIPFVSLIQSVDSPALLEEINKSAE
                     KTSRLVPCLLQVHIAREKSKFGFTPDELKQFMQEGSWKKLAHVRLHGLMGMATFTDNM
                     DQVRQEFRLLRHLFEEIRSGYFSKDENFKELSMGMTGDYQIALQEGSTMVRIGTALFG
                     VREKGE"
     gene            complement(1435..2715)
                     /locus_tag="PKY68_03135"
     CDS             complement(1435..2715)
                     /locus_tag="PKY68_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOH24063.1"
                     /translation="MRLHKKTFKVDLFDRIRTNEESLSKMARQLHGYFTYPKLEGPLG
                     AHMKFRGKDILNWNVNDYLGFTQNEEIRRIDEELTRKWGLSYPSGNRIMAGHTSIHEK
                     LESMLADFTHKEDAFVLNYGYQGISSVIDALCDRKDIIVYDVQVHASMIDGVRLHRGE
                     HFSYQHNDMDSLEKALQKAESKLEKDPQGGILLLTQGVFSSTGDYGKLDQIVALKERY
                     SFRIFLNDADGFGVQGKNGSGTAEHFGVSSQIDLYLGSFSKAFASQGCFIAGPELIMN
                     YLRYRTRTQLHSKTLPLVFTEGIIERLKYLKKNLSLLKKLHSNADYLRNALLEKGFDL
                     GESNTNIIPIYFPCNIYEALNIVIDLRENYNIFCPVLIYPFILKDQLLLRLMPTVYHS
                     MEDMDFTLNAFVDIRENLKAQKYSTERSDFFKDS"
     gene            2888..4303
                     /locus_tag="PKY68_03140"
     CDS             2888..4303
                     /locus_tag="PKY68_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24064.1"
                     /translation="MKTKITYLFILALAFPWAVGAQNVYDDDLYYHPKFESALIKAGS
                     TQDQPSLPANKVTENEIVLAVNNNESSIDVDVYNRRPSYKNNNPADNYKEKDLVEDSV
                     LISGEEYEDFKYCERIRRFHNDKFTTHITEDGYVNIYIEEGADVDIYYMDDYYWNPWM
                     SPWYFDNFYRPYYYGWGYHGYYGYYSPWRYSGWYYGFYDPWYYSWYSPWYSSWGWDYY
                     YYGYHPAYYYYSPYTVWGYPSYIKTPRKPVNTRTALASRNELNSRGGIPVSSRQAVGS
                     RGAAGRADSRSSAVYNDFSRAETTTARATSVRGDNLTRGTVSPGRTTATARSAGTRST
                     VNSARSSDNNASYRSSTPDNRYSGTGVRQSTTRSSSANSSRSNAVSTTPSSRSGVTTT
                     SPSNSRGSSSSVRSSTSSSVRSGTSSSSTRSSVSTSSSSSSSYSTPSSSGSSSYSSGR
                     SSSSVSSGSAVRSSSGSSGRR"
     gene            4316..5773
                     /locus_tag="PKY68_03145"
     CDS             4316..5773
                     /locus_tag="PKY68_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24065.1"
                     /translation="MKKTILAVFLFSAFGLMGQEAFDALRYSQSEINGTARSIGMANA
                     FGALGGDLTALGINPAGIAVYRSSEISFSPILSIAGLNSDFKGSVSSDSKVGFGANNF
                     GVVGSYRTYDNSKVANYNFGVTYNRLKNFTRNVSVIGLNRPVSLLDNLSPGSTLENMA
                     WETYLLDDNYNPVLQEGELLDNEQYAIEKGSIGEWNFSMGFNYNYLLYFGASLGVQSV
                     NYEIDTYYKEFFDEGGSMELWNWLKTSGTGINGKIGLIFRPIPELRLGFSYHTPTYYS
                     LRDDYDACLSSYGMPDENNIVEDRSIWISETEPDPVYYQLETPQLFVISAAAQLGQVG
                     LLSVDYERIDYRNIRMRNSSGYPFADVNEDINKFFRLGYKLRAGTEIRLDKHFCFRAG
                     YAFYQSPVANHIEKGNTVIYTPSTAPQYFMEKDAHTASLGFGYRTGVFFFDLAFVEQF
                     RTEHFYPYYNWNGAGEYANIKTNTANIVCSLGVKF"
     gene            complement(5770..7782)
                     /gene="uvrB"
                     /locus_tag="PKY68_03150"
     CDS             complement(5770..7782)
                     /gene="uvrB"
                     /locus_tag="PKY68_03150"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOH24066.1"
                     /translation="MFKLVSTYQPTGDQPEAIQLLTEGIQQGLSHQTLLGVTGSGKTF
                     TIANVVEQAQRPTLVLSHNKTLAAQLYSEFKGFFPENAVEYFVSYYDYYQPEAYLPHS
                     DLYIEKDLAINDEIEKLRLSATSSLLSGRRDVLVVSSVSCLFGIGNPVDFHNNIQRVH
                     KGQALSRNVFLRNLVDSLYSRNDVGPGRGNFRVKGDNVDIFLAYSDMVVRVCFWGEEI
                     EAIEQIDSLTGRRLAEFDRFDIYPANLFVTTKERQAQAIRQIELDLAAQVAFFQSIDK
                     AYEAKRLQERVSYDIEMIKELGHCSGIENYSRYFDGREPGMRPFCLLDFFPDDFLMVI
                     DESHVTVPQIRAMWGGDHARKQNLVEYGFRLPAALDNRPLRFDEFEAMTSQVIYVSAT
                     PADYELEKSGGLIVEQLIRPTGLLDPVMVVRPSKHQVDDLMEEIRQRAENNERTLVTT
                     LTKRMAEELSAYLKQQGVLCEYIHSDVETLERVNIMDKLRKGQFDVLIGVNLLREGLD
                     LPEVSLVAILDADKEGFLRNHRSLTQTAGRAARHINGKVILYADKITESMQLTLEETQ
                     RRRKKQEEYNKLNNITPTAIQKDNYSVLVSRAAEDSNLKKAYIEPEEINILTDPVVRY
                     MSRPQLEKLIENTRKLMYTASEKMEFLEAAQYRDELYRLEQRLKEL"
     gene            7983..>8557
                     /locus_tag="PKY68_03155"
     CDS             7983..>8557
                     /locus_tag="PKY68_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOH24067.1"
                     /translation="MIWNETYECLSRKEMRELQGKRLFNTVERVYYNSPFYRKKMQEL
                     GITPEDIESVEDLAKLPFTVKNDLRDQYPFGLFSSPMSEIVRLHASSGTTGKPIVGGY
                     TRKDLNIWSEVMARTFSAGNCDRGDMFQIAYGYGLFTGGLGAHAGAERIGASVIPISS
                     GNTEKQIQLMTDFGTTALCCTPSYALYLAEM"
BASE COUNT         2313 a         1883 c         1926 g         2435 t
ORIGIN      
        1 gcatattctt ttaatctagg gtcaataaga cacaaagata tcaggttttt tttgcttagg
       61 atttaaagtt tttatcggca agcaaattaa tcggcccggc cttgtttaaa taaattaatt
      121 tgtatctttg cctggcagaa tcaatactat gattttgagg tctcccatct gtttaaacat
      181 gagattattc catatactaa aataaaaatg aatcccgacg acgacgtgcc ggttgccatt
      241 tgcagtgatc atgccggctt tgaattgaaa gaatttcttt gtgactggct cacagaaaaa
      301 caaatcccct ttataaattt tggaacatac tcacccgaca gttgtgatta cagcgatttt
      361 gcacatcctc tggctgaatc ccttgaagag ggcgaatgta aagtgggaat agccatctgc
      421 ggcagtggac agggtatgac catcaccgta aataaacaca ccggtatcag gggcgcttta
      481 tgctggaatc ccgaaatagc ccgcctagcc cgtcaacaca acaacgctaa catctgttgt
      541 ttgccggcaa gattcatcag ccgggaagag gcggtgcaaa tacttgaagc atttatgaac
      601 accggcttcg acgggggaag gcatcagatc cggatcgata aaatccccat ttgaacgaga
      661 atgtgtcgca ctcaccggca gcactttggt tatgatccgg atcgacaaaa ttcccatttg
      721 aacccgttgg gatcaacaaa tttattctcc tttttctcgt accccgaata gggcagtacc
      781 gatgcgaacc atggtgcttc cttcttgtag ggcgatttgg taatcgcctg tcattcccat
      841 ggacaattct ttgaaatttt catccttgct gaaatatccg gagcggattt cctcaaataa
      901 atgcctgagc aagcggaatt cctgcctgac ctgatccata ttatcggtaa aagtggccat
      961 acccatcagg ccatgcagcc gtacatgagc taactttttc caactgcctt cttgcatgaa
     1021 ttgcttaagt tcgtcgggag taaaaccgaa tttacttttt tcgcgggcaa tgtggacttg
     1081 caaaagacaa ggaacaagac gagatgtttt ttctgccgat ttattgattt cctccaataa
     1141 agccgggctg tctacacttt gaattaatga aacgaatgga ataatgtatt tgactttgtt
     1201 tcgctgcaga tgacctataa aatgccattc gatatcgtca ggtaatactt tatgcttcgc
     1261 agtcatttcc tgagccctgc tttcgccaaa acgcctttgg ccggcttcgt aagcctgcat
     1321 aatgctctct ggggactgta acttggaaac cgcaatcaat ttagattccg gagtcaatgt
     1381 tctgttgatt tcctgaatat tcgatgcaat cgacatacta taaattattt gagcctacga
     1441 atccttaaaa aagtccgagc gttcagtgga atatttttgg gctttcagat tttcccgaat
     1501 gtctacaaaa gcattcagcg taaaatccat gtcttccatg gaatgataaa ctgtaggcat
     1561 aagcctaagc agaagttgat ctttcagaat gaagggataa atgaggacag gacagaaaat
     1621 gttgtagttt tcccttaaat cgattacaat gttcagggct tcgtaaatat tacaaggaaa
     1681 atagatgggt ataatattgg tattggattc tcccaaatca aaaccttttt ccagcagtgc
     1741 gttcctcagg taatcggcat tggagtgcag tttttttaat agcgataaat tctttttgag
     1801 gtacttgagc cgttcgatga tgccttctgt aaaaaccaga ggtagggttt tggaatgcaa
     1861 ttgcgttctg gtgcgatacc tcaaataatt cattattaat tccggtcctg caataaaaca
     1921 tccctgagaa gcaaaagctt tggaaaaaga gcccaggtat aagtcaattt gtgagctgac
     1981 accaaaatgc tcggccgttc cggagccatt tttgccttga acaccgaaac catcggcatc
     2041 gttgagaaaa attctgaaag aataccgttc tttcaaggct actatctggt cgagtttgcc
     2101 gtaatcgccg gtagaactga aaactccctg cgtaaggagc aagatgccgc cttggggatc
     2161 tttttcgagt ttgctttcgg ctttttggag ggctttttcc agactgtcca tatcgttgtg
     2221 ctgatagctg aaatgttccc ccctgtgcag acggacgccg tcgatcatgg aagcatgtac
     2281 ctgaacatca taaacaatga tatccttgcg atcgcacaaa gcatcaataa cggaggagat
     2341 tccctgataa ccgtaattca ggacaaaagc atcttcctta tgcgtaaaat cggcaagcat
     2401 ggattccagc ttttcgtgaa tactggtatg tccggccatg atccggttgc ccgaaggata
     2461 ggagagtccc catttccggg tcagctcttc atctattcta cggatctcct cgttttgtgt
     2521 aaaacccagg taatcattta cattccaatt cagtatatct ttgcccctga acttcatatg
     2581 agccccgaga ggtccttcca gtttaggata ggtaaagtat ccgtgcagct gtctggccat
     2641 ttttgacaaa gactcttcgt tggtacgtat gcgatcgaat aaatccacct tgaatgtttt
     2701 cttgtgcagg cgcaaaacta attaaataat tcatacctta caaaaatttt caatcctgcc
     2761 tttaatcact gtcagaggct gagattattg ttaaatctgt ttaatttaaa taaacgatct
     2821 ggcgaaaaaa gtatctttgt attaagcatg tttatcctgt tttcaccaaa aagaagggag
     2881 tactgatatg aaaaccaaaa tcacttacct atttatactt gccttagctt ttccttgggc
     2941 agttggtgca caaaatgtgt acgatgacga cctgtattac catcctaaat ttgagtctgc
     3001 tcttattaag gccggaagca ctcaggatca gccttctctt ccggcaaaca aagtaacgga
     3061 aaatgagatt gttcttgctg taaataataa tgaatcatcc atcgatgttg acgtgtacaa
     3121 ccgcaggccc tcgtataaaa acaacaatcc ggcggataat tacaaagaaa aggacttagt
     3181 ggaagattct gtgctgatca gtggagaaga atacgaagat tttaagtact gcgaaagaat
     3241 aagacgcttc cacaacgata aattcacaac ccacattacc gaagacggtt atgtgaatat
     3301 ttatattgaa gaaggcgccg atgtggatat ttattatatg gacgactatt actggaatcc
     3361 ctggatgagc ccttggtatt ttgacaactt ttacaggccc tattattacg gttggggtta
     3421 ccatggttac tatggttatt attcaccttg gcgttatagc gggtggtatt acggctttta
     3481 cgatccctgg tattacagct ggtacagtcc ctggtacagc tcttggggat gggattacta
     3541 ttattacggc tatcatcccg cttactatta ctactccccg tacacagtct ggggatatcc
     3601 atcctatatt aagacgccca gaaaaccggt aaatacccgt acagctttag cttcgcgtaa
     3661 tgagcttaat tcaaggggag gaattccggt tagcagcagg caggcggtcg gcagcagagg
     3721 tgcagcaggc agggctgaca gccgctcttc ggctgtgtac aacgacttta gccgtgccga
     3781 aaccactact gcaagagcaa ccagcgtccg tggcgacaat ctgaccagag gtaccgttag
     3841 tcccggaaga acaactgcca cagcccggtc agccggcact cgttcgacag ttaattctgc
     3901 cagatcttcc gataataatg cttcttatag aagctctact cccgacaacc gttattctgg
     3961 taccggtgtg agacaatcaa caaccagaag ttcttctgcg aacagcagcc ggagcaatgc
     4021 agttagtaca actccttctt ccagatcggg tgtcacaacg acaagtcctt caaattcaag
     4081 aggttcgagc agtagcgtca gaagcagcac gagcagcagc gtaagaagtg gcacgagcag
     4141 cagtagtaca agaagcagcg tatctacatc aagctcatca tcctcttctt attcaactcc
     4201 cagcagcagt ggcagcagtt cttattcttc cggcaggagt tcttcgagcg tcagctccgg
     4261 ttcagccgta cgcagctcat cgggcagttc cggtcgtcgc taaacacttt acgaaatgaa
     4321 aaaaacaatt cttgcagttt ttctgttttc agcattcggc ttaatgggcc aggaagcttt
     4381 tgatgcatta aggtattcac aatctgagat taacgggaca gctcgttcca ttggtatggc
     4441 caatgctttt ggtgccctgg gaggtgattt gactgccctt ggtatcaatc cggcggggat
     4501 agccgtctat cgaagttcag agatcagttt cagccccatt ttgagcatag ccggtttgaa
     4561 ttcggatttc aaaggttccg tgagcagtga ttccaaagtg ggttttggcg ctaataactt
     4621 tggtgtggta ggctcgtacc ggacttacga taactccaaa gtggccaatt acaacttcgg
     4681 cgttacttac aacaggttga aaaactttac tcgcaatgtg agtgtgatcg gcctgaaccg
     4741 ccccgtttca ttgctggata accttagccc cggcagcacc ctggaaaaca tggcctggga
     4801 aacctatctg ttggacgata attacaatcc cgttttgcaa gagggcgaat tattggacaa
     4861 tgagcaatac gcaatcgaaa aagggagtat aggcgaatgg aatttcagca tgggattcaa
     4921 ttacaactat ttactgtatt ttggggcttc cctgggtgta caatcggtca attatgaaat
     4981 agatacttac tataaggagt tctttgacga aggcggcagt atggagttgt ggaattggtt
     5041 aaaaacctca ggaacaggta ttaacggaaa aattggcctg atctttcgcc ccatacccga
     5101 attaaggctg ggcttttctt accacacacc cacttactac tcgctcagag acgattacga
     5161 tgcctgcctg agttcttatg gtatgcctga cgagaacaac atcgtagaag acaggagcat
     5221 ctggatttcc gaaacggagc ccgatcctgt ttattatcaa ttggaaactc cgcaactttt
     5281 cgttatcagt gctgctgctc aactggggca ggttggcctg ctcagcgtag attacgaaag
     5341 gatagattac cggaatattc gtatgcgcaa cagcagtggt tatccatttg cagatgtgaa
     5401 cgaagacatc aataaattct tccggttggg atacaaactg cgcgccggaa cagaaatacg
     5461 cctggacaaa catttctgtt tcagagccgg ctacgctttc tatcaatcac cggtggccaa
     5521 ccatattgaa aaagggaata cggttattta cacaccttcc acggctccac agtattttat
     5581 ggaaaaagat gcacacactg catccctggg ctttggttat cgaacaggag tcttcttttt
     5641 tgacttggct tttgttgagc aattcagaac ggaacatttt tatccctatt acaattggaa
     5701 cggggcaggt gaatatgcca acataaaaac caacacagcc aacattgttt gtagtctggg
     5761 cgttaagttt tagagctctt tgagcctttg ttccagccgg taaagttcat ccctatattg
     5821 cgcagcctcc agaaattcca ttttttcgga ggctgtgtac attaatttgc gggtgttttc
     5881 tatcaacttt tccaattgag gacgactcat gtagcggacc acaggatctg tcagaatatt
     5941 aatctcttcc ggttcaatgt aggctttttt caggttagaa tcttcggcag ccctgctgac
     6001 caatacggaa taattgtctt tttggatagc tgtgggtgtg atgttgttta gtttgttgta
     6061 ttcttcctgt ttttttctgc gccgttgggt ttcctcgagg gtaagttgca tactttcggt
     6121 gattttatcg gcgtataaaa tgactttgcc gttgatgtga cgggcagccc ggccggcagt
     6181 ttgagtcaaa gaacggtgat ttcgcagaaa tccttccttg tcggcatcca ggatggccac
     6241 caaagatact tcgggtaaat cgagcccttc acgtaataaa ttaaccccga tcaatacgtc
     6301 aaattgcccc ttgcgcaact tgtccataat gtttactctt tccagggttt ctacatcgga
     6361 atgaatgtat tcacaaagta caccctgttg cttgagatac gctgataatt cttcggccat
     6421 acgcttggtg agtgttgtca ccagggttcg ttcgttgttt tctgctctct ggcggatttc
     6481 ttccatcaaa tcatctacct gatgtttact ggggcgtact accattacgg ggtccaatag
     6541 ccctgtggga cggatcagtt gttctacgat aagtccgccg gatttttcca gttcataatc
     6601 ggccggggtg gcactgacgt aaataacctg agaagtcatg gcttcaaatt catcgaaacg
     6661 caaaggacgg ttgtccaaag cggccggaag tctgaaaccg tattcaacca gattttgttt
     6721 acgggcatga tcaccccccc acatcgctct gatttggggt acggttacat gactttcgtc
     6781 aatgaccatt aaaaaatcgt cgggaaagaa atcgaggagg cagaagggac gcatgcccgg
     6841 ctcgcggccg tcgaaatacc tggaataatt ctctattccc gagcaatgcc ccagttcctt
     6901 gatcatctct atgtcgtaac taaccctttc ttgcagacgc ttagcctcgt aggctttgtc
     6961 gatggactga aaaaaagcga cctgggcagc cagatcgagt tctatctgtc ttatggcctg
     7021 agcctgcctt tctttggtgg ttacaaacag attggcaggg tagatgtcga aacgatcaaa
     7081 ttcagccagt cttctgcctg tcagggagtc gatttgctcg atggcttcga tttcttctcc
     7141 ccaaaaacag acccggacca ccatatcgga ataggccaga aaaatgtcta cattatcacc
     7201 ttttacccga aaattaccgc gtccggggcc tacatcgttt ctcgaataaa gactgtcgac
     7261 cagattgcgc aaaaagacgt tacggctcaa ggcctggcct ttgtgaacac gctggatgtt
     7321 gttgtgaaaa tccacgggat tgcctatgcc aaacaaacaa gataccgagg agactaccaa
     7381 cacatcccta cggcctgata gtaaggaaga agtggccgac aatctcagtt tctcgatttc
     7441 atcgttgatg gccaggtcct tttctatata gaggtcgctg tgaggcaaat aagcttcggg
     7501 ttggtaataa tcgtagtagg aaacaaaata ttcaaccgca ttttccggga aaaaaccttt
     7561 gaattcactg tatagttgag cggcgagtgt tttgttgtgg cttaatacga gggtagggcg
     7621 ttgcgcttgt tccaccacat tggcaatggt aaaagtctta cccgatcccg tcactccaag
     7681 caaagtttgg tgagaaagtc cctgttgaat gccttctgta agtaattgta tggcttcggg
     7741 ctggtctccg gtgggttgat aagtcgaaac aagtttaaac atgagggcag aagataattt
     7801 gcaaaagtag ctttttttaa aagctttgcg aaatctcgtc ctgaaggatt tattattttc
     7861 agaatacaaa aaaaaattgt tccttcgcag gattattcgt caaaaggctg attccaggcc
     7921 gatattgaat ataattatat gttttttaca ggattagcct ctttccttta tattttgtta
     7981 atatgatttg gaacgaaact tatgaatgcc tgagtcgcaa agaaatgcgg gaactacagg
     8041 gtaaacgttt attcaacacc gttgagcgcg tttattacaa ttctcctttt tatagaaaaa
     8101 aaatgcagga gctcggcatc actcccgagg atatcgaatc ggtcgaagac ctggcaaaat
     8161 tacccttcac ggtaaaaaac gatttgaggg atcaatatcc ttttggcttg ttttcctcgc
     8221 ccatgtcaga gattgtcaga ttgcatgcgt cttcgggtac aacaggtaaa cccatagtcg
     8281 gaggatatac gcgtaaagac ctgaatattt ggtccgaagt aatggctaga actttttcgg
     8341 ccggaaattg cgaccggggc gatatgtttc aaattgccta cggttacggt ttgtttacgg
     8401 gagggctggg agctcatgcc ggagctgaac gtattggagc ttctgtgatt cccatttcgt
     8461 cgggtaatac cgagaagcaa attcagctta tgaccgattt tggaaccacc gccttgtgct
     8521 gcaccccgtc gtatgctcta tacctggcag aaatgat
//
LOCUS       DAOSGL010000061         8429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7193_length_8429_cov_4.209219, whole genome shotgun sequence.
ACCESSION   DAOSGL010000061 DAOSGL010000000
VERSION     DAOSGL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7193_length_8429_cov_4.209219"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKY68_03160"
     CDS             complement(<1..897)
                     /locus_tag="PKY68_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH24068.1"
                     /translation="MEYNFSAIEKKWQEEWIKRQTYKVYEDQSKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIVARYKRLQGYNVLHPMGYDAYGLPAEQYAIQTGQHPAITTAN
                     NIERYREQLDKIGFSFDWSREVRTCEPKYYKWTQWAFIQMFKSYYNTELNRARPIEEL
                     IELFELHGNKDIPAYGGEELPEFSAAQWKSFDDLKQQELLMHYRIAYLGDSMVNWCPA
                     LGTVLANDEVSEGLSLRGGYPVEQKLMRQWCLRISAYAERLLQGLEKIDWTDSLKETQ
                     RNWIGRSEGAEMNFGVKDSDLTI"
     gene            1024..2622
                     /gene="nadB"
                     /locus_tag="PKY68_03165"
     CDS             1024..2622
                     /gene="nadB"
                     /locus_tag="PKY68_03165"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOH24069.1"
                     /translation="MVRKFDFLVIGSGLAGMSFALKVADKGTVAICCKSALEEANTFY
                     AQGGIASVTNPATDNFQKHIKDTLICGDGLCDKSVVEKVVKEAPAQIAELIHWGVAFD
                     KDQSGHYDLHREGGHSENRILHHQDATGAEIQRSLIRRIQQHPNIQVFDHHFAIEILT
                     QHHLGATITRQSEHIECYGAYVLNRRTRAIHTFLAKVTMMATGGIGNIYQTTTNPVIA
                     TGDGIAMVYRAKGQVKDMEFVQFHPTALYHPGERPSFLITEAMRGYGAVLRTRDGEEF
                     MQKYDDRASLAPRDIVARAIDTEMKIRGTDYVYLDVTHKEPAETKKHFPNIYKKCLSI
                     GVDITKEYIPVAPAAHYLCGGIEVDKNAESSIDRLYASGECARTGLHGANRLASNSLI
                     EAIVYADSAAKHAVGRLKDVGFEERIPLWNDEGTRLPEEMILITQSAKEVGQIMSSYV
                     GIVRSNLRLKRALDRLEILYQETERLFIRSVVSKEICELRNIINTGYLVIKQAQLRKE
                     SRGLHYTIDYPNKNELGHTYPLNS"
     gene            2629..3408
                     /locus_tag="PKY68_03170"
     CDS             2629..3408
                     /locus_tag="PKY68_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH24070.1"
                     /translation="MSTMLFQDIIFGPIKSRRLGISLGINLLPLSTKFCSFDCLYCEC
                     GLGDIKNRGILPKTEQVIAALDLKLQELLAKGVVPDVLTFAGNGEPTLHPDFPAIVDA
                     VVVLRDRVVPQAKVSVLSNSSRIDKAEIRAALLKVDNNILKLDSALESTVQWLNRPVK
                     KTYSIRQQIEYMQLFEGKLIVQTLFLRGTFLGKIIDNTTEEEITAWIEALRKIKPLSV
                     MIYTLDRETPVQSLEKIPLKELETIARRLRDATGIPSTVAG"
     gene            3411..4136
                     /gene="mnmD"
                     /locus_tag="PKY68_03175"
     CDS             3411..4136
                     /gene="mnmD"
                     /locus_tag="PKY68_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOH24071.1"
                     /translation="MKQPNEFIPAKSCEVCPTADGSQTLYSNSVQEHYHSTFGALQES
                     RHIFIQAGLLEAYRRKTAGTNPSLKVFELGLGTGLNALLTILEAGKLQLNLSYDCIEA
                     YPLSPDLIKALNYPALFSEQDQNKAAAVLDFIHSAKWNVRHTEPASHTTFTKFRGDFA
                     LFELPQSYDLVYWDPFSPEKQAGMWTEELFASMYKHMTTGSILVSYCAKGEIRRRLQR
                     AGFGVERLQGPPGKREIIRATKE"
     gene            complement(4193..5200)
                     /locus_tag="PKY68_03180"
     CDS             complement(4193..5200)
                     /locus_tag="PKY68_03180"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOH24072.1"
                     /translation="MSRRNYIRHLSGTNFLWVVGLLLCVLGVVFSTMLLTPGSEVLYS
                     DEVVSPFWELIRGFIPGEWLPVLIQFLLLVANAILFQFIVRRYELLRTLGVLTFFIYC
                     FITAASIPLHRLIPGSFAALFIGLAYFSVFESYRKENCNNQVFNASFLIVLAALFVPP
                     LIFLLPLFWLAFNIVNTMNFKRWLVSLMGALTVFWLLGGVLFLSDRLELIPAFFKACI
                     QYGWVFSAELPTMGIVFFSMLFLLVMLAIVNYLRQVYSEKIGVRSAFSFLLISLLVFV
                     VSMVIFPAYTVDFFLLSIGPASVMLSHYYGSEVNLFGRLALYVFLLTAVGNYLSFFFS
                     F"
     gene            complement(5234..6502)
                     /gene="purD"
                     /locus_tag="PKY68_03185"
     CDS             complement(5234..6502)
                     /gene="purD"
                     /locus_tag="PKY68_03185"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOH24073.1"
                     /translation="MKLLLLGSGGREHALAWKLVQSPKVERLFIAPGNAGTSEVGVNV
                     PLAVDDFVAIRDFALKEQIDMLVVGPEIPLVKGISDFFASDPLTAGIPVIGPSKSGAR
                     LEGSKDFSKAFMKRHNIPTAGYLSVTAENVQEGLDFMKKLRPPYVLKADGLAAGKGVL
                     IIQDLEQAQKELKTMLDGKFGEASKTVLIEEFLSGIECSVFVLTDGSQYKILPEAKDY
                     KRIGEGDTGLNTGGMGSVSPVPFADKLFMQKVEERIIRPTIEGLRKENIDYKGFIFFG
                     LIKVDEEPYVIEYNVRMGDPEAETVVLRIKSDLVDLLEGVARGNLDEKKIEFDPRTAV
                     AVMLVSGGYPGDFEKGKEITGLDKVKESIVFHAGTKIQDGKILSSGGRVLAVSSYGNN
                     IAEALSLSFENARKIHFENSYFRSDIGFDL"
     assembly_gap    6632..6641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6642..7049)
                     /locus_tag="PKY68_03190"
     CDS             complement(<6642..7049)
                     /locus_tag="PKY68_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH24074.1"
                     /translation="MKKLAFLLVFSLLLVGAYGQSATNEVINTILNGYSARAYTSEPV
                     SDKDIELILDCGVKAPSARNAQPWKFTVIKNGDLVGQIIRNATPGNVVILISGPESAE
                     QGMSVNIDCALATESMFIAALGLGLGARIYTAPI"
     gene            7458..7862
                     /locus_tag="PKY68_03195"
     CDS             7458..7862
                     /locus_tag="PKY68_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24075.1"
                     /translation="MKTCLLILFAILFSSCVKQEDSTTNGMYQGKIVGYLKCSDNETD
                     NTLLGIFIITNKEDSLLSFNVPTAIYDLDTSNLDNGINFLDGDSVQFSYKNANSEEQK
                     VFDCPPSTMLNPTFYPIENFTQVIITDINKIQ"
     gene            7865..>8429
                     /locus_tag="PKY68_03200"
     CDS             7865..>8429
                     /locus_tag="PKY68_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24076.1"
                     /translation="METFKLNLKIITIASIFGMFTLSFNSEQDKTENPFYYAFDEKVQ
                     IDQLADKLLVKKKPALQKSSYEESTKQLFGEVEIEWLNTDISKVNVKDVKNIEEKINK
                     LLSDDDVISVRKVYKTKDNLEFGFADEIIIKFKDEIQESIREEILKTYNLKKSKTTKI
                     YESVLISKNEDIIEVANTHGGDFDYINE"
BASE COUNT         2515 a         2069 c         1749 g         2086 t
ORIGIN      
        1 gatggtcagg tctgaatctt tgaccccaaa gttcatttcg gctccttcgg aacgacctat
       61 ccaattgcgc tgggtttctt taagcgaatc ggtccagtcg attttttcca aaccttgcag
      121 caaacgttcg gcataggccg aaatgcgcaa gcaccactgg cgcataagtt tttgttccac
      181 cgggtaccct cctcgcaacg acaagccttc gcttacctcg tcgttggcta aaaccgtgcc
      241 taaagccgga caccaattca ccatgctgtc gcccaggtag gcaatgcggt agtgcatcag
      301 caactcttgt tgcttgaggt catcaaagct tttccactga gcagccgaaa attcaggcaa
      361 ttcttcacct ccgtaagcgg ggatgtcttt attgccgtgc aactcaaaga gctcaatcaa
      421 ttcttcaata ggccgcgccc gattcaactc cgtattgtag tagctcttaa acatttgaat
      481 gaaagcccat tgagtccact tataatactt aggctcacag gttctgactt cgcggctcca
      541 atcgaaactg aaaccgattt tatcgagctg ctccctgtaa cgctcaatgt tgttggctgt
      601 agtaatggcc ggatgctggc ctgtttgaat ggcgtactgt tcagcgggca ggccgtaagc
      661 atcgtaaccc ataggatgca acacattgta tccctgcaat cgcttatatc ttgccactat
      721 gtcggaggct atatagccca gggggtgccc cacgtgcaaa ccagctcccg aaggataagg
      781 gaacatatcc agcacatagt atttaggttt agattgatct tcgtagactt tgtacgtttg
      841 acgcttaatc cactcctcct gccacttctt ttcaatggcg ctgaaattat actccatttt
      901 cgcattctaa aaaacgctgc aaaggtacta aagatttgat gaaaaaaaga ttacttttgc
      961 agtccaattt cctgcgataa gtaggttatt tcggattttt gagccacaaa caatggtgtt
     1021 gctatggtac gcaaatttga ttttttggta atcggctccg gcctggccgg aatgagcttt
     1081 gctttgaagg tggccgacaa agggacggtg gccatttgct gcaaatcggc tttggaagaa
     1141 gccaacacct tttacgccca gggcggtatt gcttccgtta ccaatcctgc aaccgataat
     1201 tttcaaaaac acataaagga caccctgatc tgcggagacg gactatgcga taaatcggtg
     1261 gtggaaaagg tggtaaagga ggctcctgcc cagatcgccg aactaatcca ttggggagtt
     1321 gcctttgaca aagaccagag cggacattac gatcttcatc gcgaaggagg ccattccgaa
     1381 aaccgcatcc ttcaccacca ggacgctacc ggagccgaaa ttcagcgcag cctgatccgt
     1441 cgcatccaac aacatcccaa tatccaggtt ttcgaccacc attttgccat cgaaatactg
     1501 acccaacacc atttgggagc caccataacc aggcaatccg aacatatcga atgttacgga
     1561 gcttatgtgt tgaaccggcg taccagagcc atacatactt ttttggccaa agtcaccatg
     1621 atggccaccg gaggcattgg taatatttac caaaccacca ccaatcccgt gattgctacc
     1681 ggcgacggca ttgccatggt ttaccgggca aaagggcagg taaaagatat ggagtttgtg
     1741 cagtttcatc ctaccgccct ttatcatccg ggcgaaagac cctctttttt aattacggaa
     1801 gccatgcggg gttacggcgc cgtattgcgt acacgtgacg gagaggagtt tatgcaaaaa
     1861 tacgacgaca gggcttcgct ggctccccgc gatatcgttg cccgcgccat cgacaccgaa
     1921 atgaaaatcc ggggtaccga ttacgtttat ctggacgtta cccataaaga gccggcagaa
     1981 accaaaaaac attttcccaa tatctacaag aagtgcctgt ctatcggcgt tgacattacc
     2041 aaagagtata ttccggtggc tccggctgcc cattatttat gcggcggcat cgaggtcgac
     2101 aaaaacgccg aatcgagcat agaccgcctg tatgcctcgg gcgaatgcgc cagaacagga
     2161 ctgcacgggg caaaccggct ggcttccaat tctctgatag aagccatcgt atacgccgat
     2221 tcggctgcca aacatgctgt gggacgcctg aaagacgtag gtttcgaaga aagaattccc
     2281 ctgtggaacg acgaaggaac ccggctaccc gaagaaatga ttctgattac ccaaagcgcc
     2341 aaagaagtgg gccagattat gagctcctat gtgggcattg ttcgctccaa cctccggttg
     2401 aagagggctt tggaccgtct ggaaatactc tatcaggaaa ccgaaaggct ctttatacgt
     2461 tcggttgttt ccaaagaaat ttgtgaactc cggaacatca tcaacaccgg ttatctggta
     2521 atcaagcagg ctcagttacg caaagagagc cggggcttgc attataccat cgactacccg
     2581 aacaaaaacg aactgggaca tacttatccc ctaaactcct aaaccgccat gtccaccatg
     2641 ctgtttcaag atattatatt cggtccgatc aagagccgac gactcggtat ttcactgggt
     2701 ataaacctct tacccttgag cacaaagttt tgcagtttcg actgcctgta ttgcgaatgc
     2761 gggctgggcg acataaaaaa caggggtatt cttccgaaaa cagaacaagt aattgccgct
     2821 ctcgacctca aacttcagga attactggcc aagggagttg ttcccgatgt ccttactttt
     2881 gccggtaacg gagaacccac cttgcatccg gactttccgg ccatcgtcga tgcagttgtt
     2941 gtgttgcggg atcgtgtggt tcctcaagct aaagtcagcg ttttgtccaa ttcaagccgg
     3001 atagacaaag cagaaatcag ggcagctctg ttaaaggtgg ataacaatat tctaaagtta
     3061 gattccgctt tggaatcgac tgtacaatgg ctgaaccggc cggttaaaaa gacttattcc
     3121 attcggcaac aaattgaata tatgcagctc tttgaaggga aattgatcgt tcagactctc
     3181 tttctgcgcg gaaccttcct gggcaaaatc atagacaaca ccaccgaaga agaaatcact
     3241 gcctggatcg aagccctgcg caaaatcaaa cccctttcgg tgatgatcta caccctcgac
     3301 agagaaaccc cggtgcaaag cctcgaaaaa attccactga aagaactgga aacgatcgcc
     3361 cgtcgactgc gggatgcaac cggtattccc tctacagtgg ccggctgagc atgaaacaac
     3421 caaacgaatt cattcccgct aagtcctgcg aagtctgccc gactgccgac ggctcgcaaa
     3481 ccctgtattc aaacagtgtg caggaacatt atcattctac ttttggcgcc ctgcaggaat
     3541 cacgccatat ctttattcag gccggcctgc tcgaagcata ccgccgcaaa accgccggaa
     3601 caaatccgtc cttgaaggtt tttgagctgg gcctgggaac cggcctcaac gccttgctaa
     3661 ccatactgga agccgggaag ctgcaactga acttgagcta cgattgcata gaagcctacc
     3721 ctctatctcc cgacctgatc aaggctctca actatccggc cctgttttcg gagcaagatc
     3781 aaaacaaggc tgctgccgtt ctcgatttca ttcattccgc gaaatggaat gttcgccaca
     3841 cagagccggc ttcacataca acattcacca agttccgtgg agattttgcc ttgttcgaac
     3901 tgccgcagag ttacgacctg gtgtactggg atcctttttc gcccgaaaaa caagccggaa
     3961 tgtggacaga agaactcttt gcctccatgt ataaacacat gacaacaggg agcattctgg
     4021 tcagttattg cgccaaaggc gaaatcagaa gaagattaca aagagcaggc ttcggcgtgg
     4081 aaagactgca agggcctccg ggaaaacgcg agatcatccg ggccaccaaa gaataatgta
     4141 cccctgctca gaaagcggaa aaataccgga gaactgcaag ccaagagaac gatcagaagg
     4201 aaaagaaaaa agacaaataa ttgcccacgg ccgtcaaaag aaaaacatat aaagccaaac
     4261 ggccaaacaa gttcacttcc gacccataat aatgagacaa catgaccgat gcggggccta
     4321 tgcttaataa aaagaaatcg actgtataag ccggaaaaat aaccatggaa acaacaaaga
     4381 ccaacaagga gatcagcaaa aaggaaaaag ccgaacgcac accgattttt tcggaataca
     4441 cctgccgcaa gtagttgaca atagccaaca taaccagcaa aaaaagcata gagaaaaaaa
     4501 caatgcccat agtaggcaac tcggcagaaa acacccaacc gtattgtata catgccttaa
     4561 aaaaggccgg aatcagctcc aaccgatcgc tcagaaacaa gactccgccc agcaaccaaa
     4621 acactgtcaa agcacccatg agcgacacca gccatctttt gaagttcatg gtattgacta
     4681 tattgaaagc cagccaaaac aagggaagca aaaaaataag cggaggaaca aacaaagccg
     4741 ccaacacaat caaaaaagaa gcgttgaaga cctgattatt acaattttct ttacggtaac
     4801 tctcaaaaac actgaagtaa gccaaaccta tgaacagggc ggcaaaagaa cccggaatca
     4861 ggcgatgcaa gggtatactg gctgcggtaa taaagcaata aataaaaaaa gtaagcacgc
     4921 ccaacgtgcg cagcaattcg tatctgcgaa caataaattg aaacaggatc gcattggcca
     4981 ccaacaaaag gaattgtatc aacaccggca accactctcc cggtataaaa ccgcgaatca
     5041 gttcccaaaa cggactgaca acctcgtccg aatacagcac ttcactcccg ggcgtaagca
     5101 gcatggtact aaagaccacc cccagcacac aaagcaacag accgacgacc caaaggaaat
     5161 tggtacccga aagatgccga atataatttc ttctgctcat actcaaaaac taaaaacaag
     5221 cggccggttt gctctataaa tcgaagccta tgtcggaacg gaaataagag ttttcaaagt
     5281 gaatcttgcg ggcattttcg aaggatagtg ataaggcctc tgcaatattg ttgccgtaag
     5341 agctgacggc cagcaccctg cctcccgagc tcagtatctt tccgtcctga attttggtac
     5401 cggcgtggaa cacaatgctt tctttcacct tgtccaatcc ggttatttct ttgccttttt
     5461 cgaagtctcc cggatagcct ccagaaacca gcatcacggc aaccgccgta cgcgggtcga
     5521 attctatttt tttctcatcg agattgcccc gggccacccc ctccagcaaa tcaaccaaat
     5581 cggatttaat cctgagtacg acggtttcgg cttctggatc gcccatacga acgttgtatt
     5641 caatcacata aggttcttcg tctaccttga tcaatccgaa aaagataaaa cctttataat
     5701 cgatgttttc cttacgcaaa ccctcgatgg taggacgaat aattctttcc tccacttttt
     5761 gcataaaaag cttatcggca aaaggtacag gcgaaacaga acccatgcct cccgtattca
     5821 gtccggtgtc gccctcgcct attctcttgt aatctttggc ttcgggcaaa attttgtatt
     5881 gactaccatc ggtaagaaca aaaaccgaac attctatgcc gctcaaaaat tcttcaataa
     5941 gcaccgtctt gctggcctct ccaaacttgc catcgagcat ggtttttaat tctttttggg
     6001 cctgttcgag atcttgaata atcaaaacac ccttgccggc agccaaaccg tcggccttga
     6061 gtacgtaggg aggccgcagc tttttcataa aatcgagccc ctcctgtaca ttctctgccg
     6121 tcacactcaa atagcctgcg gtaggaatgt tgtgcctttt cataaaggcc ttggaaaaat
     6181 ctttgctgcc ctccagacgc gccccgcttt tggaaggacc gataaccgga ataccggccg
     6241 ttagcggatc ggaggcgaaa aaatcgctga tgcctttaac cagaggaatc tccggcccaa
     6301 ccaccagcat atcgatctgt tctttgagag caaaatcccg gatggcgaca aaatcatcta
     6361 ccgcaagagg cacattgact cccacttcgg aagtaccggc attacccggc gcaataaata
     6421 aacgctctac ttttggactt tgaacaagct tccaggccag cgcgtgctca cgccccccgg
     6481 aacccaacaa caataacttc atccttttct gcttattcca ccacatttac cagctcagct
     6541 ttcgccttcc gcggtgaagc cgccgtaacc gcatcaacat tcttatccat atgcccaatc
     6601 ctcaaggcgg taactgccct gtagccttcg gnnnnnnnnn ngataggtgc tgtgtatatg
     6661 cgggcgccca ggcctaggcc caaagccgcg ataaacatac tttccgttgc caaagcgcaa
     6721 tcgatattca cactcatacc ttgttcggcc gattcgggtc cggaaatcag gatgaccaca
     6781 ttgcccggag tggcattgcg aatgatttgc ccaaccagat ccccgttttt gatgacggta
     6841 aatttccagg gctgggcgtt tctggcgctg ggagctttta ccccgcaatc gagtatcaat
     6901 tcgatatcct tatccgaaac cggttccgag gtatatgccc tggccgaata gccattcaat
     6961 atggtattaa tcacttcatt ggttgcactc tgtccatacg cgccaacgag caacaagcta
     7021 aaaactaaaa ggaaggccaa ttttttcata atgttactgt ttaaaaatac agctcaaaga
     7081 tattcatttc ttccaactaa tttcagtagt ccgtccggaa agttcatttt acccttccaa
     7141 cagcgtaggc ccgttctcaa attacgctgt cagagtcttg caaattgtta ttcattatta
     7201 acttttgccc cgatattgtt aacattttaa ctcagctttt gtattaatct tttatttcct
     7261 ttgagttgaa tttagttgct ccctgaacta ttttgtaatg tataactaaa ctctaagact
     7321 ctcttataga atcttttaaa tcttgtgcca tgaaatagtt ctcttatctc tatttgcttg
     7381 ttagagccaa tacaatgaaa catagcagct acatatcaac cgagataaag atgtttaaca
     7441 attaaaaatt taaaaacatg aaaacgtgtt tattaatctt atttgcaatc ttgttttctt
     7501 catgtgtcaa acaagaggat tccactacca atggtatgta tcaaggtaag attgttggtt
     7561 acctaaaatg ctctgataat gaaactgata atactctttt gggtattttc ataattacga
     7621 acaaagaaga ttctttatta tcatttaatg tccctacggc aatttatgat ttggacacaa
     7681 gtaacttaga taatgggatt aattttttag atggagattc tgttcagttt agttacaaaa
     7741 atgcgaacag cgaagaacaa aaagtatttg attgtccgcc atcaacgatg cttaatccta
     7801 ctttctatcc tattgagaat tttacacaag taataataac cgacataaat aaaattcagt
     7861 aaccatggaa acttttaaac tcaacttaaa aattattacc atcgcaagta tttttggcat
     7921 gtttacatta tcctttaatt cagaacagga taaaactgaa aacccttttt attacgcttt
     7981 cgatgagaaa gtgcaaattg accaattggc tgataaatta cttgttaaga aaaaaccagc
     8041 attgcaaaag tccagctatg aggagtctac aaaacagctc tttggcgaag tagaaattga
     8101 atggttaaat acggatatta gtaaagtcaa tgtaaaggat gtcaaaaaca tagaagaaaa
     8161 aattaacaaa ctactgtctg atgatgacgt aataagcgtc agaaaagttt ataaaacaaa
     8221 agataattta gagtttggtt ttgcagacga gataattata aaatttaaag acgaaataca
     8281 agaatcgatt cgggaagaaa tactgaaaac gtataatcta aagaaatcca aaaccacaaa
     8341 gatttatgaa tctgttctaa tatctaaaaa tgaagatatt attgaagttg caaatactca
     8401 tggcggtgat tttgattata tcaatgaac
//
LOCUS       DAOSGL010000062         8422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7204_length_8422_cov_4.017330, whole genome shotgun sequence.
ACCESSION   DAOSGL010000062 DAOSGL010000000
VERSION     DAOSGL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7204_length_8422_cov_4.017330"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /gene="trkA"
                     /locus_tag="PKY68_03205"
     CDS             <1..1135
                     /gene="trkA"
                     /locus_tag="PKY68_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOH24077.1"
                     /translation="FIAVTPEESRNLTACMLAAKLGVKKTVARVENPEYLHPDQKAFF
                     EQLGIDSLICPEILAAKEIADSLVRPWVRQWHEFNNGTLILVGVKVREGAKIIGKELM
                     SLFTHNESCRIAAIKRGKDTIIPGGRDIIQTDDMVYFITTREHVDTVRKMADKPHTEV
                     HKVMIMGGSRIAMLTAERIAEQFDVKILEVNKERSEKVKERLGSKAMVILGDGRDIDL
                     LHDEGIEQVEAYIALTGNSETNILSCLAAKRFQVQRTVAEVENNDYIPLAEGLDIGIV
                     INKKLIAAGHIYQMMLDADVSNMKCLTFADAEVAEFVVKEGAKITKSKVMDLRLPENI
                     GIGGLTRDDKGIIVYGNTQILPGDRVVLFCPSTYLRKVEKFFK"
     gene            1145..2599
                     /locus_tag="PKY68_03210"
     CDS             1145..2599
                     /locus_tag="PKY68_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOH24078.1"
                     /translation="MPKSLFNIRIILRFLGHLLILEAMLMLICGGIDFLFKDHTQLVF
                     LQAAGLTFCAGLFLALNGMHAKRDIGKHESFVIVTLVWVLFSLFGALPFYLSGHFNSF
                     TDAFFESMSGFSTTGSSVLVDVEILSKGILFWRSLIQWLGGMGIILLSLAIMPMLKIG
                     NYQLLTAEVPGPVYDKIHPRVTVAARRLWFLYSGLTILLVVLLRLGGMSLFDSICHSL
                     TTMATGGFSTKNASIAHWDSAYIEGVTVLFMFLAGTSFVLLYRVFSGKFYKIKQNEEF
                     KAYFYIVLVSGLVLSTALFFFADLSPVKALRTGFFQVVSIITTTGFTTADFSVWVPGI
                     RFLLIVTMFFGAMSGSTSGNIKIGRIVLYTKISYNELKRDVHPNAVLPVFFNGRAMPA
                     TLIYGLFGFLFFYILLMILSVGIFFAMGLNMEDSLGVFLTSFGNIGPGLGNHFSNFFD
                     LPGIAKWYSGFLMLVGRLEIYTVFLLFSRTFWRN"
     gene            2671..4053
                     /locus_tag="PKY68_03215"
     CDS             2671..4053
                     /locus_tag="PKY68_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOH24079.1"
                     /translation="MFLCLPALLACNRKTGEMDLAFVQAAQIEKNIKQTSFPDRSFSI
                     LDFGAKAGDSCFLNHEAINNAILECSLNGGGIVLIPAGEFYTGPIRLKSNVNLRLEKE
                     ATLKFSTDTSLYLPQVLTRWEGVDCYNYHPLIYAYGETNIALTGEGVLDAQGQKGWWS
                     MCGARKYGWTEGLPGQNQGGRNRLLRSAEEKLDISQRIMTPQDALRPQMVNFYRCKQV
                     LIEGITLLNSPFWVLHPLLCEDLTVRGVHIYNRGPNGDGCDPESCKNVLIEDCFFDTG
                     DDCIAIKSGRNNDGRKWGVPSENIIVRNCKMKNGHGGVVIGSEISGGYRNLFVENCDM
                     DSPDLDRVIRIKTNTCRGGLIENIFVRNIKVGQCREAVLKINLLYESREDCQRDFPPT
                     VRNVQLENITCEKSRYGVLIAGLPGDENVYHIRLENCQFNGVERGNSINGARDVSFKN
                     LHINGELVEE"
     gene            complement(4145..5779)
                     /gene="groL"
                     /locus_tag="PKY68_03220"
     CDS             complement(4145..5779)
                     /gene="groL"
                     /locus_tag="PKY68_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOH24080.1"
                     /translation="MAKEIKFDIEARDLLKSGVDQLADAVKITLGPRGRNVILEKKFG
                     APQITKDGVTVAKEIDLADAYENTGAQLVKNVASKTGDDAGDGTTTATVLAQAIIREG
                     LKNVTGGVNPMELKRGIDKAVAGVVASVKSQSRVVGDDFEKIEQIARISANNDAEIGR
                     LIAEAMRKVKKEGVITIEEAKGTDTRIDVVEGMQFDRGYLSPYFVTNTEKMEAELENP
                     YILIHDKKISNLKELLPILESAVQSGRPLLIIAEDIDGEALTTLVVNRLRAGLKICAV
                     KAPGFGDRRKEMLQDIAVLTGGVVITEEQGIQLESATLDMLGNAEKVTIDKDNTTIVN
                     GSGAKEAIADRVNQIKAQIAATTSDYDREKLQERLAKLSGGVAVLYVGAPSEVEMKEK
                     KDRVDDALSATRAAIEEGIVPGGGVAYIRALDAIASLKGENEEEDLGIKIIRRAIEEP
                     LRQIVTNAGKEGAVIVQKVKEGKNDFGYNARTDKYENLFSTGVVDPAKVARVALENAA
                     SIAGMFLTTECVVVEKKEESPAPAMNPGMGGMGGMM"
     gene            complement(5833..6102)
                     /locus_tag="PKY68_03225"
     CDS             complement(5833..6102)
                     /locus_tag="PKY68_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOH24081.1"
                     /translation="MTLKPLSDRVLIKPAPAEQKTAGGIIIPDSAKEKPLKGEVIAVG
                     PGTKDEAMQLAVGDSVLYGKYSGTEIELDGEKYLIMRQSDILAKF"
     gene            complement(6274..7782)
                     /locus_tag="PKY68_03230"
     CDS             complement(6274..7782)
                     /locus_tag="PKY68_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOH24082.1"
                     /translation="MKKIVSFMFCAVLILAAHAEIRLPNVLTSNMVLQQGKAVHIWGK
                     ADPGATLKVSFAGQEKETPVNADSTWILSLDPMEASFEPRILTLQLVKESAETEEIQL
                     NNILVGEVWICGGQSNMEYTMDLSLKRYAVPGRGADLADQEWRSGGNKQIRLMRVEKV
                     LSLPDCTTKGWLECADTTLAPFSAAAYYFGKQIQEKLNVPVGLIGSYWGGSRIEAWIP
                     EKEYKAAAFYQTECSQHPTVNRSIGHFYKSMIEPLAPYTLRGAIWYQGESDAMLHDSL
                     YVEKFQLMTTVWRTIFKQEEMPFYYVQIAPYLYTNRKRDPYPHTPQTMAEFCALQTLC
                     LEQAFTGQAIVTDLVDNLSDIHPSYKWEVGRRLALWALAKDYGYEKLVYSGPVLKSAR
                     LCKNKIILRFDHAGSGLTAGERDAQDQFKTLKRATNLSWFELECEGGSVVTAKARVAR
                     SWWFFGKSRNKVKVILPQGTKVNALRFAWDEKARPNFYNKEGLPAVPFRHTF"
     gene            complement(8037..8123)
                     /locus_tag="PKY68_03235"
     tRNA            complement(8037..8123)
                     /locus_tag="PKY68_03235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8087..8089),aa:Ser,seq:cga)
     gene            complement(8236..>8422)
                     /locus_tag="PKY68_03240"
     CDS             complement(8236..>8422)
                     /locus_tag="PKY68_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOH24083.1"
                     /translation="SVNCRILTLGQYLQPTPRHYPVKAYIHPDDFERFREIALEKGFK
                     KVASAPLVRSSYLADEL"
BASE COUNT         2162 a         2028 c         1920 g         2312 t
ORIGIN      
        1 ctttatcgct gttactcccg aagaaagccg caacctgaca gcctgtatgc tggccgccaa
       61 actgggagta aaaaaaacgg tggccagggt tgaaaacccc gaatatctgc accccgacca
      121 aaaagccttt tttgaacaat taggcataga ctcccttatc tgtcccgaaa ttctggcagc
      181 caaagaaatt gctgacagcc tggtccgccc ctgggtgcgt caatggcatg aatttaacaa
      241 cggtacactg atcctggtcg gtgtcaaagt aagagaagga gccaaaatta taggaaaaga
      301 actgatgagt cttttcactc acaacgaatc ctgccgtatt gctgccataa aaagaggcaa
      361 agacaccatt ataccgggtg gacgcgacat aattcaaacc gacgatatgg tttattttat
      421 caccaccaga gaacatgtag acacggtacg aaaaatggcg gacaaacctc ataccgaggt
      481 acacaaggtt atgatcatgg gcggcagccg tatcgccatg ctcactgccg aaagaattgc
      541 cgaacaattc gatgtgaaaa tactggaagt aaacaaggag cgttccgaaa aggtaaaaga
      601 aagactggga tccaaagcca tggtaatcct gggcgacgga cgggacattg acttacttca
      661 cgacgaaggc attgagcaag tggaagccta cattgccctg accggcaact ccgaaaccaa
      721 tattctttcc tgcctggcgg ccaaacgttt tcaggtacaa cgcacagtgg cggaagtcga
      781 aaacaacgat tacattccgc tggctgaagg cctcgacatc ggtatagtga tcaacaaaaa
      841 attgattgcc gcaggacata tttatcaaat gatgctcgat gcggatgtct cgaatatgaa
      901 atgtcttact tttgcagacg ccgaagtagc tgaatttgta gtcaaggaag gcgccaaaat
      961 aaccaaatcc aaagtaatgg atcttcgttt acccgaaaat atcggtatcg gaggactcac
     1021 ccgcgacgac aagggaatta tagtttacgg aaacacacag atactacctg gcgacagagt
     1081 cgttttattt tgccccagca cctacctgcg taaagtggag aaatttttca aataaattga
     1141 ccggatgcca aaatcgctgt tcaatattcg tattatttta agattcctgg ggcatctgct
     1201 gatactggaa gcaatgctga tgctgatatg cggcgggata gactttttgt ttaaagacca
     1261 tacccagctt gtatttttgc aagcagccgg ccttaccttt tgtgcaggac tgttcttagc
     1321 attgaatgga atgcacgcca aacgagatat tggtaaacac gaaagttttg taattgttac
     1381 cctggtttgg gttttatttt ccttgttcgg agccctgcct ttctacctga gcggccattt
     1441 caacagcttt acggatgcct ttttcgaaag catgtccggt ttcagcacaa caggctccag
     1501 tgtcctggtc gatgttgaaa tcctttcaaa ggggatactt ttttggcgga gtctgataca
     1561 atggctgggc ggtatgggca ttatattact ttcgctggcc ataatgccta tgctcaaaat
     1621 aggcaattat caattgctta cagccgaagt tcccggtccg gtctacgata aaattcatcc
     1681 cagagtcacg gtggctgccc gtcgtctgtg gtttctttac tcaggattaa ccattctatt
     1741 ggttgttttg ctgcgacttg gaggaatgag tttattcgat tccatctgtc actctttaac
     1801 aaccatggcc acaggtggct tctccaccaa aaatgccagt attgctcatt gggactctgc
     1861 ctacatcgaa ggggttaccg tgttgtttat gtttctggcc ggcaccagct ttgtcctttt
     1921 gtacagggta tttagcggga aattctataa aataaagcaa aacgaagaat tcaaggccta
     1981 tttttatatc gttctggttt cgggcctcgt cttgagtacc gccttgtttt tctttgccga
     2041 tctgtcaccg gttaaagctc ttcgaacagg tttttttcag gtggtatcca taataaccac
     2101 taccggtttt acaacggccg acttcagtgt ctgggttccc ggcatacgct ttttgctcat
     2161 agtcaccatg ttttttggag ccatgtcggg ctcaacctcg gggaatataa aaattggccg
     2221 tatagttctg tacaccaaga tatcgtacaa cgaactaaaa cgcgatgttc atcccaacgc
     2281 ggtgttgccc gtattcttca atggaagagc catgccggcc accttgattt acggcttgtt
     2341 cggctttttg ttcttttata tcctgctgat gatactcagc gtgggcatct tttttgccat
     2401 gggcctcaac atggaagatt ccttaggtgt attcctgacc agcttcggta atataggccc
     2461 gggactgggc aatcacttca gcaacttttt cgacttgccc ggtattgcta aatggtattc
     2521 cggtttcctg atgttggttg gccgtctcga aatttacacc gttttcctcc tattctcccg
     2581 aacattctgg cgcaattgac aacgatccga ttcaatctat taaccaatta aattcttatc
     2641 tcgatgaaac aacattctta ttatttactg atgtttttat gcttgccggc cctgttagcc
     2701 tgcaatcgta aaaccggcga aatggacctg gcttttgtgc aagctgcaca aattgaaaaa
     2761 aacattaagc aaacttcttt ccccgatcgt agcttttcga ttttggattt cggagccaag
     2821 gccggagaca gctgtttttt gaaccacgaa gctatcaaca atgccatcct ggaatgcagc
     2881 ctaaacggtg gaggtatcgt attgatacct gccggagaat tttacaccgg tcccatccgg
     2941 ctcaaaagca atgttaacct gcgtttggaa aaagaggcca cactcaaatt ctcgaccgac
     3001 accagtttat atcttccaca ggttcttact cggtgggaag gagtagattg ttacaattat
     3061 catcccttga tctatgccta cggcgaaacc aacattgccc tgacaggcga aggtgtgctc
     3121 gatgctcagg gacagaaggg ttggtggagc atgtgcggtg ccagaaaata cggctggacc
     3181 gaagggttgc cgggacaaaa ccagggcgga cgcaaccgtt tgctgcgttc ggccgaagaa
     3241 aagctcgaca tcagccaacg cattatgact cctcaggatg ccctgcgacc ccaaatggtc
     3301 aacttttatc gttgtaaaca ggtactcatc gagggaatta ctttgctgaa ttctcctttt
     3361 tgggttttgc atcccctgct ctgcgaagac ctaaccgtca ggggcgtaca catttataac
     3421 cgtggaccca acggcgatgg ctgcgatccg gaatcctgca aaaacgtact tatcgaagat
     3481 tgctttttcg acacaggcga cgactgcatt gccatcaaat cgggcagaaa caacgacgga
     3541 cgtaaatggg gagtcccctc cgaaaacatc attgttcgca actgtaagat gaaaaacggc
     3601 cacgggggtg tggtgatagg cagcgaaatc tccggaggtt accgcaacct gtttgtagaa
     3661 aactgcgaca tggacagccc cgaccttgac cgggtgatcc ggatcaaaac caacacctgc
     3721 cggggaggcc taatcgaaaa tatttttgtc cgcaacataa aagtaggcca atgccgcgaa
     3781 gctgtgttga aaatcaattt actctacgaa tcccgcgaag actgtcaacg cgatttccca
     3841 ccgaccgtac gcaatgtcca acttgaaaac atcacttgcg aaaaaagccg ctacggcgtt
     3901 ctgatagccg gtttaccggg agatgaaaat gtttaccaca tacgtctgga aaattgtcaa
     3961 ttcaacgggg tggaacgcgg caacagcatc aacggagcca gggatgtgtc gtttaagaat
     4021 ttgcacatca atggggagtt ggtcgaggaa taacaataag cgaaactaat aagcaaaaaa
     4081 gagcgtttca caaattgaaa cgctcttttt tacattagaa tttatttatc tgagcaggcc
     4141 aattttacat catgccgccc ataccgccca tacccggatt catggcagga gcgggacttt
     4201 cttctttttt ctcaactacc acacattctg tagtcaggaa catgccggca atggatgccg
     4261 cattttccaa agctacccga gccactttgg cagggtctac tactccggtg gagaagagat
     4321 tttcgtattt gtcggtgcgg gcattgtagc cgaaatcgtt tttaccttct ttgaccttct
     4381 ggacaataac ggctccttct ttaccggcat tggtcacaat ttggcgcaaa ggttcttcga
     4441 tggcacgacg aatgattttg atacctaaat cttcttcttc gttctcaccc tttaaggaag
     4501 cgatagcatc caaggcacga atgtaagcca caccgccacc gggtactata ccttcttcga
     4561 tggcagcccg ggttgcactc aatgcatcgt ccacgcggtc tttcttttct ttcatttcca
     4621 cttcggaggg agcaccaacg tagagtactg ccacaccgcc cgacaactta gcaagacgtt
     4681 cctgcaattt ttcacgatcg tagtcggaag tggtggcggc aatctgagcc ttgatttggt
     4741 ttaccctgtc ggcaatggct tctttggcac cggaaccgtt tacaatagtg gtattgtctt
     4801 tgtcaatggt aactttttcg gcattaccca gcatatcgag agtggcagat tccaactgta
     4861 tgccttgttc ttcggttata accacaccac cggtcaacac ggcgatatcc tgcaacattt
     4921 ctttgcgacg atcgccaaat ccgggagctt tcacggcaca aatcttaaga cctgcacgca
     4981 gacggttcac taccagggtg gtgagcgctt cgccgtcgat gtcttcggca atgatcaaga
     5041 gcggacgacc cgactgaacg gccgattcta aaataggcag cagctccttg aggttcgaaa
     5101 tcttcttgtc gtgaatcagg atatagggat tttccagctc ggcctccatc ttttcggtat
     5161 tggtcacgaa atagggtgaa agatagccgc ggtcaaactg cataccttct actacatcta
     5221 tgcgggtatc ggtacctttg gcctcttcga tggtgataac gccttctttt ttgactttcc
     5281 tcatagcttc ggcaatcaag cggcctattt ctgcgtcgtt attggcagaa atgcgggcaa
     5341 tttgctcaat tttttcaaaa tcgtctccga ctactcttga ttgagatttg acgctggcaa
     5401 cgactccggc tacagccttg tcgataccac gcttgagttc catcggattg actccgccgg
     5461 ttacattttt caaaccttca cggataatag cctgagccag cacagtagca gtggtggtgc
     5521 cgtcaccggc atcgtctccg gttttggaag ctacgttctt gaccagttgt gctcccgtgt
     5581 tttcgtaagc atcggccaaa tcaatttctt tggccacggt aacaccgtct ttggtgatct
     5641 ggggagctcc aaatttcttt tccaaaatta cgttgcgacc tctgggtcca agggtaattt
     5701 taacagcatc agccagttga tcaacgccgc tttttagtaa atcgcgggct tcaatatcga
     5761 atttaatttc ttttgccata atgatagtat taatgttgat tgtttgtttt tgaaaggaat
     5821 cgtaagggca ttttagaatt tagccagaat gtccgattga cgcatgatca aatacttctc
     5881 tccgtcaagt tcaatttctg tacctgaata tttaccgtag agtactgaat cacctactgc
     5941 caattgcatg gcttcgtctt tggttccggg tccaacagca ataacttcgc ctttgagggg
     6001 tttttctttt gccgaatcgg gaataatgat tccgccggcg gtcttctgct ctgcaggtgc
     6061 gggcttaatc agcacgcgat ctgataatgg tttaagtgtc atgttaattt aaaatttata
     6121 gttaacgtat atcgtaaata atttgtgtgc gaactcaaat catactgcga attctatgcc
     6181 aaaagaaatc tcgtgacaaa agttcagcat tttgtcgtaa gcaatcggca caactgacat
     6241 tattgcacaa ccggcagtca gataaagctg tttttagaat gtatgcctga aaggaacggc
     6301 aggtaaacct tctttgttat aaaaattcgg ccgagctttt tcgtcccagg caaaacgcag
     6361 agcatttact tttgtacctt ggggcagaat tacttttacc ttgttgcggc tcttaccaaa
     6421 gaaccaccac gaacgggcca ccctggcctt tgcagtaacc acactgcccc cttcgcattc
     6481 cagttcaaac cagctcaggt tggttgctcg tttcagggtt ttaaattgat cctgagcatc
     6541 gcgttcaccg gcagtcagtc cgctgcccgc gtgatcgaaa cgcaatatta tcttgttttt
     6601 gcacaacctg gcagatttca gcaccgggcc tgaatagacc agtttttcat aaccgtaatc
     6661 cttggccagg gcccacagag ccagtctccg ccctacttcc catttatagg agggatgtat
     6721 gtcgctcaaa ttgtctacca aatccgtcac tatcgcctgg ccggtaaatg cttgctccag
     6781 acatagagtt tgcagggcgc aaaattcggc catagtttgc ggtgtatgcg ggtaaggatc
     6841 tcttttgcgg ttggtatata aatagggagc aatctgcaca taataaaaag gcatttcctc
     6901 ctgcttgaaa atagttctcc aaacagtggt catcaattga aacttttcga catacagcga
     6961 atcgtgcaac atagcatccg attctccctg ataccaaatg gcaccgcgta aggtataggg
     7021 agccagcggc tcaatcatgc tcttgtagaa atggccgatg ctgcgattaa ctgtagggtg
     7081 ctgactgcat tctgtctgat agaatgcggc cgctttgtat tctttttcgg gaatccaggc
     7141 ttcgatacgg ctgcctcccc agtaagatcc tatcagacca accggaacat ttagcttttc
     7201 ctgaatctgt ttaccaaaat aataggcagc agccgagaag ggagccaggg tggtatcggc
     7261 acattccagc catcctttgg tggtacaatc gggtaggctc aagaccttct cgacccgcat
     7321 aagtcttatc tgtttgttgc caccagaacg ccattcctga tccgccaaat cagctcccct
     7381 gcccggaaca gcgtaacgtt ttaaacttaa atccatggta tattccatat tggattgtcc
     7441 cccacagatc catacttcgc ccaccaggat gttgttcaat tgaatctctt ctgtttcagc
     7501 ggactcttta actaattgaa gcgttaaaat tcggggttca aacgaggcct ccatcggatc
     7561 gaggctcagt atccaagtgc tgtcggcatt gaccggggtt tctttttcct gcccggcaaa
     7621 gctaacttta agtgtggcgc cgggatcggc cttgccccaa atatgaacag ctttgccctg
     7681 ttgcaatacc atgttggatg taagcacatt aggcaatctt atttcggcat gggcagccaa
     7741 aatcagtact gcacaaaaca taaatgatac gatttttttc atagtggtaa gtattatttg
     7801 atttttacaa aagtacgatt tttggggaat aattgtgcta attcacttgt tttctgcgag
     7861 cttgcgggga gaagggatta cggcgtcccg ccgtgatacc cattcggagg atcctagcac
     7921 ggaggaaaga cgatgccggg ggcatcggac cggccaaaaa aatcccagcc ctcgcaagca
     7981 agcttgcggg gctgggattt tttgaccggg cgagctttgc tcgcctttcc tccgtgcgga
     8041 aagtgaggga ttcgaacccc cggtacagtc acccgtacac cgcatttcga gtgcggccca
     8101 atcgaccact ctggcaactt tccgagttga gcgcaaaagt acgcttttcc tgtaagttgc
     8161 caaagttttc gggaattttt attgcaaact ataattctcc gttatttaat attttaaaga
     8221 ttctaccgca ctgacttaca actcgtcagc caaataagat gacctaacta aaggagcact
     8281 ggccaccttt ttaaaccctt tttcaagagc aatttcccgg aaacgctcga agtcgtccgg
     8341 atgaatatag gctttgaccg ggtaatgccg gggggtgggt tgcaaatatt gtcccaaagt
     8401 gagtatgcgg caattcaccg at
//
LOCUS       DAOSGL010000063         8419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7208_length_8419_cov_4.107843, whole genome shotgun sequence.
ACCESSION   DAOSGL010000063 DAOSGL010000000
VERSION     DAOSGL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7208_length_8419_cov_4.107843"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PKY68_03245"
     CDS             complement(<1..195)
                     /locus_tag="PKY68_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOH24084.1"
                     /translation="MDNYSQALNWAQHYCSKAERCSGDVLIKLGKYKLDEASTKRLLE
                     SLRKENYLNDLRYAKAYTMDQ"
     gene            complement(188..1039)
                     /gene="prmC"
                     /locus_tag="PKY68_03250"
     CDS             complement(188..1039)
                     /gene="prmC"
                     /locus_tag="PKY68_03250"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOH24085.1"
                     /translation="MQTFFSYAAQSLKEQYRPEEIRQLSYALLEDCCGFSKNDIYAGK
                     DTEISTKQRRQLHEKLGRLAEGEPLQYITGVAYFYDLKLSVNPSVLIPRPETEELVEW
                     IIKDNKGKTGRILDVGTGSACISLALAKHLPGMQVEAWDISPDALALARENAADLGLD
                     VAFIEQDLFRFNSGDRASVYDVIVSNPPYVTTAEALKMEKRVLEHEPALAMFVPDNDA
                     LVFYRELAALAARTLKPGGSLYVEISIYYGREIVQLFEEAGFDEIILRQDISGRDRMI
                     KAAKCHG"
     gene            1038..2174
                     /gene="ribD"
                     /locus_tag="PKY68_03255"
     CDS             1038..2174
                     /gene="ribD"
                     /locus_tag="PKY68_03255"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOH24086.1"
                     /translation="MVNNLFTKIMHFSLLHNTSADKNLEVLYMRRCLQLAANGRISCS
                     PNPMVGALIVHQNTIIGEGWHHSAGKAHAEPRAIKSVKNRELLKESTLYVNLEPCSHF
                     GKTPPCTDLIIEKKIPRVVIACPDPFPGVCGKGIAKLKASGIEVELGLEAEAANYLNK
                     SFFCYYQKQRPYIVLKWAQTADAYSDYIRQPGDGQKPLQISSAYSRMLVHKLRAESDA
                     IMIGSRTALLDNPRLTLRHWPGRHPLRIVMDRKNTLPKDSLLLNDEFPSLIFTENFSD
                     RRGNKEWLALSGHNSGSMLGDMLAVLHARNIRSLLVEGGSTLQKAFLDAGLWDEIQTE
                     TWHHTIKNGHRALRIPEDQLKACSITSTSIASENRTITHYLRKH"
     gene            2274..3614
                     /locus_tag="PKY68_03260"
     CDS             2274..3614
                     /locus_tag="PKY68_03260"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HOH24087.1"
                     /translation="MNKLLYSIGKKVCLLVLLSPVLLGVSSCHSFEDQSFSLANVQHF
                     GFDSVPGITRYVFYVDNFAGLIYNNDSLPYQTRIDSLVPSIVFFSSNGNVFINDTMLW
                     KPGDVLDFSRPLSLTNTSDDGKHQKKYRIRVNTHQINPDSMVMNVLSSRFPTIAARNK
                     TILYNGLFKAFFAPDTGSIISYTSTDQCLTWSADAPVSGLNDTIFIQSLTEFKGQYYL
                     LGKQGRLYYSGDLNQWSGSADSTKVVSLFAKLEGRKFLSEDALVGIVADNNGDLHFAR
                     FEGESEGWECGTAVPENFPLCEYALTKYKTVTDVPYISLLGGKNASGALNGDSWSTMD
                     GLYWIKVNTKPLHIEPRQGASLFHYDDKMFYYGGLDSRGLCDNTLYVSGNNGVTWSAA
                     DTLLQFYSIDGGLAYQDIFVEEGKYIWIFGGESGSNISNKVWKGNIHRKLFPGK"
     gene            3681..4367
                     /locus_tag="PKY68_03265"
     CDS             3681..4367
                     /locus_tag="PKY68_03265"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOH24088.1"
                     /translation="MTGRRAFFSICLLLCSAAMYGQSYRIEIGGLGGLSFYTGDANQT
                     KLFEKVHPAYGVLMRYHLNRRFSIKGNLLSAGISGTTTDKLEQYPGAEALDFSRRLLD
                     GGLQFEFNFYEFGAADYYPGAGNVSPYVLCGIGLTGYKTDKNALTANIPLGLGIKLKL
                     PARFNLGLECTYRMSFSDKLDYTPQSNLFHLDDPWIAQSAWNKNKDGYFVLAFYLSYD
                     LFYIGSKCYK"
     gene            4369..5112
                     /locus_tag="PKY68_03270"
     CDS             4369..5112
                     /locus_tag="PKY68_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOH24089.1"
                     /translation="MSGLEKIDKSTLPKHVAIVMDGNGRWAKSRGKDRIFGHTEGINS
                     VRAVVEAARELGLSYLTLYVFSTENWNRPQKEVNALMRLMTETIDRETDDLIKNGVRG
                     LVVGDLDRLEKDIQDKVQQFMHNTAQGNELNLVLALSYSSRWEIMEAVKSIAKSLSEG
                     RILEQELDEDCFSKHLCTAGIPDPDLLIRTGGEIRISNFMLWQAAYAELYFTETAWPD
                     FRKEAFYEAIIEYQSRERRFGKTSEQLTK"
     gene            5210..7696
                     /gene="bamA"
                     /locus_tag="PKY68_03275"
     CDS             5210..7696
                     /gene="bamA"
                     /locus_tag="PKY68_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOH24090.1"
                     /translation="MPEIYYSYPRELKIADIAVEGEGIDNYDDYVLIGLSGLSVGQII
                     RVPGSETSEAVKRFWRHGLFSDVKIIADKIEGNEIWLRIVLSPRPRISKINYNGMKKS
                     EIEELEPRLNMIVGNQITPNIIDRAEILIKKHYDGKGYKNADVNVLQRDDPESEDQVI
                     IDINVDKQDKVKVNQLIIEGNEAFPDGKVKWTMKKTNEKNKLINFFRTKKFVDTEYLN
                     DKIALIEKYNEYGYRDAVIVEDTVYRFDDNTVNVYLKVDEGRQYFIRDISWVGNTLYS
                     SEELNMRLQIEKGDVYNQKRLNERLHQSPNEDAVTDLYMDRGYLFFQAIPVEVSVAED
                     SIDLEIRLSEGQPASIKRININGNDRLYEHVIRRELYTKPGELFNKAALMRSAREIAQ
                     TGHFDPETMGINPIPNEDGTVDIDLNLTSKSNDQVEFSLGWGSTGIVGSIGLKFTNFS
                     MRNLLRLGEGVLPQGDGQTFSITGRTNGQYYQSYSFSFMEPWLGGKKPNSFSFSGYYS
                     YQTGVSNRYYENYYSNYMMHYATYGNTYDYGDYYLTEMDPTKYIKMWGLSAGLGTRLT
                     WPDDYFHLFSEFSYQHYNLENWMFFLISNGRSNDLSLNFTLSRNSTDNPLYTRSGSSY
                     SLSLGITPPFSLLDNKTDEDYKKMSSQELYNWIEYYKIRFRSRTYTPLSGNRKLVMAT
                     RFDIGLLGAFNQYKRSPFGTFYMGGDGMTGYSSSYAYDIIALRGYGNGDLGVGWLYQR
                     AGLELRYPLLMEQSATIYASAFLEAGNLWSDFKHYSPFELKRSAGVGVRIFIPIVGLL
                     GIDWAYGFDPVGTETKAHGGEFHFVIGQEF"
     gene            7751..8260
                     /locus_tag="PKY68_03280"
     CDS             7751..8260
                     /locus_tag="PKY68_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOH24091.1"
                     /translation="MKKAYLLIISLIFSSMAVFAQKYALVDMEYIFSRIPAYEMANDQ
                     LNQVSKKWQAEVDALANVAKELYTKYQAEMVYLSDDMKKNKENEILAKEKELADLKRK
                     YFGSEGELYKKRESLLTPIQDEIYNAVKELAEEQSIMLILDRASGVNIVFASPAIDIS
                     DQVLKKMGY"
BASE COUNT         2384 a         1981 c         1887 g         2167 t
ORIGIN      
        1 ctgatccatg gtataagcct tggcgtatct cagatcgttt aggtaattct ccttgcgcag
       61 gctttcgagc aagcgtttgg ttgaagcctc gtccagcttg tattttccca gcttgatcaa
      121 aacatctccg gaacaacgct ccgctttgga gcaataatgc tgcgcccaat taagcgcctg
      181 cgaataatta tccatggcat ttagcggctt ttatcattcg gtcacggccg gaaatatcct
      241 gccgtagaat tatctcatca aaaccggcct cttcgaatag ctgcacaatc tccctgccgt
      301 aataaatgct gatttccaca tacaaacttc ccccgggttt cagggttcgg gcggccaagg
      361 ctgctagctc tctgtaaaag accagtgcat cgttgtcggg cacaaacata gccaaagccg
      421 gttcatgttc gagcaccctt ttttccattt tgagggcttc ggccgtggtc acatagggag
      481 gattgctcac aataacatca tacaccgaag cccggtctcc ggaattaaaa cggaacaaat
      541 cctgttcaat aaacgccaca tccagcccga gatctgcagc gttttcacgg gccaaagcca
      601 aagcatcggg cgatatatcc caggcttcaa cctgcatacc gggcaagtgt ttcgccaatg
      661 ccagcgatat gcaggcactg ccggtaccca cgtccaaaat cctgcccgtt ttgcccttgt
      721 tatccttgat tatccattcc accaactcct cggtttcagg tcggggaatc agaaccgatg
      781 gattgacact gagtttaaga tcgtagaaat aggcaactcc cgttatgtac tgcaaaggct
      841 cgccttctgc cagacgtccc agtttttcgt gcaactgccg cctttgtttg gttgaaattt
      901 cagtatcttt gccggcataa atatcgtttt tcgaaaagcc acagcaatcc tccagcaaag
      961 catacgacaa ctgcctgatt tcttcgggcc ggtattgttc tttcaggctt tgagccgcat
     1021 agctgaaaaa tgtttgcatg gttaacaatt tgtttacaaa aataatgcat ttctccttgc
     1081 tccacaacac aagtgccgac aaaaatctcg aggttctcta catgcgacgt tgcctgcaac
     1141 tggcagccaa cggacgcatc agttgttctc ccaatcccat ggtcggagcc ttgatagtac
     1201 atcaaaacac catcatcggc gaaggctggc atcacagtgc cggcaaggca catgcagaac
     1261 cccgagccat aaagtcggta aaaaaccggg agttgctcaa ggaatcaaca ctctacgtca
     1321 accttgaacc ctgctcgcat ttcggaaaaa ctccgccctg caccgatctg atcatcgaaa
     1381 aaaaaattcc ccgagtagtc attgcctgtc cggatccttt ccccggagtt tgcggaaagg
     1441 gcatagccaa attaaaagcc tccggcatcg aagtggaact gggtctggag gcagaggccg
     1501 ccaactacct gaacaaaagt tttttctgct attatcaaaa gcaacgtccc tatattgtcc
     1561 tcaaatgggc tcagacggcc gatgcttatt cagattacat tcgtcaaccg ggagacggac
     1621 aaaaaccctt acaaatatca agcgcctaca gccggatgct ggttcataaa ctcagggcag
     1681 aatccgacgc cattatgatc ggctctcgta ctgcgttgct ggacaatccc cgtctgacgc
     1741 tgaggcactg gcccggacga catcccttgc ggatagtaat ggatcgcaaa aacaccctgc
     1801 ccaaagactc gctgttgctg aacgatgaat ttcccagcct gatttttacg gaaaactttt
     1861 cggatcgccg cggcaacaaa gaatggctgg ctctgtccgg acacaactcc ggcagtatgc
     1921 ttggggatat gctggccgta ttgcatgcac gcaatattcg cagtctgttg gttgagggag
     1981 gcagcaccct gcaaaaagcc tttttggatg ccggtctttg ggatgaaatt cagacagaaa
     2041 cctggcatca taccataaaa aacggacata gggccctgcg cattcccgaa gatcagttga
     2101 aagcctgtag cattacaagt acaagtattg catccgaaaa caggaccata acgcattatc
     2161 tccgcaaaca ctgagcagaa aggctgttat cagactaatt attcataaaa aaacagttat
     2221 ttgtatttaa agctgaaatt cttcttactt ttgcgcttaa tttttggagc tgaatgaaca
     2281 aactactata tagtatcggg aaaaaagtct gcttgctggt attgctcagc cctgtactgc
     2341 tgggagtttc ctcttgccat agttttgagg atcaatcgtt ttccctggct aatgtgcaac
     2401 atttcgggtt tgattcggtg ccgggaatta cccgctacgt tttttacgtc gacaattttg
     2461 ccgggctcat ttacaataat gattcgctgc catatcaaac acggattgat tcgttggttc
     2521 ccagcatcgt atttttttcc tccaacggta atgtgtttat caacgacact atgctctgga
     2581 agccaggcga cgtattagat ttcagccgtc ccttatccct gacaaataca tccgacgacg
     2641 gcaaacacca aaagaaatac cggattaggg taaatactca tcagatcaat cccgattcca
     2701 tggtaatgaa tgtcctgagc agccggttcc caactattgc cgcccgaaat aaaaccattt
     2761 tatacaatgg cttgttcaag gctttttttg ctcccgatac gggctctata atcagctata
     2821 cttcgaccga tcaatgcctt acctggtcgg cagatgcccc ggtatccggg ctgaacgaca
     2881 ccattttcat tcagagcctc actgaattca agggacaata ttacctcctt ggaaagcaag
     2941 ggaggctgta ttattccggt gatttgaatc aatggtccgg atctgcagac agcaccaaag
     3001 ttgtaagcct ctttgctaaa ttggaaggcc gcaaatttct cagcgaagat gccttggttg
     3061 gaatcgtagc agacaacaac ggagatttac actttgcccg ctttgaggga gagtctgagg
     3121 gttgggaatg cggcactgcc gttcccgaaa atttccccct ctgcgagtat gcccttacca
     3181 aatacaaaac agtaaccgat gttccctata tcagcctgct ggggggtaaa aacgcctcag
     3241 gcgccctgaa tggagacagt tggagtacca tggacggcct gtattggatc aaagtcaaca
     3301 ctaaacccct gcatatcgaa cccagacaag gtgcttcctt atttcattac gacgataaaa
     3361 tgttttatta cgggggactc gacagccggg ggctttgcga caatactttg tatgtttccg
     3421 ggaataacgg tgtgacctgg tcggcagccg ataccctgct tcaattttac agcatagacg
     3481 gaggattggc ttaccaggat atttttgtag aagaaggcaa gtatatatgg attttcggcg
     3541 gagaatccgg cagcaacatc tcaaataaag tgtggaaagg caacattcac agaaaattat
     3601 ttcccgggaa ataagcgtat ctgcaaaatg aattcggctt ttttgcgtta gaataggaga
     3661 tgtaacagca acaaatcagc atgacaggac gaagagcatt cttttcgatt tgtttattac
     3721 tctgttcagc agcaatgtac ggacagagtt atcgcataga aatcggcggt ctgggaggcc
     3781 tgtcgtttta cacgggcgat gccaaccaga ctaagctttt cgagaaagtg catcctgctt
     3841 acggggtatt aatgcgctat cacctgaacc ggcgtttttc cataaaaggg aatctcctga
     3901 gtgcggggat atcgggcaca accacggaca agttggagca atatccggga gccgaagccc
     3961 tggatttttc ccgaagactc ttggatggag gcctgcagtt tgaattcaat ttttatgaat
     4021 tcggggcggc agactactac cccggggccg gtaatgtttc cccctacgta ttgtgcggaa
     4081 taggactaac cggatataaa accgataaaa acgcccttac cgccaatatt cctttgggat
     4141 tgggcattaa gctgaaatta cctgcccgtt tcaatctggg gctggaatgc acttatcgca
     4201 tgagcttttc cgataagctg gattacacgc ctcaatcaaa tttgtttcat ttggatgatc
     4261 cctggattgc tcaaagtgca tggaacaaaa ataaggacgg ctattttgtc ctggcttttt
     4321 acctaagtta tgacctgttt tatataggat cgaaatgtta taaataagat gtccggatta
     4381 gaaaaaatag acaaatcaac tctgcccaaa catgttgcca tcgtaatgga cggcaacgga
     4441 cgttgggcca aaagccgtgg caaagaccgc attttcggcc ataccgaagg gattaattct
     4501 gttcgtgccg ttgtggaagc ggctcgtgag ttgggacttt cttatttaac cttatacgtt
     4561 ttttcgacgg aaaactggaa tcgtcctcaa aaagaagtca acgcattgat gcggcttatg
     4621 acggaaacca tcgaccgcga aaccgacgat ttaatcaaaa atggcgtacg gggattggtg
     4681 gtaggagatt tagacagatt agaaaaagac attcaggata aagttcagca atttatgcat
     4741 aatacggctc agggtaatga actgaattta gtactcgctt tgagttattc ctcccgctgg
     4801 gaaattatgg aagcggtcaa aagcatcgcc aaaagcctat ccgagggaag aattctcgaa
     4861 caggagctgg atgaagattg tttttcgaaa catctttgca ccgccggcat tcccgacccg
     4921 gatttattaa tccgcaccgg gggcgaaatt cgtatcagta actttatgct gtggcaggct
     4981 gcttatgccg aattgtattt taccgaaacg gcctggcctg acttcaggaa agaagctttt
     5041 tatgaagcca tcatcgaata tcagtccaga gaaagacgct ttgggaaaac aagtgaacaa
     5101 ctaacaaaat aggacattga acaagactca ctcaataata tacaggctac ttgccggctt
     5161 tttatgtgtt ttttgcgcct tacctccgct tattgcacaa gaagaggcct tgccggagat
     5221 atattattcc tatcccagag aattgaaaat agccgacatt gccgtcgaag gtgaaggtat
     5281 agataattac gatgattatg tgctgattgg cctatccgga ttgtctgtcg gccaaataat
     5341 acgagtcccc ggaagcgaaa ccagcgaagc cgtaaaacgc ttttggaggc acggcctttt
     5401 ttcggatgtc aaaataatag cggacaaaat agaaggcaac gaaatatggc tccgcattgt
     5461 tctcagcccc cggccccgaa tttcgaaaat caattacaat gggatgaaga aatccgagat
     5521 tgaggaactt gaaccccgcc tgaatatgat tgtgggcaat cagattaccc ccaatatcat
     5581 cgacagagca gaaatactga tcaaaaaaca ctacgacggc aaaggctaca aaaatgccga
     5641 tgtcaatgta ctgcaacgcg acgatcctga atccgaggat caagtcatca ttgatatcaa
     5701 tgttgataaa caagataaag tcaaggttaa tcagttgatt atcgaaggta acgaggcttt
     5761 tcccgatgga aaagtcaagt ggaccatgaa aaagaccaac gaaaaaaaca agctgatcaa
     5821 ctttttcaga accaaaaaat ttgtcgatac cgaatacctg aacgacaaaa tcgccttgat
     5881 cgaaaaatac aacgaatacg gataccgcga tgccgtcatt gtagaagata ccgtttaccg
     5941 cttcgacgac aatacggtga acgtctacct gaaagtcgac gaaggacgtc aatactttat
     6001 ccgcgatata agctgggttg gcaacacttt atattcttcc gaagagttga acatgaggct
     6061 ccagatagaa aaaggcgatg tctacaatca aaaaagatta aacgaacgct tacatcagtc
     6121 gcccaacgaa gacgccgtaa ccgacctcta tatggacaga ggctacctct tttttcaggc
     6181 catacctgtg gaggtgtctg tggctgaaga ctccattgat ttggaaatca ggctcagcga
     6241 aggccagccg gccagcataa agcggataaa cataaacggc aacgaccggc tctatgaaca
     6301 cgtcatccgg agagaactct acaccaaacc cggagagtta ttcaacaaag ccgccttaat
     6361 gcgttcggcc cgtgaaatcg cacaaacagg gcatttcgat ccggaaacca tgggcatcaa
     6421 ccccattccc aacgaagacg gtacggtaga catcgatttg aaccttacct ctaaatcgaa
     6481 cgaccaggtg gaattttctc tcggctgggg aagcacaggt atagttggat cgatcggtct
     6541 gaaatttacc aacttttcca tgcgaaattt gctaaggctg ggtgaaggag ttttgcctca
     6601 aggagacgga caaaccttca gtattaccgg ccgcaccaat gggcaatatt accaatcgta
     6661 cagtttttcg tttatggaac cctggctggg cgggaaaaaa cccaactcct tttctttctc
     6721 gggttattat tcttatcaaa caggcgtaag caacaggtat tacgaaaatt actacagcaa
     6781 ttacatgatg cattatgcca cttacggcaa tacttacgat tacggcgatt attacctgac
     6841 cgaaatggac cccaccaaat acataaaaat gtggggctta tctgccggtt tgggtaccag
     6901 gttaacctgg ccggacgatt attttcatct gttctctgag ttttcttatc agcactacaa
     6961 cctggaaaat tggatgtttt tcctcatttc aaacggccgg agcaacgacc tgagccttaa
     7021 ctttaccctg agcagaaact ccacggacaa tccattgtac acccgctcag gttcttctta
     7081 ttccttatcg ctggggatca ctcctccgtt ttctttgctc gacaacaaaa ccgacgaaga
     7141 ttacaaaaaa atgagcagtc aggaactcta caactggata gaatactata agattcgttt
     7201 ccgctccaga acctataccc cactgagcgg gaaccggaaa ttggttatgg ccacccgctt
     7261 cgatattggt ttgctcggcg cctttaatca atacaaaaga tcgcctttcg gtacgttcta
     7321 tatgggaggc gacggtatga caggttactc cagcagctat gcctacgata tcatcgcgct
     7381 ccggggttac ggcaacggcg atctgggtgt tggctggctc tatcaaaggg ccggcttgga
     7441 actgcgctat cccttgttga tggaacaatc ggctaccatt tacgcttctg cgtttttgga
     7501 agcaggcaac ctctggtctg atttcaagca ttattctccc tttgaattga aacgttcggc
     7561 gggcgtaggc gtgcgcatat ttatcccgat agtagggtta ttgggaattg actgggctta
     7621 cggatttgat ccggtcggta ccgaaaccaa agcccacggc ggtgaatttc atttcgtcat
     7681 aggacaagaa ttttaaaatt tgtttttaac tttgaccggc ttttaagtca aatacaaaaa
     7741 tctcaatggt atgaaaaaag cttatttatt aataatcagt ttgattttta gcagcatggc
     7801 cgtatttgct caaaaatacg ccttggtcga tatggaatac attttttcgc gtatccccgc
     7861 ttacgaaatg gccaacgacc aactgaacca ggtgtctaaa aaatggcagg ccgaagtaga
     7921 tgcccttgct aacgtggcca aagagctata cacaaaatat caggccgaaa tggtttatct
     7981 ttcggacgat atgaaaaaga acaaagagaa cgaaatcctg gccaaagaaa aagagctggc
     8041 cgatttgaag cggaaatatt tcggttccga aggcgaactc tataaaaagc gcgaaagcct
     8101 gcttacccct attcaggacg aaatctacaa cgctgtcaag gaactggccg aagaacaatc
     8161 cattatgtta atactggacc gggcctccgg ggtcaatatt gtttttgcct ctccggcgat
     8221 agacatcagc gaccaggttt taaagaaaat gggttattaa tccctatcag ccttttatta
     8281 gttggataac actttttatt atcaatacaa aaattacgac gttatgtata aacatttcat
     8341 tgttgctgtc ttatgttttt tgcccctgct ggcagttgct caggaattcg gttacgtcaa
     8401 cagagccgaa atatttcag
//
LOCUS       DAOSGL010000064         8257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7414_length_8257_cov_4.490246, whole genome shotgun sequence.
ACCESSION   DAOSGL010000064 DAOSGL010000000
VERSION     DAOSGL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7414_length_8257_cov_4.490246"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PKY68_03285"
     CDS             complement(<1..690)
                     /locus_tag="PKY68_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOH24092.1"
                     /translation="MTISTELILLVGSVLFFISMLVGQAGHRFGVPILLLFLMVGMIF
                     GSDGFGLKFENIQMAQAIGSVCLAIILFSGGLDTRISEIKPVIGPGVVLATLGVLMTA
                     VFTGLFTYWMTDNFFPALGLGFLSCMLLASTVSSTDSASVFAILRSKGLTLKNNLRPL
                     LELESGSNDPMAYLLTVMFIDIIQYGENPDYLRVGLSVLIQLVVGTAMGILLGRLAVK
                     IINKVKMDNDSF"
     gene            complement(961..1779)
                     /locus_tag="PKY68_03290"
     CDS             complement(961..1779)
                     /locus_tag="PKY68_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24093.1"
                     /translation="MTKTKVLITVKTYPTISKKYEELVCTAGFLEDGSWIRIYPIQFR
                     KKSYSEQYSKYDWIELDLVKNKSDFRPESFRPISHDTVIEKVGEIEADGDTWDERRKI
                     VLRNVYHNISDLISEAKNKKTCTSLAVFKPTEVLDFIYEEEKVRVWSSDKLAQFQQLN
                     IFEKVKSNNFQVVRKLPYKFSYCFKDEAGTVCKLMIEDWETGQLYWNCLSRHDGDEKK
                     ACEDVRKKYFDDFVKTKDLHFFLGTTQVHHFRAKNPFVIIGTFHPKPITQLKLF"
     gene            complement(1779..2663)
                     /locus_tag="PKY68_03295"
     CDS             complement(1779..2663)
                     /locus_tag="PKY68_03295"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOH24094.1"
                     /translation="MYYRRKILLSLLQAFDNQLDKISLQKLLFLLTRIQEKKSYYFIP
                     YKFGCFSFQANADLSTLAKYKLVEPSMKYWKKTDNTDYIEELDSKDKAAIRFLKSQYG
                     NLSKDELIALTYRQYPFFAINSIIASSILTNEELNKVKNQRPIKNTIVLFTIGYEGIS
                     LEQYINKLIVNDIRVLCDVRKNSYSMKYGFSKNQLKKACGGVGIDYVHIPEVGIETKK
                     REVLNSQSDYDKLFEYYKSDVLAREKSKQVEIFSLLKSKRRIALTCFEANINQCHRKH
                     LAESISSLNGFSYELKHI"
     gene            complement(2860..3390)
                     /locus_tag="PKY68_03300"
     CDS             complement(2860..3390)
                     /locus_tag="PKY68_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOH24095.1"
                     /translation="MIKLIRGDITKIKVDAIVNAANTSLLGGGGVDGAIHRAGGKTIL
                     EQCIKIREKQGGCKVGEAVLTGAGELPAKYVIHTVGPIWRGGNQNEDRLLSNAYRNSL
                     QLAAENKVESISFPNISTGVYHFPKERAANIALTTVMDYLKSNPAIKEVIFVCFDQEN
                     YDIYDRLLNGTEGKLN"
     gene            complement(3437..4441)
                     /locus_tag="PKY68_03305"
     CDS             complement(3437..4441)
                     /locus_tag="PKY68_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOH24096.1"
                     /translation="MAIRNKKTNNKNLKLRVQRIIAIFSVLIFAGKLTAYFLTNSVGI
                     LTDALESTVNVITGFITLYAVYLSLKPKDENHPFGHGKAEFLSASIEGFLITIAGLVI
                     IFEAIKRLFIPVQITQLDIGIIIVAVAGLLNYLIGWYSIRIGKQANSIALISGGKHLH
                     SDTYSSIGLVIGLVLLYYTKLAWLDSAIGLIFGTVIVITGLKILRETTSNLMDKADFK
                     LIEQFGKIIEDNKPDEWIDIHNFKLIKYGNAYHINCDLVLPWNLYLSEAHEEGEKLKN
                     LLVSNFREDIVFNVHTDECFKKYCKHCKRKDCKERTTGFVTRLNFDLERFTKEKIEKS
                     "
     gene            complement(4525..4857)
                     /locus_tag="PKY68_03310"
     CDS             complement(4525..4857)
                     /locus_tag="PKY68_03310"
                     /inference="COORDINATES: protein motif:HMM:NF037850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24097.1"
                     /translation="MLVLSADRNEIIADGQDLSYIRVEITDKNGVIAANASNKLEFEI
                     EGPGVIIAVDNANLKDTNSYVSNTRNAWKGKALVIVGSTRDSGSISLKVKSPGLKESG
                     ITIQTMEN"
     gene            complement(4835..>6489)
                     /locus_tag="PKY68_03315"
     CDS             complement(4835..>6489)
                     /locus_tag="PKY68_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH24098.1"
                     /translation="VNAKRALVEISTEIKNDTDKTQHIILSTKIKSENGGAKDQVAED
                     KIWSNPPNVLRQWEAKDQVAEGQMGKNPMVKTQMVKGRVAKAQTTETQISIEPGVEKQ
                     IVQRIRVKRPLLWSPESPNLYRADQEVLCENRVTDKTTHTFGIRTIEFSSTDGFLLNG
                     EPVILNGGCVHSDNGSLGAAAYNRAEERRVELLKSAGFNAVRTAHNPPSEAFLDACDR
                     LGLLVIDEAFDGWKAPKTPYDYSRHFDEWAKRDMQDLVLRDRNHPSVIMWSIGNEVIE
                     RTEPEAVETARMLASSVKEIDSTRPVISAMTTWGQGWEIFDPLMAEHDICGYNYQLWR
                     APDDHKRVPSRIIVNTESYPGDAFSIWKLVNENNYILGDFVWTAIDYLGEAGIGAYYY
                     PGEDPAEHWQSSRYPWHGAYCGDIDLLGWRKPISHYRSMLYNDNEKLYLAVREPNPEG
                     GEIKLTGWAVWPTWESWTWPGHEGKNIEVEVYSKYPAVRLYLNDVLIGEKQTGETEEF
                     KALFTLPYTPGLIKAVGLENGKETETKVLQTRRSRNACIIRR"
     assembly_gap    6490..6589
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6590..7242)
                     /locus_tag="PKY68_03320"
     CDS             complement(<6590..7242)
                     /locus_tag="PKY68_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOH24099.1"
                     /translation="MTSYFKTIILAVTILLVSCSGANRPDENIERKHLFNFDWKFHLG
                     DVQDASSESFNDGDWRILDLPHDWSIEATFEKHHPAGNDGGYLPTGVAWYRKTFKAPF
                     QWKDKKTALYFEGVYENSEVFINGISLGVRPYGYSSFLYDLTPHLKAGEENVISVRVD
                     NSNQKNCRWYTGSGIYRHVWLIVTDKIHIKHWGVGISTPRVNAKRALVEISTEIKNDT
                     "
     gene            complement(7298..>8257)
                     /locus_tag="PKY68_03325"
     CDS             complement(7298..>8257)
                     /locus_tag="PKY68_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOH24100.1"
                     /translation="YGGPNPTSHRAILERASDMVSKLADGKHIFYCDVNHVFLNMDGS
                     INREAMPDWLHPSPAAAKAWAQAMEPLLCELMGDESRDTEIPANTALVPAPNLEDNSY
                     DWLGRHKEILSIKDSIDPEIVLIGNSITHLWGGEPPMRWADGKLREHNGPKSWDSLFH
                     DYRVLNLGFGWDRTQNVLWRLDRGELDGLHPRTVIINIGTNNTGQSQHGRMNTAPEIV
                     EGIRAICLRVRSKVPDARIVLMAVFPREQTKDHPRRILINEINAGLESFAREQKITFL
                     NIGSKMLDADGSFLPGLTFDFCHPTEKGYQLWADEIRPIISEP"
BASE COUNT         2190 a         1810 c         1565 g         2592 t
ORIGIN      
        1 aaacgaatcg ttatccattt taactttgtt gataatttta accgctaaac gccctagcag
       61 tattcccatt gcagtaccca caaccaactg gatcaacaca ctaagtccca ccctgaggta
      121 gtcgggattt tcgccgtatt gaataatatc gataaacatc accgtcagca aataggccat
      181 cgggtcgttg ctaccgctct ccagttcaag taaaggacgc aggttgtttt tcaaggttaa
      241 gcctttcgaa cgcaaaatgg caaaaacgga agccgaatcg gtcgacgaaa ccgtcgaagc
      301 gagcagcata cacgacagaa atcctaaacc taatgcgggg aaaaaattat ctgtcatcca
      361 ataggtaaac aagcctgtaa aaacggccgt catcaacact cccagggtag caagtactac
      421 tccggggccg ataacgggtt ttatttccga aattcgggta tccaaaccac ccgaaaacag
      481 aataatggcc aaacaaaccg agcctatggc ttgtgccatt tgtatatttt caaatttaag
      541 cccgaaccca tcggaaccaa agatcatgcc caccataagg aaaagcaata aaataggcac
      601 cccaaagcgg tggcccgcct gccctaccag catactgata aaaaacagta ccgagccgac
      661 aagcaatatt agttctgttg aaatagtcat ctgtaattat tcaaatttaa ctgaatcctc
      721 ttattgcatt tattggcgca cgtgctccca cactaacgtc aaaattttta tttgcagggc
      781 aaagatacaa catttggaaa taaaaaatat agggtgcgtc cctgataacg atcatgcaca
      841 cagataagat aagcacctat gccgtttggg acgaactgga aggctgggca aaattggatt
      901 gcagcgtggc ttgtgcgttt tgccaaagtg cctgacacga ggagaagatt ttgaattgac
      961 ttagaatagc tttaattgag ttattggttt tgggtggaat gttccaataa ttacaaatgg
     1021 atttttagct ctaaaatgat gaacttgagt tgtacctaaa aagaaatgta agtctttagt
     1081 tttaacaaaa tcatcgaaat attttttcct tacgtcttca catgcctttt tctcatctcc
     1141 atcatgtcga cttagacaat tccaataaag ttgtccagtc tcccagtctt caatcataag
     1201 tttacaaaca gtacctgctt catccttaaa acaataactg aatttataag gtaatttcct
     1261 aacaacctga aagttgttgc ttttaacttt ctcaaatata tttaactgtt gaaattgtgc
     1321 caatttatct gatgaccaaa cccttacttt ttcctcttca taaataaagt ctaaaacttc
     1381 tgttggcttg aatactgcca atgaagtgca agtctttttg ttttttgctt ccgaaattag
     1441 gtcggaaatg ttatgataga cgtttcttaa aactattttt cttctttcgt cccaagtatc
     1501 accatctgct tcgatttcgc cgaccttctc tatcacagta tcatgagaaa tagggcgaaa
     1561 actttcggga cggaaatcgc ttttattttt gactaaatcc aattcaatcc agtcatattt
     1621 agagtattgt tcactgtacg attttttacg aaattgaata ggataaattc taatccatga
     1681 accatcttct aaaaatcctg cagtgcaaac taattcttca tacttttttg agattgtagg
     1741 ataggttttt acggtaataa gaacttttgt tttggtcatc agatatgttt tagttcatag
     1801 gaaaaaccat ttaaagaaga tatcgattcg gctaaatgtt ttctatggca ttggttaatg
     1861 ttagcctcaa aacaagtgag tgctattcgt cttttacttt ttaacaacga aaaaatttcc
     1921 acctgctttg atttttctct tgctagaacg tcagatttgt aatattcaaa aagtttgtca
     1981 taatcagact ggctgtttag tacttctctt ttttttgttt caatcccaac ctcagggata
     2041 tgcacataat caattccaac acctccacaa gctttcttca gctggttctt actaaaaccg
     2101 tatttcatgc tgtacgaatt ttttcgcaca tcacacaaaa cccttatatc gttaacaatt
     2161 aacttgttaa tgtactgttc aagcgaaatt ccttcataac caattgtgaa taaaacgata
     2221 gtatttttta tcggcctttg gttttttact ttgtttaatt cctcgttggt cagaatgcta
     2281 ctcgcaatga tactattaat tgcaaaaaac ggatattgac gataggttaa agcgattagt
     2341 tcgtctttac tcaaatttcc atactgactt tttaaaaatc gaattgcagc tttatcttta
     2401 gaatctagtt cttctatgta gtctgtatta tctgtttttt tccaatattt cattgatggt
     2461 tctaccaatt tgtatttagc caaagtactt aaatcggcat tggcttggaa tgagaaacat
     2521 ccaaatttat acggaataaa atagtacgat ttcttctcct gtatccttgt tagtaagaat
     2581 aacagttttt gcagactaat tttatctaat tgattatcaa aagcctgaag cagggatagt
     2641 aatatttttc tacgataata cataaggcaa agctacaata taaaatggtt tcttccatca
     2701 taattgcttc tttctctttg ctaaaaattt cgctcgactg ctactaacac tatgtatttg
     2761 tttgtcaaat aataacatac agttaaataa tcgggaacag tgggggcttc attagtcttt
     2821 tcagtcaata tgcagcaaag gtgtcttaac ggctggtgtt caattcaatt tcccctctgt
     2881 tccattcaat aacctgtcgt aaatatcgta attctcctgg tcaaagcata caaaaatcac
     2941 ctctttgatg gcagggtttg attttaaata atccataacg gtggtcagtg caatgttggc
     3001 tgctctctct tttgggaaat gatagactcc tgtgctgatg ttgggaaagg aaatcgattc
     3061 tactttgttc tctgccgcaa gttgcaagct gttccggtag gcattcgaca aaagccggtc
     3121 ttcgttttga ttcccgccgc gccaaatagg tccgacggtg tgaattacat attttgcagg
     3181 caattctcct gccccggtca gcacggcctc cccaactttg cagccgccct gcttttctct
     3241 gattttaatg cattgttcga gaattgtttt accgcctgct ctgtgaatgg ccccatcaac
     3301 gccaccacca ccgagcaatg aggtgtttgc ggcattcaca atggcgtcga cttttatttt
     3361 ggtgatgtca ccacgaatta attttatcat tacttgttgt tttaaaatgt attgttctct
     3421 cgaatagctt ccaagcctaa cttttctcta ttttttcttt tgtaaatctt tccagatcaa
     3481 agttcaatcg agttacaaag ccggtggttc tttccttgca atcttttctt ttacaatgct
     3541 tgcagtattt cttaaaacat tcatccgtgt gcacgttaaa aacaatatct tcccggaaat
     3601 tggagaccaa taaattcttt agtttttccc cttcttcgtg agcctccgat aaatataaat
     3661 tccaaggtaa aaccagatcg caatttatat gatatgcgtt cccgtatttg atgagtttaa
     3721 aattgtgtat atctatccat tcatcgggtt tgttatcttc aattattttc ccgaattgct
     3781 caatcaattt aaaatcggct ttatccatca gattggatgt ggtttccctg aggattttta
     3841 agcccgtgat cacaattacg gttccgaaaa tcaggccaat tgcactgtct aaccaggcta
     3901 atttcgtgta gtacagcaac actagaccaa tcactaagcc aatggatgaa taggtgtcgg
     3961 agtgtaagtg tttccctcct gatattaaag caatggaatt ggcttgtttt ccgattctta
     4021 tgctgtacca tccaattaaa taattcaaga gccccgcaac ggcaacaata ataatcccta
     4081 tgtccaattg agtaatctgc accggaataa aaagtcgctt aatggcttcg aaaattatta
     4141 ctaacccggc aatagtaatt aaaaacccct cgatcgatgc ggataaaaac tccgctttcc
     4201 catgcccaaa cggatggttt tcatctttgg gttttaacga aagataaacg gcgtacaaag
     4261 taataaaacc ggttataacg tttaccgtac tttcgagggc atccgttaat atacctactg
     4321 aattggttag aaagtaagcg gttaattttc cggcaaaaat cagaaccgaa aaaattgcaa
     4381 taatgcgttg aaccctcaat ttcaggtttt tattatttgt ctttttgttt cttattgcca
     4441 taccggtttt ctataaacga ctgcttgctt tttctttgca atgaatgttt tttgcagtga
     4501 acgcttttcc cccgtaaatc tactctaatt ttccattgtt tgaatagtaa tgccggattc
     4561 tttcaaaccg ggagatttta ctttcaaact aattgatccg gaatccctgg tgcttccgac
     4621 aataaccaat gcttttccct tccatgcatt tctggtattg gatacgtatg aattagtatc
     4681 cttgaggttg gcattatcaa cggcaattat cactccgggg ccttctattt cgaattccag
     4741 tttgtttgag gcatttgccg caatcactcc attcttgtcg gtaatttcaa ctcttatgta
     4801 agaaaggtct tggccgtcgg caatgatttc atttctatct gcggataata caagcatttc
     4861 tgcttcgccg ggtttgcaac accttcgttt ctgtttcttt tccgttctcc aatccaacgg
     4921 ccttaattag gccgggagtg taaggaagag tgaatagggc tttgaattcc tctgtttctc
     4981 cggtttgttt ttctccaata agtacatcgt ttaaataaag gcggacagcc gggtatttcg
     5041 aatacacttc cacttcgatg ttttttcctt catgccccgg ccatgtccag ctttcccatg
     5101 tcggccaaac agcccatccg gttagtttga tctctccgcc ttcgggattg ggttctctta
     5161 cggccaggta tagtttttca ttgtcgttgt acaacatact tctgtaatgc gaaataggct
     5221 ttctccaccc caataagtca atatctccgc aataggctcc atgccagggg taacgactgc
     5281 tttgccaatg ttcggcggga tcttcaccgg gataataata agctcctata cctgcttcac
     5341 ccagataatc tatggcagtc caaacaaaat cgccaagaat gtaattgttc tcgtttacca
     5401 atttccaaat cgaaaaagca tctccgggat aggattccgt atttacgata atcctcgaag
     5461 gaactctttt gtggtcgtca ggagcacgcc aaagttggta gttatatccg caaatatcgt
     5521 gttcggccat cagcggatca aaaatttccc agccctgccc ccaggtggtc atggcagaga
     5581 ttacaggacg ggttgaatct atttccttca cgctcgatgc caacattctc gccgtttcta
     5641 cagcttcggg ttctgttctc tcgattactt cgttccctat actccacata attacggatg
     5701 gatggtttct gtctcgcaac actaaatcct gcatatcccg cttagcccat tcatcgaaat
     5761 gtcttgaata atcgtagggg gttttcggag ctttccagcc gtcgaaggct tcgtctatca
     5821 ccaaaagccc caacctgtcg caagcatcga gaaatgcttc cgagggagga ttgtgggctg
     5881 tgcgcacagc gttgaaaccg gccgatttca gtaattccac tctccgttct tctgcccgat
     5941 tgtacgcagc agcgcctaaa cttccattat cgctgtgaac acaacctccg tttaaaatca
     6001 ccggttctcc attaagtaaa aatccatcgg tactactgaa ttctatggtt cttattccga
     6061 atgtatgtgt tgttttgtcg gtgacccggt tttcacataa tacttcctgg tcggcccggt
     6121 atagatttgg agattccggt gaccatagca aaggtcgctt tactcttatt ctctgtacta
     6181 tttgtttttc aacacccggt tcaattgaga tctgagtttc ggtcgtttga gctttggcca
     6241 cccggccttt aaccatttga gttttgacca tgggattctt gcccatttga ccttcagcta
     6301 cttggtcttt agcctcccat tgccttagaa cgttaggcgg atttgaccaa attttatctt
     6361 cagctacttg gtctttagcc ccgccgtttt cgctttttat tttggttgaa aggattatat
     6421 gttgagtttt gtctgtatcg ttttttattt ctgtgcttat ttcaacaagg gctcttttgg
     6481 catttacccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tatcgttttt
     6601 tatttctgtg cttatttcaa caagggctct tttggcattt acccgcggag tgctgatacc
     6661 cactccccag tgttttatgt gaattttatc cgttactata agccaaacat gcctgtagat
     6721 acccgaaccg gtgtaccaac ggcagttttt ctgattggaa ttatccacgc ggacagaaat
     6781 aacattttcc tcacccgctt tgaggtgagg tgttaaatcg tagagaaaag aagaatatcc
     6841 gtatggccgc acacccaaag atatgccatt gataaacact tccgaattct cgtaaacccc
     6901 ttcgaaataa agggctgttt ttttgtcttt ccactgaaac ggtgctttaa acgttttgcg
     6961 ataccaggcc actccggtcg gtaaatagcc tccgtcgttc cctgcaggat gatgcttttc
     7021 gaaagttgct tcaatgctcc aatcgtgggg taaatcgagg attctccaat cgccgtcgtt
     7081 aaaactttcc gacgaagcgt cctgaacatc gcccaaatga aatttccagt cgaaattgaa
     7141 caagtgcttg cgttcaatat tttcatccgg ccgattagca ccggaacaac ttaccaacag
     7201 gatggttacg gcaagaataa tggttttaaa ataggaggtc atggtgggtt ttatttatag
     7261 ttatttatgc gaaatgaagc gcccgaaaac ctttgtatta aggttcagaa atgatcggac
     7321 gaatttcgtc ggcccatagt tgataacctt tttctgtggg atggcagaaa tcaaaagtca
     7381 ggccgggtag aaagcttccg tcggcatcga gcattttcga ccctatgttc aggaaagtga
     7441 ttttctgttc cctggcgaaa gattcaagtc ctgcgttgat ttcgtttatc aatatacgcc
     7501 gcggatgatc cttggtttgt tcgcgcggaa aaacggccat cagcacaatt cttgcatcgg
     7561 gaactttgga gcggacacgc aaacaaatgg ctcgtatgcc ctcgacaatt tcgggagccg
     7621 tattcattcg cccgtgttgg ctctgccccg tattattggt gccaatattt ataataactg
     7681 tgcgaggatg aagcccgtcg agctctcccc ggtcgagcct ccaaagaaca ttttgcgtgc
     7741 ggtcccagcc aaaaccgaga ttcaacactc ggtaatcatg aaacaacgaa tcccaggact
     7801 ttgggccgtt atgctcgcgc aatttcccat cggcccatct catcgggggc tctcctcccc
     7861 ataaatgagt aatggaattg ccgataagta caatttcggg atcgatggaa tctttgatac
     7921 taagaatttc tttgtgacgt cccaaccaat cgtagctgtt gtcttcgagg ttgggagcgg
     7981 gcacaagggc tgtattggca ggaatttccg tatcgcgcga ttcgtcgccc ataagctcgc
     8041 atagaagagg ctccatggcc tgagcccagg ctttggcggc agcagggctg ggatgaagcc
     8101 aatcgggcat cgcttcacgg ttgatggagc catccatatt aagaaacaca tggttcacat
     8161 cgcaatagaa aatatgcttc ccatcggcaa gcttcgacac catatccgaa gcccgttcga
     8221 ggattgcccg gtgagaggtc ggattgggtc cgccata
//
LOCUS       DAOSGL010000065         7982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7788_length_7982_cov_4.382238, whole genome shotgun sequence.
ACCESSION   DAOSGL010000065 DAOSGL010000000
VERSION     DAOSGL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7788_length_7982_cov_4.382238"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1948
                     /locus_tag="PKY68_03330"
     CDS             20..1948
                     /locus_tag="PKY68_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOH24101.1"
                     /translation="MVAKIPEGALSEKWSNYKAQQKLVNPSNKRRLDVIVVGTGLAGA
                     SAAAALGELGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKGG
                     DYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRQVHKGSVKLYTRREMLDLVIVDGKARGIIVRNLITGKIERYSAH
                     AVVIGTGGYGNAFFLSTNAMESNGSAAWQVYKKGAYFANPCYAQIHPTCIPVQGEMQS
                     KLTLMSESLRNDGRIWVPKKKEDAQAVRAGKLKPTEIAEEDRDYYLERRYPAFGNLVP
                     RDVASRAAKERCDAGYGVGSGLAVYLDFKDAIERLGKDVVETRYGNLFQMYEKIVDDN
                     PYETPMMIYPAIHYTMGGIWVDYELQTTIPGLYSIGEANFSDHGANRLGASALMQGLA
                     DGYFVLPYTIQNYLADEIRTPRISTDLPEFAEAERNILERIERLMSIKGKRSVDSLHK
                     QLGHIMWDFVGMGRNKKGLLKAIDEIKQVKKEFWSNVFIPGEGLDLNVELEKALRLAD
                     FIEIGELMARDALHREESCGGHFREEYQTPEGEALRQDDKFNYVACWKYMGEDKEPEM
                     YKEELKYEFVQVQQRNYK"
     gene            1950..2705
                     /locus_tag="PKY68_03335"
     CDS             1950..2705
                     /locus_tag="PKY68_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOH24102.1"
                     /translation="MEKTININLRVWRQRGPKAKGAFESYKLDGVSTDSSFLEMLDQL
                     NEQLISEGKEPIVFDHDCREGICGMCSLYINGHPHGPDEDITTCQLHMRKFKDGETIT
                     IEPWRSHGFPVIRDLMVDRSAFDKIIQAGGYVSVNTGGIPDANAIPIPKPVADMSMDA
                     AACIGCGACVAACKNGSAMLFVAAKVSQLALLPQGKVEAARRAKAMVQKMDELGFGNC
                     TNTGACEAECPKNISITHIARLNREFLKAKFKD"
     gene            2712..3500
                     /locus_tag="PKY68_03340"
     CDS             2712..3500
                     /locus_tag="PKY68_03340"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOH24103.1"
                     /translation="MAVQDYVRSESFDYPEAPDSLAADVTENSLTLQRPLYEGIPRQE
                     GPLFGLKPFIVLILGLFVFSYIFGPRSSLFLHSLKDIFVYRERSSIFVDSDVRLVRYR
                     RLLSVFGFLVCLLLLSLIFQPEQNASLNSLDARSLGYALLFLGVFVLAKFLLYRFFTS
                     FQFDVSTARRWQNDNLILFIVLSFSYAVLVFVNEIVLIGPECLLKIALILWILDKMIL
                     LAKTVHYFSDQRLSWFHFIIYLCALEIIPACIFYKGIFWVRGIV"
     gene            3518..4267
                     /locus_tag="PKY68_03345"
     CDS             3518..4267
                     /locus_tag="PKY68_03345"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOH24104.1"
                     /translation="MKIKKILVSQPQPATEKSPYFDIEKKYGVKFVFRPFIKVEGISA
                     KEFRQQKIAIQDYSAVVFTARTAIDHFFRLCEELRVSVSEDLKYFCIGEQIALYLQKY
                     IQYRKRKIFYPESGRVEDLVELIQKHSNDNNFLVSVSYVHKDDLISKLDENNINYTKA
                     VMYRTVSNDFEEGEAFDYDMLIFFSPSGIVAMLKNFPNFVQGDIHIACLGPTTARAIR
                     EAGLRLDLEAPTPNAPSITAALDQYLKDNGK"
     gene            4364..4756
                     /locus_tag="PKY68_03350"
     CDS             4364..4756
                     /locus_tag="PKY68_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOH24105.1"
                     /translation="MDKSQLSFGKAERLCGELRINKLFEQGKSFVVYPLRVCWLLMPP
                     AEKAPVQVMVGVPKKKLRKQVQRNRVRRLIRETYRLSKGDLLNEVRGKEAQLLLAFIW
                     MSEQVPEYKDLETKMQEALRQLRKKLSL"
     gene            4753..4974
                     /gene="yidD"
                     /locus_tag="PKY68_03355"
     CDS             4753..4974
                     /gene="yidD"
                     /locus_tag="PKY68_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOH24106.1"
                     /translation="MRKLAIFLVLLPVRFYRLFISPLIPSSCRYTPTCSEYALQAVQK
                     YGPVKGVRLAIRRILRCHPWGGHGYDPVP"
     gene            4995..5681
                     /locus_tag="PKY68_03360"
     CDS             4995..5681
                     /locus_tag="PKY68_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOH24107.1"
                     /translation="MFIDIHTHREQHYLKFIEQEQFDSLSPRWDNSRWFFLNIESEDF
                     IPGSNLPPFLSLSTGIHPWKADVLPDTEKLEAVFAHPDIRLIGEIGLDKRCTVPYELQ
                     FQVFELQLEMAGTLKKPVLIHQVSSMDKILVCKKAHPEIPAWIIHGFRGKKTQALQWL
                     QHDFFLSFGPQFNNAALQACPPERLFFETDASGIAIEEIYKKAAQVFACPVAALELQV
                     ERNVNSLFNQ"
     gene            complement(5671..6354)
                     /locus_tag="PKY68_03365"
     CDS             complement(5671..6354)
                     /locus_tag="PKY68_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOH24108.1"
                     /translation="MKTLRNSALIFATKPSAITPQIEEFLKDRKAYFETTNNVQKALD
                     FITTDSPCLVIFDTPTDDPKALDLCKKLKKLKEESRFNIYFLIDNNSNLEEQLNWFKN
                     GVDEFGTRDTNSILICQRIRNILAKYEDSLIDVIPQKGIVIDSDRYLVYKNGKEIFLP
                     RKEFELLQLLNSRPTKVFTRDEIFRHVWGNRSIVGDRTIDVHVRKIREKIGSNHIVTV
                     KGVGYKFIG"
     gene            6559..7860
                     /gene="tyrS"
                     /locus_tag="PKY68_03370"
     CDS             6559..7860
                     /gene="tyrS"
                     /locus_tag="PKY68_03370"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOH24109.1"
                     /translation="MNFIEELSWRGMIHDMMPGTEEQLKKEMTTAYVGIDPTADSLHI
                     GHLVGIMMLRHLQRSGHKPIALIGGATGMIGDPSLKSGERNLLDEETLRHNQEGIGRQ
                     LAKLLDFESTAPNAAELVNNYDWMKDYSFLNFIRDIGKHITVNYMMAKDSVKKRLSAD
                     SNVGMSFTEFSYQLLQGYDFLYLNEHKNCKLQMGGSDQWGNITTGTELIRRKNGGEAY
                     ALTCPLITKADGGKFGKTESGNIWLDPRYTSPYKFVQFWLNVSDADAEKYIKIFTFLG
                     REEIENLCVQHNKAPHLRLLQKRLAEEVTCMVHSREEYEAATEAAAILFGSSSTEQLL
                     RLDEKTFLDLFEGIPVFQVDLDLFKQGVKAVDLLAEHAAVFPSKGEMRKMVQNGGLSI
                     NKNRCQQFDQLLDTSFLLHGRYLLIQKGKKNYFLITASSEN"
BASE COUNT         2200 a         1674 c         1876 g         2232 t
ORIGIN      
        1 cgataaaaaa actaagacaa ttgtcgcaaa gatcccggaa ggtgcgttga gtgaaaaatg
       61 gtcaaattac aaggctcaac agaagctggt aaatccctcc aacaagcgtc gtctggatgt
      121 aattgtggtg ggcacaggtt tggcgggagc ttccgctgca gccgcacttg gtgagttggg
      181 cttcaatgtg ctgaatttct gtattcagga ttctccccgt cgtgcacact ccatcgccgc
      241 acagggaggt atcaatgccg ctaaaaatta tcaaaacgac ggtgattcgg tttatcgttt
      301 gttttacgat accataaaag gaggagacta tcgcgcccgc gaagccaacg tttaccgcct
      361 ggccgaggtt tccaacagta tcattgacca atgcgtagcc cagggcgttc cttttgccag
      421 ggagtacggt ggtttgctcg acaatcgttc ctttggcgga gcccaggtct cccgtacttt
      481 ttatgccagg ggacaaacgg gccagcaact gttgcttgga gcttattcgg ccttgagccg
      541 tcaggttcac aagggcagcg ttaagctcta tacccggcgg gaaatgctcg atctggtgat
      601 tgtggacggc aaagccaggg gcatcattgt ccgcaacctg ataaccggaa agatcgagcg
      661 atattcggct catgccgtgg taatcggtac aggaggctac ggcaatgctt ttttcctctc
      721 gaccaatgcc atggaatcca atggttcggc tgcctggcag gtgtacaaaa aaggtgctta
      781 ttttgccaat ccctgttacg cacagattca cccgacctgc attcccgttc agggagaaat
      841 gcagtccaaa cttacactga tgtccgaatc cctacgtaac gacggccgca tctgggtgcc
      901 caagaagaag gaagacgctc aggctgtccg tgcaggcaaa ttaaaaccaa ccgaaatagc
      961 cgaagaagac agagattatt acctggaacg caggtatccg gctttcggca acctggtacc
     1021 cagagacgta gcctcgagag cagccaaaga acgttgcgat gccggatacg gagttggaag
     1081 cgggctggcc gtatatctcg atttcaaaga cgccatagag cgtttgggta aagatgttgt
     1141 tgagactcgt tacggcaacc ttttccagat gtacgaaaaa atcgtggacg acaatcctta
     1201 cgaaacgccc atgatgattt atccggccat tcactatacc atgggaggaa tctgggttga
     1261 ttacgaattg cagactacta ttcccgggct ttacagtatt ggagaagcta acttttccga
     1321 tcacggagca aaccgcctcg gcgcgtcggc tttgatgcaa ggtttggccg acggctattt
     1381 tgtattaccc tataccatac agaattacct ggcggacgaa atacgcacac cgcgtatttc
     1441 tacggatttg ccggaatttg ccgaggcaga acgaaacatt ctggaacgta tagaacgact
     1501 tatgtcgatc aagggaaaac gttcggtaga ttccctgcat aagcaattgg ggcacattat
     1561 gtgggatttt gtggggatgg gccgcaacaa aaaaggtttg ctcaaagcca ttgacgagat
     1621 caaacaagtt aaaaaggaat tttggagcaa tgtctttatc ccgggcgaag gtctcgattt
     1681 gaatgtagaa ctggaaaaag ccctgcgcct ggctgatttt atagagatag gcgagctgat
     1741 ggcccgcgac gcactgcatc gtgaagaatc ctgcggcggc cattttcgcg aagaatacca
     1801 aacccccgaa ggagaggctt tgcgtcagga cgacaagttc aactacgtag cctgttggaa
     1861 atacatgggt gaagataaag aacccgaaat gtataaggaa gagttgaaat atgagttcgt
     1921 acaagtacaa caacgcaatt acaaataata tggaaaagac aataaatata aacttaaggg
     1981 tctggcgtca aagaggtccc aaggccaagg gtgctttcga aagctataag ctggacggag
     2041 tttctaccga cagttctttt ctggaaatgt tggatcaact caatgaacaa ttgatttcag
     2101 aaggcaaaga accgattgtt ttcgatcacg actgccgcga aggtatttgc gggatgtgct
     2161 ccctgtacat caacggccat cctcacggtc cggatgaaga tattaccacc tgccagcttc
     2221 atatgcgcaa attcaaagac ggtgaaacca tcaccattga gccctggcgt tctcacggtt
     2281 tcccggtaat ccgcgacctg atggtggacc gttccgcttt cgataaaatt attcaggccg
     2341 gcggttatgt ttcggtaaat accggaggaa ttcctgatgc caacgctata cccattccta
     2401 aacctgttgc ggacatgtct atggatgccg ctgcatgcat tggatgcggt gcctgtgtcg
     2461 ctgcctgtaa aaatggttcg gccatgttgt ttgttgctgc caaggtcagc cagctggctt
     2521 tgttgcctca gggcaaagta gaggctgcac ggcgggctaa ggcgatggtt cagaagatgg
     2581 acgaattggg atttggcaat tgcaccaata ccggtgcttg tgaggccgaa tgtcccaaaa
     2641 atatttccat aacccatatt gcccgtctga atagagaatt tttgaaggca aaatttaaag
     2701 attaagcagc catggctgtc caggattacg ttcgttccga gtcgttcgac tatccggaag
     2761 ctccggacag ccttgcagcc gatgttaccg aaaattcact gacactacaa aggcctttgt
     2821 acgaaggcat acccagacaa gaaggtcccc tgttcggctt aaagcccttt atcgttttga
     2881 tattgggcct ttttgttttt tcgtatatat tcggacccag aagctcgctc ttccttcatt
     2941 cattaaagga catttttgta taccgcgaac gatccagtat ttttgttgat tccgacgtta
     3001 gattagtacg ttaccggagg ttgctatcgg tttttggctt cctggtttgt cttttgttgt
     3061 tgtctctgat ttttcagccg gaacaaaatg ctagtctaaa cagtttggat gcgaggagct
     3121 taggatatgc cttactcttt ttgggagtgt tcgtattggc aaagttttta ttataccgat
     3181 tttttacttc ttttcagttc gatgtttcca ctgcacggcg atggcaaaac gataatttaa
     3241 ttttgtttat tgtactctct ttctcgtatg ccgttctcgt ttttgtaaat gaaatagtac
     3301 ttataggccc ggagtgtttg ttgaaaatag ccctaatact ctggatttta gacaaaatga
     3361 tacttctggc caagaccgtt cattattttt ccgaccaaag actttcgtgg tttcatttta
     3421 ttatttacct ttgcgcactc gaaattatac ctgcatgtat cttttacaaa ggcatttttt
     3481 gggtacgagg tattgtataa tttaaacgat tatcacgttg aaaatcaaga agatccttgt
     3541 ttctcagcct cagccggcaa cagagaaatc cccctatttt gatattgaga aaaaatacgg
     3601 agtcaagttt gtgtttaggc cttttataaa agtggaaggc ataagcgcca aagagttcag
     3661 gcaacagaaa attgccattc aagattattc tgctgtggtc tttaccgcca ggactgccat
     3721 cgatcatttt tttcgtctgt gtgaagaact cagagtttcg gtatcggaag acctgaaata
     3781 tttttgtata ggggaacaaa ttgctctcta tctgcaaaaa tatattcagt accgtaaacg
     3841 taagattttt taccccgaga gcggcagggt agaagacctg gttgagctta ttcaaaagca
     3901 tagtaacgac aataattttt tggtttcggt atcttacgtt cacaaagacg acttaatttc
     3961 caaactggac gaaaacaaca tcaactacac caaagcggta atgtatcgta cggttagtaa
     4021 tgattttgaa gaaggagaag cttttgatta cgatatgttg attttcttca gcccttcggg
     4081 aattgttgct atgttgaaga attttccaaa ttttgttcag ggggatattc acatagcctg
     4141 cttagggccg actacggcca gagccattcg ggaagcggga ctgaggcttg atctggaagc
     4201 ccccacacca aacgctccct ccattactgc agccttggac caatatttaa aggacaacgg
     4261 caaataacaa aaacggtagc agcagtaaaa ggtaatattc ttttgattta attcaaagac
     4321 actttatttc gctatttttg cagaaataaa gtgtcttttt tttatggata aaagccaact
     4381 atcctttggt aaagcggaac gtttgtgtgg ggaattacgc atcaacaagc tctttgagca
     4441 ggggaaatcg ttcgtggttt atccgctcag ggtttgttgg ttgctgatgc cccctgccga
     4501 aaaagctcct gtacaggtaa tggtgggcgt tcccaaaaaa aagctacgaa aacaggtgca
     4561 gcggaacaga gtaagacgcc ttatcaggga aacctatcgt ctttctaaag gtgatctgct
     4621 caatgaagta cggggaaagg aggctcaact gctgttggca tttatctgga tgtctgaaca
     4681 agtgcctgaa tacaaagacc tggaaacaaa aatgcaggaa gccctccggc agttaagaaa
     4741 aaaactatcg ctatgagaaa gctggcaatt tttttggtcc ttttgccggt acggttttac
     4801 aggttgttta tttctcccct gattccttct tcctgccgct atactccaac ctgctcggaa
     4861 tatgccctgc aggccgtgca gaaatacggc cccgtcaaag gggtccggtt ggccattaga
     4921 aggatattgc gttgtcatcc ctggggaggg catggttacg acccggtgcc ctaatttttg
     4981 aaaccggaat acttatgttt attgatattc atacccatag agaacaacac tacctgaagt
     5041 ttattgagca ggagcaattt gattctcttt cgcctcgctg ggataattcc aggtggtttt
     5101 ttcttaatat agaatccgaa gattttatcc cgggctcgaa tttacctccc tttcttagcc
     5161 taagtacagg gattcaccct tggaaggcag atgttttgcc ggataccgaa aaattagagg
     5221 ctgtttttgc acatcctgac atccgcctga tcggagaaat aggcttagac aaaaggtgca
     5281 cggtgcctta tgaactgcaa tttcaggttt tcgagctgca actggaaatg gccgggacgc
     5341 taaaaaagcc cgtacttatt catcaggtaa gtagtatgga caagattctg gtatgcaaaa
     5401 aagcccatcc cgaaattcca gcttggatta ttcacggttt tagggggaaa aaaacgcagg
     5461 ctttgcaatg gcttcaacac gatttttttc tttctttcgg tcctcaattc aacaatgcgg
     5521 ccttgcaggc ttgtccgccg gagcgcctgt tttttgagac cgatgcgtcg gggatagcta
     5581 tagaagagat atacaaaaaa gccgcacagg tttttgcatg ccctgttgcg gctcttgaat
     5641 tacaggtaga aagaaatgtg aacagcctgt ttaaccaata aacttatagc ctactccttt
     5701 aacggtaaca atgtgattac tgccgatttt ttcacgaatc ttacgaacgt gtacgtcaat
     5761 ggttctgtcg cctactatag aacgattacc ccagacgtgt ctgaagattt cgtcgcgggt
     5821 aaacactttg gtcggccttg aatttaagag ctggagtaat tcaaattctt tgcgaggcag
     5881 gaaaatttct ttgccgtttt tgtacaccag atagcgatcg ctgtcgatga caattccttt
     5941 ttgaggtata acatcgatga ggctgtcttc gtatttggcc aggatattcc tgatgcgctg
     6001 gcagataagg atgctgttgg tgtctctggt accgaattca tctaccccat tcttgaacca
     6061 attaagctgt tcttccagat tactgttgtt gtcgataagg aaatagatgt tgaagcggct
     6121 ttcttccttg agtttcttaa gttttttgca cagatccaat gctttgggat cgtcggtggg
     6181 cgtgtcaaaa atgaccagac aaggagagtc tgtggtgata aaatccaagg ctttctgaac
     6241 gttgtttgtt gtttcaaaat aagctttgcg atctttcaaa aattcctcaa tctgaggagt
     6301 aatggcggac ggttttgtgg caaaaatcaa tgccgagttt ctaagcgttt tcattgtttt
     6361 tgttttaaat gtggatgata tatgcgataa aaataagtga atcgaatagc aatgaatatc
     6421 gttttcttgc taaagcaaca caaatataag atgaagaatc aattaaaaca agttacttta
     6481 tgagaatgat tcaaatttcc cacaaaataa acgtattttt gtgccccagt aattatcatc
     6541 tttactttta gatcaacaat gaacttcatt gaagaattaa gctggagggg tatgatacac
     6601 gatatgatgc cagggacaga ggaacaactc aaaaaagaaa tgacgaccgc ttatgtgggc
     6661 attgacccca ctgccgattc gttacacatt ggtcatttgg tcggtataat gatgcttagg
     6721 catttacagc gctcggggca caaacctatt gctttgatcg gaggtgcaac cggtatgatt
     6781 ggcgatccct cgctcaaatc gggagagcgt aatttgctgg acgaagagac gttgagacac
     6841 aatcaggagg gaataggccg acagttggct aaattactcg attttgaaag taccgctccc
     6901 aatgctgcag aattggtaaa caattacgat tggatgaaag actacagttt tttaaatttt
     6961 attcgggata taggcaaaca catcaccgta aattatatga tggccaaaga ttccgtcaaa
     7021 aaaagactta gcgccgattc caatgtgggc atgtccttta cggaattttc gtaccaattg
     7081 ttgcagggct acgattttct ttacctcaat gaacacaaaa attgcaagtt gcagatgggc
     7141 ggctccgatc aatggggaaa tattaccacg gggacggaat taatacgccg caaaaacggg
     7201 ggagaagcct atgccctgac ctgccctttg attaccaagg ccgacggagg caaattcggc
     7261 aagaccgaat cgggcaatat ctggcttgat ccgcgttata ccagtcccta taaatttgtg
     7321 caattttggt tgaatgtgag cgatgcagat gccgaaaaat atataaagat atttaccttc
     7381 ctcggccgcg aggaaatcga aaacctttgt gtgcaacaca acaaggctcc gcatttgagg
     7441 ttgttgcaaa aaagactggc cgaagaagtt acctgtatgg ttcattccag ggaagaatat
     7501 gaggcagcca ccgaagcggc agcgatactt tttggctcat cctcgaccga acagttgctc
     7561 cgtctggatg aaaaaacctt tttagatctt ttcgaaggaa ttccggtttt tcaggttgac
     7621 cttgatttat ttaaacaagg cgtaaaagcc gttgatctgt tggctgagca tgccgccgtt
     7681 ttcccatcga aaggtgagat gcgcaaaatg gtacaaaatg gcggcttatc tattaataaa
     7741 aacaggtgtc agcaatttga tcagcttttg gatacttcct tcctcttaca tggccgctat
     7801 ctcttgatac agaaaggcaa aaaaaactat ttcttaataa ctgcatcttc cgaaaattaa
     7861 taaaaacact tggttggtaa tataaaaaat acctatcttt gcagtgcttt tttagaaagc
     7921 taaacaggat tgtctcatgg tgtaatggta gcactgcagt ttttggttct gcctgtcgaa
     7981 gt
//
LOCUS       DAOSGL010000066         7972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7802_length_7972_cov_4.228875, whole genome shotgun sequence.
ACCESSION   DAOSGL010000066 DAOSGL010000000
VERSION     DAOSGL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7802_length_7972_cov_4.228875"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2115)
                     /locus_tag="PKY68_03375"
     CDS             complement(<1..2115)
                     /locus_tag="PKY68_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOH24110.1"
                     /translation="MFLVLVLPLALVFSACSGPCFRVSENGVFIRPDQGKTATRSLYL
                     QVVDDDIIRVIASPNRKISLKTGLMCSWQDKAFSQAGRWQVFRTGDSVVLQTAALEVT
                     TLLSSGELSFSDLHGSVFLREQFGGGKSFSPFEYEGCRAYSFRQVFESPPDEAFFGLG
                     QHQSDEFNYKNKHEALYQYNTKVSVPFVVSSNNYGLLWDNNSYSKWGDPREFTNLDQF
                     RLFDKYGKEGGLTAFYSNPGRKKELERVEKAIDYQNLNSIKQFPQAFPLQNALVKWEG
                     SIETDTAGLFRFALYYAGYVKLYLDDTLVVPKHWRTAWNPNTCKFSYPLQAGQKHRIR
                     LEWQPDGGSSYIGLKALSPLPSGEEGKISFWSEMGKQMDYYVVRGRNADEVVSGYRRL
                     TGKAQVMPKWAMGFWQSRERYKTQDELLSVLREFRRRQIPIDNIVQDWSYWEENAWGS
                     HEFDAQRFPNPEQMVRDVHALNARIMISVWPKFYINTEHYKEFEQKGWIYPRAVKDSI
                     RDWIGRGYIGSFYDAFSPGARELFWEQMQEHLYVKGFDAWWMDASEPDILSNADMDYR
                     KQLMHPTALGASALHFNAYGLMNAKGIYEGQRSVNDSSRVFILTRSGYAGSQRFAAAV
                     WSGDIGTRWEDMKAQISAGQNFSISGNPYWTMDNGGFCVEKRYERAREGSDDLEEWRE
                     LNARWHQFGAFVPLFRSHGQYPY"
     gene            complement(2213..3568)
                     /locus_tag="PKY68_03380"
     CDS             complement(2213..3568)
                     /locus_tag="PKY68_03380"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOH24111.1"
                     /translation="MTIKDIIQSRQTQFLDELFSLMRIPSVSGQQAHLQDMQDCAQRW
                     KEILLSSGVDKAEIFQTKGHPIVYGEKIIDPKAHTVLVYGHYDVMPEDPVELWTTPPF
                     EPRIENGLIKGRGADDDKGQSMIQVKAFQLALENNLLACNVKFLFEGEEEIGSIHLEE
                     FCRDHKDLLKADVILVSDTSMLGADTPSITTGLRGISYWEVEVTGAARDLHSGIFGGA
                     VPNPIQELSAIISRLIDKEGKITIPGFYDKVQEASAEERTMLAAVPHDDEAYRKSVGA
                     PGLKGEKGYSTIERTGIRPTLDVCGIWGGYTGEGVKTVLPSKAYAKISTRLVPDQDYQ
                     EIGRLFEDYIRQLAPPEVKVQVRFKHGGNPYVCSLDLPAYKAAEAAYHKVFGKRPLAI
                     RRGGSIPVIATFEKVLGIKSILMGFGLESNAIHSPNENFPLNLWLQSIEVVVEFYRLY
                     K"
     gene            complement(3581..5137)
                     /locus_tag="PKY68_03385"
     CDS             complement(3581..5137)
                     /locus_tag="PKY68_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOH24112.1"
                     /translation="MMKYLQRLGKSLMLPVACLPVAGILLGIGYWLESSLGSNIPATF
                     LLQAGGAILNHIPLLFAVGVAVGMADESNGASALAGLVSWLVITTLLSPATVQMFSGQ
                     TADPAFEKIENVFTGILSGITAAFCYNRFKNTSLPDALAFFGGKRSVPIVAAGLACLI
                     SILLFFSWPLMYNGLILFGKSILDTGPVGAGLYAFFNRLLIPLGLHHALNQVFWFDVA
                     GINDLSNFWTGQGTLGITGQYMTGFFPIMMFGLPAAALAMYHTARPERKKAVGGLMLA
                     SALSAFLTGVTEPLEFSFMFLAPGLYVLHALLTGLTAGIVAYLPVRAGFNFSAGLVDY
                     LLSFKSPMALNPLRIIGIGLLTALVYYLVFRFTIQAFRLRTPGREEENLETPPNEEIR
                     RTLPGKESREALPVGDKDKEGGRKGKKAPAGKGKTTPALGSKNSDNYRETAALLIQGL
                     GGKENIAELDNCISRLRIVIRQDETVDESLLKSAAIAGIVRPGKNTIQLIVGPKVQFL
                     AKEMKQLLTK"
     gene            complement(5134..5391)
                     /locus_tag="PKY68_03390"
     CDS             complement(5134..5391)
                     /locus_tag="PKY68_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOH24113.1"
                     /translation="MTSFTHTIADPLGIHARPAALLVKTASAFRSSIVLRHGDKEISA
                     KKMIALMSLGLKQGDKLEIRIEGEDEEQAGQAMKKILEEQL"
     gene            complement(5401..7035)
                     /gene="ptsP"
                     /locus_tag="PKY68_03395"
     CDS             complement(5401..7035)
                     /gene="ptsP"
                     /locus_tag="PKY68_03395"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOH24114.1"
                     /translation="MRTGKGLGIVNGMASGKLVVVQPRPSSHLEAEKTADPRRELDMY
                     LKAREEALLQLRLLQEQSTATLGKEQAAIFEAQAIMLMDEDFEEAVSSLILKKNLSAT
                     QAVKAGGRKIAALFLGMEDERMRSKSADILDLVQRLHSILSGQTEVPVPPPEAFILLA
                     EDLSPSELIQWRSPLLKGIVLSRGSAHSHLAILAAAMKIPCLISADIEPDQALGNTRI
                     ALDATAGIFYLDPGVEFMSRFNQKLSREDSNGQIPGDVRFYSPRGEEIEVLANIGSPE
                     ELDPDLQKNCQGIGLFRSEFIFMGRRELPSEEEQFELYRRLATGMKGKKVVIRTLDIG
                     ADKDTPCLNLAKEENPALGNRGIRLCLQRPELFRTQLRAVCRAAVYGKLMLLVPMLIS
                     VDELLRVKHLLLDIQQELRQEGLEIPDLPLGAMIETPASVMLCQELAAEADFLSIGTN
                     DLTQYTLAIDRQNPELASLYQQAHPAVLRMLREVIRAAKAAQIPCSICGGLAQDTKHT
                     AMLIDMGFSAFSMAPVHIPGFKKFLYEHYSRSEPSL"
     gene            complement(7039..7515)
                     /locus_tag="PKY68_03400"
     CDS             complement(7039..7515)
                     /locus_tag="PKY68_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HOH24115.1"
                     /translation="MFGFGKTIEILSPIEGSSLPLSECGDEAFAEEMLGKGLAVLPAK
                     GLVVAPFHAKVEVLFDTAHALSLLSDEGVELLIHIGIDTVKLQGKHFKALVKQGDRVR
                     AGEALIEFDPAAIREEGYSTVVPVVVCNSADYAAVESFPGRKLGTLDKPFIRIRKK"
     gene            complement(7565..>7972)
                     /locus_tag="PKY68_03405"
     CDS             complement(7565..>7972)
                     /locus_tag="PKY68_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24116.1"
                     /translation="TALYRHQCRLGLDKNSQLHENNRIVANRIHRFGRHNYSCGGLYT
                     LSAQTGTLIAENYVHDIYRPEYVHDKNQGYYIYLDEASSWMTIRDNWCSLPKFGQNQP
                     GLNHWENNGPQVDESIKLKAGIRPPWQYIKSQP"
BASE COUNT         1815 a         2083 c         2047 g         2027 t
ORIGIN      
        1 gtaggggtat tggccgtggc tgcgaaacaa aggcacaaaa gccccaaact ggtgccaacg
       61 ggcattgagt tcccgccatt cttccaggtc atccgatccc tcgcgggctc tttcgtaacg
      121 tttttccaca cagaaaccgc cgttatccat agtccaatag ggattgcccg aaatggaaaa
      181 attttgtccg gcgctgatct gagccttcat gtcttcccag cgtgtaccga tgtcgccgct
      241 ccagacggct gctgcaaaac gttgtgagcc ggcgtagccc gagcgagtca ggataaaaac
      301 cctgctgctg tcgtttaccg agcgttgacc ttcgtaaatg cctttggcat tcatcaatcc
      361 gtaagcattg aaatgtagag ccgaagcgcc cagagcggtg ggatgcatca gttgtttgcg
      421 gtaatccata tcggcattag acaggatgtc cggctccgaa gcatccatcc accaggcatc
      481 gaagcctttg acgtagagat gttcctgcat ttgttcccag aacagctccc gggcacccgg
      541 actgaaagca tcgtagaatg aaccgatgta gccccggcct atccagtcgc ggatactgtc
      601 cttgacagcc cggggataaa tccagccttt ttgttcaaac tctttgtaat gctcggtgtt
      661 gatgtaaaac ttgggccaga ctgaaatcat tatccgggca ttcagggcgt ggacgtctct
      721 gaccatttgt tccggattgg ggaagcgttg cgcatcaaat tcatgactgc cccaggcatt
      781 ttcttcccag tacgaccaat cctgcacgat gttgtcgatg gggatctgcc ggcgcctgaa
      841 ttcgcggagg acagacaaaa gttcgtcctg ggttttgtag cgctcccggc tctgccaaaa
      901 gcccatcgcc catttgggca tgacctgtgc tttgccggta agcctgcggt aaccgctcac
      961 cacttcgtcg gcgttcctac ccctaaccac gtaataatcc atttgcttgc ccatttccga
     1021 ccagaaagaa atttttcctt cttccccgga cggtagggga ctcagggctt tgagtcctat
     1081 gtaggaactg cctccgtcgg gctgccattc cagcctgatc cggtgttttt ggccggcctg
     1141 cagaggatac gaaaacttgc aggtgttggg attccaggcg gtacgccagt gcttcggtac
     1201 caccagcgta tcgtcgaggt acagtttgac gtagcccgcg taatagagcg caaaacgaaa
     1261 caggccagca gtatctgtct cgatggatcc ttcccatttt accagggcat tttgcagcgg
     1321 gaaagcctga ggaaactgtt ttatagagtt caaattttgg taatcgatgg ctttttcgac
     1381 acgttccaat tctttttttc ggccgggatt cgaataaaag gcagtcagcc cgccttcttt
     1441 accgtacttg tcgaacagcc tgaattgatc taagttggtg aattcccggg gatcgcccca
     1501 tttgctgtaa gaattgttgt cccagagcag gccgtagttg ttgctcgaaa ccacaaaagg
     1561 caccgagacc ttggtgttgt actgatacag ggcttcgtgt ttgtttttgt aattgaattc
     1621 gtccgactga tgctgaccca ggccgaaaaa agcttcatcc ggaggcgatt caaatacctg
     1681 cctgaaagaa taagcccgac atccttcgta ttcgaacgga ctaaagcttt tacccccgcc
     1741 gaactgttcc cgcaaaaaca ccgaaccgtg taagtcgctg aaactaagtt ctccgctact
     1801 caatagagtg gtgacttcca gggcagccgt ttgcagtacc acactgtctc cggtacgaaa
     1861 cacttgccat ctacccgctt ggctgaatgc tttgtcttgc cagctgcaca tcagtccggt
     1921 tttcaggctg atttttcggt tgggactggc tataaccctg attatatcgt catcgaccac
     1981 ttgtaaatag agactgcggg tggcggtttt cccctggtcg gggcggataa atactccgtt
     2041 ttcgctgacc cggaaacagg gtccggaaca ggcagaaaac accagagcga ggggcagaac
     2101 cagaaccaga aacaaaaacc taaacttagg cctagaccta gacctagacc caacctcagc
     2161 cctaagcccg gactcggacc gaaaccccaa ggagcgacta ttcatgatgc aattacttat
     2221 aaagacggta aaattccaca acaacttcga tgctttgcag ccaaagattc aagggaaaat
     2281 tttcgttggg ggagtggata gcgttggatt ccaggccgaa gcccatcagg atgcttttga
     2341 ttccgagcac tttttcgaaa gtggcaatga caggtatgct gcctccgcgc cttatggcca
     2401 gcggacgttt gccaaacacc ttatgatagg cagcttcagc cgctttatag gccggcaggt
     2461 ccaaagaaca aacgtaggga ttgcccccgt gcttaaagcg aacctgcacc ttgacttcgg
     2521 gtggagccag ttgcctgatg tagtcttcga agagccggcc tatttcctga taatcctgat
     2581 ccggcaccag gcgggtagat attttggcgt aagccttgga aggaagcacc gttttcactc
     2641 cttcgccggt gtagcctccc cagatgccgc aaacatccaa cgtgggtctg atgccggtgc
     2701 gttcaatggt agaatagcct ttttctcctt tcagaccggg ggctcccacc gatttacgat
     2761 aggcttcgtc gtcgtgaggc acggcggcca acatggttct ttcttcggcc gatgcctcct
     2821 gcaccttgtc gtaaaaaccc ggtatggtga ttttaccctc cttatcgatc aggcgggaga
     2881 ttattgcgga cagttcctgt atggggttgg gaacggctcc gccgaagatg ccggaatgca
     2941 aatcccgggc agcgccggtc acttccacct cccaatagga aatgccccgc agccctgtgg
     3001 taatggaggg agtgtcggct cccagcatgc tcgtgtcgga aacgaggatc acatcggcct
     3061 tgagtaagtc tttatgatct cggcagaatt cctccagatg gatggaaccg atttcttctt
     3121 cgccttcgaa cagaaatttt acgttacaag ccagaagatt gttttcgaga gccagttgaa
     3181 aagccttgac ctgtatcata gattgccctt tgtcgtcatc ggcgccccgg cctttgatca
     3241 gcccgttttc gatgcggggc tcaaagggtg gcgtagtcca cagttccacc ggatcttcgg
     3301 gcatcacgtc gtaatggccg tacacgagca ccgtgtgagc cttgggatcg atgatttttt
     3361 ctccgtacac aatgggatgc cccttggttt gaaaaatctc ggctttgtcc actcccgaag
     3421 agagtaaaat ctctttccaa cgttgtgcac aatcttgcat atcctgtaaa tgagcttgct
     3481 gtccgctcac cgaaggaatg cgcatcaggc tgaacaattc gtcgaggaat tgcgtttgcc
     3541 gggactgaat aatgtcttta atagtcatat agataggctt ttatttagta agcagttgtt
     3601 tcatttcttt ggccagaaac tgcactttgg gaccgacaat caactgaatg gtgtttttgc
     3661 ccggccggac tatgcccgca atagcagcgg attttagtag gctttcgtcc accgtttcgt
     3721 cttgtctaat cacaatgcgg agccgcgaga tgcagttgtc cagttcggca atgttttctt
     3781 tgccgccgag gccctgtatc aggagagccg ctgtttcccg gtaattatcg ctgttcttgc
     3841 tccctagggc cggagttgtt ttgccttttc cggccggagc ttttttgccc tttcggccac
     3901 cctccttgtc tttatctccg accggcaggg cctcccggct ttctttgccc ggtagagtcc
     3961 ttcgaatttc ttcgttcgga ggagtttcca ggttttcttc ttccctgccc ggtgtgcgta
     4021 gacggaaggc ttgtatcgta aaacggaaga ccaggtaata gaccagggct gtcaggagtc
     4081 ctattccgat tatccggagg ggattcagag ccataggcga tttaaaactc agcagataat
     4141 ccaccaggcc ggcactgaaa ttaaaacccg ccctgaccgg cagataagct acgatgcctg
     4201 cggtcagtcc cgtcagcaag gcgtgcagga cgtacaggcc gggagccaga aacataaaag
     4261 aaaactccag aggttcggtt accccggtca ggaaagcact cagagcggag gccaacatca
     4321 gtcctcccac cgctttttta cgttcgggtc tggcggtgtg gtacatagcc agggcagcgg
     4381 caggcaggcc aaacatcatt atcgggaaga agccggtcat gtattgacct gtaatcccga
     4441 gtgtgccttg cccggtccaa aagttgctca ggtcgttgat gcctgccaca tcgaaccaga
     4501 agacctgatt cagcgcatga tgtaagccca gcggaatgag caggcggttg aaaaaagcgt
     4561 acagtccggc gcccacaggc ccggtatcca aaatactttt accaaagaga atcaacccgt
     4621 tgtacatcag gggccagcta aaaaacaaaa gaatacttat caaacaggcc aggccggccg
     4681 ccacgatcgg gacagagcgt ttgcctccaa aaaaagccag ggcatcgggc agggaagtgt
     4741 ttttgaaacg gttgtagcaa aaggcagctg ttatgcccga aagaataccc gtaaaaacgt
     4801 tttcgatttt ttcgaaggcc ggatcggccg tttgtccgct gaacatttgg acagtggccg
     4861 gagagagtaa agtagttata accagccaac tgaccagtcc ggccagggca gaggctccgt
     4921 tgctttcgtc ggccattccc acggctacac ccacggcaaa caataggggg atgtggttga
     4981 gtatggctcc cccggcctgt aataagaaag tagccggaat attgctgccc aacgaagatt
     5041 ccaaccaata gccgataccc agcaaaatac cggccaccgg cagacaagcc accggcaaca
     5101 tcagcgattt gcccagtctt tgcaggtatt tcatcatagc tgttcttcta agatcttttt
     5161 catagcctgg ccggcttgct cttcgtcttc tccttcaata cgtatttcga gcttatcgcc
     5221 ttgtttaagg cccaggctca tcaaagcgat catttttttt gcactgattt ccttatcccc
     5281 gtggcgcagg acgatcgaag aacgaaaggc agaagctgtt ttaaccaaca gggcggccgg
     5341 cctggcatgg atgcccagcg gatcggcgat cgtatgtgta aaacttgtca tagcgtgggt
     5401 ttaaagcgaa ggttcggagc gggaatagtg ttcgtacagg aattttttga agccgggaat
     5461 atgcaccgga gccatggaga atgcgctgaa tcccatgtct atcagcatgg ctgtgtgctt
     5521 tgtgtcctga gccagtcctc cgcagatgga gcagggaatt tgcgcagcct tagccgcccg
     5581 gatcacctct cgaagcatac ggagcacggc cggatgtgcc tgttgataca gagaggcaag
     5641 ctccgggttt tgccggtcga tggccagggt gtattgtgtc aggtcgttgg tgccgatgct
     5701 gagaaagtcg gcctctgctg ccagttcttg gcacaacatc acagaggcgg gtgtttctat
     5761 catggcaccc aggggcaggt cggggatttc gagcccttct tgcctaagct cttgttgaat
     5821 gtcgagcaac agatgtttta cccggagtaa ttcgtcgacg gaaatcaaca tggggaccaa
     5881 cagcattagt ttaccgtaaa ccgcagcccg gcaaaccgct ctgagctgag tcctgaaaag
     5941 ctccggcctt tgcaggcaca gccgtatgcc cctgtttccc agagcaggat tttcttcttt
     6001 ggctaagttt aaacagggag tgtctttgtc ggcacctata tcgagggttc tgatcaccac
     6061 ttttttgccc ttcatgcccg tagccaaacg gcgatacagc tcaaactgtt cttcttcgga
     6121 ggggagctcc cggcgtccca taaaaatgaa ttcgctgcga aacaaaccta tgccctgaca
     6181 atttttttgc aagtcagggt cgagttcctc cgggctgccg atattggcca gtacttcgat
     6241 ttcttctccc cgggggctgt aaaaccgcac atcgccggga atttgtccgt ttgaatcttc
     6301 tcgactgagt ttctgattga aacggctcat aaattcaact cccggatcga gataaaaaat
     6361 cccggcggtg gcatcgaggg cgatccgggt atttccgaga gcctgatcgg gttcgatatc
     6421 ggccgagata aggcagggta ttttcatagc ggctgccagt atggccaggt gagaatgagc
     6481 cgatccgcgg ctcagtacaa ttccctttaa cagcggtgaa cgccattgta tcaattcact
     6541 tgggctcagg tcttcggcca ggagaataaa agcttccggg ggcggaacgg gaacctctgt
     6601 ttgtccgctc aatatcgaat gtaagcgttg taccaggtcg agaatatcgg ccgatttact
     6661 gcgcatccgt tcgtcttcca tacccagaaa cagggcggct atttttctac cgcctgcttt
     6721 gaccgcttgc gttgcactta gattcttttt cagaatcaga gagctgacag cctcttcgaa
     6781 gtcttcgtcc ataagcatta tagcctgagc ttcgaaaatg gcggcttgtt ctttgcccaa
     6841 tgtggctgtg ctctgttctt gtaaaagccg gagttgtagc agggcttctt ccctggcttt
     6901 taaatacatg tccagttcgc gccggggatc ggctgttttc tctgcttcaa gatgtgacga
     6961 tggccggggc tgaacgacaa caagtttgcc gctcgccatt ccattcacta ttccaagtcc
     7021 tttacctgta cgcatgtgtt atttttttcg gattcgaata aaaggtttat cgagtgttcc
     7081 tagcttccgg cccggaaaac tttcaacggc ggcgtaatcg gcgctgttgc aaaccaccac
     7141 agggacaacc gtgctatagc cctcttcccg gatggcggca ggatcgaatt cgatcaatgc
     7201 ttctccggct ctcacccggt caccctgttt taccagggct ttgaagtgct taccttgcaa
     7261 ttttacggtg tcgataccga tatgtatcag caactcaacg ccttcatcgc tgagcaggct
     7321 cagggcatgg gctgtatcaa acaacacttc tactttggca tgaaaggggg ctacaaccag
     7381 gcctttggca ggtagtaccg ccagaccttt gcccagcatt tcctccgcaa aagcctcatc
     7441 accgcactcg ctcaaaggga ggctgctgcc ttcgatcgga gataaaattt ctatggtctt
     7501 gccaaatcca aacatttcaa gagtttaggc tatactttcg gaattgttta ttcaacagtt
     7561 ggtgtcaggg ctgtgatttt atatattgcc acggagggcg aatgcccgct tttaatttaa
     7621 tactttcatc aacctgaggc ccgttgtttt cccagtgatt caggccgggc tgattttgac
     7681 cgaatttggg caggctgcac cagttgtcgc gtatggtcat ccacgaggaa gcttcgtcga
     7741 gataaatgta atagccctga tttttgtcgt gcacgtattc gggacgatat atatcgtgta
     7801 cgtaattttc ggctatcaac gttcctgttt gggccgacag ggtgtacaag cccccgcagc
     7861 tgtaattatg tctgccgaaa cgatgtatcc ggttggctac gatacggtta ttttcatgca
     7921 gttggctgtt cttgtccagc cccagcctac actgatgccg gtatagggca gt
//
LOCUS       DAOSGL010000067         7919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7885_length_7919_cov_4.050865, whole genome shotgun sequence.
ACCESSION   DAOSGL010000067 DAOSGL010000000
VERSION     DAOSGL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7885_length_7919_cov_4.050865"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKY68_03410"
     CDS             <1..659
                     /locus_tag="PKY68_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24117.1"
                     /translation="LNSGAEGVADDIYTLSLYESAHKYAGFTMIQAKEYETAFEYFSK
                     YASFGRSDTPTDGNFLFNYANLARQCGKDSLALDLYDQCIELKYRDDASLYSKALILK
                     DREDVEQMKEVLLKGLENYPESRYASSISGLLSAQLMREASKPFNEANSIAGTASSTN
                     IDAYISTMTQATEKYKEAITLFEEVLKYDPENKNATNYINLSKENVKRFEDYKKSIGR
                     "
     gene            complement(749..1183)
                     /locus_tag="PKY68_03415"
     CDS             complement(749..1183)
                     /locus_tag="PKY68_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOH24118.1"
                     /translation="MTTTIRKFQNHWFPSLFNDMFDKEFTVSAPICTPAVNVIEDEKS
                     YRIEMTAPGMTKKDFSIRLDENDVLVVEMKKTTDKKEEDKKLNYLRREFAYTEFKRSY
                     YLPEDVDKKNIKASMENGVLNIELHKKTEAQKASEVKRIEIS"
     gene            1445..1858
                     /gene="ruvX"
                     /locus_tag="PKY68_03420"
     CDS             1445..1858
                     /gene="ruvX"
                     /locus_tag="PKY68_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOH24119.1"
                     /translation="MGRILAIDYGRKRCGIAVSDPLKLIANGLKTVATQDLDAFFEQY
                     LKEETVDLVVVGLPKQLNNEPSEAMSYIEPYVRKFKKKYPEIPLVYSDERFTSVLAHK
                     AMLEGGLKKKDRRNKALADEVSATILLQDYMEFNK"
     gene            1862..2416
                     /gene="def"
                     /locus_tag="PKY68_03425"
     CDS             1862..2416
                     /gene="def"
                     /locus_tag="PKY68_03425"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOH24120.1"
                     /translation="MQLPIYLYGQSVLRKEAEEIGPDYPGLPELISNMQDTLEQAEGV
                     GLAAPQVGLPIRLFIVDLRPLADQEPSFSGYTRSFINPKILEYSEDTEILEEGCLSVP
                     GIQENVARSRKIVLQYQDTDFKQHKEVFEDYVARVIQHEYDHLNGKVFTDRINPIRKQ
                     LVKGKLNNIAKGRVECTYKTRALR"
     gene            2450..4564
                     /locus_tag="PKY68_03430"
     CDS             2450..4564
                     /locus_tag="PKY68_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH24121.1"
                     /translation="MTNRDGDKIVNRGLLTALLLLITLLNPWSVTKSQINTDRMMIIG
                     RNALYFEDYILSIQYFNRVIQSKPYLAEPYLYRAIAKLSLEDFLSAEEDCTAALERNP
                     FIVRAYYVRSYARMHLKKYEAAIEDCEQGLEFNTEHKGLMLNKGISRMYAGQHREARN
                     DLDELLRKDPNFLYAYMSRGQLSVETGDTLGAVDDFSRALAVDRYFAPAYASRAYVNL
                     LRKNFTDAISDFNEAIHLEPDNESYFINRSLARYYLNDYRGSLNDLDRVVQINPNSLT
                     AYFNRGLIRAEVGDDNRAIEDFDKVIFMEPGNDLAIYNRAVLRSNTGDLEGAEADLSL
                     IIDRYPNFYPAFYQRSELRKKMRDTKGADKDYFAAWDLENKIREQRAAGKEVSAAGDR
                     GEDEKTREETDKDLFKYNRVIVPPNELAQSSRYANPIRGRVQDQQVRLALEPMYRLSF
                     YEELHPVGMKRPMNYRHYLESLDQSDVRFEGLLISNRCKSLSEEQVEKHFESIDLYSK
                     LIGENAGRAVLFFARALDFALVKDNDNALLDLNRALFLDKDFVLAYFERANLRFDLLM
                     FEQSQNIQEEIQLSAYSTKKTDEDKQSGSKLTPVGEEAFGAEYILVMQDYEKVLEIAP
                     EFVYAWYNRGYIRFLQKDYRSALEDFNRAIELSPDFAEAWYNRGIIRIYLGERENGLR
                     DLSKAGELGMYQAYNLIKRFTE"
     gene            complement(4571..5635)
                     /locus_tag="PKY68_03435"
     CDS             complement(4571..5635)
                     /locus_tag="PKY68_03435"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HOH24122.1"
                     /translation="MEKHNAVLLLCTGGTISMSEDPVTGALRPVNFEKIFDYLPELNK
                     IGIRTDSIAFDPLIDSSDVDPSIWSKLAHIIYDHYHQYDGFVVLHGTDTMAYSASALS
                     FMLNNLSKAVIFTGSQLPLGVLRSDARENLLTAIEIAAARENGRPIVPEVCIFFEDTL
                     LRGNRTTKQNAEHFNAFCSHNYPPLAKAGVHIRYAKKFIHYPDPQSSLSLRTRIDQQV
                     AVLKIFPGISEKTVRAILEMPGLKAVVLETFGSGNAPRQEWFRKALSEANDKGIILVN
                     KTQCAVGSVEMGRYETSLDLLNAGVLSGMDITTEAIVTKLMYLMGEHNSTEEVRKLLQ
                     TNLCGEISLEDEQAYLEPLR"
     gene            5765..7303
                     /locus_tag="PKY68_03440"
     CDS             5765..7303
                     /locus_tag="PKY68_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH24123.1"
                     /translation="MSRFASAALRTALSLLLFLLLPVLAIKAETKVLINEFLSINNGI
                     IQDEDGENSDWIELYNAGEDLVNLENWSLTDDSTNLQKWLFPSVNLEAGAYLLVFASD
                     KDRRTENEELHTNFKLASSGEFLALVEPGALVPAFAYSPAYPPQRSNVSYGIYQGQLC
                     YLENPSPGSENLLGDQVLPPVFSKPADFYHQAFELELSVALEGQEIYYTTDGSLPNKE
                     QAQLYTTPLSITTTTVVRAVCITEDGRLSPSVSRSFIFLEDVLRQGQQPAGYPQVWND
                     STYPADYEMDAGICQHPDYENQILDALLELPSISIMLHPDSLFNLDRGIYVNSEKKGD
                     EWERPASMEYLNPKTGESFQINAGLRINGGNSRKPGNSPKRSFRFYFRKSYGFPKLNY
                     PIFDEETATDRFDHLILRAGYNYTWIKNGTDGQHSYAVQRPMAQYIIDPFAKETQLAM
                     GHLAVHNRFVHLYINGLYWGVYDLNERVKSEFAEAYLGGDEEDYDVVWPATKTTKELW
                     TVLK"
     gene            7255..>7919
                     /locus_tag="PKY68_03445"
     CDS             7255..>7919
                     /locus_tag="PKY68_03445"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOH24124.1"
                     /translation="MASDKDDKGIVDGIEIDDDGDGKPDRFLGRDIFNEMYGIATQVG
                     SSPDDSNYARIISEKLLDLEGFIDYMLINFYIGNSDWDRGNWYALRHRYRPDKGFRFL
                     CWDAETALIDVYDNKVSMRDGEPTKILAGLKNNPEFRLLFADRVYKHLFNGGLLTPEA
                     AAARYEKLASEIEKPLIAESARWGDYRKMNNETTVVYTPNDHWKPRKESLLKDYFPQR
                     TAI"
BASE COUNT         2172 a         1702 c         1889 g         2156 t
ORIGIN      
        1 acctgaattc cggtgcagaa ggtgttgccg acgacatcta caccttgagt ctttatgaat
       61 ctgcgcataa gtatgccggt ttcaccatga tacaggccaa agaatacgaa acagcctttg
      121 agtatttttc caagtacgcc tctttcggcc gttccgatac tcccaccgac ggcaacttct
      181 tgttcaacta cgccaatctg gcccgtcagt gtggaaaaga ctctcttgcg ctggatttgt
      241 acgatcaatg tattgagctt aaataccgcg acgatgcttc tctttactca aaagcactaa
      301 ttcttaagga tagagaagac gtggaacaaa tgaaagaagt attgcttaag ggtctggaaa
      361 attatcctga aagcagatac gccagtagta tatccggctt gttgagtgca caactcatgc
      421 gtgaagcctc caaacctttc aatgaagcga actctatagc ggggacagct tccagtacca
      481 acatagatgc ttacatatcg acaatgaccc aagccactga aaagtacaaa gaagcaatta
      541 ctttgtttga agaggtattg aaatacgatc ctgaaaacaa gaatgctacc aactacatca
      601 atcttagcaa agaaaatgtc aaacgtttcg aagattataa gaaaagcata ggacggtaaa
      661 gcattgcatg aatatagaaa tagaaacggg gactctgttt tatacaacag gatccccgtt
      721 ttctttggac taatacccga gagatatatt aactgatctc gatgcgtttt acttcgctgg
      781 ctttttgagc ctcggttttt ttgtggagtt caatattaag tactccattt tccatactgg
      841 ctttgatgtt tttcttgtct acatcttcgg gaaggtagta gctccgctta aattccgtat
      901 aggcaaattc acggcgcagg taattgagtt ttttgtcctc ttcctttttg tcggtggttt
      961 ttttcatttc taccaccaaa acatcgtttt cgtccagtct gatgctgaaa tcttttttgg
     1021 tcatgcccgg agcagtcatt tctatgcgat aggatttctc gtcttcaata acgttgacag
     1081 cgggagtgca aattggagca ctgactgtga attctttatc aaacatgtcg ttgaacaggc
     1141 tcgggaacca gtggttttga aattttctga tagttgttgt catagctgta agttttaaaa
     1201 gttatattat ttttttttat tcatcactac tctataactc acaaatcttg tgcctgaagc
     1261 atattggcga caaagtggca gttgttggtg acaattttgc agatataatt gtttattaca
     1321 ctattaatca gtagattaat ttaatcagat tgttttttta atggctttaa ccgatttttt
     1381 tgagcccgga atcctgtgca agaacaataa ttagtgtatt tttgtgtttg gtttttaaaa
     1441 agatatggga agaattctgg ccatagatta tgggcgaaaa cgttgcggaa tagctgtaag
     1501 cgatccctta aaacttattg ccaacggatt gaaaacggtt gcgactcagg atttggatgc
     1561 ctttttcgaa cagtatctca aagaagaaac agtcgacctg gtggtggtgg gtttgccaaa
     1621 acagctcaac aacgaaccct ccgaagccat gagctatata gagccttatg tcagaaaatt
     1681 caaaaaaaaa tatccggaga tcccattggt gtattcagat gaacgtttca cttccgtgct
     1741 ggctcacaaa gcgatgctgg agggaggatt gaagaagaaa gaccgtcgaa acaaagcctt
     1801 ggccgacgaa gtgagcgcca ccatattgct tcaggattac atggaattta acaaataaat
     1861 tatgcaacta cccatttatt tatacggcca aagcgttttg cgtaaagagg ctgaagaaat
     1921 cgggccggat tatcccgggc tccctgagct catcagcaat atgcaagaca ccctggaaca
     1981 ggccgaaggc gtcggcctgg ctgctcctca ggttggcctg cccatcagac tttttattgt
     2041 tgacttaaga ccgctggccg accaggaacc ctctttttcc ggctataccc gaagctttat
     2101 caatcctaag attttggaat attccgagga tacggaaatt ttggaagagg gttgcctgag
     2161 tgtgccgggt attcaggaaa atgtagcgag gagtagaaaa attgtgttac aatatcagga
     2221 taccgatttt aagcaacaca aggaagtctt cgaagattat gtggccagag taattcagca
     2281 cgaatacgat catctgaacg gcaaagtttt caccgaccgt attaatccta ttcgtaagca
     2341 actggtcaaa ggaaagctca acaatattgc aaaaggcagg gtggaatgta cttataaaac
     2401 cagagctttg cgttgagcat tttaaaaaaa tatcagaaga cagcaataca tgacaaaccg
     2461 ggatggggat aaaatagtga accggggctt gctaacagct ctgctgttgc tgataacctt
     2521 attgaatccg tggtctgtga caaaatcgca gatcaacacc gaccggatga tgatcatcgg
     2581 ccggaatgcc ctgtatttcg aagattacat cctttccata cagtatttca atcgggtaat
     2641 acaatcaaag ccctatctgg cagagcctta cttgtaccgg gcgatcgcta agctgagtct
     2701 cgaagatttt ttgagtgccg aagaagattg tacggccgcg ctcgagcgta atccctttat
     2761 cgtcagggct tattatgtca gaagctatgc ccgtatgcat ctcaaaaaat acgaggcagc
     2821 catagaagat tgcgagcagg gcctggaatt caacaccgaa cacaaaggat tgatgctgaa
     2881 taaaggtatt tccaggatgt acgccggtca gcaccgggaa gcccgcaacg acctggatga
     2941 attattgcgc aaagacccta attttctgta tgcttacatg agccggggac aattgtcggt
     3001 ggaaacaggc gacacgctgg gagccgtcga tgattttagc cgggctctgg ccgtggatcg
     3061 ttattttgct cctgcctacg cttcgagggc ttatgtcaat ttgttgcgca aaaattttac
     3121 cgatgccatt agtgatttca acgaagccat ccacctggaa cccgacaacg aatcctattt
     3181 tatcaacaga tctctggctc gttattatct gaatgattac agaggctccc tgaacgattt
     3241 ggaccgggtg gtacaaatca atcccaattc cctgacggct tacttcaacc gcgggttaat
     3301 cagagcggaa gtaggcgacg acaacagagc tatcgaggac ttcgataaag ttatttttat
     3361 ggaacccggt aacgatctgg ccatatacaa tcgtgcggta ttgcggtcga acaccggaga
     3421 tctggagggt gctgaagcgg atttgagctt gataattgac cgttacccca atttttatcc
     3481 cgccttttac caacgctcgg aattgcgtaa aaaaatgcgg gacaccaaag gagcagacaa
     3541 agattatttt gccgcctggg atcttgaaaa caagatacgt gaacaacgtg ctgccggcaa
     3601 agaggtcagc gccgcgggcg acagggggga ggatgaaaaa acccgcgaag aaacagacaa
     3661 ggatttattt aaatacaacc gggtgatagt gcctcccaac gaactggcgc agtcgagccg
     3721 ctatgccaat ccgatcaggg gcagagtaca ggatcagcaa gtccggcttg ccctggagcc
     3781 catgtatcgt ttgagttttt atgaggaatt acatcccgta gggatgaaac gccccatgaa
     3841 ttaccggcac tatctggaat cgctcgatca gagcgatgtt cgcttcgaag gtctacttat
     3901 ttccaaccgt tgcaaatcac ttagtgagga acaagtcgaa aagcattttg aatctattga
     3961 cctctattcc aaattgatag gagaaaacgc cggaagggcg gtactgttct ttgccagggc
     4021 cttggatttt gctttggtca aggataacga taatgcactg ctcgacctga accgtgcttt
     4081 gtttttggat aaggatttcg tgttggccta ttttgagaga gccaacttgc gtttcgactt
     4141 gcttatgttt gaacagtcgc agaatattca ggaagaaatt caactttcgg cgtattcaac
     4201 taaaaaaacg gacgaagata agcagtcggg ctccaaactt actcccgtag gcgaagaggc
     4261 atttggagcg gaatatatac tggtaatgca ggattacgag aaagtgctcg aaatagctcc
     4321 cgaatttgtt tatgcctggt acaacagagg ctacatccgc tttttgcaga aggactatcg
     4381 cagtgctctc gaagacttca accgcgccat agaactcagt cccgatttcg ccgaagcctg
     4441 gtacaacaga ggaatcatac gtatttatct gggcgaacgc gaaaacggct tgagagattt
     4501 gagtaaagca ggagaattag gtatgtatca ggcgtataac cttattaagc gatttacgga
     4561 atagggcgtt ttatcgtaag ggttccaagt aggcctgttc gtcttcgagg ctgatctccc
     4621 cgcaaaggtt ggtttgcaga agttttctta cctcttccgt agaattgtgt tctcccatca
     4681 aatacatcag cttggtcact atggcttcgg tggtaatgtc cataccgctc agtacaccgg
     4741 cattcagcaa atcgaggctg gtttcgtagc gtcccatttc tacggagcct acggcacatt
     4801 gggttttgtt cacaaggata atgcccttgt cgttggcttc cgaaagggct tttcggaacc
     4861 attcctgccg gggagcattt cctgaaccga aggtttcgag tactaccgct ttcaggccgg
     4921 gcatttcgag gatggctctc accgtttttt ctgaaattcc cggaaagatt ttcagtacgg
     4981 ccacttgttg gtcgatgcgg gtacgtaggc tgagtgagct ctgaggatcg ggataatgta
     5041 tgaatttctt ggcgtatcta atgtgtactc cggccttggc caaaggagga taattgtgcg
     5101 agcaaaatgc gttaaaatgc tctgcgtttt gcttggtagt tctgttgcct ctcaacaaag
     5161 tgtcttcgaa aaaaatgcaa acttcgggta ctataggccg gccgttttct ctggctgcgg
     5221 caatttcaat ggcagtcagc aggttttcgc gtgcatcgga acgcaatacc cccaagggca
     5281 attgtgagcc tgtaaaaatg accgctttgg acaggttgtt cagcataaag ctcagagccg
     5341 aagctgagta agccatggtg tccgttccgt gcaataccac gaaaccgtcg tattgatggt
     5401 agtgatcata gatgatatgt gccagtttgc tccagatcga aggatcaacg tcggaggagt
     5461 ctataagcgg atcaaaagca atggaatccg tcctgattcc gatcttgttt aattcgggca
     5521 ggtaatcgaa aattttttca aaattgacag gtcgcagcgc tccggtaaca ggatcttcgg
     5581 acatgctgat ggtgccgccg gtacagagta gcaatactgc attatgtttt tccacggctg
     5641 tcgttttatg tacgcaaaaa tagacaatta ggaacaaata caaaattttg cctatttttg
     5701 ccggcttaat tcttgatttt tgtaccgttt gaataactct gactcagatt tttttctctt
     5761 tcgtatgtcg cgatttgcat ctgctgcttt gaggacggct ttgtctttat tattgtttct
     5821 tttgttgccg gttcttgcta ttaaagctga gactaaggtg cttatcaatg aatttttgag
     5881 tattaacaac ggcattattc aagatgagga cggtgaaaat tccgactgga tagaattgta
     5941 caatgcggga gaagatcttg tcaatctgga gaactggtct ttgaccgatg attcgacgaa
     6001 tttacagaaa tggctgttcc cctccgtcaa tctggaagcc ggagcctatt tgctggtttt
     6061 tgcttcggat aaagacagga ggacggaaaa cgaagaattg cataccaact ttaaattagc
     6121 ctcttcgggt gagtttctgg ccttggttga gccgggagct ttggttcctg cctttgctta
     6181 ttcaccggct tatccccctc agcgaagcaa tgtgtcttat ggtatctatc agggacaatt
     6241 atgttatctc gaaaatccca gtccgggctc cgaaaattta ctcggagatc aggtgttgcc
     6301 gccggttttc agcaagcctg ccgattttta ccatcaggct tttgaattgg agctgagtgt
     6361 ggcgctcgag ggccaggaaa tttattatac taccgacggc agtttgccca acaaagaaca
     6421 agctcaactg tataccaccc ctctgtcaat cactactacg acagttgtcc gggctgtgtg
     6481 cataaccgaa gatgggcgcc taagtccttc cgtgagcaga tcttttattt ttttggagga
     6541 tgtactcagg caaggtcaac agcctgccgg ttatcctcag gtatggaacg acagtactta
     6601 tccggccgat tacgagatgg atgccggtat ttgccaacat cccgattacg agaatcaaat
     6661 actcgatgcc ctgcttgagt tgcccagcat cagtataatg ttgcatcccg attccctgtt
     6721 taatttggat aggggtattt acgtgaattc cgagaaaaaa ggagacgaat gggagcgtcc
     6781 tgcctccatg gaatacctta atcccaaaac gggggaatcg tttcaaataa atgccggttt
     6841 aaggataaac ggaggtaaca gccgcaagcc gggaaacagt cccaaacgtt ctttccgttt
     6901 ctatttcaga aaatcttacg gttttcccaa attgaattat cccatcttcg atgaagaaac
     6961 ggccaccgat cgtttcgatc atcttatatt acgtgccggt tacaattata cctggatcaa
     7021 gaacggtacc gacggtcaac acagttatgc cgtacaacgt cccatggccc agtatatcat
     7081 tgatcctttt gccaaggaga cgcagttggc tatggggcat ctggccgtgc acaatcgttt
     7141 tgtgcatctc tacatcaacg gattgtactg gggagtctac gatttaaacg aacgggtcaa
     7201 aagcgaattt gccgaagctt atctgggagg tgatgaagaa gactacgatg tagtgtggcc
     7261 agcgacaaag acgacaaagg aattgtggac ggtattgaaa tagacgacga cggcgatgga
     7321 aaacccgatc gctttttggg aagagatatc ttcaacgaaa tgtacggtat tgccactcag
     7381 gtggggagca gccccgacga ctctaattac gccaggatta tctcggaaaa actcctggac
     7441 ctagaaggct ttatcgacta catgctgatc aatttttata tcggaaacag cgattgggac
     7501 aggggaaatt ggtatgcact gagacaccgt taccggccgg ataaaggttt tcgctttctg
     7561 tgctgggatg ccgaaacagc cctgatagat gtgtacgata acaaggtctc gatgagagac
     7621 ggtgaaccca ccaaaatcct ggcaggcctg aaaaataatc cggaattccg gttgcttttt
     7681 gccgaccggg tgtacaaaca tctgtttaac ggggggctgc tgactcccga ggcggcagcg
     7741 gctcgttacg aaaaactggc ctcagaaatc gaaaagcctc tgatagccga atcggcccgc
     7801 tggggcgatt accgtaagat gaacaatgaa acaacagtag tgtatactcc caacgaccat
     7861 tggaagcccc gaaaggaaag tttattgaaa gattattttc cccaacggac agccatttt
//
LOCUS       DAOSGL010000068         7852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7990_length_7852_cov_3.858407, whole genome shotgun sequence.
ACCESSION   DAOSGL010000068 DAOSGL010000000
VERSION     DAOSGL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7990_length_7852_cov_3.858407"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PKY68_03450"
     CDS             <1..871
                     /locus_tag="PKY68_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH24125.1"
                     /translation="GIRAAVGVTFLESNGAKYIERGLRLLENLPETSSRVQITVAPHA
                     VYTVSEDLFKRLAAMARERSLIIHTHLAETRQEVQDCIAAHGMSPVRWLDHLGLLTPN
                     TVAAHVVHADDYELDLLRDRGVWLVHNPCSNMKLGSGVFRSADIIEKKLKVALGTDGC
                     SSNNNLDMREEMKIASLLAKVHYGSEALKVEEVFRWATLNAARAYGLDAGEIAVGKLA
                     DALIIDLNNVRMVPSYDLLSNWVYSADSSCIDSVICDGKFLMQGGKVPGEELILEAAF
                     KASSRLAAKNNRN"
     gene            947..2146
                     /locus_tag="PKY68_03455"
     CDS             947..2146
                     /locus_tag="PKY68_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOH24126.1"
                     /translation="MPIPSRRGALMPESPIRKLVPLSRQAIARGVKIYHLNIGQPDIK
                     TPQVGLDALKKIDRSILEYTPSEGLLSLRKKLVGYYKKFQIDLDAEDIIITTGGSEAV
                     LFAMMACLDPGDEIIVPEPAYANYTAFAVACGAVIRPFLLNIENGFAMPPAEEFEKMI
                     TPRTRAILISNPNNPTGYLYSRKELEDMRDLVEKYDLYLFSDEVYREFCYTGAPHISC
                     LHLEGIRDNVILIDSVSKRYSECGIRIGALISKNEQVRQNVMKFCQARLCPPLIGQLI
                     AEASLDVHESYMEKVYQEYLGRRNFMIEAINKIHGCYSPMPMGAFYTVARLPIDDADR
                     FCAWLLDSFEYDRKTIMVAPASGFYSAPNVGRNEVRLAYVLNKDDLATAMMLLEKALE
                     IYPGRTI"
     gene            2204..3451
                     /locus_tag="PKY68_03460"
     CDS             2204..3451
                     /locus_tag="PKY68_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH24127.1"
                     /translation="MSLEYFIAKRLFFKTEDQKQVSRPAVRIATLGIATGLAVMLISV
                     SVVVGFKRQVEKNIVGLSSHIQVTSYQSNYSYEMAAMDLPEQLPEQLKSVPGVRHVQR
                     FSTKPGIIKTEDSFQTLVFKGFGPDFDWNFLERYLLEGNLPELNDNLPSTQVLLSAEI
                     ARRLKLTPGDKLLCYFTRDEQLYTRQWELCGIFDTHFAEFDKIFALVDDRQIVRLNAW
                     DSNQAGGLEIFVDDIRELPQVESDVYNLLFQTGNLLGNAYMVQSVYDLNPDLFGWLDL
                     LDMNVWLILVLMIFVSGFNMISGLLILILERTNLIGMLKAMGADNVSVRKVFLYLSSF
                     LIVRGMFWGNVIGLGLCSLQYYFRFLKLDPAVYYIDAVPVEINLGYIVLLNLGTLLAS
                     LLMILGPSALVARITPVKAIRFD"
     gene            3513..4391
                     /locus_tag="PKY68_03465"
     CDS             3513..4391
                     /locus_tag="PKY68_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOH24128.1"
                     /translation="MGKILDLLKKQKYLVSDGAWGTFLQAKGLQAGTCPELWNIDHPE
                     AVKDIARSYLEAGADVVETNSFGGNSCKLEHYGLAGRAYEINRAAAALSRQAAGPDKI
                     VLGSVGPTGKFLMMGELSPEEMFQVFYEQCQALKEGGADAAVVETMTDLEEAVIAVRA
                     AVKTGLEALCTMTFDPLAGGGYRTMMGISPSDMIQPLLEAGAAVIGSNCGNGSRGMID
                     IVREIRSVNTEVPVLIQANAGLPVYKDGKTIFPESPEEMAASVPGLLEAGANIIGGCC
                     GSTPAHIRAIAARIRE"
     gene            4401..4742
                     /locus_tag="PKY68_03470"
     CDS             4401..4742
                     /locus_tag="PKY68_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24129.1"
                     /translation="MKPDFDKIIDRRHTNSLKHDSCDVVFGREDVLPLWVADMDFPVS
                     EAVIGAMKKRLEHPVFGYFTHSEAFYQSIVDWMCRRHAWEIDKDWICFTPGVVPVWLC
                     QCKLLAGPATK"
     gene            4703..5569
                     /locus_tag="PKY68_03475"
     CDS             4703..5569
                     /locus_tag="PKY68_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOH24130.1"
                     /translation="MSVQAFSRPGDKIIVQPPVYHPFYYVVERQGRVLLYNKLIRGED
                     QYFMDFPDLEEKLKSGAKMLLLCNPHNPVGRSWTKEELQRLGELCLKYHCLVVSDEIH
                     ADLIMKGYVHTPMASLSPSIAGNTITFMAPSKTFNLAGMASSEAIISNPVLRKAFFDI
                     SNGALHINMGNTFGDVALEAAYTHGEAWLDALLEYLNKSSDYLTNFISQKLPALRLYR
                     HEATYLIWVDFSALGMEHKALQNKLVHEGGLGFSEGSIFGPGGEQHMRINIACPRSVL
                     ETAMERLQACRF"
     gene            5634..7184
                     /locus_tag="PKY68_03480"
     CDS             5634..7184
                     /locus_tag="PKY68_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOH24131.1"
                     /translation="MKKHLLILTVVFGIAAMTVSAEKANVVVNADQPGAQISKHIYGH
                     FAEHLGNCIYGGIWVGPESSIPNTDGYRNDIITALKQIQIPNLRWPGGCFADEYHWKD
                     GIGPRASRPKMINTHWGGVVEDNSFGTHEFLNFCELIGTEPYICGNVGSGTIEEMSKW
                     VEYITFDGESPMANLRRENGRDKPWKVRFWGVGNENWGCGGNMTPEHYANLYKNYATY
                     CRNYGDNRLFKIAGGANSADYNWTEVMMRNVGAGQMWGLSLHHYTVPKGWGPGGKGSA
                     TDFTEEEYFTTMEKTLFMDELIGRHVAIMDRYDARKRVALCVDEWGAWYDVEPGTNPG
                     FLYQQNTLRDALLCAANMNIFHKYCDRVRMANIAQMVNVLQSIILTKDDKIVLTPTYH
                     IYDMYKVHQDATLIPVEILSPVYKLGNRSLTALSVSASKDTQGVIHISIVNIDPSNDI
                     EASCLLKGVDAKNVDGVILSSGNLTDKNTFEEAQKVVLKEFKKAKINKGELTLTVPAK
                     SVITLAIQ"
     gene            7242..>7852
                     /locus_tag="PKY68_03485"
     CDS             7242..>7852
                     /locus_tag="PKY68_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOH24132.1"
                     /translation="MRTYNFLAFDLGATSGRAILGTLGGGKLETKELTRFPNLIMPIH
                     GKSYWNIFGLYESLKAGLKAAAATGQKIDAIGIDTWGVDFVYVGKDGTFLGCPRAYRD
                     PYTNSIPEKYFKLIPRKEVYKRTGIQIMNFNSLFQLYAAKLEKSSALEAADKILFMPD
                     ALSYMLTGKMVCEYSIASTSQILNLRTRKFDPSLLEVAGINPD"
BASE COUNT         2035 a         1763 c         1967 g         2087 t
ORIGIN      
        1 gggcatccgg gctgccgttg gagttacttt tctggaaagt aacggtgcaa aatatataga
       61 acggggcttg cgcttgctcg aaaatctgcc tgaaacctcg tcgcgtgtgc agatcaccgt
      121 tgctcctcat gctgtctata cggttagtga agacttgttt aaacgtttgg cggctatggc
      181 ccgggaacgt tctctgatta ttcacaccca tttggccgaa acccggcaag aagtacagga
      241 ttgtatagcg gctcacggca tgagccctgt gcgttggctc gatcatttag gcttgttgac
      301 ccccaacacg gtggcggcac acgtcgttca cgcagacgac tatgaattgg atttgctcag
      361 agatagaggc gtttggctgg tgcataatcc ttgttccaac atgaaactgg gctccggtgt
      421 ttttcgcagt gcagacatca tagaaaaaaa attaaaggtg gccctgggta ccgacggttg
      481 ttcttccaat aataacctcg acatgcggga agagatgaaa atcgcctctc ttctggctaa
      541 agtacattac ggcagcgaag cgctgaaggt tgaagaagtt tttcgttggg ctaccttgaa
      601 cgctgcccgg gcttacggcc tggatgccgg cgagatcgcc gtgggtaaat tggccgatgc
      661 tttgattatc gatttgaaca atgtgcgtat ggtgccctcg tacgatttac tttcgaattg
      721 ggtctattcg gccgacagtt cttgcataga cagcgtgatt tgtgacggta agttcctgat
      781 gcagggaggc aaagtgcccg gtgaggaact tattttagag gcagccttta aagcttcctc
      841 caggctggcc gcaaaaaaca accgaaacta aaacaagccg gtctcttgag cagaaatgcc
      901 ggaaacatgc tatatttgca gcttcattta caatagttat acttttatgc cgattccatc
      961 acgccgcgga gctttaatgc ccgaatcacc catccgaaag ttggttcccc taagcaggca
     1021 ggccattgcc aggggagtta aaatatatca tctcaacata ggtcagcccg atataaagac
     1081 cccccaggtg ggcttggatg ccttaaaaaa aattgatcgt tccatcctgg aatatactcc
     1141 cagcgaaggt ttgctgagtt tacgcaagaa acttgtcggt tattataaga agttccaaat
     1201 cgacctcgat gccgaggata tcataattac taccggcggt tccgaagccg tgctttttgc
     1261 catgatggcc tgcctggatc ccggagacga aattattgtt ccggaaccgg cctacgccaa
     1321 ctataccgcc tttgccgtag cttgcggtgc agtaatcagg ccttttctgc tgaatatcga
     1381 aaacggcttt gccatgcctc ccgccgagga atttgaaaaa atgatcaccc cgcgtaccag
     1441 agcgattctg ataagcaacc ccaataatcc caccggttat ctttacagtc gcaaggaact
     1501 ggaggatatg cgcgacctgg tagaaaaata cgatttgtat ttgttttcgg acgaggttta
     1561 tagggaattt tgctataccg gggctcctca tatttcttgt cttcaccttg aaggcatacg
     1621 cgacaatgtg attttaatag actcggtttc gaaacgttac agtgaatgcg gcattcgtat
     1681 aggtgctctt atcagcaaaa atgaacaggt tcgtcaaaac gtgatgaagt tttgccaggc
     1741 ccgtttgtgt ccgcccctca tcggtcagct gatagccgaa gcttctctgg acgttcacga
     1801 aagctacatg gaaaaggtgt accaagaata cctggggcgt agaaatttta tgattgaggc
     1861 cataaacaaa atacacggct gttattcgcc tatgcccatg ggagcctttt ataccgtagc
     1921 ccgtttgccc atcgacgatg cagatcgttt ttgtgcctgg ttgctggata gttttgaata
     1981 cgatcgcaaa accattatgg ttgctccggc ttctgggttc tattcagccc ccaatgtggg
     2041 cagaaacgaa gtgaggctgg cctatgttct gaacaaagac gatctggcca cagccatgat
     2101 gctgctggaa aaggcgttgg aaatataccc cggacgaacc atttgattac ggcgaaaagc
     2161 tagttcctgg gccgtccaag gaattgttca taaaatttgc ctattgagtt tagaatattt
     2221 tatagctaaa cgcttgttct tcaaaaccga agatcaaaag caggtttccc ggccggcggt
     2281 acgcatagct acccttggta ttgcgacagg tttggcagta atgctgattt ccgtgtctgt
     2341 agtggttgga tttaaacgtc aggtggaaaa aaacatcgtc ggcttgagct ctcatattca
     2401 ggttaccagc taccagtcaa actattctta cgaaatggca gccatggact tgcccgaaca
     2461 actgcccgaa caactgaaat ctgtccccgg agtacgtcat gtacagcgct tttctaccaa
     2521 gccgggcatc atcaagacgg aagattcttt tcaaaccctt gtttttaaag gatttggtcc
     2581 cgattttgat tggaacttcc ttgagcgcta cctgctggag ggcaatctac ccgaattaaa
     2641 cgacaacctg ccttcgactc aggtgttgct ctccgccgag attgcccgac gcctgaaatt
     2701 gacccccggc gataaactgc tctgttattt tacccgggac gaacaattat acacccgtca
     2761 atgggaactc tgcggaatct ttgatactca ctttgccgaa tttgataaaa tttttgcttt
     2821 ggtcgacgac aggcaaattg tcaggctgaa tgcctgggac agtaaccagg ccggcggctt
     2881 agaaattttt gtggatgata tcagagaatt gcctcaggta gagagcgatg tgtataattt
     2941 gctgtttcaa acaggcaacc tgttgggcaa tgcttacatg gtacagtcgg tgtatgactt
     3001 gaatcccgac ttgtttggct ggcttgatct gctggatatg aatgtctggt tgatattggt
     3061 gttgatgatt tttgtatcgg gttttaatat gatttccggt ttgctgatcc tgattcttga
     3121 acgtaccaac ttgattggaa tgctcaaagc tatgggcgca gacaacgtct ctgtacgcaa
     3181 ggtctttttg tatctttcgt cctttttgat tgtcagaggt atgttttggg gcaatgtaat
     3241 cggcttgggt ttatgctcgc tacagtatta tttccgtttc ctcaaactgg atccggctgt
     3301 ctactatatt gatgccgtgc ctgtggagat aaatttgggc tatatcgtat tactgaacct
     3361 gggtacttta ttggcttcgt tgctcatgat tttgggacct tcggcgctgg tagccagaat
     3421 taccccggtg aaagccattc gtttcgatta atctgaaatt ttctcgctga ttctgaaatt
     3481 ttcctgtatt tttgtcggta catcgaacac atatgggaaa aatacttgat ttgctcaaaa
     3541 agcaaaaata cctggtctcc gatggagcct ggggaacctt tttgcaggcc aaaggcttgc
     3601 aagccggcac ttgcccggaa ctatggaata ttgaccatcc cgaggcggtg aaggacatcg
     3661 cccgcagtta tttggaggcc ggtgcagatg tggtcgaaac caacagtttt ggtggaaact
     3721 cctgcaaatt ggagcattac ggcctcgccg gccgggcata tgaaatcaat cgtgctgcgg
     3781 cagctctctc caggcaggct gccggtccgg acaaaattgt actgggatcg gtggggccga
     3841 caggcaagtt tctgatgatg ggcgagctaa gtcccgaaga aatgttccag gtgttttacg
     3901 aacaatgtca agctctcaaa gaaggaggag ccgatgccgc agtggtagaa accatgactg
     3961 atctggaaga agccgtaatc gctgttcgtg ctgctgtaaa aacaggcctt gaagcgcttt
     4021 gcaccatgac cttcgatcca ctggcgggag gcggctacag aaccatgatg ggtatttcac
     4081 cttccgatat gattcaaccc ctgttagaag cgggggccgc ggtgataggc agcaattgcg
     4141 gtaacggttc caggggaatg atagatatag tacgcgaaat ccgcagtgtg aatactgagg
     4201 ttccggtatt gatccaggcc aacgccggat tgccggttta taaagacggg aaaaccattt
     4261 ttcccgaatc tcccgaagaa atggcggcct ccgttcccgg attactggaa gccggagcca
     4321 atattattgg gggatgttgc ggtagtaccc cggcccatat cagagctatt gctgcccgga
     4381 tcagagaata aatcgtgaaa atgaaacctg atttcgacaa aattatcgac cggcgccaca
     4441 ccaatagtct gaaacacgac tcctgtgatg tggtctttgg ccgtgaagac gtattgccgc
     4501 tttgggtggc cgatatggat ttccctgttt ccgaagcagt gataggggcc atgaagaaac
     4561 gactggaaca tcctgttttt ggttatttta ctcacagcga ggctttttat cagtccattg
     4621 tggattggat gtgcaggcgt cacgcctggg agatcgataa agattggatt tgtttcactc
     4681 cgggggtggt tccggtttgg ctatgtcagt gcaagctttt agccggcccg gcgacaaaat
     4741 aattgtacag cctcccgttt atcatccctt ttattacgtg gtcgagagac agggccgggt
     4801 tttgctctac aataagctga tccgcggtga ggaccaatac ttcatggatt ttcccgattt
     4861 ggaggaaaaa ctgaagtcag gggccaagat gttgttgttg tgcaatcccc ataatccggt
     4921 cggccgaagc tggacaaaag aagaattgca aaggctgggc gaattatgtc tgaaatatca
     4981 ttgcctggtg gtatccgacg agatacatgc cgatttgata atgaaaggct acgtacacac
     5041 gcctatggcc agtttgagcc cttcaatagc cggcaatact attactttta tggctcccag
     5101 caaaactttc aatctggccg gtatggccag ttccgaagct ataatttcga atcccgtgct
     5161 gagaaaagcc tttttcgata taagtaatgg ggctttgcat atcaatatgg gtaatacttt
     5221 tggagacgta gccctggagg ctgcctatac ccatggcgaa gcttggctag atgctttgct
     5281 ggagtatcta aacaaaagtt cagattattt aaccaacttt atttcgcaaa aattgccggc
     5341 tctgaggctt tatcgtcatg aggctaccta tttgatctgg gtggattttt ctgcattggg
     5401 catggagcat aaagccttac aaaataagct ggtgcacgag ggcggattgg gttttagcga
     5461 aggcagtatt tttggcccgg gaggggagca acatatgcga attaacattg cttgtccgcg
     5521 ttctgtgttg gaaacagcta tggagcgact acaggcttgt cgcttttaaa gaaaaaaaat
     5581 tatctttgct gaatgcaaaa ctttttatca acaaattgaa taattctatc gctatgaaaa
     5641 aacaccttct tatcctgaca gttgtcttcg gtattgccgc gatgactgta tctgcagaaa
     5701 aggccaatgt ggttgtaaac gccgaccaac cgggtgctca aatcagcaag catatttacg
     5761 gacattttgc tgaacatttg ggtaattgta tttacggtgg catctgggtg ggtccggaat
     5821 cgtctatccc caacaccgat ggttatcgca acgacataat tacggctctc aaacaaattc
     5881 agatacccaa cctgcgttgg cccgggggtt gctttgccga tgagtaccac tggaaagatg
     5941 gtatcggacc cagagcaagc cggcccaaaa tgatcaatac ccactggggc ggagtggtcg
     6001 aagacaactc ttttggtacc catgaatttt tgaatttttg cgaattgata ggtaccgagc
     6061 cttacatttg cggtaatgtg ggcagcggaa caatcgaaga aatgtccaag tgggtggaat
     6121 acatcacttt cgacggagaa tcgcccatgg ctaacctgag gcgcgagaat ggacgcgaca
     6181 aaccctggaa agtgagattc tggggtgtcg gcaacgaaaa ctggggttgc ggaggaaata
     6241 tgacccctga acactacgcg aacttataca agaattacgc cacttactgt cgtaattacg
     6301 gagacaaccg cttgttcaag attgccggcg gagccaattc ggccgattac aattggaccg
     6361 aagtgatgat gagaaacgtc ggcgccggtc aaatgtgggg cctttcatta catcattaca
     6421 ctgtccccaa aggctggggt cccggaggta agggatctgc caccgatttt accgaagagg
     6481 aatacttcac caccatggag aagacgttgt ttatggatga gctgatcggc agacacgtgg
     6541 ccataatgga caggtacgat gcccgcaaaa gagttgccct ttgtgtagac gaatggggag
     6601 cctggtacga tgtagaaccc ggcaccaatc ccggcttcct ctatcagcaa aacaccctgc
     6661 gcgatgcttt gctgtgtgcg gccaacatga atatttttca caaatactgt gatcgtgtcc
     6721 gcatggctaa cattgcacaa atggtgaatg tcttgcagtc cattattctg accaaagacg
     6781 ataagatcgt gctgacacct acctatcata tttacgatat gtacaaagtg catcaggacg
     6841 ccactttgat tcccgtcgaa atactgagcc cggtgtacaa gctggggaac agatctttga
     6901 cggctttgag cgtcagtgct tcgaaagata cccagggtgt gatacatatc tcgatagtca
     6961 acattgatcc cagcaacgat attgaagcct cttgcctatt gaagggagtg gatgccaaaa
     7021 acgtggacgg agtaatattg agttccggta atcttacgga caaaaatacc tttgaagagg
     7081 ctcagaaagt cgtacttaag gagttcaaaa aggccaaaat aaacaaggga gaattgactc
     7141 tgacggttcc cgccaaatct gtaataactc tggccattca ataatcggcc attcaataaa
     7201 taatgtaacg gggtgtgagg acatcccaaa taagctacaa gatgcggaca tacaattttt
     7261 tagcattcga tctgggagcc accagcgggc gagccatttt aggaacgctg ggcggaggta
     7321 aactcgaaac gaaagaatta acccggttcc ccaatcttat tatgcctata cacggcaagt
     7381 cgtactggaa cattttcgga ctctatgagt ctctcaaagc gggattaaaa gctgccgccg
     7441 caaccggaca aaaaatcgat gccattggta tcgacacctg gggcgtagat tttgtctatg
     7501 tcggcaaaga cggtactttc ctgggttgtc cccgggccta tcgcgatcct tacaccaatt
     7561 ccattcccga aaagtatttt aaactgattc cccgtaaaga agtatacaaa cggacgggca
     7621 ttcagattat gaattttaac agtttgtttc aactttatgc tgccaaactg gagaaatcat
     7681 ctgccctgga ggcagccgac aagattttgt ttatgcccga tgctctttcg tatatgttga
     7741 ccggaaaaat ggtttgcgaa tacagcattg cctccacttc gcagatactg aatctaagaa
     7801 ccagaaagtt tgatccttct ctgctcgagg ttgccggtat caatccggac at
//
LOCUS       DAOSGL010000069         7847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_7999_length_7847_cov_3.736525, whole genome shotgun sequence.
ACCESSION   DAOSGL010000069 DAOSGL010000000
VERSION     DAOSGL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7999_length_7847_cov_3.736525"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKY68_03490"
     CDS             <1..560
                     /locus_tag="PKY68_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOH24133.1"
                     /translation="LGRATHIENFGTVLCAGGGVGIAPLLPIVEGMKKAGNKVISVLA
                     ARSKNLIMLEDEIRRHSDEVVIMTDDGSYGEKGLITVGVEKIIQREKVDLCVTIGPAV
                     MMKYVSLLTKKYEVPTVASLNTIMVDGTGMCGGCRVSVGGKTKFVCVDGPEFDAHQVN
                     FDEMIMRLGTYKEEEQEEMKKLHQK"
     gene            578..2050
                     /gene="gltA"
                     /locus_tag="PKY68_03495"
     CDS             578..2050
                     /gene="gltA"
                     /locus_tag="PKY68_03495"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOH24134.1"
                     /translation="MTKEEQIAALRAQTWREDLRKSRKTKERTDIPRVEMNEQDAEVR
                     SHNHLEVNLGLTAEQARQEAARCLDCANPTCILGCPVEIQIPSFVKNIERGDFLEAAR
                     VLKETSALPAVCGRVCPQEKQCEAQCIYTLKLGKPSVAIGHLERFAADYERESGRISV
                     PELAPKNGKKVAVVGSGPAGLSFAGDMAKYGYEVMVFEALHEIGGVLKYGIPEFRLPN
                     SVVDVEIDNLRQMGVQFMTNCIVGRTITIDELKEQGYCGIFVGSGAGLPNFMNIPGEN
                     LIHILSSNEYLTRVNLMDAANPESDTPVAMGKCVAVIGGGNTAMDSVRTAKRLGAERA
                     IIVYRRSEEEMPARVEEVKHAKEEGIEFLTLHNPLEYYGDERGRVKQMRLQKMELGQP
                     DDSGRRRPVPIPGAEVVIDVDQVIVSVGVSPNPLIPSSVEGLEISRWGTIVVDDNMRS
                     SIPAIYAGGDIVRGGATVILAMGDGRKAAASMHAQLSGKE"
     gene            complement(2122..2526)
                     /locus_tag="PKY68_03500"
     CDS             complement(2122..2526)
                     /locus_tag="PKY68_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOH24135.1"
                     /translation="MEQKGFTEVLDKFRQQIIDEEFDQIVAIANGGIIPAAILAQKLE
                     LEVFLLKINLRDKQQRPLYDQPKLLAEIDFDCKDKRILLVEDRVKTGSTLNFAKQLLN
                     EAGAAKVKSFAVNGKADYSLYDENCFRFPWII"
     gene            complement(2533..3849)
                     /locus_tag="PKY68_03505"
     CDS             complement(2533..3849)
                     /locus_tag="PKY68_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOH24136.1"
                     /translation="MAKSLKHPKGLYLLFMTEMWERFSYYGMRALLVLYLTQSVLEGG
                     MGFSEENATLLYGFFTGLVYMTPIIGGWLADRYLGQRRAITIGGITMMLGQFCLASTN
                     SVTMLYLGLFLLIIGNRFFKPNISVLVGDLYEPGDKRRDSAFTIFYMGINIGAGLSPL
                     VCGWLGMLYGYRAGFLAAGIGMLIGQLIFNLIGPKYLGNLGSVAVGNKNHPNAQNHPL
                     TKEEKDRTWVIIIIVLFCVFFWAGFEQAGSSLTLYTEKYINRNVFGFEIPTAWFQSVN
                     SFFIVLLAPVFSMLWTFLATRNKEPGVPLKMGLGMIMLGVGFLLMVMACLQRGGDNPD
                     ETVKANLLFLVGTYLFHTFGELLLSPIGLSMVTRLAPVKLASLLMGVWFLSSFVANII
                     GGFVASYALRMGAMSIFITIAAISVMLGLLLISMNKWLFKKSHGTL"
     gene            4245..6680
                     /gene="thrA"
                     /locus_tag="PKY68_03510"
     CDS             4245..6680
                     /gene="thrA"
                     /locus_tag="PKY68_03510"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOH24137.1"
                     /translation="MKVIKFGGTSVGSVQALLQLKRIVEKQAEKPVVIVVSAVAGMTD
                     FLIKTARTAAEGDSNYLNYLAEMKKKHFSLVEGAFAPEQQKKLTEELSGIFDELGNIF
                     QGVALIRELSDKSEDVIVSYGERLSSLMVSRLLDIPLYDARKLIKTNHKFGKHVVNFE
                     ETSAAIKTQLNRLPELSVIGGFISSDAESGEITNIGRGGSDYTAAILASELNASILEI
                     WTDVDGFMTADPKVINNAYVIENLSFVEAMELSNFGAKVIYPPTIFPVYHKNVPIRIK
                     NTFNPEAPGTYISRDKVLGENKAIKGISSIPDTSLITIQGLGMVGVIGVNYRIFKTLA
                     KAGISVFLVSQASSENTTSIGVRNSDAEITVRVLREEFDHEIEMGEINDIFAESNLAT
                     VAIVGEGMKRTPGIAGKLFTTLGRNGINVIACAQGASETNISFVIKLDHLRKSLNVIH
                     DSFFLSEYQVLNLFVIGIGTVGGSLLEQIKRQQPKLMAENGLKLNVVGIADIEKAVFS
                     REGIDLEHYKELLKREGKTSSPELLRNEILGMNIFNSVFVDCTASSEISALYADLLSH
                     NVSVVAANKIAASSDYDNYARLKELARRKGVKYLFETNVGAGLPIINTMNSLIHSGDR
                     ILKMEAVLSGTLNFIFNTISAENPLSKTIKMAQEKRFSEPDPRIDLSGTDVIRKLVIL
                     AREAGYRVEQEDVVKNLFIPDKYFKGSLEDFWKNIPEMDADFEAKRKLLEKDSLRWRF
                     VAKMENGACSVGLHEVGPSHPFYDLEGSNNIILIQTERYFQDPMVIKGYGAGAGVTAA
                     GVFGDIISIANIR"
     gene            6682..>7847
                     /locus_tag="PKY68_03515"
     CDS             6682..>7847
                     /locus_tag="PKY68_03515"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOH24138.1"
                     /translation="MKYLIVLGDGMADEAMETLGGKSPMQVAFTPCMDRLAREGRCGR
                     LVTVPEGFPPGSEIANLSLLGYDVSKQFEGRGSLEAASMGIDVPEGYMAARCNLICIQ
                     DGKIKNHSAGHISTSEARELIDFLNKELGNEDLVFHTGVSYRHLLLLKGGNKELDCTP
                     PHDVSGTAFAEVLPRAVHPRAEQTARLLRELILRSQEILPAHPVNLARKARGLDTANS
                     IWPWSPGYRPAMLPLREKYPIRSGSVISAVDLIKGTGVYAGLEPIEVPGATGLYDTNY
                     EGKAQAAIDELKKKDFVFLHVEASDEASHEGNVDLKIRTIEYLDQRLIRPILEEISTW
                     DEAVTIAVLPDHPTYCRTKTHAAEAVPFLIYRSDAGSAQARPTKGPDSFFSPGPG"
BASE COUNT         2103 a         1836 c         2040 g         1868 t
ORIGIN      
        1 ctttgggcag ggctactcat atagaaaatt tcggcacggt gctctgtgcc ggcggaggag
       61 tagggattgc ccccttgctg cccatagtcg aaggcatgaa aaaagccggt aacaaagtga
      121 tctcggtgct ggcagcccgt tccaaaaacc tgattatgct tgaagacgag attcgccggc
      181 attccgacga agtcgtcatc atgaccgacg acggttctta cggagaaaag ggcttgatta
      241 cggttggggt agaaaagatt attcaacgcg aaaaggttga tctttgtgta accatcggtc
      301 ctgccgtgat gatgaagtat gtgtcgctgc tgaccaagaa atacgaggtg cccaccgttg
      361 cttcgctcaa cactattatg gtagacggca ccggcatgtg cggaggttgc cgtgtgagcg
      421 tgggcggaaa aaccaaattt gtttgtgtgg acggccccga atttgacgct catcaggtga
      481 attttgacga aatgattatg cgcctgggta cttataaaga agaagaacag gaagaaatga
      541 aaaaacttca tcagaaataa caagggaaga atcagcaatg acaaaagaag aacaaatcgc
      601 ggctctgcgc gctcaaacct ggcgcgagga cttacgtaaa agccgtaaaa ccaaggaaag
      661 aaccgatatc ccccgggtag aaatgaacga gcaggacgca gaggtccgca gccacaatca
      721 tctggaagtc aatctgggtt taacagccga acaagccaga caggaggctg cccgctgtct
      781 ggattgcgcc aatcctacct gtattttggg ttgtccggtg gaaattcaga ttccaagctt
      841 tgtaaaaaac atcgaacgcg gcgactttct tgaagcggca cgagtgctga aagaaaccag
      901 tgcgctgccg gcagtttgcg ggcgggtctg tcctcaggaa aaacaatgcg aagcccagtg
      961 catctacacg ctcaagctgg gtaagccctc ggtggctata ggtcatctgg agcgttttgc
     1021 cgccgattac gaacgcgaga gtggccgtat ttcggtgccg gaactggcgc ccaaaaacgg
     1081 taaaaaagtg gctgtggtgg gttcgggacc tgccgggctt tcttttgccg gcgacatggc
     1141 caaatacggt tatgaagtaa tggtgttcga agccctgcac gaaataggcg gggtgctcaa
     1201 atacggtatt cccgaatttc gtctgcccaa ttccgtggtg gatgtggaaa tcgataatct
     1261 gcgtcagatg ggcgtgcaat tcatgaccaa ctgcatcgta ggcaggacta tcaccataga
     1321 cgaactcaaa gaacagggtt attgcggtat tttcgtagga agcggagccg gtttgcccaa
     1381 ttttatgaat attcccgggg agaatcttat tcatattctc tcgtccaacg aataccttac
     1441 ccgggtcaat ctgatggatg ccgccaatcc cgaatccgat acgccggtgg ctatgggcaa
     1501 gtgcgttgcc gtgataggcg gtggtaatac cgccatggac tccgtgcgta cggccaagcg
     1561 tctgggagcg gagcgggcta ttatagtgta ccgccgttcc gaagaagaaa tgcccgcccg
     1621 tgtcgaggaa gtgaaacatg ccaaagaaga gggcatagag ttccttacct tgcacaatcc
     1681 tctggaatac tacggcgacg aaagaggccg ggtaaaacag atgcgtctgc aaaaaatgga
     1741 actgggtcag cctgacgatt cggggcggcg acgcccagtg cccattccgg gtgccgaagt
     1801 agtgatcgat gtcgatcagg tcattgtcag cgtgggtgtt tcacctaatc ctttgatccc
     1861 tagctcggta gaaggcctgg aaataagccg ctggggcacc atagtggtag acgacaacat
     1921 gcgttcgtct attcctgcca tctacgccgg gggcgatatc gtgcgcggcg gagccacggt
     1981 gattctggcc atgggcgacg gccgcaaggc tgcagcctcg atgcacgcac agctttccgg
     2041 aaaggaatag ctcctgaagg aatagcctct tacagcgaac ctcggatagc gggcgttaca
     2101 gttgcttccg gattctgatt gttaaataat ccaggggaag cgaaaacaat tttcgtcgta
     2161 gagtgagtaa tcggctttgc cattcacggc aaagcttttt actttggcgg ctccggcttc
     2221 gttaagcaat tgtttggcaa aattcagggt actgcccgtc tttacccggt cttcgaccag
     2281 aaggatgcgt ttgtctttac aatcaaagtc gatttcggcc agtaacttgg gttggtcgta
     2341 aagaggtcgt tgctgtttgt ctctgaggtt gatcttgagc agaaacactt ccagttcgag
     2401 cttttgagcc agaatagcag cgggaataat gcccccgttg gcgatggcca ctatttggtc
     2461 aaattcttca tcaataatct gttggcggaa tttgtccagt acttccgtaa agcctttttg
     2521 ttccatgatg tattacaaag taccgtgcga tttcttgaac aaccacttat tcatggaaat
     2581 cagcagcagg ccgagcataa ccgatatggc cgcgatggtg ataaagatac tcatcgcacc
     2641 catcctcaaa gcgtacgaag cgacaaaacc tcctataata ttagctacga aactgctcag
     2701 gaaccaaaca cccatcagca gcgaggctag cttaaccgga gccagtctgg ttaccataga
     2761 gagtccgatg ggcgacagga gcaattcacc aaaagtgtgg aacaagtagg ttccaaccaa
     2821 aaaaagcaga ttggctttga cagtttcgtc gggattgtcg ccgccgcgtt gcaggcaggc
     2881 cataaccatc agcagaaagc cgacgccgag cataatcatg ccaaggccca ttttcagagg
     2941 aactccaggc tccttgttac gggtggccaa aaaggtccag agcatcgaaa aaacaggggc
     3001 gagcaataca ataaagaaag aattcaccga ctggaaccag gctgtgggaa tctcgaatcc
     3061 gaacacattg cggttgatgt atttctcggt gtacaaagtc agggaactac cggcctgttc
     3121 aaagccggcc cagaagaaca cgcaaaacag tacgataatg ataatcaccc aggtgcggtc
     3181 tttttcttct ttggtcagag gatgattctg ggcattggga tggtttttgt tacctacggc
     3241 gacactacct aaattaccca ggtatttggg gccgataagg ttaaaaatca attgccctat
     3301 aagcataccg ataccggcag ccagaaaacc tgctctgtaa ccgtatagca ttcccagcca
     3361 gccgcaaacc aaaggcgaaa gcccggcgcc gatgttgata cccatataga aaatggtaaa
     3421 ggccgaatct cttcgtttgt cgcccggttc ataaagatcg cctaccagca ccgatatatt
     3481 gggtttgaaa aagcggtttc ctataatcaa cagaaacaaa cccaggtaaa gcatggttac
     3541 actattggta gaggccagac aaaattgtcc caacatcatg gtaataccgc ctatggttat
     3601 ggcacggcgt tgtcccaggt atctgtcggc cagccagccc cctatgatag gggtcatgta
     3661 gaccaggccg gtaaaaaagc cgtagagcag agtggcgttt tcttcggaaa aacccatacc
     3721 tccttcaagc acagactggg ttaaatacaa tacgagcaga gctcgcatac cataataact
     3781 gaaacgttcc cacatttcag tcataaaaag cagatataaa cctttggggt gtttaagtga
     3841 tttagccatt aataatgcag aattagacgt ccggacaggc ctggggctta tcaaacggag
     3901 gtcttaagtt tcaataagcc gcaaaaataa attaattgga cgaaataaac ggtttttttt
     3961 tgtttttgtt tctaaataat ctatatttgc gcactcaaaa gggcttgcag ccccagttcg
     4021 aaagagatga tgttttggca actacatata accaccacta ctaccacaac ccctgcgggg
     4081 gtctgattat ctttcgcatt ccaatcgcga aggccggggg ccttttcgat tttccaacaa
     4141 acaacaatta tttttttagg gttctaattt ttaaaaattc atgcatagtt tatatggcat
     4201 gatttaaggt aatcgtatgt gtcacaatat caatatagag caaaatgaaa gttattaaat
     4261 tcgggggaac ctctgtgggt tccgtccagg cgctgcttca gttgaagcgt attgtagaga
     4321 aacaagccga aaagccggta gtaatagtag tatccgccgt agccggaatg accgatttcc
     4381 tgattaaaac agcccgaact gcggccgaag gagacagcaa ctacctgaac tatctggccg
     4441 agatgaaaaa aaagcatttc tctctggtcg aaggcgcttt tgctccggag cagcaaaaaa
     4501 agcttacgga agaactttcg ggtatattcg atgaattagg caacattttt caaggagtag
     4561 ctctgatacg cgaactttcg gacaagtcgg aagatgtgat tgtgagttac ggcgagaggc
     4621 tttcctcgct catggtaagc cgtctgttgg atattccgct ttacgacgcc cgcaagctga
     4681 tcaaaaccaa tcataaattt ggcaagcatg tggttaactt tgaagagacc tcggcggcca
     4741 ttaagacgca actgaaccgg cttcccgagc tttcggtgat aggcggtttt atttcgagcg
     4801 acgccgaaag cggcgaaatc accaatatag gacggggcgg ttccgattat acggcggcga
     4861 ttctggcctc ggaactcaat gcttctatcc tggaaatctg gaccgacgtg gacggtttta
     4921 tgacggccga ccccaaagtg atcaacaacg catatgtcat cgaaaatcta agcttcgtag
     4981 aagctatgga gcttagtaat ttcggagcga aagtgattta tccgcctacc atttttcccg
     5041 tttaccacaa aaacgtaccc atccgcatca agaatacttt caatcccgaa gctccgggca
     5101 cttatatctc gcgcgacaaa gttttgggcg aaaataaggc cattaagggt atttcttcca
     5161 ttcccgatac cagtctgatt accatacagg gcttgggcat ggtgggagtg ataggcgtga
     5221 attaccggat ttttaaaact ttggctaagg ccggtatttc cgttttcctg gtttcgcagg
     5281 cttcgtcgga aaacaccaca tcgataggcg tacgcaattc agacgccgaa ataacggtca
     5341 gggtgctcag agaagaattc gaccacgaaa ttgaaatggg tgaaatcaac gatatttttg
     5401 ccgaaagcaa tctggctacg gtagccatcg taggtgaagg gatgaaacgt acgccgggta
     5461 tcgccggcaa attgtttacg accctgggac gtaacggtat taacgtgatt gcctgtgcgc
     5521 agggagcttc cgaaaccaat atttcttttg tgattaagct cgatcatctg cgtaaaagcc
     5581 tcaatgtgat tcacgattct tttttcctga gcgaatacca ggtgttgaat ctttttgtga
     5641 tcggtatcgg tactgtgggc ggtagcctgc tggaacaaat caagcgtcag caacccaaac
     5701 tgatggccga aaacggctta aaactcaatg tggtgggtat agccgatatt gagaaagctg
     5761 tgttttcgag agaaggcata gatctggaac attacaagga gttattaaaa cgcgaaggta
     5821 aaaccagtag tccggagttg cttaggaacg aaattctggg tatgaacatc tttaattccg
     5881 tttttgtgga ttgtacggcc agctccgaaa tatcagctct ttacgcggat ttgttgtcac
     5941 acaacgtgtc tgtggtagca gccaacaaaa tagcggcatc gtccgattac gacaattacg
     6001 cacgactcaa ggaattggcc cgtcgcaagg gtgtgaaata cctcttcgaa accaatgtgg
     6061 gagccggcct gcccattata aatacgatga atagcttgat tcacagcgga gacagaattt
     6121 taaaaatgga agctgttttg tcgggtacgt tgaattttat tttcaacacc atcagtgccg
     6181 aaaatcccct gagcaaaacc ataaaaatgg ctcaggagaa acgtttttcg gagcccgatc
     6241 cccgcatcga cctgagcgga accgacgtca tccggaaatt ggtgattctg gcacgcgaag
     6301 ccggttacag agtagaacag gaagatgtgg tgaaaaatct ctttattccc gacaaatact
     6361 tcaaaggcag tctggaagat ttttggaaaa acattcccga gatggatgcc gatttcgaag
     6421 caaaacgcaa gttgctcgag aaagattctc tgcgctggcg ctttgtggcc aaaatggaaa
     6481 acggagcctg ttcggtcggc cttcatgaag tcggtccatc gcatcccttt tacgacctgg
     6541 aaggcagcaa caacatcatt ctgatacaga ccgagcgcta ttttcaggat cctatggtta
     6601 tcaaaggcta cggagccgga gccggtgtaa cggctgccgg ggtgtttggc gacataatct
     6661 ccatcgcgaa cattcgttaa gatgaaatac ctgattgttc tgggagacgg catggccgac
     6721 gaagccatgg agaccttggg cggaaagtcg cccatgcagg ttgcctttac tccctgtatg
     6781 gaccggctgg ccagggaggg tcgttgtggc cggctcgtta cagtgcccga aggttttcct
     6841 cccggcagtg aaattgccaa cctgagttta ctgggatacg atgtaagcaa gcaattcgaa
     6901 ggcaggggtt cgctcgaagc cgccagtatg ggaatcgatg tccccgaagg ttatatggcg
     6961 gcgcgctgca atctgatctg catacaggac ggcaagatta aaaatcattc tgccggccat
     7021 atttccacct ccgaagcccg ggaattgata gattttctga ataaggagtt aggcaacgag
     7081 gacctggttt ttcatacggg tgtttcctat cgtcatctct tgctgctcaa gggtggcaac
     7141 aaagaattgg attgtactcc gcctcacgat gtgtcgggta ccgcctttgc cgaagtgttg
     7201 ccacgggccg tgcatccccg ggcagaacaa acagcccgtt tacttcgcga attgattttg
     7261 cgatcgcagg agattttgcc ggctcatccg gtcaatttgg cccgcaaggc cagaggcctg
     7321 gatacggcta attccatttg gccctggtcg ccaggctacc gtcccgctat gctgccgttg
     7381 agggaaaagt atccgatacg aagcggttcg gtgatttcgg ctgtggattt gatcaagggc
     7441 acaggcgttt atgcaggcct ggaacccatc gaagtgcccg gagccaccgg tttgtacgac
     7501 accaattacg aaggcaaggc tcaggcggcc atcgacgaac tcaagaaaaa agattttgtg
     7561 tttttgcatg tggaagcttc tgacgaagcc agtcacgagg ggaacgtcga tttaaagatc
     7621 cgcaccatag agtacctcga tcaacgcctg attcgcccca tactggagga gatttccact
     7681 tgggacgagg ccgtcaccat agctgttttg cccgaccatc ccacctactg ccggaccaaa
     7741 acccatgcgg cagaggcggt tccctttttg atttatcgca gcgatgcagg ttctgcgcaa
     7801 gcccggccca ccaaaggccc ggacagcttt tttagcccgg ggcccgg
//
LOCUS       DAOSGL010000070         7825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8039_length_7825_cov_4.214801, whole genome shotgun sequence.
ACCESSION   DAOSGL010000070 DAOSGL010000000
VERSION     DAOSGL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8039_length_7825_cov_4.214801"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1039)
                     /locus_tag="PKY68_03520"
                     /pseudo
     CDS             complement(32..1039)
                     /locus_tag="PKY68_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
     gene            complement(1039..1353)
                     /gene="trxA"
                     /locus_tag="PKY68_03525"
     CDS             complement(1039..1353)
                     /gene="trxA"
                     /locus_tag="PKY68_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH24139.1"
                     /translation="MALEITDSNFEEYLSSGKALVVDFWAPWCGPCRMVGPIIEELAD
                     EYADRVNIGKMNVDENNDVCVRFGIRNIPTILFFKQGQLVNKQVGAIQKSALAQKVEA
                     LL"
     gene            complement(1446..5000)
                     /gene="dnaE"
                     /locus_tag="PKY68_03530"
     CDS             complement(1446..5000)
                     /gene="dnaE"
                     /locus_tag="PKY68_03530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOH24140.1"
                     /translation="MSSFVHLHVHSQYSILDGQASIQDLVDKAVANKMPAIALTDHGC
                     MFGVKEFHNYVKAKNAQSKTPIKPIIGCEVYVARNGKEMHRKEVKEDARGWHLILLAK
                     NFKGYKNLIKIVSRAWTDGYYYCARTDHRDLETYREGLIACSACLGGEIPSYILNDNL
                     EQAEQSVRWFKKVFGDDFYLELQRHKPTVANAAQDTYPKQQLVNKHLIELAQKYQVKL
                     IASNDVHFVDEDHAEAHHRLICLNTGNDVDDYSKMAYTKQEWFKTVEEMQAVFHDLPQ
                     VLENTLEVAEKVEYYDINHEPIMPDFPKPEGFDDDNEYLRFLTYKGAEKVYPDMNEEV
                     RERLEFELEVIKNMGYPGYFLIVQDFTTAARNMGVSVGPGRGSAAGSAVAYCLGITKV
                     DPIKYDLLFERFLNPERISLPDIDIDFDDDGRNAVLRWVEERYGKEKVAHIITYGTMA
                     SKSAIKDVARVQKLPLSESDRLSKLVPDKITDPADSQRRLKVNLKNAIAYVPELKEAA
                     ESDDPALRDTMKYAQQLEGTVRNTGVHACGVIISRDDISDWVPVSTAEDKETGEKLLV
                     TQFDGSVIEDTGLIKMDFLGLKTLSIIKDAVENIRLSRGITIDIDNISLEDEKTYELY
                     SEGKTVGTFQFESAGMQKYLRDLKPSKFEDLIAMNALYRPGPMDYIPQFIARKHGSEA
                     IEYDLPVMERYLKDTYGITVYQEQVMLLSRLLAGFSRGESDELRKAMGKKIREQLDKL
                     RPKFIEGGKKNGYDEGILDKIWADWEKFASYAFNKSHATCYSLVAYQTAWLKANYPSE
                     YMAAVLSRSVNNISDITKFMDECRSMGILVLGPDINESNYQFTVNKQGNIRFGLGAIK
                     GMGEGAVKAIVEERANKPYDSVFDLVERVNLNACTRKNIEVMALAGAFDSLGEIQREQ
                     FFAENDKGEVFLDVLMRYGNRIQLDRASNQNTLFGGEMEVAITRPDIPRAESWSELER
                     LNREKEYVGMYLSSHPLDEYSVFMKYVCNTTIEDIKLSKLELNKEVQFAGIVTEVREK
                     RKQNGDPYAFVTIEDYTASVELPFWSREYVDFGKYLKKDMFLAFRGVYQPRPWKASEN
                     ELKINSISLLSEAAGNAVNKIQITIPLHLISNEFTRRFLSMINKKPGKSLLYITVYDP
                     DNQPKLHASLFSRNYRIQVDKEITDFLEESGEIEIKMN"
     gene            complement(5160..5648)
                     /locus_tag="PKY68_03535"
     CDS             complement(5160..5648)
                     /locus_tag="PKY68_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24141.1"
                     /translation="MKKTLTSVLLASLLAMFVNFNANALGSPLEKGTIAATAALGFQP
                     GLGASLSGEYVLVDSWWEGHFAVGLGIQTVAISSEGLNFGILPKATYGIFLTDAIEVH
                     AGIQLGFGHNKNRGNGFAYGGLLGGRYFFNDTMAAGLELNYTGWGAAYTPLVSLGYTI
                     LF"
     gene            5907..6611
                     /locus_tag="PKY68_03540"
     CDS             5907..6611
                     /locus_tag="PKY68_03540"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOH24142.1"
                     /translation="MKIPFRIYIHKEGWKLLSSVALGLITINLLLFFLAGGITFSISL
                     VISSVLFLFFTNFFRSPRRFYQQSHPGDIVSPADGKVVVVEPVYENLLLKEKRLQVSI
                     FMSVTDVHANWYPLAGKVIHYSHRNGNYHAAWLPKASSENERSSVIIRAAYNQQTVLV
                     RQIAGALARRIVTYAHTGKRCKLNEHLGFIKFGSRVDLFFPLDSVDMFVEQGARVKGN
                     KTVIARFKDKERNETV"
     gene            6598..7302
                     /gene="pssA"
                     /locus_tag="PKY68_03545"
     CDS             6598..7302
                     /gene="pssA"
                     /locus_tag="PKY68_03545"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOH24143.1"
                     /translation="MRLFKYLPNAITLLNALAGCLSIVFAFEKAYTAAAALIALAAVF
                     DFLDGLAARLFQAYSPLGKELDSLADVISFGLAPAMMVFNYYRDLTPGLLSENTIHIL
                     AYSAFILPLFSALRLAKFNIDERQSRGFIGLPTPANALFWAFGVAAIQEYNLPVLSLA
                     IPILILLFSWLLVSPIPMFSLKFSRFTISGNRLRYFFLSGCIVLVLLIGWQAIALCVV
                     WYILLSLFTKADKHLQ"
     gene            7359..7622
                     /locus_tag="PKY68_03550"
     CDS             7359..7622
                     /locus_tag="PKY68_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24144.1"
                     /translation="MKKTKDPSKKRKKKGGRKKVLPKSKPRLRNKRIVFALNEPEYNA
                     LLSYCEKYNISNRSHLIRTVLMTTILKRFNKDYPTLFHEEEMH"
     gene            7623..>7825
                     /locus_tag="PKY68_03555"
     CDS             7623..>7825
                     /locus_tag="PKY68_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOH24145.1"
                     /translation="MSLHQKYMRLALNEALLAYEQNEVPVGAVVVCKGRVIARGHNLT
                     ETLNDVTAHAEMQAIKAAASTLGG"
BASE COUNT         1972 a         1848 c         1744 g         2261 t
ORIGIN      
        1 agaaatataa gttcagctac cataaaatca acaatttttc gccggccgaa ttatcgttga
       61 ttttatggta gctgaactta tatttcttgc ggtagcgttc cagttgggat tgaatcaggg
      121 gcgcttcgtt gaggttttta ccctcttcat cgtcttcgtc tacccatagc tccggaaaca
      181 tttgctgaat ctgcaaagga taaagccccg aaaaaacggc gttccgggcg tattgagtag
      241 cagtaggtaa aatactgaaa tacagctgtt catccatact gaaccactgc cctatttgag
      301 gtttgagcat tctccactga tcgaaacgga aattatcgat caatatgaac cagaccttct
      361 cgcccgcgtc gagcagggga aagacttttt cgcgaaacaa atcgggactc atcaaaggac
      421 gatccggaga accttccatc caacgggcat aatggcggga cacaaaacgg gcaaaaagcc
      481 ggttggcttc gtttttttgc atcagcagga cttcgtccag ggcgtttttg gttccggcca
      541 gctccagttc ccaaaacacc aattttttgt aaacttcttt ccagtcttct tcgcccgaag
      601 cctcttctat ttgcagggca atttccctaa aacgctgctg gtaatcggct gtgaccgtcc
      661 cggaaataat ttcgtttttg tgaaggtttt ttttgatggt caacaatatc tgattgggat
      721 tgaccggctt gataaggtaa tcggagatct tgctgcctat ggcttggtcc atgatatcct
      781 cctcctcgct cttggtaatc ataactatag gcaaggaagg ataaagttcc ttgatggcgt
      841 tgagggtttc aaggccactg agcccgggca tattttcatc gagaaaaacc aggtcaaaaa
      901 cctggctgcg gcacaaatcc agggcgtcct ggccattggt ggccgtaacc agctcatagc
      961 ctttttcttc gaggaaaaga atatgaggtt tgagtaaatc aatttcgtcg tcggcccaaa
     1021 gcagcctttc ttttctcatt aaagcaatgc ctcaactttc tgtgccaatg cacttttttg
     1081 gatggctccc acttgtttgt ttaccaattg tccttgttta aaaaacaaga tggtcggaat
     1141 gtttctgatg ccgaaacgaa cgcaaacatc gttgttctcg tccacgttca tcttaccgat
     1201 gtttacccgg tcggcgtatt catcagctaa ttcttcgatg atcgggccta ccattctgca
     1261 gggtccgcac cagggcgccc aaaaatcgac tactaaagct ttaccggagg aaagatattc
     1321 ctcaaaattc gagtctgtta tttccaatgc catatgattc gtattttaat ttccttatac
     1381 attataacgt cggagcatga ctattatttg tcacaaagtt ataaaaaaaa ccacgttata
     1441 tactgttaat tcatttttat ttcaatctca ccgctttctt ccagaaagtc cgttatttcc
     1501 ttgtcgacct ggatgcgata attcctcgaa aacaaagaag catggagttt gggctggttg
     1561 tccggatcgt atacagtgat gtacaacagg gacttacccg gttttttatt gatcattgac
     1621 agaaaacgcc gggtaaattc attactaatc agatgcaaag gaatagtgat ctgaatttta
     1681 ttgacagcat tgcctgctgc ctctgaaagc aaagaaattg aattaatttt cagttcattt
     1741 tcggaagctt tccagggcct gggctggtag acccccctga aggccaaaaa catatctttt
     1801 ttgaggtatt tcccaaaatc tacatactcg cggctccaaa atggaagctc gacactggcc
     1861 gtataatctt ctattgttac aaaggcgtag ggatcgccgt tttgcttgcg tttttcgcgt
     1921 acttcggtca ctatgccggc aaattgtact tctttgttga gttccagttt gctcagcttg
     1981 atgtcttcta tggtggtgtt gcaaacgtat ttcataaaga ccgaatattc gtccagggga
     2041 tgcgacgaaa ggtacatccc gacgtattct ttttcacgat tgagacgttc cagttccgac
     2101 caactctccg ccctgggaat atccgggcgg gtaatggcca cctccatttc gcctccaaac
     2161 agggtgttct gattactggc cctgtccaac tgaattctgt tgccgtaacg cattaaaacg
     2221 tccagaaaaa cttcgccttt gtcgttttcg gcaaaaaatt gttccctttg tatttctccc
     2281 aggctgtcga aagctccggc caaagccatg acttcgatat ttttgcgggt acaggcattc
     2341 aggtttaccc gttcaaccaa atcgaagacg gaatcgtagg gcttgttggc acgttcttcg
     2401 actatggctt tgactgcgcc ctctcccatg cctttgatag ccccgagacc gaaacgaatg
     2461 ttgccttgtt tatttaccgt aaactgataa ttggactcgt tgatgtcggg tcccaacacc
     2521 aggattccca tggagcggca ttcgtccata aatttggtga tatcgcttat gttgtttaca
     2581 ctgcgactca acaccgcagc catgtattcc gaaggataat tagctttgag ccaggccgtt
     2641 tggtaggcca ccagagaata acaggtggca tgagatttat tgaaagcata ggaggcaaac
     2701 ttttcccaat cggcccagat cttgtcgagt atgccttcgt cgtagccgtt tttcttgcct
     2761 ccctcaataa atttaggcct gagtttgtcg agttgttccc ggatcttttt gcccatagcc
     2821 ttacgcagct cgtcggattc tccacgactg aaaccggcca gcaatctgga cagcaacatc
     2881 acctgttcct gatagacggt aatgccgtag gtgtctttca ggtaacgttc catcaccgga
     2941 aggtcgtact ctatggcttc actgccgtgc ttgcgggcaa tgaactgagg aatataatcc
     3001 atgggaccgg gccggtagag ggcattcata gcaatgaggt cttcgaactt ggaaggcttc
     3061 aaatcgcgca ggtatttttg cataccggcc gattcaaact ggaaagtacc cacggttttg
     3121 ccttcactgt agagttcata ggttttttcg tcctcgagcg atatgttgtc gatatctatc
     3181 gtgatgcccc gtgacaaacg gatattctcg acggcatcct taatgatgga tagggttttc
     3241 aaacccaaaa agtccatttt gatcaatccc gtatcttcga tgacagaacc gtcgaactgg
     3301 gtcaccagca atttttcgcc tgtttctttg tcttcggccg tgctcacggg cacccaatcg
     3361 gaaatgtcgt cgcggcttat aatcaccccg caggcgtgaa ctccggtatt acgaacggtg
     3421 ccttcgagct gctgggcgta tttcatggtg tcgcgcaagg cgggatcgtc cgattcggcg
     3481 gcctccttaa gttccggcac ataagcgatg gcgtttttca gattgacctt taggcgcctc
     3541 tgggaatcgg ccggatccgt aattttatcg ggaaccagct tggataaacg atccgattcg
     3601 gagagcggca atttttgtac cctggctaca tctttgatgg ccgatttgga ggccatggtg
     3661 ccgtaggtga tgatatgagc caccttctct ttgccgtaac gctcttctac ccagcgtaaa
     3721 acagcgtttc ttccgtcgtc gtcaaagtcg atgtcgatat cgggcagaga aatccgttcg
     3781 ggattgagaa aacgctcaaa cagcaaatcg tatttgatgg gatccacctt ggtaatgccc
     3841 aggcagtagg ctacagccga accggcggcc gaaccccggc ccggccccac cgaaacgccc
     3901 atattccggg cggctgtggt aaaatcctgt acgataagaa aatagcccgg ataacccatg
     3961 ttcttgatta cctccagttc aaattcgagt cgttcacgaa cttcttcgtt catatcggga
     4021 tagaccttct cggctccctt gtaggtgagg aaacgcaggt attcgttatc gtcgtcgaag
     4081 ccctcgggtt tggggaagtc gggcataatg ggttcgtggt tgatatcgta atattccacc
     4141 ttttcggcta cttccagcgt gttctcaagc acctggggca aatcatgaaa aacagcctgc
     4201 atttcttcga cggttttgaa ccattcctgt ttagtatagg ccattttgct gtaatcgtcc
     4261 acatcgttgc ccgtattcag gcaaataagc cggtgatgtg cttcggcatg gtcttcgtcg
     4321 acaaaatgca cgtcgttgct ggcaataagt tttacctggt atttttgagc cagttcaatc
     4381 aaatgcttgt ttaccagttg ttgcttggga taggtatcct gagccgcatt ggctaccgtg
     4441 ggtttatggc gttgcagttc cagataaaaa tcatcgccga acactttttt aaaccaacgt
     4501 accgattgct cagcctgttc caagttgtcg ttgagtatat agctgggaat ttcaccgccc
     4561 aggcaggctg aacaggctat caaaccttcc cggtaggttt ccagatcgcg gtgatcggtt
     4621 ctggcacaat aataataacc gtcggtccag gcgcgagaaa ctatttttat gaggttttta
     4681 tagcctttaa agttcttggc cagtaaaatc agatgccagc cccgagcgtc ttctttgacc
     4741 tctttgcgat gcatttcttt tccgttgcgg gcaacgtaca cctcacaacc gataatgggt
     4801 tttatgggag ttttcgactg ggcattcttt gctttaacat aattatgaaa ctctttcact
     4861 ccaaacatgc aaccgtgatc cgtcaaggct atggccggca ttttatttgc aacggctttg
     4921 tcgaccaagt cttgtataga ggcttgtcca tcgaggatgg aatactgaga atgcacgtgt
     4981 agatgtacga atgaactcat aatcaattgt ttgttaaaat gttacttcca gccttaaaga
     5041 taaagccgat ttgtcatatt cgggtcaagt atgtattaaa cttcgtccaa atctgtgtta
     5101 aaaaaaaagc ctgcgaattt ttcgcaggct tttttcgctt taaatgaatt agttaatgcc
     5161 taaaaaagga tggtgtatcc cagcgacaca aggggagtat aagctgcgcc ccaaccggtg
     5221 taattcaact ccaggccggc agccatggtg tcgttaaaga agtaacggcc tcccagcaat
     5281 ccgccatagg caaaaccatt gcctctgttt ttgttgtgtc cgaagcccag ttgaataccg
     5341 gcatgcactt caatagcgtc ggtaagaaaa ataccgtaag ttgcttttgg caaaataccg
     5401 aaattaaggc cttccgagga aatggccacc gtctgtattc ccagacctac ggcaaaatgg
     5461 ccttcccacc aagaatcgac aagtacgtat tcaccgctca agctggcacc caatccgggt
     5521 tggaaaccaa gagcagcagt agcggcaatg gttccttttt ccagaggtga acccagggca
     5581 ttggcattaa aattcacaaa catggccagt aaggaggcca gcaaaacaga tgtaagcgtc
     5641 tttttcataa taaaaatttt aataaatgag acatgtcagc aattatatcg ctttgtttta
     5701 tagcttctta attaattatc tttacaactc tttatcgggc gagatgtatt tctatcctca
     5761 aacttcagga agcaaactta catatttttt tgtaacatca atcttatttt ccgtaatttt
     5821 tcggaagatt cttcggaaag ctccataaac agatgcattt ccataataaa aatattgttg
     5881 tacttttgcg gttttaaggt ttaatgatga aaattccttt caggatatac attcataaag
     5941 agggatggaa gctcttgtct tcggttgctc ttggattgat tacgatcaac cttttgcttt
     6001 tttttctggc cggtggaatt acttttagta tcagcctggt tatttcttct gttttatttc
     6061 ttttttttac taattttttc agaagtcccc gccgttttta tcaacaaagt catccgggcg
     6121 acatagtgtc gccggccgat ggaaaagtag ttgtcgtcga acctgtttat gaaaatctgc
     6181 tcttgaaaga aaaacgcctg caagtgtcta tctttatgtc tgtcaccgac gtacacgcca
     6241 actggtatcc tttggccggc aaagtgattc attacagcca tcgaaacggc aattaccacg
     6301 cggcctggtt gcccaaagcc agcagcgaaa acgaacgttc cagcgttatt atccgtgccg
     6361 cctacaatca gcaaaccgtt ctggtacgcc aaattgccgg ggcactggca agacgcattg
     6421 tcacttacgc ccatacaggt aaacgctgta agctgaacga acatttgggc tttattaaat
     6481 tcggatccag agtcgatttg tttttccctc tcgactccgt tgatatgttt gttgaacaag
     6541 gagcacgtgt taagggcaac aaaacggtta ttgcccgctt taaagacaaa gaacgaaatg
     6601 agactgttta agtatcttcc caacgccata accttactaa acgccctggc aggctgctta
     6661 tcgattgtgt tcgcattcga aaaagcctac acggcagcag cggctctcat agcactggct
     6721 gccgtttttg attttctgga cggcttggcc gcacgcttgt tccaggccta ttctccgctg
     6781 ggcaaagagc tcgattctct ggccgatgtg atcagtttcg gactggcccc ggcaatgatg
     6841 gtattcaact attaccgaga tctgactccg ggcttgttga gtgaaaacac catacatata
     6901 ttggcttact cggcttttat ccttcccctg ttttcggctc tgaggctggc taaatttaat
     6961 atagacgaaa ggcaaagccg gggttttata ggcctcccta ctccggccaa tgcccttttc
     7021 tgggcttttg gtgttgccgc catacaggaa tacaatttgc cggtactatc tttagcaatt
     7081 cctatactta tcttgctgtt ttcctggttg ttggtatcgc ctatacccat gttttcgctg
     7141 aagttttcgc gctttaccat aagcggcaat cgcttgaggt atttctttct gtccggctgc
     7201 atagtgcttg tattgcttat tggttggcag gccattgcct tgtgtgtcgt ttggtatatt
     7261 ttgctttctc ttttcacaaa agcagataag catttgcaat agacaaataa aaagccttat
     7321 ttagcgtctg aaatccaaaa ccttgtctgc cttcgctcat gaaaaaaact aaggatccct
     7381 ccaaaaagag aaagaaaaaa ggtggccgga aaaaagtcct gccaaaatca aaaccccggc
     7441 tcagaaacaa acggattgta tttgcgctta acgagcctga atacaacgcc ctgctttcgt
     7501 attgcgaaaa gtacaacata agcaatcgct cgcatttaat tcgaaccgtt ttgatgacaa
     7561 ccatcctaaa aaggttcaac aaggattatc ccaccctgtt tcacgaggaa gaaatgcatt
     7621 gaatgagtct tcaccaaaaa tacatgcgcc tggccctgaa cgaagccttg ctggcttacg
     7681 aacaaaacga agttcccgtg ggagccgtcg tggtatgcaa gggcagagta attgcgcggg
     7741 ggcataacct gaccgaaact ctgaacgatg taacggctca cgccgaaatg caagccatta
     7801 aggcggcagc ctcaaccctg ggcgg
//
LOCUS       DAOSGL010000071         7763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8114_length_7763_cov_4.430202, whole genome shotgun sequence.
ACCESSION   DAOSGL010000071 DAOSGL010000000
VERSION     DAOSGL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8114_length_7763_cov_4.430202"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="PKY68_03560"
     CDS             <1..1254
                     /locus_tag="PKY68_03560"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOH24146.1"
                     /translation="PHITDEIKRSIRQLTHNGQYDFIITEIGGTVGDIESLPFIESVR
                     QLTWELGSSNCMCIHLAYVPYLSATKELKTKPVQHSVKLLLELGVQPDILVLRTEHPL
                     TTEIRKKVALFCNVNEKAVIECIDLPSIYEIPLKVRQENLDTIILEKFNLPVNTESDL
                     QPWIDFVNKMKNASEKVHIGLVGKYTSLPDAYISITESLKHASAYSDHCLDLRLISAE
                     KIEENNVEKTLSGLDGLLIGPGYGIRGIEGKISALKFAREQNLPTFGIGLGMQCMCIE
                     FARNVLGLKDATSTEFNPNTADNVIDLMDEQKSVANMVGTMRLGNFDCEVDKNSIAYR
                     AYQNEHIQERHRHRFEFNNNYKERFETAGMKCVGINPDTQLVEIVELSDKKWYLGTQF
                     HPEYNSTVLNPNPLFVDFIKACIDK"
     gene            1259..3133
                     /gene="yidC"
                     /locus_tag="PKY68_03565"
     CDS             1259..3133
                     /gene="yidC"
                     /locus_tag="PKY68_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOH24147.1"
                     /translation="MDKNTLIGFGLILLILVGFSMLQKPTEQKVQQAIQQDSIIQATP
                     QQPLSQQTAPYEQELVEAGQTEAQALNDNYGQFAAFLTGENQIYNIENDLMVVSMASK
                     GGRIASVRIKNYVTHDSLPLILFDEKNSSFDITLISSNNRIINTKDLYFTPVQGQNPL
                     EFIFRLALAQDQYLDFVYTLQEDSYLMSMDICGQGLENMLSPSLSNLDLNWAVKMRSQ
                     EKGRKFEQRYSMLQYKFMGDDVEKMSVNKDENKIVNNRLHWIAFKDQFFASVLICDKG
                     LISNELSSKLESDLSPHIKDYKLRSGIDFDMRGNREANLNFYFGPTKYSILKKFDKGL
                     KKSERLGLDNLVPLGGSIIRWVNVGLIIPMFNFFGSFIGNFGIIIILMTICIKLLILP
                     LTFKSYKSSAKMRLLKPQIDEINTKFAGESKAQERSAATMELYKRAGVNPMGGCLPML
                     LQFPILIAMFWFFPASIELRQESFLWAKDLSTYDAVISWEANIPFISKFFGNHISLFC
                     LLMTVTNLIYTKINSDNTGGSNQMPGMKAMMYMMPIMFLFIFNQYSSGLSFYYFVSTL
                     FTIGQTYIFRATIDEEKMLKQLMENKNKPIKKSSFAKRLEEAQRMQREQIRQQAKKRR
                     "
     gene            3176..4471
                     /locus_tag="PKY68_03570"
     CDS             3176..4471
                     /locus_tag="PKY68_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOH24148.1"
                     /translation="MKTKYHSDSPIRIAVVGLGYVGLPLARLFSTKYPTRGFDIDPLR
                     VEAIMQGRDTTGEVSPEVLAEALGKGFSCSSKLSDIKDCNYYIVCVPTPIDKNNHPIL
                     EPLVQASGLLAKVLKKGDIVIYESTVYPGVTEDICVPVLEKNSGLQFNRDFFVGYSPE
                     RVNPGDRVHSIEKIIKVTSGSTPATADKVDALYNSVLLNGTYKVSSIRVAEASKIVEN
                     AQRDVNIAFINELARIFNAMNLDTKEVLDAAATKWNFIRLSPGLVGGHCISVDPYYLI
                     EKAHIHGYLPRMMSDARQLNNSMGSYVGHRVLKLMNKKGLLVKDARILILGVTFKENC
                     PDTRNTKVVDVYNTLREYTSNIVLYDPHADPAGFQKEFGLPLLREGLENRKEKFDVVV
                     LAVAHEAFRKLDIRGLLNKNGLVYDVKSFLPRELVDDRL"
     gene            complement(4492..6138)
                     /locus_tag="PKY68_03575"
     CDS             complement(4492..6138)
                     /locus_tag="PKY68_03575"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOH24149.1"
                     /translation="MTKSALQIARAEYKPKMPLALLGPVKIKEGAPTESVSDKEEIQK
                     LFPNTYGQPIIEFEPSSQHSSFPPINVGVILSGGQAPGGHNVIAGIFDGIKSLNAGSR
                     LFGFILGPAGLIEHNYMELTADIIDEYRNTGGFDMIGSGRTKLETKEQFDRGLLILKA
                     LDIKALVIIGGDDSNTNACVLAEYYKSINAGVQVIGCPKTIDGDLKNEMIECSFGFDT
                     ACRVYSEVIGNIQRDCNSSRKYWHFIKLMGRSASHIALECALQVQPNVCLISEEIEAK
                     NQSLDDVVTEIAKIVARRAEQGNNFGVVLIPEGVIEFIPAMKALIAELNEFLMEYQSE
                     FGLIRKSEQRNYVINKLSPKNSAVFASLPENVAMQLTLDRDPHGNVQVSMIETEKLLA
                     EMITTKLAKWKAEGKFVGKFATQTHFFGYEGRCAAPSNYDADYCYSLGYTASRLIAYG
                     KTGYMSSVRNTTKPAKDWIAGGVPITMMMNMERRHGEMKPVIQKALVDLKGKPFRTFV
                     SKRAAWAIQTDYVYPGPIQYFGPTEVCDQPSKTLQLESGS"
     gene            complement(6240..7424)
                     /locus_tag="PKY68_03580"
     CDS             complement(6240..7424)
                     /locus_tag="PKY68_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOH24150.1"
                     /translation="MSEKFKINRFVHYVGVNDRKTHLFENLWPLPLGVSYNAYLVVGE
                     KIALIDTVESCYASELLEMIVRTVGNRPLDYLIVNHMEPDHSSSINAIRQAYPGIQIL
                     GNAKTIQMLEGYYGAHQGIIEVKEGDSLCLGGETLRFYLTPMVHWPETMMTYCEQEQL
                     LFSGDAFGTFGALNGAVLDQDMDLSPYWDEMYRYYANIVGKYGSPVQKALQKLSGLDI
                     RVICPTHGPVWKKELNKVVGIYDKLSRYEGEKGVVIAYGSMYGNTARTAMRMSEELAS
                     LGLETVVMHNLSVSSLSDVLRDVFKYRGLVIGSPTYNAGLFPPVEALVNALAARNVKN
                     KVYACFGSFTWAGAAAKKLQEFGVTMNWEIAGEAVELKQGYSREKTEACALLAKNLLA
                     KL"
     gene            7563..>7763
                     /locus_tag="PKY68_03585"
     CDS             7563..>7763
                     /locus_tag="PKY68_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24151.1"
                     /translation="MQTIRFCSRLRFCALCLLFSLAGTLQAQEWKLVWSDEFNTEGPP
                     DSGSWNYERGFVRNEELQWYQPG"
BASE COUNT         2213 a         1880 c         1699 g         1971 t
ORIGIN      
        1 cctcatataa ccgacgaaat aaaacgctcc attaggcaac tcacccacaa tgggcaatac
       61 gatttcatca tcaccgaaat tggcggaacc gtaggcgaca ttgaatcttt gccgtttatt
      121 gaaagcgtgc gtcagttaac ctgggaattg ggtagcagca attgcatgtg catccacctg
      181 gcttatgttc cctatctgtc ggcaaccaag gaactaaaaa ccaaaccagt acaacattcc
      241 gtcaaattac tgctcgaatt gggggtacaa cccgacattc ttgtactgcg caccgaacac
      301 cccctgacta cagagatacg caaaaaggta gccttattct gcaacgtaaa cgaaaaagcc
      361 gttatcgaat gcatcgattt gcccagtatt tatgaaattc cgcttaaagt gcgccaggaa
      421 aacctggaca ctattattct ggagaaattc aatctgccgg taaatacaga atccgacctt
      481 cagccatgga tcgatttcgt caacaagatg aaaaatgcca gcgaaaaggt tcacataggt
      541 ctggtgggca aatataccag tctgcccgac gcttatattt ccattacgga atcgctgaaa
      601 cacgcctcgg cttacagcga ccattgtctg gacctgcgct tgatatcggc cgaaaaaata
      661 gaagaaaaca atgtggaaaa aacgctctcg gggctggatg gtctattgat cggccccggt
      721 tacggaataa ggggtatcga aggtaaaatt tctgccttaa aatttgcacg ggaacaaaac
      781 ctgcccactt tcggcattgg cctgggtatg caatgcatgt gcatagaatt cgcccgtaac
      841 gtattgggcc tgaaggatgc cacctccacc gaattcaatc ccaatactgc cgataatgtc
      901 atcgacctga tggatgaaca aaaaagcgtc gccaatatgg taggtaccat gcgcttgggt
      961 aatttcgatt gcgaagtaga caagaattcc atcgcctatc gcgcctacca aaacgagcat
     1021 attcaggaaa gacatcgcca ccgctttgaa ttcaacaaca actacaagga aagatttgaa
     1081 actgccggca tgaaatgcgt gggcatcaat cccgacaccc aattggtgga aatagtggaa
     1141 ctcagcgaca aaaaatggta tctcggcact caattccatc cggaatacaa tagtaccgta
     1201 cttaacccca accctttgtt tgtagacttt atcaaagcct gcatcgacaa ataatattat
     1261 ggataaaaat actttaatcg gatttggact tattttattg attcttgtcg gattcagcat
     1321 gctccaaaaa cccacggagc aaaaagtgca acaagccatc caacaagaca gcataataca
     1381 agcaacacct caacaaccgc tcagccagca aacggcccct tacgaacaag aactggtaga
     1441 agccggacaa accgaagctc aggcactcaa cgacaactat ggtcagtttg ctgcttttct
     1501 taccggcgaa aaccaaattt acaacatcga aaacgacctg atggtggtca gcatggcttc
     1561 caaaggagga cgcatcgcct ccgtccggat caaaaactac gtcactcacg attctctacc
     1621 cttgattctg tttgacgaaa agaattcttc gtttgatatt acccttataa gctccaacaa
     1681 tcgtattatc aacaccaaag acctctattt cactcctgtg cagggacaaa accctctgga
     1741 atttattttc aggttggcat tggctcagga tcagtatctt gactttgtgt ataccttgca
     1801 agaagacagc tatttgatgt ctatggatat ctgcggacag ggactcgaaa atatgctctc
     1861 gcccagtctg agcaatctgg acctgaattg ggccgtcaaa atgcgcagtc aggaaaaagg
     1921 ccggaaattt gaacaacgtt attccatgct gcagtataaa tttatgggag acgatgtcga
     1981 aaaaatgagt gtaaacaaag acgaaaacaa aattgtgaac aaccgcctgc actggatagc
     2041 tttcaaggat cagtttttcg cctccgtact gatttgcgac aaaggtctta tttccaatga
     2101 attaagctct aaattagaaa gcgatttgtc gccccatatc aaagattaca aactacgttc
     2161 gggcattgat tttgatatgc gaggcaacag ggaagccaac ttgaattttt atttcggccc
     2221 caccaaatat tccattctca agaaatttga caaaggcctc aaaaaatcgg aacgactcgg
     2281 tctggacaac ctggttcccc tgggaggcag cattatccgt tgggtcaatg tgggcctgat
     2341 cattcccatg ttcaattttt tcgggagctt tatcggtaat ttcggtatca tcatcatttt
     2401 gatgaccatc tgtatcaaac tgctgatcct gcccctgacc tttaagtcgt ataagtcgtc
     2461 ggccaaaatg cgtttgctca agccccagat tgatgagatc aacaccaaat ttgcaggcga
     2521 aagcaaagct caggaacgtt ccgccgccac catggagctc tacaaacgcg ccggagtcaa
     2581 tcctatggga ggctgtctgc ctatgttgct gcaattcccc atactgattg ccatgttctg
     2641 gttttttcct gcttccattg agctaaggca ggaaagtttc ctctgggcca aggacttgtc
     2701 cacttacgac gccgtcatta gctgggaggc caatattccg ttcatcagca aattctttgg
     2761 taaccacatc agcttgtttt gtttgctgat gaccgtcacc aacctgattt acaccaaaat
     2821 aaacagcgac aacaccggcg gaagcaatca aatgcccggt atgaaggcca tgatgtacat
     2881 gatgcccata atgttcctgt ttattttcaa tcaatattcc tccgggttga gcttctacta
     2941 cttcgtttca actttgttta ccattggaca aacctatatt ttcagagcaa cgattgacga
     3001 agaaaagatg cttaaacaac tgatggaaaa caagaataaa cccatcaaaa aatcgagctt
     3061 tgccaagaga ctcgaagagg ctcaacgcat gcaaagagag caaatccgac aacaagccaa
     3121 aaaaaggcgt taaaaataac ggatgcccgg gcatccattc aagaaatttt cggccatgaa
     3181 aacaaaatac cattcagact cccctatccg cattgcggtc gttggactgg gttatgtggg
     3241 tttgcctctg gccaggcttt tctctaccaa atatccgacc aggggattcg acatcgatcc
     3301 gctcagggtg gaagccatca tgcaaggcag ggacactacc ggcgaagtaa gccccgaagt
     3361 cctggccgaa gcccttggaa agggatttag ctgcagttcc aaactgtctg atataaaaga
     3421 ctgcaattat tacattgtat gcgttcccac gcccatagat aagaacaacc accctatact
     3481 ggaaccgctc gttcaggcca gcggcttgct ggccaaagtg ctgaaaaaag gggatattgt
     3541 tatttacgaa tctaccgttt atccgggcgt caccgaagat atttgcgtac cggtactgga
     3601 aaaaaattcc ggcttgcagt ttaacaggga tttttttgta ggttattctc ccgagcgtgt
     3661 caaccccggc gatcgtgtcc acagcataga aaaaatcatc aaagtcacct cgggctcaac
     3721 tcccgcaaca gccgacaaag tggatgcttt gtacaattcc gttttactga acggcaccta
     3781 caaagtgtcg tcgatacgag tggccgaagc ctccaaaatc gtcgaaaatg cacagcgcga
     3841 cgtcaatatt gctttcatta acgagttagc taggatattc aacgctatga atctcgatac
     3901 caaagaagta ctggacgccg ctgccaccaa atggaatttc atacgcctca gccccggact
     3961 ggtaggtggt cattgcataa gcgtcgaccc ctactatctg attgaaaagg cgcatattca
     4021 cggttacctg ccccgcatga tgagcgatgc ccgtcaactt aacaacagca tgggcagcta
     4081 cgtaggccac cgggtgctca aactgatgaa caaaaaaggc ctgttggtga aagacgccag
     4141 gatactgatt ctgggagtga ctttcaagga gaactgtccc gatacacgca acactaaagt
     4201 ggtggatgtg tacaataccc tccgcgaata tacctccaat atagtgcttt acgaccccca
     4261 cgccgacccg gccggtttcc aaaaagaatt cggcctcccc ttactcagag aaggcctgga
     4321 aaatcgtaaa gaaaaattcg atgttgtggt gttggcagtg gctcacgaag cattcagaaa
     4381 gctggatatc agaggcctgc tcaacaaaaa cggattggtc tacgacgtca agagcttttt
     4441 gccacgggaa ctggtcgacg acaggcttta atcaacactt tcttgattca attaactgcc
     4501 ggactccagt tgcagagtct tgctcggttg atcgcagacc tcggtcggac caaaatactg
     4561 gatgggaccg ggataaacat aatcggtttg aatggcccag gcggctcgtt ttgaaacgaa
     4621 agttctgaat ggtttaccct taagatctac caaggctttt tgaattacgg gtttcatttc
     4681 accgtgacga cgttccatat tcatcatcat ggtgatgggt actccgccgg caatccagtc
     4741 tttggccggt ttggtggtgt tgcgtacgga agacatgtaa ccggttttgc cgtaggcaat
     4801 cagtcgtgaa gcggtataac ccaacgaata gcaatagtcg gcatcgtagt tggaaggagc
     4861 ggcacaacgt ccttcgtaac cgaagaagtg agtttgagtg gcaaacttgc ccacaaactt
     4921 accttcggct ttccacttag ccagtttggt tgttatcatc tcggccagga gcttttcggt
     4981 ttcgatcata gaaacctgca cgttgccgtg aggatcgcgg tccagcgtaa gctgcatagc
     5041 cacgttttcg ggtaatgagg caaatacggc cgagtttttg gggctcagtt tgttgataac
     5101 gtaattacgt tgttcggatt tacgaatcag tccgaactcg ctttggtatt ccatcaggaa
     5161 ttcatttaac tccgcaatca aagccttcat ggcgggaata aattcaatta cgccttcggg
     5221 aattagaaca acaccaaaat tatttccttg ttcggcccgg cgggctacta tcttggctat
     5281 ctccgttacc acatcgtcga gcgattggtt cttggcctcg atttcctccg aaataagaca
     5341 gacattgggc tgcacctgta aagcgcattc gagcgcaata tgcgaagccg aacgtcccat
     5401 gagcttgata aaatgccaat atttgcgcga tgaattacag tcgcgctgta tattgccaat
     5461 aacttcggaa tatactctgc aggcggtatc aaaaccaaaa gagcattcaa tcatctcgtt
     5521 cttaagatcg ccgtcgatgg ttttggggca acctattacc tgcactcctg cattgatgga
     5581 tttataatat tcggccagca cgcaggcatt ggtattggaa tcgtctcccc caataataac
     5641 caaagctttt atatcgagtg ccttgagtat aagcaaaccc ctgtcgaatt gttctttggt
     5701 ttcgagcttg gtgcggcccg agcctatcat gtcgaaaccg cctgtattgc gatattcatc
     5761 gatgatgtca gccgtcagtt ccatataatt gtgctcaatc aaaccggcag ggcccaaaat
     5821 aaagccaaac aaacgggatc cggcgttcaa agatttaatg ccgtcgaata tgccggcaat
     5881 gacgttgtgt ccaccgggag cctgaccgcc cgataaaata actcctacgt ttatgggagg
     5941 aaagctactg tgttgactgg aaggttcaaa ctcaataatg ggctgcccgt aggtgttagg
     6001 aaaaagtttt tgaatctcct ctttgtccga aaccgattct gtgggagcac cttcttttat
     6061 ttttaccggc ccaagcaatg ccaggggcat tttgggctta tactctgcac gagcaatttg
     6121 taaagcactt ttggtcataa agggaataaa taaagttcgt aaaaaaacga cacaaatttc
     6181 gtgttttttt tgttcaccca caagccggcg agggctaaat agtaattttt ataaaacact
     6241 taaagtttgg ccaacaagtt tttggctaac agggcacaag cctctgtttt ttcgcgactg
     6301 taaccttgtt tcaattctac agcctctccg gcgatttccc agttcatcgt gacaccaaat
     6361 tcctgcagct ttttggcagc cgcacctgcc catgtaaaag agccgaaaca ggcatagact
     6421 ttgtttttga cattacgggc tgccagagca ttcactaaag cttctacagg aggaaacagc
     6481 ccggcgttgt aggtaggaga acctatcacc agaccacggt acttgaacac atcacgcaac
     6541 acatccgaaa gagagcttac cgacagattg tgcataacaa cagtctctaa acccagactt
     6601 gccaactcct cagacatgcg catggctgtt cgggccgtat tgccatacat gcttccatag
     6661 gctattacca cccctttttc tccctcgtat cgactgagtt tatcgtaaat gccgacgacc
     6721 ttgttgagtt cttttttcca gacgggacca tgggtagggc aaattacccg aatatccagg
     6781 cccgaaagtt tttgcaaggc tttctgtacc ggcgatccgt acttaccgac aatattggca
     6841 taataacggt acatttcgtc ccaataagga ctcaggtcca tatcctgatc caacacggca
     6901 ccattcaggg ccccgaaagt gccgaatgca tcgccggaaa aaagcaactg ttcctgctcg
     6961 caataggtca tcatggtttc gggccaatgt accatagggg tcaggtaaaa acgcagggtt
     7021 tcgccgccca ggcaaaggct atcaccctct tttacctcga tgattccctg gtgtgctccg
     7081 taatagcctt cgagcatttg tatggtcttg gcattaccca ggatctgaat gccgggataa
     7141 gcctgacgta tggcatttat cgaagaagaa tgatcgggct ccatatgatt gacgatcaga
     7201 taatccaaag gacgattacc caccgtccgg actatcattt cgagcaattc cgaagcataa
     7261 caggattcaa ccgtatcgat aagagctatt ttttctccaa caaccaaata ggcgttgtac
     7321 gaaacaccca aaggcaaagg ccagagattt tcaaaaagat gagttttgcg gtcgttcacg
     7381 cctacgtagt gaacaaagcg gtttatttta aatttttcgg acattgaatt attatttttt
     7441 gcaaagttaa tttttccgct ctaaacagac aagagcaaga actcctttca ataatcatat
     7501 ccatagtaca ttttttaaaa agctacgaat atagcctatt tttgtcccat aaaaaacgac
     7561 acatgcaaac gatcagattt tgttcaagat tgagattttg tgccttgtgc ctgctgttta
     7621 gccttgccgg aacacttcag gcgcaggaat ggaaattagt ctggagcgac gaattcaaca
     7681 cagaagggcc acccgattcc ggcagttgga attacgaacg cggatttgta cgcaacgagg
     7741 aattgcagtg gtaccagccc ggc
//
LOCUS       DAOSGL010000072         7700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8191_length_7700_cov_3.934075, whole genome shotgun sequence.
ACCESSION   DAOSGL010000072 DAOSGL010000000
VERSION     DAOSGL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8191_length_7700_cov_3.934075"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2719
                     /locus_tag="PKY68_03590"
     CDS             <1..2719
                     /locus_tag="PKY68_03590"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HOH24152.1"
                     /translation="SGYGPGGTEIIANYRDRTTLIRFKGVDNRSTGTCYTIADESLPV
                     GTMQMVLPGHRLKAGDHIRISLPARSSQQAASGQNASGQEAAPARMRNTPSMQWEREI
                     TSVDGDKVCLDAPLTYEISTRNGSADVCLLGWPGRIRQVGLENLRLTSVYDQSNPKDE
                     NHAWIAVAMENVRDAWVRRVEFRHFAGSAVYLTETASRITVEDCKSLQPVSEIGAERR
                     YSFYTLGGQCLFQRLWAEYGYHDFAVGAGAPGPNAFVQCWSISPHSFSGTVDALSSGV
                     LFDICAVHANALRFSRPDPANEGYSYTTANSMFWNSTAAIMSCPKPGSAQNWAFGAWA
                     QFSGKGYWHEANSHVSPWSLFYAQLRDRTGKRNPGENKLITLSQGGTSSREDARRETL
                     AARKPLILLSDWIDTLCLREPLSLTYDNKDKQLSKVWQQEPYMIKKAEKSPAPALQLS
                     EGVIIRDGKILTGGRFNPAWWRGSLLPKNQQAPHLTRYALEPEGYRVVDDLNEVCGNM
                     QKSGILVMDHNYGLWYDRRRDDHERTSRIDGEVKAPFYELPFARSGQGRAWDGLSKYD
                     LTQWNVWYWNRLKKYADLADDKGLVLFHQHYFQHNIIEAGAHWADFPWRSANNVNETG
                     FPEPVPYAGNKRVFMAEHFYDVTHPVRRELHRNYIRKCLDNFAENSSVLHFISAEFTG
                     PLHFVEFWLDVVSEWEKETGCNALIALSTTKEVQDAILKDPVRSKVVEAVDIRYWYVD
                     ENGKEFAPKGGLNLAPRQFERMEKPAKTSASEVYNMVSSYRRQYPQKAVVYSADSYPE
                     FAWAAFMAGASFCVLPKDLPEDFLKDAASLSPLAAACWLMAKAGQAYIAYPSDDALIE
                     VDLTKETKPFRAQWIDPVSGRTKGKVFQVRAGTVFKTTGKDVLWLRQTN"
     gene            2781..3725
                     /locus_tag="PKY68_03595"
     CDS             2781..3725
                     /locus_tag="PKY68_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOH24153.1"
                     /translation="MKKFLVPFLTVLLFTACTPKEVYMFSSFNEPATDGLRLLYSEDA
                     YHWEDLGGIFLKPAVGNQQVMRDPSIVRAPDGTFHLVWTSSWRGDLGFGYASSKDLIN
                     WSEQRFLPVMAFDTTTVNVWAPEIHYDETQAQFIIIWASCIPYRFEKGIEEETGNYRM
                     YYVTTKDFQEFSETKLFYDPGFSVIDAVMVPRAKDDVVLVLKDNTRPERNLKVAFGSS
                     MTGPFGPASEPFTEIYTEGPSVVKVADGWLIYFDAYRKKTYEAVFTKDFVHFENANDQ
                     ISIPEGHKHGTIFKADHKVLKALKKEMEKRQQVESNNQ"
     gene            3737..5617
                     /locus_tag="PKY68_03600"
     CDS             3737..5617
                     /locus_tag="PKY68_03600"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOH24154.1"
                     /translation="MKYSAISTLLLLFVLTVSCSQTASKSGPLADRVHYTGTSMSNVD
                     YHHGQLSPAIGVHNVQIMRANRGKPESSDGYGWTYNHAPMMAYWSNTFFVEYLSDSVS
                     EHIPPCHTLLVRSSDGINWSKPEVIFPKYKIPDGFKKPGSEVVAKDAIAVMHQRVGFY
                     VSSHNKLLALAYYGISLFPKDDPNDGMGVGRVVREIKADGTYGPIYFLRYNQSFNEEN
                     SLYPFYKSCPEADFVAACDEILANPLYMMQMVEEADRNDPLIPLQKQYKAFSYYHLPD
                     GRVVGLWKHALTSISADGGKTWAEPVERARGFVNSNAKIWGQRTSDGKYATVYNPSEY
                     RWPLAVSTSKDGLEYTDLMLVHGEITAMRYGGQYKSYGPQYVRGIQEGNGTPPDGKMW
                     LTYSMNKEDIWVSSVPVPICSKAAKHTREVFDEMPDGCETALWNTYDLQWASTRIEKD
                     EAGRKSLTLRDKDPFDYAKAEKIFPASAQFTVTAEIQTVQNDHGTLHMEVQDAKGQPS
                     IRISFNDDGRMYSKSGARYSSLGSYEAGKSYTIKLNVDTKMRYYTVQINSGREVGRIF
                     YAPAASLERVVFRTGEPRLHPTPETPADRYTDLPAADAVDPEACFRIYSLETAPATGN
                     NQ"
     gene            5632..7200
                     /locus_tag="PKY68_03605"
     CDS             5632..7200
                     /locus_tag="PKY68_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24155.1"
                     /translation="MKKQLVALLLIGFTVLSFASCQAKTKTPKSANILKAKDFKHYVD
                     YFNRMEDEPIVQAIPNAESWDWMQANIPLFSCPQDNFEEMWYFRWWSLRKGIRETPLG
                     FVINEFLVERSYADKYNMISCALGHHIYETRWLHDSKYLDQYLHVWFRGNEGAPMKRL
                     RSFSSWTAYAVYNRYLADGNKEYMLDLLPDLEKEYRDWEGDHRLPDGMYWQGDVQDGM
                     EESVSGGRRIKNARPTINSYMYGNALALSKMFSLSGETAKAALFAAKADTIKKMVQDN
                     LWHPERKFFETRWVNGQLAQAREAIGYIPWYFNLPDLGKNYEEAWLQVKDPEGFLAPY
                     GLTTAERRHPEFRTRGVGQCEWDGAVWPFASSQTMTAMANVLNNYEQNYVDKATYYEL
                     MNLYVESQYHRGRPYIGEYLDEVTGYWLKGDQLRSRYYNHSTFADLIITGLMGLRPGE
                     DNILVVNPLIPQEQWDWFCLDNLMYHGKNIAVVWDKSGKKFNKGQGLLIFCNGKKVAQ
                     KPTIEKITVDISPY"
     gene            7270..>7700
                     /locus_tag="PKY68_03610"
     CDS             7270..>7700
                     /locus_tag="PKY68_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH24156.1"
                     /translation="MAFKKKVSLKDIAQAVGVSSALVSMVLNGKAKQYGIGEEATRKV
                     LAKAKELNYQPNIMARGLRSGRSRLLGLVVADISNPFFATLAREIERAASERGYTVIY
                     GSSDEKPEHLSKLMNTLANQGVDGIIVVPCENSEELIADLYL"
BASE COUNT         2054 a         1812 c         1919 g         1915 t
ORIGIN      
        1 aagcggatac gggccggggg ggaccgaaat aatagcaaac tacagggaca ggacgactct
       61 gatccgtttc aaaggagtgg acaacagaag caccggaacc tgttatacca ttgccgatga
      121 atcgctgccc gtaggaacga tgcagatggt tttgcccgga catcgcctta aagccggaga
      181 tcatatccgt atcagtttgc ccgctcgcag ctctcaacag gcagcctccg gacaaaatgc
      241 ctccggacaa gaagcagccc ctgcccgtat gaggaatacg ccctcgatgc aatgggaaag
      301 agaaatcact tccgtcgacg gcgataaagt atgccttgat gctcccctga cctacgaaat
      361 ttcgacacgg aacggatcgg ccgatgtttg tttgttgggt tggccgggcc gaattcggca
      421 ggttggcctc gaaaatcttc gtttaacttc ggtttatgac caaagcaatc ccaaagacga
      481 aaatcacgcc tggatagctg tagctatgga aaacgtccgg gatgcctggg tacgaagagt
      541 ggaatttagg catttcgcgg gctcggcggt ctatcttacc gaaacggctt cccgcatcac
      601 cgtagaagat tgtaaatccc tgcagcctgt gtccgaaatc ggggcagaaa ggcgttacag
      661 cttttatacc ttgggtggac aatgtctgtt tcagcgcctg tgggccgaat atggatacca
      721 tgattttgcc gttggagccg gagctcccgg acccaatgct tttgtacaat gctggtcgat
      781 ctcgccgcat agttttagtg gtactgtcga tgctttgtcg agcggggttt tgtttgatat
      841 ttgcgctgtg catgctaatg ccttgcgctt ttcgaggccc gatccggcca acgaaggtta
      901 cagctatacc actgccaatt ccatgttttg gaattcaacg gcagccatta tgtcctgccc
      961 caaaccaggc tcagcacaaa attgggcttt tggggcctgg gctcagttct ccggcaaagg
     1021 ctactggcac gaggcgaaca gccatgtttc tccctggagt ttattttacg ctcaacttcg
     1081 cgacagaaca ggcaaaagaa atcccggcga aaataagttg attactctat cgcagggagg
     1141 aacaagctcc agagaagatg cccggcgtga aaccctggcc gcccgtaagc ctctgatatt
     1201 gctttccgac tggatcgaca ccttgtgtct gcgtgagccc ctcagtctca cttacgataa
     1261 caaagacaag caattgagca aagtctggca gcaggaaccc tacatgatca aaaaggcaga
     1321 aaaatctccg gctccggctt tgcaattgag cgagggcgtt ataatacggg atggaaagat
     1381 actgacggga gggcgcttta atcccgcctg gtggagaggc agtctgctgc ctaaaaacca
     1441 acaggctccc cacctgacgc gttacgcttt ggagcccgaa ggctaccggg tggtcgacga
     1501 tctgaacgaa gtgtgcggca atatgcaaaa aagcggcatt ttggtaatgg atcataacta
     1561 cggattatgg tacgaccgcc gcagggacga ccacgaacga accagccgga tagacggcga
     1621 agtcaaagcc cccttttatg aattaccttt cgcccgcagc ggacagggca gagcctggga
     1681 cggactgagc aaatacgatc tgacccaatg gaatgtatgg tattggaacc gtttgaaaaa
     1741 atatgcagat ttggccgacg ataaaggcct ggtattgttc catcagcatt atttccagca
     1801 caacatcatc gaagccggtg cccattgggc cgatttcccc tggcgttcgg ccaacaacgt
     1861 aaacgaaacg ggttttcccg agccggtgcc ttacgccgga aacaagaggg tgtttatggc
     1921 cgaacatttt tacgatgtaa ctcatccggt acgcagggaa ttgcaccgaa actacatccg
     1981 caaatgcctg gataactttg cagagaattc ttcggtgctg cattttatca gcgccgagtt
     2041 taccggtcct ttgcattttg tggaattttg gttggatgtt gtttccgagt gggaaaaaga
     2101 aaccggttgt aacgcgttga ttgccctaag taccaccaag gaagtgcagg acgccatttt
     2161 gaaagacccc gtgcgttcaa aagtggtaga agcggtcgat attcgctatt ggtatgtgga
     2221 tgaaaacggc aaagagtttg cccccaaagg tggattgaat cttgctccac gccaatttga
     2281 acgtatggaa aaaccggcca aaacctcggc ctcggaggtt tataatatgg taagcagcta
     2341 tcgccggcaa tatcctcaaa aggctgtggt ctattcggcc gattcttacc cggaatttgc
     2401 ctgggcagct tttatggccg gcgcctcttt ctgcgtatta cccaaagacc tgcccgaaga
     2461 ttttctgaag gacgcagctt cgctcagccc ccttgctgct gcctgttggc taatggctaa
     2521 ggccggacag gcttatattg cctatccgtc ggacgatgcc cttatagagg tagatctaac
     2581 taaggaaacc aagcccttcc gggcgcaatg gatagacccg gtcagtggcc ggacgaaggg
     2641 taaagtcttt caggtacggg ccggcaccgt atttaaaacc actggcaaag atgttttgtg
     2701 gttacgccaa acaaactaaa agaatgttta ttctataaag caaaatgtat tctattcaaa
     2761 taacccaaaa acaagctatt atgaaaaagt ttcttgttcc ttttttgacc gttctgttgt
     2821 ttacggcttg tactcccaaa gaggtttaca tgttcagctc attcaacgaa ccggctaccg
     2881 atggcctgag gctcttgtac agtgaagatg cttatcactg ggaagacctg ggcggtattt
     2941 ttttaaaacc cgcagtcggg aatcagcagg tgatgcgcga tccctctatt gtcagggctc
     3001 ccgacggaac ctttcatctg gtatggacct cttcctggag gggagatttg ggtttcggct
     3061 atgcttcgtc caaagatttg attaactggt cggaacaacg cttccttccg gttatggctt
     3121 ttgatactac caccgtgaac gtgtgggctc ccgaaattca ttacgacgaa actcaggctc
     3181 agttcatcat tatttgggct tcttgtattc cttaccgttt cgaaaagggg attgaggaag
     3241 aaaccggtaa ctaccgtatg tattacgtca ctaccaaaga ttttcaggag ttttccgaaa
     3301 ccaagttgtt ttacgatccc ggcttcagtg tgattgatgc tgtgatggtt cctagagcaa
     3361 aagacgatgt ggtgttggta ctcaaagaca atacccgtcc ggaacggaac ctgaaagtgg
     3421 ccttcggttc ttccatgacc ggcccctttg gcccggctag tgagcctttt accgagattt
     3481 ataccgaagg accatcggtg gtaaaagtgg cggatggttg gttgatctat tttgacgctt
     3541 atcgcaagaa aacctacgaa gctgtgttta caaaagactt tgtacatttc gaaaacgcga
     3601 acgatcaaat aagtattccc gaaggacaca aacacggcac tattttcaaa gcagaccata
     3661 aagtgctgaa ggctttgaaa aaagaaatgg aaaaacgcca acaagttgaa tcgaataacc
     3721 aataatgtcc tcaacaatga aatattctgc tataagcact ttgttgctct tgtttgtttt
     3781 gacggtatcc tgttcgcaaa ctgcttccaa atccggccct ttggcagaca gggtgcatta
     3841 taccggaaca agcatgtcca atgtagacta tcaccacgga caactgtctc cggccatagg
     3901 tgttcacaat gttcagataa tgcgtgccaa ccggggtaag cctgaaagtt cggacggtta
     3961 cggttggacg tacaaccacg ctcccatgat ggcctattgg agcaatactt tttttgtgga
     4021 atacctcagc gattccgtca gcgagcatat tcctccctgt catactttgt tagtccgctc
     4081 ttcggacggt ataaactggt caaaacccga agtgattttt cccaagtata aaattcccga
     4141 cggttttaaa aagccgggca gtgaggtggt ggccaaagac gccatagccg tgatgcatca
     4201 gcgggtgggt ttttatgttt cctcacacaa taaactgctg gcattggctt attacggaat
     4261 atctcttttc cccaaggacg atcccaacga cggtatggga gtaggccggg tagtacgcga
     4321 aataaaagcc gacggcacct acgggcccat ttattttttg cgttacaatc aatctttcaa
     4381 tgaagagaac agcctttacc ctttttataa aagctgtccc gaagccgatt ttgtggcggc
     4441 ttgcgatgaa atactggcca atcctctgta tatgatgcaa atggtggaag aagctgatcg
     4501 caacgatccg cttattccgc tccaaaaaca atacaaagcc ttctcgtatt atcatctgcc
     4561 cgacggccgg gtggtaggtt tatggaaaca tgcgcttact tccattagtg cagacggggg
     4621 aaaaacctgg gccgaaccgg tagaacgggc caggggtttt gtcaacagca atgccaagat
     4681 atggggacaa cgtacttcgg acggcaaata cgccaccgtt tacaatccct cggaatatcg
     4741 ttggcctctg gccgtttcca cctccaaaga cggactggaa tacaccgatc tgatgctggt
     4801 gcacggcgaa ataacggcca tgcgctacgg aggacagtat aaatcttacg gcccccagta
     4861 tgtacggggc attcaagagg gcaacggcac tcctcccgac ggaaaaatgt ggctgaccta
     4921 cagtatgaac aaagaagata tttgggtatc ctcggtacct gtaccgattt gtagtaaggc
     4981 cgctaaacat accagagagg tctttgacga aatgcccgac ggttgtgaaa cagctttgtg
     5041 gaatacctac gacctgcaat gggcaagcac ccgcattgaa aaagatgaag caggccgtaa
     5101 aagcctcact ctacgcgata aagatccttt tgattacgct aaagccgaaa agatatttcc
     5161 ggcttccgca caatttacgg tgaccgccga aatccaaacc gtacaaaacg atcacggaac
     5221 tttgcatatg gaggtacagg acgccaaggg gcagccctcc atccgcatca gcttcaacga
     5281 cgacggcaga atgtacagca aatccggagc tcgctattcc agtctgggca gctacgaagc
     5341 cggtaagagt tatacgatta aattgaatgt cgacactaag atgcgttact ataccgtgca
     5401 aatcaattca ggccgcgaag tagggcgtat tttttacgca cctgcggcca gcctcgaaag
     5461 ggtagtgttc agaacaggag aaccacgtct gcatcctaca ccagaaactc cggccgaccg
     5521 ttacactgat ttgccggctg cagatgccgt cgatcccgag gcctgtttcc gtatttactc
     5581 actggaaacg gctcccgcta ccggtaataa tcaataatga atatatttct tatgaaaaaa
     5641 caattagtcg ccttgctgtt aatcggcttt acggtccttt cgtttgcctc ctgtcaggcg
     5701 aaaaccaaaa cacccaaatc ggccaatatt ctcaaagcca aagattttaa acattacgtt
     5761 gattatttta accgcatgga agatgagccc atcgttcagg ccatacctaa tgcggagtcc
     5821 tgggactgga tgcaggccaa tattcccttg ttcagttgtc ctcaggataa tttcgaggaa
     5881 atgtggtact tccgttggtg gtcgttgcgc aagggtattc gcgaaactcc gcttggtttt
     5941 gttatcaacg aatttttggt ggaacgttct tatgccgata agtacaatat gatttcctgt
     6001 gcgttgggac atcatattta tgaaacccgc tggctgcacg attccaaata ccttgatcag
     6061 tatttgcatg tgtggttcag gggtaacgaa ggggctccca tgaaaagatt gcgcagtttc
     6121 agttcatgga ctgcctatgc cgtgtacaat aggtatcttg ccgacggtaa caaagagtat
     6181 atgctcgatt tgttgcccga cctggaaaag gaataccgag actgggaagg cgatcaccgt
     6241 ttgcccgacg gcatgtactg gcagggcgat gtgcaggatg gcatggaaga gtccgtcagc
     6301 gggggacggc gcatcaagaa tgcccgtccc accattaata gttatatgta tggaaatgcc
     6361 ctcgctttgt caaaaatgtt cagcctaagc ggagagacgg ccaaagctgc cctttttgca
     6421 gccaaagccg ataccataaa aaaaatggta caagacaact tatggcatcc cgaaaggaaa
     6481 ttttttgaaa cacgttgggt aaacggccag ctggcgcaag ctcgcgaagc catcggttat
     6541 attccttggt attttaattt gcccgaccta gggaaaaatt acgaagaagc ctggttgcag
     6601 gtcaaagatc cggagggctt tttagctcct tacggtctga ctaccgccga aagacggcat
     6661 cctgaattcc gcactcgcgg agtgggtcaa tgcgaatggg atggggcggt ttggcctttt
     6721 gccagctcgc agacaatgac ggctatggcc aatgtgctca acaattatga gcaaaattac
     6781 gtggacaaag ccacctacta tgaattgatg aatctgtatg tggaatctca gtaccataga
     6841 ggccggccct atataggcga atacctggac gaagttacag gctattggtt gaaaggcgat
     6901 caattgcgca gccgctatta caatcattcc acttttgccg atctgatcat tacgggcttg
     6961 atgggattac gtccgggaga agataatata ttggtagtta atccgctgat tccccaggaa
     7021 caatgggatt ggttttgttt ggataatttg atgtaccatg ggaagaatat agccgtcgtg
     7081 tgggataaat ccggaaagaa attcaataaa ggccaaggtt tacttatctt ttgcaatggc
     7141 aaaaaagtag ctcaaaaacc aactattgaa aaaatcactg ttgatatttc accgtactaa
     7201 gctaaaccac ccggacaatt ttgtgttaaa acggtttagt attacatttg tcagcctaac
     7261 actttgatta tggcgtttaa gaaaaaagta tctctgaaag acatcgcgca agccgttgga
     7321 gtgtcgtccg ctttggtatc gatggtgctc aacggcaagg caaaacaata tggaatcggt
     7381 gaggaggcca ctcgtaaagt gttggccaag gccaaggagt taaactatca acccaatatc
     7441 atggccagag gtcttaggag cggtagaagt cgtctccttg gcttggtggt ggccgatatc
     7501 tccaatcctt tttttgccac tctcgcccgc gaaatagaac gtgctgcctc tgaaagaggc
     7561 tacacggtca tttacggcag ttccgatgaa aagcccgaac atttgtcgaa gcttatgaat
     7621 actttggcca atcagggagt ggatggaatt attgtggttc cctgtgaaaa ttcagaagaa
     7681 ctcattgccg atttatacct
//
LOCUS       DAOSGL010000073         7586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8372_length_7586_cov_4.102510, whole genome shotgun sequence.
ACCESSION   DAOSGL010000073 DAOSGL010000000
VERSION     DAOSGL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8372_length_7586_cov_4.102510"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKY68_03615"
     CDS             <1..642
                     /locus_tag="PKY68_03615"
                     /inference="COORDINATES: protein motif:HMM:NF028352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HOH24157.1"
                     /translation="NSVLQCGPDVLPAGSAAFWEAYLEEQIKRGLILAAGINYRPHTQ
                     RKKPPVPSMHHFLMICGSAHQHSLDYDEYLRKQGVTLMEMPPELSSEQEPDPAAPAAW
                     ANACVQAWKKEPRLALRIASKPISYENSAHKLKARFTDALQLILQQIKPAELFIEGGA
                     TAASLLNRLNWTTFKPVDELAPGVVRLQLMPEPSLFITLKPGSYFWPEFLFNS"
     gene            646..1887
                     /locus_tag="PKY68_03620"
                     /pseudo
     CDS             646..1887
                     /locus_tag="PKY68_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
     gene            2300..3691
                     /locus_tag="PKY68_03625"
     CDS             2300..3691
                     /locus_tag="PKY68_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24158.1"
                     /translation="MKKIWLTALLCISVYAYSQQNSDNRFQRPLKEVLTDLQAQFGVR
                     LRFSEELVRDKVLDYADWRIHPWSLEESLNNVLLPFDYKFVKESSTSYEIKAFEYARR
                     TADFGRDFLDYLSSRYADLPAWEARKALLKKEMKIAAGLDPLPRSPGTKPILGPKRKY
                     EGYYVQNIALEILPGVFCTGSIYHPIGNKGKCPIMLNPNGHFGDGRYRADQQLRCAMQ
                     ARMGIIAVSYDLFAWDEQLLQFDSRSHRSSMAHTIQSLNAIRMLDYLCALKEADLSRV
                     GITGGSGGGSQTMFITAIDERITLSMPVVMTSSYFVGGCPCESGNPIHLCGGRTNNAE
                     ITAMCAPRPLLVVSDGKDWTKDVPDLEFPYIRSIYALYGKEDLAENAHFGDEGHDYGF
                     SKRQATYTFIEKHWNLNTKGLKNAAGQWDESEVTVEPYPSLKVFGADGKGLPAHAVKG
                     MDELYRIFEAAKQ"
     gene            3753..5531
                     /locus_tag="PKY68_03630"
     CDS             3753..5531
                     /locus_tag="PKY68_03630"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH24159.1"
                     /translation="MKKQHQLQGNQNNLKYNKSNSKDTKKIILKTIKDKTMVKYRIFA
                     LCLIVTGLFYACQSPEIDLQAENLKLDHCGGEGARQLCLSWTMNASSPRGLNQQAYRI
                     RIASRPELLEQNSPDIWDSGKVSSSKNYDVPYKGKALERDTVYYAQIRIWTNRGKSLW
                     SKQQNLVLPAEGDLLPAAASINRFDTYDLKLSDLVQAYKTATLEKYKHRPQDLENMSA
                     ADCWAESYLIDNRTFYLQWMDRLLAAQAEDGSLPLSAQQEPQEVFDLAHMLLLQYNET
                     AHLKKHFPAMEKWLDFLWKNYGSQEYILTADGEGHTLLPAEKCSLDGKEYTTSAALIS
                     TAYFYRLCVLMEKFSSHLQYRDKAAHYADWAGKLRKAYNGRFFNEEKQYYENNTPTAN
                     LLSLSLGFCEDKRAENVFKRIEQSIAIEHYGKAACGEFGKRYLYRTLSAWGRSDLSYR
                     LGAELLEVEGDFILWFFEHLCGLKSLEEGFHCFSLDAVFPPGIYFAKTRMETRGGIIS
                     SSWIKTFDEFVWQVIVPVNTRARLRYPAAAVKDIYDDGRVVDQVEGIQILGRENGNIL
                     MELGSGDYFFSSKLADIKPGPRRNNE"
     gene            5561..7468
                     /locus_tag="PKY68_03635"
     CDS             5561..7468
                     /locus_tag="PKY68_03635"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOH24160.1"
                     /translation="MKTKSMLLTMGLLLSFTACRQGAEWKKGILIDEFVYEKTSFPEC
                     HSASLAETPEGIVLTFFGGTKERHPDASIWICRNENGVWTEPEKAADGVVNDTLRYPC
                     WNPVLYQVPGEELLLFYKVGPSPGSWWGCMKRSFDNGKTWSAEEKLPEGIIGPVKNKP
                     VVVDGKLFCPSSTENRGWRLHIDVTEDKGKTWIQIGPLNDPAEWLAIQPSILFHKNGD
                     LQILCRTRNRLVGSTWSTDRGRTWSQLEGVGLPNNNSGTDAVTLKDGRHLLVYNHVEP
                     PAGETKGARTPLNVALSKDGKTWYASLVLEDSPISQYSYPCVIQSSDGFVHIAYTWRR
                     QRIKYVKIDPAKLKMKEIENGIWPGKETAQAVEGAGSTPFPISVCDWMILKRQKLGAI
                     TLAREIGCDGLEIDMGGLGNRPTFDNKLKDPEVLAEFKRTAQVSGIRFSSVAMSGFYA
                     QSLVDREDFITPVQDCIDVMKGLGVGVAFLPLGVKGDLVQYPELRPAIVERLRVIGRM
                     AEQEKLVIGIETSLDAEGDKALLQEIGSPGIKIYFNFARALQSGKDLHRELRLLGDDI
                     CMIHCTNKDSVWLENDPMIDMPAVKQTLEEIGYRGWLVMERSRDASQARNVRGNYGAN
                     CAYLKSVFQPE"
BASE COUNT         2084 a         1809 c         1862 g         1831 t
ORIGIN      
        1 aacagtgtcc tgcaatgtgg cccggacgtc ttaccggccg gcagtgccgc tttttgggag
       61 gcctatctcg aggagcaaat caagcggggc ctgatccttg ccgccggtat caattaccga
      121 ccccatacac aacgtaaaaa gccgcctgtt ccttcgatgc accattttct gatgatctgt
      181 ggcagcgctc atcaacacag tctggattac gacgaatatt taagaaagca gggggtaact
      241 ctgatggaaa tgcccccgga attgagttcg gagcaggaac cggatccggc cgctccggct
      301 gcctgggcga atgcctgtgt gcaagcctgg aaaaaagaac cccgtttggc cctaaggatt
      361 gcctcaaaac caatcagcta cgagaacagc gcccacaaac tcaaagctcg ttttaccgat
      421 gccttgcaat tgattttaca gcaaatcaaa ccggccgaat tattcatcga aggaggtgcg
      481 acggctgcga gtctgctgaa ccggctcaac tggacgacat ttaaaccggt ggacgaattg
      541 gctccgggag tggtccgctt acaattgatg cctgaacctt ctctttttat aaccttgaaa
      601 ccgggcagtt atttctggcc ggaattctta ttcaattcat agacaatgaa aaatctgatc
      661 agcttggcat ggcttctttt attgcctttt tacgcggaag caactactgt caacgcactc
      721 aaagccggcg caaaaaacga tggcaaaaca ctgaatacca atgctatttc taaagcaatc
      781 gataaactga gttcccaagg tggcggcacc ttgtattttc cttcgggtac ctatctgacc
      841 ggtcccataa ggctccaaag caacattacg ctcgatttgg atgccggagc cgtgttgtct
      901 ttttcggatc gtttcgactt atatatgcct tacgtcgaaa tgcgttacga gggagtggtg
      961 atgaagagct ttcatcctct gatttatgct tacgaagccg aaaacatcac tataaagggc
     1021 agaggaagac tcgaaggtaa cggccgggct tggtgggaag agacctggcg actggaaacg
     1081 gcgttctaca agggagaatt gcaagacgcc gaaccgacca aataccagca agcctggctg
     1141 gatgccaatc ccggtttcag tgtcgaaccc cagtcggact ggaaaaacac cttggccagg
     1201 cattggttca gaccgccttt tattcaattg tacaaatgca aaaacatttt gatagaaggt
     1261 ctgcacatcg ctaattctcc tttctggacc gtaaatccgg aattttgcga taatctcacc
     1321 atccagggcc tgaccatcgt aaatccgaat tcccccaaca ccgacggtat caaccccgaa
     1381 tcctgtacca atgtgcatat ttccaattgc catatcagcg tcggcgacga ctgcatcacc
     1441 atcaaatcgg gtcgtgacct gcaagggcgt caatacgccg ctccctgcga aaacatcacc
     1501 atcaccaatt gcaccatgct ttccggtcat ggtggcgtgg ttattggcag tgagatgtcg
     1561 ggagatgtcc gcaagatcac catcagcaat tgcgtgttcg acggcaccga ccgtggtatc
     1621 cgcttaaagt caacccgggg caggggaggc atagtcgaag aaatccgcgt gagcaatatt
     1681 gtgatgaaga acattcaaaa agaggccatt attctaaacc tgttttacag cagggtgcct
     1741 caggagcccg tgaccgaaag aactcccatt ttcagaaaca tccatatttc gggcatgacc
     1801 ggctcggagg tgaaatcggc aggatcgatt ctcggcattc ctgaaatgcc tatttccaat
     1861 atcagcatag aagacgtgaa tatccaggca gaaagcggtt tcagtgttca ggatgcccgg
     1921 gacattcaat tgcgcaatat atgcatcgac acccaaaaag ggccgattgt acaatgcaag
     1981 gatgcagccg aattgtattt gtccaatata cgaagctcca aacccttggc ggaggccgct
     2041 ttgctaagga tggaaaacgt cagcgacgtc tttattgaag gctgtttccc cttgcccgga
     2101 agcaaagctt ttttggaatt atccggtgcg gaaagcagca gggtgatatt gaaaaataat
     2161 tttgttgaac gcattgaaca accgtacctt attcatgaaa tggtcgattc tgccgcattg
     2221 gtttattgat atgaaacatt cctgacgtat tctacgacca agcgaatgaa acagaagtgc
     2281 taacattaaa aattcacgta tgaaaaaaat ctggcttaca gctttattgt gtattagtgt
     2341 ctacgcctat tctcagcaga attccgacaa tcgctttcaa cgtcccctca aagaggtgtt
     2401 gacggattta caggcgcaat ttggagtgcg tctgcgtttt tccgaagaac tggtcaggga
     2461 taaagtgctc gattacgccg attggcgcat tcatccctgg agtctggaag aaagcctgaa
     2521 caacgttttg ctgccttttg actataaatt tgtcaaagaa agcagtactt cttacgaaat
     2581 caaagccttc gagtatgcgc gcagaacggc tgatttcggc agggattttt tagattacct
     2641 gagtagccgg tacgccgatt tgcccgcctg ggaagctcgt aaggccctgc tcaaaaaaga
     2701 aatgaagatt gccgccggcc tggatccgct gccccggagt cccgggacca agcccatcct
     2761 gggccctaaa cgtaaatacg aaggctacta cgtgcaaaat atagccctgg aaattttacc
     2821 cggcgttttt tgtacgggtt caatatacca tcccattgga aacaaaggca aatgtcctat
     2881 tatgctcaat cccaacggtc attttggtga tggccgttat cgcgccgacc aacaactgcg
     2941 ctgtgccatg caagccagga tgggcatcat tgcggtcagt tacgatcttt ttgcctggga
     3001 tgaacagttg ttgcaattcg acagcagaag ccatcgcagc agcatggccc acaccataca
     3061 aagtctgaac gccattagaa tgctcgatta tctctgtgct ttgaaagaag ccgatctcag
     3121 cagagtgggg ataaccggcg gatcgggcgg cggcagccag accatgttta ttacggccat
     3181 cgacgagagg attaccctga gtatgccggt agtgatgact tcttcttatt ttgtgggcgg
     3241 ctgtccctgc gaaagcggca atccgattca tctttgcgga ggcagaacca acaacgccga
     3301 gataaccgcc atgtgtgctc cccgtccctt acttgttgta tccgacggta aagattggac
     3361 caaagacgtt ccggacttgg aatttcccta tattcgcagt atatatgctt tgtacggcaa
     3421 ggaagatctg gccgaaaatg cccattttgg agacgaaggt cacgactacg gcttttcgaa
     3481 acgtcaggcg acctatactt ttatagaaaa gcactggaac ctgaatacca aaggattgaa
     3541 gaacgccgct gggcaatggg atgaatccga ggtaacggtg gaaccctatc cctccctgaa
     3601 ggttttcggg gcagacggca aaggcttgcc ggcccatgcc gtcaaaggga tggacgaatt
     3661 ataccggatt tttgaagcag ccaaacaata aacaacttca aggacagcaa caactccagg
     3721 gacaacaccg accccaagga tagcaaaacc aattgaagaa acaacaccaa ctccagggaa
     3781 accaaaacaa tttaaagtac aacaaaagca actctaagga taccaaaaag atcatcctca
     3841 aaacaataaa agataaaact atggtaaaat accggatatt cgctctttgt ttgatagtta
     3901 ccggcttgtt ttatgcctgt caatcgcctg agatcgattt gcaagccgag aatctcaagc
     3961 tggaccactg cggcggggaa ggggcccggc agctttgcct gagttggacg atgaatgcat
     4021 cctctcccag aggattgaac caacaagcct accgtatacg tattgccagc cgtcctgaac
     4081 tgctcgaaca aaattcgccc gatatttggg attcgggtaa agttagcagc tcaaagaatt
     4141 acgatgttcc gtataaaggt aaagccttag agagagatac cgtctattat gcccaaataa
     4201 ggatatggac caatcggggc aaaagtctct ggtccaaaca gcaaaacctg gttttgccgg
     4261 ctgaaggaga tcttctcccc gctgctgcat ccatcaatcg cttcgacacc tacgacctga
     4321 agttgagcga cctggttcag gcctataaaa cagccactct cgagaaatac aagcatcgcc
     4381 cccaagactt ggaaaacatg tccgccgccg actgttgggc cgaaagttat ctgatcgata
     4441 accgcacatt ttatctccaa tggatggatc gcttgctggc tgctcaagcc gaagacggga
     4501 gcctgccttt gtctgcgcaa caagagccgc aggaagtttt cgatcttgcc catatgttgt
     4561 tgcttcaata caacgaaacc gctcatctga agaaacattt tccggcgatg gaaaaatggc
     4621 tggacttttt gtggaaaaac tacggaagcc aagagtatat actgacagcc gatggagagg
     4681 gacacaccct gttgcctgcc gaaaaatgta gtctggatgg aaaagagtac accacctcgg
     4741 ctgctttgat ttcgacggcg tatttttatc gactttgtgt gttgatggaa aagttctctt
     4801 ctcacttaca gtatcgggat aaagccgctc attatgccga ttgggccggc aagcttcgta
     4861 aagcctacaa cggccgtttt ttcaatgaag aaaaacaata ctacgaaaac aataccccga
     4921 cggccaattt attgtcccta agcttaggat tctgtgaaga taagagggcg gagaatgtat
     4981 tcaaacgaat agaacaatcc atagccatag agcattacgg aaaagcagct tgcggtgaat
     5041 ttggcaaaag atatttgtac cgcaccttat cggcctgggg cagatccgat ctgagttaca
     5101 gacttggggc tgagctcctg gaggtcgagg gcgattttat tctgtggttt tttgagcatc
     5161 tatgcggttt gaaaagtctg gaagagggct tccattgttt cagcctcgat gccgtcttcc
     5221 ccccgggaat ttattttgca aagacccgta tggaaacccg tggaggaatt atttccagct
     5281 cctggataaa aaccttcgat gaatttgttt ggcaggtgat cgttcctgtc aataccaggg
     5341 ccagactccg ctatccggct gctgccgtca aagacattta cgacgacggc cgggtagttg
     5401 atcaggtaga gggcattcaa attcttggtc gggaaaacgg aaatatactt atggaactgg
     5461 gctcgggcga ttattttttc tccagtaaat tggccgacat taaaccaggc ccgcgtagga
     5521 acaatgaatg atttatatcc aacctttaat tttatattgt atgaaaacaa aaagtatgct
     5581 attgacaatg ggccttttgc tcagctttac ggcctgccgg caaggagctg aatggaaaaa
     5641 gggaatcctt atagacgaat ttgtttacga aaaaacttcc tttcccgaat gtcactccgc
     5701 ttcgttggca gaaactcccg aaggtattgt gttgactttt ttcggcggga ctaaagaacg
     5761 ccaccccgat gccagtatat ggatatgccg taacgaaaac ggtgtttgga ccgaacccga
     5821 aaaagcggcc gacggagtag tcaacgatac attgagatat ccttgctgga atcctgtgtt
     5881 gtatcaagtt cccggcgaag agttgctgct gttttacaag gtcggcccca gtcccggatc
     5941 ttggtggggt tgtatgaaac gttccttcga caacggtaaa acctggtctg ccgaggaaaa
     6001 actgcccgag ggcattatcg gcccggtcaa aaacaaaccg gtggtggtgg acggaaaatt
     6061 attctgtcct tcgagcaccg aaaaccgcgg atggagactg cacatcgatg ttaccgaaga
     6121 caaaggcaaa acctggatac agataggccc tttgaacgat ccggcagaat ggctggctat
     6181 tcaaccaagt attttattcc acaaaaacgg agatttgcaa atactctgtc gtacccgcaa
     6241 ccgcctggtg ggttctacct ggtcaaccga ccgtggccgg acctggtcgc agttggaagg
     6301 tgtaggtttg cctaacaaca attcgggaac ggatgccgtt actttgaaag acggccggca
     6361 tctgctggtt tataatcacg tggaacctcc tgccggtgag acaaaaggag cccgaacccc
     6421 tctgaacgta gctttatcga aagacggaaa aacctggtac gcttcgctgg tgctggaaga
     6481 ttcacccatc agtcaatatt cctatccctg cgttattcaa tcctccgacg gtttcgtgca
     6541 catagcttac acctggagac gccagcgcat taaatacgtc aaaatcgacc ccgccaaact
     6601 taaaatgaaa gaaatagaaa acggtatctg gcccggcaag gaaacagccc aggccgttga
     6661 gggtgcaggc tctacccctt ttcccatttc ggtttgcgac tggatgatat taaagcgcca
     6721 gaaactcgga gccatcactc tggccagaga aataggctgc gacggccttg aaatagacat
     6781 gggtggcctg ggtaatcgtc ccacttttga caacaaactc aaagatcccg aggtgctggc
     6841 tgaattcaaa cgtaccgcac aggtgagcgg aattcgcttc tcctcggtgg ccatgtcggg
     6901 tttttacgct caatcactgg tagatcgcga agattttatc acaccggtgc aggattgtat
     6961 cgacgtaatg aaaggtctgg gtgtaggagt agcttttttg cctttgggtg taaaaggaga
     7021 cctggtgcaa tatcccgaat tgcgccccgc tatagtggag cgcctgcgag ttatcggccg
     7081 gatggccgaa caggaaaagc tggtcatcgg tatagaaacc tctttggatg ctgagggcga
     7141 taaagcatta ttgcaggaaa tcggcagtcc ggggatcaag atttatttca atttcgcaag
     7201 agccctgcaa agcggcaaag atttgcatcg ggagttgcgc ttgttgggtg acgatatatg
     7261 tatgatacat tgcaccaaca aagacagcgt atggctggaa aacgacccta tgatcgatat
     7321 gcctgccgtc aaacagacac tcgaagaaat cggttaccgg ggctggctgg taatggaacg
     7381 ttctagggat gcaagccagg cccggaatgt ccgtggaaat tacggggcca attgtgccta
     7441 tcttaagtcg gtttttcaac cggaataata tggtttgaac ccggtttgat ccgaatcaat
     7501 cagaaataaa cgtattttat agtgaggcta aaaacaacat agttatgatc aaaaaatctt
     7561 tcattgtatt agctctgtta tgtagc
//
LOCUS       DAOSGL010000074         7498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8510_length_7498_cov_3.977832, whole genome shotgun sequence.
ACCESSION   DAOSGL010000074 DAOSGL010000000
VERSION     DAOSGL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8510_length_7498_cov_3.977832"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PKY68_03640"
     CDS             complement(<1..904)
                     /locus_tag="PKY68_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOH24161.1"
                     /translation="MNRQLLTKTIILCLIGLMTGCNAPKATSFEIGDKQFLLNGEAFV
                     IKAAEMHYTRIPRPYWEHRIKMSKALGMNSICLYIFWNIHEQEPGVFDFEGQNDVAEF
                     CRMAQKEGMYVIIRPGPYVCAEWEMGGLPWWLLKKEGIALRSQDPYYLERVEIFMNEV
                     GRQLAGLQINRGGNIIMVQVENEFGSYGTDKPYVEDIRDIVRAAGFTDVPLFQCDWNS
                     NFQNNALDDLLWTINFGTGANIDNQFRELKRLRPNTPLMCSEFWSGWFDRWGSPHETR
                     SAEELVKGMQEMLEKDISFSLYMTH"
     gene            complement(1029..1802)
                     /locus_tag="PKY68_03645"
     CDS             complement(1029..1802)
                     /locus_tag="PKY68_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOH24162.1"
                     /translation="MKILILGAGKMGSFFADVLSFEHETAVYDIDPRKLRFIYNTCRF
                     KELEEIREFAPELLINAVTVKYTIEAFKTVLPLLPASCIISDIASVKNDLEDFYAGCG
                     HRFVSTHPMFGPTFASLNNLSSENAIVITESDHLGKTFFLDLYRKLGLNIFEYSFKEH
                     DKTVAYSLSIPFVSTLVFSAVMKHQDAPGTTFKKHLAIAHGLMNEDDFLLQEILFNSE
                     TPAQLELIRSELKQILEIVKNRDAQRMKSYLEKIRNNIR"
     gene            complement(1799..2887)
                     /locus_tag="PKY68_03650"
     CDS             complement(1799..2887)
                     /locus_tag="PKY68_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOH24163.1"
                     /translation="MQFNRQLFPDLDLTRPIVISGPCSAETEEQVLATAGQLADKGIR
                     IFRAGIWKPRTRPGAFEGIGTKGLAWLKLVKEQTGMYTCTEVASTQHVYEAVKYGVDM
                     LWIGARSTVNPFLMQEISDAVQGLDIPVLVKNPVNPDLELWVGAIERLYKAGIRKIGV
                     VHRGFSSWDKQIYRNAPHWHIPIELRRRFPDLLMFCDPSHMGGKRELISPLSQQAMDL
                     NYDGLFIETHYKPEAALSDSGQQITPDVLQYILKTLVVRQGGKPDAEKLSAYRAEIDE
                     YDDQLVELISKRMKVSKLIGHYKKENNIQVLQAARYNEIIEERVKQAAGLGIKSDCMQ
                     KILEAIHEESVRLQIEIMNMDNSKTSEK"
     gene            complement(2915..4126)
                     /locus_tag="PKY68_03655"
     CDS             complement(2915..4126)
                     /locus_tag="PKY68_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH24164.1"
                     /translation="MQKEIKPAKRLEGINEYYFSSKLREVAMLNAQGKDIISLAIGSP
                     DLMPRAEVVETLCKEAAQKTAHGYQPNIGRKELRSAFAAWYKEWYGVELDPDREILPL
                     IGSKEGVMHVSMTFLNPGDAVLLPNPGYPTYRAVCKLLQAEVLEYELDEQNGWQPDFE
                     ALEKMDLSRVKLMWVNYPHMPTGAPAQKSTFEKLVDFGRRHGIVVCNDNPYSFILNNH
                     PLSILSVPGAKDCCLELNSLSKSHNMPGWRIGMLAGNPEFVSWVVRLKSNVDSGQFLP
                     MQLAAVKALQSERSWYKELNRRYSSRRVLIEELMCSLHCTFDAGQEGIFLWGRIPDDY
                     TDAAELCDELLYKARVFVAPGFIFGSKGNRYIRISLCSPEEQIREARRRVCNYLHKQK
                     CSSAGKPVLTS"
     gene            complement(4362..6266)
                     /locus_tag="PKY68_03660"
     CDS             complement(4362..6266)
                     /locus_tag="PKY68_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOH24165.1"
                     /translation="MTIYHDILKQYWGYEDFRELQLEIIESVAAGKDTLGLMPTGGGK
                     SLTFQVPTLAMEGICLVITPLIALMRDQVERLKSMDIRAACLHSGMTRQEIQIVLDNC
                     LYGDFKFLYVSPERLGSDLFLQRALGLPVNLLVVDEAHCISQWGYDFRPSYLNIARVR
                     EYFPSIPVLALTATATPEVVEDIQDKLHFAAPNVFKVSFERKNLAYVVRETQDKDKAL
                     LQVLKGVDGTSIVYVRNRAKTKQIANFLKGEGVEAAHFHAGLNDQTKQERQAAWKNGT
                     CRVIVATNAFGMGIDKPDVRTVIHMDLPDSLEAYFQEAGRAGRDGQKSYAVLLYHKSD
                     STLLKKRLSDNFPNKKYIRKVYEALGNYYQLAVGSGQGMNFPFDLQDFCKKFHLNMLQ
                     AYNGLKILELAGYLELTDEQDSTSRLYFLSSREDLYHLPRSGNKTDRVLQVLLRSYTG
                     LFSDFVRIQEEVLASRLQMTGEEVYESLLYLSRQGVIHYIPRKKTPFICYTQPREESE
                     RLHIGPEVYEERKKRYVERIQAMQAYAAEDQPCRSKRLLQYFGQKRVSNCRQCDVCLR
                     KQQEGLRQYEFEDISRDMLAALENSNPDLEELIDSLPYDSNKSLKVFRFLCDRGDIIQ
                     REHRIFRKTD"
     gene            complement(6341..>7498)
                     /locus_tag="PKY68_03665"
     CDS             complement(6341..>7498)
                     /locus_tag="PKY68_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH24166.1"
                     /translation="CQYPYKELSGCGVGFKFMQAFAQNNGIPFDRLLPLLDLVVVSIA
                     SDIVPITGENRILAFHGLKQLNTKPSVGLKAIIQLSGLVGKEITISDIVFKIGPRLNA
                     SGRIQSAGEAVELLVTKNLRFAKEKSLQIDQYNQTRKDLDRNITEEANAALKACTNLD
                     DRKGIVIFNEEWHKGVIGIVASRLSEEYYRPAVVLTRSDDLATGSARSVPGFDIYKAI
                     DSCRDLLENFGGHTYAAGLSMKMEKVEEFTRRFEQYVADTISLEQLTPTIDIDAEIKF
                     QEITPKFFRVLKQFNPFGPDNNKPVFSTRRVFDYGTSRLVGRDQEHLKLELVDSHSEN
                     IMNGIAFGMHAHNDHVKSFNPFDICYNIEENYYNGNSSIQLLVRDIKVVED"
BASE COUNT         1938 a         1789 c         1693 g         2078 t
ORIGIN      
        1 cgtgagtcat gtagagactg aaggaaatgt ctttttcgag catttcctgc atacctttaa
       61 ccaattcttc ggcactgcgg gtttcgtgcg ggctgcccca gcggtcgaac cagcccgacc
      121 agaattcgct gcacataagc ggcgtatttg gacgcaggcg tttcagctca cgaaattgat
      181 tgtctatgtt ggcacctgtg ccgaaattga tggtccacaa caaatcgtcc agggcattgt
      241 tttggaaatt ggaattccag tcgcattgaa acaagggcac atcggtaaat ccggcagccc
      301 ttactatatc gcggatatcc tccacatagg gtttgtcggt accataggag ccgaactcgt
      361 tttctacctg taccatgatg atattaccac ccctgttgat ttgcagaccg gccagttgac
      421 ggcccacttc gttcatgaag atttccactc tttccaaata atagggatct tgcgaacgca
      481 aggctatgcc ttctttcttg agcagccacc aaggcaaacc tcccatctcc cactctgcac
      541 aaacataggg tccgggcctg ataatgacat acatgccttc tttttgcgcc atacggcaaa
      601 attcagccac atcgttttgc ccttcgaaat cgaaaactcc gggttcttgt tcgtgtatat
      661 tccaaaaaat atacagacat attgagttca tgcccaaagc tttgctcatt ttgatgcgat
      721 gttcccagta aggcctgggg atacgggtgt agtgcatttc ggcagcctta atcacaaagg
      781 cttctccatt aagcaggaat tgcttgtctc ctatttcgaa gctcgtggct ttgggagcat
      841 tacatcctgt catcaggcca atcaggcata gaatgattgt tttcgttaaa agctgtctgt
      901 tcattgttct ctttttaata gtaacacaaa atgccaccgg tctgcccaaa ctcaggaata
      961 agccgaggcg accaatggcg cgaaaataca aaaaatacta agtacctaaa aatttgacca
     1021 agggaaactt agcgaatgtt gttgcgtatc ttttccaaat aacttttcat cctctgagca
     1081 tctctgtttt tgactatttc cagaatttgc ttcaattcgg agcgaatcaa ctcaagctga
     1141 gcgggagttt cggaattgaa gagtatttcc tgcaaaagaa agtcgtcctc gttcatcagg
     1201 ccgtgggcta tggctaagtg ttttttgaag gtagttcccg gagcatcctg gtgtttcatg
     1261 accgccgaaa aaaccagtgt tgatacaaag ggaatagaca gagaataagc aacggttttg
     1321 tcgtgctctt tgaaggaata ctcaaaaata ttcaatccga gcttgcggta caaatccagg
     1381 aaaaaggtct tgcccaggtg atcgctttcg gtaataacga tggcgttctc gctcgaaaga
     1441 ttgttcaggc tggcaaaggt cggcccaaac atggggtggg tcgatacaaa gcgatgaccg
     1501 caaccggcat aaaagtcttc caggtcgttt ttgacactgg caatatcgct gatgatgcaa
     1561 gaagcgggca acaaaggcaa aacagtcttg aaagcctcga tagtgtactt caccgttaca
     1621 gcgtttatca gcaattccgg agcaaattcc cggatttctt cgagttcttt gaagcggcag
     1681 gtattgtaaa taaaacggag tttccgggga tctatgtcgt aaacggccgt ttcgtgctca
     1741 aaactaagga cgtcggcaaa gaaactgccc attttgccgg cacccaatat caggattttc
     1801 atttttccga agttttcgaa ttgtccatat tcatgatttc aatctgcagc ctgaccgatt
     1861 cctcgtgtat ggcttcgagt attttttgca tacaatcgct tttaataccc aaaccggcgg
     1921 cctgtttcac tctctcttcg ataatttcgt tgtaacgggc ggcttgcaat acctgtatat
     1981 tgttttcttt tttgtaatgc cctatcagtt tggatacctt catgcgtttg ctgataagtt
     2041 ccaccagctg atcgtcgtat tcgtcgattt cggcccgata ggcagacagc ttttctgcgt
     2101 cgggtttgcc tccctgtcgt acgaccagtg ttttcagaat gtattgcaac acatcggggg
     2161 taatttgctg accggaatca cttagcgccg cttcgggttt gtagtgcgtt tcaataaaca
     2221 aaccgtcgta attcaggtcc atggcctgct gactcaatgg agagatcaat tcccgcttgc
     2281 ctcccatgtg gctgggatcg caaaacatca acaaatcggg gaaacgccgg cgcaattcta
     2341 tggggatatg ccaatgcgga gcattgcggt agatttgttt atcccaggaa ctaaaacctc
     2401 tgtgaacaac tccgatcttg cgtattcctg ctttgtaaag ccgttcaatg gctcccaccc
     2461 acaattccag atcgggattg acggggtttt ttaccaatac gggaatatcc aatccttgta
     2521 cggcatcgga gatttcctgc atcaaaaagg gattgacggt acttctcgcc cctatccaga
     2581 gcatatctac tccatacttg accgcttcgt acacatgctg ggtcgaggcc acttcggtgc
     2641 aagtgtacat accggtttgt tctttgacga gtttgagcca ggccaagcct ttggtgccga
     2701 ttccttcgaa ggcaccgggc cgggtacggg gcttccaaat acccgctctg aaaattcgta
     2761 tacctttatc ggccagttgt ccggctgttg ctaatacctg ttcctctgtt tcggcactgc
     2821 aagggccgga aataacgatg ggccttgtca gatcgaggtc gggaaataac tgtctattga
     2881 attgcatata gtttaagtat aaaatgtata attattaact cgtcaggacc ggctttccgg
     2941 ctgaactgca cttttgttta tgtaaatagt tacagaccct cctacgggct tcacgaattt
     3001 gttcttcggg actgcagagc gaaattcgta tataacggtt gcccttgctg ccaaaaataa
     3061 aaccgggagc cacaaatacc cttgccttgt ataaaagttc atcgcacaat tccgcagcgt
     3121 ctgtataatc atcgggaatt cgcccccaca aaaagatgcc ttcctggccg gcgtcgaagg
     3181 tgcaatgcaa actgcacatg agttcctcta taagaacccg gcgcgaagaa taccgcctgt
     3241 tgagttcttt gtaccagctc cgctccgatt gcagggcttt aacggctgcc aactgcatgg
     3301 gcaggaattg accggaatcc acattgctct ttaacctgac gacccaggaa acaaattccg
     3361 gattgccggc cagcatgcct atgcgccagc cgggcatatt atgcgatttg ctcagagaat
     3421 tcagttccag gcaacagtct ttggctccgg gaacagataa aatactcagg ggatggttgt
     3481 tcaaaataaa actgtaggga ttgtcgttgc aaacaacaat accgtgacgg cggccaaaat
     3541 cgaccagttt ttcgaaggtg cttttttgtg cgggtgctcc tgtgggcata tgagggtaat
     3601 tgacccacat aagttttacc cggctcaagt ccattttttc caaggcttca aaatcgggtt
     3661 gccagccgtt ttgttcgtcg agttcgtatt cgagcacttc ggcctgcagc agcttacaaa
     3721 cggctctata agtcgggtaa cccggattgg gcagcaacac ggcgtctccg ggattcagga
     3781 aagtcatgga aacatgcatc actccctctt tggaacctat caggggaaga atttccctgt
     3841 cgggatccaa ttccacgccg taccattcct tgtaccaggc ggcaaaagcc gaacgcaatt
     3901 ctttacggcc aatattgggc tgatagccgt gagccgtctt ttgcgcggct tctttacaca
     3961 atgtttcaac aacctctgcc cggggcatta aatcggggct gccaatggcc agactgataa
     4021 tatctttgcc ttgagcgttg agcatggcaa cttcacggag tttggacgaa aaatagtatt
     4081 cgtttattcc ctcaaggcgt ttggcaggtt ttatttcttt ctgcatggtt atggatttta
     4141 ttaaataaaa aaatcccgcc caattaggcg ggatcgctat caatatatct ctttatctgc
     4201 tcagattttt ttacaatatt accccgccct taacttttcc agctaaagta aaaataataa
     4261 gagtaaacaa aaagctgagt agccatatgt cttgaataat tcggggcaaa tatagtcttt
     4321 ttttttattt ccaactaaaa aaacattttt ttgtgaaata atcagtcggt tttacgaaat
     4381 atccggtgtt ctctctgaat aatatcgcct ctgtcgcaga ggaacctgaa cactttgaga
     4441 gatttattgc tgtcgtaagg caagctgtct atcaactctt ccaagtcagg attactgttc
     4501 tctaaagccg cgagcatatc ccggctgatg tcttcaaatt catattgacg caaaccttcc
     4561 tgctgtttcc taaggcaaac atcgcattgc cggcaattgg atacccgctt ttggccgaaa
     4621 tattgcagca gtcttttact tcgacagggt tgatcttcgg cggcataagc ctgcatggcc
     4681 tgaattcttt ccacataacg ctttttgcgt tcttcgtaca cttcgggacc aatgtgcagg
     4741 cgctcgcttt cttctctggg ctgagtataa caaataaaag gcgttttttt tcggggtatg
     4801 taatgaatta ctccctgacg ggataaatac aacaagcttt cgtacacttc ttccccggtc
     4861 atttgcagcc tggaagccag tacttcttcc tgtattcgca caaaatcgga aaacaaaccg
     4921 gtataagaac gcagcaatac ctgcaatacg cgatctgttt tgtttccgga gcgtggaaga
     4981 tgatacaaat cttctctgct gctcaaaaaa tacaaacgcg aagtgctgtc ttgttcatcg
     5041 gtcaattcca gataaccggc cagttccagt attttcaagc cgttgtatgc ctgcagcatg
     5101 ttcaggtgga attttttgca aaaatcctgc aaatcaaagg gaaaattcat tccctgaccg
     5161 gaacccacgg ccaactgata ataattgccc agcgcttcat agactttgcg gatgtatttc
     5221 ttattgggaa agttgtcgct gagtcttttt ttaagcagag tgctgtctga tttgtggtag
     5281 agcaacaccg cataggattt ttgtccgtct cgacctgctc ggccggcttc ctgaaaatag
     5341 gcttcgagcg agtcgggcaa atccatgtga atcaccgtgc gcacatcggg cttatctatg
     5401 cccatgccaa aggcattggt ggccacaatc acccggcaag ttccgttttt ccaggcagct
     5461 tggcgttctt gtttggtctg atcgttgagt ccggcatgaa aatgagccgc ctctacgccc
     5521 tctcctttta gaaaatttgc aatttgtttg gttttggccc tgttgcgcac gtaaacaata
     5581 gaggttccgt ctacaccttt taacacctgc aaaagagctt tgtctttgtc ctgagtttcc
     5641 ctgactacat aagccagatt tttacgttca aagctgactt taaaaacatt aggggcggcg
     5701 aaatgcagtt tatcctgtat atcttctaca acttcaggtg tagcagtggc cgtgagggca
     5761 aggacgggta tggatggaaa atactcgcgt acgcgggcaa tattcaggta agagggtctg
     5821 aagtcgtaac cccattgcga aatacaatgc gcttcatcca ctaccagcag atttaccggc
     5881 agacccaggg ctctttgtaa aaagagatcg ctgcccagtc tttcgggaga gacgtacaag
     5941 aatttaaaat ctccgtacaa gcagttgtcc aacactattt gtatctcctg ccgagtcatt
     6001 ccggagtgca aacaggcggc gcgaatgtcc atagacttga gcctctccac ctggtcgcgc
     6061 attagggcta tcaaaggggt gataaccaga caaataccct ccatcgcaag agtaggcacc
     6121 tgaaaagtga gggacttgcc gcctccggta ggcatcaggc ccagggtatc cttaccggcg
     6181 gcaacggact caataatttc gagctgtaat tccctgaaat cctcgtagcc ccagtactgt
     6241 ttcagaatgt cgtgataaat ggtcattatc cggagaattt agctgcgccg gtgcctgaag
     6301 ctcctaacag ttcgaaaggc aaatgaacaa aaacggaatc ttaatcttca acaactttga
     6361 tatctcttac aagcaactga atggaagaat tcccgttgta atagttttct tcaatgttat
     6421 aacaaatatc aaaaggattg aacgacttga catgatcgtt gtgagcgtgc ataccgaagg
     6481 caatcccgtt cattatgttt tcggaatgac tgtcgaccag ttcaagcttg agatgttcct
     6541 ggtcgcggcc taccaagcgg ctggtaccgt aatcaaaaac cctgcgggta gaaaagaccg
     6601 gcttattgtt gtcaggtcca aagggattga attgcttgag caccctgaaa aatttaggcg
     6661 taatttcctg gaactttatt tcggcatcaa tatcgatggt aggggtaagt tgttccaatg
     6721 atatggtatc ggccacatat tgctcaaaac ggcgggtgaa ctcctccacc ttctccattt
     6781 tcatggataa gcccgccgca taggtatgcc cgccaaaatt ttccagcaaa tcacgacagg
     6841 aatcgatggc tttgtaaata tcaaaacccg gcacggaacg agccgaaccg gtggccaaat
     6901 cgtcggaacg agtcaaaacc acggccggac ggtagtattc ctccgacaac ctggaggcaa
     6961 caatgccaat cactcctttg tgccattcct cgttgaagat cactattcct ttacggtcgt
     7021 ccagattggt acaggcctta agggctgcat tggcttcttc ggtaatgttc ctgtccaagt
     7081 ctttgcgggt ttgattatac tggtcaattt gcaagctttt ctccttggca aaacgcagat
     7141 ttttggtaac caaaagttcg acagcttccc cggccgactg aattcgaccg gaagcattga
     7201 gcctggggcc gatcttaaag acaatatcgc tgatggtgat ttctttacct accaatccgg
     7261 aaagttgaat aatagctttt aagcctacac taggtttggt attcaactgt ttgagaccgt
     7321 gaaaggccag aatacgattt tcgcccgtaa taggaacaat atccgaggct atacttacta
     7381 ccaccaaatc gagtaaaggc aacaagcgat cgaaagggat accgttattt tgagcaaaag
     7441 cttgcatgaa cttaaaaccg acaccgcaac ccgataattc cttatagggg tattggca
//
LOCUS       DAOSGL010000075         7445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8577_length_7445_cov_5.041678, whole genome shotgun sequence.
ACCESSION   DAOSGL010000075 DAOSGL010000000
VERSION     DAOSGL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8577_length_7445_cov_5.041678"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKY68_03670"
     CDS             complement(<1..592)
                     /locus_tag="PKY68_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOH24167.1"
                     /translation="MADSKEKLFSEFPPVDTQAWMDTITADLKGADFEKRLVWKTNEG
                     FKVQPFYREEDLSQIKIKEALPGEFPFVRGNKTDNRWLVNQEIVVDKPETANKKALSA
                     LNNGVDSLTFVFSGKTFGNPEMGKLLEGIDPESVEINFRNCFGNNSIVLSLFIDYLKT
                     KKVDLNKVKGSLMGDPFRQCLVFGKAYNAQWEEEVAN"
     gene            complement(753..1655)
                     /gene="miaA"
                     /locus_tag="PKY68_03675"
     CDS             complement(753..1655)
                     /gene="miaA"
                     /locus_tag="PKY68_03675"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOH24168.1"
                     /translation="MKLLVVLTGPTGVGKTRLSLKLAQFLNSPVISCDSRQMYREMRI
                     GTAAPDPKDLEATKHFFIGNLSIHDYYNAARFETEALALLDKLFVEHDVLLMTGGSMF
                     YIDAVCFGIDDMPDVDPQLRKELEETFKKRGLSDLLGQLKLLDPEYYHTVDKKNHKRV
                     LHALEICLMTGKPFSAFHRNTPKKRSFDILKICLNRDRAELYRRIDQRVLLMVQQGLV
                     EEARSLYPYKGLNALNTVGYQELFDHFEDKCSLEEAIGKIQFNTHKYARKQLTWFRKD
                     RDYHWFHPDREQDILDLIKNRLKK"
     gene            complement(1667..2239)
                     /locus_tag="PKY68_03680"
     CDS             complement(1667..2239)
                     /locus_tag="PKY68_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24169.1"
                     /translation="MVYKFRLLSDESDLFCREIMIDSEAYFLDLHNTILESVDYEKNM
                     ITSFFMCDEKWEKELEVTLIEMESSPEYDSWVMGTTRLSEMLDEEGQRLIYVFDNLTE
                     RAFYMELVEIFPTKRLSQPECVFSRGKAPKQNMGFEHDKKEKVSTAVVSEDDSLDDFY
                     GEGDFDPEELDPEGFSDLEGFGDNPSLDDY"
     gene            complement(2275..3057)
                     /gene="lpxA"
                     /locus_tag="PKY68_03685"
     CDS             complement(2275..3057)
                     /gene="lpxA"
                     /locus_tag="PKY68_03685"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOH24170.1"
                     /translation="MNQQTLSCIHPEAQIAPDVVIDPFVFIDKDVVIGEGCHIYSHAT
                     ILSGSRIGKNCRIFPGAVLGGIPQDLKFKGEDTLLEIGDNNTIREFVTLNRGTASKGK
                     TVIGNNCLIMAYCHVAHDCVIGNNVIMSNATNLAGEVVVDDFAVISGGVLVHQFTHIG
                     MHCMVQGGSKVGKDVPPFSMAGRDPLGYCGINSVGLRRRNFTDKQIHTIQDVYRHIYM
                     SGRNVSDALKQIREEMPPSKERDIILDFVENAPRGIIKGYLP"
     gene            complement(3076..4482)
                     /locus_tag="PKY68_03690"
     CDS             complement(3076..4482)
                     /locus_tag="PKY68_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOH24171.1"
                     /translation="MPKQLSLKKPFSVSGKGLHTGLQLTATFLPAEANTGIRFRRIDL
                     EGKPEIRALAEFVEHTERGTVLVYNKVSISTVEHAVSALYAAGIDNCIIEVNGPEIPI
                     LDGSAIHFVEGIEKSGVEKLEAEKDFYIVHSKVEVSDPETGASLIILPYDSFSITTLI
                     SYDSCILSNQYARLNRMEDFGKHIAASRTFVFVREVEQLLKHNLIKGGDLDNALVIYD
                     QEVSQEELDRIADVMNIPHKIVNKLGYINNKPVLYDNEPARHKLLDLVGDIALIGKPI
                     KGHIIAMRPGHKINNQLARLIRKDIIRQEVQAPLIDHRKPALMDTKQIRNLLPHRWPF
                     QMVDKVIEITDSYIIGIKNLTANESFFQGHFPDEPVMPGVLQLEAMAQTGGLFLMNQT
                     KNPEKYITYLVKIEYVRFRKKVVPGDTLVFKVYHIPPISHGMAKMKGYAFVGNELVTE
                     AEMTAMLTKRKRVPVDNE"
     gene            complement(4475..5527)
                     /gene="lpxD"
                     /locus_tag="PKY68_03695"
     CDS             complement(4475..5527)
                     /gene="lpxD"
                     /locus_tag="PKY68_03695"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOH24172.1"
                     /translation="MDFTASQIASFLSGTVEGNPDVLINTFSKIEEGSQGALTFLSNE
                     KYTSYLYTTKASAVLVNKDFKPEKSVSATLIRVENPYEALAKLLQWSEQFKEKFQGID
                     KNSFVDPTAVLGKEVYVGHFSCLSAQVEVGDNSKIYPQVYLGRRVRIGKNCILYPGVK
                     VFDDCVIGDNCILQAGVVIGADGFGFAPQKDGSYTKIPQIGNVVIEDDVEICANTTVD
                     RATMGSTIIRKGVKLDNLIMVAHNVEIGENTVIAAQTGIAGSTKIGSKCMFGGQVGIT
                     GHINIPDGTIVCAQAGIISTVKTNEHPLLGSPAIPSKEYLRSYTLFRKLPELVSELKA
                     TREELEALKQKINNDA"
     gene            complement(5600..6820)
                     /locus_tag="PKY68_03700"
     CDS             complement(5600..6820)
                     /locus_tag="PKY68_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH24173.1"
                     /translation="MGMKQKIINDPVFGFINLPTGFLYQVYQHPYLYRLSRIQQLGLS
                     FLVYPGARHCRFQHITGALHLASEAIRQLRLKGNEITEAEAEGTVAAIMLHDIGHGPF
                     SHVLEPVLFNGLSHEKISLLLMERMNKEFNGKLDMAIAIFKGSYPKKFLHQLISSQLD
                     VDRLDYLRRDSFFTGVVEGNIGSSRIIKMLAVCEDRLVVEAKGIYSIENFLMARRLMY
                     WQVYLHKTSLAAEKMLHNLLHRAKVLKQTGQDLTCSPALSFFLSGKLKPGEISDVALD
                     HFIALDDTDIWSAIKNWQNHPDVVLSTLSRNFLNRKLFKIEISEQETPASRVQEDLQR
                     IALQLNISTEDARYFVSLDKVSSHIYDDADYGIDILYNDGRIRPITQASDILNLEVLS
                     KKVRKYAYSYLRKV"
     gene            complement(6811..>7445)
                     /gene="pyrF"
                     /locus_tag="PKY68_03705"
     CDS             complement(6811..>7445)
                     /gene="pyrF"
                     /locus_tag="PKY68_03705"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOH24174.1"
                     /translation="SCGLEGWKAFEKTVRYIREKDPTQFIIADAKRGDIGNTAEMYAR
                     SFYESLPIDAVTLAPYMGKDSVQPFLSYPGKWAILLALTSNRGSADFQMLEDAQGERL
                     FEKVLRRSSDWGDEFSMMYVVGATQGSLLQDVRKLVPRHFLLVPGVGAQGGSLKEVAR
                     YAMNDDCGLLVNASRQILYASKDEEFAQAARQEALKLQQEMQTYLKALWG"
BASE COUNT         1844 a         1704 c         1730 g         2167 t
ORIGIN      
        1 gattggcaac ctcctcttcc cattgagcgt tgtaggcttt gccaaaaaca aggcattgcc
       61 tgaagggatc gcccatcaac gaacctttta ccttattcag gtcaactttt ttggtcttca
      121 aatagtctat gaacagactc aggactatgg aattgtttcc aaaacaattg cgaaaattga
      181 tttcgacact ttcgggatca atgccttcga ggagctttcc catctcggga ttgccaaacg
      241 tttttccgga aaacacaaaa gtgagggaat ccactccgtt gttcagcgcc gacaaagctt
      301 ttttgttggc tgtttccggt ttatctacca caatttcctg atttaccagc cagcggttat
      361 cggttttgtt gcctcgcaca aaagggaatt ctccgggaag agcctctttg atcttgattt
      421 ggctaagatc ctcttctcga tagaagggtt gtaccttgaa gccttcgttg gttttccaaa
      481 cgagtctttt ctcgaaatca gcccctttta aatcggcggt aattgtatcc atccaggctt
      541 gggtgtcgac cggtggaaat tccgagaaaa gtttttcttt gctatctgcc atgataataa
      601 aatattgatt tttagaatat tataagtgag tccgtttatt tggaataaat attcaataag
      661 acctgcaaag ataggtcttt ttattctgac tgtgaaaaga ataagccttg tttttaaacc
      721 gtataaaccg ggcttttgga accgggaacg gatcattttt taaggcggtt tttgatcagg
      781 tctaaaatat cctgttcccg atccggatgg aaccaatgat agtcccggtc ttttctgaac
      841 caggtgagtt gcttacgggc gtatttatgg gtattaaact gaatcttacc gatagcctct
      901 tccaggctgc atttgtcctc gaaatgatcg aacaattcct gataaccgac agtattcaat
      961 gcattaaggc ccttgtaggg atacaggctt cttgcttctt cgaccaaacc ttgttgtacc
     1021 ataagcagta cccgttggtc tattcgccgg tagagctctg ctctgtcgcg gttgaggcaa
     1081 attttcaata tatcgaaact ccgttttttg ggtgtattgc gatgaaaggc cgaaaagggt
     1141 ttgccggtca tcaggcagat ttccagggca tgcaacactc ttttgtggtt ctttttgtct
     1201 actgtgtggt aatactccgg atcgaggagt ttgagctgtc ccagtagatc actcaggccc
     1261 ctttttttaa aagtctcttc cagttctttt ctgagctggg gatcgacatc gggcatatcg
     1321 tcgatgccaa agcacacggc atcgatataa aacatggaac cgccggtcat cagcagaaca
     1381 tcgtgctcaa cgaaaagctt gtcgagcaga gccagcgctt ccgtttcgaa gcgggctgcg
     1441 ttgtaatagt cgtgaatgga gagattgcct ataaaaaaat gtttggtcgc ttctaagtct
     1501 ttgggatcgg gagcagcagt gccaatccgc atttcgcgat acatttggcg ggagtcacaa
     1561 gaaataacgg gagaattcag gaactgtgcc agctttagac tcaggcgggt tttgcctacg
     1621 ccggtgggcc ccgtcaaaac caccagtagt ttcatgttgc aggaaattaa taatcgtcca
     1681 gagagggatt atctccaaag ccttccaggt cgctaaagcc ttcggggtcg agttcttcgg
     1741 ggtcgaagtc gccttctccg taaaagtcgt cgaggctgtc gtcttcgctc acaacagcgg
     1801 tgctgacctt ttcttttttg tcgtgctcaa aacccatgtt ttgtttgggt gcttttcccc
     1861 ttgaaaaaac acattccggt tggctaagcc gtttggtggg aaagatttca accagctcca
     1921 tatagaaagc tctttccgtg agattatcaa acacatagat caggcgttgc ccttcttcgt
     1981 cgagcatctc gctcaggcgg gtggtgccca ttacccagga atcgtattca gggctgcttt
     2041 ccatttcaat cagagtcacc tcaagttctt tttcccattt ttcgtcgcac ataaaaaaag
     2101 aggtgatcat atttttttca tagtccacac tttccaggat ggtattgtgc aagtcaagga
     2161 aataagcctc cgaatcaatc ataatttccc tacaaaagag atcggattca tcagaaagga
     2221 gtcgaaattt gtaaaccata acagaggaat gagtgaaggt tgaacttttt ctgcttaggg
     2281 tagatagcct ttaatgatgc ctctgggtgc attctccacg aagtccagta tgatgtctct
     2341 ttccttactt ggaggcattt cttcacgtat ctgcttcaga gcatcactga cattacgtcc
     2401 cgacatatag atgtggcgat aaacatcttg aatggtgtgg atttgtttat cggtgaaatt
     2461 tcttcggcgc aagcctacag aattgatgcc gcaataaccg agaggatctc tgcctgccat
     2521 ggaaaaagga ggaacgtcct tgcccacctt ggaaccgccc tgcaccatac aatgcatgcc
     2581 aatatgggta aattgatgca cgagcactcc gccgctgata acggcaaagt cgtcgactac
     2641 tacttcgccg gccaggttgg tagcgttgga catgatgacg ttatttccta tcacgcagtc
     2701 gtgagcaacg tggcaataag ccataatcaa acagttattg ccaatcacag ttttgccttt
     2761 ggaggcagtt ccgcggttga gggttacgaa ttcccggatg gtgttgttgt cgccgatttc
     2821 gagaagagtg tcttcgcctt tgaattttag gtcctggggt attcctccca gcacggctcc
     2881 ggggaatatc cggcaatttt tacctatgcg gcttcccgat aaaatagtag catgagaata
     2941 tatatgacat ccttcgccta tcactacgtc cttatctata aagacaaaag gatcaataac
     3001 gacatcggga gctatttgag cttcggggtg aatacaagac agtgtttgtt ggttcatatt
     3061 tttgttggct tggttttatt cgttgtcgac aggaactcgc tttcgtttgg tcagcatggc
     3121 tgtcatttcc gcttcggtaa ccagttcgtt acctacaaaa gcatagcctt tcatttttgc
     3181 cataccgtgg ctgatgggtg gaatatgata aactttaaat accagagtgt cgccggggac
     3241 cacttttttc ctgaagcgaa catactcaat cttgaccagg taggtaatat atttttcggg
     3301 attttttgtt tgattcatca aaaacaagcc tccggtctga gccatggctt cgagctgtaa
     3361 aacacccggc ataacgggtt cgtcgggaaa atgtccctgg aagaaagatt cgttggcagt
     3421 aaggttttta ataccgataa tataggagtc ggttatttca atcactttat caaccatctg
     3481 aaagggccag cgatgaggca gcaaattgcg aatctgctta gtatccatca gagcgggttt
     3541 tctgtggtcg ataagggggg cctgcacttc ctgacggatg atatccttgc ggataagcct
     3601 ggccagttga ttgtttattt tatgccccgg acgcatggca atgatatgcc ccttgatggg
     3661 ttttcctatc aaggctatgt ctcccaccaa atcgagcaat ttgtgtctgg ccggttcatt
     3721 gtcgtaaagt acgggtttgt tgttgatgta ccccagttta ttgacaattt tatgggggat
     3781 attcatcaca tcggctatac ggtccaactc ttcctgacta acttcctgat cgtaaattac
     3841 caaagcgtta tccaggtctc cacccttgat caggttgtgt ttgagcagtt gttccacttc
     3901 gcgcacaaaa acaaaagtac ggctggcggc tatgtgctta ccgaaatctt ccatccggtt
     3961 cagacgggcg tattgattgc tcaatatgca agagtcgtag ctgatcagtg tggttatgct
     4021 aaaactatcg taaggcagta taattagaga agcacccgtt tcgggatcgc tgacttctac
     4081 cttgctgtga actatgtaaa agtctttttc tgcttcaagt ttctcaactc ccgatttttc
     4141 tattccctcc acaaaatgga tggcgctgcc gtccaaaatg ggaatttcgg gcccgtttac
     4201 ttcgatgatg caattgtcta taccggctgc ataaagagca gaaacagcgt gttctaccgt
     4261 acttatgcta accttgttgt agaccagaac cgtaccgcgt tcggtgtgtt ctacgaattc
     4321 ggccagagct ctgatttcgg gcttaccttc gagatcgatc cgtctgaaac gtatacctgt
     4381 gttggcttcg gccgggagaa aagtagcggt cagttgtaaa ccggtgtgta atcctttgcc
     4441 cgaaacacta aagggctttt tgaggcttaa ttgtttaggc atcgttattg attttttgtt
     4501 ttagagcctc caattcttcg cgtgtggctt ttaattctga aacaagttcc ggtaattttc
     4561 taaagagggt atatgaacgc agatattctt tacttggtat ggcaggagat cccaggagcg
     4621 gatgctcgtt ggttttaacg gtagaaatga tccctgcctg ggcacagacg atcgtgccat
     4681 cggggatatt gatatgtccg gtaattccca cctgtccgcc aaacatacat ttgctgccga
     4741 tttttgttga accggctatt cccgtttgag ctgcaataac cgtattttcc ccgatttcaa
     4801 cgttgtgtgc aaccataatc aggttgtcga gtttaactcc cttgcggata atggtcgatc
     4861 ccatggttgc tctgtccacc gtggtgttgg cacagatttc gacgtcgtct tcgattacga
     4921 cattgccaat ctgaggaatt ttggtgtaac tgccgtcttt ttgcggggcg aaaccaaatc
     4981 cgtccgcacc gatcacgacg ccggcctgca gaatgcaatt gtcgcctatc acacaatcgt
     5041 cgaagacttt gaccccggga tataaaatgc aattctttcc tatgcgaacc cggcgtccca
     5101 ggtaaacctg ggggtaaatc ttactattgt cgcccacctc aacctgagcg ctgaggcagg
     5161 aaaaatggcc cacatacacc tctttaccca gaactgctgt aggatcaaca aaactgtttt
     5221 tgtcgattcc ctgaaatttt tctttgaact gttccgacca ctgcaacaat ttggccaggg
     5281 cttcgtaggg gttttcgact ctgatgagtg tggccgatac cgacttttcg ggcttaaaat
     5341 ctttattgac aagaacggcg ctggccttcg tagtatagag atacgaggtg tatttttcgt
     5401 ttgagagaaa cgtgagcgca ccctgacttc cttcttcaat tttcgaaaaa gtgtttatca
     5461 gtacatccgg attaccctca acggttccgg ataaaaaaga agctatttgg ctggctgtaa
     5521 aatccatgtt aaaaatttgg cgcaaagata taaaatatta ctaataatcg atagttttta
     5581 atttggcttg ttcaacagat caaacttttc tcaggtagga ataggcatat tttctgactt
     5641 tttttgatag aacttcgagg ttaagaatgt ccgaggcctg ggttatgggg cgtatcctgc
     5701 catcgttgta cagaatatcg atgccgtagt ccgcatcgtc gtaaatatgg ctgcttacct
     5761 tatcgagcga tacaaaatac cttgcatcct cagtactgat attcaattgc agggcaatgc
     5821 gttgcaagtc ttcctgaacg cgggaggccg gcgtttcctg ttcggagatc tcaattttaa
     5881 acaatttacg gttaagaaaa ttccgggata aagtggacaa aaccacgtcc ggatggtttt
     5941 gccagttctt tatggccgac caaatgtcgg tatcgtccaa tgctatgaaa tgatccagag
     6001 cgacgtcgct gatttcgccc ggtttaagtt taccggaaag gaaaaaagac aaggccgggc
     6061 tgcaagtcag atcctgacct gtctgcttga gtactttggc tctgtgaagc aaattgtgca
     6121 acattttttc cgctgccaac gaagttttgt gcaggtaaac ctgccaatac atcaaacgcc
     6181 tggccatcag gaaattttcg atggaataaa tgcctttggc ttctaccacc aacctatctt
     6241 cacacacggc cagcattttt atgatgcgcg aagagccgat attgccctcg actactccgg
     6301 taaaaaaact gtcccgcctt aaataatcca agcggtctac atccaattga ctggaaataa
     6361 gctgatgtaa aaattttttg ggatagctgc ctttgaagat ggcgatggcc atatcgagtt
     6421 tcccgttgaa ctctttgttc atacgctcca tcagcaagag agaaattttt tcgtgcgaaa
     6481 gcccgttgaa caaaaccggt tccagtacat gggaaaaagg cccgtgcccg atgtcgtgca
     6541 acataatggc agccacggtg ccttctgctt cggcctcggt gatctcattg cctttgaggc
     6601 gtaattggcg tatggcttcg cttgccaaat gcagggctcc ggtgatgtgt tgaaaacggc
     6661 aatgcctggc tcccgggtat accaggaacg acaagcccag ttgttgtatc ctgctcaaac
     6721 ggtagaggta aggatgctgg tacacctggt agaggaaacc tgtaggtaga ttgatgaacc
     6781 cgaaaacagg atcgtttatt attttttgtt tcatccccat aaagccttca ggtaagtttg
     6841 catttcttgc tgaagcttca aagcttcctg gcgggctgcc tgagcaaatt cttcgtcttt
     6901 tgaagcataa agtatttgtc gagaggcgtt tactaacaag ccgcagtcgt cgttcatcgc
     6961 atagcgtgcc acttccttca ggctgcctcc ctgggctccc acgcccggaa ccaacaagaa
     7021 atgacgagga accagttttc ttacatcctg aagcagactg ccttgagtgg cgccaaccac
     7081 gtacatcatc gaaaattcgt cgccccagtc ggaagagcgg cgaagcactt tttcaaacag
     7141 acgttccccc tgtgcgtctt ccagcatctg gaagtcggcc gaaccccggt tggaagtcag
     7201 ggccagcagg attgcccact tgccgggata ggacaaaaaa ggctgaacac tgtccttacc
     7261 catataagga gccagggtga cggcgtctat gggcaggctt tcgtaaaagc tgcgggcgta
     7321 catttcggca gtattcccta tatctcctct tttggcatcg gcaattatga attgcgtggg
     7381 atctttttcc cgaatgtaac ggaccgtttt ttcgaaagct ttccaaccct caagaccaca
     7441 gcttt
//
LOCUS       DAOSGL010000076         7355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8745_length_7355_cov_4.361233, whole genome shotgun sequence.
ACCESSION   DAOSGL010000076 DAOSGL010000000
VERSION     DAOSGL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8745_length_7355_cov_4.361233"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1534
                     /locus_tag="PKY68_03710"
     CDS             56..1534
                     /locus_tag="PKY68_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH24175.1"
                     /translation="MKKIVLAIISLLFYANVSHSALWIKIGLEDKTVSSVACNSQNGN
                     IYAVADNLLYLSENDGLDWTLLNLDVNQPWVHVYRVLVDAKGRIYAGTNNGIYCSDNA
                     DHSFRAINTGLPHPPNVTAICCPDINSLAIGTNAGVFISDKDSIQWTAFNRNLPERTP
                     NQLLCHRERFYLATNSGIYVSPTDSAHWEQCFFNVARDLAAQDAVIYAATHISGSDYD
                     QLGALYQSFNSGSSWERLSPNEGNVGGHAVFCDENRIWLGTDGGGGLYSPDRGAYWET
                     LNLGPWFTALTFAQRGSALLTGTEFYKHIDDSGGLYIQYNVFSSNRQEIPFPEFITCP
                     GAYNNRLYVELSIPANSKVYYSLDGGTPDSSSLLYLEPIKVDSTTLIKVIAIYEDKTT
                     SIVNPGLFKISKETGLKAIPELDHAALPYPNPCSDFINLTVPDVNANISVINLTGQVV
                     YSDQAVQSGNYRLPVHALKPGLYILQLRTAAGKKDWKFIRQP"
     gene            1798..2121
                     /locus_tag="PKY68_03715"
     CDS             1798..2121
                     /locus_tag="PKY68_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24176.1"
                     /translation="MHVYCHQLSQDLFQWRGVERSGIYHETGLLKEWPAEGPQLLWHY
                     DGLGLGFTSVTIANDRIYTTGVSEDKGYVFALELLFLLNGFVWIYLATSLSLKKDLIR
                     DLRNE"
     gene            complement(2157..3416)
                     /locus_tag="PKY68_03720"
     CDS             complement(2157..3416)
                     /locus_tag="PKY68_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOH24177.1"
                     /translation="MFALADCNNFFVSCERVFNPGLNGRPVVVLSNNDGCIVSRSNEA
                     KKLGIKMGVPLFQVRELVQKHEIVVFSSNYVLYGDMSRRVQETLRTFVPAIEIYSIDE
                     AFLDLRGMEHFDLDALAKKISLTCLRHTGIPVSVGVAPTKTLAKVASKLCKQYPKLRG
                     GCYLYKPADIEKVLKTYPIGDVWGIGRQYTKKLRALGVGTAYEFTQLSAPRVQKMMGI
                     TGLRTWKELRAEACIEFTQANPPKQQICVSRSFSKEITDCKELGMQVSMFCAMACEKL
                     RKQNSHCHQALVFILTNPHKEDCPRHFESTLISFQVPTSSTLEINRAILQALQRIFKP
                     FCAYKKAGVVLMEMVPDSAVQGSLFDELDREKHRRLMKAVDKINQKNGYSSVAVATQS
                     LEGIKMNRRYLSPQYTTKWSDIIKVKV"
     gene            complement(3418..3846)
                     /gene="umuD"
                     /locus_tag="PKY68_03725"
     CDS             complement(3418..3846)
                     /gene="umuD"
                     /locus_tag="PKY68_03725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOH24178.1"
                     /translation="MGSISKLTVYSPQEQGLELPLAEAVRAGFPSPAEDFPDQSLNIT
                     AELVKNPASTFYARVSGNSMTGEGIDDGDLLVVDKSVEPYQGCIAICFIDGEFTLKKV
                     EIHSDHVLLVPANKKFTPIKVTEDNTFLIWGIVRYVIKKR"
     gene            4113..4664
                     /locus_tag="PKY68_03730"
     CDS             4113..4664
                     /locus_tag="PKY68_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24179.1"
                     /translation="MRTLFILIACILFFCSCGKVECPAFPEELMTWVDYKKTDTLRYK
                     SFNDTLSHIVINNHKSERYSFNKNLDCVCGASAGFVSDINKNGYNIIGSCYYSDDLID
                     VSYRFQTSEIEDLFEFRYDLSNNQLLDDLTIINYELNGVVLSNVGMLENTNNIIISKV
                     YFTSEKGLIAFVDNNNTEWKLIE"
     gene            5012..5743
                     /locus_tag="PKY68_03735"
     CDS             5012..5743
                     /locus_tag="PKY68_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24180.1"
                     /translation="MRKTLLFIFCAIFTLGLCYADKTWDFKVWSQATLDQLAADAAGP
                     GNWAVASDTRYENNVDFEAGILMANGQEIAETRGLTFGTLNSGKVRIDHGTEPPRLML
                     NGSNLSFDVLGCKAGNVITVTTLTANTEQERGITATNATRTSSPDVSLEVLVNVFNVV
                     ADGTVTFTTNGGGVHIRDVAVVGEMMALNTNTITARIIETAYYDLSGRMAGRDFENLK
                     QGAYIQKTTYDNGAILVKKFLKPTN"
     gene            6071..>7355
                     /locus_tag="PKY68_03740"
     CDS             6071..>7355
                     /locus_tag="PKY68_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24181.1"
                     /translation="MKKKLLIMILSLWSCLSFSQRAWDFTNWSGDTWNDLAEDTLNWT
                     PYFLIEEDLPPLFKGHYNANGLIFDQTKANGNDIVELQDLSFDYLYSDVISEHVFIDL
                     AYAPSRLRLNGSGVVLNIHNCNQYDTLTVVMEYLDWKNSGDAVAVNLIPFTYGEYKET
                     SVESYLVETTGTAGLYLRSEGALNIFSIHSGMPTEADYELLNFINEMKNDDTTWYEPG
                     DTTWYKPGDTTWYEPGDTTWYEPGDTTWYEPGDTTWSQPENTHKKIWNFRIWSQTTLD
                     QLAADAAEQGNWAVASEIRYENIVNFEAGPLMANGHEITETKGLIFGPMSSSKIRIDH
                     RYEPPRLMLNGSNLSINVLNCEAGDTLIVTTITGNSTTERGITANNAKRISGAEVSLD
                     TVTNIFAVETDGSVELTTNGGGILFYDISIVNGNSW"
BASE COUNT         2097 a         1624 c         1571 g         2063 t
ORIGIN      
        1 gcgctaatca attagaaaaa caccgggcaa agagtctgtc ttaatttccg ttcctatgaa
       61 aaaaattgtc ttagcaatta tcagcttgct tttttacgca aacgtgagcc atagcgctct
      121 atggattaaa atcggtttgg aagataaaac cgtttcttcg gtggcctgca acagccaaaa
      181 cggaaacatc tatgcagtgg ccgacaacct attgtacctg agcgaaaacg atggtcttga
      241 ttggactctg ttaaacctgg acgtaaatca accctgggtg cacgtatata gagtactggt
      301 cgacgcaaag gggcgcatct atgcaggaac caacaatgga atctattgct ccgacaatgc
      361 ggaccattcc ttcagggcga tcaatacagg attaccccat ccccctaacg ttacagctat
      421 ctgttgcccg gatatcaaca gcttagctat aggaacaaat gcaggggttt ttatttccga
      481 caaagacagc atacaatgga ccgcttttaa tcgaaattta cctgagagaa cacccaatca
      541 gttactctgc caccgggaac gcttctacct ggccaccaat tcgggtattt acgtcagtcc
      601 aacggactct gctcattggg aacaatgttt tttcaacgtt gccagagact tagccgctca
      661 ggatgccgtt atctacgctg ccacccatat tagcggtagt gattatgatc aattaggggc
      721 tttataccaa tctttcaaca gtggttccag ctgggaaaga ctctctccca atgaaggaaa
      781 cgttggcggg catgctgttt tttgtgacga aaacagaatc tggctgggga cagatggcgg
      841 aggggggttg tacagcccgg acagaggagc ctactgggaa actctgaacc ttggaccctg
      901 gttcaccgct ttgacttttg cccaaagagg ctccgctttg ctgaccggta cagaatttta
      961 taagcatatc gacgacagtg gcggactgta catccagtac aatgtgtttt cgagcaacag
     1021 gcaggaaatt ccttttcctg agttcatcac ctgcccgggt gcttacaaca acaggcttta
     1081 cgttgaattg agcattcccg caaattctaa agtatactac agcctggacg gcggtactcc
     1141 cgacagctcc tcattgctgt atctcgagcc cattaaagtg gattctacca ctcttattaa
     1201 agttattgct atctacgaag acaaaactac cagtatagtg aacccggggc tgttcaaaat
     1261 tagcaaagaa acagggttga aagctattcc cgaattagat cacgccgccc tgccctaccc
     1321 caatccctgc tcagacttta tcaacctgac agtgccggat gttaacgcta atatcagcgt
     1381 aatcaacctg accggacagg tggtctactc tgatcaggcc gtccaaagcg gtaattatcg
     1441 actacccgtt catgcattaa aacccggcct gtatatattg caactaagaa ctgccgccgg
     1501 aaagaaggat tggaagttta tcagacaacc ttagagtcga acagcattct ttcttgtcca
     1561 tccaaccata cacaggcagc gaatgcctct gagatgtcga aacctgcact ccttaaagat
     1621 tgcccaagtc tttttatctt tttacagaac ttattcaggc tttaactctt taggcggttt
     1681 cggcttgttt gagtttctgg ggatttcttc tatttttgtt ggaattcttt atcaattaag
     1741 tttttaagaa atcagcacta tgacaacaaa acaaattttc gttgccgcga tcggcctgtg
     1801 catgtatatt gccaccagct tagccaagac ctttttcaat ggcggggagt agaaagaagt
     1861 ggcatctatc acgaaacagg tttactgaaa gaatggccgg ccgaaggtcc gcaattgctc
     1921 tggcattacg acggcctggg cctcggcttt acttctgtta ccattgccaa tgaccggatt
     1981 tatactaccg gtgtgagcga agacaaggga tacgtgtttg ccttagagct gttgttttta
     2041 ctgaacggtt ttgtttggat ttatctggct acctccctga gcctgaaaaa agacctgatt
     2101 cgcgacctcc gcaacgaata gccaagacgt cggaacgaat agtcaagaca ggacttttac
     2161 acctttactt tgataatgtc gctccatttg gtagtgtact gaggcgacag ataccggcgg
     2221 ttcattttga tcccctccag gctttgggta gccacggcta cgctgctgta cccgtttttt
     2281 tggttgatct tgtccaccgc tttcatcaaa cgccggtgct tttccctgtc caattcgtca
     2341 aacaaggaac cctgtacagc gctgtccggc accatttcca tcaaaacgac gcccgctttt
     2401 ttgtaggcgc agaagggctt aaagatgcgc tgcagggcct gcaggatcgc ccggttgatt
     2461 tcgagggtgc tggaagtggg cacctgaaag ctgatcagcg tgctttcgaa atgccggggg
     2521 cagtcttctt tgtggggatt ggtcaaaata aaaaccaggg cctggtgaca atggctgttt
     2581 tgtttgcgca atttttcgca ggccatggcg caaaacatcg atacctgcat gcccagttct
     2641 ttacaatcgg taatttcttt agagaaactg cgcgaaacac agatttgttg tttgggagga
     2701 ttggcctgcg taaattcaat acaggcttcg gcccggagct ctttccaggt tctgagacct
     2761 gttatcccca tcatcttttg caccctcgga gcagaaagtt gcgtaaactc gtaagccgta
     2821 ccgactccca gagcccggag tttttttgta tactgccggc caatacccca gacatcgcct
     2881 atgggatagg ttttaagcac tttctcgata tcggccggct tatacaaata gcaacctccc
     2941 ctgagtttcg ggtattgctt gcagagtttg gaggcaactt tggccagggt tttggtggga
     3001 gccaccccta ccgaaaccgg aataccggta tgacgcaggc aggttaggga aatttttttg
     3061 gccagggcat ccaggtcgaa atgctccata cctctcaggt cgagaaaggc ttcgtctatg
     3121 gaataaattt cgatggccgg cacaaaagta cgcagggtct cttgcaccct cctggacata
     3181 tcgccgtaaa ggacgtagtt ggacgaaaaa acaactatct catgcttctg taccaactcc
     3241 ctgacctgaa aaaggggaac acccattttt atccccagct ttttggcctc gttactgcgc
     3301 gaaacgatgc aaccgtcgtt gttggacaaa acaaccaccg ggcggccgtt caaaccggga
     3361 ttgaacaccc gctcacaact cacgaaaaag ttgttgcaat cggccagggc aaacatctca
     3421 gcgttttttt atcacatagc ggactatccc ccaaatcaaa aaagtattgt cttcggtgac
     3481 tttgatggga gtaaattttt tattagccgg caccagcaac acgtggtcgg aatgaatttc
     3541 gacctttttc agggtaaatt caccgtcgat aaaacaaatg gctatgcaac cctgataagg
     3601 ctccaccgac ttgtccacca ccagcaggtc gccatcgtca ataccttcgc cggtcatact
     3661 gttgcccgaa actcgggcgt aaaaagtaga ggccggattc ttgaccaatt ccgccgtaat
     3721 attgaggctt tgatcgggaa aatcctcggc cggcgaggga aagcccgccc tgacagcctc
     3781 cgccaaaggc aattcaagcc cctgctcctg aggactatat actgtaagtt tcgaaatgga
     3841 acccatacca cctgtttgat aaacaaaggt agcattattt tcgggcaccc aattagactg
     3901 tggactgttt tgctttttgt ttattcaaag ggtttttgtt tgttgtgttt ttttaacata
     3961 gttgatttgt tttatttaat acttacaggc tcttttgtga atatggtagc ttggtgtata
     4021 ctttaactat ttcccttact actgaatatt tcggagcata ggcacccaat atttcgttaa
     4081 tttaccgcta agattttaaa aattgattga ttatgagaac actctttatt ttgattgcct
     4141 gcatcctctt tttttgttct tgcggcaaag ttgaatgccc tgcatttccc gaagagttaa
     4201 tgacttgggt tgattataaa aaaactgata cattgaggta taaatctttt aatgacacgc
     4261 tttctcatat tgttataaat aatcataaat cggaaagata ttcatttaat aaaaatttag
     4321 attgtgtatg cggtgcaagt gctggcttcg tatcagatat taataagaat ggttataaca
     4381 taattggaag ctgttactac tcagatgacc tcattgatgt atcgtacaga tttcaaacca
     4441 gcgaaatcga ggacttattt gaatttcgtt acgatttgtc gaacaatcaa ctacttgatg
     4501 accttacaat tattaattat gaactcaacg gagtcgttct ttctaatgtt ggtatgcttg
     4561 aaaatacaaa caatataatc attagcaaag tgtattttac cagcgaaaaa ggactgattg
     4621 cttttgttga taataacaat acggaatgga agcttattga gtaacaaccg gaaatgcaaa
     4681 acaactacgg agttgggccg gtttttggac cttcgaaatc acctcagtaa aaatggaaaa
     4741 gaatcaagta ccagtggatt ccttttgatg cctatctcag ttttcaaaac atcaaagaac
     4801 gcttgtcgtt tgtgctcaac tgactaaccg aaaaagacag tcagacacac taattgaaac
     4861 actaccaaaa atcatattac tagaccataa agatttaatc atttttaaca cctctacccg
     4921 cttaaggact tgaaattatt aatttattta tatctttgcg gcaattctta tgctttttta
     4981 ataagttatt gtttaaccta atctacacac catgagaaaa acactactct ttattttttg
     5041 cgcaatcttt acgctgggtc tgtgttatgc agacaaaacc tgggacttca aagtctggag
     5101 ccaggccact ctggaccaac tggcagccga tgctgccgga ccgggaaact gggcagtagc
     5161 ttctgatacc cgttacgaaa acaacgttga ttttgaagcc ggtatactaa tggctaatgg
     5221 tcaggaaatt gctgaaacca gagggctcac atttggaacg ctgaattctg gcaaagtccg
     5281 catagaccat ggcactgaac cacccaggct gatgctgaac ggaagtaatt tatcgtttga
     5341 tgttttgggc tgcaaagccg gcaatgtgat cactgtaaca acacttacag cgaataccga
     5401 acaagaacga ggcatcacgg ccaccaacgc taccagaact tcgtctcccg acgtatcgct
     5461 ggaagtttta gtgaatgtat ttaacgtagt agccgacgga acagtaacct tcaccaccaa
     5521 cggagggggc gttcatatac gcgatgtagc agttgttggt gaaatgatgg cattaaacac
     5581 caacacaatt acggccagaa tcatagaaac agcttattat gacctaagtg gcagaatggc
     5641 cggcagagat ttcgaaaacc tgaaacaagg ggcctatatc cagaaaacca cctacgacaa
     5701 tggtgccatt ttggtaaaga aatttttgaa accaaccaat tgattcgttt cttgatacaa
     5761 gaccctaaaa gtaaaaaatg atttgaatca tactcttttc actaagagat gagtccctcc
     5821 ataaaagcct tccaattaca acagtaaaag gatggttttt tatatctaag agatggttga
     5881 aaatagtagt agaaaaatcc ctgatttttt tttaaaatag tttgttttat aaaatcaata
     5941 aatatatttg catcgaaaag tttgtgttct attttaaaag agaggttttt cttttttgtt
     6001 ttaactactt aacaaccttc ccttttgtct ttattttaaa gcaattatta acccttttat
     6061 ccccctaagt atgaaaaaaa aactacttat tatgattctg tctctctggt cctgcctgag
     6121 cttttcacaa agggcctggg actttacgaa ttggagcgga gatacctgga atgatttagc
     6181 tgaggatact ctgaactgga cgccttattt tttgattgag gaggatttac cacccctttt
     6241 caaaggtcat tacaatgcca acggattgat ctttgatcag accaaagcca atggcaacga
     6301 cattgttgaa ctacaagact tatcgtttga ttatctttac agtgatgtta tttctgaaca
     6361 tgtattcatc gatttagcct atgccccttc cagactgaga ctaaacggaa gcggtgttgt
     6421 actaaatatt cacaactgca atcagtacga cactttaact gtggtgatgg aataccttga
     6481 ctggaaaaac agtggtgatg ctgtagccgt aaatttgatt ccgttcacct atggtgaata
     6541 caaagaaacc agcgttgagt catatctggt tgaaacaacc gggactgccg gactttatct
     6601 tcggagcgaa ggtgccttga acattttcag cattcactcg ggtatgccca cagaagccga
     6661 ttacgaattg ttgaatttca tcaacgaaat gaagaatgat gataccacct ggtatgaacc
     6721 cggtgatact acctggtaca agccaggaga cactacttgg tatgagcctg gagacactac
     6781 ctggtatgag cctggagaca ctacctggta tgagcctgga gacactacct ggagtcaacc
     6841 tgaaaacaca cataaaaaaa tatggaattt tagaatttgg agtcagacta ccctcgacca
     6901 attggcagcc gatgctgccg agcagggtaa ctgggcagtg gcttccgaaa tccgctacga
     6961 aaacatcgtc aattttgaag ccggcccgct gatggccaat ggtcatgaaa tcactgaaac
     7021 aaaaggtctt atctttggcc caatgtcttc aagtaaaata cgaattgacc atagatatga
     7081 acctccgaga cttatgttaa atggaagtaa cttatcaatc aatgtattaa attgtgaagc
     7141 tggagacaca ttaattgtaa ctactattac aggtaatagt acaaccgaaa gaggtatcac
     7201 agccaataat gctaaacgaa tatctggtgc agaagtgtca ttagataccg tcacaaatat
     7261 ctttgcagtt gaaactgatg gaagcgttga gcttactact aacggaggag gtatactttt
     7321 ttatgatata agcattgtca acggaaattc ttggt
//
LOCUS       DAOSGL010000077         7349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8754_length_7349_cov_4.292569, whole genome shotgun sequence.
ACCESSION   DAOSGL010000077 DAOSGL010000000
VERSION     DAOSGL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8754_length_7349_cov_4.292569"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKY68_03745"
     CDS             complement(<1..376)
                     /locus_tag="PKY68_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24182.1"
                     /translation="MKRLILSFFLFIATVGLASAQGLFETAGKSPDNQSEKTVKLGGY
                     VRGSAYGAGENYDFGTLFGETALQLNHKAKLLTMNTDLRFRSGNRFGGSFSEFEIKEA
                     YAGISSKTVDVLLGEQIVSWGRT"
     gene            complement(505..1596)
                     /locus_tag="PKY68_03750"
     CDS             complement(505..1596)
                     /locus_tag="PKY68_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH24183.1"
                     /translation="MDVIVWIGFAQALFAGILTITKDKKSSADYLLSAWLFLLSIEFL
                     TVGIELYVYTNFVFLSNTFLLFNPALFLYTRSLVKPSFRLKWPQLLHLLPYLIFKIFS
                     WSLQEPLSFEGFFSPDNTLWYRISFGVFGLVSWIVYLGLTAVILSRHRKRLKNEFSTI
                     DRIKKMGWIMFVVIFYGIYCLIVLLWGLFNIIVFTTESLTLFNYSVLLFFVYIFSFYG
                     LKQRDIFPDKTKEEPAVPEKNSNSFLSTDQMEKISQKLLNYFQKEKPYLNPEFSMSLL
                     SESLNIPRHHLTEVLNKHLGKNFFLFVNEYRVAAVKKQLDKPDNPYSIEAIGYECGFN
                     SKSTFFSVFKTLTGQTPKNYQQEVLQKMN"
     gene            1763..3142
                     /locus_tag="PKY68_03755"
     CDS             1763..3142
                     /locus_tag="PKY68_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOH24184.1"
                     /translation="MKNYSLVALLLLTVFGIQAQLPVRNAENIEKYKNICRTYIHKEM
                     KGMYREAGGALPYPFLAPGSNQYLDMLWDWDSWLSNVALRQILLEKGKEKDREEALKY
                     EQGCVLNALSYGGMDGWIPIWIERDAPSRQEMLSTRNPWKSNMHKPCLAQHAAFIVRN
                     INGDAEWLRESFYFLQAFVNKYLNFHRHQATGLMYWQTDEAIGVDNDPSTFYRPDASS
                     GSIFLNCLMYKELLAMDYLAQCLNLKDIATFYYKEAARLLENIREHCWDPRDGFYYSV
                     DLNLRPIEKPDIEFLEAGKLYLHTGQPRTYHCLIQRFSVWSGFMAMWSGVATDEQAAL
                     MVERHFHDTTSFNSASGIRSLSPLEKMYDTRGSGNPSSWQGPIWINVNYLVFRGLINY
                     GYKEEARELAEKTILLLGRDIERFGAMHEYYLPDNGEPVLNRGFQNWNFLVLNMAAWL
                     DGKDVVWEF"
     gene            3154..3324
                     /locus_tag="PKY68_03760"
     CDS             3154..3324
                     /locus_tag="PKY68_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24185.1"
                     /translation="MSRFIYIITLLCLLPVTDLRAGNNREKPRVIVTTPSPLPAGGCE
                     GLLSFVVFVSKM"
     gene            complement(3439..3522)
                     /locus_tag="PKY68_03765"
     tRNA            complement(3439..3522)
                     /locus_tag="PKY68_03765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3487..3489),aa:Leu,seq:taa)
     gene            complement(3721..4152)
                     /locus_tag="PKY68_03770"
     CDS             complement(3721..4152)
                     /locus_tag="PKY68_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24186.1"
                     /translation="MKKIISLLLFLGFFACGRYRSSIPDSPVFMRRNLNTIKCLFPGD
                     FYYITEPETVTDRLGYGGLVLLKGFDDRYYAYDLACPVECRPDVKVGQPNEVLEVVCP
                     QCGETYQLGFGLGTPSTGVSKEGLKRYAVVVDVNSEVLVQH"
     gene            4310..6397
                     /locus_tag="PKY68_03775"
     CDS             4310..6397
                     /locus_tag="PKY68_03775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOH24187.1"
                     /translation="MKKSVLILCTLMMISCAKKQGNPFFEDYDTPYGAVPFDKISIEH
                     YMPAFVEGMNRQKLEIDAMAGNPEAPTFENTIAAMDYSGELLNKVSSVFFGLISSHTS
                     DEMQAIAQELSPLLSEHSDNIRLNAPLFKRIESLYNNRDSLNLNTEQQRLLEKYYKNF
                     VRSGAALDESRQERLREINKEEALLTLQYSDNVLKETNDYRLVIDKEEDLSGLPQTVV
                     EAAAEAAAGAGLEGKWVFTLHNPSRLPFLQYADNRELREKILKAYYMRGDNNNEADNK
                     EIVRKLVNLRVEKAQLLGYNTFAEYVLEETMAKNPKNVYDFLDGIWPRALRQAKKEVT
                     ELQKLIDREGGKFKLQAWDWWYYAEKLRKEKYQLDEEELKPYFELQNVRQGLFTVCNK
                     LFGINLTPVNNVPVYHPDVEVFEVTDADGSYLGLFYSDYFPRESKRGGAWMSNFREQY
                     VKNEQNVRPHIYNVGNFTKPSGNTPSLLSLDEVETMFHEFGHTLHGMLSQCSYPSLNG
                     TNVTRDFVELPSQIMENWCTHPEVLKMYARHYKTGEPMPEELIEKIQRAATFNQGFIT
                     TELMSAAYLDMYWHDLSEQKDYDVNQFETDCLSRIGLIPEIVVRYRSTYFNHIFNNNY
                     HAGYYSYLWAEVLDADAFQAFVENGLFDQKTATAFRQNILERGGTDEPMTLYRKFRGA
                     DPDPKALLERRGI"
     gene            6521..>7349
                     /locus_tag="PKY68_03780"
     CDS             6521..>7349
                     /locus_tag="PKY68_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOH24188.1"
                     /translation="MQNKGFVTLFAVLLALVSLYYLSFTAAVRSTEKKAKEYAQGDPV
                     LERNFLDSMAQEKIYLGNTYRECLEKEIGLGLDLKGGMNVVLELSVPDVLRALSGYNT
                     DENFNKALAQASLRMRESQRDYLDLFVEEYKAIDPGARLSAIFSTFEMKGRIDPSSSD
                     EKVIQVLREDIKSAIDNSFNVLRTRIDQFGVVQPNIQRLETGGRILVELPGIKEPERV
                     RKLLQGSANLEFWETYELSELYQALIEANNAVRDMIAATEPQKEQAAEAARVEKAPVE
                     "
BASE COUNT         2131 a         1786 c         1672 g         1760 t
ORIGIN      
        1 cggttcgccc ccagcttaca atctgctctc ccagcaggac atcaactgtt ttagaactga
       61 taccggcgta agcctctttg atttcaaact ccgaaaaaga gccgccaaaa cggttgccgc
      121 tgcgaaaacg cagatcggta ttcatcgtta aaagcttggc tttgtgattg agttgcaagg
      181 ctgtttcgcc aaataaagta ccaaagtcgt agttttcgcc ggcaccgtag gccgaaccgc
      241 gaacgtaacc gcccaatttg acggtttttt cgctctgatt atccggcgat ttacccgcgg
      301 tttcaaacaa accctgtgca gacgcaaggc cgactgtggc aataaataga aagaatgata
      361 aaataagacg tttcataaaa gcgcatttta aataattaat gttgcgccaa aatacagttt
      421 gcactgcttc ctacggtctt attttaaaaa gtcggagaag ttgaagaagt acgattttaa
      481 aaagatggac taaacgaatt tcggttaatt cattttctgc aaaacctcct gttgataatt
      541 tttgggagtt tggccggtaa gggttttgaa aaccgagaaa aaagtagatt tgctgttgaa
      601 gccgcattcg taaccgatgg cttcgatcga gtagggatta tcgggtttat ccagttgttt
      661 tttaacggct gctacccggt attcgttcac aaacagaaaa aagtttttgc ccaggtgttt
      721 gttcagcact tccgtgagat gatgccgcgg tatatttaag ctctccgaca aaagactcat
      781 gctgaattcc gggtttaaat agggtttttc tttttgaaaa tagttcaaca acttctgact
      841 tatcttttcc atttgatcgg tactcaaaaa agaattggag ttcttttcgg gaacggccgg
      901 ttcttctttt gttttatcgg gaaaaatatc gcgctgtttg agcccgtaaa aactgaatat
      961 gtaaacaaaa aacaagagga ccgaataatt gaaaagcgtc agcgattcgg tggtaaatac
     1021 tataatatta aacaaacccc acaacagcac tatcaggcaa taaataccat aaaaaatcac
     1081 cacaaacatg atccagccca tttttttaat gcggtcgatg gtggaaaatt cgtttttaag
     1141 ccttttgcgg tgacgcgata aaatgacagc agtgagcccc aaatagacta tccaggaaac
     1201 caggccgaaa acaccaaacg atatccggta ccagagcgta ttgtccgggc tgaaaaatcc
     1261 ctcaaacgac aaaggttcct gcaggctcca cgaaaatatt ttaaagatca aatagggcaa
     1321 taaatgcagc aattgcggcc atttgagacg aaaggagggc tttaccagag aacgggtata
     1381 gaggaacaaa gccggattga acagcaaaaa agtgttggat aaaaacacaa aattggtgta
     1441 gacatacaac tctatcccga ccgtaagaaa ttctatactc aataagaaaa gccaggccga
     1501 cagcagataa tcggctgaac tttttttgtc tttagttatg gtaagaatcc ctgcaaaaag
     1561 ggcctgggca aaacctatcc agactataac atccatgctc gcaattttcg ccaaaggtac
     1621 agaaattaat ctttgagcgc acagaaaaaa aacctacttt tgggacatct tatttcaaca
     1681 tcccggcttt aaaggcttga aaaaccggtg aaaccaattt gttttcaccg gcctgattca
     1741 ccaagcgtca tttataaaca ttatgaaaaa ttattcttta gtcgcattgc tcctgctcac
     1801 cgtattcggc atacaggccc aactccccgt acgaaacgcc gaaaacatcg aaaaatacaa
     1861 aaacatctgc cgaacctaca ttcacaaaga aatgaaaggt atgtaccgcg aagccggtgg
     1921 agctctgccg tatccctttt tagcgcccgg cagcaaccaa taccttgata tgttgtggga
     1981 ttgggattcc tggctgagca acgtagctct gcggcaaatt ctactggaaa agggcaaaga
     2041 aaaagaccgc gaagaagctc ttaaatacga acaaggctgt gtgctgaacg ccctgagtta
     2101 cggcggtatg gacggatgga ttcccatctg gatagaacgc gatgccccct cgaggcaaga
     2161 gatgctgagc acgcgcaatc cctggaaaag caatatgcac aagccctgcc tggctcaaca
     2221 cgccgccttt attgtgcgca acatcaacgg cgacgccgaa tggctgcgcg aatcgtttta
     2281 tttcctccag gcctttgtaa ataaatacct caattttcac aggcatcagg ctaccgggtt
     2341 gatgtattgg caaaccgacg aggccatagg cgtggacaac gatccttcca ctttttaccg
     2401 ccccgatgca agctccggct ccatctttct gaactgcctg atgtacaagg aactgctggc
     2461 tatggactac ttagctcaat gcctgaactt aaaggatatc gcgacttttt actacaaaga
     2521 agccgcccgg ttgctcgaaa acatccggga gcattgctgg gatccgcggg acgggtttta
     2581 ttacagcgtc gatttgaacc tgcgccccat agaaaaaccc gacattgagt ttttggaagc
     2641 cggcaaactc tacctgcata ccggccaacc ccgcacctat cattgcctga ttcagcgctt
     2701 cagcgtttgg agcggattta tggccatgtg gtcgggagtt gccaccgatg aacaggcagc
     2761 cctgatggtg gaacggcact tccacgatac cacttcgttc aactcggcct ccggtatccg
     2821 gagcttatcg cctctcgaaa aaatgtacga cacccgtggc agcggcaacc cctcttcctg
     2881 gcagggcccg atatggatca acgtcaatta tctggtgttc cgcgggctga tcaactacgg
     2941 ctacaaagag gaagcccggg aattggccga aaaaaccatc ctgttgctgg gcagggacat
     3001 cgaacgcttc ggagccatgc acgaatacta cctgcccgac aacggagagc cggtgctgaa
     3061 ccgtggcttt caaaactgga atttcttagt cttgaacatg gccgcctggc tcgatgggaa
     3121 agacgttgtt tgggagtttt aaaatataac tgtatgagtc gcttcattta cattatcacc
     3181 ttactttgtc ttctgcccgt caccgacctg cgggcaggaa acaacagaga aaaaccgcgc
     3241 gtgatagtca ccacccctag tccgctaccg gcgggtggtt gtgagggttt gttgagtttt
     3301 gtggtttttg tttcaaagat gtagccggag ctagactcaa gagggatacc ttattcataa
     3361 tatgtcgaat tttagagaaa atcatcaaga agaaacacag aagccccacc caatcgatcg
     3421 attttgtggg gcttccggtg cccgaaaccg gactcgaacc ggtacagcct caatggccaa
     3481 aggattttaa gtccttcgtg tctaccattc caccattcgg gctgcctttt acataacagg
     3541 aacgcattaa tggcttttac ataagttgct tggcatgttg aatagcttcg catgcttaaa
     3601 aaaaccgcta atgcggctgc aaagataaag ctttagctcg aaaacggaat attttactga
     3661 tttttttttg acgatctccc ggcaataaac taaaatcacg ctttataaga aggcgtataa
     3721 ttagtgttgt acaagtacct cggaattcac atccaccacc accgcgtatc gtttcaagcc
     3781 ttctttcgac actccggtgg agggtgtgcc caggccaaag cccagttggt aggtttctcc
     3841 gcattgtggg cagaccactt ccagaacctc gttgggttga ccgactttaa cgtcgggacg
     3901 gcattctacc ggacaggcca gatcgtaggc atagtaccgg tcgtcgaaac ctttgagcaa
     3961 caccagacct ccgtaaccca ggcgatcggt tacggtttcg ggttctgtga tataatagaa
     4021 gtctcccggg aataggcatt taatggtatt caggttgcgc ctcataaaaa ccgggctgtc
     4081 gggaatgctc gaccgatagc gtccgcaggc aaaaaagccc aaaaacaaca acaaagagat
     4141 tatttttttc actacagctt tttttaatgc cgacaaatcg tacccttatg cggctgaatt
     4201 tatcaggtct caaagataca tattcctcca gccacttcgg atgttttagc caaaaaataa
     4261 ggtataactc atttaaatcc attatttttg tgctcaaaaa attgaaaaaa tgaaaaaatc
     4321 ggttcttatt ttatgcacac ttatgatgat ctcctgcgcc aaaaagcaag gtaatccttt
     4381 ttttgaagac tacgacaccc cttatggtgc cgtgcctttc gataaaatca gtatagaaca
     4441 ttacatgccg gcttttgtgg aaggcatgaa ccggcaaaag ctcgaaatcg atgccatggc
     4501 cggcaacccc gaagcgccta ctttcgagaa taccattgca gctatggact attcgggcga
     4561 attgctcaat aaagtatctt cggtattttt tggtttaatt tcgagtcaca ccagcgatga
     4621 gatgcaggcc attgctcagg aattgagccc cttgctctcg gagcacagcg acaatattcg
     4681 cctaaatgcc ccgcttttta agcgcatcga aagcctctac aacaatagag acagcctgaa
     4741 tctgaacacc gaacagcagc gtctgctcga gaaatactac aaaaactttg tgcgctccgg
     4801 ggccgctctc gacgaaagcc ggcaggaacg tctgcgcgaa atcaacaaag aagaggctct
     4861 gctcaccttg cagtattccg ataatgtgct caaagaaacc aacgactacc gcctggtgat
     4921 cgacaaggaa gaagatttgt ccggcttgcc gcagacggtg gtggaagccg ccgccgaagc
     4981 tgccgccggg gccggtctgg agggcaaatg ggtctttacc ctgcacaatc ccagccgcct
     5041 gcccttttta caatacgccg acaatcgcga gcttcgtgaa aagatactca aagcttacta
     5101 catgcggggc gacaacaaca acgaagccga caataaggaa atagtccgca aactggtcaa
     5161 cctgcgtgta gaaaaggctc aactgctggg ttacaacact tttgccgaat acgtgctgga
     5221 agaaaccatg gcaaaaaatc cgaaaaatgt gtacgacttt ttggacggca tctggccacg
     5281 agccctgcgg caagcgaaaa aagaagtaac agaactccag aaactcattg accgcgaagg
     5341 cggtaaattc aaactgcagg cctgggattg gtggtattac gccgaaaaac tgcgcaaaga
     5401 aaaatatcag ctcgacgaag aagaactgaa accttatttt gagttgcaaa acgtacgcca
     5461 aggtttgttt acggtgtgca acaaattatt cggcatcaat ctgactccgg taaacaacgt
     5521 gcccgtttat catcccgatg tggaagtttt cgaggtaacc gatgccgacg gctcctacct
     5581 gggtttgttc tactcggatt atttcccccg cgaaagcaaa aggggcggag cctggatgag
     5641 caatttcagg gaacaatacg tcaaaaacga acaaaacgtc aggccgcata tttacaatgt
     5701 gggcaatttc accaaaccca gcggcaacac tccctcgctg ctcagcctcg acgaagtgga
     5761 aaccatgttt cacgaattcg gacataccct gcacggaatg ctgagccaat gcagctaccc
     5821 cagcctgaac ggcactaacg tgacacgcga ctttgtggag ctcccctcgc aaattatgga
     5881 aaactggtgt acgcatcccg aggtgctcaa aatgtatgcc cggcattata aaaccggaga
     5941 acccatgccc gaagagctga tcgaaaaaat acagcgcgcc gccactttca accagggctt
     6001 tattacaacc gaactcatgt cggccgctta cctcgatatg tactggcacg atctgagcga
     6061 acaaaaagac tacgatgtaa atcaatttga gaccgactgc ctcagccgca tcggactgat
     6121 tcccgaaatc gtggtacgct accgcagcac ctatttcaac catatattca acaacaatta
     6181 ccacgccggt tattattcct atctctgggc cgaagtactg gacgcggatg cttttcaggc
     6241 ttttgtcgaa aacggccttt ttgaccagaa aacggccact gctttcagac aaaacattct
     6301 cgagagaggc ggcaccgacg agccgatgac cctttaccgg aaattccggg gagccgatcc
     6361 cgatcccaag gctttgctcg aacgcagagg catttaagtg gcatgaataa aacaaaagac
     6421 ttgaaattaa atgcctttaa gttctttgtg aataaaaaaa agtctatctt tgcgccctaa
     6481 attaaaaatc aaggaataac taataaaata tccaacaaca atgcaaaaca aaggatttgt
     6541 aactcttttt gcagttctgc tcgccctggt cagcctctat tatttgtctt ttaccgcagc
     6601 ggttagaagc accgaaaaga aagccaaaga atacgcacaa ggcgaccctg ttctggaacg
     6661 taattttctg gattcgatgg cccaggaaaa aatttatttg ggcaacacct acagggaatg
     6721 cctcgaaaaa gaaatcggtc tcggtctcga cctcaaagga ggtatgaacg tcgttttgga
     6781 actgtccgtt cccgatgtac tcagggcttt gtcgggctac aataccgacg aaaacttcaa
     6841 caaggcattg gcgcaggcca gcctccgtat gagagaaagc cagcgtgatt acctggatct
     6901 ttttgtcgaa gaatacaagg ccatcgatcc gggagcccgc ctctcggcca ttttcagtac
     6961 gttcgaaatg aaggggcgca tcgacccctc cagttccgac gaaaaagtga tacaggtatt
     7021 gagggaggat attaagagcg ccatcgataa ttcatttaac gtattgcgca cccgtatcga
     7081 ccagttcggg gtggtccagc ccaatattca gcgtctggaa accggcggcc gtattttggt
     7141 tgaattgccc ggtatcaaag aacccgaacg cgttcgtaaa ctgctccagg gttcggccaa
     7201 cctggaattc tgggaaacct atgaattgag cgaattgtat caggctttga tcgaggccaa
     7261 caacgccgtt cgcgacatga ttgcggcaac tgaacctcaa aaagaacaag cggctgaagc
     7321 tgctcgggta gaaaaagccc cggtagaac
//
LOCUS       DAOSGL010000078         7345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8764_length_7345_cov_3.741975, whole genome shotgun sequence.
ACCESSION   DAOSGL010000078 DAOSGL010000000
VERSION     DAOSGL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8764_length_7345_cov_3.741975"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3281)
                     /locus_tag="PKY68_03785"
     CDS             complement(<1..3281)
                     /locus_tag="PKY68_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24189.1"
                     /translation="MLLLSFPGRVESATYYLTASGAGSAQTASNWNTEPDGSGTAAVS
                     FKVSGDIFIIPPDIDGTVSANWIFNHIQNSVMTLEVNGSLSINQGVVMTLYRKNKCYT
                     TMIVRGSLIFKDSGINQLIGTYEGANNGETRFSLLPGATVKTPNTAGLLGTGTQSLRT
                     TNIIIALDNAANYYFNGSTNQTLSGLPTRVNDLVLEGSGTKTLASNLTVNGYLSLRQT
                     AYFANGGYTLSFGPASSLEYKNTGNRTTGTEFPANFGGAGGLIIDPGTGYTVSLSGNK
                     TALTNIFIRSGTFDMQTYTLSRATSGGTLSIEAGTTLRIGGTGSISANYSVPILNCTS
                     NIEYYGSNQIVRGINYGNLLLSGSGVKTMQPGTTSICNDIIVSGTAQLVAANTLNIGR
                     NVSVGASAKLKGGSHTFNVAGTFQVQGTFEYDSSTVNFNGGSPVVVYPGNFYNIIFSG
                     YGTKTAIGSLNVKGDMTINTEFNAGSFSHSLAGNFVNNGSFIAASSTIVLEGTAMQTI
                     SGSSSTTFHHLTINGTQDFILATSSVVSGTLNLTKGLLSLGNNNLRVSAVSGGSALSY
                     VKTNGLGTLRRRISGGGSEVVFPVGNSTYNPIGITNTTPADSDYYFVRVVDEIPSLAN
                     DSTKTVYHKWYITEFTPGGSALTIRVTYDSSSGVGANFNPAFTPVIGYYTGSKWAAAM
                     PATVSSYTFSSAVGAFVTDDMTGSDDYFCVGSGDAFSASRLAIVDMRPATPFANHPNV
                     SIMIEAQNSSGIPVDLSSTTTYDLTAVNTSFSAIPSGSISAGASRDTLSNLTFTTAST
                     TATVQADRTSGENLSPLSSDTFVVVSGTLYRPYTNVATYWSNAIWIYSSDGGASWTTV
                     PIQKTDFSASDAVIIPTGYITKVNTTASFYNLRIEPGAELEIVTSGSLTFKHSGSENG
                     LVVQGTLHNSGGTLTNTLKSHPVSVRGGTYIHNKVGDTIPEIEWISRGSSLSTCRIQK
                     IPAHGFNQTFQQLEFAHTGSLSIDGDITVENRLSLINGIVQTGSYKIVLAAQATVSSL
                     NNARIQGNIRAYVPNAIEPALLFPLGDANVYSPMNINFEGSTFGSGYLDASTSVQAPP
                     FASGL"
     gene            complement(3350..3772)
                     /locus_tag="PKY68_03790"
     CDS             complement(3350..3772)
                     /locus_tag="PKY68_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOH24190.1"
                     /translation="MAKINKVNATWLRNMAFEAELDGHRLVMDAATEFGGRDLGPRPK
                     PFMLLALAGCTGMDVVSLLKKMRVEFEAFSVEVEGELSEERPIRYTRMHVIYSFKGRD
                     LPLEKLEKAVKMSEEQLCGVGAVYRKCMEVTSEIRVSN"
     gene            complement(3888..5660)
                     /locus_tag="PKY68_03795"
     CDS             complement(3888..5660)
                     /locus_tag="PKY68_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOH24191.1"
                     /translation="MSSNFHPFVLPFTIGTLALFSVLILKYNKWIGKFDRQQKKLIRR
                     NLFTGKLIPAVWEMINEGLFHKKVSRYNRRLGYMHRSIALGWFLLISVGFVETVFHFQ
                     GRGHAPWIGVFFRFFVRDNQTLSAWIFAQIMDLLLLYILSGVTMAVFKSIFSKTVGMR
                     KTTRLKLFDTSLRYSLWSIFPLRLLSESATAALADNGGFLTQSLGNLMGERFAGVVEL
                     PLWTLYSIALGLFLCLLPFSRFSHIFTELMLIYFRKMGLREREKPTGFTRFELSACSR
                     CGICIDNCPLDSELNITDIQPVYLIRDIRNRMPHRTATENCLMCNRCESDCPVGIQIS
                     EIRRQERDKKKLDTHDNYGYIEDVQSFNAIGRVIYFGGCMSHLTPGITESMKKIFDTV
                     GQKYWMMDEERTICCGRPLLQQGFFNQAAELRRKNTQLIMESRSKTLVTSCPICYQAF
                     RKEYNLPIKVVHHTEYIATMLRLGKLRLNRSELRLAYHDPCELGRGCGIYDEPREVLS
                     ALGQLLKLEQEYKNSLCCGYNLGNTVLELDQQLKVRDASLRNLTDAHPDFIATACPLC
                     KKAFQHSTTQPIKDVAELVVENLR"
     gene            complement(5670..6002)
                     /locus_tag="PKY68_03800"
     CDS             complement(5670..6002)
                     /locus_tag="PKY68_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOH24192.1"
                     /translation="MADFGFSISKPQSIDTNDMDVGRLQALEAKVPSFKRCINCGACT
                     ATCSAGQFGSFNIRRIHNLYRWDQRKGLEKELQQCMLCGKCTLVCPRGVNLRYLIIRL
                     RKALADNK"
     gene            complement(6005..7144)
                     /locus_tag="PKY68_03805"
     CDS             complement(6005..7144)
                     /locus_tag="PKY68_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH24193.1"
                     /translation="MKEREKPVHGDDNPKPREVKRRLKATKRISRRKRLEFEEVELPL
                     QVAIIGGGVAGMEAASVLAEHRCPVILFEARPELGVNVKNKYKIFPDFSDAGRLVETM
                     TKAMDHPLITVNKDTEIIEIIREPDAYTLLDNKGVPHTVPAVLLASGYEPFDAKRKEE
                     LGFGIYEGVINSLQLEDMLREHKVLNSLGEDPRRIVFLQCVGSRDEKVGNHYCSKICC
                     ITAVKQAIEVKRQLPDTEVYVFYMDLRLWGQGYEELYREAQEQYNVRFVRGRISEAAS
                     TFDGRVQIKSEDTLLGQPLKMVTDLLVLMIGMEASPGTRKLGKTCGLEGPYGFVRSKS
                     PHLEDNLSDKPGFFLAGTCKRPMHIAEAVNDGRAAALEIIKYLRK"
     gene            complement(7155..>7345)
                     /locus_tag="PKY68_03810"
     CDS             complement(7155..>7345)
                     /locus_tag="PKY68_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase subunit B"
                     /protein_id="HOH24194.1"
                     /translation="TYEEMAGLLLGYDPWELGLQLHQVQSERLLDKLGIHYDPNEKYK
                     SVSGRILPNPEKPQYLRV"
BASE COUNT         1877 a         1799 c         1755 g         1914 t
ORIGIN      
        1 cttaaacccg aagcaaaagg tggtgcctgg accgaggtgg aagcatccag ataacccgaa
       61 ccgaaagtgg aaccttcgaa gtttatgttc atcggactgt aaacattagc gtctccgagc
      121 gggaaaagca atgcgggctc tatagcgttg ggaacatagg ccctaatatt accttgaata
      181 cgggcgttgt ttaaactgga gactgttgcc tgcgctgcca gtacaatttt gtaactgccg
      241 gtttgcacta tgccgttgat caggctcagc cgattttcca ctgttatatc gccgtctatg
      301 ctcaggcttc ctgtatgagc aaattccaac tgttgaaaag tctgattgaa tccatgagcg
      361 ggtatttttt ggatgcggca ggtggataag cttgatcccc ggctgatcca ttcgatttca
      421 ggaatggtat cgcccacttt gttgtggata taagtgcctc cccgtaccga aacagggtgt
      481 gatttgaggg tattggtaag agttccgccc gaattgtgca gagtgccctg taccacaagg
      541 ccgttttcgc tgccggaatg tttaaaagtc agggatccgg aagtaacaat ttccaattcg
      601 gcccccggtt caattctaag attgtaaaaa gaggccgtgg tgttgacttt ggttatatat
      661 ccggtaggaa tgataacggc atcgctggcg gaaaaatcgg ttttttgaat cggaacagtg
      721 gtccagcttg ctccgccgtc actactgtaa atccaaatgg cattggacca ataggtcgcc
      781 acgttggtat aaggcctata caaagtgccg ctgaccacta cgaaagtgtc gctacttaac
      841 ggagacaaat tttcgccact tgtgcggtcg gcctgcacag tggctgtggt actggccgtg
      901 gtaaaagtca ggttgctcag cgtgtcgcgc gatgcaccgg cagaaatact gcccgacgga
      961 atagccgaaa acgaagtatt gacagccgtt agatcgtagg tcgtagttga gctgagatct
     1021 acaggaatgc ccgagctgtt ttgagcttcg atcattatgg acacattggg atgattggca
     1081 aagggagtgg ccggcctcat atctacaatg gccagtctcg aagcgctaaa agcatctccg
     1141 gaacccacgc aaaaatagtc gtcgcttccg gtcatatcgt ccgtaacaaa agctccaaca
     1201 gcagaactaa aggtataact gctaacagta gcgggcatag ctgccgccca tttactgccg
     1261 gtataatacc cgattaccgg cgtaaaggcc ggattgaaat ttgcaccgac tcccgacgaa
     1321 gaatcgtaag taacacgaat tgtcaaagca gagcctccgg gtgtaaattc cgtaatatac
     1381 catttgtggt atacggtctt tgtcgaatcg ttggccaacg aaggtatttc gtccacaact
     1441 cgcacgaaat aataatcact atcggccgga gtcgtattgg taataccaat tggattgtaa
     1501 gtggaatttc ccacgggaaa caccacttcg ctcccgccac cgctaattct tcgtctcaat
     1561 gtgcccaggc cattggtttt gacatagctc aatgcgcttc cgcccgaaac ggccgaaacc
     1621 ctgagattgt tgtttcccaa actcagcaaa cccttggtca ggttgagcgt tccgcttacc
     1681 accgacgaag tggccagtat aaaatcctgg gtgccgttga tagtaagatg gtggaatgtt
     1741 gtggaagagc ttccgctaat ggtttgcatc gcggtgcctt ccagtacaat ggtgctgctt
     1801 gccgcaatga aactgccgtt gttaacaaaa ttgccggcca gtgagtggct gaagctgccc
     1861 gcattaaact cggtattaat ggtcatatcg cccttaacgt tcaggcttcc tatggctgtt
     1921 ttggttccgt aaccactaaa aattatgttg taaaaattac cggggtaaac cactacagga
     1981 ctacccccat tgaaattgac cgtagaagaa tcatactcga aagtgccctg cacctgaaaa
     2041 gtgcccgcca cgttgaaagt atggctgccg cctttcagtt tggccgaggc accgacggag
     2101 acattacgcc caatgttcag agtgttggct gcaacaagtt gggctgttcc cgacacgatg
     2161 atatcgttgc aaatggaggt ggttccgggc tgcatggttt taactccgct acccgacagg
     2221 agcaaatttc cgtagtttat tcctctgact atctggttgc tgccgtagta ttcaatgttg
     2281 ctggtacaat tgaggatggg aacagaataa ttggcagaaa tcgaacccgt tccgccaatt
     2341 cgtaaggtag ttccggcttc aatagacaag gtacccccac tggtagcgcg actcagtgtg
     2401 taggtttgca tatcaaaagt acccgatcga ataaagatgt ttgtgagagc tgttttattg
     2461 cccgacagac tgacggtata cccggtgccc ggatcaataa tcaatccgcc ggctcctcca
     2521 aaattggcgg gaaattccgt tccggtagtt ctgttgccgg tatttttgta ttccaacgaa
     2581 gaagccggtc caaaacttag ggtatagcct ccgttggcaa aatacgccgt ttgtcttaac
     2641 gataagtagc cgttgacggt aaggttggaa gcaagcgttt tggtaccgct gccctccaat
     2701 accagatcgt ttactctcgt gggcaaaccg cttaacgttt ggttggtact gccgttaaaa
     2761 taataattag ccgcattgtc cagcgctata atgatgttgg ttgtacgtag ggattgtgtg
     2821 cctgttccca gcagtccggc tgtattcggt gttttgacgg tagcacccgg cagaagcgaa
     2881 aaccttgttt ctccattgtt ggcaccttca taagttccga tcagttgatt gataccgctg
     2941 tctttaaata tcagactgcc gcgaactatc atggtggtat agcatttgtt ctttctgtaa
     3001 agcgtcataa caaccccctg attgatgctg agcgacccat tgacctctaa ggtcatcact
     3061 gaattctgaa tgtgattgaa aatccagttt gcgcttacag taccgtcaat atccggaggg
     3121 ataatgaata tatccccgga aactttgaag ctgacggccg ccgttccact accgtcgggt
     3181 tcggtattcc aattggatgc tgtctgcgca ctgcctgcac cgctggctgt caaataatag
     3241 gtcgcggact caactctgcc cggaaaggac agcagtaaca acgcgacaat tattattttc
     3301 aagaccgggg acttcatatt gctcaaactc cttcctgtct ataaattcct caattcgata
     3361 cacgaatctc agaagtaact tccatgcatt tacggtacac tgcccctact ccgcacaact
     3421 gttcttcgga cattttcacg gccttttcca gtttttccag gggcaagtcg cgtcccttga
     3481 aactataaat aacgtgcatt ctggtatagc ggataggtct ctcctcgctt agttcaccct
     3541 ctacctcgac actaaaagct tcgaattcca cacgcatctt tttgagtaaa cttaccacat
     3601 ccatacccgt gcagccggcc aatgccagca gcataaaagg tttgggcctg gggcctaaat
     3661 cccggccgcc aaactcggta gccgcgtcca ttacgagacg gtgcccgtcg agttctgcct
     3721 cgaaagccat attccggagc caggttgcat tgaccttatt tattttggcc atatactttt
     3781 ggttttttta tcaattcctt atttcacaag ccggaacgaa catatgttcg ataacaccac
     3841 ggcaaatata gtcttttttt atacaggaag cgatcggagc acgtattcta cctaagattt
     3901 tccaccacca gctcggccac gtccttgatt ggctgcgtag tggaatgttg aaaagctttt
     3961 ttgcacaaag ggcaggccgt agcaataaag tcgggatgag cgtctgtaag attacggagc
     4021 gaagcatccc tcacctttaa ttgttggtcg agttccaaca cggtattgcc caggttataa
     4081 ccgcaacaga gactgttttt gtattcttgt tccagcttca acagttgccc cagggccgaa
     4141 agcacttcgc ggggttcgtc gtaaataccg cagcccctgc ccaactcgca gggatcgtgg
     4201 taagccagcc tcagttcgct gcggttgagc ctgagtttac ccaggcgcag catggttgca
     4261 atgtattcgg tgtggtgtac taccttgatg ggcagattat attctttgcg gaaagcttgg
     4321 taacaaatgg ggcaggaagt caccagggtt ttggagcgcg actccataat gagttgagtg
     4381 tttttgcgcc tcagttcggc tgcctgattg aaaaagccct gctggagcag aggacgtccg
     4441 cagcatatgg tcctttcttc gtccatcatc cagtatttct gtcctacggt gtcgaaaatc
     4501 tttttcatcg attcggtaat gcccggcgtg agatgcgaca tgcagccccc gaaataaata
     4561 acccggccaa tggcattgaa ggactgtaca tcttctatat aaccgtaatt atcgtgggtg
     4621 tctagctttt ttttgtctct ttcctggcgg cgaatttcgg aaatctgtat gcctaccgga
     4681 caatcgcttt cgcaacggtt gcacatcagg caattttcgg tggcggtgcg atgaggcatg
     4741 cgattgcgta tatcgcgaat taaataaacc ggctgaatat cggtgatgtt gagctcggag
     4801 tcgaggggac aattgtcgat gcaaataccg cagcgcgaac aggccgaaag ttcaaatcga
     4861 gtaaaaccgg tgggcttttc ccgttcacgt aaacccattt tgcggaaata aatcagcatc
     4921 agttcggtaa aaatatggga aaaacgagaa aaaggcagga gacagagaaa caaacccagg
     4981 gctatagaat acaaggtcca taaaggcaat tccaccaccc cggcaaaacg ttctcccatt
     5041 aagttgccca agctttgagt taaaaaacct ccgttatcgg ccaaagccgc agtggcactt
     5101 tcggaaagca ggcgcaaagg aaaaatagac caaagcgaat aacgcaaact ggtatcaaac
     5161 aacttcagac gcgtagtttt gcgcatgcct acggtttttg aaaaaatcga tttaaacacc
     5221 gccattgtca ctcccgacaa tatatagagc agcaacaaat ccatgatctg agcaaaaatc
     5281 caggccgaaa gagtttgatt atcgcgtacg aagaaacgaa agaaaacgcc gatccaggga
     5341 gcatgtcccc ggccctgaaa atgaaatacg gtttcgacaa aaccaacgct tataagcaaa
     5401 aaccagccca aggcaatgct acggtgcata taacccaaac ggcggttgta tctgcttact
     5461 tttttgtgaa acaaaccttc gttgatcatt tcccaaactg ccggaataag cttaccggta
     5521 aataggttgc ggcgtatgag ttttttttgc tgacggtcga acttaccgat ccacttattg
     5581 tacttaagaa tcagaacgct gaacaaggcc agcgtaccga tggtgaaagg aagtacaaaa
     5641 ggatgaaagt tggaagacat gggttcagct tatttattgt cggccagggc tttgcgtaaa
     5701 cgaataataa ggtaacgcag attcactccg cgggggcaga ccagggtaca cttgccgcat
     5761 agcatgcatt gctgcaactc tttttccaat cctttgcgct gatcccaacg ataaagatta
     5821 tgaatgcgac gtatgttgaa gctaccaaac tgaccggcag aacaagttgc cgtgcaggct
     5881 ccgcagttaa tgcagcgttt gaaggatgga actttggctt ccaaagcttg caagcgaccc
     5941 acatccatat cgttggtgtc gatcgactga ggttttgaaa tactgaatcc aaagtcggcc
     6001 atagttattt tctcaggtac ttaataattt ccaaagccgc tgcacggcca tcgttcacgg
     6061 cctcagcgat atgcatggga cgtttgcagg tgccggccag aaaaaaaccg ggcttatcgc
     6121 tgagattatc ttccagatgc ggactcttgc ttcgaacaaa accgtaggga ccttcgagtc
     6181 cgcaggtttt gcctaatttc cgggtacccg gcgaggcttc catgcctatc atcaacacca
     6241 gcaagtcggt aaccatcttg agaggttgcc ccagtagagt atcttcggac tttatctgta
     6301 cgcgaccgtc gaaagtggag gcggcttccg aaatgcgacc ccggacaaaa cgcacattgt
     6361 attgttcctg tgcctcgcgg tacaactcct cgtaaccctg accccaaagt ctcaggtcca
     6421 tataaaagac atagacttcc gtatcgggaa gctgacgttt tacttctata gcctgcttta
     6481 ccgcagtaat gcaacaaatc ttggaacaat agtgattgcc cactttttcg tcgcgcgaac
     6541 ctacacattg caaaaagact atgcgcctgg ggtcttcgcc cagactattc aagactttgt
     6601 gttctcggag catatcttcg agctgcaatg aattgataac gccttcgtaa ataccaaaac
     6661 ccagttcctc cttgcgtttg gcatcgaaag gctcgtaacc gctggccagt aaaacagccg
     6721 gtaccgtatg gggcacaccc ttgttgtcga gcaaagtata agcatcgggt tcccggatga
     6781 tctctatgat ctcggtatct ttgtttacag ttatcagggg atgatccatc gctttggtca
     6841 tggtctctac caaccggccg gcgtccgaaa aatcgggaaa aatcttgtat ttgtttttca
     6901 cgttgacgcc cagttccgga cgggcctcaa acaaaatgac cggacaacga tgttctgcca
     6961 gcaccgaagc ggcttccatg cccgccactc cccctccgat gatggccacc tgcaggggta
     7021 attccacctc ttcaaactcg agacgttttc tgcgacttat ccttttagtg gctttcagtc
     7081 tgcgttttac ttctctgggt ttggggttgt cgtctccgtg gaccggtttt tctctttctt
     7141 tcatctccaa agtattaaac acgtagatac tgaggctttt cgggattagg taaaatcctg
     7201 ccgcttacac ttttgtactt ttcgttggga tcgtaatgaa tgcccagctt gtcgagcaag
     7261 cgttccgact gcacctgatg caattgcagt cccaattccc aggggtcgta gcccagtaaa
     7321 agcccggcca tttcttcgta agtga
//
LOCUS       DAOSGL010000079         7290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8866_length_7290_cov_4.304907, whole genome shotgun sequence.
ACCESSION   DAOSGL010000079 DAOSGL010000000
VERSION     DAOSGL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8866_length_7290_cov_4.304907"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKY68_03815"
     CDS             <1..393
                     /locus_tag="PKY68_03815"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24195.1"
                     /translation="RYKQTVCLIIVQADGLIISTSTGSTAYALSVGGPILFPENPSIV
                     LAPVAPHSLTVRTIVLSDRSVIDLRVESRSQNFLVALDGRSFPLPGNAQLRVVRADFP
                     IQIVRRKSQSFVQTLRAKMMWGKDLRNT"
     gene            complement(429..2906)
                     /locus_tag="PKY68_03820"
     CDS             complement(429..2906)
                     /locus_tag="PKY68_03820"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOH24196.1"
                     /translation="MKKNIALPFLCSLLIHLNCFASENLPEQTSAEEKVIQYMTAQLK
                     AFPQEKIYLHHDKPYYSAGDTIWIRAYMVHAALHQPMSYSRYIYVELINDNKQVVSRI
                     KIRPEEGLYYSQIPLKADIAAGKYHLRAYTNYLRNLEEDYFFHKEIYIGNLITPEAET
                     NPGEAYAPMQAAAAKNKEQTSQGNRQQYDIQFFPEGGHLISGNMQTIAFKAIDSEGWG
                     TDVHGRILKNGTDEVCTFNSTHLGMGYFGLYTEPGCRYTAECQNQAGEILNIELPQAT
                     DSCYALSVTQMPDKLHLSILRPYNNPLQEELSIIVHMRGLPLFKSRVSAGTDLLSLPK
                     SALGSGVLHILLLNDRAEVLSERLVFIKGNDRASAEITFDKNVYGRREAAGANLQVWN
                     SIGQGLQGNFSLSITDDNLINTDSNALSIESYLLLCSELKGYIEQPQAYFLPENKAAN
                     SQLDILMMTQGWRRYKIPEVVKGELDLADRYEMEIGSTLCGKIQSYPFRRGLPNINVF
                     SLNSKHGYYDATVTDIGGRFCFDGFEFPDSSIFFVQAEKKPGNVMELVAESEDFPPAL
                     SYGLEPKTALQDETIDDFLASSRDRYFYENGPLVINLEEVQVIGRKEDRAKNLREERG
                     AMYFSSSYTVEAQEIEEMGGATLLDVLIRVPGITLNADNTGVLLRNATPLIQVDNFRG
                     RMEDLSIINLSEVEMIDVLKDPAETALFGNEGQNGVICIYLKRGGRNAPRELGSHQKL
                     ITFMGYTPPVEFYQPKYFISSVKESTKPDLRSTIYWKPNLITDENGQAQIRFYTADLA
                     GTYTLILEGISSEGEILRHVQKIELRK"
     gene            complement(2990..3337)
                     /gene="rplS"
                     /locus_tag="PKY68_03825"
     CDS             complement(2990..3337)
                     /gene="rplS"
                     /locus_tag="PKY68_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOH24197.1"
                     /translation="MDLIKVAEEAFATGKVHPKFKSGDTVTVAYRIREGNKERIQNYR
                     GVVIKINGSGSKKRFTCRKMSENIGVERIFPLESPFIESITVNKVGKVRRAKLYYLRA
                     LTGKKARIKEKRF"
     gene            complement(3472..5379)
                     /locus_tag="PKY68_03830"
     CDS             complement(3472..5379)
                     /locus_tag="PKY68_03830"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOH24198.1"
                     /translation="MEYGFVKVGAALPAVRVGDCMHNAKAIIELMHEAEKEQVAVVCF
                     PELAVTAYTCGDLFFQQHLLDSAEQALGKIAEASSGMKLLVFVGCPLRRNNKLYNTAV
                     CIQDGKILAVVPKTYLPNKHEFYEKRWFSSAGEDTHNKLELLGQQVPFGSNILLCSGQ
                     LKVAAEICEDLWAPIPMSSRHCLAGANLIVNLSAANELSGKNRYLKQLLSQQSARCLS
                     AYVYASCGWGESSTDLVFAGNGYVLENGVILAESKRFHMEKQLICSEIDVECLLSERL
                     ANDYFVEESLSYTKLDFHFVPGKKLKLSRTIHPHPFIPAAGNMSESCEEIFSIQVAGL
                     AQRLHQTHISKLVLGISGGLDSTLALMVAVKAMDKLGLDRKNIHGITMPGFGTSQRTY
                     RNANDLMNSLGVSIKEIDIKAACTQHFKDIGHDPSLHDATYENTQARERTQILMDYAN
                     KIQALVVGTGNLSELALGWATYNGDHMSMYGVNTGIPKTLVSCLLQWLAEHTADPQIK
                     NTLLDIIDTPISPELLPLNSGGKLSQITEEAIGPYELHDFFLFYLIRYGFRPKKILFL
                     ATQAFENRYKPNVIRQWLKVFIERFFKQQYKRSCLPDGPKVGRVNLSPRGGWRMPSDA
                     ECRLWLLDLDD"
     gene            complement(5432..6046)
                     /locus_tag="PKY68_03835"
     CDS             complement(5432..6046)
                     /locus_tag="PKY68_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24199.1"
                     /translation="MRKYRCGCLLGLCLLGIFLFFPQNSGAAGDQKSNRLNETGLFFS
                     SNLKNEPWQQSQNAQAFFAGLEDCRSFTFGVFQNMRLIRGIYYQPELSYVVYNGREDH
                     RLTMISLVPAQIQLGYHLGWVRPYVSGACFGSVVVAARQSDNSPLVMNGLAQRSAWGF
                     YYGGGMDIISAVQLNFKLLHWMEGFNNLRPGPLREYRLGLAILF"
     gene            6231..>7290
                     /locus_tag="PKY68_03840"
     CDS             6231..>7290
                     /locus_tag="PKY68_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOH24200.1"
                     /translation="MNKGWILVLIFWYVGLSSLGSSTRPYLLRDKEKYRAASLWADSL
                     MQDLSPEAKVAQLLMPIVNAGDGAWEKKIEELVQQTGVGGLLFDKGQLEKQVLATNRA
                     QSLAEVPLLISMDAEWGLAMRLEGTPSFPKNTMLGALRDTFVLYDYGLEMARQCRLIG
                     AHINFAPVVDINTNPANPVINTRSFGSDPQRVAAYAAAYAKGLERGGVMAVMKHFPGH
                     GDTSEDSHLELAGLQRSRAGLDSFELVPFKHLIRQGVSGVMTGHLSVPALETDVRLPA
                     SQSAAVIEGLLKKELGFDGLCITDALGMKGASTQSGDICVRALQAGNDILLAPPNPAA
                     DVKAILSAIEQGSLSQQEI"
BASE COUNT         1758 a         1670 c         1874 g         1988 t
ORIGIN      
        1 agatataagc agaccgtctg cctgataatc gttcaggcag acggtctgat tatatctaca
       61 tccaccggct ccacggccta tgccctgagt gtgggcggcc ctatattgtt tccagaaaat
      121 cccagcatcg ttctggctcc ggtggctccc cacagcctga cggttcgtac catagtgctg
      181 agcgaccgtt ccgtaataga cctcagggtt gaaagccgca gtcaaaactt tttagtggcc
      241 ttggacggac gttcatttcc cttgcccgga aacgcccaac tacgtgtggt gagggccgat
      301 ttccccatac agatcgtacg caggaaaagc cagagcttcg tgcaaacgct cagagccaaa
      361 atgatgtggg gcaaggacct caggaacacc tgaattcaac aatcttctct atttgcggag
      421 ttcaatcttt acttgcggag ttcaatcttt tgaacgtgtc gcaagatttc tccctcggag
      481 cttatgcctt cgagaatgag ggtgtaagtt ccggctaaat ctgccgtgta aaagcggatt
      541 tgagcttgtc cgttttcgtc ggtaatcagg ttgggtttcc agtaaatggt gcttctcaga
      601 tcgggtttgg tgctttcttt tacggaagaa ataaagtatt tgggttggta aaactcgaca
      661 ggaggtgtgt agcccataaa agtaatgagt ttttggtggc tgcccaattc ccttggggcg
      721 tttcggcctc cgcgtttaag atagatgcaa atgactccgt tttggccttc gttgccgaaa
      781 agggcggttt cggccggatc tttcaaaaca tcgatcattt ctacttcgga aagattgatt
      841 atgctcaaat cttccattct gcccctgaaa ttgtcgactt gaataagagg ggttgcgttg
      901 cgcaataata cgccggtgtt gtcggcattc aatgtgatgc cgggcacacg gattaaaaca
      961 tcgagcaggg tggcgcctcc catttcttca atttcttgcg cctcgacggt gtagctcgac
     1021 gaaaaataca tagcgcctct ttcttcgcga aggtttttgg ccctgtcttc ttttctgcct
     1081 atcacctgta cttcttcgag gttgataacc agggggccgt tttcataaaa atacctgtcg
     1141 cggctggaag caagaaaatc gtcgatggtt tcgtcctgaa gagctgtttt gggttcgagc
     1201 ccgtaagaga gggccggcgg gaaatcttcg ctttcggcta ccaattccat gacattgccc
     1261 ggttttttct cggcttgtac aaaaaagatc gagctgtcgg gaaattcaaa tccatcaaaa
     1321 caaaaacgtc ctcctatatc cgtcaccgta gcatcgtaat agccgtgttt gctgttcagg
     1381 ctgaaaacgt ttatgttggg caggcctctg cgaaagggat aactctgaat ctttccacac
     1441 aaggtagatc ctatttccat ttcgtagcga tcggccaaat ccagttcgcc tttgacgact
     1501 tccggtatct tgtagcgtct ccaaccctga gtcatcatca agatatcgag ttgagagttc
     1561 gctgctttgt tttccggtaa aaaataggct tggggctgtt caatataacc tttgagctcg
     1621 gaacaaagca ataaataact ttctatactc agggcattgg aatcggtatt aatcaggttg
     1681 tcgtctgtga tgctcaggga aaaattgccc tgaaggcctt gtcctatgct gttccatacc
     1741 tgcaaattgg ctccggcagc ttcgcggcga ccgtaaacgt ttttgtcgaa agtgatttcg
     1801 gcactggccc gatcgttgcc tttaatgaaa accagccttt cgctgagcac ttcagctctg
     1861 tcattgagaa gaagaatatg caacaccccc gagcccaaag cagatttggg aagactgagc
     1921 aaatcggtgc ctgccgagac tctggattta aataggggca ggccgcgcat atggactata
     1981 atggacaatt cttcctgtag tggattgttg tagggtctca ggatgcttag gtgtaattta
     2041 tcgggcattt gggtgacgct tagtgcatag caggagtcgg tggcttgggg gagttcgatg
     2101 ttgagtattt ctcctgcctg attttggcat tctgccgtgt acctgcatcc cggttcggtg
     2161 taaagcccga aatagcccat acccagatgt gtgctgttga atgtgcagac ttcgtctgtt
     2221 ccgtttttta agatacggcc gtgtacatcg gttccccagc cttcggagtc gatggcctta
     2281 aaggctatgg tttgcatgtt gccgctgatc agatggcctc cttcgggaaa aaattgtatg
     2341 tcgtattgtt gccggttccc ctgcgaggtt tgctctttgt tttttgcggc agccgcctgc
     2401 atgggagcat aggcttcgcc cgggtttgtc tctgcttcgg gagtgatcaa attgcctatg
     2461 taaatttctt tatgaaaaaa ataatcctct tcgagattgc gcaagtagtt ggtataggcc
     2521 ctcaggtggt atttcccggc tgcaatatcg gcttttagag gtatctgact gtaatagagc
     2581 ccttcttcgg gtcttatttt gattcttgaa accacctgtt tgttgtcatt gatcagttct
     2641 acgtagatgt aacggctgta gctcatgggt tgatgcagag cggcatgtac catataggcc
     2701 ctgatccaga tggtgtctcc ggccgaataa tagggtttgt cgtgatgcag gtaaatcttt
     2761 tcctggggga aagccttgag ctgggctgtc atgtattgta tgaccttttc ttctgcactc
     2821 gtttgctctg gcaggttttc tgaggcaaag cagttcaggt ggatcaaaag gctgcacagg
     2881 aacggaagag cgatgttttt tttcatggcg gataggggca atgaatgtgt atgcagacaa
     2941 aaaaagcaac cgcttgcgcc ggttgctttt ctgtatgaat tgagcgccct tagaatcttt
     3001 tttctttgat gcgggctttt ttccctgtca gggccctgag gtaatacaat ttagcccttc
     3061 tgactttacc aaccttattt acggtaatag actcaataaa gggagattcg agggggaaaa
     3121 ttctttcaac tccgatgttt tcggacattt tacggcaggt gaaacgcttt ttgctgccgc
     3181 tgccgttgat tttaataacg acaccgcgat agttctgaat acgttcttta ttaccttctc
     3241 taatgcggta ggcaacagtc actgtgtccc cgcttttgaa ttttggatga actttgccgg
     3301 tagcaaaggc ttcttcggca actttgatca agtccatgat aaatactctt ttaagtcatt
     3361 agcaatgacg caacatcatc gagcctcact atagtcgaca gcggttacgc aaaacggctg
     3421 caaaggaagg aaaaaatatt gagaagtgca accgggcaac taaaaaaata ttcaatcgtc
     3481 caaatcgagc agccaaagac gacactcggc atcgctgggc atacgccagc cgccccgggg
     3541 agaaagattt acccggccta cctttggtcc gtcaggcagg caagagcgtt tgtactgttg
     3601 tttaaaaaag cgttcgataa acacttttag ccattgacga ataacgttgg gtttgtagcg
     3661 gttttcgaaa gcctgcgtgg caaggaacaa gatttttttc ggcctgaaac cgtagcggat
     3721 caggtagaaa aggaagaagt cgtggagttc gtagggacca atcgcttctt cggtaatttg
     3781 gctgagcttg cccccggagt tcaggggcag gagttcggga cttatggggg tatcgatgat
     3841 gtcaagcaga gtgtttttga tctgagggtc ggccgtatgc tctgccagcc actgtaaaag
     3901 acagctgact aaggttttgg ggatgccggt attgactccg tacatcgaca tatggtctcc
     3961 gttataggtg gcccagccca aagccagttc cgataaattg cctgtgccaa ctaccagggc
     4021 ctgaatttta ttggcatagt ccatcaggat ttgggtacgt tccctggctt gggtgttttc
     4081 gtaggtggca tcgtggaggg atgggtcgtg tccgatgtct ttgaaatgct gtgtgcaggc
     4141 agctttgata tcaatttctt ttatgctgac acccagagaa ttcattaggt cgtttgcatt
     4201 ccggtaagtg cgttggctgg tgccgaagcc gggcatggtg atgccgtgaa tgttttttct
     4261 gtccaatccc agtttgtcca tggctttcac agccaccatc aatgcgagag tggaatccag
     4321 acctccggag atgcccaaaa caagtttgga aatatgggtc tgatgcaggc gttgggcaag
     4381 gccggccacc tgaatggaaa agatttcttc gcagctttcg ctcatattgc cggccgcagg
     4441 tataaaagga tggggatgga tggttcgcga cagcttcagc tttttgccgg gcacgaaatg
     4501 gaaatccagt ttggtatagg acagagattc ttcaacgaaa taatcgttgg ccagacgctc
     4561 cgagagcagg cattcaacgt cgatctctga gcagatcaat tgtttttcca tgtgaaagcg
     4621 tttgctttcg gccaaaatca ccccgttttc gagtacgtag ccgttgccgg caaaaaccag
     4681 gtcggtactg gactctcccc agccgcagga agcgtaaaca taggccgaaa ggcaacgggc
     4741 cgattgctgt gagagcaatt gtttcagata gcggtttttg ccgctcagtt cgttagcggc
     4801 cgacaaattg actattaggt ttgcccctgc caggcaatga cggctgctca tgggtatggg
     4861 agcccaaagg tcttcacaga tttcggccgc cactttcagt tgacccgaac aaagcagaat
     4921 gttgctgccg aagggcactt gttgtcccag caattccagc ttgttgtgcg tgtcttctcc
     4981 ggcggacgaa aaccaacgtt tttcgtaaaa ctcgtgttta ttgggcagat aagttttggg
     5041 tacgacggcc aatatcttgc catcctgaat acaaaccgcg gtgttgtaga gcttgttgtt
     5101 ccggcgcaaa gggcagccca caaaaaccag gagcttcatg cctgagctgg cttctgctat
     5161 tttgcccagg gcctgttctg cagaatcgag cagatgctgc tgaaaaaaca gatccccgca
     5221 agtataagca gtaacggcca gttccggaaa acaaactaca gcgacctgtt ctttttcggc
     5281 ctcatgcata agctctatga tggctttagc attgtgcatg cagtctccga cccgcaccgc
     5341 gggcagtgct gctcctactt tgacaaatcc gtattccata ttcaaaagcg ttcaatccgg
     5401 tttatccgaa tactgcaaag ttaaacgatt tttaaaaaag gatggcaagt cccagcctat
     5461 attctctcag cggaccgggc cgcaaattat taaacccttc catccaatgc agtaatttaa
     5521 agttgagttg aaccgcactg ataatatcca ttcctcctcc gtaataaaaa ccccaggcag
     5581 agcgctgagc caatccgttc atgaccagtg ggctgttgtc tgactgcctg gctgcgacga
     5641 ccacactgcc aaaacaggct ccggagacgt agggccttac ccagcctaag tgatatccca
     5701 actgtatttg tgcgggcacc agcgaaatca tggtcaggcg gtggtcttca cggccgttgt
     5761 aaaccacata agacaattcc ggttgataat aaatgcctct gatgagtcgc atattttgaa
     5821 agacaccgaa agtaaaactg cggcagtctt ccagtccggc aaaaaaagcc tgggcatttt
     5881 ggctttgttg ccagggctcg tttttcaaat tagaggaaaa aaacaagcct gtttcattca
     5941 agcggttgga tttttggtcg cctgctgcgc cgctattctg gggaaaaaac agaaagatcc
     6001 ccagtaaaca aagccccaat aaacaaccgc aacggtattt tctcatgtaa gattctgtat
     6061 ttgtgcaaaa gtacgtcatt atttctattg agaagggatt aaaataagtt tttttagctg
     6121 taaaaccgga aaaacaagcc tgggagcgtc tgaaaaaacg tatattcgca gcgcaaaaac
     6181 agacaggttt tgtacacttg gggcgaagct gtccaaaata gggagtatgt atgaataaag
     6241 gatggatttt ggtgttgatt ttttggtatg taggcctttc cagcctggga tcgtccacaa
     6301 gaccctattt actcagggac aaagaaaaat accgtgccgc ctctttatgg gccgattccc
     6361 tgatgcaaga cttgagtccc gaagccaaag ttgcccagtt gttgatgcct atagtcaatg
     6421 ccggtgacgg agcctgggag aaaaaaatcg aagaactggt tcaacaaacg ggagtagggg
     6481 ggcttttatt tgataaaggc cagcttgaaa agcaggttct ggcgactaat agggctcagt
     6541 ccctggccga agtacctttg ttgatatcca tggacgctga atggggtctg gctatgcgcc
     6601 tggaaggtac gccttcgttt cctaaaaaca ccatgctggg agctctcagg gatactttcg
     6661 tgctctacga ttacggcttg gaaatggcca gacaatgccg cctgatagga gctcacatta
     6721 attttgcacc ggtggtggac atcaatacca acccggccaa tccggtcatc aatacccggt
     6781 ctttcggctc tgatcctcaa cgtgtggcgg cttatgccgc agcttacgca aaaggactcg
     6841 aaagaggagg agtgatggct gttatgaagc atttccccgg acatggcgac acttcagagg
     6901 attcgcattt ggagctggcc ggacttcaac gttcaagagc aggccttgac agttttgaat
     6961 tggttccttt caaacatttg atccggcaag gtgtttcggg cgtaatgacc ggacatttgt
     7021 cggtgcctgc gctggaaacg gatgtccggc tgccggcctc gcaatcggct gcggttattg
     7081 agggcttgct caaaaaagaa ctgggttttg acggcttgtg tattaccgat gctttgggga
     7141 tgaaaggcgc ttctacacaa agcggggata tttgtgtcag ggcattgcag gccggcaacg
     7201 atattttgtt ggctcccccc aatcccgcgg ccgatgtaaa agctattctg tcggccattg
     7261 aacagggatc cctgagtcag caagagatcg
//
LOCUS       DAOSGL010000080         7255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8927_length_7255_cov_4.569444, whole genome shotgun sequence.
ACCESSION   DAOSGL010000080 DAOSGL010000000
VERSION     DAOSGL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8927_length_7255_cov_4.569444"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKY68_03845"
     CDS             <1..345
                     /locus_tag="PKY68_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HOH24201.1"
                     /translation="TIVGFNKESEYQAARDVAMQIAAMNPVSLDRASVPAKIVEQELE
                     IGREKARQEGKPENILDKIAQGRLNKFYQESTLMEQAFIKDGKISVAQYLKSVDEDLV
                     PVDFKRVTLNQE"
     gene            complement(413..811)
                     /locus_tag="PKY68_03850"
     CDS             complement(413..811)
                     /locus_tag="PKY68_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOH24202.1"
                     /translation="MPAQDWKERLAALTKISKEQSENTETPRFSSASNPKQERQNPAS
                     SPASVKKGQLCIRFEKRNGKPATIISEFKGSQQELMELARQLKSTLGIGGSAKDDEIL
                     LQGDLRDKIAPYLQKLGYSLKGDYKKNGSR"
     gene            958..1599
                     /locus_tag="PKY68_03855"
     CDS             958..1599
                     /locus_tag="PKY68_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOH24203.1"
                     /translation="MNEIKKGSTVPEPTLRRLPWYLAFLKMAAARGEIRISSTQISRA
                     INVSAPQIAKDLSFLEISGKTRIGYDVQSLISVLEEYLGFTKIRKAFLFGVGNLGAAL
                     LHDKGLSQFGLEILAGFDVNPRVVGTTIQGVPVYHIKDYPGIQAKIDASIGILTVPVR
                     EAQKVAEEIIQGGIRAIWNFTPFRIHVPEDIVVQNTSIYAHLAVMYNRMEAKL"
     gene            1606..2217
                     /locus_tag="PKY68_03860"
     CDS             1606..2217
                     /locus_tag="PKY68_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOH24204.1"
                     /translation="MKIVAVGMNYLPHIQELKSEIPEEPVLFIKPDSALCSDKQPFFL
                     PDFSQEIHYEVELVVKISRLGKNIAEKFAHRYYDEITLGLDLTARDLQRNLRARGLPW
                     EISKGFDQSAVVGHFIPKNRFENIQQLHFRLDLNGKTVQQGSTAEMLFGVDRIIAYAS
                     RFFTLKTGDLFFTGTPCGVGPVHKEDHLEAWLEGEKMLHLRVK"
     gene            2271..6092
                     /gene="porU"
                     /locus_tag="PKY68_03865"
     CDS             2271..6092
                     /gene="porU"
                     /locus_tag="PKY68_03865"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOH24205.1"
                     /translation="MKLYKAIWSLILSCIFTASYAHSLDWYYSLSARGGRLWSFEGAV
                     QIEEKGLPLYSETFRPAADILRPELRFPVWEEIKGKDLQAYKSLLDKFPDSLELHINK
                     GIQRKEAVWDLYFYPFLHKNGRYYRLLSFEWAYIREATAGLLNEDGQEDTPHFVSSLQ
                     EQPGAESPGQRYAENSRLSSGKFIKISVENTGIYKLSFDDLRKMGIDPAKVQLYGYGG
                     QLLPEDFTQPYIDDLPELSFYIDKEQGYLLFYGLGPLSWNYEASKGLFTRLNNHYSTK
                     GYYFVGERSEGTKPIDELPVPDREPDSEVRAYTAYKLHERDLYSLGGTGRECYGEDLM
                     SNPDLNLSFHFDDILSGEQASIVVETAARSTSPGSTRLYVDNSFMGSMNYSAVSSSDS
                     YTYARGSSLSKTFLPQGNTIAVRLQYNNNGVIPKNAHLNYVAINARCRLALNSASLAF
                     RDPLSLGAGKVCRLCLESADAFTRVFEITKTGSMKQVAGELKGTEYQFKVTTDSLREF
                     VAVNLKGNFPKPVVEGQVPNQNLHGLPAPDFVIITHPDFIAEARELAQAHRDHDRMSV
                     QVVTTEQVYNEFSSGTPDATAYRRFLKMFYDRATTAEDIPQSLLLFGDGVYDNRLVTA
                     AFSSGLSSPNKILTFQSKESLDGTSSYVSDDYFGFLDDNEGVNLPYDKMDIGIGRFPV
                     RSKVEARIVVDKTLSYINNRDKGSWKNQLCFIGDDGDNNTHVSHADKLAESVKSNNPN
                     FITHKIYVDAYQRETSSAGTTVPDANKRLNELLGSGLLLLNYSGHGSTTTWASEQLFT
                     IGEIKGMQNKRLPLWVTATCDFCRYDDAVTSGGEYVFLNEQGGGIALFTTSRVVYSGP
                     NYTINKSFIDHVFATKNGRRLSLGEVMCLTKQSESLKHDRNKLNFALIGDPALKLSYP
                     EYKLEITHVNGRELQAGVYDTLSALSTAEFGGRVLRYDGSFADDFDGLVYPVVYDAED
                     LVETLGNSGNDVFKYYDRSKVLFAGKEQISKGRFTFRFIVPKDLSYSYETGMINLYAY
                     NTRGDIEAQGVFKQFLLGGTNDAVLPDSTGPVIRLFLNDTSFVDGGKVNTKPSLIALL
                     QDESGINMSGMNFGHDLVLTIDGRDRHILNNYYESDLGSGNSGRIIYKLPELETGRHS
                     LSLRAWDLQNNSSVASIDFLVVDDLSPQLRDLNFYQNSEKAWFVFRHDRPASWMQVEF
                     RVYDVWGRLQWRKQVNMMNPDNGSQELEWNYMGENGRRVENGVYICRLFVVSDDNEQA
                     VTAKKIMVGLQ"
     gene            6140..>7255
                     /gene="porV"
                     /locus_tag="PKY68_03870"
     CDS             6140..>7255
                     /gene="porV"
                     /locus_tag="PKY68_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOH24206.1"
                     /translation="MVKYVSPVGAVVTAALLFFSIDLSARDYNPIVTSVPSLSIAPDA
                     HATGLGDVGAATEPNVNSQYWNPSKYAQMENSAGFALSFTPWLSQLVSDINLTNLTGY
                     MKIGDLQSLSASLRYFSLGEVILRQSLGDQGYAIQPYEMALDVAYSRMLSQYYSMSVA
                     LRYIYSDLMSSDDPAGNAFAADISGFYRRPVYFGREQGFWAFGFDVSNIGTKISYDAN
                     NTSYFIPTNLRLGFSVKYPFNSYNAITVSGDVNKLLVPTPQDSTDFATDISSMVGIFK
                     SFTDAPGGLAEELSEINWGIGLEYAYDGQFFVRMGYHFEDKNKGNRKYFTFGAGFKMS
                     MFSIDASYLLSQAQTSPLDQTLRFTLGFDIEGIRNLLG"
BASE COUNT         1865 a         1650 c         1802 g         1938 t
ORIGIN      
        1 accattgtag gtttcaacaa agaatccgaa tatcaggcag cccgcgacgt ggccatgcaa
       61 atagccgcca tgaatccggt ttcgctcgac agagcttccg tgcccgccaa aatcgtagag
      121 caggaacttg aaatcggtcg tgaaaaggcc cgccaggaag gcaaacccga aaatatactc
      181 gataagatcg ctcagggtcg tctgaataaa ttttatcagg aatccacttt aatggaacaa
      241 gcttttatta aagacggtaa gatttcggta gctcagtacc tcaagagcgt agatgaagat
      301 ttagttcccg ttgatttcaa gcgtgttacc ttgaatcagg agtaatcgaa tccggttgta
      361 ttgaatcagg aaatagtatt tccaatccta taaaagaatg gtttccgctt gactaccggg
      421 aaccattctt tttataatcg cctttgagcg aataccctaa tttctgtaag taaggcgcga
      481 ttttgtcgcg cagatcgcct tgtagcaaaa tttcgtcgtc tttggctgag ccgcctatgc
      541 ccaatgtgct tttcaattgc ctggccaatt ccatgagttc ctgttggctg cccttgaatt
      601 cacttataat ggtggcgggt ttgccgttgc gtttttcaaa acggatgcag agctgtcctt
      661 tttttaccga cgcaggacta gaggccgggt tttggcgttc ctgtttcggg ttagaagcag
      721 aactaaacct cggtgtttca gtgttttctg attgttcttt tgagattttg gtcagggctg
      781 cgaggcgctc tttccaatct tgtgcgggca tggagtttga ttgttaattg gttattattt
      841 atatggtcct gccggacttt ggacaaaagt aaactgtctt tatggactca caaaattttg
      901 tgagtccatt agaattattt tattattttt gcgccaaatc tacatttagt attgaggatg
      961 aacgaaataa aaaagggatc aactgtccct gaacctacac tcaggcgtct gccctggtac
     1021 ctggcctttc ttaaaatggc ggcggcccgg ggtgaaatcc gaatatcgtc tactcagata
     1081 tcgagagcca tcaatgtgag tgctccccag attgccaaag atctttcttt tctcgaaata
     1141 agcggcaaga cccgaatagg ttacgatgta cagtcgttga tttccgtgct ggaggaatac
     1201 ctgggtttta ccaaaatacg taaagctttt ttgtttggtg tgggtaacct gggggctgca
     1261 ctgctacacg acaaaggtct gtcgcagttc gggctggaaa ttctggccgg cttcgatgtc
     1321 aatccccggg tggttggtac taccattcaa ggtgttccgg tttatcatat caaagattac
     1381 cccggcattc aagctaagat cgatgcttct atcggcattt tgaccgtccc cgtgcgggaa
     1441 gctcagaaag tagcagaaga aataattcaa ggcggtattc gggccatctg gaattttact
     1501 cctttcagga ttcacgtccc cgaagatatt gttgtacaaa atacttccat ttatgcccat
     1561 ttggcggtga tgtacaaccg gatggaagcc aagctctgag aagatatgaa gattgttgct
     1621 gtcgggatga attatctccc tcatattcaa gaattaaagt cggaaatacc cgaagagccg
     1681 gttcttttta tcaaacccga ttcggctctc tgttccgata aacagccttt tttcctgccc
     1741 gatttctcgc aggaaattca ttacgaagtt gaactggtgg taaaaataag caggttaggc
     1801 aaaaacatag ccgaaaaatt tgcccatcgc tattacgacg aaatcactct gggcctggat
     1861 ttgacagccc gcgatttgca aagaaacctc agagctcgtg gcctgccctg ggaaataagc
     1921 aaaggatttg accagtcggc tgtagtaggg cattttatcc ccaagaaccg gtttgaaaat
     1981 atacagcaat tacatttcag gctcgacctg aacggaaaaa cggtgcaaca gggctctaca
     2041 gccgaaatgt tgtttggggt ggaccggata atagcctacg ccagccgttt ttttaccctg
     2101 aaaaccggcg atctgttttt tacgggtact ccttgtggag tggggccggt acataaagaa
     2161 gatcatcttg aggcctggct cgaaggtgaa aagatgttgc atttaagggt aaaatgacaa
     2221 tagagtggcc gaatttccgt ttcctctctg ttggatagca tcgaaactac atgaaactat
     2281 ataaggcgat ttggagctta atcctgagtt gtatttttac ggcttcttat gcacacagcc
     2341 tcgattggta ttatagcctt tctgcccggg gcggaaggtt gtggtctttc gagggagctg
     2401 tgcaaatcga agagaaaggc ttgccgcttt attccgaaac ttttcggccc gcagccgata
     2461 tactaaggcc ggaactcaga tttccggtat gggaagaaat caaaggcaaa gatttacagg
     2521 cttataagtc gcttttggat aagtttcccg acagtctcga acttcacatc aacaaaggca
     2581 ttcagcgtaa agaggctgtc tgggatttat acttttatcc cttcctgcac aagaacggac
     2641 gttattacag gttgctttcc tttgaatggg cctatatcag ggaggcaact gccggtttgc
     2701 tgaatgagga tgggcaagag gatactccgc atttcgtttc ttcgttgcag gagcaaccgg
     2761 gcgcagaatc gccgggacag cgctatgccg aaaactccag gttgagcagc ggaaaattca
     2821 tcaaaatcag tgtcgaaaat acaggcatct ataagctcag tttcgatgac ttgcgcaaaa
     2881 tgggtataga tccggccaaa gtgcagcttt acggttacgg gggacagttg ttgccggaag
     2941 attttactca accctatatc gacgatttgc ccgaactttc gttttacata gacaaggaac
     3001 aaggctactt gttgttttac ggcctggggc ctttgtcgtg gaattacgag gcttcaaaag
     3061 ggctgtttac ccgtttgaac aaccattatt ccaccaaagg ttattatttt gtaggtgaac
     3121 gctctgaagg caccaagccc atagatgagc tgcccgtgcc ggacagggaa cctgactccg
     3181 aagtcagggc gtacaccgct tataaactgc acgaaagaga tctttacagt ctcggcggca
     3241 ccggcaggga atgttacggc gaagatttga tgagtaatcc cgacctgaac ctgagcttcc
     3301 attttgacga catcttgagc ggagagcagg caagcatcgt agttgaaacg gctgcccgca
     3361 gtacctcgcc gggatctact cgtctctacg tcgacaacag ctttatggga tcgatgaatt
     3421 attcggccgt ttcttcctcc gattcttata cttatgcccg aggcagcagt ttgagcaaga
     3481 cttttctgcc tcagggcaat accatcgcag tgagactgca atacaacaac aatggggtaa
     3541 tacctaaaaa cgctcatctc aattacgtgg cgattaatgc gcgttgccgc ttggcgttaa
     3601 actcggcctc tctggctttc agagatcctc tgagcctggg tgccggcaaa gtctgccgtt
     3661 tatgccttga gtctgccgat gcctttaccc gtgttttcga aataactaaa acgggcagta
     3721 tgaagcaagt ggccggagaa cttaaaggaa cggaatatca attcaaggtt acgaccgaca
     3781 gtttgcggga atttgtagcc gtgaacctca aaggtaattt tcccaaaccg gttgtagagg
     3841 ggcaggttcc taatcagaat ttgcacggtt tgccggctcc ggattttgta atcattactc
     3901 atcccgattt tattgccgaa gcccgggagt tggctcaggc acaccgcgat cacgaccgga
     3961 tgagtgtaca agtggttacc acggaacagg tttacaacga attttcatcg ggaactcccg
     4021 acgctacggc ttatcgccgc tttttgaaaa tgttttacga ccgggcaaca actgcagagg
     4081 atattcctca atctctttta ttgttcggag acggagtata cgataatcgc ctggtgacgg
     4141 ccgccttttc ctcggggctt tcgagtccca acaagatcct gactttccag tccaaagaga
     4201 gtctcgatgg aacatcttct tatgtgtctg acgattattt cggctttctg gacgacaacg
     4261 aaggagtgaa tcttccgtac gataaaatgg atatcggtat agggcgtttc cctgtacgca
     4321 gcaaggttga ggccaggatt gtcgtggaca aaaccttatc ttacatcaac aacagagata
     4381 aaggcagctg gaaaaatcaa ctttgcttta tcggcgacga cggagacaac aatacccatg
     4441 tgtctcacgc cgacaagctg gccgaatctg tcaagagcaa taatccgaat tttataaccc
     4501 acaagattta tgtggatgct tatcagcggg aaacaagttc tgccggtacc acggtgcccg
     4561 acgccaacaa acgactgaac gaattgctgg gttccggatt gctgctgctc aactacagcg
     4621 gtcatggttc taccaccacc tgggccagtg aacaattgtt taccataggc gagatcaaag
     4681 gaatgcaaaa caagcgtttg cctctttggg taacggccac ctgcgatttt tgccgttacg
     4741 acgatgccgt tacctcgggc ggggaatatg tgtttctcaa cgaacaggga ggaggtattg
     4801 ctttgtttac tacttcaagg gtggtgtatt ccggaccgaa ttataccatc aacaaaagtt
     4861 ttatcgacca tgtatttgcc accaaaaatg gccggcgcct gagcctgggc gaagtaatgt
     4921 gcctgactaa gcagtcggag tcgctcaaac acgatagaaa taagctgaat tttgccctga
     4981 taggcgatcc cgcccttaag ttgagttatc ccgaatacaa gttggaaata acccatgtca
     5041 atggcagaga attgcaggca ggagtgtacg atacgctcag cgcactgagt acagccgaat
     5101 tcggcgggcg tgtgctgcgt tacgacggaa gttttgccga cgatttcgac gggcttgttt
     5161 atcccgtcgt ttacgatgcc gaagacttgg tcgagaccct gggcaacagc ggcaacgatg
     5221 tttttaaata ttacgaccgc agtaaggtgt tgtttgccgg caaagagcaa atcagtaaag
     5281 gtagatttac tttccgcttt atagtgccca aagacctgag ttattcctac gaaacgggta
     5341 tgatcaattt gtacgcgtac aatacccggg gagacatcga agctcagggc gtttttaaac
     5401 aatttctgct tggcggcacc aacgacgcag tcttgcccga cagcacagga cctgtgatca
     5461 gattatttct gaacgacacc agctttgtgg acggcggaaa ggtaaacacc aaacccagcc
     5521 ttattgcttt gttgcaggat gaaagcggca tcaatatgag tggcatgaat ttcggccacg
     5581 acttggtgct aaccatcgac ggcagggatc gtcatatcct gaataattat tacgaatccg
     5641 atctgggctc cggcaactcg gggcgtatca tatacaaatt gcctgagttg gaaacaggcc
     5701 ggcacagcct tagcctgaga gcctgggatt tgcaaaacaa ttcctcggtg gcctccatcg
     5761 actttttggt ggtcgacgat ctgagcccgc aattgcggga cttgaatttt tatcagaaca
     5821 gcgaaaaagc ctggtttgta tttcgtcacg atcgtccggc atcctggatg caggtagagt
     5881 tcagggtcta cgatgtctgg ggcaggctgc aatggcgaaa gcaggtcaat atgatgaatc
     5941 ccgacaacgg cagccaggaa ctggaatgga attatatggg cgaaaatggc cgaagagtcg
     6001 aaaatggggt gtatatttgc aggttattcg tcgttagtga cgacaacgaa caggcggtaa
     6061 ccgccaaaaa aatcatggta ggcctgcaat aaaaaggcgc ttgcagagtt aaagagtaat
     6121 aacttacaaa gtttacaaga tggtaaaata tgtcagcccg gttggggcag ttgtaacggc
     6181 agcattgttg tttttttcaa tcgatcttag tgcacgagat tacaatccca tcgtcacttc
     6241 ggtaccctca ttgagcattg ctcccgacgc acatgccacg ggcctgggtg atgtaggcgc
     6301 cgccaccgaa cccaacgtga attctcaata ctggaatccc tccaagtatg cgcaaatgga
     6361 aaactccgcg ggctttgctc tttcgtttac tccctggctc agtcaattgg tgagcgatat
     6421 taacctgacc aacctgacgg gttatatgaa aattggcgat ctgcagtcgc tgagtgcctc
     6481 gttgcgctat ttttcgctgg gtgaggttat tttaaggcaa tccctgggcg atcagggtta
     6541 tgccatacag ccttacgaga tggctctgga tgtggcttat tcccgtatgc tttcgcagta
     6601 ttattccatg tcggttgcct tgcgctatat ttattccgat ttgatgagta gtgacgatcc
     6661 ggccgggaac gccttcgccg cagatatcag cggtttctat cgcaggcccg tgtatttcgg
     6721 acgtgagcag ggtttctggg ctttcggttt cgacgtatcc aacatcggta ccaaaatatc
     6781 ttacgacgcc aacaacactt cttatttcat tcctaccaat cttcgtttgg gattcagcgt
     6841 gaaatatcca ttcaacagct acaatgccat tacggtgtcc ggcgatgtca acaagctgtt
     6901 ggttcctacg cctcaggact ccaccgattt cgccaccgat atttcgtcga tggtgggtat
     6961 tttcaagtct tttaccgacg cccccggagg cctggccgag gagttgagcg aaatcaactg
     7021 gggtatagga ttggaatacg cttacgacgg acaattcttt gtaagaatgg gttatcattt
     7081 cgaagataaa aacaagggta accgcaagta cttcaccttt ggtgcgggat tcaagatgag
     7141 catgttcagt atcgacgctt cctatttgct ctctcaggct caaaccagcc cgctggacca
     7201 gaccttgcgt tttacgctgg gctttgatat agagggtata agaaatctat taggt
//
LOCUS       DAOSGL010000081         7228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8976_length_7228_cov_3.911055, whole genome shotgun sequence.
ACCESSION   DAOSGL010000081 DAOSGL010000000
VERSION     DAOSGL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8976_length_7228_cov_3.911055"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PKY68_03875"
     CDS             <1..1077
                     /locus_tag="PKY68_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOH24207.1"
                     /translation="LPLLAKYGASAVALTLDEQGIPETVEGRLRIAKRIIARAAAKGI
                     NKNRLIFDALVMAVSSNQHYGQLTLDTLRSLKKMGVLTTIGLSNVSFGLPNRPLLNRS
                     FLLMALTSGLDIPILNPLDEENMQSLKAFNALSGADPACENYISYHSPSPVHEQSDSL
                     YQVVLSGLKDSLPLAMDKILKEKEPQQIIDEDLIPALNEVGQRFEKKHVFLPQLIRSA
                     EVARQAFQQLGELYVQKTDKTQGLVVLATVKGDIHDIGKNIVKVVLESHGYRVLDLGK
                     NVDTEKVLEAYRTHRPLAIGLSALMTTTVPSMSQTVEALKEMSAECPVIVGGAVITES
                     LAREMGADYYAADALTAIRILEEL"
     gene            1074..1727
                     /locus_tag="PKY68_03880"
     CDS             1074..1727
                     /locus_tag="PKY68_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH24208.1"
                     /translation="MIQGIDIHKSFGTLEVLKGLDIEVKPGEVVAVVGPSGAGKTTLL
                     QILGTLDRPNRGSVFIDGTRVSALGNREQASFRNRHIGFVFQFHQLLPEFTALENVSI
                     PALIAGKSKKEAEALAMEMLDHLNLSDRINHKPSELSGGENQRVAVARALVNRPKVVF
                     GDEPSGSLDSKNKAELHRLIFDLRERFGQTFIIVTHDKELARLTDRVIEIKDGVILS"
     gene            1739..3184
                     /locus_tag="PKY68_03885"
     CDS             1739..3184
                     /locus_tag="PKY68_03885"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOH24209.1"
                     /translation="MKKILLVALIPVLLSMLSCRHKQTKDARTENQTSKITVLIEYAD
                     STVKVSEQAFGIRVTRVGMHVVAVSRRKNVEFILRGKAAEGSFKLYSDYSFALRLDGV
                     ELFNPEGSAINIQSPQHALILVSPGSENLLRDGSLYAIPPEGEQMNACLYSEGNLIFY
                     GSGSLLLKGNYKHAIYCRGYLKINDNCDIRISEAKKDGIHTLGDVFIEGGQLNINVAD
                     DGLKSAGNILINKGDIVIRALKKDGSEGLECNRLTVNGGQMSVLAYDDAINTARELII
                     NGGSVFCYSLHNDGIDSNGSIQINGGLVVSSGSQMPEEGIDCDRNAFVITGGTVVACG
                     GGTSVPTVRLSTQPSLIYSGDFEQSIHIRGAGGEDVLTYFPQRNYHRFTLLFSSPALK
                     LGSDYRLYSGGQLSGGTQQHGYYTGSTYRRGVKKADFTTSTMVTMIGEFHMGGPIHRG
                     AFPIDPPEEPPFDPADFPQYPPAENQAPGEL"
     gene            complement(3191..5791)
                     /locus_tag="PKY68_03890"
     CDS             complement(3191..5791)
                     /locus_tag="PKY68_03890"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOH24210.1"
                     /translation="MKSKLPALLLGLLLSCPLSCVFANDLSLWYNKPAANWNEALPLG
                     NGRLGAMVYGDALNETIKLNDNTLYSGEPATVWKVHDITPTYDKVVGLLLEGKYAEAN
                     ELVQYHWLGRLHENYQPLADWHLTNHTQGEISDYWRELDIANSVFRMSYKQNGLNYTR
                     EMFISHPDQVIVMKMTCDKQAEFDFSLHFTSHHRATTVFDIEENVYKVSGQAPGHGQR
                     RGDAQIRGWKAEGRHPELYFADGSRKYEENVLYGDQINGLGTFYEARLQVLAPGSKIV
                     SEDKVMRVTGGNEIVFILSAATSFNGFDKSPSRQGKDAVKLAEEALSKAAQKSYENLK
                     KDHVRDYKQLFDRVSFEMPYKKELASLPTDERIDRFYDDYDNGMITLLYQYGRYLMIS
                     ASRPGGQPMNLQGIWNDKVMPSWNCGYTLNINAEMNYWPAEPGNLPECHEPFFQMIKE
                     MAVSGNKTAREMYGRNGWVAHHNTSIWRETHPNDGTPTSSYWPMASAWLSSHLWEHYL
                     YSGDKEFLQNEAYPIVKGAAEFFTDWLVDNGEGYLVTPVSNSPEHNFIYGEYQKAAIS
                     MGSTMDMALIRELFTRTIEASEILGVDEDFRNKLSLQLEKLLPYRIGARGQLQEWQQD
                     FRDGEPRHRHVSHLYGLHPGNQINLDETPELFQASMRTLELRGDEATGWSMGWKINLW
                     ARLLDGDHANIIIRNLFNPIGFGKSKLSGGGLYANMLDAHPPFQIDGNFGYTAGIMEM
                     IVQSHAGFVHLLPAIPREWSSGKISGIKTRGGFELDIEWENGKISRAVLHSSLGGNCR
                     LRSSELLRIKGAKVKTAEGQNPNPLFKTVSAGGFQDYSQVGATKLPDKKYYTVDFTTE
                     KGKSYVITPQ"
     gene            6038..6199
                     /locus_tag="PKY68_03895"
     CDS             6038..6199
                     /locus_tag="PKY68_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOH24211.1"
                     /translation="MKKYRCTVCNYIYDPAEGDPDSDIAPGTAFEDLPDDWTCPLCGV
                     GKEDFEPED"
     gene            6235..6888
                     /locus_tag="PKY68_03900"
     CDS             6235..6888
                     /locus_tag="PKY68_03900"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOH24212.1"
                     /translation="MNSKFVILLTCIIMSIQSAGAKAPFEKPQLKYAHNALEPSISAE
                     TIEYHYGKHLQAYIDNLNKLAAGTAFAEMSLDDIVLKAEGGILNNGAQSWNHIFYFDS
                     FSPKAKTAPGGKLLEAINRDFGSLEAFKEQFNKAAATLFGSGWAWLSVDKNGKLVITA
                     ESNAGNPMRSGLKPLLTIDVWEHAYYIDHRNRRPDAIAAFWKVLDWSVVEARFENKL"
     gene            6947..>7228
                     /locus_tag="PKY68_03905"
     CDS             6947..>7228
                     /locus_tag="PKY68_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOH24213.1"
                     /translation="MKPPYTITPLILRSVSSISEKLGEVNASHFRRPSSSWRKRNALL
                     KVHASLRLEGNKMSLRQIKAVLEQRQVKADPQDILEAQNALKVYDELSLM"
BASE COUNT         1775 a         1709 c         1857 g         1887 t
ORIGIN      
        1 cttcctctgc tggccaaata cggtgcttcg gccgttgccc tcaccctcga cgagcagggc
       61 atacccgaaa cagtggaagg acgtctgcgt attgccaaac gcatcattgc ccgtgccgca
      121 gccaaaggca taaacaaaaa ccgtctcatc ttcgatgctc tggtaatggc ggtttcgtcc
      181 aatcaacatt atggtcagct tacgttagac accctgcgca gccttaaaaa aatgggagtt
      241 cttaccacca taggcttgtc caacgtgtcc tttggtttac ccaaccgtcc tttgctgaat
      301 cgcagttttt tgctcatggc tttgacttcg ggtctggata ttcccatttt gaatcctttg
      361 gacgaagaga acatgcaaag ccttaaggct ttcaatgcct tatcgggggc agaccctgct
      421 tgcgaaaact atatttccta tcattcccca agtccggttc atgaacaaag cgacagcctt
      481 taccaggtgg tgctcagtgg tttaaaagac tcattgcctt tggctatgga taaaatcctc
      541 aaagaaaaag agccgcaaca gatcatcgac gaggatctga ttccggcctt gaacgaggtt
      601 ggtcagcgtt tcgaaaaaaa acacgtattt ttgcctcagc tcatccgttc ggcagaagtg
      661 gcccgccagg cttttcagca attgggggaa ttgtatgttc aaaagacaga caaaactcag
      721 ggattggtgg tgttggctac ggttaagggc gacattcacg atatcggcaa gaacatcgtc
      781 aaggtggtgt tggagagcca tggttaccgg gtgttggact taggcaagaa cgtagatacg
      841 gaaaaagtgc tggaggccta ccgcactcat cgtccgctgg ccataggact cagtgcttta
      901 atgaccacca cggtgccctc aatgagccag acggtggaag cacttaaaga gatgagcgcc
      961 gaatgtccgg tcattgtagg cggggcagtg attaccgaga gcctggcacg ggagatgggt
     1021 gccgactatt acgcagccga cgcattgacg gctatccgta ttttggaaga actatgatac
     1081 agggaatcga tatacacaag agttttggta ctctggaagt actcaaaggt ctcgatatag
     1141 aagtaaagcc gggcgaagtg gtggcggtgg ttggtcccag tggtgcagga aagactactt
     1201 tgctgcagat actaggcacg ctcgatcgtc ccaaccgggg cagtgtcttt atcgacggca
     1261 cccgggtttc cgcgctgggc aaccgggagc aggcttcgtt tcgaaatcgc cacataggtt
     1321 ttgtgtttca gtttcatcaa cttttaccgg aatttactgc tttggaaaat gtgagcattc
     1381 cggctctgat tgcgggcaaa agtaaaaaag aggccgaagc cctggccatg gaaatgctcg
     1441 atcacctgaa tttgtccgat cgtattaacc acaaaccttc ggaactgtcc gggggagaga
     1501 atcaacgggt ggcggtggcc agagccttag taaacaggcc aaaagtggtt tttggggacg
     1561 agccttcggg tagtctcgac agcaaaaaca aagccgaact gcaccggttg atcttcgact
     1621 tacgcgaacg tttcggacag acctttatca ttgtaaccca cgacaaagaa ttggcccgcc
     1681 tgaccgaccg ggttatcgaa ataaaagacg gcgtcatatt atcttaatcg aaagaatcat
     1741 gaaaaaaatc ctccttgtcg cattaatccc tgttttacta agcatgttgt cctgtcggca
     1801 taagcaaacc aaggatgccc ggaccgagaa tcaaacatcc aagatcactg tgttgatcga
     1861 atatgcagac agtacggtaa aagtttccga gcaggcgttc ggcattcgtg tcaccagggt
     1921 gggtatgcat gtggtagccg tctcccgcag aaaaaacgtg gaattcattc tgaggggtaa
     1981 agccgccgaa ggctcattca aactctacag cgattattct tttgccttgc gtcttgacgg
     2041 ggtggaattg ttcaatcccg aggggtcggc catcaatatc caatctcccc aacatgcttt
     2101 gattttggtg tcgcccggaa gtgaaaacct gctcagagac ggttccctgt acgccattcc
     2161 gcctgaagga gaacaaatga acgcttgttt gtacagcgaa ggaaacttga ttttttacgg
     2221 ttcgggaagt ttgctcttaa aagggaatta caaacacgcg atttattgca ggggttatct
     2281 taaaatcaac gataattgcg atattaggat tagcgaggca aaaaaagacg gtattcacac
     2341 cttgggcgac gtgtttatag agggcggtca gctcaatata aatgtggcag acgacggcct
     2401 gaagtccgca ggcaatatcc tgataaacaa gggagatatc gtcatcagag ctttgaaaaa
     2461 agacggctcc gaaggtctgg aatgcaatcg gctaacggta aacggcggcc aaatgagtgt
     2521 gttggcttac gacgatgcta tcaatacggc cagggaattg attatcaacg gcggttctgt
     2581 gttttgctac agtttacaca acgatgggat agacagcaac ggttccattc agatcaacgg
     2641 aggtctggtg gtctcttcgg gtagccaaat gcctgaagaa gggatagatt gcgaccggaa
     2701 cgcctttgtg attaccggag gtaccgtcgt tgcctgcgga ggcggaacca gtgtgcctac
     2761 ggttcgtctg tccacacaac cttcgctgat ttattccgga gatttcgagc agagtattca
     2821 tatccgtggg gccggcggcg aagatgttct gacctatttt ccgcagagaa actatcaccg
     2881 gtttaccctt ttattcagca gtccggcgct caaactgggt tccgattacc gcctatattc
     2941 cggcggacaa ctttcgggag gaactcaaca gcatggctat tacacgggtt cgacctacag
     3001 acgtggggtg aagaaagccg attttaccac ttcgactatg gtaacgatga taggggaatt
     3061 ccatatggga ggccccatac acaggggagc ctttcctatc gacccgcccg aagaacctcc
     3121 tttcgatcct gcagactttc ctcaatatcc tccggcagag aaccaggctc ccggcgagtt
     3181 gtaaagcggg ttattggggt gtgattacgt agctcttgcc cttttcggtc gtaaaatcta
     3241 ccgtatagta ttttttatcg ggcaacttgg tagctcctac ctggctgtag tcttgaaaac
     3301 ctccggcgct caccgtttta aacaagggat tcgggttttg cccttccgct gtttttactt
     3361 tagctccttt aattctgagc agttcggagc tgcgcaggcg gcaattgcct ccaagacttg
     3421 aatggagtac ggcccggctg atcttgccgt tttcccactc aatatccagt tcgaatcctc
     3481 cccgggtttt gattccgctg atttttcccg aagaccattc ccggggaata gccggcagca
     3541 gatgtacaaa accggcatga ctttgcacta tcatttccat aataccggcc gtgtaaccaa
     3601 agttaccgtc gatctgaaag ggtggatgag catcgagcat attggcatag aggccgcctc
     3661 cgctcagttt tgatttgccg aaaccgatgg gattgaatag gttgcgaatt attatattgg
     3721 catgatcgcc gtcgagcagg cgagcccaca gattgatttt ccagcccata gaccagccgg
     3781 tggcttcgtc gcctcggagc tcgagcgtac gcatgctggc ctgaaacaat tcgggcgttt
     3841 cgtccagatt gatttgattg ccggggtgca gaccgtagag atgcgaaacg tgcctgtggc
     3901 ggggttcgcc gtctcggaaa tcctgttgcc attcctgcaa ctggccccgg gcgccgatgc
     3961 ggtaaggcag taatttttcc agttgcagac tcagtttgtt gcggaaatcc tcgtcaaccc
     4021 caagaatctc ggaggcttcg atggttcggg tgaacaattc cctgatcagg gccatatcca
     4081 tggtgcttcc catgctaata gctgcctttt ggtattcgcc atagatgaaa ttatgttcgg
     4141 gagaattgct cacgggagtt accagatagc cctcgccatt gtccaccagc cagtcggtaa
     4201 aaaactcagc cgctcctttg acgatgggat aggcctcgtt ttggaggaat tccttgtcgc
     4261 cgctatacag ataatgttcc caaagatggc tgctcagcca ggcgctggcc ataggccagt
     4321 aggaggaggt gggcgtgccg tcgttgggat gggtttccct ccagatggaa gtgttgtggt
     4381 gggctaccca gccgttgcga ccgtacattt cgcgtgcggt tttgttgccc gagacggcca
     4441 tctcttttat catctggaaa aagggttcat gacattccgg caaatttccg ggctctgccg
     4501 gccagtaatt catttcggca ttgatgttga gagtgtagcc gcagttccac gagggcatga
     4561 ctttgtcgtt ccagatgcct tgcaggttca taggctggcc gccggggcgc gaggcgctga
     4621 tcatcaggta gcgcccgtat tggtaaagca aagtgatcat accgttatcg tagtcatcgt
     4681 aaaaccggtc gatacgttca tcggtgggca ggcttgccag ttcttttttg taaggcattt
     4741 caaaacttac ccggtcgaac aattgtttat aatcccgaac atgatctttt ttcaggtttt
     4801 cgtagctttt ttgagcggct ttgctcaagg cctcttcggc cagtttgacg gcatccttgc
     4861 cctggcgtga agggcttttg tcgaaaccgt taaagctggt ggcagccgat aatatgaata
     4921 cgatttcgtt tccaccagtc acccgcataa ccttgtcttc ggatacaatt ttggaaccgg
     4981 gagccagaac ttgcaggcga gcttcgtaaa aagtgcccag gccgttgatc tgatctccgt
     5041 agagaacgtt ttcctcgtac ttgcggctgc cgtctgcgaa atagagctcc ggatgcctgc
     5101 cttcggcttt ccagcccctg atttgggcat ctccgcgtct ttgaccgtgt ccgggggctt
     5161 gtccgcttac tttgtatacg ttttcttcaa tgtcgaaaac agtcgtcgcc ctgtgatgcg
     5221 atgtaaaatg cagactgaaa tcaaattcag cttgcttgtc gcaggtcatt ttcatcacga
     5281 tcacctggtc gggatgtgaa atgaacattt cgcgggtgta gttcaggccg ttttgcttgt
     5341 agctcatgcg aaaaaccgag ttggcaatgt cgagttcccg ccagtaatcg ctgatttctc
     5401 cctgggtatg gttggttaaa tgccagtctg ccaagggttg gtaattttcg tgcaaacgtc
     5461 ccagccaatg atattgcaca agttcattgg cctcggcgta cttgccttcg agcagcaaac
     5521 caacgacttt atcgtaagta ggtgtaatat cgtgtacttt ccataccgtg gcgggttcac
     5581 cgctgtagag ggtattatcg ttgagtttga tggtttcgtt cagggcatcg ccgtaaacca
     5641 tggcgccaag ccgcccgtta cccagaggca gggcctcgtt ccagttggcg gccggcttgt
     5701 tgtaccagag gcttaaatcg ttggcaaata cgcaggaaag cgggcagctt aacagcagtc
     5761 ccagaagtaa agcgggcaat ttagatttca tagtcggtgg ttaaggtaat ggagaaagac
     5821 atacattgcc acaaacatac aactattctt tttcccgttg ggttaaaagc tgccaataaa
     5881 taattaaaat cagaaaatcc actttgagca tttaatttta aaatccacga ttaataggtt
     5941 tgggcggtcg cttatttttt ctatcttgcg cagaatttgt tcggaccata catgagaaag
     6001 tccgtttttt gtaaacaatt ttttaaaccg ataaattatg aagaagtatc gttgcactgt
     6061 ttgcaattac atttacgatc cggccgaagg ggatccggat agtgacattg ctccgggaac
     6121 agcttttgaa gatcttcccg acgattggac ctgtcctttg tgcggagttg gaaaagagga
     6181 ttttgagccg gaggattagg ataggatttt acaccatccc caaatctact tattatgaat
     6241 tctaaatttg ttatactatt aacctgtatt attatgagta ttcaaagtgc aggagctaaa
     6301 gctccttttg aaaagcccca gcttaagtat gcccacaatg ccctggaacc ttcgattagt
     6361 gctgagacca ttgaatatca ctatggaaag cacttgcagg cttatattga taacctaaac
     6421 aaattagctg ccgggactgc atttgccgag atgtcgctcg acgacattgt gcttaaagcc
     6481 gaaggcggca ttctgaataa cggagcccag agctggaatc atatttttta tttcgacagc
     6541 ttttcaccca aagccaaaac tgcaccgggc ggaaagcttc tcgaagccat caaccgtgat
     6601 ttcggttcgc tggaggcgtt caaagaacaa ttcaataaag cggccgccac tttgtttgga
     6661 tcgggctggg cctggctgtc ggtagataaa aacggcaaat tggtcataac tgccgaatcg
     6721 aatgccggta atcccatgcg cagcggtctg aagccactgt taaccatcga tgtctgggaa
     6781 cacgcttatt acattgatca tcgtaatcgt cgtccggatg ccattgcagc cttttggaaa
     6841 gtgctcgatt ggtcggtggt agaagctcgc ttcgaaaaca agctctaaga tttacccggt
     6901 caccggataa gccccgaggt atgttcgagc attaaattgc cgggccatga aaccaccgta
     6961 tactatcacc cctttgatac tgaggtctgt gtcttctatt tcggaaaaac tgggagaggt
     7021 caacgccagt catttccgtc gtccttcgtc cagctggaga aagcgaaacg ctttgctaaa
     7081 agtgcatgcc tccctgcgtt tggaagggaa taaaatgagt ctgcggcaga ttaaggctgt
     7141 acttgaacaa agacaggtca aggcagatcc tcaggatatc ctggaagctc aaaatgccct
     7201 gaaggtttat gatgaacttt cgctgatg
//
LOCUS       DAOSGL010000082         7219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8988_length_7219_cov_4.097711, whole genome shotgun sequence.
ACCESSION   DAOSGL010000082 DAOSGL010000000
VERSION     DAOSGL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8988_length_7219_cov_4.097711"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKY68_03910"
     CDS             complement(<1..271)
                     /locus_tag="PKY68_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24214.1"
                     /translation="MKKFTLLFCSLLLAFVASANVIVVDSVSNYATKYGATADGDTLL
                     LYSGTYANQNFPVGKAITILAPDTCEVFLTGEIRGEGVGDGSLLHL"
     gene            464..2128
                     /locus_tag="PKY68_03915"
     CDS             464..2128
                     /locus_tag="PKY68_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOH24215.1"
                     /translation="MNKYLLLILHLCFCASNFSLMADTIPAFPGAEGHGRFTTGGRGG
                     LVYHVTSLEDDPVNPKFGTLRYFLNKTEKRTIVFDVSGTIFLKDALNINAGNLTIAGQ
                     TAPGDGICLAGYPVHVNSSNIIIRFLRFRMGDLDPNNEDDALGGRGIKNIVIDHCSCS
                     WSVDECVSFYGNENLTLQWCIISESLRHSNHEKGNHGYGGIWGGANTSFHHNLLAHHS
                     SRTPRLGAADNTDGRDNVDLRNNVIYNYGLVVGCYGAEGMDANIINCYYKPGPSTKTN
                     HNRRSMIIGIGANTTDSARPKWGKYYIAGNWCSSLSDTYKLPTDSYERNTCLDNWNYG
                     VYNNISEPISAQEKAAMRLSEPNNPGLVTTHTAQVAFEKVLGTAGCSLQRDEIDLRIV
                     DETRRGTATFKGNYTAVGGIIDSQNDLKPAGAGSDWSPWPTLNSTDPPKDTDRDGMPD
                     AWEEARGLNPRHPLDRNDIAENGYTNLENYLNSLVEEIVRQELSDYEFVTGSGQNDLP
                     AAINPPMVAKQKKENNKVWYDLSGRPIESPNARGLYIRRGEKLVIR"
     gene            2151..4559
                     /locus_tag="PKY68_03920"
     CDS             2151..4559
                     /locus_tag="PKY68_03920"
                     /inference="COORDINATES: protein motif:HMM:NF027701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24216.1"
                     /translation="MKIRAFHRKRHAWLPAAFIAAVVSSCNDHMSEYYARPDWIKGNC
                     YEVLEMDGNYTIFLKGIDKAGFHDMVDGKSIVTVMAPNDSAFSLYLAEQGLNSIEEMD
                     SSELDKLIGFHLMYYAYSADKLINFRPEEGDDVTDEEKKINAGMYYKHRTKSKDPNTI
                     EYDNKRQMDVTVFHQERFLPVFSVMYFDTKMIDAAENYNYFFPDVAWNPNNAGFNVAG
                     AAVTQANLITSNGYIHCLDHVLKPLNTIYTELKNDSNFSQFLSLYKKKETFAYNAELS
                     LEYGNGERLYEHLFSQPLPNIASEWPTNDYSKLTELSSVGYSIFAPTNAALNTFFDNY
                     WAKGGYAKLDDVSRESVEKLLYNCVYQGVLKSGGNSYDGNLAFPEEIRRGDIINSYGA
                     VINVPLDAVPQKYRILCENGLLYGCEELAVPTMFAAVTGPAYQYKDFSHYLIMLTSIG
                     DIQNTLWSDETQLLSLMPDNAQIEAMGITYDSRNNYLVRGGKALANDFKISTTYAHLV
                     DLSASTCGDTELDSTSPGKQQVFRAFSPDYIFYWYVMDGKLTNSFRFNERIYPQLKSD
                     QEVFVSFSELPSYKEDGSWSNGKSYTYEGSMFVGMPSETTYKSFQQMMQNNKVDSLLP
                     YHGFVNLMTIAGMYDGAKFNFVQEGENHLMLIPVNSAVFDAIENSAIPGLSFDSAAYG
                     AGSNIFDCCSVADDSSRYVLQMYLKDYFVPLATAGISNYPYLGWKEDTSKGMESLNVI
                     EIYDESTSRVTYQSVKIVITDKGDKLTVRLLESDEEIEVSGDYHYLPFVFEDGCVHFI
                     NGMF"
     gene            4565..>7219
                     /locus_tag="PKY68_03925"
     CDS             4565..>7219
                     /locus_tag="PKY68_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24217.1"
                     /translation="MKKIITLLILCLLLIGTSSAQKTLTGRVYENFGGKEELAVGVNI
                     SFNNAQNRMVEGTVSNSNGEYSLRVPLGGGPYTVVFSYIGMKTQSIPYNGQSSLNVVL
                     EEDAKALDEVVVAAKQEQKNEMGITLREQTAATERIQLSEIIEFTPVTSVEEALQGRL
                     AGVDILTGGDPGARNSIRIRGTATLNTSADPLIVINGVPYSTDIDDSFNFATASNEDY
                     AAMLNLSPYDIESIEVLKDAASTAIYGTSGASGVLLITTKTGSKGKPRFTLSSKFTAK
                     FEPESIPMLNGKEYVAFIEDAIWNTANAKGVSTSAALLELLYDTPEINFNKEWRYFNE
                     YNTNTDWLAAVVQNAYTTDNNFSMSGGGEKATYRFSLSYADEDGTTIGTNMNRFSSTL
                     NIGYRFNDKLRVETDFSYAETDKKANWTDNVRSEAMLKMPNKSPYWIDDITLTPTDRY
                     FNRQNAEEFQGAFTGSKNFHPIAMANESFNNSLQQEEKMNFRLNYDILPGLIYTGYVS
                     MKFKTIKNRAFLPQSATGVTMDNTYANRSSDAYSDNLALQTENKLIFRRNWNGIHNIV
                     ATALWRTSDSQSSNYSNTIYGVASKHASDPSTNGAIVERGSGDSQVRSISGIGGINYT
                     FLNRYILSGTVNYEGKSSLGKANRWGLFPSLGLAWHIKEENFLQNADWLSSMKLRCSY
                     GQSGAAPSGTAPYMGTYSALGDGYRDNPSIVPVSVQLNKLKWQTASEYDAGFDAGFLD
                     QRLTATFDIYYKYITDLLQRRIAIPTTSGYNSQGNSIAYYNSGEMSNTGWEFRIDYTV
                     VQNDDWRITLNYNIARNINRIEKLPENLPESSYSLRNGTYAQRIIVGAPVGSFFGYRY
                     LGVYDTTLETYARDARGAVMTDLDGKPIV"
BASE COUNT         2063 a         1825 c         1654 g         1677 t
ORIGIN      
        1 cgagatggag taacgaaccg tcgccaactc cctcccccct gatctctccg gtcaggaaaa
       61 cttcgcaggt gtcgggagct aaaatggtga tagctttgcc aacagggaaa ttttgattgg
      121 cataagtgcc gctgtacaat agcagtgtgt cgccgtcggc cgttgccccg tatttggtag
      181 cataattgga cacgctgtcc acgacaatta cattggctga agccacaaaa gcaagcagca
      241 atgaacaaaa caaaagagta aattttttca tagtcggtta atttaatagg tttagtagtc
      301 aattaccacc aaaaataaag catcgaaatt ttctcttagt ggattatttg ttgagatagg
      361 tgtacttttt aaacaaaaaa tatcctccta ttccgcaaaa aagtacaccc caaggccact
      421 taagcaaggt atttttgttt cgattataat aaacccgata ccgatgaaca aatatttatt
      481 gctgattttg catttatgct tctgcgcatc gaatttctcg ttgatggccg acactattcc
      541 cgcttttccc ggggctgaag gccacggacg ttttaccacc ggcggacgag gaggccttgt
      601 gtatcatgtt accagcttag aagacgatcc tgtaaatccc aagttcggca ccttgcgcta
      661 ttttctgaat aaaaccgaaa aacgaaccat cgtttttgat gtatccggta ccatcttttt
      721 gaaagacgcc ctcaatatca atgcaggcaa cctgaccatt gccggacaga cagcaccggg
      781 tgacggtatt tgcctggccg gttatccggt tcacgtcaat tcgagtaaca ttattattcg
      841 ttttctgcgt ttccgcatgg gcgacctcga tcccaacaac gaagacgatg ctttgggagg
      901 aagaggcatc aaaaacatcg tgatagacca ctgctcctgc tcctggagcg tagatgaatg
      961 tgtttctttc tacgggaacg agaatctcac gctgcaatgg tgtattatca gtgaaagcct
     1021 gcgtcattcc aatcacgaaa agggcaatca cggttacgga ggcatctggg gaggagccaa
     1081 cacttcgttt catcacaacc tgcttgccca tcacagcagc cgcacacccc ggctgggcgc
     1141 ggcagacaat acggacgggc gcgacaacgt agaccttaga aataacgtta tttacaatta
     1201 cggtctggta gtaggctgtt acggagccga aggcatggat gccaacatca ttaattgtta
     1261 ttacaaaccg gggccttcca ccaaaaccaa ccacaaccgc cgtagtatga taatcggcat
     1321 cggagccaac accaccgaca gtgcccggcc gaaatggggg aaatactaca ttgccggcaa
     1381 ttggtgttct tccctgagcg acacttataa attacccacc gacagctacg agcgcaatac
     1441 ttgtctggac aactggaatt acggagtgta caacaatata tcggaaccta tcagtgccca
     1501 ggaaaaagct gcgatgagac tgagcgaacc caacaatccg ggcctggtta caacccatac
     1561 cgctcaggtc gctttcgaaa aagtattggg cactgcaggc tgttccttac aacgggatga
     1621 aattgacctg cgtattgtgg atgaaacccg ccgaggtacc gctactttta agggcaatta
     1681 cactgcagtg ggtggtatca tcgacagcca gaacgacctt aaaccggccg gtgccggcag
     1741 cgactggagc ccctggccca ccttgaacag cacggacccc ccaaaagaca cagatcggga
     1801 tggtatgccc gacgcctggg aagaagcacg gggacttaat cccagacatc ctctcgatcg
     1861 caacgacatt gccgaaaacg gctacactaa cttagaaaac tacctaaata gtttagtcga
     1921 agaaatcgtc cgtcaggaac tttcggacta cgaattcgtt accggttccg gtcaaaacga
     1981 tctgcccgca gccataaatc ctcccatggt cgcaaaacaa aaaaaagaga acaataaggt
     2041 ttggtacgac ctgagcggcc gaccaatcga gtcgccaaac gcaagaggat tgtatatacg
     2101 ccgtggagaa aaattagtca ttcgatagcc aacaaaacat caataaaacc atgaaaatca
     2161 gagcattcca tagaaaaaga cacgcatggc tgccggcggc attcatcgct gcggttgtaa
     2221 gcagttgcaa tgaccacatg tcggaatatt atgctcgtcc cgactggatc aagggaaact
     2281 gctacgaagt cctcgaaatg gatggcaatt ataccatttt tctgaaagga atcgacaagg
     2341 caggttttca cgatatggta gacggcaaaa gcatagtaac ggtaatggca ccgaacgatt
     2401 ctgcctttag cctctatttg gctgaacagg gactgaacag catagaggaa atggattcct
     2461 ccgagctgga taaacttatt ggtttccacc tgatgtacta tgcatacagt gcagataaac
     2521 tcatcaactt tcgtcccgaa gagggcgacg atgttacgga tgaagagaaa aaaatcaacg
     2581 ccggcatgta ctacaagcat cgaactaaaa gcaaggatcc caataccatc gagtacgaca
     2641 ataaacgtca aatggatgtt actgtctttc accaagagcg ttttttgcct gtgttctcgg
     2701 tcatgtattt cgacaccaag atgatcgatg cggcagaaaa ctataactat ttttttcccg
     2761 atgtggcatg gaatcccaac aatgccggat ttaacgtggc gggcgcagca gtcacacagg
     2821 ctaatctgat tacgagcaac ggctacattc attgcctgga tcatgtactt aaacccttga
     2881 ataccatcta taccgaattg aaaaacgaca gtaatttcag tcagtttctg agcttgtaca
     2941 aaaagaagga gaccttcgcc tacaacgcgg agttatctct ggaatacggg aacggagagc
     3001 gtctgtacga acacctgttc tcccagccct taccgaatat tgccagtgaa tggccaacga
     3061 acgattactc aaaattgacg gaactctcct cggttggtta cagtattttt gcacctacca
     3121 acgctgctct gaatacattc ttcgacaatt actgggccaa aggcggctat gcaaagctgg
     3181 acgatgtcag ccgtgaatcg gtcgagaaac tgctttacaa ttgcgtttat cagggagtcc
     3241 tcaaaagcgg cggcaactct tacgacggca atttagcctt ccccgaagag attcgccgcg
     3301 gagacatcat caatagttac ggtgccgtta tcaatgtgcc tctcgacgct gttccccaaa
     3361 aataccgcat actctgtgaa aacggactgc tctacggctg cgaggaatta gctgtcccca
     3421 ccatgttcgc cgccgtcacc ggcccggcct atcaatacaa ggatttcagt cattacctta
     3481 tcatgctcac ttccataggt gacatccaaa acaccctttg gagcgacgag acacaattgc
     3541 tctcgctgat gcccgacaat gcccagatag aggccatggg cataacctac gacagccgca
     3601 acaattacct ggttcgcggt ggtaaagcct tagctaatga ctttaaaatc agtacaacct
     3661 atgcccacct ggttgatctt tcggcgagca cctgcggcga tacggaactc gactctacaa
     3721 gcccgggcaa acaacaagtg ttcagggctt tttcgcccga ttacattttc tactggtatg
     3781 tgatggacgg taaactgacc aacagttttc gtttcaacga gcgcatctat ccacaactta
     3841 aatcagacca ggaggtgttt gtttctttca gcgaattgcc atcctacaaa gaggacggca
     3901 gctggagcaa cggcaaatcc tatacctacg aaggatccat gtttgtcggc atgccttcgg
     3961 agaccaccta caagtcgttc cagcaaatga tgcagaacaa caaagtagac tccctgctcc
     4021 cctaccatgg ttttgtaaac ttaatgacta tagccggcat gtacgacggg gcaaagttta
     4081 atttcgttca ggagggagaa aaccatttaa tgcttattcc cgttaactct gccgtgttcg
     4141 acgccatcga aaactcggcc atccccggat tgagtttcga ttcggctgca tacggcgcgg
     4201 gaagcaatat tttcgactgt tgctcggtgg ccgacgacag ttcaagatat gtactgcaaa
     4261 tgtatttgaa agactacttc gttccgcttg ccacggcggg catcagcaat tatccctatc
     4321 tcggctggaa agaagacacc tccaaaggca tggaatccct gaacgttatt gaaatttacg
     4381 atgaaagcac cagccgggtt acttaccagt cggtaaaaat tgtgattacg gacaagggag
     4441 ataaactcac tgtccgtctg cttgaaagcg acgaagaaat cgaggtatcg ggagattatc
     4501 actatctgcc ctttgtgttt gaagatggtt gtgttcattt tattaacggc atgttctgaa
     4561 tagtatgaaa aaaataatca ccctattgat tttatgcttg ctgctgatcg gcacaagctc
     4621 agctcaaaag acgctgacgg gacgtgtcta tgaaaacttt ggaggtaaag aagagcttgc
     4681 cgtaggtgta aacatctcct tcaacaacgc tcaaaaccgt atggtcgaag gaacagtctc
     4741 caacagcaac ggcgaataca gtctgcgtgt cccattggga ggagggcctt ataccgtggt
     4801 cttttcctac atcggaatga aaacccagag tattccctat aacggtcaat cttcgttgaa
     4861 cgtagtgctc gaggaagacg ctaaagcact ggacgaggtg gtggtagcag ccaaacagga
     4921 acaaaaaaac gagatgggca tcaccctgag agaacaaacg gccgctaccg aacgtataca
     4981 actaagcgaa attatagaat ttactcctgt cacttcggtg gaagaagcat tacagggccg
     5041 tctggccggt gtggatattt tgaccggagg cgatccggga gccaggaatt ccattcgcat
     5101 tcgcggtaca gccacactca ataccagtgc agaccctttg attgtgatca acggtgtccc
     5161 ctattcgacc gacattgacg attccttcaa ttttgctacg gccagtaacg aagactacgc
     5221 tgccatgctg aacctgtcgc cttacgacat cgaaagcata gaagtattga aagacgccgc
     5281 ctccacggcc atttacggca catcgggcgc cagcggtgta ctgctgataa cgaccaaaac
     5341 aggatcgaaa ggcaaaccgc gtttcactct gtcttcgaag ttcacggcca aattcgagcc
     5401 ggaatcgatt ccgatgctca acggtaagga atacgtggcg tttatcgaag atgccatctg
     5461 gaatacggcc aatgccaaag gcgtttcgac cagtgccgct ctgctggagc tgctgtacga
     5521 tacgccggaa attaacttta acaaggaatg gcgctatttc aacgaataca acaccaacac
     5581 cgactggctg gccgcggtag tacagaatgc ttatacaacg gacaacaact tttcgatgag
     5641 cggcggaggt gaaaaagcca cctatcgttt cagtctttct tacgccgacg aggacggaac
     5701 taccataggg accaacatga accgtttttc cagtacactc aacatcggct accgcttcaa
     5761 cgacaaattg agggtggaaa ctgacttttc gtatgccgaa acagacaaga aagcgaactg
     5821 gaccgacaat gtccgttcgg aagccatgct gaaaatgccc aacaaaagtc cctattggat
     5881 agacgatatc acgttgactc ccaccgatcg ttacttcaat cgccagaatg cggaggaatt
     5941 tcagggagcc tttaccgggt ccaaaaactt ccatcccatt gccatggcca acgaaagttt
     6001 caacaacagc ctgcaacagg aagaaaaaat gaactttcgc ttgaactacg atatcttacc
     6061 gggtcttatc tacacaggat atgtttcgat gaagttcaaa accatcaaaa acagagcctt
     6121 tttaccccag agtgccacag gagtcactat ggacaatact tacgccaatc gcagcagcga
     6181 tgcctacagc gacaacctgg cactacagac cgaaaacaaa ttgattttcc gccgcaactg
     6241 gaatggtatt cacaacattg tggctactgc tttatggcgc accagcgaca gccaaagcag
     6301 taactactcc aataccattt acggcgtggc ttccaaacat gcttccgatc catcgaccaa
     6361 cggggccata gtagaaagag gttccggcga ttcacaagtt cgctcgatca gcggcatcgg
     6421 tggtataaat tatacttttc tcaaccgtta cattctttcg ggaaccgtca actacgaagg
     6481 taaatcgtcg ctgggtaaag ccaaccgctg gggattattt ccttccctgg gccttgcctg
     6541 gcatatcaaa gaggaaaatt tcctgcaaaa cgccgattgg ctgagttcca tgaaattacg
     6601 ttgcagttat ggccagagcg gcgccgctcc ttccggaacg gctccttaca tgggaaccta
     6661 ttcggccctt ggtgacggtt accgcgacaa tccttccatc gtacccgtat cggtgcaatt
     6721 gaataagcta aaatggcaaa cggccagtga gtacgacgcc ggttttgacg ccggtttttt
     6781 ggatcaaaga ctcacagcca ctttcgacat atattacaaa tacatcaccg atttgcttca
     6841 gagaaggatc gccattccca ctaccagtgg ttacaactct cagggcaaca gcattgccta
     6901 ttacaattcg ggagaaatga gcaataccgg ctgggagttc agaatagatt acacggttgt
     6961 tcaaaacgac gactggcgca tcacgctcaa ttacaacata gcccgcaaca tcaaccgcat
     7021 cgaaaagtta ccggaaaacc tgcctgaaag cagttacagt ttaagaaacg gcacctacgc
     7081 acagcgtatc atcgtaggcg cacccgtcgg cagcttcttc ggttaccgtt acctgggggt
     7141 ttacgacacc actttggaaa cttatgcccg cgatgcccgg ggagccgtta tgaccgacct
     7201 cgacggcaaa cccatagta
//
LOCUS       DAOSGL010000083         7214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_8999_length_7214_cov_4.100852, whole genome shotgun sequence.
ACCESSION   DAOSGL010000083 DAOSGL010000000
VERSION     DAOSGL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8999_length_7214_cov_4.100852"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..2098
                     /locus_tag="PKY68_03930"
                     /pseudo
     CDS             1..2098
                     /locus_tag="PKY68_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225820.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
     gene            2102..2752
                     /locus_tag="PKY68_03935"
     CDS             2102..2752
                     /locus_tag="PKY68_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like putative outer membrane lipoprotein
                     chaperone"
                     /protein_id="HOH24218.1"
                     /translation="MSGPKKTLPGLLFLLLGIWAIHAQNQALTILEKAGEAYNKTSGV
                     HAEFSIEMFESGGESAAQIQGQIQLKGDKFKLDVGEMITWFDGKNQWFYLVNIQEVNL
                     SNPAPEDLMMINPVMVFQLYKYGFDTRYAGEKKLGTKIAQQVELLPQEPYSDIQRIEV
                     SFDKQTYQPLRIRIRNKDQSGSLINIDKYITDQKYPDAMFVFQQKAYPGAVVIDLR"
     gene            2757..3689
                     /gene="trxB"
                     /locus_tag="PKY68_03940"
     CDS             2757..3689
                     /gene="trxB"
                     /locus_tag="PKY68_03940"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOH24219.1"
                     /translation="MEHVRCLIIGSGPAGYTAGIYAGRAGLKPLLFEGMQIGGQLTTT
                     TEVENFPGYPQGVSGFQLMEDLRNQAQRFDCECRNGTITAADLSQRPYVLTVDDETKI
                     SCDSLIIATGATAKYLGLADEQKYAGMGVSACATCDGFFYRQKTVAVVGGGDSACEEA
                     LYLSTLASKVYVVVRKNYLRASKIMQERTLNNPKISVLFEHEVVGLYGDNGVEGAHML
                     KKRGQTGESRFDLPIDGLFLAIGHKPHSDIFAPYLKVDKTGYIITEPNTPKTSLPGVF
                     AAGDVADSVYRQAVTAAASGCKAAIEAERYLNEL"
     gene            3821..4135
                     /gene="rplU"
                     /locus_tag="PKY68_03945"
     CDS             3821..4135
                     /gene="rplU"
                     /locus_tag="PKY68_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOH24220.1"
                     /translation="MYAIVDIAGQQFKAEAGRRLYVHRLEAEQGSVVEFDKVLLVDNE
                     GAVQVGTPVVEGAKVVAEVLSHLKGDKVIVFKKKRRKGYRKKKGHRQYLTEVKINEIV
                     VA"
     gene            4156..4419
                     /gene="rpmA"
                     /locus_tag="PKY68_03950"
     CDS             4156..4419
                     /gene="rpmA"
                     /locus_tag="PKY68_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOH24221.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIFGGQFAKAGNILVRQRGTV
                     HHPGDNVGIGKDHTLFALVDGTVTFKRKKNDRSFVSVLPQEEA"
     gene            4555..5079
                     /locus_tag="PKY68_03955"
     CDS             4555..5079
                     /locus_tag="PKY68_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOH24222.1"
                     /translation="MISSLSFKALNLSFTNYRTYLFGAAFVAGNLLLPQLCHLMPNGG
                     LIWLPIYFFTLIAAYKFGLKIGLFTAALSPLVNYLLFGMPPLGMLPIILIKSGLLAFA
                     AAFVASKTNKLSLLHLLVVILAYQVLGGLAEWGLSGSFAAALQDFKLGLPGLLVQLLG
                     GWLVLKALAKYELK"
     gene            5309..6205
                     /locus_tag="PKY68_03960"
     CDS             5309..6205
                     /locus_tag="PKY68_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH24223.1"
                     /translation="MFQKLVAIEPVSLIPSAEKKLSDFAKEVVLYDTIPDSDREIARR
                     IGDADAVLLSYTTHLGKGALEQCKHLKYIGMCNSLYSPESANVDIYYANSRGITVTGI
                     RDYGDEGVVEYVISELVRCLHGFDQVPWDGMAREITGLKAGILGLGKSGGMIADALHF
                     FGAEISYFARSEKEAARQKGYRFLPLNDMLRESEVVCCCLNRNTVLMQARQFEALGNK
                     KILFNTGLSPAWEEAPFLRWLEGDNLCFCDTVGALGGPHLLDHPKVRCMQVSTGRTRQ
                     AFVRLSEKVLQNLTDFTGIKPD"
     gene            complement(6202..6990)
                     /locus_tag="PKY68_03965"
     CDS             complement(6202..6990)
                     /locus_tag="PKY68_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF108 domain-containing protein"
                     /protein_id="HOH24224.1"
                     /translation="MKKLVIFGSGYLANIVADAYLDGLLPEYELVGVYSRTIDKAKRL
                     ARKIAQSGRDCRPCVTLNALLSLKPDYLVETASPAAMKRLALPTLNNGTSIITLSIGA
                     FADTAFYEQVKQSAAANDVRVYIASGATGGFDVLRTATLMGQASANFLNIKGVRALRR
                     SSFYEPGMETEAKTVFTGTAREAIDVFPTNLNVAVAASLATVGPENLQVSMISTPEFT
                     GDTQRVEIKNKEVHAVVDVFSATPAIAAWSVVSTLQNIVSPIVF"
BASE COUNT         1947 a         1747 c         1745 g         1775 t
ORIGIN      
        1 tacccccaat ctgggcaaag ttttttttgt gtgctgtttc gcactcatct ggctctcctt
       61 atttttcggt ttctttttta tccgcgaaga aagcagccaa tttttgtttt tgggaggagc
      121 tcacggcttt tacatcagtc gctggcttca ggaaagcatt ggttggatag gtacatttct
      181 gttccttttg cttagtttga tcgtcttttt agtgctcacc agcaaaaaaa ccattcccct
      241 gctgcgctcc ctcttcaaaa tggagtattt aaaaaataag caggccgaat tggcggatac
      301 catggaagaa gaagctgcca cggaaggccc gaaagacttg ggttatacca aaccgcccct
      361 tgccgacgat ccccaaactg aagaaaatgg agaattgcct tctaccaatg aggaagagga
      421 aaaatccccc ggacagaaaa cccgggccgg taaggacatc gatatggata ttatcgtacc
      481 aaaaggcgac gagaatatca atgccgatga agaatcagac gacgaggaac tgctggaaca
      541 actccgcaaa aagaaaaacg aagaatacga tgccctggcc gaattgggcg aatacgaccc
      601 tagggccgag ctgagcaaat tcaaaaatcc gcctccggaa attttaaagc aatacgacga
      661 ccgaacggtg gtaaaccacc aggaacaaac ggaaaataag cgtaaaattg tcgatgtact
      721 cgaaagtttc ggcatacgaa tttccagcat tcaggccacg gtggggccca ccgtcacctt
      781 gtacgaaatc gtaccggaac ccggaattcg catcaccaag atcaagggac tcgaaaacga
      841 tatcgcactg aatctggcag cccttggtat tcgcatcatt gctcccattc cgggcaaagg
      901 caccatcggt atagaagtgc ccaaccgcga tcctaaactg gtatctatgt tcagcctggt
      961 gaaatcgcgc aaatttcagg agaccaaata cgacctgccc gtggtgctgg gtaaaaccat
     1021 tatgaacgaa gtgtttatgt tcgacctctg taaaatgccg catatcctgg ttgcctgagc
     1081 caccggacaa ggaaaatcgg tggggttgaa cgcattgatt acttcccttt tgtacaaaaa
     1141 acatcccgcg gaactcaagt ttgtcttggt cgatcccaag atggtggaat tcagtatcta
     1201 ttctcccatc gaaaagcatt acctggccaa actgcccggt gacgaaaaca aggccatcat
     1261 caccgactac accaaagtcg tccagaccct gcattcgctc accacagaaa tggacgatcg
     1321 ttacaagctg ttgaatatgg ctcatacccg caatatcaag gaatataacg ccaagtttat
     1381 agctcgccgg ctcaatcccg aaaaaggcca taaatacatg ccttacattg tcatcgttat
     1441 cgacgagttc ggagacctga ttatgacggc cggcaaagag atcgaactcc ccattgcccg
     1501 tattgcccaa aaagcccgtg ccgtaggtat gcacatggtg attgccactc agcgtcccac
     1561 aaccaatatc ataaccggaa ccatcaaggc caacttccct gccaggctgg catacaaagt
     1621 atcttccgcc acagactcca ggaccattct cgacgccaac ggcgccaacc agcttatagg
     1681 caagggagac tccctgtttt cacagggcaa cgatatgacc cgtatccagt gcgcctttat
     1741 tgatacgccc gaggtcatcg atatggttga atacatcagc agccagcgcg gatatcccac
     1801 cgcctttgaa ctgcccgaat acgtggacga aagcagcgga ggcggcctga gcgatgtaga
     1861 cctcagcctg cgcgatcccc ttttcgaaga agccgcccgt ctgatcgtga ttcaccagca
     1921 gggatcgact tcgctgattc agcgcaaatt ctcgataggc tacaaccggg ccggacgcat
     1981 tgtcgaccaa ctggaagccg ccggcattgt cggtcccagc gaaggcagca aggccaggca
     2041 ggtactggta gatgatgaac accacctgga tctgctctta tccaatttaa atccttaaaa
     2101 catgtcagga ccaaagaaaa ccttgcccgg cttgttgttt ttactcctgg ggatatgggc
     2161 catccacgct caaaaccagg cccttactat cctggaaaaa gccggcgaag catacaacaa
     2221 aaccagcgga gtacacgctg aattcagcat agagatgttt gaatccggcg gagaaagcgc
     2281 tgcccaaatt caaggccaaa ttcaactgaa aggcgataaa ttcaaactgg atgtaggcga
     2341 aatgattacc tggttcgacg gtaagaatca atggttttat ttggtaaata ttcaggaagt
     2401 aaacctaagc aatcctgctc ccgaagacct gatgatgatc aatcctgtta tggtgtttca
     2461 gctatataaa tacggttttg acacccgtta tgcaggagaa aaaaagcttg gcacgaaaat
     2521 tgctcaacag gtggaattgc tccctcagga gccctattcg gatattcagc gtattgaggt
     2581 cagtttcgac aagcaaactt accaacccct gcgcattcgt atccgtaata aagaccaatc
     2641 gggatctttg attaatattg ataaatacat aaccgaccaa aaatacccgg acgctatgtt
     2701 tgtctttcag caaaaagcct atcccggtgc cgtggtcata gatttgaggt aaaattatgg
     2761 aacatgtacg ttgcctgatt atcggatcgg gccctgccgg ctataccgcc ggaatttacg
     2821 ccggccgggc cggactaaag cctttgttgt tcgaaggcat gcaaattggc ggacaattga
     2881 ctactaccac cgaagtagaa aactttcccg gctacccaca gggagtgagc ggctttcaac
     2941 ttatggaaga tcttcgcaac caggcccaac gtttcgattg tgaatgcagg aacggtacga
     3001 taacagcggc cgacctctcg caacgcccct atgtacttac cgtagacgac gaaactaaaa
     3061 tctcatgcga tagtctgatc attgccaccg gagccaccgc caaatacctg ggcctggccg
     3121 atgaacaaaa atacgcaggt atgggtgttt cggcctgcgc cacctgtgac ggcttttttt
     3181 accgtcaaaa aaccgtggcc gtggtgggcg gaggagattc ggcctgcgaa gaagcccttt
     3241 atctgtcgac tctggcctcg aaagtgtacg tagtggtgcg caagaattac ctgagagcct
     3301 ctaagataat gcaggaaaga acgttgaaca atcccaaaat cagcgtatta tttgaacacg
     3361 aggtggtcgg tctttacgga gacaacggag tggagggcgc ccatatgctc aaaaaacggg
     3421 gacagaccgg tgaaagccgt tttgatctgc ctatagacgg tttgttttta gccataggac
     3481 ataaacctca ttccgatatc tttgctccct atctcaaagt ggacaagaca ggttatataa
     3541 tcaccgaacc caacacaccc aaaacaagtc tgcccggtgt ttttgccgcc ggcgacgtgg
     3601 ccgattccgt ttaccggcag gctgttacgg cagcagcttc gggttgcaaa gctgccatcg
     3661 aagccgagcg ttatttgaac gagctgtaaa atatttcaag tttaaacttg gcagaattaa
     3721 aaaaaagggc tacctttgcg gctcgatttc gtgtaaggta aaaagcggca ctgccctggc
     3781 atcccacctt ccgaagaata acttgtaaat tagttagtta atgtatgcaa ttgtagacat
     3841 tgcgggacaa cagttcaagg ccgaagcagg ccgcaggctg tatgtacatc gcctggaagc
     3901 agaacaaggt tctgtggtag agtttgataa agttcttttg gtcgacaacg aaggagccgt
     3961 acaggtggga acacctgtcg tagaaggtgc caaagtagtg gccgaagtcc tttctcacct
     4021 caagggagac aaggtaattg tcttcaagaa gaaaagaaga aaaggctaca gaaagaaaaa
     4081 aggtcatcgt caatacctga ccgaagtgaa aatcaacgaa attgtagtcg cttaatcctt
     4141 taaattttac agattatggc acataaaaaa ggagttggta gttccaagaa cggccgcgaa
     4201 tcggaaagca aacgattggg cgttaagatc tttggcggac agtttgccaa agcaggaaat
     4261 attttggttc gtcagcgcgg cacggtgcat catccgggcg ataacgtagg tatcggtaaa
     4321 gaccatactc tctttgcctt ggtcgacggc acggtaactt ttaagcgcaa aaaaaacgat
     4381 cgttcctttg tttctgttct tccccaggaa gaagcataaa ggtttttaaa cgataatatc
     4441 acttaaagcg ggcaaattat tgcccgtttt tttgtgttgt ttcgttcgct tgcttatatt
     4501 tgtcgtccgt ttaagcaatt gcaatcgttt gtataacaaa taaatttcaa tgatatgata
     4561 tcctcactga gttttaaagc cctcaattta agctttacca attatcgcac ttatttgttc
     4621 ggtgccgcct ttgtagccgg caacctgctt ttgcctcagc tttgccactt gatgcccaac
     4681 ggtggtttga tctggctgcc catttatttc ttcacactga ttgccgccta taaatttggc
     4741 ctgaagatcg gcttatttac ggcggccttg tctcctcttg tgaattacct gctttttggt
     4801 atgccgcccc tgggtatgct gcccatcatc ctgattaaat cgggtttgct ggcttttgcc
     4861 gctgcctttg ttgcctctaa aaccaacaaa ctttcgttgt tgcatttgct ggtggtaatt
     4921 ctggcttatc aagtattggg aggtctggct gaatggggac tgagcggaag ttttgccgcc
     4981 gcattgcaag atttcaaatt aggtctaccc ggtctgctgg ttcagctctt aggtggctgg
     5041 ttagtgctga aagcattggc aaaatacgaa ctgaaataaa gaactgaaat aaaaagcgca
     5101 agctaaaata aaaagtacaa actgaaacaa ccggtttgtt tacacaagaa attaaagtgg
     5161 ctgtcgattt gatggccact tttttgatga cgattatcca cctatgtgaa ctttcacatt
     5221 caactctgct ccccgccgga ttagcaactc cggtctgagt tttaaatgct atctttgcag
     5281 cattataaac gttttagaat tccagcctat gttccaaaaa cttgtggcca ttgagccggt
     5341 tagtctgatt ccttcggccg aaaaaaaact gtctgatttt gccaaagaag ttgttttata
     5401 cgatacgatt ccggattccg accgggaaat cgcgagacgt atcggcgatg ccgatgccgt
     5461 attgttgagc tataccaccc atctcgggaa aggggcacta gagcagtgca aacacctgaa
     5521 gtacatcgga atgtgcaatt ccttgtattc gcccgaaagt gccaatgtgg atatttatta
     5581 cgccaacagc cgggggatca cggtgacggg aatacgcgac tatggcgacg aaggcgtggt
     5641 ggaatacgtc atcagtgagt tggtgcgctg cctgcacggc ttcgatcagg taccctggga
     5701 cggcatggcg cgtgaaatta cgggtttgaa ggcgggcatt ctggggctgg gcaagtcggg
     5761 cggcatgatc gccgatgcct tgcatttttt tggcgcggaa atcagctatt ttgcccggtc
     5821 ggagaaagaa gcagcccggc agaaaggcta tcgttttctg cccttgaacg atatgctaag
     5881 agagagtgag gtggtgtgct gttgcttaaa ccgaaacacc gtcctgatgc aagcgcgcca
     5941 gttcgaagct ttgggcaaca agaagatact gtttaacaca gggctttcac cggcctggga
     6001 agaagcgccc tttttgcgtt ggttagaggg cgacaatctt tgtttttgcg acacggtggg
     6061 tgccttgggt ggccctcacc tgctcgatca ccccaaggta cgttgtatgc aagtatccac
     6121 agggcgcacg cgtcaggcct ttgtccgctt gagcgaaaag gtcttgcaaa atttaaccga
     6181 ctttacaggg attaagcccg actaaaagac aatcgggctt acgatgtttt gcagggtact
     6241 gaccacagac caggcggcga tggccggtgt ggcactgaat acgtcaacca ctgcatgcac
     6301 ttccttattt tttatttcca cccgttgtgt atcgcctgtg aattcggggg ttgaaatcat
     6361 actgacctgc aggttttccg gacccaccgt agccagcgat gcggcaacag ctacattcaa
     6421 gttggtggga aacacatcga tggcttcgcg ggcagtgccg gtaaagacgg tcttggcttc
     6481 cgtttccatg cccggttcgt agaaagacga ccgtctgagg gctctaacgc cctttatatt
     6541 aagaaaattt gctgaggcct gacccatgag agtggctgtg cgtaagacat caaagccacc
     6601 tgtggcgccg gaagcaatat acacccggac atcgttggcg gcagcactct gcttcacctg
     6661 ctcataaaaa gcggtgtcgg caaaggcacc gatggaaagg gttatgatgg aagtaccgtt
     6721 attgagcgtt ggcagggcca gtcgcttcat agctgccgga gaggccgttt cgaccagata
     6781 gtcgggcttc agagacagta aagcattgag ggtgacgcag ggccggcagt cgcgtcccga
     6841 ctgagcaatt tttcttgcca gacgtttggc cttgtcaatc gtacgggaat aaacacccac
     6901 caattcatac tcaggcaaaa gcccgtccaa ataagcatct gctacaatat tggctaaata
     6961 gccgcttcca aaaatgacca attttttcat gcccgcaaag atacgacctt tagggcaaat
     7021 ttgccagccg cgatcaaatg aacctcctga aaaaattcaa gaacagccag ctcgaaagcc
     7081 cggctcattg attatccaac tcttccgttt ttagctgctg tccggacata ttgtagattt
     7141 tatcttcttt gacaattaca atctgtccat tcagtaggcg cttgaccggc tcaggtgctt
     7201 tgatttctac cacc
//
LOCUS       DAOSGL010000084         7201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_9026_length_7201_cov_3.710467, whole genome shotgun sequence.
ACCESSION   DAOSGL010000084 DAOSGL010000000
VERSION     DAOSGL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9026_length_7201_cov_3.710467"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1442
                     /locus_tag="PKY68_03970"
     CDS             138..1442
                     /locus_tag="PKY68_03970"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOH24225.1"
                     /translation="MKKHLFPLFVLLLLTAGCKNAVKPPQSFDYQPDLEAMGIEAEQI
                     QALDSVLQSFVDAQKLNCLAAFAAKGGKVIYYKAFGWKDLENRVPASVDDYYVLFSQT
                     KAITTVAFMTLVEQGLVAIDDKVADYFPEISDQVVTKVNEDGSYETRPVETPMTFVHL
                     MSHTSGLNAGQVGAIRRAERAQMRRADAASGAEAGQRSFDADPRSGTLEDEMIKLAKY
                     PLGFDPGSEWNYHISTNMLAYMVERISGKPLRQYVKETILEPLGMDHTDWYYPEEALG
                     RFVLPGSIVEGKLVAGTDMFVRGAVSRQQSYCEGAIGLNGPIEDYAKFCQMMLNKGEF
                     NGRRILKPETVELMTTINRLPENNSGGGGFQFGLGFELYNENKKPVPAVSNTAYAWGG
                     MLGTEYIIDPENDLIVLYYTNMFRNERLYPVYLAAVYDLLLP"
     gene            complement(1509..2168)
                     /locus_tag="PKY68_03975"
     CDS             complement(1509..2168)
                     /locus_tag="PKY68_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH24226.1"
                     /translation="MKKMYLLVLLKVFSFSTISFAQVTREQADQIVYKYVTDEELRMD
                     YLLIYTIDSLSNADGVSTITNSNNETFGVDYPSWVYHINEWMDVNGPYFRRYLFIDKT
                     TGNILEVKTRRDFGPSDPGLEKWQLIYQEASGISGINGNLDESYFPNPTGDFLEITCK
                     KDFESIAIFDLEGKPVFQKTFKQLESNQKFDVSFLHRGVYFVNIFDANKKVLSFKIFK
                     R"
     gene            2427..3518
                     /locus_tag="PKY68_03980"
     CDS             2427..3518
                     /locus_tag="PKY68_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOH24227.1"
                     /translation="MPWFLFSFGILNIYDMNRENVITNSKALFLAVILVSTLLFGSCS
                     KSKKPRYEAYLFAYFEGSAGDRQHQEQLRFALSSDAVHWKALNNNEPILISEEISRSG
                     GIRDPHILRAEDGKTFYLVATDMSTGRNGWRSNPGIVMLKSKDLIHWEHSTVHLSKDY
                     PEHFADAYWVWAPQTIYDPAAGKYLVYFTLKLENDDKLDFYSAYANKDFSGFEAEPSL
                     MFSAKHGAIDADIVFKDGTWHMFYKGNTKDEQGREFENGIKQATAPSLQGPWTEHFEY
                     LDVYHNTRTNVEGSSVFKLNDSDTYILMYDLYSSGRYEFQRSQDLYRFTEKPESFVKD
                     FHPRHGSVISITGKEAERLNRKWGGTALE"
     gene            3583..>7201
                     /locus_tag="PKY68_03985"
     CDS             3583..>7201
                     /locus_tag="PKY68_03985"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24228.1"
                     /translation="MSKKINFLVLLAFLTAHVFAQHSLEKLDRGLVAVKISNGVFLSW
                     RIPGEEWHYTTYNVYRDGTKLNNEALQVSNYIDAQGSLSSTYTVASVVDGVEQEPSAP
                     VSVWAQAYKELTLDLPPGGVTPDNVSYSYTPNDMSAGHLDDDDRYDLVIKWEPTNAKD
                     NSHSGYTGNVYLDGYTLDGDKLWRIDLGVNIRAGAHYTQFMVYDLDGDGIAEIACKTA
                     PGTKDGLGNYLSKGPAATANHSADYRNSGGYILTGPEYLTIFDGRTGAELTTVSYHPP
                     RGSVDAWGDGYGNRVDRFLAAVAYLDGRRPSLVMARGYYTRTVLAAYDFDGTNLTQRW
                     VFDSNTSGNGGYAGQGNHNLSVADVDGDGKDEIVYGSCTIDDNGKGLYNTYLGHGDAM
                     HVSDFDPYRKGLEVFKCLESSDGGTALYDAANGDILIRHKTYNDCGRCMAGNVTDASP
                     GAEVWGGGKRFSATTRQDISGLSLNVSENFRIYWDGDLLSETLNHTTVTKPTNGSTLL
                     QASGCESNNGTKGTPGLQADLFGDWREELVFRTSNPSKIRIFTTTVPGTYRNYTLMHD
                     RQYRLAIAWQNVAYNQPPHPSYFLGEAEGIVSLPPPVMTNGRLVYKGGGVWDNTSEAW
                     TLDGQNTVYSDGRHVFLTGPQGTNDSIELSGTLQPSVLSMASPGRFYLNGENGTLSGN
                     MKLIKQGSGVFSLDGTHDFSGPTEVWDGKMIFSGSLSASPLWLNINAELAAAGHLNRG
                     LEMRYGSKLFVGGEQRAGELNITGTLRLEEKAELVFDFTGNQPGEADVIKLNGDLYMQ
                     NAAVVRILPAFAGDEQFCEGEYTLLTYTGNAQVDISKISLEGIPEIPAGLVLVNDTLK
                     LVVRSSREPATILWKGSYSNNWDLVQTKNFWYNDSSDYFVTGDTVIVNDEAGNRVLNV
                     TETVMPSALIFENTGNFVLTGKGEIAGDGTLTKRGSGKLSILNTNSFTGKVLLEEGIV
                     EVKALPNSIDGNGAIGPVSANAALFEINGGTLGVTAATTSDRALTVGSAGATLQTNAN
                     VAWNARISGGRLIKTGNHTLSLSAANTNTELLLKAGTVQLSTEQAQPGRKVIFEGGTL
                     KAFDSSGSYSSASYPLVVEEGQTGRFYVDSRCTYSNTLTGGGTLYIYVPWVRSDFEGN
                     WSNFTGTIVLMNPNAFRNQSTYGYAKAVIDLGTEGVFDDMKSQTVKIGSLKGRGRLWG
                     AG"
BASE COUNT         1919 a         1742 c         1799 g         1741 t
ORIGIN      
        1 gcctccgggt atgtacttgt ataaaattta tgccggtaaa tgctataccg gtaaaatgtt
       61 gttgccctga gaaatagtat gatacaattt caatagataa atggataatc gagcgaaaat
      121 tcaaatactt tttaactatg aaaaaacatc ttttccccct tttcgtttta ctgttgctga
      181 ctgccgggtg taagaatgca gtcaagccgc ctcaatcttt tgattaccag cccgatttgg
      241 aggctatggg tatagaggcc gagcaaattc aggccttgga ttccgtgttg caatcttttg
      301 tggatgctca aaaactgaat tgcctcgctg cttttgcggc aaaaggaggc aaggttattt
      361 attacaaggc ttttggatgg aaagacctcg aaaaccgtgt tcccgcttct gtcgatgatt
      421 attacgtgtt gttttcccaa accaaggcca ttaccaccgt ggcttttatg accttggtgg
      481 aacaaggact ggtggccatt gacgataagg tggcagatta ttttccggag atatccgacc
      541 aggtggtaac caaggtaaac gaagatggaa gctacgaaac aaggccggta gaaacaccta
      601 tgacctttgt gcatctcatg tcgcatactt cgggcctgaa cgccggacag gtgggagcca
      661 tcagacgggc agagagggcg caaatgcgaa gagccgatgc tgcttcgggg gctgaagccg
      721 gacagcgcag tttcgatgcc gatccccgct cgggaacgct cgaagacgaa atgataaaat
      781 tagccaagta tcccctggga ttcgatccgg gatcggagtg gaactaccac atcagcacca
      841 acatgctggc ctatatggtg gaacgtattt cgggcaagcc tttgcgtcaa tatgtaaagg
      901 aaaccatttt ggagccctta ggcatggatc ataccgactg gtattatccc gaggaggctc
      961 tgggacggtt cgtgctcccc ggcagtatcg tcgaaggtaa attggttgcg ggcacggata
     1021 tgtttgtgcg tggtgcagtg agccggcaac aaagctattg cgaaggcgcc atcggcttga
     1081 acggacccat cgaggactac gccaaattct gccagatgat gctaaacaag ggcgaattca
     1141 atggtcggcg aattcttaag ccggaaaccg tggagctgat gaccaccatc aaccggctgc
     1201 ccgaaaacaa ttccggaggc ggagggtttc aattcggact cggatttgaa ttgtacaatg
     1261 aaaacaaaaa acccgttccg gccgtatcca acacagccta cgcctggggc ggcatgctgg
     1321 gaacggaata catcatcgat cccgaaaacg acctgatcgt gctttattac accaatatgt
     1381 tccgtaacga gcgcctgtac ccggtttacc tggccgctgt ttatgatttg ctgttgccgt
     1441 aacaaagctg cgtattgaaa tcatacagat caggcaaccg tatcttttta atatggaggc
     1501 gtttgttatt accttttaaa aattttaaag cttaatactt ttttatttgc gtcgaaaata
     1561 ttcacaaaat aaacccccct gtgcaaaaaa gacacatcaa acttctgatt ggattccagt
     1621 tgcttgaaag ttttctgaaa caccggcttg ccttctaaat caaaaatcgc aatagattca
     1681 aaatcctttt tacaagtgat ctctaaaaaa tcacctgttg ggttgggaaa atatgattca
     1741 tctaagttgc cgttaatgcc ggaaattcca ctggcctcct ggtatatcaa ctgccatttt
     1801 tcaagacccg gatccgaagg tccaaaatcc cttcttgttt ttacttccag aatatttccc
     1861 gtggttttat caataaataa atatcggcga aaatacggac catttacatc catccattca
     1921 ttgatatggt aaacccaaga agggtagtca acgccaaatg tttcgttatt ggaattggta
     1981 atagttgaga caccgtctgc gttggataaa gaatctatcg tgtatattaa caaataatcc
     2041 attcttaatt cctcgtctgt tacgtactta tacacgattt gatcggcttg ttctcttgtc
     2101 acttgggcaa acgagatggt tgaaaatgaa aacaccttta aaagtactaa taagtacatc
     2161 tttttcataa ttgattatgt taagtgtatg attaaattcc ttattcgtgt caggcacaag
     2221 ctttttacaa aaaattcaag aagcaaccaa actcaatcca aaggaaacaa aagattaata
     2281 caaaaagcga ccaagagtgt taaaaaaaca aataacaata aattcgcact aattgtgtaa
     2341 actgattttt aaaagtttat ttctttctgt tataaatgtt ttaaatgtaa taatactaag
     2401 cccttgagta aataaaaaaa ttaaatttgc cttggtttct gttttcattt ggtattctga
     2461 atatttacga catgaatagg gaaaatgtaa ttacaaacag caaggctctg tttcttgcgg
     2521 tgatcttggt gtcaacactg ctttttggga gttgttcaaa atcgaagaag ccccgttacg
     2581 aggcttattt atttgcctat ttcgaaggta gtgccggcga ccgccaacac caggaacaat
     2641 tgcgctttgc gcttagtagc gatgccgttc actggaaggc tctcaataac aacgaaccca
     2701 tactgatttc cgaagagatt tcccgatccg gaggcatcag ggacccccat attcttcgtg
     2761 ccgaagacgg caagactttt tatctggtag ctaccgatat gtcaacaggc aggaacggct
     2821 ggaggtccaa cccgggcata gtgatgctca aatccaagga tttgattcat tgggagcaca
     2881 gcactgttca tttgtccaag gattatccgg agcattttgc cgatgcgtac tgggtgtggg
     2941 cccctcagac catctacgat ccggctgccg gcaaatacct ggtttatttt acgttgaagc
     3001 tcgaaaacga cgataaactg gatttttatt ctgcttacgc caacaaggat ttcagcggtt
     3061 tcgaagccga accttctttg atgttcagcg ccaaacacgg ggctatcgat gccgatatcg
     3121 tcttcaagga cggcacctgg catatgtttt acaaagggaa taccaaagac gagcagggca
     3181 gagaattcga aaacggcata aaacaggcaa cggcgcccag tttgcaaggc ccctggaccg
     3241 aacatttcga atacctcgat gtgtaccaca atacgcgcac caatgtggaa ggttccagcg
     3301 tgtttaaatt gaacgactcc gacacttata ttttaatgta cgatctctat tcgagcggaa
     3361 ggtacgagtt tcagcgctct caggatttgt atcgtttcac cgaaaagccc gagtcgtttg
     3421 tgaaagattt tcatcccagg cacggcagcg tcatcagcat taccgggaag gaagctgagc
     3481 gtctgaaccg aaaatggggc ggcaccgcgc tcgaataatt ctatccccaa agccgggttg
     3541 tcccggttgt atttaataac atcgtgtgta tccggtaata tcatgagcaa aaaaatcaat
     3601 ttccttgtcc ttctggcttt tttaacagcc catgtctttg cgcaacacag tctggaaaag
     3661 ctggaccgtg gtctggtagc cgtcaaaata tccaacgggg tgtttctgag ttggagaata
     3721 cccggcgaag agtggcatta taccacctac aacgtttacc gcgatggaac caagctcaac
     3781 aacgaagctt tgcaggtttc caattacatc gatgcccagg gcagtctcag cagcacctat
     3841 accgtggcgt ctgtggtgga tggagtggag caggagcctt cggctccggt ttccgtttgg
     3901 gcacaggctt acaaagaatt aacactggat ctgcctccgg gcggggttac ccccgataac
     3961 gtaagttaca gttatactcc caacgatatg agtgcggggc acctggacga cgacgacagg
     4021 tacgatttgg tgataaaatg ggagcccaca aacgctaaag acaattctca ttcgggttat
     4081 accggcaatg tttacttgga cggatacact ctggacggcg acaaactctg gcgcatcgac
     4141 ttgggtgtca acatacgggc cggagcgcat tatacccagt ttatggtgta cgacctggac
     4201 ggcgacggta ttgccgaaat tgcctgtaaa acggctcccg gcaccaaaga cggcctgggg
     4261 aactacctga gcaaggggcc tgccgccaca gcaaaccaca gtgccgatta ccgcaacagc
     4321 ggaggctaca tacttacagg ccccgaatac ctgacgattt ttgacggaag aaccggagct
     4381 gaattgacaa cggtgtcgta ccatcctccc aggggcagtg tcgatgcctg gggagacggt
     4441 tacggcaaca gggtagaccg ttttctggcc gccgtggcct atctcgacgg tcggcgtccc
     4501 agtctggtca tggccagggg gtattacacc cgcacggtgc tggcggctta cgactttgac
     4561 ggtaccaatc tgacgcaacg ctgggttttt gattcgaata cttcgggtaa cggcggctat
     4621 gccggtcagg gcaaccacaa cctgagcgtg gccgatgtgg acggcgacgg aaaagacgaa
     4681 atcgtttacg gatcctgtac catcgacgac aatggtaaag gtttgtacaa tacctacctg
     4741 gggcacggcg acgccatgca tgtgagcgat tttgatcctt accgcaaagg cctcgaagtc
     4801 tttaaatgcc tcgagtcgtc cgatggcggc acggcgctgt atgacgcggc aaacggagat
     4861 attcttatca ggcataagac ctacaacgat tgcggccgtt gtatggccgg taatgtgacc
     4921 gatgcttctc cgggagccga agtctggggc gggggcaagc gattctcggc cactacccgc
     4981 caggatatca gcggtctctc gctgaatgta tcggaaaatt tcagaatcta ctgggacggt
     5041 gatttactga gcgaaacact caaccatacc accgttacca aacccaccaa cggaagtacg
     5101 ctgttgcagg cctccggctg tgaatcgaac aacggcacca aaggcacccc cggtttgcag
     5161 gccgatttat tcggcgattg gcgcgaagaa cttgttttca ggacaagcaa tccttccaaa
     5221 atcaggattt ttactactac cgttcccggc acttaccgca attataccct gatgcacgac
     5281 cggcaatacc gtttggccat tgcctggcaa aatgtggctt acaaccagcc ccctcacccg
     5341 agttatttcc tgggcgaagc cgagggtata gtttcgcttc cgccgcctgt aatgaccaac
     5401 gggcgcttgg tgtataaggg aggcggtgta tgggacaaca cttccgaagc ctggacgctc
     5461 gacggacaaa acactgtgta cagcgacggc cggcacgtct ttttgaccgg cccccaggga
     5521 acaaacgaca gcattgaact ctccggaacc ttgcagcctt cggtgctgag tatggcttct
     5581 ccgggtcgtt tttacctgaa cggcgaaaac ggaacattat cgggcaatat gaaactgatc
     5641 aaacagggca gcggagtgtt tagtctggac ggtacccacg atttcagcgg cccgaccgaa
     5701 gtctgggacg ggaagatgat tttttcgggc agccttagcg ccagcccgct gtggttgaac
     5761 atcaacgcag aactggctgc cgcaggacac ctgaatcggg gactggaaat gcgttacggt
     5821 tcgaaattgt ttgtcggtgg cgaacagagg gcgggagagc tcaacatcac cggaactttg
     5881 cgtctcgaag aaaaagccga actggtcttt gattttacgg gcaatcagcc cggtgaggca
     5941 gatgttataa aactaaacgg cgacctctat atgcagaatg ctgccgtagt tcgtattttg
     6001 cccgcgttcg ccggggatga acaattttgc gagggagaat ataccctgct tacttacacc
     6061 ggcaacgctc aagttgatat aagtaaaata agtttggagg gcatacccga gattccggcc
     6121 ggccttgtat tggtaaacga tacgctcaaa ctcgtggtac gctcctcgcg tgagcccgca
     6181 accatcctgt ggaagggcag ctattcgaac aattgggatt tggtgcagac caaaaacttc
     6241 tggtacaacg acagctcgga ctattttgtc accggcgata ccgtcattgt caacgatgag
     6301 gccggcaacc gggttctgaa tgtgaccgaa accgtaatgc cctcggcact gattttcgaa
     6361 aacaccggca attttgtact caccggcaaa ggagagattg ccggcgacgg cacattgacc
     6421 aaaaggggca gcggcaaact gagtatctta aataccaaca gcttcacggg caaagtgttg
     6481 ctcgaagaag gcattgtgga agtgaaagct ttgcccaact ccatcgacgg caacggagcc
     6541 atcggcccgg tatcggccaa tgccgccctg ttcgaaatca acggaggaac cctcggagtg
     6601 acggctgcca ccacgtccga cagagccctg acggtaggtt cggccggagc taccctgcaa
     6661 accaacgcca acgtcgcctg gaatgcccgt atttcgggtg gtcgcctcat caaaaccggc
     6721 aaccataccc tatccttgtc ggcagccaat accaataccg aattactact caaagccggc
     6781 accgtacaac tgagtaccga acaagctcag cccggacgta aagtgatctt cgaaggaggt
     6841 acgctcaagg ctttcgacag cagcggatcc tacagttccg ccagttatcc cctggtggtc
     6901 gaagaggggc agaccggacg tttttacgtc gatagtcgct gtacctattc caataccctg
     6961 accgggggcg gaaccctcta tatttacgtg ccctgggtgc gttccgattt cgagggcaac
     7021 tggagcaatt ttaccggcac catagtgttg atgaatccca atgccttccg taatcaaagc
     7081 acctacggct atgccaaagc ggtgatcgat ctgggaaccg aaggcgtatt cgacgatatg
     7141 aagagccaga ccgtcaagat cggctcactc aaaggcaggg gcaggttatg gggagcaggt
     7201 a
//
LOCUS       DAOSGL010000085         7179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_9067_length_7179_cov_3.734980, whole genome shotgun sequence.
ACCESSION   DAOSGL010000085 DAOSGL010000000
VERSION     DAOSGL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9067_length_7179_cov_3.734980"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3118)
                     /locus_tag="PKY68_03990"
     CDS             complement(<1..3118)
                     /locus_tag="PKY68_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24229.1"
                     /translation="MIKYARYSVVLFMLFMGFSAVAAKLDGKVLSIDKSPVEGAIISV
                     VLEGRDFSAITAEDGSFSLELPSGRGYIRVVAAGFYRQEYPINQKLNLEKIVLVPLVS
                     PHYSGAAYFPNYNLSRDDKSTSTQSVEKKDFSKHFSIDQAIQDKVPGLYIHRKSGMPG
                     EGAYLNLRGIHSVVADNTPLIVINGIPLLGNQELSATVRAYSRNTLFGYSTDDIRSIT
                     LLKGADASVYGSLGSNGVLLIETEQATSDNLETRISFSGQYGLSWPGRSLPVLNVSQY
                     KNYLREIGMTRYDMITALYTDYPFLQNEENYYSYLFNNTTDWRGLVQNRAFITDNTFR
                     VEGGDEIAKYNISFGYTSEGGVLGQTASDRYHTLINSNIMVSRNVDIFTNVGLAYISS
                     SLQEQGMNAPTNAVLSSYFAMPMLSPFMKEPNGNVLDRYATYNGWNVNNNPTYPYDNV
                     SNPLAIVNTINASDNIYDANIRLGLNYRANSYLSFTGLLNIYYNYTEESIFIPGVTDK
                     AIIPQYYNAGLNTVRKGIVENLTNYYGLSSTYRRTFRDIHQLRAEAGLRYVGRKLEYD
                     LAAGYNTANDYYETLGAVTDDRDIQGDNQEWKWLNYHLHTDYIYHNLLKTSVNVALDG
                     SSVSGTDAARFGLFPSLGLTFMAANTGLLPSFVDLFNIAAEVGRTGNSRFSSNYAKNY
                     YQNSNFFNLGTITRSNVPNTALEWEKKDQLDLGLDLGLLDNKLGLQFNYFLSRSFDLL
                     IAREISSVYGSTQYYDNVGEIAGRGYELGLRLNPIVTKQVDWVIGAGIAHAGSKIKAL
                     GNNPELSIGFKEFNGDDALILMKEGEAPYQFYGYQTQGIYVSTEEAREAGLRNIYGEP
                     YKAGDVRFVDQNGDNQINARDKVLLGTAAPDFFGSFYTSLRYRRITLSAEFGYSVGNT
                     AYNAVRRNLESMDYFYNQSQAVLNRWQIEGQPAALPRAAYGDPSGNNLFSDRWLEDAS
                     YLKLRNLSLSYRFNNLLGVLRSGSIFVAAENLYTWTNYLGSDPEFSYSYSDYMQGFDY
                     AKVSLPLSV"
     gene            complement(3130..4923)
                     /locus_tag="PKY68_03995"
     CDS             complement(3130..4923)
                     /locus_tag="PKY68_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24230.1"
                     /translation="MKTTHSKIIGFYLTFFLLYSCQNAWEEHYLPEAVSSEKMEVFQG
                     DAMSFIQSSKDLSLLSGLMESYGISSGIAPDGGYTFIVYSDAVMQNRPAGNDSLLVLN
                     CVSDLALPPSRLKEGFGIYTRLGKNIWVYEKDSCLYLDEVKISRSVKVDNGYVYYVEG
                     LIPIRQSIYDYLKQLGPDYSRFVALVERFEERYFDKEKSIPIGIDPMGNTTYDTVWSV
                     RNTLMDRYTAEGLKYWDMRSEDFVSTMFIPSNDLIDQAIQAALDSIPIWLARDTTAAD
                     RTKFERWIVRACFADRRLSDEEVSPQAPAFDCVGAHQLIIDQSQDVMKYQPIEPARWN
                     PAIQTADFSSPVALSNGKAYYLNHLKIPNHVVIYRVKAKFYELWGAMTDEQKEQYFRW
                     TNWVEPLIVNDAQSEFTLTETMPTMYYHVLTAIPSEEAIADSLVCSVNYDGLLYNSKT
                     RKLTEVHLPAGEYYLRMGFKHSLRYCISIYFNDELLVEDMLLFAQGSNFHFDRGSVSD
                     MDYFGSSTIGFPEGYSWRDWIEKNEKAVAYDTDGYQVAIVNLPHSGNFTITIESNDNS
                     YLYTTVNGRSKNNVTQLMMYHWCLRPTTNNY"
     gene            complement(4934..6724)
                     /locus_tag="PKY68_04000"
     CDS             complement(4934..6724)
                     /locus_tag="PKY68_04000"
                     /inference="COORDINATES: protein motif:HMM:NF028443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5108 domain-containing protein"
                     /protein_id="HOH24231.1"
                     /translation="MKKFSILSIFILLLLSACRDPFLDEPFIKSTGEDLELSNAEFLK
                     KYEDKYSLWIQLLKHADMFNALNDASSVSTVFAPDNRAVEEFLEWRGVESVEELDYEY
                     ARYVAQVHILAQNLPEAMFIAYVETGSIPIKTIFGTYLSTSYGFMDNEVDDVFLPQVK
                     VQDSLSIYLNNQAKVTALARTTANGQVYTLGAVIRPLAETILDILAPFREYEIFIEAA
                     RLTAYDKIVSVYADTVYNLDGSFSVNNIRFTCFAVPDSVYNQVGINSAEDLAAYLGAE
                     QNYTDSLNALYRYMSYHFLGQVYSKAALFNFKREGQMLIFDTKLPGQVIVSEQEEGVS
                     KINKVAKIIRSGIQARNGVIHKIDHIMPVYEPEPIAVVWDFCNYPDVESFVNTYGASK
                     SLGDLFSSPMSNREYPVDLSEDQLDGNNGTLTSMLYKANASKTSYRTWRKVGFLKCAY
                     NSSRDKENNKYDAYMNNLLTLNLGYAGWIQLQTPTIIKGKYKVVFYYASSISFKNYYG
                     GGSLTKFNLDDYQKSIYVWKGLPAKFTDETKRNNENASGLAEDVLWEEVEFDFSGRHT
                     FKATMMDINAKTSGSYRQMWDYIEFIPISD"
     gene            complement(6726..>7179)
                     /locus_tag="PKY68_04005"
     CDS             complement(6726..>7179)
                     /locus_tag="PKY68_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24232.1"
                     /translation="FIIYRVAEVILMKAEALALRSETEQDWSEAIDLINTIRQRSNLG
                     DFEGQLGPREYSEDLTRETLLEWILYERRMELVGEGKSWYDVLRFGRRHNNAFKSKFL
                     VESVLTYNNQAGQSWLSSVLSNDDALFLPIWEEELKTNTLLVQNPYYY"
BASE COUNT         1877 a         1752 c         1655 g         1895 t
ORIGIN      
        1 taacactaag gggtaaagaa actttcgcgt aatcaaatcc ttgcatataa tccgaataag
       61 aatacgaaaa ctcaggatcg ctgcccaaat aatttgtcca ggtatacagg ttttctgctg
      121 ccacaaaaat actgccgctg cgcaaaacac caagcaaatt attgaaccgg taactcaggc
      181 tcagattgcg caatttcaga tacgaggcat cttcgagcca gcgatcggaa aacaggttgt
      241 tgcccgaagg atctccgtaa gccgctctcg gcaaagcagc gggctggcct tcgatttgcc
      301 agcggttcag cacagcctgc gattggttgt aaaaataatc catcgactcc aggttgcggc
      361 gtaccgcgtt gtaagccgta tttcccactg agtaaccgaa ttcggccgaa agcgtgatcc
      421 gtcggtaacg gagcgatgtg tagaaggaac cgaagaaatc gggagcagcc gtacccagca
      481 gcactttgtc cctggcgttg atttgattgt cgccgttttg atccacaaaa cggacatctc
      541 ccgccttgta aggttcgccg taaatattcc tgaggcctgc ttcccgggcc tcctcggtac
      601 tgacgtaaat accctgggtc tgatagccgt aaaactgata aggtgcctct ccctctttca
      661 tcaaaataag ggcatcgtcc ccgttgaact ccttgaagcc gatactcaat tcgggattat
      721 tccccaacgc tttaatttta ctgccggcat gagcaatacc ggctcctatc acccaatcta
      781 cttgtttggt aacaatggga ttcagacgca agcccaattc atagcctctg ccggcaattt
      841 ctcccacatt gtcgtaatat tgggtagaac cgtacacact ggaaatttcc cgggcaatca
      901 gcaaatcaaa agatctggaa agaaaataat tgaattgcag acccagttta ttatccaaaa
      961 gccccaaatc caggcccagg tccaattggt ctttcttctc ccattccaaa gccgtattgg
     1021 gcacgttgga acgggtaatg gtacccagat tgaaaaaatt ggaattctgg tagtaattct
     1081 tggcgtaatt cgacgaaaaa cggctgttac cggtacggcc gacttcggct gcaatattga
     1141 acaaatccac aaacgagggt aacaaacccg tgttggcggc cataaaagtc aggcccaggg
     1201 aggggaaaag cccgaaacgg gccgcatccg tccccgaaac cgagctgccg tccaaagcca
     1261 cattaacact ggttttgagc aggttgtggt aaatgtaatc ggtatgcaag tggtagttga
     1321 gccacttcca ctcctgattg tcgccctgaa tatctctgtc gtcggtgacg gctcccaagg
     1381 tctcgtaata atcgttggcc gtattgtaac ccgcagccag gtcgtattcc agttttctac
     1441 ccacgtatcg caggccggcc tcggccctga gctgatggat gtcccggaaa gtccggcgat
     1501 aagtggaact gagtccgtaa taattagtaa ggttttcgac tattcctttg cgcacggtat
     1561 tcaaaccggc gttgtagtat tgcggaatga tggccttgtc ggtgacaccg ggaataaaga
     1621 tgctctcttc ggtgtaattg taataaatat tgagcaggcc ggtaaaactc agataggaat
     1681 ttgcccggta gttgagtccc aaccttatgt tggcatcgta tatattgtcc gaagcgttta
     1741 tggtattgac tatggccaga ggattactta cgttgtcgta aggataagtg ggattgttgt
     1801 ttacattcca gccgttgtag gtggcatacc tgtccagtac attgccgttc ggttctttca
     1861 tgaagggact gagcataggc atggcaaaat aagaggaaag tacggcattg gtgggcgcat
     1921 tcatgccttg ctcctgcaaa gaactgctga tataggccag gcctacgttg gtgaaaatat
     1981 ccacgttacg gcttaccatg atattggagt tgatcagcgt atggtaacga tcggaagcag
     2041 tttgccccaa cacaccgcct tcggaggtgt agccgaaaga aatgttgtat ttggctattt
     2101 cgtcgcctcc ttccacacga aaagtattat cggtgataaa agccctgttc tgaaccagcc
     2161 ctctccagtc ggtggtgttg ttgaacaagt aggaataata attctcctca ttctgcaaaa
     2221 aaggataatc ggtgtaaaga gccgtaatca tgtcgtagcg ggtcatgccg atctccctca
     2281 ggtaattttt gtactgactg acgttcaaga ccggcaaact ccgtcccggc caactcagac
     2341 catactgacc gctgaaggaa atacgggttt ccaggttgtc ggaggtagcc tgttcggtct
     2401 cgatcagcag gaccccgttc gaacccagtg aaccatacac cgaggcatcg gctcccttta
     2461 acagggtgat actacggatg tcgtcggtgc tgtagccaaa caaagtattt ctcgaatagg
     2521 ccctgacggt ggccgaaagt tcctgattgc ccagaagggg aataccgttg atcactatca
     2581 aaggcgtatt gtcggctacc acagagtgaa tacctctcag gttgaggtag gctccttcgc
     2641 cgggcatacc gcttttacga tggatatata aaccgggcac tttgtcttgt atggcctgat
     2701 cgatggagaa atgcttactg aaatctttct tttcgaccga ctgggtactg gtgcttttgt
     2761 cgtcgcgact caagttgtaa ttcgggaaat aggccgcacc gctgtaatgg ggactgacaa
     2821 gaggaacaag cacaattttt tcaagattca gcttttgatt aatggggtat tcctgccggt
     2881 aaaaaccggc cgccactacc cttatataac ccctgcccga aggcaattcc agactaaaac
     2941 ttccatcctc agccgttata gcggaaaaat cccgtccttc caaaacaacc gagataatgg
     3001 ccccctccac gggagactta tctatactca gcaccttgcc atcgagcttt gcggccactg
     3061 ccgaaaaacc catgaacaac atgaataaaa caactgaata tctggcgtac tttatcatat
     3121 tatccctttt taatagttat tggtggttgg cctcagacac cagtgataca tcataagctg
     3181 ggtgacgttg tttttgctgc gcccgttaac agtcgtatac agataagaat tatcgttcga
     3241 ctcaatggta atggtaaaat taccgctgtg gggcaggttg actatggcca cctggtaacc
     3301 gtcggtatcg taggccacgg ccttttcgtt tttttctatc cagtctctcc aactataacc
     3361 ttcgggaaag cctatggtag aactgccgaa atagtccata tcgctgaccg aaccgcggtc
     3421 gaagtggaaa ttggaaccct gggcgaagag taacatgtct tcgaccaaca actcatcatt
     3481 gaaataaatg ctgatgcaat acctcagact gtgtttgaat cccatcctca ggtaatattc
     3541 accggccggc aaatgaacct cagtcagttt tctggtcttg gaattataca acaaaccgtc
     3601 gtaattaacg ctgcaaacca gagaatcggc aatggcttct tcagaaggaa tggccgtaag
     3661 tacatgataa tacatggtgg gcatggtctc ggtcagggta aattcagatt gagcgtcgtt
     3721 cactatcaaa ggctccaccc aattggtcca gcgaaaatac tgctcctttt gctcatcggt
     3781 catggctccc caaagttcgt aaaatttcgc cttgactctg taaatcacca catgattggg
     3841 tattttcaga tggttcaggt aataagcctt tccattgctc aaagctaccg gactactgaa
     3901 gtcggccgtt tgaatggccg gattccagcg ggcaggttct ataggttggt atttcataac
     3961 gtcctgactc tgatcgataa tgagttgatg agcccctaca caatcaaagg ccggagcctg
     4021 tggagatact tcttcgtcgc tcaggcgcct gtcggcaaaa caagccctga ctatccagcg
     4081 ttcaaatttg gtccggtcgg cagcagtagt atcgcgggcc agccatatag gaatactgtc
     4141 gagggctgcc tgaatagcct gatcgatcaa atcgttcgaa ggtataaaca tggtacttac
     4201 gaaatcttcc gaacgcatat cccagtattt caaaccttcg gctgtgtatc tgtccattaa
     4261 ggtgttgcgt acggaccaga cggtatcgta ggtggtattc cccatagggt cgatgcctat
     4321 gggtatactt ttttccttgt cgaaataacg ttcttcgaaa cgctctacca aagcaacaaa
     4381 acgggaatag tcgggcccca actgcttcag gtagtcgtat atggattggc gtatgggaat
     4441 taagccctcc acgtaatata cgtaaccatt gtccaccttg acgctgcggc ttatttttac
     4501 ttcatccaga tacaggcagc tgtctttctc gtaaacccag atgtttttac ctagacgggt
     4561 atatatacca aaaccctctt tgagtctgga cggaggcagg gccaaatcgg aaacacagtt
     4621 cagcaccagc agggaatcgt tgcctgccgg cctgttttgc atcaccgcat cggagtaaac
     4681 aataaaagta taacctccgt ccggagcaat cccggaactt ataccgtagc tttccattaa
     4741 gccgctcaac aggcttaaat ctttgctcga ttggataaag ctcattgcat caccctgaaa
     4801 cacttccatt ttttcggaac tgacagcctc cgggagataa tgctcctccc aggcattctg
     4861 acaagaatac agcagaaaga aagtcaggta aaaaccgatt attttgctat gtgtcgtttt
     4921 cataaaacag tcgttaatca ctgattggaa taaattctat gtaatcccac atttgccggt
     4981 aagaaccgct ggttttggca ttgatgtcca tcatagtggc tttgaaagta tgcctgccgg
     5041 aaaagtcgaa ctcaacttct tcccataata catcttcggc cagcccgctg gcattttcgt
     5101 tattcctttt ggtttcgtcc gtaaatttgg ccggcaaacc tttccataca tagatggatt
     5161 tttggtagtc gtccagatta aacttggtca aactgcctcc cccataatag ttcttgaacg
     5221 aaatggaact ggcgtaatag aacaccactt tgtatttacc cttgatgatg gtaggtgttt
     5281 gcaactgtat ccagccggcg taacccagat tcaaagtcag caggttgttc atgtatgcat
     5341 cgtacttgtt gttttcctta tctctggagc tgttgtaggc acactttaag aaacccacct
     5401 tacgccaggt tctataactg gtcttgctcg cattggcttt gtacaacatc gaagtcagcg
     5461 ttccattatt accgtccagc tgatcttcgc tgaggtctac cggatactct ctgttactca
     5521 tgggggaact gaacaaatcc cccagacttt tggaagctcc gtaggtattg acaaaagatt
     5581 caacgtcggg ataattgcaa aaatcccaaa caacggcgat gggttcgggt tcatacaccg
     5641 gcatgatgtg gtcaatcttg tggataactc cgttacgggc ctgaataccg ctgcggatta
     5701 ttttggccac cttgtttatt tttgaaaccc cttcctcttg ttccgatact atcacctgac
     5761 cgggcaattt ggtgtcgaaa atcaacatct gcccttcgcg cttaaaattg aacaaggctg
     5821 ctttggaata tacttgtccc agaaaatggt aggacatata ccggtacaaa gcgttcaggc
     5881 tatcggtata attttgctct gcgcccaaat aagcagccaa gtcctccgcg ctgttgatac
     5941 cgacctgatt gtacacggaa tcgggaacgg caaaacaggt aaaacgaatg ttgtttacag
     6001 aaaaactccc gtccaggttg tagacggtgt cggcataaac cgatactatt ttgtcgtagg
     6061 ctgtaagccg ggctgcttcg ataaagattt cgtattcccg gaaaggggcc aaaatatcga
     6121 gaatggtttc ggccaaagga cgaatcacgg cgcccagggt atatacctga ccgttagccg
     6181 tagttcgcgc cagagcggtc actttagctt gattattgag gtaaatcgac agagagtcct
     6241 gcactttgac ctgaggcaaa aaaacatcgt ccacttcgtt gtccataaaa ccgtaagaag
     6301 tactcaaata ggtaccaaaa atggttttaa tgggtatgga accggtttcg acgtaagcaa
     6361 taaacatggc ttcgggcagg ttttgagcca gtatatgcac ctgagccaca tagcgggcat
     6421 attcgtaatc gagttcttcg acactttcca cccctctcca ctccagaaat tcctctaccg
     6481 cccgattgtc gggagcaaac accgtcgaaa cggaactggc atcgttcaac gcattaaaca
     6541 tgtcggcgtg tttgagcaac tgtatccaca gggagtattt gtcctcgtat ttctttaaaa
     6601 actccgcatt cgacagctcc aggtcttcgc ccgtcgattt gatgaagggc tcgtccaaaa
     6661 agggatccct gcaggcagac agaagcaaaa ggataaaaat gcttagtata ctgaactttt
     6721 tcatatcaat agtaatacgg gttctgaact aataaagtat tggttttcaa ttcttcttcc
     6781 cagatgggga gaaaaagtgc gtcgtcgttg ctcaacaccg aactcagcca ggattggccg
     6841 gcctgattgt tgtaggtcaa taccgattcg accaggaatt tgctcttaaa ggcattgttg
     6901 tggcggcggc cgaagcgcag cacatcgtac caacttttgc cctctccgac caattccatg
     6961 cgacgctcgt acaaaatcca ctcgagcagg gtttcacgtg tcaggtcttc gctgtattct
     7021 ctcggcccca actgaccctc aaaatccccc agattggatc gttgcctgat ggtattgatc
     7081 agatctatgg cttcgctcca gtcttgttcg gtttcacttc tgagcgccag ggcctcggct
     7141 ttcatcaata tcacttcggc tacccggtag ataatgaaa
//
LOCUS       DAOSGL010000086         7145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_9133_length_7145_cov_4.609027, whole genome shotgun sequence.
ACCESSION   DAOSGL010000086 DAOSGL010000000
VERSION     DAOSGL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9133_length_7145_cov_4.609027"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PKY68_04010"
     CDS             <1..959
                     /locus_tag="PKY68_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24233.1"
                     /translation="PTNNINPNNYFFLSGNPDDQKMSNFMLRSKVRITPMIEWEAIVI
                     PIYRPSVYRYELFDLGASFVDPVLPEQKAGNGSLATRFNFELPGVGFSLSWFQGRDPF
                     YGINLNSVDLSSGTPQVSYRPAFYRRTTFGLDFAVPLSSWIIRGEGAYNFTENEHARM
                     YIPNCDLSYVMGVEYNFGSVTAILQYVGKHVLDFVDLNEPVLSDPMNQLALYQYANER
                     VNYESFLINRKIFKQQFETNHALALTLSGDFVYSTLNAEMTGFYDFSTEEFLLRPKLV
                     WNITDALALSAGYSYMKGPDKSMFSYAGPVLNGAFLEMKFSF"
     gene            1062..3251
                     /locus_tag="PKY68_04015"
     CDS             1062..3251
                     /locus_tag="PKY68_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOH24234.1"
                     /translation="MTKTTVNPDLESYLPDSMQSKQNSRKIEEIFGAEEPMLIVLESD
                     DILNKSSLERIEQLDRAFSMQPAFKRVFSLFGVQDIHSEEGVMLVDPLIDAIPQSPAE
                     TDILRERIKSNDLAYGLVVSDDFRYSLILLSSDKSLEDAELLNIIKQTLEKYPGDEKV
                     MISGQPLLREEASIKIGRDLMILLPLGLLVMFVFLWVSLREARGVFLPISVVIFSVVV
                     SMALIPLFGWELSIIGVLIPIMMLAIANNYGVYFIARYQDLNAQDPRLTMPQIVEKSF
                     SYLFKPVLFYGLTTMVGIMGLTTHLLIPARQMGVVTGLGIAFALVASLLFVPAVLSLM
                     KKGKPHHDLSGKPNGPISAFLYRVAVVVVRHPKRVLIGFSVFFALAVTGLLFFKLSPD
                     TNNVLPEKHTFNRAIAAIDKHMGGSKMITLMFEADAKDPALLKELERYDLELSALPQV
                     GSVASLATVVKEISKALNDPTEPGYDQIPSSREAVAQYLELYTMSADPEDLEQFVDFD
                     FEHTLLTIQYQAASIKEIDEVLHKIEELSESSEFTPLPGGYSLVEKELNESIMTGQNY
                     SLLFALGAIIILVALIFRSLVAGLIGGIPLVFAVFSIFGLMGWTGIELTIVTALLSSI
                     SIGLGVDFTIQIFWRIQWELNRGKTHSAAIRMALKTIGRGIVINAFAVMLGFSVLFFS
                     AFPLVRSFGFLIILSLMLCLISALILIPAICMIWKPKFLSKKNTINA"
     gene            3274..4038
                     /locus_tag="PKY68_04020"
     CDS             3274..4038
                     /locus_tag="PKY68_04020"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOH24235.1"
                     /translation="MRSFIFISILLLAPLASAQDAREIIRKAGDVIEPESMEMISTLK
                     IISASGQERSRTISMATKKFGEATKSMLRFTEPADVRGTTLLIYDYEDKDDDMWIYMP
                     ALRKTRRIVSSEKGKNFMGSEFTNADMSKPVLDDFNYKSLGEAVVDGKTCHKIESSCK
                     TDALAAQYGFSKRISYIGKDDFFAYKLEYYDTNGELHRINILKDYRELGKGKYFAFYM
                     EMQNVKNNRRSVMSVDKFQMGSAMPEAQFSPTMIEQ"
     gene            complement(4086..4907)
                     /locus_tag="PKY68_04025"
     CDS             complement(4086..4907)
                     /locus_tag="PKY68_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24236.1"
                     /translation="MKQLLYSLVLLLGITACNCDCREQLPKPAISTPPAELQLDEFYK
                     KYINVNGIHLISSHRVPDSALYAAYRTLDAMTSMLPPEVLKAMTDINTRVGVMARYEG
                     TTDIPEHAHLANDKRLNWDVRARGLGGTLRSPLTTCAEENILCYQIDKYHAEDILVHE
                     FAHSIHLIGIISIDPGFNDRLQQALDAAIAEGKWNNTYAQTNISEYWAEGVQSWFNVN
                     AEVEYPDGKHNHVNTRSDLKKYDPRLYAILAEFFPESKEQISCHKHINLYRREKR"
     gene            complement(4919..7129)
                     /locus_tag="PKY68_04030"
     CDS             complement(4919..7129)
                     /locus_tag="PKY68_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOH24237.1"
                     /translation="MLIAQGKVEDYKRAYSIRELYSGSKVYYSDVNPTWIGDSDNFWY
                     IRNTPEGREYVLVDAGDKNSKPLFDHQTLAGKLGQATGEQVEVLKLQLERLRVNDELS
                     QLSFGFKGKNWVYYLAQDSLQGETVSMERPGRTGSRTHWMVTDEERDRGAVNSPDNKY
                     QAYIKDHNVYVRELPRGKEIALSIDGCVGNYYSSYIQWSPDSRMLATTKIRPVEKRYI
                     HMVESSPSDQLQPKLRTLEYTKPGDELRFRVPCIFKVETQEALIPSTELFNNQYQLSG
                     LRWNKDNSGITFEYNERAHKVYRVLEMSAQNGLVRSLIEETAETFVNYSRRFRHDLRN
                     DEEIIWMSERDNWNHLYLYDRKSGSVKNQITKGEWYVRDIIRVDEDARRIYFTANGVP
                     KKGDPYLVRYYYINMDGSGLTCLTPDEGHHRATFSRDMKYLVDVYSRVDLAPIAQLRD
                     AESGKLIMPLEKADISELEKTGWKAPEVFVAKGRDGKTDIWGIIIRPSNFDPNKKYPI
                     IEYIYSGPGSHYVPKSFAPYYSDMTNLAELGFIVVKMDGMGTSFRSKAFEEICYQNLK
                     DAGFPDRILWIKAAAKKYPYMDVDKVGIFGASAGGQEAMAAVLFHPEMYKAAYSSCGC
                     HDNRMDKIWWNEQWMSYPIGKQYLESSNVVNAHLLTRPLMLVVGELDDNVDPASTMQV
                     VDALIKANKDFELVVLPGVGHTMGGSYGEHKRFDFFVRELLNVVPPKWNEIKTD"
BASE COUNT         1685 a         1628 c         1818 g         2014 t
ORIGIN      
        1 atcctaccaa caatatcaat cccaacaatt acttcttctt gtcgggtaat ccggacgatc
       61 agaagatgtc taattttatg ctcagaagca aggtaagaat cacccccatg atagaatggg
      121 aggccattgt tattcccatt taccggccgt cggtgtaccg ttacgaatta ttcgatttgg
      181 gagcttcttt tgtcgatccg gtgttgcccg agcaaaaagc aggcaacggc agtctggcca
      241 cccgctttaa ttttgaattg cccggggtcg gtttttcgct ctcctggttt cagggccgcg
      301 atccctttta cggcattaac ctgaacagtg tagatttgag ctcaggcact ccgcaagtca
      361 gctacaggcc ggctttttat cgcagaacca cctttgggtt ggattttgcc gtgcctttgt
      421 cttcctggat aatcagggga gaaggagcct ataattttac cgaaaacgag catgcaagaa
      481 tgtatattcc caattgtgac ctgagctatg tgatgggggt ggagtataat ttcggttccg
      541 ttacggccat tttgcaatac gtgggtaaac acgttctcga ttttgtcgat ttgaacgaac
      601 cggtgctgag cgaccctatg aatcagcttg ccctgtatca gtacgccaat gagagggtca
      661 attacgaatc ttttcttata aatcgcaaaa tatttaaaca acagttcgaa acgaatcatg
      721 ccctggcttt gactttgtcg ggagattttg tctatagcac tctgaatgct gaaatgacgg
      781 gcttttacga tttttctacc gaagaattcc tgctcaggcc caaattggtc tggaatatta
      841 ccgatgccct ggcactcagt gccggctatt cgtatatgaa aggccccgat aaatcaatgt
      901 tcagctatgc aggtcctgtt ttgaacggag cttttctgga gatgaagttc agtttttgat
      961 tatcaagata gacgacgatg agatctaaac attttatttt acgctaccgc tggtggatta
     1021 tagctgcggt tgtattaacg gtagcgttat gtatttggcc attgaccaag acaactgtca
     1081 atcccgatct ggaaagctat ttgccggatt cgatgcaatc taaacaaaac agccggaaga
     1141 tagaagaaat ttttggtgcg gaagagccga tgctgatagt attagagagc gacgatattt
     1201 taaataaaag cagcctggag cgcatagagc agctcgacag ggctttttcg atgcagcctg
     1261 cctttaaaag ggttttctct ttgttcgggg ttcaggatat tcactcggaa gagggcgtga
     1321 tgctcgtaga tccgctgata gatgccattc ctcaaagccc tgccgaaacg gacattctca
     1381 gagagaggat aaaaagcaac gatttggctt acggactggt ggtttcggac gatttccgtt
     1441 acagcctgat tttgctgagc tccgacaaat cgctcgaaga cgcagaattg ttgaatatca
     1501 tcaaacaaac cctggaaaaa tacccgggcg acgaaaaggt gatgatttcc ggtcagcctt
     1561 tgttgcgcga agaagccagt atcaagatcg ggcgggacct gatgatttta cttcccctgg
     1621 gtttgcttgt gatgtttgtg tttttgtggg tgtctttacg agaggccaga ggggtgtttt
     1681 tgccgatttc ggtggtgatt ttttccgtcg tggtaagcat ggcgctgatt ccccttttcg
     1741 gctgggaatt gagtataatc ggtgtactca ttcctataat gatgctggct atagctaata
     1801 attacggagt ctattttatt gcccggtatc aggatttaaa tgcacaggat ccacgcctta
     1861 ccatgcctca gatagtagaa aagtcttttt catatctgtt caaaccggta ctgttttacg
     1921 gattgaccac catggtggga attatggggt tgacaacgca tttgttgata ccggcccggc
     1981 aaatgggcgt ggttacaggc ttgggaatag cctttgcttt ggtggccagt ttgttgtttg
     2041 ttccggctgt gttgtcgttg atgaagaaag gcaaaccgca ccatgattta agcggcaaac
     2101 ccaacgggcc catctctgct tttttgtatc gggtggccgt tgtggtggtt cgtcacccca
     2161 agagagtcct tattggtttt tcggtgttct ttgctctggc cgtcaccggt ttgctgtttt
     2221 ttaaactcag tcccgatacc aataatgtgt tgcccgaaaa acacactttc aacagagcca
     2281 tcgcagccat cgacaagcac atgggaggca gtaaaatgat tacgctcatg tttgaggccg
     2341 atgccaaaga tcctgccttg cttaaagagc tggagcgtta cgatctggaa ctcagcgcct
     2401 tgcctcaggt gggtagcgtt gcttcgctcg ctactgtcgt caaagaaatc agcaaggcat
     2461 tgaacgaccc cacggaaccg ggttacgatc aaattccttc gagccgggag gccgttgcgc
     2521 agtacctgga attgtacacc atgagcgccg atcccgaaga tttggagcaa ttcgtcgatt
     2581 tcgactttga acatacttta ctgacgattc agtaccaggc tgcctcaatc aaggagatag
     2641 atgaggtctt gcataaaata gaagagctca gcgagagcag tgaattcaca ccattgcctg
     2701 gaggttacag tctggtggaa aaagaactga atgaatccat catgacaggt caaaactatt
     2761 cgcttctgtt tgccctggga gctattataa tcttagtagc gctgattttc agaagtctgg
     2821 tggccggcct gattggcggt attcccttgg tttttgccgt cttcagtatt tttgggctga
     2881 tgggttggac aggtatagaa cttaccatag tcacagcgtt gctttcgtcc atctccatcg
     2941 gtttgggtgt ggactttacc atacaaatat tttggcgcat acaatgggaa ctcaacagag
     3001 gtaagactca ttccgcagct atcagaatgg ccctaaagac cataggcaga ggtatagtga
     3061 tcaatgcctt tgccgtgatg ctgggctttt ccgttttgtt tttctcggct ttccccctgg
     3121 ttcgttcctt cggatttctg atcatacttt cgttgatgct ctgtctgata agcgccttga
     3181 ttttgatacc ggctatttgc atgatctgga agccgaaatt cctaagtaaa aagaatacta
     3241 taaatgccta attttcaatt taactatcct attatgagat cctttatttt tatttcaatc
     3301 ttattgctgg caccattagc ttctgcacaa gatgcccgcg aaataatacg caaagccggc
     3361 gatgtgatag agccggaatc catggaaatg atatcgacac ttaaaatcat cagtgccagc
     3421 ggccaggagc gctcccggac catttcgatg gctaccaaaa aatttggaga agctaccaag
     3481 agtatgcttc ggtttacaga gccggccgac gtaagaggca ccaccctcct gatctacgat
     3541 tacgaagaca aggacgacga catgtggata tatatgcccg ccctgcgcaa aacccgccgt
     3601 atagtgagca gcgaaaaggg caaaaatttt atgggcagtg aattcaccaa tgccgatatg
     3661 agcaagcccg tcctggacga ttttaattac aaatctttag gcgaagccgt ggtcgacggt
     3721 aaaacctgtc ataaaataga atccagttgc aaaaccgatg ccctcgccgc tcagtacggc
     3781 ttcagcaaga ggatttccta tatcggtaag gacgactttt ttgcctataa actggaatat
     3841 tacgatacaa acggtgaact tcaccggatc aatatcctca aagattaccg ggaattgggc
     3901 aagggcaaat actttgcctt ttacatggaa atgcaaaacg taaagaacaa tcgccgctcg
     3961 gtgatgagcg tggataagtt tcaaatgggc tctgctatgc ctgaagcaca gttttcacca
     4021 acaatgatcg aacaataagc cggtgtctta gaagcgccca actgcggcgc tttcaatatt
     4081 cgggctcaac gtttttccct gcggtaaagg ttgatgtgct tgtggcagga tatttgttcc
     4141 ttgctttcgg ggaaaaactc agctaaaata gcgtacaggc gcggatcgta ttttttgagg
     4201 tccgatcttg tgttgacgtg gttgtgtttg ccgtcgggat attccacttc ggcgttgaca
     4261 ttgaaccagc tttgtacacc ctcggcccag tattctgaga tattggtctg agcgtaggtg
     4321 ttgttccact taccctcggc aatggcggca tccagcgctt gttgcaatct gtcgttgaag
     4381 cccgggtcta tgcttataat tccgatcaga tggatggaat gggcaaattc gtgaaccagg
     4441 atgtcttcgg cgtggtattt atcaatttga tagcagagta tgttttcttc ggcacaggtg
     4501 gtcagcgggc ttcttaacgt accgcccagg ccccgggccc tgacatccca gttgagtcgt
     4561 ttgtcgttgg ccagatgtgc gtgttcgggt atatcggtgg tgccttcgta acgggccata
     4621 acacccaccc gcgtattgat gtcggtcatt gctttgagta cttccggagg caacatgctc
     4681 gtcatggcat ccagagtgcg gtaagccgca tagagcgctg agtcgggcac ccggtgcgaa
     4741 gaaatcaggt ggataccgtt tacgttgatg tattttttgt aaaattcatc cagttgcaat
     4801 tctgccggag gggtactgat ggcaggtttg gggagttgtt cccggcagtc gcagttgcag
     4861 gcagttatgc ccagcagcaa aacaaggctg tacaatagtt gtttcatagg tatatttttt
     4921 agtcggtttt gatttcattc catttgggag gaaccacatt gagcagttcc ctgacaaaga
     4981 agtcgaagcg tttatgctcg ccgtaagagc cacccatggt gtgacctacg ccgggcaata
     5041 cgaccagctc aaaatctttg ttggctttta tcagggcgtc caccacctgc atggtcgagg
     5101 ccggatcgac gttgtcgtcg agttcaccta ctaccaacat cagcggacgg gtcaataaat
     5161 gagcgtttac tacgttggag ctctccaggt attgttttcc gatgggataa ctcatccact
     5221 gctcgttcca ccagattttg tccatacgat tgtcgtgaca gccgcaagac gaataagcgg
     5281 ctttgtacat ctcaggatga aagagtacgg ctgccatagc ttcctggcct ccggccgagg
     5341 ctccgaaaat acccacttta tcaacgtcca tatagggata ctttttggcg gcggctttga
     5401 tccaaagtat gcgatcgggg aagccggcgt ctttcaggtt ttgataacag atttcttcga
     5461 aggccttgga gcggaaagag gtgcccatgc cgtccatttt tactacaata aagcccagct
     5521 cggccaggtt ggtcatatcg gagtagtagg gagcaaaaga tttcggcaca tagtgactgc
     5581 cggggccgga ataaatgtat tctatgatgg ggtatttctt gttgggatcg aagttggaag
     5641 gacggatgat gatgccccag atgtcggttt taccgtctcg gcctttggca acaaagacct
     5701 cgggcgcttt ccaaccggtt ttttccagtt cgctgatgtc ggccttttcc aggggcatga
     5761 ttaatttgcc gctttcggcg tcgcgcagtt gagctatcgg ggctaaatca acgcgggaat
     5821 acacatccac gaggtatttc atatcccggg aaaaagtagc ccggtgatgg ccttcgtcgg
     5881 gagtaagaca ggtcagtccg ctgccgtcca tgttgatgta gtaatacctg accaggtaag
     5941 gatctccttt tttgggtacg ccgttggcgg tgaaatagat gcgccgggca tcttcgtcca
     6001 ctctgatgat gtcgcgcaca taccattccc ccttggtgat ttgattttta acgctaccgc
     6061 ttttgcggtc gtagaggtag aggtggttcc aattgtcgcg ttcgctcatc caaatgattt
     6121 cttcgtcgtt tctcagatcg tggcggaaac ggcgcgaata attgacgaag gtttcggctg
     6181 tttcttcgat caggctgcgt acaaggccat tttgagcaga catttccagt acacggtaga
     6241 ctttgtgcgc ccgttcgttg tattcgaagg taatgccgct gttgtctttg ttccagcgca
     6301 aacccgatag ttgatattga ttgttgaaca attccgtcga gggaataagg gcttcttggg
     6361 tttcgacttt gaagatgcag ggcacccgga aacgcagctc atcaccgggc ttggtatatt
     6421 ccaaagtccg gagtttgggt tgcaactgat cggagggcga cgattccacc atgtgaatgt
     6481 aacgtttctc caccggcctg attttggtgg tggccagcat gcgggaatcg ggcgaccatt
     6541 ggatgtagga ggaataatag tttccgacac agccgtcgat gctcagggca atttctttgc
     6601 ctctgggtaa ttccctcaca tatacgttgt ggtcttttat ataagcctgg tacttgttgt
     6661 ccggggagtt taccgccccc cggtcgcgct cttcgtcggt taccatccag tgggtgcggc
     6721 tgccggtccg gccgggacgt tccatactga cggtttctcc ctgcaagctg tcctgagcta
     6781 aatagtaaac ccagttcttt cccttgaagc cgaacgatag ttgacttaat tcgtcgttta
     6841 ccctcagcct ttcgagctga agtttcagga cctcgacttg ctcgcccgtg gcctggccga
     6901 gtttgcctgc caaggtttgg tgatcaaaaa gcggcttgct gtttttgtct cccgcatcga
     6961 ccaatacgta ttcccgtcct tcgggagtgt tgcggatata ccagaaattg tcgctgtctc
     7021 ctatccaggt aggatttacg tcggaataat aaactttact gcccgagtac aactccctga
     7081 tggagtaagc tcgcttgtag tcttcgactt ttccctgagc tatgagcaga gtgaaaggcc
     7141 ataag
//
LOCUS       DAOSGL010000087         7118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_9184_length_7118_cov_3.833782, whole genome shotgun sequence.
ACCESSION   DAOSGL010000087 DAOSGL010000000
VERSION     DAOSGL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9184_length_7118_cov_3.833782"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1835
                     /gene="ftsH"
                     /locus_tag="PKY68_04035"
     CDS             48..1835
                     /gene="ftsH"
                     /locus_tag="PKY68_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOH24238.1"
                     /translation="MLILNSLVFPNLFKPKIIPTTYSAFINKVDSGLIKQVNIDGNKI
                     YFATPGDTIDRKGDTIDLIFQTGKMNDANLVDRLLVADSPNENGKIEFTETIPRENSP
                     ILSFLLYWVLPGAIFFFIWRNAMKSMGGRMGGNYMSFGKNKEKIYAEDEIKTTFADVA
                     GQDEAKESLNEIIDFLHNPKKYNEMGAALPKGVLLVGPPGTGKTLIARAVAGEAKVPF
                     FALSGSEFVQMFVGMGAAKVRDLFSQANQKAPCIIFIDEIDAIGKRRDGMTSNDEREQ
                     TLNQLLTEMDGFDGRKGVVILAATNRPESLDKALLRPGRFDRRVQMELPDLEGRKAIL
                     QVHLKKVKHEEIDLDLIARATSGASGAELANIVNEAALRAVRNESSSVTTADLEESIE
                     TVIAGAQRKGKVISDEEKRIIAYHEIGHAMVAAMQSHSAPVHKITIIPRTSGALGYTM
                     QIDEGERNLYSKEDLENRIATFTGGRAAEEVIFNRITTGASNDIEQATKIARAMITRY
                     GMSEEFDMVALETINNPYLSSDTSLSCSPETAARIDDVVLSIVKNAHEKAKKIIRENE
                     AKMHELSEYLIKKETITGEEFMKILEAKK"
     gene            1858..2424
                     /locus_tag="PKY68_04040"
     CDS             1858..2424
                     /locus_tag="PKY68_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOH24239.1"
                     /translation="MRQSFKKIEKAIKRWYLMLVVGVIFIVLGIWTLATPVDAYIALS
                     FVFAMGFLVSGITEMVFALSNKHQNWGWSLVLGILSTVVGVLLLINPAASMVTLPLYV
                     GFTLLFRSISGMTAAYDMKQYGILDWGTLMVTAVLGLILSFVLLWNPGFASINVVIWT
                     AVAFIVLGGYSIYFSFELRKLNKLMKEE"
     gene            2559..3746
                     /gene="kbl"
                     /locus_tag="PKY68_04045"
     CDS             2559..3746
                     /gene="kbl"
                     /locus_tag="PKY68_04045"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOH24240.1"
                     /translation="MYEKFKEFLSNELESIRASGLYKDERVIHSPQAANIKLADNREV
                     LNFCANNYLGLSNHPLVREAAARVLEERGYGMSSVRFICGTQDLHKELEKEISCFFGT
                     EDSILYASCFDANGGLFEPLLSEQDAIISDALNHASIIDGVRLCKAKRYRYANADVED
                     LEKQLKEAQSQRFRLIVSDGVFSMDGNVAPLDKIYALAQQYDAMLMLDESHSAGVVGQ
                     TGRGITELFGLKGRPEIITGTLGKAFGGAIGGFTTGRREIIQMLRQRSRPYLFSNSIP
                     PMVAAAGIIVFRMLQNTNELQDKLHRNTAYFVQKMSALGFDLKPTESAICAVMLYDAR
                     LSQEFADRMLDEGIYVIGFSYPVVPREQARIRVQLSAEHETEHLDRCIAAFAKVGKAL
                     DVI"
     gene            3808..4833
                     /gene="tdh"
                     /locus_tag="PKY68_04050"
     CDS             3808..4833
                     /gene="tdh"
                     /locus_tag="PKY68_04050"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOH24241.1"
                     /translation="MKALVKLRPEKGIWMCERPIPTPGINEVLVKINKTSICGTDLHI
                     YKWDDWSQRTIKTPMIIGHEYAGEIVEMGKGVENLKIGDRVTGEGHIACGHCRNCRRG
                     KLHVCENVVGIGVNRDGAFAEYLAVPASNIVRLDPRISDEMASIMDPFGNATHAALSF
                     PLTGEDVLITGAGLIGSMATAVCRFAGARYIVVSDVIEYRLELARKMGATLTVNPAKG
                     ESIEDAIKSLRMRGFDIGLEMSGSPQAFESMIENMYNGSKIALLGILPDSTQVNWNQI
                     IFKALTLKGIYGREMWETWYQMEQMLITGIDLSPVITHRFDVDDFQKAFDVMESAACG
                     KVILNWA"
     gene            complement(4862..5674)
                     /locus_tag="PKY68_04055"
     CDS             complement(4862..5674)
                     /locus_tag="PKY68_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH24242.1"
                     /translation="MKQQYFKDKVVVITGASSGIGLACARLFAAQGAKLALAARSLDK
                     LQVLQKELPTTVLVVKTDVSKEEDCRKLIEQTIATYGGIDILVNNAGISMRAVFKDLD
                     LKVLHQLMDVNFWGCVYCTKYALPSLLERKGSVVGVTSIAGFKGLPARTGYSASKFAM
                     NGFLDTLRTEHLYDDLHVMIFAPGFTASNVRFSALTADGSPQGETPRKEEKMMSAETV
                     ARHMLRGLRKRKDQMILTPLGKAAVLVNKFFPKLVSRMEYKMMKAEPDSPLS"
     gene            complement(5693..>7118)
                     /locus_tag="PKY68_04060"
     CDS             complement(5693..>7118)
                     /locus_tag="PKY68_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOH24243.1"
                     /translation="LVEAGRPVLVGTTSVEISELLSRTLKLRKIKHDVLNAKLHQREA
                     EIVANAGQKGVVTIATNMAGRGTDIKLTEEVRKAGGLAIIGTERHESRRVDRQLRGRS
                     GRQGDPGSSVFYVSLEDDLMRLFGSERISGIMDRMGLEEGELIENPMISKAIERAQKK
                     VEENNFGIRKRLLEYDDVMNNQREVIYTRRRHALMGERIGMHLANTIYDTIKSLVEEA
                     YDQRDFEGLKMDILKYLAMEVPMDEEHFYDQRAENSVDEIYEAALNLYKRKTEKMASI
                     VNPVIQQVYEKQAAQYENILIPITDGKRTYNIPCNLKAAAESQSKEVVKTFEKAILLH
                     TIDEAWKENLRELDELRQSVQNASYEQKDPLLIYKLESYNLFKTMIDNINKKSISILM
                     RGQIPIREPESVRKAETQKRHDYSKYRAQRDSLDGGSPARRAGSDREQRQLEPVRVEK
                     KVGRNDPCPCGSGKKYKNCHGRGL"
BASE COUNT         1807 a         1593 c         1803 g         1915 t
ORIGIN      
        1 aaagacccaa taacaattgg aaaagttttt tgtggacact tgccttattg ctgatactta
       61 attctttggt ttttccaaat ctgttcaaac ccaaaattat tccgacaacc tacagcgctt
      121 ttatcaataa agtcgattcc ggattaatca agcaggtaaa cattgatgga aacaaaattt
      181 attttgctac acccggcgac accatcgacc gtaagggcga caccatcgac ttgatttttc
      241 aaacaggaaa aatgaacgat gccaatttgg ttgaccgttt attagtggca gacagcccca
      301 acgaaaacgg aaaaatagaa tttaccgaaa ccattccccg cgaaaattcc cccatattga
      361 gtttcttgct ttactgggta ttgccgggag cgattttctt ctttatttgg agaaatgcca
      421 tgaagtcgat gggtggaaga atgggtggaa attatatgag tttcgggaaa aacaaagaga
      481 aaatttacgc cgaagacgag ataaaaacca cctttgccga tgtggcaggg caagacgaag
      541 ccaaagaatc tttaaacgaa ataatcgatt ttctgcataa ccccaagaaa tataatgaaa
      601 tgggtgctgc cttgcccaaa ggtgttttat tggttggccc tcctggaaca ggtaaaacgt
      661 tgattgcaag agctgttgca ggcgaagcta aagtgccgtt ctttgccctt tccggatctg
      721 aatttgttca aatgtttgta ggaatgggtg ctgccaaagt gcgcgatttg tttagtcaag
      781 cgaaccaaaa agcaccctgt atcatcttta tcgatgagat tgatgccatc ggcaaaaggc
      841 gcgacggaat gacttccaac gatgagcgtg aacaaacatt gaatcaattg ctcacggaaa
      901 tggacggttt cgacggacga aaaggagtgg ttattctggc agcgaccaat cgccccgaaa
      961 gtctcgacaa agcattgctc cgtcccggca ggttcgaccg gcgggttcaa atggaacttc
     1021 ccgatttgga aggacgtaaa gccatcttgc aggtgcatct gaaaaaagta aagcacgaag
     1081 aaatagatct ggatttgatt gcacgcgcca cctcgggtgc atcgggtgcc gaactggcca
     1141 atattgtgaa tgaagccgct ctgagagccg taagaaacga aagcagcagc gtgacaaccg
     1201 ccgacttgga agaaagcata gaaaccgtaa tcgccggagc acagaggaaa ggaaaagtaa
     1261 tttccgacga agaaaaacgc attattgctt atcacgaaat cggacacgct atggttgccg
     1321 ctatgcagtc gcattccgct ccggtgcata aaattaccat tatcccacgt acatcggggg
     1381 cgctcggtta taccatgcaa attgacgaag gtgaacgtaa tttatatagt aaagaagatt
     1441 tggaaaacag aatagccaca tttaccggtg gacgtgccgc cgaagaggtt atttttaacc
     1501 gaattaccac aggtgcatcc aacgacattg aacaagccac aaaaattgcc cgtgccatga
     1561 ttacccgcta cggtatgagc gaggaatttg atatggtggc gttggaaaca ataaacaatc
     1621 cctatttgag cagcgacaca tctttaagtt gttctcctga aacggcagcc cgtatcgacg
     1681 atgtagtgct ttccatagtg aaaaatgcgc acgaaaaagc gaaaaaaatc attcgcgaaa
     1741 acgaagccaa aatgcacgag ttatcggaat atctcattaa aaaagaaacc attacaggag
     1801 aagagtttat gaaaatattg gaagctaaaa aataaataac gtataaaatt caacatcatg
     1861 agacagtcat ttaagaaaat agaaaaagcc attaaacgat ggtatttgat gctcgttgta
     1921 ggagtgatat ttatagtttt aggtatttgg acactagcca cgccggtaga tgcctacata
     1981 gctttgtctt ttgtgtttgc tatgggattt ctagttagcg gaattaccga aatggttttt
     2041 gccttaagca acaaacatca aaattggggt tggtcgctgg tattggggat attgagtact
     2101 gttgtggggg ttcttctact gatcaatcct gctgcgtcta tggtaacttt accactttac
     2161 gttggattca ccttgctttt ccgctccata tcgggcatga ccgcggctta cgacatgaag
     2221 caatacggta ttttagattg gggtacctta atggtaaccg ccgtattggg cttgatttta
     2281 tcttttgttt tgctttggaa tccggggttc gcaagtatca acgtggttat ttggacggcg
     2341 gtagcattca tcgtcttagg aggctattcc atttatttct cctttgaatt gcgaaagctg
     2401 aataaattga tgaaagagga ataaaatttc aataatcttt cataggtgtt cgtgcgggcg
     2461 cgcttttttt taagcgtgcc cgctgttgtg tttgtttgtc aagaattatg aataaatttg
     2521 tggcaaatag gggtagaatt aaactaaaac acaatactat gtacgaaaaa tttaaagaat
     2581 ttctcagtaa tgaactcgaa agcattcgtg cttcgggttt gtataaggac gaaagggtga
     2641 ttcactctcc tcaggcggcc aacataaaat tagccgataa ccgtgaggtg ttgaattttt
     2701 gcgccaataa ttacctggga ttgtctaacc atcctctggt gagggaagcg gcggcccggg
     2761 tgctcgaaga gagaggatac ggcatgtcgt cggtgcgttt tatttgcggc acccaggatt
     2821 tgcacaaaga attggaaaag gaaatctcct gcttcttcgg tacggaagat agtattctct
     2881 acgcttcttg ttttgatgcc aacggaggtt tgtttgaacc tttgctgagc gagcaggacg
     2941 ccattatttc cgatgcgctc aaccatgctt ccattatcga cggggtcagg ctttgcaagg
     3001 ccaaacgcta ccgttacgcc aatgccgatg tggaggattt ggaaaagcaa ttgaaggaag
     3061 cacagtctca gcgttttagg ctgatcgtaa gcgatggagt cttttcgatg gacggcaacg
     3121 tggctccatt ggataaaatc tacgccctag ctcagcaata cgatgctatg ctgatgctgg
     3181 atgaatcgca ttcggcagga gtggtagggc aaacgggcag gggaataacc gaattgtttg
     3241 ggctcaaagg ccggcctgaa atcattacag gcaccttagg taaagctttt ggcggagcca
     3301 taggcggttt tactaccggg cgccgcgaaa ttattcagat gttgcgtcag cgatccagac
     3361 cttacctgtt ctccaattcc attccgccca tggtggccgc tgccggtatc atagtgttca
     3421 gaatgctgca aaacaccaac gaactccagg ataaactgca tcgcaacacg gcctattttg
     3481 tgcagaaaat gtctgctttg ggcttcgatt taaaacccac ggaatcggcc atatgcgctg
     3541 tgatgcttta cgatgccagg ctttcgcagg aatttgccga ccgcatgctc gacgaaggca
     3601 tttacgtgat aggcttctct tatccggtgg tacccagaga gcaggcccgt attcgtgttc
     3661 agctctcggc cgaacatgaa accgaacact tggatcgttg cattgctgcc tttgctaaag
     3721 tgggcaaggc tctggatgtg atatgataat ttcaactaga gcgttgaact ataagagctt
     3781 attggtttta tttaacagat ttccaagatg aaagctttgg taaaactgcg ccccgaaaaa
     3841 ggcatctgga tgtgtgagag acccattccc acgcccggta tcaacgaggt gctggtcaaa
     3901 attaacaaaa cctccatttg cggcactgat ctgcatatct acaaatggga cgactggtcg
     3961 cagcgtacca tcaagacacc catgatcatc ggacacgagt acgcaggaga aattgtggaa
     4021 atgggtaagg gtgtggaaaa tttaaaaatc ggcgaccggg tgaccggcga agggcacatc
     4081 gcctgcggac attgccgcaa ttgccgtcgg ggcaagctgc atgtctgcga aaatgtggtc
     4141 ggtattggag tgaatcgcga cggagccttt gccgaatacc tggcggttcc ggcttcgaac
     4201 atcgtaagac tcgaccctcg catcagcgac gagatggcat cgatcatgga tcctttcggt
     4261 aacgcaacgc atgcggcttt gtcgtttccc ctgacaggcg aagacgtgct tatcaccgga
     4321 gccggcctga tcggctccat ggcaacggct gtttgccgct ttgccggagc ccgttacata
     4381 gtggtgagcg atgttatcga ataccgtttg gagctggctc ggaaaatggg cgccaccctc
     4441 acagtgaatc ctgccaaagg cgagagcatc gaagatgcga ttaagtcgct gcgcatgcgt
     4501 ggtttcgata taggcctgga aatgtcgggt tctccgcagg ccttcgaaag catgatcgaa
     4561 aatatgtaca acggatcgaa gattgctctt ttgggtattt tgcccgattc tacccaagta
     4621 aactggaacc aaatcatatt caaggctttg accttaaagg gtatttatgg ccgcgaaatg
     4681 tgggaaacct ggtatcagat ggagcaaatg cttatcacag gcatcgattt gagcccggtt
     4741 attacacacc gttttgatgt agacgatttt caaaaggcct tcgatgtaat ggagtctgcc
     4801 gcctgcggca aagttatttt gaactgggcc tgaacacttt gaactgggcc tgaacactcg
     4861 tttaagacaa aggagagtcg ggctcggctt tcatcatttt gtattccatg cggctgacga
     4921 gcttcgggaa aaacttgttc accagcactg ccgctttacc caggggagtg agtatcatct
     4981 gatctttgcg tttgcgcagt ccccgtagca tatgacgggc cacggtttcg gccgacatca
     5041 ttttttcttc cttgcgggga gtctcgccct gagggctgcc atcggctgtc agggccgaaa
     5101 agcgcacatt ggaggctgta aaacccgggg caaaaatcat cacatgcaag tcgtcgtaca
     5161 gatgttcggt acgcagggtg tcgagaaagc cgttcatggc aaattttgag gccgaatagc
     5221 ctgtgcgggc gggcagtccc ttgaagccgg ctatggaagt aacgcccacc accgagccct
     5281 tgcgttcgag caggctgggc aaagcgtatt tggtgcaata gacacagccc cagaaattta
     5341 catccatcaa ttgatgcaag actttcaagt cgaggtcttt gaaaacggcc cgcatggaaa
     5401 taccggcatt gtttaccagg atatcgatgc ctccgtaggt tgctatggtt tgctctatga
     5461 gttttcggca atcttcttct ttgctgacgt cggttttaac gaccagcacg gttgtaggga
     5521 gttccttctg caagacttgc agtttgtcga gggatcttgc tgccaacgcg agttttgcac
     5581 cctgagcggc aaaaagccgg gcacaagcca ggcctatgcc cgacgacgct ccggtgatca
     5641 ctacaacttt gtctttaaaa tactgttgtt tcataacctc agtcttaaat gtttataaac
     5701 ccctgccgtg gcaatttttg tattttttgc ccgaaccgca gggacaggga tcgttgcgac
     5761 ccaccttttt ttctaccctg accggttcca gctggcgttg ctctctgtct gacccagctc
     5821 ggcgagccgg acttccgcca tccaaagagt ctctttgagc acggtattta ctgtagtcgt
     5881 ggcgtttttg cgtttcggct ttgcgcaccg attcgggttc gcgtatagga atttgtccgc
     5941 gcatcaaaat agagatactc ttcttattga tattgtcgat catggttttg aagaggttgt
     6001 acgactccag tttgtagata agaagagggt ctttttgttc gtagctggca ttttgtaccg
     6061 attgacgcaa ctcgtcgagc tcgcggagat tttctttcca ggcttcgtcg atggtatgca
     6121 acagaatggc tttttcgaac gtttttacca cttctttcga ctggctttct gcggcagcct
     6181 tcaggttaca ggggatgttg taagtgcgtt taccatcggt gatgggtatc aaaatgtttt
     6241 cgtattgggc agcctgcttc tcgtagacct gctgaatgac cggattgact atggaggcca
     6301 ttttttcggt tttacgcttg tagaggttca gggctgcttc gtatatttcg tctacgctgt
     6361 tttcggctct ctgatcgtaa aaatgctctt cgtccatggg gacttccata gccaggtatt
     6421 tgagtatgtc cattttgaga ccctcgaagt cgcgttggtc gtaagcttct tcgactaagc
     6481 ttttgatggt gtcgtaaatg gtgttggcca ggtgcatacc gatacgttct cccatcaggg
     6541 catggcggcg cctggtgtag atgacttcgc gctggttgtt cataacgtcg tcgtattcga
     6601 gtagacgttt acgtatgccg aagttgtttt cttccacttt tttctgtgcc ctttcgatgg
     6661 ctttcgaaat catggggttt tcgatgagct cgccttcttc caggcccatg cggtccatga
     6721 tgcccgaaat acgttccgat ccgaacaggc gcatcaggtc gtcttcgagc gatacgtaaa
     6781 acaccgaaga tccggggtcg ccctgacggc ctgaacgtcc gcgcagctga cggtcgacac
     6841 ggcgcgattc gtgacgttcc gtgcctatga tggccaaacc tccggccttt ctaacttctt
     6901 cggtgagctt gatgtcggta ccacgtcccg ccatgttggt ggcaatggtt accacgcctt
     6961 tttgtccggc gttggccacg atttcggctt cgcgctggtg tagcttggcg ttgagcacgt
     7021 catgcttgat tttacgtagt ttgagtgtac ggctcaacaa ttcggagatt tctaccgaag
     7081 tagtacctac cagcaccggt cgcccggctt cgaccaag
//
LOCUS       DAOSGL010000088         7035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_9314_length_7035_cov_4.197135, whole genome shotgun sequence.
ACCESSION   DAOSGL010000088 DAOSGL010000000
VERSION     DAOSGL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9314_length_7035_cov_4.197135"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1796
                     /locus_tag="PKY68_04065"
     CDS             108..1796
                     /locus_tag="PKY68_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24244.1"
                     /translation="MKISFLKGLFFTVMISVCFASCERMMETESSLLSYPEEHELNTS
                     ADTVYSVIGIMNKIQTLADRSVLLGELRADLVDVNENTISSLREIANFEISEDNPYNQ
                     PKDYYAVINNCNYFLQKADTALRKRNIPVFLREFAAVKAFRAWTYLQLVLNYGKVPFY
                     TQAILSEADAAQNFPEYGIAEICDYFIDDLLPYIDVPFPGYGSIADLDSRLFYIPIRL
                     LLGDLCLWSERYSEAAAYYHDYLNEEGLYTGTYRIEFTEDDLQFEGKILSNSYGAMFS
                     NLSAGADILAVIPMDSSVSSNAYSQLRNYFNSTIHNELEFQLLPSLRLQEISASQVFC
                     RAKQSGSPSYAPRANEENPLLAGDLRLYAYWSKTKVEGEDRQTIMKFTTDHVFTYRKG
                     LIYLRYAEALNRAGYPESAFAILKYGLNNINLVRFVSPAEYEVEGGIDPLLTFNPTLF
                     NNTNTLGIHSRGCGDSEYNEFYVLPTPPDSLESRQDTLDYRIERVEELILTEMALETA
                     LEGHRFYDLMRVALRRGQEAYLAEKVALRKGSDNRDDALYNSLLNKNNWYLKMK"
     gene            1805..4279
                     /locus_tag="PKY68_04070"
     CDS             1805..4279
                     /locus_tag="PKY68_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24245.1"
                     /translation="MNNILRVFRPSLPLIVLLAGMFFSCKDNNPLDESYPEWLGSSIY
                     GYLEKQGNYSVFLQLIDDLQYDQVLKLTGSKTLFVADDEAFDVFFNAENVWGVSSYSD
                     LSMAQKKLLLNSAMINNAYLIDKLSSVPGPEEGKCLRRTTAVSVYDSVPLVQAQELPD
                     TKYWQRFKDNDRSLLLMKDFTPVPMVHFLRRQLENNNITDEDFSIIFNGYNRQINDAH
                     INGIRVIDQNIVCQNGYVHKLEKVMLPFDNMAEVLRTHPATTKYSALMERFSAPYYSA
                     AATGNYQRIYGGTDSVFVKRFFSQRSAEGGNLTDPDGYATTGVLKFDPGWNTYYSESE
                     ELAMEQDMAAMLVPSNAALDFYWEHDGRALKDYYVVWDSVPDKVLVKLLNNNMLNSFN
                     ASVPSKFDKVTNDANDAMGLTIADVDCCLIANNGVIYITNKVFGPTSYISVSFPALIN
                     ESMSIINWAVEELEFNAYLNSQDSYYSFIIPVNDALKYYIDPVSYGKSKTKLFEFYYD
                     PTETNVVNRVKANKFTIDLDTWTKSDSSSATTAEIQNRLDDIVDNLIVIGDISDGRTY
                     YKTKAGGIIKVTDAHLGEGGMKIAGGYQVEQDTFVTVRTIYDQSVSGNGKSYIVEAPL
                     LSSKKSLYATLKEKPEFKVFYDLMEGSDFFVTQMNKHSCVDRNCNLFNAFNYTVYVPT
                     NESLQALLDDGTLPDWEKYELETDVEIKKAIKDSIESFLRYHIQDNSVYLDKAAINGN
                     FETSAINRETGRFYKLTVEGNGSQGLFITDLMGNRRRVLQSDPNLYNLMVREYLYDTA
                     DKEKATTIFASSYAAVHQIDAPLFFR"
     gene            complement(4357..5772)
                     /locus_tag="PKY68_04075"
     CDS             complement(4357..5772)
                     /locus_tag="PKY68_04075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOH24246.1"
                     /translation="MEKDKKYILRYALLDYIATLIAWQLFNIYRFYEVRSTVYFNTLG
                     DFLINTKAILMGIGMPLFWLIVYFYSGYYAKPRQKNHTSDLLSTLFSTLTGSLIIFFA
                     VIINDYPEQYTLYYRVFAGLFLIHFFCTWFVRFLQTSRLLNLHAEGKVGINVLIIGSG
                     KVAGKLSRQMQQTKNSSAYLLKGFIRVNSEEKAVAEQDILGDTEQLKEIIKEQRIEAL
                     ILATDRQDSDYTEGLLKQLYVYHLDILSVIHKQDLLFSNITLYSLPGVPLKYLSPTQL
                     KPWEQNLKRSTDILVSVLGLLVLSPLFVFLALRIRLESAGPILYRQERLGKEMKPFTI
                     LKFRTMFAHSEPEGPLLSSLNDERITPFGRFLRKYRLDELPQLWNVLKGEMSLVGPRP
                     ERRFYVERIVDQAPQYYLVYRVKPGITSLGMVKYGYADSVEKMIERLEYDLIYLKNQS
                     LLMDLKILIFTFKPIALGKGI"
     gene            complement(5795..6367)
                     /locus_tag="PKY68_04080"
     CDS             complement(5795..6367)
                     /locus_tag="PKY68_04080"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOH24247.1"
                     /translation="MSLQEEINKAGEVLKRGGIILYPTDTVWGLGCDAGNPQAIEKIH
                     AIKKRAENKAMIVLLDHPGKLDLYVEEVPELAWDLIELSDKPLTIIYQGAKNLAANLI
                     AEDGSVAIRITREQFSQSLCQSFRKAIVSTSANISGTPAPASFRDISAEIISAVDYVV
                     DYRRNEPAGKAKPSSIISLGNKGEIKIIRP"
     gene            6577..>7035
                     /locus_tag="PKY68_04085"
     CDS             6577..>7035
                     /locus_tag="PKY68_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH24248.1"
                     /translation="MSKWNLLTLTPEQETQKEQLAEELGISPILAKLLILRGISSFSE
                     AKKFFRPQLSDLHDPFLMKDMDKAIARLNLAIANKEKILVYGDYDVDGATAVALVYKF
                     LRQYSLNIEFYLPDRYVEGYGISYQGIDYAFEHQIKLIIVLDCGIKAVEKV"
BASE COUNT         1932 a         1558 c         1606 g         1939 t
ORIGIN      
        1 ttctcgagcg ggaatgaggt gctctgccag ggcattgacc gtggtatgct ggccttaggt
       61 cccagtgtgg ttttaggcat caaactcaac ctctaaatct cttaatcatg aagatatctt
      121 ttttaaaagg cctgtttttt acagtaatga tttcagtctg ttttgcctct tgtgaacgca
      181 tgatggagac agaatcttcg ttgctctctt atcccgaaga acacgagctt aatacttctg
      241 ccgacacggt ttattccgtt attggtatca tgaacaaaat tcagaccctg gcagatcgca
      301 gcgtgttact gggagagctc agagccgatt tagtggatgt aaacgaaaac accataagca
      361 gtctgcgtga aattgccaac tttgaaattt ccgaagacaa tccttataat caacctaaag
      421 actattacgc tgtaatcaac aactgcaatt attttcttca aaaagcagat acggcactgc
      481 ggaaacgcaa catacccgtt tttttgagag aatttgccgc agtcaaagcc tttcgtgcct
      541 ggacctatct tcaactggtg ctgaattacg gcaaagtgcc tttttatacc caagctatcc
      601 tgagcgaagc cgatgccgct caaaactttc ccgaatacgg catagcggag atctgcgatt
      661 attttatcga tgatctgctg ccttacattg atgtgccgtt tcccggctat ggcagtatag
      721 ccgatctgga ttccaggttg ttctatatac ccatacgact tttgttgggc gacctctgcc
      781 tctggtccga acgttacagc gaggctgcag cttactatca cgattacctg aacgaggaag
      841 ggctttatac cggaacatac cgtatagaat ttaccgaaga cgacttgcaa ttcgaaggaa
      901 agattctttc gaattcttat ggagctatgt tcagcaacct gtcggcgggc gcagatatct
      961 tagctgtcat ccccatggac tccagtgttt cctcgaatgc ttacagtcaa ttgagaaact
     1021 atttcaattc caccatccac aatgaattgg aattccagct tttgccttcg ttgcgtttac
     1081 aggaaatttc ggcttcgcag gtcttttgcc gggccaagca aagcggctct ccctcgtatg
     1141 ctccccgggc caacgaagaa aaccctctgc tggccggcga tttaaggtta tatgcgtatt
     1201 ggtctaaaac gaaggtcgaa ggcgaggaca gacagaccat tatgaaattt accaccgatc
     1261 acgtttttac ttatcgtaaa ggcttgatat atttgcgtta tgccgaagct ttaaaccggg
     1321 ccggctatcc cgaatcggct ttcgccattc tcaaatacgg tctgaacaac attaatctgg
     1381 ttcgttttgt ctctcctgca gaatacgagg tcgaaggcgg tatcgatccc ctgttgactt
     1441 tcaacccgac tttgttcaac aacaccaaca ccctgggcat tcattccaga ggctgcggcg
     1501 attcggaata caatgaattt tacgttttac ccactcctcc cgattccctc gagagtcggc
     1561 aggataccct cgattaccgg atcgaaaggg tagaagaact catattgacg gagatggcat
     1621 tggaaaccgc cttggaaggg caccgttttt atgatttgat gcgtgttgcc ctgcgccggg
     1681 ggcaggaagc ctacctggcc gaaaaagtgg ctctgagaaa aggcagcgac aatagagacg
     1741 atgctttata taacagcttg cttaataaaa acaactggta tctgaaaatg aaatgatcaa
     1801 cgctatgaat aatatactta gagtcttcag gccttctttg ccgcttatag ttttactggc
     1861 cggcatgttt ttttcctgta aagacaacaa tcccttggac gaaagctatc ccgaatggtt
     1921 ggggagcagc atctacggtt atctcgaaaa gcagggtaat tacagtgttt ttttgcaact
     1981 catcgacgac cttcaatacg atcaggtatt aaagctgacc ggcagcaaga ccttgtttgt
     2041 tgccgacgac gaggcttttg acgttttttt caatgccgaa aatgtatggg gtgtaagctc
     2101 atacagcgat ttaagtatgg cacagaaaaa gctcctgctg aattcggcca tgatcaacaa
     2161 cgcctatctg atcgacaaac tctccagcgt acccgggcct gaggagggta aatgcctgcg
     2221 ccgcactaca gccgtgtcgg tgtacgattc agtacccctg gtgcaagcac aggaattgcc
     2281 cgacaccaaa tactggcagc gtttcaaaga caacgatcgt tccctgttgc tgatgaaaga
     2341 ttttacgcct gtgcctatgg tgcattttct tcgtcgtcag ctcgaaaaca ataatattac
     2401 agacgaagat ttcagtatta tttttaatgg atacaaccgg caaatcaacg atgcacatat
     2461 caacggaatc agagtgattg atcagaacat tgtttgtcag aacggctatg tgcataaact
     2521 ggaaaaggtc atgttgcctt tcgacaacat ggccgaagtg ctcagaaccc acccggctac
     2581 gactaagtac agtgctctga tggagcgttt ttccgcccct tattacagcg ctgccgcaac
     2641 cggcaactat cagcgtatct atggcggcac cgattcggtt tttgtaaaac gttttttctc
     2701 ccaacgcagt gccgagggcg gtaaccttac cgatcccgac ggttatgcta ccacaggggt
     2761 gttgaaattc gatccgggtt ggaacaccta ttattcggag tcggaagaat tagctatgga
     2821 acaggatatg gccgccatgc tggtgccttc gaatgcagcc ctcgatttct actgggaaca
     2881 cgacggtcgt gcccttaagg attattacgt ggtttgggac agtgtgccgg acaaagtgct
     2941 ggtaaaactg ctcaacaata atatgctcaa ttctttcaac gcctctgttc ccagcaaatt
     3001 cgacaaagtc accaacgatg ccaacgatgc catgggactg accattgccg atgtggattg
     3061 ttgcctgata gccaacaatg gagtgattta catcaccaac aaagtctttg gccctaccag
     3121 ctatatttcg gtttcgtttc cggcattgat caacgaaagc atgagcatca tcaactgggc
     3181 ggtggaggaa ttggagttca acgcttacct aaattcccaa gacagctatt acagctttat
     3241 tataccggta aacgatgctt tgaaatatta catcgacccg gtttcctacg gtaaaagcaa
     3301 gaccaaattg tttgagtttt attacgatcc caccgaaaca aatgtcgtta acagagtaaa
     3361 agcaaataaa tttaccatag atctggatac ctggaccaaa tccgactcct cttcggccac
     3421 tactgccgaa attcaaaacc gtctcgacga tatagtcgac aatctcatcg tgatagggga
     3481 tatcagcgac ggacgcactt actacaaaac caaggcgggt ggaatcatca aagtgaccga
     3541 tgcccatctg ggcgaagggg gcatgaaaat tgccggaggc taccaggtgg aacaagatac
     3601 ctttgttacc gtccgaacca tttacgatca atcggtttcg ggtaacggga aatcttatat
     3661 tgtggaagct ccgttgctca gctccaaaaa gtctctttat gccactttga aggaaaaacc
     3721 cgaattcaaa gtgttttacg atctgatgga aggcagcgat ttctttgtaa ctcaaatgaa
     3781 caaacattct tgtgtcgaca ggaactgcaa tttgttcaac gcctttaact ataccgttta
     3841 cgtaccgacc aacgagtctt tgcaggcttt gctcgacgac ggcactttgc ccgattggga
     3901 aaaatatgaa ttggaaaccg acgtcgagat caaaaaagcc attaaggatt ctatcgaaag
     3961 tttccttagg tatcacattc aggataactc ggtttatttg gataaggccg ctatcaacgg
     4021 caactttgaa acttcggcca taaaccggga gacgggccgt ttctacaagc tgacggtcga
     4081 aggtaacgga tcgcaaggcc tgttcattac cgacctgatg ggcaatcgcc ggcgggtact
     4141 gcagagcgat cccaatcttt acaacctcat ggtaagagaa tacctgtacg acacggccga
     4201 caaagaaaaa gcaactacta tttttgcctc ctcctatgcg gctgtgcatc aaatagatgc
     4261 acctttattt tttagatagt tttttattgg agtgcttaga aaggcttgta agatcaggaa
     4321 ggaatcttac aagcctttgc tttatacctg catcgatcaa ataccttttc ccagcgctat
     4381 gggtttaaaa gtgaaaatca aaatctttag gtccattagc agggattgat tttttaggta
     4441 aatcagatcg tattccagcc gttcaatcat cttttctacc gaatcggcgt agccgtattt
     4501 taccatacct aaagaagtaa taccgggctt aactctgtaa accaggtagt attggggagc
     4561 ctgatccact atgcgttcca cgtaaaaacg cctttcgggt cgtggcccta ccagcgacat
     4621 ttctcctttg agtacgttcc aaagctgagg cagttcatcc aggcggtatt tgcgcaaaaa
     4681 acggccgaag ggagtaatac gctcgtcgtt gagcgaagag agtaagggcc cttcgggttc
     4741 tgaatgggca aacatggtcc tgaactttaa aatggtaaac ggcttcattt cttttcccaa
     4801 acgttcctgt cggtagagta tggggccagc ggattccaag cgtatgcgca aggccaggaa
     4861 gacaaagagg ggcgacagca ccagcaggcc caacacggaa accagtatgt cggtactgcg
     4921 tttaaggttt tgttcccaag gttttaactg tgtggggctc aaatatttca atggaactcc
     4981 cggcagagaa taaagagtaa tgttggaaaa aagcaagtct tgcttgtgga taacagacaa
     5041 aatatccaga tgatacacat aaagctgttt gagcaggcct tcggtataat cgctgtcttg
     5101 ccggtctgtg gccaggatta aagcctcgat acgttgttct ttgatgattt ctttgagttg
     5161 ctccgtatct cccaggatat cctgttccgc gacagccttt tcttcgctgt ttacgcgaat
     5221 gaaacctttc agaagatagg cagaactgtt tttggtttgc tgcatctggc ggctcagttt
     5281 gccggccact tttccgctgc ctataatcag gacattaata cccactttac cctcggcgtg
     5341 taaattaagc agcctggacg tttgtaaaaa acggacaaac caggtgcaaa aaaaatgtat
     5401 gagaaacaag ccggcaaaca ctctgtaata gagtgtgtat tgttcggggt agtcgttgat
     5461 gatgacggca aaaaagataa tcagcgatcc ggtcagggtg ctgaacaggg tgctcagtaa
     5521 atcggaggtg tggtttttct gtcgcggctt ggcgtaataa ccggaataaa agtagacaat
     5581 cagccagaac aaaggcattc ctatacccat cagaatagcc ttggtgttta ttaaaaaatc
     5641 ccccagggtg ttgaagtaaa cagtggaacg aacttcgtaa aaacggtaaa tattaaatag
     5701 ttgccaggct atcagagtgg ctatatagtc cagcaaagca tagcgcaata tgtatttttt
     5761 atctttttcc attgtggtgt tttatgccct gtttttaggg tcttattatt tttatttctc
     5821 ctttgttgcc caaacttatg atagaggaag gcttggcttt gcctgcgggc tcatttcgtc
     5881 tgtaatcgac cacataatcg acggccgaga tgatttctgc ggagatatcc ctgaaagaag
     5941 cgggagcagg cgttcccgag atattggccg aagtagatac gatggctttg cgaaaagact
     6001 ggcataaact ttgtgaaaat tgttcccggg tgattctgat ggccacgctg ccgtcttcgg
     6061 cgatcaggtt tgcagccagg tttttggctc cttggtaaat aatggtgagc ggtttgtcgg
     6121 acaattctat taagtcccat gccagctcgg gaacttcttc aacgtataaa tcgagtttac
     6181 cgggatggtc cagaagcaca atcatagctt tattttcggc tctttttttg atggcgtgga
     6241 ttttttcgat tgcctgcgga ttgccggcat cgcaacccag gccccaaacc gtatcggtgg
     6301 gatagagtat aatacctccc cgtttcagca cttctccggc tttgttaatt tcctcctgca
     6361 aactcatggt ttttttgcgt tgttttagtg tataacgtta tgcaccacaa agatagtatc
     6421 taattcttgt ttattgaata aaattcacat ggaatacaat ctgaatgacg gaataaaaac
     6481 gtatttttgt aagataattt tttgaaactg cggggagtgt tctctgttca gtttaaaaaa
     6541 ttctgcgaga acaaaagaaa aataatatat aatctaatga gcaaatggaa tttattaacc
     6601 ttaacccccg aacaagagac acagaaagaa caattagctg aagaactcgg tattagcccg
     6661 atattagcaa agttgctaat actgagagga ataagcagtt ttagtgaagc aaaaaaattt
     6721 tttagacctc aactgagcga tttgcacgac cccttcctga tgaaggatat ggacaaagct
     6781 attgccaggt tgaatttggc tatcgctaac aaagaaaaaa ttcttgtcta tggggattat
     6841 gatgtagacg gggctaccgc ggtagccttg gtttataagt ttttacgcca atacagtttg
     6901 aatatcgaat tttacctgcc cgatcgttat gtggagggtt acggtatttc ctatcaggga
     6961 atcgattatg cctttgagca ccaaataaaa ctgattattg tgttggattg cggcattaaa
     7021 gccgtggaaa aagtg
//
LOCUS       DAOSGL010000089         7010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_9358_length_7010_cov_4.311287, whole genome shotgun sequence.
ACCESSION   DAOSGL010000089 DAOSGL010000000
VERSION     DAOSGL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9358_length_7010_cov_4.311287"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3644
                     /gene="rpoB"
                     /locus_tag="PKY68_04090"
     CDS             <1..3644
                     /gene="rpoB"
                     /locus_tag="PKY68_04090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOH24249.1"
                     /translation="SESFPITDTRNNFVLEFIDYYVDPPRYTIEECIERGLTYSVPLK
                     AKLKLYCTDPDHEDFETKFQDVYLGAFPYMTPKGTFVVNGAERVIVSQLHRSPGVFFG
                     QSVHANGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFETDK
                     DILEIFNLAEEIKVTKTGLKKHVGRKLAARVLKSWVEDFVDEDTGEVVSIERNEIIID
                     RETVLESSHIDDILESGTPTVLLHKEDPDMSDFSIIYNTLQKDPSNSEKEAVLYIYRQ
                     LRNAEPADDSSAREVIQNLFFSEKRYDLGEVGRYRINKKLQLNTDMDTRVLTKDDIIH
                     IIRYLIELVNSNAIVDDIDHLSNRRVRTVGEQLNNQFGVGLARMARTIRDRMNVRDNE
                     VFAPVDLINAKTVSSVINSFFGTNALSQFMDQTNPLAEMTHKRRLSALGPGGLSRERA
                     GFEVRDVHYTHYGRLCPIETPEGPNIGLISSLCVFAKINKLGFIETPYRKVSNGIVEK
                     EVVFLTAEEEENLVVAQGNALLNEDGSFVRNRIKARLEADFPVVEPNKVDLMDVSPSQ
                     IASIAASLIPFLEHDDANRALMGSNMMRQAVPLIRCEAPIVGTGLEKTLIKDSRTQLM
                     AEGDGVVEYVDATSIHIRYDRTEDEEFVSFDDAVKIYNVPKFQKTNQSTTIDLKPIVR
                     KGQRVTKDQLLTEGFATEKGELALGRNLKVAFMPWKGYNFEDAIVLNERLVREDIFTS
                     VHVDEYTLEVRETKRGMEELTADIPNVSEDATKDLDKNGIIRIGAHVKPGDILIGKIT
                     PKGESDPSPEEKLLRAIFGDKAGDVKDASLKATPSLSGVVIDKRLFSKAIKNRKTRLT
                     DKAILPKLDEEFEEEVAAIRAVLLEKLWVLVSGKTSQGIKDNLDTEVIPKGVKFTQKI
                     LNEINYSSIQLSKWTSDNHKNDMIRDLIINYLKKEKELDAKLKRKKFNVTIGDELPAG
                     IIQMAKVYVAKKRKIKVGDKMAGRHGNKGIVSRIVRQEDMPFMEDGTPVDIVLNPLGV
                     PSRMNLGQIFETVLGWAGQKLGVKFATPIFDGAELEDLEQWSDKAGLPRYGKCYLYDG
                     GTGDRFDQPATVGVIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEM
                     EVWALEAFGASHILQEILTIKSDDVAGRSKAYEAIVKGEPMPQPGIPESLNVLLHELR
                     GLGLSIELD"
     gene            3667..>7010
                     /gene="rpoC"
                     /locus_tag="PKY68_04095"
     CDS             3667..>7010
                     /gene="rpoC"
                     /locus_tag="PKY68_04095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOH24250.1"
                     /translation="MALKKDIKTKTNFSKISIGLASPEEILELSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVVHIWYFRSLPNKIGYLLGLPTKKLDSVIYHERYIVIQPGILEEEEGVKVNDLL
                     SEEEYLALMDKLPRENQMLEDSDPQKFIAKMGADAVHDLLSRLDLDALSFDLRHRADT
                     DTSMQRKNDALKRLQVVEAFRAARHRNKPEWMVIKIVPVIPPELRPLVPLDGGRFATS
                     DLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSSAIKTDSN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKP
                     FIIRKLIERGIVKTVKAAKKFVDRKDPVVWDILEHVMKGHPVLLNRAPTLHRLGIQAF
                     QPKMIEGKAIQLHPLACTAFNADFDGDQMAVHLPLGNEAIAEAQLLMLSSHNILNPAN
                     GAPITVPSQDMVLGLYYMTKERIGAQGEGLTFYSPEEAKIAYNEKKVSLHAVVKVRIE
                     GELIQTTIGRILVNEYVPEEVGFVNTVLSKKELRNVIGKVIASCGVARTAQFLDDIKD
                     LGYSMAFQGGLSFNLDDVIVPDEKTELIQGGYEEVEAIMEEWRQGFITPNERYNKIID
                     VWTIVNNKLSNILIKQLAEDDQGFNSVHMMMDSGARGSKEQIRQLSGMRGLMNKPQKF
                     GAEGGQIIENPILANFKEGLSVLEYFISTHGARKSLSDTALKTADAGYLTRRLVDVSH
                     DVIVNEEDCGTLRGLTALEIKGVASLYERILGRVSVHDVFNPDTNELLVSAGQEITED
                     IAQKIQDSLVDRVEIRSVLTCESKKGVCAKCYGRNLATGRMVEKGEAVGVIAAQSIGE
                     PGTQLTLRTFHAGGVAKSENVENKLVAPYDALVEIDELRVVPSFEDKNVRIVVGRYAE
                     IHLIDPVTNMVTTHNLSYGSRLYVDPGDMVKQGDLICEWDVYNAVIICEQSGKIEFES
                     IEEGVTYQTYMDETTKIKDKIIIESKDRSHVPTIHIMDDKGQRLYSYNLPVGAHLLKE
                     SGDRIEAGQVLVKIPRAIPKSGDITGG"
BASE COUNT         1993 a         1588 c         1658 g         1771 t
ORIGIN      
        1 tttccgaaag ttttcctatc acggatacac ggaataattt tgtccttgaa tttatcgatt
       61 actacgttga tcctcctcgt tataccatcg aagaatgtat tgaacgggga ttgacttaca
      121 gcgttccact gaaggcgaag ctcaaacttt attgtaccga tccggatcac gaagattttg
      181 aaaccaaatt tcaggatgta tacctgggtg cgtttcccta catgaccccc aaaggcactt
      241 ttgttgttaa tggtgccgaa cgtgtaattg tatcgcagtt acatcgttca cccggggtat
      301 tttttggtca aagcgtgcat gccaacggga cgaaacttta ttccgcccgc attattccct
      361 tcaaaggctc gtggatagaa ttcgccacag acatcaacaa cgtgatgtat gcctacatcg
      421 accgtaagaa gaaattaccc gtaactactc tattgcgtgc catcggtttt gaaacagaca
      481 aagatattct ggaaattttc aacctggccg aagaaatcaa agtaaccaag accggtttaa
      541 aaaaacacgt cggccgtaaa ctggctgccc gtgttctgaa aagctgggtg gaagactttg
      601 tggatgaaga caccggcgaa gtggtttcca tagaaaggaa cgaaataatc attgacaggg
      661 aaaccgttct tgaaagcagt catattgacg acattcttga atctggaaca cctactgtcc
      721 ttctgcacaa agaggatccc gatatgtccg atttcagcat catatataat acgctgcaaa
      781 aggatccgag taattcagag aaggaagccg tgctttatat ttaccgtcaa ttgcgtaatg
      841 cagaaccggc cgacgattcc agtgcccgcg aagtaatcca gaatttgttc ttctccgaaa
      901 aacgttacga cctcggagaa gtgggacgtt accggattaa taagaaactc cagttgaata
      961 cggatatgga taccagggtc ctgaccaaag acgatattat tcatatcatc cgttatttaa
     1021 tcgaactggt caattccaac gccattgtgg acgatataga ccacttgagc aacagacgtg
     1081 tgcgcaccgt cggcgagcaa ctcaacaatc agtttggcgt gggccttgcc cgcatggcaa
     1141 gaaccattcg cgacagaatg aatgtacgcg acaacgaagt gtttgctccg gttgatctaa
     1201 tcaacgccaa aacggtatca tcggttatta attccttctt tgggaccaat gccttgtcgc
     1261 agtttatgga tcaaaccaac cccctggccg aaatgaccca taaacgccgc ttgtcggcct
     1321 tgggtccggg cggtttatcg cgtgaacgtg ccggttttga agtccgcgac gtgcattaca
     1381 cccactacgg gcgtctttgc cccatcgaaa cacccgaagg tcccaacatc ggtttgattt
     1441 cgtctttgtg tgtgtttgca aaaatcaata agcttggttt tatcgaaact ccctaccgca
     1501 aagttagcaa cggtatagtc gaaaaagagg tggttttctt aaccgcagaa gaggaagaaa
     1561 atctggtagt ggcacaaggt aatgctctct tgaatgaaga cggttctttc gttcgcaaca
     1621 gaattaaagc tcgtctcgaa gccgacttcc cggtagttga acccaataag gttgacctga
     1681 tggacgtttc tccttctcaa atagcctcta ttgcggcctc tttgattcct ttcctggaac
     1741 acgacgatgc caaccgcgct ctgatgggtt cgaacatgat gcgtcaggcc gtgcccttga
     1801 tacgttgcga ggcaccgata gtaggtaccg gtctggaaaa aactctgata aaggattccc
     1861 gtactcaatt gatggccgaa ggcgatggag tggtcgaata tgtggatgct acaagcattc
     1921 atattcgtta cgaccgtacc gaagacgaag aatttgtgag tttcgacgac gctgtcaaaa
     1981 tctacaacgt tcctaaattc caaaaaacga accaaagcac caccatcgac ctgaaaccca
     2041 ttgttcgtaa gggacaaaga gtgactaaag atcagctttt gaccgaaggc ttcgctaccg
     2101 aaaagggcga attggctctt ggccgcaacc ttaaagtggc ttttatgccc tggaaaggct
     2161 ataacttcga agacgccatc gtgttgaacg aacgcttggt acgcgaagat atttttactt
     2221 ctgtgcatgt cgatgagtac actctcgaag ttcgcgaaac caagcgcggc atggaagaat
     2281 tgactgcaga tattcccaac gtcagcgaag acgccaccaa agacttggac aaaaacggta
     2341 tcattcgtat cggtgctcat gttaagcccg gtgacattct tatcggaaaa attactccca
     2401 agggagaatc cgatccttca ccggaagaaa aactcttaag agctattttt ggagataaag
     2461 ccggtgatgt taaggatgcc tctctgaagg caactccttc tctcagtggt gtagttatcg
     2521 acaaacgtct cttctccaaa gccattaaga accgcaagac ccgcttgacc gacaaagcca
     2581 ttctgcctaa acttgatgaa gaattcgaag aagaagtagc cgccatacgt gccgtattgc
     2641 tcgaaaaact ctgggttttg gtaagcggta aaacttctca gggaatcaaa gataatctgg
     2701 ataccgaagt catacctaaa ggcgttaagt tcactcaaaa gatcctgaat gaaatcaact
     2761 attcttccat tcagttgagt aaatggacct ccgacaatca caagaacgat atgattcgcg
     2821 atctgattat caattacctc aagaaggaaa aagaattgga tgcgaaactc aaacgtaaaa
     2881 agttcaatgt gaccataggc gacgagttac ccgccggcat cattcagatg gccaaggttt
     2941 acgttgccaa aaaacgcaaa atcaaggttg gtgacaagat ggccggacgt cacggaaaca
     3001 aaggtatcgt ttcgcgcatt gttcgccagg aagatatgcc ttttatggaa gacggaactc
     3061 cggttgatat agtcttgaac cctcttggcg taccttcgcg tatgaacctg gggcaaattt
     3121 ttgagacggt attgggatgg gccggacaaa agttaggcgt taaatttgcc acgcctattt
     3181 ttgacggtgc cgagttggaa gatctggaac aatggtccga caaagccgga ttaccgcgtt
     3241 acggtaaatg ctatctctac gatggcggca ccggcgatcg ttttgaccaa ccggccacgg
     3301 tgggtgtaat atatatgctt aaactcggcc acatggttga tgataagatg catgcccgtt
     3361 ccattggacc atactccctg attacccagc aacctctggg tggtaaggct cagtttggcg
     3421 gccagcgttt cggagaaatg gaagtttggg cgctcgaagc ctttggagct tcgcacatac
     3481 tgcaggaaat cctgaccatt aagtcggacg atgtagccgg ccgcagcaaa gcttatgaag
     3541 caatagtcaa gggagaacct atgccgcagc ccggtattcc cgaatccctg aatgtattgc
     3601 tgcacgaact cagaggcctg ggtctaagca tcgaattaga ttaacaactt caattacaca
     3661 cagaatatgg ctctcaaaaa agatataaag accaaaacaa acttctcgaa aatctctata
     3721 ggattggctt cgcccgaaga aattcttgaa ttgtcgagtg gtgaagtatt gaagccggaa
     3781 accattaatt atcgtaccta taaacccgaa cgcgacggtt tgttttgcga aaggattttt
     3841 ggtcccgtaa aagattatga atgtcattgc ggtaagtaca agcgtatccg ttacaagggc
     3901 atagtttgcg accgttgcgg ggtagaagtt acagaaaaga aagtacgtcg tgaacgtatg
     3961 gggcacatac agctggtagt cccggttgtt cacatttggt atttccgttc actacccaac
     4021 aaaattggct atttgctggg tttacccacc aaaaagctgg attcggttat ctaccacgaa
     4081 cgttatatag tcatccagcc cgggatactc gaagaggaag aaggtgtaaa ggttaacgac
     4141 ttattatcgg aagaggaata tctggctctc atggataaac tgcccagaga aaatcaaatg
     4201 ctcgaggaca gcgatccgca gaaatttatt gccaaaatgg gtgccgatgc agttcacgac
     4261 cttttatcca gactcgatct ggatgcactg tcgtttgatt tgcgtcaccg tgccgacaca
     4321 gacacctcca tgcagcgtaa gaacgatgcg ctcaaacgtc tgcaggtggt agaagctttc
     4381 cgtgcagcca gacacagaaa caaacccgag tggatggtca ttaagattgt cccggtcatc
     4441 cccccagagt tgcgtcccct ggttcctttg gacggtggtc gttttgcgac ttccgacctg
     4501 aacgatctct accgtcgtgt tatcatacgc aacaatcgtc tgaaacggct tatagaaatt
     4561 aaagctccgg aggtgatatt aaggaatgaa aagcgcatgc tgcaggaagc tgtcgattct
     4621 ttgtttgaca actcccgcaa atcaagcgcc ataaaaaccg attccaaccg tccgcttaag
     4681 tctttgtccg attcgctcaa aggtaaacaa ggccgtttcc gtcagaatct gttgggaaaa
     4741 cgtgtcgatt actcagcccg ttccgttatt gtagtcggtc ctgaattgaa aatgcacgaa
     4801 tgcggattgc ccaaggatat ggctgcagaa ttatacaaac cgtttatcat acgtaaactg
     4861 attgaacgtg gaatagtaaa aaccgtaaaa gccgccaaaa aatttgtaga ccgcaaggat
     4921 ccggtggttt gggacatcct ggaacacgtc atgaaaggcc atcccgtact gcttaaccgt
     4981 gcccctactt tgcatcgtct cggtattcag gcattccagc cgaaaatgat agaaggcaaa
     5041 gcaattcagt tacatcctct tgcttgtacg gcttttaatg ccgacttcga cggagaccag
     5101 atggctgtgc accttccctt gggtaacgaa gctattgccg aagctcaatt gttgatgttg
     5161 tcttcgcaca acattctgaa tccggccaat ggagctccca ttaccgttcc gtcgcaggat
     5221 atggtgttgg gtttgtatta tatgaccaaa gagcgaatag gggctcaggg agaaggattg
     5281 actttctatt ctcccgaaga agccaaaata gcctacaacg agaaaaaggt ttctttgcat
     5341 gccgttgtaa aagtgcgtat tgaaggtgaa ctgatacaga ctaccatagg acgtattctc
     5401 gtaaacgaat atgtgcccga agaagttggt tttgtaaata ccgtcctttc caaaaaagaa
     5461 ctgcggaacg taattggtaa agtcattgct tcctgcggag tggcacgtac tgcccagttc
     5521 ctcgatgata tcaaggattt gggttattct atggctttcc agggaggttt gtccttcaac
     5581 ctggacgatg tgatcgttcc ggacgaaaaa accgagttaa ttcagggagg ttatgaagaa
     5641 gtcgaagcca ttatggaaga atggagacag ggttttatca ctcccaatga acgttacaat
     5701 aagattatcg acgtttggac cattgtaaac aacaaacttt ctaatattct cattaagcag
     5761 ttggccgaag acgatcaggg tttcaattcc gtacacatga tgatggattc cggagctcgc
     5821 ggatcgaaag aacagattcg ccagttgtcc ggtatgcgcg gcttaatgaa taaacctcaa
     5881 aaatttggcg ctgagggtgg acaaatcatc gaaaacccca ttttggccaa ctttaaagaa
     5941 ggtttgtcgg tgctggagta ttttatttct actcacgggg ctcgcaagtc tttgtctgat
     6001 actgcactaa aaacagctga tgccggatat cttacacgtc gtttggttga tgtatctcac
     6061 gatgtcattg tcaacgaaga agattgtgga accctgagag ggcttacagc cttggaaatc
     6121 aaaggggtag catcacttta tgaacgcatt ctcggacgcg tttccgtaca cgatgttttc
     6181 aacccggata ccaacgagct tttggtgtca gccggccagg aaatcacgga agacattgca
     6241 cagaaaattc aggactctct cgtcgaccgg gtggaaatcc gttcggtatt gacctgcgaa
     6301 tccaaaaaag gagtttgcgc caaatgttat ggccgtaatt tagccaccgg acgtatggta
     6361 gaaaaagggg aggctgtggg cgttattgct gcacaatcca ttggtgaacc gggaactcag
     6421 ttgacactgc gtactttcca tgccggtggt gttgccaaga gcgaaaatgt tgaaaacaaa
     6481 cttgtagcac cttacgacgc cctggttgaa attgatgaat tacgggtagt accctctttc
     6541 gaagacaaaa acgtcaggat cgtagtaggc cgttatgctg aaatccacct gatcgaccct
     6601 gtcaccaata tggttaccac acataacctt tcttacggat cacgtttata cgttgatccc
     6661 ggggatatgg ttaaacaagg cgatctgata tgtgaatggg atgtctataa tgcggtaatc
     6721 atctgcgaac aatcgggtaa aatcgaattc gaaagcattg aagaaggtgt cacttatcag
     6781 acttatatgg atgagaccac caagattaag gataagatta tcatcgaatc caaagatcgt
     6841 tcccatgtgc ctaccatcca tattatggac gataagggtc aacgcctgta ctcctataac
     6901 ctgcccgtag gcgctcacct gctcaaagaa tcgggcgacc gtatcgaagc aggccaggtg
     6961 ttggtcaaga tcccgcgtgc cattcccaaa tcgggcgaca tcaccggagg
//
LOCUS       DAOSGL010000090         7005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_9368_length_7005_cov_3.766906, whole genome shotgun sequence.
ACCESSION   DAOSGL010000090 DAOSGL010000000
VERSION     DAOSGL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9368_length_7005_cov_3.766906"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3574
                     /locus_tag="PKY68_04100"
     CDS             <1..3574
                     /locus_tag="PKY68_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOH24251.1"
                     /translation="VYFNGNVNLNEIGDEKQFKLLSAVDFLHLQPEALHLIQAVFSIQ
                     DSVIPPVVYKLGEMDFCGVIALEPENWNITGEISANPGKLSILTQMEYSSSDAVYDFN
                     LHLNSDSYALNKLFDSRITLGKTAFEIDLNGKKAGIDPPIADLNAIFKTLQINNYTYH
                     TILLDAYYNGHGFYNANLNSKDKNCLLDLAISLNDLGDEAAISCNLNAHRIDLQSLNW
                     MPAAEEDKGSELSFNMQADLKGNTLQQLRGRLNIDSLRFENNGELFELDNFYALSGEN
                     GGERYVLLKSDLLEGWMYGYIDMTNLLEDARNKALLQYFPTLFPDIKGDTRSMNNYDF
                     RFKLDDTRSLSRALNLPLSLPSGMVFDGYFRDESALFALNLNSEAIELDGWRIRSLKT
                     TLDNPSDSLQWHFSGILEREDTSRINVDFAARALNNELYWETHWHNDTGSVYKIYLSN
                     ILRFERKNPKSPLSIFSALNPSEFVVRDSLWKMESSDLQWDGKKLIVNNFLVHHNNQH
                     IKLDGTLAAGNEEEFLVDLSEVNLDYIFELLPRRREKPLLLGGSISGAAQIINLFEEP
                     KLNASLSVENFSLNKAVLGDLNVNSSWNEEISGIEMNGILLDGGLQVAEAKGAIFPAA
                     DSIYLWIDAEKARLSFIGSFIEDVIENLDGYGSGYVEIAGTFSNEKVGVTSDCYVEEG
                     VIGVDLLHCNYYFNDSLHLTPQGIFFKNINARDNEGNPAVINGTIRHNYFKDFALDLN
                     IQTEKLKVFDIEPSYSEPFYGRVYATGTAMLNGPLEDIQMDINMRTDERSSFAITLSE
                     ESEVSDYSFIEFVDRDKEAPKDSINEEQRLLIPLTRKTAETPEGRLNLNLQIEATPSA
                     EIILITNPTTGDEIRGRGNGSIRLVYDNIGDIELYGRYTLESGSYQFIIQDLLRRDFN
                     ILQGSTINFSGDPLEAEMNINAIYSVVNVSLSDLLDEAEIAALNLNRSSIPVNCSLLL
                     TGELQHPNIELGLDFPSADEELKRRVMNVINTDEMLNRQIVYLMLLNRFAAAENTQAQ
                     AGNNVNAVVGATLSSLSNQLNNMIYQALGSSFLTFDFNYRYDDLVSQGLGEWQLAMSS
                     QLLDNRLIINGNIGSREDLVSNNTQFIGDFDLEYKFSQSGRWRLKMFNRSNDNRYFKS
                     AMTTQGVGLGYRESFNNLLELRQLFAERIADQIIKSLKESDLKK"
     gene            complement(3578..5059)
                     /gene="proS"
                     /locus_tag="PKY68_04105"
     CDS             complement(3578..5059)
                     /gene="proS"
                     /locus_tag="PKY68_04105"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOH24252.1"
                     /translation="MAKELKELCSRSENYSQWYTDLVIKADLAENSAVRGCMVIKPYG
                     YSIWEKMQAQLDKMFKETGHQNAYFPLFIPKSFLSREAEHVEGFAKECAVVTHYRLKK
                     SPDGNQLIVDPEAKLEEELVVRPTSETIIWSTYKDWIQSYRDLPILCNQWANVVRWEM
                     RTRLFLRTAEFLWQEGHTAHATKQEAEEEAIKMLNLYADFAEKYMAMPVLKGVKTANE
                     RFAGALETYTIEALMQDGKALQSGTSHFLGQNFAKAFDVTFLNKENKLEYVWATSWGV
                     STRLMGALIMSHSDDNGLVLPPALAPFQVVIVPIYKNAEQLKEIDEKVSNIMLILKDL
                     GISVRYDNNDNKKPGWKFSEYELKGVPVRLALGARDLQNNTIEVARRDTLSKETRPME
                     GIGLYVKELLEDIQQNIYRKALDYRSKNTFEVNSYDEFKEKIEEGGFLSCHWDGSPET
                     EERVKNETKATIRCIPLDGDQTPGTCIFSGKPSARRVIFARAY"
     gene            5180..6127
                     /locus_tag="PKY68_04110"
     CDS             5180..6127
                     /locus_tag="PKY68_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOH24253.1"
                     /translation="MLESLSALDQVTINFSSAGTHLVNSILALIMFGVALDIRLGVFK
                     EVLVKPKALITGLLSQLIMLPAVTFLLIIVFHKWLTPTVCMGMILVSACPGGNISNFM
                     TNLARGKTELSVTMTAINTALAIFTTPLIYKIYGGLYVDYMSRASGNLLQPIVIPLGQ
                     MFYTIFILLGIPLTLGLLCTHFFPKFSQKISPGFRRVSMALFLLIVVMAFSNNFSLFL
                     KHIQWIFIIVLVHNGLALLTGFSFSSLLGVNRGERRAITIETGIQNSGLGLALLFNPK
                     IFPPEMAIGGMLFITAWWGIWHIVSGLSLSYLWSKKPLD"
     gene            6197..>7005
                     /locus_tag="PKY68_04115"
     CDS             6197..>7005
                     /locus_tag="PKY68_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOH24254.1"
                     /translation="MFYILLRFYTRICFRCFYRNIYYTGWENIPKNGPVIFAPNHQNA
                     LMDALAVLLWQKKPVVFLARADMFKKKFIGHLLRLVKIMPVYRMKDGYSKLTKNEQQF
                     KEASCVLINNGYLCLMPEGGQDGHRRLRPLVKGIFRIAFEAQLAVGKSRDVQIVPVGI
                     DYSEYEHAGSDLVVHFGKTILVSDYIGFYEQNPVLGMNTLRDHLAGKMKSLIIDIRNT
                     EYYEAIYGLSRLLSPAYIRAGVLKDSAINRFKARRRLSGRLEKFFENPEKQ"
BASE COUNT         1959 a         1449 c         1520 g         2077 t
ORIGIN      
        1 ggtgtatttc aatggcaacg taaacctgaa cgaaatcgga gatgaaaaac aatttaagtt
       61 gctgtccgct gtggactttt tacatctgca accggaagcc cttcacctga tacaagctgt
      121 tttttcgata caagacagcg tcataccccc tgtggtttac aagttgggtg aaatggattt
      181 ctgcggagtg attgctctgg agcctgaaaa ttggaatata accggtgaaa tatcggccaa
      241 ccccggaaag ttgtcgatcc tgactcagat ggaatattct tcttcggacg ctgtttatga
      301 tttcaatctg cacttgaatt ctgattccta cgctctgaac aagttatttg atagtcgtat
      361 taccttaggg aaaacagctt tcgaaataga cctaaatgga aaaaaagccg gaatagaccc
      421 tcctattgcc gacttaaacg caatattcaa aaccctgcaa ataaacaatt acacttatca
      481 taccattttg ctggatgctt actacaacgg tcacggattt tacaatgcca acctgaactc
      541 caaggataaa aattgtttat tggatttagc catatcactg aatgatttgg gcgacgaagc
      601 tgccatcagt tgcaatctga atgcgcatcg tattgattta caaagcttaa attggatgcc
      661 ggccgcagaa gaagataaag gttcagaact gtcttttaat atgcaagccg atttaaaagg
      721 taatacattg caacaactga gaggacgttt aaatatagat tctctgcggt ttgaaaacaa
      781 tggtgaattg tttgagctgg ataattttta cgcattgagc ggtgaaaatg gaggagaacg
      841 ctatgtatta ctgaaatccg atctgctcga aggttggatg tatggataca ttgatatgac
      901 aaacttgctg gaagatgctc gaaacaaggc attgttgcag tactttccca ccctttttcc
      961 ggacataaag ggggatacgc gcagcatgaa caactatgat ttccgtttta agcttgacga
     1021 taccaggagc ttgtcacgag cattgaattt acctttgagt ctgccctccg gcatggtttt
     1081 cgatggttat ttcagagatg agtcggcatt atttgcactc aatctgaatt ctgaagctat
     1141 tgaactggac ggatggcgca tccgttctct taaaactacg cttgataatc cttccgattc
     1201 cctgcaatgg catttttcgg gaatacttga acgcgaggat accagcagga taaatgtgga
     1261 ttttgcagcc agggccctga ataacgaatt gtattgggaa acccattggc acaacgatac
     1321 cggatcagta tataaaattt atttgagcaa tattttgcga tttgaacgga agaatcctaa
     1381 atcgcctttg agcatatttt ccgctttaaa tccttcggaa tttgtagtga gagactcctt
     1441 atggaaaatg gaatcatcag atttacaatg ggatggaaaa aaattgatag taaataattt
     1501 tctggtacat cacaacaacc aacacataaa actcgatgga actttagctg caggcaatga
     1561 ggaagagttt ttggttgatt taagtgaggt caatctggat tacatttttg aattattgcc
     1621 tcgccggaga gaaaaacccc tgcttttagg aggaagtatc agcggcgctg cacagataat
     1681 caatttattt gaagaaccta aattaaatgc ttcattgagt gttgaaaatt tttctctcaa
     1741 caaagcagtt ctgggagatt taaacgttaa ttcaagctgg aatgaagaga tttccggcat
     1801 agaaatgaac ggcattttgt tagacggagg attacaggta gccgaagcaa aaggtgctat
     1861 attccctgcc gccgattcta tttacttgtg gatagatgcg gaaaaagcga gactctcttt
     1921 tataggaagc tttattgaag atgttattga aaatttggat ggttacggca gcggatacgt
     1981 cgaaatagcc ggaacgttta gtaacgaaaa ggttggtgta accagcgact gctatgtgga
     2041 agagggtgta atcggggtgg atttactcca ctgtaactac tattttaacg actccctaca
     2101 cctgacgcct cagggtatct ttttcaaaaa tatcaatgcc agagataacg agggaaatcc
     2161 ggctgtaata aacggcacca tcagacacaa ctattttaaa gactttgctc tggatctgaa
     2221 tatacaaacc gagaaactaa aagtttttga catcgaacct tcctattcgg aaccattcta
     2281 cggccgggtg tatgccaccg ggacagccat gttgaatggc cctttagaag acatccagat
     2341 ggacatcaat atgcgcaccg acgaacgcag tagttttgcc attacactgt ctgaggaatc
     2401 ggaggtcagc gactacagtt tcatagaatt tgtcgaccgc gacaaggagg ccccaaaaga
     2461 cagcattaat gaagagcaaa gactattgat acctttaacc aggaagactg ccgaaactcc
     2521 agaaggcaga cttaatctaa atctacagat tgaggctaca ccttctgccg aaattatttt
     2581 gataaccaat cctactaccg gtgacgagat cagggggcgt ggaaacggaa gcatccgcct
     2641 ggtgtacgat aatatcggcg atattgagct ctacgggcgt tacaccctcg aatcgggcag
     2701 ttatcagttt attatccaag accttttgcg gagagatttc aacattttgc agggaagcac
     2761 cattaatttt tccggcgatc ctctggaagc ggaaatgaat attaacgcga tttattcggt
     2821 ggtaaatgta tcacttagcg atttgttgga cgaagcagaa attgctgcct taaatttgaa
     2881 tcgcagcagt attccggtca attgcagttt gttgttaacg ggagaactac aacatcccaa
     2941 catcgaattg gggctggatt ttccttcggc tgacgaggaa ttgaaacgcc gggtaatgaa
     3001 tgtaatcaac accgatgaaa tgctcaaccg gcaaattgta tatttgatgc tattgaaccg
     3061 ttttgcagcc gccgaaaaca cccaggccca agccggcaat aacgtaaatg cggtggttgg
     3121 tgctacgctt tcttccctgt cgaaccagtt gaataatatg atttatcagg cgctgggctc
     3181 ttcgttcctg acttttgact ttaactaccg ttacgacgat ttggtgtcac aaggcctggg
     3241 agaatggcag ttggccatga gcagccaact gctggacaac aggttaatca ttaatggcaa
     3301 tattggatcc cgcgaagacc tggttagtaa taatactcaa tttattggcg attttgactt
     3361 ggagtataaa ttctctcaat ccggacgctg gaggctaaaa atgttcaacc gctccaacga
     3421 caatcgttac tttaaatcgg ccatgactac tcagggcgtg ggcttgggat atagggaaag
     3481 cttcaataat ctactggagc taagacagtt gtttgctgag cgcattgccg atcagatcat
     3541 taaaagcctg aaagaatccg acttgaagaa ataattttca ataagcccgg gcaaaaatta
     3601 cccggcgggc cgaaggctta ccgctgaaaa tacaggtacc cggagtctga tccccatcca
     3661 aaggaatgca gcgtatagtt gctttggttt cgttcttaac cctttcctcg gtctccggac
     3721 tgccatccca atgacaagat aaaaagcctc cttcttcgat tttctccttg aattcatcgt
     3781 aggaattcac ttcaaaagta tttttgctgc gatagtccaa ggcttttcgg tatatgttct
     3841 gctggatatc ttcgagtagt tcttttacat aaagtccaat accttccatc ggacgggttt
     3901 ccttgctcaa agtatcacgg cgggcaactt ctatggtatt attctgtaaa tctcttgctc
     3961 ccagagccaa acgcacggga acccctttga gttcgtattc cgaaaatttc cagccgggtt
     4021 ttttattgtc gttgttatcg taacgaacac taatgcctaa atcctttaat atcaacatta
     4081 tattggacac cttctcatct atttctttaa gctgttcggc gttcttataa ataggaacga
     4141 ttaccacctg gaagggagcc agcgcgggtg gcaataccag gccgttatca tctgaatgag
     4201 acataatcag agctcccatc aaacgggtcg aaacgcccca ggaagtggcc caaacgtatt
     4261 cgagtttgtt ttctttattt aggaaagtga cgtcaaaagc tttggcgaag ttctgtccca
     4321 aaaagtgtga ggtacccgat tgcaaagctt tgccgtcttg catcagggct tcgatggtgt
     4381 aggtttccag agcacccgca aaacgttcat ttgccgtttt tacccctttt agcaccggca
     4441 tggccatata tttctcggca aaatcagcgt ataggttcaa cattttgatg gcttcttctt
     4501 cggcttcctg tttggtggcg tgagcggtat gcccttcctg ccacaaaaat tcggcggtgc
     4561 gcaaaaacaa cctggtacgc atttcccagc gaaccacatt ggcccattga ttgcataata
     4621 taggcaaatc tctgtaggat tggatccagt ctttataggt gctccagatt atggtttcgg
     4681 aagtaggcct tacaaccaat tcttcttcga gtttggcttc gggatctact atcagttggt
     4741 ttccgtcggg agattttttg agcctgtaat gcgttactac tgcgcattct ttggcaaagc
     4801 cttctacatg ttcggcttcg cggctcaaaa atgatttggg aataaacaaa gggaaatagg
     4861 cattttggtg tccggtttcc ttgaacattt tgtccaattg agcctgcatt ttttcccaaa
     4921 tggaatatcc atagggctta atgaccatac aacccctgac ggcagaattt tctgccaaat
     4981 cggctttaat gaccaaatca gtataccatt gtgagtaatt ctcgctgcgg gagcacagtt
     5041 ctttcaattc cttcgccatt gtatttttct ctaaaattta ttgaaactgc aaagctacta
     5101 aaaaagattt gtttgagatt tacaataaat acttaaatta gcggctctta ttgaaagagg
     5161 aaattgaaac taaagagtta tgcttgaatc cttgtccgcc cttgatcagg tgaccataaa
     5221 cttttcttct gcaggaaccc acctggtaaa cagtatttta gccttaatta tgtttggagt
     5281 tgctctggat attcgtctgg gcgttttcaa agaagtgctg gtaaaaccta aagcgctgat
     5341 aacggggctg ctttcccagc ttattatgtt acctgccgtc acttttttac tcattattgt
     5401 ttttcataaa tggctcactc ctactgtctg tatgggaatg attttggttt cggcttgtcc
     5461 gggaggcaat atttccaact ttatgaccaa tctggccaga ggcaaaacgg agctgtcggt
     5521 cactatgaca gccatcaaca ccgccctcgc tatttttact acgcctttga tttataaaat
     5581 atacggtgga ttatacgttg attacatgtc gagggcttcc ggcaatttgt tgcaacccat
     5641 agtcatccca ttgggccaaa tgttctacac tatttttatt cttctgggaa ttcctttaac
     5701 tctgggtttg ctgtgtaccc atttctttcc gaaattctca caaaaaatat ctccaggatt
     5761 tcgcagagtt tctatggctc tctttctgtt gattgtagta atggcttttt ccaacaattt
     5821 cagtctgttt ttaaaacata tacaatggat atttattatt gtccttgttc acaacggatt
     5881 agctttattg accggttttt cgttttcctc tttactaggg gtaaatcgcg gagagcggag
     5941 agcaataacc attgaaacag gtattcagaa ttcaggtttg ggattggcct tgctatttaa
     6001 tcctaaaata tttcctcccg aaatggctat aggcggtatg cttttcatta cggcctggtg
     6061 gggtatctgg catatagtat cgggcctgag cctttcctac ctatggtcaa aaaagccctt
     6121 agattaagca aataccttta atagttcttt tgttgtgttt tttttcagtt caaaaaaaac
     6181 gattgaacgc agttctttgt tctatatatt attgcgtttt tatacccgca tttgttttcg
     6241 ttgtttttac agaaacatct actataccgg ttgggaaaac attcccaaaa atgggccggt
     6301 aatatttgct cccaatcatc agaatgcctt aatggacgca cttgctgtgc tgctttggca
     6361 aaaaaagccc gttgtttttt tggccagggc cgacatgttt aaaaagaaat tcattggcca
     6421 tctgttacgc ctcgttaaga taatgcctgt ttataggatg aaagacggct attccaaatt
     6481 gaccaaaaac gaacagcaat tcaaagaggc tagttgtgta cttattaaca acggctactt
     6541 atgcctgatg cctgaaggcg gccaggacgg acatcgtcgc ctcagacctt tggtaaaagg
     6601 catattccgg attgctttcg aagcccaact ggctgtgggt aaaagcagag acgtacagat
     6661 tgtgcctgtg ggaattgact attccgaata cgaacatgcc ggttccgatc tggtggtaca
     6721 ttttggtaaa actattttgg tgtcggatta cataggtttt tacgaacaga atccggtcct
     6781 tggcatgaat actttgcgag accatttagc cggtaaaatg aaaagcctga taatcgatat
     6841 tcgaaataca gaatattacg aagcaatata tggtctgtcc cgcttgctaa gtccggctta
     6901 cattcgggcc ggagtcttga aagactcggc cattaaccgt tttaaagctc gtcgtcgctt
     6961 aagcggcagg ctcgaaaaat ttttcgaaaa tccggaaaaa caaaa
//
LOCUS       DAOSGL010000091         6950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_9464_length_6950_cov_3.768238, whole genome shotgun sequence.
ACCESSION   DAOSGL010000091 DAOSGL010000000
VERSION     DAOSGL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9464_length_6950_cov_3.768238"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKY68_04120"
     CDS             <1..327
                     /locus_tag="PKY68_04120"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOH24255.1"
                     /translation="PLNRLDAASLAQVLRYMALESPQVGLFSGSLPQAALEGTVADFG
                     KTLPGTLRMKSGSMKSVTNYAGYWRLGDKTYVVVLLCNQHKQSAARIRRGMEQLLRTI
                     YNKGLK"
     gene            324..1145
                     /locus_tag="PKY68_04125"
     CDS             324..1145
                     /locus_tag="PKY68_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24256.1"
                     /translation="MNKEELFKELKELNLLWSGLSRIPNFPKELLELSLNKLENINRL
                     VKDLEETSYQSQVKPAFEKETHTREATPAPLAADLKSKPSVAEETKPASAPVSSTPTP
                     ASSSPAQSSPTSAPVSPTAEQSSPLTTSASPLSTRESSLPTQESSLPAKESGQTEKPV
                     SPPVGKMPKLQTEKPESLVDRYEKTSSKESLQKKLESRHFADLSKALSLNDRFRFRRD
                     LFGDSPETMEQVLQDINTMQSMDEALSYLHKNYSQHSETESFADFCNLLNNHFSS"
     gene            1160..1876
                     /gene="rsmI"
                     /locus_tag="PKY68_04130"
     CDS             1160..1876
                     /gene="rsmI"
                     /locus_tag="PKY68_04130"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOH24257.1"
                     /translation="MSKLSIVPTPLGNLEDITLRALRILKEADLILAEDTRTTSKLLK
                     HYDIGTPMQSHHKFNEHKTVEQVVARIQAGEHIALVSDAGTPGISDPGFLLVRQCLQA
                     GLQVECLPGATAFVPALVVSGLPEERFHFEGFLPQKKGRLTRLRYLAELTHTFVLYES
                     PYRVLKTLTQLAEVCGPERRASVSREISKIYEETLRGSLDELIRHFSLTEARGEFVLV
                     VAGKNESREQSSLEKDHPRE"
     gene            complement(1895..4063)
                     /locus_tag="PKY68_04135"
     CDS             complement(1895..4063)
                     /locus_tag="PKY68_04135"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH24258.1"
                     /translation="MEFYNNWYVPENMALILVGNIDPQAARPLIEKSFGALPAKTLPS
                     LPEFSDWEVEGRKDFKANLAYIPRIHIGFKGAGITNPDALVIDLFTNLLSNRSRTGYF
                     DKLVLDGDLMGASASHISFEDQGRIVISAIPSYDPSQQVFASTKAVEKLLFQEIERIK
                     QGNIDAKLVENIKKAMIRSYQLNLESNSDKAEILTRIFISGEALDEALDYTGRLNKIQ
                     ITDIQRVASDYCNNNYMLFDIGTGKFPAAEKLEKPDIKPITQSSEKKSAYALAFEDLS
                     YKKQSLPVKDFEEIIIKPINTRSKLFYTQNTENDVFSLILKYGIGTEKMPYLEYSTTL
                     MNYAGIMGGYDPQEFKKAITELNVTCRYSVDEDYLYVVMEGFEEHLVEALGLLTRQIL
                     MPQLDEKQYRNVQGSIYQSRIREGKDPQIIAEALAEYMAYGDKSGYLDRPTLYNVFTE
                     MGLSKLTGEFQRATDYEAEVHFCGTMPFDEAYDILSQYLPLKANEKESSSPEWKAYKE
                     YSENTIYFLPRSDTKQAKIYFFAPGNVYKPENDLKYKAFNRYFGAGGLTSLIIEEIRE
                     KNSMAYTAYGFLATPPLAEKQVCFQGYVGTQSDKVNDAVALYMKLVDDMPLYGEREKN
                     LKAYLELEPFTTQPDFRSKSQQYEEWKRLGYTANPALQQEAEIQQFNLQQMLEFYQQE
                     LKGKPMAIAIVGDPKLIDLKALGAYGKVVKISSTKLFSKD"
     gene            complement(4147..4818)
                     /locus_tag="PKY68_04140"
     CDS             complement(4147..4818)
                     /locus_tag="PKY68_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH24259.1"
                     /translation="MKKSNLRSFLRFFTAGFFLTFAVTAVAQNGPVGSVSHFKLGNGM
                     QVYIWPDSNQTDVHGRVSVHVGSKNDPEQFTGLAHYLEHLMFKGTDRIGTTDWEQEKI
                     LYEQIIAKYDEKAASSDPKTKEALNKEINELSIKASEFSQSNEFSSLVATMGGTGMNA
                     GTSYDLTTYYNDFPPNQLERFLMLYAERFRAPVFRTFQSELETVYEEYNLYEDQLEQE
                     NFIIF"
     gene            4957..6192
                     /gene="hflX"
                     /locus_tag="PKY68_04145"
     CDS             4957..6192
                     /gene="hflX"
                     /locus_tag="PKY68_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOH24260.1"
                     /translation="MQPNFTFDTFSERSVLVGLITPEQNEQKATEYLDELAFLAQTAG
                     ATPVKRFLQRLDQPNPKTLVGSGKLLEIKEYVENNEITMVIFDDDLSAKQLKNLDQVL
                     KVKILDRTSLILTIFAERAQTAYAKTQVELAQYQYFLPRLTRLWTHLERQHGGGGVKL
                     RGPGETQIETDRRIVLNKIAQLKKELKQIDKQKVVQRSNRGKMVRVALVGYTNVGKST
                     LMNLLSKTELLVENKLFATLDTTVRKVIIENLPFLLSDTVGFIRKLPHDLVESFKSTL
                     DEVREADLLIHVVDISHPGFEEQIQVVENTLTELGAHNKPVILIFNKIDAYPFVPKEE
                     SDLSPELPENRPLQSLQASWMSKMQDNCLFISAAQRTNIDRLKQLLYDKVKEIHVTRF
                     PYNDFLYPHYDIPEKDSPL"
     gene            6205..>6950
                     /locus_tag="PKY68_04150"
     CDS             6205..>6950
                     /locus_tag="PKY68_04150"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOH24261.1"
                     /translation="MIPPYRKLNPSEIEQLNFQACTADDWDKILVHEDFSAEAIRSVH
                     FSGDIKLGKFEACHNLPGNVKKKSGIRRAHLHNCEVGNNVLIENIGEYIANYIIEDDC
                     CIEQLTFMICDAENSFGNGVEIEVLDETGGRMSRMYNGISAHTAYLMAMYPGRSDLQA
                     QLKKLVEQKALQERSAMGRVGRSSYLINAGMILNVNMGPFTKVRGSSFLYNGSINSTE
                     EAPIQVGAEVNAQSFILSPGSEIKDGVHLVR"
BASE COUNT         1844 a         1566 c         1627 g         1913 t
ORIGIN      
        1 cccctgaaca ggctggatgc ggcaagtctc gcccaagtgt tacgctatat ggccctcgaa
       61 agcccccagg tcggtctgtt ttccggcagt ctgcctcagg ccgccttgga aggcacggtg
      121 gccgactttg ggaaaacctt gccgggaaca ctacgcatga aaagcggatc gatgaaatcg
      181 gtaacgaatt acgccggtta ttggcggctg ggagataaaa cctatgtggt ggttctttta
      241 tgcaatcagc acaaacaatc tgccgcccgc atacgcagag gtatggagca actattaaga
      301 accatttaca ataagggatt aaaatgaata aggaagaact ttttaaggaa ctgaaagaat
      361 taaatttatt gtggtcgggt ttgagccgga ttcccaactt tcccaaggaa ttgctggagc
      421 tttcgctgaa caaactcgaa aacatcaacc gtctggtaaa ggaccttgag gagacgagct
      481 atcagtcaca ggtaaaaccc gctttcgaga aggaaacgca tacccgggaa gctactcccg
      541 ccccccttgc agccgatttg aaatcaaagc cctcagtagc agaggagacg aagccggcat
      601 ccgctccggt aagttcgacc ccaacacccg caagctcatc ccctgcacag tcaagcccga
      661 cttccgctcc ggtaagcccg accgccgagc agtcgagccc gctcacgaca tctgcaagtc
      721 cgctatcaac acgggaaagt tcgctcccaa cgcaggaaag ttcgctcccc gcaaaggaaa
      781 gcgggcagac cgaaaagcct gtttcgcccc cggtcgggaa gatgcccaaa ctgcaaaccg
      841 aaaaacccga aagcctggtg gatcgttatg aaaaaaccag ttccaaagaa tccttgcaaa
      901 agaaattgga aagcagacat ttcgccgatt tatccaaagc cctgagccta aacgatcgtt
      961 tccgtttcag gcgcgacctc tttggggatt ctcccgagac tatggaacag gtattgcaag
     1021 acattaatac catgcaaagt atggacgagg cgctctctta tctacacaaa aattattctc
     1081 aacacagtga aacggagagt tttgccgatt tttgtaacct gctgaacaat catttctcca
     1141 gctaagaagc acagcagcca tgtcaaaact cagcatagtg cccacgcccc tgggaaacct
     1201 ggaagatatt acgcttagag cgctaagaat acttaaggaa gccgatttga ttctggccga
     1261 agacacccgt accacttcca aactactgaa acattacgac atcggcacgc ccatgcagtc
     1321 gcatcataaa tttaacgagc ataaaaccgt tgagcaggtg gtggcgcgca tacaggccgg
     1381 agaacacatt gctttggtct ccgacgccgg cacacccgga atttccgatc cgggtttttt
     1441 gttggtgagg caatgcttgc aagccggctt gcaggtagaa tgcctgccgg gagctactgc
     1501 ttttgtgcct gctctggtgg tatcgggttt gcccgaggaa cgttttcatt ttgagggctt
     1561 tttgccgcag aaaaaaggcc gccttacccg tttacgctat ttggcggaac tgacgcatac
     1621 ttttgtgttg tatgaatctc cctacagagt gctcaaaaca ctgacgcaac tggccgaagt
     1681 atgcggcccg gagcgcaggg cttccgtcag ccgtgaaatc agcaaaatct atgaagaaac
     1741 cttgcgggga agcctggatg aacttatccg tcacttcagc ctgacagaag cccgggggga
     1801 atttgtgttg gtcgtggcgg gaaaaaacga atcccgggaa cagtcttctt tggagaaaga
     1861 ccacccccgg gaataaactt atttttcgga aatattaatc tttcgagaaa agtttggtcg
     1921 aagatatttt caccactttc ccgtatgcgc ccagagcttt caggtcgatg agcttgggat
     1981 ctcccacgat ggctatggcc atgggtttac ctttcagttc ctgttgatag aattccaaca
     2041 tttgttgcag gttgaattgt tgaatttccg cttcctgctg taaagcggga ttggccgtat
     2101 aacccaggcg tttccattct tcgtattgct gacttttact tctgaagtcg ggttgggtgg
     2161 taaaaggttc caattccagg taagctttca aattcttttc tctttcccca tacaagggca
     2221 tgtcgtcgac caatttcata tacagggcaa cggcatcgtt taccttgtcc gattgtgttc
     2281 caacataacc ttggaaacaa acttgctttt cggctagagg gggagttgct aaaaatccgt
     2341 aggcagtgta ggccatagaa tttttttccc gaatttcttc aatgatcaaa ctggtcagcc
     2401 ctcccgctcc gaaatagcga ttgaaggctt tatatttaag gtcgttttcg ggtttgtaaa
     2461 cattgccggg agcgaagaaa tatattttag cttgtttggt gtcgctacga ggtagaaagt
     2521 aaatggtgtt ttccgaatat tccttgtaag ctttccattc gggagagctg ctttctttct
     2581 cgttggcttt tagaggcaag tattgactca agatatcgta agcttcgtca aagggcattg
     2641 tgccgcaaaa atgcacttct gcttcgtagt cggttgcccg ttggaattca ccggtaagtt
     2701 tactcagtcc catttcggtg aatacattgt acaatgtggg tctgtcaaga tagccggatt
     2761 tgtcgccgta agccatgtat tcagccaggg cttcggcaat gatctgagga tctttgcctt
     2821 cacgtatacg gctttggtag atacttccct ggacgttgcg atattgtttt tcgtccagtt
     2881 gaggcatcag aatctgacgg gtcagtaaac caagtgcttc gaccaagtgc tcttcgaagc
     2941 cttccattac cacatagaga taatcttcgt cgacgctata acgacaggtg acattcagtt
     3001 cggtaatggc ttttttgaac tcctggggat catacccgcc cattatgccc gcataattca
     3061 tcagggtggt agagtattcg agatagggca ttttttcagt accgattccg tatttcagaa
     3121 ttaaagagaa aacgtcgttt tcggtgtttt gggtataaaa cagtttggac ctggtattga
     3181 ttggtttgat gataatttct tcgaaatctt ttacaggcag gctttgtttt ttgtagctca
     3241 gatcttcaaa agccagggca taagccgatt tcttttcgga actctgagtt ataggcttga
     3301 tgtcgggttt ttccaatttt tctgctgccg gaaattttcc ggtaccgata tcgaagagca
     3361 tataattgtt gttacaataa tcggaagcta ctctttgaat atcagttatt tgaattttat
     3421 taagtcttcc tgtataatcc aatgcctcgt ccagtgcctc gccggaaata aaaatccggg
     3481 tcagaatttc ggctttgtcg ctgttgctct ccaggttgag ttggtagctg cggatcatag
     3541 cctttttgat attttccacg agcttggcat caatgttgcc ttgcttgatg cgttcaattt
     3601 cctggaaaag aagtttttcc accgctttgg tgctggcaaa aacttgttga ctgggatcgt
     3661 acgaaggaat ggcggaaatt acgatacgtc cctggtcttc gaagctgata tggctggcgg
     3721 aagctcccat caaatccccg tcgagcacca gtttgtcgaa gtagccggtt ctactccggt
     3781 tcgaaagtaa attcgtaaac agatcgatta ccagtgcatc gggatttgtt atcccggcgc
     3841 ctttaaaacc aatgtgaatg cggggaatat aagccaggtt ggctttaaaa tcttttcttc
     3901 cttctacttc ccaatcggaa aattccggca gtgaggggag ggttttggcg ggcagggccc
     3961 caaagctttt ttcgatcagg ggtcgggcgg cttgagggtc gatatttccg accaaaatca
     4021 gcgccatatt ttcgggcaca taccaattgt tgtaaaattc aatcagtttg cttatttgcg
     4081 gatttttgag gtgttccgac agaccgatga tcggcctttc gtagggatgt cccttaaaaa
     4141 gattattcag aagataatga aattctcttg ttcaagttgg tcttcgtaaa gattgtattc
     4201 ttcgtaaacg gtttccagtt ccgactgaaa agttctgaat acaggagccc ggaagcgttc
     4261 cgcatagagc atcaaaaaac gttccaattg gttgggtgga aaatcgttgt aataagttgt
     4321 taagtcgtaa gatgttcctg cgttcatacc ggttccaccc atggtggcga ccagggatga
     4381 aaactcattc gactggctaa attcggaagc tttaatactc aattcattga tttccttatt
     4441 caatgcttct tttgttttcg ggtcgctgct ggcagctttt tcgtcgtatt tggcgatgat
     4501 ttgttcatag agtatttttt cttgttccca gtcggtcgta ccaattcggt cggttccctt
     4561 aaacatcagg tgttccagat agtgagccag gccggtaaat tgttcggggt cgtttttgct
     4621 gcccacgtgc acgcttaccc tgccgtgtac gtcggtctgg ttgctgtcgg gccagatata
     4681 cacctgcatg ccgttgccca gcttaaaatg cgatacagag ccgaccgggc cgttttgagc
     4741 aacagcggta acggcaaaag tcagaaaaaa acctgcggtg aaaaaacgca ggaatgaacg
     4801 taaattagat tttttcatat gcttgttgtt agaataagtc ataaaggttt ttagttttta
     4861 gttttgtaat aatgctgcat aataatactg caaaggaaac actttttttt gtttttgctc
     4921 tcacttactc ctatctttgt gcttcaaaat tgatgcatgc agccgaattt cacttttgat
     4981 accttttccg aaagaagcgt tttggtgggt ttaattaccc ctgaacaaaa cgagcagaaa
     5041 gctaccgaat atttggatga attggctttt ctggcccaaa cggctggagc aacacctgtt
     5101 aagagatttc tgcaacgatt ggatcagcct aatcccaaaa ccctggtagg atcggggaaa
     5161 ctgttggaga tcaaggagta tgtcgaaaac aacgagatta ccatggttat ttttgacgat
     5221 gaccttagtg ccaaacaact taaaaatctg gatcaggtac tgaaagtgaa aattttggac
     5281 aggaccagcc tgattctaac tatttttgct gaacgtgccc agactgctta tgccaagacg
     5341 caggttgaat tagctcaata tcagtatttt ttacctcgtc tcacccgctt atggactcac
     5401 ctggaacgtc aacatggcgg aggtggggtg aaactgcgcg gtcccggtga aacacaaatc
     5461 gagacagacc gaagaatagt ccttaataaa attgcacaac taaaaaaaga actaaagcaa
     5521 atcgataaac aaaaagtagt acaacgcagc aaccggggta aaatggttcg tgtagctttg
     5581 gttggttata ccaacgttgg taaatcaact ctgatgaatt tattgagcaa aaccgaattg
     5641 ctggtcgaaa ataaattatt tgccactctc gataccacag tgcgtaaggt tattatcgag
     5701 aatcttcctt ttttgttgag tgataccgtg ggttttatcc gaaaactgcc gcacgatctg
     5761 gtagaatctt ttaaatcgac cctggacgaa gtccgcgaag cggatttact gattcatgtg
     5821 gtggatattt cccatcccgg cttcgaagaa cagattcagg tagtagaaaa cacactgaca
     5881 gaattgggcg cgcacaacaa acctgtcatt ttgattttca acaaaatcga tgcctatcca
     5941 tttgttccta aggaagagag cgatttaagt ccggaattgc ccgagaacag gccattacag
     6001 tctttgcaag cctcctggat gtccaaaatg caggacaact gcctctttat ttccgcagcg
     6061 caacgtacga atattgatcg tctgaaacaa ctgctctacg ataaagtaaa agaaattcac
     6121 gttacccgtt ttccctacaa cgattttttg tatccccatt acgatattcc tgaaaaagac
     6181 agtccacttt aatacttatc tgttatgata ccaccttatc gcaaactaaa tccatccgaa
     6241 attgaacaac tcaattttca ggcctgtacg gcagatgatt gggacaaaat tctggtacac
     6301 gaggattttt cggccgaagc catacgcagc gtacatttct cgggcgatat taagctggga
     6361 aaattcgaag cttgtcataa cctgcccggc aatgtaaaaa agaaatccgg tattcgccgg
     6421 gctcacctgc acaattgcga ggttggcaat aatgtgctca tcgaaaacat cggagaatac
     6481 attgccaatt acatcatcga agacgactgc tgcatcgaac agcttacttt tatgatttgt
     6541 gatgccgaaa attcttttgg caacggtgta gaaatcgaag tgttggacga aaccggcggc
     6601 cgtatgtcgc ggatgtacaa cggcatttca gctcacactg cttacctgat ggccatgtat
     6661 cccggacgca gcgatctgca ggcacaactc aaaaagctgg tcgagcagaa agccttgcag
     6721 gaacgttctg ccatgggcag agtagggcgt tcatcctacc tgatcaatgc cggtatgata
     6781 ctgaacgtca acatggggcc ttttaccaaa gtcaggggtt cttctttttt atacaacggc
     6841 agcattaaca gcacagaaga agctccgata caggttgggg ccgaagtaaa tgcccaaagc
     6901 ttcattctct cgccgggttc ggaaattaag gatggggtgc atttagttcg
//
LOCUS       DAOSGL010000092         6561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_10247_length_6561_cov_4.016293, whole genome shotgun sequence.
ACCESSION   DAOSGL010000092 DAOSGL010000000
VERSION     DAOSGL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10247_length_6561_cov_4.016293"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1317
                     /locus_tag="PKY68_04155"
     CDS             70..1317
                     /locus_tag="PKY68_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOH24262.1"
                     /translation="MAQEIEIDPNTQWDMENYEGLNYRTAVLKQTILYPQQSGEIEIE
                     SGSFDLIFRIRNTNQRMRSLFDDFFDSYQEVRKTILSPKLRIQVEPFPFGKPANFNPF
                     SGNLRLSSSINTTQVKADEAVTIKLELSGEGNMKMLRIPELKFPADFDVYDPKVTNNF
                     KTTTRGVSGTKTIEYLVIPRYSGSFTIPPVNISYFDLTDKSYKILSTDEFQLEVGRSE
                     QNKEGVVAGTVVSREQLRLLGQDVRYIKVDNIRLRPRTNLIYGKTAFWLAYILPFFFF
                     LLMLYIYRKQAKANADLAAVRTRKANKVAVKRLKKASFYLKNQDKEQFLDEVLRALWG
                     YTSDKLNIPVSQLNKDNIEAQLNRRKVDAEVCAEFLQILQACEFERYAPVTEPQHMGD
                     LYTRTLEVINQMENTINNVHVKK"
     gene            1304..2089
                     /locus_tag="PKY68_04160"
     CDS             1304..2089
                     /locus_tag="PKY68_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH24263.1"
                     /translation="MLRNSLFSLLVFCLFGLQAWAKTTAYTDITEADSVLQLAASAYS
                     ESRFDDAAVLYEQVLANYGISAKVYYNLGNAYYKSGSVAPAILNYERALLLNPGDPDV
                     RFNLEMARSRVVDKIEPLGEFLLVRWYNSLGNLLDSNSWAYISLGLFFLFIVSLFVYF
                     FTRKSSLKKSSFFVGILAIFFSIFALLYSARQRDAIIHPDRAIVFSESVTVKSSPDQS
                     GTDLFLLHEGTSLRIKSKLGDWCEIELEDGNVGWIEFKHIEII"
     gene            2101..2805
                     /locus_tag="PKY68_04165"
     CDS             2101..2805
                     /locus_tag="PKY68_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH24264.1"
                     /translation="MNFWTGLLEWDVQALLSINALHNPWMDRFAWLLSETMVWSPAVL
                     VLLYVLIKNKKVETIWIVLAVALLFVFTDQVAGSVIKPLVGRLRPTRNPELLPYLDVV
                     NAYLGGTYGFPSNHAANAFAFAGFSALLFKNKVYSLSIFFWAAMVAFSRIYLGVHYPL
                     DVIVGALFGFLSAWMFYALYVWCMRRFFSWNLGTLYRPTGYTPSLYAIKDIQLLLLVL
                     LTILISLVISSYYITW"
     gene            2833..3084
                     /locus_tag="PKY68_04170"
     CDS             2833..3084
                     /locus_tag="PKY68_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOH24265.1"
                     /translation="MARIITFNELRKIKDSLPSGSTQAIADRMGLSVETVRNYFGGQN
                     YRDGKSCGIHVEPGPDGGIVKLDDTTILDMALEIINADS"
     gene            3494..4081
                     /locus_tag="PKY68_04175"
     CDS             3494..4081
                     /locus_tag="PKY68_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24266.1"
                     /translation="MYLFDLYALSVELKSERKLIKKTSSLFFSSEEKAAQAAKEYRHH
                     YACMYDSMSTEELYCIIVEGRRLDSIYQDILCTKVYSPDGQLIDSCEVPDGESFQGRK
                     KESLFHQPGDIVEIPLGEELCLGIVLGQPPLFKEDQSLYGLSSSDDSYTLIVYPSLEV
                     DYAHAPLVFKPRYKIDEAVKQSLLLAWKKHTLQSV"
     gene            4177..>6561
                     /locus_tag="PKY68_04180"
     CDS             4177..>6561
                     /locus_tag="PKY68_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH24267.1"
                     /translation="MEQFIRKYQEQLGTLLLERLNPGLDGVFKELVIWTQRYIAEVDK
                     LISSLESETDPQKKSLLLLRQYMAFCRSMLPQLDELHLSLPSAREEQLGYDQILHQYL
                     QEQPRYQIFVQSPERFNPLTGDNWYIKIIKACKRAAYKLYILPAKTCRLFNKIVKKEI
                     FKKACDIKPWKQKVPLCRLSYRYYAYELFALYSELAIDLHRELALSCTKIRELDERLF
                     PAIEGSVGKGKDGGVFKDEWVQSIRPNLLTLKKEFKSKQTKFSLQLQQGLQQIQAEFW
                     EQAQVGGTLEFNSFKHCSWYYKRLAACRRRQFVRHSLKRKNTVYAICDDWKFSQEIDY
                     LSCNALKVNILFRQQMNENRQDLQALLSDIPRFLNESLGKIQTEERQKLKKILQQLKF
                     DAGKVLVNTIIEASLAYVNRKDLLNLIERAAKSVLKDLSEMTNKRSLIARFDPAKAYP
                     KRALKGVSTYRLIQFEIGARWNRFAVSLKQQTQKDLENIYDQLQNLGRINLINLEAAL
                     VELDDKKEHIASNVYQDARSGLERALMQYKAMLEFCDNMYIKLDQSVSKAVTDLVSAL
                     LDLAINENTEVIRLRIHRADIRKSLSKSYTFFIRILKVLYVKTRALFGLVKKGTGAGI
                     GTLKAKLGIQDQAKEITTDISEFLSFDYEDIQRLPFVYQRLFSSEPLKDDSFYLHRKK
                     ESEQLAATYQKWREGQFMPCLIYGEKGSGVSTLMEMFLPQNNEETCRLIKITPGKRIY
                     KAGDLLDLLGRSIRNKAFKDLDDLYTYIEQQAPFCIFLDKLHFMYMRQPKGFEVL"
BASE COUNT         1965 a         1258 c         1424 g         1914 t
ORIGIN      
        1 tataaattgt atacgctgat agatataagc tgagtaaata atatcaagtt tcctgaattc
       61 aaaggctttt tggcccagga aattgaaata gatcccaata ctcagtggga tatggaaaat
      121 tacgagggtc tgaattatcg aacagccgtc ttaaagcaaa ccatccttta tccacagcag
      181 agcggcgaga ttgaaattga aagcggaagt tttgacctga ttttcagaat acgaaacacc
      241 aatcagagga tgcggagttt gtttgacgac ttttttgatt cttatcagga agtacggaaa
      301 accatactat cgcctaagct ccggattcag gttgagccgt ttcctttcgg caaaccggct
      361 aacttcaatc ctttcagcgg caacttgagg ctgagctctt ccatcaacac tactcaggtt
      421 aaagccgacg aagcagtcac catcaaattg gaactcagcg gagaaggaaa tatgaagatg
      481 ctcagaatcc ctgagctgaa attcccggcc gattttgacg tgtacgatcc caaggttacc
      541 aataatttta agactactac ccggggagtc agcggaacca aaaccataga atacctggta
      601 attccccgct attccggcag ctttaccatt cccccggtga atatttctta ttttgacctg
      661 accgataaat cttataaaat tttgtctacc gatgaatttc aactcgaggt gggccgttcc
      721 gaacaaaaca aagaaggggt ggtggccggt acggtagtaa gtagagaaca attgcgtttg
      781 ctgggtcagg atgttcgtta catcaaagtg gataatatcc gcttgcgtcc ccgcactaac
      841 cttatctatg gcaagactgc cttttggttg gcctacatac tgcctttctt ctttttcctg
      901 ctgatgcttt atatttatcg taagcaggcc aaagccaatg ccgacttagc cgctgtcagg
      961 accaggaaag ccaataaagt ggccgtaaaa cgtctcaaga aagccagttt ttacttgaaa
     1021 aaccaggaca aagaacaatt cctcgatgaa gtattgagag cgctttgggg ttataccagc
     1081 gacaaactca atattccggt ttctcagttg aacaaagata acatcgaagc ccaattgaac
     1141 cggcgaaaag tggatgcaga agtctgtgct gaatttttgc agattctgca ggcatgtgaa
     1201 tttgaacgtt atgctccggt aaccgaaccc cagcatatgg gcgatttgta taccagaact
     1261 ttggaagtta tcaaccaaat ggaaaatact ataaacaatg tccatgttaa gaaatagcct
     1321 tttcagctta ttagtctttt gcttgtttgg cttgcaggct tgggctaaaa cgacagctta
     1381 tacagatatt accgaggccg actctgtgct gcagttggct gccagtgctt attccgaaag
     1441 ccgttttgac gacgcggcag tattgtacga acaggtattg gcaaactacg gtatttcggc
     1501 caaagtatat tacaacctgg ggaatgccta ttataaatcg gggtctgtgg caccggccat
     1561 tttgaattat gaaagagctt tgctgctcaa tccgggagac cccgatgtgc gatttaatct
     1621 ggagatggcc cgcagtcgtg tggtggacaa aatagagccc ttaggagagt ttttgttggt
     1681 acgatggtac aattcgttgg gcaatctgct cgattccaac tcctgggcct atataagcct
     1741 gggcttgttt ttccttttca tagtcagttt gttcgtttat ttctttacca gaaaaagcag
     1801 cctcaagaaa agcagctttt ttgttggaat acttgcaatc ttttttagta tatttgcttt
     1861 gctctattcc gcccggcaac gtgacgctat tattcatccg gacagggcca ttgtcttttc
     1921 ggagagtgtc acggtgaaaa gctctccgga tcagagtggg accgatcttt ttcttttaca
     1981 cgaaggcact tctcttagaa tcaaaagtaa attaggcgat tggtgtgaaa tagaacttga
     2041 agacggaaat gtaggctgga tagaatttaa acacattgag attatttaga cttaaacagc
     2101 atgaattttt ggaccgggct tcttgagtgg gatgtacagg ctttgttgag tatcaatgct
     2161 ctgcacaatc catggatgga tcgctttgcc tggttgctgt cagaaaccat ggtttggtcg
     2221 ccggctgtat tggtcttgct ctatgtgttg attaaaaata aaaaagtcga aacgatctgg
     2281 atagtgttgg ccgttgccct tttgtttgtg tttactgatc aagtggcggg cagcgttata
     2341 aaacccttgg tgggaaggct taggcctacc cgcaatcccg aattgcttcc atatttggat
     2401 gttgtaaatg cttatctggg tggcacttac ggttttcctt ccaatcatgc tgctaatgcc
     2461 tttgcttttg caggtttcag tgccttgctg ttcaaaaaca aagtttatag cttgagtatt
     2521 ttcttttggg cagctatggt tgctttttcc cgtatatatc tgggtgtgca ttatccgctc
     2581 gacgtgattg tcggcgcttt gttcggtttt ttgtccgcct ggatgtttta tgccttatat
     2641 gtatggtgta tgcggcgttt cttttcgtgg aacctgggca ctttgtaccg tcccactggt
     2701 tacacaccga gtttgtacgc tataaaagat attcaactct tgctgctggt gctgttaacc
     2761 atactcatca gtttggtcat ttcatcctat tacataacct ggtaaattaa ttaatctata
     2821 aaatcttaag tcatggcgag aataatcacc ttcaatgaat tgcgcaaaat taaagatagt
     2881 ctgccttcgg gcagcactca agctatcgca gacaggatgg gcctctctgt agaaactgtt
     2941 cgtaattatt ttggcggaca aaattacaga gacggcaaaa gttgcggtat ccatgttgaa
     3001 ccagggcctg atggcggcat tgtgaagttg gacgatacta ctattttgga tatggctttg
     3061 gaaattatta atgccgacag ttaaatcctt atttcgaaac agtatttata ttaagggctg
     3121 cctcaaaata aaggcagcct tttttgtgta aaacatcggc aattggcttt ttgtttttag
     3181 tttcaagtcc aaagacagaa aaaacatgga gtgatcagcc taaatcaatg aatatttatt
     3241 catctgataa ccatttatat ataaagtcca aataaaataa tagattcttt tatttcttta
     3301 aaccttgata gacaggggat aattaataaa acattatata attaaacata gaaatgaaaa
     3361 tagttttgct taaaacacaa aaatatgtgt tatatttacg ccgaaattaa ataagagaaa
     3421 acaattttat ggaaatattc aaattgtcac gtattaaaat taatgttttt actaaccaaa
     3481 aaaggtaaaa tttatgtatc tattcgattt gtacgctcta agcgtagagt taaagtcaga
     3541 gagaaaacta atcaaaaaga cttcttcctt atttttttct tctgaggaaa aagctgctca
     3601 agcagcaaaa gaatacagac atcactatgc ttgtatgtat gattctatga gtacagagga
     3661 attgtattgc ataatcgtag agggacgcag attagacagt atttatcaag atattttgtg
     3721 tacaaaagtg tactcccctg atggccaatt gatagacagt tgcgaagtgc cggatggaga
     3781 atcctttcaa ggcagaaaaa aagaaagctt atttcatcaa ccgggcgata tcgtagaaat
     3841 tcctttagga gaggaattgt gtttggggat agtattgggg cagccgccgt tgtttaaaga
     3901 agaccaatca ctatacggcc tgagttcctc ggatgattcc tacaccttaa tagtatatcc
     3961 ctctttggaa gtggattatg cccatgcacc tcttgtattt aaaccccgtt ataagataga
     4021 cgaagctgta aaacagagtc tcttacttgc ctggaaaaag catactttac aatcagtgta
     4081 agcaggaaaa aataaaagca tttttggaat ccttgtccat attgaaatac aattaagtcg
     4141 tatatttgtt tggacgttta tagtttaaac aagtatatgg aacaatttat acgaaaatac
     4201 caggagcaac ttggaacatt gctactggag agattgaatc ctggtttgga tggggtgttt
     4261 aaggagctgg ttatttggac tcaacgctat attgccgagg tagataaact tatttcctct
     4321 ttggagtctg aaactgatcc gcaaaaaaaa tctctgttgc tgttgaggca atatatggca
     4381 ttttgccgat caatgttgcc ccaactggat gaattgcatt tatctctgcc atcggcacgt
     4441 gaggaacagc tgggctacga ccagatcttg catcaatatc ttcaggagca accaaggtat
     4501 caaatttttg tacagtctcc ggagaggttc aatcccctga ccggcgataa ttggtatatc
     4561 aaaatcatta aagcttgcaa aagagcagct tataaattgt acatacttcc agccaaaacc
     4621 tgcaggctgt tcaacaaaat tgtaaaaaaa gaaatattca aaaaagcctg cgacattaaa
     4681 ccctggaaac aaaaagtacc cttatgcagg ctgagttatc gctattacgc ttacgaatta
     4741 tttgctctct attctgaact tgccattgat ttacaccgtg agctggcttt atcttgtacc
     4801 aaaatccgtg agttggacga acgcttgttt cctgctatag agggatctgt agggaagggc
     4861 aaggatggag gagtattcaa agacgaatgg gtgcaaagta tacggcccaa cttgttgact
     4921 cttaaaaagg aattcaagag caagcaaaca aaattctcgc tacagcttca acaaggatta
     4981 caacaaattc aagctgaatt ttgggaacaa gcccaagtgg gagggactct ggaatttaat
     5041 tccttcaaac attgctcctg gtattacaaa cgcctcgcag cctgccggcg taggcagttc
     5101 gttagacata gcctcaagcg caagaatacg gtttacgcca tttgtgacga ttggaagttt
     5161 agccaggaaa ttgattattt gtcgtgcaat gccctgaaag tcaacatctt gtttaggcag
     5221 caaatgaacg aaaatcgtca agacttacag gctttattat cggatattcc ccgtttttta
     5281 aacgagagtc ttggaaagat acaaaccgaa gaacgtcaaa aactgaaaaa aatcctgcag
     5341 caacttaaat tcgatgcagg taaagtactg gtcaatacca tcattgaagc ctcacttgca
     5401 tacgtcaatc ggaaagattt attgaacctg atagaacgtg ctgccaaatc tgttttaaaa
     5461 gacttgagcg agatgacgaa caagcgtagc ctgattgcac gttttgatcc ggcaaaagcc
     5521 tacccgaaga gagctttgaa gggtgtatcc acataccgcc ttattcaatt tgaaattgga
     5581 gcaagatgga atcgtttcgc agtgtcgtta aaacaacaaa cgcaaaaaga tctggagaat
     5641 atttatgacc aattgcaaaa tttgggaagg atcaatttga tcaacctcga agctgctttg
     5701 gttgaactcg acgacaaaaa agaacatatt gcttcgaatg tttatcagga tgcccgttcg
     5761 ggcctggaac gggccttaat gcaatacaaa gccatgttgg agttttgcga taacatgtat
     5821 ataaagctgg atcagagcgt gagtaaagcc gtgacggatt tagtctctgc tttgctcgat
     5881 ttggccatta acgaaaatac tgaagtgatt cgcttacgca tacacagggc cgatattagg
     5941 aaaagtttgt ccaaaagtta tacttttttc ataaggatac tgaaagttct ctacgtaaaa
     6001 accagagctt tatttggttt ggtaaaaaaa gggacaggag caggcatagg cactcttaaa
     6061 gctaaattgg gcatacagga tcaagccaaa gaaattacaa ctgatatatc ggaattttta
     6121 tcgtttgatt acgaggatat tcagcgacta cctttcgttt atcaacggct tttcagcagc
     6181 gaacctctca aggacgacag tttttacttg catcggaaaa aagaaagtga acaacttgcc
     6241 gcgacttacc aaaaatggcg cgaaggccag tttatgccct gtctgatata tggagaaaaa
     6301 gggagtggag tgagtactct gatggaaatg tttcttcctc aaaataacga ggaaacttgc
     6361 aggctcatta aaatcacacc cggcaagagg atatataaag ccggggattt attggactta
     6421 ttgggtcgct caatccgtaa caaggcattt aaagatttgg acgatctgta tacgtatatt
     6481 gagcagcaag ctcccttttg tatattcctc gacaagttgc attttatgta tatgcgtcaa
     6541 cctaagggct tcgaagtgct c
//
LOCUS       DAOSGL010000093         6476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_10441_length_6476_cov_4.695998, whole genome shotgun sequence.
ACCESSION   DAOSGL010000093 DAOSGL010000000
VERSION     DAOSGL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10441_length_6476_cov_4.695998"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="gldM"
                     /locus_tag="PKY68_04185"
     CDS             complement(<1..1206)
                     /gene="gldM"
                     /locus_tag="PKY68_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOH24268.1"
                     /translation="MPPSNAPETTRQRMIGLLYIMLLCMLALNVSSDVLGGFELVEDS
                     LLKSTHNSSIQNKSLYDDLYASYLQNPEKSGEWYQRAQEVKQSADSMYNYVEEIKWEI
                     VRKADGKKADIHNVIKREDVNSTAFVMLPPTGKKGRELAQEMETFREKMVSMVGDTLK
                     QQLILDNFSTTPSQKDRIQGKSWETAMFDNMPVSAVITMLSKLQNDIRHAEGEVLYTL
                     TKNIDVGDFRVNRIKAYVIPNSQNIVRGGSYRANIVLSAEDSTQRPQVYINGEALPSS
                     QNGLYEVYANRIGTFPVAGHIELQHGDGSKRRYEFSEEFTVVEPTATVSNIMMNVLYA
                     GIENELSISVPGVASQDISASVNNGRLSRAGNVWRALPTEVGKECIVSVNAVMDGRSQ
                     QVARVPFRVR"
     gene            complement(1219..2391)
                     /gene="gldL"
                     /locus_tag="PKY68_04190"
     CDS             complement(1219..2391)
                     /gene="gldL"
                     /locus_tag="PKY68_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOH24269.1"
                     /translation="MSDKQQKQREESRLAEFLSSYRGKTILNFFYGFGASLAILGTLF
                     KLLHLDGANTMLAIGLGTEVLIFAISAFDPPFRNYRWEEVFPVLKTRKAEDRPDFSNL
                     GLGGGVIIHGGAPVGAGQTASVQSAGVGQTAQSQGATPHYQGQVPRPAPFADITPQQA
                     AQSFGIPPQVQLSPEDSEVLADSIKRMGEAVRQLNALANITKVTEDYLQQLSGMTENL
                     SRFSHATGSLAEVSNVLLDSYKNITENSDQITSNSKGYVEQMDALNLNLKGLNTIYGI
                     QLKSISSQIDTIDRVNKGLLHIKEMYEGSAMDSERFRSETQQMADHLASLNQFYARML
                     NAMIPSNNPMMSMAYMATGQGQQAKQDNTAEQTPETGGNSGVKDLQEEENEDNNQQ"
     gene            complement(2411..3820)
                     /locus_tag="PKY68_04195"
     CDS             complement(2411..3820)
                     /locus_tag="PKY68_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH24270.1"
                     /translation="MRKNLIIGSALILLASCSSYDNGELTGVQTSSWNEPNPYGMILI
                     RQGTFELGQSATDSIWGTELPSKKVSVGSFWMDETEITNGQYKQFVKWVCDSIIRERL
                     ADPLYGGNDEYKITENQLGDPITPYLNRKLPIPDARRATEEELSALNYLTDPDPIRGD
                     RRLVPERITYRYNWFDQEAASKRENQLILEKRERNTDVNTALLPQVMIEKDTAYFGQD
                     GRIVRETLRRPLRSMWDFVHTYIVEIYPDTTCWVNDFENSYNLPYLINYFSHPGYSAH
                     PVVGVSWEQAKAFCHWRTEYLNSALRSKGQKVLEYRLPTEAEWEYAARGGKLVTRFPW
                     EGEYTNNEKNCFYANFKTGEGDFSSDGGLIPVKVASYPANEYGLYDMAGNVAEWTSST
                     WSASSYQEMNDLNPEFKFRSSHNDPYLTKRKVVRGGSWKDPAQFIQVNSRTYEYQNET
                     RSYIGFRCVRSQIGGGGSK"
     gene            complement(3825..4799)
                     /locus_tag="PKY68_04200"
     CDS             complement(3825..4799)
                     /locus_tag="PKY68_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOH24271.1"
                     /translation="MIRLRRFFIFIVGLLIRTGAQAQFDAQWSQYMQLPGLYNPGAIG
                     LNSDLNVHLGFRQQWLGFENAPGTFSVNASMPLRAGKTLNGLGLVLTNESIGLFSTQL
                     FQIQYAYKKKLLDGELSAGLQGGILQQNFNSGGIYIPNSEYHNPNDQAIPSGDIEGII
                     PDFSLGVWYSRPEFYAGLSASHLLGSKIKLSRKESTEEDKEVLFKVSQSYYLTGGYNI
                     NLSNPLYVLQPSILAKTDFVAWSADLSCLLKYNKEYWGGLSWRPGEAIVAMVGVMFDF
                     GLVVGYAYDVPISQLIKFTSGSHELFIAYRKKIDTSLISKKQKSIRIL"
     gene            complement(4976..5197)
                     /locus_tag="PKY68_04205"
     CDS             complement(4976..5197)
                     /locus_tag="PKY68_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOH24272.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRSGAPLEVIENLQEME
                     DEGEIYESIEDLWPDYPSKEDFFFNEDEY"
     gene            complement(5341..5904)
                     /locus_tag="PKY68_04210"
     CDS             complement(5341..5904)
                     /locus_tag="PKY68_04210"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOH24273.1"
                     /translation="MKIYTRTGDTGTSSLADGERVLKNDLRLEAYGTTDELSAQLALL
                     RTLLKTEEEKNRILELQKHLFRISGRLAYRKPPENDNIRSLFQDYARLDKELTHQLEK
                     AIDCIQKKLPVTHSFVVPGGSAAAAQAHICRTVCRRAERKCIAVHQTEKLNESMLTFL
                     NRLSDYLYILSRKLCLDETDELFWDGK"
     gene            6119..>6476
                     /locus_tag="PKY68_04215"
     CDS             6119..>6476
                     /locus_tag="PKY68_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24274.1"
                     /translation="MQRIKHHIHYYLHARHRRGHGVHSPFAYRLITAIIEEDYPYYCF
                     NELEDKRVRMESERIRLETKRARLKARHAVRKAKGKGLDAESPGRKARQSRSGRHCLS
                     PADAQILFRLVNAFALH"
BASE COUNT         1497 a         1506 c         1640 g         1833 t
ORIGIN      
        1 cctgaccctg aacggaactc tggccacttg ctgactgcgt ccatccataa cggcattgac
       61 gcttactata cattctttgc ctacttcggt gggtaaagct cgccatacat ttcctgcacg
      121 cgacaggcgg ccgttgttga cgctggcgct gatgtcctgg ctggccaccc cgggaacgga
      181 gatgctcagt tcgttttcga tacctgcata cagcacattc atcatgatgt tggagactgt
      241 ggcggtgggc tcaaccaccg tgaattcttc cgaaaattcg taacgccgtt tgctgccgtc
      301 tccgtgttgc agttcgatat gtccggcaac agggaatgtt cctatgcgat tggcatacac
      361 ctcatacaat ccgttttggc tggaaggcag agcttcgccg ttgatgtaaa cctggggacg
      421 ctgggtagag tcttcggccg aaagtacaat attggctctg taactgccgc cacgtacaat
      481 gttttgtgag ttgggaatta cgtaagcttt gatgcgattc accctgaagt cgcccacgtc
      541 gatatttttg gtcagggtat acaatacctc gccttcggca tgccggatat cgttctgcaa
      601 tttggaaagc atggtgatta ccgcactgac gggcatattg tcgaacatgg cggtttccca
      661 actcttgccc tggattctat ctttttgcga aggtgtggtg gagaaattat cgagaataag
      721 ttgttgtttc agggtgtcgc caaccatgga aaccattttt tcgcggaaag tttccatttc
      781 ctgagccaac tcacggccct ttttcccggt gggaggcaac atgacaaaag cggtggagtt
      841 aacgtcttcg cgtttgatta cattgtgaat gtcggctttt ttcccgtcgg ctttgcgcac
      901 aatttcccat ttgatttctt ccacgtaatt gtacatcgag tcggcagact gtttgacttc
      961 ctgagcacgt tggtaccatt ccccgctttt ttcggggttt tgtaggtaag aagcgtagag
     1021 gtcgtcgtag agcgacttgt tctggatgct cgagttatgg gtactcttga gcaaactgtc
     1081 ttcgaccagt tcgaaaccac ccaacacgtc cgaggagaca ttgagtgcaa gcatacagag
     1141 cagcatgatg tacaacagtc cgatcattcg ctgtctggtg gtttcgggag cattgctggg
     1201 aggcatattt cttgtagatt attgttggtt gttatcttcg ttttcttctt cctgcagatc
     1261 cttaacgccg gaattgccgc cggtttcggg agtttgttcc gcagtgttgt cttgtttcgc
     1321 ctgttggccc tgaccggtgg ccatataagc catgctcatc atggggttgt tgctgggtat
     1381 catagcgttg agcattctgg cgtagaattg attaagactg gccagatgat cggccatctg
     1441 ttgggtttcg cttctgaagc gttcgctgtc catggccgac ccttcgtaca tctccttgat
     1501 gtggagcaag cctttgttca cacggtctat ggtgtcgatc tgcgaactga tgctcttgag
     1561 ttgtattccg taaatggtat tcaggccttt cagattcaga ttcagggcat ccatctgttc
     1621 cacgtaacct ttggaattgc tggtaatctg atcggagttt tcggtgatgt tcttgtatga
     1681 atcgagcaag acattggaaa cctcggccaa agaacccgta gcatggctaa agcggctgag
     1741 gttctcggtc atacccgaca attgttgcag gtaatcttcg gtcactttgg taatatttgc
     1801 caaggcattg agctgacgaa cggcttctcc catacgtttg atgctgtctg ccaatacctc
     1861 ggaatcttcg gggctgagtt gtacctgggg aggaatgcca aagctctggg cggcttgttg
     1921 gggcgtaatg tccgcaaaag gagctggtct gggaacctgc ccctgatagt gtggggtagc
     1981 gccctgactt tgggccgttt ggccgactcc ggcgctttga accgaagctg tttggccggc
     2041 tccgactgga gctcctccat gaatgattac acctccaccc aggccgagat tgctgaaatc
     2101 gggccggtct tcggcttttc ttgtttttag cacggggaat acttcttccc agcgataatt
     2161 ccggaaggga ggatcaaaag ccgaaatggc aaatatcaat acttcggtac ccaacccgat
     2221 ggccaacatg gtgttggctc cgtcgagatg caggagtttg aacaaggttc ccaggatggc
     2281 cagagaggct ccaaagccat aaaagaaatt taagatggtt tttccccgat agctgctcag
     2341 gaattccgcc aatctggatt cttctctctg tttctgttgt ttgtcggaca taatatgcga
     2401 tgtttaatta ttatttactg cctcctccgc ctatttggct gcgtacacaa cgaaatccga
     2461 tgtaggaacg ggtttcgttt tggtattcgt aggtgcgtga attgacttgt ataaattggg
     2521 cgggatcttt ccaggagcca ccgcgaacca ctttgcgttt ggttaggtag ggatcgttgt
     2581 gactgcttct aaatttgaat tccggattta gatcgttcat ttcctgatag gaagaggcag
     2641 accaggtact cgaggtccat tcagccacgt tgccggccat gtcgtataga ccgtattcgt
     2701 tggcgggata ggaggctacc ttgacgggaa ttaaaccgcc gtctgaggaa aaatcgccct
     2761 cgccggtttt aaagttggca taaaaacagt ttttttcgtt gttggtgtat tctccttccc
     2821 aggggaagcg ggtaacgagt ttgcctccgc gggcggcgta ttcccattcg gcttcggtgg
     2881 gaagacggta ctcaagcact ttttggcctt tgctcctgag cgcactgttg aggtattccg
     2941 tccgccagtg acaaaaagcc ttggcctgtt cccaggaaac accgaccaca gggtgggcgg
     3001 aatagcccgg atgtgaaaaa tagttgatca ggtaaggcaa gttgtagctg ttttcaaaat
     3061 cgttcaccca gcaggtggta tcggggtaaa tttccacgat ataggtatgt acaaaatccc
     3121 acatcgacct aagggggcgt cgtaaggttt cacgtacaat tcttccgtcc tggccgaagt
     3181 aggcggtgtc tttttcgatc atcacctggg gcaggagggc tgtgttgacg tcggtgttgc
     3241 gttcccgctt ttcgagaata agctggttct ctcttttgga ggctgcctct tggtcgaacc
     3301 agttgtagcg atatgtgatc cgttcgggta ccagcctgcg gtcgcctctg atgggatcgg
     3361 ggtctgtcag gtaattcaga gcgctcaatt cttcttcggt ggcgcgacgt gcatcaggta
     3421 tgggcagctt acggttgagg tagggggtga tggggtctcc caactggttt tcggtgattt
     3481 tgtattcatc gttacctccg tagagcggat cggccaggcg ttcacgtata atggaatcgc
     3541 agacccattt tacgaattgt ttgtattgtc cgttggtaat ttcggtttcg tccatccaga
     3601 aagatcctac cgaaactttc tttgaaggaa gctcggtccc ccatatactg tctgtggcag
     3661 attgtccaag ttcaaaagta ccttgccgga tcaaaatcat tccgtaagga ttgggctcat
     3721 tccacgacga agtctgtact cccgtcaact ccccgttgtc gtaagaactg caggaagcca
     3781 gcaagataag tgctgagccg atgatcaggt tttttctcat tgttttataa aattcgtatg
     3841 ctcttttgtt ttttactgat cagcgaggta tcgatttttt tgcggtaggc gatgaataat
     3901 tcgtggcttc cgctggtgaa tttaatcaac tggcttatgg gtacgtcgta ggcatagccc
     3961 actaccaatc caaaatcgaa catcacgcca accatagcaa ctatggcctc gccaggcctc
     4021 cagctcaagc ctccccaata ttctttgttg tatttaagca gacaggataa atccgcactc
     4081 caggcaacaa agtcggtctt ggccaaaatg gagggttgca gcacatataa cggatttgac
     4141 aggttgatat tgtatccgcc ggtcaaataa tagctttgag agactttaaa aagtacttct
     4201 ttgtcttctt cggtactttc tttgcgactg agcttaatct tgctgcccaa gaggtgcgaa
     4261 gccgacaaac cggcgtaaaa ttcgggacgg gaataccaga cacccaggct gaaatcggga
     4321 ataatgcctt cgatatcgcc cgagggaatg gcctggtcgt tggggttgtg gtattcactg
     4381 ttgggaatgt aaatgccacc cgaattgaag ttttgttgca aaattcctcc ctgcaagcct
     4441 gcgctgagtt cgccgtcgag aagtttcttt ttataagcgt attggatctg aaacagttga
     4501 gtgctgaaca aacctatact ttcgttggtt aagaccaggc ccaggccgtt gagcgttttt
     4561 cccgcacgca ggggcatact ggcattgaca ctgaaagtgc cgggtgcatt ttcaaagcca
     4621 agccattgct gcctgaagcc caaatgcacg ttcaggtcgc tgttgaggcc tatggcaccc
     4681 gggttgtata agcccggaag ttgcatatat tgactccatt gggcgtcgaa ctgagcctga
     4741 gcacccgtcc ggattagtaa gccaacgatg aatataaaga atctcctgag tctgatcatt
     4801 cgcaagaata aatcctccgg ctgtgccggt aaatcctttg gacagccagg tagtataaca
     4861 gaaaaatgcg gaaaaaattg tttggggata caaaaaaaac ctcaaacgca taaacaaaaa
     4921 atggacatag tgcatgtcca tttactttgt taagcccgag atcaatcgag attaattaat
     4981 actcgtcttc gttgaaaaag aaatcctctt tacttgggta atccggccac agatcttcta
     5041 tgctttcgta gatttcccct tcgtcttcca tttcctgaag gttttctatc acttcaaggg
     5101 gagctcccga acggatggca taatcgatga gctcatcctt ggtggcgggc cagggagcat
     5161 cttccaattt tgaggctaat tccagggtcc agtacatagc tataattatt aattgataga
     5221 cgtataatta tgaataatcc aggtcaattt ttgtgttttt acaataaggc tgcaaaagta
     5281 taagaatagt ttgaatagtc aagtaaaaaa gagcttgttt tatatcgata attaaaatag
     5341 ttatttgccg tcccaaaaaa gttcatccgt ttcatccaga caaagtttac gactgagtat
     5401 ataaagataa tccgaaagcc tgttcaggaa agtcagcatc gattcattca gtttctctgt
     5461 ttgatgcacc gcgatgcact tacgttcggc gcgccgacag acggtgcggc aaatatgagc
     5521 ctgagcagcc gccgcgcttc ctccgggaac gacaaaagaa tgggtgaccg gtaatttttt
     5581 ctgaatgcag tcgatagcct tttcaagttg atgagttagc tccttgtcca gccgggcata
     5641 atcctgaaac aacgagcgaa tgttgtcgtt ttcgggcggc ttgcgatagg ccaggcggcc
     5701 ggatatccgg aataagtgct tttgcagctc cagaatccgg tttttttctt cttcggtttt
     5761 gagcagggtt ctgagcaagg ccagttgggc gctgagttcg tcggtagtgc cgtaagcctc
     5821 cagacggaga tcgtttttca aaaccctttc tccgtctgcc aggctgctgg ttcctgtatc
     5881 tccggtgcgg gtgtaaatct tcataccaat atatcttaag ctcggcaagc atccggttta
     5941 atctttcccg ggtttgtgta aatcttcatg ccaatatatc ttgagcctgt tccggcgttt
     6001 agcttcttcc agcccttcaa actataagtt ttacaggcga cacaaaaata acacaatgca
     6061 gggaaaacgc aaattatgca gaatatattt tacctttgcg cattcatcga ttgttgtttt
     6121 gcaacgtata aaacaccata tacactacta tttacacgcg cggcaccgcc ggggtcacgg
     6181 agtgcattcc ccatttgctt accgactgat cactgctata atcgaagaag attatcctta
     6241 ttattgtttc aacgagttgg aagacaagcg agtccgcatg gaatccgaac gaatccggtt
     6301 ggaaactaag cgagcccggc tgaaagcccg gcacgcagtc cggaaagcca aagggaaagg
     6361 acttgatgcc gaaagccccg gccggaaagc ccggcagtcc cgctccggga ggcattgcct
     6421 gtctcctgcc gatgcccaaa ttttgttccg gctggtgaat gcctttgctt tgcaca
//
LOCUS       DAOSGL010000094         6445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_10513_length_6445_cov_4.678873, whole genome shotgun sequence.
ACCESSION   DAOSGL010000094 DAOSGL010000000
VERSION     DAOSGL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10513_length_6445_cov_4.678873"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PKY68_04220"
     CDS             complement(<1..487)
                     /locus_tag="PKY68_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOH24275.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGSDPVVIANSEGKRTTPSIVAFID
                     GGERKVGDPAKRQAITNPKHTVSSIKRFMGERFDNVQTDVKRMPYTVTKGENNTPRIQ
                     IEDRNYTPQEISAMVLQKMKKTAEDYLGHEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LK"
     gene            complement(689..2932)
                     /gene="rho"
                     /locus_tag="PKY68_04225"
     CDS             complement(689..2932)
                     /gene="rho"
                     /locus_tag="PKY68_04225"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOH24276.1"
                     /translation="MPNYNIVQLNAKSLQELIELAKSINIKKVDSSSKEALIYAILDE
                     QALQSASRTVAQMKENAADERRKRKRIPKNEKQDKAKESNPSDSSKKTQQDKNLLSSS
                     LPVTDLDVIQKEVLSKKASRTSADKKESAQKKDSVQKKDSPSKKETVTKKEPIVSSDV
                     ATQSEEQKPRKPGRPRKKKSDEESASGNATMSTQETSPKGKSPKTAEAAKEQEAQVRQ
                     EDKATQTKKSSAAKDAADAQKQQKPGASGYKKEDGKPAGEGGKTPTGKASSEDKPSAE
                     DKPVPKKGRAPEDTQAGALYEGSTDEELSTESTQIDTEEKPSLQTADEEALIEARRLA
                     GLLPKAQDTYNQGRQQKHQGNNAGYKSQRLPKTEEEKIFEFEGILMGSGCLEIMPENY
                     GFLRSSDYNYMSSPDDIYVSQSQIKLFGLRTGDMVLGPIRPPKEGEKYFPLVRVERIN
                     GRDPSYVRDRLSFDHLTPLFPEEKFNLTGTKAYANTSVRVVDMFSPIGKGQRGLIVSQ
                     PKTGKTVLLKDIANAIAANHPEVYMIILLIDERPEEVTDMARSVRAEVISSTFDEPAE
                     RHVKIAGIVLEKAKRMVECGHDVVILLDSITRLARAYNTVSPASGKVLSGGVDANALH
                     RPKRFFGAARNIENGGSLTIIATALTETGSKMDEVIFEEFKGTGNMELQLDRRLSNKR
                     IYPAVDILASSTRREDLLLNQLTLNRLWILRRYLSDMNPIEAMEFLKDRLEKTESNEE
                     FLASMND"
     gene            complement(3069..4568)
                     /gene="tilS"
                     /locus_tag="PKY68_04230"
     CDS             complement(3069..4568)
                     /gene="tilS"
                     /locus_tag="PKY68_04230"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOH24277.1"
                     /translation="MDLLNKVQSYIEEHGLLDAKDKVLVGFSGGADSLALLDVLLNLG
                     YNCVAVHMNFQLRGEESFRDEAFVADFCREHSVPLCVERVDTNTYARNNSLSIEMAAR
                     KLRYDYFHRMLPETGAAKIAVGHHIDDSIETFFINLLRGSGIRGLKGISPVQGKVVRP
                     LLCVDRQEILSWLDHKKLSFVTDSSNEGIVYRRNKIRHQVLPLLQSIEPSAAAAILRS
                     MQHLAAPAALYEQAIAEAMKRLNLKPAIRDGQQGFSETPISITVPFPSPDAQRNDFSE
                     TPISINPALAGSVKASTKVTVPAENDLEQYTTLSISALEQEPLAADILYEWLSGYGFD
                     FRNVEQIWTRRKGQSGKQYFSETHRLLLDRGRFLLSEKKALDQGVYELNEGEYVWTEP
                     VKLKMSILEVGEAFPRSSACACLDAGKLQFPLILRRPRPGDSFVPLGMKGRKLLSDYF
                     CDSKKNLFQKESCWLLISGQDIVWVLGERIDHRYRVTNSTKRIYCFSLL"
     gene            4685..5704
                     /gene="pheS"
                     /locus_tag="PKY68_04235"
     CDS             4685..5704
                     /gene="pheS"
                     /locus_tag="PKY68_04235"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOH24278.1"
                     /translation="MIERIETLSNQVDTWSAQDLKELDDLRVKYLSKKGEISQLFNDF
                     RSVPGEQKRLVGQKLNELKDKAQQRLNELKELLDSQKQSNRNYDLSRSAYPHPLGTRH
                     PLSIVRNNIIRIFSMLGFTIAEGPEIEDDWHVFSSLNFPPEHPARDMQDTFFIEKNPD
                     LVLRTHTSSVQTRVMEKTSPPIRIICPGRVYRNEAISYRAHCFFHQVEALYIDKDVSF
                     ADLRQLLEYFCKEMFGEDTKIRLRPSYFPFTEPSAELDISCNICHGKGCPFCKHTGWV
                     EILGCGMVDPNVLENCGIDSSIYSGYALGMGIERIANLKYQIKDLRMFSENDLRFLEQ
                     FRAAH"
     gene            5704..6342
                     /gene="nth"
                     /locus_tag="PKY68_04240"
     CDS             5704..6342
                     /gene="nth"
                     /locus_tag="PKY68_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOH24279.1"
                     /translation="MTLQERYKQVIEWFSANMPEARSELDFRNPYELLVAVILSAQCT
                     DKRVNTITPAFFEAFPDAETLSQSSVEEVFEYIRTCSYPNNKAKHLVGMAQALCREFN
                     GQVPSTMDELLTLPGVGRKTASVVLVCAFNKAAMPVDTHVFRVANRLGLTPGAKTPEQ
                     SEVLLMKHFPEHLLGVAHHWLILHGRYVCQARRPKCLDCGLTSCCEYFNRNK"
BASE COUNT         1528 a         1564 c         1527 g         1826 t
ORIGIN      
        1 ccttgagccc ggcaatctca ccggcttctt tggtagcctg acgctgagag tcgttaaagt
       61 aggccggcac ggtgataacc gcttcggtca cttcgtgtcc caggtaatcc tcggctgtct
      121 tcttcatttt ttgcagtacc atggccgata tttcctgcgg cgtgtagttt ctgtcttcga
      181 tctgtatgcg aggggtgttg ttttcgccct tggtgactgt gtagggcata cgttttacgt
      241 cggtttgaac attatcaaaa cgttctccca taaagcgttt gatggaagaa acggtgtgtt
      301 tcggattggt aatggcctgt cgtttggccg gatcacctac cttgcgttct ccgccatcga
      361 tgaatgctac gatagaagga gtggttcgtt taccttcact gttggctatc actaccggat
      421 cgctgccttc caacacggca acgcaagaat tggtggttcc taagtctatt ccaataatct
      481 ttcccataat tataattctt tattcgtttg aaattttatt ttctaatctg tctgtctgta
      541 aatctttgtt gataatcaag ttgttttttg gtaatcacct atcatttctc aaaagtcgtg
      601 ccatagggtc aacaaaacaa aatctgccga attgtggcag aagcgctgac aattcggcag
      661 attatatgaa tataaaaatg ggaagtgctt agtcgttcat agaggccaga aactcttcgt
      721 tgctctctgt tttttcgaga cggtctttca aaaattccat agcttcgatg gggttcatat
      781 ccgacaaata acggcgcaat atccaaagcc tgttcaaggt gagctgattg agaagtaaat
      841 cttcgcggcg ggtgctggat gccagaatgt caacggccgg ataaatcctt ttgttggaca
      901 aacggcggtc gagctgcagt tccatattgc cggtgccttt gaattcttcg aaaatgactt
      961 catccatttt ggatcccgtt tcggtcaatg ccgtggcaat gatggtaaga ctgcctccat
     1021 tttcgatgtt acgggcggct ccaaaaaagc gtttgggtct gtgtagggcg ttggcatcga
     1081 cacctcccga caaaaccttg cccgaagcgg gcgacaccgt attgtaggct ctggccaggc
     1141 gggtaatgga gtcgagcagt atcaccacgt cgtgtccgca ttctaccata cgtttggctt
     1201 tttcgagcac tatgccggca atcttgacat gtcgttcggc cggttcgtcg aaagtggatg
     1261 aaatcacttc ggctctgacg ctgcgggcca tgtcggtgac ttcctcggga cgttcatcta
     1321 taagaagaat gatcatatac acttccggat ggttggccgc aatggcgttg gcaatatcct
     1381 tcagtaaaac ggttttaccg gttttgggtt gggacacaat caatcctctt tgccccttgc
     1441 ctatgggcga aaacatatct accaccctaa ccgaagtatt ggcataggct tttgtgccgg
     1501 taagattgaa tttttcttcg ggaaacaggg gagttaaatg atcgaaagac aggcggtctc
     1561 taacgtatga aggatcgcgt ccgttgattc tttcgacccg caccagcgga aagtatttct
     1621 ctccttcttt gggcggacgg ataggtccca gaaccatatc gccggttctc agaccgaata
     1681 atttgatttg cgattgtgaa acgtaaatat cgtcgggcga agacatgtaa ttgtagtcgg
     1741 aagaacgcag aaagccgtag ttttccggca taatttccaa acaaccgcta cccataagga
     1801 tgccttcgaa ttcaaaaatc ttttcttctt cggttttggg caaacgttga cttttatagc
     1861 cggcgttgtt gccctgatgt ttttgttgac gaccctggtt gtaggtgtcc tgtgctttag
     1921 gaagcaatcc ggccagtctc cgggcctcga tcaaagcctc ttcgtctgca gtctgcagag
     1981 agggtttctc ttcggtatcg atttgagtgc tttcggtgct taattcttcg tcggtgcttc
     2041 cttcgtacag agctcctgcc tgtgtgtctt cgggagcacg tccttttttg ggaacgggtt
     2101 tgtcttcggc agaaggcttg tcttcggagg aagcttttcc tgttggggtt tttccgcctt
     2161 ctccggccgg cttaccgtct tcttttttgt atccggaagc accgggtttt tgctgctttt
     2221 gagcatcggc agcatctttt gcggcggaac tcttctttgt ttgtgtagcc ttatcctctt
     2281 gccggacttg cgcctcttgc tcttttgctg cttcggcagt cttaggtgat ttgcccttcg
     2341 gacttgtctc ctgagtgctc atagttgcgt ttcccgatgc actctcttcg tctgacttct
     2401 ttttcctggg ccgaccgggc tttctgggtt tttgctcttc actttgtgtt gcaacatcgc
     2461 ttgaaacgat tggctctttt tttgtcactg tctctttttt cgatggagaa tcctttttct
     2521 gtacagagtc ctttttctgc gcagattctt ttttgtctgc acttgtccgg cttgcctttt
     2581 tgctcaatac ttctttttgt ataacatcca gatctgttac gggcaaagaa gaggacagaa
     2641 gattcttatc ctgttgcgtc tttttggacg agtcggatgg attgctttct ttggctttat
     2701 cttgcttttc gtttttggga attctcttgc gcttgcgacg ttcgtcggca gcattttctt
     2761 tcatttgggc cactgtacgg ctggccgact gaagtgcctg ttcatccaga atagcgtata
     2821 tcaaggcttc tttggatgaa ctgtccactt ttttaatgtt aatggattta gcaagctcta
     2881 taagctcttg caaactcttt gcgttgagtt gcacaatatt gtagttgggc ataaaaagaa
     2941 aagaaaaaaa ctatatgtta ttttgatgac gtgaggcaga aaatgccttc gtgaaggaac
     3001 gccgcaaagg taattaattt atttgtttcg acaaaataaa ataccaccgg ccgggctatt
     3061 taagtgcatt ataacagact aaaacaataa atgcgttttg ttgagtttgt tacccgataa
     3121 cgatgatcaa tacgctctcc cagcacccag acaatgtcct ggccggatat gaggagccag
     3181 caggactctt tttggaataa attctttttg gaatcgcaaa aataatcgct gagcaattta
     3241 cgtcctttca tccccaaagg cacaaaacta tctccgggcc tgggtctgcg caaaatcaag
     3301 ggaaattgca gtttaccggc atctaggcag gcacaggcgg acgaacgggg aaaggcttcg
     3361 ccgacttcca aaatcgacat cttcagcttt acaggctccg tccagacata ttcaccctca
     3421 ttcagttcat agacgccttg gtctaaagcc tttttttcgc tcaacaaaaa gcgtccccgg
     3481 tcaagcaaca ggcgatgagt ttccgaaaaa tactgcttgc cgctctgtcc ctttcgcctc
     3541 gtccagattt gttccacgtt gcggaaatca aagccataac cggaaagcca ttcgtaaaga
     3601 atatcggcgg ctaagggctc ttgttccaga gctgagatgc ttagagtcgt gtattgctca
     3661 aggtcgtttt ctgccggcac agtcactttt gttgaggctt taacactacc tgctaaagcg
     3721 gggtttatcg atatgggggt ttctgaaaaa tcatttctct gtgcatcagg agatgggaag
     3781 ggcaccgtta tcgatatggg ggtttctgag aaaccctgtt gtccgtcccg aatagccggt
     3841 ttcaggttga gtcttttcat cgcctccgct atggcttgtt cgtatagagc tgccggagcg
     3901 gccaggtgtt gcatgcttcg aagtatggcc gcggcggccg aaggttctat gctttggagc
     3961 aaaggaagca cctgatggcg tattttgttg cgccggtaaa ctattccctc gttgctgctg
     4021 tcggtaacaa acgagagttt tttatgatcc agccacgaca aaatctcctg cctgtctacg
     4081 caaagcaagg gtctgacaac tttgccctga acgggactga tgccctttag cccgcgaatt
     4141 ccgctgccac gcaaaagatt gataaaaaaa gtctctatgc tatcgtcgat gtgatgccct
     4201 acggctattt tagcagctcc cgtttcgggc aacatccggt ggaaataatc gtagcgcagt
     4261 ttccgggcgg ccatttcgat ggaaagagag ttgtttcttg cgtaagtatt ggtatcgaca
     4321 cgttccacac acaaaggaac ggaatgctcg cggcagaaat ccgcgacaaa ggcttcgtca
     4381 cgaaaggatt cctctcctct gagttgaaaa ttcatatgta cagccacaca gttgtagccc
     4441 aggttaagca gcacatccag caaggcaaga gaatctgctc ccccgctgaa tcccaccaga
     4501 accttgtctt tggcatccaa taaaccgtgt tcttcaatat acgattgaac cttgttcagc
     4561 aagtccataa taagttgcct ttttccgtag aggcaaaggt agaaaaaaca tccgatatgc
     4621 tctttgccct acccaagtat gaaaataaac tcgtacattt gcgggtcaaa aattttgcat
     4681 atctatgatt gaacgtatcg aaacactctc gaaccaagtc gatacctggt cggctcagga
     4741 cctgaaagaa ctcgatgatt tgcgggtcaa gtacctcagc aaaaaaggcg aaatcagtca
     4801 attgttcaac gacttcagat cggtgcccgg cgaacagaaa agattggtcg ggcaaaagct
     4861 gaacgagctc aaagacaaag cacaacaacg cttgaatgag ctgaaagagc tgctcgacag
     4921 tcaaaaacaa agcaaccgga actacgattt aagccgcagc gcctatcccc accctctggg
     4981 gacacgtcat cctttgtcaa ttgtaaggaa caatataata cgtattttct cgatgctggg
     5041 ctttaccata gccgaaggcc ccgaaatcga agacgactgg catgtgttca gctcgctgaa
     5101 tttcccgccc gaacaccctg ccagagatat gcaggatact ttttttatag aaaaaaatcc
     5161 cgatctggtt ttgcgcactc atacttcgtc ggtacaaacc agggtgatgg aaaaaaccag
     5221 ccctcccatt cgcatcatct gcccgggcag ggtgtaccgc aacgaagcta tttcctacag
     5281 ggcgcattgt ttctttcatc aagtcgaagc gctctacata gataaagacg tctcttttgc
     5341 cgatctgagg caattgctcg aatacttttg caaggaaatg ttcggagaag ataccaaaat
     5401 ccgcctccgc ccctcctact tcccctttac ggaaccgtcg gccgaactgg atatttcctg
     5461 caacatctgc cacggcaaag gttgtccctt ttgcaagcac accggttggg tggaaatttt
     5521 gggctgcggc atggtcgatc ccaacgttct cgaaaactgc ggaatcgaca gctccatcta
     5581 cagcggctat gccttgggta tgggcattga acgcatcgcc aacctcaaat accagatcaa
     5641 agatttacgt atgttctccg aaaacgacct ccgttttctg gagcaattcc gggctgccca
     5701 ctgatgactc ttcaggaacg ttataagcag gtaatagagt ggttttcggc aaacatgccc
     5761 gaggcccgca gcgaattgga cttccgaaat ccttacgaat tactggtggc tgtcattctg
     5821 tcggctcagt gtaccgataa aagagtgaat acaatcactc cggccttttt tgaagccttc
     5881 cctgacgcgg aaacactgtc tcaaagcagt gtcgaagagg tttttgaata cattcggacc
     5941 tgctcctatc ccaacaacaa ggccaaacat ctggtaggca tggctcaagc tttatgccgg
     6001 gaattcaacg gacaagtgcc ttcgactatg gacgaattgc tgaccttgcc gggcgtaggc
     6061 cgcaaaacag ccagcgtggt attggtttgt gctttcaaca aggcagctat gcctgtagac
     6121 acccatgtat ttagggttgc caaccgtctg ggactcacac ccggtgccaa aactccagaa
     6181 caaagcgaag tactcctgat gaaacacttt cccgaacact tgcttggggt agctcatcat
     6241 tggctgatcc tgcacggacg ttacgtctgc caggcccgca ggcccaaatg cctcgattgc
     6301 ggccttactt cctgttgtga gtatttcaac cgaaataaat aatacggcca ttccgaccgg
     6361 ctaatcacgg taaaagacga aacggcaggc catacagctg cttgccggcg gtctccggcc
     6421 aacaggtatg ccggcggtat gacct
//
LOCUS       DAOSGL010000095         6439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_10528_length_6439_cov_3.834743, whole genome shotgun sequence.
ACCESSION   DAOSGL010000095 DAOSGL010000000
VERSION     DAOSGL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10528_length_6439_cov_3.834743"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /locus_tag="PKY68_04245"
     CDS             complement(<1..2021)
                     /locus_tag="PKY68_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24280.1"
                     /translation="MKKLYLFMGGLLLAASMNLSQAQNPMTEVKIGDLIYISHTGTGR
                     VWSGINESSNIQIRDPKIAFTDTAQIWEIVPAQVAGTDSTLNDADFYYLQEKTGLQRF
                     AASDGSWNSRVNSAVGVIADTTRYYWTFRRSATLENVFHVANGIKFTIDSATWTVKSV
                     SRYMGTDGTGNGSSLYTDKASSNAGAPYYIYDAKTYAKFVVAKAISDAENLIQLLNNN
                     NLSAYGDTLQTALDAVKLIDTTKTYDQYTQADQAKLDELKVVSAIQAIADIKTWQNAV
                     IAFQNKITEAEGKVVLDPKYPASIADSLNMAITDAKAYGDTVTYQGSTVAEIQAEQAV
                     LQGFINRMTDRLKTVDKLIAKIDEAKAVDQGAAPYPEQSKADFNAAIATAESYLAGIT
                     GTTALADIEQATAILNLQMYTLLISVATEENPIEVTDLISDPSFEESSGYTLSAKWKD
                     ASVVAGGDRRVNSSIVVTDGNQCWNSWSNNFTSMNVYQDLTNLPNGVYKVTCDAITQE
                     GYITDQHLYATTFETTQSTVLTATGFNTTPEAWETLSTQFVVFDGTARIGFASTSGGA
                     QAGWFCIDNFRLYYLGVDPDAVETMFRVLLTQIQSDRMDYVVPLGEDPLLDAALEAAN
                     TFLEGEYTSEELDVMVARTSAAWNRFKSSQTAALSLNDYIVEATEMYETA"
     gene            2486..5209
                     /locus_tag="PKY68_04250"
     CDS             2486..5209
                     /locus_tag="PKY68_04250"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOH24281.1"
                     /translation="MKKIRILSLLLLLASLFQLQAARQRISRDTQLAFVSRDTIEVAE
                     GVELRLTDANPIRNNALIILEGPEAWVYLLEVRPTKVISSYLKSIKLGSELTSLVHRS
                     NCRAEIYRHGCVVIPQNSFYQPLEAFTEPNFGGSSATYGILTYHTDLGAMTGAIRSFK
                     LKKGYMATLATAADGTGFSRVFIADSEDLEISQLQVELDRNVAYIRIFQWRWPSQKGW
                     CWGPSAARLDSVQATWYYDWDANGMDNYMDAEYVPMRHNAGWQAFSVIESRRYSTHVL
                     GFNEPDNPNDDGPMTVDETVAMWPKLMKSGLRLGSLATTDGGLNYLYEFMDKCDALNY
                     RVDFVAVHFYRGGQSPWNLYSFVKGVHDRTGRPIWITEWNNGANWTSETWPSNVSEQQ
                     AKQLRDMTAFFNMLDTCSFVERHAVYNAVEYKRYMLDPDNGNRLTPAGKMMRDFKAKM
                     AYNPAKEFEPLPWKIKAPNLISGMIASDFKSYNLRLGSNPNGTLAAAYLVEKSVGSED
                     FEQIDSVTDLSLTVLNYPVDYTRSGSTSFRMRTVGKNGEYSGYTKAVSYHTTPADSVQ
                     LGELAIANTEWNPLFFGQEHAAEPTMILGAASFRNLVAFAPRARNLTKKKVDFKLFPY
                     EYVSNQTISNAESLPFLFVSKGVHDWGGLKAIAGKSAVGGNWTNISFPEAFETVPVVF
                     AQQITSVNSTATAIRIRNVTKEGFQAKIAKELAQTYNTVAEAINWLAVSPGEGKLNGR
                     DIVVGSTASTALGTSSSSWQVATIKWGKRLTNPLFFAFMQTANDERAAVVRRHTLSST
                     TATIFKQTEGSQSVAVQKEGAGWLVIDNPEPSGLNMTEREESGLRLAIHNGILFFETQ
                     SPGNQQVEILDAWGRVVLQTSAGLGNRLDVSTLPRGCYLLRVGNTFSKFIR"
     gene            5230..>6439
                     /locus_tag="PKY68_04255"
     CDS             5230..>6439
                     /locus_tag="PKY68_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24282.1"
                     /translation="MKKSLILIVALLLQSLGLYAADPVVTTNPEVVRGATLAFAFGSV
                     DPRGNTVTEKGICWATSPYPGIEDNTVTATALSYRCRLDNLPPASRLYIRAYAKTSDN
                     RVFYGENETFTTIPKGKVTYTVNQPAAEDIDHYNRIKASAEISVDYYNNLTSISNKHL
                     WFNYSSGVPTADANYDGWIRFGSNASYQQVGTALHEMAHCIGVGTHGNWTGLLLKNGV
                     YQGRRANRVLQLMTGDPKTFVRGDNTHFWPYGINGAHEDDRNEMTYIINTMLVQAMGE
                     DGLPPSGGFATPAETFAAKAGKKYYIKSEAVEQGRDSMFLVAGTNNSLRLETMTSAQA
                     LADDRAAWYFHFDPATQYYFIRNAATGKHIGLNAAQAYNGFTLMDSTQAVVEKLRIQL
                     IRNRNDIMSGD"
BASE COUNT         1626 a         1534 c         1610 g         1669 t
ORIGIN      
        1 gctgtttcgt acatttcggt agcttcaact atataatcgt tcaggctcaa agctgctgtt
       61 tgagagcttt tgaagcgatt ccaggccgca ctggtccggg caaccataac gtcaagttct
      121 tccgaggtgt attcgccttc gaggaaggta ttggcggctt cgagggctgc atccagtaag
      181 ggatcttcac ccaggggaac cacgtaatcc atgcggtcgc tttgaatttg agtgagcagt
      241 actctgaaca tggtttcgac ggcatccgga tcaacgccta aataatacag gcggaaattg
      301 tctatgcaga accagccggc ctgagctcct cccgaagtgg aagcaaaacc gatacgggcg
      361 gtgccgtcaa agaccacaaa ttgggtgctg agtgtttccc aggcttcggg agtggtattg
      421 aacccagtgg ccgttaacac agtcgactgg gtagtctcaa aagtagtggc atacagatgt
      481 tggtcggtaa tgtagccttc ttgtgtaatg gcatcgcagg ttaccttgta aactccgtta
      541 ggcagattgg tcaggtcctg gtaaacattc atcgaggtga agttgttcga ccagctgttc
      601 cagcactgat tgccgtcggt taccacaatg gagctgttta ccctgcggtc gccgccggcc
      661 accacactgg catctttcca tttggcactt agggtatagc cactggattc ttcgaaggag
      721 gggtcagaaa ttaaatctgt tacttcgatg gggttttctt cggtggccac cgaaatcagc
      781 aaagtgtaca tctgcaggtt cagtattgca gtagcttgct caatatcggc cagggcggtg
      841 gtgcctgtaa taccggccag atagctttcc gctgttgcaa tggcggcgtt gaaatcggct
      901 ttgctttgtt cgggatacgg agcggcacct tgatctacag ccttggcttc gtcaattttg
      961 gcgatcagtt tatccaccgt tttcaatctg tcggtcatac ggtttataaa tccttgcaaa
     1021 acagcttgtt ctgcctgaat ttcagcgact gttgatccct ggtaggtaac agtgtcgcca
     1081 taggccttag catcagtaat ggccatattc agagagtcgg caatgctggc aggatacttg
     1141 ggatcgagca ctacctttcc ttcggcttcg gtgattttgt tttggaaagc tatcactgca
     1201 ttttgccagg ttttgatgtc ggcaatggcc tgaatggcgc taaccacctt caattcatcc
     1261 agcttggcct gatcggcctg ggtgtactga tcgtaagttt tggtggtatc gatgagtttg
     1321 acggcatcca aagctgtttg aagcgtatct ccataggccg aaagattgtt gttgttgagc
     1381 aactgaatca aattttctgc gtcgcttata gctttggcaa caacgaactt ggcataagtt
     1441 ttggcgtcgt agatataata aggagctccg gcattgctgg aggccttatc ggtataaagg
     1501 ctgcttccat tgccggttcc atctgtaccc atatatctgc tcaccgattt tacggtccag
     1561 gtagcagaat ctattgtaaa tttgatacca ttagccacgt ggaatacatt ttcgagtgta
     1621 gcggaccgtc taaaagtcca gtaatacctc gtcgtatcgg ctataacgcc tacagcggaa
     1681 ttaaccctgg aattccagct tccgtcggag gcagcaaagc gctgcaggcc ggttttttct
     1741 tgcagatagt aaaaatcggc atcgtttagt gtagagtcgg tacctgccac ttgtgcagga
     1801 acaatttccc agatttgagc ggtatccgta aaggctattt tcggatcgcg tatctgaatg
     1861 ttggaacttt cgttgattcc gctccagacc ctgcctgtac cggtgtgtga aatgtaaatc
     1921 agatcaccga ttttaacttc ggtcatgggg ttttgagcct ggctcagatt catacttgcg
     1981 gccaatagca ggccacccat aaataagtag agttttttca tatgcgtacg tgtttaaaaa
     2041 ggatgttggt tagaattgat ttgaaatgtt aggttagact agattttcgt ttccgcccag
     2101 gaatgtacaa aagaacgaga gaaaaaccaa gttcgggtct aaaaaacatt cagatatggc
     2161 ctcataatcg ttcaggtctg tttagacctt tgtaggcccg tatgaggctt gcggtaaaaa
     2221 ttttcacttt aagttaaaaa gctcagaatc aatttcaaag tgaatgaaaa gagttgattt
     2281 tgcctttagt ttgtactcat ttgaacgatt attaaacctt cttgcgcaag gacatgatta
     2341 gttttgtcgt agtagtctaa atcaatcaat caaccaatca atcaatcaat caaccaatca
     2401 atcaatcaat caatcaatca atcaatcaat caatcaatca atcaatagat ttaggtatat
     2461 ttgttctttg tttcaaaacg gagttatgaa aaagatacgc attctgagtt tacttttgtt
     2521 gctggcaagc ctttttcagt tgcaggcagc caggcaacgt ataagcagag atacgcaact
     2581 ggcctttgtg tctcgcgaca ccatagaagt ggccgagggg gttgaactac gtctgacaga
     2641 cgccaatccc atacgaaaca atgctctgat tattctcgaa ggaccggaag cctgggtcta
     2701 tttgctcgaa gtgcgaccga ccaaagtcat ttcttcttat ttaaagagca ttaaactggg
     2761 cagcgaactg acctctctgg tacatcgttc gaattgccgt gcggagattt accggcacgg
     2821 ttgtgtagtg attccgcaaa acagttttta ccagcctctg gaagccttta ccgaacccaa
     2881 tttcggaggc agttcggcaa cttacggtat tcttacttac catacggact tgggagctat
     2941 gacaggagcc atccgctcat ttaaacttaa aaaaggctat atggctacgt tggctaccgc
     3001 agccgacggg accggtttta gccgggtctt tattgccgac agcgaagacc tcgaaatttc
     3061 ccagttgcag gttgagctcg atcggaacgt ggcctatatc agaattttcc aatggagatg
     3121 gccttcgcaa aaaggctggt gctggggtcc cagtgctgcc cgtctcgatt cggtgcaagc
     3181 cacctggtat tacgactggg atgccaacgg aatggataat tatatggacg ccgaatacgt
     3241 tcccatgaga cataatgccg gttggcaggc tttcagtgtg atagagtcgc gaagatattc
     3301 tactcacgtt ttgggcttca acgaacccga caaccccaac gacgatggtc ccatgacggt
     3361 ggatgagacg gtggccatgt ggcccaaact gatgaaatcg ggcctgagac tgggttccct
     3421 ggcgactacc gacggagggt tgaattatct gtacgaattt atggataaat gcgatgcgct
     3481 taattacagg gtcgattttg ttgcggtgca tttttacagg ggtggccaat cgccctggaa
     3541 tttgtactct tttgttaagg gagtgcacga ccgtaccggc cggcctattt ggatcaccga
     3601 atggaacaac ggagccaact ggaccagcga aacctggcct tcgaacgtgt cggaacagca
     3661 ggctaaacaa ctgagggata tgaccgcctt cttcaatatg ttggatacct gttcctttgt
     3721 agaacgtcac gctgtttaca acgcggtgga atacaaacgc tacatgttgg atcccgataa
     3781 cggcaaccgc ctgactcccg ccgggaaaat gatgcgcgat ttcaaagcca aaatggccta
     3841 caatcccgcc aaggagtttg aacctctgcc ctggaaaatc aaggcaccca atctgatttc
     3901 gggtatgata gcctccgatt ttaaaagcta taacctgcgt ttgggaagca atcccaatgg
     3961 cactttggca gccgcctacc tggtggaaaa gagtgtaggt tccgaagatt ttgaacagat
     4021 tgattcggtg accgatctca gcctgacggt gttgaattat ccggtcgatt atactcgttc
     4081 cggttctact tcgttcagga tgcgaacagt aggcaaaaat ggcgagtact ccggttatac
     4141 caaagcagta tcgtatcata cgacccccgc cgactccgtg caactgggtg agctggccat
     4201 agcaaatacc gaatggaacc ctttgttttt cgggcaggaa cacgctgccg aaccgactat
     4261 gattttaggt gccgcatcgt tccgtaatct ggtggcattt gcgcccagag cccgcaatct
     4321 gaccaagaaa aaggttgatt ttaagttatt tccctacgaa tacgtcagca accaaaccat
     4381 cagcaacgcc gaatcgcttc ctttcctgtt cgtctccaag ggtgtacacg attggggagg
     4441 tctcaaggcc atagccggca agagcgcagt cggcggcaac tggaccaata tcagttttcc
     4501 cgaagctttc gaaacggtgc ccgtggtctt tgcccaacaa attacttctg taaactcaac
     4561 agccactgcc attcgtatcc gcaatgtaac caaagaagga tttcaggcta aaatcgctaa
     4621 agaattggct caaacctata ataccgtagc cgaggccatt aactggctgg ctgtttcgcc
     4681 cggcgaaggc aaactcaacg gccgcgatat agtggtgggt tctaccgcct ccacggcttt
     4741 gggtacgtcg agcagttcct ggcaggtagc taccataaag tggggtaaaa gattgaccaa
     4801 tcccttgttt tttgccttta tgcaaacggc caacgatgag cgtgccgccg ttgttcgcag
     4861 acatacgctc agttctacca cagccacgat ttttaaacaa accgaaggca gccagagtgt
     4921 tgccgtacaa aaagagggag ccggctggct ggttatcgac aatcccgagc cttcaggtct
     4981 gaatatgacg gaaagggaag agtccggact gcgtttggcc atacacaacg gtatattgtt
     5041 tttcgaaacg caaagtcccg gcaaccaaca agtggagata ttggacgcct ggggcagggt
     5101 agtcttgcaa acctctgccg gtctggggaa tcggcttgat gtttccacat tgccccgtgg
     5161 ctgttacctg ttgcgggtgg gcaatacatt cagtaagttt atccgatgat ttttaagaag
     5221 ttgataatta tgaaaaagag tttaatatta atagtggcct tgcttttgca gtctttaggg
     5281 ctgtacgcag ccgatcccgt ggtaacgacc aaccccgagg tggtgcgcgg agccacgctg
     5341 gcctttgcct ttggatcggt cgatccccgg ggaaacactg tgacggaaaa aggcatttgc
     5401 tgggcgactt cgccctatcc cggcatagaa gacaatacgg ttacagccac tgcgttgtcg
     5461 taccgttgcc ggctggataa tttgcctcct gccagcaggc tttacatcag ggcctatgcc
     5521 aaaacctcag acaatcgggt tttttacggt gaaaacgaaa cattcaccac catccccaag
     5581 ggcaaagtta cttacacggt caatcagccc gctgccgaag atatcgatca ttacaatcgc
     5641 attaaagcct ctgctgaaat atcggtggat tactacaaca acctgacttc gatctccaac
     5701 aaacacctct ggtttaacta cagttcgggg gtgccgactg ccgatgccaa ctacgacgga
     5761 tggattcgtt tcggttcaaa cgccagttat cagcaggtgg gtaccgcttt gcacgaaatg
     5821 gcgcattgca taggcgtggg tacccacggc aactggacag ggctgctttt gaaaaacggg
     5881 gtgtatcagg gtagaagagc taaccgggtt ttgcagttga tgaccggaga tcccaagact
     5941 tttgtcagag gcgacaacac gcatttctgg ccttacggga taaacggagc ccacgaggac
     6001 gaccgtaacg agatgaccta catcatcaac accatgctgg tacaggccat gggcgaagac
     6061 ggcttgcctc cttcgggtgg ttttgccact cccgccgaaa cctttgccgc caaagccggc
     6121 aaaaaatact acatcaaaag cgaagctgtg gaacagggac gcgattcaat gtttctggtg
     6181 gccggcacaa acaattcgct aaggctcgaa acgatgactt ctgcccaggc cctggcagac
     6241 gaccgggctg cctggtattt ccacttcgat ccggctacac agtattattt tatccgcaat
     6301 gccgcaacag gcaaacatat tggtctgaat gccgctcagg cttacaacgg ctttacactc
     6361 atggacagca ctcaggctgt tgtagaaaaa cttcgcattc agcttatccg caatcgcaac
     6421 gatattatga gcggggatg
//
LOCUS       DAOSGL010000096         6404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_10616_length_6404_cov_3.484013, whole genome shotgun sequence.
ACCESSION   DAOSGL010000096 DAOSGL010000000
VERSION     DAOSGL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10616_length_6404_cov_3.484013"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKY68_04260"
     CDS             <1..336
                     /locus_tag="PKY68_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH24283.1"
                     /translation="GVLSGGERNRLHLALNLKSEPDVLLLDEPTNDIDVNTLRALEEG
                     LENFAGCAVVISHDRWFLDRICTHILAFEGNSRVFYFEGSYTEYEENKRMRLGDEGPK
                     RIRYRQWNE"
     gene            361..1578
                     /locus_tag="PKY68_04265"
     CDS             361..1578
                     /locus_tag="PKY68_04265"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOH24284.1"
                     /translation="MQLRINKLLTALLLGTSFLLQSCRQNGQVGQLRTETDSTLWSVR
                     MAESEMKRFPELWQFDHGRRLFFGYTQGLGAKAMLAMWQSTGDEKYYRYVYNWANSMV
                     GSKGQIHLYAPKDYNLDFVNSGKILFDLYAHTGEPRFKMAIDSIMDLQLRQQPRTHQG
                     GFWHKKIYPYQMWLDGLYMAGPFIARYARELDEPEWTDEVIHQLILVGRQTYDSKTGL
                     FYHAWNSNREQAWANPQTGCSPNFWGRSIGWYAMALVDVLDYIPEEHPRRGEVIKLLK
                     KLSAGMLRWQDGRTGLWYQVVDQGQREGNYLESSVSAMMMYTYAKGYNKGYLGRKYLK
                     AANEVLDGLIAHRIVANEDATISLTYCCAVSGLGGNPYRDGSFEYYVNERIRNNDGKA
                     TGPFILGCLELGR"
     gene            complement(1671..2570)
                     /locus_tag="PKY68_04270"
     CDS             complement(1671..2570)
                     /locus_tag="PKY68_04270"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOH24285.1"
                     /translation="MQKIKAAIVGYGNIGKYLLEALQSSPDFELTGIVRRDPQGVQPV
                     ELRPYRVVASVKELPQTEVAILCTPSRSTQKYAAEMLNLGIHTVDSFDVHSDIWDVKQ
                     KLDKIAKENKRVSVISAGWDPGSDSVVRALMEASVPKGITYTNFGPGMSMGHTVAVKA
                     VPGVKAALSMTIPLGSGVHRRMVYIELEKGYDFNKVASAIKADAYFVHDETHVVQVED
                     VNALLDMGHGVHLVRKGVSGKTQNQQLEFSMRINNPAFTAQLLVAAARASMKQAPGCY
                     TMIELPMIDLLPGNREDLIRRLV"
     gene            complement(2610..3695)
                     /locus_tag="PKY68_04275"
     CDS             complement(2610..3695)
                     /locus_tag="PKY68_04275"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOH24286.1"
                     /translation="MISTALLIFFTAFIVSCIAMPNIIKISVKKLLFDAPTEERKIHK
                     KNISNLGGIGIFIGFFFSILLYRIICDSLELSSLAAAAVLLFFVALKDDLIPSSPISR
                     LFYQVLIASIIVFIGQVHFDHIPIFEAGTTMRRLVNILFSLFFVVGIINAMNFIDGID
                     GLAASLSVFLSLIFGYIFYQYGQLNYSYTSLALGGAVLGFLIFNFAPARIFMGDIGSM
                     LIGVFMAIFGIQLANMDTLPLGLLQIRYPGTIMFALLIVPIMDLITVVGIRLYLKKSP
                     FEADKRHLHHRLLVLGLSHPAICFSLLLFNILVLAVAFLLQKTDSSLWAALAITLLAA
                     TFELLVLWLYSKKKKLDDKELDIVNHK"
     gene            complement(3822..>6404)
                     /locus_tag="PKY68_04280"
     CDS             complement(3822..>6404)
                     /locus_tag="PKY68_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24287.1"
                     /translation="HFRAYGASQMTLPPDKGVIMDTALNLARSAKVAGSEMYSSGYIA
                     RYPNNPGTMNHYDMNLCYIDELLWHFRWTGDLEFVREMWPVLTRHLAWEKRCFDPDDD
                     GLYDAYCCIWASDALQYNGGAVTHSTAYNYRANLMAAELAELLGLDARPFHNEADKIR
                     EAVGRVLWLSQKGYWAEFRDAFGLQRLHEQAAVWTIYHAIDAELHDPFTGWQALNYIE
                     HEIPHIPLKINTKGLSKNDMPEADLAVISTSNWQPYVWSVNNVAFSEIMHTALACFQG
                     GRPEMGFKLMKSAIMDGMYLGGSPGNVGQVSYYDAARGEVYRDFADVVGVYSRAMVQG
                     LFGIEPDLLHNKVYLRPGFPAQWDKASIEIPDLSYRFERKGKTSRWQIHSGFGRDLDY
                     VLELPLRYDQIPLVRINGTELADVNIISSVEKPLLQINCPSGRQFDIEIIEQGNNREV
                     WNFPSVLAIGEELSLPLPGKAEMLDIYDPQAVLQQVQIKDNQFQAVVSGLEGPRSFFV
                     LLKQGDVSWWQVLAPALKPGLEIIGEEGNLDEKQLSFRIRNNSLSRNLQAELFLNGEK
                     IKTLRMPAQSLSDPIRNSKAVRMGSNLVEIRQKGTTLAQARVINWNLPPASPEQQETI
                     DLNSYHNDKLNDIFRFGKYLSPRSPFTTLQIPTQGIGDWCSPMHIVNIDDKGVRKAAG
                     ANNLINTRLGLNFASPGDSLRNNVLFVSHWDNYPTLARLPLQGKAQHAYLLMAGSTNH
                     MQYGLPNGKLCFYYSDGSCDSLLLINPENWPAIEQDAFDDGLAFRLKAPRPWRLLLAT
                     GELSKEPGKLLKLRGAAGRYIPGGAATILDLTLNPGKELSHLEFEALSNEVVLGLMSL
                     TLIR"
BASE COUNT         1633 a         1499 c         1643 g         1629 t
ORIGIN      
        1 ggtgtgctgt cgggcggtga acgcaaccgg ttgcatctgg ccttgaatct caagtcggaa
       61 cccgatgtgt tgttgctcga cgaacccacc aacgacatcg atgtcaacac cctgagagcc
      121 ctggaagagg gcctggaaaa ttttgccggc tgtgcggtgg ttatcagcca cgatcgctgg
      181 ttcttggacc gtatttgcac ccacatcctc gcttttgagg gaaattcccg ggttttctac
      241 ttcgaaggtt cctataccga gtatgaagaa aacaagcgga tgcgtctggg cgacgaaggc
      301 ccaaaacgga tacgttaccg gcaatggaat gaataaccat atctaaaaaa cgaataaagc
      361 atgcaactta gaatcaacaa actactaacg gctctcttgc ttggaacatc ttttcttctg
      421 cagagctgcc ggcagaatgg gcaggtcgga cagctcagaa ctgaaaccga ctctacactt
      481 tggtcggttc ggatggctga atcggaaatg aaacgttttc ccgaattatg gcaattcgat
      541 cacggacgtc gcttgttttt tggatatact cagggtctgg gcgcaaaagc aatgctggcc
      601 atgtggcagt caaccggaga cgaaaagtac tatcgctacg tgtataactg ggccaacagt
      661 atggttggat ctaaggggca aattcacctt tacgccccaa aggattacaa cctggatttt
      721 gtcaattccg gcaagatact ttttgacctt tatgcccata ccggggaacc tcgcttcaaa
      781 atggccatag acagcatcat ggatctgcag ttgcgccaac agccccgtac tcaccaaggc
      841 ggcttctggc ataaaaaaat ctacccctat cagatgtggc tcgacgggct ttacatggcc
      901 ggccctttta ttgcccgtta cgccagggag ttagatgagc ccgaatggac agacgaggtg
      961 atccatcaat tgattttggt gggcagacaa acctatgact ccaaaaccgg cttattttat
     1021 catgcctgga attccaaccg cgaacaagcc tgggcaaatc cgcagacagg ttgttcgccc
     1081 aatttctggg gacgcagcat cggttggtat gccatggcct tggtggatgt gctggattat
     1141 attcccgaag aacatccccg cagaggcgaa gtgataaaat tgctcaaaaa actctccgcc
     1201 ggcatgctgc gctggcagga cggacgcacg ggactttggt atcaggtggt ggatcaggga
     1261 cagcgcgaag gcaattacct ggaatcttcg gtttccgcca tgatgatgta cacttacgcc
     1321 aaaggttata ataagggcta tctgggcaga aaatacctta aggccgccaa cgaggtgttg
     1381 gacggactga ttgctcaccg catagtggcc aacgaggacg ctaccatttc gcttacttat
     1441 tgttgcgctg tttcgggctt agggggtaat ccctaccgtg acggcagttt tgagtattac
     1501 gtcaacgaaa gaatacggaa caacgacggc aaagctaccg gaccctttat tttgggctgt
     1561 ttggaactgg gacgttaaag cttacgactt tccttcctgc cggtttttcc ttcctgccgg
     1621 ttatttggct gtctgagttt tgtttttgta ggggcggctg taaatttgct tcagactaac
     1681 ctgcgaatca agtcttctct gttgccgggc agtaaatcga tcattggcaa ttctatcatg
     1741 gtataacaac ccggagcttg tttcatactt gctctggccg cagctaccaa gagttgggcg
     1801 gtgaaggccg gattgttgat acgcatgctg aattccaact gttgattttg ggttttgccc
     1861 gaaacgcctt tgcggaccaa atgaactccg tgacccatat cgagcagggc attaacgtct
     1921 tcgacttgca caacgtgggt ttcatcgtgt acaaaatagg cgtcggcttt gatggccgaa
     1981 gctactttgt taaaatcata gcctttttcc agttcgatgt agaccatgcg ccggtgtacg
     2041 ccggagccta aaggtatggt catcgaaaga gctgctttga ccccgggcac ggctttgacg
     2101 gcgacggtat ggcccatact catgccgggg ccgaagttgg tgtaggtgat gcctttggga
     2161 acagaagctt ccatcagagc acgaaccaca gagtcgctgc cgggatccca gccggccgag
     2221 atcaccgaaa cccgtttgtt ttctttggca attttatcga gtttttgttt tacatcccaa
     2281 atgtcgctgt gaacatcaaa actgtcgacg gtatggatgc ccaggttgag catttctgca
     2341 gcgtattttt gagtgctgcg gctaggagta cacaaaatag ccacctcggt ttgggggagt
     2401 tctttgacag aggccactac ccggtaggga cgtaattcaa caggctgaac tccttgaggg
     2461 tcacggcgaa caatgccggt taattcaaag tcgggagagc tttgcagagc ttccagcagg
     2521 tatttgccaa tatttccgta tccgacaatg gcggctttaa ttttttgcat agaatgaggg
     2581 attttgaagg gagcaaattt gctttcctct tatttgtgat tgacgatatc gagctcttta
     2641 tcgtcgagtt tcttcttctt ggaatataac cacaaaacca gcaactcgaa ggttgcggcc
     2701 agtaacgtga tagccagggc tgcccaaaga gaactatcgg ttttttgcag caaaaaagcc
     2761 acagccagca caagaatatt gaataaaagt aggctaaagc aaatagccgg gtgacttaga
     2821 ccgagcacta ataaccggtg gtgcagatgg cgtttatctg cctcgaaagg agattttttc
     2881 aggtaaagtc ttatacccac taccgtgatc aggtccatga tgggcacgat gagtagggca
     2941 aacataatgg tccccggata cctgatttgc aaaagaccca aggggagggt atccatattg
     3001 gccagttgaa tcccgaaaat ggccataaat accccaatca gcatggagcc gatatcgccc
     3061 ataaaaatgc gggccggggc aaaattaaag atcaggaaac ctagaacggc tccacccaga
     3121 gccagcgaag tataggaata attcaattga ccgtactgat aaaagatata accgaaaatc
     3181 aggctcagga acacgctcag gctggcagcg agcccgtcta taccgtcaat aaagttcata
     3241 gcgttgatga tgcccaccac aaagaacagt gagaacaaaa tattgactaa acggcgcatg
     3301 gttgtgccgg cttcaaaaat gggaatgtgg tcgaagtgta cctgaccgat aaaaactatg
     3361 atcgaagcaa tcagcacttg ataaaacagc cggctgatgg gagaggaagg gataaggtcg
     3421 tctttgaggg ccacaaaaaa cagcaacacg gccgctgctg ctaaactgct gagctccaat
     3481 gagtcgcata tgattctata gagtaaaata ctgaaaaaga atccgatgaa aatacctata
     3541 ccgcccaagt tggagatatt ctttttatga attttacgtt cttcggtggg ggcatcgaac
     3601 aggagctttt tgaccgaaat cttgatgatg ttcggcatgg cgatgcagga aacgataaaa
     3661 gccgtaaaaa aaatgagtag agctgtcgat atcatacgca aatcatagtt tatactaata
     3721 actcaaaaag tcctgcttta ttgtcgttga aagagaattt tttttagctt ttttttcgta
     3781 taatgttata atatttatac agagcataat agctgttata attatcttat cagggtcagg
     3841 gacataagac ccaaaaccac ttcattactc aaagcttcga attcaagatg actcaattct
     3901 ttgccgggat taagtgttag atccagtatg gtggcagcgc ctccgggaat ataccggccg
     3961 gccgctcccc ttagtttcaa aagcttgccg ggttctttac taagctctcc cgttgccagc
     4021 agcagacgcc agggacgggg agctttgagc ctgaaggcca gtccgtcatc gaaggcatcc
     4081 tgttcgatgg ccggccagtt ttcgggattg atcaacaaca aactgtcgca gcttccgtcg
     4141 ctataataaa aacagagttt accgttgggc aggccgtatt gcatatggtt ggtggaaccg
     4201 gccataagca gataagcatg ctgcgcttta ccctgtaatg gcaaacgggc cagggtgggg
     4261 taattgtccc aatgcgaaac aaaaagaacg ttgttccgga ggctgtcgcc gggactggca
     4321 aaattcagtc ccaacctggt gttgatcaga ttatttgctc cggctgcttt tcgtacaccc
     4381 ttgtcgtcga tattcactat atgcatggga ctgcaccaat cgcctatacc ttgggtgggg
     4441 atttgaagtg tggtaaaagg agagcgcgga ctgaggtatt tgccgaaacg gaaaatatcg
     4501 ttgagtttgt cgttgtgata agagttcaag tcgatggttt cctgttgttc gggagaagcc
     4561 ggaggcagat tccagttgat aacccgtgcc tgcgcaagtg ttgttccttt ttgtctgatt
     4621 tctaccaggt tactgcccat gcggacggct ttactgttgc gaatgggatc cgaaaggctt
     4681 tgtgctggca ttctgagtgt tttgattttt tctccgttca agaagagctc tgcctgcaag
     4741 tttcgactca gggaattgtt gcggatgcgg aaagaaagtt gtttttcatc caggttacct
     4801 tcctcgccga taatttccaa accgggttta agtgccggtg ccagaacctg ccaccaactg
     4861 acatcaccct gtttgagtaa aacaaagaag ctgcgggggc cttcgagtcc gctcacaact
     4921 gcctggaatt gattgtcttt gatttgtact tgttgcagca ccgcctgggg atcgtagata
     4981 tcaagcattt ctgctttgcc gggcagcggt agggacaact cttcgccaat ggccagcaca
     5041 gacgggaaat tccatacttc cctgttgttc ccctgctcga ttatttcaat gtcgaactgc
     5101 cggcccgaag ggcaattaat ttgcaaaagc ggtttttcga ccgaactgat gatatttaca
     5161 tcggccaact ctgttccgtt aatccggacc aaaggaattt ggtcatagcg cagaggcagt
     5221 tccaatacgt aatccaggtc acgaccgaag ccggaatgta tttgccagcg gctggtcttt
     5281 cctttgcgtt cgaaacggta actaaggtcc ggaatttcta tcgaagcctt gtcccattga
     5341 gccgggaagc cgggacgcag atacactttg ttgtgcaaaa gatcgggttc gataccgaac
     5401 agcccttgaa ccattgcgcg tgaataaacg cccaccacat cggcaaaatc gcgatatacc
     5461 tcgccccggg cagcatcgta gtaactgact tgtcccacat tgccggggct gccgcccaga
     5521 tacataccgt ccatgatcgc cgatttcatc agtttgaagc ccatttcagg cctgcctccc
     5581 tggaaacagg ctaaagcggt atgcattatt tcggaaaagg ccacgttgtt gaccgaccag
     5641 acgtaaggtt gccagttgga ggtggaaatc acggccaggt ctgcttcggg catatcattt
     5701 ttagaaagcc ctttggtatt aatttttagg gggatatgag gaatttcgtg ttcaatgtaa
     5761 ttcaaggcct gccagcccgt aaaaggatcg tgcaattccg catcgatggc atggtagatg
     5821 gtccagacgg cggcctgttc gtgcagtctt tgtaaaccaa aggcatcgcg gaattcggcc
     5881 caataccctt tttgagataa ccacaatact ctaccgacag cctctcggat tttatcggct
     5941 tcattatgaa aaggccgtgc atccagaccc aggagttcag ccaattcggc cgccattaga
     6001 tttgcccggt aattgtaggc cgttgaatga gtcacagccc ctccgttgta ctgcagggca
     6061 tcgctggccc agatgcagca ataggcgtcg taaagtccgt catcgtcggg gtcgaagcaa
     6121 cgtttttccc aagccaggtg acgggtcaat accggccaca tttcgcgtac aaattccagg
     6181 tcgccggtcc agcgaaaatg ccagagcagt tcgtctatgt agcagaggtt catgtcgtag
     6241 tggttcatgg tgcccggatt gttgggataa cgggctatgt agcctgaact gtacatttcg
     6301 ctgccggcca ctttggccga gcgggccagg ttgagcgcgg tgtccataat aactcctttg
     6361 tcgggaggaa gagtcatttg tgaggctccg tatgcgcgga aatg
//
LOCUS       DAOSGL010000097         6331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_10812_length_6331_cov_4.395634, whole genome shotgun sequence.
ACCESSION   DAOSGL010000097 DAOSGL010000000
VERSION     DAOSGL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10812_length_6331_cov_4.395634"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1458)
                     /locus_tag="PKY68_04285"
     CDS             complement(13..1458)
                     /locus_tag="PKY68_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH24288.1"
                     /translation="MNKPLISKAFNPDNFRHQGHLLVELLGDYLESALQSSTPVNRWR
                     EPEDQLTFWQNDYKEGDRELGRFFKNLIDSSLHMHNPKNMGHQVAIPAPEAALAGLLT
                     ELLNNGTVVYEIGAPAVVLERVVCKQLAQEMGFDPGKADGFMTSGASLANLTALMAIR
                     NIKAGEDVWNKGNNKQYALMVSEEAHYCIDKSLRIMGLGEAGTIRIPVDEHYKVRVEM
                     LESAYRAARDRGVEVLGIIGCSCSTSTGTYEELEPLADFAQAHNLWFHVDAAHGGPAI
                     FSKKHRHRLKGIERADSVVMDFHKMMMTPALSTAIIFREAKHSYRSFSQKAQYLWEEQ
                     EKKSWFNLGQRSFECTKLMMSVKIYTLLRQYGTALFEEYIDYTYDLAQSFAGMLKQES
                     DLELALQPETNVVCFRYNPGKLSSTSLSKLNSLIRKKLIEDGEFYIVQTQLKDDIWLR
                     VTLMNPFTTTVELRALLEKIKQLGRQLVSEQ"
     gene            1648..2781
                     /locus_tag="PKY68_04290"
     CDS             1648..2781
                     /locus_tag="PKY68_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOH24289.1"
                     /translation="MEYKVLSREYSEIQLATFSPATNNGTREYHALINLKKVSGLNAS
                     VQLDLLEKAYESLKQEISPATAVWKRYFLSDPCNQYTLFDKPDVAISLVGQPPLDGSK
                     MALWCYFVQEGRIDVDSDVITLTRPAYKHFYYYGLNDLSSGSYAQTLKLFNDLKELMG
                     RDKLNMAEHLLRTWVYVQAVDINYEGMVRARREVFEQEGLNKDTHYVSSTGIEGRHYL
                     PSNLVFMDAYAVQGLKKEQQKFLYAPEHLSPTQVYGVTFERGTVVEYGDRKHVIISGT
                     ASIDNQGRTLYPMDIQAQTQRMFENVEALLSEAGAGFGDLVHAIVYLRDIADYELVKS
                     LVNNRLPNVPQVYVLAPVCRSQWLVEMECMAILPVSNKNFAPF"
     gene            2791..4659
                     /locus_tag="PKY68_04295"
     CDS             2791..4659
                     /locus_tag="PKY68_04295"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOH24290.1"
                     /translation="MRNKSSKPGKKQVSRPELPAGTFTWINITGSLLFALYSFWFLGI
                     HKADLMFRLQELTLFLFDKTFFYEHLQSPGGLLEYAGKFLTQFFYYPRLGAVILTALL
                     LLWQWMLVRAFDLPKHLRLLSLLPLSMALLYIGMQEYSIFVAKYSVLSQLAGMILAVL
                     PLFVARFFPGTRARILILLTLVLLGYPFFGFYALLGALLLWVGLFAAGRTETEHQTKL
                     LLRQLLLLLFLLAVPPLFFLLSGQNINPKYLFAYGLPVYDYPNTPWMWTALVACSLLL
                     LGLALLSFVKKDSCRPLWWLNPLFFVLGITFVQAGSYKDLNFETQLAMERAISHEDWD
                     KVLELARKPNDNPNRLLVIYRNIALYNKNQLCERMFTFPNTDEPFHTKNTLVSNTIVG
                     APVNCFYYGLLNYSYRWNMENMVLFGPRPENLRFMMMSALLNREMELAGKYIHILEKT
                     LFYKKWASEHRVYLDDPALLLENKQYKTLLALTQYENLLGQDQRVIELAILNHFCNLK
                     SGTDEMLEWSMSSILTTKSVYKFWPMFFVYVNKNLPLPRHVQEAALLLAYFEKRDLGD
                     LINLMDPRVLQRFDEFKRLTESNTHLTEEMLAALVKRSFEKSYWYYFCFVKDLKTN"
     gene            4661..6157
                     /locus_tag="PKY68_04300"
     CDS             4661..6157
                     /locus_tag="PKY68_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24291.1"
                     /translation="MMSTFLYRLWTVLFVAALMLSCTQTLPNDYAELNEEVTISPDYR
                     NLIIPCNIAPLNFKVEVSAEKILVGIQGDRGGSFVLKGPKVLIPEKKWRSLLEANKSG
                     KYSVEVYARQNGEWNRYQPFTNSIAADSIDEYLSYRLIEPSYVLYENLCIRQRHLGSF
                     WEKDIYNNNLVSEKEDFQCINCHSYQNYQTDNMQFHARAHHAGTLIVCDGVPRKVNLK
                     TEQLISGGVYPAWHPFEKLIAYSVNNTNQLFHSKDIQKVEVLDRNSDLILYDIERNQV
                     SIIQNDSIALETFPAWSPDGKTLYYSSARFESRTGDRESDLATYYQEIKYNIFSVPFD
                     IEQRTFGEPRLLIDAESIGMSALLPRISPDGRYLLFSMAEYGTFHIWHKNSDLYLLDF
                     QNNTIRSLEEVNSKDTESYHSWSSNGRWMVFSTRRDDGSYTRPYIAYFDEQGRAGKPF
                     ILPQKDPDFHLGFLKSYNIPEFMKEPVRVSPRKFARTLKGDAIQAVMR"
     regulatory      6188..>6331
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1572 a         1422 c         1499 g         1838 t
ORIGIN      
        1 gtggcacttc cactattgct ctgacacgag ctgcctgccg agctgtttga ttttctccag
       61 taatgcccgc agttccacgg ttgtggtaaa gggattcatc agggttactc tgagccagat
      121 atcatctttg agctgggttt gcacgatata gaattctccg tcttcgatca acttcttgcg
      181 aatcaggctg tttagtttgc ttaagcttgt gctgctcagc ttgcccgggt tataacgaaa
      241 gcaaaccacg ttggtttcgg gttgcagggc cagttccaga tcgctttctt gtttgagcat
      301 accggcaaaa gactgggcca ggtcgtaggt ataatcgatg tattcttcga acaaagccgt
      361 accgtattgt ctgagcagag tgtatatctt aacactcatc atcagcttgg tgcattcaaa
      421 gcttctttgc ccgagattga accaggattt tttttcttgt tcttcccaca ggtactgtgc
      481 tttttggctg aaactgcggt aagaatgttt tgcttcacgg aaaataatgg ccgtagaaag
      541 ggcaggggtc atcatcattt tgtgaaagtc catcactaca gagtcggctc gttcaatgcc
      601 tttcaaacga tgcctgtgct tttttgagaa tatggcaggg ccgccgtgtg cggcatccac
      661 atggaaccac aggttgtgag cctgtgcaaa atcggcgaga ggttccagtt cttcgtaagt
      721 tcccgtcgaa gtggaacagg agcaaccgat tattcccagc acttcgacac cccggtcgcg
      781 ggctgcccgg taagcgcttt cgagcatttc tacacgcacc ttgtagtgtt cgtccacagg
      841 tatgcgaata gtgcctgctt cgcccaaacc cataatcctc aaagatttat ctatgcaata
      901 atgggcttct tcagacacca tcagggcgta ttgtttgtta tttcccttgt tccagacatc
      961 ttcaccggcc ttgatattgc gtatggccat cagggcggta agattcgcca acgaagctcc
     1021 cgaagtcata aagccgtcgg ccttgccggg atcgaagccc atttcctgag ccagttgttt
     1081 acatacgaca cgctccaaca ctactgccgg agctccgatt tcgtaaacta cggttccatt
     1141 gttgagcagt tcggtcaaca gcccggccag tgcggcttcc ggagcaggaa ttgccacctg
     1201 atgccccatg tttttgggat tgtgcatatg taaagagctg tcaatcagat ttttgaaaaa
     1261 acgtcccagt tctctgtcgc cctctttgta atcgttctgc cagaatgtaa gctgatcttc
     1321 gggttctctc catcgattta caggtgttga actttgcagg gccgattcca ggtaatcccc
     1381 caacaattct accagaagat ggccttgatg tcgaaagtta tcagggttga aagctttgga
     1441 aataagcggt ttattcataa attatctgtt ttttacctaa acccggctgc ccatttgttg
     1501 gggaccggac ttttgttggt gtaaaccctt ggtagggaaa tgacctattt ttggacgatt
     1561 tttgcttgat aagcccacaa aggtagttta taaatcaaaa aatatcctgt atttttgctt
     1621 ttttaaaata acaacaatag aggtaatatg gagtataaag tactctcgag agaatacagc
     1681 gaaatacaat tggccacctt ttcgccggcc acgaacaatg ggacgcgtga ataccatgcc
     1741 ctgatcaatt taaaaaaagt atccggcctg aatgcttcgg ttcagttgga tttacttgag
     1801 aaagcctacg aaagtctgaa gcaggaaatt tctccggcca cagccgtatg gaaaagatat
     1861 tttttgagcg atccctgtaa ccagtatacg cttttcgata agccggacgt agctatatcg
     1921 ctggtagggc agcctccttt ggacggcagc aaaatggcct tgtggtgcta ttttgtacaa
     1981 gaaggccgta tcgatgtgga cagtgacgta attactttga ccaggcctgc ctacaagcat
     2041 ttttattatt acggactgaa cgatttgtcc tcgggctcct acgcccagac cctcaagttg
     2101 tttaacgatt taaaagaact gatgggccgc gataagttga atatggcgga acatttactt
     2161 cgtacctggg tctacgtgca ggcggtggat atcaattacg agggtatggt tagagcccgc
     2221 cgtgaagtct ttgaacagga aggtttgaac aaggacacgc attatgtttc cagtaccggc
     2281 atcgagggca ggcattattt gccttcgaat ctggtgttta tggatgctta tgcggtgcaa
     2341 gggctcaaaa aagaacagca gaaatttctg tatgctccgg agcacctgag tcccacccag
     2401 gtttatggtg ttacatttga gcggggcacg gtcgttgaat acggcgacag aaaacacgtg
     2461 atcatttcgg gtaccgccag catcgacaat caaggccgga ctttgtatcc catggatatt
     2521 caggcacaga ctcagcggat gttcgaaaat gtggaagctt tgttgtcgga ggccggtgcc
     2581 ggatttggag atttggttca tgctattgta tatctgcgtg atatagccga ttacgagttg
     2641 gtaaaatccc tggttaacaa tagattaccc aacgttcccc aagtttatgt gctggcccct
     2701 gtttgtcgca gccaatggtt ggtcgaaatg gaatgtatgg caatcctgcc cgtttcgaac
     2761 aagaattttg ctcctttcta aacagagaat atgcggaaca aaagcagtaa acccggcaaa
     2821 aaacaagtta gccggccgga actcccggcc ggaactttta cttggatcaa tattacaggc
     2881 agtttgctgt ttgctttgta cagcttttgg ttccttggga tacataaggc cgatttgatg
     2941 ttccgcttgc aggaattgac tttgtttttg ttcgacaaga ccttttttta cgagcacctt
     3001 caaagtccgg gaggattgtt ggagtacgcg ggaaagtttc tgactcagtt tttttattat
     3061 ccccggctgg gtgccgttat actgacggct ctgcttttgc tttggcaatg gatgctggtc
     3121 cgggcctttg atttacccaa acatctcagg ctgctgagtc tgctgcctct gtcgatggcc
     3181 ctactttaca tcggcatgca ggaatacagt atttttgtgg ccaaatactc ggttttatcc
     3241 cagttggcgg gtatgatact ggcggtattg cctctttttg ttgctcgttt ttttcccgga
     3301 acccgggcaa gaatcttaat cctgctaacg ttagttctcc tgggctatcc cttcttcggt
     3361 ttctacgctt tattgggagc tttgcttttg tgggtcggat tgtttgctgc cggtcgaacc
     3421 gaaaccgaac atcaaacaaa actcttgttg aggcagttgt tgcttctgct ttttcttctg
     3481 gctgttccgc ctttgttttt tcttttgtcc ggacaaaaca tcaatccgaa gtatcttttt
     3541 gcttacggct tgccggtgta cgattacccc aacactccct ggatgtggac agcgctggtg
     3601 gcttgttctt tgcttttatt gggactggcc ttactttctt ttgtcaaaaa ggactcctgt
     3661 cggccgcttt ggtggcttaa tccgctgttt tttgtactag gtattacgtt tgtgcaggca
     3721 ggttcgtaca aggaccttaa ttttgaaact cagttggcca tggaaagggc catttcgcac
     3781 gaagattggg ataaagtgct ggaactggct cgtaagccca acgataatcc caatcgtttg
     3841 ttggtgattt acagaaatat agccctgtac aataaaaatc agctctgtga aagaatgttc
     3901 accttcccca ataccgatga gcccttccac actaaaaaca ccttggtaag caacaccatt
     3961 gttggggcgc ctgtgaattg tttctattat ggcctgttga attattccta tcgttggaat
     4021 atggaaaata tggttttgtt cggccctcgt ccggagaacc tacggtttat gatgatgtcg
     4081 gccctgctca accgggaaat ggagttggcc gggaaataca ttcacatttt ggagaagacc
     4141 ttgttttaca aaaaatgggc ttcggagcac cgggtgtacc tcgatgatcc ggctcttttg
     4201 ctggagaaca aacaatataa aaccctgctg gctctgactc agtacgaaaa cttgctgggt
     4261 caggaccagc gggtaattga attggccatc ctgaatcatt tctgcaattt gaaatcgggt
     4321 accgacgaaa tgctcgaatg gtcgatgtcg tcgatactga ccaccaaatc ggtgtacaaa
     4381 ttctggccga tgttttttgt ctatgtaaac aaaaacctgc ctttgccccg gcatgtacag
     4441 gaagcagcct tgttgctggc ctatttcgaa aaaagagatc tgggtgatct cataaactta
     4501 atggatcccc gggttttgca gcgttttgat gaatttaaac gccttaccga gtccaatacg
     4561 catttaacag aagagatgtt ggccgccttg gttaaaaggt cttttgaaaa aagctattgg
     4621 tattattttt gttttgttaa agacttaaaa accaactaag atgatgtcaa cgtttttata
     4681 tcgcttatgg acagtgttgt ttgttgcggc tctgatgttg tcctgcactc agactctgcc
     4741 caatgattat gccgaactca acgaagaagt tacaatcagt ccggattaca ggaatttgat
     4801 tattccctgc aatatcgccc ccttgaattt taaagtcgaa gtatctgccg aaaagatatt
     4861 ggtcgggatt cagggagatc ggggtggttc ttttgtgctg aaaggcccca aagtattgat
     4921 tcccgaaaaa aaatggagaa gcctgctcga ggccaacaag tccggaaaat attccgtgga
     4981 agtatatgct cgacaaaacg gagaatggaa tcgctatcaa ccttttacca acagtatagc
     5041 cgccgattcc atcgacgagt acctctctta tcgcctgatt gagccttctt atgttttata
     5101 cgagaatctc tgtatacggc aacggcattt gggcagtttt tgggagaaag acatatacaa
     5161 caacaacctg gtttccgaaa aagaagattt tcaatgcatc aactgccatt cttatcagaa
     5221 ttaccaaacc gacaatatgc agtttcatgc ccgggctcat cacgccggta ccttgattgt
     5281 ctgcgacgga gtaccccgaa aggtaaatct caaaaccgaa caattgattt cgggtggagt
     5341 ttatccggcc tggcatcctt tcgaaaaact gatcgcctat tcggtgaaca ataccaatca
     5401 actgtttcat tccaaggata ttcagaaagt ggaagtgttg gaccggaatt cagatttgat
     5461 cttgtacgat attgaaagga atcaggtaag tatcatacag aacgacagta ttgcgctgga
     5521 aactttccct gcctggtcgc ccgacggcaa gactttgtat tattcttctg cccgctttga
     5581 aagcaggacc ggagaccgtg aaagcgattt ggctacttat taccaggaga taaaatacaa
     5641 tattttttct gtgcccttcg atatcgaaca gcgaactttc ggagagccgc gtttgttgat
     5701 agatgccgaa tccattggaa tgagcgcttt gttgccaagg atctcgcccg acggcaggta
     5761 tctgttgttt tccatggccg aatacggcac tttccacatt tggcacaaaa acagcgatct
     5821 gtacttgctc gattttcaaa ataacaccat acgttcgctc gaagaagtca acagcaagga
     5881 taccgaaagt tatcattcct ggtcgtcgaa cggacgctgg atggtgttca gcacccgcag
     5941 ggacgatggt tcatacacac gtccttacat agcctatttc gatgagcagg gcagggcagg
     6001 aaaacctttt attttgcccc aaaaggatcc cgattttcac ttaggctttt tgaaatccta
     6061 caatattccg gaatttatga aagagcctgt cagggtttct ccccggaaat ttgcccgcac
     6121 cctcaaaggc gatgccattc aggcggtgat gcgttgagta atctttttat cgtaaaacgg
     6181 ggtttttcaa aaaataccct atctttgttg ctgcaaaaag ggaatccggt gtaaatccgg
     6241 aacagtcccg ctgctgtaag gccggagccg ggctcaaccc ggcttttatt ccgctcagga
     6301 ccactgtcta tgcttatgga tgggaaggtc g
//
LOCUS       DAOSGL010000098         6260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_10982_length_6260_cov_4.305882, whole genome shotgun sequence.
ACCESSION   DAOSGL010000098 DAOSGL010000000
VERSION     DAOSGL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10982_length_6260_cov_4.305882"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PKY68_04305"
     CDS             complement(<1..633)
                     /locus_tag="PKY68_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOH24292.1"
                     /translation="MVKYLDLQKITAKYAEEIHEAVARVVDSGWYLQGEENKRFEQHY
                     AEYIGTRYCVGVANGLDALIWILRAYKEMGFMKTGDEVLVPANTYIASILAITDNGLK
                     PVLIEPRLSSYQIDEDLIEKHITERSKALMIVHLYGQCAYSDKIKALCSHYGLKLIED
                     NAQAHGCLYRGRKTGSLGDAAGHSFYPGKNLGAFGDGGAVTTDDEELASVV"
     gene            complement(663..1112)
                     /locus_tag="PKY68_04310"
     CDS             complement(663..1112)
                     /locus_tag="PKY68_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HOH24293.1"
                     /translation="MKNTVYDCSFIELPRIEFRAGNITPVYGNINIPFKIRRVFYTYD
                     IPGGEARGAHAHKECHQFLVAAGGSFEVALDDGINKRTVLLNRPFYGLHIPPGIWASE
                     QGFSSGSVCLVLASTLYDENEYIRDYDEFKAFRKQMETQKSEQASKV"
     gene            complement(1109..1537)
                     /locus_tag="PKY68_04315"
     CDS             complement(1109..1537)
                     /locus_tag="PKY68_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HOH24294.1"
                     /translation="MRLIDLPKILDERGNLSFVEELTQIPFEIERVYWIYDVPGGERR
                     GGHAYRENQEFIIALSGSFDVLLDNGNERKVFSLNRSYYGVYVPKGVWREMNNFSTNS
                     VALILSSTPYSKADYIYNYEEFLQYAAAKTQSGPDKPKQV"
     gene            complement(1594..3159)
                     /locus_tag="PKY68_04320"
     CDS             complement(1594..3159)
                     /locus_tag="PKY68_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOH24295.1"
                     /translation="MNYFVLGLIVLIYLFMLAGISWYAYRNTKNANDYMIGGGNMSST
                     VMALSYGATFISASAIVGFGGVSAAYGMGIQWLCMLNILVGVIIAFIFFGKKTRRLGA
                     ELQAKTFPQLLGSYFQSKSIQVIAATVILLGMPLYAAVVMKGGAVFIEQIFSINYNIA
                     LLIFTLITALYVISGGMKGVMYTSAFQAVVMFVCMFVLLFGLYKALGMGFSEANRALD
                     SIDNLVPSHLASLGHQGWAKMPVLGSPQWYTLVTSLILGVGIGCLAQPQLVVRFLTVR
                     SSKQLNQGIFIGSLFLLITVGVVYHAGPLSNLFFLKHNGQVAVEYISDIDKIIPTFIN
                     EAMPNWFTALFMLSILSASMSTLSSQFHVMGTSAASDIYADVVRKKENRSITSLVKIG
                     VLVSILISYVICYTLKEGVIARGTAIFMGLCAATFLPGYFCMLYWKKTTAKAVLWSML
                     AGAGASLFALLFLHQKEAAAIGLCQALFGRPVLIEAYPWPVIDPILFALPLSIVVIVL
                     LSLLGHKKEQKSV"
     gene            complement(3171..3365)
                     /locus_tag="PKY68_04325"
     CDS             complement(3171..3365)
                     /locus_tag="PKY68_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24296.1"
                     /translation="MFGISDPGIWLAYLLEILCLVFGAWYGIKYWNQDDDIDSSRQAT
                     DILKPKTSKSTKSANKPTHN"
     gene            complement(3376..3804)
                     /locus_tag="PKY68_04330"
     CDS             complement(3376..3804)
                     /locus_tag="PKY68_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOH24297.1"
                     /translation="MTIHQISIFIENKLGSLNEILNIIKSERLQIIASTVSDTVEYGI
                     YRVITPEPRKACLALKSRGLAVNLTEVFAISLANTIGTAAETISYFTLAGINIEYMYS
                     FYLDNRGILIMRTSDTAKTMEVIAKHKLNCLTEEELLQFN"
     gene            complement(3815..5107)
                     /locus_tag="PKY68_04335"
     CDS             complement(3815..5107)
                     /locus_tag="PKY68_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOH24298.1"
                     /translation="MYLHETLETLSRDGIEKLQMERLRKTLNHCLNSPFYRKRFAEHK
                     LRPEDIKTLDDLRKIPFTTKNDLRENYPFGMAAVPLEETVRLHSSSGTTGNPTVVLHT
                     QRDLDQWAEAVARCLYMVGLRKSDVFQNSSGYGMFTGGLGFQYGAERLGMLTVPAAAG
                     NTLRQIKFITDFGTTALHAIPSYASRLYEVMLQQGIDPRDTKLQTLLIGAEPHSEAQR
                     KRIEEMLGVKAYNSFGMSEMCGPGVAFECPEQNGLHIWEDYYIVEIIDPDTLEPVAEG
                     ETGEMVLTTINREAMPLLRYRTRDLTRILPGKCPCGRNHIRLDRMKGRSDDMIILKGV
                     NIFPIQVETILMKFKELSSSYLIVLDTVNNNDEMSVEVELEELFTDDYAKLQGLTKEI
                     TRQLKDELLVTPKVKLVKKDSLPKSEGKAVRVKDLRNH"
     gene            5215..6147
                     /locus_tag="PKY68_04340"
     CDS             5215..6147
                     /locus_tag="PKY68_04340"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH24299.1"
                     /translation="MNKNGRFQPQKQKISTIPVTEDSTLLSFLINTLKDQSRTKIKSL
                     LAHQQVLVNRRIVRQFDHPLKTGDRVNILWTKGKQSLNHPKLKILFEDKYLIVVEKSA
                     GLLSIGTAKERSKTAYSILSDYVKKQHPGNKIFVVHRLDRDTSGLMLFAKNQDIQSLM
                     QTNWRFAVNQRKYTGVLEGVPQTGDGSGQGTVTSYLWESKALIVYASPNPEDGIKAVT
                     HYKILKSSEHYSLAEFKLDTGRKNQIRVQMNSIGYPIAGDLKYGGHVSPIKRMALHAS
                     VLAFTHPVSGEQMLFESETPKEFLRLLELDKKPQ"
BASE COUNT         1797 a         1439 c         1356 g         1668 t
ORIGIN      
        1 caccaccgat gccaactcct cgtcgtcggt ggtaacggct cctccgtcgc caaaagcccc
       61 cagattcttt ccgggataaa aactatgacc ggcggcatca cccaaagaac cggttttgcg
      121 acctctgtag aggcatccgt gagcctgggc attgtcttct atcagtttaa gcccgtaatg
      181 cgagcagagg gccttaattt tgtcgctgta ggcacattgg ccgtagagat gaacgatcat
      241 cagcgctttg ctgcgttcgg taatatgttt ttcgattaaa tcttcgtcaa tctggtaact
      301 cgaaagtctg ggttcgatca aaacaggctt aagtccatta tcggtaattg ccagaatgga
      361 ggcaatgtag gtgttagccg gcaccagaac ttcatcgccg gttttcatga agcccatttc
      421 tttataagct ctcagaatcc aaattaatgc atccaggccg ttagctacac caacacaata
      481 tcgggttcct atgtattcgg cataatgttg ttcaaatcgt ttgttttcct caccctgaag
      541 ataccagccc gaatcgacga cacgagcaac tgcctcgtgt atctcttctg catattttgc
      601 agttatcttc tgcaaatcga ggtattttac catagcccaa ctttaatgct taactgtttt
      661 tctcagactt tcgatgcctg ttcggacttt tgtgtttcca tttgttttct gaaagccttg
      721 aactcatcgt aatctctgat gtattcattt tcatcataaa gagtagaggc cagcaccaaa
      781 caaacggaac cggacgaaaa accctgttcg gaagcccaaa tacccggagg aatatgcagg
      841 ccgtaaaaag gccggttcag cagcacagtt cgcttgttta tcccatcgtc caaagccact
      901 tcaaaactgc cccccgctgc caccagaaac tgatggcatt ccttgtgagc atgagctcca
      961 cgggcttcgc ctccgggaat atcgtaggta taaaaaactc ttctgatttt gaaaggtatg
     1021 tttatattgc cgtaaacagg agtaatgttt ccggctctga attctatcct gggtaattca
     1081 ataaaagaac aatcgtagac tgtgtttttc atacttgctt aggtttatcc gggccggatt
     1141 gagtcttggc ggcagcgtac tgaagaaatt cttcgtagtt ataaatataa tccgctttag
     1201 aatagggagt agaagacaaa atcaaggcca cggaattggt tgaaaaatta ttcatttcgc
     1261 gccaaacccc cttgggaaca tagacaccgt aataagatct gttaagggaa aacaccttac
     1321 gttcgttccc attgtccaag agcacatcaa aacttccgga aagagcgata ataaattcct
     1381 gattttctct ataagcatga ccgccccgtc tttcgccccc gggcacgtcg taaatccagt
     1441 atacccgttc tatttcaaaa gggatctggg ttaattcttc gacgaaagaa aggttacctc
     1501 tttcatccag tattttaggc aaatcaatca atctcatacg cttcttggga ctgttcttgc
     1561 aaatcaacaa taaaatgcct taataaacaa acattaaaca gacttttgct ctttcttgtg
     1621 acccaataaa ctgagcagga caataaccac tattgacaag ggcagggcaa ataagatggg
     1681 atcaatgacc ggccagggat aggcttcaat caataccggt cggccgaaaa gagcctgaca
     1741 caaacctatg gcagccgcct ctttttgatg caaaaacaaa agagcaaaca aactggcgcc
     1801 ggctcctgcc aacatactcc acaacactgc tttggcggtt gtttttttcc aatagagcat
     1861 acaaaaatag ccgggcagga aagtcgctgc acacaaaccc ataaaaatag ccgtaccccg
     1921 ggcaatcacg ccttctttga gggtataaca aatgacgtaa ctgatcagta tggataccag
     1981 aacgcctatt ttaaccaagc tggtaatact tcgattctcc tttttgcgta caacgtcggc
     2041 atagatatcc gaagcggccg aagtccccat aacatggaac tgagaactca gtgttgacat
     2101 actggccgat aaaatactca acataaacag ggccgtaaac caattaggca tggcctcatt
     2161 aataaaagtc gggatgattt tgtcaatatc cgagatgtat tctacggcta cttgtccgtt
     2221 gtgtttcaaa aagaataaat tggataatgg cccggcgtgg tagaccactc ccacggtaat
     2281 taaaagaaat aaagatccga taaaaatgcc ctgattcaat tgtttactgc tgcgtaccgt
     2341 caaaaaacgc accaccagtt gaggctgagc caaacaaccg atgcccacgc ctaaaataag
     2401 tgaagtgacc aaagtgtacc attgaggtga tcccaataca ggcattttgg cccaaccctg
     2461 atgacccagc gaggcgagat gggaaggaac cagattgtct atggaatcga gggctctgtt
     2521 ggcttcggaa aaccccatac ccagcgcttt gtataagccg aacagtaata caaacataca
     2581 aacaaacata actacggcct gaaaagccga agtatacatg acgcctttca ttccaccgct
     2641 gataacgtac aaagctgtaa tcagagtgaa aataagtaag gctatattgt agttaatact
     2701 gaaaatctgt tcgataaata ccgctccgcc cttcataacg acagcagcgt acaagggcat
     2761 gcccagcaaa attaccgtgg cggcaatgac ttgtatggat ttagattgga aatagctgcc
     2821 caatagctgg ggaaaagttt tagcttgaag ttccgctccc aatctgcggg tttttttgcc
     2881 gaaaaagata aaagcaataa taaccccaac caaaatgttt aacatacata gccattgaat
     2941 acccatacca taggcagccg aaacacctcc aaagcccaca atggccgatg ccgaaataaa
     3001 agtagctcca taagataaag ccatcactgt cgagctcata ttgccgccgc ctatcatata
     3061 gtcgttggca tttttggtgt ttctgtaggc ataccagcta atacctgcca acataaacag
     3121 gtaaattaat acgatcagtc ctaatacaaa ataattcata tggcgaagag ttaattgtgg
     3181 gtgggtttat tcgctgattt cgtagatttt gatgtctttg gtttaaggat atcggttgct
     3241 tgccgggaag agtcgatatc gtcgtcctga ttccagtatt ttattccata ccaggcaccg
     3301 aaaacaagac aaagtatttc cagtaaatag gccagccaaa tgccggggtc tgagattcca
     3361 aacataattt gagtattaat taaattgtaa taattcttct tcggtcaggc aattcaactt
     3421 gtgttttgcg atcacttcca tggttttggc agtatcgctg gtgcgcatta taagaatgcc
     3481 tctgttgtcg agataaaaag aatacatgta ttcaatattg atgcccgcca gagtaaaata
     3541 ggagatggtc tcggcggcag taccgattgt attggctaaa gaaatggcaa aaacctcggt
     3601 caggttaacg gccagtccac ggctttttaa tgccaggcag gccttgcgcg gttcgggagt
     3661 aatgaccctg tatatgccat attccactgt gtcggaaacg gtggatgcaa tgatttgcag
     3721 cctttccgat ttgataatgt tcaagatctc gttgagagag cccaacttgt tttcgataaa
     3781 aatggaaatc tgatgaatag tcatggtaca cggattaatg gttacgtaaa tctttgacac
     3841 gaacagcctt cccttcactt ttgggcaggc tgtctttttt gaccagcttc actttgggag
     3901 ttaccagcag ttcgtctttg agttgccggg taatctcttt ggtaaggcct tgcaatttgg
     3961 cgtagtcatc ggtgaacaat tcttcgagtt ctacttcgac actcatttca tcgttgttgt
     4021 tgacagtatc gagcactatc aggtaactag agcttaactc tttgaatttc attaaaatgg
     4081 tttctacctg aatggggaag atgttgaccc ctttcaaaat aatcatatca tcgcttctgc
     4141 ctttcatacg atccaaacgt atatgattcc ggccgcaagg gcatttcccg ggcaggatgc
     4201 gcgtaaggtc gcgggtacgg taacgcagca gaggcatggc ttccctgttg atcgtggtta
     4261 gcaccatttc tccggtttct ccttcggcca ccggttcgag tgtatccgga tcgatgattt
     4321 caacaatata gtagtcttcc cagatatgca ggccgttttg ttccggacat tcaaaagcta
     4381 cgccggggcc gcacatttcg gacattccga agctgttgta tgccttgaca ccaagcattt
     4441 cttctatgcg tttacgctgt gcttccgaat gaggttcggc tccgataagc aaggtctgca
     4501 atttcgtatc acgcggatca atgccctgtt gcaacattac ctcatacaga cggctggcgt
     4561 aacttggtat ggcatgcagg gcggtggttc cgaaatcggt aatgaattta atttgcctaa
     4621 gggtgttgcc tgcagcggcc ggtacggtaa gcatacccag acgttctgct ccgtattgga
     4681 agcccagccc tccggtaaac atgccgtagc cggaggaatt ctgaaaaaca tcggattttc
     4741 gcaaaccaac catgtaaaga catcgcgcca cggcttccgc ccattggtct aaatcccgct
     4801 gagtatgcaa tacaacggtt ggattaccgg tagtaccgct ggaagaatgc aatcttacag
     4861 tttcttccaa aggcactgcc gccataccga aaggatagtt ttcgcgtaga tcgttcttag
     4921 tggtaaatgg aattttcctc aaatcgtcga gtgttttgat atcttcgggc ctgagtttat
     4981 gttcagcaaa acgtttacga taaaatgggg aattcaggca atgattcagg gtttttctga
     5041 gcctttccat ctgtaatttt tcaattccat cccggctcag tgtttccagg gtttcgtgca
     5101 aatacatctt taatacgggt tttatgtcaa aaatgcgcaa atgtaataaa taatctgttc
     5161 tgtaactccc ctggagagta taaagatttc tatctttgcg ttaaaataaa ggatatgaac
     5221 aaaaacgggc gttttcagcc ccaaaaacaa aagatcagta ctattccggt cactgaggat
     5281 tcgactttgc tgagctttct tataaacaca ctaaaagatc aatcccgcac caaaatcaaa
     5341 tctttgctgg ctcatcaaca agtattggtc aatcggcgga tagtccgtca attcgatcat
     5401 ccgctcaaaa ccggcgaccg ggtcaatatt ttgtggacta aaggcaaaca aagcctgaat
     5461 cacccaaaac taaagatctt gttcgaagac aaatacctga ttgttgtgga aaaatctgcc
     5521 ggactgttgt cgataggtac tgccaaagag cgcagtaaga ctgcctattc catactgagc
     5581 gactatgtga aaaaacaaca tcccggcaat aaaatattcg tggtacatcg tctggacaga
     5641 gacacatcgg gcctcatgct ttttgccaaa aatcaggaca ttcagtcttt aatgcaaacc
     5701 aactggcgtt ttgccgtcaa tcagcgcaaa tacacaggag tactggaggg cgttcctcaa
     5761 acgggcgatg gaagcgggca ggggacggtg acttcttact tgtgggaaag caaagctttg
     5821 atcgtatatg ccagtcctaa tccggaagac ggaattaaag ccgtaactca ttataaaata
     5881 ctgaaaagca gcgaacatta cagtttagcc gaatttaaac tcgataccgg aagaaaaaat
     5941 cagatcaggg tacaaatgaa cagtataggt tacccgattg ccggtgattt gaaatacgga
     6001 ggacatgtca gccctatcaa acggatggcg ttgcatgcct ctgtgctggc ttttacccac
     6061 ccggtcagcg gagagcaaat gcttttcgag agcgaaacac ccaaagagtt cttacgccta
     6121 ttggagctgg acaaaaaacc gcaatagttt ctaaaacaac agaagctccg ttatcttgtt
     6181 gatttgcgga gcttctgact gagatgtttg tgcccagaac aggactcgaa cctgcacagc
     6241 ctaacggcca ctagtccctg
//
LOCUS       DAOSGL010000099         6234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11056_length_6234_cov_3.925554, whole genome shotgun sequence.
ACCESSION   DAOSGL010000099 DAOSGL010000000
VERSION     DAOSGL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11056_length_6234_cov_3.925554"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PKY68_04345"
     CDS             complement(<1..976)
                     /locus_tag="PKY68_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24300.1"
                     /translation="MSEQEKNTNKRRISKLLLALVILLVVGWIVNYYVGVVGDSYGKS
                     GLFAQEAPELAHPDISYVFASEPFPAEGKYCLACHQGIEPARPIGSKMMQQILEKGAA
                     LGDPNGCVICHGGTPTELHDKDRAHRGVPKGSLLAAFTPVPAALQVNNNTCGQCHGDH
                     VYNVRLSMMNSDAGKMKAISWSFGIGTENYDHVYANSTLVDTDGETPRFGTGQYIDYM
                     KEMAATFPGQFPSELKEIPAVSAKDIETNPELAAFTYLRNCNACHLSNKGNQGRGHYR
                     GMGCAACHSVYSNEGFYEGGDPEIPKDKPGHLMVHAMQGTRKSSIRFND"
     gene            1280..2419
                     /locus_tag="PKY68_04350"
     CDS             1280..2419
                     /locus_tag="PKY68_04350"
                     /inference="COORDINATES: protein motif:HMM:NF027708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HOH24301.1"
                     /translation="MRTKLFILSSLISFTFLAQAQEISNGVMKLDELRKPAYREIINI
                     PEVNGYQVLKCDFHTHTVFSDGLVWPNIRAQEAWEEGLDAMALTEHIEYHPHKDYVEV
                     NHNRSHELIENLSKRNNVLLIKGTEITRQTPPGHFNAIFIGDASSYIEDNVSEKDWKA
                     VMKAADQNAFIFWNHPGWKPNIEGSYEWLPFLEDLRKKNALHGIEVINGFGFHMKALD
                     WCVDKGLTVMGTSDIHNLVGHDYDRSRKYVHRTMTLVMAKERTPESIREALDAGRTVA
                     WASKYLAGKEENVKNLFNACVKLMPSHYSQEKNGVRTNFYEIQNKSDLYFELELTSGK
                     GTQKITLYPMSSQLLSAEAGQQSLSYDVTNAYVRSDKFLNISFSL"
     gene            2678..3832
                     /locus_tag="PKY68_04355"
     CDS             2678..3832
                     /locus_tag="PKY68_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOH24302.1"
                     /translation="MKKNDFLTLVLILTVFWACLSSSSLFAQSSDGNTSKSVDLSLHL
                     RPAYHAQFDGTPMEFESGGFKFDHVMIDLGGQLTPKLSYRYLQRLNKVSPIFTKENLP
                     STIDYAYLIYAFNERFSMTAGKQALSIGGFEYNKYPVDVYDYSGISNLISCYLTGVQV
                     AFAPTQNQEFSFQIVNNRMGTWQEAIGTPQIGLEAPTLPLYYSLGWNSSYLDNALQLR
                     YAANITSPAKGKTLIMVSGGQKWESGPFSVYIDAFYQHSEIDYLGGIRNLTISPQNVV
                     ENVNYFSLLGEANYRFQPQWNILLKCFYDNFSTYKSSIFLDKGNCLTSWNYQAGIEFY
                     PNKDDNLHLFLIATSANYLKPTVAFIATPDNSFRISAGFIYRVPVLSLKH"
     gene            3848..>6234
                     /gene="ccsA"
                     /locus_tag="PKY68_04360"
     CDS             3848..>6234
                     /gene="ccsA"
                     /locus_tag="PKY68_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOH24303.1"
                     /translation="MEKAKRHLSSYKTTITLLLIYAAVMAVATFIEKDHGTDVAKKWV
                     YYSPAFFLLQFLLILNFIAAWGRQGYVKQKRWALLTVHLAFVVILGGALTTFLFGKEG
                     TVHIREGSKTDEMIIRTADGVSVEKLPFMLELTDFRLNRYPGSASPSSYESDLLVHID
                     GQVQEAKVFMNNVLDVKGYRFFQASYDRDELGTILSVNRDVAGRTITYTGYFLLFCGF
                     LLTFFTKNSRLRRLGRQLKSLREESKHLTLALILLIIATVSQAQTAENTANVAIPQAQ
                     AAESTPNLLIPPEHAAQFAALPVQSDGRIMPMHTLSSEIVRKVYKELKMGQFNPEQFL
                     LSFLAEPQLWIHEPIIAIDNREISSLYGLPKDWASYAQFFDGNGNYKLLQQMQLIYGK
                     LPAERSATDKDMMKLDERVNIIFQLLDYSLLRIYPDPGDETHTWYPPNVSPSIFPKED
                     SLFVADCFRNYLAEVSRSLQSGEWSKPGQLLAEIREFQLKNDIGAHIDVGKIQAEISY
                     NRMRIFDRCKLSYFILGGLMLVLAFMQLLKKRKWTDATIKVLSVAVLCVFLIHAFGMG
                     MRWYIGGYAPWSNSYETMVYVAWATVLAGVVFGRKSTITMALATLFGGIILFVSGLNW
                     MDPQIGTLVPVLKSPWLMFHVAVIVAAYGFFGIGFLLGIVNLSLTAFAKKSELIRFRI
                     KELSITNNMALLVGLTLMTIGTFLGAVWANESWGRYWGWDPKETWALITMIVYSIVTH
                     IHLVKKRDNLWLFNLLSVLAFASDLMTFFGVNYLLSGMHSSGVTDGVSTVFIYIVL"
BASE COUNT         1677 a         1407 c         1428 g         1722 t
ORIGIN      
        1 ggtcgttgaa gcggatgctt gactttctag tgccctgcat ggcgtgtacc atcagatgcc
       61 cgggtttatc tttcggtatt tcgggatcac cgccctcgta aaagccctca ttgctgtaaa
      121 cgctgtggca tgcggcacaa cccattcccc tgtaatgtcc cctgccctga tttcccttgt
      181 tgctcaggtg gcaagcattg cagttacgca aataggtaaa ggcagcaagc tctgggtttg
      241 tctctatatc tttggcagaa acggcaggga tctctttcag ttcggaagga aattgcccgg
      301 ggaaagtggc cgccatctct ttcatataat cgatgtattg cccggtgccg aatcggggag
      361 tctctccgtc ggtatcaaca agcgtgctgt tggcataaac gtgatcgtaa ttttcagtgc
      421 cgatgccgaa gctccaggaa attgccttca tcttgcccgc atcgctgttc atcatggaaa
      481 gacgaacatt ataaacatgg tccccgtgac attgtccgca ggtgttatta tttacttgca
      541 gcgcagcggg aaccggcgta aacgcggcga gcagactgcc tttgggaact cctctgtgtg
      601 ccctgtcttt gtcgtgcaat tcggtagggg ttcctccgtg acagataacg cagccgttgg
      661 gatcgccaag ggctgcacct ttttcgagta tctgctgcat cattttggag ccgatgggac
      721 gggcaggttc aatgccctga tggcaggcaa gacaatactt cccctcagcc ggaaacggct
      781 cacttgcaaa aacatatgaa atatcgggat gcgcaagttc cggagcttcc tgtgcgaaaa
      841 gtccgctttt cccgtacgag tcacctacga caccaacata gtagtttact atccacccga
      901 caacaagcaa aataacaagc gccaataaca acttgcttat tcttcttttg tttgtgtttt
      961 tttcttgctc agacataaag cattcttttt aaaacgacag tgcaatatca taatcttgtt
     1021 ttgattttac aaacgatgtc acgaaaaatc gtttgatcta tggttgtttt ttccacaaaa
     1081 agcattgacg aatgtagtaa aaaagagtgt tgtattagta atttcctttc cggtaattgc
     1141 acggtgcttc gttgacggat gtagtaaaaa cggctactgt attattatgg tttagtatca
     1201 atttaacgct atcgttatta aattgcaaca ataaaatctt tccttgcggc agaaaaacaa
     1261 cctaaaaaca aagtataata tgagaacaaa attatttatt ctatcgtcac ttattagttt
     1321 tactttcctg gctcaggctc aagagatcag taatggagta atgaaattag acgaactgcg
     1381 taaacctgct tacagagaga taataaatat tcccgaagta aatggttatc aagtattgaa
     1441 atgcgatttt catacgcata ccgttttttc cgatggcctg gtatggccta acattcgcgc
     1501 tcaagaagcg tgggaagaag ggctggatgc aatggcttta acagaacaca tcgaatacca
     1561 tccccataaa gattatgtcg aagtaaatca caatcgttcg catgaactca tcgaaaatct
     1621 ttctaaaaga aacaacgttc tcctgataaa gggaacagaa attacacgac aaacacctcc
     1681 cggacatttc aacgccattt tcataggaga tgcctcatct tatatcgaag acaatgtttc
     1741 cgaaaaagac tggaaagcag tgatgaaggc tgctgaccaa aatgctttta ttttctggaa
     1801 tcaccctggt tggaaaccca atattgaagg ctcgtatgag tggctccctt tcttagaaga
     1861 tttacgcaaa aaaaatgcat tgcacgggat tgaagtcatc aacggattcg gattccatat
     1921 gaaagctctc gactggtgtg tagataaggg gttaacagtg atgggtactt ccgatattca
     1981 caacctcgtc ggacatgatt acgatcggag caggaaatat gttcaccgta ccatgaccct
     2041 tgttatggcg aaagagagaa caccggaatc cattcgcgaa gctttagatg ccggaagaac
     2101 cgttgcatgg gcaagtaaat atttggcagg aaaagaagag aatgtgaaaa acctattcaa
     2161 tgcttgcgta aaattaatgc cgagtcatta cagccaggaa aaaaatggag taaggacaaa
     2221 cttttacgaa attcagaata agagcgattt gtacttcgaa ttggaactta catcgggaaa
     2281 aggcactcaa aaaataacgc tttatcctat gtcttcacaa ctcctttctg ctgaagccgg
     2341 tcaacagtcc ctctcttacg atgtaaccaa cgcctacgtc agaagtgata aatttcttaa
     2401 tataagtttc agtttatagg cgtgtctgtg tccgaaccag gcgactgagc ctgggtatcg
     2461 aagccaacag agcagttggg tgtgccggat ttgaccgtag attctgttct atggtatcga
     2521 agccaacaca gcaattgggt agttataaga tactgctttt tatttcggct tttgttttaa
     2581 ctttgcaagt tatatcactg atcgtttgat ttgtggtggc gttttatcgt gaaaatgaaa
     2641 tcgctttatt tgcgtaagtt aaaattttaa aaccggcatg aaaaaaaatg acttccttac
     2701 cctcgtactt attttaaccg tcttttgggc ctgtttaagc agttcgtcgc tattcgcaca
     2761 atcgagcgat ggaaatacat ctaaatcggt tgatctctca ctgcatttac gccccgccta
     2821 tcacgctcaa tttgatggaa caccgatgga atttgaaagt ggtggattta aattcgacca
     2881 cgtaatgatc gatcttgggg ggcagcttac accaaagctc tcgtacagat acttacagcg
     2941 cttgaataag gtttcaccca ttttcactaa ggaaaatttg ccctccacca tcgattatgc
     3001 ttatttgata tatgcgttta acgagcggtt ttcgatgact gccggcaagc aagccctctc
     3061 cataggcggc tttgaataca ataaatatcc cgtagacgta tacgactaca gcggaatatc
     3121 gaatcttatt tcctgttatt taaccggggt gcaggtggca tttgctccca cgcaaaacca
     3181 ggaattttct ttccaaatag tgaacaatcg catgggaacc tggcaagaag ccataggcac
     3241 tccgcaaata ggtcttgaag cgcctaccct gcccctgtac tattcgcttg gctggaacag
     3301 cagctatctg gacaatgccc ttcaacttcg gtatgcggcg aatatcacct cgcccgcaaa
     3361 gggcaaaaca ctgattatgg taagtggtgg acaaaaatgg gaatcagggc ctttcagcgt
     3421 ttacatcgac gccttctatc aacattccga aatcgattat ttgggtggaa tcagaaactt
     3481 gaccatcagc ccacaaaatg tggtagaaaa tgtaaactat ttttctctgt tgggagaagc
     3541 aaattaccgg tttcagcctc aatggaatat tcttttgaaa tgtttttacg acaatttttc
     3601 tacttataaa tcaagcattt ttctggacaa gggtaattgt ctgacctcat ggaattacca
     3661 ggccggtatc gaattttatc caaataaaga cgataatttg cacctgtttt taatcgcaac
     3721 ttctgctaat tacctcaaac ccactgtagc gtttatcgcg actccggaca acagtttcag
     3781 aatttcagcg ggctttattt atcgcgttcc ggtgctctct ttgaagcatt gatcgaggga
     3841 ttattctatg gaaaaagcaa aacgccacct ctcctcatac aaaacaacca tcaccctgct
     3901 gctgatatat gcagcggtaa tggctgtcgc gacatttatt gaaaaagatc atgggaccga
     3961 tgttgcaaaa aagtgggtct attattcgcc cgcttttttc ctgttgcagt ttttgcttat
     4021 actcaatttc attgccgcct ggggcagaca gggctatgta aaacagaagc gatgggcgct
     4081 gttgaccgtg catctggctt ttgttgtgat cttgggcggg gccttgacaa ctttcctttt
     4141 cgggaaagag ggcacagtac acattcggga agggagtaaa accgatgaga tgattatccg
     4201 cactgccgac ggagtcagcg ttgaaaagct gccgttcatg ttggaactga ccgacttccg
     4261 gttaaaccgc tatcccggtt cggcaagccc ctcatcttat gaaagcgacc tgctggtaca
     4321 tattgacggc caagtacagg aggcaaaagt gtttatgaac aacgtgctgg acgtgaaagg
     4381 ctatcggttt ttccaggcct cgtacgaccg ggatgagctc gggacaattc tctccgtaaa
     4441 ccgcgatgtg gcaggcagga ccataaccta cacaggttac tttttacttt tctgcggatt
     4501 tttacttacg ttttttacga aaaactcccg tcttcgccgg ttgggccgtc agcttaagag
     4561 tttgcgggaa gagagcaaac atcttaccct cgctttgata ttgctgatca tagcaactgt
     4621 ttcacaggca caaaccgcag agaatacggc aaatgtcgcg attccacagg cgcaagccgc
     4681 agagagcacc ccgaatctct tgatcccacc ggaacatgcc gcccaatttg cagcattgcc
     4741 cgtacaatcg gacgggagga tcatgccgat gcacaccctc tcatccgaaa tagtacggaa
     4801 agtgtacaaa gagctaaaga tggggcaatt caaccctgaa cagtttctgc tcagcttcct
     4861 tgcagagccg caactgtgga ttcatgagcc gattattgca atagataacc gcgaaatctc
     4921 atcgctctac ggtctgccca aagattgggc ctcttacgca cagttttttg acggaaacgg
     4981 caactataaa ttgcttcagc agatgcaact gatttatggg aaattacctg cagagcgctc
     5041 ggcaaccgat aaagatatga tgaaacttga tgaaagagta aacattatct ttcagctgtt
     5101 ggattacagc ttgctgcgga tttatcccga cccgggcgat gagacgcaca cctggtatcc
     5161 gccgaacgtc agtccctcca tcttcccaaa agaggattct ctgttcgttg ctgactgttt
     5221 caggaactat cttgccgaag tcagccgctc gctgcaaagc ggagagtgga gcaaacccgg
     5281 acaactgctt gccgaaataa gagagtttca attgaaaaac gacatcggag cacatatcga
     5341 tgtaggaaag atacaagctg agatcagcta caacaggatg cgaattttcg accgctgcaa
     5401 gctgtcctac tttattctgg gcggtttgat gcttgttttg gcctttatgc agcttcttaa
     5461 aaaacggaaa tggacggatg caaccataaa agtcttatca gtcgctgttc tgtgcgtttt
     5521 cctgattcat gcttttggta tggggatgcg ttggtatatc ggcggttacg ctccgtggag
     5581 caactcctat gaaacgatgg tgtatgtggc ttgggcaacc gttttggcgg gtgttgtttt
     5641 tggcaggaaa agtaccatca ccatggcatt ggcaacactc ttcggaggga tcattctgtt
     5701 tgtttcgggg ctgaactgga tggacccgca aatagggacc ctggtgcctg tgcttaagtc
     5761 accctggctc atgtttcacg tagcggttat cgtggcggct tacgggtttt tcggcatcgg
     5821 gttcctgttg ggcattgtca acctgtcgct gacggccttt gcaaaaaaga gcgaactgat
     5881 acgcttccgt ataaaagagc tgagcatcac aaacaacatg gctctgctgg tgggtcttac
     5941 gctgatgacc atcggcacct tccttggggc tgtctgggcc aatgagtcat ggggacgcta
     6001 ctggggctgg gaccctaaag agacctgggc gcttataacc atgatagtct atagcatagt
     6061 aacgcatatc catctggtga aaaagaggga taatctgtgg cttttcaacc tcctctcggt
     6121 actggctttt gcatcggatt tgatgacctt tttcggtgtc aactaccttc tgagcggcat
     6181 gcattcctcc ggagtaacag atggcgtttc caccgtcttt atctatatcg tcct
//
LOCUS       DAOSGL010000100         6228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11071_length_6228_cov_4.604892, whole genome shotgun sequence.
ACCESSION   DAOSGL010000100 DAOSGL010000000
VERSION     DAOSGL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11071_length_6228_cov_4.604892"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PKY68_04365"
     CDS             <1..1305
                     /locus_tag="PKY68_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOH24304.1"
                     /translation="KAELFSTFPNDPYFQFQITCHNTGQTFNGHSGTSDADIDAPEAW
                     DITTGNNNIIIAVFDEGVTSDHPDLPNTRQVRLNGSNYGSGDANNPSPTGDDNHGNSC
                     AGVIGATMNNNEGIAGIAPNCRIMPLRWDATTTSDEMADGIEFAVDNGANIISNSWGY
                     STSNNNYIPAIVTAIQYAINNNVVVVFAAGNTARHNSCCDNGYVTFPANANVNGLLTV
                     GASDRFDNQADYSPTSSLIDIVAPSHRAYPPEAYSPSCGGISGETFEMWTLDIPGDAG
                     YNTWPSTGIHPPATGETLPNTGTNNLSYTGRFGGTSHACPVVAGVAALLSENPNLTPQ
                     EVFNTLTNTADKIGGYTYTDGRCNQTGFGRVNAFNALQALCTTTNFVNQTVTTNTTVE
                     DCNVNVQNVDVTNNSKLTIEAENEVIINGEFEVELGSELEIK"
     gene            1456..1689
                     /locus_tag="PKY68_04370"
     CDS             1456..1689
                     /locus_tag="PKY68_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24305.1"
                     /translation="MKQSANLKHIPKMKIAFQIQLIVSAVFFFGLGCTSDDPKPASKD
                     KITGTWIEKNPELFDGVSDTIVFTGLFITSSTG"
     gene            complement(1765..4182)
                     /locus_tag="PKY68_04375"
     CDS             complement(1765..4182)
                     /locus_tag="PKY68_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOH24306.1"
                     /translation="MKTKLSGLLALLLISSGVYAQAYQKTTLGIKTQVKEHEIELQFY
                     SPDIVRVVKYPAGHSFDKQSLSVVLEPGKTPFNVKQQGDELVLDSKKMQVRLDLNSGK
                     IAFSAEGKSLLSEKEAGASFSEFNDAGTKTFSVKQSFVLDQDETIYGLGQQQDARMSK
                     RNVRLNMIQGNTDDYVPFLLSIKAYGLFWDNYSPTVFEDNAAGCYFDSQVGECIDYYF
                     MYGGHADGVIARMRELTGQVPMFPLWTYGFWQSRERYKSQEELLTVVRKYRELEVPLD
                     GIIQDWQYWGNNYLWNAMDFLNPNFPNPQGMVDDVHQLNAHIIISIWNSFGPQTKQYK
                     ELEKMNALFDMTTWPPYGPERHPVNQEYPSGVKVYDVYNPKARELYWRYLDKGLFSLG
                     MDGFWIDSSEPDHLNFKPSDFDNQTYLGSFRKVRNAFPLMAVGGVAEQQRAKSSDKRV
                     FILTRSAFAGQQRYGANLWSGDVSASWQALHAQITAGLNVSLSGIPHWNSDIGGFFLW
                     RFRNPLNNPDYRELHVRWMQFGCFCPMMRSHGEGSPREIYQFGQKGNPIYDALEKYIQ
                     LRYRLLPYIYSTSYEVSALQSSMMRALMMDFPQDPAVLDLNSEYMFGKALLVCPVTEP
                     MYTTYVPRGRDSVLREDFSKVKTKEVYLPAGTAWYDFWTGEKMDGGRKVGKEVPLDIM
                     PLYVRAGSILPWGPKVQYATEQNWDQLEIRVYPGADGEFVLYEDEFDNYNYENGAYST
                     ISFKWDERQKALTIGARQGNFPDMLLNRSFRVVFVEGSKGSGPDSSEHYDQVVKYSGK
                     KVVVRKR"
     gene            complement(4179..5078)
                     /locus_tag="PKY68_04380"
     CDS             complement(4179..5078)
                     /locus_tag="PKY68_04380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH24307.1"
                     /translation="MISICIPVYNFDIERLIKALSSQIESVDAVVELIVIDDGSEADI
                     KTGNKTTCQNCKYVELPENLGRSKIRNLFLNYATQDYLLFLDCDSLIENPRFLSNYLE
                     ILKQQPKVVCGGSTYRPQRPGRQQRLRWKYGIYKESRSAEIRSEQPYNSFMSNNFLIQ
                     RRVFKEVRFDERITKYGHEDTLFGYSLKKKQLPITHIDNPALNGEIDTNSEFLRKTRE
                     GIVNLISILNFEEYHQDLMEGISLLRFYQKIRKFESIVLWLFLLTKPLISCLLSKGYV
                     SLKLFDFYKLGLFIEQKRKISKT"
     gene            complement(5354..>6228)
                     /gene="dprA"
                     /locus_tag="PKY68_04385"
     CDS             complement(5354..>6228)
                     /gene="dprA"
                     /locus_tag="PKY68_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOH24308.1"
                     /translation="WFEDEDYPARLRTCPDAPLLLYSMGEAELNPSKILSIVGTRKMT
                     AYGRMFCEQLVQDLAGAFPDLTIVSGLAYGVDICAHKAALDFNLKTLAVLAHGLDRIY
                     PPAHRQTAKKIVHQGALLTEMPSFTQPLGWRFVQRNRIVAGLSDACVVVESAEKGGSL
                     ITAYMAREYNREVFALPGRTIDPFSRGCNKLIKNQVAVLLEKAEDLIREMNWDRNVKQ
                     QSRQQTLFPLLDGAMQEVFDLFAEGRDYDTSELYELLRQKRDCDMGYLLSILLQLELY
                     CLIRALPGNKYRLY"
BASE COUNT         1719 a         1419 c         1375 g         1715 t
ORIGIN      
        1 aaagccgaat tgtttagcac atttccaaat gacccgtatt ttcagtttca aattacttgt
       61 cacaacacag gtcaaacatt taatgggcat agcggaacat ctgatgctga tattgatgca
      121 cctgaagcat gggatattac aacggggaat aacaatatta taattgcagt ttttgacgaa
      181 ggagttactt cagaccatcc cgatttacct aatacaagac aagtaaggct aaatggtagt
      241 aattatggtt caggtgatgc aaataatcca tcaccaacag gagacgataa tcatggaaat
      301 tcctgtgctg gtgttatcgg ggctactatg aataataatg aaggaattgc aggaattgcg
      361 ccaaattgta gaattatgcc tttaagatgg gatgctacta caacttctga tgaaatggca
      421 gacggcattg agtttgctgt ggataatgga gcaaacatca tttctaatag ttggggttat
      481 agtacatcta ataataacta tatacctgca attgtaacgg ctattcaata cgccataaac
      541 aataatgtgg tagttgtttt tgctgctggt aatacagctc gtcataattc ttgctgtgac
      601 aatggttatg ttacattccc tgcaaatgca aatgtaaatg gtcttctgac agttggtgct
      661 tcggatagat ttgacaatca agccgattat agtcctacaa gctcactaat agatatagtt
      721 gccccctctc acagagcata tcccccagag gcttattccc ctagctgcgg tggtattagc
      781 ggcgaaactt ttgaaatgtg gactttagat attccaggag atgctggata taatacatgg
      841 ccatcaacag gtattcatcc gcctgcaact ggtgaaacat tgcccaatac aggaacaaat
      901 aatctttcat atacaggtcg ttttggagga acttctcatg cttgccccgt tgttgccggt
      961 gtagctgcat tattatcaga aaaccccaac ttaacgcccc aagaagtttt taacacttta
     1021 acaaatacag cagacaagat aggaggatat acatataccg atggtagatg caatcaaaca
     1081 ggttttggta gagtaaatgc ttttaatgca ttacaagctt tatgtactac caccaatttt
     1141 gttaatcaaa ctgtaactac aaatactacg gttgaagatt gtaatgtaaa tgttcaaaat
     1201 gttgatgtaa caaataactc aaaattaaca attgaggctg agaatgaggt gataataaat
     1261 ggagaatttg aagttgaatt aggctctgaa ttggagataa aataagtact ggctctaatg
     1321 cgtcatacgt aatggcgggg caagtagtat atatcaaggt ttgcggctcg catcaacacc
     1381 atctcggata gagaaaagct ccgaagtccg ccactacgca tacacccccg taaacatcgt
     1441 tcatagccaa aagcaatgaa acaaagtgct aatcttaaac atataccaaa aatgaaaatc
     1501 gcgtttcaaa tccaactgat tgtaagtgca gtattctttt ttggtttggg atgtacaagc
     1561 gatgatccta agcctgcatc aaaagacaaa attaccggaa cgtggataga aaagaatccc
     1621 gaattattcg acggcgtctc cgacaccatc gtcttcacag ggttatttat aacttcatcg
     1681 acaggctgat aacttcccag gtaaaagaca ttccatttat taaaattgaa tgatctagta
     1741 tccatcgatg atatcaacga acctctatct ttttctgaca actacttttt tgccgctgta
     1801 ctttaccacc tgatcgtagt gttcgcttga gtcggggccg ctgcctttgc tgccttcaac
     1861 aaaaacgacg cgaaaactgc ggttcaaaag catatcgggg aaatttccct gcctggctcc
     1921 gatggtcagg gctttttgtc tttcgtccca tttaaagctg atggtggaat aagccccgtt
     1981 ttcgtaattg taattatcga attcgtcttc gtacaacaca aattcaccgt ctgcccctgg
     2041 gtaaacacga atctcgagct gatcccagtt ttgttcggtt gcgtattgca ctttggggcc
     2101 ccagggcaaa atggaacctg cccgcacgta gagaggcatg atgtctaaag gaacttcttt
     2161 gccgacctta cggcctccgt ccattttttc gccggtccaa aagtcgtacc aagccgtacc
     2221 cgccggcaga tatacttctt tggtttttac tttgctgaaa tcttctctca gcaccgaatc
     2281 ccggcctctg ggtacataag ttgtgtacat aggctcggtg acgggacaaa ccaaaagggc
     2341 cttgccaaac atatattcgc tgttcaaatc cagcacggcg ggatcctgcg ggaaatccat
     2401 catcagggcg cgcatcatgc tcgactgcaa ggcactcact tcgtaggaag tggaataaat
     2461 ataaggcagc aggcgataac gcaactgaat gtatttttcc agggcatcgt agatgggatt
     2521 ccctttttga ccaaattgat agatctcgcg gggagagcct tctccatgag aacgcatcat
     2581 ggggcaaaaa cagccaaact gcatccagcg cacatgcaat tcccgataat cgggattgtt
     2641 taagggattc ctgaatctcc acaggaaaaa cccgccgata tcgctgttcc agtgggggat
     2701 tcccgacaat gaaacattca ggcccgccgt gatttgagcg tgtaaagcct gccaagaggc
     2761 cgatacgtcg cccgaccaaa gattggctcc gtagcgctgc tggcctgcaa aagcagaacg
     2821 ggtcagtata aatacgcgct tgtcggacga cttagccctt tgctgttcgg ccacaccacc
     2881 cactgccatc agaggaaaag cgttgcgtac tttgcggaaa gaacccagat aggtttggtt
     2941 atcgaaatcc gaaggcttga aattcagatg atcgggctcg gaagaatcga tccaaaagcc
     3001 gtccataccc agagaaaaca gccctttgtc caaatacctc caatacaatt ctcttgcctt
     3061 gggattgtag acatcgtaca cctttactcc cgaaggatat tcctgattga ccggatgtct
     3121 ttcgggaccg tagggaggcc aggtggtcat atcaaaaagg gcattcattt tttcgagttc
     3181 tttgtattgt ttggtctgcg gaccaaagga attccaaatg gaaataataa tatgagcatt
     3241 gagttgatgc acatcgtcaa ccattccttg cgggttagga aagttggggt tcaaaaaatc
     3301 catggcattc cacaaataat tgttgcccca atactgccaa tcctgaataa taccgtccag
     3361 gggcacttcg agttcccggt atttacgcac taccgtcagc aattcttcct gacttttgta
     3421 gcgttcgcgg ctttgccaaa aaccgtaagt ccacaaaggg aacatgggaa cttgcccggt
     3481 gagttcgcgc atacgggcga ttactccgtc ggcatgaccg ccgtacataa aataataatc
     3541 gatgcactct cctacctgcg aatcgaaata gcagcccgca gcattgtctt cgaacaccgt
     3601 gggggaatag ttatcccaaa acaaaccgta ggcttttatg gacagcaaga aaggaacata
     3661 gtcgtcggta ttgccctgta tcatattcaa cctgacgttg cgtttggaca tcctggcgtc
     3721 ctgttgctgt cccaggccgt aaatagtctc gtcttggtcg aggacaaaag actgttttac
     3781 cgaaaaggtc ttagtgccgg cgtcgttgaa ttcgctaaag gaggctccgg cttctttttc
     3841 gctcagcaaa gattttcctt cggcgctaaa agcaattttg ccgctgttca gatcgagccg
     3901 gacctgcatt tttttgctgt ccaatacgag ctcatcgcct tgctgtttga cgttgaacgg
     3961 agtttttccg ggttcgagca ccaccgacaa actttgcttg tcgaaactat gccccgccgg
     4021 gtattttacc acacgaacaa tgtcggggct gtaaaactgc agttcaattt cgtgctcctt
     4081 gacttgggtt ttaataccca atgttgtttt ttgataagct tgagcataga ctcctgagct
     4141 gataagaagt aaggcaagca agcctgatag tttggttttc atgtctttga tatttttctt
     4201 ttttgttcaa taaataggcc gagtttataa aagtcgaata gtttcaaact cacatatcct
     4261 ttgcttaata agcaacttat caatggtttc gtcagtaaga aaagccataa aacgatgctt
     4321 tcaaacttcc ggatcttttg gtaaaaccgc aaaaggctga tgccctccat caggtcctga
     4381 tggtattctt caaaattcaa aatggaaatc agattgacaa tgccttcgcg ggttttcctt
     4441 agaaattcag agttggtatc gatttctccg ttcagcgccg gattgtcgat atgggtaatc
     4501 ggcagttgtt ttttctttag ggaatagcca aataaggtat cctcgtgccc gtattttgta
     4561 atgcgttcgt cgaacctgac ttctttgaac actctgcgct gaatcagaaa attgttcgac
     4621 ataaaagagt tgtaaggctg ctcactgcga atctcagcag accggctctc tttgtaaatt
     4681 ccgtatttcc agcgaagtct ttgttgtctg cccggacgct gggggcgata tgtactgcct
     4741 ccgcacacaa ctttgggttg ctgttttagt atttccaggt aattacttaa gaaccgcgga
     4801 ttttcgatga gagaatcgca atccaaaaag agcaggtaat cctgagtggc gtaattcaaa
     4861 aatagatttc tgattttgga ccgccctagg ttttcgggca attctacgta cttgcaattt
     4921 tggcaagtgg ttttgttccc ggtttttatg tccgcctcgg agccgtcgtc aatgacaatg
     4981 agctccacta ccgcatccac cgactcaatt tgagaagata aagctttgat taatcgttct
     5041 atatcaaaat tatataccgg aatacaaatg ctgatcatgg atgcagattt cgctcagtaa
     5101 agctgtttgt ctgccaaatt tagtttttat tattgacatt acttgcaatt gtatacagtc
     5161 attgtgattt ttttgccttt gagcttaatt tacagaaggt aaacctgtcg gctcaccctc
     5221 gcccggcagc attattctgt cggttgattc catttgcatc cacatgaatc gatgactgta
     5281 atttcggtta attgattttt taacagggct tcccgtcgat ctaccgacag gactcgaaag
     5341 gatggagagc cggtcaatac aagcggtatt tattacccgg tagagcgcga atcaaacagt
     5401 ataattcgag ttgcaacaag atcgacagca aataacccat gtcgcaatcg cgcttctgcc
     5461 gcaagagttc atacaattcg gaagtatcgt aatccctgcc ctcggcaaat aaatcgaaca
     5521 cctcttgcat agcgccatcc aacaagggga acaaggtttg ctgacggctt tgttgcttga
     5581 cgttccggtc ccagttcatc tcccgaatca gatcttcggc tttttccagc aaaaccgcca
     5641 cttgattttt gatcaactta ttgcaacccc gcgaaaaagg atcgatggtc cggccgggca
     5701 aggcaaaaac ttcgcggttg tattctctgg ccatataggc agtgatcaaa gagccccctt
     5761 tttcggccga ttccactacc acacaggcgt ccgacaagcc cgccactatg cgattgcgct
     5821 gtacaaaacg ccaaccgaga ggctgggtga acgagggcat ctccgtgagc agagcaccct
     5881 gatgcacaat ctttttggcc gtttgcctgt gtgcgggagg ataaatacga tccaggccgt
     5941 gagccagcac agccagggtt ttcaggttga aatccagggc cgctttgtgt gcacatatat
     6001 ccaccccgta tgcgagaccg ctcacaatgg taagatcggg gaaagcgccg gccaaatcct
     6061 gaacaagctg ttcgcaaaac atacgcccgt aggctgtcat cttgcgggta cccactatcg
     6121 aaagtatttt ggagggattc aattcggctt cgcccatgct gtacagcaac aagggagcat
     6181 cggggcaagt gcgcaaacgg gctggataat cttcgtcctc aaaccaat
//
LOCUS       DAOSGL010000101         6187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11157_length_6187_cov_3.454175, whole genome shotgun sequence.
ACCESSION   DAOSGL010000101 DAOSGL010000000
VERSION     DAOSGL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11157_length_6187_cov_3.454175"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="PKY68_04390"
     CDS             <1..1452
                     /locus_tag="PKY68_04390"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOH24309.1"
                     /translation="TAKAEITRLYGEDNVKTRQYQTKSKGAQEAHEAIRPTYMSVHSV
                     DASGPEKKLYELIWQRSIASQMADARLEKTTITISHSNGNQLFTASGEVVTFEGFLKV
                     YEYPDKENEAENTGSKLLPSVQLQQTLNLDTAQAVQRFSQKPPRYTEASLVRKLEELG
                     IGRPSTYAPTISTIQQREYIEKGNREGEKRQYNLLELSKTRQINDKTKTEMSGVEKAK
                     LFPTDVGMVVNDFLLESFPYIMNYQFTANVEKYFDQIAEGELEWVELIDQFYKDFHPA
                     VQEATAYSGPKVGSRLLGKDPKSGRPVSVRIGRFGPLVQIGTAEDEEKPLFASLRKEQ
                     SMDSINLEEALALFKLPRELGSFEGKSLSVGSGRYGNYVRHDNQFVSLPKEFDPLEVS
                     LEQAIQLVKDKREEDRKKIILSFEQDPELLVLNGRYGPYISKKGQNYKIPKKTDPATL
                     SYQDCLDLIEKQAKETNIKKGRKMIRKSPPKKK"
     gene            1462..1836
                     /locus_tag="PKY68_04395"
     CDS             1462..1836
                     /locus_tag="PKY68_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24310.1"
                     /translation="MKKSQHKTSLFFWTIFFVSYVSTSCITESEYDSSLLIGKWVSGT
                     EYYRYNEDGTGLTWDTADDVTEQEAQAFTWTLVYSTLTHMHILEVGGVVPKVYTVTEL
                     TATNLKYEDEFGKTYSFTKSSS"
     gene            1868..5083
                     /locus_tag="PKY68_04400"
     CDS             1868..5083
                     /locus_tag="PKY68_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOH24311.1"
                     /translation="MKKTHPIIIRILACIIGLPVLVFIVVLVLLLHPKSLHSMVQTQA
                     EKYLNCSLEIGSIDLDLFDNFPQMELSIKELSLLNPFPGAPNDTLLGLKQIKASVHLG
                     KLLTQRALVIDTFLIDRLNFYAYIDSNGKSNFAVLNLESDPEDTTALTLPFESVQLGF
                     FELQEFGIMYVDEADSILALANGLNLDLNLKYREQMAEGMLCLSSPDLSLEMDGASYL
                     DRDSLKMELPFYFEDNKLNLTKALLALNHFRLQSSGSIALHDQGLLFDLGFETAQAAI
                     QPSLQLLPPVADALPEGVFVDGLFGFQGKIKGLYNDSLMPEILLFGTLKNGFFSYDDP
                     TVELKDLSSEVELFVDMNHKKASYVVLREAKAHTGKSSLEGGIFINDLLGDDMHIDLD
                     MALDLDLQETAGFLPEDMDISLIGRAKGPGKARFFLSELMELNLQQMHMQTDLLLQNF
                     KGRYRDYRLETSRGRLNLTIPKDNSLTSLLNAHLYQCPLLSAGMDSSLKAELQQAKVY
                     FELSDVMSTTDSVAFSSKLKAEKALVWYNQIAADIVNPKAEIAMQMDFVDSTAVSDLH
                     FDVEAEQLYGQMDSVRVNIKNPVARLQMKGQEEEKSRAFFKVDYSSSALNLRYGQEVV
                     KTESLGIRAEAYEDNTKEQLLLRWNPKLEVYLQEGDIELTAISEKIFIPDIRFNYSNE
                     VFSIIDSRIQLGNSDFQLMGNVLHIGDYLNNTGSLKAELNFVSNLTDVNQLMKLTSGL
                     GETENEENAGTEAAQAYNTGSSGKEVAQAHNTGGSEQEEAQAHNTGSSGGEATPADST
                     VASGPFLVPKNIDLNVNTSIMNAYVGNQIATNLTGRLYVKDDALVLEEIGFVSAAARL
                     KLTALYRSPRPNHLYLGLDYHMVDIQIDELLSMFPDLDTIMPMLSSFKGQGEFHLAAE
                     TYLDKNYNLKKSTLRGASSIRGDNLVLMDSETFGKISKILMFNKKTENKIDSLSAEFT
                     VFKNEVDVYPFTIVMDKYKAVVGGRHNLDMSFDYHISLLESPLPMRLGVNISGTMDDL
                     KIRPAACKYPENYRPVSRRIVDAQKLELRKLIRETLIKQME"
     gene            complement(5133..6167)
                     /gene="rhaT"
                     /locus_tag="PKY68_04405"
     CDS             complement(5133..6167)
                     /gene="rhaT"
                     /locus_tag="PKY68_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOH24312.1"
                     /translation="MNTLIGLLVIAVGSFGQSSSYVPINKIKNWSWESFWLVQGVFAW
                     LIFPFLGALLAVPQGSTLGALLSVDPAASWKAIGFGALWGVGGLTFGLSMRYLGVALG
                     QSIALGTCSAFGTLIPALFFDKQDLLHGDGLILLLGVCITLVGIAVIGYAGGLRAQNM
                     TEEQKKAAIKDFALTKGLLVALLAGVMSACFALGLSAGQPIADAAKSVGANDLFATLP
                     TTLMVTLGGFFTNAVYCLSQNAKNKSFKDYTSNSRKIWVNNLFFCSLAGLLWYSQFFG
                     LSVGKSFLAENAIMLAFSWSILMALNVTFSNVWGILLKEWKGVSTKTIVVLILGLFIL
                     VFSVFFPNLF"
BASE COUNT         1873 a         1520 c         1404 g         1390 t
ORIGIN      
        1 accgccaaag ccgaaatcac ccgcctgtac ggtgaagaca acgtcaaaac caggcaatac
       61 cagaccaaaa gcaaaggtgc ccaggaagcg cacgaagcca tacgtccgac ctatatgagt
      121 gtacacagcg ttgatgcctc cgggcccgaa aagaaattat atgaactcat ttggcaaagg
      181 agcatcgcct ctcaaatggc agatgcacgt ctggaaaaaa ccaccattac cattagtcat
      241 tccaatggaa atcagttgtt caccgccagc ggagaagttg tcactttcga aggttttctg
      301 aaggtctacg aatatcccga caaagaaaat gaagccgaaa acaccggcag caaattgctg
      361 ccctctgttc agctccaaca aacactgaat ttggatacgg cccaggctgt tcaacgtttc
      421 agccaaaaac cgccacgcta taccgaagcc tctttggttc gtaaactcga agaattgggc
      481 atcggaaggc cttcgaccta cgctccgact atttccacca tccaacaacg ggaatacatc
      541 gaaaaaggca accgggaagg tgaaaaacgc cagtacaacc tgctggagct gtctaaaaca
      601 agacaaatca acgataagac caaaaccgaa atgagcggag tagaaaaagc caaactcttt
      661 cccaccgatg tcggtatggt ggtcaacgat tttcttctgg aatctttccc ctacattatg
      721 aactaccaat ttacggccaa tgtggaaaaa tatttcgacc agattgccga gggcgaattg
      781 gagtgggtag aattgataga ccaattttac aaagatttcc atccggcagt gcaggaagcc
      841 actgcttatt ccggacccaa agtgggcagc cggcttttag gtaaagatcc taaaagcggc
      901 cgtccggtat cggtacgcat aggtcgtttc ggacccctgg tacaaatagg cacggccgaa
      961 gacgaagaaa aacctttgtt tgcctccctg cgaaaagagc aaagcatgga cagtatcaac
     1021 ctggaagaag ctctggcttt gtttaagctt ccccgcgaac tgggatcttt cgagggtaaa
     1081 agtctaagcg tgggcagcgg acgttacggc aattatgtac ggcatgataa tcaatttgtt
     1141 tccctgccca aagaattcga tcctctcgaa gtgagtttgg agcaggccat ccaactggtt
     1201 aaagacaaac gggaagagga ccggaaaaaa atcattctca gctttgaaca ggatccggaa
     1261 ttattggttc tcaatggtcg ctacggacct tacataagca aaaaaggaca gaattataaa
     1321 atccccaaaa aaacagaccc ggctacactc agctaccaag actgtctcga tcttatcgaa
     1381 aaacaagcca aagaaaccaa tataaaaaag gggcgcaaaa tgatcagaaa aagcccaccc
     1441 aaaaagaaat aaacctgcgc tatgaaaaaa tcacaacaca aaacaagctt atttttttgg
     1501 accatttttt ttgtcagcta cgtatcgact tcctgtatta cggaatcaga atacgattct
     1561 tcactgctga taggtaaatg ggtttccggt accgaatact accgctacaa tgaggatggt
     1621 acaggactaa cctgggacac agccgacgac gtaacagaac aagaggctca ggcatttacc
     1681 tggactttgg tctactccac cctcactcat atgcacattt tggaggttgg aggagtggtg
     1741 cccaaagtat atacagttac cgaacttacg gccaccaact taaaatacga ggacgagttt
     1801 ggtaaaactt acagttttac caaaagcagc tcctaacagg agctttccga gacttagtac
     1861 ctgatttatg aaaaagaccc acccaataat aatcaggata ctggcatgca tcatcggttt
     1921 gccggtgctg gtttttattg tggtactggt cttgttgttg catcccaaaa gccttcattc
     1981 catggtacaa actcaggcag aaaaatacct gaactgttcc ttagaaatag gaagcatcga
     2041 cctggatctg tttgacaact tcccccagat ggaacttagc atcaaggaac taagtctgct
     2101 caatcccttt cccggtgctc ccaacgatac tctgctggga cttaaacaaa taaaggcttc
     2161 cgtacatttg gggaaattac tcacacagcg ggcactggtt attgatactt ttctcatcga
     2221 ccgcctgaac ttctacgctt acattgacag caacggaaag tccaattttg ctgtccttaa
     2281 tctggaaagc gatcccgaag ataccacagc cctgactctg ccttttgaat ctgttcagct
     2341 gggttttttc gagctgcagg aattcggaat tatgtatgtt gatgaggccg acagcatact
     2401 tgccctggca aacgggctga acctcgacct gaatctgaag taccgggagc aaatggccga
     2461 gggaatgctc tgcctgtcca gccccgacct gagcctggaa atggacggag caagctacct
     2521 cgacagggac agcctgaaaa tggaacttcc gttttatttc gaagataata agttaaacct
     2581 gacaaaagct ttgctggctc ttaaccattt ccgccttcaa agcagcggtt caattgcact
     2641 gcatgatcag gggcttttgt ttgacctggg ttttgaaaca gcacaggcag ccattcaacc
     2701 aagcctgcaa ttattgcctc cggttgcgga cgccttgccg gagggcgtct tcgtggacgg
     2761 gctcttcgga tttcaaggca aaataaaggg tttgtacaac gacagcctaa tgcccgaaat
     2821 tctattgttt ggcactttga agaacggttt tttcagctac gatgatccta ccgttgaatt
     2881 aaaggatctt tcttccgagg tagagctgtt tgttgatatg aaccataaaa aggcttctta
     2941 tgttgtgctt cgggaagcaa aagctcatac cggaaaatca agtttggagg gtggaatttt
     3001 tatcaatgat ttgctgggag acgatatgca tattgatctc gatatggccc tggatcttga
     3061 cctgcaggaa acagcaggct tcttgcccga agatatggac atcagcctga ttggcagggc
     3121 aaaaggtcca ggtaaagctc ggttttttct ttcagaactg atggaattga atctgcagca
     3181 aatgcatatg cagaccgatt tgcttttgca aaattttaaa ggacgctacc gggattatag
     3241 gctggaaacg tcccgtggca gactgaatct aactattccc aaggacaaca gtctcacctc
     3301 gttgctcaac gcccatctct accaatgccc tttattatct gccggcatgg acagcagcct
     3361 gaaagcggag ctgcaacagg ccaaggttta tttcgaactc tccgatgtga tgagcactac
     3421 cgacagtgtt gccttctcaa gcaaactgaa agccgaaaag gccttggttt ggtacaatca
     3481 aatagcggcg gacatagtca atccaaaagc agaaattgcc atgcagatgg attttgttga
     3541 cagtaccgct gtttctgatc tccatttcga cgtggaagcc gagcagcttt acggtcaaat
     3601 ggacagtgtc cgggtgaata tcaaaaaccc ggtggccagg ctgcaaatga aaggccagga
     3661 ggaggaaaaa agcagagcct ttttcaaggt cgattactcc agctctgccc taaacctgcg
     3721 ttacggacag gaagtcgtca aaaccgaatc cctcggaatc agagcagagg catacgaaga
     3781 caatactaag gagcagcttt tgttgcgttg gaatcccaaa ctggaagtct acttgcagga
     3841 aggcgacatt gaattgaccg ccatctcgga gaaaattttt atccccgaca ttcgcttcaa
     3901 ctacagcaac gaagtcttca gtattatcga cagccggatc caactgggca attccgattt
     3961 tcaacttatg ggcaatgtgc tgcatatcgg cgattacctg aataataccg gcagcctcaa
     4021 ggcagaactc aacttcgttt ccaaccttac cgacgtcaat cagttgatga agctcaccag
     4081 cggactggga gaaacggaaa acgaagaaaa cgccggaaca gaagcagcac aagcgtacaa
     4141 tacgggaagt agcggaaaag aagtagcaca agcgcataat acggggggca gcgaacaaga
     4201 agaagcgcaa gcgcataata cggggagcag cggaggggaa gcaacaccgg cagacagcac
     4261 agtagcatcc ggcccctttt tggttccgaa aaacatagac cttaacgtga atacgagcat
     4321 tatgaatgcc tatgtgggca atcaaatcgc caccaacctc accggaagac tttacgtcaa
     4381 ggacgatgct ctggtgctcg aagaaatcgg ttttgtcagc gccgccgcca gactcaagtt
     4441 gacggcctta taccgctcac cccgccccaa tcacctctac ctgggcctcg actatcatat
     4501 ggttgacatt cagattgacg aactgctaag catgtttccc gacctggaca ccattatgcc
     4561 catgctgagc tccttcaagg gccagggcga atttcatctg gctgccgaaa cttacctcga
     4621 taaaaactac aacctaaaaa aatccaccct gaggggagcc tcctccatac ggggcgacaa
     4681 cctggtgttg atggacagcg aaacctttgg aaaaatctcc aaaattctga tgttcaacaa
     4741 aaagaccgaa aacaaaatag acagcctgag cgccgaattc acggtattca aaaacgaagt
     4801 ggatgtgtat ccgtttacca tagtgatgga taaatacaaa gctgtcgtgg gaggccgcca
     4861 caacctcgac atgagtttcg attatcatat ttctttgctc gaaagcccgc tgcccatgcg
     4921 cctgggtgta aacatcagcg gcactatgga cgaccttaag atcaggccgg ccgcctgtaa
     4981 ataccccgaa aattaccgcc cggtaagccg gaggatagtc gatgcccaaa aattggaatt
     5041 acgcaaactg attcgggaaa ccctgatcaa gcaaatggaa taaaaacagg gcgtctttgt
     5101 gggacgccct gtgtacatag atttgccgcc tgctaaaaca gattcgggaa aaagacggaa
     5161 aagaccaaaa taaacaagcc cagaatcaaa acgacaatgg ttttggttga tactcctttc
     5221 cattccttga gcaagattcc ccagacattg ctgaaggtaa cgttcaaggc catcagtata
     5281 ctccacgaga aagccagcat aatggcattc tctgccagga aacttttacc gacggataaa
     5341 ccgaaaaatt gagaatacca gagtaatcct gccaaagagc agaaaaacag gttattaacc
     5401 catattttac ggctatttga agtgtagtct ttgaagctct tatttttggc gttttgcgac
     5461 aaacaataaa cggcattggt gaaaaaaccg cctaaagtta ccataagagt ggtcgggaga
     5521 gtggcaaaca aatcattggc accaacactt ttggctgcgt ctgcaatggg ttgtcctgcg
     5581 ctgagtccca gagcaaaaca agcactcatt acaccggcca gcaatgccac caacaaacct
     5641 ttggtcaaag caaaatcctt aatagctgct ttcttttgct cttcggtcat gttttgagct
     5701 ctcaggccac cggcatagcc gattacggcg ataccgacca gtgttataca aacacccagt
     5761 aaaagaatca ggccgtcccc gtgaagcaaa tcctgtttat caaaaaacaa agccggaatc
     5821 aaggttccaa aagcagagca agtacctaat gctatggatt gtcccagggc aacgcccagg
     5881 taacgcatac tgagtccgaa agtcaggccg cccacgcccc aaagggcgcc aaagcctatg
     5941 gctttccacg aagcagcggg atcaacactc aacaaagcgc ccaatgtcga cccctgaggt
     6001 actgccagca gggctcccaa aaagggaaaa atcagccagg caaacacccc ctgaaccagc
     6061 cagaaacttt cccacgacca attcttaatc ttgttgatgg gtacataaga actggactgc
     6121 ccgaaactgc caacggcaat caccagcaaa cctatcagcg tattcatggt cagcgtttag
     6181 aagttac
//
LOCUS       DAOSGL010000102         6165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11208_length_6165_cov_4.325859, whole genome shotgun sequence.
ACCESSION   DAOSGL010000102 DAOSGL010000000
VERSION     DAOSGL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11208_length_6165_cov_4.325859"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKY68_04410"
     CDS             <1..245
                     /locus_tag="PKY68_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005652052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOH24313.1"
                     /translation="YWFAIVFRKVTAKPKKTGILRSPVRADEIPGEVIAAISAALYEL
                     DNDVHDIEHTIITIERTRRTYSPWSSKIYGLRRLPQ"
     gene            270..698
                     /locus_tag="PKY68_04415"
     CDS             270..698
                     /locus_tag="PKY68_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOH24314.1"
                     /translation="MKEFKYNIQGFQYKVEIINEQKNLIELEVNGTPYTVQIERPEKS
                     KLITTKPAAAPVTPEGTPVVAQAKTKSPGGAVKSPLPGVILSVNVKVGDQVKIGSKLL
                     VLEAMKMENTISSDRDGKVLEIKVEKGASVLEGAELVIIG"
     gene            700..1857
                     /locus_tag="PKY68_04420"
     CDS             700..1857
                     /locus_tag="PKY68_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOH24315.1"
                     /translation="MGDFLQFLGENIKEFLTYTGFANATSGHLIMILVGLVFIYLAVR
                     HEFEPMLLIPIGFGMLIGNIPFKEVGLELGIYEEGSVLNILHQGVLKGWYPPLVFLGI
                     GAMTDFSSLISNPKLMLVGAAAQIGIFASYSLALSLGFDPKQAAAIGIIGGADGPTAI
                     FLSSKLAPNFLGAIAVSAYSYMALVPVIQKPVMRLLTTKKERVIRMKPPRIVSSTEKI
                     VFPIAGLLMTAFMVPSGLPLLGMLFLGNLLKESGVTRRLAETARGPLIDTITILLGLT
                     VGASTQASQFLTWDSIKIFTLGAFSFIIATAGGVLFVKLFNLFLSKDSKINPLIGNAG
                     VSAVPASARISQVMGLKYDPKNYLLMHAMGPNVAGVIGSAVAAGVLLGFLG"
     gene            2166..3086
                     /locus_tag="PKY68_04425"
     CDS             2166..3086
                     /locus_tag="PKY68_04425"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOH24316.1"
                     /translation="MGNVHIQGIIPPMITPLEDMNSLDVPGLEKLVEHILSGGVHGLF
                     ILGTTGEAQSLSYELRHELIERVCRQVGKRVPVLVGISDTSLVESMHLAEKAFAEGAT
                     AVVSAPPYYYAPAQQELIDFYQSLADVLPLPLFLYNMPSHVKVKIEPATLKAIAQHPN
                     VIGLKDSSSDMVYFHSLIQIMKDKEGFSLLVGPEELTAEAVLLGADGGVNGGANMFPS
                     LYVNMYKAAKSRDLDTVLALQQQIMYISNSIYKIGKYGSSYLKGVKCTCSLLGLCSDY
                     MALPFQRFNAPERKRVGQILENLGYTVINP"
     gene            3122..4636
                     /locus_tag="PKY68_04430"
     CDS             3122..4636
                     /locus_tag="PKY68_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOH24317.1"
                     /translation="MTILDYIVFIAFLVGIVLFGSSFYFRNKKKQSTDHFTVAGGNLP
                     SWVVGMSIFATFVSSISFLGYPGEAYTNNWNPFVFSLSIPIATWLAAKLFVPLYRSMN
                     SPSAYHYLEVRFGYWARAYVAICYLLTQLARMGSILFLLALPLNFMLGWSVPVLILVT
                     GISVLIFSSLGGISAVVWTDAIQGIILIVGAVVCAILLTVSIPGGFSGLIEVGSQYGK
                     FSLGSLSCSLSEPTFWVLLIYGLFINLQNFGIDQNYIQRYMTARDNRSAQKSTLLGGL
                     LYVPVSLVFVYIGTALFVYYKTKGVMPEGISGDQVFPYYIVQALPTGISGLLIASILA
                     AGMSTVSTSLNSSSTVILTDFYQRIRKREAGEKEGMKILYTSSVVLGVLGMVIALLMM
                     RVDGVLTTWWKLASIFSGGMLGLFLLGFLSKTVKNPAAIAGVVAGLLVIAWMSLSPYL
                     FTEGALVKYKCNLHGNLTIVLGTTTIFLVGFLVSMALQAFSKKSKKPINKDLQE"
     gene            4667..5314
                     /locus_tag="PKY68_04435"
     CDS             4667..5314
                     /locus_tag="PKY68_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3826 domain-containing protein"
                     /protein_id="HOH24318.1"
                     /translation="MKKLILALVLFTGLSQAFAQQSDDDYRKVIYGRSETIAKSLDIQ
                     DKVKYDFILELIANQYIDLGVINDAFAAKENEIKASSLPDEEKTQAKDLAYLRQREAL
                     TVKHFYFVNQLNANLSPEQVEKVKDGMTMGVYPVTYKAHLEMIPGLTEEEKTYIRAAL
                     MEAREYAMDCSDSKAKHAWFGKYKGRINNYLSKRGYNLTKEREAWNERIKASQAK"
     gene            5341..>6165
                     /locus_tag="PKY68_04440"
     CDS             5341..>6165
                     /locus_tag="PKY68_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOH24319.1"
                     /translation="MKKTVFAFIIGLSSVAVFPQYSARMAQPAVQANQSGLLEYTRDA
                     RANHIPDYSYCGYMASEQLIPEVPVKAVVSWMPGDNTARIQQAIDYVSSLEPDAQGFR
                     GAVLLEKGCYEIAGGLTISASGVVLRGSGYMDGQTELLGTGMDRTTMIRVAGKKDYQA
                     GQNKEISSYVPTGARAVVVQGHSFEPGDRIRITRPGTQAWIDAMDMNDFGGESSYIGW
                     KPEDMFVYWERQVKEVRGDSILLDAPLSCSLDPEFGGGFVSSFEWPGRISRSGVENL"
BASE COUNT         1615 a         1258 c         1477 g         1815 t
ORIGIN      
        1 tttactggtt tgccatagtt ttccgtaagg tcacagcgaa acctaaaaaa acaggtatac
       61 ttcggagtcc tgtgagagct gacgaaattc cgggtgaggt tattgctgcg atttctgctg
      121 ctctgtatga attagataac gatgttcacg atattgaaca tacaatcatc accattgaac
      181 gtacaagaag aacttattct ccgtggagtt caaagattta tggcttgcgc agactgccac
      241 aataatattt actataaaat caagtataaa tgaaagagtt taaatacaac attcagggtt
      301 ttcagtataa agttgaaatt atcaatgaac aaaaaaattt gattgagctt gaagttaacg
      361 ggacacctta tacagttcaa atagagcgtc cggaaaaaag caaactgatc actactaaac
      421 ctgctgcggc tcctgttact cccgagggga caccggtagt ggctcaggca aaaacaaaaa
      481 gtcccggagg ggccgtaaaa tctcctttac ccggagtaat tttgagtgtt aatgtcaaag
      541 tgggcgatca ggttaaaatt ggctcaaaat tattggtgct ggaagcaatg aagatggaaa
      601 atactatttc tagcgaccga gacggaaaag tgttggaaat aaaagtagaa aaaggggcct
      661 ctgtgctcga aggtgctgaa ctggtaatta taggttgata tgggagattt tttgcaattt
      721 ttaggcgaga atattaaaga atttctcaca tataccggtt tcgccaatgc gacttcgggg
      781 catttgatta tgatactggt gggcttggta ttcatttatc ttgccgtacg tcacgaattt
      841 gagcccatgt tgttgatacc tataggtttt gggatgttaa tcggtaatat tcccttcaaa
      901 gaagtgggtc tcgagttggg tatttacgag gaaggctccg tgcttaatat tttgcatcaa
      961 ggcgttctca agggctggta tccgccattg gtcttccttg gtattggagc catgacagac
     1021 ttttcgtcgc ttatttccaa tcccaaactg atgctcgtag gggcagctgc tcaaatcggt
     1081 atatttgctt cttactcttt ggcactcagt ttgggatttg accccaaaca agctgcagca
     1141 atcggtatta ttgggggtgc tgatggtcct acggcaatat ttctttcgtc aaaactggca
     1201 cccaactttt tgggagctat agccgtttcg gcctattcat atatggcctt ggttcctgtg
     1261 attcaaaaac cggttatgcg attgctgacc acaaagaagg aaagggtcat ccggatgaaa
     1321 cctccacgta tagtttcttc taccgaaaaa attgttttcc cgattgccgg tttgttgatg
     1381 actgcattta tggttccttc gggtttgccc ttgctgggta tgctcttttt aggaaattta
     1441 ctcaaagaaa gcggtgtaac aagacgtttg gccgagaccg caagaggtcc gctgattgat
     1501 actatcacca tattattggg tttgacggta ggagcttcta ctcaggcttc gcaattcctt
     1561 acttgggatt caataaaaat attcactctt ggagctttct cctttattat tgctacagcc
     1621 ggcggtgttc tctttgtgaa attattcaat ttgtttcttt ccaaagacag caaaatcaat
     1681 cctttaatag gaaatgcagg agtgtctgcc gtccccgctt ctgcaagaat ttcccaagtg
     1741 atgggattaa aatacgatcc caaaaattat ttattgatgc acgccatggg tcccaatgtg
     1801 gcaggtgtta tcgggtcggc agtggctgcg ggtgttttgc tcggattttt gggctaatgt
     1861 ttattccttt ggggaaaata ccataaccag tattctcgag agagtgttct tacaaaaagg
     1921 cactctcttt tttttgagta ttacccgaca tcttactcgt tacatcggat taggttgcct
     1981 gttaatataa attgttcata attattattt gtttaaaacg atttagattt atatttgtaa
     2041 gattttcttt aaaaatacga cggaggtcaa accggaaaga cggcagctct cccaaccgta
     2101 ttcgtatatt gagaataggg caggagacca ttaagcattt aattcaataa taataaaata
     2161 gaactatggg aaatgtgcat attcaaggta ttatacctcc catgattact cctctcgaag
     2221 atatgaacag cctcgatgtt ccgggtttgg aaaaattggt cgaacatatt ttatcgggcg
     2281 gggttcatgg tttgtttatt ttaggaacta ccggcgaggc tcaaagcctg agttatgaac
     2341 ttcgtcacga gttgatagaa cgggtttgcc gtcaggtggg aaaacgagtt ccggtgttgg
     2401 tgggtatttc cgatacttct ttggtggaaa gtatgcattt ggccgaaaaa gcttttgccg
     2461 aaggagccac ggcagtggtg tcggctccgc cttattatta cgctccggct cagcaggaat
     2521 tgatcgattt ttatcagagc cttgccgatg ttttgcctct gcctttgttt ttgtacaata
     2581 tgccttcgca tgtcaaggta aaaatagaac ccgccacgct caaagctata gcacaacacc
     2641 ccaatgtgat cggccttaag gatagttctt ccgatatggt gtatttccat tccctgatac
     2701 agatcatgaa agacaaagag ggtttttccc ttttggtagg ccccgaagaa ttgacggccg
     2761 aagcggtact tttgggagcc gacggcggag tgaacggtgg ggccaatatg ttcccctcgc
     2821 tttatgtaaa catgtataag gcagcaaaat cacgcgattt ggataccgtt ctggccttgc
     2881 aacagcaaat aatgtacatt agcaacagta tatacaaaat cggcaagtac ggttccagtt
     2941 atttaaaggg agtcaagtgt acctgttccc tgctgggcct ttgcagcgat tacatggctc
     3001 tgccttttca acgcttcaat gcacctgaaa gaaaacgggt agggcaaatt ttagaaaacc
     3061 tgggctatac agtaatcaat ccctgaaatc aatccaataa gctatattca tgactaccga
     3121 tattaccata ctcgattaca ttgtttttat tgccttcctg gtgggcatag ttttatttgg
     3181 ctcttctttt tatttccgga ataaaaagaa gcaatctacc gaccatttta cggttgcggg
     3241 cggcaatttg ccgtcctggg tggttggaat gtccatcttt gctacctttg taagcagtat
     3301 cagtttcctg ggctatcccg gcgaagccta taccaacaac tggaatccct ttgttttcag
     3361 cttgtccatt cccatcgcca cctggttggc tgccaaactg tttgtgccgc tttatcgaag
     3421 tatgaacagc ccttcggctt atcactactt agaggtaaga ttcggctatt gggccagggc
     3481 ctacgtggct atctgttatt tattgacgca attggccagg atggggtcta ttttgttttt
     3541 gttggctctg ccgcttaatt ttatgctggg ctggagtgtc cccgtcctta tattggtaac
     3601 cggtatttcg gtattgattt tttcgtcatt gggtggtatt tcggcggttg tttggaccga
     3661 tgctattcag ggtataattt taatcgtggg agctgtggtc tgtgctatct tgctaacagt
     3721 aagtattccc ggaggttttt cgggcttgat agaggtggga tcgcaatacg gaaaattcag
     3781 cttgggcagc ctgagctgca gtctgagtga gcctaccttt tgggttctgt tgatttacgg
     3841 cttgtttatt aacctgcaaa attttgggat agaccaaaat tacatccaac gttatatgac
     3901 agccagggat aatcgcagtg cccaaaaatc cactttgctt ggtggcttgc tctatgttcc
     3961 tgtttccctg gtgtttgtgt acatcggaac agccttgttt gtttattata aaaccaaagg
     4021 tgtgatgccc gaaggaatca gcggagatca ggtttttcct tattacattg ttcaggctct
     4081 gcccacggga attagcggtc tgcttattgc cagtattctg gcagcaggta tgagcactgt
     4141 ttccaccagt ctgaacagtt cctctacagt gatactgact gacttttacc aaagaattcg
     4201 taagcgggaa gccggtgaaa aagaggggat gaagatactc tacacatcaa gtgttgtttt
     4261 aggcgtatta ggtatggtaa tagctttgtt gatgatgaga gtcgacggag tgttaacaac
     4321 ctggtggaaa ctggcttcca tttttagcgg aggaatgctg ggcttatttt tactgggctt
     4381 tttgagcaaa acggtcaaaa acccggcagc tatagccggt gtggtggccg gtttgctggt
     4441 gattgcctgg atgagcctga gcccttacct ttttaccgaa ggggccttgg taaagtataa
     4501 atgcaatttg cacggcaacc tgaccatagt tttgggaacc acgactatat ttttggtcgg
     4561 atttttggtg tcgatggcct tgcaggcttt ctctaaaaag tctaaaaagc ctatcaataa
     4621 agacttacaa gaataaatca tttaaacaga gactgtcgaa atccttatga aaaaattgat
     4681 tctcgcctta gtgcttttca ccggtctgag ccaggctttc gctcaacaat ccgacgacga
     4741 ttaccgcaaa gtaatttacg gccgttcgga aaccatagcg aaaagcctcg atattcaaga
     4801 caaagttaaa tacgacttta tactggaatt aatagccaat caatacatcg atttgggtgt
     4861 catcaacgat gcctttgctg ccaaagaaaa cgaaatcaag gcttcttcgt tgccggacga
     4921 agaaaagacc caggccaaag acctggctta tcttaggcaa cgtgaggctc ttacggtcaa
     4981 gcatttctat tttgtcaatc aattgaatgc caatttatcg cccgagcaag tagaaaaagt
     5041 gaaagacgga atgaccatgg gcgtatatcc tgttacatac aaagcacatt tggaaatgat
     5101 acccggcctg acagaggaag aaaagaccta tatcagggct gccctgatgg aagcccgcga
     5161 atacgccatg gattgttccg actccaaagc caagcatgcc tggtttggta aatacaaagg
     5221 ccgtataaac aactatcttt ccaaaagagg ttacaatctg actaaggaac gtgaagcctg
     5281 gaacgagcgt attaaggcct cccaggcaaa ataattaata tcatgcccga ttcttttatc
     5341 atgaaaaaaa cagtatttgc atttataatc ggccttagta gcgtggctgt ttttccgcaa
     5401 tattcagcga gaatggctca gccggctgtt caagccaatc agtccggctt gcttgaatat
     5461 acccgggatg cccgtgccaa tcatataccg gactattctt attgcggcta catggcttcc
     5521 gagcaactta ttcccgaagt gcccgttaag gccgtggttt cttggatgcc gggagataat
     5581 acggctcgaa ttcagcaggc catcgattat gtttcctcgc tggagcccga tgctcagggt
     5641 ttcagaggag ctgtcttgct ggaaaaggga tgttatgaaa tcgccggggg gctgaccata
     5701 tcggcttcgg gggttgtttt acgtggcagt ggatatatgg acggccaaac ggaacttttg
     5761 ggtaccggga tggaccggac caccatgatt cgtgttgccg ggaaaaaaga ctatcaagca
     5821 ggccaaaaca aggagataag ctcttatgtc cccaccggag caagggccgt tgtggttcaa
     5881 ggtcattcct tcgagcccgg agaccgcata cgtattacac gcccgggtac tcaggcatgg
     5941 atagacgcta tggacatgaa cgattttggc ggggagtctt cctacatagg ctggaaaccc
     6001 gaagatatgt ttgtctattg ggaacggcag gttaaagaag tgcggggcga cagtattctg
     6061 ctcgatgctc ctttgagttg ttctttggat cccgaatttg gcggaggttt tgtgagcagt
     6121 tttgaatggc ccggacgtat aagccgctcg ggcgtggaga acctc
//
LOCUS       DAOSGL010000103         6125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11303_length_6125_cov_4.008237, whole genome shotgun sequence.
ACCESSION   DAOSGL010000103 DAOSGL010000000
VERSION     DAOSGL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11303_length_6125_cov_4.008237"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKY68_04445"
     CDS             <1..237
                     /locus_tag="PKY68_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOH24320.1"
                     /translation="ERVTEGPNMPLIRHMVLSNIHSNKSKYGVWIDGYTDRINVHDVL
                     IRNCSFSNAEKGNLIVGAENVVFEDCLLNGEKIE"
     gene            complement(243..1214)
                     /locus_tag="PKY68_04450"
     CDS             complement(243..1214)
                     /locus_tag="PKY68_04450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOH24321.1"
                     /translation="MDAPNPISQAFDRQKLISVIVPVFNRARVLKFCLDSIRSQSYRP
                     LELILVDNGSEDQSLQICRDYQQQYHTADFNVVVLQETKKGACAARNKGYAASRGDYL
                     LFFDSDDRLYPDYAAVMLREAAVQNYPPLLACLADVEERGRRFVFPKRIQAQPAAQLY
                     DPVLKTASALIRRDLMKQSGPWDEDLQRWQDLEFGFRLLLNTPTVCWVLRPLFYSGVL
                     EDSITAASYTADHLKMNLSLEKIAQSIDRYDKNEEKPALLKALAFKTISMAALLYREQ
                     SFDLAKHYYKKGLSICPTKPGTILLRLHYLYTRLGGRGFWRLAEKIL"
     gene            1302..2339
                     /gene="murB"
                     /locus_tag="PKY68_04455"
     CDS             1302..2339
                     /gene="murB"
                     /locus_tag="PKY68_04455"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOH24322.1"
                     /translation="MKVQEHFCLKDKHSFHLNVTARYWAEYRNTDELGEMLQDKRFNK
                     LPVLPVGGGCNLLFTRDFPGLVIKSAISGLDIVHENEEEVVLRVGSGLDWDQFVESCV
                     SQGWGGIENLSGIPGLTGATPIQNIGAYGVEAKEVIRYVEVFDRQTRSLLVLPVEECG
                     LAYRNSRFKQEWKDRFIVCYITYRLNKKPAYRLDYGNLNEVLRSQGGLQEGPEAHRGN
                     LAMIRRAVLAIRSSKLPDPAVLGNAGSFFKNPELDVATYSALAERFPELPHWELPGNK
                     VKVSAAWMIEYCGWKGKSMGRAGVYPKQALVLVNLGDARGEEIYNLYQHIVADVHKAF
                     GISLQPEVVVL"
     gene            2451..3602
                     /gene="dnaN"
                     /locus_tag="PKY68_04460"
     CDS             2451..3602
                     /gene="dnaN"
                     /locus_tag="PKY68_04460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOH24323.1"
                     /translation="MKFIASSTTLLGHLQTISRVINPKNTLPALDNFLFKIEDGQLTV
                     TASDAENTLVTTMDLVESDSDGLFCIPAKNMLESLREIAEQPLHFEFTADSMELNIYY
                     RNGQFNFVAQDGMEYPQLRPLKNKLGEIVMPASLLYRGIASTLFAAADDELRPVMNGV
                     VMDIFPDNVTFVASDAHKLAKLTNASFHGEAPAEGEEVGASTLIIPRKPASILKNILP
                     KESEDVKIIFDENNAMFVMSDYTMLSRLLEGRFPNYNAVIPQNNPYTVLVDRLSFLNA
                     LKRVSVCADPANNLIKLDITNNNILITAQNIDFSTSAEENVECQYDSEPLSLGFKAVF
                     LIDIISNITTPEILLKLADPSRAGLILPSVNEESEELQMLLMPMMINRS"
     gene            3677..4444
                     /locus_tag="PKY68_04465"
     CDS             3677..4444
                     /locus_tag="PKY68_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH24324.1"
                     /translation="MKLNLKNPIVFFDLETTGINPATDRIVEISYLKIHPNGKEESRT
                     YRVNPGIPIPKEASEIHHITDEMVADCPGFKEVGQIIANDIKGCDLAGFNSNRFDVPL
                     LAEEFLRNDIDLDMSARKLIDVQVIFHKMEQRNLRAAYRFYCDKELEDAHSAEADTRA
                     TYEVLKAQLERYPELKNDVEFLAEFSTQNRNVDFAGKFIYNNKGEEVFNFGKYKDISV
                     EEVLKKDPGYYGWMMNGDFALNTKNVLTKIRLRSLQK"
     gene            4444..5643
                     /gene="coaBC"
                     /locus_tag="PKY68_04470"
     CDS             4444..5643
                     /gene="coaBC"
                     /locus_tag="PKY68_04470"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOH24325.1"
                     /translation="MMLKGKKIVLGITGSIAAYKSAYLVRLLVRKGAEVQVVMTPAAR
                     EFITPVTLSPLSGKAVISEFFTANSGLWHSHVDLGLWADLMLIAPATASTIGKMANGI
                     ADNMLITTYLSMKAPVFVAPAMDLDMYRHPSTTHNLELLKRYGNHIIEPASGELASGL
                     EGKGRMEEPEQIVAQLERFFSAQLDLKDKKVLITAGPTYEKIDPVRFIGNYSSGKMAY
                     ALAEACADRGARVELISGPVALQTRHPAIHRTDVETAAEMYEACMHYFPAVDVAILCA
                     AVADFSPENPRPSKIKSNKEGFGLSLKPTKDIALNLGNVKKPGQVLVGFALETDNEIE
                     NARAKMERKKLDFIVLNSLNDQGAGFRADTNKIKILEPGGKVLDFPLKSKQKVAEDIV
                     NHLITKL"
     gene            5640..>6125
                     /locus_tag="PKY68_04475"
     CDS             5640..>6125
                     /locus_tag="PKY68_04475"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOH24326.1"
                     /translation="MNSFKNIHISFVFLFLFFLSGRGLALAQELNCRVQVNSDQIGGT
                     DKAVYESLRNAVTAFMNERQWSNAPIAQNEKIDCNLLFIIQGSENNVHKAELQIQSRR
                     PVFGSSYTTSLLNIRQSLEFEYLENQQLEFNETNLQSNLTATLAFWTYIVLCMNFDSF
                     AP"
BASE COUNT         1679 a         1400 c         1490 g         1556 t
ORIGIN      
        1 gagcgagtaa ccgaagggcc taatatgccg ttaatccgtc acatggtttt gagcaacatt
       61 cactcaaaca aaagcaaata tggggtgtgg atagacggtt acaccgatcg tatcaacgtg
      121 cacgacgttc tgatacggaa ttgctccttc agcaacgccg aaaagggaaa cctgatcgtc
      181 ggagccgaga acgtggtttt tgaagactgc ctgctcaacg gggaaaagat agagtagcgg
      241 tcttaaagta ttttttcggc caagcgccag aatcctctgc cgccaaggcg ggtatacaaa
      301 taatgaagcc tgagtagtat tgttcccggt tttgtggggc aaatgctcag gccttttttg
      361 taataatgtt tggccaaatc gaaggattgc tcccggtaca gcaaggcagc catagaaata
      421 gtcttaaaag ccaatgcctt cagtaaagcc ggtttttctt cgtttttatc gtatctatcg
      481 atgctttgag cgattttctc gaggcttaga ttcatcttga ggtggtctgc cgtataagag
      541 gctgccgtga tggaatcttc caacacaccc gaataaaaga gcggccgcaa cacccagcag
      601 accgtcggtg tattgagcag caggcgaaaa ccgaattcca gatcctgcca gcgttgcagg
      661 tcttcgtccc aggggccgct ttgtttcatc aggtcgcgac gaataagggc ggaagcggtt
      721 ttcaggacgg gatcgtaaag ctgtgcggca ggctgtgcct gtatgcgttt gggaaaaaca
      781 aagcgccggc cacgttcttc gacatcggcc agacaggcca gcagcggggg ataattctgt
      841 acagctgctt ctctgagcat aacggcagcg tagtcgggat acaaacgatc gtcggaatcg
      901 aaaaagagca ggtaatcgcc cctcgaggcg gcatagcctt tgtttcgtgc ggcgcaggct
      961 cccttttttg tttcctgcaa caccacaacg ttgaaatctg ccgtatggta ttgctgctga
     1021 taatcgcggc agatttgcaa actttggtcc tccgaaccgt tatcgaccaa aatcaattcc
     1081 aagggacgat agctctggga acggatgcta tcgaggcaaa acttcaacac ccgggcccgg
     1141 ttgaaaacgg gaacaatcac agagatcagt ttctgtctgt cgaaggcctg agaaataggg
     1201 tttggggcat ccatgggtgc agattaagtc gggcgaaagt aggtaaaaat cccttaatta
     1261 tcaaaaatct ctcctatctt tgcgatccat tctttaagct aatgaaagta caagaacatt
     1321 tttgcttaaa agacaaacac agttttcatc tgaatgtgac tgcccgctat tgggcagagt
     1381 atcgtaacac cgatgagctc ggggaaatgc tgcaagataa acgtttcaat aagcttccgg
     1441 ttttgccggt gggcggaggc tgtaatttgc tttttacccg cgattttccc ggtctggtca
     1501 ttaaatcggc cattagcggt ttggatattg tgcatgaaaa cgaagaggaa gtcgtgctgc
     1561 gggtgggttc cggtctggat tgggatcaat ttgttgaaag ctgcgtttcg cagggctggg
     1621 gaggaattga aaatctttcg ggcataccgg gtctgacggg tgccaccccc attcaaaaca
     1681 tcggagctta cggcgtggaa gccaaagaag taatacgtta tgtcgaggtt tttgacaggc
     1741 aaactcgaag cctgctggtt ttacccgtcg aggaatgtgg attggcttac cgcaacagcc
     1801 gttttaaaca ggaatggaaa gaccgcttta tagtgtgtta tataacttac cgtctgaata
     1861 aaaagccggc ctatcgctta gattacggca atctgaatga agtactgcgt tcgcagggcg
     1921 gcctgcagga ggggcctgag gcacatcgag gcaacctggc tatgattcgt cgtgcggtgt
     1981 tggccattcg ttcctcaaaa cttccggatc ctgccgtttt gggcaatgcc ggcagttttt
     2041 tcaagaatcc cgagttggat gtcgcgactt attctgcttt ggctgaacgc tttcctgagc
     2101 tgccgcattg ggagcttccc ggcaacaaag ttaaagtgtc ggcagcctgg atgattgaat
     2161 attgcggatg gaaaggcaag tcgatgggca gggcgggagt ttatcccaaa caagctctgg
     2221 ttctggtaaa tctgggcgat gcccggggag aggagatcta caatttatac caacatattg
     2281 ttgccgatgt gcataaagcg ttcggtatca gccttcaacc cgaagtggtt gtgttatgag
     2341 attaagtatt gataatgtgt agtttaatta gttttttatt tttttcggcc tgagctcagg
     2401 gctattctat ttttttcata actttgtagc tcatctaaac aatttccacc atgaaattta
     2461 ttgcatcaag tactacatta ttgggtcatc ttcaaaccat cagcagagtc atcaatccca
     2521 aaaacacctt gcctgcgctg gataatttcc tgtttaaaat cgaggatggc cagttgacgg
     2581 ttaccgcctc ggacgcggaa aatacgctgg taactactat ggacttagtt gaatccgatt
     2641 ccgacggcct gttttgcatt ccggccaaaa acatgctcga atcgcttaga gagattgccg
     2701 aacaaccttt gcattttgaa tttacggccg acagcatgga gttgaatatc tactacagaa
     2761 acgggcaatt caattttgtg gctcaggacg gcatggaata tcctcagttg agaccgctca
     2821 aaaacaagct cggagaaatt gtaatgccgg cttctctttt gtacaggggt attgcttcta
     2881 ctctttttgc agcggccgat gacgagttga ggccggtgat gaatggggtg gtgatggata
     2941 tttttcccga taatgtcact tttgtggctt cggatgctca taaactggcc aaactgacca
     3001 acgcttcctt ccacggagaa gcgcctgccg aaggcgaaga agttggggct tccacgttga
     3061 tcattcccag aaaaccggct tcgatcttaa aaaacatact gcccaaggaa agcgaagacg
     3121 tcaaaattat ctttgacgaa aacaatgcta tgtttgtgat gtcggattac accatgttaa
     3181 gccgtctgct cgagggacgt tttcccaatt acaatgcggt tattcctcag aacaatcctt
     3241 atacggtgct ggtcgatcgc ctctctttcc tgaatgccct taagcgggtg tcggtttgtg
     3301 ccgatccggc caacaacctg attaaactgg atataacaaa caacaacata cttatcacag
     3361 cccaaaacat tgatttttcc acttctgccg aagaaaacgt ggaatgccag tacgacagcg
     3421 aaccgctgag cttgggattt aaggctgtat ttttgattga cattatatcc aatattacta
     3481 cgcccgaaat ccttctgaag ctggcagatc cttccagagc cggactgata ctaccgtccg
     3541 taaacgaaga aagcgaagaa ctacagatgc tgctgatgcc tatgatgatc aacagaagct
     3601 aggcgggaac accggcaaat taagcttttc aaccctttgg cagccggagg aaatgaatcg
     3661 ctacaaactg taaaacatga aactgaactt aaaaaatccc atcgtttttt tcgatttgga
     3721 aacgaccggt attaatcctg ctaccgacag gatagtcgag atctcctatt tgaaaataca
     3781 tcccaatggg aaagaagaat cgcgtacata cagggtaaac cccggaattc ctatacccaa
     3841 agaagccagc gagattcatc acatcaccga tgaaatggtg gccgactgtc ccggttttaa
     3901 agaagtcgga cagataattg ccaacgacat caagggttgt gatttggccg gatttaattc
     3961 caatcgtttc gatgttcccc ttttggccga agaatttttg cgcaacgata tagacctgga
     4021 catgagtgcc cgcaaactta tcgatgtaca ggtgattttt cacaaaatgg aacaacgcaa
     4081 cctgagagcc gcctatcgct tttactgcga caaggagctg gaagatgccc acagtgccga
     4141 agccgacaca agggcaacct acgaagtgct caaggctcag ttggaacgtt accccgaatt
     4201 gaaaaacgac gtggaatttc tggccgaatt ttctacccag aatcgcaacg tagattttgc
     4261 cggtaaattt atttacaaca ataagggcga agaagtcttc aattttggca aatacaaaga
     4321 tatttccgtc gaagaagtat tgaaaaagga ccccggctat tacggctgga tgatgaacgg
     4381 agattttgcc cttaacacca agaatgtgct taccaaaatc agattacgtt ctttgcagaa
     4441 ataatcatgt tgaaaggaaa aaaaatcgta ttgggcatta ccgggagcat agccgcttac
     4501 aaatcggcct atttagtgag gcttctcgta cgtaaaggag ccgaagtgca agtagtaatg
     4561 actcccgccg ccagagaatt tatcacaccg gtcactttga gccctttatc gggcaaagcg
     4621 gtgatcagcg aatttttcac agccaacagc ggactgtggc acagtcacgt cgatctggga
     4681 ttgtgggccg atcttatgct gatagctccg gctacggctt cgaccatagg caagatggcc
     4741 aacggcattg cggacaatat gctgataacg acttatcttt cgatgaaagc tccggtgttt
     4801 gtggctccgg ctatggacct ggatatgtac cggcatcctt ccaccacaca caatttggaa
     4861 ttgctcaaac gctacggcaa tcacatcata gagcctgcca gcggagaact ggccagcggt
     4921 ctcgaaggca aaggccgcat ggaagaacct gaacaaattg tcgctcaact cgaacgtttt
     4981 ttttccgctc aacttgattt gaaagacaaa aaagttttga ttaccgcagg tcccacctac
     5041 gaaaaaattg accctgtacg ttttataggc aactattcct ccggtaaaat ggcctacgca
     5101 ctggccgaag cctgcgccga cagaggagct cgggtggagt tgatttccgg gccggtagcg
     5161 ctccaaaccc gccatcctgc catacaccgt accgacgtag aaactgccgc tgagatgtac
     5221 gaggcctgta tgcactattt ccccgccgtc gatgtcgcga ttttatgtgc cgcggtggcc
     5281 gacttcagtc ccgaaaatcc ccggccttct aaaatcaaga gcaacaaaga aggcttcggc
     5341 ctctcgttga aaccgaccaa agacattgct ttaaacctgg ggaacgtgaa aaaacccgga
     5401 caagtgttgg ttggctttgc tttggaaaca gacaacgaaa ttgaaaatgc ccgagccaaa
     5461 atggagcgga agaaacttga tttcatcgta ttgaattctc tcaacgacca aggggccggt
     5521 ttcagagccg ataccaataa aatcaagatt ttggaacccg gaggaaaagt gttggatttc
     5581 ccgctcaaaa gcaaacagaa ggtggccgaa gacattgtaa accatctaat tactaagctg
     5641 tgaattcttt taaaaacata catataagtt ttgtttttct ttttctgttt tttttatcgg
     5701 gcaggggatt ggctttggct caggaattga actgccgggt gcaggttaat tccgatcaga
     5761 tagggggtac cgacaaggct gtctacgaaa gcctgcgaaa cgcagttacc gcctttatga
     5821 acgaaaggca atggtcgaac gctcctatag cccaaaacga aaaaatcgat tgcaaccttt
     5881 tgttcatcat acaaggcagt gagaataacg tacataaggc ggagcttcag atacaatcga
     5941 gaaggccggt tttcggctct tcttacacaa ccagcctgct caatatccgg caaagtttgg
     6001 aatttgaata cctggaaaat caacaactgg aattcaacga aaccaatctg caaagcaatt
     6061 taaccgccac cctggctttt tggacctaca tcgttctttg catgaatttt gacagtttcg
     6121 ctccc
//
LOCUS       DAOSGL010000104         6121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11316_length_6121_cov_3.707221, whole genome shotgun sequence.
ACCESSION   DAOSGL010000104 DAOSGL010000000
VERSION     DAOSGL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11316_length_6121_cov_3.707221"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKY68_04480"
     CDS             <1..1003
                     /locus_tag="PKY68_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HOH24327.1"
                     /translation="NSDSERLYMVKAVRYVKDAWINKGGGLMDFEEDLRQFGADIFIV
                     NEDGHSPAKAALCRELGIEYKVLQRIPESGLPARSTTALRSGNHCNLPYRLDLAGTWI
                     DQPYVSKFAPGWALTISLEPTVDFLERCGMSTSTRNAARRIWPYELPNEHPEKLAEIL
                     FRYENEPGRREISGAQDSIGICMPGLNRHYYDNNYWPEKIESCHDEAILNWLEEHIYM
                     VLLWPRKQGLNVLEGSRIDKEGVEALAAAADVCWNAIMQRDLKTFASSYLASFHAQTA
                     MFPAMMAPGVAEVIDHYRDQALAWKMAGAGGGGYLALVCEKPLPNAIRIKIRRKGWS"
     gene            1252..2028
                     /locus_tag="PKY68_04485"
     CDS             1252..2028
                     /locus_tag="PKY68_04485"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOH24328.1"
                     /translation="MDLRQYVPGIKKGDQLKIDLQHALVSYSYTAGVEDDGSLMLPGF
                     EKLQVLGMTTRALEDLLAVKSRTVHFSSAWLKTVHESPGGAGAASSESAVEVSSESTS
                     GVSSESLGLASSGRSGADPAVVSSLFSPKVTIANYRISVLGLVERPGQYPVPQQQITV
                     FEALALAGYKTINTKHGTIKVLREAADGQLIFYTLHLNDKSIVNSPAYYLEQNDVLYV
                     HPTRTGKWASGLSSPLALGVASLVSLLISAATLVVHIVKP"
     gene            complement(2164..3873)
                     /locus_tag="PKY68_04490"
                     /pseudo
     CDS             complement(2164..3873)
                     /locus_tag="PKY68_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase"
     gene            complement(4150..5100)
                     /locus_tag="PKY68_04495"
     CDS             complement(4150..5100)
                     /locus_tag="PKY68_04495"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HOH24329.1"
                     /translation="MYDCAARLVTWDIDKIRDYGKNYIGRQSVNMTRDSLNYWRINPE
                     RCLENYGYNVIRLNVPQAGKEVKAHFVGLAGADGYRKLNVDKAGWRYGFVALKENNGR
                     VYSDMFNQADDTAHFAIPDSCSMLWMVVTGAPTTHWRHAWDDDDSNDEHWPYKVKFEQ
                     TNLYGQYDFSGDYQRYDTTLYYEVELPYSSNSYLSTPLSINIDPVCMALGVTGTQLRT
                     ELGNKLRFVGFNANGTIAGNYTANGYGHWFSAEGNVINWGAGSYVYSEYQENSYTFNI
                     GQYPGRCPKGSKYTIRQGFRYQAPDNKTYQVKLVFTITMI"
     gene            complement(5057..6079)
                     /locus_tag="PKY68_04500"
     CDS             complement(5057..6079)
                     /locus_tag="PKY68_04500"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HOH24330.1"
                     /translation="MKKNLLLILLLGFGLQSPAQSELYVVKQDRSYESIDLNNIDSIV
                     WRSSIYRLYKNGSYKTNLYSNFQYLSFEKPPKQIFTQADDLRNLNLNNSSSKYCWERS
                     RESENFICFWEAGLGSDPINAGSKRVDVDDLLAKAEIYYDVYKNRMKFIIPGESRADK
                     YKMIIYLWDSDEWGAYGGGSSDVIGAIWVSTHTCKPVGATIAHEIGHAFQYQTYADYR
                     NGCGWRYGFGPNGSGGNGFWEQCANWQAYDIYPNEKFTNYNFGVYLDNYHKHMLHETP
                     RYANYFIQDYWVMKHGEDFIGKLWRGARYPEDPVEAYQSYRHRSDRFQRRNVRLCRPS
                     GHLGHR"
BASE COUNT         1336 a         1428 c         1705 g         1652 t
ORIGIN      
        1 caattccgac tcggagcgcc tttatatggt aaaggccgtg cgttatgtaa aagatgcctg
       61 gataaacaag ggcggcgggc tgatggattt tgaagaagac ctgaggcaat tcggggccga
      121 tatttttatt gtgaacgaag acgggcattc gccggcgaag gcggctttgt gtcgggagct
      181 gggcatagaa tataaagtgt tgcaacgcat acccgaatcg ggtttgcctg ctcgttctac
      241 cactgcactg cgcagcggga atcattgcaa tctgccctac aggctcgatt tggccggtac
      301 ctggatagat caaccctacg tttcgaaatt tgctccgggt tgggccttga ctatttcgct
      361 ggaacctacg gtagattttt tggaacgttg cggcatgtct acttctaccc gcaacgcggc
      421 gcgccgtata tggccttacg aattgcccaa cgagcatccc gagaagctgg ccgaaatatt
      481 gttccgttac gaaaacgagc ccggacgccg ggaaatttcg ggagcgcagg attccatcgg
      541 tatctgtatg cccggcctga accggcatta ttacgacaac aattattggc ccgaaaagat
      601 agaatcctgc cacgatgagg caattttaaa ctggcttgag gagcatattt atatggtctt
      661 gctttggccg cgcaagcaag gtctgaatgt gttggaaggc tcccggatcg acaaggaagg
      721 cgtcgaagcg ctggccgccg ccgccgatgt ttgttggaat gccattatgc agcgtgattt
      781 gaagactttt gcttcctctt atctggcatc gttccatgcc cagacagcca tgttcccggc
      841 catgatggct ccgggtgtgg cagaagtcat cgatcattac cgcgaccagg ccttggcctg
      901 gaagatggcg ggagccggcg gtgggggtta cctggctttg gtttgcgaaa aacctctgcc
      961 caacgctatt cgcattaaga tacgacgcaa ggggtggtcg taattaattg tttgagaata
     1021 agacacggta tttgtttaag aatagaacgt catagctgtt cttggtccgt ttattcagaa
     1081 gcttgttgta tgattattaa aaagaataga ccgaactcgt tgtttgccgg aaaggaaccg
     1141 cttacaaaca gtttactatc gtcgttttta ttgttgaccg cttgtttatt gttgactgct
     1201 tgcggcagca gcagagagcc ggcgtcgttt tccgggcaaa cgcagcaagc tgtggatttg
     1261 cgacaatacg tccccggaat aaaaaaggga gatcaattga agatcgacct gcaacacgca
     1321 ctggtatcgt acagttatac agccggggta gaggacgacg gcagtcttat gttgcccggc
     1381 ttcgaaaagc tgcaggtgtt gggcatgacc acccgggctc tagaagactt gctggcggtg
     1441 aaatcccgga cggtgcattt ttcttcggcc tggctcaaaa cggtgcacga atctccggga
     1501 ggcgcaggtg cggcttcttc ggaaagcgcg gtcgaggttt cttcggaaag cacgagcgga
     1561 gtttcttcgg aaagtctggg tttggcttct tcgggaagat ccggggcgga cccggccgtc
     1621 gtttcaagcc tgttcagccc caaagtgacg atcgccaatt accgtataag tgtgttgggc
     1681 ctggtggaac gccccgggca atacccggtg cctcaacagc agattaccgt tttcgaagct
     1741 ttggctctgg ccggttataa gaccataaat accaaacacg gcactatcaa ggtgttgcgc
     1801 gaagccgccg acgggcaatt gattttttac accctgcatc tgaacgacaa aagtattgtc
     1861 aattctcccg cctattacct ggagcagaac gacgtactct atgtgcatcc cacacgcacg
     1921 ggcaaatggg cctcaggtct gagttcaccg cttgcgttgg gagtagcttc gctggtttct
     1981 cttttgattt cggcggccac tctggtggtg catattgtga aaccctgatt acttattctg
     2041 ttccgggctt ttgtggcagc tgtaactgta accgcgggct tttttacgcc ggaaaagtag
     2101 cagggcaagc atgattgcaa ttagtacaat aaccacaata ttgctgttgt tgcttgcccg
     2161 gtccatcaga ctgcttttgc cgtcgagggt gtcgcgattg ttgccgggct tggtccaatc
     2221 gatttttttg cgggtatcta tgccgttcag gtatttctcg atgttggtgt agccgtcgcc
     2281 gttgcaatcc aatattgcgt ccgaaggatc gttgggattg agtttattgg ctttttccca
     2341 ggcgtcgggc attccgtcca tatcgctgtc aaggtaaggt tctcccttgt attcgggtaa
     2401 tccgcctact tgctggatat gggtgatgat accgcttttg taggagtcgg cgggcagccg
     2461 gcgcttgacg tagggtgttt cgaattcttc ggcatcggga gctaaaatag cctggccggt
     2521 aatcactgtt ttgatgatac gttcgtccac ggcatcgcgt ttgggcaaag tggctcccac
     2581 tttttccata acaaacctaa aggccgcttc ggcagtcatg atgtcctttt cggaaaaatt
     2641 gggcagggga gcgggttctt ttgccctcag catggcggtg taagggccgg tagctgaact
     2701 atcactcagc tgaacgcctc cttcccagtt gtcgcgactg acaatggggt tgccgtcgac
     2761 gtaattacct tccacataga tatgtccacg tctgggagtg cgatcgggtt ggtcgggagc
     2821 cggaacttgt tcgtcctgac taatattcat catacgtttt aggttgtcgg tgcttgtctc
     2881 gagtttgacc acccggtgtt cgatggcttt tcctttggga gtgatggggc cgggtttata
     2941 gtaattgttg ataacgttga gcctgccgta agcaccgatg ccgtcggcag tgcgattcca
     3001 ataattgaaa acgacgttgt tgatgtagtt gaaggcactg ctgctgatgg aggggttgcg
     3061 tccgacgttg gaagcaaaaa gattgcgtac caccaggcat tcgttgccgc ccagggtgcc
     3121 gccgaaggcg tggttgtagg tgtcgagtcc ttcggcaaat atggagtttt gtatggtcat
     3181 gttgacggtg ggcattttgg tgcctatgcc ggttgaatcg cgattataca tatggcgata
     3241 gatggaaata acttcgtcca gtccccagga acccgagcaa tgatcgagga taatattgcc
     3301 tacgggattg ccgcccagtg catcgttgcg gtaatagacg tcggtagagc ctctgcggaa
     3361 gcgcatatgg cgaatgataa cgtcgtgggt gtcgatttcg aaggtttctc cggcaatgca
     3421 gacgccgtcg cccggcgcgc tttgtccggc tatggtcagg taaggagccc tgacgtgtat
     3481 gggcgatttc agatgaataa cgccggatac gttgaataca atgatgcggg ctccgccttg
     3541 ttcacaggcc caacgaaaag agccttcgcc cgagtcgttg aggttggtta cagtgattac
     3601 ccgcccaccg cgtccaccgg cagtataagc tccgggcccg accgctccgg gaaaggcggg
     3661 gatggcggct ttggtcaggt gttcgggacg agaagcccag ggtacatagg gccggccggc
     3721 ttcggcctcg gccagcacta taggcagagc tttttgccag gccgaatcgg aaagacgttt
     3781 gatttcagcg tcttgggcca aacctctggc cctggtggta tcgggaacta cggggtactg
     3841 tccgtacatt ctgttgttga agcctgacag catcagaaga gccagcaagg ctgttaagca
     3901 taatttttta ttcattgaag tctgtgataa atgggtttag agaacgagcc gaagttaatt
     3961 tccttttgga aacgactgag cctgatttac ttcagcgaat gtatgtataa ttctcagcag
     4021 caagcaactg tttgttaatt atttgtgata tttaacgtgt tttaggccag gcgggcagca
     4081 ctgcaaaaaa agcagcaagc tctcatcggg cttgctgccg ggaaaagata gttcgccgtt
     4141 tgatctgaat cagatcatgg taatagtaaa caccagcttc acctggtaag ttttgttgtc
     4201 gggcgcttga tagcggaaac cctgacgtat ggtgtacttg cttcccttgg ggcaacggcc
     4261 gggatattgt cctatgttga aggtataact gttctcctga tactcggaat aaacataact
     4321 gcctgcaccc caattgatca cgttgccttc tgccgagaac caatgtccgt agccattggc
     4381 tgtatagttg ccggcaatag taccgttggc gttgaagcct acaaagcgga gtttattgcc
     4441 cagttccgtt ctgagttgtg ttccggtcac tcccaaggcc atgcaaaccg gatcgatatt
     4501 gatgcttaag ggggttgaca ggtaagaatt ggaggagtaa ggcaattcta cctcgtaata
     4561 cagtgtagtg tcgtaacgct ggtaatctcc ggagaagtcg tattggccgt acaggttggt
     4621 ctgttcaaat tttactttgt aaggccaatg ttcgtcgttg ctgtcgtcgt cgtcccaggc
     4681 atgacgccag tgggtggtgg gagcacctgt gacaaccatc cagagcatgg agcagctatc
     4741 gggaatcgca aaatgtgccg tatcatcagc ctggttgaac atatcggaat acacccggcc
     4801 gttgttttcc ttgagagcca caaaaccgta acgccatccg gccttgtcca cattgagttt
     4861 gcgataaccg tctgccccgg ccagaccgac aaagtgagct ttgacctctt tgcccgcctg
     4921 gggaacgttg agccgtatta cgttgtaacc gtagttttcg aggcaacgtt cgggattgat
     4981 tcgccagtaa ttcagggaat cgcgtgtcat gttgacgctt tgacggccaa tgtaattttt
     5041 gccgtaatcg cggattttat cgatgtccca ggtgaccaga cgggcggcac aatcgtacat
     5101 ttcgtcgttg aaagcggtct gatctatgcc ggtaactttg gtaggcttcg acgggatctt
     5161 ccggataacg ggcgccccgc cagagtttac cgataaaatc ttctccgtgc ttcattaccc
     5221 agtaatcctg aataaaataa ttggcgtaac ggggagtttc gtgcagcatg tgtttatggt
     5281 aattatccag gtaaacgcca aaattgtagt tggtaaattt ttcgttggga tagatgtcgt
     5341 aagcctgcca gttggcgcat tgttcccaga agccgttgcc tcccgagccg ttggggccga
     5401 agccgtaacg ccagccgcaa ccgttgcggt aatcggcata ggtttggtat tgaaaggcat
     5461 gtccgatttc gtgggcaatg gtggctccaa cgggcttgca ggtgtgggtg ctcacccaga
     5521 tggcaccgat cacgtcgctg ctgcctccgc cgtaggctcc ccattcatcg ctatcccaga
     5581 gatagatgat cattttgtat ttatcggccc tgctttcgcc cgggatgata aatttcatcc
     5641 ggttcttgta gacatcgtag tagatttcgg ccttggccag cagatcgtcc acatctactc
     5701 ttttgctgcc ggcattgatg gggtcgctgc ccagtccggc ttcccaaaag cagataaagt
     5761 tttcggattc tcggcttcgt tcccagcagt atttgctcga actgttgttt aagttcaggt
     5821 tcctgagatc gtcggcctga gtgaaaatct gtttgggcgg cttttcgaac gagagatact
     5881 gaaaattgga ataaagattc gttttgtagc tgccgttttt gtatagtctg tagatacttg
     5941 aacgccacac gatactgtcg atgttgttca agtcgatgct ttcgtagctg cggtcttgtt
     6001 ttaccacata gagttccgat tgggccgggc tttgcaggcc gaagcccagc aggagtatca
     6061 atagaagatt ttttttcatt ttttctgaaa tttgaggctc ttattgtcga tttttaaaag
     6121 a
//
LOCUS       DAOSGL010000105         6053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11493_length_6053_cov_4.295765, whole genome shotgun sequence.
ACCESSION   DAOSGL010000105 DAOSGL010000000
VERSION     DAOSGL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11493_length_6053_cov_4.295765"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKY68_04505"
     CDS             <1..496
                     /locus_tag="PKY68_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH24331.1"
                     /translation="SYFDVEFNILEAEMTIGTFGQRVVVSPFIDEQKRYIGKITEVNP
                     LIDAYGQITIRARIKNHDAWLVEGMNVRVVVESDMKDMFVVPKDAVVSRDGFYVVFRY
                     QDSTAIWTYVDVLYANIDSYAISGCKAKQTRINEGDVIITSGNLNLADGTPVIPRAPK
                     SNRP"
     gene            509..3616
                     /locus_tag="PKY68_04510"
     CDS             509..3616
                     /locus_tag="PKY68_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOH24332.1"
                     /translation="MIVRNLIDRPVAVSMFIVALMVVGIVSFKYIPISLMPDIDIPQI
                     TVQVSYPGASVREMESKVLRPLRNQLMQVNGLKSIRSESRTDAGAVFMSFEPGSNIDL
                     LFIEVNEKMDRAVNAMPKEMERPKVLKASATDIPAFYLDLTIKDETTGKHGILPAAGV
                     EFAQLGDFARNIVSKRIEQLPQTAMVDISGVVSSELLCVPDEKKMKTMGISTDVLEKA
                     ISDNNITLGVLSIADGLYRYNIHFDSQLMTREDIENIYINHNGRIFQFKDLCQVIEKP
                     AKRKGLVRNGRNNAVSMAIIKQNDAQMEDLQHSIANLIKDLEEEYPNVRFQLTRDQTQ
                     LLTYSIENLKDNLLIGALLACLVLFLFIRNFRLPFLIIITIPLSLVLTLLCFHLLGIT
                     LNIISLSGLILGLGMMVDNSIIVIDNITQRWKKGANLKEVIPPAVREVFSPMLSSVLT
                     TCSVFIPLVFLSGISGALFYDQAMAVTLGLFASLLIAVLVIPVYYYLFFKNKEGVDTN
                     EAMTEGIRRWWHGLWGNKSRVKHRHFSIYKPYERILRWIMRHQYVVLGAFIVLIPLSF
                     VVYTQIEKSRLPYIEYDDALMIIDWNAGISVEENDRRVDELLSKVRDKLTTSTTMVGV
                     QDFLLAHTKEITASEAVVYLKTSSMEELDKIKTEITEYVKYRYPKAVVEFEVSGNIFD
                     MIFSGNEADLLIMLQDKNGECPTVKDLRYFIDTLTARFEGVYIPPLLTEENIRYEADI
                     ELMSFYGVSYEAVFGRLRQLVNQRDLYKISHSGSSIPVTLGSGRKESRDLLMSKVKNK
                     NGVEIPLSYIIKESKGEDFKKLYSGSGGDYYPVEVTANDATVREMVDFVRNFTKQQDT
                     FYATFAGEYYSSRQMIGELALILLVAIALLYFIMAAQFESLIQPLIILSEITIDLFFV
                     LTGLYLLGESINIMSLIGMVVMSGIVINDSILKVDTINRLRRGGMSLLRAIMTGGRSR
                     LKPILMTSLTTILAITPFLYRVDMGSALQFPLSLSIIIGMFFGTLVSLFFIPLIYYVI
                     YRKKA"
     gene            3616..>6053
                     /locus_tag="PKY68_04515"
     CDS             3616..>6053
                     /locus_tag="PKY68_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOH24333.1"
                     /translation="MDAPLKHKNFLDRIPTFSLVLIMVIFIVLGLALIPLLDVGSAPR
                     PRQGKTLNVSFSWPNTAPRVVEQEVTSKIEGLLSAVKGVEEVSSVSNLGSGSVSLTLK
                     EDVNVSAVRFEISSLLKQVAGKLPEGVSYPQLSGGNIVNESSAKPVSVNLLTYRINAD
                     MEGDQIREYIEFTLVPVLSAYEDVKEVSVSGGSNKYIEVVYDPAVLSNYGLTASAIAD
                     GIKNFIGKNNIVGDIDFIDSDQERRRITLYFATGKFTKDIGEIPLTSVNGKTIYLNNL
                     ATYEYKDKLPSRYFRVNGLNTVNMTVAVDAGANLIKLSEILRAEIERIKPNLKSGVFL
                     TLTNDASSEMQTEFNKLIKRTLLSLLILLLFVWLVSRKWKYLMIIAITLLANLATAVI
                     AYYLLDIRLHIFSLAGITVSLGMIIDSSIIMVDHYSYYHDRKSFLAILAALLTTIGSL
                     IVIFFMPEYIQRDLYDFSRIIIINLSVSLLVALLFVPALVERMHFFSRKSIGQLYRRR
                     GLIRWSKFYERYIRFTQKRKGIYFAILILAFGIPFYALPGKWEKKQAYYLAGSEKIPW
                     YQAAYNATFGSLFFQNTLKEPISKIFGGSMRLFASKLDSRTYAEKERDVILTVRAQMP
                     LGGSIHHLNEKVARLEKFLARFDEIKRFETRVESTGARVTVEFKDEHEKTYFPYYLEN
                     LVIGEVLSIGGADWSTSGVSPRGFSNSLNLGHRSDRIILSGYNYLRLYKYAEEISATL
                     SQNRRVNDLIIENVQGKEVEEMYLDFDRHRIALYQFNVGTGYRALQELLTERNLGRFT
                     SDFITGDLVLKSSQ"
BASE COUNT         1635 a         1188 c         1469 g         1761 t
ORIGIN      
        1 cagctatttt gatgtagagt tcaatattct cgaggcggaa atgactattg gcacttttgg
       61 acaacgagtt gtcgtgagcc cttttattga tgaacaaaaa cgttatatag gcaaaattac
      121 cgaagtcaat cctctgattg acgcctacgg gcaaataacc atcagggcca gaatcaaaaa
      181 tcacgatgcc tggctggtgg agggtatgaa tgtgcgggtt gtggtggaaa gcgacatgaa
      241 agatatgttt gttgtgccca aagatgcggt ggtatcgcgc gatggctttt atgtggtgtt
      301 caggtatcag gatagtacgg ctatttggac ttatgtggac gtgctgtacg ccaatataga
      361 ttcatatgcg atttcgggat gcaaagcaaa gcaaacccgg atcaacgagg gtgatgttat
      421 aataacttca ggcaacctaa acctggccga cggcaccccg gtaattcccc gcgctcccaa
      481 aagcaacaga ccttagttga attaaacgat gatagtaaga aacttaatcg atcggcctgt
      541 agcggtaagt atgtttatcg ttgcccttat ggtggtggga atagtatcct ttaaatatat
      601 ccccatatcg ctgatgccgg atattgacat tcctcagatt accgtgcagg tttcttaccc
      661 gggtgcttct gtcagggaaa tggaaagcaa agtgttgagg cctttacgca atcaactgat
      721 gcaggtgaac ggactgaaaa gtatccgtag tgaatctcgt acggatgcgg gagctgtttt
      781 tatgagtttt gaacccggca gtaacattga tctgctgttt atcgaagtaa acgaaaaaat
      841 ggacagggcg gtaaatgcta tgcctaagga aatggaaagg cccaaagtgt tgaaagccag
      901 cgcaacggat attcctgcct tttacctcga tttgacaata aaagatgaaa cgacaggtaa
      961 gcacgggatt ttgcctgcag ccggtgtgga atttgcccaa ttgggcgatt tcgccaggaa
     1021 tattgtgagt aaacgtatcg agcagttacc tcaaacagct atggtagata tcagcggtgt
     1081 tgtgagttcc gaacttttgt gcgttcccga cgaaaagaaa atgaagacca tggggatcag
     1141 tacagacgtt ctggaaaaag cgatcagtga caataacatt acgctggggg tgctgagcat
     1201 cgcagacggt ttatatcgtt acaatattca ttttgactcg caactgatga cacgcgagga
     1261 tatcgaaaat atctacatca accacaacgg ccggattttt caattcaaag atttatgcca
     1321 ggtaatcgaa aaaccggcta agcgcaaggg tctggtgaga aacggcagaa acaatgccgt
     1381 aagcatggca ataattaagc aaaacgacgc gcaaatggaa gatttgcagc atagcattgc
     1441 caatctgatc aaagatttgg aagaggaata tcccaatgtg cgttttcaat tgacccgcga
     1501 tcaaacccaa ttgctgactt attccatcga aaacctgaaa gacaacttgt tgataggtgc
     1561 gttgttggct tgccttgtct tgtttttgtt catacgcaat ttccgtttac cgtttttgat
     1621 catcataaca attcccctgt cgctagtgct gactttgttg tgctttcatc tgctgggtat
     1681 taccttaaat attatatctc tttcgggtct gattttgggc ctgggtatga tggtggacaa
     1741 ttcgataatt gttattgaca atattaccca gaggtggaag aagggtgcta acctgaaaga
     1801 ggttattccg ccggcggtta gggaagtgtt ttcgcccatg ttgagttctg tcctgacgac
     1861 ttgttccgtt tttatacccc tggtttttct gagcggcata tcgggcgctt tgttctatga
     1921 tcaggcgatg gccgtaactt taggtttgtt cgcttctttg ttgatagccg tgttggttat
     1981 cccggtgtat tattatctgt tttttaaaaa caaagaggga gtagacacga acgaggccat
     2041 gaccgagggc attcgtcgtt ggtggcatgg attgtggggc aataaatcaa gagttaaaca
     2101 tcgccatttt agcatttaca aaccatatga gcgtatattg agatggataa tgcgtcatca
     2161 atacgtagta ttgggggctt ttatcgttct gattcctttg tcttttgtgg tgtatactca
     2221 gatagaaaag agtagattac cctatataga atacgacgat gcccttatga taatcgattg
     2281 gaatgcgggg atttctgtag aagaaaacga ccggcgagtg gatgaattat tgagcaaggt
     2341 gcgcgataag ttgacgacca gcactacaat ggttggtgta caggactttc tgttggctca
     2401 taccaaagaa ataacagcct cggaagcggt ggtctatctg aaaacttctt cgatggaaga
     2461 gttagacaaa atcaaaacag aaataacgga atacgtcaag taccgttatc ctaaagccgt
     2521 cgtggaattt gaagtgtcgg gtaatatttt cgacatgatt ttttcgggaa acgaggccga
     2581 tttactcatt atgcttcagg ataaaaacgg tgaatgtcct acggtaaaag acttacgcta
     2641 ttttatagat acgctgacag cccgttttga gggagtgtat attccaccct tgcttaccga
     2701 agaaaacatc cgttacgaag ccgacattga actgatgtct ttttatggcg tttcgtacga
     2761 ggcggttttc ggccgtttaa ggcaattggt caatcaacgc gatttgtaca aaataagtca
     2821 cagtggctct tccattcccg tcactctggg aagcggacgc aaagaaagtc gcgatttgtt
     2881 gatgagtaag gttaaaaaca aaaacggcgt agaaatacct ctgtcttaca tcattaaaga
     2941 atcaaagggt gaagatttta agaaattgta ttcgggcagc ggaggcgatt attaccccgt
     3001 tgaggtgacg gccaacgatg ctactgtgcg tgaaatggtt gattttgtac gtaattttac
     3061 aaaacaacag gatacttttt acgccacttt tgccggagag tattattcga gtcggcaaat
     3121 gataggggaa ttggcgctga ttttgctggt ggccattgcc ttgctttatt ttattatggc
     3181 tgcccaattc gaaagtctga ttcagccttt gattatttta tccgaaatca ccatcgattt
     3241 atttttcgtt ttgacaggcc tgtacctttt gggcgaatcc atcaatatta tgtcgttgat
     3301 tggtatggtg gtgatgagcg gtattgttat aaacgattcg attttaaaag tggacaccat
     3361 caaccgttta agacggggag ggatgtcctt gttgcgggcc attatgaccg gaggacgcag
     3421 tcgcctgaag ccgattttga tgacttcgct taccaccatc ctggccatta cgccttttct
     3481 ttatcgggta gatatgggtt cggccttgca gtttccgctt tctttgtcga taatcatcgg
     3541 tatgtttttc ggcactttgg tcagcttgtt ttttatcccc ctgatttatt acgtcattta
     3601 tcgaaagaaa gcctgatgga tgcccccttg aaacataaaa actttttaga ccggatacct
     3661 actttttcgt tggtactgat catggtgatt tttatcgttt tgggtcttgc tctaattcct
     3721 ttgctcgatg tggggagtgc acccagaccc agacagggta aaacgctgaa tgtcagcttc
     3781 agctggccta atacagctcc cagagtagtt gagcaagaag tgacctccaa aattgagggt
     3841 ttgctttcgg ctgtcaaagg ggtggaagag gtttcgtccg tttccaatct gggtagtggc
     3901 agtgtgagtt tgaccttaaa ggaagatgtt aacgtatcgg cggtgcgttt tgagatttct
     3961 tctctgctga aacaagtggc cggcaagttg cccgaaggag tttcgtatcc tcaattgtcg
     4021 ggaggcaaca ttgtcaacga gtcttcggcc aaaccggtta gtgtcaattt gctgacttat
     4081 cgcatcaatg ccgatatgga gggcgatcag atcagggagt atattgaatt taccctagtt
     4141 cccgtattaa gtgcttacga ggatgtaaag gaggttagtg taagcggagg cagcaacaag
     4201 tacattgaag tcgtttacga tccggccgta ctctccaatt acggactgac ggcttctgcc
     4261 atagccgacg gaattaaaaa ttttatcggc aaaaacaaca tcgtaggcga tattgatttt
     4321 atcgacagtg atcaggaacg cagacgtatt accttgtatt ttgccaccgg taagtttacc
     4381 aaagacattg gcgaaatacc cttaacctcg gtaaacggta aaactattta cctgaataac
     4441 ttagctacct acgaatacaa agacaagttg ccttcgcgct atttccgcgt taacggattg
     4501 aataccgtca atatgaccgt cgccgtagat gcgggagcca atttgattaa actgtccgaa
     4561 atactgcgtg cggaaataga acgtatcaaa cccaatctga aaagcggcgt tttcctaaca
     4621 ctcaccaacg acgcttcgtc ggaaatgcaa acagaattta acaagttgat aaagcgtact
     4681 ttgctctcgt tgctgatatt gttgttgttt gtatggttgg tcagccgcaa atggaagtat
     4741 ttaatgatta tagccattac cctgttggcc aatttggcaa cagcggtgat tgcgtattat
     4801 ttgctggata ttcgactgca tattttttcg ctggccggta tcacggtttc tctgggtatg
     4861 attattgatt cgtctattat tatggttgat cattacagct attaccacga ccggaaatca
     4921 tttttggcca tcctggcagc tttgttgaca accataggtt cgctgatcgt cattttcttc
     4981 atgccggaat atatacagcg cgatttatac gatttctcga ggatcataat aatcaattta
     5041 agtgtttcat tgctggtggc tttgttattt gttcccgcct tggtggaacg gatgcatttc
     5101 ttcagccgca aaagcatagg tcaattatac cggcggcgag gtttgatccg atggagtaag
     5161 ttttacgaac gttatatccg atttacacaa aagagaaagg gaatttattt tgcgattttg
     5221 atcctggctt ttggtattcc cttttatgct ttacccggta aatgggaaaa aaagcaagcg
     5281 tattacttgg ccggaagtga gaaaatcccc tggtatcagg cagcctataa tgcgaccttt
     5341 gggagcctct ttttccaaaa cacgctcaaa gagcccatca gtaaaatctt tggcggcagt
     5401 atgcgtttgt ttgcctccaa actcgattcc cgcacctatg ccgaaaagga aagagacgtc
     5461 attttaaccg tcagagctca aatgcccttg ggtggcagca ttcatcatct aaacgaaaag
     5521 gtggcacgat tggaaaaatt cttagcccgt ttcgatgaga ttaaacgttt cgagaccaga
     5581 gtcgaaagca ccggggccag agtaacggtg gagttcaagg atgagcacga aaaaacgtat
     5641 ttcccgtatt acctcgaaaa tctggttatt ggcgaagtct tgtctatagg tggcgccgac
     5701 tggagtacaa gcggagtaag tccgcgggga ttcagtaatt cattgaattt gggtcaccgg
     5761 agcgaccgga ttattctttc cggatacaac tacttgcgtc tgtacaaata tgcagaggaa
     5821 atttcggcca ccttgtctca aaacagaaga gttaacgacc tgattatcga gaatgtacaa
     5881 ggtaaagagg tggaagaaat gtatctggat tttgatcgtc atcgcattgc attgtatcaa
     5941 ttcaatgttg gcaccgggta cagggctttg caggagcttt tgacggaaag aaacctgggt
     6001 cgttttacca gcgatttcat taccggcgat ctggtgttga aatcctctca aat
//
LOCUS       DAOSGL010000106         6049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11503_length_6049_cov_4.000167, whole genome shotgun sequence.
ACCESSION   DAOSGL010000106 DAOSGL010000000
VERSION     DAOSGL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11503_length_6049_cov_4.000167"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKY68_04520"
     CDS             <1..510
                     /locus_tag="PKY68_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOH24334.1"
                     /translation="VNLNDSGRVETKLPVAYINLDSLLLDYNYSKDLNEQLLRKRENS
                     QASYNQKARQFEQEAAEFQRKYENNAFLTQQRLQSEQQRLMQKQQELQQLDEKLSQEL
                     ANEMQKMNEQLRDTIYSYLKEFNKERNYHLIFSNTMNDNILISCDVYDVTKDVVQALN
                     ARYAGSKSK"
     gene            598..2991
                     /gene="lon"
                     /locus_tag="PKY68_04525"
     CDS             598..2991
                     /gene="lon"
                     /locus_tag="PKY68_04525"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOH24335.1"
                     /translation="MISEDESEQALNQTDLPDTLPILPLRNMVLFPGVAMPVSIGRNK
                     SLRLVREAYQKNTIIAVFTQKDAKTEDPEKEHLVPVGIVSRIIRVLEMPDNSTTIILQ
                     AIKRCVLDELTLTEPYLKGRIQIREDILPETEDRQYEALIQAIKDLSVKIIKNSSGNI
                     PQEAIFAIRNIEKPFALVNFICANFAFKIQDKLALLQTDNFKDRCYKLLDIMNKESQL
                     LELKVSIQSKAREDINQQQREYFLQQQIKTIQDELGGSAQDQDIKELKSKARNKKWSA
                     DTLKVFKKEVQKLERMNPQSPDYSTQMNYLQTLLDLPWNEYSKDNFNLNRAKRILNKD
                     HFGMDKVKERILEHLAVLKLKGDMKSPIICLYGPPGVGKTSLGKSVAESLGRKYLRIS
                     LGGLHDEAEIRGHRRTYIGAMPGRIIKSLIKAGTSNPVFILDEIDKLSRDAQGDPSSA
                     LLEVLDPEQNSTFHDNFIDIDFDLSKVLFIATANTLSTIPQPLLDRMELIEVSGYLTE
                     EKVEIAHRHLLPKELENHGLKAGQLILNKTTLRHIIEKYTRESGVRELNKKIAAVFRK
                     VAHKLASDESLTQIEIKPENLKDYLGNPLYIKDKYEGNDYAGIVTGLAWTSVGGEVLY
                     VESSLSKSKSGGKLSLTGNLGEVMKESAILALEYIKAHANDLDISPELFENYNVHIHV
                     PEGAIPKDGPSAGVTMVCSLASSYTQRKIRKQLAMTGEITLRGKVLAVGGIKEKILAA
                     KRFGIKEIILPEENRKDIDDIQPVYIKGLSFHYVRDIREVLQLALLKEKVSKAQQFQ"
     gene            complement(2998..4149)
                     /locus_tag="PKY68_04530"
     CDS             complement(2998..4149)
                     /locus_tag="PKY68_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOH24336.1"
                     /translation="MKDSSMKAGIKKLIRKLGFNKSGRPLLTRIDTYIIGKFLGTFFF
                     SIVMILAIVVVFDYNEKFDKFILHEAPARAIAFDYFLNFIPYFGVKFSPLFIFISVIY
                     FTSKMAGNSEIIAMLSSGLSFNRLLRPYFISATLLAIMTFLLSSYVIPPANKIRLQFE
                     DQYVRKVSSDYVRNVQMEIEPGVIIYMERYDDKRKIGYNFFMERYEGQQLASRMTAKR
                     IALDSLNRWRVMEYLIRDFEGLHENISMGASLDTTIMMNPAEFFITKGYSEQLTTTEL
                     RQYLDRQKRRGVANIKEYEVEYQRRFSFPFSVFILTLIGVSLASRKVRGGTGLHLGLG
                     LLISFSYIFFDTLSGTFALSGSATPFVAVWIPNAVYALIAFGLYLQAPK"
     gene            complement(4146..5273)
                     /gene="tgt"
                     /locus_tag="PKY68_04535"
     CDS             complement(4146..5273)
                     /gene="tgt"
                     /locus_tag="PKY68_04535"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOH24337.1"
                     /translation="MGFKLVARDKNSRARAGVLQTAHGEIQTPVFMPVGTAGSVKAVN
                     QQELEHQVKARIILGNTYHLYLRPGLQILKAAGGLHKFMSWNKPILTDSGGFQVYSLS
                     HRRKMKEEGVTFQSHIDGSRHLFTPENVVDTQRIIGSDIMMALDECTPGTADYSYARS
                     SMELTHRWLDRGLQRYRETEPLYGYEQLYFPIVQGCVYPDLRRQSAEYVASKDCAGYA
                     IGGLAVGEPAEKMYEMIEVVNEILPQDRPRYLMGVGTPENLLEAIERGVDMFDCVLPT
                     RNGRNASLFTGQGKLNMRNKRWATDFSPIEAEGAADIDRLYSKAYLRHLFIAEELLAL
                     QLASIHNLAFYIRLMAEARQHIINQDFTEWKKQMIDQWARK"
     gene            complement(5311..>6049)
                     /locus_tag="PKY68_04540"
     CDS             complement(5311..>6049)
                     /locus_tag="PKY68_04540"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOH24338.1"
                     /translation="YLLFQPEKTLEQVYVSGHTAIIDKDSCTNCGLCMDYCRFDAIHL
                     TEEEVQISGISCEGCFLCSRICPEQAIRMEPEDRSRLYAGNFRYGKMVYGRLFPGEEN
                     SGRLVNLVRQQARDIARKNELDLILLDGPPGIGCPVISTITGVDKVLIVTEPSVSGLH
                     DLQRIHELCRHFGLSQTVVINKHDLNPDMCTEIERYCREQGLKLQAKIPFDPQMVEAM
                     VKGQSILEYAPESPAALCIKNHLEELL"
BASE COUNT         1778 a         1507 c         1334 g         1430 t
ORIGIN      
        1 gtaaatctga atgattcagg ccgggtggaa acaaaattac ccgtcgccta catcaacctc
       61 gactcactgc ttctggatta caattattcc aaagatctta acgagcaatt gttgcgcaaa
      121 agagaaaact cccaagccag ttacaatcaa aaggcccgcc agtttgagca ggaagcggcc
      181 gaattccagc gcaaatacga aaacaacgcc tttttaaccc agcaaaggct gcaaagcgaa
      241 caacaacgcc tgatgcaaaa gcaacaggaa ttacagcaac tggacgagaa actatctcag
      301 gaactggcaa acgaaatgca aaaaatgaac gagcaattga gggataccat ttattcctat
      361 ctaaaggagt tcaacaagga gcgcaactat catttaatat tcagcaacac gatgaatgac
      421 aatatactga tctcctgtga tgtatatgat gtcacaaaag acgttgtcca ggcactcaac
      481 gctcgttatg ccggctcaaa atcaaaataa atccggttta gtggaattat tgttatgata
      541 aatctttttt caagacaact gttttcttcc gatgaggaag agggagattt tattcccatg
      601 atttccgaag acgaatcaga acaggcgctt aaccaaacgg atttaccgga cacactaccc
      661 atactgcccc tgcgcaatat ggtcttgttt cccggggtag ccatgcctgt aagtatcggc
      721 cgcaataaat ccttgcgtct ggtcagggaa gcctaccaaa aaaacaccat catcgccgtc
      781 tttacgcaaa aggatgccaa gaccgaagac cccgaaaaag aacatctggt gcccgtaggc
      841 atagtttccc gcatcatcag ggtgctggaa atgcccgata attcaaccac catcatcctg
      901 caagccatca aacgctgcgt actggacgaa ctcaccctga cggaacctta tttaaaaggc
      961 cgtatccaga ttcgcgaaga tattttaccc gaaacggaag accggcagta tgaggcatta
     1021 atccaggcca tcaaagattt atcggtcaaa attatcaaaa attcttccgg caatattcca
     1081 caggaagcca tttttgccat ccgcaacatt gaaaagccct ttgccctggt caattttatc
     1141 tgtgccaact ttgctttcaa gatccaagac aaactggcac tactgcaaac cgacaatttc
     1201 aaagatcgtt gctataagct gctggacatc atgaacaaag agtcgcagct tctggagctc
     1261 aaagtctcca tacaatccaa agccagggaa gacatcaatc aacagcaaag agaatacttt
     1321 ttgcagcagc agatcaagac tatacaagac gaactcggag gcagtgccca agaccaggat
     1381 atcaaagaat taaaaagcaa agccagaaac aaaaaatggt ctgccgatac cctgaaggta
     1441 tttaaaaagg aagttcaaaa gctggaacgc atgaatccgc aatctccgga ttattcaacc
     1501 caaatgaatt accttcagac cttgctcgat ttaccctgga acgaatactc caaagacaat
     1561 ttcaatctaa accgcgccaa aagaatactc aacaaagatc attttggcat ggacaaagtg
     1621 aaagaacgca ttttggaaca tcttgccgta ctcaaactca agggcgacat gaaatcgccc
     1681 attatctgcc tgtacggccc tcccggagtg ggcaaaactt ccctgggtaa atctgtggcc
     1741 gaatcgctgg ggcgcaaata cctgcgtata tctctcggag gcctccacga cgaagccgaa
     1801 ataagaggtc acagacgcac ttacatcgga gccatgcccg gccgcataat caaaagtctg
     1861 attaaagcag gcacatccaa tccggttttt attctcgacg aaatcgataa actaagccgt
     1921 gacgcccagg gcgacccttc gtcggcttta ctggaagtgc tcgatccgga acaaaactct
     1981 acttttcacg ataatttcat cgacatagat ttcgacttgt ccaaggtctt gtttattgcc
     2041 actgccaaca cattgagtac cattcctcaa cccttgctcg accgcatgga gctcatagag
     2101 gtttcgggct acctgaccga agaaaaagta gaaattgccc acaggcatct cctgcctaag
     2161 gaactcgaaa accacggttt aaaagccggt caattgatcc tgaataaaac caccctccgg
     2221 catatcatcg aaaaatacac ccgggaatcg ggagtgcgtg aactgaacaa aaaaatagcc
     2281 gccgtcttta gaaaagttgc tcataaactt gcttcggatg aatcgcttac ccagatcgag
     2341 atcaagccgg aaaacctgaa agattacctt ggcaacccgc tctacatcaa ggacaaatat
     2401 gaaggcaacg attacgccgg tatagtcacg ggactagcct ggacatcggt gggcggcgaa
     2461 gtgctttacg tggaatccag cctgagtaaa agcaaaagcg ggggcaaact cagtcttacc
     2521 ggcaacctgg gcgaagttat gaaagaatcg gccatattgg ctttggaata catcaaggcc
     2581 catgccaacg atctggatat ttctcccgag ctgtttgaaa attacaacgt ccatatccac
     2641 gttcccgaag gcgccatccc caaagacggt ccctcggccg gtgtaaccat ggtttgctcc
     2701 ctagcctcgt cctatacgca acgcaaaatt cgtaaacaac tggccatgac cggcgagatt
     2761 accctgagag gtaaagtgct ggccgtgggc gggatcaaag aaaaaattct ggcagccaag
     2821 cgctttggaa taaaagaaat cattttgccc gaagaaaacc gcaaagacat cgacgatatt
     2881 cagcccgtct acatcaaagg cctcagtttt cactacgtaa gagatatccg cgaagtgttg
     2941 caactggctc tgctcaaaga gaaagtgagc aaagctcaac aatttcaata atactgtcta
     3001 tttgggagct tgcaggtaaa gcccgaaggc tatcagcgca tacacggcat tgggaatcca
     3061 caccgccaca aaaggggtgg cggaacccga gagagcaaag gttcccgaca aggtatcgaa
     3121 aaatatatac gaaaagctga tcagaagccc cagccccagg tgcaaaccgg tgcctcccct
     3181 caccttgcgc gaggccagcg acacaccaat cagcgtgaga ataaacaccg agaaaggaaa
     3241 ggaaaaacgt cgctgatatt ccacctcata ttccttgata ttggccactc ccctacgttt
     3301 ttgcctgtcc aggtattgcc tgagttcggt ggtagtcaac tgttcactat agcctttggt
     3361 tataaagaac tccgccggat tcatcataat agtggtatcc agactggcac ccatgctgat
     3421 gttttcatgc aatccttcga aatcgcgtat caggtattcc attactctcc aacgattgag
     3481 cgaatccaga gcgattcgct tggccgtcat tctggacgcc agttgctggc cttcgtagcg
     3541 ttccataaaa aaattgtatc cgattttcct cttatcgtcg taacgctcca tatagatgat
     3601 tacgccgggc tcgatttcca tctgtacatt gcgaacataa tcggaagaaa ctttgcgtac
     3661 atattgatct tcgaattgca gacgtatttt gttggcaggc ggaataacgt aagaactcag
     3721 caaaaaagtc attatggcca acaaagtagc cgaaataaaa taaggtctga gcaagcggtt
     3781 aaaactcaag ccgctggaga gcatggcaat gatttcggaa ttgccggcca ttttcgaagt
     3841 aaaatagatg acggaaataa aaataaagag cgggctgaat tttactccga aataaggtat
     3901 gaagttcaga aaataatcga atgctatggc tctggccggg gcttcgtgta gaataaactt
     3961 gtcgaatttt tcgttgtagt cgaacaccac cacgatggcc agaatcataa caatggaaaa
     4021 aaagaaagtg cccagaaact tgccgatgat ataggtatct atacgcgtca acaaaggcct
     4081 tccggattta ttaaatccca gtttccgaat tagcttttta ataccggctt tcattgaact
     4141 gtctttcatt ttctggccca ttgatcgatc atctgctttt tccattccgt gaaatcttgg
     4201 tttataatat gttgccgggc ttccgccatc aagcggatat aaaaggccag attatggatc
     4261 gaggccagtt gcagggccaa caattcctcg gcaatgaata aatgcctcag gtaggctttg
     4321 gaatacaaac ggtctatgtc tgcggcaccc tctgcttcta tgggagaaaa gtcggtagcc
     4381 caacgtttgt ttcgcatatt gagcttgcct tgcccggtga acaaagaggc attgcggcca
     4441 ttgcgggtag gcagcacaca atcaaacata tccactcccc gctctatggc ttcgagcaag
     4501 ttttcgggtg tacccactcc catcaaataa cggggacgat cttgaggcag aatttcattg
     4561 accacctcta tcatctcgta cattttttcg gctggctctc ccacagccag gccgcctatg
     4621 gcataacccg cacagtcttt ggaagcgaca tattcggccg attgacgcct taaatcggga
     4681 tacacacagc cctgcactat ggggaaataa agctgttcgt agccatacag cggttctgtt
     4741 tcgcggtaac gttgcaggcc gcgatccagc cagcgatgcg tcagttccat cgagctacgt
     4801 gcatagctgt aatcagcggt tccgggtgta cactcatcga gcgccatcat gatatccgaa
     4861 ccgataatgc gctgggtatc taccacattt tcgggggtga ataaatgccg ggagccgtcg
     4921 atatgcgact gaaaagtgac tccctcctct ttcattttgc ggcgatgcga gagcgaataa
     4981 acctgaaaac caccactgtc ggtgagaata ggcttgttcc aggacataaa tttatgcaga
     5041 ccgccggctg ctttgagtat ctgcaaaccc ggacgcaaat acaaatgata ggtattgccc
     5101 aaaataatcc gggcttttac ctggtgttcc agttcctgct gattgacggc tttgacgctg
     5161 ccggcagtgc ctacaggcat aaaaacagga gtctgtatct ctccgtgagc tgtttgcagt
     5221 acgccggctc tggcacgact gtttttgtcc ctggctacta atttaaaacc catgcgcctg
     5281 aatttgaggc aaagataata ttttgcccac ttaaagcagt tcttccaggt ggttttttat
     5341 gcaaagggct gccggcgatt cgggagcata ctccagaatg ctttgtcctt tgaccatggc
     5401 ttctaccatt tgcggatcga aaggaatttt agcctgcagc ttcaggcctt gctcccggca
     5461 atagcgttct atctcagtac acatatccgg attaaggtcg tgtttgttta taactaccgt
     5521 ctgactaagc ccgaaatgtc gacacaactc gtggatacgt tgcaaatcgt gcaagcccga
     5581 cacactgggt tcggtgacaa taagaacctt atccaccccg gtaatggtag aaatcaccgg
     5641 acaaccgata ccgggaggtc cgtccagtaa gataagatcc aactcgtttt tgcgggcaat
     5701 atccctggcc tgctgcctga ccagatttac caggcgaccg gaattttctt caccgggaaa
     5761 caacctgcca tagaccatct ttccgtaacg gaaattgcct gcgtacaagc ggctcctgtc
     5821 ttcgggctcc atacggatag cctgctcagg acaaatccgg gaacaaagaa aacatccttc
     5881 gcaggaaatc ccggaaattt gtacttcttc ctccgtcagg tgtatggcat cgaaacggca
     5941 ataatccata caaagtccgc agttggtaca agaatccttg tctatgattg ctgtatgacc
     6001 cgaaacatag acttgctcga gggttttttc gggttggaac aaaaggtaa
//
LOCUS       DAOSGL010000107         5955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11752_length_5955_cov_3.897119, whole genome shotgun sequence.
ACCESSION   DAOSGL010000107 DAOSGL010000000
VERSION     DAOSGL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11752_length_5955_cov_3.897119"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(379..1758)
                     /locus_tag="PKY68_04545"
     CDS             complement(379..1758)
                     /locus_tag="PKY68_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOH24339.1"
                     /translation="MKNQSELAFDAGIRIEVADRRIFVKMPKRDADVQFVRSFRFAYW
                     DAGAFLWVIPHYRNNLELLKEYFAERLVECKEMKALDAKGSVTAKIKTNNSELLVIQT
                     STKQLRLIVAFNPEFNRLIRELPYSRWDAKNRWWTAPFSDKIINRLKQIALDLGLQLR
                     LEQEEQGNKGKPRLSPFDVPNYRPCPETYELKLRELRYSEHTIRSYTNLFEEFINYYH
                     HFDIDRIDETMITAFLRYLVIERKVSISYQNQAINAIKFYYERVLGGQRKVYLVDRPR
                     TETKLPVVLSQEEVCEILKATVNLKHKAILMTIYSAGLRISEAIQLKVSDIDSQRMQI
                     RITQAKGKKDRYTLLAVKTLDLLRQYVRMYKPKFWLFEGQKGEQYSNSSIQAILKVSL
                     SKTKIQKRVTVHTLRHSFATHLLESGTDLRYIQNLLGHGSSKTTEIYTHVTTRGFEQI
                     KSPLDKLNL"
     gene            complement(1989..3614)
                     /locus_tag="PKY68_04550"
     CDS             complement(1989..3614)
                     /locus_tag="PKY68_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24340.1"
                     /translation="MKTFSHLFLYLLLAALGFSCSNFFEVESKAYLDIEKNRLDTPND
                     SVYSVFGLLSGMQDIGPVCVLLGELQADLMDLTPNADADLKELNEHRASVDNPYLKAT
                     GFYRIINDCNYMIQHMDTVTGDKALLQDYAAAVAIRAWTYLQMSKIYGPVPYFDHCIL
                     DAEEALTADFPKMSPAELLPLLIAQVQDILHIPRPFRRMNSIPVGKMIPGNEFLLGEC
                     YLWLNDYENAALWYKRAIEKDNYRFVCNNSFTIAYEDDGSIVQSWYQIFENSDYAHTY
                     ENNASIIYDKNYGKSNSLRSLYFGKEGSNKIKPSSAIIEDWIAQPLGTTNLELKKLGD
                     RRGLTNAWRFDKEGPENTIRPLINKFASDSIEVNIIIERAAIYHLRYAEAINRTGRPG
                     LALQMINKGPRPLYLNDSTLVKRQELAGKPEIVDFDHAAYATSMGIRGRVDLEPYTFD
                     AALPTLEDSVLFVEDKILQEAALELAFEGHRWFDLVRVAQRRPNPAAFLSGMVCRKFE
                     ASIDPLFPFEASADKATVKAAIESAGFFLPLPE"
     gene            complement(3625..>5955)
                     /locus_tag="PKY68_04555"
     CDS             complement(3625..>5955)
                     /locus_tag="PKY68_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24341.1"
                     /translation="EVVSAPRQIHVLQSKDYRAYALEQIGGAGYTEQEIQDQLYLSDD
                     PSRPYYHTFHTNTNWQDEIFQNATVQRYYGQVTGGDDIAGYAISIGYDDAESVMKTGS
                     MNRFSSRFNSDIKFTERLLFALGLSFSQVNKQLRDDGCVQLSSPTYLSLIKSPLVSPY
                     LLGEDGSRLPTDADEDFWGLSNPVSILLRGLGENSQQRVDLNGKLEYSLNKQWMLTGL
                     FGYNTDKLTESYFLPDYGVASVELERIRQVSRNYVQNRSARYIGVYTNLNAQYRGVWN
                     YIHQLRLNAGLRYHSNQYLSQGGSGYNTVEDKFIYLTNNLLAKAIFNENNRWLWGSAY
                     LDAQYTLKNTFDFSFNLSADASSRTGSKHRSGVFSSAAASWLLSSEKFMADARAIDLL
                     KLRISAGRNGSDDLGPANPRGYFGSVSYMNMMGLSLRNLNNEDLKYETTDKANLGLDL
                     AVFNERLALSVDLYQHLTHDLITMRDAKQVSGFDYVWVNEGSLQNRGLEASLNLRLVD
                     TRKFKWNLQAGLGHYKNRLKDLANDIFTEYSGATVLSRQGNPLGVFYGYRTQGVFSTY
                     EEAQAANLKTLSNNSTVYQFKAGDIHFADLNNDGWIDEKDRVIIGDPNPDFYGSLSTR
                     LSYAGFSLDAVFSGVYGNDIYNHLRAGMEAMDSFNNQSASVLNRWKGQGHAADIPRAA
                     YNDPGGNARFSDRWIEDGSYLKLQKLGLEWAAPGEVLFLDGLRVFIYAENLVTWTNYL
                     GRDPDLSHSNYSLLQGIDRGYLPQSRSFMAGIKINL"
BASE COUNT         1449 a         1338 c         1533 g         1635 t
ORIGIN      
        1 tgaaagcata attgtcaaaa aggctgtaat cgttaaaatc tttctcatat tatttaaatt
       61 taaatgtttg cttactctat acggttttca gcatctccgt gcgttttaaa gttaccgcca
      121 acacccaaat aaccctattg ggtttatttc cgctttattc ccagatccgg ggctatcaaa
      181 aaagtggctg cgatagtctg ttgtgggcaa agaataatcc atgtgtaatt ggagtgtttc
      241 gggttattat ggtattaatt atctgtcaat tagctttttt caaagcaagt aagtctaata
      301 aacagcttgc tttgggttcg gtaatcgact aacaaaaata cacattaatt gtattttcac
      361 atctgttttc gaagattttt acaagttcaa tttgtccaag gggcttttaa tttgctcaaa
      421 gcctctggtg gtgacgtgtg tataaatttc agtagtcttg gagcttccat gtcctaacag
      481 gttttgaata tagcgtaagt ctgtaccgct ctccagcaag tgggtggcga aactatgtcg
      541 cagggtgtgt actgtgactc ttttttggat ttttgtttta gagagtgaaa ctttcaaaat
      601 ggcttggata ctactgttcg aatattgttc ccctttttgt ccttcaaaca accagaattt
      661 tggtttatac atgcgaacgt attggcgcaa aagatctaat gttttaacgg ccaataaggt
      721 gtaacggtct tttttacctt tggcctgggt aattctgatt tgcatgcgtt gtgaatcaat
      781 atcactgact ttcagctgaa tcgcctcact gatacgcagg ccggcagaat aaattgtcat
      841 taagattgcc ttgtgtttca ggttgactgt ggctttcaaa atttcgcaaa cctcttcctg
      901 actgagcact accggcaatt tggtttccgt tctggggcgg tcgaccagat agacttttcg
      961 ctgtccgcca agaactcgtt cataatagaa cttgatggca tttatcgcct ggttctgata
     1021 ggatatcgat actttacgtt caatcaccag ataacgtaaa aaagccgtga tcatagtttc
     1081 gtcgatgcgg tcaatgtcaa aatggtggta ataattaatg aactcttcaa aaagattggt
     1141 atagcttctt atagtatgtt cgctgtagcg aagttcccta agtttcaatt catatgtctc
     1201 cgggcagggg cgatagttgg gcacatcaaa aggactaagg cgtggtttgc ccttatttcc
     1261 ctgttcttct tgttccaggc ggagctgaag acccagatca agggctattt gtttcagtct
     1321 gtttataatc ttatcggaaa aaggtgctgt ccaccaacgg tttttggcat cccagcgaga
     1381 atagggaagt tctctgatta aacgattgaa ttccggattg aaggcaacta ttagccttaa
     1441 ttgtttggta gaggtttgga taaccaggag ctcactattg tttgtcttta tttttgcagt
     1501 tacagatccc ttagcatcta aagctttcat ttccttgcat tccacaagac gctcagcaaa
     1561 atattctttc aatagctcca aattgtttct gtaatgggga ataacccaaa gaaaggcgcc
     1621 ggcatcccaa taggcaaagc gaaaagaacg aacaaattgt acatcggcat cccttttggg
     1681 catttttacg aagattcgcc tatcggccac ttcaatacga atccctgcat caaaagcaag
     1741 ctcagactgg tttttcatat aaatcacata ctctgatgat taaactttga aatattgtgt
     1801 atatatgcca aaaacagcaa ataacaccaa ctcaaaaaca gagtaaagat acatataatt
     1861 aagacgtaat taatgcatat ttaatgtttt tagagatgaa accgaatgaa acaccagccg
     1921 tggataccgg gaggttgaac ggtaggccgt tgagtgaaag caagcgctct cggaaaacga
     1981 aaaccggctt actccggcag tggcaaaaag aagccggccg attcaatggc tgctttcacg
     2041 gtggctttgt ctgccgaggc ttcgaagggg aataggggat cgatgctcgc ttcaaatttg
     2101 cggcaaacca ttcccgataa aaaggcggcg gggttgggtc ggcgttgggc tacacgtacc
     2161 agatcgaacc agcgatgtcc ttcgaaggcc agctccaagg cggcttcctg caagattttg
     2221 tcctctacaa acaaaacgga gtcttccaga gtgggcaatg cggcgtcgaa ggtgtagggc
     2281 tccagatcca cgcgtccgcg gatacccatg gaggtggcgt aagcggcgtg atcaaagtcc
     2341 acgatttcgg gtttaccggc cagctcttgg cgcttgacca gggtgctgtc gttcagatag
     2401 agggggcgag ggcctttgtt gatcatttgc aaggctagcc ccggacggcc tgtgcggttg
     2461 atggcttcgg cgtaacgcaa atgatatatg gctgctcttt cgatgatgat attcacttcg
     2521 atggagtcgc tggcaaattt gttgatcagc ggccggatgg tattttcggg gccctccttg
     2581 tcgaatctcc aggcattggt caggcctctg cgatcgccca gtttcttcaa ttcgagattg
     2641 gtggtgccca aaggttgagc aatccagtct tcgataatgg ccgacgaagg tttgatcttg
     2701 ttgctgcctt ccttgccaaa gtacaaagac ctcagactgt tggatttacc gtagttttta
     2761 tcgtaaatga tggaagcatt gttttcgtaa gtatgggcat agtcggaatt ctcgaagatt
     2821 tgataccagg attgaacaat actgccgtcg tcctcgtagg cgatggtgaa gctgttgttg
     2881 cagacaaagc ggtaattgtc tttttcgatg gcccttttgt accacaaagc agcgttctcg
     2941 taatcgttga gccacaaata acattctccc agcaaaaact cgttgcccgg aatcattttg
     3001 cccaccggaa tgctgttcat ccggcgaaaa ggcctgggaa tatgtagaat atcctgtacc
     3061 tgagcaataa gcaggggcag gagttccgcc ggactcattt tgggaaaatc ggcggtgagc
     3121 gcctcttccg cgtcgagaat gcaatggtcg aaataaggca cagggccgta gatcttactc
     3181 atttgcaaat aggtccaggc tctgatggcc acggctgcgg cgtaatcctg caacaaagcc
     3241 ttgtcgcctg tcaccgtatc catgtgctga atcatatagt tgcagtcgtt gatgatacga
     3301 taaaagccgg tggctttcag gtagggatta tcgacgcttg ccctgtgttc gttgagctct
     3361 ttgaggtctg catcggcgtt gggagtcagg tccatcagat cggcctggag ttcacccaaa
     3421 agaacgcaga ctgggccgat gtcctgcatg cccgacaata aaccaaacac ggaataaacc
     3481 gagtcgttgg gcgtgtccag ccggtttttt tcgatatcga ggtaggcctt ggattcgact
     3541 tcgaaaaagt ttgaacagga aaaacccaaa gcagccagga gcaagtatag gaagaggtga
     3601 gaaaaagttt tcatagttcg ttgcttagag attgattttg ataccggcca taaacgatcg
     3661 ggactgaggc aggtaacccc ggtcgatgcc ttgcagcagg ctgtagttgg aatggctcag
     3721 gtccggatcc ctgcctaggt aattggtcca ggtgacgagg ttttcggcgt agataaacac
     3781 acgcaggccg tcgaggaaga ggacttcgcc gggagccgcc cattcgaggc ccagtttctg
     3841 cagcttcagg taggaaccgt cttctatcca gcgatccgag aagcgggcgt tgcccccggg
     3901 atcgttgtag gcggccctgg gaatatcggc agcgtggccc tggcccttcc agcgatttag
     3961 cacgctggcc gactggttgt tgaaactgtc catagcttcc atcccggctc tgaggtggtt
     4021 gtagatgtcg ttaccgtaca ctccgctgaa taccgcatcg aggctaaagc cggcgtagct
     4081 caggcgggtc gataggctgc cgtaaaaatc gggattgggg tcgccgatga ttacacggtc
     4141 tttttcgtct atccagccgt cgttgtttag atcggcaaaa tgtatgtcac cggccttgaa
     4201 ttgatagacc gtactgttgt tggacaaagt cttgagattg gcggcctgag cttcttcgta
     4261 ggtgctgaag actccctggg tccggtaacc gtaaaatacg cccaggggat tgccttgccg
     4321 cgagagtact gtagctcccg aatattcggt aaaaatatcg ttcgctaagt ctttcagcct
     4381 gtttttataa tgccccagac cggcttgcag gttccatttg aacttgcggg tatccaccaa
     4441 cctcaggttt agagaagctt ccagtccccg gttttgcagt gaaccttcgt ttacccagac
     4501 gtaatcgaag cccgacactt gtttggcatc gcgcatagtg attaaatcgt gggtaagatg
     4561 ctgatacagg tcgaccgata aagccagcct ctcgttaaac acggccagat ccagacccag
     4621 atttgctttg tcggtggttt cgtatttgag atcttcgttg ttgaggtttc tcaggctcag
     4681 ccccatcata ttcatgtaac tgaccgagcc gaaatagccg cggggattgg ccggacccag
     4741 atcgtcgctg ccgttgcggc cggcgcttat tctgagtttc agcagatcga tagcccgggc
     4801 atcggccatg aatttttcgg aagaaagcaa ccaggaagca gcggccgaag aaaagactcc
     4861 gctgcggtgt ttgctgcccg ttctggagct ggcgtcggcc gagaggttga acgaaaaatc
     4921 gaaggtgttt ttcagcgtgt attgagcatc caaataggcc gaaccccaga gccatcgatt
     4981 gttttcgtta aagatagctt tggccagtag gttgttggta aggtagatga atttatcttc
     5041 cacggtgttg tagcccgagc ctccctgtga gagatattga ttggaatgat aacgcagccc
     5101 cgcgttaagc cttagttgat gaatgtaatt ccagactccg cggtattggg cgttcaggtt
     5161 ggtatacacg ccaatgtagc gggcagaacg gttttgaacg taatttctgg acacttgcct
     5221 gatgcgttcc agttctacac tggccactcc gtagtcgggc agaaaatacg attcggtcag
     5281 tttgtcggtg ttgtagccga aaagtccggt taacatccat tgcttgttta ggctgtattc
     5341 cagcttgccg ttcaggtcga ccctttgctg agaattttca cccaggcccc ggagcaggat
     5401 agataccggg ttactgaggc cccagaaatc ttcgtcggca tcggtgggca ggcggctgcc
     5461 gtcttcgccg agcaggtagg gcgataccag gggcgatttg atcagcgaca ggtaagtagg
     5521 cgaggagagt tgcacgcatc cgtcgtcgcg caattgcttg ttcacctggc taaacgacaa
     5581 tcccagcgca aagagcaaac gttcggtaaa cttgatgtcg ctgttgaagc gggaactgaa
     5641 gcggttcatg ctgcctgttt tcataacgct ttccgcatcg tcgtagccta tggaaatggc
     5701 gtaaccggca atgtcgtcgc caccggtcac ctgaccgtaa taacgttgaa ccgtagcgtt
     5761 ttgaaaaatc tcatcctgcc agttagtgtt ggtgtggaag gtatggtaat agggcctgga
     5821 cggatcgtcg ctcaggtaga gctgatcctg aatttcctgt tcggtatagc cggcaccgcc
     5881 gatttgttcc agggcatagg cccggtaatc tttgctctgc aggacgtgaa tttgcctggg
     5941 agctgaaacc acttc
//
LOCUS       DAOSGL010000108         5947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11781_length_5947_cov_3.768669, whole genome shotgun sequence.
ACCESSION   DAOSGL010000108 DAOSGL010000000
VERSION     DAOSGL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11781_length_5947_cov_3.768669"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKY68_04560"
     CDS             complement(<1..338)
                     /locus_tag="PKY68_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOH24342.1"
                     /translation="MAIIPGNDLKTHIGIFGRMNVGKSSVINALTGRKQAIVSAVPGT
                     TTDPVRKSMELGGLGPCVLIDTAGTDDSTELGRQRVDKSLEVLSEMDLALILFSENKL
                     EEPELLLLER"
     gene            complement(355..1779)
                     /gene="hydG"
                     /locus_tag="PKY68_04565"
     CDS             complement(355..1779)
                     /gene="hydG"
                     /locus_tag="PKY68_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOH24343.1"
                     /translation="MMFNPQKYAIPDRRMQPFIDPDEIRACLQNARPDARAVRDTIAR
                     SLSRERLSMQETALLLNAEDPELIEEIKEGARQLKREIYGNRIVLFAPLYIGNKCIND
                     CVYCGFRASNREAVRKTLSDEEIVQEIASLEDQGQKRLILVYGEHPDYDAHYIAHTVK
                     LAYGVKKGRGEIRRVNINAAPLDIEGFRVVKTAGIGTYQIFQETYDPQAYSLYHRGHK
                     ADYAYRLTALDRAQEAGIDDVGIGALFGLYDWKFEVLGLLRHTNHLEACYKVGPHTIS
                     FPRIKDASARQVDGRYFVSDSDFVRLVSILRLAVPYTGLILTAREPAALRDELIQFGV
                     SQIDGGTKIEIGAYAEAQNRHKDLRRGQFLINDDRPLNDIIDELISMDMIPSFCTSCY
                     RMGRTGEHFMEFSVPGFIKRYCTPNAILTLAEYLLDYAPEHTSRKGWELIEREMARMD
                     EGPVKKKLEQKLELLKSGQRDCYF"
     gene            complement(1845..2924)
                     /locus_tag="PKY68_04570"
     CDS             complement(1845..2924)
                     /locus_tag="PKY68_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOH24344.1"
                     /translation="MEKHSKIYVAGHRGLVGSAIWNNLLSKGYTRLLGRSHAELDLME
                     GPAVEAFFAAEQPEYVVLAAAHVGGIQANNVYRADFIYRNLQIQQNIIGAAYRHKVKK
                     LLFLGSTCIYPRDAAQPIREEALLTGPLEYTNEPYAIAKIAGLKLCESFNLQYGTNFI
                     AVMPTNLYGPNDNFDLERSHVLPAMVRKIFLASCLLRGDETLLRRDLDSRPIEGFDGK
                     TPLPEIKILLQKYGIYPDRLELWGTGKPLREFLWSEDMADACVFIMERVDFEDLRPKD
                     TSDEVRNCHINIGTGKEISIAALAAMIAEKTGYAGRISFDASKPDGTMRKLCDVNKLH
                     KLGWQHRVDISEGLDRILDWYRRSL"
     gene            complement(2935..4014)
                     /gene="gmd"
                     /locus_tag="PKY68_04575"
     CDS             complement(2935..4014)
                     /gene="gmd"
                     /locus_tag="PKY68_04575"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOH24345.1"
                     /translation="MTKVALITGITGQDGSFLAEFLLEKNYEVHGTIRRSSSFNTGRI
                     EHLYLDEWVRDMKRKRLINLHYADMTDSSSLIRVIQTVKPDEIYNLAAQSHVKVSFDV
                     PEYTVESDAVGTLRLLEAVRILGLEKSTRIYQASTSELYGLVQEVPQKETTPFYPRSP
                     YGVAKQYGFWITKNYREAYGMFAVNGILFNHESERRGETFVTRKITLAAARIAQGHQD
                     KLYLGNLSARRDWGYAKDYVECMWLMLQHHTPEDFVIATGQMHTVREFCTLAFEEADI
                     SLRWEGEGVEEKGIDIKSGKVLVEVDPKYFRPAEVEQLMGDPSKAKKLLGWNPTRTSF
                     EDLVKLMVKHDMRFVKKLYLKSQLD"
     gene            complement(4103..5380)
                     /locus_tag="PKY68_04580"
     CDS             complement(4103..5380)
                     /locus_tag="PKY68_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH24346.1"
                     /translation="MKKRNILLIFLVFIGVITFLDRINISVAGTSIMADLGLTHKQWG
                     WVLSAFIISYGLLQLPLGLLGDRKGQRKVLAVIVLWWSVFTGLTGMAGGFISLLFIRF
                     AFGIGEAGAYPCMTGAIGRWFPKSENARAQGYIWAASRVGGALTPFIVIPVIVHLGWR
                     EAFYILGALGVVWVIAWYWWFRDNPADKKGITPAELAELDDKPKVEVAKQKVPWGAIL
                     HSKQFWLILAMYFFYAWGSWFFFSWFPTFMEEGRGFSKAALTYAVAVPFLMSVAGNIT
                     GGYLSDRFSKKYGLKKGRRLLGVGGLAASAVFMFLAAFIPGKMQVFIFLSLCFGVIDL
                     MLPSAWAICLDIGKQYAGAISGAMNTAGNIGGFVCATIFGYLVSGTGNYNFPLFVIAG
                     MLVISALLFTRIDASKPLLPEDQAEPLPQKTEK"
     gene            complement(5387..>5947)
                     /locus_tag="PKY68_04585"
     CDS             complement(5387..>5947)
                     /locus_tag="PKY68_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOH24347.1"
                     /translation="HIADDSLIQEVIAEGRVTPAVKRRMLACYYAAVDAGADYIFNTC
                     SSVGDVADTARFFMPVPLIRIDEQMALKAISLSARIGVLATLPTTLQPTADLLERLAA
                     QQGKKIELVDGLAKGAFQAAMGGNPQKHDELILEASKTVAPKVDVIVLAQGSMARMEE
                     KLAAETGKTVLSSPLLGVLGLKKLIG"
BASE COUNT         1487 a         1591 c         1450 g         1419 t
ORIGIN      
        1 caacgttcca gcaacaagag ttcaggttct tccagcttgt tttcgctgaa aaggatcaaa
       61 gccagatcca tttccgacaa aacctcgagg cttttgtcca ccctttgccg gcccaactcc
      121 gtcgaatcgt ccgtaccggc cgtgtctata agcacacaag gacccagccc gcccaattcc
      181 atagatttgc gcaccgggtc ggtggtggtt ccgggtacgg ccgacactat ggcttgtttt
      241 cggccggtca gggcattgat aacggaactt ttgcccacat tcatccggcc aaagattccg
      301 atatgagttt tcaggtcgtt tccggggata atagccatat ccagagcttt gaacttaaaa
      361 ataacaatcg cgttgtcccg acttcaacag ctccagtttt tgttccagct tcttttttac
      421 gggaccttcg tccatccggg ccatctcccg ctctatcagc tcccagcctt tgcgggaagt
      481 gtgttcgggc gcgtaatcca acaggtactc agccagggta agtatggcat tgggggtgca
      541 atagcgcttg ataaagcccg gcaccgaaaa ctccataaaa tgttcgcccg ttctgcccat
      601 acggtaacat gaggtacaaa aagaaggaat catatccatg cttatcaatt catcgataat
      661 atcgttgagc gggcggtcgt cgtttataag gaactgaccc cggcgcaggt ctttatgccg
      721 gttctgcgct tcggcataag ctccgatttc tattttggta cctccgtcta tctgcgacac
      781 cccgaattga atcagttcat cgcgcaaagc cgccggttcg cgggcggtta gaatcagtcc
      841 ggtataaggc actgccaggc gaagaataga caccaaacgg acgaaatcac tgtcggacac
      901 aaaataacga ccatctacct gacgggccga ggcatcctta atcctgggga aagaaatagt
      961 atgcgggcct accttgtagc aagcttccag gtggttggta tgcctgagca ggcccagaac
     1021 ctcgaatttc caatcgtaaa gcccaaagag cgcacctatg cccacatcgt ctatgccggc
     1081 ctcctgcgca cggtccaggg ccgtgaggcg ataagcgtaa tcggccttat gcccccggtg
     1141 atacaaggaa taagcctgag gatcgtaggt ttcctgaaaa atctgataag tacctatacc
     1201 cgcagttttt accacacgaa aaccttctat gtccaaagga gcggcattga tattgacccg
     1261 cctgatctcg ccccggcctt tttttacgcc gtaagccaac ttcacggtat gggctatgta
     1321 atgcgcatcg taatcggggt gctcgccata gaccaaaatc aggcgctttt gtccctgatc
     1381 ttcgaggctt gctatttcct gaacgatttc ttcgtcgctc agggttttac gcacagcctc
     1441 gcgattggaa gccctgaaac cgcaatacac gcaatcgttt atacatttat tgccgatgta
     1501 cagaggagca aacaatacga ttcgattacc gtaaatttcg cgtttgagtt gccgggcacc
     1561 ctctttgatt tcttcgatca actcgggatc ttcggcgttt aacagcaagg cggtttcctg
     1621 catactgagg cgttcgcggg aaagcgagcg tgctatcgtg tcgcgtaccg cccgggcgtc
     1681 gggtctggca ttttgcaggc aagcacgtat ctcgtccgga tcgataaaag gctgcatcct
     1741 tcgatccgga atggcatatt tttggggatt aaacatcatt aggcatttgg ggtatcaaag
     1801 atacaactcc aaaaccgaaa acgacttttt tttatgaatt aaatctacag gctgcggcgg
     1861 taccagtcga gtattctgtc caaaccttcg gaaatatcca cccggtgttg ccagccgagt
     1921 ttatgcaact tgttcacatc gcaaagttta cgcatggtgc cgtcgggttt ggaagcatcg
     1981 aaacttatcc ggccggcata gcctgttttc tccgcaatca tggcagccag agcggctatg
     2041 ctgatttctt tgcccgtacc tatgttgatg tggcaattgc gcacttcgtc cgaagtatct
     2101 ttgggtcgca ggtcttcgaa atcgaccctt tccataataa agacacaggc atcggccatg
     2161 tcttcgctcc agaggaattc cctcaagggt tttcccgtgc cccaaagctc gaggcgatcg
     2221 gggtaaatgc cgtacttttg cagcaaaatc ttaatctcag gcaaaggagt cttgccgtcg
     2281 aagccttcta tcggacgact gtccagatcg cggcgcaaaa gggtttcatc gcccctgagc
     2341 aggcaagagg ctaaaaaaat cttgcgaacc atagccggca acacatggct gcgttccaga
     2401 tcaaaattat cgttagggcc gtagagattg gtaggcatca ccgcaataaa attggtgccg
     2461 tattgcaggt tgaaactttc acaaagctta agtccggcaa tcttggcaat ggcatagggt
     2521 tcgttggtgt attccagagg accggtcaac aaggcttcct cgcggatggg ctgggccgca
     2581 tcgcggggat atatgcaggt actgcccaaa aataacaatt ttttgacctt gtgacgatag
     2641 gctgctccta taatgttttg ctggatttgc aggttgcggt agatgaagtc ggccctgtac
     2701 acattgttgg cctgaatgcc gcccacatga gcggccgcca acaccacgta ttccggctgt
     2761 tcggctgcaa aaaaagcctc caccgcaggg ccttccatca aatcgagctc agcgtggctt
     2821 cgtcccagca agcgggtata gcctttggat aaaagattgt tccagatggc cgagcctacc
     2881 agtccccggt ggccggcaac gtagatctta ctgtgttttt ccatactatt ccggtcaatc
     2941 caactgcgat ttgagataga gtttttttac aaagcgcatg tcgtgcttta ccatcagctt
     3001 caccaggtct tcgaaagatg ttcgggtggg attccagccc aagagttttt ttgctttgga
     3061 gggatcgccc atcaattgtt ccacttcggc gggacggaaa tacttgggat cgacttcgac
     3121 cagcactttg ccggatttaa tgtcgatacc cttttcctct acaccctcgc cctcccaacg
     3181 caacgatatg tctgcttctt cgaaagccag cgtacaaaat tccctcacgg tatgcatctg
     3241 ccccgtggca ataacaaaat cctcgggcgt atgatgttgc agcatcagcc acatacactc
     3301 cacataatct ttggcataac cccaatccct gcgggccgat aaattaccca gatagagctt
     3361 gtcctgatgt ccctgggcaa tacgggcggc agccagcgtg atcttgcggg taacaaaagt
     3421 ctcccctcgc ctttcgctct cgtggttgaa caaaattccg ttcacggcaa acataccata
     3481 ggcttcgcgg taatttttgg taatccaaaa gccgtattgc tttgccaccc cgtaaggact
     3541 gcggggatag aaaggtgtgg tttctttttg cggcacttcc tgcaccaggc cgtaaagctc
     3601 cgaagtagaa gcctggtaaa tgcgggtgct tttttccaga cccagaatac gaacagcctc
     3661 cagtagacgc aaggtaccta cggcatcgga ttcgaccgtg tattccggca catcgaaact
     3721 gaccttgacg tgcgactggg ccgccagatt gtaaatttcg tccggtttaa cggtctgaat
     3781 tacccgaatg agcgaactcg aatcggtcat atcggcgtaa tgcaaattga tgagccgttt
     3841 gcgtttcatg tcgcgtaccc attcatcgag gtaaagatgt tcgatacgcc cggtattaaa
     3901 agaagaactg cggcgaatgg tgccgtgtac ttcgtaattc ttttcgagca ggaattcggc
     3961 cagaaaagac ccgtcctgac cggtaatacc ggtaatcaaa gctactttcg tcatagtgtc
     4021 tgagttaaaa tatttttggt tttaaaacga gttgtttttg aaaatcttag ttttaaaatc
     4081 gcttgggcaa aaccgccccg gcttactttt cagttttttg cggcaaaggc tcagcttgat
     4141 cttcgggcag caagggtttg gaagcgtcga tgcgtgtaaa cagcaaggcg ctgattacca
     4201 gcatgccggc gatcacaaac aaggggaaat tgtaattacc cgtgcccgag accagatagc
     4261 caaaaatggt ggcacaaaca aaaccgccga tgttgccggc tgtgttcatg gcgcccgata
     4321 tggctccggc gtattgctta ccaatgtcca aacaaatagc ccaggcactg ggcaacatca
     4381 ggtcgatcac cccgaaacag agcgaaagga aaataaagac ctgcatcttg cccggaataa
     4441 aggcagccaa aaacataaat accgccgaag ccgccaaacc gcctactccc agcaggcgac
     4501 ggcctttttt gagcccgtac tttttcgaaa aacgatcgct caggtagccg ccggtaatat
     4561 tgccggccac actcatcaaa aagggcacgg ctacggcgta ggtgagagca gcctttgaaa
     4621 agccccggcc ttcttccata aaagtgggga accacgaaaa gaaaaaccag ctgccccagg
     4681 cgtaaaagaa atacatggcg aggatcagcc agaattgttt actgtgcagt atagcccccc
     4741 aaggcacttt ttgtttagct acttccactt taggtttatc gtcgagctcg gccagttcgg
     4801 caggagtgat gcccttttta tcggcgggat tatcgcgaaa ccaccaatac caggcaatca
     4861 cccagaccac acccaaagct cccagaatat agaaggcctc gcgccagccc aggtgcacaa
     4921 tgacaggaat cacaataaag ggagtcaagg cccctcccac ccggctggcc gcccagatat
     4981 agccctgagc tctggcgttt tcggatttag gaaaccaacg ccctatggct ccggtcatac
     5041 aaggataagc tccggcctcc ccgataccaa aagcaaaacg gataaacagc agtgaaataa
     5101 agccgccggc catccccgtc aaaccggtaa acaccgacca ccaaagcaca ataacggcca
     5161 acaccttgcg ctgacctttg cggtcgccca acagacccag agggagttgc aaaagtccgt
     5221 aggatatgat aaaggcactc agcacccaac cccattgctt gtgagtcagc cccaggtcgg
     5281 ccataatact ggtgcccgcc accgaaatat tgatgcggtc gaggaaagta atcactccta
     5341 taaagaccag aaaaatcagc agaatattcc tttttttcat ggcttcttat ccgataagtt
     5401 ttttaagacc caacactcca aggagaggac tggataaaac ggttttgccg gtttcggctg
     5461 ccaatttttc ttccatgcgg gccatagatc cctgggccaa cacgattaca tctaccttgg
     5521 gggccaccgt tttcgaagct tccaaaataa gctcgtcgtg cttttgggga ttgccgccca
     5581 tggcagcctg aaaagctcct ttggccaagc cgtctaccaa ctcaatcttt ttgccctgtt
     5641 gggctgccaa acgttcgagc aagtcggctg tgggctgcaa agtggtgggc agagttgcca
     5701 gtacgccgat gcgggcagag aggcttatgg ctttgagggc catttgttcg tcgatacgga
     5761 tcaggggcac gggcataaaa aaccgagccg tatcggccac atcacccacc gaagagcagg
     5821 tattgaaaat atagtcggca ccggcatcca cggctgcgta atagcaggcc agcatacggc
     5881 gcttaacggc aggcgtcacc ctaccttcgg caatgacttc ctgaatcagg ctgtcgtcgg
     5941 caatgtg
//
LOCUS       DAOSGL010000109         5945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11785_length_5945_cov_3.772666, whole genome shotgun sequence.
ACCESSION   DAOSGL010000109 DAOSGL010000000
VERSION     DAOSGL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11785_length_5945_cov_3.772666"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1496
                     /locus_tag="PKY68_04590"
     CDS             141..1496
                     /locus_tag="PKY68_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOH24348.1"
                     /translation="MSAYSVCRLTAPDRVQGEIALPASKSISNRALVLYALAGKQSLR
                     TDFADCEDSLLMLNALQSPEAVKDAGAAGTVMRFLTAYYAQTPGEWTLTGTERMKQRP
                     IGLLVDALRQLGADITYTENPGYPPLLIKGKRLSGGRLSIDGSISSQYITALMLIGPY
                     VDGGLEIELLNKIASAPYIRMTLGLMQAFGAQLSFEGNLIRIAAGAYTAPEEFRVEAD
                     WSAASYWFEVLSLARSGQVLLHGLQADSLQGDAKVMEWFRDFGVQAEFIELPAGEKAS
                     ATGEVVTESVGSKVTGSPQAGKPDKGALLLSKTKQQCSFFEADFSQQPDLAQTFVVTA
                     ALRGIPFRIGGLESLKIKETDRLQALIREMLKLGFILEESSPNTLEWQGKKQPAQSRV
                     SIATYQDHRMAMSFAPVALRIKDQNEIKHFEIEDPTVVAKSYPGFWEDLKSCGFTIEY
                     K"
     gene            1493..1924
                     /locus_tag="PKY68_04595"
     CDS             1493..1924
                     /locus_tag="PKY68_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOH24349.1"
                     /translation="MTTFIWILFSALVLLGLFSWLLSKYFTGKAQSPADVTDPAEQDP
                     ETDDDVCCGQHLVCEKDSLMYQGEEIVYYDDDELDAYKGKAADAYTGAEIKDFEDVFY
                     TLQESDMAGWLRSLQLREINLPLQLRDEALMIVSERRQNIQ"
     gene            1967..4777
                     /locus_tag="PKY68_04600"
     CDS             1967..4777
                     /locus_tag="PKY68_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH24350.1"
                     /translation="MKAQFSHILLLSLLLVAVSSCSTQKNTWMARNYHALTTHYNIKF
                     NGRESYEAGLKQMELGHKEDYSSLLPVFPYNEQSASRATGNMNRCIEKCEKAIKTHSI
                     RVKPEKQPERNASEKEKLFYSQEEFNPVMGRVFLLMAKAQFYKADYLSAGSTCSYIIR
                     HFSTDKPLCDEAKIWLARSYYEQEWYYDAENIFYALNDQGFDPALNALYATSYADFLL
                     RQERIAQALPYLEIALKNTRKKQEKQRYLFLLAQMHQETGSPAKAYELFKAVPRKNPP
                     YEMALNARIRQTEVYPQGNSRSALKKLKRLSRNPNNQNYLDAIYYAMGNLYFSENDTL
                     KAIDSYNLAIAQSTSNGPYKAQALMTLGDYYYRNELFLEAAPCYQQAKGLIGKEHKET
                     ELVEERARVLDLLQPHLETIHLQDSLQWVADLPDEERDKLIEERITQAKKAARQEARL
                     RGQEDALSANRELTQANVREEETAPTVLPGMQNTDDSWYYYNPATLETGRREFQRNWG
                     RRRLEDNWRISNKGDFFSQNAFAGIADETTLPGAATDSLPGDALRGEGLLSDTEVGST
                     EDERTGPEFEASDDPTQEGYYLKDLPFTREQLQASMELIAEASFMSGMIFREQMGNND
                     LALRTFQNFEERFPEDTTYLPEIYYISYLMQMQSGNEQQADSLRSSLLSRFPQTQQAG
                     LLENPHFLDKLKEMYRLQDSLYASTYQHYLNDASDSVKLNCAYVETNYPKSELRPHFW
                     FLSALEAVKAGDAEEFLRLIRLIASEYPNTPLGPVSQQMIGYWEEGRRPVSFKGFFLA
                     SGMTIEDSLAMARLDSLATKFNYRPEEPHILALGYSPDSINENRLLFDVALYNFTNFL
                     IRDYELSLEKIGTQDVLMVRSFENQEDIYRYISTLSFQGEDPQEKYPGLKILIFSESN
                     LELLKEEFPEEVYRRFVETHYP"
     gene            complement(4933..5361)
                     /gene="tsaE"
                     /locus_tag="PKY68_04605"
     CDS             complement(4933..5361)
                     /gene="tsaE"
                     /locus_tag="PKY68_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOH24351.1"
                     /translation="MNMLPIEFQLSGIQEAARLLLNNFPDRRVFAFYGGMGAGKTTFI
                     QALCRRLGVKSSVNSPSFALINEYPLDKVDAVYHFDFYRINKLQEAYSIGSEDYFYSG
                     NYCFIEWPEKIEELLPEDCVKIELTVLETGVRRLSLKQGL"
     gene            complement(5358..>5945)
                     /locus_tag="PKY68_04610"
     CDS             complement(5358..>5945)
                     /locus_tag="PKY68_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOH24352.1"
                     /translation="YKFSYHKINDNSAGEKLLVNLQSYMDNPLNVVVFNFIDMLSHAR
                     TESRMIRELANDEAAYRSLTESWYKHSSMAGLLDYLSQQSCKVILTTDHGTIRVDNPV
                     KVVGDRNTNTNLRYKLGRTLEYNPRQVYEIKNPLQYKLPSPNLSTTYIFAQHNDYFVY
                     PNNYNYYASYYKHTFQHGGISLEEMVVPFILLDSK"
BASE COUNT         1608 a         1541 c         1454 g         1342 t
ORIGIN      
        1 agggcggcga tactgtgtta gtatacgcaa gccttgtgta aatcgtctcc gaaaaatcaa
       61 ttgagcccag cccgatccgt ttcgccggga aatccatcgc ttttttatac ctttgcagca
      121 aattgttttg ttccttcatc atgtccgctt attccgtctg ccggctcaca gctcccgatc
      181 gtgtacaggg agaaattgcc ttgcccgctt ccaaaagcat cagcaacagg gccctggtgc
      241 tctatgccct ggccggcaaa caaagcctga gaaccgattt tgccgactgc gaagacagcc
      301 tcctgatgtt gaacgctctg caaagccccg aagccgtcaa ggacgcggga gcggcaggta
      361 ccgttatgcg ttttttgaca gcctattacg cacaaactcc cggagaatgg acgcttacgg
      421 gaaccgaaag aatgaaacaa cgccccatag gtttactggt ggatgctctc cgtcaactgg
      481 gagcggacat cacctatacg gaaaatccgg gctaccctcc cctgctgatc aagggcaaac
      541 ggctcagcgg cggacgcctg tccatcgacg gaagcatcag cagccagtac atcacggctt
      601 tgatgctgat cgggccttac gtcgacggcg gcctcgaaat agagttactc aataaaatcg
      661 cctcagctcc ctatatccgc atgacacttg gtctgatgca ggcctttggg gcccaactga
      721 gcttcgaagg caatcttatt cgcatcgcag cgggtgctta caccgctccc gaagagtttc
      781 gggtggaagc cgactggtcg gccgcttcgt attggttcga agtgctgagc ctggcccgat
      841 cgggacaagt cctgctgcac ggcctgcaag ccgacagcct gcagggcgac gccaaggtga
      901 tggaatggtt ccgggacttt ggggttcagg ccgagtttat cgaacttccc gccggagaaa
      961 aggcctctgc gaccggggaa gtcgtgacgg aatcagttgg cagtaaagtc accggcagcc
     1021 cgcaggcagg aaaaccggac aaaggagccc tgctcctgag taagaccaag cagcaatgtt
     1081 ccttctttga agcagacttc agccagcaac ccgacctggc tcaaactttt gtagtaacgg
     1141 ccgccttaag ggggattcct tttcgcatcg gcggactgga aagcctgaaa ataaaagaga
     1201 ccgaccgctt gcaggccctg attcgggaaa tgctgaaact gggctttata ctcgaagaga
     1261 gcagccccaa tacgctggaa tggcagggca aaaaacagcc cgcacagagc cgcgtcagca
     1321 tagccaccta ccaagaccac cgtatggcca tgtcgtttgc tcccgtggca cttcgaataa
     1381 aagaccaaaa tgaaataaaa catttcgaaa tagaagatcc gaccgtggtc gccaaatctt
     1441 accccggatt ttgggaagat ctcaaaagct gtggttttac tatagaatac aaatgacaac
     1501 gtttatctgg atattattta gcgctctggt gcttctgggt ttattcagtt ggctgctgag
     1561 taaatatttc accggcaaag ctcagtcgcc cgcggacgta acagacccgg cggaacaaga
     1621 cccggaaacg gacgacgacg tctgctgcgg acaacacctc gtgtgcgaaa aagacagcct
     1681 gatgtaccag ggcgaagaaa ttgtctatta cgacgacgac gagttggatg cttacaaggg
     1741 aaaagcagca gatgcctata ccggagcaga gatcaaagac ttcgaagatg tgttttacac
     1801 cttgcaggaa agcgatatgg ccggctggtt gcgcagcctg cagttgagag aaataaacct
     1861 gcctttgcag ttgagagacg aggccctgat gatcgtaagc gaaagaagac aaaacataca
     1921 ataaaaccgg caaaaagctg tttaataaac gatcggttct taagtattga aagcacaatt
     1981 ttcacatatc cttttgctaa gcctgctgct ggtggcagtc tcctcttgtt ccacccagaa
     2041 aaacacctgg atggcccgga attaccacgc cttgaccact cattacaaca tcaaattcaa
     2101 cgggcgcgaa agttacgagg cggggctgaa acaaatggag cttggtcaca aagaggatta
     2161 ttcctcgctg ctgcctgtgt ttccttacaa cgagcaatcg gcttcgcggg cgacaggcaa
     2221 tatgaatcgc tgcatagaaa aatgtgaaaa ggccatcaaa acccattcca tacgcgtaaa
     2281 acccgaaaaa caaccggaac gcaacgccag cgagaaagaa aagctttttt acagtcagga
     2341 agaattcaac cctgtcatgg ggcgggtatt tttactgatg gccaaggccc agttttacaa
     2401 agccgattac ttgtcggccg gttctacctg ttcctatatc atccggcatt tttccaccga
     2461 taaaccgctc tgcgacgaag ccaaaatctg gctggccaga tcgtattacg aacaggaatg
     2521 gtattacgat gcggaaaata ttttttacgc actcaacgat cagggtttcg atcccgcgct
     2581 caacgccttg tatgccactt cttacgccga ttttctactg aggcaggaaa gaatcgccca
     2641 agccctgccc tatttggaaa ttgccctgaa gaacacccgc aaaaaacagg aaaagcaacg
     2701 ctatcttttt ttgttggctc aaatgcatca ggaaacaggc tctccggcca aagcctatga
     2761 actcttcaaa gcggtgccgc gcaaaaatcc gccttacgaa atggccctga atgccaggat
     2821 aaggcaaacg gaagtctatc cgcaaggcaa cagccgatcg gctctgaaaa aattaaaaag
     2881 gctcagccgc aatcccaata accaaaacta tctggatgcc atctattacg ctatgggcaa
     2941 cctgtacttt tcggagaacg atacgctgaa agccatcgac agctacaacc ttgccattgc
     3001 tcaaagcact tccaacggac cttacaaggc ccaggccctg atgactttgg gcgattatta
     3061 ttatcgtaac gaactctttc tcgaagcagc tccctgttat caacaagcca aaggcctgat
     3121 cggaaaggag cacaaagaga cggaactggt cgaagaaaga gcccgggtac tggatctgct
     3181 tcagccccac ctggaaacca tacatctgca ggacagtctg caatgggtgg ccgacctacc
     3241 cgatgaggaa cgcgataaac tgatagaaga acggattacc caggccaaaa aggcagcccg
     3301 ccaggaggcc aggcttcgcg gtcaggaaga cgctctgagt gccaaccggg agctgaccca
     3361 ggccaatgtg cgtgaagaag aaacagcccc caccgtcttg ccaggcatgc aaaacaccga
     3421 cgacagctgg tactattaca atccagccac tctggagacc gggcggcggg agtttcaacg
     3481 caactggggc agacgcagac tcgaagacaa ttggcgtata agcaacaagg gcgacttttt
     3541 cagccaaaat gcttttgccg gaatcgctga cgaaaccact ttgcccgggg ccgccaccga
     3601 cagtctgccg ggtgatgctc tgagaggcga aggtctgctt tccgacacgg aagtcggcag
     3661 caccgaagac gaaagaaccg ggccggaatt tgaggcttcc gacgatccca cccaggaagg
     3721 ctattacctg aaagacctgc cctttacccg ggaacaacta caggcatcga tggaactgat
     3781 agccgaagcc agttttatgt cgggcatgat tttcagagaa caaatgggca acaacgatct
     3841 ggctttgaga actttccaaa attttgaaga acgttttccc gaagacacta cttatctgcc
     3901 cgagatttat tacatctctt acctgatgca gatgcagagc ggaaacgaac agcaagccga
     3961 cagcctgaga agcagcttgt tgagcagatt ccctcaaact cagcaagccg gcctgctcga
     4021 aaatccccat tttttggata aactcaaaga aatgtaccgc ttgcaggatt ccttgtacgc
     4081 aagcacctat cagcactacc tgaacgatgc ttccgatagc gttaagctca attgtgccta
     4141 cgtggaaacg aattacccca aaagcgaact ccgaccccat ttttggtttt taagcgctct
     4201 ggaggccgtt aaagcaggag acgccgaaga atttttacgt ttaatccggc tgatagcctc
     4261 ggaatacccc aacactccct tggggcccgt aagtcaacaa atgataggct attgggaaga
     4321 aggccgccgg ccggtgagct tcaaaggctt ctttttggct tcgggcatga ctatcgaaga
     4381 ttcgctggct atggcccgtc tggattcgct ggccaccaag ttcaactacc ggcccgaaga
     4441 gccccatata ctggccttgg gttactctcc cgattccatc aacgaaaacc gcctgctttt
     4501 tgatgtggcg ctctacaatt tcaccaattt tctgatcagg gattatgagt tgagcctaga
     4561 aaaaatcggt actcaggatg tcctgatggt gcgcagtttc gaaaatcagg aagacatcta
     4621 ccgctatata tcgactttaa gctttcaagg cgaagaccct caggaaaaat accccgggct
     4681 taaaatcctg atcttttcgg aatccaatct ggaactgctg aaggaggaat ttcccgaaga
     4741 ggtttaccgt cgctttgttg aaactcacta tccttaggaa ccccactatc ctgagcgatc
     4801 ctacaagtac gagcattccg catacctcag gccatcctgc agcccggcca ttctattagc
     4861 ctgaacaatc ctataagcct cagccatccc acaagtccgg gcaagcctac aaccctacag
     4921 gcccgaacct ttctacaagc cctgcttaag gctgagacga cgcactcccg tctcgagcac
     4981 ggtcagctcg attttaacgc agtcttcggg cagtagttct tctatttttt cgggccattc
     5041 gataaaacag taatttccgc tgtagaaata gtcttcgctg cctatgctgt aagcttcctg
     5101 tagtttgttg atgcgataaa aatcaaaatg atagaccgca tcgaccttgt cgagggggta
     5161 ttcgttgatc agggcaaaac tgggactgtt tacggagctt ttcacgccca gccggcggca
     5221 gagtgcctgg ataaatgttg ttttgccggc tcccatgccg ccgtaaaaag cgaagaccct
     5281 gcgatccgga aaattgttca gcaacaggcg agctgcctcc tgtataccgg ataattgaaa
     5341 ttcgatgggc aacatattta tttggaatcc aacaaaataa acggaactac catttcttcg
     5401 agcgaaatac cgccgtgctg aaaagtatgc ttgtagtacg aagcatagta attgtagttg
     5461 ttggggtaga caaaataatc gttgtgttgg gcaaaaatat aggtggtgct caggttgggg
     5521 ctaggcagtt tgtattgcag ggggtttttg atttcgtata cctgccgggg attgtattcg
     5581 agggttctgc ccaatttata acgcaagtta gtattggtgt tgcgatcgcc caccaccttg
     5641 accggattat ccactctgat ggtgccgtgg tcggtggtca gtatcacttt gcaactttgt
     5701 tgcgaaagat aatcgagcaa tccggccatg gaagaatgct tgtaccagga ttcggtaagt
     5761 gaacggtaag ccgcttcgtc gttggccagt tcccttatca tcctcgattc tgtacgagcg
     5821 tgcgacaaca tatctataaa attgaacacc accacattaa gcggattatc catatagctc
     5881 tgcaagttga ccaacaattt ttcgccggcc gaattatcgt tgattttatg gtagctgaac
     5941 ttata
//
LOCUS       DAOSGL010000110         5896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11912_length_5896_cov_4.025509, whole genome shotgun sequence.
ACCESSION   DAOSGL010000110 DAOSGL010000000
VERSION     DAOSGL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11912_length_5896_cov_4.025509"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1229
                     /gene="pdxB"
                     /locus_tag="PKY68_04615"
     CDS             90..1229
                     /gene="pdxB"
                     /locus_tag="PKY68_04615"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HOH24353.1"
                     /translation="MKIVCDHKIPFLKGALEPYAEVVYLPGNRIAAADVKDADALITR
                     TRTKCNAALLEHSAVKMIATATIGYDHIDTEWCEQKGIAWTNAPGCNSGSVQQYIASV
                     LAGLAQEPGFRFEDKTLGVVGVGNVGKKVARLAEILGFRVLLNDPPRARAEGPEAFVS
                     LEQLIRESDIITLHVPLETKGPDATYHLFDAKRLQGLSPRQILINSSRGEVVDNQALK
                     TCLKQVGIKAAVLDVWEKEPAVDLELLDLVLYGTPHIAGYSADGKGNGTTMSVRAIAK
                     KFNLPLQDWQAVHLPLPAQSIYPELNGRGKSPQEVLCKAILHTYRVEDESRVLKQNPG
                     TFEQQRADYAVRREFGCYTLRLRGVEKPVAEKLAALGFKLEILPE"
     gene            1265..2716
                     /gene="gnd"
                     /locus_tag="PKY68_04620"
     CDS             1265..2716
                     /gene="gnd"
                     /locus_tag="PKY68_04620"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOH24354.1"
                     /translation="MKQLSDIGLVGLAVMGENLVLNMESKGFTVSVFNRTVAKVDNFV
                     NGRGKGKNIIGTRSIEELVASIKRPRKVMLMVKAGKAVDDFIELLLPHLEPGDIIIDG
                     GNTHYPDTNRRTAYLESKGFLYIGTGVSGGEEGALTGPSIMPGGSKAAWPHVKPIFQA
                     IAAKVEDGPCCDWVGENGAGHFVKMVHNGIEYGDMQLICEAYHIMKDMLGMNTDEMHE
                     VFAEWNKGDLDSYLIEITRDILAYKDENNQPVVEYILDTAGQKGTGKWTGIAALEHGV
                     PLTLIGEAVFSRCLSAQKDERVAASKILQGPKPAFKGDKKAFIEDLRKALFASKVVSY
                     AQGYTLMRSAAEEYGWDLNYGGIALMWRGGCIIRSVFLGKIKEAFDKDHNIPNILIAP
                     YFTEKLKEAQNSWRNVVAQTALNGIPAPALSSALAYYDGYRCERLPANLLQAQRDYFG
                     AHTYERIDKPRGEFFHTNWTGRGGDTVATTYIA"
     gene            2910..3803
                     /locus_tag="PKY68_04625"
     CDS             2910..3803
                     /locus_tag="PKY68_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOH24355.1"
                     /translation="MKQQINSFALPFAMLTGGIFHTFFNQLNFLTPYLIFFMLFFPYC
                     RIQLKDMRLRSFHLWLILYQVAVSLLAYLLLSLFSEELGQGVLICILAPTATAAIVVG
                     GMLGANIALMASYTLFCNLVVAIMAPILFSFVGIQAELYFWTSFWLIFKRVFPLLVLP
                     FVAALLLRKFAPGFNSKIQKMSSVSFYLWIFALAIVSGRIVHFMLDQQKSDYRMAAVL
                     AAAALIICVFQFLFGRFIGRRYGETVSGGQSLGQKNTILAIWMAQTYLNPIAAIAPAS
                     YVLWQNLVNSYQLWRKNRKTS"
     gene            3816..4700
                     /locus_tag="PKY68_04630"
     CDS             3816..4700
                     /locus_tag="PKY68_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HOH24356.1"
                     /translation="MKKNIKIALIQTAVSADPEQNKSMLAQKIADCRRRGAELVVLQE
                     LHNTLYFCQEESTDLFDLAESIPGPSTAFYSKLAAQHRLVLVCSLFERRAAGLYHNTA
                     VVFERDGSFAGTYRKMHIPDDPGFYEKFYFTPGDLGFKPIETSLGKLGVLICWDQWFP
                     EAARLMTLKGADLLIYPTAIGWDLRDDEQEKARQLDAWITVQRGHAVANGLPVVAVNR
                     SGYEQSRADSQQGILFWGNSFVAGPQGEIQARAGEKDEILLTDINLDRNEEVRRWWPF
                     LRDRRIDAYQNLLKRFVD"
     gene            complement(4747..5832)
                     /locus_tag="PKY68_04635"
     CDS             complement(4747..5832)
                     /locus_tag="PKY68_04635"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOH24357.1"
                     /translation="MHDFVRRIEHLLTEHDCVIVPGLGGFVQNETEAHFDYKDQLFYP
                     PSKQIGFNARLHFNDGLLAQSYQQDYNIDFEEARDRIRDRVQELQGYLSQGKYLTLGR
                     IGYMHRSEEGQIVFRPGKNNPFVPSAYGLRPLYCNVIAQKAAPERTHDLGRWLVAVAA
                     CLLLMLLLNPVQHRLNNKNPVLQEASVMSACSLLNEPAANIPVKDSATPASSVTKLTE
                     AGEIVEAGEIVKTVEIAETARFAETAGIAEVAGLAETARIAQVADAVGVAEVSKASEP
                     AKVFPSPFTPKYCIVVASFTNEADAQRWLEQKQLKKEYPQAGLVSKEGRTRVYVLGFD
                     QREAALSYLAKFRAGNQKYAKSWVLVN"
BASE COUNT         1418 a         1484 c         1508 g         1486 t
ORIGIN      
        1 caatattatg cccgacggag aagaaatttt ctatatctcc aatccggctt tgggtttgtg
       61 ggcacataag gacagattca actaagggga tgaagatagt atgcgatcat aagattcctt
      121 tcctcaaagg tgcattggaa ccttatgctg aagtcgttta cctgcccggc aaccggattg
      181 cggctgcaga tgtaaaagac gcagacgccc tgattacccg tacgcgaacc aaatgcaatg
      241 ccgccttgct ggagcattct gcggttaaaa tgatcgctac cgccaccatc ggttacgatc
      301 atatcgatac cgaatggtgt gaacaaaagg gcatagcctg gaccaatgcc ccgggttgta
      361 actcagggtc cgtgcaacag tatatcgctt cggtactggc cggtctggca caagagccgg
      421 gcttccgttt cgaagataaa accctgggcg tggtgggtgt tggcaatgtg ggtaaaaaag
      481 tggcgcgtct ggccgaaatc ctgggcttca gggtgctgtt gaacgacccg ccccgtgccc
      541 gcgccgaagg ccccgaggct tttgtttcgc tcgaacagct tatccgggag agcgacatca
      601 tcactctgca tgtgcccctc gaaacaaaag gcccggatgc cacttatcac ctgttcgacg
      661 ccaaacgcct gcagggcttg tcgccgcgtc agatactcat caattcctcg agaggagaag
      721 tggtcgacaa ccaggccctg aaaacctgcc tgaagcaagt cggaataaaa gcggcggtgc
      781 tcgatgtctg ggaaaaggag ccggccgtcg atctggaact gctggacctg gtgctttacg
      841 gaacacccca tatagcgggt tattccgccg acggcaaagg caacggcacc accatgagtg
      901 taagggccat cgccaaaaag ttcaatctgc cgctccaaga ctggcaggcc gtccacttgc
      961 ccctgcccgc gcaaagcatc tatcccgagc ttaatggcag aggcaagagc ccgcaggaag
     1021 tgttgtgcaa ggctattttg catacctaca gggtggaaga cgaaagtcgt gtgctaaaac
     1081 aaaaccccgg caccttcgaa caacaacggg ccgactacgc agttcgcaga gaattcggct
     1141 gctataccct tcgcttgcga ggagtggaaa agcctgtcgc agagaagctt gctgccttag
     1201 gtttcaaatt ggaaatcctt cctgaataaa aacaaattat aataactatt taaataacac
     1261 gaacatgaaa caactatcag acattggatt agtaggactg gccgttatgg gcgagaacct
     1321 cgtattgaac atggaaagca aaggctttac cgtcagcgta ttcaaccgca ctgtggccaa
     1381 agtagataat tttgtcaacg gacgcggcaa gggtaaaaac atcatcggca cccggagcat
     1441 cgaagaattg gtggcttcga tcaaacgccc ccgcaaagta atgctcatgg ttaaggcagg
     1501 caaggcagta gacgatttta tcgaattgct gcttccccat ttggagcccg gcgatattat
     1561 tatcgacgga ggtaacaccc attaccccga taccaaccgt aggacagcct acttggaaag
     1621 caaaggattt ttgtacatcg gcaccggtgt ttcgggcgga gaagaaggcg ctcttaccgg
     1681 tccttccatc atgcccggcg gatcgaaggc tgcctggccc catgttaaac cgattttcca
     1741 ggctattgcc gccaaggtcg aagacggccc ctgctgcgac tgggttggtg aaaacggagc
     1801 cggtcatttt gtcaagatgg tacacaacgg catcgaatac ggcgatatgc agcttatctg
     1861 cgaagcctat catatcatga aagacatgct gggcatgaat accgacgaaa tgcacgaagt
     1921 ctttgccgaa tggaacaagg gcgatctgga tagctacctg atcgaaatca cccgggatat
     1981 tctggcttac aaggacgaaa acaatcaacc ggttgtggaa tacatcctcg ataccgccgg
     2041 ccaaaaaggc accggcaaat ggaccggcat tgccgccctc gagcacggtg tgcctctcac
     2101 cttgatcggc gaagctgttt tctcccgttg tttgtctgct caaaaggacg aacgcgttgc
     2161 tgcttccaaa attttgcaag gccccaaacc tgctttcaag ggcgataaaa aagcctttat
     2221 tgaggacttg cgcaaagctt tgtttgcctc caaggtggtt tcgtatgccc agggttacac
     2281 cctgatgcgt tcggctgccg aagaatacgg atgggatctg aattacggcg gtattgccct
     2341 gatgtggcga ggcggctgta ttatccgctc ggtcttcctg ggcaaaatca aagaagcctt
     2401 cgacaaagac cacaatattc ccaatatcct gattgctccc tattttaccg aaaaattgaa
     2461 agaagctcaa aacagttggc gtaacgttgt agcccaaacg gctctgaacg gtatccccgc
     2521 tcccgccctg agttctgctc tggcttatta cgacggttat cgctgcgagc gccttccggc
     2581 caatctcttg caggcacaac gcgactattt cggcgcacac acctacgaac gcatcgacaa
     2641 gccccgcggt gaatttttcc acaccaactg gaccggtcgt ggcggcgata cggtagctac
     2701 cacttacatc gcttaacttt tccaaacgat agccgaatta atcggcaatt gttttaacat
     2761 tttatgagaa tcccaaaagt ctgaatggaa cttttgggat tctttttttg ggcttacttc
     2821 taacattttg ttttgggagc tttacttact gttttgcttt tgtcgtcgtt tttttggtaa
     2881 ttttgccgcc tgatgtccga tttgcctcta tgaaacaaca aataaacagc tttgccctgc
     2941 cgttcgccat gttgacggga ggtatctttc ataccttttt taatcagttg aatttcctca
     3001 ccccttacct gatttttttc atgctctttt tcccttattg ccgcattcag ctcaaagata
     3061 tgcgcctgag gagttttcac ctttggctca ttctgtacca ggtggccgtc agtctgctgg
     3121 cctatctgct attgagtctg ttcagtgaag agcttggcca gggggtttta atctgcatac
     3181 tggctcccac ggctaccgcg gcgatcgtgg taggtggcat gctgggcgcc aatattgccc
     3241 tgatggccag ctataccttg ttttgcaatc tggtagtggc gattatggct cccattctgt
     3301 tctcttttgt gggcatacag gcggaattgt atttctggac ttcgttttgg ctgattttta
     3361 aaagggtctt ccccttgttg gtgctcccct ttgtggcagc tttgcttttg cgaaaattcg
     3421 ctcccggctt taatagtaaa attcaaaaaa tgagttctgt ttcgttttat ttgtggatat
     3481 ttgccctggc gattgtgagc ggacgcattg tgcactttat gctcgaccag caaaaatcgg
     3541 attacaggat ggctgccgtg ctggcagcgg cggctttgat aatttgtgtg tttcagtttc
     3601 tgttcggacg ctttataggc cggcgttacg gcgaaaccgt cagcggaggt cagtcgctgg
     3661 gacaaaaaaa caccatcctg gccatctgga tggctcagac ttacctgaat cctattgcag
     3721 ctatcgctcc tgcctcttac gtgttgtggc agaatttagt gaacagctat caattgtggc
     3781 gtaaaaacag aaaaacgtct taattttttg gctctatgaa gaagaacatc aagattgccc
     3841 tgattcaaac cgccgtttcg gccgatccgg aacaaaacaa atctatgctg gcgcaaaaaa
     3901 tcgccgattg ccggcgacga ggtgcggaat tggtggtttt gcaagaattg cataatactc
     3961 tgtatttctg ccaggaggaa tccaccgacc tgtttgacct ggccgaaagc attcccggtc
     4021 cctctaccgc gttttactcg aagttggcgg cacagcaccg actggtgctg gtttgttctt
     4081 tgttcgagcg cagggcggcg ggcttgtacc acaatacggc ggtggtattc gaacgggacg
     4141 gttcttttgc cggcacctac cgaaagatgc atatccccga tgatccgggc ttttacgaaa
     4201 agttttattt tactcccggc gatctgggct tcaagcctat agaaacttcg ctggggaaat
     4261 tgggcgtcct gatctgctgg gatcagtggt ttcccgaagc ggcacgtctt atgaccctga
     4321 agggagctga tttgcttatt taccccacag ccatcggctg ggatttgcgc gacgacgagc
     4381 aggaaaaagc gcgtcagctc gatgcctgga taacggttca acggggacat gccgtggcca
     4441 acggcctgcc ggtggtcgct gtaaacaggt cgggttatga acaaagccgg gccgattccc
     4501 aacaaggcat cttgttttgg ggcaacagct ttgttgccgg gcctcagggg gaaatacagg
     4561 ccagggccgg cgaaaaagat gaaattctcc tgacggatat caacctggat cgcaacgaag
     4621 aggtacgacg ctggtggccc ttcttgcgcg accgccgcat agatgcctat caaaatttgc
     4681 tgaaacgttt tgtggattaa atcttctgaa gttattgacc aaaaaacgct ttgaccaaaa
     4741 accgatttaa ttgaccagca cccaggattt ggcgtacttt tgattaccgg ccctgaattt
     4801 agctaaataa ctcaaagctg cctcgcgttg gtcaaaaccc agaacataga cccgtgttct
     4861 gccctccttg ctgaccagtc cggcctgagg gtattccttt ttgagttgtt tctgttccag
     4921 ccagcgttgg gcatcggctt cgttggtgaa gctggctacc actatacagt atttgggagt
     4981 gaatggagac gggaaaactt ttgccggttc ggacgctttg gaaacttcgg caacacctac
     5041 agcatcagca acttgggcaa tccgggcagt ttcggcaagc ccggcaactt cagcaatccc
     5101 ggcagtttct gcgaatctgg cggtttcggc tatctcgaca gttttaacaa tctcaccggc
     5161 ttcaacaatc tcaccggctt cggtaagctt ggtgacagag cttgccggtg tggctgagtc
     5221 ttttaccggg atgttggcgg ctggctcatt gaggaggctg caggcggaca tgaccgaggc
     5281 ctcctgcaag accgggttct tgttgttcag ccgatgttgc acagggttca acagcaacat
     5341 caacaacaaa caagcggcca ctgccaccaa ccagcgaccc aaatcgtgcg ttctttccgg
     5401 agcagccttt tgcgcaatta cattgcagta caaaggcctc aggccatagg ccgaagggac
     5461 aaagggattg tttttaccgg gacgaaagac aatctgccct tcttcgctgc ggtgcatgta
     5521 gccgattcgc cccagggtaa ggtatttacc ctgagataga tagccttgaa gctcctgcac
     5581 cctgtccctg attctgtccc tggcctcctc gaagtcgatg ttgtaatcct gctggtaaga
     5641 ttgagccagc agaccgtcgt tgaaatgcaa acgggcattg aatccgattt gtttggaagg
     5701 aggataaaaa agttgatctt tgtaatcgaa atgcgcctcc gtttcgtttt gcacaaaacc
     5761 acccaggccc ggaacgataa cacaatcgtg ttccgtcagc aagtgttcga tacgtcgtac
     5821 aaaatcgtgc atagagccgg gtgaggtatt ttcggatgtg atgcagacaa aattacacag
     5881 gatattcaag ttttac
//
LOCUS       DAOSGL010000111         5886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_11945_length_5886_cov_4.106328, whole genome shotgun sequence.
ACCESSION   DAOSGL010000111 DAOSGL010000000
VERSION     DAOSGL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11945_length_5886_cov_4.106328"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKY68_04640"
     CDS             complement(<1..306)
                     /locus_tag="PKY68_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH24358.1"
                     /translation="MSKLSLIIQREYTSRVKKKSFLIMTFVMPLALVLIILIPLLLSS
                     VKDNKVRKIAVIDAAGLYLPRLQNTPNYQFIPCGDSTEACKNKADYYAYLIIEGNLTE
                     "
     gene            complement(303..1226)
                     /locus_tag="PKY68_04645"
     CDS             complement(303..1226)
                     /locus_tag="PKY68_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH24359.1"
                     /translation="MYYVETRSIVKQYTGHLALDKVSIQVPEAKIYGLLGPNGAGKTT
                     LIRILNQITYPDSGEVLLNGNPLKENDVYHIGYLPEERGLYKKMKVAEQALYLARLKG
                     LSKQEAKKRLRYWFDKFNIRDWADKKLEELSKGMQQKVQFIITVLHEPKLLIFDEPFS
                     GFDPVNTQLIKEEILNLKAQGSTILFSTHNMASVEEICDEIGLINKSKLILQGKVQDI
                     KLSHRSGIYLVRLRLPEDPDRAKSQLEKSPFFKIESSSLDPYHWQLELSIRDGLNNNS
                     VFRELYEHFEIVSFEEKIPTMHEIFIKSVKS"
     gene            complement(1258..3252)
                     /gene="dnaG"
                     /locus_tag="PKY68_04650"
     CDS             complement(1258..3252)
                     /gene="dnaG"
                     /locus_tag="PKY68_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOH24360.1"
                     /translation="MIDQITINRILDAARIEEVVGEFVHLRKRGVNYLGLCPFHDDKT
                     PSFSVSPAKGICKCFSCGKGGNVVHFIMEHEQLSYYEALKWLAKKYHIEVVEKQLSPE
                     ELQSKNERESMFVLNEFARDFFKSCLKKAGEGMSIGLKYFLERGLREDTIEKFQLGYA
                     PDERDAFTKNALAKGYQKDFLLKTGLSVADEQGQLFDRYRGRVIFPIHGISGKIRAFG
                     GRILKKDAKAAKYINSPESEIYSKSAELYGIYFARQAIVKQDRCYLVEGYTDVLSLHQ
                     SGVENVVASSGTALTKGQIQLIHRFTDNITVLYDGDAAGIKASLRGIDLLLEEGLNVK
                     IVLLPEGEDPDSFARGHSASALLDYLRKSETDFIRFKTEVLLRDAGDDPVRRARLIED
                     IVESISLIPNNIIRSEYVKECSRLLDSPELLIQKEVDKKRRKYIERLRQQRGLRESPG
                     TEPAYTPAEPLQQPIPAQADTQKKGSTPFSPYDEHERNLMRMIVRYGEQQINLDKNLY
                     PENFRPTVLEFISMDLENEEIEFQHPVYKLMYKEALAHHKEPHFVAERFFLNHHRAEI
                     SLMAGELISEEYELSKIHSRFQVVVEEKDRLHYLVPQFLEELKWKIVKEELAELRSKL
                     KIAEQNKDESTLRDLLEEIKILQELDKELARSLGERIITP"
     gene            3603..>5886
                     /locus_tag="PKY68_04655"
     CDS             3603..>5886
                     /locus_tag="PKY68_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24361.1"
                     /translation="MFKNTHRAWLFLLSLLLVVGACSDPYPYDDKEPDWLGSSIYDYL
                     VEKGEYTYFTRIIDTCNYREVLAKTGSKTMFVCKDSAFEEYFRKNTDGISRFEDFSVE
                     QLNQIMEYAMVNDASLIETLSYAVGYVKGQVMRRATSLDPFDMLSFEQGDEIPKNKYF
                     EPYREKGLYILNDDTSPTLIHFFEAQMKEKGITDEDFSILFNGKTRQTNDAHLFGIKV
                     IERDITCKNGYIHVLESLMLPRDNMAGYIRKSDSLSIFASLMDRFSAPYYTSSGTELY
                     KTLHPEFRDSIFVRNYFQKVSGTSGAYSILDPNGELIPSDELLRYDPGWNSYQYTSLS
                     MDMGAMFVPDDDAMKFYFSPEGNGSFLHERYKVWDSVPNDIVAMFINAHMKYSFISSV
                     PSKFQGLKDEDGFEIGIEKDNILRARVLCNGAVYVTNEVFPPVDYASVMAPVLVNERT
                     QIFNFALRNLDFNIYLRSLESFYSFLVPFDESFTTYLYPAGQGHETKKMIKLKFNTTS
                     EMVDAVTYTYDDATGQLKDSINVVSSAIALDCLNDIMDYHIVVGDIESGQEYYQTKGK
                     GEIRVQGSGIGMKVWGGGNMEMNRRWGSSWDDNKYTGEVTDILNLKNGKTYFVSNLLQ
                     QPITSVYKTLSNQPEFSKFLSAMMGVPDDMKEHLMLTKDPRTPGLDFNINFFSAFHYT
                     LYAPNNAAMDRAYAAGLPTWDQIAAVPTADDEGAKRAAMIQKVARFLKYHFQDNSVYI
                     GGELLSDVKYETATKNNITNK"
BASE COUNT         1490 a         1291 c         1350 g         1755 t
ORIGIN      
        1 ttccgtcaaa ttcccttcta taatgaggta agcgtaataa tcggctttgt ttttgcaggc
       61 ttcggtgctg tcgccgcaag gaataaattg ataattcggc gtattctgca agcggggaag
      121 atagagccca gcggcgtcaa tgaccgcgat ttttctgact ttattgtctt tgaccgaaga
      181 caataaaagc ggtataagta taatcaatac aagggccaag ggcatgacaa aggtcattat
      241 cagaaaagat tttttcttta cccgggaggt gtattcccgt tggataatca gcgaaagttt
      301 gctcatgatt ttacgctttt aataaagatc tcgtgcatgg tgggaatttt ttcctcaaaa
      361 gaaacaatct cgaagtgctc gtagagttct ctgaaaacac tgttgttgtt taatccgtct
      421 ctgatactca attcaagttg ccagtggtat gggtcgagcg agctgctttc gattttgaag
      481 aaaggagatt tctccagttg gcttttggcc ctgtcggggt cttcgggcag cctgagcctt
      541 accagataaa tgccgctgcg gtggctaagc ttgatgtcct gcactttgcc ttgcagtatc
      601 agtttggatt tgttgatcaa accgatttcg tcgcagattt cttcgaccga ggccatgttg
      661 tgcgtcgaaa aaagtatggt ggatccctgg gcttttaagt ttaaaatttc ttctttaatc
      721 agctgtgtat tcaccggatc gaagccgctg aagggctcat cgaaaatcag gagtttgggt
      781 tcgtgcagaa cggtgatgat gaattgaact ttttgctgca tgcctttgga aagctcctcg
      841 agtttcttat cggcccagtc gcggatattg aatttgtcga accagtacct gagtcttttt
      901 ttagcctcct gttttgacaa gcctttgagt ctggccaggt aaagagcctg ctcggctact
      961 ttcatctttt tgtacaggcc cctttcttcg ggtaagtagc caatgtggta aacatcgttc
     1021 tcttttagag ggtttccgtt cagcaaaact tctccgctgt cgggataggt aatctggtta
     1081 agaatgcgga taagggtggt ttttccggcg ccgttggggc ctaacaggcc gtagatttta
     1141 gcctcgggca cctgtatact cactttgtcc aaagccaaat gtccggtgta ttgcttaaca
     1201 atgcttcggg tttcaacata atacatacag tgctgtagtt aatttgaatg tgtaaaatca
     1261 gggtgtaatt attctttccc ccaagcttct ggccagctct ttgtccaact cctggagtat
     1321 ctttatttct tcgagcaagt cgcgcagggt cgattcatct ttgttttgtt ccgctatttt
     1381 aagtttagat ctgagttcgg ccagttcttc tttgacaatc ttccatttca gttcctcgag
     1441 aaattgggga accaggtaat gcaatctgtc tttttcttct acgaccactt ggaaccggct
     1501 gtgaattttg ctcagttcgt attcttcaga gatcaattct cccgccatca ggctgatctc
     1561 ggcccggtga tggtttagaa aaaagcgttc ggctacaaaa tgaggttctt tgtggtgagc
     1621 cagggcttct ttgtacatca atttgtaaac cggatgttga aattcgatct cttcgttttc
     1681 gagatccatg gaaatgaatt cgagcaccgt gggccgaaag ttttcggggt agaggttttt
     1741 gtcgaggttt atttgctgtt cgccgtagcg gactatcatg cgcatcaaat tgcgttcgtg
     1801 ttcatcgtaa gggctgaatg gtgtacttcc ttttttttga gtatccgcct gtgcggggat
     1861 tggctgttgc aacggctcgg caggcgtata agcgggctca gttccggggc tttctctcaa
     1921 tcctctctgt tgacgaaggc gttcgatgta tttccggcgt tttttgtcga cttctttttg
     1981 aatcagcaat tcgggagaat cgagcagccg gctgcattct ttgacgtatt cggatctaat
     2041 gatattgttg ggaatcaggg agatgctttc gacaatatct tcaatgagcc ttgctcttct
     2101 caccgggtcg tccccggcgt cgcgcaggag cacttcggtt ttgaagcgaa taaagtcggt
     2161 ttcgcttttc ctgaggtaat cgagcaatgc cgacgcgctg tgtccccggg caaaagaatc
     2221 ggggtcttca ccctcgggca gtaaaactat tttaacgttg aggccttctt cgagcagcag
     2281 gtcgatgcct ctcagcgaag ctttaatgcc ggcagcgtct ccgtcgtaaa gcacggtgat
     2341 gttgtcggta aaacggtgga taagttggat ttgtcctttg gtcagggctg tgcccgacga
     2401 tgctaccacg ttttcgacac cggactggtg caaggaaagc acatcggtat aaccttccac
     2461 caggtaacaa cgatcttgtt tgacgatggc ttgcctggca aaataaatcc cgtacaattc
     2521 ggcacttttg ctgtatatct ccgattctgg agaattgatg tatttagctg ctttggcgtc
     2581 ctttttaaga atacgccctc cgaaggccct tattttgccc gaaatgccgt gtatgggaaa
     2641 aatcaccctg ccccggtaac ggtcgaacaa ctgtccctgt tcgtcggcta cagacaggcc
     2701 ggttttgagc agaaagtctt tttggtagcc ttttgccaag gcattttttg tgaaagcgtc
     2761 gcgttcatcg ggagcatagc ccaactggaa tttttctatg gtatcttcgc gcagacctct
     2821 ttctaaaaaa tatttaagac ctatactcat gccttcgcct gctttcttta aacaggattt
     2881 aaagaaatcc cgggcaaatt cgttgagtac aaacatactt tcccgctcgt ttttgctttg
     2941 cagttcttcg gggctaagtt gtttttctac aacttcgata tggtattttt tagccagcca
     3001 ctttagtgcc tcgtaataac tgagttgttc gtgttccata atgaaatgaa ccacattacc
     3061 tcccttgccg caactaaagc atttgcaaat gcctttggcc ggagaaacac tgaatgaggg
     3121 cgttttgtcg tcgtgaaaag ggcagaggcc taggtagttt actcctcttt tgcgtaaatg
     3181 aacaaattct cccactacct cctctatgcg ggctgcgtct aatattctgt ttatggtgat
     3241 ttgatcaatc atgctcgaag gtgtgccggg gtgctccgat aacttagtta aaaatacgac
     3301 tgttaggttt aacgaaagtc aaattacaca ggtgtttagg gcaataacta aaattatcaa
     3361 aaatatcgct aaatcgaatt aggtgtatta agtatttcat ttatctttgc gggcaaaatt
     3421 aatacataaa tgccaatatt gataaattca gtatggattt ttccccaaga tataccttga
     3481 agtacaccga aagtactaaa tttacgtcct cgataacatc cctttgtata gcactcctta
     3541 tacaataccc ttaattatta cgtcgaaaat taactaaaat aacgcctttt aattcataga
     3601 gtatgtttaa aaacacgcac agagcctggt tgtttctact ttcattgctc ttggtcgttg
     3661 gagcttgttc cgatccttat ccctacgacg acaaagaacc cgactggctc ggttccagca
     3721 tctacgatta tttagtagaa aagggcgaat acacctattt cacaaggatc atagatactt
     3781 gtaattaccg tgaagtattg gccaaaacag gaagcaagac gatgtttgtc tgcaaggatt
     3841 ccgccttcga ggagtatttc agaaagaaca cggacggcat aagtcgtttc gaagatttca
     3901 gtgtagagca actgaatcag ataatggaat acgctatggt aaacgatgcc tccttgatcg
     3961 aaaccttgtc ttatgccgta ggttacgtta aaggccaggt gatgcgccgt gccacttcgc
     4021 tcgacccctt tgatatgctt tcttttgagc agggagatga aattccgaag aacaagtatt
     4081 ttgagcctta tcgtgaaaag ggtttatata ttttgaacga tgacacctcg cccaccttaa
     4141 ttcatttttt tgaggctcag atgaaagaaa agggcattac cgacgaagat tttagcattc
     4201 tcttcaacgg taaaacacgt cagaccaacg atgctcactt attcggtatt aaggtgatcg
     4261 aaagggacat cacttgtaaa aacggttata ttcatgtgct ggaaagccta atgttgccgc
     4321 gcgacaacat ggccggttac atccggaaat ccgacagcct gtctatcttc gcttctctga
     4381 tggatcgttt cagcgcacct tattacactt cttccggtac cgaattatac aaaaccctgc
     4441 atccggaatt cagggattcc atctttgtgc gtaattattt ccaaaaggta agcggtacat
     4501 cgggcgcgta ttccatttta gatcccaacg gcgaattgat tccttccgat gaattgctcc
     4561 gctacgaccc gggctggaat tcttatcaat acacttcatt gtccatggat atgggtgcga
     4621 tgtttgttcc cgacgacgat gcgatgaaat tttatttttc tcccgaaggg aacggctctt
     4681 ttttgcatga acgttataag gtttgggaca gcgttcccaa cgacatagtc gccatgttca
     4741 tcaatgccca catgaagtat tccttcatca gttcggtgcc cagcaagttt cagggtctca
     4801 aggacgaaga cggttttgag attggcatag aaaaagacaa tatccttcgt gccagggttt
     4861 tatgcaacgg agccgtatat gttaccaacg aagtctttcc tccggtagat tatgcttctg
     4921 taatggctcc cgttttggtc aatgagcgga ctcaaatatt taactttgct ctcagaaact
     4981 tggatttcaa tatatacctg agatctctgg aaagctttta cagcttcctg gttcccttcg
     5041 acgaatcctt taccacctat ctctatcctg ccggacaggg acacgaaacc aagaagatga
     5101 tcaaactcaa attcaatact acttccgaaa tggttgatgc tgtaacatac acctacgatg
     5161 atgccacagg tcaactaaaa gattccataa acgtggtatc cagcgcgatt gccctcgact
     5221 gcctgaacga tattatggat tatcatattg tggtgggaga cattgaatcg gggcaggaat
     5281 attatcaaac caaaggaaaa ggagaaatcc gtgttcaggg cagcggtata ggcatgaaag
     5341 tatggggcgg cggcaatatg gagatgaacc ggcgctgggg cagtagctgg gacgacaaca
     5401 aatacacagg tgaagtgacc gatatcctga atttgaagaa cggtaaaacc tactttgtct
     5461 ccaatttatt gcaacagccc atcacttcgg tctataaaac attgagcaac caacccgaat
     5521 tttccaaatt tctgtcggcc atgatgggag ttcccgacga catgaaagag catttgatgc
     5581 tgaccaaaga tcccagaacc ccggggctgg atttcaatat caactttttc tcggcctttc
     5641 actataccct ttatgcgcct aataatgcgg ctatggacag ggcctatgcg gccggtttac
     5701 ccacctggga tcaaattgcc gccgtaccta cagccgacga cgagggcgcc aaaagggctg
     5761 ccatgataca gaaagtggct cgtttcctga agtatcattt ccaggacaac tcggtttata
     5821 ttggtggaga attgctaagt gacgtaaagt acgagacggc gaccaaaaat aatattacca
     5881 ataagt
//
LOCUS       DAOSGL010000112         5834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12103_length_5834_cov_4.598893, whole genome shotgun sequence.
ACCESSION   DAOSGL010000112 DAOSGL010000000
VERSION     DAOSGL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12103_length_5834_cov_4.598893"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKY68_04660"
     CDS             <1..393
                     /locus_tag="PKY68_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOH24362.1"
                     /translation="YTYPYTDKNTFTPEYLNAKRDSVMKINIPGGPEGSYMSTQEIDP
                     PIFRGINVKGKFAAEIRGLWEVKGDMMGGPFVSLTRLDEVNQRVVTVEIFIYAPEEDK
                     RNLLRHNEAALYSLELPGEFELETEEQK"
     gene            403..1494
                     /gene="pdxA"
                     /locus_tag="PKY68_04665"
     CDS             403..1494
                     /gene="pdxA"
                     /locus_tag="PKY68_04665"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOH24363.1"
                     /translation="MEDNKLIIGITQGDINSISYEVILKTLLESKVYEPNAVVIYGSA
                     KVAAYYKKALDINQLSINVINSVQEAEWGAVNLINCTDDDVKVEFGKSTPIAGKASFQ
                     ALEAATVDMKAGLIDVLVTGPINKKNIQSSTFQFPGHTEYLEQVFGNGDKALMLMVSD
                     VLRVAVATGHIALSEVPSALSVPLLVEKIKLLDQSLKKDFGVIKPRIAVLALNPHAGE
                     EGLLGREEIEIIAPAIQKAQDQGCYCFGPIAADGFFGADYFDRYDAILAMYHDQGLIP
                     FKALCRDEGVNFTAGLPIVRTSPAHGTAYALAGNNEASESSFRSALYLACDVYRNRVR
                     YFENNKNPLKKQHIVHSGDLSDLPVQDEE"
     gene            1494..2747
                     /locus_tag="PKY68_04670"
     CDS             1494..2747
                     /locus_tag="PKY68_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOH24364.1"
                     /translation="MAAVDLLKVKQRFGIIGNAAGLNRAIDVAVQVAATDLSVLITGE
                     SGVGKESFPQILHQYSHRKHGPYIAVNCGAIPEGTMDSELFGHEKGSFTGALAERKGY
                     FEVADGGTIFLDEVGDLPIPTQARLLRVLESGEFIRVGSSKTQKTNVRIVAATNIDVP
                     RAVQTGRFREDLYYRLNTVPIKIPPLRERGEDVVRLFRKFAADFSEKYRMPPIQLQED
                     AQQLLQAYRWPGNIRQLKNITEQISIIERQRNISADILEKYLPENSFEKTPAIYRGHQ
                     TSSQAGFFNSEREILYQVLFDMKKDMRDLKVLVHDLMQNAGMEEGLTRSKDLIYPTYI
                     DKTTELISKRDDSSSGKNVRAQDMPQIQDAAEYVEESLSLDELEREAIKKALERNGGR
                     RKSAAADLNISERTLYRKIKEYNLD"
     gene            2750..3250
                     /locus_tag="PKY68_04675"
     CDS             2750..3250
                     /locus_tag="PKY68_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOH24365.1"
                     /translation="MKTRLLLLVLLLLQACTISYKFNAASIDYTTIKSISIKDFPNQA
                     VLVYPPLSQRFSEGLRDIYTQQTRLKVLKQDGDLRVEGEITGYELSSLAAQADGYAAE
                     TKLTVTVNVRYENTKNEKENFEQRFSAFQTFSSTSMLEDVQDALLEEIIKEITESIFN
                     KTVANW"
     gene            3252..4016
                     /locus_tag="PKY68_04680"
     CDS             3252..4016
                     /locus_tag="PKY68_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH24366.1"
                     /translation="MSPETLRNWIETPGLMDRHSQSDLKLLLRNYPCFAGAQVLLLKV
                     MEQEGDLNFSEELFKTSLMVPNRKALYYFMKELPYPPETAGTTGQSDFGLVDQFLEEY
                     TGDGEQLQVEMPDYFQLNTQQSDQAGSKQAPFGKQEDRASSQHTPLGKQEEPTGSRSE
                     KQEEQDLIDKFLSQPAEHIQIDKSYEEQVPETAPVDEYPEASFTETLAKIYLKQGKYE
                     KALEIFNKLLLKYPEKSIYFADQIRFLDKLIHHLKK"
     gene            4022..4345
                     /gene="secG"
                     /locus_tag="PKY68_04685"
     CDS             4022..4345
                     /gene="secG"
                     /locus_tag="PKY68_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOH24367.1"
                     /translation="MYLAITILIFIVAILLILIVLVQNSKGGGLASGFASSNQVMGVR
                     KTTDFLEKATWTLVGIVLFGSILSAAFIDRTSTNERQSALKEQVDQAVEMNPNLTAPS
                     FEDEE"
     gene            4425..5567
                     /locus_tag="PKY68_04690"
     CDS             4425..5567
                     /locus_tag="PKY68_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH24368.1"
                     /translation="MKHLVFCLLAVVMLLSSCGQRRAGQVAFYDENPDSVMTLYYARG
                     FEVSYFSGYTRVQVFNPWADKEVMARYYLYREDNVSLPKDGQAIRIPLNSLAIASCTH
                     VAFLHLLDELELVKGVCNASMVYNPRLKQAIEEGEVADLGDFFQINREKLLHIRPQAL
                     MASGRNQYDEQLGAISRAGVPVIYNNEWMEQNLLGRAEWLRFVACFLDKESLADSLFR
                     QTAANYLRLKELAAQAQSPNPVIMSGDNFRGSWYMPGGRNHQAQLFRDAGASYFFEND
                     STTGSIACSFEQVVRDFNQADIWIGASNARSLSALLALDERYAWFRSVQNKRVYAYQG
                     RLAAGGANDYWESAVAHPDSLLADFIKLFHPELLPGHEWIYINQLQ"
     gene            5564..>5834
                     /locus_tag="PKY68_04695"
     CDS             5564..>5834
                     /locus_tag="PKY68_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOH24369.1"
                     /translation="MKSRTAVFVILSVLLLLLAVADLCWGSIAIPLKDILAAFSKKAS
                     AGVLGEIVLNYRLPKVLTAIVAGSALSVAGLMMQTLFRNPLAGPDV"
BASE COUNT         1602 a         1272 c         1418 g         1542 t
ORIGIN      
        1 tatacctatc cttacaccga caaaaacacc tttacccccg aatacctgaa cgccaaaaga
       61 gattcggtga tgaaaataaa tatacccgga ggtcccgagg gttcctatat gagtactcag
      121 gagatagatc ctccgatatt taggggcata aacgtaaaag gaaaattcgc cgccgaaata
      181 cgcggtttat gggaagtaaa aggcgatatg atgggaggtc cttttgtaag cctgacccgt
      241 cttgacgaag tgaaccaaag agtagtgact gtagagatct tcatatacgc tcccgaagag
      301 gataaacgaa atctcttgcg acacaacgaa gccgccctgt attcattgga actcccggga
      361 gaatttgaat tggaaacaga agaacaaaaa taacagaact aaatggaaga caataaatta
      421 ataataggta ttactcaagg tgacataaac agcatatcct acgaagttat tctcaagacc
      481 ttgttggagt caaaagtgta tgaaccgaat gccgtagtga tttacggctc ggccaaggtg
      541 gctgcttatt ataaaaaagc gctagatatc aatcagttaa gtattaatgt gatcaactca
      601 gtccaggagg ccgagtgggg agccgtcaac ttgataaatt gtacagacga cgatgtgaag
      661 gtggaattcg gtaaatccac ccctatagcc ggcaaggcct cttttcaagc cctggaagcc
      721 gctacggtcg atatgaaggc cggcttgatc gatgtactgg taacgggccc catcaataaa
      781 aagaacattc aatcctctac atttcagttt cccggtcata cggaatacct cgaacaggtg
      841 tttggtaatg gagacaaggc tttgatgctt atggtgagcg atgtcttgcg tgtggcagtg
      901 gctaccggtc atatcgcttt gtccgaagta ccttcggctt tgtcggttcc gctcttggtg
      961 gaaaaaatca agttattgga tcagtcgctc aaaaaagatt ttggggtgat caagccccgc
     1021 attgccgtac ttgccctgaa tccgcatgcc ggtgaagaag gcttgctggg tagggaagag
     1081 attgagatta tcgcccccgc tatacaaaaa gcccaggatc aaggctgcta ttgttttgga
     1141 ccgatagctg ccgacggttt tttcggggct gattattttg accgctacga tgctatcctg
     1201 gccatgtacc acgatcaggg gctcattcca tttaaagcct tatgccggga cgaaggggtt
     1261 aattttaccg ccggtttacc catagtacgt acttcgccgg cacatggcac cgcttacgcg
     1321 ctggcaggca acaatgaggc ttccgaaagt tctttcagat cggctttgta cctggcctgt
     1381 gatgtttaca ggaatcgtgt tcgttatttc gaaaacaaca aaaatccact taaaaaacaa
     1441 catattgtcc attcgggcga tttgtccgat ttacctgttc aagatgaaga ataatggcag
     1501 cagtcgattt attaaaagtt aagcagcgtt tcggtatcat agggaacgcg gccggtttga
     1561 accgggctat agatgtagct gttcaagtgg ctgccaccga tctttccgta ttaattaccg
     1621 gcgagagcgg ggtgggcaaa gagagttttc ctcagatttt gcatcaatac agtcatcgta
     1681 aacacggccc ctacattgcc gtcaactgcg gagccatacc cgaaggcacc atggattcgg
     1741 aattgtttgg ccatgaaaaa ggctctttta ccggggcgct ggccgaacgc aaaggttatt
     1801 tcgaagtggc ggacggaggc accatttttt tagatgaagt gggagattta cctattccca
     1861 cccaggcccg tctgctcaga gtattggaat cgggtgagtt catacgtgtg ggttcttcta
     1921 aaactcaaaa aactaatgta agaattgtgg ctgccaccaa cattgatgtt ccccgggcag
     1981 tgcaaaccgg tcgcttcaga gaagatttat attaccgttt aaatactgta cctatcaaga
     2041 ttccgccctt gcgtgaacgg ggcgaagatg tcgttcgact atttagaaaa tttgcagccg
     2101 atttttcaga aaaataccgt atgcctccca tacaactcca ggaagatgca caacaacttt
     2161 tacaggccta tcggtggccg ggaaatattc gtcagctgaa aaacataacc gagcaaattt
     2221 caattattga acggcaacgc aatatttcgg ccgatatttt agagaaatac ctgcccgaga
     2281 attctttcga gaaaaccccg gctatttaca gaggccatca aacctcttct caagccggct
     2341 ttttcaacag cgaaagagaa attttgtacc aggtcttatt cgatatgaaa aaggacatga
     2401 gagatttgaa agtgctggtt cacgatttga tgcaaaatgc cggcatggaa gaaggattga
     2461 cacgcagcaa agacctgatt tatcccacct atattgataa aacaactgaa ctgatcagca
     2521 aacgggacga tagctcaagc ggtaaaaacg tgcgtgccca agacatgcct cagattcagg
     2581 atgcggccga gtatgtggag gaatctcttt cgctggatga acttgagcgc gaagctatta
     2641 aaaaggctct ggagcgcaac ggaggaaggc ggaaaagcgc tgctgcagat ttaaatattt
     2701 ccgaaaggac cctatatcgc aaaatcaagg aatacaacct ggactgatta tgaaaacgcg
     2761 tcttttatta cttgttctgc tcttgcttca ggcctgtact atttcatata agttcaatgc
     2821 tgcttccatt gattatacta ccataaagtc gatttccatc aaagacttcc ccaatcaggc
     2881 cgtgttggtg tatcctcctc tttctcagag gttttccgag gggttaaggg atatttatac
     2941 gcaacaaacc aggcttaagg tgttgaagca agacggagat cttcgtgttg aaggcgaaat
     3001 cacaggctat gaactaagct cgctggcagc acaggccgac ggctatgcgg ccgaaactaa
     3061 attgaccgta acggtcaatg tgagatacga aaacactaaa aacgaaaagg aaaattttga
     3121 acagcgtttc agcgcttttc aaacctttag cagcacttcc atgctcgaag acgtccagga
     3181 cgctctttta gaggaaatca tcaaagaaat taccgaaagc atattcaaca aaacagtagc
     3241 caactggtag catgagcccc gaaacactta ggaactggat agaaactccc ggattgatgg
     3301 atcgtcacag ccagagtgac cttaaattgc ttttgcggaa ctacccttgt tttgccgggg
     3361 cccaggtttt gttactgaaa gttatggagc aggagggcga tttgaatttt tcggaagaat
     3421 tgttcaaaac ctcattgatg gtgcccaaca gaaaggcttt gtattatttc atgaaagaat
     3481 tgccttatcc gccggaaact gccggcacaa ccgggcagtc ggattttggt ttggttgatc
     3541 agtttctgga agaatacact ggtgacgggg agcaacttca ggtcgaaatg cccgattatt
     3601 ttcaactgaa cactcaacaa tccgatcaag ccggcagcaa gcaggctccg ttcggcaaac
     3661 aggaggaccg ggcttccagc cagcacactc ccttgggcaa gcaggaggag ccaaccggca
     3721 gccggtccga aaagcaggag gaacaggatc tgatcgacaa attcctcagt cagcccgcag
     3781 aacatataca aatagacaaa tcatacgaag aacaagtgcc ggaaaccgct ccggttgacg
     3841 aatatccgga ggcctctttt actgagaccc tggccaaaat ctacctgaaa cagggcaaat
     3901 acgaaaaagc tcttgaaata ttcaacaaat tgttattgaa atatccggaa aaaagtattt
     3961 actttgcaga ccaaatccga tttttagata aactgattca tcatttaaaa aaatagataa
     4021 tatgtacctg gccatcacca ttttaatctt tatcgtagcc atacttctca ttcttatcgt
     4081 tttggttcaa aactccaagg gtggcggatt ggcctcgggt tttgcttctt ccaatcaggt
     4141 aatgggtgtg cgcaaaacca ccgatttttt ggaaaaagcc acctggactt tggttggtat
     4201 tgtgcttttc ggcagtattc tttctgcagc atttattgat cgcaccagca ctaacgaacg
     4261 tcagtctgct ctaaaagaac aagtggacca agccgtggaa atgaatccca atctgacggc
     4321 tcccagtttt gaggatgaag aataaatgct tactataaaa tgctataaga gggcgtcatc
     4381 cgcaggttga ctcccttttt tatttctttt tgcggcctgt ttttatgaaa caccttgttt
     4441 tttgcctttt agcggtcgtt atgctgttga gttcctgtgg tcaacggagg gcaggacaag
     4501 ttgcgttcta cgatgaaaat cccgattccg taatgacact gtattacgct cgtggttttg
     4561 aggtcagtta ctttagcggc tataccagag ttcaagtgtt taatccctgg gcagacaagg
     4621 aggtcatggc ccgttactat ctttacaggg aagacaacgt atccttgccc aaagacggac
     4681 aggctatccg tatcccccta aattcactgg ctatagcttc ttgcacccac gtggcttttt
     4741 tgcatttgct cgatgaactt gagctggtca agggcgtgtg caatgcttct atggtttata
     4801 atcctcgttt gaagcaggcc attgaagagg gagaggtggc cgatttgggc gatttctttc
     4861 agatcaaccg tgaaaagctg cttcatatac gtcctcaggc tcttatggcc agcggtcgca
     4921 atcaatacga cgagcaatta ggggccattt cccgggctgg tgtgcctgtt atctacaaca
     4981 atgaatggat ggagcaaaat ttgctgggca gggcagaatg gctgcgtttt gtagcctgct
     5041 ttctggataa agagagcctg gccgattcac ttttcagaca gaccgcagcc aattatttgc
     5101 gtttaaagga actggcagca caggctcaaa gccccaatcc cgttattatg tcgggtgaca
     5161 atttcagggg cagttggtat atgcccgggg ggcggaatca tcaggcccaa ctgttcagag
     5221 acgccggagc ctcttacttt tttgaaaacg atagcacaac cggcagtatt gcctgtagtt
     5281 ttgaacaagt tgtcagagat tttaatcagg cagatatttg gataggggct agtaatgccc
     5341 gtagcctgag tgctctgttg gctctggatg agcgttatgc ctggttcagg tccgtacaga
     5401 ataagcgggt gtatgcatac caggggcgtc ttgctgcggg gggtgctaat gattattggg
     5461 aaagtgcggt ggctcatccg gattctttat tggccgactt tattaagttg tttcatcccg
     5521 aacttttgcc cgggcatgag tggatctata taaatcagtt gcaatgaagt cccggactgc
     5581 tgtattcgtg attttgtccg tattgctgct gttgctggca gtggccgatt tgtgttgggg
     5641 aagtattgcc ataccactca aggatatttt ggcggctttt agtaagaaag catccgccgg
     5701 agttcttgga gaaattgtcc tgaattatcg tttacctaaa gtgctgaccg ctattgtggc
     5761 cggttcagct ttgtcggtgg cgggtttgat gatgcagact ttgtttcgta atcctttagc
     5821 cggtcctgat gtgc
//
LOCUS       DAOSGL010000113         5832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12107_length_5832_cov_3.361433, whole genome shotgun sequence.
ACCESSION   DAOSGL010000113 DAOSGL010000000
VERSION     DAOSGL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12107_length_5832_cov_3.361433"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1708
                     /locus_tag="PKY68_04700"
     CDS             212..1708
                     /locus_tag="PKY68_04700"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOH24370.1"
                     /translation="MSDKVFIFDTTLRDGEQVPGCQLNTVEKIQVAKALESLGVDVIE
                     AGFPVSSPGDFNSVVEISKAVTWPTICALTRSVEKDIDVAAEALKYAKRSRIHTGIGT
                     SDYHIKYKFNSNRDEILQRAIACVKYAKKYVEDVEFYAEDAGRTDNEYLARVVEAVIK
                     AGATVVNIPDTTGYCLPDVYGAKIRYLMENVPNIHKAVISTHCHNDLGMATANTMSGI
                     LNGARQVEVAVNGVGERAGNTSLEEIAMILRCHKNLGVHTDINTQHIMHSSRLVSSLM
                     NMPVQPNKAVVGRNAFAHSSGIHQDGVLKNRENYEIMDPKDVGIDENSIVLTARSGRA
                     ALKHRLQLLGVEIGDERLQEVYQEFLKLADRKKVINDEDILLLTGEERSEKKRIKLDH
                     LQVVSGKGVRAVASITLDIGGEKFEATASGNGPVDAGIKALKRIINREMTLQEFLIQA
                     IAKGSDDTGKVHMQVEHQGSVYYGFSGNTDIITASIEAYIDAINKFVD"
     gene            1722..3116
                     /gene="leuC"
                     /locus_tag="PKY68_04705"
     CDS             1722..3116
                     /gene="leuC"
                     /locus_tag="PKY68_04705"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOH24371.1"
                     /translation="MGKTLFDKIWDRHVITTVPDGPTQLYIDRHYCHEVTSPQAFQGL
                     RDRKLRFLRPEKTYAIPDHNIPTLNQDQPIQDPVSRKQVETLQENAREFGIKYFGLGH
                     PKNGVIHVVGPETGLTLPGMTIVCGDSHTSTHGALGAVAFGIGTSEVEMVMASQSILQ
                     TKPKKMRIRVEGKLRPGVSAKDMALYLIAKMTTGGATGYFVEYAGEAVRALSMEGRLT
                     LCNLSIEMGARGGMIAPDQTTFDYIKGREFAPKGAAWQQAVEKWKTLYSDEDAVFDKE
                     VQFKAEDIEPMITYGTNPGMGMAITGRIPLLESIDPSGRASFVKAMDYMGFKPGETLL
                     GKKIDYVFLGSCTNGRIEDFRTFTQFVRGRQKAPGVVAWLVPGSKAVEQQCREEGLTE
                     ILEQAGFVLRQPGCSACLAMNDDKIPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAA
                     AAAVTGLITDPRTY"
     gene            3117..3707
                     /gene="leuD"
                     /locus_tag="PKY68_04710"
     CDS             3117..3707
                     /gene="leuD"
                     /locus_tag="PKY68_04710"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOH24372.1"
                     /translation="MMKKFTTLTSTVVPLPMENVDTDQIIPARFLKATSKEGFGDNLF
                     RDWRYDGNNRPNPDFVLNNPLYGGSILVAGKNFGSGSSREHAAWALAGYGFRVVVSSF
                     FADIFKNNALNSGVLPVVVSEAFLQDIFDTVYADAKSTLTVDLEKQVIRNNVKGREES
                     FSINAYKKNCLLQGYDDIDFLLSNKDKIQAWENEND"
     gene            3694..5217
                     /locus_tag="PKY68_04715"
     CDS             3694..5217
                     /locus_tag="PKY68_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOH24373.1"
                     /translation="MKTIEILDTTLRDGEQTSGVSFGVQEKLSIARLLLEELKVDRIE
                     VASARVSEGEFKAVRRISEWAGERGLLDRIEILGFIDKGLSLQWASDAGCRVINLLSK
                     GSYRHVTEQLKKTPEQHLKDIKDEVALCRAKGVQVNLYLEDWSNGMMHSPDYVFYLMD
                     SLRDETIGHFMLPDTLGILTPEQTSLYCRQMVERYPGLKFDFHAHNDYDLAVSNVFQA
                     VRAGVTTVHTTVNGLGERAGNAPITSVIAVLHDLLKVQTGIREDRVNRVSRIVESYSG
                     VRIPANKPVIGDNVFTQTAGIHADGDVKSNLYFNDLLPERFGRVRQYALGKTSGKASI
                     KKNLEALGIELDDDAMRLVTERIIQLGDKKELVTQEDLPYIISDVLKQNHQEEPVKVL
                     NYALSLAHGLRPVATVMLDICGEVFQETASGDGQYDAFMKALHKIYSSQKRNLPQLVN
                     YVVTIPPGGRTDAFVQTVIFWSYNNKSFRTSGLDADQTEAAIKATMKMLNIIEEMNI"
     gene            5324..>5832
                     /locus_tag="PKY68_04720"
     CDS             5324..>5832
                     /locus_tag="PKY68_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOH24374.1"
                     /translation="MKLNIAVLPGDGIGPEIVEQALNVTKAVCEKFGHELNYKTGLVG
                     AAAIDACGDPYPEETHKLCMDSDAVLFGAVGSLKYDNDPKAKIRPEQGLLSMRKKLGL
                     YANIRPVSVFPSLISRSPLKNRLVRNVDFLCIRELTGGIYFGRPQGRSEDGNSAVDTC
                     TYSREEIER"
BASE COUNT         1606 a         1377 c         1470 g         1379 t
ORIGIN      
        1 ataaaaaata gctatatttg cggccgaaag taaacaaaac tatgatccat acggctctga
       61 tttccctgat tctaattgct atactactgt tcggcaggta ggtagtgaga caggtgtata
      121 tatcggagaa cgacgatcga aaaatataga gctcttcttg ctaccaggca ggaggagctt
      181 tttttatgaa ctaattttaa tgctaatgga tatgtcagac aaagttttta ttttcgatac
      241 caccctgcgc gatggcgagc aggtgccggg ttgtcagttg aatacggtag aaaagattca
      301 ggtggccaaa gccctcgagt ctttgggtgt ggacgttatt gaggcgggtt ttccggtatc
      361 gagtccgggc gattttaatt cggtggtgga aatttccaag gctgttacct ggccgaccat
      421 ttgtgctctg acccgttcgg tcgaaaaaga catcgacgtg gctgccgagg ctcttaaata
      481 cgccaagaga agccgcattc acaccggtat cggcacgtcg gattaccaca tcaaatacaa
      541 attcaactcc aaccgcgacg agattttaca aagggctatc gcttgcgtta aatacgccaa
      601 gaagtacgtc gaagacgtgg agttttatgc cgaagatgcc ggccgtaccg ataacgaata
      661 cctggcccgt gtggtagaag ctgttattaa agccggagcc acggttgtaa atattcccga
      721 caccaccggt tattgtttgc ccgatgtgta cggagccaaa atccgttatt tgatggaaaa
      781 tgtgcccaat atccacaagg ctgttatttc aactcattgt cacaacgacc tgggcatggc
      841 aacagccaac accatgtcgg gcatactaaa tggcgcccgt caggtggaag tcgccgtcaa
      901 cggcgttggc gagagggccg gcaatacttc tctcgaagaa atagccatga tacttcgctg
      961 tcacaaaaac ctgggcgtgc ataccgacat caacacccag catattatgc acagcagccg
     1021 cctggtgtcg agcttgatga atatgccggt gcagcccaac aaagctgtag tgggccgtaa
     1081 tgcttttgct cattcctcgg gtattcatca ggacggggtg ttgaaaaacc gcgaaaatta
     1141 cgaaatcatg gaccccaaag acgtgggtat agacgaaaat tccattgtgt tgactgcccg
     1201 tagcggacgt gcggcgctga aacaccgctt acagcttttg ggagtggaga ttggggacga
     1261 acgtttgcaa gaagtttatc aggaatttct taaattagcc gatcgaaaaa aagtcatcaa
     1321 cgacgaggat attttactgc tgacaggcga ggagagaagc gagaaaaaac gcatcaaact
     1381 cgatcatctc caggtggttt cgggcaaggg tgtaagagcg gtagccagta taaccctgga
     1441 tattggcggt gaaaaatttg aagccactgc ttcgggcaac ggaccggtgg acgccggaat
     1501 caaggcactg aaacgcataa taaaccggga aatgaccctg caggaattcc tgattcaggc
     1561 catagccaag ggcagcgacg acaccggtaa ggtacacatg caggtagaac accagggctc
     1621 tgtctattac ggattttctg gtaataccga cataattacc gcctctatcg aagcttatat
     1681 cgacgccatc aataaatttg tagactgaaa ataatacgag catgggaaaa accttgttcg
     1741 ataaaatatg ggacaggcac gtaatcacta ctgtgcccga cgggcctacc caattgtaca
     1801 tcgaccggca ttattgccac gaagttacca gtccccaggc ctttcagggt ttgagagaca
     1861 gaaagctgcg ctttttgcgt cccgaaaaaa cctacgccat ccctgatcac aatattccta
     1921 ccctgaatca ggaccagcct attcaggatc ctgtttcgcg caagcaggta gagaccttgc
     1981 aggaaaacgc ccgggaattc ggtattaagt attttgggct gggacatccc aaaaacggtg
     2041 tgattcatgt ggtagggcct gaaaccggtc ttactttgcc aggcatgacc atagtctgcg
     2101 gagattcgca tacttccact cacggcgctt tgggagcagt agcctttggc ataggaactt
     2161 ccgaagtaga gatggtgatg gcgtctcaaa gcatcttgca gaccaagcca aagaaaatgc
     2221 gcatcagggt agagggtaaa ctccgtccgg gcgtaagcgc caaagatatg gccttgtatc
     2281 tcattgcaaa aatgaccacc ggaggtgcca ccggttattt tgtggaatac gccggagagg
     2341 ccgtgcgggc tttgagcatg gagggtcgcc ttacgctttg caacctgtcc atcgaaatgg
     2401 gagccagggg cggtatgatt gctcccgatc aaaccacttt cgattacatc aaaggccggg
     2461 aatttgctcc caagggagcg gcctggcagc aagcagtaga gaaatggaaa acgctctaca
     2521 gcgacgagga cgccgttttc gataaagagg tacaattcaa ggccgaagat atagagccca
     2581 tgattaccta tggtaccaac ccgggtatgg ggatggctat tactgggcgt attcctttgt
     2641 tggaaagcat cgatccttcg ggcagggcct cctttgtcaa agctatggac tacatgggct
     2701 ttaaaccggg agaaactttg ttggggaaaa agatcgatta cgtctttctg ggcagttgta
     2761 ccaacggcag aatagaagat ttcagaactt ttacccaatt cgtccgcgga cgtcaaaaag
     2821 ctcccggtgt tgtggcctgg ctggtacccg gctccaaagc ggtagagcaa caatgccgcg
     2881 aagagggttt gacggaaatt ctggagcaag ccggttttgt ccttaggcaa cccggttgtt
     2941 cggcttgtct ggctatgaac gacgataaga ttcccgccgg caaatacgct gtttccacat
     3001 ccaaccgcaa tttcgaaggc cgtcagggac ccggagccag aaccatactg gcaggtcccc
     3061 tggttgcggc ggcggcagcc gtcaccggcc ttattaccga cccaagaact tattgaatca
     3121 tgaaaaaatt tactacactt acttccaccg tcgttccact gcccatggaa aatgtggata
     3181 ccgatcaaat catacctgcc cgttttctga aagctacttc caaagaaggc tttggcgata
     3241 atcttttcag agattggcgt tacgacggca acaatcgacc caatccggat tttgtgttga
     3301 acaatccgct ttacggaggt tcgatactgg tggccggcaa aaacttcggc agcggttcca
     3361 gtcgcgaaca cgcagcctgg gcattggccg gttacggctt tcgtgtggtg gtatcgagct
     3421 tctttgccga tattttcaaa aacaatgccc tcaatagcgg agttttaccc gtggtggtga
     3481 gcgaagcttt tcttcaggat attttcgata ccgtctatgc cgatgcgaaa agcaccctta
     3541 cggtggatct ggaaaagcaa gtcattcgca acaacgttaa gggccgcgaa gaatcattca
     3601 gtatcaacgc ttacaaaaag aattgtctgc tgcaaggcta cgacgacatc gactttttgc
     3661 tgagtaacaa agacaagatt caagcctggg aaaatgaaaa cgattgaaat tttagatact
     3721 acgttgagag acggcgaaca aacttcgggt gtaagttttg gagtgcagga aaaattaagc
     3781 attgccaggc tgttgctcga agagctcaaa gttgatcgta tagaagtggc ttctgcgagg
     3841 gtctcagagg gtgaattcaa agctgtgcgg cgtatctcgg aatgggccgg tgagcgtggt
     3901 ttgctcgatc gtattgaaat tctgggcttt atagataaag gcttgtcgtt gcaatgggcc
     3961 tcggatgcag gttgcagggt gatcaacttg ctttccaaag gttcataccg gcatgtcact
     4021 gagcaattga aaaaaactcc cgaacaacac ttaaaggata tcaaagacga agtagccctg
     4081 tgcagagcca agggggtgca agtgaacctc tatctcgaag actggtccaa cggcatgatg
     4141 cattcgcccg actatgtctt ttacttgatg gacagcttgc gcgatgaaac cattgggcat
     4201 tttatgttgc ccgataccct gggtatcctc actcctgaac aaacatccct ttactgccgg
     4261 caaatggtgg aacgttaccc cggattgaaa tttgatttcc atgcccacaa cgattacgat
     4321 ttggccgttt ccaatgtttt tcaggctgtc agggcaggag taactaccgt gcacactacg
     4381 gtaaacggtt tgggcgaaag agctggtaat gctcccatta ccagtgttat tgccgtgttg
     4441 cacgatttgc tgaaagttca aaccggcatc agggaggata gggtcaacag agtaagccgc
     4501 attgtggaat cttattccgg cgtgcgcatt cctgccaaca agccggtaat cggcgacaat
     4561 gtttttaccc agacagcggg cattcacgcc gatggagacg tcaaaagcaa cctttatttc
     4621 aacgatctgc tgcccgaacg ttttggccgt gtacgccagt atgccttggg taaaacctcc
     4681 ggcaaagcga gcatcaaaaa aaacctggaa gctctgggta tagagctcga cgacgacgct
     4741 atgcgtttgg ttaccgaacg tatcattcaa ctgggcgaca aaaaagagct tgttacccaa
     4801 gaagatcttc cctacatcat ctcagacgtg ctgaagcaaa atcaccagga agagcctgtc
     4861 aaagtgctta attacgctct ttcgctggcg catggcttgc gtccggtagc caccgttatg
     4921 ttggatattt gcggtgaggt ttttcaggaa acagccagcg gcgacggaca atacgatgcc
     4981 tttatgaaag ccctgcacaa gatctacagc agccaaaaac gcaatttgcc ccagttggta
     5041 aattacgttg taaccatacc gcccggcgga cgcaccgatg cctttgtaca aaccgttatc
     5101 ttctggtcgt acaataacaa gagcttcaga accagcgggc tggacgcaga ccaaaccgaa
     5161 gccgctatca aagcgaccat gaaaatgctc aatattattg aggaaatgaa tatataaacc
     5221 cattcaaagt tttggaccgt tttttttatt aaatcctttc cttattaaag aacaggaaca
     5281 accaaaaaac taaaaaataa taaaacaata aaacaataag aacatgaaac tgaacattgc
     5341 agtattaccc ggtgacggga taggccccga aattgtggaa caggccttga acgtcaccaa
     5401 agccgtttgc gaaaaattcg gccatgaatt gaattataaa acaggtctgg tgggcgctgc
     5461 cgccatagat gcttgtggtg atccttatcc cgaagaaacc cataaactct gcatggactc
     5521 cgatgccgtg ctgtttggcg ccgtgggttc actcaaatac gacaacgacc ccaaagccaa
     5581 aattcgcccc gaacagggct tgctgtcaat gcggaaaaaa ctgggactct atgccaacat
     5641 tcgtccggtt tcggtttttc ccagtttgat cagccgttcg cccctcaaaa acagattggt
     5701 tcgtaatgtc gattttctat gcatccgcga actcaccggc ggcatttatt tcggacgtcc
     5761 acaagggcgc agcgaagacg gcaacagcgc cgtagatacc tgtacctaca gccgcgaaga
     5821 aatagaacgc at
//
LOCUS       DAOSGL010000114         5825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12121_length_5825_cov_4.637955, whole genome shotgun sequence.
ACCESSION   DAOSGL010000114 DAOSGL010000000
VERSION     DAOSGL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12121_length_5825_cov_4.637955"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PKY68_04725"
     CDS             <1..455
                     /locus_tag="PKY68_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOH24375.1"
                     /translation="TRLPPPVDSDVEQKVEERNTLVVLVSQENKIMCQKKEIELWELK
                     DIAKEFIENPYNDIHLPKKVPVTINFFGQVEVTKPHVISLQNDRSTKYSKYIEVQNEL
                     IAAYNELRDELSLRTFGKPFAELDEERQKAVAEYYPQKISEAEPKEYK"
     gene            461..943
                     /locus_tag="PKY68_04730"
     CDS             461..943
                     /locus_tag="PKY68_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOH24376.1"
                     /translation="MAKFKKSSKGGSQEINASSMSDVIFMLLFFFMTVTTMKEVEYKV
                     QFQLPEATELTKLEKKSLVTYIYIGPPTISNRARMGSESRIQLNDKYAEVSDIQDYIA
                     QEKGNMREEDQPYMTVSLKIDKDTNMGIVNDVKQALRKAYALKISYAATGKEGKQIEH
                     "
     gene            complement(1012..1506)
                     /locus_tag="PKY68_04735"
     CDS             complement(1012..1506)
                     /locus_tag="PKY68_04735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH24377.1"
                     /translation="MFKLRKADAQDAPLINRLAKEVFPLTYKEILSPEQNEFMFEWMY
                     AVDNIHRQMHEGHCYFLLEDEGQACGYMSIQQEEELFHLHKIYVLPVCQKTGAGRFLF
                     EQALAYIKSIHPSPCRLELRVNRKNPAVDFYKKMGMRVLRSDDFDIGRGYFMNDYIMG
                     IDLC"
     gene            complement(1521..2276)
                     /gene="tpiA"
                     /locus_tag="PKY68_04740"
     CDS             complement(1521..2276)
                     /gene="tpiA"
                     /locus_tag="PKY68_04740"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOH24378.1"
                     /translation="MRKKIVAGNWKMNKNLQEGIQLAVALNEVLSKESNSCRVIIGTP
                     FIHLAPVSERIDKKKLGLAAQNCADHVSGAYTGEVSAEMVKSTGAEYVILGHSERRTY
                     YGETLEMLKTKVRLALDQQLQPIFCIGEVLSEREAGKQFEVVRSQIEASLFDLSEADF
                     SKIILAYEPVWAIGTGKTATAAQAQEMHAFIRGLIADKYGKKAADDCSILYGGSCKAS
                     NAAELFANPDVDGGLIGGASLTVEDFMGIVRAR"
     gene            complement(2304..3593)
                     /locus_tag="PKY68_04745"
     CDS             complement(2304..3593)
                     /locus_tag="PKY68_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOH24379.1"
                     /translation="MKKGHKILALVSRLLLGITFVFSGFVKAVDPLGSTYKIQDYLQA
                     FGLGFFDQYALFFSFVLFCLEFVLGMALLLGAAQRFFAWLALVFMSLMTLLTLVIALG
                     NPVSDCGCFGDALQLSNWQTFGKNIFLLLLSLILISFKEPLYKVFGKKTQKWACLVCL
                     LMVLLVAIHSLRHLPMLDFRPYKTGNSIPELMQIPPGAPQDSVVMEYIYEKDGVYQSF
                     GLENYPAGDTTWHFVDRKERIVRKGYEPPIHDFVLLHPVYGDITEAVLSDSSYTFLWI
                     SYKLEKANRSAIQDFLQIQRYAADRGYSFYGLTASGQEIIDEWTYEYDIPFDFCTVDE
                     ITLKTMIRSNPGLMLISNGQILHKWAWRDLSALTMDLDAPLEETPWSKAASNKGLVVA
                     SQFFALVVIALCFLYLFRLASLLWEYKKKKKQIHQNK"
     gene            complement(3597..4139)
                     /locus_tag="PKY68_04750"
     CDS             complement(3597..4139)
                     /locus_tag="PKY68_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOH24380.1"
                     /translation="MSKTNEQFDAVTAKCRRIFSKKLHDYGAAWRILRIPSVTDQLFI
                     KANRIRNIEIKGESKIQDNIADEFMGIINYAIVGLIQLQQGFSEKVDITPEEALALYD
                     EKMEMARQLMLNKNFDYEEAWRGMRISSYTDLILMKINRVKQIEDLGGQTIASEGIDS
                     NYLDMLNYAAFGLIRLGYDK"
     gene            complement(4235..5419)
                     /locus_tag="PKY68_04755"
     CDS             complement(4235..5419)
                     /locus_tag="PKY68_04755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH24381.1"
                     /translation="MKLSVIIVNYNVRYFLEQCLHSLYRAIKNTETEVFVVDNCSTDD
                     SLEMLRQSYPEIKLIANSENLGFAKANNLAIEASESDYVLLLNPDTLVQEEAIRNMLS
                     FMDAHPEAGALGVKMIDGRGRFLAESKRGLPTPAVSFYKISGLSALFPRSRRFGRYHL
                     GWLDKDKIHSVEILAGACMLVRREAIIKAGLLDENFFMYGEDIDWSYRILKAGYRNYY
                     FPPGRILHYKGESTKKGSLNYVYVFYNAMAIFAKKHFLGKNFFYALLINLAIWLSGAF
                     SFFSGLLRKCLYIIKKIAFSTQQASVLVWGGTSEFERVRDLYSTVSTGSLNFIQVTNE
                     DQIEEALKDKDIRGLVFCTAELEYSLVFDCMEKYAGRGLVFKMAPPIGQVIIGSHTVL
                     KP"
     gene            complement(5427..>5825)
                     /locus_tag="PKY68_04760"
     CDS             complement(5427..>5825)
                     /locus_tag="PKY68_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOH24382.1"
                     /translation="ERFGLSQLHQLRGRVGRGADQSYCVLLTSYKLSDEGRKRIEIMV
                     SSNDGFEIAEADLKMRGPGDLEGLQQSGVPFDLKIADLGKDAQILEYARQIAGECLEK
                     DPDLSLPENALMAEELKKRYGKKINWGAIS"
BASE COUNT         1731 a         1356 c         1203 g         1535 t
ORIGIN      
        1 cgacgcgtct tccaccgcca gtggacagcg acgttgagca aaaggtagaa gaacgcaata
       61 ctttggtggt tttggtaagt caggaaaaca agataatgtg ccagaaaaaa gaaattgaac
      121 tatgggagct caaggatatt gcaaaggagt ttatcgaaaa tccctacaac gatattcatc
      181 ttcccaaaaa agttcccgta acaattaatt tttttggcca ggtagaagtg accaaaccac
      241 atgtcatttc cctgcaaaac gaccgtagca ccaaatattc caaatacatt gaagtgcaaa
      301 acgagttaat tgcagcctat aacgaattga gagacgagct ttcgctcaga acttttggga
      361 aaccttttgc agaattggac gaggaacgcc aaaaagcagt tgctgaatat tatcctcaaa
      421 agatttccga agcagaacct aaagaatata aataacgatc atggcaaagt ttaagaaatc
      481 tagcaaaggt ggttcccagg agattaatgc ttcttcgatg tcggacgtta tctttatgtt
      541 acttttcttc tttatgacag taacaacgat gaaggaagtg gaatataaag ttcaatttca
      601 acttcctgag gctaccgaac tcacaaaact ggaaaagaaa tccctggtaa cctatattta
      661 catcggccca cctacgataa gtaaccgtgc ccgtatgggt tcggaatctc gtattcagct
      721 taacgacaag tacgccgaag tttcagatat ccaggattat attgcccagg aaaaaggcaa
      781 tatgagagaa gaagaccaac cctatatgac cgtgtccctt aaaattgaca aggataccaa
      841 tatgggtata gtaaacgatg tcaaacaggc cctaagaaag gcctacgctc ttaaaatcag
      901 ttatgctgcc accggtaagg agggaaagca aatcgaacat taaaataggg agagtaatcg
      961 aactgaaaaa aggctgaaca gattgttgtt cagccttttt tatatgggta attagcacaa
     1021 atctatgccc atgatgtaat cgttcataaa ataaccccgg ccaatatcaa aatcgtcgga
     1081 acgtaacacc ctcatgccca tctttttata gaaatccacg gccggatttt tgcggttgac
     1141 acgcaattcc aaacgacaag gagaggggtg tatgcttttt atgtatgcca aggcttgctc
     1201 aaataaaaag cgtccggctc cggtcttttg gcatacaggc agtacatata tcttatgaag
     1261 gtgaaacagc tcttcttctt gttgaatgga catatacccg caagcctggc cctcgtcttc
     1321 cagaagaaag taacaatggc cctcgtgcat ctgtcgatga atattgtcca ccgcgtacat
     1381 ccattcaaac ataaactcgt tctgctccgg cgagagaatt tccttgtacg tgaggggaaa
     1441 aacttctttt gccagacggt taatcaacgg agcatcctga gcatctgcct tccggagctt
     1501 aaacatcttc gattgttgtt ttagcgggca cgaacaatcc ccataaaatc ttccacggtc
     1561 agcgaagcgc ctcctatcag acctccgtca acgtcggggt tggcaaacaa ttcagcggca
     1621 ttggaggcct tgcaactacc accgtacaaa atggagcagt cgtcggctgc cttcttgccg
     1681 tatttgtcgg caatgagacc gcgaataaag gcatgcattt cctgggcttg tgcagcggtg
     1741 gctgttttgc cggttccgat ggcccaaaca ggctcgtaag ccagaattat tttggagaaa
     1801 tcagcctcgg acaaatcgaa caaagaagct tcaatctgtg aacgaaccac ttcaaattgc
     1861 ttgccggctt cacgctcact caacacctct ccgatgcaga aaatgggctg cagttgctga
     1921 tctagggcaa gacgaacttt agttttgagc atttccagag tttcaccgta ataagttcta
     1981 cgttcggaat gacccaaaat gacatattct gctccggtag atttgaccat ctcggccgag
     2041 acttcacctg tataggctcc actaacatga tctgcgcaat tctgcgcggc aaggcccagt
     2101 tttttcttgt ctattcgttc acttacgggg gccagatgta taaatggtgt gcctataatc
     2161 accctgcagg aattactttc tttggataat acttcgttca gggcaactgc cagttgaata
     2221 ccttcttgca ggttcttgtt cattttccag tttcctgcga caatcttctt tctcatacgt
     2281 tgggtttaaa attgataata taactacttg ttttgatgta tttgcttttt cttctttttg
     2341 tattcccaca acagggatgc cagcctgaac aggtataaaa aacacaaggc gatgactacc
     2401 agggcaaaga attgagatgc taccaccagg cctttgttgg aggcggcttt agaccaaggc
     2461 gtctcctcga gaggagcatc aagatccatg gtgagcgctg acaaatctct ccaggcccat
     2521 ttgtgcagta tctgtccgtt gctgatcagc ataagacccg gatttgaacg aatcatggtt
     2581 ttaagcgtaa tctcatccac tgtgcaaaaa tcgaagggga tatcgtactc gtaggtccat
     2641 tcgtcaataa tttcctgccc ggaagctgtc aaaccgtaaa aggaataacc cctgtctgcc
     2701 gcataacgct gaatttgcag gaaatcctgg atggccgagc ggtttgcctt ttccaattta
     2761 taggaaatcc ataaaaaagt gtaggaacta tcgcttagta ccgcctccgt aatatcaccg
     2821 taaaccggat gaagtaatac aaaatcgtga atgggaggct catagccttt gcgaacgatt
     2881 ctctctttgc gatctacaaa atgccaggtg gtatctccgg ccggataatt ctccaaaccg
     2941 aaagattgat aaaccccgtc tttttcgtaa atgtattcca tcaccaccga atcttgagga
     3001 gctcccgggg gaatctgcat caactcagga atggaattac ctgttttata gggacgaaaa
     3061 tccaacatgg gcaaatgacg caaagaatgg atagcaacca gaagcaccat cagcagacaa
     3121 accaaacagg cccatttttg ggtctttttt ccaaacactt tatatagggg ctctttgaag
     3181 ctgattaaaa taagggacaa cagaagaagg aaaatatttt ttccaaaggt ttgccagttg
     3241 ctcagctgta aggcatctcc aaaacaaccg caatcgctca ccggatttcc caatgcgatc
     3301 accaaggtga gcaaggtcat cagcgacata aacaccaggg ccaaccaggc aaaaaatctt
     3361 tgtgctgccc ccaacaacaa agccataccc agcacaaatt caaggcaaaa aagcacaaag
     3421 gaaaaaaaca gggcatattg atcaaagaaa cccaaaccaa aagcctgcag ataatcctga
     3481 attttatagg tgctacccaa aggatccact gctttgacaa agccggaaaa cacaaaagtt
     3541 attcctaaaa gcaaccttga gactaatgcc agaattttgt gtcccttctt catattttac
     3601 ttatcgtaac cgagacgaat caaaccaaag gcggcgtaat tcaacatatc aaggtaattg
     3661 gagtcgatcc cttcggaagc gatggtttgg ccgcctaaat cctcaatttg ctttaccctg
     3721 tttattttca ttaagatcag gtcggtatat gaactaatcc gcatacctct ccaggcttct
     3781 tcgtaatcaa aatttttatt gagcatcaat tgcctggcca tttccatttt ttcgtcgtac
     3841 agggccagcg cttcttccgg agtgatatct accttttccg aaaaaccttg ttgcaactga
     3901 ataaggccga caatggcata atttataata cccataaatt cgtcggcaat attatcctgt
     3961 attttgctct cgcctttgat ttcgatatta cgaatgcgat tggccttgat aaaaagttga
     4021 tcggtaacgg aaggtatgcg caaaatgcgc catgcggctc cgtaatcgtg cagctttttt
     4081 gagaaaattc tgcgacattt agcggttacg gcatcgaatt gttcattggt tttactcata
     4141 aagtgaggta tttcggatga atcacaaaat tactgatttt tagaaataaa aaccaacttt
     4201 tcgggccggt ctgaataaaa gacttgtaaa aaaattaagg ctttaaaacc gtatgacttc
     4261 ctattatgac ctgcccgata ggcggtgcca ttttaaaaac cagaccccgt ccggcatatt
     4321 tttccataca atcaaacacc agagaatact ccagctctgc cgtacaaaaa acaagtcccc
     4381 tgatgtcctt gtctttcaaa gcctcctcaa tttgatcttc gttggtaacc tgaataaaat
     4441 tcagggatcc ggtggacacg gtactgtaca aatctcgtac tctttcgaat tcggaggtgc
     4501 cgccccaaac aagaacagaa gcttgttgcg ttgaaaaagc gatttttttt attatgtaaa
     4561 gacattttcg caatagaccg gagaaaaaag aaaaggctcc gctcaaccaa atggcaagat
     4621 tgatgagcag ggcgtaaaaa aagtttttac ccaaaaaatg ttttttggca aaaatagcca
     4681 tcgcattgta aaaaacatat acatagttca gactgccctt tttagtgctt tcgcccttgt
     4741 aatgaagtat gcggccggga gggaaataat aattccggta gccggctttc aaaatgcgat
     4801 aggaccaatc gatgtcttcg ccgtacataa aaaaattttc gtccagcaaa ccggctttaa
     4861 taattgcttc tctgcggact aacatgcaag caccagccaa aatctcgacc gaatgaattt
     4921 tatctttatc cagccaaccc agatgataac gtccaaagcg ccgggaacgg ggaaacaagg
     4981 cagacaaacc ggaaatctta taaaaagaaa ccgccggcgt tggcaaacct cttttggatt
     5041 ccgccaaaaa acggcctcgt ccgtcaatca tcttgactcc cagagcgccg gcctccggat
     5101 gtgcatccat aaaggacagc atattacgaa tggcctcttc ctgcaccaag gtatcgggat
     5161 ttagcaaaag cacatagtcg gattcggagg cttcgatggc cagattgttg gctttggcaa
     5221 aacccaggtt ttcgctgttg gcaatcaact ttatttcggg atacgattgc cggagcattt
     5281 ccagcgaatc gtctgtggag cagttatcga cgacaaaaac ttctgtttct gtgtttttta
     5341 ttgctctata caaagaatgc aggcattgtt ccaaaaaata cctgacgttg tagtttacta
     5401 tgattaccga taacttcact tcattactaa ctgatagctc cccaatttat tttcttgccg
     5461 tagcgttttt ttaactcctc ggccatcaaa gcattttcgg gcaaagataa gtccggatct
     5521 ttctcgaggc attccccggc gatttggcgg gcatattcca atatctgggc atctttgccc
     5581 agatcggcaa ttttcaggtc gaagggaacc ccactttgct gcaggccttc caagtcgccc
     5641 ggcccacgca ttttcaggtc agcttcggct atctcgaaac cgtcgttact agaaaccata
     5701 atctctatgc gtttacgccc ctcgtcagac agcttgtacg aagtcaacaa cacacaatag
     5761 gactgatcgg ccccgcgtcc caccctgcct ctcaactggt gcaattgcga caaaccgaaa
     5821 cgttc
//
LOCUS       DAOSGL010000115         5816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12151_length_5816_cov_5.117688, whole genome shotgun sequence.
ACCESSION   DAOSGL010000115 DAOSGL010000000
VERSION     DAOSGL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12151_length_5816_cov_5.117688"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKY68_04765"
     CDS             complement(<1..743)
                     /locus_tag="PKY68_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24383.1"
                     /translation="MENDINQVQVNTMKISHKLIFLCLLITCCVLSSCQSEKQSDLRA
                     RMQEWDDILWEKSDIEIDEGPQAILDSLESINFKSLNYKNRAYYYLLQTIARDKSYYV
                     FENDSVISIAVKWYKNKPDAYNYSRSLFYHAIVLSQIHTNDSLMFFSIKTAENVFLEK
                     KLNDSLLYGRICVFMGSLHYSRGNYDISNDYYEKAASTFKAIGNTDRYISAKVNQVWY
                     LISIGEHDAALNMLNNIDYPEKISDKALS"
     gene            complement(728..1789)
                     /gene="thiL"
                     /locus_tag="PKY68_04770"
     CDS             complement(728..1789)
                     /gene="thiL"
                     /locus_tag="PKY68_04770"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOH24384.1"
                     /translation="MDGLDDITRPVERERTEIAKLGEFGLIEHLTAGNTLNQATTVLG
                     PGDDAAVLHYGEDKDILLSTDLLLEGIHFDLSYVPLKHLGYKAAVVNFSDICAMNGIP
                     SQLTVSVGISKRFSVEDMEELYSGIKIACKGYGVDLIGGDTSASLTGLCLCLTCTGEV
                     AKGQAVLRSGAKVNDLICVSGNLGAAYMGLQLLEREKLVYEGQQDFQPDFAGKEYLLQ
                     RQLRPEARKDIIEKLAQKKIRPTAMIDVSDGLSSDLLHICKQSNCGCRIFEDKLPIDY
                     QTASLAEEMNMNLSTAALHGGEDYELLFTVPLELHETIENWSDVRIIGYICHEQDGRK
                     LITRDNAEFDIIAQGWKMT"
     gene            complement(1792..2604)
                     /locus_tag="PKY68_04775"
     CDS             complement(1792..2604)
                     /locus_tag="PKY68_04775"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOH24385.1"
                     /translation="MYETIQKTLQWLEANTKLRPSIGIILGSGLGNLVDQIASPQRIP
                     YADIPNFPVSTVEGHSGNLVLGMLGGKAVMAMQGRFHYYEGYSMKELSFPLRVMKAFG
                     IKTLFLSNAAGGLNPTFKVGDIMVITDHLNLFPEHPLRGKNDPRLGTRFPDMGQPYSK
                     ILIERATVVAKKLNIPLKQGVYAGWQGPSFETPAEYRMLHLLGADACGMSTVPEVIVA
                     RHAGMDVFGLSIITNSASSASTEKASHEEVQEAAIKAQPLMTTLITELIKTL"
     gene            complement(2567..3670)
                     /gene="lpxK"
                     /locus_tag="PKY68_04780"
     CDS             complement(2567..3670)
                     /gene="lpxK"
                     /locus_tag="PKY68_04780"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOH24386.1"
                     /translation="MPSKCPARPLLLPLSPIFGVIVRFRNLLYDRGICKTSAFEIPTI
                     CVGNISVGGTGKTPHTEYLTKLLQKQYKLAVLSRGYKRKTKGFVLASKDSRVEDIGDE
                     ALQMASKFPEITVAVHENRVKGMQLLLKARPEIQVVLLDDAFQHRRLKAGLSIVLTDY
                     GRPVYDDFPLPAGNLRETPQALSRADIVICSKCPPDISAERRKQIASRLALKKGQNLF
                     FSGIRYGKLRAGFPDKGAYSAQSSLAELKQKDCKLMCFCGIASPNSFIGWVRQSFPEA
                     PALIFPDHHEYKASDLEKIYSAFERLENGNKCIITTQKDHMHLKNNPLFEALTPYIYY
                     IEIDIHFVDGQENVFKKLITDYVRNHTKNAAMA"
     gene            complement(3682..5445)
                     /gene="sppA"
                     /locus_tag="PKY68_04785"
     CDS             complement(3682..5445)
                     /gene="sppA"
                     /locus_tag="PKY68_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOH24387.1"
                     /translation="MKQFLKFSLASALGVVIALVVLSLIWLLVLLGLASSTESTPKLQ
                     NNTLLKIELKGVLSDYAQEDPFSEVANELFGMDQAAKIGLDEILAAIKKAKNNENISG
                     IYIESSSLQCGIASAQEIRKALSEFKESGKFIYAYANELFDQKEYYICSVADSLFLDP
                     NGILHFAGMSATPVFLTEMLDKIGIEPEIFKVGTYKSAVEPLINKEMSKENREQTLSY
                     LSGMWNNMLQEISADRNISVDKLNQLADRFMSLQPTKETVENGLIDDLFYPLEMMQFF
                     ADKLDSVKVKDLKMISVRALNKIPEKQTEFIKEKVAVLYAQGGISTEAPDGISSKKLV
                     REIDKLRENESVKAVVLRVNSPGGSAFASEQIWYALGELKKEKPLVVSMGDYAASGGY
                     YISCNADKIIAAPTTLTGSIGIFGLFFNVRELTEKIGLDFDMVKTNQHADFGDFSRKM
                     TPAEKVLMQNSVERGYDLFVERCAQGRGMENHQIRLIGEGRVWTGEQALERKLVDELG
                     YLDQAIDAAVELAELEKYRVVTYPEKKDFMTRVLESMNKNVSTRIIKNYLSEYFPGLQ
                     ELTETKLEPGILARMPYNLII"
BASE COUNT         1473 a         1291 c         1315 g         1737 t
ORIGIN      
        1 tacgataggg ctttatcgga tattttttcg ggataatcta tgttattcag catgtttaga
       61 gcggcatcgt gttcaccgat ggatatgaga taccatacct gatttacttt ggcggaaata
      121 tatcggtcgg tgttacctat ggctttaaaa gtggaggcag ccttttcgta gtagtcattt
      181 gaaatatcgt aatttcctct ggaataatgt aagctgccca taaaaacaca gattcggccg
      241 taaagcaaag aatcgttcaa tttcttctcc aaaaacacgt tttcggccgt tttgatcgag
      301 aaaaacatca gagaatcgtt ggtatgaatc tgagaaagca caatggcgtg gtaaaacaga
      361 ctccggctgt aattgtacgc gtcaggtttg tttttatacc atttcaccgc aatagaaata
      421 acagaatcgt tttcaaatac gtaatacgat ttgtccctgg caatggtttg caacaaataa
      481 taatatgccc tgtttttgta attcaggctt ttaaagttaa tgctttcaag gctgtcaagg
      541 atagcttgag ggccttcgtc aatttcgatg tcgctttttt cccacaagat atcgtcccat
      601 tcttgcatac gggctcgtaa atccgactgc ttttcgcttt ggcaggaact cagcacgcaa
      661 caagttataa gcaggcaaag gaaaatgagt ttgtgcgata ttttcatagt attgacttgt
      721 acctggttta tgtcattttc catccctgag cgatgatatc aaattctgcg ttgtcgcgtg
      781 taataagttt acggccatct tgttcgtgac atatgtaacc aataatcctc acatccgacc
      841 agttttctat tgtttcgtgc aactccaggg gcacagtaaa taaaagctca tagtcttcgc
      901 cgccgtgcaa agctgcagta ctcaggttca tattcatttc ttcggccagg gaggctgttt
      961 ggtaatctat gggtaatttg tcttcgaaaa tgcgacaacc acagtttgat tgtttgcaaa
     1021 tatgcagtaa atcggacgag agaccgtccg aaacatcaat catggcggtg gggcggattt
     1081 ttttctgagc cagtttctcg atgatgtcct tacgggcttc cggacgcagt tgtcgttgca
     1141 aaaggtactc tttaccggca aaatcgggtt gaaaatcttg ttgaccctcg tagaccaatt
     1201 tttcgcgttc caacagttgc agccccatat aggctgctcc caaattgcca gaaacacaga
     1261 tcaggtcgtt caccttagct ccgctacgca acacggcctg acctttagca acctctccgg
     1321 tacaggtcag gcaaagacac agtcccgtga gcgatgccga ggtgtcgcca ccaattaaat
     1381 cgacaccata tcctttgcag gctattttaa taccgctata gagttcttcc atgtcttcga
     1441 cagagaagcg tttggatatt cctacagaaa cggtcagttg agaagggata ccattcatgg
     1501 cgcaaatatc ggagaagttt accacggcag ctttatatcc caggtgtttc agaggcacat
     1561 aactcaggtc gaaatggatg ccttcgagta aaaggtcggt gctgagcagt atatccttgt
     1621 cttcgccata atgcagtacg gcagcatcgt cgccgggtcc caatacggtt gtagcttggt
     1681 ttagtgtgtt tcctgccgtc aggtgttcaa tgagtccaaa ttctccgagc ttggctattt
     1741 cggtgcgttc cctttcgaca ggtctggtaa tgtcgtccaa tccatccatg tttacagggt
     1801 tttaatcagt tctgttatca atgtggtcat caggggctga gcttttattg ctgcttcctg
     1861 aacttcctcg tgagaagcct tttccgtgct agccgacgaa gcggaattgg taatgattga
     1921 aagcccgaaa acatccatgc cggcgtgcct ggcaacgatg acttcgggga cggtggacat
     1981 tccgcaggca tcggctccaa gcaggtgtag catgcggtac tcggccggag tttcgaaact
     2041 tgggccttgc cagccggcgt acactccctg cttcaagggt atatttagtt ttttggccac
     2101 gacagtggct ctttctatca aaatttttga atagggctgc cccatgtcag ggaaacgggt
     2161 gcccagacgg ggatcgttct ttcctcttaa ggggtgttca gggaaaagat tcaggtggtc
     2221 ggtaatcacc atgatgtccc ctactttaaa agtgggattc aggcctccgg cggcattcga
     2281 tagaaacaag gttttaatgc cgaaagcttt catcacgcgc aggggaaaac tgagttcttt
     2341 cattgaataa ccttcgtaat aatgaaaacg cccctgcatg gccataacgg cttttcctcc
     2401 caacatgccc agtaccagat taccgctgtg tccttctacg gtggaaacgg gaaaattcgg
     2461 tatatctgcg tagggtatgc gttggggaga cgctatttga tcaaccaggt tcccgagccc
     2521 cgatcccaga attattccta tggaaggtct taacttcgtg ttggcttcaa gccattgcag
     2581 cgttttttgt atggtttcgt acataatcgg ttattagttt tttaaagaca ttttcctgtc
     2641 catcgacaaa atgaatatct atttctatgt aatatatata aggtgtcaat gcttcaaaga
     2701 gcggattgtt ttttaaatgc atatggtcct tttgggtagt aataatgcat ttgtttccgt
     2761 tttcaaggcg ttcaaaagcg ctgtaaattt tttcaagatc ggaggctttg tattcgtgat
     2821 gatcgggaaa aatcagagcg ggagcctccg gaaaggattg ccttacccat cctataaaac
     2881 tgttaggcga agctataccg caaaagcaca tcagtttaca gtctttttgt ttgagttccg
     2941 ccagactgct ttgggcagag taggcccctt tatcgggaaa gcctgcccgg agttttccat
     3001 agcggatacc cgaaaagaac aggttctgtc cctttttaag tgctaacctt gaggcaattt
     3061 gttttcttct ttctgccgaa atatcgggcg ggcatttact gcagataaca atatcggctc
     3121 tcgacaaggc ctggggagtt tctctcaaat taccggcggg caacggaaaa tcgtcataca
     3181 caggccgccc gtagtctgtc agcacaatag aaagtcctgc tttcaacctt cggtgctgaa
     3241 aggcgtcgtc gagcaatact acttggattt cgggtcttgc cttgagcaac aactgcatgc
     3301 cttttaccct gttttcgtga accgccactg tgatttcggg gaatttactt gccatttgca
     3361 gcgcttcgtc tccgatatcc tctactctgc tgtccttgct tgccagtaca aaacctttgg
     3421 tttttcgttt gtatcctctg ctcagtactg ccagcttgta ttgtttttgc aacagcttag
     3481 tcaaatattc ggtatgaggt gttttaccgg tgccgcccac gctgatgttg cctacgcaga
     3541 tggtggggat ttcgaatgcg ctggttttgc aaatacccct atcgtagagt aaattccgaa
     3601 aacgcactat cactccaaaa atcggggata atggcaacaa aagtggtcgt gccgggcatt
     3661 ttgaaggcat agacgatttt attagataat caggttatag ggcatccttg ccaaaatacc
     3721 cggttctaat tttgtttctg tcaattcctg caagccgggg aaatattccg ataagtaatt
     3781 ttttattatc cgtgtgctga catttttatt catgctttcc agtaccctgg tcataaagtc
     3841 tttcttttcc ggataggtaa ctaccctgta cttctcgagt tcagctaatt caacggctgc
     3901 gtcaatagct tgatcaagat aacccaattc atctaccagc tttctttcta aagcctgttc
     3961 tcctgtccag acccggcctt caccaatcaa acggatttgg tggttttcca tgccgcggcc
     4021 ttgtgcgcaa cgttcaacaa aaagatcgta ccccctttcg acgctgttct gcatcaatac
     4081 tttttcggct ggagtcattt ttctcgaaaa atcgccaaag tcggcgtgtt gattggtttt
     4141 caccatatcg aaatccagcc cgatcttttc ggtgagttcg cgaacattga aaaataggcc
     4201 gaaaatacca atggagcctg taagggtggt aggagcggca attattttgt cggcgttgca
     4261 cgagatgtaa taaccgcccg aagcagcata gtcgcccatg gaaacgacca ggggtttttc
     4321 ttttttcaac tcgccgaggg cataccagat ttgttcggaa gcaaaagcgc tgccaccggg
     4381 agagttcacc cgcaacacta ctgctttgac actttcgttt tcgcgaagtt tgtcaatttc
     4441 gcgaaccagt tttttgctgc tgatgccgtc gggagcttca gtgcttattc cgccctgagc
     4501 gtagagtaca gcaacttttt ctttgataaa ttcggtttgt ttttccggaa ttttatttaa
     4561 agctctgacg gagatcattt tgaggtcttt aactttgacg gaatcgagtt tatcggcaaa
     4621 aaattgcatc atctccaggg gataaaacaa atcgtcaatc agaccatttt caactgtttc
     4681 cttggttggt tgcagcgaca taaaacgatc ggccagttgg tttagtttat caacggatat
     4741 gttgcgatct gcggaaattt cctgcagcat attgttccac attccgctga ggtaagacaa
     4801 ggtctgttct cggttttcct tgctcatttc cttattaata aggggctcga cggccgactt
     4861 atatgtgcct accttgaaga tttcgggttc tatgccgatt ttgtcaagca tttcggtcaa
     4921 aaatacggga gtagcactca tgcccgcaaa atgaagaata ccattaggat ctaagaagag
     4981 ggaatcggct acgctgcaga tataatactc tttctgatca aagagctcgt tggcataggc
     5041 atagataaat ttgccgcttt ctttgaattc actcaaggcc tttctgattt cctgcgcact
     5101 ggctataccg cattgaaggc tgctgctttc gatatatatg ccgctgatgt tctcgttgtt
     5161 tttagccttt ttgatggccg caagtatttc gtccagccca atcttggctg cctgatccat
     5221 tccaaaaagt tcattggcga cttcgctaaa ggggtcttcc tgggcgtaat cgctcaggac
     5281 tcctttgagt tcgattttaa gcagggtgtt gttctgcaac ttgggcgtag attctgtcga
     5341 agaggcaagc cccaacaaca ccagcaacca gatcagtgac agcaccacaa gggcaatgac
     5401 aactcccaga gccgaagcca gggaaaattt taaaaactgt ttcatagtga taccattagc
     5461 ttgtattgta aatagccgtt tctgtctaag acgagtcgtg tttttcgtct caagacgaat
     5521 agtcacaaaa gtactaaaat aacccagggt ttaacaaaca aaaaaagcga tttattttag
     5581 cttagccggc atactttcgt ttttcaagcg ttttttttag acttgaaaat cagagcaatg
     5641 aaaaggattc aaaaaaaata ttgcaaaaac aagcagaata atttggtggg aaagaatcgg
     5701 tgtattatct ttgcacccgc ttttgatgga ggataccgtt gtgatggtag acaaaaatca
     5761 aaaacacatt tcgttctttg actgattgaa tatagtagta caagagagag cgggca
//
LOCUS       DAOSGL010000116         5809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12166_length_5809_cov_4.769552, whole genome shotgun sequence.
ACCESSION   DAOSGL010000116 DAOSGL010000000
VERSION     DAOSGL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12166_length_5809_cov_4.769552"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PKY68_04790"
     CDS             complement(<1..579)
                     /locus_tag="PKY68_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOH24388.1"
                     /translation="MRSWNILLLALLLPVFACQDKKEDKTEEESMDMTRQQLTNNYVD
                     TLILKKSTFSKQVNCNGKLRAVAKSELTMPGSGVLHTISVHNGSVVKKGDLLAVSDKE
                     DAELELRKARQDLKLAEIELLDNLIGQGYSQDMSDVPEVVLERAKIKSGYLSAQERLE
                     TARRRLDKCFLYAPFTGRVANMNAKLYQRADVF"
     gene            complement(587..2092)
                     /locus_tag="PKY68_04795"
     CDS             complement(587..2092)
                     /locus_tag="PKY68_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH24389.1"
                     /translation="MKKKPIHLIWLVLTGLWGISGIGYAQADKTFLSLERAILVAKER
                     SPWAQMARFTLMRQYWNHRSYKAELLPSLNLSGNALNYNRSIVDVRDAGTGQISYVSN
                     NSLANTVLLSIDQNIPFLGGTLSLESYLSHLEQFDYDKTTFNTNPLALSYRQPLRTYN
                     SLKWDMKTAPLVYEQAKRAYLESMQTIAIQTTSLFFSALSAQSNYEKSLATYNDRKDL
                     FNNAQNRFELLGTVMRSEILQLELSLLNAEMSVNTSKLNMETQLFNLCLYLGMTSHTR
                     VELVQPSEVPQLSISADLVLDKALTNSTHALKQNLKLQQSQRALAQAKSAKGLQATLS
                     AHLGFSQTGDRIGEAYQNLREREVVGISFRLPIYDWEMSKGKVKMAQADLAVTQTEIE
                     QADLEFRQDITTKVVRFNNQLRQCEISARAKSIAEERYKISKNRFENGMLTVTELNTA
                     QNEYDAALSQYINQLQVFWSAYYELQKIALYDFIHKIDLSADFNKIVDNLK"
     gene            complement(2579..3622)
                     /locus_tag="PKY68_04800"
     CDS             complement(2579..3622)
                     /locus_tag="PKY68_04800"
                     /inference="COORDINATES: protein motif:HMM:NF027202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BF3164 family lipoprotein"
                     /protein_id="HOH24390.1"
                     /translation="MKINYKEIFLLVIIIMCYSCEKQKSINYFDNTEHITESELFFTD
                     KKAILGRVFNIDVYDSLVILQHNLDEYAFSFIHSVNGELLAKWGSKGHGPNEYVQILG
                     DFDVYRGKLVFLDNTKHINFVPIAEVIHQQTNFNIDREPYPYIADFRPMRIVLMNDSI
                     KIALGGFEKGRFGIFSSHRILNCPYDYPFNYKEIQGIYRGSVFQSRMKTNQLENRFVL
                     SMLSSDIFEIYQLTDTTVERIFISPYKHIPTIWKKGNRYTINYARSVAGITGMAVTEN
                     YICFTYSSQSYEEAVQSSLYSNEILFYDWNGNKVKKVILPFSIGRFCLDDNYIYGLRT
                     EGEETCIYRFKMN"
     gene            complement(3718..3957)
                     /locus_tag="PKY68_04805"
     CDS             complement(3718..3957)
                     /locus_tag="PKY68_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24391.1"
                     /translation="MKKTIILSIAFVCIALGISLSSYLAMRENISSMAIANAEALTGV
                     EQPDVADCISDPDFNCEALHPTDPSKDKLRLRARW"
     gene            complement(4444..5322)
                     /gene="lepB"
                     /locus_tag="PKY68_04810"
     CDS             complement(4444..5322)
                     /gene="lepB"
                     /locus_tag="PKY68_04810"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH24392.1"
                     /translation="MKDKISNLLFITSCVILIFAGIVILHTLRRIYVFDQFIIPTSSM
                     EPTLIPGDRIIVNKLIIGARIYKEFDFSPGAPLICKRTKGRRKVQVNDVVVFNHPYGN
                     QKRKIGFRINYVCAKRCIGTPGDTVSINNGYYLNKRYGDVIGNSGQQQAFSKITENIC
                     DSRAWKTSWLSYIDTTWNTHDMGPLYIPRKGDTIIMDRYHYLIYGYIIEYETGYRCRY
                     KNDSVRLEGHHLESYTFRHNYYYFCGDNIANSNDSRNWGFVPEDFIIGVADRISYSKD
                     QTTGRYIKNRFMKRIK"
     gene            complement(5312..>5809)
                     /locus_tag="PKY68_04815"
     CDS             complement(5312..>5809)
                     /locus_tag="PKY68_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HOH24393.1"
                     /translation="CRSWNKFRVFYQNEAYEEAVRAYEEYHADLKNNVKFLFEYGHSL
                     HKLKRYEESNTILMEAAQKSCDPMILNIIGKNHQGLEQYEKAEHYFIRSTHMIPGRMY
                     PYFLLVNLYAEPNNYKEDKLLEAARQVLEFEPKVPSTAINEMRLEVKKILVANKSKQS
                     PSNER"
BASE COUNT         1654 a         1193 c         1075 g         1887 t
ORIGIN      
        1 aaacacatcg gcacgttggt acaatttagc attcatattt gccacacgac cggtaaatgg
       61 agcgtacaaa aagcatttgt ccaatcttct ccgggctgtt tcgagccttt cctgagcaga
      121 aaggtaaccc gatttgatct tggctcgttc caacaccact tcaggcacat cagacatgtc
      181 ttgactgtac ccctgcccga tcaaattgtc taagagttcg atttctgcca gttttaggtc
      241 ttgacgagct tttcgcaatt ccagttcggc atcttccttg tcggaaactg ccagtaaatc
      301 gccctttttt accacactcc cgttgtgcac ggaaatggta tgcaacacac cgctcccggg
      361 catcgtcaac tcggatttgg ccaccgccct tagttttccg ttgcaattga cctgtttgct
      421 gaaagttgat tttttcagaa tcaaagtatc cacataatta ttagtcaatt gttgacgggt
      481 catatccatc gactcttcct ctgttttatc ctctttttta tcctggcatg caaatacagg
      541 caaaagcaaa gccaagagta aaatgttcca acttctcatc ctgtggttat ttaaggttat
      601 caactatctt attaaaatcc gcactcaggt ctattttatg aataaaatcg tacaatgcaa
      661 ttttttgcaa ttcgtaataa gccgaccaaa acacttgcaa ctgattgatg tactggctta
      721 aggcagcatc gtactcattc tgggcggtat tcagttcggt cacagtcaac atgccgttct
      781 cgaaacggtt tttgctgatc ttgtaacgct cttcggcgat acttttggcc cgagccgata
      841 tttcgcattg acgtaattga ttattaaagc gaaccacctt ggtggtgata tcctgccgaa
      901 attccaagtc ggcttgctct atttcggtct gtgtaactgc cagatcggcc tgagccattt
      961 taaccttgcc cttgctcatt tcccaatcgt agatgggcaa cctgaaagaa attccaacca
     1021 cttcccgttc gcgtaaattt tggtaagcct ctcctatcct gtctcctgtt tgcgaaaacc
     1081 ccagatgtgc actcaaagtt gcctgcaagc ccttggctga ttttgcttgc gccagggccc
     1141 gttgagattg ctgcaatttc aggttttgct tcagtgcatg agtggaattg gttaaggctt
     1201 tgtccaacac gagatcggca gaaatactca actgcggcac ttcggatggt tgcactaatt
     1261 caacccgggt atggctggtc atacccaggt aaagacatag attaaacaat tgcgtttcca
     1321 tattcaattt cgaagtattc acactcatct ccgcattcag taaagaaagc tccaattgga
     1381 gaatttcgct ccgcattact gttcccagca actcaaaccg attttgggca ttgttgaaca
     1441 aatctttgcg atcgttgtac gtagccagac ttttttcgta gttcgattga gccgataaag
     1501 ccgaaaaaaa caaagaagtg gtctgtatcg caatggtctg catggattcc aggtaagccc
     1561 ttttggcttg ctcgtaaacc aagggggccg ttttcatatc ccatttcaag gaattatagg
     1621 ttctgagagg ctgcctgtag ctcaaggcca aaggattggt attgaaagtc gttttgtcgt
     1681 aatcgaattg ttccaggtga gacaaatacg attccaaaga caaagtgcca cccaaaaaag
     1741 gaatattctg atctatcgaa agtaaaacag tattggccaa ggaattattg ctgacataag
     1801 agatttgccc ggttccggca tcgcgcacat ctacaatgga ccggttgtaa ttgagtgcgt
     1861 tgcccgacaa attcaacgaa ggaagtaatt ccgccttata cgaacggtga ttccaatatt
     1921 gcctcatcaa ggtaaaacga gccatttgcg cccagggcga tcgttctttt gctaccaaaa
     1981 tggctcgctc gagcgataaa aacgttttgt cagcctgcgc ataaccaatg cccgatatgc
     2041 cccagagtcc tgttaaaaca agccaaatca aatgaatagg tttttttttc atagttatat
     2101 tagcaaagtt ctggatgctg tttccagctt aaaaattata atatcgacag tatagttcaa
     2161 cggagatttt tgtttgtaac aacttttaaa gaagcatgat tctaaattat aatttcatat
     2221 agtatctgtt ttcgagtgta aagaaaaata aaataaacct agtgtggccg tcttttgttt
     2281 actttttttg aatacaattg actccatttt ctatttgatg cgcttcataa cggtagtatg
     2341 taaatcaagt ttatcatcat catattttct ctataaaaat ccagtaccct ttctaattcg
     2401 ctgcaattct tgccgctttg ccgcaaggcc tcctctactg gcccggagta ttggcacgac
     2461 agaaaaagaa ggatgccggt aaacagcata attgttgtga caatttgcat agaaggaaaa
     2521 tcttgacgat ctccatacgt attataactc atacatactt gaacgacgtt atggatgttt
     2581 aattcatttt aaatcgatag atgcacgtct cttcaccttc agtacgaagt ccgtatatat
     2641 agttatcatc caggcagaaa cgaccaattg aaaaaggtaa tatcactttt tttactttgt
     2701 ttccattcca atcgtaaaaa aggatttcat tcgaatataa agaagattgt accgcttcct
     2761 cataactttg tgacgagtag gtaaaacaga tgtaattttc tgttacagcc attccggtta
     2821 ttccagcaac acttcttgca taatttattg tataacgatt cccttttttc catatagtgg
     2881 ggatgtgttt gtatggacta ataaatattc gctctaccgt tgtgtctgtc aattgataga
     2941 tttcaaaaat atcagacgac aacatggaca gtacaaatct gttttctagt tgatttgttt
     3001 tcatcctgga ttgaaaaaca gaaccccggt agataccctg tatttcttta taattaaagg
     3061 gataatcata aggacaattt agaatccgat gactggagaa tataccaaaa cgaccttttt
     3121 cgaatccgcc aagtgcaatt tttatactgt cgttcataag gacaatgcgc atcggcctga
     3181 aatcagcaat gtagggataa ggctctctat caatattgaa gtttgtttgc tgatgaatga
     3241 cctcagcaat aggcacaaaa ttgatgtgtt ttgtattatc caaaaagaca agctttcccc
     3301 tgtagacatc aaaatctcct aagatttgga catattcgtt tggaccatgt cctttactac
     3361 cccatttggc taataattcc ccgttgacag aatgaataaa agagaaagca tattcatcaa
     3421 gattgtgttg taaaataaca aggctatcat atacatcgat attaaagaca cggcctaata
     3481 tggccttttt gtccgtaaaa aacaattcgc tttcagttat atgttcagta ttatcaaaat
     3541 agtttatact tttttgtttt tcacaagaat aacacattat aataattact aaaagaaata
     3601 tttctttata attaattttc atattcttta tcaaataacg aattatacat tgtcattaaa
     3661 acttttatga taagttggag gttgcctcca acttatcata aattgtttac ataattttca
     3721 ccaacgcgct ctaagcctaa gtttatcttt acttggatct gtcggatgaa gtgcttcaca
     3781 attgaaatct ggatccgata tacaatcagc aacatctggc tgttcaactc cagttaatgc
     3841 ttcagcattc gcaatagcca tgcttgaaat attttctctc atagctaaat acgatgacaa
     3901 acttatcccc aaagcaatgc aaacaaatgc tattgataaa ataattgttt ttttcatagt
     3961 taaactgatt taaagattta caaaattcaa aataaatgtc ctggtttaat caatagttat
     4021 gactgtatct atcctttcaa cagccctgaa ctacggaggt tcttttattt tgacttttcc
     4081 gctcactttc gctaaatttt caggtttctc cttagaatgt agctgcgctc ggcataatcc
     4141 aacaaaagtt ggcttctgct ctcactttcg ctacatttgt cacctccttt cttttttggg
     4201 agtacgtcgg gggagtaagg cctttgcagt ttgaacttgt gcggcgggct ctgctccctt
     4261 tctttcctca gggtttattt aaatttttca atctatagat tcctaatttt aatttgactt
     4321 agagtttttt tcgattgcga agaaagatag aataaattta atttaactac cttttatttg
     4381 ccttttttga atacaattga gaaaagagca atgggtaaac tgttgctttg tcctcccatt
     4441 tttttatttg attcgcttca taaaacgatt cttgatgtaa cggccggtgg tttggtcttt
     4501 ggaataggaa attcgatcag ctaccccaat tataaaatct tccggtacaa aaccccaatt
     4561 cctgctgtcg ttggagttgg caatgttgtc tccgcagaaa taataatagt tatgacggaa
     4621 agtataactt tccaagtgat gtccttccaa ccgaacagaa tcgtttttat aacggcaacg
     4681 atatcctgtc tcgtactcaa tgatataacc gtaaattaaa taatggtaac ggtccataat
     4741 aattgtgtcg cctttgcgag gtatatacaa aggtcccatg tcgtgagtgt tccatgttgt
     4801 atcaatgtag ctcagccagc ttgttttcca tgctcttgaa tcacatatgt tttctgtaat
     4861 ttttgaaaag gcttgttgct gaccgctatt tccaataaca tccccatacc ttttgtttaa
     4921 ataataacca ttgtttatgc tcaccgtgtc cccgggagtc cctatacagc gtttagcaca
     4981 gacgtaattt attcgaaaac caatcttgcg tttttgattg ccgtagggat gattaaagac
     5041 gactacatcg tttacctgaa ccttgcgcct tccttttgta cgtttgcaga tcaacggagc
     5101 acccggagaa aaatcaaatt ccttgtagat gcgcgcccca ataattaatt tgttgactat
     5161 gattctgtca ccaggaatca aggtgggttc catcgaagaa gtgggtataa tgaattgatc
     5221 gaaaacatag atacgtcgta atgtatggag aatgactatt cccgcaaata tcaatataac
     5281 acaagaagtt ataaataata aattcgatat tttatctttc atttgagggc gattgttttg
     5341 atttattagc aacaagaatc ttcttcacct ccagtcgcat ttcgttgatg gctgtgctag
     5401 gcactttggg ttcaaattcc aacacctgac gggctgcctc aaggagctta tcttccttgt
     5461 agttatttgg ttcggcgtat agatttacaa gtaggaaata ggggtacata cggccgggaa
     5521 tcatatgtgt tgaacggata aaataatgtt cggctttttc gtattgctct aatccttgat
     5581 ggtttttgcc gatgatatta agtatcattg gatcgcaact tttttgagcc gcttccataa
     5641 gaatagtatt ggattcttcg taccgtttca atttatgcaa gctgtggccg tattcaaaaa
     5701 gaaatttaac gttgttcttt agatcggcat ggtattcttc ataggccctg acagcctctt
     5761 cataggcctc gttttgatag aaaacacgaa atttgttcca ggaacggca
//
LOCUS       DAOSGL010000117         5787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12235_length_5787_cov_3.457781, whole genome shotgun sequence.
ACCESSION   DAOSGL010000117 DAOSGL010000000
VERSION     DAOSGL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12235_length_5787_cov_3.457781"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="PKY68_04820"
     CDS             <1..1212
                     /locus_tag="PKY68_04820"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HOH24394.1"
                     /translation="KKIYIPKEFREMDFNDPASRYSYHRMAYSDNIVVFWEAGFGPDL
                     GNPPELNGKDMRVDLENVLNKAESFYRYFRDTLHFLKGESKADVYRMMLMINYSEDGT
                     AYGGAYDNEIGALWVTPFRLKDPKCNVIAHELGHSFQAQIGADGHLNLKGGPIWEMTS
                     QWMLFNVNPHWMTDENYHLVDYLKKTHLAFMHPQNMYHSPYVLEFWSDKHGPEIIASI
                     WNDAVKGEDAVTAYKRLSGIDQETFNAEMFEAAQKFMTWDFDRIRTYAKPYANMHYSK
                     IKQDAEGWYAIAEENCPQHYGYNGIRLDVPQPGKNIRLDFEGLAPEEQAGGWRYGLVA
                     VDKEGKVTYGNMQTKTKGKFRYRVPKDGLSHLWLVVSTAPEQHSPDTKTTWPYRFSIQ
                     GGQVAKEQLMQ"
     gene            1384..4854
                     /locus_tag="PKY68_04825"
     CDS             1384..4854
                     /locus_tag="PKY68_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24395.1"
                     /translation="MSPKNPLNIKTKKTGKWAIGGSFALLMLLSINSCIDTRYDISKG
                     ISTKIAFGGDSLSIPLGSTDTIRVGDLLNADSIEVISIGEDGGYGIKMSDSIYVEVPG
                     IDKSTLVLEDQHFKPEPIFVSFGDISLKDFSIPGIEQTSNLNLNMNEISLEDFVLPVI
                     DETDRYGSGMTEYALDPEALEVNIEPVEAITPALLKDGYLTVLSETDPPIEVPLLLPN
                     DTIGISTQSELSFSFDVPDGVSNIDTVELKKYPEKAVLKVELKLDGAVTKTDTIFTKG
                     SIVPNVIIDPSRLFVFGAGTPLNDEGKIVFGVEETLSNENKFSAEKEMYIDAFKIEDE
                     PVGGSIGPAETIRVTGHVVVDTLFLYSDKLNTAQNLNLLVEISINGVVIESMTFDLPE
                     LKTQISGSSPFILNDEMEEEVNKVNIVHLENPGSITVEISGENFPEMNSRSILIDSFV
                     IYFPEEFILEPTPGLSDNKYTITNEPFDSYVGKKIIMNLRGLNMKDIALVKDGDVQSI
                     HWEDSISFKGSVSINGRIDSKNIPAEGNDAGMKVQISSDLNFDYAEITTNEITDALVP
                     AYINLSFDIDIAKEVKRLDTINMVENTFIHLNLDMPELPLDVEGDIKVEFPPLFSFKP
                     ALPNNELLIQGIIPETIDLELASLNINRTLSDGTLDLVDSIRISGDVKMLPGLVSSKD
                     TEGLSSKTMAVRTTSDELKITSSSLHLNTLQANFSDSTLIEIQTIDLPKEILSLDSIV
                     LEEGAILELTANITNMPELSSPLMLDFALKFPDLFVFMPGTVDAQNTWSFEEDFSKRE
                     IKKALYIKGLKFDGKSLGETLDINEMIRYNADVSVVDPSVISDDLTGDSISVGIEVKL
                     SNVNFKSVYGKVDPGIKPEPQLIPIEGLPDFLKDTSIVLDINPVLSIQTASNLGIPVA
                     TSIRLIPVINDVDQLGKAQDISLNLTRSATAADTARNYFWIATDSAGMPDNYQLLQTN
                     IKDLFSTIPEKLKIEIQAETDLSVQHEVDLDADYFMNVHYDVSVPLSFGKDFNLTIRD
                     TINDLDPSIGEMAFAGGGLELYGSISNSIPMELDIQIIPINEFNETIPIEAVTQKIHS
                     GKVDGSATDTDLSLKILDPDGLLKDLSGFILIFTASSNETVAGTPIKPENFVKATLKA
                     RLFGGIIIGE"
     gene            4873..>5787
                     /locus_tag="PKY68_04830"
     CDS             4873..>5787
                     /locus_tag="PKY68_04830"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOH24396.1"
                     /translation="MKTSFLKIGTFVLLGSLMLTSAKAQQATSRTAYFMENATQSHQM
                     NPALSPYNGYVSIPMLGSLGLGLESNMAFTHFIYPPLTEGGQLMTFMHPDVDAQRFLS
                     KLRPNNYLRAGLQANLLSFGFYTEENFWTFDLATKLGIGMNIPYEFFAFAKNGMTSGS
                     GNEYHIRDLSVNAGAYVEASAGHSRQLFDFLRVGMKLKGLIGIAQVKANISEMDIVMS
                     PSRWSIQTRGSLEGYARGAAFTNDSTGAVNGIDFKTPGIGGFGMAVDLGAVYNTPIEN
                     LTASLGIIDLGGISWGKKNTLRAKSQGSV"
BASE COUNT         1725 a         1306 c         1248 g         1508 t
ORIGIN      
        1 aagaaaatct atattcccaa agaattcagg gagatggatt ttaacgatcc ggccagtcgt
       61 tacagttatc atcgtatggc atactccgat aatatagtgg tcttttggga agccgggttc
      121 gggccggatc tgggtaatcc tccggaatta aacgggaaag acatgcgggt agacttggaa
      181 aacgtactca ataaagctga aagcttttat cgttatttcc gcgacacttt gcatttctta
      241 aaaggggaat ccaaggcaga cgtctatcgc atgatgctta tgataaacta ttccgaagac
      301 ggcacggcct acggaggcgc ctacgacaat gagataggtg ccctctgggt gactcctttt
      361 cgcctgaaag accccaaatg taacgttata gcccatgaac tcgggcattc ctttcaagcc
      421 cagatcggtg ccgacgggca tttaaacctc aaaggcggac ccatttggga aatgacctca
      481 caatggatgc ttttcaatgt caatcctcac tggatgacag acgaaaatta ccatttagtc
      541 gactacctca agaaaaccca tctagccttt atgcatcccc aaaacatgta tcattctcct
      601 tacgtgttgg agttctggtc cgacaaacac gggcctgaaa taattgcttc catctggaac
      661 gatgccgtaa aaggagaaga tgccgttact gcctataagc gtctgagcgg tatcgatcag
      721 gaaacattta acgcagagat gtttgaggct gcacaaaaat tcatgacctg ggatttcgac
      781 cggatacgca catacgccaa accttatgca aatatgcatt acagcaaaat caagcaggat
      841 gccgaaggct ggtacgccat tgccgaagaa aactgtccgc aacattacgg ttacaacggc
      901 attcgcctag atgttcccca acccggaaag aacatccgtc tcgatttcga aggccttgcc
      961 ccagaagaac aagccggagg ctggcgttac ggcctggttg ctgtggataa agaggggaaa
     1021 gttacttacg gcaacatgca aaccaaaacc aaggggaaat tccggtatcg tgtgcctaaa
     1081 gacggattaa gccatctatg gctggttgtc agcacggctc ccgaacaaca ttcgccggat
     1141 actaaaacca cctggcccta tcgcttcagt attcagggcg gacaggtggc caaagaacaa
     1201 cttatgcaat aatccttgtt tttggttaaa attaattaca aacaaaggct gctctttacg
     1261 tagagcagcc ttttttttgc aaaaaacgcg catttttatt tcaaaattat ataatatggg
     1321 tatctttgaa gattcaaaaa aaggattccc ctagtttatc ctccttataa ttaattttct
     1381 gtgatgagcc caaagaaccc cctgaatata aaaacaaaaa aaaccggcaa atgggccata
     1441 ggtggttcat ttgctctatt aatgctgcta agtatcaact cctgcatcga cacccgatac
     1501 gatatcagca aaggcatttc gaccaaaata gcctttggcg gagactcctt gtccatacct
     1561 ttgggcagta ccgacaccat ccgggttgga gatttactca atgccgattc cattgaagtg
     1621 atttctatag gtgaagacgg aggctacggc atcaaaatga gcgattccat ctatgttgag
     1681 gttcccggta ttgataaatc gactttagtg ctcgaagatc aacacttcaa accggaaccc
     1741 atcttcgtta gttttggaga cattagtctg aaggacttca gcatccccgg cattgaacaa
     1801 acttccaatt taaatcttaa tatgaacgag atatcgttgg aagattttgt acttccagtc
     1861 atcgacgaaa ccgacagata cggttcaggt atgaccgaat atgctctgga ccccgaagcc
     1921 ttagaggtaa acatcgaacc cgtggaagct attacacctg ctttattgaa ggatggatac
     1981 ctgacagttc tgtccgaaac agatccaccc atcgaggttc ctttactgtt gccaaacgac
     2041 accattggta tttctaccca aagtgaactc agtttttcat tcgatgtccc tgatggtgta
     2101 agtaatatcg ataccgttga attgaaaaag tacccggaaa aagccgtatt aaaagtagaa
     2161 ttgaaattag acggagccgt aacaaaaacc gatacgattt ttaccaaggg aagtatcgta
     2221 ccgaacgtta tcatcgaccc cagccggctt ttcgtatttg gcgcaggtac acctctcaac
     2281 gacgagggta aaattgtttt tggtgtcgaa gaaactctaa gtaatgaaaa taagttctcc
     2341 gccgagaaag aaatgtacat cgatgctttt aaaattgaag acgagcctgt cggtggcagt
     2401 attggcccgg ctgaaaccat cagggttacc ggacatgtgg tcgtggacac cttgtttttg
     2461 tactcagaca aattaaacac ggcccaaaat ctgaaccttc ttgtagaaat ctccataaac
     2521 ggcgttgtga tcgaatctat gacatttgat ctacctgaac tcaaaacaca gatttccggc
     2581 agcagtccgt ttatactgaa tgacgaaatg gaggaggagg tcaacaaggt taatatagtg
     2641 catcttgaaa atccaggaag tattactgta gagattagcg gtgagaactt cccggagatg
     2701 aatagccgaa gtattttgat tgattctttt gttatctatt ttcccgaaga atttattctt
     2761 gagcccacgc ccggactcag cgataataaa tacactatta caaatgagcc gtttgattcc
     2821 tacgtaggca aaaaaataat catgaatctt cgcggactca acatgaaaga tattgccctt
     2881 gtgaaggatg gggatgtgca aagcatccat tgggaagatt ccatcagctt caaaggatcg
     2941 gttagcataa atggaagaat cgacagcaaa aatattcctg ccgaaggtaa cgatgccggc
     3001 atgaaagttc agatcagttc cgatttaaac tttgattatg ccgaaataac caccaacgaa
     3061 ataacagatg ctttggtacc ggcttacatt aacctctctt tcgacatcga cattgccaaa
     3121 gaagttaaac gtctggatac catcaacatg gtagaaaaca cttttattca tttaaatttg
     3181 gatatgcccg aactgccgct ggacgtcgaa ggagatatca aagttgaatt tcctcctctc
     3241 ttcagtttta aaccggcatt acccaacaac gaacttctga tacagggaat aattcctgaa
     3301 acgatagatc tggaattggc ctcattgaat ataaatcgga ctctgagcga cgggaccttg
     3361 gatttggtcg attccatacg gattagcggt gacgtgaaaa tgctgccggg attggtcagc
     3421 agcaaagaca ccgagggcct atcgagcaaa accatggctg tcagaaccac cagcgacgaa
     3481 cttaaaatca cttcctcttc tttgcatttg aataccctgc aagctaactt ttccgattca
     3541 accctgattg agatacaaac aatagacctg cccaaggaaa tactctcact ggacagcatt
     3601 gttttggaag aaggagccat tcttgaactc actgcaaata tcacgaacat gccggaatta
     3661 tccagtcctt tgatgcttga ttttgctttg aaattccccg acttgttcgt gtttatgccc
     3721 ggaacggttg atgcccaaaa tacttggtct tttgaagaag atttttctaa aagagaaatc
     3781 aaaaaagcgc tttatatcaa aggtcttaaa ttcgacggta agagcttagg tgaaacactc
     3841 gacatcaacg aaatgatcag gtataatgcc gatgtttcag tggtggatcc ttccgtaata
     3901 agcgacgatc tgacgggaga ttcaataagt gtgggtatag aagtaaaatt gagcaatgta
     3961 aactttaagt ctgtttacgg caaagtcgat cctgggatta aacctgaacc acagttaata
     4021 cccatcgaag gcttacccga tttcctcaaa gataccagca ttgtactcga catcaatcct
     4081 gtactttcca tacagacggc aagtaacctg ggtattcctg ttgctacaag catcaggctt
     4141 attcctgtta tcaatgacgt cgatcaatta ggaaaagctc aggacataag cttaaacctg
     4201 accagatcgg ccacagccgc cgatactgcg aggaattatt tctggattgc gacagacagc
     4261 gccggaatgc ccgataatta tcaactcctg caaacaaaca taaaagacct tttcagcact
     4321 attcccgaaa agctcaaaat agagattcaa gcggaaacag atctctcggt tcaacacgaa
     4381 gttgatctgg acgccgatta ctttatgaat gtacattacg atgtgtctgt acccctgagt
     4441 tttggcaaag atttcaactt aaccatacgc gataccatca acgatctgga tcccagcatc
     4501 ggcgaaatgg cctttgccgg cggcgggctc gaattgtacg gcagcatttc gaattccatt
     4561 cccatggagc tggatattca aataattccc ataaatgagt tcaatgaaac tatcccgatc
     4621 gaagccgtta cccaaaaaat tcattccggc aaagtggacg gaagcgccac cgataccgat
     4681 ttatcgctaa aaatactgga tcccgatggt ctcctcaaag atttgtcagg ctttattctt
     4741 atattcacgg caagttccaa cgaaacagtg gcaggaacgc ctatcaaacc ggagaatttt
     4801 gtaaaagcca cgctcaaggc ccgcttattt ggtggaatta tcataggtga ataatctaaa
     4861 aaaatatatc taatgaaaac ttctttctta aaaatcggca cattcgtatt gctcggcagc
     4921 ctgatgctga cttcggcaaa agcacaacaa gccacttcca gaacagccta ttttatggaa
     4981 aatgccaccc aaagccatca gatgaatcct gcattgagcc cctacaacgg ctatgtcagc
     5041 ataccaatgc tgggatctct tggtttgggt ctggaatcta atatggcttt tactcatttt
     5101 atctatccgc ctttaacgga gggggggcaa ttgatgacct ttatgcaccc cgatgtcgat
     5161 gcccagcgct ttctgtcgaa actcagaccc aacaactatc tgagggcagg cttacaagcc
     5221 aacctcctct cctttggttt ctacacggaa gagaatttct ggacctttga tttggctacc
     5281 aaactcggaa ttggaatgaa tatcccctat gagttttttg ctttcgccaa aaacggcatg
     5341 acctcgggtt ccggcaacga gtaccatatt cgtgatcttt ctgtgaatgc cggagcttat
     5401 gtggaagctt ccgccggaca ttccagacaa ctcttcgact ttttgagggt cggtatgaaa
     5461 ctaaaaggat tgatagggat cgcacaggta aaagccaaca ttagcgagat ggatatcgtt
     5521 atgagcccct cccggtggtc tattcaaacc agaggcagtc tggagggtta tgccagagga
     5581 gctgctttta ccaacgattc taccggtgcc gttaatggta tagatttcaa gacaccgggg
     5641 ataggtggct ttggtatggc tgtcgatttg ggtgccgttt ataacacccc cattgagaac
     5701 ctgacagctt ctctgggtat tatcgactta ggtggtatat cctggggtaa aaaaaatact
     5761 ttgcgcgcca aatcgcaggg cagcgta
//
LOCUS       DAOSGL010000118         5777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12266_length_5777_cov_3.726844, whole genome shotgun sequence.
ACCESSION   DAOSGL010000118 DAOSGL010000000
VERSION     DAOSGL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12266_length_5777_cov_3.726844"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PKY68_04835"
     CDS             <1..1408
                     /locus_tag="PKY68_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HOH24397.1"
                     /translation="ACNTDFNGYYFESLTDIPSSDTYNEKYKDYEENPFIKVSEQPVS
                     TFSVDADGGSYANMRRFLHLGQTPPKESVRIEEFINYFTFDYKEPASDENVSLESEIS
                     TCPWNTEHHLLRLGMKGMTIPADKLPNSNYVFLIDVSGSMDSPDKLGILKTGFKTLVD
                     NLRDQDKIAIVTYAGQAAVLLKSTYGHEKRKIKAAIDKLGAGGSTAGAAGIITAYEIA
                     EKNFIQNGNNRVILGSDGDFNVGPSSTEELIELIEEKRESGIYLTVLGVGGGNLNDHM
                     MEQIANKGNGNYEYIDNAKQIEKVFTHEILKFYTVAKDSKIQITFNPTMVDSYRLIGY
                     ENRKLNDEDFENDTTDAGEIGSSQTITALYELVLLDVPDTEKYAQFDFRYKKPNETQS
                     RLIAHEINTTPKEMRFSSENMRFAASVAGLGLLMKQSEYKGTLTKQMVLDLGREAKSF
                     DPNGYRKEFIELVTDWNN"
     gene            1704..3323
                     /locus_tag="PKY68_04840"
     CDS             1704..3323
                     /locus_tag="PKY68_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOH24398.1"
                     /translation="MKNYLKISLLLTPFLFLFSCTPKEKMETGRFEPTWESLSQYEVP
                     QWFRDAKFGIWAHWGPQCQPEAGDWYARNMYYQGHWQYRYHVEHYGHPSEVGFKDVIN
                     QWKAENWNPEELLALYKRAGAKYFFALGNHHDNLDLWNSKYQPWNSVAVGPQKDILAG
                     WAQAARNNGLPFGVSIHSAHAWRWYEGAQLSDKEGPLAGVPYDGKLTKKDGKGKWWQG
                     LDPQDLYAQNHELSEESENIGSIHNQWHWGNGALAPSEEYCLNFYNRTVDMINQIRPD
                     LIYFDDTGLPLWPVSNVGLEVAAHYYNSNMRLNEGKLEAVLFGKVLTEEQKACMVWDV
                     ERGAPDQIQELPWQSCTCIGGWHYERGIYNRNGYKSAKTVVQLLVDIVSKNGNMLLSI
                     PLRGDGSIDDKALKIVEDIAQWMDINSECIYETRPWHIYGEGPKAEQANPINAQGFNE
                     GRGGPYTADDIRFTRKGESLYAVIMEKPAPKQRILIKSLAADSPYFETKFDKVDLLGA
                     ESVTYNFGKTGLEIRLPDIQDLPAPLVLKIY"
     gene            complement(3423..5516)
                     /locus_tag="PKY68_04845"
     CDS             complement(3423..5516)
                     /locus_tag="PKY68_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOH24399.1"
                     /translation="MAKTELETKAEAKVEAEAASIEAEAETKVEADVVAKTELETKAE
                     AKVEAETASIEAEAKVETKAETKVEAEVEAEVEAEVEAEAKVETEAKVEAKAPKEEEA
                     PKEEEAPAKTETAPGAASLIDHSVIRNLIDQLRELIQKESIDVKSRIENIKQSFYKQL
                     KLDIEASRNAFIKAGGDPADFVPSEVNAIEAEFKELMNQWREKRIKLTADLEKQKKDN
                     LIKKNQLIEVIKALTDSSDDIRQKIPEFRKLQQEWKNIGQVPAEDVNELWKNYQFEVE
                     RFYDQIKITNEFRDYDFKKNLEIKTELCEIAERLKEDSDVVKAFKRLQKLHEEWRETG
                     PVAKELREELWQRFKDASTEINKKYQAFFERLKEIENENLAQKTAICESLEAIDYSKL
                     KSFRDWDRKTTEVIEWQERWKTIGFAPKKANAKVFERFRTACDLFFQTKSNYYKSVKE
                     SLSQNLELKRELCEKAESLKDSTDWNATSNQLIQIQKEWKTIGPVPRKHSDALWKRFI
                     SACDYFFEQKEKNTSSQRSVEYQNLAAKKELIAQVKALAESEESTDKENEETLRTIRE
                     LNAKWNAIGFVPFREKDKIYKQWRNAMDSLMSHFNIDKASRRLSSFQSNLEDIKGKGQ
                     SKVLHERERLMRQFESITNEIKTCENNIGFFNTTKASTGSALLKEMNRNIEKLREERD
                     LIYKKIQIIDGELQA"
     assembly_gap    5534..5633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1357 a         1255 c         1405 g         1660 t
ORIGIN      
        1 tgcttgtaat acagacttta atggatacta ctttgagtct ttgacggata ttccgtcgtc
       61 ggacacttac aacgaaaagt acaaggatta tgaggagaat ccctttatta aagtgtcgga
      121 gcagccggtt tctacctttt cggtggatgc cgatggcggt tcttatgcca atatgagaag
      181 gtttcttcat ttggggcaga ctccgcccaa ggaatcggtg aggatagaag agtttatcaa
      241 ttatttcact ttcgattata aggagccggc ctcggacgaa aatgtttctt tggaatccga
      301 aatttccact tgtccctgga atacggagca tcacttgctg aggctgggta tgaaaggaat
      361 gactataccg gccgataagc tgcccaattc caattacgtg tttttaattg acgtttcggg
      421 atcgatggat tctcccgaca aattgggaat attaaaaacc ggttttaaaa ccctcgtcga
      481 taatttaaga gaccaggaca aaattgccat tgtcacctac gccggtcaag cggctgtttt
      541 actgaaatca acctatggac acgaaaaacg gaagattaaa gcggccatcg ataaattggg
      601 tgcgggaggt tccaccgcgg gagcggcagg tattataacc gcctacgaaa tagctgaaaa
      661 gaattttatt cagaacggca acaacagggt gattttgggc agcgacggcg actttaatgt
      721 tgggccttcg tcgacggaag aattgattga attaatcgaa gaaaaacgcg aaagtgggat
      781 atacctgacc gttcttgggg taggaggagg caacctcaac gaccatatga tggagcaaat
      841 cgccaacaaa ggcaacggca attacgaata catcgataat gccaaacaaa tcgagaaagt
      901 gttcactcat gaaattttaa aattttacac cgtagccaaa gattcaaaga tacagatcac
      961 cttcaatcca accatggtcg actcctacag gttgatcggc tacgaaaacc ggaaattaaa
     1021 cgatgaagac tttgaaaacg atacgaccga tgccggtgag atcggttcgt cgcagacaat
     1081 aacggcattg tatgagcttg tattgctcga tgtacccgat acggaaaaat acgctcaatt
     1141 cgatttcagg tataaaaaac cgaacgaaac tcaaagcaga cttattgctc acgagatcaa
     1201 tacgactcca aaggagatga gattttcgtc cgaaaatatg agatttgccg catcggtggc
     1261 cggcttaggc ttgttgatga aacaatccga atacaaagga acgctcacca aacaaatggt
     1321 attggattta ggaagagagg ccaaatcttt cgatccgaac ggctacagaa aagaattcat
     1381 agaattggtg accgattgga ataattaaaa aacctcgcca aatcttgcat acttcgtgca
     1441 gccgactgtt gcgcacaagg atgattgcgt atcttcctgt tgttaaaggc gacagtcgga
     1501 tcaacagcta ccggtgcgtg cgttgtattc gcaaattgtt ggccggcgtc gaagcacaaa
     1561 gttcagattc agctaccggc acctgtgtcg tccgtcctgg tacagtcgaa ttatttatgt
     1621 aaattagcgg attgaaaaac cggagagtat catagaactc ttacatgaac taattcgtaa
     1681 taaccagtta aactatgacc aaaatgaaaa actacttaaa aatcagcctg ttgctaaccc
     1741 cttttttgtt tcttttttct tgcacaccca aagaaaaaat ggagacaggc cggtttgagc
     1801 ccacttggga atcgttgagc cagtacgaag tgccgcaatg gttcagagat gccaaattcg
     1861 gtatctgggc gcattgggga cctcagtgtc agccggaagc cggcgactgg tacgccagga
     1921 acatgtatta ccagggtcac tggcaataca ggtatcatgt ggaacactac ggacatccct
     1981 ccgaagtggg attcaaagat gtgatcaacc agtggaaggc cgaaaactgg aaccccgagg
     2041 aattgcttgc tttatacaag cgcgccgggg ctaagtactt ttttgccttg ggcaatcacc
     2101 acgacaatct cgatttgtgg aacagtaaat accagccctg gaattctgtg gctgtaggtc
     2161 ctcaaaaaga cattttggcg ggttgggccc aagcggccag aaacaatggt ctgcctttcg
     2221 gcgtgagtat tcattcggcc cacgcctggc gatggtatga aggagcccag ttatccgaca
     2281 aggaagggcc gctggccggt gttccttacg acggcaagct gaccaaaaaa gacggcaaag
     2341 ggaaatggtg gcaaggcctg gatcctcagg acttgtatgc gcaaaaccac gaactgagcg
     2401 aagagtcgga aaacataggc agcatccaca atcaatggca ttggggcaac ggcgctttgg
     2461 cgccttcgga ggagtattgc ctgaatttct acaatcgcac ggtggacatg atcaatcaga
     2521 tccggcccga cttaatttat ttcgacgaca ccggtttgcc cttgtggccg gtcagcaatg
     2581 tgggattgga ggtggccgcg cattactata attcaaatat gcggctaaac gagggcaagc
     2641 ttgaggccgt tttgtttggt aaagtgctca ccgaggaaca aaaagcctgc atggtatggg
     2701 acgtcgagcg gggagctccc gatcaaattc aggaactgcc ctggcaatct tgtacttgca
     2761 tcggaggctg gcattacgaa cggggcatct acaacagaaa cggctataaa tcggccaaaa
     2821 ctgtggtgca gcttttggtg gacatagtga gtaaaaacgg aaatatgttg cttagtattc
     2881 ctcttcgcgg agacggctcc atagacgata aagccttgaa aattgtagaa gacatcgctc
     2941 aatggatgga tatcaacagc gagtgcattt acgaaacccg tccttggcat atatacggcg
     3001 aaggccccaa agctgaacag gccaatccta ttaatgctca aggctttaac gagggtaggg
     3061 gcgggcctta caccgcagac gatattcgtt ttacccgcaa aggggagtcg ctttatgctg
     3121 taataatgga gaaacccgct cccaagcagc gtattctgat caagtcgctg gctgccgaca
     3181 gcccctattt tgagacgaaa tttgataagg tcgatctgct gggcgccgag tctgttactt
     3241 acaatttcgg taaaacaggt ttggagatca gactgcccga tattcaggat ctgccggcac
     3301 ctttagtgct gaaaatctat tgatagggcc gattcttaca gtaagtcctt ggtgtctgtt
     3361 ttaaatttat agcccggtga ctttcgtcgc cgggctataa atgtttgaac tattgagaag
     3421 ttttaggcct gcagttcgcc gtcgatgatc tgtatctttt tgtagataag gtcgcgttct
     3481 tctctgagtt tttcgatgtt gcggttcatt tcctttagca gggcagaacc cgtggaagct
     3541 ttggtggtgt tgaaaaaccc gatgttgttt tcgcaggtct tgatttcgtt ggtgatcgat
     3601 tcgaactgac gcatcaggcg ttcgcgttcg tgcagcacct tggattgccc tttgcctttg
     3661 atgtcttcca aattactttg gaagctgctc aggcgccggc tggccttgtc tatgttgaaa
     3721 tggctcatca ggctgtccat ggcgttgcgc cattgtttgt agattttgtc tttttccctg
     3781 aaaggcacaa aaccgatggc attccacttg gcgtttaatt ccctgatcgt gcgcaaggtt
     3841 tcttcgtttt ccttgtcggt gctttcttcc gattcggcca aagcttttac ctgggcaatc
     3901 agttcctttt tagctgccag attttggtat tccacgctgc gctgactgga ggtgtttttt
     3961 tctttttgtt caaagaaata atcgcaagcc gagatgaagc gtttccacaa agcatcggaa
     4021 tgttttctgg gtaccggacc tatggtcttc cattctttct gaatctgaat cagttggttc
     4081 gaggtggcat tccagtcggt gctgtctttg aggctttcgg ctttttcgca gagttctctc
     4141 ttcagttcca ggttttggct gagtgattct ttaaccgatt tgtagtagtt gcttttggtc
     4201 tggaaaaaca ggtcgcaggc agtgcggaaa cgttcgaaga ccttggcgtt ggcttttttg
     4261 ggagcaaaac ctatggtttt ccagcgttcc tgccattcga tgacttcggt ggttttgcgg
     4321 tcccagtcgc ggaagctttt caatttgctg taatcgatgg cttccaggct ttcgcaaatg
     4381 gcagttttct gagccaggtt ttcgttttct atttctttga gcctttcgaa aaaggcctgg
     4441 tattttttgt tgatttcggt agaagcgtcc ttgaagcgtt gccaaagttc ttcgcgcaat
     4501 tcttttgcga cagggccggt ttcgcgccat tcttcgtgca gtttctgcag gcgtttgaat
     4561 gctttgacta catcgctgtc ttcttttaac cgttcggcta tctcgcagag ttcggtttta
     4621 atttcgaggt tctttttgaa atcgtaatcg cggaattcgt tggtgatctt gatctggtcg
     4681 taaaaacgtt caacttcgaa ttgataattt ttccagagtt cgtttacgtc ttcggcggga
     4741 acttgcccga tgtttttcca ttcttgctgg agtttccgga attcggggat tttctggcga
     4801 atatcgtcgg agctgtcggt aagggccttg atcacttcga tgagctgatt tttcttgatc
     4861 aggttgtctt ttttctgttt ttcaaggtcg gccgttaatt tgatacgttt ttcgcgccac
     4921 tgattcatca gttctttgaa ctcggcctcg atggcattga cttcgctcgg aacgaaatcg
     4981 gcaggatcgc cgccggcctt gataaaggca tttctggagg cctcaatatc cagcttaagt
     5041 tgtttgtaaa aggattgttt tatgttctct atcctgctct ttacgtcgat actttctttt
     5101 tgtatgagtt ctctgagctg gtctattaaa tttctgatga cggaatggtc aataagggaa
     5161 gcggctccgg gggcagtttc ggttttggcc ggagcttctt cttctttcgg agcttcttct
     5221 tctttcggag cttttgcttc gactttggct tcagtttcga ccttagcttc agcttcgact
     5281 tcagcttcga cttcagcttc gacttcagct tcgaccttgg tttccgcttt agtttctact
     5341 tttgcttctg cctcgatgct ggctgtctca gcttcaacct ttgcctcggc ttttgtttca
     5401 agttcggttt tggccacaac atcggcttcg accttggttt ctgcttctgc ttcgatgctg
     5461 gctgcctcag cctcaacttt tgcctcggct tttgtttcaa gttcggtttt ggccacaaca
     5521 tcggcttcga cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntggtttc
     5641 tgcttctgct tcgatgctgg ctgcctcagc ctcaaccttt gcctcggctt ttgtttcaag
     5701 ttcggtttcg accaaagctc cggcttcagc ctcagtttta gtcttggctt tgggagtttt
     5761 agcctttggc gttttta
//
LOCUS       DAOSGL010000119         5772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12276_length_5772_cov_4.350359, whole genome shotgun sequence.
ACCESSION   DAOSGL010000119 DAOSGL010000000
VERSION     DAOSGL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12276_length_5772_cov_4.350359"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PKY68_04850"
     CDS             complement(<1..878)
                     /locus_tag="PKY68_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH24400.1"
                     /translation="MRKKFQLPTIPLLLQGSLLLIFCTNYLQTQAGQTDVSFLQSLVN
                     DSQMLLARAVEGQEEGNYLSGSKQDFQTALEPALALLNDSTAAQADIDQQTDQLHQAY
                     LDFLDKRLGITDPWQADNYMHLAASTAQWAHYNVHDPTVVRSRGYFYEYGTDAAWGYS
                     VKGIPYRRSRDLVNWEYRGTVFDAYPQEVLNWKHSVDPNSNRTMTGIWAPYILKVGSE
                     FRLYYSAIFSDAGRAMIVLATSNSPEGPWTNRGWIVHSTNTTVFNAIDPTVTIGKDNS
                     HWMLWGSWTQGIYAIEL"
     gene            971..1831
                     /locus_tag="PKY68_04855"
     CDS             971..1831
                     /locus_tag="PKY68_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH24401.1"
                     /translation="MSKSKEVIIVTGASAGIGKAIVQSLAGCGIRLIMACRNMEKSAA
                     VKAILEQDNPGAEIELLNLDLASFASIRQFASTLARKKYRINTLINNAGVLPGRFSLT
                     RDGFETTLGVNYLGLFLLNELLLPLMRPGSLIINTISCSYIIGRLNKDMFIPNPGARF
                     MSIFSYARSKRAILVYSLELAERLSGQGIRVLATDPGIVSTKLIAMKRWFDPLTDALF
                     RPLIKTPGQGADTAVFLTLEAQKPASENAHLSGLCFKNRKAMRLSAKIRDKKIRKELW
                     DWTTAAIQGR"
     gene            complement(1839..2768)
                     /locus_tag="PKY68_04860"
     CDS             complement(1839..2768)
                     /locus_tag="PKY68_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536715.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOH24402.1"
                     /translation="MHKLYYSFPEFIGQYFKGKIQKISLDAGFSCPNRDGSLGRGGCS
                     YCNNLSFNPSYSSTKRSISEQLAEGKEFFAHKYPKMDYLAYFQSYTNTYDKLDRLIDL
                     YEEALRFPGVKGLVIATRPDCLPDDLLDYLEQLAKRCFLLVELGLESTLDRTLKEVGR
                     GHDYACSVNALNKIAQRRILCGAHIILGLPGESKEDMLAHAGRLNTLPLTTLKLHQLQ
                     LIRGTRLEEQVRNNPAYVHFFDAGSYARLLIDFIERLRPGLVLDRFVSQAPAEWLIGP
                     DWGLKNYEFTHQLEKMMRAGGHYQGRLYSGSNN"
     gene            2863..5061
                     /locus_tag="PKY68_04865"
     CDS             2863..5061
                     /locus_tag="PKY68_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOH24403.1"
                     /translation="MRTFRVPLILLCLIFQLSTVKAAGIELDSIIAGAYRPEIPSGWQ
                     SMNEGSSYACLAQGGKLILRYDYKSGKLLDTLLNVEKTRNVRMEKIEGFEFNPQESMI
                     LVWKNKKPIWRRSFTAEYFVYDRLHNILDSLSAVAPVREASFSPDGRMVVFSRNNNLF
                     IKKLDFKTEVAVSTDGKTNEIINGTADWVYEEEFMTTRMYEWTADSKFLCFVRFDERA
                     VPEHFFSMYGASKPEYPEYKYHPGEKRFRYPRSGENNSKVSLHCYNVQYRTLQKIELP
                     VDEETYIPRLRRMENPDLVAVMTLNREQNLFRMFTANPKSLLSRLILSESSDTYYDPS
                     YLDDILFTDKDFTYVSERDGYRHLYLYGVNGAFIRQLTSGPNDLTNYYGRDLRKNYFY
                     YQAVDDLPYRRSLYRVDARGRTTKLSDKDGINQAVFDKHFNYCIWTWSDRNTPPVSVL
                     CQSDNAKVISTLKDNAALRQKLSALSLPQKEFFETPAADGQKLYGWMLKPAGFDPSLR
                     YPLVMLQYSGPDSQDVLDSFGIGWEYYLAARGIIVVSVDGRGTGGRGEQFRKQTWLKL
                     GQYEVQDQIAAARALGTLPYVDAARIGIWGWSYGAYISLLALTEGTQYFKAGIAVAPV
                     TDYRYYNTAYTERFMRTPQQNKENYDKTSVLQRASSLKGELLLIHGLADDNVHANQTM
                     ELIDALVQEGKQFQMQLYPNRNHSILGLSNRKHLYTRMSDFFIQTLNADK"
     gene            5058..>5772
                     /locus_tag="PKY68_04870"
     CDS             5058..>5772
                     /locus_tag="PKY68_04870"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOH24404.1"
                     /translation="MKEGYLRKPEWLKIRLGDSEQYGITKQIIERHRLHTICTSGRCP
                     NQGECWSRGTATFMILGEICTRSCKFCNTLTGKPLPPDINEPEKVAESIRLMKLRYAV
                     LTSVDRDDLPDLGCSHWVAVVNKVREYNPGIVVETLIPDFQGRRELIAEIARLAPEVV
                     SHNLETVARLTPLIRSAAKYRRSLDVLEQLGAEGITTKSGLMLGLGETEEELMKTMDD
                     LLAVNCRILTLGQYLQPTSR"
BASE COUNT         1485 a         1394 c         1427 g         1466 t
ORIGIN      
        1 agctcgatag cgtaaatgcc ttgagtccag gaaccccaca gcatccagtg gctgttgtcc
       61 tttccgatgg tcaccgtagg gtcgatggcg ttgaacaccg tcgtattggt ggaatgaaca
      121 atccatcccc ggttggtcca gggaccttcg ggactattgg aagtggccag cacgatcatg
      181 gctctgccgg catcgctgaa aatggcggaa taatagagcc ggaattcgct gcccactttc
      241 aggatataag gagcccagat gccggtcatg gttctgttgc tgttgggatc gacgctgtgt
      301 ttccaattga gtacttcctg aggataggca tcgaagacgg tgccccggta ttcccagttt
      361 accagatcgc ggctgcgccg gtagggtatg ccttttacac tgtaacccca ggctgcgtcg
      421 gttccgtatt cgtagaaata acctctgctg cggactaccg tgggatcgtg gacgttgtaa
      481 tgagcccatt gggcagtgga cgcagccagg tgcatatagt tgtcggcctg ccaggggtct
      541 gtgatgccta agcgtttatc cagaaaatcg agataagcct gatgaagttg gtctgtttgt
      601 tgatcaatgt cagcctgagc ggccgtgctg tcgttcaata aggcaagggc aggctcgagg
      661 gcggtttgaa aatcctgttt ggatcccgaa aggtaatttc cttcctcttg tccttctacg
      721 gcccgtgcca gcaacatctg actgtcgttg accagacttt gcagaaagct gacatcggtt
      781 tgcccggcct gggtttgaag gtagtttgta caaaaaatca gcaggaggct gccctgcagt
      841 agtaagggta ttgttgggag ttgaaacttc tttctcattg ttgggtaatt ttaaatgcaa
      901 agaaaataaa aattaagcag acagccttgc tttttgcttt ttgactatct ttgaaacctt
      961 taaaccgaaa atgtccaaat ctaaagaagt tatcatagtc accggagcct ctgccggaat
     1021 aggtaaggcc atagttcaga gtctggccgg ctgtggcata cgccttatta tggcttgtcg
     1081 taatatggaa aagtcggctg ctgttaaagc tatccttgaa caagacaatc ccggagccga
     1141 aatcgaactt cttaatctgg atctggcttc attcgcgtct atcaggcaat ttgcttctac
     1201 cctggctcgg aaaaagtacc gtatcaatac cctgatcaat aatgcgggcg tgctgccggg
     1261 gcgtttcagc cttacccgcg acggttttga gacaaccctt ggggtaaatt acctgggttt
     1321 gtttttgctc aatgaactgc ttttgccctt gatgcgtccc ggttcactta tcataaatac
     1381 catttcctgc tcttatatca tcggtcgttt gaacaaagac atgttcatac ccaatcccgg
     1441 tgcccggttt atgagcatat tcagctatgc ccgttccaag agggccattt tggtgtacag
     1501 tctggaattg gcagaacggc tttcagggca gggcatacgg gtgttggcaa cagacccggg
     1561 gatagtcagc actaaactga ttgcaatgaa gcgatggttc gaccctctga ccgatgcttt
     1621 gttccgcccc ctgatcaaaa cacccgggca gggagcggat acggctgttt ttctcaccct
     1681 ggaagcacaa aaacccgcat cggaaaacgc ccatctgagc ggcctttgtt ttaagaaccg
     1741 taaagcaatg aggctttctg caaaaatcag ggataaaaag atccgtaaag agctttggga
     1801 ttggacaacg gctgccattc aaggccgcta attctttatc aattgttcga accggagtag
     1861 agcctgcctt ggtaatgccc tccggcacgc atcatttttt ccagttggtg ggtaaattcg
     1921 taattcttca atccccagtc cgggccgatc agccattcgg cgggagcctg cgaaacaaaa
     1981 cgatccagga caagaccggg ccggaggcgc tcgataaaat cgatgagcag gcgcgcgtag
     2041 gaaccggcat cgaaaaaatg cacataggcc gggttgttcc tgacttgttc ttccagcctg
     2101 gttcctcgta tcaactgcaa ctgatgcagt ttaagagtgg ttaagggtaa agtattcagg
     2161 cgtccggcat gcgcgagcat atcctctttg ctttctcccg gcaagcccag tataatatgc
     2221 gctccgcata aaatccggcg ttgggctatt ttgttcaaag catttaccga gcaggcgtaa
     2281 tcgtgccccc ttcccacttc tttcagcgtt ctgtctaagg tggattccag cccgagttcc
     2341 accagcagaa agcagcgctt tgccagttgc tcaaggtagt cgagtaagtc atccggcaga
     2401 caatcaggcc tggtggctat caccaatccc ttcaccccag ggaaacgcaa ggcttcttcg
     2461 tataagtcta ttagtctgtc cagcttatcg taagtgttgg tatacgattg aaaataagcc
     2521 aaataatcca tctttgggta tttatgggca aagaattctt ttccctcggc gagttgttcg
     2581 cttatactgc gtttggtgct gctataagaa ggattaaagc tcagattatt gcaataactg
     2641 caacctcccc gtcccaggct accgtctcga ttgggacaac tgaaacccgc atccaaagag
     2701 attttttgaa tcttcccttt aaaatattgg ccgataaatt cagggaaact gtagtaaagt
     2761 ttgtgcataa ctcggcaaaa ttacaaactt aattaaagcc ttctgcaagt cggaacaatt
     2821 ttttaccttt gcccatcttt caatgtttca gacccttttc ttatgagaac ttttcgtgta
     2881 ccactgatat tgctatgcct gatatttcaa ctatctactg taaaagcagc cggcattgag
     2941 ttggattcta ttatagcggg ggcctaccgg ccggaaattc cctccggctg gcaatctatg
     3001 aacgaaggca gctcttacgc ctgcctggcg caagggggta aattaatact gcgttacgac
     3061 tacaaaagcg ggaaactgct tgatactttg ttgaatgtgg aaaaaacccg aaacgtacgg
     3121 atggaaaaaa tagaaggttt tgagttcaac ccccaggaaa gtatgattct ggtatggaaa
     3181 aacaaaaaac cgatctggcg gcgttcattt acggcagaat attttgtgta cgatcgcttg
     3241 cacaatattt tggattctct ttcggccgtg gctccggtta gagaggcctc tttttcgccg
     3301 gacggcagaa tggtggtttt ttccagaaac aacaacctgt tcataaaaaa actggatttc
     3361 aagactgaag tagcagtaag caccgacggg aaaaccaacg aaatcataaa cgggaccgcc
     3421 gattgggtgt atgaagagga attcatgacc acccgtatgt acgagtggac ggccgacagt
     3481 aaattccttt gttttgtccg ctttgacgaa agggcggtgc ccgagcattt ctttagtatg
     3541 tacggagcct caaagcccga atatcccgaa tataaatacc acccgggaga aaagcgattc
     3601 cggtatcccc gtagcggaga aaacaacagc aaagtgagtc tgcattgtta caatgtgcaa
     3661 taccgtactc tgcaaaagat agagctgccg gtggacgaag aaacctatat acctcgtttg
     3721 cgccgtatgg aaaatcccga tcttgtagct gttatgacgc tcaacaggga gcaaaactta
     3781 ttccgcatgt tcacggccaa tcccaaatcc ttgctcagcc gcctgatatt gtcggaaagc
     3841 agcgatacat actacgatcc ctcgtatttg gatgatatct tgttcacgga taaggatttt
     3901 acctatgtga gcgaaagaga cggatacaga catttgtatt tgtatggtgt caacggcgcg
     3961 tttatccgtc aattgacgtc aggtccgaac gacttaacca actattatgg gcgtgacctg
     4021 cggaagaatt atttttatta tcaggcggtg gacgaccttc cttacaggcg ttcgctctac
     4081 agggtggatg ccagaggcag aacaaccaaa ctcagtgaca aggatggcat caatcaggcc
     4141 gtttttgata aacacttcaa ctactgcatt tggacctggt ccgataggaa tactcctccg
     4201 gtgtcggttt tatgccagtc tgataacgcc aaggtaattt caacacttaa agacaatgcc
     4261 gctctcagac aaaaattgag tgctttgtcg ttgcctcaaa aagaattttt cgaaacgcct
     4321 gctgccgacg ggcaaaaact ctacggatgg atgctgaaac ccgccggttt tgatccctcc
     4381 cttcgatatc ccctggtgat gttacaatac agcggccccg acagtcagga tgtgctggat
     4441 tcctttggaa tcggctggga atattatctg gcagcccggg gaataatcgt ggtcagtgtg
     4501 gatggccgcg gaacaggggg caggggagag caattcagga aacaaacctg gctcaagctt
     4561 ggccagtacg aagtacagga tcagattgcc gctgcaaggg cgttgggtac attgccctac
     4621 gtggacgctg cccgcatcgg catttggggc tggagttacg gtgcttacat cagcttgttg
     4681 gctctgaccg aaggtactca atatttcaag gccggtatag cggttgctcc cgtcacggat
     4741 taccgttatt acaacacagc atatacggag cgatttatgc gtacccctca gcaaaacaaa
     4801 gagaattacg acaagacctc ggtattgcaa cgcgcctctt cccttaaagg agaattgctg
     4861 ctgatccacg gtctggccga cgacaacgtg cacgccaatc agaccatgga gctgatcgat
     4921 gctctggtac aggaaggcaa acagtttcag atgcaactct accccaaccg caatcacagc
     4981 atcctgggtt tatccaaccg caagcatctt tatacaagaa tgtccgactt ctttatacaa
     5041 actttgaacg ctgacaaatg aaggaaggct atttgcgcaa acccgaatgg ctcaagattc
     5101 gcctgggcga cagcgagcaa tacggtatca ccaaacaaat tatcgaacga catcgcctac
     5161 acaccatctg taccagcggt cgctgcccta accagggaga atgctggagt cggggaacag
     5221 ccacctttat gatcttgggg gaaatatgta ctcgttcctg taagttttgc aataccctga
     5281 caggcaaacc cctgcccccc gatatcaacg aacccgaaaa agtagccgaa tccattcgtt
     5341 tgatgaaatt aaggtacgcc gtactcacct cggtagatcg cgatgatctg cccgatttgg
     5401 gttgttccca ttgggtagct gtcgtgaaca aagtgagaga atacaatccc ggtatcgttg
     5461 ttgaaactct gattcccgat tttcaaggca ggagagagct gattgccgaa atagcccgcc
     5521 tggctcccga ggtggtgtcg cataatctgg aaaccgtcgc ccgtctgact cccctaatac
     5581 gttcagccgc caaataccgc cgcagtctgg atgtgctgga acaattgggc gcagagggaa
     5641 tcaccaccaa gtccggcctg atgttgggtt tgggtgaaac cgaagaagaa ttgatgaaga
     5701 ccatggacga ccttttggca gtgaattgcc gtatactcac tttagggcaa tatttgcaac
     5761 ccacctcccg gc
//
LOCUS       DAOSGL010000120         5771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12277_length_5771_cov_4.786389, whole genome shotgun sequence.
ACCESSION   DAOSGL010000120 DAOSGL010000000
VERSION     DAOSGL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12277_length_5771_cov_4.786389"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKY68_04875"
     CDS             complement(<1..307)
                     /locus_tag="PKY68_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOH24405.1"
                     /translation="MSSITNKQVLDFILYEKEEWANSALMKFKTTEVLPEIFPGQFVQ
                     VKIENSPDTYLRRPLSVNFTDRENSCLWLLVQKIGPGTRKLAGLALNSKVNMILPLGK
                     "
     gene            complement(392..835)
                     /locus_tag="PKY68_04880"
     CDS             complement(392..835)
                     /locus_tag="PKY68_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH24406.1"
                     /translation="MKDRIKEILDSQGLNATQFAQKVNINVSALSHILSGRNNPSYDA
                     LQKIIVNFPGINVEWLMTGQGEMYKENLPIQQEEVTLFGENPQKPDHDSGKEGYPPQK
                     GLKSSQKPSMYTDNQTNKVTEKITAKISKIAVFYSDNTYEEFFPQ"
     gene            1657..2358
                     /locus_tag="PKY68_04885"
     CDS             1657..2358
                     /locus_tag="PKY68_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOH24407.1"
                     /translation="MDIKSILDFLHLLSQNNNREWFNKHKAFYTTSREHHIRLLEKLI
                     LLLADFDAELRHLRPQECIFRIYRDLRFTQDKTPYKTFFGAFMAAGGKNSERAGYYLH
                     LKPDNCFITGGIWMPEAPQLKILRQSIVDNLDEFEDIVHSQDFVSSFGSLDGRRLRTA
                     PKGFPADFPGMDYLKFKDYTATHKLSALEMERSDFCEYIAGLFQHLYPLNRFLNFALD
                     EYKENKPFYNHPINS"
     gene            2361..2807
                     /locus_tag="PKY68_04890"
     CDS             2361..2807
                     /locus_tag="PKY68_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOH24408.1"
                     /translation="MILDSLKNSSLYESMHPLFKRAFDYLKSRDLDLLEPGKIVLDGD
                     KLYVSISEYEGKSPEAARLETHEKYIDIQVIISGTETMGWRAANDCKHALAPYDTVKD
                     ICFYKDNPVSFVEVHPGEFTVFFPGDGHAPCIGKGPIKKAVVKVLL"
     gene            2807..4804
                     /locus_tag="PKY68_04895"
     CDS             2807..4804
                     /locus_tag="PKY68_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOH24409.1"
                     /translation="MKSKTLKIIKNDPWLEPYRQAIEGRYNYALSREKSLTKGGSLSE
                     FANGHLFFGLHKHPDGWTFREWAPNADSIYLIGEFNQWERRPEYALQKKEHGVWEIHL
                     AADCLKHGDLYKMYVSWPGGSGERIPAWTRRVVQDPVSLIFSAQVWEPANPYRFKHKV
                     KPPSQPLFIYECHIGMATEEERIGTYDEFRRLVLPRIVDNGYNAIQIMAIQEHPYYGS
                     FGYHVSSFFAASSRFGTPEELKRLIDEAHGYGLAVIMDIVHSHAVKNSVEGLGCLDGD
                     PNLYFHSGDRREHPAWDSLCFNYGRDEVLHFLLSNCKYWLDEFRFDGFRFDGVTSMLY
                     RSHGLGESFSSYDDYYNLNQDGDAIAYLTLANKLIHEIKPKAITIAEEVSGMPGLAAS
                     IKDGGIGFDYRMAMGIPDFWIKLIKEYSDEDWKPGAIWWELTNRRKDEKTISYAESHD
                     QALVGDKTIIFRLIDADMYWHMSKNDQNLVVDRGIALHKIIRLLTASTINGGYLNFMG
                     NEFGHPEWIDFPREGNGWSYKYARRQWSLLENRQLKYADLHAFDKAMVMLLKKINPKE
                     PLYQRWLHDQDQIMAFERASFLFVFNLHPSRSFVDYGILVQKGNYKIVLNSDAKEFGG
                     FGLVNEQIVHTTTQTAKNPDFELEWIRLYLPSRTALVLKRQ"
     gene            complement(4885..5502)
                     /locus_tag="PKY68_04900"
     CDS             complement(4885..5502)
                     /locus_tag="PKY68_04900"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOH24410.1"
                     /translation="MSPTIILANGEFPRKPGLLHLLRQAEHVVCCDGAAEKLLNFGRE
                     PSVIVGDMDSLSPSLKELYADRIITIDEQETNDLTKAVNYCERAGYRQIILMGATGIR
                     EDHTLGNISLLVDYSLRIEDICMQTNYGRIDVVRHYKQLQSYPGQQISLFAIDPSTIV
                     NAEGLKYPLIDRRLTNWWQGSLNEALGSTFEIRTQKGAVLVYRTD"
     gene            complement(5505..>5771)
                     /locus_tag="PKY68_04905"
     CDS             complement(5505..>5771)
                     /locus_tag="PKY68_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH24411.1"
                     /translation="QQFSYIQHEEFRLPAYTQFNFSAVYSGCLVGNSDFELGFYINNL
                     LGNQPLCNAWGYEIAFLSGEEPYRETGYYVLAPRHYMLKFVFKI"
BASE COUNT         1658 a         1267 c         1183 g         1663 t
ORIGIN      
        1 acttgcccag gggaagtatc atattcacct tggaattcaa agcaaggcct gctaatttac
       61 gggtccccgg accgattttt tgcaccaata accacagaca ggagttttct ctgtctgtga
      121 aattcaccga taagggtcgc cttaaatagg tgtcgggaga attttctatt ttaacctgta
      181 caaattgtcc cggaaagatt tccggcaaaa cttcggttgt tttaaacttc atcaaagccg
      241 aattggccca ttcttctttt tcgtataaga taaaatctaa aacttgcttg tttgtaatcg
      301 aagacataat taggagttga atacgcaaat atacgtgaga aatttcattt caggcctatc
      361 cgatttttaa actgtactcc accaagttta attattgtgg aaaaaattcc tcgtaggtgt
      421 tgtcggagta aaaaacggct atcttagata ttttcgcagt tattttttct gtgactttat
      481 ttgtctgatt atctgtatac atgctaggtt tttgtgagct ttttaagcct ttttgagggg
      541 gatatccttc tttacccgag tcatgatccg gtttttgggg attctcgcca aataaagtga
      601 cttcttcctg ctgtatgggc aagttttctt tatacatctc cccctggccg gtcatcaacc
      661 actcaacatt gattccggga aaattcacaa taatcttttg tagagcatcg tagcttggat
      721 tattccttcc gctcaaaata tgagataagg cagatacgtt tatattgacc ttttgcgcaa
      781 attgtgtagc attcaaacct tgagaatcta aaatttcctt aattctatct ttcataaact
      841 ggatatatat acatttttct gttgcacaaa tgtacaaata attgtctgga aaaatctttc
      901 caatcaaaaa tattttacag aaacaaatct gcctatagcc acattaataa aattattttt
      961 acgtgatttc tgttatgtaa agaccgtttt acatatttgt ttttacaata ctatattaat
     1021 taattatgag cctattgctc taaatgaaga atcttactcc tttataccca gcattataat
     1081 gatcttattt taaatctatg cttttaattt atctgctcaa agtcgttttt tgcccttatt
     1141 atgtttgtat tcgatcttaa catacgtagt acattgttat attccaatag tttgatttga
     1201 tttaattaaa gttcttactt taaatgtttt caggcttaaa ctccatgatt gcttaagaat
     1261 aagagtttac ttttccaaag tccacacaaa tgtaaaccac tcctctaaag agctacctgt
     1321 tttacttatg tgaatagcca taaacaaatg tttgtaatat acttatgtaa accaattctc
     1381 ctaaaagaat actctctgat ctatgctgat tatatgcatt cctactccct atccgtcctt
     1441 tttaaaagta gaactgttaa taatgtctta ctttttatct cggatgagca aatctaccct
     1501 tttttagaaa aagctgattt tcgccttaag ctctgagaat cctctgtacg tgtacaaatg
     1561 gacgatcggt ttcaaataaa cgattctcaa gaatcgaaaa aaacggtatg attgagtttt
     1621 ttttcctaca tttggcaaag aataaaactc tcgtaaatgg atattaaaag catactggac
     1681 ttcctgcacc tcttatcgca aaacaacaac agggaatggt tcaataaaca caaagctttt
     1741 tataccacaa gccgggagca tcatatacgg cttctcgaaa aacttatctt gctgctggct
     1801 gattttgatg ctgaattaag gcatttaagg cctcaggaat gcattttcag gatatatcgg
     1861 gacttacgct tcactcagga taaaacaccc tacaaaactt tttttggagc ttttatggcc
     1921 gcgggcggaa agaatagtga gcgggcggga tattacctgc acctgaaacc tgataactgt
     1981 ttcataaccg gaggcatatg gatgccggag gctcctcagc tgaaaatttt acgtcaatct
     2041 atcgtagaca atctggatga atttgaagat attgttcatt ctcaagactt tgtatctagt
     2101 tttggttctt tggacggacg aagacttcgg acggctccca aaggatttcc cgccgatttc
     2161 ccgggtatgg actatttgaa attcaaagat tataccgcca ctcacaagct tagcgccctg
     2221 gaaatggaac gctccgattt ttgtgaatac atcgccggtc tttttcaaca cctctaccct
     2281 ttgaaccgtt ttttgaattt tgcgttggac gaatacaaag agaacaaacc attttacaac
     2341 cacccaataa atagttaaac atgatacttg attcattaaa aaacagctct ctttacgaaa
     2401 gcatgcatcc tttattcaaa agagctttcg attacctgaa atcacgcgac ctggacttac
     2461 tggagccggg taagatagta ttggacggag ataaacttta tgtttcgatt tcggaatatg
     2521 aagggaaatc acccgaagca gcacgcctgg agacccacga aaaatacata gacattcagg
     2581 tcatcatcag cggcaccgaa accatgggct ggcgagccgc gaacgattgc aaacacgctc
     2641 ttgctcccta cgatacagtg aaagatatct gtttttacaa agataatccg gtaagtttcg
     2701 tcgaggttca tccgggagaa ttcacggtgt ttttccccgg tgacggacac gcaccctgca
     2761 taggcaaagg ccccatcaaa aaagccgtgg ttaaagtttt gctttaatga aatccaagac
     2821 attgaaaatc atcaagaacg atccttggtt ggaaccctac cgacaagcca tcgagggacg
     2881 ctataattac gctctgagca gggagaaaag cctgaccaag ggtggaagcc tgtccgaatt
     2941 tgctaatggg cacctttttt ttgggctgca taaacatcca gacggatgga cttttcggga
     3001 atgggctccc aacgccgatt ccatttattt gattggcgaa ttcaaccagt gggagcgtcg
     3061 acccgaatac gctctgcaga aaaaggaaca tggtgtatgg gaaatccatc tagccgccga
     3121 ttgtctgaag cacggagacc tgtacaaaat gtatgtctcc tggcctggcg gtagcggaga
     3181 gcggataccc gcctggacaa gacgggttgt tcaggatccg gttagcctta ttttcagcgc
     3241 tcaggtgtgg gagccggcaa atccttatcg ctttaaacat aaagtaaagc ctccctccca
     3301 acctctgttc atttacgaat gccacattgg catggccacc gaagaggaga gaataggcac
     3361 gtacgacgag tttagacgcc tggtcctacc gcgcatagtc gataacggct acaacgccat
     3421 tcagattatg gccatacagg aacatcccta ttacggttct ttcggctatc atgtttcaag
     3481 tttctttgca gcctcttccc gttttggcac acccgaagag ctcaaacggc ttattgatga
     3541 ggctcacggc tatggtctgg cagttataat ggatatagta cattctcatg ccgtcaaaaa
     3601 ttccgtcgaa ggcttaggtt gcctggacgg tgaccccaac ctgtactttc acagcggcga
     3661 cagaagagaa catccggctt gggattccct ttgtttcaat tacggccgcg atgaagtatt
     3721 acactttttg ctgtccaatt gtaaatattg gcttgatgaa ttccgttttg acggtttccg
     3781 cttcgacgga gttacttcta tgctctatcg cagccacggg ctgggagaat ccttcagttc
     3841 ctacgacgac tattataacc tgaatcaaga cggagacgcc atagcttacc tgactttggc
     3901 caataaactc attcacgaaa tcaaacccaa agccattacc attgccgaag aagtcagcgg
     3961 tatgcccggt ctggcggcct ccattaaaga tggcggaata ggttttgact atcgcatggc
     4021 catgggtatc cccgacttct ggatcaaact gataaaggaa tacagcgacg aagactggaa
     4081 gcccggagcc atctggtggg agttgactaa tcgcaggaaa gacgaaaaaa ccatcagtta
     4141 tgctgaaagc cacgatcagg ctttggtagg cgacaaaacc atcatattcc gtctgattga
     4201 tgccgatatg tactggcata tgagcaaaaa tgaccaaaat ctggtggtcg accggggcat
     4261 tgccctgcac aaaatcatac gtttattgac ggcgagcact ataaatggag gctatctgaa
     4321 ctttatgggc aatgaattcg gacatcccga atggatagat tttccgagag aaggcaacgg
     4381 atggtcctat aaatatgccc ggcgccaatg gagcctgctc gaaaaccggc aacttaaata
     4441 cgccgattta catgcttttg acaaagctat ggttatgttg ctgaaaaaaa tcaatcctaa
     4501 ggagccactc taccaacgtt ggcttcacga ccaggatcag attatggcct tcgagcgagc
     4561 ctctttccta tttgtcttca atttgcatcc ttccaggtcc ttcgtcgatt acggcatcct
     4621 ggtgcaaaaa ggcaactata aaatcgtctt aaacagcgat gccaaagaat tcggaggttt
     4681 tgggcttgtc aatgaacaaa ttgttcatac tacaacccag acggcaaaaa atcctgactt
     4741 cgagctggaa tggatacgcc tatacctacc ttccagaaca gctttggttc tgaaaaggca
     4801 ataagagcct tccttggcaa tagttgcaat tgaacgaatc aagaagtagg ttcaataaaa
     4861 caccagaagg caagctcgtg caaatcaatc ggtacggtaa accaatactg cccccttttg
     4921 agtccgaatt tcgaatgtgg aacccagggc ttcgttgaga cttccctgcc accaattagt
     4981 cagcctgcga tcaatcagag gatacttcaa tccttctgcg ttgactattg ttgacggatc
     5041 gattgcaaag agggagattt gctggccggg gtagctctgc agttgcttgt aatgcctgac
     5101 gacatctatg cgaccatagt ttgtttgcat acagatatcc tctattcgca aagagtaatc
     5161 cactaataat gagatgtttc ccaaagtatg atcttctctt ataccggtag ctcccataag
     5221 gataatttgc cggtaaccgg cacgttcaca atagttcacc gctttggtca aatcgttggt
     5281 ttcctgctca tcgatagtta tgatgcggtc ggcataaagc tctttcagtg agggactcag
     5341 gctgtccata tcgcccacaa taacagaggg ttctctaccg aaattcaaca atttttcggc
     5401 agctccatcg cagcaaacaa catgttctgc ctggcgtaat aagtgcagca acccgggttt
     5461 tcgggggaac tctccgttgg caaggatgat ggttggagac ataactaaat cttaaaaacg
     5521 aatttcaaca tataatgtct gggagccaat acataatagc cggtttcccg gtagggctct
     5581 tctccgctca gaaaagcgat ttcataaccc caggcattgc acaagggctg gttacccagt
     5641 aaattgttta tataaaagcc caattcaaag tcgctgttgc caactaaaca ccctgaatat
     5701 acggcagaaa agttaaactg ggtataggcc ggtaagcgaa attcctcgtg ctgaatatag
     5761 ctaaactgct g
//
LOCUS       DAOSGL010000121         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12465_length_5705_cov_4.239115, whole genome shotgun sequence.
ACCESSION   DAOSGL010000121 DAOSGL010000000
VERSION     DAOSGL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12465_length_5705_cov_4.239115"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKY68_04910"
     CDS             complement(<1..220)
                     /locus_tag="PKY68_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOH24412.1"
                     /translation="MFSSHTYIQRRNLLCRILDKGLVVLMGNNESPMNYADNTYPFRQ
                     DSCFLYYIGLDQPGLIALIDVDENQTLLF"
     gene            329..2398
                     /locus_tag="PKY68_04915"
     CDS             329..2398
                     /locus_tag="PKY68_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOH24413.1"
                     /translation="MINQELINPQSIVVVGGSNNVHKPGGRIVRNLIEGKYRGELYTV
                     NQKGEGDIQGVKNFKDARDIPEVELAILSIPAAACPAMVEILAKEKKVKAFIVISAGF
                     GEETKAGAVLEEQLLKIVEETETALIGPNCIGIMNMNYHGVFTQPVPEFHSEGVDFIS
                     SSGGTALFIIESALMKGLRFSSVWSVGNSKQNGVEDVLEYMDLNFDPLRDSKIKMLYV
                     EQIKDPDKMLYHASSLIKKGCKIAAIKAGSSDSGKRAASSHTAAIASSDSAVEALLRK
                     AGIVRCYSREELTTVAGIFTLKEAKGKNCAIITQAGGPAVMLADALSKGQINVPLLEG
                     ERVEELKTKLLPGAAVGNPIDLIGTGTPEHLATAIDYCEKHFDNINLMMVIFGSPGLV
                     KVYDAYEVLHKKMEECSKPIFPVLPSIVTAGPEVKSFVRKGHVNFSDEVTLGTALSRV
                     LNTPTPASSDVQLYGVDVPEVRKKIDHLNYSGFLSPQDVRSLLSLARIPLVNELVTGD
                     LDALKAFAQKTGYPVVLKVVGPVHKTDIGGVALNVRTSEHLQVEYERMMKIPEVTALL
                     IQPMLKGQELYIGANYEDRFGHVVFCGLGGIFVEILKDVSCGLAPLSYEETFSMIRSL
                     RSYPIIRGVRGQKGLGEQQYADIIVRLSTLLRFAKEIKEMDINPLIATDKGIFAVDAR
                     IRIEKQS"
     gene            complement(2419..4785)
                     /locus_tag="PKY68_04920"
     CDS             complement(2419..4785)
                     /locus_tag="PKY68_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOH24414.1"
                     /translation="MMTERIKQLRRESLEAINTINAERALLITEFYSKHLAFGDSVPV
                     QRAKSLQYILRNKSICINDNELIVGERGPAPKATPTYPEINVHSLKDLDILNSREKVW
                     FRVDKETRKAYEDIIIPFWKGRCNRDRIMESMSPEWLNAYRAGIFTEFMEQRAPGHTV
                     AGYKLFQKGMLDILEDIKQSRSNIDPLNDPAAYDKLEELKAMEIAAHAIMEYAERHAQ
                     KLEELATVEKNDQRRRELQQMAKICRRVPAHAPSCFHEVLQHYWFIHLGVITELNPWD
                     SFNPGRLDQHLYPFYKAETAAGSLTKEQAVEILQSFWIKFNNHPAPPKVGITAQESNT
                     YTDFSLINHGGLTTDGKDAVNEMSFILLDVIEEMRLLQPSSMVQLSEKNPDAFIERAL
                     KITKTGFGQPSIFNTEAIVQELLNQGKSLEDARNGGCSGCVEVGAFGTESYILTGYFN
                     LPKILEITLHDGFDPRTGKQIGLHTGKVDSFGSFDSFLVAYKSQVEHFVRIKLAGSNL
                     IESIFMRHIPTPFLSLFIDDCVAKGMDYVGGGTRYNTNYLQGVGLGTITDSLTAIKLH
                     VFDNKSIAFSEFVQALENNFKGAEELRYQLLYKTPKYGNDDDVADVHLAEVFDIYYRA
                     VSGHVSPRGAQYRINLLPTTCHVYFGSVMHASPDGRKSGLPVSEGISPVQGADKKGPT
                     SVLKSAAKIDHLHTGGTLLNQKFSPAFFESEDAIRKLGQLIRSYFKMNGHHIQFNVVD
                     AETLRDAQKNPERHQGLIVRVAGYSDYFNDLGVELQNEIIQRTEHESI"
     gene            complement(4806..5603)
                     /locus_tag="PKY68_04925"
     CDS             complement(4806..5603)
                     /locus_tag="PKY68_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOH24415.1"
                     /translation="MQGLVFDIKRFAVHDGPGIRTTLFLKGCPLSCAWCHNPEGTSPE
                     PQTVTKSISLDGQVFQEEEKVGLSMSVEQVMQELLKEKVFMDCSEGGVTFSGGEPLMQ
                     PAFLKSLLVACKKEGLHTAVDTSGYAKLSVIQDIAPYTDLFLYDLKLMDDQAHHHFTT
                     VSNKLILDNLIYLLEKRHPLRIRIPVIPEINFNQENTNECLRFLVAHPGNIQGVDLLP
                     YHNMANHKYLRFKKENSLSSLQSLPKSSLIAMKEQFEQAGYETKIGG"
BASE COUNT         1450 a         1257 c         1433 g         1565 t
ORIGIN      
        1 caaacaggag ggtttgattt tcgtcgacat caatgagcgc aattaatccg ggctggtcca
       61 gcccgatgta ataaagaaaa cagctgtcct gccgaaaagg ataagtatta tcggcataat
      121 tcatagggct ttcattattg cccattaata ctaccagacc tttgtccaat atgcgacaaa
      181 gcaggtttcg tcgttgaatg taagtatgag aagagaacat agcatatgtt tcggataaat
      241 atccgataaa aatttctgac agacaattta tacagctaaa aaaattatat ttgtcctttg
      301 tccggaaagt tcagtaaacc ttgaatctat gattaaccaa gaacttatca atcctcaaag
      361 catagttgtt gttgggggct caaacaatgt acacaagccg ggtggtcgta ttgtgcgtaa
      421 cctgatcgaa ggcaaatatc ggggagaact ctacaccgta aaccaaaaag gggaaggcga
      481 tattcaagga gtaaaaaact tcaaagacgc gagggatata ccggaggtgg agctggccat
      541 tctctctatt cccgccgctg cctgccctgc tatggttgaa atacttgcca aagaaaaaaa
      601 ggtcaaagcc tttattgtga tatcggccgg ctttggcgaa gaaaccaagg caggagctgt
      661 tttggaggaa caattgctca aaatcgtgga ggaaactgaa acagcactga ttggcccgaa
      721 ttgcataggt attatgaata tgaattatca tggcgtgttt acccaaccgg ttcctgaatt
      781 tcatagcgag ggtgtagatt ttatttccag ttcgggaggt acggcccttt ttatcattga
      841 gtctgcattg atgaaaggtt tgcgtttttc ttcggtttgg tcggtgggaa acagtaagca
      901 aaacggagtg gaagacgtgt tggaatacat ggatctgaat tttgatccct tgcgcgattc
      961 taaaattaaa atgctgtatg tggagcaaat caaggatccc gacaaaatgt tgtatcatgc
     1021 ttcctcactt atcaagaaag gttgcaaaat agccgctatt aaggccggca gtagcgattc
     1081 cggaaaaaga gcggcttctt cacatacggc agccatcgcc agcagcgatt cggcggtgga
     1141 ggccctgttg cgtaaagccg gtattgttcg ctgctatagc cgcgaagaat tgacgacagt
     1201 agccggtatt tttaccctca aagaagcaaa aggaaaaaat tgcgccatca ttactcaggc
     1261 cggaggtccg gccgtgatgt tggccgatgc cttgtccaag gggcagatca atgtaccctt
     1321 gctagagggg gaaagggtag aggaattgaa aaccaaatta ctgcccggag cggccgtagg
     1381 caaccccatt gaccttattg gaacgggcac tcccgagcat ctggcaacgg ctatcgatta
     1441 ttgtgaaaag cattttgaca acatcaacct gatgatggtt attttcggca gtcccggtct
     1501 ggtaaaggtt tacgacgcct acgaagtgtt gcacaaaaaa atggaagaat gttccaaacc
     1561 gatttttccc gtcctgcctt cgattgtaac ggccggcccc gaagttaaaa gctttgtcag
     1621 gaagggacac gtcaattttt cggacgaagt gaccctgggg actgctctct cgagggtatt
     1681 gaatactccg acacctgcaa gcagtgacgt ccagctctat ggtgtggatg tgcccgaagt
     1741 ccgtaaaaaa attgatcacc tcaattacag cggtttttta tcgccgcaag atgtgcgttc
     1801 cctgctgagt ctggccagaa ttcctttggt caacgaattg gttaccggcg atttagatgc
     1861 tctgaaggct tttgctcaaa aaacaggcta tccggtggtt ttaaaagtgg tgggtcctgt
     1921 tcataaaacc gacattggcg gtgtggccct gaatgtgcgc accagcgagc atttgcaagt
     1981 ggaatacgag cgcatgatga aaattcccga agtgactgct ctgctgattc agcccatgtt
     2041 aaaaggacag gagctctata tcggcgccaa ttacgaggat cgtttcgggc atgtggtttt
     2101 ttgcggtctg ggcggcattt ttgtagaaat tcttaaagac gtttcctgtg ggctggctcc
     2161 tctatcgtac gaggaaactt tttcgatgat acgttctcta cgcagttatc ccattatcag
     2221 aggagtccgg gggcaaaaag gcctgggcga acagcagtac gccgatatta tcgttcgctt
     2281 gtccacactt ttgcgttttg ccaaagaaat caaagagatg gacataaatc ccctgatagc
     2341 cacagacaaa ggaatctttg cagtggacgc cagaatcagg atagaaaaac agtcttaatg
     2401 ctaaaattgg tttccggctt agattgattc gtgttcggtc cgctggataa tttcgttttg
     2461 gagttcaaca cccaggtcgt tgaagtaatc gctgtacccg gccaccctta ctatcaggcc
     2521 ctgatgccgt tcgggatttt tttgagcgtc tctcagcgtt tctgcatcta ccacgttgaa
     2581 ttgaatatgg tggccattca ttttgaaata ggaccggatc aattgaccca gtttgcgtat
     2641 ggcatcttcg ctttcgaaga aagcggggct gaatttctgg ttaagcaggg tgccaccggt
     2701 atgcaagtga tcgatttttg ccgccgattt cagcacggaa gtagggcctt ttttgtcggc
     2761 tccctgcaca ggggaaatgc cttcggaaac cggcaggccc gatttacgtc cgtcggggct
     2821 ggcatgcata acgctgccga aatacacgtg acaggtagta ggtaaaagat tgatgcgata
     2881 ctgtgcccct ctgggactga cgtgtccgct gacggcacga taataaatat cgaagacttc
     2941 ggctaggtga acatcggcca catcgtcgtc gttaccgtat ttaggggttt tatacagcaa
     3001 ctgatagcga agttcctctg cccctttgaa gttgttttca agggcttgta caaactcgct
     3061 gaaagctatg cttttgttgt cgaaaacatg cagcttgatg gcggtcagag aatcggtgat
     3121 ggtccccagg cctacaccct gaagataatt ggtattgtag cgggttcctc ctcccacgta
     3181 atccatgcct tttgcaacac aatcgtctat gaataacgaa aggaatgggg tgggaatatg
     3241 cctcataaaa atgctttcga tcagattgct tccggccagc ttgatcctga caaagtgttc
     3301 aacctgagat ttataagcta ccagaaaaga gtcaaatgaa ccaaaagagt ccactttgcc
     3361 ggtatgcagg ccaatctgtt taccggtgcg gggatcgaag ccgtcgtgca gggttatttc
     3421 caggattttt ggcaggttga aatagccggt caggatatag ctttcggtgc cgaaagcccc
     3481 cacttcgacg cagccgctac aacctccgtt acgggcgtct tccaggcttt ttccctgatt
     3541 taagagctct tgtactatgg cttcggtatt gaaaatggaa ggctgcccaa aaccggtttt
     3601 ggtgattttt aaggcacgtt caataaaagc atcgggattt ttttcgctca gctgcaccat
     3661 agaactgggc tgcagcaacc tcatttcttc gataacatcg agcaggataa aactcatttc
     3721 gttcaccgca tctttaccgt cggtggtaag gcctccgtga ttgatgagcg agaaatcggt
     3781 ataggtgtta ctctcctgag cggtaatgcc cactttggga ggagccggat ggttgttgaa
     3841 cttgatccag aaagactgca ggatctcgac ggcttgttct ttagtaaggc ttccggctgc
     3901 tgtttctgct ttgtaaaaag gataaaggtg ctggtccagt cgcccgggat tgaaagaatc
     3961 ccagggattg agctcggtaa tgactcctag atggatgaac cagtaatgtt gcagcacttc
     4021 gtgaaagcac gagggagcat gggccggaac acgacggcag atttttgcca tttgttgcag
     4081 ttcccggcgg cgctgatcgt tcttttcgac ggttgccagc tcttccagtt tttgggcatg
     4141 gcgctcggcg tactccataa tggcatgggc cgcaatttcc atggccttga gttcttccag
     4201 cttgtcgtag gctgccggat cgttcagggg atcgatgtta gaacggcttt gttttatgtc
     4261 ttcgaggatg tcgagcatgc ctttttgaaa caacttatag cctgccaccg tgtgtccagg
     4321 agcccgttgt tccataaatt ccgtaaatat gccggcccga taagcattta gccattcagg
     4381 gctcatgctc tccataatgc ggtcgcggtt acaacgtcct ttccagaatg gaatgataat
     4441 gtcttcgtaa gctttccggg tttccttatc cacacggaac cagacctttt ctctggaatt
     4501 aaggatatcg agatccttca gcgaatgaac attgatttcg gggtaggtgg gagtagcttt
     4561 gggagccggc ccgcgttcgc ctacaatcag ttcgttgtcg ttgatgcaga tgcttttgtt
     4621 tctcaaaata tattgcagag acttagcccg ctgaaccggt actgagtcgc caaatgcgag
     4681 gtgcttgctg tagaattcgg tgatcagcaa ggctctttcg gcattgatgg tgtttatagc
     4741 ttccaggctt tcccggcgaa gttgtttaat acgttcggtc atcataaatg ttaaatggat
     4801 aaatgtcaac ctccgatttt tgtttcgtat ccggcttgct caaattgctc cttcatcgct
     4861 attaaactcg attttggcag actttgcaag gagctcagcg aattctcttt tttaaatcgc
     4921 aggtatttgt ggttggccat attatggtaa ggtagcagat ccactccctg aatattgccg
     4981 ggatgggcta ctaaaaaacg caagcattcg ttggtgtttt cctgattgaa attaatttcg
     5041 ggaatcacag gaatgcgtat tcttagagga tgccgcttct cgagcaaata aatcagattg
     5101 tcgagaataa gcttgttgga gacagtcgtg aaatgatgat gggcttggtc gtccattagt
     5161 ttcagatcgt ataaaaaaag atcggtataa ggcgcaatat cttgaataac agatagcttt
     5221 gcgtatcccg aagtgtctac ggccgtgtgc aatccttctt ttttgcaagc gacaagcaaa
     5281 ctttttaaaa aggcaggctg catcaggggt tcgcctccgg aaaaagttac acccccttct
     5341 gaacagtcca taaagacctt ttctttgagt aattcctgca taacctgttc gacagacata
     5401 cttaacccca ccttctcttc ttcttgaaac acttgcccgt ctaggctgat gcttttagtg
     5461 acggtttgag gttcagggct ggtgccctcc ggattgtgac accaggcgca actaaggggg
     5521 caacctttca aaaacaaggt tgttctgatg cccggtccgt cgtggacggc aaaacgcttg
     5581 atgtcgaaaa ccagcccttg catattagtt tttcaaacgt tcgtactcac aggaagccat
     5641 ggtgaacgaa cctatggcct tgccttcgtt ctgggtgatg gtaacatcgc ttccgcaaag
     5701 gtaat
//
LOCUS       DAOSGL010000122         5691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12524_length_5691_cov_3.863201, whole genome shotgun sequence.
ACCESSION   DAOSGL010000122 DAOSGL010000000
VERSION     DAOSGL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12524_length_5691_cov_3.863201"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKY68_04930"
     CDS             complement(<1..321)
                     /locus_tag="PKY68_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24416.1"
                     /translation="MKLKRFLLIYFLVVLAGVFCFSCIDQSYDLEKLSGKMELFGNSI
                     SVPIGTTTIYLDSVVGNLTQDSGSLTVKDGVYVFEFAGSMDMSGLTSALGDFSLAAVD
                     PIMTT"
     gene            complement(562..1722)
                     /gene="lysA"
                     /locus_tag="PKY68_04935"
     CDS             complement(562..1722)
                     /gene="lysA"
                     /locus_tag="PKY68_04935"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOH24417.1"
                     /translation="MKGCFPLSSFNSLRTPFYYYDLDVLRSTLDLLRKEADRNEMEVH
                     YAVKANANPRILDIIRAYGFGADCVSGGELQAAFEAGFPASGLVFAGVGKADWEIDLG
                     LEGNIFCFNVESVPELEVIDQRAGAMGKKAGVALRINPNVQADTHHYITTGVNDSKFG
                     ISLQSLDEVLDICLKLQHIELIGLHFHIGSQITEMDNFAGLCARVNEIQNLLDRRRLF
                     VQHINLGGGLGVDYHHPDQVPVPDFADYMQTLRRMLVLRPGQSLHIEPGRSVVAQCGS
                     LISKVLYIKKGLQNEFVILDAGMTELIRPALYQAYHKIENISSEELEQKYDVVGPICE
                     SSDCFGKQVVLNKTRRGDLIAIRSTGAYGETMASNYNLRPKAPAVFSDDLLS"
     gene            complement(1731..3044)
                     /locus_tag="PKY68_04940"
     CDS             complement(1731..3044)
                     /locus_tag="PKY68_04940"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOH24418.1"
                     /translation="MKVMKFGGTSVGSAQRMKEVAGIVAKEGKNIIVLSAMSGTTNTL
                     LEISDYLHKRNPDGANEIINMLELKYLREIEDLFESKEYKIQTLEVIKSHFDHIRSFT
                     KKVFTLFEEKVIVAQGELLSSVMFTNYLKEQGLNAALLPALEFMRINKNAEPDQNYLR
                     EHLNALLQQHQDADVYITQGFICLNAYGEIDNLQRGGSDYTASLIGAAVKAKEIQIWT
                     DIDGMHNNDPRIVDNTSPVSFLNFDEAAELAYFGAKILHPTCILPAKLEKIPVRLLNT
                     MKPNAKGTTISNKSEKHKIKAVAAKDNITAIRIKSGRMLLAYGFLRKVFEIFESYRTP
                     VDMVCTSEVGISMTIDNNKHLKEILDELKKIGTVSVDRDMVIICVVGDLTWDNIGFEA
                     KVVEALKDIPVRMISYGGSNYNISLLVKAEDKKRSLIALSEQLFK"
     gene            complement(3049..3741)
                     /locus_tag="PKY68_04945"
     CDS             complement(3049..3741)
                     /locus_tag="PKY68_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH24419.1"
                     /translation="MEDTILIHFDKVDICLEDFIVLKEVSFDIRPGEFIYLIGRVGSG
                     KSSLLKSLYAEIPVTEGKARIYDYNLHKIKRKQVLALRRGLGIVFQDFKLMTDRTVWN
                     NLNFVLRATGWKDKNEIEERIDKVLTQVGMQNKGYKMPHELSGGEQQRIVIARALLNN
                     PRIILADEPTGNLDAETGDAIVQLLHDIKDKGTTVLMTTHNLGFLDKFPGRVFKCDHF
                     RLSEETNTIKME"
     gene            complement(3743..4339)
                     /gene="hisIE"
                     /locus_tag="PKY68_04950"
     CDS             complement(3743..4339)
                     /gene="hisIE"
                     /locus_tag="PKY68_04950"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOH24420.1"
                     /translation="MKPDFAKMGGLVPAIVQDHVTGKVLMLGFMNQEALDKTLETGKV
                     TFFSRSKGRLWTKGEESGHFLHLVSVKEDCDKDTLLIQANPEGAVCHLGWDTCFNEKN
                     SSGDLDFLLYLQDFIDRRYREMPEGSYTTSLFMEGVKRMAQKLGEEAVETLIEAIDGN
                     DERFLYEASDMLYHLMVLLTSKGYRLEDLARELKKRHK"
     gene            complement(4342..5100)
                     /gene="hisF"
                     /locus_tag="PKY68_04955"
     CDS             complement(4342..5100)
                     /gene="hisF"
                     /locus_tag="PKY68_04955"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOH24421.1"
                     /translation="MLAKRIIPCLDIKDGQTVKGVHFVDFRRAGDPVELGEAYSKQGA
                     DELVYLDITASYEGRKTFTDLVSKVAARISIPFTVGGGIHELADIDRLLEAGADKVSI
                     NTAALLKPQLIDDIARNFGSQICVVAIDAKLEEDGKWRCYINGGHRPVERELLDWALE
                     AQDRGAGEILFTSMSHDGVKNGYPNEALAMLADRLSIPLIASGGAGTMEHFKEAFVLG
                     KADAALAAGVFHFGEIKIPELKAYLKKEGITVRE"
     gene            complement(5094..>5691)
                     /locus_tag="PKY68_04960"
     CDS             complement(5094..>5691)
                     /locus_tag="PKY68_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HOH24422.1"
                     /translation="AGIRRLHLVDLDGAKAQHIVHHRILEQITSHTSLIVDFGGGLKT
                     DEDLRIAFESGAQMVTGGSIAVKSPEAFENWINTYGPERIILGADVSEGKIAVSGWTE
                     RSELDLWSFLENYVQKGINKVICTDISRDGMLRGPALELYTQILAKYPELHLIASGGV
                     SSLEDLDDLKAAGLPAAILGKAIYEGRIQLKDLSKYIC"
BASE COUNT         1449 a         1443 c         1228 g         1571 t
ORIGIN      
        1 agtagtcata atcggatcta ctgctgccaa cgaaaagtct cccagagccg aagtcagccc
       61 gctcatatcc atgctgcctg caaactcaaa aacataaact ccgtctttta cagtcaacga
      121 ccctgaatcc tgggtcaagt ttccaactac actatcgaga tagatggtag tagtgccgat
      181 tggaacggat atggaattgc caaaaagctc cattttgccc gaaagctttt ccaggtcgta
      241 cgactgatct atacaggaaa aacagaaaac tcccgctaat acgacaagga aataaattaa
      301 tagaaagcgt tttagtttca tagtcttatt tttttaatgg aaaatacaac ctgtcacgag
      361 ggtacaatat tactatattt tttggttcct cctactttca attgaaaaat actttagccc
      421 ttattgccca tacagagggc taaattagta aagcaacgac agaaaacaaa aaaatctcta
      481 agccctttcc ggcttcaaca tcataaatcg tcggaaaaaa acagctgaaa ggttttgccg
      541 gttgtcggac cgcagaaggt ttcaagatag taaatcgtcg gaaaaaacag caggggcttt
      601 aggtcttaag ttgtagtttg aagccatggt ttctccatag gctccagtag aacgtatggc
      661 tatcaggtct ccccgccggg ttttgttgag tactacctgt tttccaaaac aatcggacga
      721 ttcacaaatg gggccgacca catcgtattt ttgctcaagt tcttccgaac tgatattctc
      781 aatcttgtgg taggcctgat acagagccgg ccgtataagt tcggtcatgc cggcatccaa
      841 aataacaaat tcgttttgca agcctttctt tatgtacaaa actttggaaa tcaaactgcc
      901 gcattgagcc accaccgaac gtcccggctc aatatgcaaa ctttgtccgg gccttaatac
      961 caacatcctg cgtaaggtct gcatataatc ggcgaagtcg ggaacaggca cttgatccgg
     1021 atgatgataa tccacgccca gtcctcctcc cagattgatg tgttgaacaa aaagccggcg
     1081 ccggtccaga aggttctgaa tttcgttcac cctcgcacaa agacctgcga aattgtccat
     1141 ctcggtaatt tgcgaaccga tatgaaaatg caagcctatc agttcaatat gctgcagttt
     1201 caaacaaata tcgagtacct cgtcgagact ttgcaacgaa ataccgaatt tactgtcgtt
     1261 cacgccggtg gtgatgtaat gatgcgtatc ggcctgtacg ttggggttga ttcttaaagc
     1321 cactccggcc tttttgccca tggccccggc ccgctgatct attacttcca gttcgggaac
     1381 cgattctacg ttgaaacaaa aaatattccc ctcaagtccc aggtctattt cccaatcggc
     1441 tttacccact cccgcaaaca ccagcccgga agccgggaaa ccggcctcaa aagcagcctg
     1501 caattcacct ccgctcacac aatcggcgcc aaagccgtaa gcgcggataa tatcgagaat
     1561 acgtggattg gcattggctt ttacggcata atgcacctcc atttcgtttc tgtccgcttc
     1621 tttacgaagc aaatccaggg tggaacgcag tacatccagg tcgtaataat aaaaaggagt
     1681 tcgaaggctg ttgaaactgc ttaaggggaa acatcctttc ataacatatt ttatttaaac
     1741 agttgttcgc tcagggcaat caaactgcgt tttttgtctt cggccttcac cagcagggaa
     1801 atattgtaat tgcttccccc gtaagaaatc atccggaccg gaatatcctt gagggcctcc
     1861 accactttgg cttcgaaacc gatgttgtcc caggtgaggt cgcccaccac acaaataatg
     1921 accatgtcgc ggtcgacgct gacggttcct atttttttca actcatccaa aatctctttc
     1981 aggtgcttat tgttgtcgat ggtcatcgaa atacccacct ccgaggtgca aaccatatcc
     2041 acgggagtac ggtaactttc gaaaatttcg aacaccttgc gcaagaaacc gtaagccaaa
     2101 agcattcggc ccgactttat acgtatggcg gtaatattgt ctttggcggc taccgctttg
     2161 attttatgct tttcgctctt attcgaaatg gttgttccct tagcattggg tttcatggtg
     2221 ttaagcaagc gtacgggaat cttttcgagt ttggcgggaa gtatgcaggt ggggtgtaat
     2281 attttggctc cgaaataggc caactcggcc gcttcgtcga aattcagaaa cgacaccggc
     2341 gaagtattgt ccacgattct ggggtcgttg ttgtgcatac cgtcgatatc ggtccagatc
     2401 tgaatttctt tggcttttac ggctgctcca atcaaagaag cggtataatc gctgccgccc
     2461 ctttgcaaat tatctatttc gccatacgca ttcaaacata taaaaccctg ggttatatat
     2521 acatcggcat cctggtgttg ttgcaataaa gcgttcaaat gttcacgcaa gtaattttgg
     2581 tcgggttcgg cgtttttatt gattctcata aattcgagag ccggcaaaag agcggcattc
     2641 aagccttgct cttttaaata attggtaaac atcacgctgg ataaaagttc cccctgcgcc
     2701 actatcactt tttcttcgaa cagggtaaat acttttttgg taaaactgcg aatatgatcg
     2761 aaatgcgatt taatgacttc gagggtttga attttgtatt ctttgctttc gaataggtct
     2821 tctatttcgc gtaaatactt taattccaac atattgataa tctcgttggc tccatcagga
     2881 ttacgtttat gcaaataatc cgaaatttcg agcagggtat tggtggtgcc cgacatggcc
     2941 gacaacacta tgatgttttt gccttccttg gcaacaattc cggctacctc tttcattcgc
     3001 tgcgcagaac ccacagaggt tccgccaaat ttcatcactt tcattttatt attccatttt
     3061 tattgtatta gtttcttcgc tcaagcggaa atgatcgcat ttgaataccc tgccgggaaa
     3121 tttatccaga aaacccaggt tatgagtggt catcaaaacg gtagtgcctt tatccttgat
     3181 atcgtggagc aattgcacaa tggcatcgcc tgtttccgcg tccaggttgc cggtgggttc
     3241 atcggccagt atgatacggg gattattgag cagggccctt gcaattacta ttcgctgctg
     3301 ttcgccgccc gacagttcgt gaggcatctt gtagcctttg ttttgcatgc ccacctgggt
     3361 aagcacttta tcaatgcgtt cttctatctc gttcttgtct ttccagccgg ttgctcttaa
     3421 aacaaaattc agattgttcc agaccgtccg gtctgtcatt aatttaaaat cctggaaaac
     3481 aattcccaaa ccacggcgta aagccaacac ctgcttgcgt ttgattttgt gcagattgta
     3541 gtcgtaaatg cgggctttac cttcggttac gggaatctcg gcatagaggc ttttgagcag
     3601 gctgctcttt cccgatccca ccctgccgat gaggtaaata aactcaccgg gacggatatc
     3661 aaaactcacc tccttgagta cgataaaatc ttccaggcag atatcgactt tgtcgaaatg
     3721 gatcagaata gtgtcttcca ttttatttat gtcttttttt caattcacgg gccaggtctt
     3781 ccaatcgata gcctttggag gtcaacaaca ccatcaggtg gtagagcatg tcggaggctt
     3841 cgtacaaaaa acgctcgtcg ttgccgtcta tcgcttcgat cagggtttcg accgcctcct
     3901 ctcccagctt ttgagccatg cgtttcactc cttccataaa cagcgaagta gtataagaac
     3961 cctcgggcat ttcgcggtaa cgacgatcga taaaatcttg caaataaagc aaaaaatcca
     4021 gatccccgga actgtttttt tcattaaaac aggtatccca acccagatga cataccgctc
     4081 cttcgggatt agcctgaatc aacagcgtat ccttgtcgca atcctctttt acagagacca
     4141 gatgcagaaa atggccgctt tcttcgccct tggtccaaag tcggcctttg ctgcgactga
     4201 aaaaagttac cttgccggtc tcgagggttt tatcgagggc ctcctgattc ataaagccga
     4261 gcatcaacac cttaccggtg acatgatcct gtactatggc gggaaccaag ccgcccattt
     4321 tagcaaaatc gggtttcatc gttattctct tactgttatg ccttcttttt ttaaataagc
     4381 tttcagctcg ggaattttta tttctccgaa atgaaagacg ccggctgcca atgcagcatc
     4441 ggctttaccc aaaacaaatg cctccttgaa atgttccatc gttccggctc ctccggaagc
     4501 tatcagaggg atactcaggc gatcggccag catagccaaa gcctcgttgg ggtaaccgtt
     4561 ttttactccg tcgtggctca tactggtaaa aagaatttcg ccggccccgc gatcctgagc
     4621 ttccaaagcc cagtcgagca attccctttc gaccggccgg tgtccgccgt tgatgtaaca
     4681 acgccatttg ccgtcctctt ccagtttggc gtcgatggcc accacacaga tctgcgaacc
     4741 gaaattgcgg gcaatgtcgt cgatgagttg aggttttagc aaagcggccg tattgatgga
     4801 gaccttatcg gctccggcct ccagcaaacg gtcgatgtcg gccagctcgt gtatgccgcc
     4861 ccccacggta aagggaatac tgattcgggc tgccaccttg ctcaccagat cggtaaaagt
     4921 cttgcggccc tcgtaagagg ccgtaatatc gaggtatacc aattcgtcgg caccttgctt
     4981 gctgtaggcc tctcccagtt ctacgggatc gccggctcgc cgaaaatcga caaaatgaac
     5041 gcccttgacg gtttggccgt ctttgatatc caaacaggga ataatgcgct tagctaacat
     5101 atatacttac ttaagtcctt caattgaata cgcccctcgt aaatggcttt gcccaaaatc
     5161 gccgccggca ggcccgcggc tttcaggtcg tcaagatcct caagactact cactcctccg
     5221 ctggcaatca ggtgaagttc cggatacttg gccaatatct gagtataaag ttccagagcc
     5281 ggtccccgga gcatgccgtc gcggctgatg tcggtacata tcactttgtt gatgcctttt
     5341 tgaacgtaat tctccagaaa gctccacaaa tccagctcgg agcgttcggt ccaaccggat
     5401 acggcaattt taccttcgct gacatcagct cccagtataa tgcgctccgg accgtaggtg
     5461 ttgatccagt tttcgaaggc ctccggcgac ttaaccgcaa tgcttcctcc ggtcaccatt
     5521 tgagctccac tttcgaaagc aatgcgcaaa tcctcatcgg ttttgagccc tccgccaaaa
     5581 tcgacaatca gggaagtatg gctggtaatc tgctccaaaa tccgatgatg cacaatgtgt
     5641 tgagcttttg ctccatccag atcgaccagg tgcaaacggc gaataccggc t
//
LOCUS       DAOSGL010000123         5684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12548_length_5684_cov_2.988453, whole genome shotgun sequence.
ACCESSION   DAOSGL010000123 DAOSGL010000000
VERSION     DAOSGL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12548_length_5684_cov_2.988453"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1843
                     /locus_tag="PKY68_04965"
     CDS             326..1843
                     /locus_tag="PKY68_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOH24423.1"
                     /translation="MIIGKRLNLEEFRAIVEGKAKIDIDDEVKKTVVECFDFLEDFSK
                     DKVIYGINTGFGPMSQWRIPDAHLIELQYNIIRSHSTGAGEPLPAIYVRASMLSRLMT
                     FLQAHSGVNFQTCRLLVEFINREIYPLIPQHGSVGASGDLVQLAHVALALIGEGEVFY
                     KGERRDTAEVFAELGLEPLKMYIREGLAITNGTAVMTGIGLVNLLDAKRLMEWSLKAS
                     VLMNEIASSYDDFMSGVLNGLKHHSGQKIVASKMRQIASGSRMLRSRENELYRGKDQQ
                     SFSNKVQPYYSLRCVPQVLGPVWDTMENAEKVLIDELNSVDDNPIVDPHGKTVVHGGN
                     FHGDYVSFEMDKLKMAVTKMTMLAERQMNYLFHDRINGILPPFVNLGVLGLNYGLQAS
                     QFTATSTTAECQTLSNPMYVHSIPNNNDNQDIVSMGTNSALICRQVIENSYQVMAIHM
                     IALAQAVDCLGAGDKLAPSTRKLYEELRLLVPAFVEDTPKYKEIERIENRLRGGL"
     gene            1871..2608
                     /gene="fabG"
                     /locus_tag="PKY68_04970"
     CDS             1871..2608
                     /gene="fabG"
                     /locus_tag="PKY68_04970"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOH24424.1"
                     /translation="MNPTKYALVTGASRGIGRAIALKLSTEGYKIIINYASNTAAAEK
                     TLSDIKALGGSAELLQFDVSDSKATLEALQTWKSKHPEEYISVLVNNAGIRKDNLMMW
                     MPQEDWRDVLSVSLDGFFHVTQALLKDMLVKKWGRIVNIVSLSGIKGMPGQANYSAAK
                     GGLVAATKALAQEVAKKRVTVNAVAPGFIKTDMTADLNEAELKSLIPAGRFGEPSEVA
                     ELVAFLVSESAAYITGEVISINGGIYT"
     gene            2615..3832
                     /locus_tag="PKY68_04975"
     CDS             2615..3832
                     /locus_tag="PKY68_04975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOH24425.1"
                     /translation="MAQRVVITGMGIYSCIGKNKEEVLKSLFVGKSGIGIVPERRELG
                     YRSALSGILEKPNLKELLDRRQRLCLSEHGHYAYVATLEALHQASISPDYLKLNEVGI
                     LYGNDSSAEAVINGVDIMREKKNTVLVGSGNIFQSMNSTITMNLSTIFNLRGINFTVS
                     GACASGSHAIGMGYLLIRQGLQDCVICGGAEEVNKYSVGNFDALSAFSIREDEPAKAS
                     RPFDKNRDGLVPSGGAATLILESYDSARKRGAAILGEVIGFGFSSNGDHISVPNIDGP
                     VRSLNMAIRDAGVKPGDIKYINAHATSTPIGDINEAKAILEVFGEHKPYVTSTKSQTG
                     HEMWMAGASEVIYSILMMQNNFIGANLNFEEPDEVSINLNIPAKRVNTAFDMFLSNSF
                     GFGGTNSSLIIKK"
     gene            3845..4090
                     /locus_tag="PKY68_04980"
     CDS             3845..4090
                     /locus_tag="PKY68_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOH24426.1"
                     /translation="MTIEEIVEKATTILAEEFEVEESEITPEASLKETLGLDSLDLVD
                     VVVLVEQHFGVTLKGQDFVGIKTFNDFYELLNRKING"
     gene            4083..4985
                     /locus_tag="PKY68_04985"
     CDS             4083..4985
                     /locus_tag="PKY68_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOH24427.1"
                     /translation="MAKEPEKQWEGKSRGGRWGYTFFISTIRLLGIRCAYFFLALVVL
                     YFIPFAPKATKSVWLYHRRRRKLGVLRSLRELYCHYYVFGQTLIDKVAMNAGMTNRYN
                     YVFDNYERFLDLLNSGQGVVMIGAHVGCWEAGAGFFGEYGKKINIAMFDVEHQQIKEL
                     LESNSSSKNNYKIIAINQDAISAVLQMKIALNNGEYLCFNGDRYVDKNNARAVEFMNA
                     RANFPIGPFRIAAKCGAPVVFYYAMREPARTYRFIFEEAGIEGKNSEQNITDQYVKSL
                     ERILKSYPRQWFNFYDFWNESIIV"
     gene            4989..>5684
                     /locus_tag="PKY68_04990"
     CDS             4989..>5684
                     /locus_tag="PKY68_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOH24428.1"
                     /translation="MKLSKPLEDIYGKEQQTALGAQRRAQEIAFAPMVFQVSRLMVKF
                     GILDYLAKSRNGATQQAIVEHTGLSKYAIQVLLEASLSIGTVLIKQDRYEISKAGRFL
                     LNDPMARVNMNFNQDVNYLGLFHLEEALREGKPAGLKVFGNWATIYEALSELPEHVQK
                     SWFAFDHFYSDESFAAALEVVFGSNRKINRLLDVGGNTGRWALQCVDYDKDVEVTIMD
                     LPQQLEMMRKNIAG"
BASE COUNT         1640 a         1122 c         1416 g         1506 t
ORIGIN      
        1 tccatatcca agtcttcaac agttacgtcc caaaacaaat tgggtgaaaa ttccgtaata
       61 ggattcttct tgtcttcttc tttttccatt gggaacctgt gattattttt ctttttcaaa
      121 agtaagaaga aaaaattgaa cagaaaagcc atttagctga tattcataat atatcaaaat
      181 cagtcctaaa aacattttga gataaaaaca aaaaatgctt acttcacagc ctaattccag
      241 gctgccctat gtagagggca gcttacatta aggacttttg taaaccgttt gtattgtctt
      301 tttaaaaaaa tgcgcaagaa ataatatgat tattggtaag aggttaaatc tggaggaatt
      361 tcgggccatt gtagagggaa aggctaagat agatatcgat gacgaagtaa aaaaaactgt
      421 agtagagtgt tttgattttt tggaggattt ttcgaaggac aaggttattt acggtatcaa
      481 taccgggttt ggtccgatgt ctcaatggcg tattcctgat gcgcacctta tagagttgca
      541 atacaacatc attcgcagtc attccaccgg tgcaggcgaa cctctgccgg cgatttatgt
      601 gcgcgcctcc atgctttccc gtctgatgac tttcctgcag gcacattcgg gagtgaattt
      661 tcaaacctgc cggttacttg tagagtttat caacagagag atatacccct tgattccgca
      721 gcatggaagt gtgggagcaa gcggagattt ggtacagttg gctcatgtcg ctttggctct
      781 gatcggagag ggcgaggtgt tctataaagg cgagcgtaga gatactgccg aagtgtttgc
      841 agagttgggc ctggagccct tgaaaatgta tattcgtgag ggcctggcaa taaccaacgg
      901 aacggcggtt atgacgggaa taggcctggt caatctcctt gacgcgaagc gactgatgga
      961 gtggtcgctg aaagcctcgg tattgatgaa cgaaatagcc tcttcatacg acgattttat
     1021 gtccggtgta ttaaacggac tcaaacacca ttcagggcaa aaaatagttg cctcaaagat
     1081 gcggcagata gcttcaggca gccggatgtt gcgtagcaga gaaaatgaat tgtatcgggg
     1141 aaaagaccaa cagtcgtttt ccaataaagt tcaaccatac tattctttgc gttgtgtacc
     1201 acaggtttta ggaccggtat gggacactat ggagaatgcc gaaaaggttt tgatagatga
     1261 gttgaactca gtagacgaca accctattgt agacccacat ggcaaaacgg ttgttcatgg
     1321 cggcaatttc cacggtgatt atgtctcttt tgagatggac aagctgaaaa tggccgttac
     1381 caagatgact atgcttgcag aacgacagat gaattatctg tttcacgacc gtatcaatgg
     1441 catactaccg ccctttgtga atcttggtgt gctcggatta aactacggtt tgcaagcttc
     1501 tcagtttaca gccacctcta caacggctga gtgtcagacg ctttcaaatc cgatgtatgt
     1561 tcactctata ccaaacaata acgacaatca ggatatagta tcgatgggaa caaattccgc
     1621 cttgatatgc agacaggtga tagaaaacag ctatcaggtg atggctatcc acatgattgc
     1681 tttggctcag gcggtcgatt gtctgggagc gggcgataag ctggctccgt ccactcgtaa
     1741 actttacgag gagctgcgtt tacttgtgcc ggcttttgta gaagatactc caaaatacaa
     1801 agagatagag aggatagaaa ataggctaag aggagggctg taaaccgaca acagattgat
     1861 aagaatggaa atgaacccaa ctaaatatgc gttagtaaca ggtgccagtc gcggcatcgg
     1921 acgggcaatt gctctaaagc tctccaccga aggttataaa ataattataa attatgcatc
     1981 caatacggca gcagccgaaa aaaccttaag cgacattaag gctctcggcg gtagcgctga
     2041 actcttacag ttcgatgtta gcgatagcaa ggctacgctc gaggcacttc agacgtggaa
     2101 gtcgaaacac cctgaggagt atatttccgt gttggtcaat aatgccggta tccgtaagga
     2161 taaccttatg atgtggatgc cacaggagga ttggcgcgat gtgctttctg tctcgctcga
     2221 cggatttttc catgttaccc aagccttgct caaagacatg cttgttaaga aatgggggcg
     2281 aatagtaaat atcgtctcac tttcgggtat taagggaatg cccgggcagg caaactattc
     2341 ggctgccaaa ggtgggcttg tagccgccac aaaggccttg gctcaggagg ttgctaaaaa
     2401 gcgggttacg gtaaatgccg tggctccggg tttcataaaa accgatatga ctgccgattt
     2461 gaacgaagcg gaactaaaga gtcttattcc cgccggacgt tttggagagc cgagcgaagt
     2521 ggcagaactt gtggcttttt tagtttccga atcagcagcc tatatcacgg gcgaggttat
     2581 atccattaat ggaggaattt atacttaagg agctatggca cagcgagtag taattacagg
     2641 aatgggtata tactcctgca tcgggaaaaa caaagaggag gttttgaagt cgctttttgt
     2701 gggcaagtcc ggtataggca tagtacccga aagacgagaa ttgggctatc gttccgctct
     2761 tagcggcatt ttggaaaaac caaatctgaa agagttatta gaccgccgtc agcgactatg
     2821 cttgtccgaa catggacatt atgcctatgt tgccacctta gaggccttac atcaggcttc
     2881 catatcgccc gattatctaa agttaaacga agtgggaatc ctctacggca acgatagcag
     2941 cgccgaggct gtgatcaacg gagttgatat tatgagagaa aagaaaaata ccgtattggt
     3001 gggctccggc aatatttttc agtcgatgaa ttcgactatc accatgaatc tgtctaccat
     3061 attcaatctt cgtggaatta attttaccgt atcgggtgct tgtgccagcg gctcgcacgc
     3121 cataggcatg ggttatctgc tgataaggca aggtctgcaa gactgcgtta tctgcggtgg
     3181 agccgaagag gtcaacaagt attccgtggg aaatttcgat gctctcagtg ccttttctat
     3241 tcgtgaagat gaacccgcaa aggcatctcg cccctttgac aagaatcgcg atggcctggt
     3301 gccaagtgga ggtgccgcca cattaatcct ggagagttac gactctgccc ggaagcgggg
     3361 agccgcgata ctcggcgagg taataggctt cgggttttca tcgaacggcg accatatatc
     3421 cgtgccaaac atagacggac cggttcgttc gctcaatatg gcaatccgcg atgcaggcgt
     3481 taagccggga gatataaaat acatcaacgc tcatgccact tctactccca tcggagacat
     3541 taatgaagca aaagcaattt tagaggtgtt tggcgagcat aaaccatatg taacatcgac
     3601 caaatcgcag acggggcacg aaatgtggat ggcgggagca agcgaagtta tttactctat
     3661 acttatgatg caaaacaact ttattggagc aaacttaaat tttgaggaac cggatgaggt
     3721 ttcgataaat ttgaatatac cggcaaaaag agtgaatacg gcatttgata tgtttttgag
     3781 caactctttt ggctttggag gcaccaattc atccttgatt ataaaaaaat aaacaacagg
     3841 aaatatgact atcgaagaga ttgttgaaaa agctacaacc atattggcag aagagtttga
     3901 agttgaggag tctgagataa caccggaagc ttctcttaag gagacattgg gcttagacag
     3961 cctcgatttg gtagatgtgg ttgttctcgt agaacagcat tttggtgtta ctctcaaggg
     4021 acaagacttt gtcgggatta aaacattcaa cgatttttac gagcttctga atcgaaaaat
     4081 caatggctaa agaacccgaa aagcagtggg aaggcaaatc cagaggcggt cgttggggct
     4141 atacattttt tatctctacg atcagactgc ttggaattcg ctgtgcctat ttctttttgg
     4201 ccttggttgt gctctatttt ataccttttg caccaaaagc caccaaaagc gtttggcttt
     4261 accataggcg tagaagaaaa cttggagtgc tacgatctct cagagagttg tattgccact
     4321 actacgtatt tggtcaaacc ctgatcgata aagttgcaat gaatgccgga atgacaaatc
     4381 gttacaacta tgtgtttgac aactacgaac gttttttgga cttgctcaac agtggtcagg
     4441 gggtagtgat gattggtgct catgtgggtt gctgggaggc cggagccgga ttttttggcg
     4501 agtatggcaa aaagatcaat atagcgatgt tcgatgtaga gcatcaacag ataaaagagc
     4561 tgctcgaaag caattccagt agcaagaata actacaaaat tattgccatt aaccaggatg
     4621 ccatttcggc cgtgttgcag atgaaaattg cccttaacaa cggagagtat ctttgcttca
     4681 atggcgacag atacgtcgat aaaaacaacg cccgggcagt tgaatttatg aatgccagag
     4741 cgaatttccc tatcggacca tttcggattg ctgcaaaatg cggagcacct gtggtgtttt
     4801 actacgcgat gcgcgaaccg gctcgcactt acaggtttat attcgaggaa gctgggattg
     4861 agggcaaaaa ttccgaacaa aatattacag atcagtatgt taagtcgtta gaaaggatac
     4921 tgaaaagcta tcctcgccaa tggtttaatt tctatgattt ttggaacgaa agtattatag
     4981 tataagatat gaaactctct aaaccacttg aagatatata cggcaaagag caacagacgg
     5041 ctctcggagc acagaggaga gcgcaggaga ttgcttttgc acctatggtt tttcaagtct
     5101 ctcgcctgat ggtgaaattt ggaattttag actatttggc aaagagtcgt aacggagcta
     5161 ctcagcaggc gatagtggag cacacgggct tatcgaaata tgccatacag gtgttgttgg
     5221 aggcatcgct ctccataggc acggtattaa taaaacaaga taggtacgag atttctaaag
     5281 caggccggtt tttgctaaac gacccgatgg ccagggtaaa tatgaacttt aatcaagacg
     5341 tcaattattt aggacttttc catttggaag aagctcttag agagggaaaa cctgcagggc
     5401 tgaaagtttt tggcaattgg gctacaatct acgaggctct gtctgaactg cccgaacatg
     5461 ttcagaagag ttggtttgcc tttgaccatt tctactcgga tgaatcgttt gcagctgcct
     5521 tggaagtggt gttcggttca aacagaaaga ttaatcgttt gctcgacgtg ggcggcaata
     5581 ccggtcgctg ggccctgcaa tgtgtggact acgataaaga tgtggaggta acaattatgg
     5641 atctgccaca gcaattggag atgatgcgca agaatatagc ggga
//
LOCUS       DAOSGL010000124         5650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12647_length_5650_cov_3.885433, whole genome shotgun sequence.
ACCESSION   DAOSGL010000124 DAOSGL010000000
VERSION     DAOSGL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12647_length_5650_cov_3.885433"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..234
                     /locus_tag="PKY68_04995"
                     /pseudo
     CDS             1..234
                     /locus_tag="PKY68_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
     gene            482..2353
                     /locus_tag="PKY68_05000"
     CDS             482..2353
                     /locus_tag="PKY68_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920374.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOH24429.1"
                     /translation="MNNNAGFTTLDYIIFIAYAVMIVGLGLWLSRSKKGEERDSKDYF
                     LAGGKLSWWAIGASLIAANISAEHFIGMSGSGYRIGLGIAAYEWLAAITLILVAKYLL
                     PIMLDRKIFTIPQLARERYGKGVSFFFSFFWLLVYVFVNLTSVAWLGALAMNQILGIP
                     QMYGVIGLLLFSGLYSIYGGLKAVAWTDVLQVIFLVGGGLITAWFALAAVSGDANSAI
                     EGFKVIFTKIKETEGDLHFSMIIDQNLDGERFKELPGLAVIFGAMWLTNLGYWGFNQY
                     IIQKGLAAKNLKEAKKGLIFAGYLKILIPLIVVIPGITAFVMFQHQADFPGLQETLSG
                     TIGRADDAYPWLLRNFAPVGIRGLAFAALVAAVVSSLASMLNSTSTIFTMDIYKQQIN
                     KNASEKQLVLVGRLTALIALIVAVVSAKPLLGGMDQAFQYIQEFTGFIYPGVVVVFGM
                     GLLWKRATNTSALWTTIATIPVGIIMKIALPELPFILRMGYVCMILISMAVIITFLDK
                     KNRVPADPISPEKRKGLLKAGWFFAITAGITLICAVVFGTNWFGLSLRHIGFHSILLL
                     TFLLGFLAIMMFTNANSVQKDAKSYEMNTELFKTDTGFLLGAMGIVVIIAALYAVFW"
     gene            2441..3130
                     /locus_tag="PKY68_05005"
     CDS             2441..3130
                     /locus_tag="PKY68_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOH24430.1"
                     /translation="MDSFYKEHPRFFVSVDCLVFGFANGQLNLLVCKRKMEPVQGTWS
                     LPGGFIEQDESAEAAAERVLSNLTGLEDIYMEQLKMFSQPKRDPGERVLSVAFYALID
                     LGQEALEIKPSYQAHWFNINELPDLIFDHPEMVDLALKTLRHAVSTKPIVFNLLPEKF
                     TLPQMQTLFEAIYDQALDKRNFRKKVQSMPFLVKLNEKDKQSSRKGAFLYTFDQKEYE
                     RASQNGFCFSL"
     gene            3176..3862
                     /locus_tag="PKY68_05010"
     CDS             3176..3862
                     /locus_tag="PKY68_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOH24431.1"
                     /translation="MELSLLKEKVFQANLDLVKHGLVIFTWGNVSAIDREKGLVVIKP
                     SGVAYEDMRVEDMVVLDLNGKKLDGKLKPSSDTATHLELYKAFPEIGGVVHTHSTFAT
                     AWSQAGMSIPNIGTTHADYFSREIPCTRDMDEEEVMGEYEKQTGLVIVEAFKDINPVQ
                     VPGVLVKNHGPFAWGKSADDAVHNAVVMEQVAKMAYIAFSINPELTMNPLLIQKHFFR
                     KHGPGAYYGQ"
     gene            3903..5405
                     /gene="araA"
                     /locus_tag="PKY68_05015"
     CDS             3903..5405
                     /gene="araA"
                     /locus_tag="PKY68_05015"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOH24432.1"
                     /translation="MNYRDLEVWFITGAQLLYGGDAVVSVDAHSNEMVKGLNDSGRLP
                     IKVVYKGTVNSSDEISAAFRAANADTGCIGVITWMHTFSPAKMWIHGLMDFKKPLLHL
                     HTQYNEKIPWDTMDMDFMNLNQSAHGDREFGHITSRLRKPRKVVVGYWKDKATQDKIA
                     VWMRVCAGWADAQNMRIIRFGDNMNNVAVTDGDKVEAEIRLGYHVDNAPIAKLVPYVK
                     AVTEAEIDALVAEYDKLYDIAPDAAKGGKNHIHLRAAAAQEIGLRRFLDDKGAKAFTT
                     NFDELEGMDQLMGFASQRLMAEGYGFGAEGDWKTAALVRTMYVMSRGLEGGGSFLEDY
                     TLNFDGENTSVLQAHMLEISPAISKAKPKIEVHFLGIGDAGTCARLVFTAHEGEGIAC
                     TIVDIGNRFRMIVNDVKVIKPKALPKLPVARALWIPQPNFEVGAGAWILAGGTHHSSF
                     SFALTKEYMEDYAEIADIELLLIDKDTTIRQFKKDMRINEVYYMLNKALR"
     gene            5416..>5650
                     /locus_tag="PKY68_05020"
     CDS             5416..>5650
                     /locus_tag="PKY68_05020"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610398.1"
                     /note="catalyzes the phosphorylation of ribulose to
                     ribulose 5-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOH24433.1"
                     /translation="MKKYVIGLDYGSDSCRALVVDAHTGEELGTSVRYYPRWAEGKYC
                     DPIINQYRQHPKDYLECLEASVKEALADSGPGVA"
BASE COUNT         1505 a         1228 c         1348 g         1569 t
ORIGIN      
        1 aaaagcggac gcatactcaa ggtgttcacc tcggaacccg gagtacaagt atacaccggt
       61 aatttcctgg atggcagtct gaggggcaaa aatggtgttg tctataatca acgtgctgct
      121 atctgtcttg aaacccaaca ttatcccgac agtcccaaca aggccgactg gccatcggta
      181 gtgttgcgtc ccggtgaaaa atacaccagc cattgcatct atcaatttgc ggtaaaatgc
      241 agcaacgagg gtaaagagtg ttgcggcaaa aacaagaaat gctgcaaaga gggggccgat
      301 tgtaaggaag ggggtgcctg ctccgattct tgctgccaaa aaccctgcgg aaaggccgaa
      361 ggaaaaccct gtgccggctc tgaagcaaag cctggccgga acagaaacaa agctctacaa
      421 taaggaataa ataagatatc actttttaaa aaccttaata ttaaccaatt aattttttgt
      481 tatgaataat aacgctggtt ttacaaccct cgactacatc atctttatag cgtacgctgt
      541 tatgattgta gggctcggtc tttggctttc acgctccaaa aaaggagagg agagagactc
      601 caaggattat tttcttgccg gtggcaagct ttcctggtgg gcaataggtg cctctctgat
      661 tgcagccaac atttcggctg aacattttat cggaatgtcg ggttccggtt atcgtattgg
      721 tttaggtatt gccgcttatg agtggctggc agccatcacc ttgattttgg tggcaaaata
      781 cctgctgccc atcatgttgg acaggaaaat tttcaccata cctcaactgg cccgtgaacg
      841 ttacggaaaa ggggtttcct tctttttctc cttcttttgg ctcttggttt acgtctttgt
      901 caacctgacc tccgtagctt ggttgggagc gctggctatg aatcagattt tgggcatacc
      961 ccaaatgtat ggagtaatcg gcctgttgtt gttttccggt ctttattcca tttacggcgg
     1021 tctgaaagcg gtagcctgga ccgatgtgtt gcaagtgatc ttcctggtag gtggtggcct
     1081 tattaccgct tggtttgctc tggctgccgt cagcggcgat gccaacagtg ccatagaagg
     1141 tttcaaagta atattcacta aaattaaaga aaccgaaggc gacttgcatt tcagcatgat
     1201 tatcgaccag aaccttgatg gagaacgctt caaagaattg cccggtctgg ctgttatttt
     1261 cggcgccatg tggcttacca atttaggtta ttggggattc aaccagtaca ttattcaaaa
     1321 aggattggct gctaaaaact tgaaagaagc caaaaaagga ttgatttttg ccgggtacct
     1381 caaaatattg attcctctta tcgtggttat ccccggcatc actgcctttg tcatgtttca
     1441 acatcaggca gactttcccg gtttacagga aacactttca ggcaccattg gaagggcgga
     1501 cgatgcctat ccttggttgt tgcgtaactt cgcccctgta ggtattcgcg gtctggcatt
     1561 tgccgccttg gttgcagccg tggtctcttc actggcttcg atgctgaatt ccacttcgac
     1621 cattttcacc atggacatct acaagcaaca gatcaacaaa aacgccagcg aaaaacaatt
     1681 ggttctcgta ggtcgtttaa ccgcgttgat tgctttgatt gttgccgtcg tttcggctaa
     1741 acccttgttg ggaggaatgg atcaggcttt ccagtatatc caggagttta cgggctttat
     1801 ttatccgggc gtggtagtag tgtttggcat ggggcttctt tggaaaaggg caaccaacac
     1861 atctgcttta tggactacca ttgcgaccat accggttggc attattatga aaatagcctt
     1921 acctgagttg ccttttatcc ttcgtatggg ttatgtctgt atgattttga tttcaatggc
     1981 cgtaatcatc actttccttg acaaaaagaa cagggttcct gccgatccga tatcgcccga
     2041 aaaacgcaag ggtttgctaa aagccggttg gttctttgcc atcactgccg gtattactct
     2101 tatctgcgca gttgtttttg gaacaaattg gtttggtctc agcctcagac atatcggttt
     2161 ccactctatc ttactgttga ccttccttct gggcttcctt gctattatga tgtttaccaa
     2221 tgccaattcc gtccaaaagg acgcgaagtc ttacgaaatg aataccgaac tattcaagac
     2281 agacactggc ttcctgttgg gagctatggg aatagtcgtc atcattgccg ctctatacgc
     2341 cgtattctgg taaatctttt tcaagtgaac ccggtttttg ccgggttcac ttgattatta
     2401 tataaagctt atccgatttt atacctacgt agttgaaggt atggattcat tttataaaga
     2461 gcatccccgt ttttttgtat ccgttgattg tctggttttt ggctttgcaa acgggcaatt
     2521 gaatctattg gtgtgcaaac gcaaaatgga acctgttcag ggtacctggt ctctgccggg
     2581 aggatttatc gaacaagacg agagcgccga agctgccgct gaacgagtct tatccaatct
     2641 gaccggactc gaagacattt atatggagca attgaaaatg tttagtcagc ccaaacgaga
     2701 tccgggagag agggtgcttt ccgttgcttt ttatgccttg atcgacctgg ggcaggaggc
     2761 tttggaaata aaaccatctt atcaggcaca ttggttcaat atcaacgaac tgcccgactt
     2821 gatttttgat catccagaaa tggttgatct tgctctgaag acattgagac acgctgtatc
     2881 aaccaagccg atagttttca atctcttgcc ggaaaaattc acactccctc aaatgcaaac
     2941 tttgtttgaa gcaatctatg accaagcttt ggataagcgc aattttcgca agaaagttca
     3001 gtccatgccc tttttagtta aattgaatga aaaggacaaa cagagttcaa gaaagggagc
     3061 ctttttatat acctttgatc aaaaagagta cgaaagagca agtcaaaacg gcttttgctt
     3121 cagcctgtaa gtgatttaac ttataaaaat tataagaaca tcgaatttta actctatgga
     3181 attgtcgtta ttaaaagaga aagtatttca ggccaacctt gatttggtta agcacggcct
     3241 ggttattttt acctggggca acgtgagtgc cattgacagg gaaaaaggac tggtggtgat
     3301 aaaaccttcg ggagtagctt acgaggatat gagggtcgaa gacatggtag tgctcgatct
     3361 gaatgggaaa aagctagatg gtaagctcaa accctcgtcc gatacggcca ctcacctgga
     3421 attgtacaaa gcttttcccg aaataggcgg tgtggtgcat acccattcta cttttgcaac
     3481 tgcctggtct caggccggta tgtccattcc gaatataggc actacccatg ccgattattt
     3541 cagcagagaa ataccctgta cccgcgacat ggatgaggaa gaagtgatgg gggaatacga
     3601 aaaacaaacc gggttggtta ttgtagaagc cttcaaagac atcaatccgg ttcaggtgcc
     3661 gggggttttg gttaaaaacc atggtccttt cgcctggggc aagtcggctg acgatgccgt
     3721 acacaacgcc gttgtgatgg aacaggtagc caaaatggct tatatagcct tttcaatcaa
     3781 tcctgaattg accatgaacc ctttgcttat tcaaaaacat ttctttcgca aacatggccc
     3841 cggtgcctat tatggtcaat gaacaataga aaacagtatt aataaataaa aatttcacta
     3901 ctatgaatta tcgcgatctt gaagtatggt tcattaccgg tgcacaattg ttgtacggtg
     3961 gtgatgcagt tgtgtctgtt gatgcacatt caaatgaaat ggtaaaaggt ttgaacgact
     4021 ccggcagact gcctattaaa gtggtttata aaggcacagt caattcgtcc gatgaaatat
     4081 ctgctgcgtt cagagctgct aatgccgaca ccggttgcat aggtgttatt acatggatgc
     4141 acactttttc tcctgccaaa atgtggatac acggcctgat ggattttaag aagcccttgc
     4201 tgcatctgca tacccaatac aacgaaaaga taccttggga taccatggac atggatttca
     4261 tgaatctgaa tcaaagtgca cacggagacc gtgaatttgg gcatatcacc agtcgtcttc
     4321 gcaaaccccg taaagtggtg gtcggctatt ggaaagacaa agctactcag gacaaaattg
     4381 ccgtctggat gcgtgtttgt gcaggttggg ccgatgctca gaatatgcgt atcattcgtt
     4441 ttggcgacaa tatgaacaac gtggccgtga ccgacggcga caaagtggaa gccgaaatac
     4501 gcctgggtta ccatgtagac aatgctccca ttgctaaact agtaccttat gtcaaagccg
     4561 tcactgaggc cgaaatcgat gctttagtgg cagaatacga taaattatac gacattgctc
     4621 ccgatgcagc caagggcggt aaaaaccaca tccatttgcg tgccgctgcc gcacaggaaa
     4681 ttggcctgcg ccgtttcttg gacgataaag gtgccaaagc ttttactacc aattttgatg
     4741 aactggaagg catggaccaa ctcatgggtt ttgcttctca acgcctaatg gccgaaggtt
     4801 acggtttcgg tgccgaaggt gactggaaaa cagctgcttt ggtcagaacc atgtacgtta
     4861 tgagccgggg cctggaaggc ggcggttcct tcctggaaga ctacacactg aattttgacg
     4921 gcgaaaatac ctctgtgctt caggcccata tgttggaaat cagtcccgct atttcgaaag
     4981 caaaacccaa aatagaggtg catttcctgg gtatcggcga tgcaggtacc tgtgcacgtc
     5041 tggtctttac tgctcacgaa ggcgaaggaa tagcctgtac catagtagat ataggcaatc
     5101 gtttccgtat gatcgtaaac gacgtgaaag tgatcaaacc taaggctttg cctaaactgc
     5161 ccgtggcaag agcgctctgg attcctcagc ccaacttcga agtgggagcc ggagcctgga
     5221 tattggccgg tggtactcac cattccagtt tttctttcgc gctcaccaaa gaatatatgg
     5281 aagattacgc cgaaattgcc gatattgaat tgctcttgat tgacaaagac acaaccattc
     5341 gtcagttcaa gaaggatatg cgtatcaacg aagtctatta tatgttgaac aaagctttac
     5401 gctaaattat ttattatgaa aaaatacgta ataggactgg attacggaag cgactcatgt
     5461 cgtgctctgg tggtcgatgc ccacacgggt gaagaattgg gtacctctgt acgctattac
     5521 cccaggtggg ccgaagggaa gtattgtgat ccgataatca accagtaccg tcaacatccc
     5581 aaggactact tggagtgtct ggaggcttct gtcaaagagg ctttggccga tagtgggccc
     5641 ggcgtggccg
//
LOCUS       DAOSGL010000125         5649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12649_length_5649_cov_4.687344, whole genome shotgun sequence.
ACCESSION   DAOSGL010000125 DAOSGL010000000
VERSION     DAOSGL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12649_length_5649_cov_4.687344"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..661
                     /locus_tag="PKY68_05025"
     CDS             224..661
                     /locus_tag="PKY68_05025"
                     /inference="COORDINATES: protein motif:HMM:NF023021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3244 domain-containing protein"
                     /protein_id="HOH24434.1"
                     /translation="MNKLVLSLLFLGIAFSAFSQNSDNDEIRLFKKSDPKCYSVPESK
                     PAVQESVTASYNTNNLFISIADFTGAVTVEVLDANNMVVINDTRKVSKQAVLTYSIGK
                     LKPGVYTLVVKASGEYYGEFYKKALDNVVLSRESLILSSLSYQ"
     gene            complement(889..2763)
                     /locus_tag="PKY68_05030"
     CDS             complement(889..2763)
                     /locus_tag="PKY68_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24435.1"
                     /translation="MKNNIKKIAFIAVAISLCTTFNSCSLDEEDPGGFTFENLSQTTT
                     GFQTLVNNIYFGLERSFYGNTNKINFMAITEAQTDLWTTPRNMDNTNQQYFWFYAGGS
                     PNTTYTLSFLYSLYDGIGSCNKVLQYVHIPPYNTEAERNAKAAEARFMRAVYYYHAAE
                     QFGGATIVTEKNTSIDQSVYVPVKNSPQEIYDQIIIPDLIYAIEWLPKGDYTAITRPT
                     KKSAIGFLARAYLQATRWVDDKTDYYRNALTYAKMLIDDWEVSGGANYMTYMYPNYGD
                     IFSEAENFTNKEALWKHAYYSDATYNGSSNGAHVLNLSHTLFRCQLNEFPARENNADT
                     YVTWEGSVNGSFMPTQHLLSLFDNGDGTLDPRFYKIFQNDWNANKDFVWTKDFANKFD
                     KDTTAVVGQAIAVGEAAIEFIMPQDADYAAKKAAKHTSKHLIVDYADVYNDAAKSIFM
                     KHSYVNTTPDYKADGSVENFFSYFYPSLTKHNTTKFYPQNVSRLRFGNTSAMHILRMS
                     EMYLIAAEADLKVNNGANALAYLNKVRSRAGAQNLSGTVTIRTILDERGRELCGEWVR
                     YYDLARTGMLDNATYLTETNPSLAAYFNVDYVLRPFDTSTFLPSLNNDSIYQNDGYGY
                     "
     gene            complement(2786..>5649)
                     /locus_tag="PKY68_05035"
     CDS             complement(2786..>5649)
                     /locus_tag="PKY68_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH24436.1"
                     /translation="VNNQRSLVIVLEVADISLDEVVFIGYGTVKRRDLTGAVSSIGPK
                     EIVLAPTNNIMEALQGRIAGLDITTPSGAIGSNPSVALRGVRSIYGNNDPLLVIDGVI
                     MPTSTIVEDLVVNTGDNSTNMNVTNYLNQLNPADIESIDVLKDAASTAIYGSAGANGV
                     ILITTRKGQAGDAIVNFDSYFSLKGTPLFKHGMQGEEWLQYYKTAYKNANGADLIDIS
                     TLFGGNTYYLDAYNKGKWIDWVDEAVSNGRRATTQKYSLSVSAGGPKSQVYSSLSYTT
                     EQGLMSIESSDQVVFRLNIDQNLFSWAKVGFFTNSTFQMNNTCSPVFQSSVGRLPLGD
                     VYDEYGNLNYYYIGVDAGNGQLSPLADWRKDQYANEQKSVYVQPTAYLQIKPFKDLIF
                     KSQLGGSHANVERGRYYGSQSITQSPHYVGYVAPYAEIYQQNEWSYIWDNILTYDKVF
                     NEDHSITLTGLSSWNYRQISDLYTGSMGQELDAWLFYRMAAGNAFYANSNYKQTQQMS
                     YAGRANYSYKGKYLATASVRYDGVSWLSEGRKWDYFPSMALAWRISDEAFMEDMEDWL
                     SNLKLRLSYGKTGNAGGMTAYSTASGMFKYAQNISVDGPNSPAAGGMTQYTGTYGNAG
                     IGWEKSYTWNVGLDFGLFGNRLEGSFEWYDTRTKDLLYMRAMPVTTGITGWGWTLATW
                     QNLGETMNTGWEFSLNSRNIVRKDFNWTTSLDLAWQKDQILSLPGGDFRDNKLGWFFE
                     GESINSVYAYKYLGIWQQDEAAEAQTYGCEPGAVKIETVEQDGDGGVHIYNANDMQVI
                     GSYIPRVTAGLNNAFNYKNIDLSVYLMGRFGHLVEYSYYSGSASVTGNQPSGVDYWTP
                     DNTDAYYFAPGLNYNPAASAAHFMDGDFIKVKNVTLGYTLPSSLTKKALINRMRIYAT
                     AYNPAVWTKSKQLKGIDPESSSSRYPLYKQFVFGLNLTF"
BASE COUNT         1577 a         1240 c         1208 g         1624 t
ORIGIN      
        1 agaaattgcc acaattttac tcataacaga ggcaaatgtt cgctcaacca agtcgaaaat
       61 caaaaaaaag agcgaaactt tttgatatta ctttattgat atacaagttg ttacagcgaa
      121 actttttggg caactcaaac aattaagcga aattcgttat atttttgaca catcgttttc
      181 ttgtttagtt acttgaatta ttcatttttt aaatttttgc cttatgaaca aacttgtatt
      241 atcattattg tttttgggaa ttgcgttttc agctttttca caaaattcgg ataacgatga
      301 gattcgtctc ttcaaaaaat ccgatcccaa atgttactcc gttccggaat ctaagcctgc
      361 agtacaagaa tctgtgacag cttcttataa caccaacaac ctgttcatca gcatcgctga
      421 ttttaccggg gcggtaacgg tagaagtgct tgacgccaat aatatggtgg taattaacga
      481 tacccgtaag gtatcaaaac aagctgttct gacctattct atcggcaaac ttaaaccggg
      541 agtgtatacc ttggttgtga aagcctcggg cgaatattat ggagagtttt acaaaaaagc
      601 cctcgataat gttgttcttt ccagagaatc cctgatttta agttctttat cctaccaata
      661 gggggcagag tcctgaagga gttcaggatg ttttaaatta aggataaatc aagcaactcg
      721 aagcttcctg cgtcaacaaa tctttcaaat gtgggtgata tttgttgatt cagggagctt
      781 tagttgtatc aaaccactac aaaatcggac aaaaatgccg gttaaatggt aaagaaaaag
      841 aggataactc cgcagagtta ccctcttttt ctttagactc atcaactgtt aataaccgta
      901 tccatcgttc tgataaatgg agtcgttgtt gagtgaaggc aggaatgtgc tggtatcgaa
      961 gggacgaaga acgtagtcca cattgaaata ggcggccagc gaaggattgg tttctgtcaa
     1021 ataagtagca ttgtccaaca ttccggtacg ggcgaggtcg taataacgaa cccattcacc
     1081 gcataattcg cgcccgcgtt catccaatat tgtcctgata gtgacggttc cgctcagatt
     1141 ctgagcaccg gcccgactgc gaactttatt taaataagca agggcattgg ctccattgtt
     1201 gactttcaaa tcggcctcag cagcaatcag atacatttcg gacatacgca atatatgcat
     1261 ggccgaggtg ttgccaaagc gcaatctcga cacgttctgc ggataaaatt tagtggtatt
     1321 gtgcttggtc aacgaaggat aaaaataaga gaagaaattt tcaacactgc catcggcttt
     1381 ataatcgggc gtcgtgttga cgtagctgtg cttcataaat atgctcttgg ctgcatcgtt
     1441 atacacatcg gcataatcca caattaaatg tttgctggtg tgtttagctg cctttttagc
     1501 agcataatca gcatcctgcg gcataataaa ctctatggcg gcttctccta cggcaatagc
     1561 ttggccaacg acggctgtcg tgtctttgtc aaatttattg gcaaagtctt tggtccacac
     1621 gaaatccttg ttggcattcc aatcgttttg aaagattttg tagaaacgcg ggtcgagtgt
     1681 accatcgcca ttgtcaaata aactcagtag atgttgagtc ggcataaagg aaccgttgac
     1741 cgatccttcc caggtaacat aagtatcggc attgttttct ctggccggga attcgtttaa
     1801 ctggcaacgg aataaggtat ggcttagatt caacacatga gcgccgttgc tgctgccatt
     1861 gtaggtagca tctgaataat aggcatgttt ccacaaagct tctttattgg tgaaattttc
     1921 cgcttccgaa aaaatatcgc cgtaattggg atacatatag gtcatgtaat tggcaccgcc
     1981 ggaaacttcc cagtcgtcta tgagcatctt agcataggtc agggcatttc tgtaataatc
     2041 ggttttatca tcgacccagc gggtggcctg caaataagct ctggctaaaa agccaatcgc
     2101 cgacttttta gttggccggg taatagcagt ataatctccc ttaggcagcc attctatcgc
     2161 atagatcaaa tccggaatta tgatttgatc gtatatttcc tggggagaat ttttaaccgg
     2221 aacatacacg ctctgatcta tactggtgtt tttttccgtc actatagtag ctcctccaaa
     2281 ttgctctgcg gcatggtaat aatagacagc acgcataaaa cgggcctcag cagccttggc
     2341 attgcgttcc gcttcggtat tgtacggagg aatatgcaca tattgcaaaa ccttattaca
     2401 ggaaccgatt ccatcgtaca aagaatacaa aaagcttaag gtataagtgg tgttgggcga
     2461 accgcctgca taaaaccaaa agtattgttg atttgtatta tccatgttgc gcggagttgt
     2521 ccacaagtcg gtctgtgctt cggtaatggc cataaaatta attttgttgg tattaccgta
     2581 aaaacttctt tccagaccaa aatagatatt gttgaccagc gtttgaaatc cagtggttgt
     2641 ttgagacaaa ttctcgaaag taaaaccgcc gggatcctct tcgtcaagtg aacaagagtt
     2701 gaaagtggta cagaggctta ttgctacagc aataaaagct atctttttaa tattgttttt
     2761 cataactgta atctcctttc aatgattaaa atgtaagatt aagaccaaac acaaattgtt
     2821 tgtacaaagg atagcgcgaa gaagatgatt ccggatctat tcctttgagc tgtttgctct
     2881 tggtccaaac agccggattg tacgctgttg catagattct catcctgttg atcagcgcct
     2941 ttttggtgag actagaaggc aaagtataac ccaaagtaac attcttgact ttaataaagt
     3001 ctccgtccat aaaatgagcg gccgatgcgg caggattgta attcagtccc ggagcaaaat
     3061 aatacgcgtc ggtgttatcg ggtgtccagt aatccactcc cgaaggttgg ttgcctgtta
     3121 cagaagccga accgctgtaa taactgtatt caaccagatg tccgaaacgt cccatcaaat
     3181 agacgctcaa atcgatgttt ttatagttaa aagcattgtt taatccggca gtaactctgg
     3241 gaatataaga acctatcact tgcatatcgt tggcgttgta tatatgaact ccgccgtcgc
     3301 cgtcctgttc tacggtttct attttgacag cacccggttc gcagccatag gtttgagctt
     3361 ctgcagcttc atcctgttgc caaataccca gatacttgta tgcatacacc gaattaatag
     3421 actctccttc aaagaaccaa cccagtttat tgtctctaaa atcaccgccg ggtagagaca
     3481 ggatttgatc tttttgccag gccagatcta aagaagtggt ccagttaaag tctttgcgaa
     3541 caatgttgcg ggaattcagg gaaaattccc aacccgtatt catggtctct cccaggttct
     3601 gccaggtggc caaagtccag ccccaaccgg tgattccggt agtgaccggc atggcacgca
     3661 tataaagcaa gtctttggtg cgggtgtcat accactcaaa cgatccttcg agcctattgc
     3721 cgaaaaggcc gaaatccaac ccgacattcc aggtatagga tttttcccaa cctataccgg
     3781 cattaccgta agttccggtg tattgggtca tacccccggc agcaggagaa ttaggaccgt
     3841 cgacagaaat attttgtgca tatttaaaca taccggaggc tgttgaatag gccgtcattc
     3901 caccggcgtt accggtttta ccgtaactaa gacgtaattt cagattagac agccagtctt
     3961 ccatatcttc cataaacgct tcgtcgctga tgcgccaagc caaggccata gaggggaaat
     4021 aatcccattt tctaccttcc gacaaccagg atacaccatc gtaacgaaca gaagcagtcg
     4081 cgaggtattt ccctttgtag gaatagttgg cacgtccggc atacgacatt tgctgggttt
     4141 gcttatagtt gctattggcg taaaaagcgt tcccggcagc catcctatag aacaaccagg
     4201 catccagctc ttgccccata gaacccgtat ataaatcact tatctgacga taattccagg
     4261 agcttaaacc ggtgagtgtt attgaatgat cttcgttaaa tactttatcg taggtcagga
     4321 tattatccca aatatacgac cactcgtttt gctggtagat ttcggcataa ggtgctacat
     4381 agcccacata atgcggtgac tgagtaatgc tctgtgaacc gtaataacga ccccgctcta
     4441 cattggcgtg agaaccgccc aattgtgatt taaaaattaa atccttgaaa ggcttaatct
     4501 gaagataggc cgtaggttga acatatacgc ttttttgttc attggcatac tgatctttgc
     4561 gccagtcggc caaaggcgat aattgtccgt tgccggcgtc tacaccgatg taatagtaat
     4621 tcaaattacc gtattcatcg tacacatcac ccaaaggcaa tcttcctact gaactctgaa
     4681 acaccgggct acaggtgtta ttcatttgga aagtactgtt ggtaaagaag cctactttag
     4741 cccaactgaa aagattctgg tcaatattca aacggaaaac cacctgatcg gagctttcga
     4801 tactcatcaa cccctgttca gtagtatagg acaaggaact gtagacctgc gatttgggac
     4861 caccggccga aacagacaaa ctgtattttt gagtggtggc tcttcgtccg ttcgacacag
     4921 cttcgtccac ccagtctatc cacttaccct tgttgtaggc atcgaggtaa tatgtgtttc
     4981 cgccgaagag tgttgatata tcgatcaagt cggctccatt ggcgttttta taggcggttt
     5041 tataatattg cagccattct tctccctgca taccgtgttt gaatagcggg gtgcctttta
     5101 gggagaaata ggaatcgaaa ttgacaatag cgtctccggc ctggcccttt ctggtggtta
     5161 taagtattac accattggct ccggccgatc cgtatattgc ggtcgatgcg gcatctttga
     5221 gcacatctat actttcgatg tctgcaggat taagttgatt caggtagttg gttacattca
     5281 tattggtact attgtcgcct gtatttacaa ctaaatcttc cacaatagta cttgtaggca
     5341 ttatcacgcc gtctataacc aacagggggt cgttattgcc gtaaatagag cggacaccac
     5401 gcagcgcgac actggggtta ctacctattg caccgctggg agttgtaata tccaaaccgg
     5461 caatacgtcc ctgcaaagcc tccattatat tattggtggg tgccagtaca atttctttag
     5521 gaccgatcga ggaaacggct ccggtcaaat cgcggcgttt taccgttcca tagccgataa
     5581 aaacaacttc atccaagcta atatcggcca cttctaaaac aataacaagg gaacgttgat
     5641 tgttaaccg
//
LOCUS       DAOSGL010000126         5612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12776_length_5612_cov_3.853158, whole genome shotgun sequence.
ACCESSION   DAOSGL010000126 DAOSGL010000000
VERSION     DAOSGL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12776_length_5612_cov_3.853158"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKY68_05040"
     CDS             <1..212
                     /locus_tag="PKY68_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24437.1"
                     /translation="IIDPIDWDETVLTANPAYFADKDGLHHSAGLGLKIAMNENFVLS
                     ADFGKAFNKQDGAGLGTYIGLNYLF"
     gene            230..871
                     /gene="pyrE"
                     /locus_tag="PKY68_05045"
     CDS             230..871
                     /gene="pyrE"
                     /locus_tag="PKY68_05045"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOH24438.1"
                     /translation="MKTLKKLIAEKLLKIKAVKLQPSNPFTWAMGWKSPIYCDNRKTL
                     SYPQLRNFIKIELARIIREKYEQVDAIAGVATGAIAQGAMVAEELCLPFVYVRSSPKD
                     HGLENLIEGDLKPGMKVVVIEDLISTGGSSLKAVEAIRKDGSEVIGMLAIFTYGFDVA
                     IERFKQAKVELTTLCDYDTILETALETNYISDADVKTLQIWRKDPANWKPRIQ"
     gene            880..1296
                     /locus_tag="PKY68_05050"
     CDS             880..1296
                     /locus_tag="PKY68_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOH24439.1"
                     /translation="MTEFKTQTGRIAAPACRVFEVLSDWNQLEKIKEKIPESKLRGLE
                     FDADSCRFVIDSYGVGEIALCISERNPCSVIKIKAEQSPIDFNAVISIEEENPEACLL
                     TIGLEAALPFMLKHILSPSIQTGLDKILNLLTEYSY"
     gene            1296..2636
                     /gene="argH"
                     /locus_tag="PKY68_05055"
     CDS             1296..2636
                     /gene="argH"
                     /locus_tag="PKY68_05055"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOH24440.1"
                     /translation="MAMKLWQKNTEVDKDIERFTTGKDREMDLYLAEFDVLGSAAHAR
                     MLEQIGLILPEELPLLLRGLREIYEQIQAGKFTIEEGVEDVHSQVELMLTRKLGDLGK
                     KIHSGRSRNDQVLLDLKLFTRHKLEEVLEAVHALFELLIKLSERYKKVMMPGYTHLQI
                     AMPSSFGLWFAAYAESLVDDVQVLQAAFKICNRNPLGSAAGYGSSFPLNRSLTTQLLG
                     FEGLNYNVVYAQMGRGKMEKTTACALASVAGTIAKLAYDACLFSSQNFGFIALPAEFT
                     TGSSIMPHKKNPDVFELTRAKCNRLQQLPENISAITQNLPSGYFRDYQLLKELFLPAF
                     DELIDCIKMTSASLERIRVNEHILDDPRYEYIFSVEEVNKRVLNGVPFREAYRQVGME
                     IEAGSFRADKNIKHSHEGSLGNLCNDKITARMNELLNSFPFEHIHKALDGLLID"
     gene            2868..5492
                     /gene="clpB"
                     /locus_tag="PKY68_05060"
     CDS             2868..5492
                     /gene="clpB"
                     /locus_tag="PKY68_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOH24441.1"
                     /translation="MNLNQFTIKSQEAVQKAAQKTAALGQQSIETPHLFMGLWEAGEQ
                     TLSFLFNKCGVQPQTVVQAVERQMSSYPKVSGGENYLSKEATAVLQKATAYAAELKDQ
                     FVSLEHILLALLSEKSPVSNILKDAGLRLSELKLAIRELRKGKSADTENAEEVYDALN
                     KYAVNLNEMARNGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIAEGLA
                     HRIVRGDVPENLKTKQVYSLDMGALVAGAKYKGEFEERLKAVVGEVIEAEGEIVLFID
                     EIHTLVGAGKGEGAMDAANILKPALARGELRAVGATTLNEYQQFFEKDKALERRFQPV
                     MVNEPDIADSISILRGLKERYENHHKVRIKDEAVLAAVELSSRYISDRFLPDKAIDLM
                     DEAAARLRLQADSVPEELDEISRRIKQLEIEREAIKREKDELKLNQLGKELADLVEKE
                     TAMKAAWQAEKSHIDRIQELKAEIESLRFEAEKAEREGDYGKVAEIRYGSIQQAEAAI
                     KKESDALKTLQQNGQLVKEEVDAEDIAEVVSRWTGIPVYKMMQSERMKLLRLEEELHR
                     RVIGQDEAINAVADAIRRSRAGLQDPKRPIGSFIFLGSTGVGKTELAKALAEYLFNDE
                     NTMIRIDMSEYQEKFSVTRLIGSPPGYVGYDQGGQLTEAVRHKPYSVVLFDEIEKAHP
                     DVFNILLQVLDDGHLTDNKGRKVNFKNTIIIMTSNLGSELIRERMDQMNESNRDQLME
                     QTREEVFELLKRTLRPEFLNRIDEIIMFKSLTRSEIKEIVRLQLEQLKSNLAERGLRM
                     RYTDKLLDHLAKEAYHPQFGARPVKRLIQRELLNSLSKALLSGSIQEDSLVEIDESGG
                     EILFRSIINPESQGSYSA"
BASE COUNT         1650 a         1216 c         1396 g         1350 t
ORIGIN      
        1 atatcattga tcccatcgat tgggatgaaa cggttttgac ggccaatcct gcgtattttg
       61 cagacaagga cggtttgcat cactcagccg gtctgggtct caagattgcc atgaacgaaa
      121 atttcgtact ctcggccgat tttggcaaag cattcaacaa acaagacggg gcaggacttg
      181 gaacttatat aggcctgaat tacctgttct aattacataa atccttaaga tgaaaacatt
      241 gaaaaaacta attgcggaaa aattattgaa aattaaggct gtcaagttgc agccttccaa
      301 tccctttacc tgggctatgg gctggaaatc gcctatttac tgcgacaatc gtaaaactct
      361 ttcatatcct caactcagga attttatcaa aatagaactg gctcgcatca ttcgtgaaaa
      421 atacgaacag gtggatgcca ttgccggtgt agccaccgga gccattgctc agggcgctat
      481 ggtagccgaa gaactgtgcc tgccttttgt ttacgttcgt tcttcgccca aggatcacgg
      541 cctggaaaac cttatcgaag gtgacttaaa gcccggcatg aaggtggttg ttatcgaaga
      601 cttgatttca accggcggca gcagtctcaa agcggtggag gctatacgca aggacggcag
      661 cgaagtaata ggaatgctgg ccatcttcac ttatggattc gatgtggcaa ttgagcgttt
      721 caagcaagcc aaagtggaat tgaccacttt gtgtgattac gataccattt tagaaacggc
      781 cctggaaaca aattatataa gcgatgccga tgtaaagact ttgcaaatct ggcgcaaaga
      841 tccggctaac tggaaaccca ggatacaata gagaaaccga tgaccgaatt taagacacag
      901 accggtagaa ttgcagcccc ggcctgcagg gtttttgagg ttctttccga ttggaatcag
      961 ctcgaaaaga tcaaagaaaa gatccctgag agtaaattga ggggccttga attcgatgcc
     1021 gactcctgtc ggtttgtgat agacagctat ggtgtgggcg aaatcgcctt gtgtatctcg
     1081 gaacgaaatc cctgttctgt gatcaagata aaggcagagc aatcgcccat cgattttaac
     1141 gcggtcatca gcattgaaga agagaatccc gaagcttgcc tgttgacaat cggcttagag
     1201 gctgctcttc cttttatgtt gaaacatatt ttgtcgcctt ccattcaaac cggcttggac
     1261 aaaattctta acttattgac cgaatactca tattaatagc tatgaaactt tggcaaaaaa
     1321 ataccgaagt agataaagac atcgaacgtt ttactaccgg taaagatcgg gagatggatc
     1381 tttatctggc cgaatttgat gtgttaggct ctgcggcgca tgcgcgaatg ctggaacaaa
     1441 tcggactgat tttacccgaa gaacttcctt tgttgctgcg aggactacgg gaaatatacg
     1501 aacaaataca ggccggcaag ttcactattg aagagggtgt ggaggatgta cactcccagg
     1561 tagaacttat gcttacccgt aaactgggag atctcggaaa aaaaattcac agcggacgtt
     1621 ccagaaacga ccaggtcttg ctcgatctga agctttttac ccgtcacaag ctggaagagg
     1681 ttctcgaagc cgttcatgcc ttgtttgagc tgcttataaa attgagcgaa cgttacaaaa
     1741 aagtaatgat gccgggctac acgcatttgc aaatagccat gccttcttct ttcggactct
     1801 ggtttgcagc ttacgccgaa tcgcttgtag acgacgtaca ggtgttgcag gctgctttta
     1861 aaatatgcaa ccgcaatcct ctgggctcgg ctgccggcta tggttcttct tttcctttga
     1921 acagaagcct taccacccaa ttgttgggtt ttgaaggctt gaactacaac gttgtgtacg
     1981 ctcaaatggg cagagggaaa atggaaaaga ccacggcttg cgctctggct tcggtggcag
     2041 gtaccattgc caaactggct tacgatgctt gtttgtttag ctctcagaat ttcggtttta
     2101 ttgccttgcc cgccgaattc accaccggtt cgagcatcat gccccacaaa aagaatccgg
     2161 atgtatttga attgaccagg gctaaatgca acagactgca gcaattgccc gaaaatattt
     2221 cggccatcac tcaaaatctg ccttcgggtt atttcagaga ttatcagttg ctcaaggagc
     2281 tttttctacc tgctttcgat gaactcatag attgcataaa aatgacctca gccagtctgg
     2341 agcggattcg ggttaatgaa cacatacttg atgatccgcg ctatgaatac atcttcagtg
     2401 tggaagaagt caacaagaga gtcttgaacg gtgttccctt cagggaagct taccggcagg
     2461 taggtatgga aattgaagcc ggaagcttca gagccgacaa gaacattaag cacagccacg
     2521 agggaagcct cggcaatttg tgtaatgaca aaattacagc tagaatgaac gagttactga
     2581 attcttttcc gtttgaacat atacataaag ccttagatgg tttgttaatc gattgattaa
     2641 taagcttata ttttggcttg tttgacataa aaatgaacat cccgcttgtt tttgcgggat
     2701 gtttttttgt gtcaatatga cagtggtgtg tgtttggcaa attctttgaa gatagtaggg
     2761 tgttgtaaaa tatgtaggct gaaataaaac tcaagctgaa gaaagaaggt agaagctgag
     2821 cgaaatggaa gcttttttat aaaaaacgta aaacaaaaat ttgccatatg aatctgaatc
     2881 aatttactat taaatcacag gaagctgtac aaaaagcggc gcaaaaaacg gccgctttgg
     2941 gccaacaaag catagaaacc ccgcatttat tcatgggttt atgggaagcc ggagagcaga
     3001 ccttgtcttt tttattcaac aaatgcggtg ttcaacctca gactgttgta caggctgttg
     3061 aacgccagat gtcttcttat cccaaagtaa gcggcggcga aaactatctc tccaaagagg
     3121 ccacagccgt gttgcaaaaa gccacggctt acgcggctga attaaaggat cagtttgtat
     3181 cgctcgagca tattttgctg gctttgctca gcgaaaaaag cccggtttcc aatatactca
     3241 aagatgccgg cttaagactt agtgaactta agttggccat tcgtgaattg cgaaagggta
     3301 aatcggccga caccgagaat gccgaggagg tatacgatgc cctgaacaaa tacgccgtca
     3361 acctgaacga aatggcccgg aacggcaaac tcgatccggt tatcggcagg gacgaggaaa
     3421 ttcgccgcgt attgcaaata ttgagtcgac gtaccaaaaa caaccctata ttaattggtg
     3481 aacccggaac gggaaaaacc gccatagccg aaggtttggc tcatcgcatt gtgcgcggag
     3541 acgtacccga aaacctgaaa accaagcagg tttattcgct cgatatgggc gctctggtag
     3601 cgggggccaa atacaaggga gaatttgaag aaaggctgaa agccgtagtg ggagaagtga
     3661 tcgaagccga aggagagata gtgctcttta tcgacgaaat tcacaccttg gtgggcgcag
     3721 gcaagggcga aggggccatg gatgccgcca atatcctgaa acctgctctg gccagggggg
     3781 aactccgggc tgtcggagcc accactctga acgaatacca gcaatttttc gagaaagaca
     3841 aagccctcga aaggcggttt caaccggtga tggtgaatga gccggatatt gccgattcca
     3901 tttccatact cagggggctc aaggaacgtt acgaaaatca ccataaggtc agaatcaagg
     3961 acgaagccgt gctggcagcc gtagaattgt cgtcgcgcta cattagtgat cgttttctgc
     4021 ccgacaaagc catcgacttg atggacgaag cggcagcccg cctgcgattg caagcggatt
     4081 cggttcccga ggaactcgac gaaatttccc gccggatcaa gcaattggag attgagcgcg
     4141 aagccatcaa acgcgaaaaa gacgagctta agctcaatca gttgggcaaa gaactggccg
     4201 atctcgtcga aaaggaaacc gccatgaaag cagcctggca agcagagaaa tcacatatag
     4261 accgcattca ggaacttaaa gcagaaatcg agagtttgcg ttttgaagcc gaaaaggccg
     4321 agcgcgaagg cgattacggc aaagtggccg aaatacgcta tggctccata cagcaggccg
     4381 aggccgccat caaaaaggaa agcgacgcac taaaaactct gcagcaaaat ggacagcttg
     4441 tcaaagaaga agtcgatgcc gaggatatag ccgaagtggt ttctcgctgg acaggcattc
     4501 cggtgtataa aatgatgcag agcgaacgta tgaaattgct gcgtctcgag gaagaattgc
     4561 atcgcagggt aataggtcag gacgaagcca ttaacgccgt agccgatgct atacgccgaa
     4621 gcagagccgg cttgcaggat cccaaacgcc ccataggctc ctttatcttt ttgggcagta
     4681 ccggggtagg taagacagaa ttggctaaag ccctggccga atatttgttc aacgacgaaa
     4741 atacgatgat acgaatagat atgtcggaat accaggaaaa gttttcggtt accaggctca
     4801 tcggttcccc tccgggttac gtgggttacg accagggcgg acagttgacc gaagcagtaa
     4861 ggcacaaacc ttattcggtg gttttgttcg acgaaatcga aaaagctcac cccgatgtct
     4921 ttaatattct gcttcaggtg cttgatgacg gtcatttgac tgacaacaaa ggccgtaagg
     4981 tgaatttcaa aaatacgatt ataattatga cctccaacct gggttcggaa ctgatacggg
     5041 aacgtatgga tcagatgaac gaaagtaacc gggatcaatt gatggagcag actcgcgaag
     5101 aagtattcga attattaaag cgaaccctga gaccggagtt tttaaaccgc atagacgaaa
     5161 ttattatgtt caaatccctg actcgctcag agattaagga aattgtgcgc ttgcaactgg
     5221 agcaacttaa gagtaacctg gccgagagag gcttgcgtat gagatacacc gacaaactgc
     5281 tggaccacct ggccaaggaa gcttatcatc cgcaattcgg cgctcgtccg gttaaaaggc
     5341 tgattcaaag agaattgttg aattctttat ctaaggcttt attatcgggc agcattcagg
     5401 aagacagtct ggtcgaaatc gacgaaagcg gaggcgaaat tcttttccgc tcgattataa
     5461 atccggaatc acagggcagc tattcagcat aagacgctga cggcagtgag tttttacacc
     5521 acacgcagca ctgagccagc ggtagctatt aagcataagg caatgccggc agggtagctg
     5581 agagcatcat tgagaaattg cccaaaaaac ct
//
LOCUS       DAOSGL010000127         5600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12814_length_5600_cov_4.181966, whole genome shotgun sequence.
ACCESSION   DAOSGL010000127 DAOSGL010000000
VERSION     DAOSGL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12814_length_5600_cov_4.181966"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /locus_tag="PKY68_05065"
     CDS             complement(<1..2229)
                     /locus_tag="PKY68_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24442.1"
                     /translation="MKRPTKLLTALLALFIAFPSLKAGNMQVYTAGDLPTSAGTFVAP
                     DFFGGAGWSYRSRTMDPAKKDTAFFPNVLGVTDGVAYGIEISKTQDDPGNIFLSLNLH
                     SCDSIIVAGWGTGGRGILVTNSANTDSSWKGASSYNLIQVPVKVGTDSPVEIKITPTG
                     KDTKNSTSNGGTWITRIVVFAKEGSEVTQYYLSNPGFESPDTTLLVTTKNSGIWIPKG
                     WTVDYPTFNEWDQGIVTSLNDAGGSALPLIAQEGSNFYYGRLRWATNLISLKQDMINP
                     PAGYNVVSMEVASPDGYKAQLAVSTDADTVTESPTGTGWETIDLVFAGEEGKNATFSA
                     TASSASGAAQNRMFLDDARMVAYGTNFPPSVLDQRTALIAEANALYGDGSGEGAAALQ
                     EAINTFEAVPTTCALGPAETMMAALVALQTAGAELQAAMYDFNVANASPTNPMDLTSK
                     VVNPSANQGRTGWNWSFSSAVQNQGIGTDALFTKATPPYFEAWTPAANKPMTGRIYQI
                     ITDLPNGKYELTVAAQGKGGATDNLHVYANDALSGIFVDTVITVPFMVTAGTAEIGIK
                     AEAADVNWIAIDEIHLSYLGFDPAAMIDALNAQIAEADSLLTLKMKESVATELTNART
                     NAAAAVAASPAVKSQLEAAGAEMAAALPAANASIAGYATLKAAIEASEAKYNAEAPGA
                     ADFRAAIDAAQAAWDAAKIDPAVQITALAVANNAFDFAAASETNPVVTNLVVNPTFDT
                     NTL"
     gene            complement(2573..>5600)
                     /locus_tag="PKY68_05070"
     CDS             complement(2573..>5600)
                     /locus_tag="PKY68_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24443.1"
                     /translation="APYDLVVLSEFPGGASDITKAVKFLVGNKPLLNFKAYAYTISGR
                     WGWANPSNGLYNTEVPVETHAIVGDRFIYHPIFKGLTIGEDNSIQIFDTLHLTRGKTE
                     KDFKGLQGFVTDTYVGPEGFSLASPIGSPNVTCFHEISSNPAAKYMLLAFAAENYANV
                     GEEGLQLVLNAVDYVASPSAFAVPNFNMNATGATVETVEELRAALTYDYSALNLDEIL
                     IQVKDCLDPSGIYLLGSDGMAFPQSFNDLSVKAAPGATPQFWGSFRSNNGMKVGTLRF
                     EGLSWNGGDSALTGFGDENYQPFAVLQPDSVTKAFLVTNCRFENFDYQRIFRSNACAG
                     ALINKLVFENNYFDNMGWNRPAGSHGQSFIQFVNSNNYQLDSLVFHNNIVKNFHGNQL
                     FNVPRNGTTAAPDTLGNFYVGITHNTFYKLGGQGNSDRNFLEYNGTLGGLNGSVNISN
                     NLFIERWSYEKFPQGYLALPATLADGQSLEVKVLNNFFMPDTVLTPRLGGVEPLQVKG
                     ESVVVPVRNNLFAQDLGITTVFEDEQALTISKGSPLYTAGTNRSPLGVTSRYVARQQS
                     GTLRVSNVPELKTALEIAIGGDVIELENCDDPTGVYLLGSSGFAFPQTFGNLTIKAAP
                     EQEPQLFGRLWSSNGMKLDSLIVERLHWNGGDSSYAGFNSEHYAPFQISGPDTIRQAV
                     VVRHSTFRNFDYQPVLRTRNSTGSLVKEWVFTHCLFDNMGWNRKTGIKAFHFIQFEDN
                     TQAELDRFSFTENILKNFHGSQLFNIAISGSPSADSLVVINITNNTFYRLGGNSTDAR
                     QFLGFNRKNAFDNYININDNLFIERWSTANKYPNSNLALFTPDSAQVMVLNILNNFFY
                     PDTVQALPGTGQNLPVTEGDTERMTFARNDLFMSNLFMDKIFEDSEKLLIHVSSPLYT
                     AGTNAGYVGALDSYTTQTGVVGPRLQPAETGKLFTQQGTLFVHVQKTQNMSIYNILGK
                     EILKRQLQEGLNAIEGLQQGQVYLIKLDDRVTKVVM"
BASE COUNT         1239 a         1320 c         1538 g         1503 t
ORIGIN      
        1 cagggtgttg gtgtcaaagg taggattgac taccaaattg gttacaacag gattggtttc
       61 cgaggccgca gcaaaatcga aagcgttgtt ggcaacagcc aaagcggtga tctgtacggc
      121 agggtcaatt ttggctgcat cccaggctgc ctgagctgca tcgatggcag cacggaagtc
      181 ggcggctccc ggagcttctg cgttgtattt agcttccgaa gcttcgatag cggctttcag
      241 tgttgcgtag ccggcaatag aagcattggc tgccggaagt gcggcggcca tttcggctcc
      301 tgctgcttcc agttgagatt taacagcggg agaagctgca actgcagctg ctgcatttgt
      361 tctggcattg gtcagctctg ttgcaaccga ttctttcatt tttaaggtta acaaggaatc
      421 ggcttctgca atttgagcgt tcagcgcgtc gatcatggct gcgggatcaa agcccaggta
      481 actcaagtgg atttcgtcta ttgctatcca gttgacgtcg gcagcttcgg ctttaatacc
      541 tatctcggct gttcctgccg ttaccatgaa aggaacggta attacagtat caacgaaaat
      601 tccggagaga gcgtcgttgg cataaacatg taaattgtct gttgctccgc ctttaccctg
      661 agcagcaacg gttagttcat acttaccgtt gggcaggtct gtgatgattt ggtagattct
      721 gccggtcatg ggtttatttg ccgcaggagt ccaggcttcg aaatagggag gagtagcttt
      781 ggtgaacaaa gcgtctgttc cgatcccttg attctggact gcgcttgaga acgaccagtt
      841 ccagcctgtt ctgccctgat tggcacttgg attgaccact ttggaagtca ggtccatcgg
      901 attggtcggt gaggcgttgg ctacgttgaa atcatacata gcggcctgca gttcagcgcc
      961 tgcagtttgc aatgctacaa gggcagccat catagtttct gccgggccca gagcacaagt
     1021 ggtgggaaca gcttcaaagg tgttaatggc ttcttgcagg gcagcagctc cttcgcccga
     1081 gccgtctccg tagagtgcgt tggcttcagc aatcaaagct gttctttggt ccagcacact
     1141 gggcggaaag tttgttccgt aagccaccat tctggcatcg tccaggaaca tgcggttctg
     1201 ggcagcaccc gaggcagaac tggcggttgc gctaaaagtg gcgttcttgc cttcttcacc
     1261 ggcaaaaacc aaatcgatgg tctcccatcc ggtgccggtg ggcgattcgg ttacagtgtc
     1321 ggcatctgtg cttacagcca gctgggcttt gtatccatcc ggtgaagcca cttccatgct
     1381 gactacgttg tagccggccg gaggattgat catgtcttgc ttcaggctaa tcaaattggt
     1441 agcccagcgt aagcgtccat aataaaaatt ggaaccttcc tgagcgatga gtggcaacgc
     1501 actgcctcct gcgtcgttga gagaggtaac gattccctga tcccattcat taaaggtggg
     1561 gtagtccacc gtccagccct ttggaatcca gattccactg ttttttgttg ttaccaacag
     1621 agtagtatcg ggcgactcaa atccgggatt gctcagatag tactgagtta cctccgatcc
     1681 ttctttggcg aaaacaacaa tgcgtgtaat ccaggtacct ccgttgcttg tagagttttt
     1741 agtgtctttg ccggtgggag tgatttttat ttccaccgga gagtctgttc ctactttaac
     1801 gggaacctga atcaggttgt aagaactggc tcccttccaa ctggagtcgg tattggcact
     1861 gttggtaaca aggattcccc tgcctcccgt tccccagcct gctacgataa ttgaatcgca
     1921 agaatgcagg ttcaggctaa gaaaaatgtt gcctgggtcg tcctgtgttt tgctgatttc
     1981 tataccgtag gctacgccat cggttactcc caaaacattg ggaaagaagg cggtgtcttt
     2041 tttggcggga tccatggtgc ggcttctgta gctccaacct gcaccaccaa agaagtcggg
     2101 tgcgacgaac gtaccggcac tagtaggaag atcccccgca gtatacacct gcatattccc
     2161 cgcttttagc gaaggaaatg caataaacag cgccaatagc gccgtcaata actttgtagg
     2221 tcttttcata aagtttaatt taaaggttaa aaatgtagag tagttgtttt gatttttttc
     2281 agtttttgaa tgcatagatt gatgacaatt ataaatgcgg acttcaacat tgagtttcag
     2341 cccgttgtga atttacggta ttttaatttt tgttctcagc tgcctgtaag ccttattgat
     2401 tcaaaaatca gaaccaaata tacaattaaa tcgtttaaaa aagcagaaca attacaaaaa
     2461 tttaactaat aagccgggac agtgtgaaac tgtttgttaa taaacaagcc cggtacttgg
     2521 aaccgggctt gtgaaaaaga tcgccaataa tcctgttgag agaggccgtt tcttacatca
     2581 ccaccttagt gactctatcg tcaagtttga ttaagtaaac ctgtccctgt tgcagtcctt
     2641 ctatggcgtt gaggccttct tgcaactgcc gttttaggat ttctttgccc aggatattgt
     2701 agatgctcat gttttgtgtt ttttgaacat gcacaaagag tgttccctgt tgggtaaaga
     2761 gtttgcctgt ttcggcgggt tgcagccttg ggccgaccac tccggtttga gtggtgtagg
     2821 aatccaatgc cccgacgtat cctgcgttgg tacctgccgt gtacaggggc gaagagacat
     2881 ggatgaggag cttttcgctg tcttcgaaga ttttgtccat gaataggttg gacataaaga
     2941 ggtcgtttct ggcaaaagtc atcctttcgg tgtccccttc cgttacgggc aggttctggc
     3001 cggttccggg caaggcttgc acggtgtcgg gatagaagaa gttgttgagt atgttcaaca
     3061 ccataacttg agccgaatcg ggtgtaaaga gggctaggtt gctgttgggg tacttgttgg
     3121 ccgtactcca ccgttcaata aagaggttgt cgttgatgtt gatgtagttg tcaaaagcat
     3181 tcttcctgtt gaatcccagg aactgcctgg catcggtgga gtttcctccc aggcggtaga
     3241 aagtgttgtt tgtgatgttg atcaccacca gggaatctgc agagggtgat cccgaaatgg
     3301 cgatattgaa caactggctg ccgtggaagt ttttcaggat gttttccgta aagctgaagc
     3361 ggtcgagttc cgcctgggtg ttgtcttcga actgaatgaa gtggaaagcc ttgatgcctg
     3421 tttttctgtt ccatcccata ttgtcaaaca aacaatgggt aaagacccac tctttgacga
     3481 gcgaacccgt ggagtttctg gttctgagca ccggctggta gtcgaagttc ctgaacgtgg
     3541 aatgccttac cactacggct tggcgtatgg tgtcggggcc ggagatctgg aagggtgcgt
     3601 aatgttcgct gttgaatccg gcgtaggagg agtctccacc gttccagtgc agcctttcga
     3661 cgatcaggga gtcgagcttc atgccgttgg acgaccacaa cctgccaaag agttgcggtt
     3721 cttgctccgg tgcagctttg atcgtgaggt ttccgaacgt ttgagggaag gcaaagcctg
     3781 aactgcccag caggtaaaca cccgtgggat cgtcgcagtt ttccagctct ataacgtcac
     3841 ctccgatggc gatttccaga gccgttttca gctcagggac gttgctcacg cgcagggtac
     3901 cgctttgctg acgagccaca tagcggcttg ttacacccag gggcgaccta ttggtaccgg
     3961 ccgtatacaa gggcgatccc ttggaaatgg tcagggcttg ctcgtcttca aagacagtgg
     4021 taatgcccag gtcttgagca aagagattgt tgcggaccgg aaccaccaca ctttctccct
     4081 tgacctgcag gggctcaacg cctcccaggc gaggtgtcag taccgtatcg ggcataaaga
     4141 agttgttcag caccttaact tccaggcttt gtccgtcggc cagggtagcg ggtagtgcca
     4201 ggtagccctg ggggaacttc tcgtaactcc agcgttcaat aaagaggttg ttggagatgt
     4261 ttacactgcc gttgagtcct cccagcgtac cgttgtattc caggaagttt ctgtcgctgt
     4321 tgccttgtcc gcccaattta tagaaggtgt tgtgggtgat gcccacatag aagttgccca
     4381 acgtgtcggg ggccgccgtg gtaccgttcc ttgggacgtt gaagagctga ttgccgtgga
     4441 agttcttgac aatgttgttg tggaacacca ggctgtccaa ctggtagttg ttggagttga
     4501 caaactgtat aaagctttgt ccgtggctgc cggccggccg gttccagccc atattgtcga
     4561 agtaattgtt ttcaaatacc agcttgttta tcagcgcacc ggcacaggca ttagagcgga
     4621 agattctctg gtagtcgaag ttttcgaaac gacagttggt caccaggaat gccttcgtca
     4681 cagagtcggg ttgcaagacg gcaaaaggtt ggtagttttc gtcgccgaag cctgtcagag
     4741 cggagtcgcc tccgttccac gagagccctt cgaagcgcag ggtacctacc ttcataccgt
     4801 tgttggagcg gaacgagccc cagaattgag gagtagcccc gggagcggct ttgacgctca
     4861 ggtcgttaaa gctctgtggg aaggccatgc cgtcgctgcc cagcaggtag atgcccgacg
     4921 gatcgagaca gtctttcacc tgaatgagta tttcgtccag gttcagggcg ctgtagtcgt
     4981 aggtgagggc agcacgcaat tcttctaccg tttccacggt ggctcccgtg gcgttcatgt
     5041 tgaagttggg cacggcaaag gccgagggcg aagccacata gtctacggca ttgagcacca
     5101 gttggagtcc ttcctccccg acgttggcgt agttctctgc ggcaaaggcc agcaacatgt
     5161 atttagccgc ggggttggag ctgatctcgt ggaagcaggt cacattgggg ctcccgatgg
     5221 ggctggccag ggagaaccct tcgggtccca cgtaggtatc ggtaacaaaa ccctgtagcc
     5281 ctttaaagtc tttttcggtt ttgcctctcg tcagatgcag ggtatcaaag atttgtatag
     5341 agttgtcttc acctatggtc aatcccttga agatgggatg atagataaac ctgtccccta
     5401 cgatggcatg agtttccaca ggaacttcgg tattataaag tccgttgctg ggattggccc
     5461 atccccatct tccgctgatg gtataagcgt aagccttgaa gttgagcaga ggtttgttgc
     5521 ccaccaggaa cttaaccgcc ttggtgatgt cggacgctcc tcccgggaat tcgctgagca
     5581 ccaccaggtc gtaaggagca
//
LOCUS       DAOSGL010000128         5590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12845_length_5590_cov_3.983921, whole genome shotgun sequence.
ACCESSION   DAOSGL010000128 DAOSGL010000000
VERSION     DAOSGL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12845_length_5590_cov_3.983921"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKY68_05075"
     CDS             complement(<1..442)
                     /locus_tag="PKY68_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24444.1"
                     /translation="MKTKNILILFLLMLHIGLVSARSLVIELKDGTKMYYLVSLNENP
                     CLVFDQGSMSIQSDRFTISDVNKFYISETDDPNQLNNPRQEETFRSTGLAAESLHIII
                     DNLNLDQLPKISLFSIDGKSLECSYRFKGKELILETSGLARGTYL"
     gene            836..3349
                     /locus_tag="PKY68_05080"
     CDS             836..3349
                     /locus_tag="PKY68_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24445.1"
                     /translation="MKIAKRRNRGLTSYAMGRSLLIIPVLLILFLSGSCSKNFDHYYQ
                     RPSWLEKNALEVLEKKGNFNHYLKLVDRTLYSKVLRGSGHYTFFAPNDQAFEVWLQEN
                     GYDKVEDIPLSLATDIVAYGLIYNAYEAANLGNTWLSSAEWELGSSFRKQTPSYKTIF
                     REEVDGQKRWTLHSTGSFNESWHNYRYLPIFTNNYFTANGLSAADYNEFYPQTNWNPA
                     NGNVLGAEIITPDIYVENGVVHEINRVIVPPANLDELISIRGKDPSNTAQGWALFKEL
                     LYHKLPDSSYQFILYSDDVDAQYYYNKMYPDSAVGSVHLKTYNSEYIGLPLNLEGYHG
                     PTEGTTSENGAYTLFVPDAAAIKSYLSNTLLKYTDDFNKLPSTVLSTFIASHICDGLV
                     WPSNFKLAKNLVGLEGEFINGRGVLGSDFAGSGITSAQFASNGMVYTINKVVKSGFFE
                     SVYGRLLLDPAYSFMNQVISANYNTTVYQYLLQSPYSGYLNENYTLLLPANDLLENDG
                     FTFDPSNSTFSNASASSVGGVDVVARINRLAASGVFVRTKDYPLDNFNTAPAALGDSY
                     GGYGFAVNYFGDMVRYKNNYLQAVGNIADNDSVHLQKDTVNYANGQFYQLDKLINYSP
                     RNTMGGSSEGWNQGSLVSFVQDYLDKSDKTLFKTYWDTCLVEIEGSINTSAFYTVLIP
                     TNQRMQEAIDAGYLPTIEEVNANLNGELIKAVAFINAHLIIGEVYPDDGLDRIFPGNY
                     ASYKTTTSHKITEAKLDLVLAKSYVNISKGADRNQLTFKAADIEAGSNVLVYGRGSTQ
                     GTQQVIRGLNNSNVMGPRAVLHSLDGFVCFEINQTLLSN"
     gene            3374..>5590
                     /locus_tag="PKY68_05085"
     CDS             3374..>5590
                     /locus_tag="PKY68_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24446.1"
                     /translation="MKNILNKCLLIALLACLPGLIQAQRVLIRGVVTAYEDKQPLPGA
                     YLLLQNSDGRVVANTITDYEGNYSLLSVIKPGDVLVVSYSGFKKQTIPVENREIINVE
                     MREEILELETATVVGRRQISSGMMSISERDLTTAAVRIEMSELEDLAVASIDDALQGR
                     VAGMDMVTTSGAPGAGMSIRIRGTTSINGSSQPYIIVDGFPYETTISEDFDFSTADEE
                     EYSQMLNIAPGDIKEIIVLKDAAATAIWGSKAANGVLQITTKRGGISPPTVTLGSKGT
                     MSKQAKGIPTLNGDQYVGLILEAMMNSGRTFNPESYPELANDANNPYFYHNYGQNTDW
                     FNELTQTGWTQDHNLAISGGSGKAQYRASVGYYNEEGTTVGTGFERITTRLNVDYNVS
                     EKFRFAANMSYNYSETDRAFISYLRSSYDVLSQAYVRAPNMAVYEYNEAGILTGNFFT
                     PASTLQGYWSGSASSIYNPVAMADESMYKMVSNRIIPNLSVIWNPSSVLRYQFDVGFD
                     VMNNKANAFLPVTATGRPWTESTVNRASDSDSEAFVLQTFNKFLYTPKLGDDHSLQLL
                     AGVNTYDKMEKGFAANASNSASPYLTDPSNGARVAGLSGLGISSSSGQNRSINLFAQL
                     HYTLLDRYIFSATLRRDGSSRFGQNHRWGTYPSASLRYRISGEPFMQKLSEQWLDELS
                     LRASYGVNGAQPKGNYASVATYNVFDFEYLGGTGTYQRNLELVDLRWENTTQANIGL"
BASE COUNT         1506 a         1286 c         1345 g         1453 t
ORIGIN      
        1 aaagataagt gcctcgggcc aaaccggagg tttccaatat cagttccttg cccttgaagc
       61 ggtaagaaca ttcgagcgat tttccgtcta tgctgaacaa gctgatcttg ggtagttggt
      121 cgagattcag gttgtctatg ataatgtgga gcgattcggc cgccaggcct gtgcttctga
      181 aggtctcttc ctgccgggga ttgttcagtt gattgggatc gtcggtctcg ctgatataaa
      241 acttatttac atcggagatg gtgaaccggt cggattgtat gctcatactg ccttggtcga
      301 ataccaggca aggattttca ttgagtgaga caaggtaata catttttgtg ccgtctttga
      361 gttcaatgac caggcttctg gccgaaacca aaccgatatg gagcatcaaa agaaaaagaa
      421 tcaaaatgtt tttggttttc atcggaaggg tatttaaata gttatgtatt tcgggttttt
      481 atcgttgggc attggtttga ttaacaaata ttaggtcagt tttgtgaata gaaatatcat
      541 tattcaaaca acagaccatt ccaaaaaatc atacatttgt cataaatgta aatactcaca
      601 ctcagttaac taaaggaaac aagacaaatg taggtatttc ttgtcgtgaa taaaagtaga
      661 attatgccaa aatacgctca ttcttgcata atattgtatc atttatgctt acatttatgc
      721 ttatttaaaa cctgaattta aaacttgaaa ttaaaacgta tccacagaca agcataagcc
      781 catagagatg gcttgacaaa cagaaaatta caattttgta ttaacctgaa ataacatgaa
      841 aatcgcaaaa cgccgcaacc ggggccttac aagctacgca atgggacgga gccttttaat
      901 aatacccgtt cttttgattt tgtttctttc gggatcttgc agcaagaatt ttgaccatta
      961 ttaccaacgt ccttcgtggc tcgaaaaaaa cgctctcgaa gtgttggaga aaaaaggaaa
     1021 tttcaaccac taccttaaac tggtcgacag aaccttgtat tctaaagtac tgcgaggttc
     1081 ggggcattat actttttttg cccccaacga tcaggctttt gaggtctggc tgcaggaaaa
     1141 cggctacgat aaggtggaag acattcccct gagcctggca acagacatag ttgcttacgg
     1201 tttgatttac aacgcctacg aggcggccaa tctgggcaat acctggttga gttcggctga
     1261 atgggagttg ggtagttcgt tccgcaaaca gaccccttcg tacaagacta ttttcagaga
     1321 agaagtggac ggacagaagc gctggactct gcattcgaca ggctcattca acgaaagttg
     1381 gcataattac cgttacctgc ccatttttac caacaattat tttaccgcca acggcctgag
     1441 tgctgccgat tacaatgaat tttatcctca gacgaattgg aatccggcca atggtaatgt
     1501 gctgggagca gaaattatca ctccggatat ctatgtagaa aacggggtgg ttcacgaaat
     1561 caacagggtg atagtgcccc ctgccaatct ggacgagctt atatccatcc ggggcaaaga
     1621 cccttccaac accgcccagg gctgggcttt gtttaaggaa cttttgtatc ataaactgcc
     1681 cgacagttct taccagttta ttttgtacag cgacgatgtg gatgcgcagt attattacaa
     1741 taagatgtat cccgattcgg ctgtcggttc ggttcatctc aaaacataca attccgaata
     1801 catcggtttg ccgctgaatt tggaaggtta tcacggtccc accgaaggca cgacttccga
     1861 aaacggcgct tataccttgt tcgtacccga cgcggcagcc atcaaaagtt atttgagcaa
     1921 caccctgctc aaatacaccg acgatttcaa caaattgccc tcaacggtgc tgagcacttt
     1981 tattgcttcg catatttgcg acggactggt ttggccgtcc aattttaaat tggccaaaaa
     2041 cctggtcggt cttgaggggg aatttatcaa cggtcgaggc gttctgggca gtgattttgc
     2101 gggctccggt attacttcgg ctcaatttgc aagcaacggc atggtttaca ctatcaataa
     2161 ggtggtaaag tccggctttt ttgagtcggt ctatggcagg ttgttgcttg atcccgccta
     2221 ttcctttatg aatcaggtga tatcggccaa ttacaatact actgtttatc aatatttgct
     2281 gcaatcgcct tattcgggct atctgaacga aaactacact ttattgctgc cggccaacga
     2341 tttactcgaa aacgacggtt ttacttttga tccttccaac tctactttct ccaatgcaag
     2401 cgcctcttcg gtggggggag tggatgtggt ggcaagaatc aaccggctgg ccgccagcgg
     2461 agtttttgtg agaaccaagg attaccctct ggataatttc aatacggctc ccgccgctct
     2521 cggcgacagt tacggaggtt acggttttgc ggtgaattat ttcggcgata tggttcgcta
     2581 caaaaacaac tacctgcagg ctgtaggcaa tattgccgat aacgattcgg tgcatttaca
     2641 aaaagacacg gtcaattacg ccaacggtca gttttatcag ttggacaaat tgatcaatta
     2701 cagccccagg aacaccatgg gcggatcgtc cgaaggctgg aaccagggca gtttggtcag
     2761 ttttgttcaa gattatctgg acaagagcga caaaaccctg tttaagacct actgggacac
     2821 ttgtctggta gaaattgaag gttctataaa taccagtgcc ttttatactg tgctgattcc
     2881 cacaaaccag cgcatgcagg aagccatcga cgccggttac ctgccgacta tagaggaagt
     2941 gaatgccaat ctcaatggag agttgataaa agccgttgct tttatcaacg ctcacctgat
     3001 tatcggggag gtttatcccg acgatggtct ggacaggatt ttccccggaa attacgcctc
     3061 ttacaaaacg acgacttcgc acaagataac tgaggccaaa ctcgatttgg tgttggccaa
     3121 atcttacgtg aacatcagca aaggggccga tcggaaccaa ttgacattca aggcggccga
     3181 tatcgaagcg ggcagcaatg tgttggtgta cggacgcggt tccacacagg gaacacagca
     3241 ggtgatccgc ggattaaaca acagtaatgt tatgggaccc agagctgtct tgcacagctt
     3301 ggacggtttt gtctgtttcg agatcaacca aaccctttta tccaattaag tgcaaaccat
     3361 caacaaggca aggatgaaaa atatactgaa taaatgcctc ttaatcgctt tactggcctg
     3421 cctgccggga ttgatacagg ctcagagagt attgatacgc ggagtggtaa ctgcctacga
     3481 agacaagcag cctttgccgg gagcttatct tttgttgcaa aacagcgacg gtcgtgtggt
     3541 tgccaatacc attaccgatt acgaagggaa ttacagcctt ctgtccgtga taaaacccgg
     3601 cgacgtgctg gtagtttcct attcgggatt caagaaacag accattcccg tggaaaatcg
     3661 cgaaatcatt aacgtcgaaa tgcgggagga aatcctggaa ctggaaaccg ctaccgtagt
     3721 agggcggcga cagatcagtt cgggaatgat gagcataagc gaacgtgatc tgactacggc
     3781 tgccgtcagg atagaaatga gtgaactaga agacctggcc gtggccagta tcgacgacgc
     3841 tttgcagggc agagtggccg gtatggatat ggtgaccact tcgggagctc ccggtgccgg
     3901 tatgtctatt cgtattcgcg gaaccacctc aatcaacggg agttctcaac cctacattat
     3961 tgtggatggt ttcccttacg aaaccactat ctcggaagat tttgactttt caacagccga
     4021 cgaagaggaa tactctcaaa tgctgaatat tgcgccggga gacatcaaag aaataattgt
     4081 gctcaaagac gctgcagcca ctgctatctg gggctcgaaa gccgctaacg gtgtgttgca
     4141 gattaccacc aaaaggggag gtatcagccc gccgacggtg actctgggct caaaaggaac
     4201 tatgtcgaaa caagctaaag gcattcccac gctgaacggt gaccagtatg tcgggctcat
     4261 cctggaggct atgatgaatt cgggcagaac ctttaatccg gaatcttatc ccgaattggc
     4321 caacgatgcc aacaatccct atttctacca caactacggt caaaacaccg actggttcaa
     4381 tgaactgact cagaccggct ggactcagga ccataacttg gccatcagcg gaggaagtgg
     4441 taaagctcag taccgggcct cggtgggtta ttacaacgaa gagggaacta ccgtaggcac
     4501 cggttttgag cgtattacta cccgcctgaa tgtggattac aacgtctcgg aaaaattcag
     4561 gttcgcggcc aatatgtctt acaattactc agaaaccgat cgcgccttta tttcttacct
     4621 gagaagttcc tacgatgtgt tgagtcaggc ttatgtgcgt gctcccaata tggctgttta
     4681 cgagtacaac gaggcaggca ttctgacagg caatttcttt acgccggctt cgaccctgca
     4741 gggttactgg tcgggttcgg ccagcagtat ttacaatccc gtcgccatgg ccgacgaaag
     4801 tatgtacaaa atggtaagca accgtatcat tcctaactta tcggtgatat ggaatccgtc
     4861 ttcggtattg cgctaccagt tcgatgtggg tttcgatgtt atgaacaaca aagccaacgc
     4921 tttcctgccg gtgacagcca cggggcgtcc atggacggaa tctaccgtta acagggccag
     4981 cgacagcgac agcgaggctt ttgttttgca aaccttcaac aaatttttgt acacacccaa
     5041 actgggcgac gatcacagct tacagcttct ggccggagtc aatacctacg ataaaatgga
     5101 gaaaggcttt gccgccaatg cttccaattc ggccagcccc tatctgacgg acccctccaa
     5161 cggtgccaga gtggccggtt tgagtggatt gggtatcagt tcatcgtcgg gacagaaccg
     5221 ttcgattaac ctttttgccc agctacacta taccttgctg gatcgataca ttttcagcgc
     5281 gaccctccgt cgcgacggca gttcccgctt cggacaaaat caccgttggg gtacatatcc
     5341 cagtgcttcg ttgcgttacc gtatatcggg agaaccgttt atgcagaaac tgagtgaaca
     5401 gtggctggat gaactcagcc ttcgtgccag ttacggtgtg aacggtgcac agcccaaagg
     5461 taattatgct tcggtagcca cctacaatgt gtttgatttt gaatacctgg gaggtaccgg
     5521 cacttatcag cgaaatctgg aactggtcga tttgcgttgg gagaacacaa cccaggccaa
     5581 catcggtctg
//
LOCUS       DAOSGL010000129         5580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12880_length_5580_cov_4.051765, whole genome shotgun sequence.
ACCESSION   DAOSGL010000129 DAOSGL010000000
VERSION     DAOSGL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12880_length_5580_cov_4.051765"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="PKY68_05090"
     CDS             complement(<1..1313)
                     /locus_tag="PKY68_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24447.1"
                     /translation="MKKTITRLAFVLLLFTLAVAKTAAIITDPEDYVWYDTGKLGVAD
                     PSTTFPDFFEFGKCFFGISNHDDVDVTPNKLTTRGEALLNACGFYDKTDQTSKVYGIT
                     MPSSSSTDVTVLTLKLPFCDSIYVEGWRTSGSRGIRVQSNLSDDYGFMGKSSGYLITT
                     GLRPRSKDSVTIYIYNTTKDSPTSYGGSLFINRIKIYKDIEEGDVPVFDGVVAGWDFL
                     YRPKASGTVDLESPVNVGEGEWESQHYPLYASDLGVVRGKLSSVSPAGGMQLGNDCGV
                     QAIKFASAGCNDTVDFSLGTEHNNYFQVEMSTLGYQELSLDFDFNIRDASDSCVVAYS
                     TTGNTFTVAGKFPTPDYDGITHVSLNLPELTNQPKVFIRLLLDNGTYGPGGEFILANL
                     SVNGFEYEEYKPEALDIAYINTAASRIRIQERATTSDSLDLVILPA"
     gene            complement(1351..1725)
                     /locus_tag="PKY68_05095"
     CDS             complement(1351..1725)
                     /locus_tag="PKY68_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOH24448.1"
                     /translation="MSDSKNTGTSDSKNIILSDSKIFYSIGEVARMFDLNESTLRFWE
                     KEFDSIKPKKTTKGFRQYTKADIEDIRLVYHLVKERGMTLVGAKKVIRNNKRKLSAKV
                     ELLDRLNAIKEELVAIRDEITE"
     gene            complement(1743..2720)
                     /locus_tag="PKY68_05100"
     CDS             complement(1743..2720)
                     /locus_tag="PKY68_05100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH24449.1"
                     /translation="MAKKSIKYRFNEDTLSFEPIESKAKDKLKTFTLHSILGLVFGFI
                     AFIIYSNFFDSPEAKRLKDQNMELEAQYEILLRQTQEIGSMLTDLEQRDENMYRAILQ
                     AEPIPSELRRGNFERTNRYGSLAQMNHNALVMMTTREVDRLSRRVYIQSKSYDELVEL
                     MRQNEEKLLCIPSIQPVLNEDLKREASGYGWRIDPIYGTRKFHKGMDFSAPVGSDVYA
                     TGNGVVVYAGYERGGYGNFIRINHGFNYETYYAHLSKILVKRGQKVVRGEVIAKVGNT
                     GKSVGPHLHYEVRLRGQPQNPVHYYFMDLSPEEYDDMLQILANTGKAMD"
     gene            2805..5423
                     /gene="alaS"
                     /locus_tag="PKY68_05105"
     CDS             2805..5423
                     /gene="alaS"
                     /locus_tag="PKY68_05105"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOH24450.1"
                     /translation="MLGAKEIRESFKNFFASKGHQIVPSAPMVIKDDPSLMFTNAGMN
                     QFKNIFLGQAPAKYLRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYF
                     KKEAIAWAWEYLTEVLKLDASRLYATVFEGSPEEGLQRDDEAAGYWANYLPKEHIIDG
                     NKHDNFWEMGETGPCGPCSEIHIDLRSEQERAQLDGLHLVNKNHPRVVEIWNLVFMQF
                     NRKADGSLEVLPAKHIDTGMGFERLCMAVQGKTSNYDTDLFQPLIKEIGNLCDVPYGR
                     DQQTDVAMRVVADHIRTIAFSITDGQLPSNTGAGYVIRRILRRAVRYAYTFLGRRRAF
                     LYKLIPALTEVMGSAYPELKAQQNLVEKVIKEEEDAFIRTLESGIKMLEKSMEHLKAS
                     GSTKMDGKEVFSLYDTFGFPLDLTELILRENQLEADIEGFEAEMQQQKARARNAAAVK
                     AGDWVILKDIGYVFVGYDCIECEVEIVRYRQVQQKGKTFYQLVLDKTPFYAEMGGQVG
                     DSGYLEAEGERLPIIEVKKENDLSVHICTKLPADPTRVFRAVVDRERRNRISANHTAT
                     HLLHEALREVLGTHVEQKGSYVSDELLRFDFSHFQKMSEDEIRRVERLVNRKIRSCIE
                     LDEHRHTSMQEAREMGAIALFGEKYGEEVRVVRFGSSIELCGGTHASSTGRIGYFCIL
                     SESSIAAGVRRIEALTGEKAEEHIDALKDKLSQIREMFNQAPGYIDSIRKTIDENHQL
                     RKELEALMAEKVQQIKAEIKHQAVDTNGFLVIRLTMEISPDQVKNIAFQLRGECADNV
                     LFVAGTSFDNKPTLSIMLGKDLVDKGLNAGKLIREAAQFIQGNGGGQAHFAMAGGKDP
                     SGLQQAVQFVVDIVTA"
BASE COUNT         1439 a         1304 c         1388 g         1449 t
ORIGIN      
        1 gcgggcagaa tcaccaaatc cagagagtcg gaggtggtgg ccctttcctg gatacggatg
       61 cgtgaagccg ccgtattgat ataggcgatg tccaacgctt cgggtttgta ttcttcgtat
      121 tcgaagccat tgacgctcag gttggcaagg atgaactccc cgccggggcc gtaagtaccg
      181 ttatccagca gcagacgaat aaacaccttg ggctggttgg tgagctcagg caggttcagg
      241 ctcacgtggg tgatgccgtc gtaatcgggc gtggggaatt taccggccac tgtaaaagtg
      301 ttgcctgtgg tcgaataagc caccacgcaa ctgtctgaag catcgcggat gttgaagtca
      361 aagtccaggg aaagttcctg atagcccagg gtggacattt ctacctggaa gtagttatta
      421 tgttccgttc ccaggctaaa atcgaccgta tcgttacagc cggccgaggc aaacttaatg
      481 gcctgcactc cgcaatcgtt gcccagttgc atacccccgg cgggcgaaac actcgatagt
      541 ttgccgcgca ccacacccag atcggaagcg tacaaggggt aatgttgcga ttcccattcg
      601 ccctcaccga cgttaaccgg cgattccaga tcgaccgtac cactggcttt gggacggtac
      661 aaaaaatccc agccggcaac aactccgtca aagacgggaa cgtcgccctc ttcgatgtct
      721 ttgtatattt tgatgcgatt aataaataga gagcctccgt atgaggtggg agaatctttg
      781 gtcgtattat atatgtatat ggtaacggag tccttggagc gggggcgaag tcccgtcgtt
      841 atcaggtagc cgctggattt tcccatgaaa ccataatcgt cgctcaggtt gctttgtacg
      901 cgaatacccc tgctgccgct ggtacgccag ccttccacat agatggagtc gcaaaaaggt
      961 aattttagtg tcaacactgt tacatcagtc gaagagctcg aaggcatcgt aataccgtaa
     1021 accttgctgg tttggtcagt tttgtcgtaa aaaccgcaag cattcaacaa ggcttcgcct
     1081 ctggttgtga gcttgtttgg agttacgtct acatcgtcgt gattggaaat tccaaaaaag
     1141 cattttccaa attcgaagaa gtcggggaag gttgtcgagg gatcggcgac acccaatttt
     1201 ccggtatcgt accacacata atcttcggga tcggtaatga tggcagcagt tttagctacc
     1261 gccagcgtaa agagaagaag cacaaatgct aatcgggtaa tagtcttttt cataaacgtt
     1321 caggtattaa aaagttggtg tataaaaatc ttattctgtt atttcatctc tgatggccac
     1381 caattcttct ttaatggcat tcagtctgtc gagcagttcc actttggctg agagtttgcg
     1441 cttgttgttg cggatcactt ttttggctcc caccagggtc atgcctcttt cttttaccaa
     1501 atgatagaca aggcggatgt cttcgatgtc ggctttggtg tattgacgga agcctttggt
     1561 ggtttttttg ggttttatgc tgtcgaattc cttttcccaa aaacgcaaag tggattcatt
     1621 caagtcaaac atcctggcca cttcgccgat ggaataaaag attttggaat cggacaggat
     1681 gatgtttttc gaatcggatg tgccggtgtt ttttgaatcg gacataactg ttcggtataa
     1741 gattagtcca tggccttacc cgtattggcc aggatttgca acatatcgtc gtattcttcg
     1801 ggcgaaaggt ccataaagta ataatgcacc gggttctggg gctgcccgcg caaacgcact
     1861 tcgtaatgca ggtgcggtcc aacggatttg ccggtattgc ccactttggc aatgacttct
     1921 ccccgcacca ctttttgtcc tctttttact aaaattttac tgaggtgagc ataataggtt
     1981 tcgtagttga aaccgtgatt aattcggata aaattgccgt agcctccccg ttcataaccg
     2041 gcataaacga caacgccgtt cccggttgcg taaacgtccg atcctacagg ggcggaaaaa
     2101 tccattcctt tatgaaattt gcgggttccg taaatcgggt ctatgcgcca gccgtaaccg
     2161 gaggcttccc ttttgaggtc ttcgttcaac accggttgta tggagggaat gcacaagagt
     2221 ttttcttcgt tttggcgcat caactcaacc aactcatcgt acgattttga ctgaatatac
     2281 acccgcctcg aaagccgatc aacctcgcgg gtggtcatca ttaccaaagc attgtgattc
     2341 atttgggcca gactgccgta gcggttggtt ctctcaaagt taccccgtct caattcagaa
     2401 ggaatgggtt cagcttgcaa aatagcccgg tacatatttt cgtcccgctg ttccagatcg
     2461 gtcagcatgc ttccgatttc ctgagtttgc ctgagtaaaa tctcgtactg ggcttccagt
     2521 tccatgtttt gatccttaag ccgcttagct tcgggcgaat cgaaaaaatt cgaataaata
     2581 ataaaagcaa taaatccaaa gaccagaccc agaatgctgt gcagcgtaaa agttttgagt
     2641 ttatctttgg ctttggattc aatgggctcg aaactcagcg tatcttcgtt gaatctgtat
     2701 ttgatgcttt tttttgccac gaatttcttg cttttaattt aaaatcagag gcaaaagtaa
     2761 gaataaaacc gtacttttgc actcgttttt atattaaatt aattatgctt ggcgcaaaag
     2821 agattcgtga atctttcaaa aacttctttg cctccaaggg gcatcaaata gtaccttccg
     2881 cacctatggt gatcaaagac gatcccagcc tgatgtttac caatgccgga atgaaccagt
     2941 ttaaaaacat ttttctggga caggctcccg ccaaatatct ccgggtggcc aattcgcaga
     3001 aatgtctgag ggtgagcggt aaacacaacg acctggaaga agtggggcac gacacctatc
     3061 atcataccat gttcgaaatg ttgggcaact ggtctttcgg cgattatttc aaaaaagaag
     3121 ccatagcctg ggcctgggaa tacttgaccg aagtacttaa actcgatgct tcgaggcttt
     3181 acgcaaccgt atttgaaggc agtcccgaag agggtttgca aagagacgac gaagccgccg
     3241 gctattgggc taattatttg cccaaggaac acatcatcga cggcaataag cacgataatt
     3301 tctgggagat gggcgaaacc ggcccctgcg gaccttgttc cgaaattcat atagatttac
     3361 gttccgagca ggagcgggct caattggacg gattgcacct ggtcaacaaa aatcaccccc
     3421 gggtggtgga aatctggaac ctggtgttta tgcaattcaa ccgcaaggca gacggttccc
     3481 tcgaggtgtt gccggccaaa cacatcgata ccggtatggg cttcgaacgc ctgtgtatgg
     3541 cagttcaggg caaaacctcc aattacgata ccgatttgtt tcagcctttg atcaaagaaa
     3601 tcggcaatct ctgcgatgta ccatacggcc gtgaccaaca aaccgacgtt gccatgcggg
     3661 tggtggccga ccatatacgc accatcgcct tttccatcac cgacggacag ttgccttcca
     3721 acaccggtgc cggctacgtt attcgccgaa ttctgcgccg tgcggtgcgt tacgcctata
     3781 ctttcctggg cagacgccgt gcctttttgt ataaactgat tcccgccctt accgaagtaa
     3841 tgggttctgc ttatcccgaa ctgaaagctc agcaaaacct ggtggaaaaa gtgattaagg
     3901 aagaagagga cgcctttatt cgcactctgg aatcgggcat caaaatgctc gaaaagagta
     3961 tggaacacct caaagcctcc ggttcgacaa aaatggatgg caaagaggtg ttcagccttt
     4021 acgacacctt tggattccct ctggatctga ccgaactgat acttcgtgaa aatcaactcg
     4081 aagccgacat cgaaggtttt gaggccgaaa tgcagcaaca aaaggccaga gcccgcaatg
     4141 ccgccgccgt aaaagccggc gactgggtga tactgaaaga catcggttat gtttttgtgg
     4201 gctacgattg tatcgaatgc gaggtcgaaa ttgttcgtta ccgtcaggtg cagcaaaaag
     4261 gcaaaacatt ttatcaactg gttttggaca aaacgccttt ttatgccgaa atgggcggcc
     4321 aggtgggcga ttccggttat ctcgaggccg agggtgaacg cctgcctatc atcgaagtaa
     4381 aaaaggaaaa cgacctaagc gtgcatattt gtaccaagct tcccgccgat ccgacccggg
     4441 ttttccgtgc agtggtcgac cgggaaaggc gtaaccgtat ttcggccaat catacggcga
     4501 ctcatctttt gcacgaagcc ctgagagaag tgttgggtac tcacgtagag caaaaaggtt
     4561 cttatgtttc ggacgaactg ctgcgtttcg atttttcgca ttttcaaaaa atgagcgaag
     4621 acgaaatccg ccgggtggag cgtttggtca accgtaagat tcgttcctgc attgagctgg
     4681 acgaacatcg ccatacctct atgcaggaag cccgcgaaat gggagccatt gctctgtttg
     4741 gcgaaaaata cggcgaagaa gtccgggtgg tacgctttgg cagttccatc gaattgtgtg
     4801 ggggaacgca tgcttcttct accggcagga taggttattt ttgcatactc agcgaaagct
     4861 caattgccgc cggtgtcaga agaattgaag ctttgacggg cgagaaagcc gaagagcata
     4921 tcgatgcgct caaagacaaa ctctcacaaa tcagagaaat gtttaatcag gctcccggtt
     4981 acatcgactc cattcgcaaa accatagacg aaaaccatca actgcgcaaa gaactggagg
     5041 ctctgatggc cgaaaaggtg cagcaaatca aagccgaaat caaacatcaa gccgtcgata
     5101 caaacggttt cttagtgatt cgtctgacga tggaaatttc gcccgatcag gttaaaaata
     5161 ttgcttttca gttgagggga gaatgcgccg ataacgttct ttttgtagcg ggcaccagct
     5221 tcgacaacaa acccactctg agcatcatgc tgggcaaaga cttggtagac aagggtctga
     5281 atgcaggcaa actgattcgt gaagccgctc aattcattca aggcaacggc ggaggacaag
     5341 ctcatttcgc catggccgga ggcaaggatc caagcggttt gcagcaggcg gtgcagtttg
     5401 tggtggatat agtgaccgct tgagtataaa gccggttttc ggttggtggc tttccgccat
     5461 gaaatccgag cccgctcagc ctctcgatta tggatacaga cgtcttgttg atttttggta
     5521 ttacaatggt attagcactg attataaagt ctattcaatt gctcattgaa cgtatggatg
//
LOCUS       DAOSGL010000130         5579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12883_length_5579_cov_4.069696, whole genome shotgun sequence.
ACCESSION   DAOSGL010000130 DAOSGL010000000
VERSION     DAOSGL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12883_length_5579_cov_4.069696"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PKY68_05110"
     CDS             <1..400
                     /locus_tag="PKY68_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH24451.1"
                     /translation="RNSLAAPELIKPGTYSLAVGNQFQCQLPAYSMQVFRVKEKGSAL
                     NKLHQDKSSLLKIIPNPVYDQKVDIVFEKPDRQSYSLKLYDSNGKLIFQKDRVKDRLT
                     SIDTGRLPASTYLVQVVHNQTKYTGQLILN"
     gene            complement(487..2967)
                     /locus_tag="PKY68_05115"
     CDS             complement(487..2967)
                     /locus_tag="PKY68_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOH24452.1"
                     /translation="MNKNFLLGSIVLLFSACCGQSIPVSSPEKNPMRLFYKQPAAIWE
                     EALPIGNGRLGAMVFGDVETEHLQLNEENVWSGGPGNNIQPELKALLPKIRRLIFEEK
                     YQEAQNLALKALPRDPGTKGNHGMCYQPLGRLFLHQQGEGQVEDYRRELSIDKALLNI
                     KYRRGGIEFRREIFSSFSDDLIVMEISADRPGALDMEIALNGEHPGHRIETAQSALYL
                     RATSSSRENKEGAVDFTVIVKPRLEGGTLELKEQSLQVRKADRLVLFISMASNVKDYN
                     DIGGDPDLLAQTLMDSAFAKSYEQLRNDHIAVYRPLFERVDLNLGSTDAVNLPTNQRL
                     EQFKEGKDPQLVALYFQFGRYLLISSSMPGTQPATLQGLWTDKLSPAWDSKYTVNINT
                     EMNYWPAEVCNLPEMHEPLFAMLRDLSITGAEAADQMYGARGWCIHHNTDRWRISGAV
                     DGAFYGLWPMGGAWLSQHIWQHYLYSGDRAFLEEYYPVLKGASLFLKDVLQEEPLHGW
                     MVLSPSMSPENSHRSGVSIAAGTTMDNQIIYDVFSNLIDAAQVLNIDLEYADSLRLLR
                     EELAPMQVGRWGQLQEWMHDWDRPNDRHRHVSHLYGLFPSNQISPYRNPELFAAARTS
                     LQARGDVSTGWSMGWKVNLWARLLDGDRAMKLITEQLTPAIRPDGSQTGGTYPNLLDA
                     HPPFQIDGNFGCSSGIAEMLLQSQDGALHLLPALPSAWQEGSVKGLRARGGFQVDLSW
                     KQGLADKILIKSTLGGNCRIRSYVPLKGKGLREAEGPNSNPFYQVPEIKAPLMNNEEV
                     LELPKLRSFYEYDVSIPEGESLFLQACR"
     gene            complement(3063..4766)
                     /locus_tag="PKY68_05120"
     CDS             complement(3063..4766)
                     /locus_tag="PKY68_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24453.1"
                     /translation="MKVLLKSCATLVSRKMVFPLFLFLLPLALNAASGQWNSPLAGEP
                     IPYIVTEPESPQKDYKGKSMTVVYLENLGFGKIGQYSNADNVSWLLAQGYRVIELDYA
                     NHSSMAVSLNINRDIIAINNALESGSFCGLNDCSNYRSYILFEGYRIARDVAYFKDDP
                     TVYNYPDGYTEGDSLYMDIIYPANPREPVPVVLSFSYSNSYATYDKNTGELTDANKHM
                     RLFLPYTLGAFDDSFLEGAPARGIAWAMADHPKYCPWGSGKPVGGANDTYKSYQTNPD
                     AAQKVKSAVRTLRAKSQELGLSGKIGIYGFSRGSDAGAMAVGDRSVPEFENAGLHIGL
                     SDDVQAAALGPGVFDFTQIYNTTDDGDRNLETRCPWAWGPLKDNYELWEKMGAAYLVQ
                     SGLSAPVLFFYNTTDSHYYLDQIQKFKEKLEEWAIPPVSLVDYGSGHSVPKDSASLKK
                     LYDFFDQYLTPPSLDEDTTQSSLSAVKNKQLPLMLSYNSSREELSVRFSLTQAAQVEI
                     QLFDMAGMLVFSTEKKAREAGLCTELINLNKLGLQQGIYHVRLRAGGLNTVGKFVFCK
                     S"
     gene            4831..4986
                     /locus_tag="PKY68_05125"
     CDS             4831..4986
                     /locus_tag="PKY68_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24454.1"
                     /translation="MAQKFLKINALSSFFQKKSACFPAHTKIGAPALVQDVREITIFV
                     ALTASIP"
     gene            5067..>5579
                     /locus_tag="PKY68_05130"
     CDS             5067..>5579
                     /locus_tag="PKY68_05130"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOH24455.1"
                     /translation="MKPAIFPLSLLFSLLMLFSLNPAQLQAKELTKAKNTTVPLYRLF
                     EASISNNKAYQNRFTDVELLVSYQAPSGRRLEFRGFFDGDGKGGGDAQNGKVWKMRFM
                     PDETGIWQYEYRWTDDSPGGKGSFNCTAEGAGKGVLQAYKENPHWFAYNGSDPVYLKS
                     YYETGHGSIGQ"
BASE COUNT         1499 a         1411 c         1287 g         1382 t
ORIGIN      
        1 acgcaacagc ctggcagcac ccgaactgat taaaccaggc acatacagcc tggcggtcgg
       61 caatcaattt caatgccaac tgccggccta ttccatgcag gtattcagag taaaggagaa
      121 gggatctgct ctcaacaaac tacatcagga taaaagttcg ctgctgaaaa taattcccaa
      181 cccggtgtac gaccaaaaag tcgacatcgt ttttgagaaa cccgaccgac aaagttacag
      241 cttgaaattg tacgacagca acggcaaact gatctttcaa aaagacagag tcaaggacag
      301 actaacgagc atcgacaccg gccgtttacc ggcgtccact tatttggttc aagttgtaca
      361 caaccaaacc aaatacaccg gccaattgat tctgaactag cccggcgccg gtcttattat
      421 gcccgttata tgtccgcggg acagaaggtt tatgttgttg tttgccttac caaagggcac
      481 agcgctttat ctgcaagcct gtaagaacag actttcgccc tcgggaatcg agacatcgta
      541 ttcgtaaaaa gaccttagtt tcggaagctc aagaacctcc tcgttgttca tcagaggagc
      601 cttgatctca ggaacctgat aaaacggatt actgttagga ccttcggcct ctctcaagcc
      661 tttgcccttg agcggcacgt aggaacgaat acgacaatta ccacctaatg ttgacttgat
      721 caaaatctta tcggccagac cttgtttcca ggataaatcc acctgaaaac cgcctctggc
      781 tctgaggcct tttacactgc cttcctgcca ggccgaaggc aaagccggca acagatgcaa
      841 ggctccatcc tggctttgca gcagcatttc ggctatcccc gaactgcagc cgaaattgcc
      901 gtcaatttgg aaaggcggat gagcgtccaa taaattggga taggttcctc ctgtctggct
      961 gccgtcaggc ctgatggcgg gagtgagctg ttcggtgata agcttcattg cccggtcgcc
     1021 gtcgagcaga cgagcccaaa gattcacttt ccaacccata gaccaacccg tggaaacatc
     1081 gcctctggcc tgaagcgaag ttctggctgc cgcaaacaat tcgggattgc ggtaaggcga
     1141 aatctggttt gagggaaaca aaccatacaa atgcgacaca tgacgatggc gatcgttggg
     1201 tctgtcccag tcgtgcatcc attcctgcag ctgaccccaa cgcccgacct gcatcggagc
     1261 cagttcttcc ctgagcaacc tcagactgtc ggcatactca agatctatgt tcaaaacctg
     1321 agcagcgtca atcaggttgg aaaacacgtc gtagattatc tgattatcca tggtggttcc
     1381 tgccgctata cttacacccg aacgatgcga attctcgggc gacatggagg gacttagcac
     1441 catccagccg tgtagaggct cttcctgcaa tacgtctttc aaaaacaggg aagctccctt
     1501 cagtaccgga taatactctt cgagaaaagc cctgtcaccc gaatagaggt aatgctgcca
     1561 aatatgctgg ctcagccagg caccgcccat aggccagaga ccgtaaaagg ctccgtccac
     1621 cgctccgctt atacgccagc ggtcggtatt gtggtgtatg caccaacctc tggctccgta
     1681 catctgatcg gcagcctcag ctccggtaat ggaaagatcg cggagcatag caaaaagcgg
     1741 ttcgtgcatt tcgggcaaat tacaaacctc agccggccaa taattcattt cggtattgat
     1801 gtttaccgtg tatttgctgt cccaggccgg actgagttta tcggtccaca aaccctgtaa
     1861 ggtggcaggc tgggtgccgg gcatagagct gctgattaac agatagcggc caaactgaaa
     1921 atacagggca accaactgag gatccttacc ttctttaaac tgctccaaac gttggtttgt
     1981 aggcaaattt acagcatcgg tactgcccag gttcaaatcg accctctcga acagtgggcg
     2041 ataaacggct atatggtcgt tacgcaattg ttcataggat ttggcgaagg ctgaatccat
     2101 cagggtttgc gccaacaggt cggggtcgcc gccgatgtcg ttgtaatctt tcacattgct
     2161 ggccatggaa ataaacaata ccaggcggtc ggctttacgc acctgcaaag attgttcttt
     2221 gagttccagt gtgcctccct caagcctcgg cttaacgata acagtgaagt ccactgcacc
     2281 ttctttgttt tctctgctgc tgcttgtagc cctcagatag agggcagact gagccgtttc
     2341 gatcctgtgt cccggatgtt cgccgttcag tgctatctcc atatccaagg ctccgggacg
     2401 atcggccgat atttccatta cgatgagatc gtcgctgaaa gaactaaaga tttcacgacg
     2461 aaactcgatg ccgcctcgcc ggtattttat attaagcaat gccttgtcta tactcaattc
     2521 acgacgataa tcttccacct gtccttctcc ctgttgatgc agaaacaggc gacccagcgg
     2581 ttggtaacac atgccgtgat tgcccttagt cccgggatct ctgggtaaag cttttaaagc
     2641 gaggttttgt gcttcttgat atttttcctc aaaaatcaat cgacgtattt tgggcaacaa
     2701 agccttgagc tcaggctgaa tgttattgcc ggggcctcct gaccatacat tttcttcgtt
     2761 caattgcaaa tgctctgttt caacatcgcc aaagaccata gcacccagac ggccgttccc
     2821 gatgggcaag gcttcttccc agatggctgc gggctgtttg taaaacaaac gcataggatt
     2881 cttttccgga gaggatacgg gaatactttg cccgcagcag gcgctaaata gtaaaacaat
     2941 gctgcctaat agaaagtttt tattcatgat gtgtagttat taagaaaatg aaacatacaa
     3001 gagataaaac gaccggcata ttactgatgt ttgacgacag gagcgggcga atttttgtcc
     3061 ttttagcttt tgcaaaaaac aaattttcca acagtattca ggcctccggc tctcagtctg
     3121 acatgataaa tgccttgctg caaacccagt ttatttaaat tgattaattc ggtgcataaa
     3181 cccgcttctc tcgccttttt ttctgttgaa aaaaccaaca ttccggccat atcaaaaagc
     3241 tgtatttcaa cttgggcggc ctgcgtcaga ctaaacctta ccgacaattc ctccctggaa
     3301 gaattgtagc ttaacatcaa gggcagttgc ttgtttttga ccgccgataa gctgctttgc
     3361 gtggtatctt catccaagga tggaggggtt aagtattgat caaagaagtc atagagcttc
     3421 ttgagcgaag cggagtcttt gggtacggaa tggccgctgc cgtaatcgac cagactaaca
     3481 ggcggaatgg cccattcttc gagtttttct ttgaattttt gaatttgatc caggtaataa
     3541 tggctgtcgg tggtattgta gaaaaacagc acaggggccg atagtcccga ttgtaccaaa
     3601 taagcggctc ccattttttc ccagagttca tagttgtctt tcaagggccc ccaagcccag
     3661 ggacagcgcg tttctaaatt tctgtcgcca tcgtccgttg tattgtaaat ttgggtaaaa
     3721 tcaaacacgc ccggacccag agcggctgcc tgtacatcgt cggataaacc gatatgtaat
     3781 ccggcgtttt cgaattcagg cacggagcga tcgcccaccg ccatagcacc tgcatcggaa
     3841 ccacgcgaaa aaccataaat acctatttta cccgacaatc cgagctcctg acttttggct
     3901 ctgagcgtgc ggacggccga tttaactttt tgtgcggcgt ccggattggt ctggtaggat
     3961 ttgtaggtgt cgttggctcc gccgacgggt ttaccggaac cccaggggca atatttggga
     4021 tgatctgcca tggcccatgc tatgcctctg gccggtgctc cttccaaaaa ggaatcatca
     4081 aaagctccca aagtataagg caaaaacaaa cgcatatgct tgttggcatc cgtgagttcg
     4141 ccggtatttt tgtcgtaagt ggcgtaactg ttgctgtagg aaaaagacaa aaccaccggt
     4201 actggctccc ggggattggc cggataaata atgtccatat acagggagtc gccttcggta
     4261 tatccgtccg ggtaattgta gaccgtgggg tcgtccttga aataagccac gtcgcgggct
     4321 atgcgatagc cctcgaacaa aatatagctt ctgtagttag aacagtcgtt gagcccgcaa
     4381 aaagagcccg attccaatgc gttattaata gcaataatat ctctgtttat attgagagaa
     4441 acagccatac tactgtgatt ggcgtaatcc agctcaatga cgcggtaacc ctgcgccagc
     4501 aaccaggaaa cattatcggc attgctgtac tggcctatct tgccaaatcc gagattttcg
     4561 agatatacca cagtcatcga cttgccttta taatcctttt ggggcgactc aggttctgta
     4621 acaatatagg gaatcggttc tccggcaagg gggctgttcc actggccgga agcagcgttc
     4681 aacgctaaag gaagcaaaaa caaaaagagc ggaaaaacca tttttctgct caccaaagtg
     4741 gcacaagact ttaataagac tttcatagct aagggttata cgataaaact taagtgccga
     4801 taggcacagt ggattaaaat aagcgtaaat atggcacaaa aatttttaaa aatcaatgct
     4861 ctgagcagtt tctttcagaa aaaatccgcc tgttttcctg ctcatacaaa aatcggagct
     4921 ccggcgcttg tacaagacgt aagagaaatc actatatttg tcgcactcac ggcaagcata
     4981 ccgtaaacca cgtaaacaac gcaaacaaca caaacaacac aagcatacat ctaaaattat
     5041 aacatcttac aaaaattatt accggaatga aaccagccat tttcccctta agtctccttt
     5101 tttccctgtt gatgcttttc agcctgaatc ccgctcagct gcaagcaaaa gaacttacca
     5161 aagccaaaaa cactacagtg cctctataca ggctttttga agcctcgata agcaacaata
     5221 aagcctacca aaatcggttt acagacgttg aacttttggt cagctaccag gctccttcgg
     5281 gccgccggct tgaatttcgc ggtttttttg acggcgacgg caaaggcggt ggcgatgcgc
     5341 aaaacggcaa ggtatggaaa atgcgtttta tgcccgacga aaccggcatc tggcaatacg
     5401 aataccgctg gaccgacgac agcccgggcg gtaaaggcag cttcaactgc acggccgagg
     5461 gtgccggcaa aggcgtactc caagcctaca aagaaaatcc tcactggttt gcctacaacg
     5521 gcagcgatcc cgtgtattta aaatcctatt acgaaaccgg tcacggctcc ataggccag
//
LOCUS       DAOSGL010000131         5578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_12888_length_5578_cov_3.451023, whole genome shotgun sequence.
ACCESSION   DAOSGL010000131 DAOSGL010000000
VERSION     DAOSGL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12888_length_5578_cov_3.451023"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..3155
                     /locus_tag="PKY68_05135"
     CDS             324..3155
                     /locus_tag="PKY68_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24456.1"
                     /translation="MKAKFLVVSLSMIFVFCGCNLSSVRKNTGSWGDWTDSIPPQPKE
                     EYLKFIDDLKSPLWTKHDWLAAPPALNHIDVKKGITLYKNFPDSKGLLATAYDDLETF
                     LRAGDIPVNRNHFVIETAHRPDLDKESFIIETDSFCCKISSGDTEGIRRGIFYLEDQM
                     LSVKGPFLKIGTFKKTTDIRRRISRCAFGPIKRLPLMRDELMDTVNYYPDNYLNRLAH
                     EGVNGLWLTVEFKDLVSSPYLVDGGKNAEKRLKKLSDVVNSCLRYGIRTYIFCIEPRL
                     HVIDVEKYPVFRGANGSFFCPSSQKAYNYLYETVKTIFTEVPNLGGIINISHGERGTT
                     CLSSVPATSSYERIIDCPRCKNKKPWEILYASLLPMEKGMHDVSPEAELISWLYMPEP
                     QNYIGGDDFSLGSWVYDIPAHTPPNVILQFNFESGVTKEVFDKLLVGGDYWISTPGPS
                     DRFVKIANVAKESGTSVSAKIQTGNSHEMATVPYIPVPTLLYRKFDSMKRLGVTHTML
                     SWYFGSYPGLMNKGAGLLSMNAYADVNDFLESLASIYWKEEDIPKLIEAWTLFGEAFS
                     YYPLTNMFQYYGPMHDGIVWPLYIIPQDKYLSPTWLLGSSSQADVKVWPPSGDRIGEC
                     LGWNLTLDEVVVLCKQMADKWEEGMQHLKGIEKKYQHDNDRILDLVVAKAVQIHLRSS
                     YNILRFYLTREKMFRTAFDKEKLEMLLEMEHIVHEEIKQSEEMISLCSKDSRLGYHSE
                     AEGYKYYPEKLKWRVEQLKTVLKNDFPLVRQKITNNEELFPEYTGAKPGGLSMHSFPS
                     SGDIYETAQNIKNWLSFDNEEMGNKIVWGSIYDETNLYIIISDEIGITEGNIQIEIEP
                     RRLWPVKYFIYPIGKSKAGYQTKKIGNKTLSIISIPFADIGDEIGKNTPVRMNLRYGD
                     NVWIPKNPLPARLLLGNANPTDLGWILFK"
     gene            3234..5141
                     /locus_tag="PKY68_05140"
     CDS             3234..5141
                     /locus_tag="PKY68_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOH24457.1"
                     /translation="MKTVPDVGETFDVEAFTDNIRNCGVDYLVFHARCNQGLAYYNTR
                     IGTRHPSLQYDLFGKLAEACQRKGIMLGAYFNAGISSEEGMQHREWTTLYFDGRSLRE
                     PRFTPHVRTMCYNSGYRDHLIAMVKEVLYNYPVAGLMIDCLINYPCVCPVCVTKMKKE
                     GIDWSDLSAVKRFSELSAVRLAKDIAREAKKIKPGLLISNNYPSYEELLDVNTYFDVV
                     SLPASGEGYEFMPVMAHYVRTLGNYPVLNMTGRFYDWGDFGGLLPEEAIKSELLYGLA
                     NGMRPNIGGHFHPRGDWDKPVLERIMKIYTSIQANEEWYDGAKPIAEIAIVYPKSIHH
                     IREDKDLRAAVRMLSELKQQFDVVTLASDWGKYEVLVFPDNVLFDGETAKRVRQHLNE
                     GKAVISTGFSGLDSEKKGFALELEWGIKYLGENPFDPAYFSVGDNYSEGLPDMPLSLY
                     SNGIDIEVLGDTRVEARLVKPYHNRCWDGEYAFYYNPPDKVTDSPALTINAQVAHFSH
                     RIFSGYHDKASVELRTVFSNVLNHFFPKPLVKAENLQSFARVFVTEQPRRKMVHLLSY
                     LPEMRGNTQMIEEPIELSNVTLSLRQDDKNIQNIYLAPQKERLAYSIEGGYIHIKVPK
                     SKGYSLLVIEE"
     gene            5166..>5578
                     /locus_tag="PKY68_05145"
     CDS             5166..>5578
                     /locus_tag="PKY68_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24458.1"
                     /translation="MKKNIIPLFVLIAIVLISCFQKNGTNNQENDIIIENEQCKLVIG
                     SNAIAKSLIYKPTNEECLSRQSKIPVFSVTQERPFHNEIKLAYPTNKMTFNAKSVKKE
                     GDELIVDFELIFYKARIKLDVSPHYINFTVKDFFI"
BASE COUNT         1720 a         1095 c         1277 g         1486 t
ORIGIN      
        1 aactttctca attaaaaaac tgacctgaag ttagatttcc agagtgggtt taagtcagca
       61 ggttccctgg gaatgtaaca ctacctgcaa agatagctaa ccactgtttc atgccccgtt
      121 ttacgatttt aaccgtacgg gattatttta cacatattta caataatctt tgcgcaaatc
      181 atataagata atttaaaaac aatgtttttt gttccctccc tttttttctt attataaaat
      241 gtagtatgtt agttcgaata gtgaatataa cacaaagtaa ataaagatag aaggtcgtat
      301 cattctgttt tccaggataa aaaatgaagg caaaattttt agttgtatcc ctttcgatga
      361 tttttgtatt ctgtggctgt aacttatcaa gcgtacgaaa aaatacaggc tcatggggag
      421 attggacaga ttccatacct ccccagccta aagaagaata tttgaaattt attgatgacc
      481 ttaaatcgcc tttgtggaca aagcacgatt ggctcgctgc tcctcctgcc ctcaatcata
      541 tagacgtaaa aaaaggaatt accctgtaca aaaactttcc cgattcaaaa ggtttgctgg
      601 ctaccgcgta tgatgatttg gaaacgttct taagagcagg agatattccc gtcaatcgaa
      661 accattttgt tatagaaaca gctcataggc cggatttaga taaagaatct tttattatag
      721 agacggattc gttttgttgc aaaataagct caggggatac cgaaggtatt cgccggggaa
      781 tattttactt ggaagatcag atgttaagcg taaaaggtcc ttttttgaaa atagggactt
      841 ttaaaaaaac aaccgacata cggagaagaa tttcccgctg tgcttttggc cccataaaaa
      901 ggctccctct gatgcgcgat gaactgatgg ataccgttaa ttattatccc gacaattacc
      961 tgaatagatt agcgcatgaa ggggtaaacg ggctttggct gacagtagaa ttcaaggatt
     1021 tggtctcttc cccgtacctg gttgatggag ggaaaaatgc tgaaaaaaga ttaaaaaaat
     1081 tgagcgacgt agtcaactct tgtttacgtt atggcattag gacctatata ttctgtatcg
     1141 agcccaggtt gcacgtgatt gatgttgaga agtatcctgt tttccgcggg gcaaacggct
     1201 catttttttg tcccagcagt caaaaggcgt acaactattt atacgaaacg gtcaaaacga
     1261 tttttactga agttcctaat ttaggaggta taataaatat ttcgcacggg gaaaggggaa
     1321 ccacctgcct aagcagtgtt ccggcgactt catcctatga aaggataata gattgccccc
     1381 gctgtaaaaa taaaaaaccg tgggaaatat tatacgcatc gttgcttccc atggaaaaag
     1441 ggatgcacga tgtttctccc gaagctgaac taattagttg gttatacatg ccagaacctc
     1501 aaaattatat agggggagat gatttttctt taggtagttg ggtttatgat atccccgccc
     1561 atacccctcc aaatgttatc ctccaattca attttgagtc gggtgtaacc aaagaggtat
     1621 ttgataaact tctggttggt ggcgactatt ggatatctac tcccggcccg agtgatcgat
     1681 ttgttaaaat agcaaacgta gcgaaagaat caggaacgtc tgtctccgct aaaatacaaa
     1741 cgggtaattc tcatgaaatg gccacggtac cctatattcc cgtacccaca ttgctatatc
     1801 ggaaattcga ctcgatgaag cgattaggtg ttacccatac catgttgtca tggtacttcg
     1861 gaagctaccc ggggttgatg aataagggag cagggttgct ctccatgaat gcttacgcgg
     1921 atgtaaatga ttttttggaa tcgttggcat caatttattg gaaggaagaa gatatcccta
     1981 agttaattga agcatggaca ttgttcggag aagcattttc ttattaccct ttaacaaaca
     2041 tgtttcaata ctacggacca atgcacgacg gcatagtttg gccgttatat ataattcctc
     2101 aagacaaata cctttccccg acctggttac tgggttcaag ttcccaggca gacgtgaaag
     2161 tgtggcctcc aagcggtgac cggatcggag aatgtttggg atggaatctc acgttggatg
     2221 aagtggttgt tttatgcaaa caaatggcag ataagtggga agaaggcatg caacatttaa
     2281 agggtataga gaagaaatat cagcacgata atgatcgaat actcgatctt gttgtggcaa
     2341 aagctgtaca aatacattta agaagtagtt ataatattct tcgattttat ttaacgcgtg
     2401 aaaaaatgtt tcgtactgct tttgacaaag aaaaattgga aatgctgcta gaaatggagc
     2461 atatcgtgca tgaagagatc aagcagtctg aagagatgat atctttgtgc tccaaggatt
     2521 ccagactcgg atatcactct gaagcagaag gatataaata ttatccggaa aaattaaaat
     2581 ggagagttga acagcttaag actgttttga aaaatgattt ccctttagta aggcaaaaaa
     2641 taacaaacaa cgaggaatta ttccctgaat atacaggggc aaagcctggg gggttatcaa
     2701 tgcacagttt tccgagcagt ggagatattt atgagactgc tcaaaatatc aaaaactggt
     2761 tatcctttga taacgaggaa atgggaaata aaatagtatg gggttccatt tacgacgaaa
     2821 ctaatttata cattatcatt tcagatgaaa ttggaataac ggaaggaaat attcagattg
     2881 aaattgaacc acgaaggcta tggccagtaa aatatttcat ctacccgata ggaaaaagta
     2941 aggcaggcta tcaaacaaaa aaaatcggaa ataaaacgct gagtattatt agtattcctt
     3001 tcgctgatat aggggatgaa atcggaaaaa atactcctgt acgaatgaac ttgcggtatg
     3061 gagacaacgt gtggatacct aaaaatccgt tacccgctcg tttattgtta ggtaacgcca
     3121 acccaacgga cctggggtgg atactattta aataacaaag acatataaca gaatataagg
     3181 cttatcgcat gaaacacaga cctaaatact gtttgttcta tgataatcac acgatgaaga
     3241 ctgttccaga cgtgggagaa acgttcgacg tggaagcatt taccgataac atcaggaact
     3301 gtggagtcga ttacctcgtg tttcatgctc gttgtaatca ggggctcgcg tattacaata
     3361 cccggatcgg aacaaggcac ccttcgttgc agtacgacct tttcggcaaa ctggccgaag
     3421 cctgtcaacg aaaggggatc atgctggggg cgtactttaa cgcgggtatc agcagcgaag
     3481 aaggcatgca acaccgggag tggacaacct tgtatttcga tggccggtcg ttacgcgagc
     3541 cgcgtttcac accgcatgtc cgtaccatgt gttacaactc cggttaccgc gaccacctga
     3601 ttgctatggt aaaagaggta ctttacaact accccgtcgc ggggctgatg atcgactgcc
     3661 tgataaatta cccctgcgtc tgcccggttt gcgtgacaaa gatgaaaaaa gaaggtatag
     3721 actggtcgga tttaagtgct gtcaagcgtt tctcggagct ttcagcagta cgcctggcaa
     3781 aagatatcgc gcgggaggcg aagaaaataa agccggggtt acttatctcc aacaactacc
     3841 cgagttacga agagttactg gacgtgaaca cctacttcga cgtggtttcc ttgccggcgt
     3901 cgggcgaggg atacgagttc atgcccgtga tggctcatta cgtgaggaca ctgggcaatt
     3961 acccggtctt gaacatgacg ggccggtttt acgattgggg cgatttcggc gggttgctgc
     4021 ccgaggaagc cattaagtcg gaattgcttt acgggttggc caacggtatg cggccaaaca
     4081 taggcggaca ttttcacccc aggggcgatt gggacaagcc ggtactggag agaataatga
     4141 aaatatacac cagcatacag gctaacgagg agtggtacga cggcgcgaaa ccgatagctg
     4201 aaatcgccat agtgtaccct aaatccattc atcatatccg ggaggacaag gatttgaggg
     4261 ctgcagtacg tatgttaagc gaattgaaac aacagttcga cgtggtgaca ctggcatcgg
     4321 actggggcaa gtatgaggtt ttggtattcc ccgacaatgt tctgtttgac ggggagacgg
     4381 caaaacgagt gaggcaacac cttaacgagg ggaaagcggt catttcaacg gggttctccg
     4441 gtctggattc cgaaaagaaa ggtttcgcgc tcgaattgga atggggcatc aaatatttag
     4501 gcgagaaccc gtttgacccg gcctacttta gcgttggaga caactacagc gagggtttgc
     4561 cggacatgcc gctctcgttg tactccaacg gcattgacat agaggtgctg ggcgacacga
     4621 gagtagaggc acgcctcgtg aagccgtacc acaaccggtg ttgggacggg gaatacgctt
     4681 tttactacaa tcctcccgat aaggtaactg atagcccggc gcttacaatt aacgcacagg
     4741 tggcccattt cagccaccgg atattttccg gatatcacga taaggcatcc gtggaattaa
     4801 gaacggtttt ttccaacgtg ctcaaccatt ttttccctaa accgttggta aaagcggaaa
     4861 atttacagtc gttcgcacgg gttttcgtca cggaacaacc ccgaaggaaa atggtgcatt
     4921 tactctctta tttgcctgaa atgaggggaa atacgcagat gatcgaagaa cccattgaac
     4981 ttagcaacgt aaccctatcc cttcgccagg atgataaaaa tattcagaac atctacctgg
     5041 caccccaaaa agaaaggctg gcttattcca ttgagggagg ttacatccat atcaaggtgc
     5101 ctaaaagtaa aggatactct ctgttggtta tcgaagaata aacaaacaac gaatactatg
     5161 cagttatgaa aaagaatatt attccattat ttgtgttgat tgccattgtt ttaatctcat
     5221 gttttcaaaa aaatggcact aataaccagg agaatgacat cattattgaa aatgagcaat
     5281 gtaaattggt tatcggaagt aacgccatcg ccaaaagtct gatatataag ccaaccaatg
     5341 aagagtgtct ttcacgtcaa tcaaaaatac ctgttttctc cgtcacccag gaaaggccat
     5401 tccacaatga aataaaactg gcatatccaa caaacaagat gacgttcaat gctaaatccg
     5461 ttaagaaaga gggtgacgaa ctgattgttg attttgaatt gatcttctat aaagcacgta
     5521 taaaattgga tgtaagcccc cactatataa actttacggt aaaagatttt tttataga
//
LOCUS       DAOSGL010000132         5516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13069_length_5516_cov_3.730086, whole genome shotgun sequence.
ACCESSION   DAOSGL010000132 DAOSGL010000000
VERSION     DAOSGL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13069_length_5516_cov_3.730086"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="PKY68_05150"
     CDS             complement(<1..1459)
                     /locus_tag="PKY68_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOH24459.1"
                     /translation="METPVNKPKQLRIALRNCGMLDPENIAESIVRDAYQALGKVLKE
                     YSPQECINILKQSGLRGRGGAGFPTGLKWEIASKSKSDQKYVVCNADEGDPGAFMDRS
                     ILEGDPHSVLEAMAICGYCIGADKGLIYIRAEYPLAIKRLKIAISQAMEWGLLGKNIL
                     GSGFNFEIKLCYGAGAFVCGEETALIHSMEGKRGEPTNKPPFPATSGFMKKPTNVNNV
                     ETFANIAPIILKGPEWFSSIGTKNSKGTKVFALAGKINHVGLIEVPMGTSLREIIYEI
                     GGGIKENKKFKAVQTGGPSGGCLTEKHLDTPIDYENLIAAGSMMGSGGMIVMDENDCM
                     VSVAKFYLDFIVGESCGKCTPCRIGNKRLYEILERITQGKGVMKDLDKLKNLSYIIKD
                     TALCGLGQTSPNPVFSTLENFYDEYYEHVVEKKCRAGHCKNLFHYVINEELCVGCAAC
                     KRCCPVHAITGEKRKPHYIDSNICIRCNECIEKCSF"
     gene            complement(1470..1862)
                     /locus_tag="PKY68_05155"
     CDS             complement(1470..1862)
                     /locus_tag="PKY68_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOH24460.1"
                     /translation="MTHIKSLEQLTSKRDELKSNLSRLDDPNNSEKKVQIKVAMATCG
                     IASGARTTMDFLTAELSKRNIPSVVTQTGCMGYCYAEPTIEVKLPGKEAVIFGYVDTG
                     RADEIIEKYIKTGELTEGVIPRNYDTID"
     gene            complement(2124..2198)
                     /locus_tag="PKY68_05160"
     tRNA            complement(2124..2198)
                     /locus_tag="PKY68_05160"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2161..2163),aa:Pro,seq:tgg)
     gene            complement(2248..3654)
                     /locus_tag="PKY68_05165"
     CDS             complement(2248..3654)
                     /locus_tag="PKY68_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOH24461.1"
                     /translation="MGGFFGTISNSSCIKDLYYGIDYNSHLGTRRGGMATLYNGKFYR
                     AIHNLENAYFRSKFEPELLSFLGNSGIGVISDTDPQPIVINSRLGKFAIVTVACVNNI
                     KELEKDLLDSGRHFSELSSGQTNQTELIALIITQGKTFVEGIELVFQKVKGSCSMLIL
                     TEEGIIVARDKFGRTPIVIGKKDGAYAASSEPCAFPNLDYKTDYCLGAGEIVLLRPNN
                     MQQLREPNKRKQVCSFLWVYFGYPVSDYENINVDSVRYKCGLTMGNNDDSEVDFVSGI
                     PDSGVGLGLGYAEGKGVPYRRAIVKYTPTWPRSFTPSDPGIRKLVAKMKLIPNRSLLT
                     GKRVIFCDDSIVRGTQLSDNVSELYKYGAKEVHIRIGCPPLIYTCPFLHFTATKSDLE
                     LITRRIIQEIEGAHDKNLSEYSKTDSVKYRQLVEHIRARFDITSLKFNSLENLVKAIG
                     LPKESLCTHCFDGSSYFD"
     gene            complement(3673..>5516)
                     /locus_tag="PKY68_05170"
     CDS             complement(3673..>5516)
                     /locus_tag="PKY68_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOH24462.1"
                     /translation="TLVLKQQQTIEELPEDILLGAKRLGFSDFQIARFVENPKGNMEK
                     DNIRVRDRRKQLGVLPRVKRINTVASEHPELTNYLYLTYEGETHDIPYYPNEKSVVVL
                     GSGAYRIGSSVEFDWCSVNAIQTARNLGYKSIMINYNPETVSTDYDMCDRLYFDELSF
                     ERVMDVIDLERPKGVIVSVGGQIPNNLAMKLYRQQVPILGTSPVSIDRAENRHKFSSM
                     LDQLGIDQPAWKELTSLEDIKIFIEKVGYPVLVRPSYVLSGAAMNVCYDEDDLESFLK
                     MAAKVSKEFPVVVSQFLQNAKEIEFDAVAHNGDVVEYAISEHVEFAGVHSGDATMVFP
                     AQKIYFATARRIKKISRCIAKELNISGPFNIQFLAKNNEVKVIECNLRASRSFPFVSK
                     VLKRNFIDTATRIMLDAPYDKADKNAFDIDWIGVKASQFSFSRLHKADPVLGVDMSST
                     GEVGCLGDDFGEALLNAMIATGFNIPDKKKGIMVSSGGSKSKVDLLDACIMLQENGYP
                     IYATGGTQQFLKEHGVKAELVHWPDENNDKRQILNMISDRAFDLLINIPKNLTKRELT
                     NGYKIRRAAIDHNIPLITNARLAAAFIESFCSIDMNDIQIKSWQEYI"
BASE COUNT         1440 a         1217 c         1249 g         1610 t
ORIGIN      
        1 cgaaagagca tttttcgatg cattcattac agcggatgca gatattggaa tcgatgtaat
       61 gaggtttgcg tttttcgccg gtgatggcgt gtaccggaca acacctttta caggcagcac
      121 aacccacgca caattcttcg ttgataacgt agtggaacag atttttacaa tggccggcac
      181 gacatttttt ctcgaccaca tgttcgtagt attcgtcgta aaaattctcc agagtggaaa
      241 acacagggtt gggagaggtt tgtcccaaac cgcaaagagc agtgtcttta attatatagc
      301 tgagattttt cagtttatcc aggtctttca ttactccttt gccctgggtg attctttcca
      361 aaatttcata caagcgttta ttgccgatcc ggcaaggtgt acatttaccg caggattctc
      421 ctactataaa atcgagataa aatttagcta ccgaaaccat acagtcgttt tcgtccataa
      481 cgatcattcc tccggaaccc atcatagaac cggcggcaat cagattttcg tagtctatgg
      541 gcgtatcgag gtgtttctca gtcagacaac ctccagaagg tcctccggtt tgaaccgcct
      601 taaatttttt gttttctttg attcctccgc caatctcata gatgatttct ctcaggcttg
      661 tacccatagg gacttcgata agtccgacat gattgatttt acctgccagt gcaaacactt
      721 tggtgccttt ggaatttttg gtcccgatgg acgaaaacca ttccgggcct ttgagtatga
      781 tgggagctat gttggcaaat gtttccacat tgtttacatt ggtgggtttt ttcataaaac
      841 cgctggtggc aggaaaagga ggtttattgg tgggttctcc gcgcttcccc tccatagaat
      901 gaatcagggc ggtttcttca ccacaaacaa aggctccggc accgtagcag agcttaattt
      961 cgaaattaaa accgctaccc aggatgtttt tgcccagcaa gccccattcc atcgcttgac
     1021 tgatagctat ttttaatcgt ttaatagcca atggatattc tgctcgtatg tagatgagtc
     1081 ctttgtcggc tcctatgcaa taaccgcaaa tggccatagc ttcgagcacc gaatgtggat
     1141 cgccctcgag tatggatcga tccataaagg ctcccgggtc gccttcgtcg gcattgcaaa
     1201 cgacgtattt ttgatcggat ttacttttgg aggcaatttc ccatttgagc ccggtgggga
     1261 aaccggcccc tccgcgtcct cgaaggccgg attgtttcaa aatgtttatg cattcctggg
     1321 ggctgtattc ttttaacact ttacccaggg cctgataggc atcccggaca atagattccg
     1381 ctatgttctc cggatcgagc atgccacagt tacgcagggc aatacgcagc tgcttgggtt
     1441 tattgacagg tgtttccatc gtactatgat taatcgattg tatcgtaatt ccggggaata
     1501 actccctctg ttaattctcc cgttttaata tatttttcaa taatctcatc ggctctgccg
     1561 gtatccacat acccaaagat tacggcttct ttgccaggta atttcacttc aatagtgggt
     1621 tcggcataac aatagcccat gcagccggtt tgagtaacca cagagggaat atttcgctta
     1681 ctgagttcgg cagtcaaaaa atccatagtg gtacgcgcgc ccgaagcaat accgcaggtt
     1741 gccatggcaa ccttgatctg tacttttttc tcggaattgt tggggtcatc cagccgggat
     1801 aagttcgatt tgagttcatc ccttttgctt gttagttgct caagcgactt gatgtgtgtc
     1861 atgaaaatta aggttaatgt agaacgaaaa taaataacag caaaaagctt taataaaaaa
     1921 ggattttgct gtgttttgta aaaacactgt aaagatatac aataatattc tatttacaga
     1981 attttttccc taattaatca ggaattattt tcgttgtttt caatcggaga aaaaataaga
     2041 aacgaccctc tctcattagt tggagtaaaa gggtcgtttt tggggaaaaa tctcatcgca
     2101 ataaaggcgt tgtgattcac ttgtcggggt aacaagattc gaacttgtga ccccctgctc
     2161 ccaaagcagg tgcgctaacc ggactgcgct acaccccgaa tatttccggc gacaaagata
     2221 gtgtttttta tttatttcag aacaaactca atcgaaatag ctggagccgt cgaagcaatg
     2281 agtacataag ctttctttag gtaaaccaat ggctttgacc aagttttcga gtgagttgaa
     2341 ttttagggat gtaatgtcaa accgggcacg gatgtgttcc accagttgtc ggtatttgac
     2401 agaatcggtt ttagagtatt cagacaggtt tttgtcgtga gccccttcga tctcctgtat
     2461 tatccggcgg gtaatcagtt ccaagtcgga cttggtcgcg gtaaaatgca gaaaaggaca
     2521 ggtataaatg agcggcggac agccgatgcg tatatgcact tctttggctc catacttgta
     2581 cagttcgctc acattgtcgg aaagttgtgt gccacgtact atggagtcgt cgcaaaagat
     2641 aacccttttg ccggtgagta gactacggtt gggtatcagc ttcattttag ccacaagttt
     2701 acggataccc gggtcgctgg gagtgaagct tctaggccag gtgggggtgt attttactat
     2761 ggcgcgccgg tagggaacgc ctttaccttc ggcgtagccc aaacctagac ctacgcctga
     2821 gtcgggaata ccggagacga aatctacttc tgaatcgtcg ttgttaccca tcgttaaacc
     2881 acatttataa cggactgaat ccacgtttat attttcatag tcggaaacag gatacccaaa
     2941 ataaacccac aaaaaggaac agacttgttt gcgtttgttg ggctcacgca attgctgcat
     3001 gttattcgga cggagcagaa caatttcacc tgcaccaaga cagtagtccg ttttataatc
     3061 gaggttggga aaagcacagg gttcactcga agcggcataa gctccgtctt ttttgccaat
     3121 gacaatgggg gtgcgtccga acttgtcccg ggcaacgatg attccttcct cggtaaggat
     3181 cagcatggaa caagatcctt ttactttttg aaaaaccaat tcaatgcctt cgacaaaagt
     3241 cttgccttgg gtaatgatca gggcaatcag ttctgtctga ttggtttgcc ctgaactgag
     3301 ttcggaaaaa tgcctgccgg aatcgagtaa atctttttcg agttctttta tgttgttgac
     3361 acaggccact gttactatgg caaattttcc cagacgagaa ttgattacga taggctgagg
     3421 atcggtatca ctaatgacac ctatgcctga gtttcccaaa aaagaaagta gctccggctc
     3481 gaattttgag cgaaaataag cattttccaa attgtgaatg gccctataaa acttgccgtt
     3541 atataaagtg gccatgcctc cgcggcgggt tcccaggtga gagttgtagt ctatgccgta
     3601 ataaaggtct ttgatacaac ttgagttgga gatggttcca aaaaagcctc ccatattgcg
     3661 atgataggta aattatatat attcttgcca gcttttaatc tggatatcat tcatgtcgat
     3721 ggagcagaag gactcaataa aggctgctgc caacctggcg ttggtaatca acggtatatt
     3781 gtgatctatt gcagccctgc gaattttata tccgttagtt aactctcttt tagtcagatt
     3841 tttgggtata ttaataagca aatcaaaagc gcgatcagag atcatgttta atatttgtcg
     3901 cttgtcgtta ttttcgtcgg gccagtgtac caattcggct tttacaccgt gttcttttaa
     3961 aaattgctga gttcctccgg tggcataaat gggatagccg ttttcttgca acataatgca
     4021 ggcatctaat aagtcaactt tcgatttgct ccctccggaa gaaaccataa tgcctttttt
     4081 cttatcagga atgttaaaac ccgtagcaat catggcgttc aacaaggctt cgccgaaatc
     4141 gtcgcccaga cagcctacct cgccggtgga cgacatgtct acgcccagta caggatcggc
     4201 tttatgtaaa cgggagaagg agaattggga agctttaaca cctatccagt cgatatcaaa
     4261 agcattttta tctgctttgt cgtagggggc atccagcatg atgcgggtag ccgtatctat
     4321 gaaattacgt ttaagtactt tagatacaaa ggggaagctc ctcgaagctc tgaggttgca
     4381 ttcaattacc ttgacctcgt tgtttttggc cagaaactga atattaaacg ggccgctgat
     4441 gttgagttct ttagctatgc agcgactgat tttcttgatg cgtctggctg tggcaaaata
     4501 gattttttgt gccgggaaaa ccatcgtggc atcgcccgag tgtacaccgg caaattccac
     4561 gtgttcggag atagcgtatt caaccacatc cccgttgtga gccacagcgt caaattcaat
     4621 ttctttagcg ttttgtaaaa actgtgaaac caccaccgga aattccttag acactttagc
     4681 cgccattttc aggaaacttt ccaaatcatc ttcgtcgtaa cagacgttca tggccgcacc
     4741 cgataacaca tacgaaggcc ttactaacac aggataaccg actttttcaa taaatatctt
     4801 gatgtcctct aagctggtta gctctttcca ggccggttga tctatgccca attgatcaag
     4861 catactcgaa aacttgtgtc tgttttcggc ccggtcgatg gaaaccggag aagtgcccag
     4921 gatcggaact tgctgacggt aaagcttcat ggccaggttg ttgggtattt ggccgccaac
     4981 agaaacgatc acacctttgg gacgttccaa gtcgatgacg tccatcacgc gctcaaaact
     5041 cagttcatca aaatacaaac ggtcgcacat gtcgtaatcg gtcgataccg tttcggggtt
     5101 gtaattgatc atgatggact tgtaacccaa gttgcgggct gtttggatag cattgaccga
     5161 acaccagtcg aattctacgg aactgccgat gcggtaagca ccggaaccaa gcactacaac
     5221 cgatttttcg ttgggataat agggaatatc atgagtttca ccttcgtaag tcagataaag
     5281 gtaattggtc aattcgggat gttccgaagc gactgtgttt atgcgtttta ccctgggcag
     5341 cacaccgagc tgtttgcgac ggtcccgtac gcggatgttg tctttttcca tattaccttt
     5401 aggattttcg acaaaacggg caatctgaaa atccgagaaa ccgaggcgtt tagccccgag
     5461 taatatatcc tcgggcaatt cctcaattgt ttgctgttgt tttaaaacca gagtgt
//
LOCUS       DAOSGL010000133         5513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13077_length_5513_cov_4.158483, whole genome shotgun sequence.
ACCESSION   DAOSGL010000133 DAOSGL010000000
VERSION     DAOSGL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13077_length_5513_cov_4.158483"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="PKY68_05175"
     CDS             complement(<1..131)
                     /locus_tag="PKY68_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOH24463.1"
                     /translation="MTVKIVNRSKHPLPQYATSLSAGMDLRANIDAAVCIKPFERKL"
     gene            310..825
                     /locus_tag="PKY68_05180"
     CDS             310..825
                     /locus_tag="PKY68_05180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOH24464.1"
                     /translation="MKPAYLYLFLLGVLFSITSCLDDPEVTAWRKANDEFMLSLRDSS
                     DFKPLEYNDRILYGDENYNYLDHPVSGIYYKTLVEGRGAVPLVGQTVQVEYEGRLYDG
                     TVFDSGSRTTLVGTGVISGWSEVLYRIPQGSTWEVAIPYHLGYGEVKHGSIPAYSCLI
                     FTMELVSIVED"
     gene            complement(832..1836)
                     /locus_tag="PKY68_05185"
     CDS             complement(832..1836)
                     /locus_tag="PKY68_05185"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOH24465.1"
                     /translation="MRLAIVGASGAVGQEFLKVLDERNFPMDDLVLFGSGRSAGKTFS
                     FRGKSIVVKELKHNDDFKGIDIAFTSAGASISKEFADTVTKFGTIMIDNSSAFRMDDD
                     VPLVVPELNAKDALQRPRNIIANPNCTTIQMVVALKPIEDLSHITKVHVSTYQAASGA
                     GAAAMDELIAQHTQLVKGETATVEKFAYQLAYNLIPQVDVFTENGYTKEEMKMFNETR
                     KIMHSDVLVSATCVRVPVLRAHSESIWVETERAISLEEARNAFARAQGIVLQDDPANK
                     SYPMPLFVSGKNPVYVGRIRKDLTNPKGLTFWAVSDQIKKGAALNAVQIAEYLLTQAR
                     "
     gene            1931..2887
                     /locus_tag="PKY68_05190"
     CDS             1931..2887
                     /locus_tag="PKY68_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOH24466.1"
                     /translation="MHKCIFFSFSLLTIFFSCRPSVELQEEFGLFPVDTASAGSFYLS
                     LNEILFDPVPQSCDYVEIVNRSPFSVDLSEIRICNRNENGVLGSPKPLSRERFILDPG
                     AYCLLTSDWESLGRGFPLDTGLNYLIIKSLPSMPNDKGCVVLLDRQGNIIDECPYTKY
                     MHHCLISDREGIALEKIHPDLPSAEANSWLSAAADASYGTPGRINSQYRPLDTVGANE
                     GFFVEQDWLSPLGNSKYKQLVLGYSFADRRVANIKVYNRSGALLYSLAENALLGARGF
                     FVWQGVDSKGKVPDAGSYLIHIEHFNLKGDSKKQDMVCRILP"
     gene            2976..3926
                     /locus_tag="PKY68_05195"
     CDS             2976..3926
                     /locus_tag="PKY68_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOH24467.1"
                     /translation="MDWTQRTSLLIGDQALEKLQSSCVLIVGLGGVGAYAAEFLARAG
                     VGRLILVDGDKVQATNRNRQLPALCSTEGRFKVVVVAERLRDINPAIGLDCFPFFLEE
                     EEVGDLLFGRNRYDSRRAQQGIGKIFAGDDRKESLTPELSGQSTMVCDFVVDAIDSLG
                     PKAALIRSCLEHAVPLVSSMGAAAKLDPSQISTCDISKTHHCALARALRHRLAAFGIR
                     KGFPVVFSSEQAASKTRPSEAKSSEAKSSEAKTSEVKLSEARPFETKFSEVKSSEAEA
                     SVKVVPGTISYLPAAFACHLAAYTIKYLISENSSTNNQPD"
     gene            4049..4936
                     /gene="metF"
                     /locus_tag="PKY68_05200"
     CDS             4049..4936
                     /gene="metF"
                     /locus_tag="PKY68_05200"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOH24468.1"
                     /translation="MKIPDILRNKKTLSFEIFPPKRQEDTFVLIENTIKELSDLGPDF
                     ISVTYGALGNTLSNSVAIAKHIKQHTRSEALAHLTCVSSDKAQVDEICRELREANIKN
                     ILALRGDLPADPGMHHHDFRHASDLTAYISQRYGADFAFAGACYPEKHPEAISMKEDI
                     RHLKIKQDAGMDFFITQLFFDNEAFYRFLLEARKAGITIPIIPGIMPVSNYKQLDGMI
                     RVTGAAIPLQLRNYFDRYRYDKQALEEIGIIFTSHQILDLIANDVDGIHLYSMNKSSF
                     IKKLMANIAYVRSGFFAQE"
     gene            4905..>5513
                     /locus_tag="PKY68_05205"
     CDS             4905..>5513
                     /locus_tag="PKY68_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOH24469.1"
                     /translation="MCVPVSLLRSNALTYLGYSDSDLDEQTQEALHSALTHIAEWSAY
                     KQIYTRLKDLPEAPAFLQQESYVGLQGKLDTALLFAATLGLGIDRRIQSLSLNRLSEA
                     VVLNAVANAYLETRVELFIGEILPGGVLFCPGYAGTSPEGNRSVLKLLNAQKHLGIYL
                     KDSGLMIPEKSMAGIVVAGYSFSCRACSIRSKCDHLRQGKTCY"
BASE COUNT         1353 a         1320 c         1363 g         1477 t
ORIGIN      
        1 atcagcttgc gttcgaaagg tttgatgcag acagcggcat cgatgttggc gcgcaggtcc
       61 atgcctgccg aaaggctggt tgcgtattgg ggcagcgggt gtttggatcg gttgactatc
      121 tttacggtca tatctttatg gagacttaaa ttgctgtaaa ttcaataaat agcttaaaag
      181 tacatttaaa cggacaaaag tactaaaatt attgacaggc cgaataagag cttttaaaaa
      241 agcaggattg tccggggaat ttcttacatt tgcgggctgt ttgcccggca gcattttaat
      301 cgaaccaata tgaagcctgc ttatttgtat ctgttcctgc tcggagtgct tttttcgatc
      361 acttcctgtc tcgacgatcc tgaagtcacc gcctggcgca aagctaatga cgagtttatg
      421 ctaagcctca gggattcctc ggactttaag cctttggaat acaacgatcg tattttgtac
      481 ggcgacgaga actacaatta cctggatcat cctgtgagcg gtatttatta caaaaccctg
      541 gtagagggcc ggggtgcggt gcccttggtc ggacagacgg tacaagtgga gtacgaaggg
      601 cgcttatacg acggcactgt ttttgacagc ggcagcagaa ccacgctggt aggtaccgga
      661 gtcatcagcg gctggtcgga agtgctgtac agaataccgc aaggttccac ctgggaggtg
      721 gccattcctt atcacctggg gtacggagag gtaaaacacg gttccatacc ggcttactcc
      781 tgtcttattt tcacaatgga gttagtcagc atagtcgaag actaaaagca atcaacgtgc
      841 ctgagtgagt aggtattctg caatttgtac ggcgttcagg gcagctcctt tttttatctg
      901 atcacttaca gcccagaaag tcagtccctt aggattggtt aggtcttttc tgatgcgtcc
      961 cacgtaaacc gggttcttgc cggaaacaaa caagggcatg ggatagctct tgttggccgg
     1021 atcgtcctgc aaaacaatac cctgggcccg ggcaaaagca ttcctggctt cttcgaggct
     1081 aatggcgcgc tctgtttcga cccagatact ttcggaatga gcacgcaaaa ccggtacacg
     1141 cacacaagtg gcgcttacca acacgtcgga atgcataatt ttccgggttt cgttgaacat
     1201 cttcatttcc tctttggtat agccgttttc ggtgaaaaca tccacttgag gaatcaggtt
     1261 gtaggccagc tgataggcaa atttttccac ggtggcggtt tcgcccttta ccaactgggt
     1321 gtgttgagca atcaattcat ccatggcagc ggcaccggcc ccgctggcag cctgataggt
     1381 tgacacatgc acttttgtga tatgactcag gtcttcgatg ggtttgaggg ctaccaccat
     1441 ttgtatggtg gtgcaattcg gattggcaat aatatttcgg ggtctttgca aagcgtcttt
     1501 tgcattcagt tcaggcacga ccagcggcac atcgtcgtcc atgcgaaaag cgctggagtt
     1561 gtcgatcata atagtgccga atttggtaac ggtgtccgca aattcttttg aaatacttgc
     1621 tccggccgaa gtgaaggcta tgtctattcc cttgaagtcg tcgttatgtt ttagctcctt
     1681 gaccacgatt gattttccgc gaaaagaaaa ggttttgccg gcactacgtc cggatccgaa
     1741 caacaccagg tcgtccatgg ggaaattacg ttcatcaagt actttgagaa attcttgtcc
     1801 tacagcgccg ctggcgccaa caattgcaag tctcatgtta aaataaaatt aataaaagga
     1861 taatataata cataaaatgc ttacctttgc cgctgaattc atgccacgca aaggtagtaa
     1921 aaaagtgaag atgcacaagt gtatattctt ttcatttagt ctgctgacga ttttcttttc
     1981 ctgcagaccg tcggtggaac tgcaggaaga attcggcctt tttcccgttg ataccgcttc
     2041 ggccggctcc ttttatctga gcctgaacga gatattgttc gatccggtac cgcaatcttg
     2101 tgattacgtc gaaatcgtca accgttctcc gttctccgta gatctgtccg aaatacgtat
     2161 ctgcaaccgc aacgaaaatg gggtactggg cagtcccaaa cccctgagcc gtgagcgttt
     2221 tatactcgat ccgggtgctt attgtttgtt gacctccgat tgggaaagtc ttggaagggg
     2281 attcccgctg gacacaggat tgaattattt gattattaaa agcttacctt ccatgcccaa
     2341 cgataaaggt tgtgtggttt tgctggaccg gcagggaaac atcatagacg aatgccctta
     2401 cacaaaatat atgcatcact gcctgataag cgatagggag ggtattgctt tggagaagat
     2461 acatccagat ttaccttcgg cagaagcgaa ttcctggctt tcggctgcgg ccgatgcctc
     2521 ttacggaacg cccggcagga tcaattctca gtaccggcct ctggacaccg tcggtgcaaa
     2581 cgaaggtttt tttgtggaac aggattggct gtcgcccctg ggaaattcaa agtacaaaca
     2641 gttggtgttg ggctactctt tcgccgatag gcgtgtggct aatatcaagg tttacaaccg
     2701 cagcggggct ttgctttatt cgttggcaga aaatgccctg ctcggagccc ggggcttttt
     2761 cgtctggcag ggagtcgaca gcaaggggaa ggtgcccgac gcgggctctt atcttataca
     2821 tattgaacat ttcaacctga agggcgacag caaaaaacaa gacatggtat gccgtatttt
     2881 accctgaaaa ggctaacttt gtcgatcccg ttttattgaa acgattacgt acaccttacc
     2941 cggttgggac gatcccggcc aaaatcccgt aaattatgga ctggacacaa cgtacctctt
     3001 tgttaatcgg agatcaggcc cttgaaaaac tgcaaagttc ctgtgtgttg atagtaggtc
     3061 tgggaggagt gggcgcttat gcggccgaat ttctggcacg ggcaggtgta ggtcgtctga
     3121 tactcgtgga cggcgataag gtgcaggcta ccaaccgcaa ccggcaactg ccggccttgt
     3181 gcagcaccga aggtcgtttt aaggtagttg tagtggcgga gcgtttgagg gatattaatc
     3241 ctgccatcgg tctggattgt ttcccctttt ttctagagga agaagaggtc ggagatttgc
     3301 tctttggccg aaatcgctac gacagtcgtc gggcgcaaca gggaatcggc aaaatttttg
     3361 ccggtgacga taggaaagaa tcccttacac cggaactttc cgggcaatca acgatggttt
     3421 gcgattttgt ggtggacgct atcgacagcc tgggccctaa ggccgctctt atccgctcct
     3481 gtctggaaca cgctgttcca ttggtctcat cgatgggagc ggcagccaag ctcgatccct
     3541 cgcaaataag cacctgtgat atttccaaaa cacatcattg tgcgctggcc agggctctgc
     3601 gccaccgtct ggcggctttc ggcatacgta aaggtttccc tgtggtgttt tcttccgaac
     3661 aggcagcctc aaaaacccgg ccctccgaag ccaaatcctc cgaagccaaa tcctccgaag
     3721 cgaagacctc cgaagtcaaa ctctctgaag ccaggccctt cgaaactaaa ttttccgaag
     3781 tcaaatcctc cgaggcggaa gcctccgtaa aagtcgtgcc cggcaccatt tcctatttgc
     3841 cggcggcctt tgcctgccat ctggcggctt atacgattaa gtacctgatt tctgaaaatt
     3901 cttccacgaa taatcagccg gattgaaccg gcccggccgt atctaaaaag ttcagattgt
     3961 tgataaatag gcacaaaatg tattttccct tttgcactta tacgggaata ttgtctattt
     4021 ttgcgctttg ttttaattgc tctgatctat gaaaataccc gatatacttc ggaacaaaaa
     4081 aaccctttct ttcgagatat ttccacccaa aagacaggaa gatacttttg tacttatcga
     4141 aaacaccatc aaggaattat ccgacttggg ccccgatttt ataagcgtta cctacggggc
     4201 gctggggaat accctcagca attcggtagc catcgccaaa cacattaaac aacacacccg
     4261 ttcggaagcc ttggctcacc tcacctgtgt gtcgtccgat aaggcccagg tggacgaaat
     4321 ttgccgggaa ctcagggaag ccaacattaa aaatattctg gccctccggg gcgatttgcc
     4381 tgcagatccg ggcatgcacc atcacgattt tcggcatgcc tctgatctga ccgcttatat
     4441 ttcgcaacgt tacggagccg actttgcctt tgccggagcc tgttatccgg agaaacatcc
     4501 cgaagcaatt tccatgaaag aagatatccg gcacctcaag ataaaacagg acgcagggat
     4561 ggactttttt attacccaat tgttttttga caacgaggct ttttaccgtt ttttgctcga
     4621 agcccgaaag gccggtatca ccatcccgat tatcccgggt attatgcctg tgtccaatta
     4681 caagcagctc gatggcatga tacgagttac cggagccgcc atacccttgc aactgcgtaa
     4741 ttactttgat cgttaccgtt acgacaaaca agccctcgaa gaaatcggca ttatttttac
     4801 cagccatcag atcctcgacc ttattgcaaa cgatgtggac ggtattcatc tgtactcgat
     4861 gaacaagagc agttttatca aaaaattaat ggccaacatt gcctatgtgc gttccggttt
     4921 ctttgctcag gagtaatgcc ctgacttatc tgggttattc cgacagtgat ttggatgagc
     4981 aaacgcagga ggcccttcat tcggccctaa ctcatatagc tgagtggtct gcgtacaaac
     5041 aaatttacac tcgcctgaaa gatttgcccg aagcaccggc ttttttgcag caggaatcct
     5101 acgtgggctt gcagggaaag ctggatacgg ctttgttgtt tgccgccacc ctcggtctag
     5161 gcatcgatcg gcgtatacag agcctttcgt taaacaggct ttcggaagct gtggtgttga
     5221 atgccgtcgc caatgcctat ctcgaaacaa gagttgagtt gtttatcggg gagatattgc
     5281 ccggaggagt cttgttttgt ccgggttacg ccggtacttc gcccgaaggc aatcgctcag
     5341 tgttgaagct gctcaatgcc caaaagcacc tgggtattta cctgaaagat tccggactga
     5401 tgattcccga aaaatctatg gccgggattg ttgttgccgg ttatagtttc agttgtaggg
     5461 cttgcagcat acgcagcaaa tgcgaccatc ttaggcaggg taaaacctgt tac
//
LOCUS       DAOSGL010000134         5510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13085_length_5510_cov_3.817415, whole genome shotgun sequence.
ACCESSION   DAOSGL010000134 DAOSGL010000000
VERSION     DAOSGL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13085_length_5510_cov_3.817415"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PKY68_05210"
     CDS             <1..423
                     /locus_tag="PKY68_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH24470.1"
                     /translation="AETAADTPEPKTTQELSAEQEKLFSSGVTRERRGVIAFTFASAE
                     LTKDSAPAVLWMANNLKSGEWKALTIYGNADSTGEEAYNMILSIKRADAVKEALVRQG
                     ISELRIRTVGNGENKPFATNETKEGRRLNRRIDLEIVE"
     gene            494..2632
                     /locus_tag="PKY68_05215"
     CDS             494..2632
                     /locus_tag="PKY68_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOH24471.1"
                     /translation="MKADAMRYIKTLCYICLCSLFVFLSSLPLQAQKALYSDQFPLQD
                     VRLLDGPFKRAFDLNVQTLLKYDMPRLLQPFYKQAGLDNGARAFGNWAGLDGHVGGHY
                     LSALSIHYAASQDPYTRGLLKARIDSMIACLKTCQEAAVQLDPKMEHYLGGVPDSKNL
                     WTRFAQGDFSIYNRTWVAWYNVHKMFAGLRDAWLYAGDSTALQMFFDYCDWALAITSG
                     LSDAQMQTMLDIEHGGMNEVLADAYRLSGDLKYLNAAKRFTHRLFLDGMANENARFLD
                     NRHANTQVPKFVGIQRIYELSPSLLNYAKAAEFFWKDVVYNRSLALGGNSRSEHFIAP
                     SACGDYVTYREGPETCNTYNMLKLTENLFRLNQKAAYADFYEQALYNHILSTQHPEHG
                     GYVYFTSARPRHYRVYSQVNQAMWCCVGSGMENHGKYNQFVYTHRGDSLYVNLFIPTE
                     LQWRDKGVVITQETAFPYETSSRLSIRIPGGGSRDFSLWLRHPYWADSSAYCLRINGE
                     IVAHNSRPQTYVCISRSWHHGDVLSIDLPMKVHYKTLPNVNEYLALMYGPILLGANVS
                     TDRLDGLLAAEGRMDHVAEGPLLPLNQAPVLVGNRDSLAQAVHLVDSATLSFEVTDFL
                     KHNYDTTYQHLKLQPFYSLHDARYMMYWMQLRPDEYREIYDLLNREEQERLALEARTV
                     DQIATGEQQHDQNHHVEGVNTGVGVYKNTY"
     gene            2669..3667
                     /locus_tag="PKY68_05220"
     CDS             2669..3667
                     /locus_tag="PKY68_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24472.1"
                     /translation="MSTLGVDTLVLRATYWGYESGNRTFDILVEGDTIARENLVGKWN
                     ISEFRSVDYEIPVALTAGKSSIRVTFDALEGHVAGGLYHLRLMSDPGRAGRLRYEQVL
                     VADIDAGNSASESKYRVFVSGSSTGSHQGETWRDAYNGGYIRYTIPLDGQDSMFLRVR
                     YWGNEGLSREFDMLIDEVVFASESLVGKWNVNAFQEVDYNIPSHLFKGKSKINLKFRA
                     RPGNYAGGLFYVWLYALQAQEPDALAYECEDSREMDVLITDGMLRLRLPLEDRGLNNS
                     LRIFGMNGSLMYSGTIREEVRDEVQVFDLRRLPHPQCYVLLCSNEKGSRAQKLMIP"
     gene            3820..4383
                     /locus_tag="PKY68_05225"
     CDS             3820..4383
                     /locus_tag="PKY68_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOH24473.1"
                     /translation="MTELVEQYPYLLPLLIFFGRIFDVTLGTLRIIFVSKGKRTIAPV
                     IGFFEVFIWIVIISQILSHANDLISYLSYAAGYAAGTYAGMLIESKLAFGVLLYRVYT
                     KESGAELVQMLSKDGFGATMIRGEGSVGKVDVVETVVARQQMKKVERVINNFDEHAFY
                     VTEDVLTAQNGIFPKTANVFKRWRPGK"
     gene            4397..5281
                     /locus_tag="PKY68_05230"
     CDS             4397..5281
                     /locus_tag="PKY68_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH24474.1"
                     /translation="MMNHSRNPYSTLRESIINLGYEWQEKDYFFCHGLLPKIDNTITD
                     MKEYVGVLLCTDGDIDISINKVDYNLTASTLLIAHPLSIVHIIRSKRQYKGILLFVAR
                     DFLAKNIVNAQLAKPFRQISHHQYTLTNLNREQQKILIDIYKLIYRKRATKQSVFQLE
                     TLRNLFLVFICEVAEIFLESKEVESHSTRNEEIADAFFNLLLQPVKITNKTAFFADQL
                     NISPKHLIKAVKTIAGKAPGVFIHEKITDDAKIMLTDKKLSIGQVSEKLGFSETSSFS
                     KFFKKQTGTSPSLFREQF"
BASE COUNT         1492 a         1239 c         1344 g         1435 t
ORIGIN      
        1 gctgaaaccg ctgcggatac accggagcca aaaacaaccc aagagttaag tgccgaacag
       61 gaaaaactct tcagttccgg agtcactcgc gagcgcagag gagtgattgc tttcaccttt
      121 gcttcggcgg aattaaccaa agattcggct cctgccgtcc tttggatggc caataatctc
      181 aagagcggag agtggaaagc cctgaccatt tacggtaatg ccgacagcac aggcgaagaa
      241 gcctacaata tgattctttc gatcaagcgg gccgatgccg tcaaagaggc attggtccgg
      301 caagggatat ccgaactgcg catccgcacc gtaggcaacg gcgaaaataa accttttgcc
      361 accaacgaaa caaaagaggg acgccggctt aaccggcgca tcgatctgga gattgtggaa
      421 taatggaatt tcgtttgagg attcgccgga attaaggtac atttgtgttc tttaaatttt
      481 aaattcattt tctatgaaag cagacgctat gcgttatatt aaaacgctgt gttatatttg
      541 tttatgctcg ttatttgtat tcctttcctc tttgcctctg caggcccaaa aagctttgta
      601 ttccgatcag tttcctttac aggatgttcg tttgctcgac ggccctttta agcgggcctt
      661 tgacctgaat gttcaaaccc tgctgaagta cgatatgcct cgtttgctgc agccctttta
      721 caaacaagca ggtctcgaca acggagcccg ggctttcggc aactgggcgg gtttggacgg
      781 tcacgtgggc ggacattacc tttcggccct gtccattcat tatgccgcct cgcaagatcc
      841 ctacaccagg ggcctgctca aagccagaat agacagcatg attgcctgcc tgaaaacatg
      901 tcaggaggcc gccgttcaac tggatcccaa aatggagcat tacctgggag gtgttcccga
      961 cagcaaaaac ctctggactc gttttgccca gggtgatttc agcatttata accggacttg
     1021 ggtggcctgg tataatgtgc acaagatgtt tgccggtttg cgcgatgcct ggctgtatgc
     1081 gggcgacagc accgctttgc aaatgttttt tgattattgc gactgggccc ttgcgattac
     1141 ttcaggattg tctgatgctc agatgcagac catgctggat attgagcatg gaggcatgaa
     1201 cgaggtgctg gccgacgctt accggctcag cggcgacctc aagtatttga acgcagccaa
     1261 acggtttact catagattat tcctcgatgg catggccaac gagaatgcac gttttctcga
     1321 taacaggcac gccaataccc aagtacctaa atttgttgga attcagcgaa tttatgagct
     1381 ctcgccctcc ttgctcaact acgccaaggc ggccgaattt ttttggaaag acgttgttta
     1441 taatcgttct ctggctttag gcggcaacag ccgcagcgag cattttattg caccttcggc
     1501 ctgcggtgat tacgtcactt accgcgaagg ccctgaaact tgcaatactt acaatatgct
     1561 caaactgacc gaaaaccttt ttcgcctcaa tcaaaaggca gcttacgccg atttttacga
     1621 acaggcgctc tacaaccata ttttatcgac tcaacacccc gaacacgggg gctatgtgta
     1681 ttttacttcg gcccggcccc ggcattaccg ggtgtattcg caggtgaatc aggctatgtg
     1741 gtgctgcgtg ggaagcggta tggaaaatca cggcaaatac aaccagtttg tttatactca
     1801 tcgtggcgat tccttgtacg tgaatttgtt tatccccacg gaacttcaat ggcgcgacaa
     1861 aggagtggtt attacccagg aaacggcttt tccctatgaa acaagcagcc gcctgagcat
     1921 ccggattccc gggggcggat cacgggattt cagcctttgg ctgcgtcatc cttattgggc
     1981 cgacagcagc gcctattgct tgcgtataaa cggggaaatc gttgctcaca acagtcgtcc
     2041 gcaaacttat gtttgcatca gccgaagctg gcaccacggc gatgtgctga gtatagacct
     2101 gccaatgaaa gtccattata aaactctgcc caatgtgaac gaatacctgg ctttgatgta
     2161 cggccccatt ttgttgggag ccaatgtaag taccgaccgg ctggacggtt tgctggccgc
     2221 cgagggccgt atggatcacg tggccgaagg gcccctgctg cccttgaatc aggcgccggt
     2281 attggtaggg aaccgcgatt cccttgctca ggccgtacat ttggtcgatt cggcgacatt
     2341 gagctttgag gtaactgatt ttttgaaaca taactacgat accacctatc agcatttgaa
     2401 gttgcaacct ttttattccc tgcacgatgc ccgttacatg atgtactgga tgcagttgcg
     2461 ccccgatgaa taccgggaaa tttacgatct gctgaaccgc gaagagcagg agcgtctggc
     2521 tctggaagcc cgcacggtag accagattgc caccggcgag caacaacacg accagaatca
     2581 ccatgtcgag ggagtgaata ccggggtagg ggtgtataaa aatacttatt agagagaagc
     2641 cactcaggga ggctggttca gttatgaaat gagcactctg ggcgtcgata ccctggtgtt
     2701 gagagctact tattggggtt acgaaagcgg taacagaact tttgacattc tggtcgaagg
     2761 cgataccatt gcccgcgaaa acctggtcgg gaaatggaat atttcggagt tcagatcggt
     2821 ggattatgaa attccggtag ccctgacggc aggcaaaagc agtatcagag ttacctttga
     2881 cgccttagaa gggcatgtgg ccggaggctt gtaccacctt cgcctgatgt ccgatccggg
     2941 tagggcaggc agactgcgct atgagcaagt gttggtggcg gacatcgatg caggcaattc
     3001 cgcttcggaa agcaaataca gggttttcgt atcgggttcc agcaccggca gccatcaagg
     3061 cgagacctgg agagatgcct acaacggagg atatattcgt tacaccattc cactcgacgg
     3121 tcaggacagt atgtttttgc gtgtgcgcta ctggggcaac gaaggcctga gtcgcgaatt
     3181 cgatatgttg atagacgaag tggtgtttgc cagcgaaagc ctggtgggca aatggaatgt
     3241 aaatgctttt caggaagttg attacaacat cccttcgcac ttgtttaaag ggaaaagcaa
     3301 gatcaacctg aaattcaggg caaggccggg caattacgcc ggtggattgt tttacgtttg
     3361 gctctatgcc ctgcaagctc aggaaccgga cgctctggct tacgagtgtg aagacagcag
     3421 ggaaatggat gttttaatca ccgacggcat gcttcgactc aggctgcccc tcgaagaccg
     3481 gggtttaaac aacagtttgc gtatttttgg gatgaacggt tcgctgatgt attcaggaac
     3541 aattcgggag gaggttaggg acgaggttca agtgtttgac cttcgccgac tgccccatcc
     3601 gcaatgctat gtgttgctct gttccaacga aaaaggctcc agagctcaaa agctgatgat
     3661 cccctaaagt tttaccaatt tatccaataa gcagttacga cgatacgaat ttacggcggt
     3721 tattcggccc gcccggcaag tgaccaggcg atttacaaac aagtgagtac aagtagattt
     3781 taagacaaaa tttaagataa atttttaaga caaaataaaa tgacagaatt agtagaacaa
     3841 tatccttatt tgttgcctct tctgattttc ttcgggagaa tttttgacgt tacattaggt
     3901 accttgagaa taatctttgt ctccaaaggg aaaaggacta tagcaccggt tatcggtttc
     3961 tttgaggtat ttatatggat agtaattatt tcgcagattc tttctcatgc caacgatttg
     4021 atatcttatc tttcctacgc cgccggttac gccgccggta cctacgcagg tatgctgata
     4081 gagagtaaat tggccttcgg cgtgttgtta tacagagtgt acaccaaaga gagtggcgcc
     4141 gagcttgtac aaatgctgag caaagacgga ttcggggcta ccatgattcg cggcgaaggc
     4201 tcggttggta aggtggatgt ggtagaaacg gtagtggcac ggcagcaaat gaaaaaggta
     4261 gaaagggtga taaataattt cgacgaacac gccttctacg taaccgaaga tgtccttaca
     4321 gcccaaaacg gcattttccc caaaaccgcc aatgtcttta aacgctggag accggggaag
     4381 taagttaagt taaataatga tgaatcactc acgcaatcca tattctacat tgcgagaatc
     4441 catcataaat ctgggttacg aatggcagga aaaagattat tttttctgcc acggtctgct
     4501 tccgaaaatc gacaacacga ttaccgacat gaaagagtat gtgggtgtgt tgttgtgtac
     4561 ggatggagat attgatataa gtatcaataa ggtagattat aacctgacgg catcgacttt
     4621 gcttatcgca catcctttgt ccattgtaca tattatacga tcgaaaagac aatacaaagg
     4681 cattcttttg tttgtggcgc gagacttttt ggctaaaaac attgtaaatg ctcaacttgc
     4741 aaaacctttt cgtcaaatca gccatcacca atacacactt accaacctta acagagaaca
     4801 gcaaaagatt ctgatcgata tttacaaatt gatttacaga aaaagagcaa ccaaacaatc
     4861 ggtgtttcag ctggaaacct tgcgcaattt gtttttggtt tttatttgcg aagtggctga
     4921 aattttcctg gaaagtaaag aagtggaaag ccattcaaca agaaacgaag aaattgccga
     4981 tgcttttttc aatttacttt tgcagccggt aaaaataacg aacaaaacgg ccttctttgc
     5041 cgaccaatta aatatttccc ccaaacatct gattaaagcg gttaaaacaa tagccggaaa
     5101 agctcccggc gtttttatcc atgagaaaat taccgacgat gccaagataa tgttgaccga
     5161 taaaaaactg tctatcggtc aggtttcaga aaaattggga ttttccgaaa cctcgtcgtt
     5221 cagcaaattt tttaaaaagc aaacaggcac ctctccatcc ttatttaggg agcagttcta
     5281 aaagagaatt ttaggcaact acatgtgaat ttcggacaac gaaatgcctg ctgtgtttgt
     5341 taattttgtg ggttataatt cgcattattc aggacttgtt tcgtaagtac ccaactgctg
     5401 tgtttttgat atgtgttttg agacagcccc ggaaaacatt acaaagcaaa gcattataat
     5461 ttattgaaat ggataagaat aaaatcaccg acgatccttc acaaagaccc
//
LOCUS       DAOSGL010000135         5502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13109_length_5502_cov_4.135855, whole genome shotgun sequence.
ACCESSION   DAOSGL010000135 DAOSGL010000000
VERSION     DAOSGL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13109_length_5502_cov_4.135855"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKY68_05235"
     CDS             complement(<1..658)
                     /locus_tag="PKY68_05235"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOH24475.1"
                     /translation="MNKYALISAKVSRFLLLTALILFVSCKDKTIRNPLAPTAVGTPW
                     EVLVVLEKDLWDSPAGESIFSLLREDVPGLPQPESRFEISRCDDDEFTGILKPVRNII
                     QIEISDKYSEPKLFFYRDQWASNQYVLRIVAPSEELFVFFVERNAEIIISYFELAERE
                     RSLQYIRKTHNRAFSEKVYEQFGFKILVPGFMKLSKQADQFFWASNYQNKKRQDMVIY
                     T"
     gene            complement(786..1808)
                     /gene="rlmN"
                     /locus_tag="PKY68_05240"
     CDS             complement(786..1808)
                     /gene="rlmN"
                     /locus_tag="PKY68_05240"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOH24476.1"
                     /translation="MDKYILLGKSFEELKTWTLAQGLPSYTASQLFEWIYRKPAKSFD
                     EMTNISLQNRALLKEKSELGIYPPIQKSTSKDGTIKYLFPSREGYGVEAVYIPESDRA
                     TLCVSSQAGCKMNCVFCMTGYMGFKAQLSAGEILNQILSLPQSASLSNIVFMGMGEPA
                     DNIDAVIQSLNLLTSPRAMAWSPRRITVSSIGVLPGLKRLLDETRCHIAISLHSTDAA
                     QRLAWMPAQKTWPLLSTIELLKTYDFSKQRRLSFEYIMFKNLNDRIEDAKALLRMLNG
                     LRCRLNLIRFHAFPESELRGSDEEQIQSFATYLNNKGLQTTIRQSRGEDVQAACGMLS
                     SQYKSG"
     gene            complement(1908..4049)
                     /locus_tag="PKY68_05245"
     CDS             complement(1908..4049)
                     /locus_tag="PKY68_05245"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOH24477.1"
                     /translation="MATLQKIRSQAVLLLIVVGVALFAFIIGDFLTSGSTIMRQSQEN
                     IAEINGSPLNYKEYDARILEMEEVYKMQTGQNNLQSDIMHQIRESVFETIVRERLLDD
                     QAQDLGVSVTAKELYDMVNGQNVHYMVQQMPFFQNPETGRFDRNMMMNFLRTIQQEDF
                     SMYSAEAQEQIKQMKNYWLFWENNLKYVRLDEKMVNMVTKAVGANSLDAKDEFEASRR
                     SVDFEYASKSYASVPDSSFKVSLKELSRRYEEQKERFKQEPYRSAAYVVVDILPSQED
                     NDAIKARIDKIATDFSTEENVATTVNSESDLPYVDCYIANNQFSGSLKDFVQQAAVGE
                     VKGPYFEDNAWIMLRMMGKINRSDSVKARQIFISNAGDRKAAERLADSLLNVLRRGGD
                     FVALAGQYSQDRTASDGGEMGWFREIDAYSGIGPEFAEACFTAYKNQFLTVNSKFGWH
                     VVQIQDKTKAVAKSKVAQLYLAVTPSSKTYSAEYNKLSKLMAGNSKAEDFILAAREAG
                     YQVSNAAVVKSTDNSIGNVPEMRQAVRYVFNNKLGDVSTVLENSNNQFVVVAVTGIYD
                     NKYLSLDQVRDELSREIINEKKAASIIASLQVDDNTVLSDLAKSHSMQTDTVRLVNFS
                     MRRITGLGEEPALLAAVMSAAKGELSQPVAGKNAVYVFRVLSQVEPEIEFDVVAEKRN
                     QDMNNNYRLTYQAYQALRDAAKVEDNRIRFY"
     gene            complement(4195..4791)
                     /gene="lptC"
                     /locus_tag="PKY68_05250"
     CDS             complement(4195..4791)
                     /gene="lptC"
                     /locus_tag="PKY68_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOH24478.1"
                     /translation="MLLRSLFGKYSGITVASFVTVILFLSLSCKREKADILPNFSSRD
                     SVPGMRADSIVTLISDSGRIRYKVITASWLVYDQLPDPYWFFPEKIYFERFDDSLRIE
                     SIVKSDTARYYTNRKLWELKKNVQVMNLAGEKFETPLLYWDQNLHKIYSDSFIRIEQK
                     DWISTGIGFESNESMTKYSILNSQGNFPIDPEEENSDQ"
     gene            complement(4794..>5502)
                     /locus_tag="PKY68_05255"
     CDS             complement(4794..>5502)
                     /locus_tag="PKY68_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24479.1"
                     /translation="VGIKNGVDVLFSNSGAADCETLTALFAERIEQNKDNQNFLNSVI
                     SLFKRAKCTDTDTYFLASEYRHDVNPTAESAEGCAYQAIKKEDYKKAAEYLKEAIELE
                     TNPDGKADYAFLAASALFAGKSYEQARSMALQAAEYRENFGAPYVLIAKMYAGSVDYF
                     DDPILKRAVYWAAVDKLQKAKAVDPSVSDEVDELIARYKEQYPDTEDIFMHPDLTVGK
                     MFRIGGWIQENTLVRNK"
BASE COUNT         1509 a         1231 c         1121 g         1641 t
ORIGIN      
        1 aggtataaat taccatgtcc tgccgttttt tgttttggta attcgaggcc caaaaaaatt
       61 ggtcagcctg cttgcttaac ttcataaaac ccggaaccaa tattttaaac ccaaattgtt
      121 catacacttt ctcagaaaat gcgcggttgt gcgttttgcg aatgtattgc aaggaacgtt
      181 ccctttcggc taattcaaaa taagaaataa ttatctctgc atttctttca acaaaaaaga
      241 caaacaattc ctcactgggt gccacaattc tcaacacata ttggttactg gcccattggt
      301 cgcggtaaaa aaacaattta ggttccgaat acttatcgga gatttcaatc tgaataatat
      361 tacgtaccgg ttttaaaatg ccggtaaact catcgtcgtc gcaacgggaa atctcaaaac
      421 gcgactcagg ctggggtaaa cccggaacgt cttctctgag caaagaaaaa atgctttcac
      481 ctgccggact gtcccaaagg tccttttcca agaccacaag cacctcccag ggggtaccaa
      541 ctgccgtggg agccaaagga ttacgtatcg ttttatcctt acacgatacg aaaagaatca
      601 gggccgttaa aagcaaaaac cggcttactt tagcggaaat aagggcatat ttgttcatac
      661 ctaatggatt taatgcaaca aatgacaaaa atagcatact tttacggtat atccgtagtt
      721 tttcctgatt ttttaaccaa gaatctgact ttttgtcatc aaagaggtgg aactcagatt
      781 gcgagttagc cagacttata ctggctggac aacatgccgc aggcagcttg cacgtcttct
      841 ccccgggatt gccttattgt agtttgtaaa cccttattat tgaggtatgt tgcaaacgat
      901 tgtatctgtt cttcgtccga tcctctgagt tcgctttcgg gaaaagcatg aaaacgaatt
      961 aaattaagtc ggcacctgag tccgttcaac atacgaagca gggctttggc atcttcgatg
     1021 cgatcgttga gatttttgaa cataatatat tcaaaggata gacggcgttg tttactgaaa
     1081 tcgtaagtct tgagcaattc tatggtcgaa agtaagggcc aggttttttg ggccggcatc
     1141 caggccaatc gttgagcggc atcggtcgaa tgcagactta tagcaatatg gcatctggtt
     1201 tcgtccaaca gacgctttaa acctggcagt actccgatgc tcgaaaccgt aatccgtctt
     1261 gggctccagg ccattgcccg gggtgaagtc aacagattca ggctttgaat aactgcatcg
     1321 atattatctg caggttctcc catacccata aagacaatat tactcaaaga agcactttgg
     1381 ggcagggata aaatctgatt gaggatttcg cccgccgata gctgagcctt gaagcccatg
     1441 tagcctgtca tacaaaaaac acaattcatt ttgcatccgg cttgcgacga aacacagagc
     1501 gtggcacgat cgctttccgg aatataaacc gcctcgacac cgtagccttc cctacttgga
     1561 aaaagatatt taattgttcc gtctttcgaa gtgcttttct gtatgggcgg atatatgcca
     1621 agctcggact tttcttttag cagagctctg ttttgaagcg agatgttagt catctcgtca
     1681 aaactcttag ccggtttacg gtagatccat tcaaacaatt gagaagccgt atacgaaggc
     1741 agcccctgag caagagtcca ggtttttaat tcttcgaatg acttgcctaa caatatgtac
     1801 ttatccattg ccgtaaaaaa agcggtgcct cataagtacc caactgctcc gtttggatac
     1861 ttctaagaca cctgctgttt ttcaattgaa tagttgtttt gagagaatca ataaaaacgt
     1921 attcgattgt cttcgacttt tgcggcatca cgcagggctt ggtaagcctg ataggtcaat
     1981 cgatagttgt tgttcatatc ctgatttctt ttttctgcaa ccacgtcaaa ctcaatctca
     2041 ggttcaacct gactgagcac cctgaataca tagaccgcat ttttaccggc aaccggttgg
     2101 gacaattcac ctttggcagc gctcattaca gcagctaaca gagcgggttc ttcgcccaat
     2161 ccggtaattc ttcgcatgga aaagtttacc aaacgcaccg tgtcggtttg catggagtgg
     2221 gatttggcca aatcagacaa aacagtatta tcgtctactt gcagagaagc aatgatagag
     2281 gcggctttct tttcattaat gatttcacgg cttagctcat cgcgaacctg gtccagactt
     2341 aagtatttgt tatcgtaaat accggtaaca gccaccacca caaactgatt gttgctgttt
     2401 tccaatacag tagatacgtc tcccaactta ttgttgaata cataacggac ggcctgacgc
     2461 atttcgggaa cattaccgat gctgttatcc gtacttttca ccacagcggc attcgaaacc
     2521 tggtagccgg cctccctggc agccaagata aaatcttctg ctttggaatt tcctgccatg
     2581 agtttactaa gtttgttgta ttctgcacta taggttttgg agcttggcgt taccgccaaa
     2641 tacaattgag ctaccttgga tttagctacc gctttggttt tatcctgaat ttgtactaca
     2701 tgccaaccaa attttgagtt tacggttaaa aattggtttt tataagccgt gaagcaagct
     2761 tcggcaaatt ccggacctat tccgctgtaa gcgtcaattt cgcggaacca tcccatctct
     2821 cccccgtcgc tagctgtccg gtcctgggag tattgtcctg ccagtgccac aaaatcccct
     2881 cccctacgta aaacattgag caagctgtcg gccaatcgtt ctgcagcttt gcgatcgccg
     2941 gcgttgctga taaagatttg ccgggctttt acagaatcgg accggttgat tttgcccatc
     3001 atacggagca taatccaggc attatcttca aaataagggc ctttcacttc tccaacagcg
     3061 gcttgttgca caaaatcttt cagacttccg gagaattggt tgttggcaat ataacaatct
     3121 acataaggca gatcagactc agaattgacg gtagtagcga cgttttcttc ggtgctgaag
     3181 tccgttgcta ttttatctat tctggctttg atggcatcgt tgtcttcctg actcggaaga
     3241 atatcaacta ccacataggc agctgaacgg tagggttcct gtttgaagcg ttctttttgt
     3301 tcctcgtaac gacgactgag ttctttaaga gaaactttga agcttgaatc gggaacgctg
     3361 gcataagact tggaagcata ctcaaaatcg accgaacgac ggctcgcttc aaactcatct
     3421 ttggcatcca ggctgtttgc tcctacggct ttggtaacca tgttcaccat tttttcatct
     3481 aaacggacgt atttaagatt gttttcccag aacagccagt aatttttcat ttgtttgatt
     3541 tgttcctgag cctcggcaga atacatggaa aaatcttctt gctgtatggt tctgagaaaa
     3601 ttcatcatca tattgcggtc aaaacgtccg gtttcgggat tttgaaaaaa gggcatctgt
     3661 tgtaccatgt agtgaacatt ttgcccgttt accatatcgt aaagttcttt tgcggtaaca
     3721 cttacaccca aatcttgggc ttgatcgtcc aaaaggcgtt cgcgaacaat ggtttcaaaa
     3781 accgattctc ttatttgatg cataatatcc gactgcaggt tgttttgtcc ggtttgcatt
     3841 ttatacactt cttccatctc taaaatacgg gcatcgtatt ccttgtaatt gagtggactg
     3901 ccgttaattt cggcaatgtt ttcctgcgat tgcctcataa tggtagaacc ggaagttaaa
     3961 aagtctccga taataaacgc aaataatgca acaccgacca cgatcaacaa aaggacggcc
     4021 tgacttctga ttttttgtaa ggttgccata tactatttgt aggttataat tgtgttcttt
     4081 aaactattcc cgagtaccat ttacaaaaca gtatcgaaaa atcccgcaaa tgtacaaaaa
     4141 aatatgaaat aacacgcagc aaaaccaatt aaagaacagc cctgcacatt aaaatcattg
     4201 gtcggaattt tcttcttcgg gatctattgg aaaattacct tgggaattga gaatactata
     4261 ttttgtcata gattcgttgg attcaaaacc tatgccggtg gatatccaat ctttttgttc
     4321 aatgcgaata aaagaatctg aataaatttt atgcaggttt tgatcccaat acaacaaggg
     4381 ggtctcaaac ttttcaccgg ccaggttcat cacctgtaca ttttttttga gctcccagag
     4441 tttcctgttg gtataataac gagccgtatc gcttttcacg atagattcaa tgcgtaaact
     4501 gtcgtcaaag cgttcgaaat atattttttc gggaaaaaac cagtaaggat cgggaagctg
     4561 atcgtaaact agccaggaag cagtaattac tttgtaacgg atgcgacctg agtctgaaat
     4621 caatgtcaca atggaatcgg ccctcatacc gggcacagaa tccctcgagc tgaagttggg
     4681 cagaatatcc gctttttctc ttttacagga aagggataaa aacagtataa cagttacaaa
     4741 agatgcaact gttatacctg aatactttcc aaataagctc cttaaaagca tctttatttg
     4801 ttgcggacaa gggtattttc ctgtatccag cctccgattc tgaacatttt tcctacagtg
     4861 agatccggat gcataaaaat atcttcggta tcgggatact gttctttata ccttgctatc
     4921 aattcatcta cttcatcact cacactggga tctaccgctt tggctttttg cagtttatca
     4981 accgctgccc aataaacggc tcttttaaga ataggatcat caaaatagtc gacactgccg
     5041 gcgtacattt tggcgatcag cacataggga gctccgaagt tttcccggta ttctgcagct
     5101 tgcaaagcca tggagcgggc ttgttcatag ctcttaccgg caaacagtgc actggcagcc
     5161 aaaaaagcat aatcggcttt cccatccgga tttgtttcta attcaatggc ttctttcaga
     5221 tattcggcgg cttttttgta atcttccttt ttgattgcct ggtaagcaca accttctgcc
     5281 gattctgccg ttgggttaac atcgtgtctg tattccgaag caagaaaata agtatctgta
     5341 tctgtgcact tagccctctt gaataaagaa atgacgctat tcaagaaatt ctgattgtct
     5401 ttgttctgct caatacgttc tgcaaagaga gcggtcagtg tttcacagtc ggctgctcct
     5461 gaatttgaaa agagcacatc cacaccattc ttaatgccta cg
//
LOCUS       DAOSGL010000136         5491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13145_length_5491_cov_4.053164, whole genome shotgun sequence.
ACCESSION   DAOSGL010000136 DAOSGL010000000
VERSION     DAOSGL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13145_length_5491_cov_4.053164"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PKY68_05260"
     CDS             complement(<1..896)
                     /locus_tag="PKY68_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOH24480.1"
                     /translation="MKDQFNIYSSDSQASKVLPLSPEEFDFEAYAEYEARLNQGCKKF
                     WEAPSGVMVYRRMRVKEVFAEDSADWQKSLNWQLGALQQSMGYKADIPNFLEPWYGIG
                     TIASAFGFGYRWEQGQAPALDGKFEQLEHLLQADFKPVAQTAIGSHSLEMTEYFLEQT
                     RGRLPMSWCDVQSPLNILCNLVDSNMLFPEFYLNPEAVEQALDKITDLLIEFTKVQTR
                     LIGPALVKPGHGFASSRFFDGFGMSDDNIVMLPPDLYNDFAVPRMVRAGRPFGGPVFH
                     SCGNWSDKKEDIVRIDGLRMADG"
     gene            complement(943..3009)
                     /gene="metG"
                     /locus_tag="PKY68_05265"
     CDS             complement(943..3009)
                     /gene="metG"
                     /locus_tag="PKY68_05265"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOH24481.1"
                     /translation="MTRQFKRTTVTTALPYANGPVHIGHLAGVYVPADIYVRYLRLKG
                     QDVLFIGGSDEHGVPITLKAKKEGVSPQAIVDRYHHIIKKSFEDFGISFDIYSRTTSA
                     IHHKTASAFFDKLNRQGSFVEKTSEQYYDEEAGQFLADRYITGTCPHCSNENAYGDQC
                     ESCGNSLSPNELIHPRSAISGSKPVMKATKHWYLPLDQYEAFLKQWILEEHKEWKSNV
                     YGQCKSWLDMGLQARAVSRDLDWGVPVPVEGAEGKVLYVWFDAPIGYISNTIELLPDS
                     WEKWWKDPETRLIHFIGKDNIVFHCIVFPAMLKAEGSFILPDNVPANEFLNLEDDKIS
                     TSQNWAVWLHEYLEDFPGKQDVLRYVLTANAPETKDNNFTWKDFQARNNNELVAVFGN
                     FVNRAMVLTHKYFQGKVPARGEPNDYDRQTMEEFTDVQNKVETLLDVFRFRDAQKEAM
                     NLARIGNKYLADTEPWKLAKTDLPRVGSILNLCLQIAANLAIVFEPFLPFTAQKLRRM
                     LGIDSFRWESLGRTDLLPVGHQLNPAELLFEKIEDDAINQQIQKLLNTKNNNKATREN
                     QAEAIWTPAPAKAPIDYDSFCKMDIRVGTILQAEAVPNTEKLMKMLVDTGLDQRTIVS
                     GVLEHFSPEELIGKQVPVLINLEPRKIRGIVSQGMILYAENDQKRFALMQPQAGKTTP
                     NGSIIC"
     gene            3489..3974
                     /locus_tag="PKY68_05270"
     CDS             3489..3974
                     /locus_tag="PKY68_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOH24482.1"
                     /translation="MRFLGNIEAKLDAKNRVFVPAAFRKVLDNAVFTKTDEGSQSRIL
                     YLRKDVYQNCIVVYPAPVWEEELSELRARLNKWNPQEQELYRQFMLEAEAVEMDPNGR
                     ILISKRLLLKTGIEKNVRFLGVDNTMEIWAKEALEQPRVDPELFKTRMQELMTKTKIP
                     E"
     gene            3982..4884
                     /gene="rsmH"
                     /locus_tag="PKY68_05275"
     CDS             3982..4884
                     /gene="rsmH"
                     /locus_tag="PKY68_05275"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOH24483.1"
                     /translation="METPYHIPVLLSESVDGLALKPGGIYVDATFGGGGHSRAILAQL
                     KGGRLYAFDQDEEALKNLPEDKRLQFVHGNFRFLSNFMRYYGEEQVDGILADLGVSSH
                     HFDQAGRGFSFRFDVNLDMRMNQRATLTAADILADYSKEELCRVFRVYGEFRQAARMA
                     GAVVKAREEQAVRTAGQLSDLLRPLLSRQAEKKDLARVFQALRIELNQEVGALEDLLT
                     QSLDILKPGGRLVVISYHSLEDRLVKNFMRTGNLEGEVKKDFYGNNLSPFILINNKVL
                     VPSPEEQELNPRSRSARLRIAEKR"
     gene            4888..5235
                     /locus_tag="PKY68_05280"
     CDS             4888..5235
                     /locus_tag="PKY68_05280"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOH24484.1"
                     /translation="MEQEEENKKTTKRFIFGEFIDGSFLDKNFFRKHAKLIFLIVVLL
                     FVYIGNHYSVIMKLAEINRLQQELNDVKYEALMYTSEFMQESRQSHIKSMVEEKGLEL
                     KELTEPPYTLKVK"
     gene            5265..>5491
                     /locus_tag="PKY68_05285"
     CDS             5265..>5491
                     /locus_tag="PKY68_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24485.1"
                     /translation="MDEQTGEDITRGIRKRYLIIVILCFSVMLFGIIFAAFHIQFAEG
                     AAWAEWKNKQQRENVVVPAGRGNIFSDEGLL"
BASE COUNT         1403 a         1132 c         1437 g         1519 t
ORIGIN      
        1 ccgtcggcca ttcggagccc gtctatacgg acaatgtctt cttttttgtc tgaccagttg
       61 ccgcaggagt ggaacaccgg acctccaaaa ggccggccgg cacggaccat tctcggtacg
      121 gcgaagtcgt tgtaaaggtc gggaggcaac ataacgatat tgtcgtcgct catgccaaag
      181 ccatcaaaaa acctgctcga agcaaaaccg tgtccgggct tgaccaaagc ggggcctatg
      241 agtctggtct gtaccttggt gaactcgatg agtaaatcgg tgatcttatc taaggcctgt
      301 tctaccgctt cgggattcag gtaaaattcc ggaaaaagca tgttagaatc gaccaagttg
      361 cacaggatat ttagggggga ctggacatcg caccacgaca tgggcaaacg cccccgggtc
      421 tgctccagaa agtattcggt catttcgagg ctgtgactgc cgatggctgt ttgggctacc
      481 ggtttgaaat cggcctgcag caaatgttcc aactgctcaa atttaccgtc cagggccggc
      541 gcctggcctt gttcccagcg atagccgaaa ccgaatgccg aggctatggt gcctataccg
      601 taccagggct ccaggaaatt gggaatgtcc gctttatagc ccatactttg ttgcagggct
      661 cccaactgcc aattaaggga tttctgccaa tcggcactgt cttcggcaaa gacttctttt
      721 acccgcatac ggcgatagac cattacaccg gagggagctt cccaaaactt cttacagcct
      781 tggtttaagc gggcttcgta ttcggcatag gcttcaaaat caaattcttc aggacttaag
      841 ggcagaactt tgctggcttg agagtcggaa gaataaatat tgaattggtc tttcattttt
      901 ctgttttatt gaaggagaga agctccgcac ggtgatcaga attcaacaaa tgatacttcc
      961 gttgggcgtt gttttgccgg cctggggttg cataagggca aatctttttt ggtcgttttc
     1021 ggcgtagagt atcatgccct gagaaacgat gcccctgatt tttcgcggtt caagattgat
     1081 caggaccgga acttgtttgc cgataagctc ctcgggactg aaatgttcca gtacgcccga
     1141 aacaatagtg cgttgatcca ggcccgtgtc taccagcatc ttcataagtt tttcggtgtt
     1201 gggaacagct tcggcctgca gtatggtacc aacgcgaatg tccattttgc agaaactgtc
     1261 gtaatcgatg ggggctttgg ccggggcagg ggtccagatt gcttctgctt ggttttcccg
     1321 tgtggcttta ttgttgtttt tggtgttgag cagtttttga atctgttgat ttatggcatc
     1381 gtcttctatc ttttcaaaga gcaattcggc gggattgagc tgatgaccca caggcagcag
     1441 atcggtacgg cccagcgatt cccagcggaa gctgtctatg cccagcatcc tacgcaattt
     1501 ttgggccgtg aaaggtagga agggttcaaa aacgatggct aggttggcgg ctatctgcag
     1561 gcagagattc agaatgctac ccacccgggg cagatctgtt ttggccagtt tccagggttc
     1621 ggtgtcggcc aggtatttat tcccgatgcg ggctagattc atggcttctt tttgggcatc
     1681 tctaaagcgg aatacatcga gcagggtttc gaccttgttt tggacgtcgg taaactcctc
     1741 cattgtttgc cggtcgtaat cgttaggttc gccccgagcc ggtactttcc cctgaaaata
     1801 cttatgagtg agtaccatag cccggttgac aaagttgccg aatacggcta ccaattcgtt
     1861 gttgttgcgg gcctgaaaat ctttccaggt aaaattattg tccttggttt cgggggcgtt
     1921 ggcggttaaa acataacgta atacatcctg tttacccggg aagtcttcga ggtattcgtg
     1981 caaccatacc gcccagtttt gcgaagtgga tattttgtcg tcttcgaggt ttagaaactc
     2041 attggccggc acgttgtcgg gcaaaataaa actgccttcg gctttgagca tggccggaaa
     2101 cacgatgcag tgaaacacga tgttgtcttt gcctatgaaa tggatcagtc tggtttcggg
     2161 gtctttccac catttttccc agctgtcggg caagagctct atggtatttg aaatataacc
     2221 gataggtgcg tcgaaccaca cgtagagcac tttgccttcg gcgccttcga ccggaacggg
     2281 aacgccccag tccaggtcgc ggctcaccgc cctggcttgc aggcccatgt cgagccacga
     2341 tttgcattgt ccgtagacat tgcttttcca ttctttatgc tcttcgagta tccattgttt
     2401 caaaaatgcc tcgtattggt ccaggggcag ataccagtgt ttggttgctt tcataacggg
     2461 tttgctcccg ctgatggccg aacgcggatg tatcagttcg ttggggctga gggagtttcc
     2521 gcaggattcg cattggtcgc cgtaggcgtt ttcgttgctg caatgcggac aggttccggt
     2581 gatatagcgg tctgccagaa actggcctgc ttcctcgtcg taatattgtt cactggtttt
     2641 ttcgacaaag ctgccctggc ggttgagttt gtcaaaaaag gcggaagcgg ttttgtgatg
     2701 gatggccgaa gtggtacgtg agtaaatatc gaaagaaata ccaaaatctt cgaaggattt
     2761 cttgatgatg tggtggtagc ggtctacaat agcctgaggg ctgactcctt ccttttttgc
     2821 tttgagtgta atgggcacac cgtgctcgtc ggagcccccg atgaacaaga cgtcttgtcc
     2881 tttgagtctg agataacgaa cataaatgtc tgccgggacg taaacgccgg ccaggtgtcc
     2941 gatgtgaacc ggcccgttgg catagggcag agcggtggtg acggtggtcc gtttgaattg
     3001 tcgggtcata agtatgaatg cttttaagcc gcaaaagtac aaatttatcg gggaattagc
     3061 agggcttttt tacgactctg ttttttctct ttgtttcccg gtttatttac cgtcttcgat
     3121 ggtgggtatc gggttcctca aagtatttcc gtgtgtgagg tttgtaaagc taagacataa
     3181 ttataaagcc gaagagtggg tagttttttt atgattgttc tgattctgac ctttgttggc
     3241 cttgttttcc ataaaaacac agagaatttt aaaaacaaga gaagaaagag aaaaatatcc
     3301 atgaagttta tattgcagat aatcagttat ataggctgtt gaaaacttgt tgaaaacttt
     3361 ttatctcatt ttatttaaaa aaagtgggtg aaaatgtagc aaagtgggtg aaaatgttta
     3421 tttttgtctg aaaaatttgc ctctatgggg taaaatagtc aaaatatagg ctgtaaaaag
     3481 acagaaacat gcgattttta ggaaatatcg aagctaaact ggatgccaaa aacagagtgt
     3541 ttgttcctgc cgcctttagg aaagtgctgg ataacgccgt ttttactaaa accgatgaag
     3601 gctcgcaaag tagaattttg tatttgcgca aagatgttta ccagaattgc atagtggttt
     3661 atccggctcc ggtatgggaa gaagaattga gcgaattgcg tgcccggctt aataaatgga
     3721 atcctcagga gcaggaactt taccggcagt ttatgctgga agccgaggcc gtagaaatgg
     3781 atcctaacgg acgtatattg atttccaaac gtctgttgtt aaaaaccggt atagaaaaaa
     3841 atgtacgttt tttaggcgtg gataatacca tggaaatctg ggcaaaagaa gctctcgagc
     3901 agccaagagt ggatccggag ctgtttaaaa cccggatgca ggaactaatg accaaaacca
     3961 aaatcccgga ataattacgc catggaaaca ccctaccata ttcctgtttt gttgagtgaa
     4021 agcgtagatg gcctggctct gaaaccggga ggcatctatg tagatgcaac ttttggggga
     4081 ggaggtcatt cgcgcgccat tctggcgcag cttaagggag gtcggctata tgcctttgac
     4141 caggatgaag aagcactgaa aaatttgccg gaagataaac gtttgcagtt tgttcacggc
     4201 aattttcgct ttttatccaa ttttatgcgt tattacggag aggaacaagt ggacggaatt
     4261 ctggcggatt tgggcgtatc gtctcatcat ttcgatcagg ccggcagggg attctctttt
     4321 cgttttgatg tcaaccttga tatgcggatg aatcaaagag ctactcttac ggctgcagat
     4381 attctggcag attattccaa agaagagctt tgtagagtat ttcgcgtgta cggagaattt
     4441 cgtcaggctg caaggatggc cggggctgtc gtgaaggcta gagaggaaca agctgtaaga
     4501 acggcgggtc agttgtcgga tttgttacgg cctttgttga gcaggcaagc cgaaaaaaaa
     4561 gacctggcca gagtttttca ggctttgcgt attgaactga atcaggaagt cggggctctc
     4621 gaggatttgt tgacacaatc cctggatatc ctgaaacccg gaggtcgact ggtggtaatc
     4681 agttatcatt cgctcgaaga ccgtttggtc aaaaatttta tgaggaccgg aaatctggag
     4741 ggtgaggtaa aaaaagattt ttacggcaac aatctcagtc cctttattct gatcaacaac
     4801 aaggtgctgg tgccttcgcc ggaagaacag gaactgaatc cgcgttcgcg cagtgcccgc
     4861 cttagaatag cagaaaagcg ttaagaaatg gaacaggaag aagaaaataa aaaaacgacc
     4921 aaacgtttta tttttggcga atttatagac ggaagctttc tggataagaa tttctttcgc
     4981 aaacacgcca aattgatatt ccttatagta gtgttgctct ttgtttatat aggcaatcat
     5041 tactcggtaa taatgaaact ggccgaaata aacaggttgc aacaagaact taacgatgta
     5101 aaatacgagg ctttgatgta cacctcggag tttatgcagg aaagccgcca atcgcatatt
     5161 aaatcgatgg tagaggaaaa aggcttggaa ttaaaggaat taaccgagcc tccctatacc
     5221 ttgaaagtaa aataaatgga gccgcttgca ggcttggaaa ggaaatggac gaacagacag
     5281 gtgaagacat aacaagaggt atacgtaaac gctatttgat catagtgatt ctgtgtttta
     5341 gtgtcatgct gtttggtatt atttttgcgg cttttcacat acaatttgcc gaaggggcgg
     5401 cctgggcaga atggaaaaac aaacaacagc gcgaaaacgt tgttgttccc gccggcagag
     5461 ggaatatttt ttcggacgag ggtttactga t
//
LOCUS       DAOSGL010000137         5483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13168_length_5483_cov_4.595431, whole genome shotgun sequence.
ACCESSION   DAOSGL010000137 DAOSGL010000000
VERSION     DAOSGL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13168_length_5483_cov_4.595431"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKY68_05290"
     CDS             complement(<1..445)
                     /locus_tag="PKY68_05290"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOH24486.1"
                     /translation="MTIRFTIHYHTYPGQELLFCGSQETTAWLRDEQAKPMTYRSGGY
                     WSLDLPVRQKGVFVYRYLVRENGRITRREWGRHQLTLTSLTEQFAEVLDTWQTEPAKS
                     AGTAIPVFSLRSDKDWGIGDFGDLFPMIDWLEQSGQKILQLLPVQD"
     gene            complement(580..849)
                     /gene="rpsO"
                     /locus_tag="PKY68_05295"
     CDS             complement(580..849)
                     /gene="rpsO"
                     /locus_tag="PKY68_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOH24487.1"
                     /translation="MYLTKEKKQEIFEKYGKSNTDTGSAESQIALFSYRISHLTEHLK
                     SNRKDYSTERSLKMLVGKRRRLLDYLKEKDIEKYRALIKDLGIRR"
     gene            944..1873
                     /locus_tag="PKY68_05300"
     CDS             944..1873
                     /locus_tag="PKY68_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOH24488.1"
                     /translation="MRKIIGIGESILDIVFKNRQPVSATPGGSVFNGLISLGRLGLPV
                     YFVSDIGQDQVGRFITDFMENNGLSSKYMNLHPDTNTAVALAFLDENNDANYTFYKDY
                     SVKGLQGNFPPINTDDIVIFGSYYALNPLLRKRVKAFLEKAVQKSALIYYDINFRKSH
                     SYQAKALNPVLIENYRYADIIRGSEDDFRALYQKQHAGGIYREKIAPYCENFIFTAAE
                     KNVEFYCPRFTASFPVKKIPTLSTVGAGDSFNAGILYGLMQSGICRKDLALLDQSDWE
                     KIIALGIDFSAKVCQSYENYIPRPESSNSGVNQ"
     gene            complement(1856..3085)
                     /locus_tag="PKY68_05305"
     CDS             complement(1856..3085)
                     /locus_tag="PKY68_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOH24489.1"
                     /translation="MNNDIYTLPNQIRVVRINCQADVSCCGFAIAAGSRDEEEKEQGL
                     AHFTEHLLFKGTHKRKAFHVINRLNAVGGELNASTGKEETFVYGLFLKNDFERALELL
                     CDMLFHSTFPQKEVEKEREVVLDEINSYEDSPSELIFDDFERCLFEHHPFGRNILGAP
                     ECLRAFGSSDLKAFARRCYTTDRLVFFSLSSLENKKTDALVQKYLAQIPDNSADSVLN
                     LRSSPQPVKAFKKHFSRNTHQAHVLYGNTAYSMFDEKRYALFLLNNLLGGPGMNSRLN
                     TLLREHSGLVYTVESGLSFYTDTGLCSIYFGSDPKNTDRCLSLIDKELKQLREQPLSS
                     SKLHYAKKQLFGQLSIASENKESRALNMGKSLLYYNKYQSLDDFMAKIEAVDAVQLCE
                     VASEVFNPSEMSLLVYS"
     gene            complement(3082..3705)
                     /locus_tag="PKY68_05310"
     CDS             complement(3082..3705)
                     /locus_tag="PKY68_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOH24490.1"
                     /translation="MSVVLLSTAYLAPVSYYVLLARAGKVVIEQHENYQKQSYRNRCL
                     IAAANGPMVLSIPVKKNLPGKNSIRDIQLSGHGNWQHMHWNSLVSAYASSPYFAFYRD
                     DIHPFYEKKYKYLFDFNEALREEICALLNLEPRLEYSKSYTTGPETEILDYRELIHPK
                     KQRLEELLPGPYVQVFDRKFGFLPDLSVLDLLFNTGPEACLFLMHKQ"
     gene            complement(3713..5164)
                     /gene="lepB"
                     /locus_tag="PKY68_05315"
     CDS             complement(3713..5164)
                     /gene="lepB"
                     /locus_tag="PKY68_05315"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH24491.1"
                     /translation="MSNTEKKCRFVATKRKWISFGLWTLVFILFVVWTGSLWWLLPIP
                     FAFDLFISRYIPWSFWKKSKNKTFRVIMDWVDAIIFALVAVYFVFLFFFQNYQIPSSS
                     LEKSLLVGDYLFVSKLSYGPRMPNTPLSFPLAQHTLPIVNSKSYFEKPQWPYKRIKGL
                     GKIQRKDIVVFNFPAGDTVALKMQNTDYYTICYSIARERGISMQEAARIVRTNPQTFG
                     EIVYRPVDRRENYVKRCVGLPGDSLQIISNQVYINGKILEDEPGTQYNYFVQTKGSYF
                     SEAQFRQWNISKEDRLLFPEQYSLPDLMGFEPDAQGNKLPVYHFPATKEVIEKIKKNP
                     MVNEVRIEPTQIGPYDLGGSIFTLSDEVSWTRDDFGPLWIPCKGATIELNPANVLLYG
                     RVICAYERHSLEERQGLYFIDGEPAEHYTFEMDYYWMMGDNRHNSADSRAWGFVPEDH
                     VVGKPMRVWLSLDKDRNWFQGKIRWKRFMKKAV"
     gene            complement(5176..>5483)
                     /locus_tag="PKY68_05320"
     CDS             complement(5176..>5483)
                     /locus_tag="PKY68_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOH24492.1"
                     /translation="QKLDAPSGTAISLAEDILKNLDRKKNWVNYESKESDELSIVSER
                     IGSVPGYHEVCYESEVDSLKIIHDAKNRAGLALGAVLAAEYTLGKKGFLGMHDLLGF"
BASE COUNT         1502 a         1256 c         1225 g         1500 t
ORIGIN      
        1 tgtcctgaac gggcaaaagc tgcaaaattt tttgtccgct ttgttccagc cagtcaatca
       61 tcgggaacag atctccgaaa tcacctatgc cccaatcctt gtccgaacga agtgaaaaga
      121 cggggatggc ggttccggcc gatttcgccg gttctgtctg ccaggtatcc agaacttctg
      181 caaactgctc tgtaagagaa gtaagcgtca attgatgacg cccccattcc cggcgggtga
      241 tgcgcccatt ttccctgaca aggtaacgat aaacaaacac ccctttttgc cgcacgggca
      301 aatccagcga ccaatatcct ccggaccggt aggtcatagg ctttgcctgc tcgtcgcgca
      361 accaggctgt tgtttcctga gagccgcaaa aaagcagctc ctgtcccgga taggtgtggt
      421 agtgaatagt gaaacggata gtcatgatcg aatggataat aaactgaacg gctttattta
      481 caggaatata ctgcggcgta atggtattaa aaaagaccgg ccccaaaagt cagaaacact
      541 ccaaaacttt caagaccggt ctttgttgtg gatcagcttt taacgtctga tacccaaatc
      601 tttgatcaac gcacgatatt tttcaatatc tttttccttg aggtagtcga gtaaacggcg
      661 acgtttgcct accaacatct taagggagcg ttcagtgcta taatctttac gatttgactt
      721 taaatgttcg gtcaaatgag aaatacggta tgaaaacaag gcgatctggg actcggcgga
      781 gccagtatcc gtgttagact tcccgtattt ttcgaagatt tcttgctttt tttctttcgt
      841 caaatacatt cttttgtaat ttaagtaatt agaaaatagt ggccgcaaag gtaatacttt
      901 tatttctatt ttcgagtatc tttgttcgtt gagttaaaaa attatgcgca aaataattgg
      961 tataggagaa agcatcctcg atatcgtatt caaaaacagg caacctgtgt cggccactcc
     1021 cggcggttca gtattcaacg ggctgatttc gctcggaagg ctgggtcttc cggtgtattt
     1081 tgtcagcgat ataggtcagg accaggtggg gaggttcatc accgatttca tggaaaacaa
     1141 cggcctttct tccaaatata tgaatctgca tcccgacacc aacaccgcag ttgccctggc
     1201 ctttctggac gaaaataacg acgctaatta tactttttac aaagattatt cagttaaggg
     1261 cctacagggc aattttcctc ccatcaatac cgacgatata gttattttcg gttcttatta
     1321 tgccctgaat cctttgctgc gcaaacgggt aaaagctttt ttggaaaaag cggtacaaaa
     1381 atcggctttg atttattacg acatcaattt cagaaaatcc cacagttatc aggccaaagc
     1441 cttgaatccc gtattgatag agaattaccg ctacgccgac attatcaggg gatcggaaga
     1501 cgacttcagg gcattgtacc aaaaacagca cgccggcggt atctatcgcg aaaaaattgc
     1561 cccctattgc gagaacttta tttttacggc agccgaaaaa aatgtggagt tctattgtcc
     1621 acgatttacg gcgagcttcc ccgtcaaaaa gatcccaacc ctcagcacag tgggcgccgg
     1681 cgactctttc aatgcaggta tactttacgg acttatgcaa tcgggtattt gccgcaaaga
     1741 cctggccttg ctcgaccaaa gtgactggga aaagattatc gctttgggca tagatttctc
     1801 ggcaaaggtc tgccaatcct acgaaaatta tattcccagg ccggagagtt caaattcagg
     1861 agtaaaccag taaagacatt tcagacggat taaacacttc ggatgccacc tcgcaaagct
     1921 gcacagcatc gacggcctcg attttggcca taaaatcgtc cagggactgg tatttgttgt
     1981 aataaagcag gcttttcccc atattcaaag ccctgctttc tttattttcg gaagctatgc
     2041 tcaactggcc aaagagctgt tttttggcgt aatgcaattt ggacgaactc aaaggctgct
     2101 ctcgcaattg tttcaattct ttatcgatga gcgacaggca acgatcggtg tttttaggat
     2161 cgctgccaaa atagatggaa cacaggccgg tatcggtgta aaagctgagc cccgattcta
     2221 cagtgtacac aaggccactg tgctcacgaa gtaaagtatt aagccggctg ttcatgcccg
     2281 ggccgccgag caggttgttc agcagaaaca aagcataacg tttttcgtcg aacatggaat
     2341 aggccgtatt gccgtacaaa acatgagcct gatgagtatt tctggaaaaa tgttttttga
     2401 aagcctttac cggttgagga ctgctgcgta aattaagaac cgaatcggcc gaattgtcgg
     2461 gaatttgggc caggtacttt tgcaccagag cgtccgtttt tttattttcc agggaagaca
     2521 gcgaaaaaaa gaccagacgg tcggtggtgt aacaacgccg ggcaaaagcc ttgagatcgg
     2581 aagacccgaa tgcccgcaga cattccggtg cacccagaat attccgccca aaagggtgat
     2641 gttcaaacag acaacgctca aaatcatcga aaatgagttc cgaaggagaa tcttcgtagc
     2701 tgttgatttc gtcgagcacc acttctcttt ctttttcgac ttctttttgg ggaaaagtgg
     2761 aatgaaaaag catgtcgcac aaaagttcca gcgccctttc gaagtcgttt ttgagaaaga
     2821 ggccgtaaac aaaggtttct tccttgccgg tgctggcatt caattctccg cccacggcat
     2881 ttaggcggtt gattacatga aaagctttac gtttatgcgt gcctttgaat aataaatgtt
     2941 ccgtaaaatg cgccagcccc tgttcttttt cttcttcgtc gcgagaacct gcggcaatgg
     3001 caaatccgca acaagaaaca tcggcctgac aatttatgcg aactacacgt atttgattgg
     3061 gcaaagtata gatgtcgttg ttcattgctt atgcattaaa aataaacagg cttccggacc
     3121 ggtgttgaac aaaagatcaa gcacggacaa atcgggtaaa aaaccgaatt tccggtcgaa
     3181 gacctgtaca taaggaccgg gcaacaattc ttcgagtctt tgttttttag ggtgtataag
     3241 ctccctgtaa tcgagtattt ctgtttccgg tccggttgta tagcttttgc tgtattcaag
     3301 cctgggttcc aaattaagta aagcacagat ttcttctctc aaagcctcgt taaaatcgaa
     3361 caaatacttg tatttctttt cgtaaaaagg atggatatcg tcccgataaa aagcaaaata
     3421 agggctggaa gcataagctg aaacaaggct gttccaatgc atatgttgcc aattcccgtg
     3481 cccggacagt tgtatgtcgc ggattgaatt tttgccgggt aggttttttt tcacgggaat
     3541 gctcaacacc atgggcccgt tggcggcagc aatcagacag cgattacggt agctttgctt
     3601 ttgatagttt tcgtgttgtt ctatcacgac cttcccggct cttgccagca aaacataata
     3661 agataccggc gccaggtagg ccgtcgataa caagactaca gacatgggct ttttaaacgg
     3721 cttttttcat aaaacgcttc cagcgtattt taccctgaaa ccaattacga tctttgtcga
     3781 gcgacaacca tacgcgcatg ggtttgccca ccacgtggtc ttcgggcaca aagccccagg
     3841 ctctggaatc ggcggaatta tggcggttgt cgcccatcat ccaataataa tccatttcga
     3901 aggtatagtg ctctgccggc tctccgtcaa taaaataaag accttggcgt tcctcgaggc
     3961 tgtgacgctc ataagcacat ataaccctgc cgtaaagcag cacattagca ggattcagct
     4021 ctattgtcgc acctttgcag ggtatccaaa gaggaccgaa atcatcgcgg gtccatgaaa
     4081 cttcatcgct taaagtaaaa atcgacccgc ccagatcgta aggaccaatc tgggtaggtt
     4141 ctatacgaac ctcattcacc atgggattct tttttatttt ttcaatcacc tctttggtgg
     4201 ccggaaaatg atacaccggt agtttgttgc cttgagcatc gggctcaaaa cccatcaaat
     4261 cgggcaaaga atactgttca ggaaagagca aacgatcttc tttgctgatg ttccattgtc
     4321 tgaactgagc ttcactgaaa taggagccct tggtttgcac aaagtaattg tattgggtgc
     4381 cgggctcgtc ttccaggatt tttccattga tatacacctg attgctgatg atttgcaaac
     4441 tgtctccggg caggccgacg catcgtttaa cgtaattttc acgccggtcg acaggacgat
     4501 acactatttc gccgaaagtt tgaggatttg tgcggacaat gcgggctgcc tcttgcatac
     4561 ttatacctct ttcgcgggca attgaatagc aaatggtata ataatcagtg ttctgcattt
     4621 tcagggcaac cgtgtcaccg gcggggaaat taaataccac tatatccttg cgttgaattt
     4681 tacccaggcc ttttatacgt ttgtaaggcc attggggctt ttcaaaatag gatttgctgt
     4741 ttactatagg cagagtatgt tgggccaaag gaaacgataa gggggtattg ggcatgcgcg
     4801 gtccgtagct gagcttgctg acaaataaat aatcgcccac caacagcgat ttttcaagcg
     4861 aagaactggg aatctgataa ttttgaaaaa agaacaaaaa gacaaaatac accgccacta
     4921 aggcaaatat aatggcatct acccagtcca ttattaccct gaaggtctta ttcttggatt
     4981 ttttccagaa agaccaggga atatagcggc tgataaacaa atcaaaagca aaaggaatgg
     5041 gcaaaagcca ccacaaactg cccgtccata ccacaaaaag gataaagacc agggtccaca
     5101 agccaaaact tatccacttg cgtttggtag cgacaaatcg acattttttt tctgtattgc
     5161 tcatattttt tatgattaga atccgagtaa atcgtgcatg cccaaaaagc cttttttgcc
     5221 cagggtgtat tcggcagcca atactgcccc cagggccaat ccggccctat tcttggcatc
     5281 gtgtataatt ttgaggctgt ctacctccga ctcgtagcaa acttcgtgat aaccgggcac
     5341 actgcctatg cgttcggaaa ctatactcaa ctcgtcggac tccttacttt cgtaatttac
     5401 ccagtttttt ttccggtcaa ggtttttcag gatatcttct gccaggctta tggctgttcc
     5461 gctgggagca tccagtttct gga
//
LOCUS       DAOSGL010000138         5477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13186_length_5477_cov_5.387311, whole genome shotgun sequence.
ACCESSION   DAOSGL010000138 DAOSGL010000000
VERSION     DAOSGL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13186_length_5477_cov_5.387311"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2582)
                     /gene="infB"
                     /locus_tag="PKY68_05325"
     CDS             complement(<1..2582)
                     /gene="infB"
                     /locus_tag="PKY68_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOH24493.1"
                     /translation="MSSVRLNKITRELNVGIGTLVEFLQKKGFEVENNPNAKISGEAY
                     MLLVEEFGDGQELLIPKKEKPKIRETEQVTPEPVKQEAPEEPVVSTRKQQFKTLGKID
                     LDAVAKPKATGPKKEEKEEILPKEEKPAKTVEEVITAVASPASTEPEAAEKIKKEESP
                     VQESAKKAPAAEAPVSRTPAADMAEKEEKPTPQKETVRKEEKAIVEEKEEIPETIPPK
                     KTEEQEDAQEDEKSEEPEIFTLGKRTIKPNIKVVTTIDLDALNQNTRPKRKTKQERLK
                     EQEDKKTQEQALREERKKAETLKGRTEGKTEVSDSKKKRRSRIKDVKIKVDGEFGAPV
                     SRGDRTKGRLKKPVLKTEVSEEDVSKQIKETLARLTSRPKKGAKYRREKREAASQRMQ
                     ELEQKEMREKSVLKITEFVTVNDLATMMDVPVVDVISTCIAIGQMVSINQRLDAETIN
                     FVADEFGFKTEYVSAEVLEAIQEEEDDEEDLVPRPPIITVMGHVDHGKTSLLDYIRNS
                     NVIAGEAGGITQHIGAYNVTLKDGRKMTFLDTPGHEAFTAMRARGAKITDIAIIIVAA
                     DDNVMPQTVEAINHASAAGVPMVFAINKIDKPAADPEKIKQELANMNYLVEEWGGKYQ
                     SQDISAKKGINVKELLEKVLLEAEMLELKANPSRRAVGSIIESTLEKGRGYVATVLVE
                     NGTLEVGDNIVAGVNHGRIKAMFNERGQRVESAGPAEPVLILGLNGAPQAGDNFNVLD
                     SEQEAREIATKREQLQREQGLRTSKILTLAEIGRRIAVGNFKELNLIVKGDVDGSIEA
                     LSDSLIGLSTEQIQVNVIHKAVGQISESDVTLASASDAIIVGFQVRPSMAARKMAERE
                     GVDIR"
     gene            complement(2597..3868)
                     /gene="nusA"
                     /locus_tag="PKY68_05330"
     CDS             complement(2597..3868)
                     /gene="nusA"
                     /locus_tag="PKY68_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOH24494.1"
                     /translation="MAKTEERINLVEIFSEFKESKNIDRSTMINVIVESFRGVIAKLF
                     GSDEHYDVIINPDSGDFEIWHNREIVEDDQVEDPEHQISLTEARKIESDYEVGEEVTD
                     QVHFSAFGRRAILNLRQTLASKIMELQKESLYNRFIEQVGQLISAEVYQIWKKEILLL
                     DDENNELILPKSEQIPSDFYRKGETVRAVIERVENKTNNPKAVDPKIILSRTSPVFLE
                     RLFELEVPEIHDGLITIKRVARIPGERAKIAVESYDERIDPVGACVGVKGSRIHGIVR
                     ELRNENIDVINYTSNTSLFIQRALSPAKISSIRLDEVNKKAEVWLKPEEVSLAIGKGG
                     MNIKLASMLTNYTIDVFRETDQELEEDDIYLDEFKDEIDEWVIEALKSIGCDTARNVL
                     ALPRETLVEKADLEESTVDEVLRILHAEFED"
     gene            complement(3916..4383)
                     /gene="rimP"
                     /locus_tag="PKY68_05335"
     CDS             complement(3916..4383)
                     /gene="rimP"
                     /locus_tag="PKY68_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOH24495.1"
                     /translation="MISKEIINQYIEEWINNKPFFLVDLNIGPDFSIVIEIESIQGNV
                     SIGDCAELSRHIESRLSNEDLDFALKVSSAGIGQPFKVFRQYQKHINKEVEVRLKNGQ
                     KFSGTLLSCDEDKFTVNVMRRIKPEGAKRPKIVEQQECYLQAETASVCSKIMF"
     gene            4549..5205
                     /locus_tag="PKY68_05340"
     CDS             4549..5205
                     /locus_tag="PKY68_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH24496.1"
                     /translation="MKSVFYPLLIFWSCFTLFCSCSTISNIVKKEFAERNPEDILQNT
                     AAEYIVNSSIEGEAGRWIASQMEILKIMLSEQIGDSVMIRQPEEGLLLVFDSDRLFKQ
                     GLQYVNDSSIETLEVLATSLQDFPNTRLYILVHTDNSSSEFIALSLSDKRAKNIKRIL
                     ASKGMKRSHITAEGRGFMVPVISNESPEGRKINRRVEMAVTANHKMIRQAIKSTKTKR
                     "
     gene            5255..>5477
                     /locus_tag="PKY68_05345"
     CDS             5255..>5477
                     /locus_tag="PKY68_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024100.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOH24497.1"
                     /translation="MSKKAMLMILDGWGIGSRGKEDVIYNTPTPYWDYLIKNYPNSQL
                     QASGENVGLPDGQMGNSEVGHLNIGAGRVV"
BASE COUNT         1293 a         1303 c         1137 g         1744 t
ORIGIN      
        1 cgaatgtcca cgccttcacg ttcggccatc ttgcgggcgg ccatagaggg gcgtacctga
       61 aaaccgacaa taatggcatc ggaagcagaa gccagtgtta catccgattc ggaaatctga
      121 ccgaccgcct tgtgtatcac attcacctga atttgctctg tgctcaaacc tatcagagaa
      181 tccgacaaag cttcaataga tccgtccaca tctcctttta caatgagatt cagttctttg
      241 aaattgccaa cggctatacg gcggccgatt tcggccagtg tcagaatttt actggtgcgc
      301 aacccttgtt ctctttgcaa ttgctctctt ttggtggcaa tctctctggc ttcctgttcg
      361 gagtcgagta cgttgaaatt gtcgcctgcc tgaggagccc cgttcagtcc aaggatcaat
      421 acaggttcgg cgggccctgc cgattccacg cgttgccctc gttcattgaa catagctttt
      481 atgcgtccgt gattaacgcc ggcaacgatg ttgtctccga cttccaacgt gccgttttcc
      541 accaatactg tcgccacgta gccacgcccc ttttcgagtg tagactctat gatggacccc
      601 acagcacgtc ttgagggatt ggccttgagt tccaacattt cagcctcgag caaaaccttt
      661 tcgagcaatt ccttcacatt gatgcctttt ttggctgaaa tatcttgtga ttggtatttt
      721 ccgccccatt cttcgaccag gtaattcata ttagccagtt cctgcttaat tttttcgggg
      781 tcggctgccg gtttgtctat tttgttaata gcaaatacca tggggacgcc ggctgctgaa
      841 gcgtggttaa tggcctcaac tgtttggggc ataacgttgt cgtcggctgc cacaataata
      901 atggcaatat ccgttatttt ggctccgcgg gcgcgcatgg ccgtaaaagc ctcgtggccc
      961 ggagtgtcca gaaaagtcat tttccgtccg tctttcagag taacattgta tgctccgatg
     1021 tgctgagtta tacctccagc ctcacctgca atcacattag aatttcggat gtagtcgagc
     1081 agagaagttt taccgtggtc cacgtgtccc attaccgtga taatgggagg acgaggcacc
     1141 aaatcttctt cgtcgtcctc ttcttcctga atggcctcca atacttcagc actgacatac
     1201 tctgttttaa agccgaattc gtcggctaca aaattaatgg tttctgcgtc caggcgttga
     1261 ttgatcgaca ccatctgacc tatggcgata caagtggaaa ttacgtcgac aaccggcaca
     1321 tccatcatgg tagccaggtc gttaacggta acaaactcgg ttattttaag cacgctcttt
     1381 tcccgcattt ctttttgttc gagttcttgc atacgttgag atgccgcctc tcgtttttct
     1441 ctgcggtatt ttgctccttt tttgggcctg ctggtcaggc gggcaagtgt ttctttaatt
     1501 tgtttggaaa catcctcttc gctgacttct gttttaagaa cgggtttctt gaggcggcct
     1561 ttggtcctgt cgcccctgga gaccggagcg ccgaattctc catcgacctt tatcttgaca
     1621 tccttgatgc gactgcgcct tttctttttg gaatctgaaa cttcggtttt cccctcggtt
     1681 ctccctttaa gagtttcggc ttttttcctt tcttcgcgca aggcctgttc ttgtgttttt
     1741 ttgtcttcct gttccttgag tctttcttgt ttggttttcc ttttgggcct ggtattttga
     1801 ttcaacgcat ccagatcaat ggtggtaacc accttgatgt tgggtttaat agtccttttc
     1861 cccagggtga atatttcggg ctcttcagat ttttcatcct cttgggcgtc ttcctgttct
     1921 tcggtttttt taggaggtat tgtttcagga atttcttctt tttcttcgac aattgccttc
     1981 tcttccttgc ggacggtttc cttctgaggg gtgggttttt cctctttttc agccatatcg
     2041 gctgcaggtg ttcggctcac aggagcttct gctgccgggg ctttttttgc agattcctga
     2101 acgggggatt cttctttttt tattttttcg gctgcttcag gctcggttga tgccgggctt
     2161 gcgacagcag taataacttc ctcaacagtt tttgcgggtt tttcttcctt aggaaggatt
     2221 tcttcctttt cctctttttt cggtcctgtt gctttgggtt tagcaacagc atccaggtct
     2281 attttaccca gagtcttgaa ttgttgtttg cgagtgctta cgacaggttc ttcgggagcc
     2341 tcttgcttaa caggctcagg agttacctgt tccgtttctc tgatcttggg tttctccttt
     2401 ttgggaatta aaagctcctg cccatcgcca aattcttcga cgagaagcat ataggcctct
     2461 ccgctgattt tggcgttggg gttattttca acctcaaatc cctttttttg taaaaattcc
     2521 accaaagtgc cgatacccac attcagctct ctggttattt tattcaatcg aacactcgac
     2581 ataagcaaat aatttattaa tcttcaaact ctgcatgaag tatacgcaag acttcatcca
     2641 cagtactttc ctccaaatcg gctttttcaa ctaaagtttc tcttggcaaa gccaacacat
     2701 tccgagcagt atcgcaacct atggatttta gggcttcaat aacccattca tctatttcgt
     2761 ctttgaattc atccaggtaa atatcatcct cttccagttc ctggtcggtt tctctgaaaa
     2821 catctatggt ataattcgtc aacatgctgg caagctttat gttcatgcct cccttgccaa
     2881 ttgccaatga aacttcttcg ggcttgagcc atacctcagc ttttttgttg acttcgtcca
     2941 gcctgataga agaaattttg gctggactca aggccctttg aataaacaaa ctggtgtttg
     3001 aggtatagtt gatgacgtca atattctcgt tgcgcaattc acgtactatg ccatggattc
     3061 tggagccttt cacacccaca caggctccga ccggatcaat acgttcatcg taagactcca
     3121 cggcgatttt tgctctttct cccggaatgc gggctaccct tttgatggta atgagcccgt
     3181 catggatttc gggaacttcc aattcaaaaa gcctctcaag aaaaacagga gaggtacgcg
     3241 acaaaattat cttggggtca acagctttgg gattgttagt cttgttttca acgcgctcta
     3301 tgacggcacg aaccgtttct cccttacggt aaaaatcgct gggtatctgt tcactcttag
     3361 gtagaatcag ttcattattt tcatcgtcca aaagcagaat ttcttttttc caaatctggt
     3421 agacttcggc gcttatcaat tgtcccactt gttcaatgaa acggttgtag aggctttcct
     3481 tttgtaattc catgattttt gaagccaggg tttgccgcaa attcaaaatg gcacgacgtc
     3541 cgaaggcact gaaatgtacc tggtcggtaa cttcctcacc tacttcgtaa tcgctttcta
     3601 ttttacgggc ttctgtcagg ctgatttggt gttcgggatc ctcgacctgg tcgtcttcta
     3661 ctatttcgcg attgtgccaa atttcaaagt ctccgctgtc cgggttgata ataacatcgt
     3721 aatgttcgtc agagccaaac agttttgcta tgactccacg aaaagattct acaatcacat
     3781 ttatcattgt agaacggtct atgtttttag actctttgaa ttccgaaaaa atttcgacca
     3841 gattgattcg ttcttctgtt tttgccattg ttatcgacat tgtgttgaaa tatctaatta
     3901 attctctcgt atttatcaga acattatttt ggaacatacg gaggccgttt ccgcttgcaa
     3961 atagcactct tgctgctcaa caattttggg ccttttggca ccctcgggct ttattcttcg
     4021 cataacgttg acagtaaatt tgtcctcgtc acagcttaat aacgtacctg aaaacttttg
     4081 tccgtttttt agacgaacct caacctcctt gtttatgtgc ttttgatatt gcctaaatac
     4141 tttaaagggt tggcctatgc ctgccgaaga aactttcagg gcaaaatcca gatcctcatt
     4201 gctcagacgg gactctatat gcctgctcaa ctctgcgcaa tcgcctatac ttacattgcc
     4261 ttgaatagat tcaatttcta tgacaatact aaaatcgggg ccgatattga ggtcaaccaa
     4321 aaaaaagggc ttgttattaa tccactcttc aatgtactga ttgataatct ctttactaat
     4381 catccttttg tgttaagtaa aaaatgaggg gaacttttgt ccccctcgcc tgtatcctaa
     4441 cggccgcaaa attacacaat atcaagtgat ttgcaaaaaa aacgcagatt attttaatct
     4501 ggaggctaaa aaatatactt ttgcgcggac tttgatccga aatattgtat gaaaagcgtt
     4561 ttttatcccc ttttgatatt ttggagctgt tttacattgt tctgctcttg ctctaccatt
     4621 tcgaatattg taaaaaaaga atttgctgaa aggaatcccg aagacatcct ccaaaacacc
     4681 gctgccgaat acattgtaaa ttcgagcatc gagggagaag ccggccggtg gattgcctca
     4741 caaatggaga tcctaaaaat catgctaagc gaacaaatag gcgattctgt gatgattcgc
     4801 caacccgaag aaggcctttt gctggttttt gactccgacc ggctttttaa acaaggcctt
     4861 cagtatgtaa atgattcttc catcgaaact ttagaagtac ttgcaacatc cctgcaagat
     4921 tttccaaaca ccagattgta tatcctggtc cataccgaca acagcagttc agaattcata
     4981 gcgctttcct tatcggacaa aagagccaaa aacataaaaa gaattctggc gtcaaagggc
     5041 atgaaacgca gtcatatcac agccgaagga cgagggttta tggtgcccgt tatttcgaac
     5101 gaaagcccgg aaggccgtaa aataaaccgc cgggtagaaa tggctgtaac ggccaaccat
     5161 aaaatgattc gccaagctat aaaaagcact aaaacaaaaa gataattcag ccgaattgct
     5221 tacctttgcg cccaatattt ttacatgatt atttatgagc aagaaagcaa tgctgatgat
     5281 tctcgacggt tggggtatag gcagccgtgg gaaagaagac gttatataca acacccctac
     5341 tccttactgg gactatctga taaaaaacta tccgaactcg caacttcagg catcgggcga
     5401 aaacgtgggt ttacccgacg gtcaaatggg caattccgaa gtgggacatc tcaacatcgg
     5461 agccggcaga gtggttt
//
LOCUS       DAOSGL010000139         5395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13453_length_5395_cov_4.486142, whole genome shotgun sequence.
ACCESSION   DAOSGL010000139 DAOSGL010000000
VERSION     DAOSGL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13453_length_5395_cov_4.486142"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKY68_05350"
     CDS             <1..541
                     /locus_tag="PKY68_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOH24498.1"
                     /translation="IEVGRLLLTKDSVFLMNRLRKSYAVAALSDQNIGNDQAVYALKV
                     LTSVLSNQFFVPGNKQISAKDFDLQETDRELILSCSHQNEKANFYINKAGEYYRADLE
                     SAMLNFPLKVDYSLFQATTFGSFPSRVVLSFQQGNDNIEFRVDYLNPVYNTGLSLDFP
                     LPGNYKQTSVESFLNQLQF"
     gene            580..1767
                     /locus_tag="PKY68_05355"
     CDS             580..1767
                     /locus_tag="PKY68_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOH24499.1"
                     /translation="MNFAALQAQQSIEDVRKERLAVLERIKAANESLSANQKSSKALL
                     YQYTILSDQIKSTEQYIDRLNTEMNRLSRSLRTLQNEYNELKRQLSVKQQQYAQLIKQ
                     LHTRLKIDDKLMFIFSAEDLGQSYRRIRYLNEYTKFQRKQAEEIRQKQVQLEKKRIDL
                     EVAYQQQKELLDEQQAERDRLSKEREKQRNLLNTLKKKESTLNAELKKDRELAARLDK
                     QIEEIIAEETRRASLEKERSRPESEGGYAMTIDEKILSDDFGNNKGKLPFPLNEPGTI
                     IVHFGQQKFQELKYVQTNSKGIDIQTRAGASARAVFKGTVSKIFMVPGMNASVIVRHG
                     KYLTVYSNLGEVRVKLGDKVKTGELLGKVFVDEQQGNQTILHFQMFQDRERLNPEVWI
                     KKF"
     gene            complement(1884..2936)
                     /locus_tag="PKY68_05360"
     CDS             complement(1884..2936)
                     /locus_tag="PKY68_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOH24500.1"
                     /translation="MDYKSSIDLLKELIAIPSFSKEEKARADFLCRYLRELGFEPQRV
                     GNNLWCTAENIDDSKPNLLLNSHIDTVRPSDSWTRRPFVPQIEGDRLYGLGSNDAGAA
                     LVCLIRTFWRLSRKPQPYNLMLGLSAEEEITGAQGIELLMPALPPVDLAIVGEPTGMQ
                     AAVAEKGLLVLDCIAPGKSGHAAHDSADNAIYNAIEDINWFRKTTLPRSSDLLGPVKM
                     TVTVIQAGEKHNVVPDNCRFTVDIRVNEKYNNQELYDYICTQVKCSVTARSFRLGSSS
                     ISPAHPIVHACKAQGLTLYGSPTLSDQARMHCPSIKIGPGQSLRSHTADEFVLLSEIR
                     EGAEVYFRLLDGLKIR"
     gene            3061..4881
                     /locus_tag="PKY68_05365"
     CDS             3061..4881
                     /locus_tag="PKY68_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOH24501.1"
                     /translation="MNIYHPSRLVPEYAARCGDRIAMRYRDYTDGRWKGISWKELNDR
                     IDHLCAALLNFGIKEQEKVGIFSQNMPECIVSEFSIFSLRAVSVPMYATSTTSQIEYI
                     VADAGIRMIFVGEQYQYDRAFEAISHGECGLKQIVIFDTNVRLQKNDDQSIYFKDFVH
                     KGMLLNLQDSVEAKRSKCRDEDLANILYTSGTTGEPKGVQLLHSNYQEIIRVMDIKLL
                     HLFDKNEVSMCFLPLTHIFEKAWSLYSLSRLIRVDINLKPLDIQQSLKEVRPTIMCNV
                     PRFWEKVYSGIMEKIEAMPALAQKWVFHAVKLGKRYNLDYVRLDLKPPFWLSLRYNLY
                     RVSLFTILKKAVGLENGKLFPVSGAKLSDDILIFMRSVGIPICYGYGLTETTATVASF
                     DEKHFSLGSVGSFIDKLQVRIDDNGEILLKGETITPGYYNKPEINAKAFTEDGFFRTG
                     DMGYINKEGHLVLTERLKDLFKTSNGKYIAPQSIEAVLCNDKYIDMVAVIGDQRKFVS
                     ALIVPAYEELKSLAEKLGIPDLDADKLEDVLKDERIYAFFENRIRMLQKNMAGYEQIR
                     RFVLLPKPFSVADGELTNTLKMKRNVIADHYKDEIEKMYL"
     gene            4881..5395
                     /locus_tag="PKY68_05370"
                     /pseudo
     CDS             4881..5395
                     /locus_tag="PKY68_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445733.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
BASE COUNT         1611 a         1285 c         1255 g         1244 t
ORIGIN      
        1 tatagaggtg ggccgcttgc tgcttaccaa agacagtgtg ttccttatga atcgactccg
       61 gaagtcttat gccgttgctg cactttccga tcaaaacata ggaaacgatc aggcagttta
      121 tgctttaaag gttttgacct ccgtactttc aaatcaattc ttcgtacccg gcaacaaaca
      181 aatcagtgct aaggattttg acttgcagga aaccgaccgg gaattgattt tgagttgttc
      241 tcaccaaaac gaaaaggcaa acttttacat caataaagcc ggagaatatt atcgggctga
      301 cttggagagc gctatgctga attttcccct caaagtggat tacagccttt ttcaggctac
      361 cactttcggc tctttccctt ccagggtagt cctgagcttt caacaaggca acgacaatat
      421 agaattcagg gtagattatc tcaacccggt ctacaacacc gggctcagct tggattttcc
      481 gctaccgggc aattacaaac aaaccagtgt ggaatcgttt ttaaatcaac ttcagtttta
      541 acgatgcaac gaatacattg ttttctcatc ctgttaatct tgaattttgc cgcactccag
      601 gcccagcaaa gcatagaaga tgtccggaaa gaaaggttgg ccgtgctcga gcgtatcaaa
      661 gcagccaacg aaagcttgag cgccaaccaa aagtcgagca aagccctgct ttatcaatac
      721 accatcctga gcgatcagat caaatcgacc gaacaataca tcgaccgcct gaacaccgaa
      781 atgaacaggt tgtcccgttc cttgcgcact cttcaaaacg aatacaatga actcaaacgg
      841 caactgtcgg tcaagcaaca acaatacgct caattaataa aacaacttca tacccggctc
      901 aaaatagacg ataagctgat gtttattttt tcggccgaag acctcggcca aagctatcgc
      961 cgtattcgct acctgaatga atacaccaag tttcagcgta agcaggccga agaaatacgt
     1021 cagaaacaag tacaactcga aaaaaaacgc atcgatctgg aggtggctta tcagcaacaa
     1081 aaagagctgc tcgatgaaca gcaagccgag cgcgaccgcc tgagcaagga aagagaaaaa
     1141 cagcgcaact tgctgaatac cctcaaaaaa aaggaatcga cactgaacgc cgagctcaaa
     1201 aaagaccgcg aactggccgc ccgactcgat aaacaaattg aggaaatcat tgctgaagaa
     1261 actcgcagag ccagcctcga aaaagaacgc agtcgacccg aaagcgaagg cggctatgcc
     1321 atgaccatag acgaaaaaat tttatcagac gactttggca acaacaaagg caaactcccc
     1381 ttccccctga acgaaccggg aaccatcatc gtgcactttg gccaacagaa atttcaggaa
     1441 ctcaaatacg tgcaaaccaa cagcaagggc atcgatatcc aaacccgggc aggagcttcg
     1501 gccagagccg tttttaaggg taccgtcagt aaaatcttta tggtaccggg tatgaacgct
     1561 tccgtcattg tgcgccacgg caaatacctt acggtctatt ccaacctcgg agaggtcaga
     1621 gtaaaattgg gagacaaggt aaaaacgggc gaattgctgg gcaaagtctt tgtcgacgag
     1681 caacagggca accaaaccat cctgcatttt caaatgtttc aggataggga gcggctgaat
     1741 ccggaggttt ggatcaagaa attttgagcc accgcccgaa gtttactgta gtcttccgtt
     1801 tgagactttt cgctttatca cctgatcttc aaccctcatt cgggaaagac agctctgttg
     1861 cgttgcattg actaatcgct ttatcaccta atcttcaacc cgtcgagcaa acgaaaataa
     1921 acctctgccc cttcccgaat ttcggataac aaaacaaatt catcggcggt gtgtgagcgc
     1981 aacgactggc cggggccgat ttttatactg ggacaatgca ttctggcctg atccgagagc
     2041 gttggggaac catacaaagt caggccctga gccttgcagg catgaacgat gggatgagcg
     2101 ggggaaatgg aagaagaacc cagacggaaa gaacgggcag tcaccgagca cttaacctga
     2161 gtgcaaatat aatcgtacaa ttcctgattg ttgtatttct cgtttacccg gatatccacg
     2221 gtaaaacggc agttgtcggg gacaacgtta tgcttttcgc cggcctgaat cacggtaacg
     2281 gtcattttaa cgggtccgag taaatcggac gaccggggaa gagtggtttt ccgaaaccag
     2341 ttgatgtctt cgatggcgtt gtagatggca ttgtcggcac tgtcgtgagc agcatgtccc
     2401 gacttcccgg gggcgatgca atccaacacc aacagcccct tttcggcaac ggccgcctgc
     2461 ataccggtag gttcacccac aatggccaga tctacaggcg gcagggccgg cataagcaat
     2521 tctatgccct gagctccggt gatttcctcc tcggccgaca aacccagcat cagattgtaa
     2581 ggttggggct tgcgactcag cctccaaaaa gtccggatca ggcaaaccag cgcagccccc
     2641 gcatcgttgc tgcccaggcc gtaaagacgg tctccctcga tttgaggtac aaagggccgg
     2701 cgggtccagg aatccgaagg gcgcaccgta tcgatatggg aattgagcaa caagttcggt
     2761 ttgctgtcgt ctatgttttc tgccgtacac caaagattgt tgccgactcg ttggggctca
     2821 aagccaagct cccgaaggta acggcataaa aaatcggccc tggccttttc ttctttgctg
     2881 aaagagggta tggctataag ctctttcaat aaatctatgc tgcttttgta gtccataatt
     2941 attgataatc aacatgcaaa attagctaag aatatcttta aacaatacaa atcttggagc
     3001 ttttgaaaaa aaatataaat tataagcgta cttttgcatg tcaaatcaat tctatatcaa
     3061 atgaatattt atcatccttc cagactggtg cctgaatacg cggccaggtg tggtgaccgg
     3121 attgccatgc gctaccggga ttataccgac ggtcgctgga aaggtatatc gtggaaagaa
     3181 ctgaacgatc gtatcgacca tctgtgtgcc gccttgttga attttggcat caaagaacag
     3241 gaaaaggtgg gtatcttttc tcaaaatatg cccgaatgta tcgttagtga gttttccatt
     3301 ttcagcttaa gggcagtaag cgtacctatg tacgccacca gtactacaag ccagattgaa
     3361 tacatcgttg cagacgcggg tatccggatg atttttgtag gtgaacaata tcaatacgac
     3421 cgggctttcg aggccattag tcacggagaa tgcggactga aacaaatcgt tatttttgat
     3481 accaatgtgc gcttacagaa aaacgatgat caatcgattt atttcaaaga ctttgtacac
     3541 aaaggaatgc tgcttaacct acaggattcg gtagaagcca agcgcagtaa atgtcgtgac
     3601 gaagacctgg ccaacattct ttatacttcg ggcaccaccg gtgagcccaa aggcgtgcaa
     3661 ctcctacaca gcaattatca ggaaatcata agagtgatgg atatcaaact cttgcatctc
     3721 ttcgataaaa acgaggtttc gatgtgtttc ctgcccctga cccacatatt cgagaaagcc
     3781 tggagcctgt atagtctttc gaggctgatc cgggtggata tcaatcttaa gccgctcgat
     3841 attcagcaga gcctcaaaga agttcgtccc actatcatgt gcaacgttcc ccggttttgg
     3901 gaaaaagtgt acagcggcat tatggagaaa atcgaggcta tgcctgccct ggctcaaaaa
     3961 tgggttttcc atgccgtcaa actgggtaaa agatacaacc tcgattacgt gcgtctggat
     4021 cttaaacctc ctttttggct gagtctgcgc tataaccttt acagagtaag cttgtttacc
     4081 attctcaaga aggcggtagg cctggaaaac ggcaagcttt ttcccgtttc gggagccaaa
     4141 ctgtcggacg acattctgat ttttatgcgc tcggtgggca tacccatctg ttatggttat
     4201 ggtctgaccg agaccacagc tacggtggcc agctttgacg aaaagcattt cagtctcggc
     4261 agtgtgggca gttttatcga taagttgcaa gtgcgcattg acgacaacgg agaaatcctg
     4321 ctcaaaggcg aaaccatcac tccagggtat tacaacaaac ccgaaatcaa tgccaaagcc
     4381 tttaccgaag atggtttttt ccgcaccggc gatatgggtt acatcaacaa agaaggacat
     4441 ctggtgctga ccgagcgcct aaaggatttg tttaaaactt ccaacggcaa atacatagct
     4501 ccccaaagta tagaagcggt gctatgcaac gacaaataca tcgacatggt tgccgtcatc
     4561 ggggatcagc gaaaatttgt cagcgccctg attgtgcccg cttatgagga actgaaaagt
     4621 ttggcggaaa aactgggcat acccgatctt gatgccgata agctcgaaga tgtgcttaaa
     4681 gacgagcgga tatatgcctt ttttgagaac agaatccgca tgttgcaaaa aaacatggcc
     4741 ggttacgaac aaatccgccg ttttgtttta ttacccaagc ctttcagtgt agccgacggc
     4801 gaattgacca acacgcttaa gatgaaacgc aacgtgattg ccgatcatta caaagatgaa
     4861 atagaaaaaa tgtatttata atgattacta cagaccagat caaagagatc caggagcgcg
     4921 agcacgcgct gaggaggtat ctttgacatc gaaaacaaaa ttgacgcttt aaaagaagaa
     4981 gaactcaaga cccaggctcc cggtttctgg gaaaagcaaa aagaagccga ggagcaaatg
     5041 cgcaaaatca agcaactcaa aggctggata gaagattacg aagaagtcca aactgccgta
     5101 caggagctga cgctggctta tgatttttac aaagaagagg ccctgagcga aaaggaaatg
     5161 gatgagcaat acgatcgcac cctgaatctg ctcgaaaacc tggaaatgaa aaatatgctc
     5221 cgccacgaag aagacaaact cggggccgta ctcaaaataa atgccggagc gggcggcacc
     5281 gaaagccagg actgggccga aatgctctac cgcatgtatc agcgctattg cgataaacaa
     5341 ggctacaaaa ccaacatcac caactggcaa gacggagacg acgccggcat caaaa
//
LOCUS       DAOSGL010000140         5373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13529_length_5373_cov_4.378150, whole genome shotgun sequence.
ACCESSION   DAOSGL010000140 DAOSGL010000000
VERSION     DAOSGL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13529_length_5373_cov_4.378150"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PKY68_05375"
     CDS             <1..774
                     /locus_tag="PKY68_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24502.1"
                     /translation="DDFDYNTVEGLYYLKDGVPDISPNITGVMHGIATPTGQNAYPGM
                     AKYSDTDGSGMVDGEDFTIIGDMNAKHTGGFHITAQYKQFDLGLYFNWSYGNQVYNVN
                     RMASLMGYKESGVYQNKLVVLASSYKIYDIVNGQLVRYNTPEELAALNKNAEYPLCYN
                     ESGVVSSLGIEDGSYLRLNTLNFGYTLPKSVSTKLKINNMRLFFTAYNLLTLTKYQGL
                     DPEVNTNERVNHAVYPTPGLDWGTYPRPRSLVVGANISF"
     gene            794..2842
                     /locus_tag="PKY68_05380"
     CDS             794..2842
                     /locus_tag="PKY68_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24503.1"
                     /translation="MKKKTIYISVVLGIMGLLFSQCNQKEFLSVANPSATDDAFVTSS
                     VSEAFKTLSWCYAEYRKTDAAGGNYNWNDPCSDAEYYPEYNSNNGRIGYLRPAEFTVD
                     GRKTQFNSLYQVLGRCARIADIIGSKTEYLEAKEAGVTDDWTQLYGEAWTFYCYCYFE
                     LVRHFGDVPFGIENSIVESYGLTSRFEILDKIIAKLKEVEPLMYDLGQGGITAERMSR
                     TFAHQLIAEAALYSGAYQTIRTDSIAQNLYGDIAFTAKYTDNAVKAAYVRRNDYKDYY
                     RLAQTHFRLVLGERKGSLSFITTDERDYCNNPFQRGFQYMADKQVSPESIWEAGNIAP
                     NQSERPYSQGRPSDGGGSNAAPCKVFSGIRIIPTFYYTGYEDGDKRMDPSMVVTGSDG
                     KGNEKIINFKSGSRLSGGIAVNKWDDNRMNPPYVVKQRNSGMNYVMRRISNTMLLLAE
                     VDALLGDETEALSIVNQIRDRAFGDDQHRLSVSGDALKEAISREVAREFVGEGDIRWE
                     MIRTGTMTDRAKKVRRDIKTMIAGLEADGYYTFANGRVISNYIWTKPVNKASKLTYDR
                     VDGDPALVPGWRGVYDYSTIPAVASIVTGTDNNLAIKGLFEYIDPTGAEAAALEADGY
                     TKTNWGIDIISGKNQLWDYNILSGIELTDVPLYYHSIPLETMQQSQGRVTNGYGLPQL
                     "
     gene            3003..4862
                     /locus_tag="PKY68_05385"
     CDS             3003..4862
                     /locus_tag="PKY68_05385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOH24504.1"
                     /translation="MENTLLNQIQEGVYQDESIVTLDWREHIRLRPGMYIGKLGDGSN
                     PDDGVYVLLKEVLDNSVDEYMMGYGKTIEVNISEGFVSVRDYGRGVPLNKVVDVSSKM
                     NTGAKYDSVAFKKSVGLNGVGIKAVNALSSFFRIQSIRDRQSKTVDFERGLIIKDKAL
                     EASEEDNGTLVEFTPDATLFKNYEYRDMHIERLIKNYVFLNSGLTIIYNGQKFFSKNG
                     LQDLLNENMTSESLYPIIHLSGYDIEIALTHGNQYGEEYYSFVNGQHTTQGGTHQTAF
                     REAVTRTIKEYYNKNHDYADIRMGMIAAVSIKVEEPVFESQTKTKLGSRDMGPKGPSI
                     SKYIGDFLKKELDNYLHKHPETSDILLKKITESEKERKAISNVTKLARERAKKANLHN
                     KKLRDCRFHLNDSKGENLEETSIFITEGDSASGSITKSRDVNYQAVFSLRGKPLNSYG
                     MTKKVVYENEEFNLLQAALNIEDGLEDLRYNKVIIATDADVDGMHIRLLLMTFFLQFF
                     PDLIKKGHVYILQTPLFRVRNKKEVRYCYTDEERLEAIEALGPNPEITRFKGLGEISP
                     EEFKHFIGKDMRLDKVNLRKEDLVKELLEFYMGKNTPERQNFIIDNLVIEEEV"
     gene            4859..5323
                     /gene="coaD"
                     /locus_tag="PKY68_05390"
     CDS             4859..5323
                     /gene="coaD"
                     /locus_tag="PKY68_05390"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOH24505.1"
                     /translation="MRTVLFPGTFDPFTLGHESIVKRSMQFFDRLIIAIGINDQKKPY
                     YSLDKRLEQIAACFPKQSNIEITHYTGLTGDFARQKNCQAILRGVRSVADYEYEKTLA
                     DFNYKAFGLETFFLYSKPELSMVNSAMVRELLRHGKDVSSYVPAALLPLLDL"
BASE COUNT         1571 a         1149 c         1263 g         1390 t
ORIGIN      
        1 gatgattttg attataatac cgtagaagga ttgtattacc tgaaagacgg agtccccgat
       61 atcagcccca atattaccgg tgttatgcat gggatagcta ctcccacagg gcaaaacgcc
      121 tatccgggta tggctaaata tagcgatacg gacggaagtg gtatggttga cggagaggat
      181 tttaccatca ttggagacat gaatgccaag cataccggtg gttttcatat cacagcccaa
      241 tacaagcaat tcgatttggg cctgtatttc aactggagtt acggcaatca agtgtataac
      301 gtcaacagaa tggcttcttt gatgggttat aaagaatcgg gtgtttatca aaacaagctg
      361 gtggtcttgg cttcgagtta taaaatctac gatatagtca acgggcaatt ggttagatac
      421 aacactcccg aagagttggc agccttgaat aaaaatgccg aatatccctt gtgttataat
      481 gaatcgggtg tggtgtcgtc gctgggtatt gaggacggtt cttatctcag attgaatacg
      541 ctcaatttcg gttatacttt gccaaagagc gtatcgacta agcttaaaat caataatatg
      601 cgcttgtttt tcacagctta caacctgttg acattgacca aataccaagg tcttgatccg
      661 gaagttaaca caaatgagcg tgttaatcac gccgtttacc cgacaccagg actcgactgg
      721 ggtacctatc cccgtccgcg ttccttagtt gttggtgcaa acatttcctt ttaactaata
      781 aacacaaaca actatgaaaa agaaaactat ttatatttcc gttgttttgg gaataatggg
      841 cctgctcttt tctcaatgca atcagaaaga atttttatcg gtagcaaatc cttcggccac
      901 ggacgatgcg tttgttactt cttcagtttc tgaagcattt aaaactttgt cctggtgtta
      961 tgccgaatat cgcaaaaccg atgctgcggg cggtaactat aattggaacg atccctgctc
     1021 ggatgcagag tattatcccg aatacaactc caataacggc cggatcggtt atttgcgtcc
     1081 ggctgaattt acggtagatg gccgtaaaac acagttcaac agcctttatc aagtcctggg
     1141 ccgttgcgcc cgtattgccg atatcatagg ttctaaaacc gagtatctgg aggctaaaga
     1201 ggccggcgtt acggacgatt ggactcagtt gtacggtgaa gcatggactt tttattgtta
     1261 ctgctatttt gaactggttc gccatttcgg agacgttccg tttggtatcg aaaattcaat
     1321 tgtagaaagt tatggtctga cctctcgttt tgagatattg gacaagatca ttgccaaact
     1381 caaagaggtt gagcctttga tgtacgacct gggtcaggga ggcatcacag ccgaacgtat
     1441 gtccaggact tttgctcatc aattaatagc ggaagcggct ctgtattcgg gagcatatca
     1501 aaccattcgt accgattcga ttgctcaaaa cctgtatggc gatatagctt ttaccgccaa
     1561 atataccgac aatgctgtca aagccgctta cgttcgtcgt aacgattaca aagattacta
     1621 tcgacttgcg caaacacatt tccgtcttgt tttgggagaa agaaagggta gtttgtcttt
     1681 catcacaaca gacgaaagag actattgcaa caatccgttc caaagaggtt tccaatatat
     1741 ggcggataaa caagtatcac ccgaatccat ttgggaagcc gggaacatag caccaaacca
     1801 aagcgaacgg ccttacagtc agggtcgtcc ttcggacgga ggaggaagca atgcagcccc
     1861 atgcaaggtc tttagcggaa tacggatcat accgaccttt tattacaccg gttatgaaga
     1921 cggcgataaa cgcatggacc cgagtatggt ggtaaccgga agcgacggaa aggggaatga
     1981 gaaaatcatc aacttcaagt ccggttcaag gttaagcggc ggtattgccg taaacaaatg
     2041 ggacgacaat cgcatgaatc ctccttatgt ggtgaagcaa cgcaattcag gaatgaatta
     2101 cgtgatgcgc cgtatttcca acaccatgct tttgttggcc gaggtcgatg ccctcttagg
     2161 cgatgaaacg gaagctctta gtatagttaa ccaaatcagg gatcgggctt ttggcgacga
     2221 tcaacaccgt ctttcggttt cgggtgacgc tttgaaagaa gccatctcca gggaagttgc
     2281 tcgcgagttt gtcggcgaag gcgacatccg ttgggaaatg attcgtaccg gaacgatgac
     2341 cgaccgggcc aagaaggtaa ggcgagacat aaaaactatg attgcaggct tggaggcgga
     2401 tggttactat actttcgcga atggacgagt aatttccaac tacatatgga caaagccggt
     2461 taataaagca agcaaattga cctacgaccg tgtagacggc gatccggcct tagtgccggg
     2521 ctggcgggga gtgtacgact attctaccat accggcagtg gcttcgattg tgacgggaac
     2581 agataataat ttagccataa aaggcctgtt tgaatacatc gatcctacgg gcgctgaagc
     2641 cgcagctttg gaagccgacg gctatactaa aacaaactgg ggcattgata tcatcagtgg
     2701 taagaatcaa ttatgggatt ataatatttt gtcgggtatt gaattgacag atgtacctct
     2761 ctattatcat tccatacctc ttgaaacaat gcaacaatcg caaggtcgag taaccaacgg
     2821 ttatggtttg ccacaactgt aatcttttga taaacactta atagcatccg ccacgttcgg
     2881 ctgtaaaaga cgaacgtggc ggatgtcttt tttgcaacaa gtgcaagagt gttttacaca
     2941 tagagccgaa tttttcttac ctttgcacat taatcaggta agttgaaata agtagaagta
     3001 atatggagaa tacgctattg aatcaaattc aggaaggagt ttatcaggac gaaagcatcg
     3061 ttacactcga ttggagagag catatacggc tgaggccggg tatgtatatc gggaaattgg
     3121 gagacggcag taatcccgac gacggtgtgt acgttttgtt gaaagaagtg ctcgacaata
     3181 gcgtggacga atatatgatg ggttacggca aaaccatcga agtcaatata agcgaaggtt
     3241 ttgtctccgt cagagattac ggcaggggag ttcccctgaa caaggtggtg gatgtttctt
     3301 ccaaaatgaa tacaggtgct aagtacgatt cggtagcttt caaaaaatcg gttggactca
     3361 atggtgtggg tatcaaagcc gtgaatgctt tgtcttcttt ttttcgcatt cagagcatac
     3421 gcgatcgcca aagtaaaacc gtcgattttg aacgtggcct gataatcaaa gacaaggccc
     3481 tggaagctag cgaagaagac aacggcactc tggttgaatt cacccccgat gctaccctgt
     3541 tcaaaaatta cgaatacagg gacatgcaca tagagcggct gattaagaat tatgtcttcc
     3601 tgaattccgg gcttaccatt atttacaacg gtcaaaaatt cttttccaaa aacggtttgc
     3661 aggatttgct caacgagaac atgacttccg agagcttgta tccgattata catttaagcg
     3721 gctacgacat cgaaatagcc ctgacccacg gcaaccagta cggcgaagaa tactattcct
     3781 ttgtcaacgg gcagcacacc actcagggtg gtacgcatca aacagccttc agggaggccg
     3841 tcacccgcac catcaaggaa tactacaaca aaaatcacga ctacgccgat attcgcatgg
     3901 gcatgattgc tgccgtcagc atcaaggtgg aagaaccggt gtttgaatcc cagaccaaaa
     3961 ccaaactggg ttcgcgcgat atgggcccca aaggtccttc gatcagcaaa tacatcgggg
     4021 attttctcaa gaaagaactg gacaattatc tacataaaca tcccgagacc tccgatatat
     4081 tactaaagaa gatcaccgaa tcggaaaaag agcgcaaagc catatctaac gtgaccaagc
     4141 tggcccgtga acgagccaaa aaagcgaatc tgcacaacaa aaagttgcgc gattgccgtt
     4201 ttcatttaaa cgacagcaag ggagaaaacc tcgaagagac cagcattttt attactgagg
     4261 gcgattcggc cagcggttcc attaccaaaa gccgtgacgt taattatcag gccgttttca
     4321 gtttgagagg gaaacctcta aacagctatg gcatgaccaa aaaagtagtg tacgaaaacg
     4381 aagaattcaa ccttttgcag gcagcgctga atatcgaaga cggactggag gatctgcgtt
     4441 acaataaagt gattattgcc accgatgccg atgtggacgg catgcatata cgcctcttgc
     4501 tgatgacttt ctttttgcag tttttccctg acctgatcaa aaaaggccat gtgtatatct
     4561 tgcagacccc cttgttcaga gttagaaaca aaaaagaggt acgttattgc tataccgacg
     4621 aggaacgtct cgaagccatc gaagccctgg ggcccaatcc cgagattacc cgctttaagg
     4681 gtctgggtga gatatcccct gaggagttta agcattttat aggcaaagac atgaggctgg
     4741 ataaggtgaa tttgcgcaaa gaagacttag tcaaggaatt actcgaattt tacatgggta
     4801 agaacactcc cgaacgtcag aatttcatta ttgataatct tgtcattgaa gaagaggtat
     4861 gagaacggtt ctttttccgg gcactttcga tccctttact ctgggacacg aatccatcgt
     4921 aaagcgaagt atgcagtttt tcgaccggct tattattgcc atcggcatca acgatcaaaa
     4981 gaagccgtat tattcactcg ataaacgcct ggaacaaata gctgcctgtt ttcctaagca
     5041 aagcaatata gaaatcactc attacaccgg tcttacggga gattttgccc ggcaaaaaaa
     5101 ctgtcaggct attttgcggg gagtgcgttc cgtggccgat tacgaatacg aaaaaacatt
     5161 ggccgatttc aactataagg cctttgggct ggaaactttt ttcttgtatt ccaaacctga
     5221 attatccatg gttaattcgg caatggtcag ggaattgctt cgacacggta aagacgtgag
     5281 ctcatatgta ccggcagccc tgcttcccct gcttgatctt taattagaaa tcgatgaaaa
     5341 aaacattctt gatccttagc tcactattgc tct
//
LOCUS       DAOSGL010000141         5346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13632_length_5346_cov_4.195615, whole genome shotgun sequence.
ACCESSION   DAOSGL010000141 DAOSGL010000000
VERSION     DAOSGL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13632_length_5346_cov_4.195615"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PKY68_05395"
     CDS             complement(<1..738)
                     /locus_tag="PKY68_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOH24506.1"
                     /translation="MICQRLLLFSFCALCLLSANESPAQKAQNPVIFADVPDMSMMRV
                     GDTYYMSSTTMHMAPGVPVMTSKDLINWKITSYCYDILDEVDALNLVNGKSTYGRGTW
                     ASSIRYHKGLFYVSTFAQTTNKTYIYTTDDPEKGEWKKTSFAPAYHDHSLFFDDDGRI
                     YFIWGVGRLRIIELEADLSGVKEETERVLIENASAPAGSPMGLQSEGSQLFKVNGRYY
                     LFNICWPRGGMRTVVIHRASSLEGPWKD"
     gene            1062..1718
                     /locus_tag="PKY68_05400"
     CDS             1062..1718
                     /locus_tag="PKY68_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24507.1"
                     /translation="MRIKTFSTIVLLFCTMSVNAQISRELVQNYIDLELANYPQATLI
                     DLYKNYFQDAFGPGHLMNDTNAAKDYLIKELAETDYRDTILFQPLGIHRDFYRINLSL
                     VKNGTIALDTLLNGMKESSVLARKPELSDWIIEWNQVLDIIQAYKPEIPNLQEDIQTI
                     HRNLEKGIVVSHHSRQFTETYRPHYRIIHKSVVERWMTSERKGDRPGIQELIRLEPEK
                     "
     gene            complement(1766..2206)
                     /locus_tag="PKY68_05405"
     CDS             complement(1766..2206)
                     /locus_tag="PKY68_05405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOH24508.1"
                     /translation="MLIRRARKSDLEAVLKIENSSFEAERFTRSQLLYLMTKARGLFL
                     VLELEAEIAAYLSVLTNKKRRSLRIYSIAVDPAHRGMKLGQLLIEKAMAYALELQLPA
                     ISLEVSTRNSSAIALYTRNGFNTVEILKDYYGKGENALKMIKKL"
     gene            complement(2209..3684)
                     /locus_tag="PKY68_05410"
     CDS             complement(2209..3684)
                     /locus_tag="PKY68_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HOH24509.1"
                     /translation="MNNNNVLILLDSLRDWAPYYQTNSLLTVLDYLQYKNGEKSRKLV
                     INLSNDYSYNSEGYYCSLLAQTRGHQVIPGIDIINKLESGTGIRMDQNLQRICYQWIQ
                     AHETAEPTWSIVIYFGTCKEKGLEKIARFIFEHYPCPILKVTFNTRSRNQIESVQAFP
                     LRELNDEQQTAFAEALDRFSQRVWQQPRSNKTSRFSLAILYDPEEKFPPSNKQALKKF
                     SEIAKKMRIHTEFITEEDANRLMEFDALFIRTTTSLNHYTFHLSQLAAQHGMAVIDDP
                     LSIIRCTNKVYLNELFEKEKIPVPPSMLILKSRHPSFEEITENIGEPFILKIPDGSFS
                     IGMVKIETEEALNEALDELFEKSAILLAQAFTPSEFDWRIGVLNGEPLYACKYYMAKG
                     HWQIYHHFQSGRSYSGTVDTIPIYQVPRTVIETAVKAASLIGKGLYGVDLKMVKDKAM
                     VMEINDNPSIDHGCEDIVLGNELYYRILDHFERSLNNNERF"
     gene            complement(3875..3952)
                     /locus_tag="PKY68_05415"
     tRNA            complement(3875..3952)
                     /locus_tag="PKY68_05415"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3915..3917),aa:Ile,seq:cat)
     gene            4258..4863
                     /locus_tag="PKY68_05420"
     CDS             4258..4863
                     /locus_tag="PKY68_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HOH24510.1"
                     /translation="MSEKEISRILMTVWGLSYSRMKSGAYILILAEASGDRRLPIIIG
                     SSEAQAISFSLEKKTPPRPLTHDLMASMCHAFGLELKEVFISDCVEDVFYAELYFEHR
                     GEQITLGSRTSDAVALALRVGAPIYTTESMLRKRGMVFEETEQRGRQQMRIKKIRDEE
                     TWEDASDEELQAALQKAVEHEEYEQASLIRDELKKRKEAKS"
     gene            4868..>5346
                     /locus_tag="PKY68_05425"
     CDS             4868..>5346
                     /locus_tag="PKY68_05425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOH24511.1"
                     /translation="MPVFYAPDIADRTCLPPEESAHCVRVLRLKEGEEIRLADGKGHF
                     YRARISKADAKQCEVEMLEVLPQASDSIGLHLAVAPTKNADRMEWLAEKLTELGMPVV
                     LVYLGFLNADEMQDRSAPFVSDAEWKQLVQAHSTPLFDVDPWEKVWACNGQSLVPLIR
                     "
BASE COUNT         1401 a         1182 c         1336 g         1427 t
ORIGIN      
        1 gtccttccag gggccctcga gactggaggc acgatgtatc accaccgtgc gcataccacc
       61 cctgggccag caaatattga acaaataata cctgccgttt actttgaaca gttgagaacc
      121 ttcggactgc aggcccattg ggctgcctgc cggcgcgctg gcgttttcta ttaaaacccg
      181 ttccgtttct tccttcacgc cgctcagatc ggcctcgagc tcgatgatgc gtaaacggcc
      241 cactccccag ataaaataaa tgcggccgtc atcgtcaaaa aacaggctgt gatcgtggta
      301 agccggagca aacgaagtct ttttccattc gcccttttca ggatcgtcgg tggtgtatat
      361 ataggttttg ttggtggttt gagcgaaagt ggaaacgtaa aagagcccct tgtggtagcg
      421 tatgctgctg gcccaggtgc ctctgccgta ggtgctcttg ccgttgacca ggttcagtgc
      481 gtccacttcg tcgagtatat cgtagcagta actggttatt ttccagttaa tcaagtcttt
      541 tgatgtcata acaggcaccc cgggagccat atgcatagtg gtgctgctca tgtagtaagt
      601 atcgcccaca cgcatcatag acatatcggg cacgtcggca aaaataacgg gattttgggc
      661 tttctgagcg gggctttcat tggcgcttaa caaacataat gcgcagaacg aaaaaagcaa
      721 aaggcgttgg cagatcataa ggttaaattt tatatggttg attgaaacaa aaaactttaa
      781 aattcgcaac gaatatacat tttttctgcc tttgtaaaaa aaagctccct gaatatcggg
      841 cgtagattca acttcccaag gaaactttta attatattaa gaccgatggc cggctgctac
      901 ggcagcatta tcagtacctc ccgctcgttg taggtgaacg ggcttgcgga tattaacccg
      961 ggtgttgcgg cagtaatacc ggtgcttccg gctagtttag attcccccga tatcattgaa
     1021 atgattacct ttacgaagtt ttattgccaa gacatcagaa gatgcggata aaaacatttt
     1081 ccaccatagt tctcctattt tgtacgatga gcgtaaatgc acaaatcagc cgggagcttg
     1141 ttcagaatta tatcgatttg gaacttgcca attacccgca ggccaccttg attgatttgt
     1201 ataaaaatta ttttcaggat gcctttggcc cggggcattt aatgaacgat acgaacgcgg
     1261 caaaagatta cctgattaaa gaactggcag aaacggatta tcgcgatacg attcttttcc
     1321 aacccctggg gattcatcgt gatttttaca gaataaacct aagtctggtc aaaaacggca
     1381 ccatagctct cgacacgttg ctgaatggca tgaaagaaag ttcggtattg gcacgaaaac
     1441 ccgaattgtc cgattggatc atcgaatgga atcaggtact cgacatcatt caagcgtaca
     1501 aacctgaaat tccgaatttg caggaagaca tccaaacaat ccatcgaaat ttagagaagg
     1561 gaatcgttgt ttctcaccac agccggcaat tcaccgaaac ctatcgcccg cattatcgca
     1621 ttattcacaa atcggtggta gagagatgga tgacatctga acgaaaggga gatagaccgg
     1681 gaatacaaga attaatccgc ttagaacctg aaaaataaat taaagctcat tgcttcggaa
     1741 caaattgcag cgaagtgttg aatggtcaga gctttttgat cattttcagc gcattttctc
     1801 ccttgccgta gtaatctttc agaatttcca cggtattaaa accgtttcgg gtgtacagag
     1861 ctatggctga gctgtttcgg gtactcactt cgaggctgat ggccggtaat tggagctcca
     1921 gggcgtaagc catcgccttt tcgatcaaca gttgacccag tttcattccc cggtgggcgg
     1981 gatcaacggc gatggaatat atgcgcaggc tgcgacgttt tttatttgtt aaaacagaga
     2041 gataagcggc tatttccgct tcaagttcca gtaccagaaa caagccccgg gctttggtca
     2101 taagataaag caactgagag cgtgtaaagc gctccgcttc gaaggaactg ttttctattt
     2161 tgagcacagc ttccaggtcg cttttccttg cccggcgaat cagcatagtc agaatcgttc
     2221 gttgttgttc agtgatcgtt caaagtgatc caaaatgcgg taataaagct cgttgcccag
     2281 cacgatatct tcacagccgt gatcaatact gggattgtcg ttgatttcca tcaccatggc
     2341 cttgtctttg accatcttaa gatcaacccc gtacaggcct ttaccaatca atgaggccgc
     2401 ttttacggcg gtttcgatca cggtgcgcgg cacctggtaa ataggtatgg tatcgaccgt
     2461 gccgctgtag ctgcgtcccg actggaagtg gtgataaatc tgccaatgcc ctttggccat
     2521 gtaatatttg caggcataga ggggttctcc gttgagcacg cctatacgcc agtcgaattc
     2581 ggagggagta aaagcctggg ccagcagaat ggccgatttt tcaaagagtt cgtccaaagc
     2641 ttcgttcaga gcctcctctg tttcgatttt gaccatgcct atcgaaaagg aaccgtcggg
     2701 tatttttaag ataaaaggct cgccgatgtt ttctgtaatt tcttcaaacg aagggtgtct
     2761 tgatttgaga ataagcatgg aaggaggaac cggaatcttt tctttttcga acaattcgtt
     2821 cagatagact ttgttggtgc aacgaataat ggacaagggg tcgtcgatta ccgccattcc
     2881 gtgttgagca gccaattgcg ataaatgaaa cgtatagtgg ttgagcgagg tggtggtgcg
     2941 gataaacagc gcatcaaact ccatcaggcg attggcgtct tcttcggtaa taaattcggt
     3001 atgaatacgc atttttttgg cgatctcgct gaattttttc agcgcctgct tgttactggg
     3061 cggaaacttt tcttcgggat cgtaaagtat ggccaggcta aaacggctgg ttttattgga
     3121 acggggttgt tgccagaccc gctgactgaa acgatcgagg gcctcggcaa aggcggtttg
     3181 ctgctcgtcg ttgagttccc tcagcgggaa agcctgtacg ctttcaattt gatttctgga
     3241 tcgcgtattg aaagtgactt tcagaatggg acagggataa tgttcaaaaa taaaacgggc
     3301 tattttttcc aggccttttt ctttgcaggt gccaaagtag ataactatgc tccaggtggg
     3361 ctcggccgtt tcgtgtgcct gtatccattg atagcaaatt cgctgcaggt tttgatccat
     3421 ccggataccg gtgccgcttt cgagtttatt gataatatcg atgccgggaa ttacctgatg
     3481 tcccctggtt tgggccagca gcgagcagta atagccttcg gaattgtaac tgtaatcgtt
     3541 gctcagatta ataaccagct tgcggctctt ttcgccgttt ttgtattgca ggtaatccag
     3601 aacagtaagc aggctgttgg tctggtaata aggggcccag tcgcggaggg aatcgagcag
     3661 aatcaacaca ttgttgttgt tcatacaaat atctgataag cagcacatta taaacaccga
     3721 aaaagggctg ccttttcatt tttttcagcg ccccaaaggt agagaaattc ctgaataatt
     3781 gttccgggga gtaaaaaaat acagttacaa gtagatagga gggcaattat aacaaaaaag
     3841 cagtcacaat ttcatgtaac tgcttggctt tgattggtgg gcccaactgg acttgaacca
     3901 gtgaccccct gattatgagt caggtgctac taaccgactg agctatgggc ccgctttgga
     3961 actgtctccg gaaagcggct gcaatattac tgctttttag tgaaatagcc aagggcaaaa
     4021 cacaattttt cttttggatt ttgctcagtc atctcttatc tttgtacacg gttcggtaaa
     4081 ctctgtcaaa tcggcgttgt cgcttagtat acaggaatat ctgaaaagct tcagagcgct
     4141 cgaatcccgg tttttaggca taaacggctg acgctaaagc aagatgaaaa gcagatggta
     4201 actcaaagag cttgttatct gacaggactg aaccctcaaa ataaaaagaa tagtattatg
     4261 tcggaaaaag agatttcgcg tattttgatg accgtctggg gcctatccta cagccgtatg
     4321 aaatcgggtg cttacatcct gattttggcc gaggcttcgg gagaccggcg tttacccatc
     4381 attataggtt ccagcgaggc tcaggccatt tcgttttcgt tggagaaaaa aacgcccccg
     4441 cgtccgctta cccacgattt gatggcttcc atgtgccatg ccttcgggct ggaactcaag
     4501 gaagtgttta tttcggattg cgtggaagat gtgttttacg cggagctcta ttttgagcac
     4561 aggggagagc aaatcaccct gggttccagg acttccgatg ctgtggctct ggctttgcgg
     4621 gtaggcgctc ctatttatac aaccgaaagt atgttgcgca agcggggcat ggttttcgaa
     4681 gaaacagaac agaggggccg gcagcaaatg cgtatcaaaa agatacgcga tgaggaaacc
     4741 tgggaagacg ccagcgacga agaattgcag gcagcactcc aaaaggctgt tgagcatgaa
     4801 gaatacgagc aggcttccct gataagggat gaattaaaaa aacgcaaaga agccaaatct
     4861 tgaccttatg ccggtatttt atgctcccga tattgcagac cggacttgtc ttcctcccga
     4921 ggagtcggcc cattgtgtca gggttttacg cctgaaggaa ggggaagaaa ttcgtttggc
     4981 cgacggtaag gggcattttt atcgcgcccg catcagtaag gcagatgcga agcaatgcga
     5041 agtggaaatg ttggaagtgt taccccaggc ctccgattcc ataggcttgc atttggctgt
     5101 agctcccacc aagaacgccg atcgtatgga atggttggcc gagaagctga ccgagctggg
     5161 catgccagtg gtgctggttt accttggatt tttgaacgct gacgaaatgc aagaccgcag
     5221 cgcgcccttc gtaagtgatg ccgaatggaa acaactggtg caagcgcaca gtaccccatt
     5281 atttgatgta gacccatggg agaaggtttg ggcctgcaat ggccaaagcc tagtgccact
     5341 gattcg
//
LOCUS       DAOSGL010000142         5327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13708_length_5327_cov_3.995448, whole genome shotgun sequence.
ACCESSION   DAOSGL010000142 DAOSGL010000000
VERSION     DAOSGL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13708_length_5327_cov_3.995448"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PKY68_05430"
     CDS             complement(<1..537)
                     /locus_tag="PKY68_05430"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOH24512.1"
                     /translation="MKKMMLGIKKIWMLSLLMFLSVSVSVAQNDLAETVRGVVVSGDP
                     THELIQATIYVEGSKPGIGTVTDEQGNFTFRVPVGRQKLRFSSVGFLSQEVDILVNTG
                     KEVVLNVVLEPSFIELQGAEIVARHDKSRPMNKLSVTGARTFSTEETFRFAGSLGDPA
                     RMVRSFAGVIPANDSRNDI"
     gene            complement(547..846)
                     /locus_tag="PKY68_05435"
     CDS             complement(547..846)
                     /locus_tag="PKY68_05435"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOH24513.1"
                     /translation="MESVFLEPILKGGDIDVVFGGMVIDLRKTALPDTTVYLNVDAIF
                     GGITLYIPEDWCVKSKFNPVFGGYSDKRASIVVDDKSDSRLVLQGSLIFSGCTVQ"
     gene            complement(874..1317)
                     /locus_tag="PKY68_05440"
     CDS             complement(874..1317)
                     /locus_tag="PKY68_05440"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOH24514.1"
                     /translation="MNENIFNKKGNNTRSLVVVLGAALVLTGLTLLAFNFGWLNPAFK
                     SVIFSWPMLFILFAVVGFSKRQILLPVLFLLIGVFFLLPRLEIVYPGILGEAGRGGFA
                     KNFWPFLLIIIGLMFIVGVATKRKKKVSFTQDVVDTQAVTSGESG"
     gene            complement(1365..>1713)
                     /locus_tag="PKY68_05445"
     CDS             complement(1365..>1713)
                     /locus_tag="PKY68_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24515.1"
                     /translation="NRTVGRNYGKSFTPSAWTFPAPHAYLVARRGTARVYYPREIGKL
                     ESFEQLLKVKEEIKSILEDYSFEHYTVETELDEESSYVSFDKSLYYGHTSISDKNLIT
                     FYYCCNSFLHGLS"
     assembly_gap    1714..1723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1734..2333)
                     /locus_tag="PKY68_05450"
     CDS             complement(1734..2333)
                     /locus_tag="PKY68_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24516.1"
                     /translation="MKTRISFLGKYASLLLLLSLILSTGCSLGSRIYKKYDQVQKVDR
                     YKLDLNYPDFNLRSPLLYMEQSIVKEVSAKESKYSVYDVLVLNSASYRVKEELYIIVE
                     DEIFKPAVTGLEYDNAREIREKSEEVVRADSSTVKVVTGYTENNKKITRFTYTLDNDV
                     IERIRKASIVLFRYYAGPDMVTIKLKGLKLKRVKRLIEK"
     gene            complement(2421..5120)
                     /locus_tag="PKY68_05455"
     CDS             complement(2421..5120)
                     /locus_tag="PKY68_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HOH24517.1"
                     /translation="MKRLFLTGCVLSLGFCCVFAQPLAFPGAEGFGANSRGGRGGKVI
                     YVSNLNDEGPGSLREAVEQEGPRTVVFSVSGIIDLKTRLNIEHPYITIAGQTAPGDGI
                     CLRGETLRIAADHVIVRYLRVRLGDGKHGQGSLQGKDAISISSGRHIILDHCSAGWSL
                     DEVLSASSRTPDLSKVTVQWCFITEALNPDGHSFGSLLRGTGGAKYSFLHNLYAHNRG
                     RNPRPGNYDSNPHDRDTLGLLLDFRNNVVYNWGGEHAAYNADKVSVTRLNFVHNYFVP
                     GQDSKASGIVYSTGSPYNRAYFAGNYYMDELPDDPWKLVKFNESWTQKEISAYKQDQP
                     FESGIVQTDEAPAAYEKVLETGGAVLPKRDAADQRIVKDVRQRTGRIIQSQEEVGGWP
                     ELKSAEAPLDTDLDGMPDEWEKKQGLNPKDPEDRNKLNAEGYTMLEQYLNSIGSFQPV
                     ASGKHRVIVLTDIENEPDDAMSLVRFLCYCNQWDVEGLVATTSVHQQKETAAWRIKEI
                     LQAYGRVRDNLEKHEPGFPTADYLLSVLREGRPDYGMRAVGKGMDSPGSELIIQAVDR
                     PDSRPVWVLVWGGPNCLAQALWKVKETRSAKELEHFVSKLRVYTISDQDDSGPWIRKT
                     FPELFYIASPGFHRYGGYHYATWSGISGDYFHARCAGGDFSLVDNDWVDKNVQNKGPL
                     GEQYPDIKFLMEGDSPSFMFLIDNGLGDPEHPDWGSWGGRYEYYLPKFEKWFLEPETR
                     PLWTNAMDEVLGSDGRWHTGNHATIWRWREHFQNDFSARMDWTIKTYEQANHPPVPKL
                     GHEAYLRAKAGERVNLSAAGSSDPDGDSLRYNWFMYGEPGTFSMGTARTGNPLIINDS
                     DKQEAWFIAPEKARMGTMHIILAVSDDGHPSLTRYKRVIVEIQ"
BASE COUNT         1419 a         1374 c         1096 g         1428 t
ORIGIN      
        1 aatatcattt cgcgaatcgt ttgcgggaat cactcctgca aagctacgca ccatacgtgc
       61 cggatcgcca agcgaaccgg caaatcgaaa ggtctcctcc gtgctaaaag ttcttgctcc
      121 ggtaacagac aacttgttca tcggccggct tttatcatgt cttgccacta tctctgcgcc
      181 ttgtagttca ataaaggaag gttccagcac cacattcagc acaacttcct tacctgtatt
      241 taccagtata tcaacttctt gtgataaaaa ccccacactg ctgaatctta acttttgtct
      301 tccgacagga accctgaaag taaagttgcc ttgttcatcc gtcactgttc ctattccggg
      361 tttgctcccc tcgacatata ttgtcgcttg aatcaactca tgagtaggat cgcccgaaac
      421 cactacgcct cttactgttt ccgctaaatc gttttgtgca acactaaccg ataccgacaa
      481 aaacataaga agcgataaca tccatatttt ttttatgcca agcatcatct ttttcattga
      541 ataaaactac tgaacagtgc aaccggaaaa aattaaagaa ccttgcaaaa cgagccgact
      601 gtcgcttttg tcatctacaa ctatgctcgc tcgcttatcg ctgtagcctc caaagacggg
      661 attgaacttt gattttacac accaatcttc aggaatataa agtgttatac ctccaaaaat
      721 tgcatccaca ttgagataca cggttgtatc gggcaaggcg gttttccgta aatcgatcac
      781 cattcctcca aaaaccacat caatatctcc gcctttaaga ataggttcca gaaaaacgct
      841 ttccaaacct ccaaaaacaa catctcttat tattcagcca ctttctccag atgtaacagc
      901 ctgcgtatca acgacatctt gcgtgaaaga aacttttttc tttcttttgg tcgcaacccc
      961 gacaataaac atcaaaccga taattatcag caaaaacggc caaaaatttt tggcaaaacc
     1021 tcctctacct gcttcgccta atatcccggg ataaacaatc tccagacgtg gcaaaaggaa
     1081 aaaaactccg atcaataaaa aaaggacagg aagtagtatt tgtcttttag aaaacccgac
     1141 aactgcaaac aggataaaaa gcatgggcca agaaaagata accgatttga atgcgggatt
     1201 taaccaacca aaattaaatg caagtaatgt cagacccgtt aataccaaag ccgcaccaag
     1261 tacaaccacc aagctacgtg tattattccc ttttttgtta aatatgtttt cattcatagt
     1321 tcaatttgtt ttagttcgac attctaatta caaactgtgc aaagttagct aagcccatga
     1381 agaaaactgt tacaacaata ataaaatgtt attaaatttt tatcggaaat tgatgtatga
     1441 ccgtaataga gtgatttatc aaaagagacg tagctgcttt cttcatccaa ttccgtctcg
     1501 acagtatagt gttcaaaact gtagtcctcc agaatggact taatttcttc ttttactttc
     1561 agaagttgtt cgaaactctc taattttcca atttcacgtg ggtagtaaac acgtgccgtt
     1621 ccccgtcgag cgaccagata tgcgtgtggt gcagggaatg tccatgccga tggtgtgaat
     1681 gatttgccat aattacgtcc tactgtccgg tttnnnnnnn nnngcattac tcgctatttt
     1741 tcgatcaatc ttttaactct cttcaatttg aggcctttca gcttgattgt taccatgtcc
     1801 gggccggcat aatagcgaaa caagacgatg ctggctttac ggatgcgctc aattacgtcg
     1861 ttatcgagtg tataagtgaa acgggtaatt tttttattgt tctcggtata gccggttacc
     1921 acttttaccg tcgaagaatc ggctctcacc acctcttcgc ttttctcccg tatctccctg
     1981 gcattatcgt attcgaggcc ggtaacagca ggcttaaaaa tctcgtcttc tacgatgatg
     2041 taaagctcct ctttcactct gtaactggca ctgttcaaaa ccaagacatc gtaaacgctg
     2101 tatttactct cctttgcaga gacctctttt acaatggatt gctccatata caacaagggt
     2161 gacctcaggt taaaatccgg gtaattcaag tccagtttgt aacgatctac tttttgaacc
     2221 tggtcgtatt ttttgtagat tctgctgccc aaagagcaac cggtcgacaa aataagcgaa
     2281 agcagcaaga gcaggcttgc atacttgcct aaaaaggata tccttgtttt catctggtga
     2341 atttgaataa aataacagga aacaaacagc cagatcgaaa gggtcttttc cggtaactac
     2401 ctgcaaacag ggtatttaag ctattgtatc tcaacaatta ccctcttata cctggtcagc
     2461 gagggatgac cgtcgtcgct gacagccagt atgatgtgca tggtgcccat acgggctttt
     2521 tcgggagcaa taaaccaggc ttcctgcttg tcggaatcgt ttattatcaa aggattgcct
     2581 gttctggctg tgcccatgga aaaggtgccg ggttctccgt acataaacca gttgtacctg
     2641 aggctgtcac catcgggatc ggaactgccc gctgcgctta aatttacccg ctcgcccgct
     2701 tttgcccgga ggtaggcttc gtgcccgagt ttgggaaccg gaggatgatt tgcctgttca
     2761 taggttttga ttgtccaatc catacgagcc gaaaaatcgt tctgaaaatg ttctctccaa
     2821 cgccagatgg tggcatgatt gccggtgtgc caacggccgt cgctgcccag cacctcatcc
     2881 atggcgttgg tccacagcgg ccgggtttcg ggctccagaa accatttctc aaatttgggc
     2941 aaataatact cgtagcggcc accccagctc ccccaatcgg gatgctccgg atcgcccagc
     3001 ccattatcta tcaagaacat aaagcttgga gaatcgcctt ccattaaaaa tttaatatcg
     3061 gggtattgct ctcccagggg gcccttgttt tgaacattct tgtccaccca atcgttatcg
     3121 accagactga aatcgccgcc ggcgcaacga gcatgaaaat aatctccgct gattccgctc
     3181 caggtggcgt aatgataccc cccgtagcgg tgaaaacccg gactggcaat ataaaacagt
     3241 tccgggaaag tcttgcgtat ccaggggccg ctgtcgtcct ggtcggaaat agtatatact
     3301 ctgagttttg aaacaaagtg ttccaattct tttgctgagc gggtttcttt caccttccac
     3361 aaggcctgcg ccagacagtt ggggcctccc cagaccaaaa cccagaccgg ccttgaatcg
     3421 ggacgatcca ccgcttgaat aatcaattcc gaacccggcg aatccatgcc cttaccaacg
     3481 gccctcatgc cgtagtcggg ccttccttcc ctgagcacag acaataaata atctgccgtg
     3541 ggaaaacccg gctcgtgttt ttccagattg tccctgaccc ggccgtaagc ctgcagtatt
     3601 tctttgatgc gccaggcggc cgtttctttt tgttgatgga ccgaagtggt agccaccaaa
     3661 ccctctacat cccactgatt gcaatagcat aaaaagcgca ccagcgacat agcgtcgtcg
     3721 ggttcgtttt cgatgtcggt caatacgatt actctgtgtt tcccggaagc aaccggctga
     3781 aaacttccaa tactattcaa atattgttcc agcatggtgt agccttcggc attcagctta
     3841 ttccggtctt cggggtcctt gggattcaga ccctgtttct tctcccattc gtcgggcatg
     3901 ccgtctaaat cggtatcgag aggagcttcg gcgcttttta attcaggcca tccgcctacc
     3961 tcttcctggc tttgaataat ccggcccgta cgctgacgca cgtcctttac tatgcgctga
     4021 tctgccgcat cccgtttagg aagtacggct ccgcctgttt cgagcacttt ttcgtaagct
     4081 gcgggtgcct catcggtttg aacaattccg gattcaaaag gctgatcttg tttgtaggca
     4141 gagatctctt tctgagtcca gctttcgttg aactttacca acttccatgg atcatcgggc
     4201 aactcatcca tgtagtaatt ccctgcaaaa taggcacgat tatagggtga gccggtagaa
     4261 taaacaatgc cgcttgcttt ggagtcctgc cccggaacga agtaattatg tacaaaattc
     4321 aagcgggtta cactcacttt gtcggcattg taagcggcat gttctccacc ccagttgtag
     4381 acgacgttgt ttctgaaatc gagcaacagc cccagagtat ccctgtcgtg cggattggaa
     4441 tcgtaattgc ccggccgggg attgcgccct ctgttgtgag cgtagagatt atgcaggaaa
     4501 ctgtatttgg ctcctcccgt tccccggaga agagaaccaa aactatgtcc gtcgggattc
     4561 agagcttcgg tgataaaaca ccattgaacg gtaaccttgc tcaaatctgg cgttctgctc
     4621 gaagcagaca aaacctcgtc caggctccag ccggcagaac aatgatccaa gataatgtgc
     4681 cgaccggaac tgatactgat agcatctttg ccctgcaaac tgccctgtcc gtgcttaccg
     4741 tcgcccagcc tgacccggag gtaacgaacg atgacatgat cggctgctat gcgaagggtt
     4801 tcgccgcgta aacaaattcc gtctcccgga gctgtttgcc ccgctatggt aatataggga
     4861 tgctctatgt tgagcctcgt tttcaaatcg ataatgcccg aaacggaaaa cactaccgtg
     4921 cgcggacctt cctgctcaac ggcttccctc aggctgcccg gtccctcgtc gttaagattg
     4981 gacacataaa tcacttttcc tccgcgacct ccgcgggaat tggcaccaaa gccctctgct
     5041 ccgggaaaag ccaaaggctg ggcaaaaaca caacagaaac caagcgataa aacgcatcct
     5101 gtcaaaaata atcttttcat aatggttata aaatgaatac aaacccatct gtttttcaaa
     5161 aaacaagaca ataatcaaag ctcctgcatc accaaataaa gcatgtacga tgcgtaagag
     5221 acaaacaaaa cagcagcctc ccaacggctc agctgttttc ttttcccgat aagtacggcc
     5281 atcagcagga aaacggtacc tccaatcagc accaacaagt cctgatt
//
LOCUS       DAOSGL010000143         5310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13767_length_5310_cov_3.770695, whole genome shotgun sequence.
ACCESSION   DAOSGL010000143 DAOSGL010000000
VERSION     DAOSGL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13767_length_5310_cov_3.770695"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKY68_05460"
     CDS             complement(<1..614)
                     /locus_tag="PKY68_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH24518.1"
                     /translation="MSQQSNSNLKIGMTFMASIFFIFGFITNFNIAMKDQVQLAFTLS
                     NFGAQLVNFAFFIAYAVFSFLCGFIIKRIGYKNGVIAGLVLVAAGSYTFFPAVAALSY
                     PLFLLAVFIMASGVVFLQTAANPYVAALGPQETAPARLNLTQALNGVATTIAPFLAGV
                     LVLAPAVFAINSGSTPADAAKFVQGPFLVIGTLVLLIAIGISFI"
     gene            950..1837
                     /locus_tag="PKY68_05465"
     CDS             950..1837
                     /locus_tag="PKY68_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24519.1"
                     /translation="MKLKDLFVPAWKHSNPNVRLKAIEKNNYDENILSCIIKNTDEEI
                     DVAFAAIDKLQNPELCEKLFQTVYGKNADEKRKKILQKITDEKRIHNIYWKIRNSGGK
                     SFLEIDLEERLFEIWKSEVEQTHDTDTLIAIAKKSLNHIQGSYKSCCSYGITHDAIKK
                     IPDLKKLSAWVLSQFALNQELHYSILNGLSERLKQKDSGKFFSTIDNTELLIFLYKNG
                     GTALVSGVEKRLSQMGKKIRVDEYDKEGKCSSCEGVGGGDARIPYTDNDWKWERCEYC
                     NGTGREIFHIKEYFVESLE"
     gene            complement(1866..3401)
                     /locus_tag="PKY68_05470"
     CDS             complement(1866..3401)
                     /locus_tag="PKY68_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOH24520.1"
                     /translation="MRVLVIVFAAAMLMACSAGKQPTQINVNSVVIEDGKLSGKLSDD
                     GIVKIFMGIPYAAPPLGELRWKSPQPIKKWEGTRECVASPASAMQAKPAAYMCWSEEF
                     QPPVEPISEDCLYLNVWSPAKTTADKLPVIVWIHGGAFINGSGTVPLYNGEGMAKKGV
                     IFITINYRLGIAGFFAHPELSSESEMKVSGNYGILDQIAALKWVKKNIEAFGGNPGNI
                     TIAGQSAGSFSVNVLVASPKAKGLFHKAIAQSGGNFSLTNNRSEALEAQEAKGLRVTE
                     SLGLKSIEEMRSLPAESLLSINERFGLTTDNVVIPSIYETFEKSEHNDTPLLTGWNAD
                     DGVSFGPGPSAEKFTEMARNNYGEKAERFLQLFPAATDKQAEASQKLLSQLYFGWQNY
                     TWARMQTRKGKNPAYVYFFTHVPPGEQPYYGAFHSAEFAYALHTLKFWNRPFSEPDFQ
                     LEEIMSSYWANFAKTGNPNGEGLPEWPVFNPDKPEVMDLGDNVKAISLPHQEQLSFLD
                     EAQ"
     gene            complement(3412..4584)
                     /locus_tag="PKY68_05475"
     CDS             complement(3412..4584)
                     /locus_tag="PKY68_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOH24521.1"
                     /translation="MNKLTVILMLLFTSIGYYADAQQSLWNDSKLVSPQINNDNSVTF
                     RVEAPGAREVKIIGDWIPSAGQSTGGVTMKLGENGVWSYTSAPLASDLYSYLFIIDGI
                     KTNDPNNVYQIRDVASIFNVFIVKGGKGDLYSVNDVPHGTVSRCWYNSPGLSKDRRIT
                     VYTPAGYEKSKEKYPVLYLLHGMGGDEEAWIALGRTAQILDNLIAQGKARPMIVVMSN
                     GNASQQAAPGEAAAGMHKPTMMLPNTMDGTFETSFPDIISFVEQNYRVIAEKKGRAIA
                     GLSMGGFHSLHISRFYPNTFDHIGLFSAAIMPDQQAKSKVYDKFDETLSLQQKNGYKT
                     YWIGIGKTDFLYQANTDFRKKLDSIGMKYTYLETEGGHTWTNWRTYLSEFSQIIFK"
     gene            complement(4706..5074)
                     /locus_tag="PKY68_05480"
     CDS             complement(4706..5074)
                     /locus_tag="PKY68_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOH24522.1"
                     /translation="MKALYTIGLLILSNTFMTLAWYGHLKFKEYKWFESLPLIMVVLI
                     SWGIALFEYFFQVPANRIGYKGNGGPFTLFQLKVIQEAITLIVFTLFAVFAFKTETFK
                     FNHFIAFVFIILAVYFVFKE"
     gene            complement(5097..>5310)
                     /locus_tag="PKY68_05485"
     CDS             complement(5097..>5310)
                     /locus_tag="PKY68_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOH24523.1"
                     /translation="ASCGCTTPSWTKDPVAPGQSGEIKVTYDTHRIGAFSKSVTVTSN
                     AVETPSVVLRISGEVKQNTEPQTSVN"
BASE COUNT         1641 a         1142 c         1072 g         1455 t
ORIGIN      
        1 ttaatgaaac taatccctat ggcaattagt aaaacaaggg tacctataac aaggaatggg
       61 ccttgaacaa attttgcagc atcggcaggg gttgaccctg agttaattgc aaacacagca
      121 ggagccaaaa ccagcactcc ggcaaggaaa ggggcaatag ttgttgccac cccgtttaac
      181 gcttgggtaa gattaagacg ggcaggagct gtttcttgtg gccctaatgc tgcaacatac
      241 ggattggcag cggtctgcaa aaacaccacc ccagaagcca taatgaaaac agccaaaagg
      301 aacaatggat acgacaacgc agctacagcc gggaaaaacg tgtaacttcc ggcagcaaca
      361 agcacaaggc cggcaattac tccattttta tacccaatac gtttgattat gaagccgcac
      421 aaaaagctga aaacggcata tgcaataaaa aaagcaaagt taaccagctg agccccaaaa
      481 ttggaaaggg taaacgccag ctgaacctgg tctttcatcg caatgttgaa attggtgata
      541 aaaccaaaaa tgaaaaagat ggacgccata aaggtcattc caatctttag gttactgttg
      601 gattgctgtg acatttgagt acttttaatt aatttgtttt atggtttgtg tgaatttacg
      661 tcgaaacgaa caaatttaaa aattaaatcg aaattgatct atgctctccg tcgtaaaact
      721 gcgttaacaa ttcctatttc cccaatatat ttttgacact caatcgggat cgttttccat
      781 tacagaaatc tttttcatat acgctacacc cgaacaatgg aacaacaaaa attaaaccct
      841 ttaaagagaa taattctcaa catgatccca aaataattca tacttttgaa taaagagaga
      901 cattaaacct ccagaaaatc ttttgggttt ttcagcaaat acaccgccaa tgaaacttaa
      961 agaccttttt gttcccgcat ggaagcacag taaccctaac gtacggttaa aagccatcga
     1021 gaaaaacaac tacgatgaaa atattctttc ctgcattata aaaaacactg atgaagagat
     1081 tgatgtcgct tttgcggcta tcgacaagct acaaaacccg gagctatgcg aaaaactttt
     1141 ccagactgtt tatggaaaaa atgcagatga aaaacgaaag aagattcttc aaaagattac
     1201 tgacgaaaaa cgcatccaca acatttactg gaaaatacgt aacagtggtg gaaaatcttt
     1261 tctggagatc gatctcgagg aaaggttatt tgaaatatgg aaatcggagg ttgaacaaac
     1321 ccacgataca gatactctca ttgccattgc caaaaaatcg ctaaaccaca ttcaaggttc
     1381 atataaaagt tgctgttcat acggtatcac ccatgatgcc attaaaaaaa tacctgattt
     1441 aaaaaaactg tcggcatggg tattgtctca atttgctttg aaccaagagc tacattattc
     1501 cattctgaat ggtttgtccg aaaggctgaa acaaaaagat tctggaaaat tcttttcaac
     1561 gatcgacaat accgaattac taatctttct atacaaaaac ggagggactg ctcttgtttc
     1621 aggtgttgag aaaaggctat ctcaaatggg aaaaaagatc agggttgatg aatacgacaa
     1681 agagggaaaa tgctcttcgt gcgaaggagt aggcggagga gacgccagga ttccctacac
     1741 cgataatgac tggaaatggg aaagatgtga atactgcaac ggaacaggcc gcgaaatatt
     1801 tcacattaaa gagtacttcg tcgaaagcct cgaatagaat tcattactac aatcatatca
     1861 agttattact gagcctcgtc taaaaatgat agttgctcct gatgaggtaa agaaatggct
     1921 tttacattat cgcctaaatc cataacctcg ggtttatcgg gattgaagac gggccactcg
     1981 ggcaaccctt ctccattggg attgcctgtt ttggcaaagt tggcccaata actgctcatt
     2041 atttcttcaa gttgaaaatc gggttcagaa aacggacggt tccagaactt aagagtatgc
     2101 aaagcataag cgaattcagc cgagtgaaaa gccccatagt agggttgttc tcccggcgga
     2161 acatgggtga aaaaatatac gtacgccggg ttctttcctt ttcttgtctg catgcgggcc
     2221 catgtataat tttgccatcc aaaatatagc tgggatagca gtttttgcga agcctctgcc
     2281 tgcttgtcag ttgcggcagg aaaaagctga agaaaacgtt cggccttttc accataattg
     2341 ttcctcgcca tttctgtaaa cttttcggct gaaggacctg gtccgaatga aaccccatcg
     2401 tcggcattcc agcccgtcag caagggcgta tcgttatgct cacttttttc aaaagtctca
     2461 taaatcgatg gaatgactac gttatcggtt gtgaggccaa atcgctcatt tattgaaagt
     2521 aacgactcag caggcaacga acgcatttct tcaattgact tcaatcctaa tgattctgtt
     2581 acgcgcaatc cttttgcttc ttgtgcttca agagcttcgc tgcgattatt ggtaaggcta
     2641 aaattacccc cgctctgtgc aatagctttg tggaataagc cctttgcctt gggcgaagcc
     2701 accagcacat tcacgctaaa cgatccggcc gactgcccgg caatggttat gttccccgga
     2761 ttcccaccaa aagcttcaat gtttttcttc acccacttga gtgcagcaat ctgatcgagg
     2821 atcccataat ttccagaaac tttcatttca gactcagacg agagttcagg gtgagcaaag
     2881 aagcctgcaa tgcctagccg gtaattgatg gttataaaaa taaccccttt ttttgccatg
     2941 ccttccccat tgtataaagg aactgttcct gagccattaa tgaatgctcc cccatgaatc
     3001 cagactataa ctggcaattt gtcggctgtt gtttttgccg gactccaaac attaaggtaa
     3061 agacaatctt cactaatggg ttcaaccggt ggttgaaact cttcggacca acacatatat
     3121 gctgctggtt tagcttgcat agccgatgca ggggaagcaa cacattccct tgtgccttcc
     3181 cactttttaa tgggttgtgg cgatttccaa cgcaactctc ctaaaggggg tgcggcatag
     3241 ggtatgccca taaatatttt cacaatgcca tcatccgaaa gttttcccga aagttttcca
     3301 tcttcaatga ctactgaatt aacatttatc tgcgttggct gttttcctgc agaacatgcc
     3361 atcagcattg cagcggcaaa tacaattacc agcaccctca taacaaaata attacttgaa
     3421 tattatttga gaaaattctg acaaataggt tctccagttg gtccaggtat ggccaccttc
     3481 tgtttccaga taagtgtatt tcatgcctat cgaatcgagt ttttttctga aatcggtatt
     3541 tgcctgatac aaaaagtcgg ttttgccaat gccaatccaa taggttttat acccgttttt
     3601 ttgttgcagc gagagagtct cgtcaaattt gtcgtacact tttgattttg cctgttgatc
     3661 gggcattatg gctgctgaga acaagcctat atggtcaaag gtattgggat agaaacgcga
     3721 aatatgcagg gagtgaaaac cgcccatcga taagcctgca atagcccgtc cctttttctc
     3781 ggcaataacc ctataatttt gctcaacaaa agaaattatg tcgggaaaag aagtttcaaa
     3841 agttccgtcc atggtattgg ggagcatcat tgtaggtttg tgcattcctg ccgcagcctc
     3901 accgggagca gcttgttgcg atgcatttcc attgctcata accacaatca ttggcctggc
     3961 ttttccttgc gcgatcaggt tatcgagaat ttgagctgta cgcccaagcg cgatccaggc
     4021 ttcttcatcg ccacccattc catgcagcaa gtacagcacc ggatatttct ccttactttt
     4081 ttcatatcca gctggtgtat aaacggttat ccgccgatct tttgaaaggc ctggcgagtt
     4141 gtaccaacac cgtgaaacgg taccgtgagg tacatcgttc accgaataga ggtcgccttt
     4201 cccccctttt acaataaaca cattgaaaat tgaagctaca tcgcgaattt gataaacatt
     4261 gtttggatcg ttggttttta tgccatctat aataaaaaga tagctgtaca aatcgctggc
     4321 taacggagct gaagtatagc tccagacacc attctcaccc aatttcatcg taacaccccc
     4381 ggtggattgc cctgcagaag gaatccaatc gccaataatt tttacttcgc gggctcccgg
     4441 ggcttcaacc ctaaaagtta ccgagttatc gttgttgatt tgtggcgata caagtttcga
     4501 atcgttccac aaagattgct gtgcatcagc ataatagcca atggatgtga acagcaacat
     4561 taaaattaca gtcagtttat tcatggtaat ctaatctatc gttaattggt tgattaagat
     4621 aggtagaaat tttggcacga gagggaaaat aagataaaaa aagccgggtg aaaaatctcc
     4681 ccggcttttt ttattaggta tatttctatt ctttgaagac aaaataaacg gccagaatga
     4741 taaaaacaaa ggcaataaaa tgattgaact taaatgtttc tgttttaaag gcaaaaactg
     4801 caaataatgt aaaaacaatt aaggtaattg cttcctgaat aactttcaac tgaaaaaggg
     4861 taaacgggcc gccattgcct ttataaccaa tccggttggc tggtacctga aaaaaatact
     4921 cgaacagcgc aatcccccag ctaataagaa cgaccataat cagaggaaga gactcaaacc
     4981 atttgtattc tttaaactta agatgaccat accaggcaag cgtcataaat gtattggaaa
     5041 gaattagcaa tccaatagta tataatgcct tcatttacag atcaatcaaa agcaaattaa
     5101 ttaacactgg tttgtggttc tgtgttttgt ttcacttctc ccgaaatgcg caaaaccaca
     5161 gaaggggttt caacagcgtt tgacgttacg gttaccgatt tcgagaaagc gcctatgcga
     5221 tgcgtatcgt aagttacttt aatttctccg ctttggcccg gtgcaacagg atctttggtc
     5281 caactcggag tagtacagcc gcatgaagcc
//
LOCUS       DAOSGL010000144         5307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13782_length_5307_cov_3.629855, whole genome shotgun sequence.
ACCESSION   DAOSGL010000144 DAOSGL010000000
VERSION     DAOSGL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13782_length_5307_cov_3.629855"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..565
                     /gene="efp"
                     /locus_tag="PKY68_05490"
     CDS             2..565
                     /gene="efp"
                     /locus_tag="PKY68_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOH24524.1"
                     /translation="MATTADFRNGMCIEIDGAYYSIVEFLHVKPGKGAAFVRTKLKNV
                     QTGRILEKTFNSGVKVDEVRIERRPYQFLYQDDMGYNFMHSDTFEQINIAKESINGVD
                     FLKEGDICEVVVHAESETVLYADTPVHVELEVTYTEPGLKGDTATNALKPATVETGAE
                     VRVPLFIEVGDRIKVDTRDGSYVERAK"
     gene            727..1425
                     /gene="radC"
                     /locus_tag="PKY68_05495"
     CDS             727..1425
                     /gene="radC"
                     /locus_tag="PKY68_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOH24525.1"
                     /translation="MEQKNKFSIRDWSEEDRPREKMIKKGVGSLSDSELLAIILGSGS
                     TKESAVELAKRMLNSVDNNLSLLSKMSVQELRSAFHGVGQAKAVSILATFELGKRRQL
                     ADIPDKVQLNSSKKIYELMYPLMADLKHEECWAIYMNSSNRLIDMKKISSGGITETTV
                     DPRIILKHAIQHMATALVLCHNHPSGNIQPSDADNRLTKRLNEASKMMSICMLDHLVI
                     CDGKYFSYADQGLL"
     gene            1461..1778
                     /locus_tag="PKY68_05500"
     CDS             1461..1778
                     /locus_tag="PKY68_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOH24526.1"
                     /translation="MDNTFLEKEEAIVKMLRTVYDPEIPVNIYELGLIYKVDIDDASK
                     VTIEMTLTSPSCPMADFIIEDVRMKVQSLDFVGDVDINLVFEPEWNKDMMSEEAKLEL
                     GYL"
     gene            1783..2541
                     /locus_tag="PKY68_05505"
     CDS             1783..2541
                     /locus_tag="PKY68_05505"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOH24527.1"
                     /translation="MSTGGKIYFVTDAHLGLRADVPVAGNEKKLVRWLDSIQADCGAL
                     YMLGDMIDFWYEYKEVVPKGFVRFFGKLAEFTDAGIPVYWFAGNHDLWLFSYVQQEMG
                     IVVYKEAQELDILGKRFYIAHGDGLGYAPFSFRIMNGIFHSPFCQALFKILHPDLGIS
                     FGLRWSRHSYYKRKTMELAYLGEDKEHLIQFAKKHLATAKPAPDFYVFGHRHILLDLQ
                     LTRDCRLVIPGDWIREFSYATFDGEDLVLDHFEE"
     gene            complement(2564..3745)
                     /locus_tag="PKY68_05510"
     CDS             complement(2564..3745)
                     /locus_tag="PKY68_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOH24528.1"
                     /translation="MENQNKPYTFDRVVRIVIALLVITGLGLLIYRLSAVLLPFLVAW
                     FLAYFIRPAVLFFQHKLKLKNRVLSIVVTLLLIFGLLGMIIHFLKTPVLNEIQRLMIL
                     INDFVSRPINIDFIPEAWIEFLKEYLKTIDIKSYLTEENIEKIVSKILPGAWYIISGS
                     VAFVWNLVILIFILLYVIFISKDYETLSEGWINLIPKNYRHFALGMAEDVEKGMNSYF
                     RGQALIAFIVGILFAIGFKIINLPLGITFGLFVGVLNLVPYLQTISLIPALLLSIIKA
                     AEYNQNFLLVLLSVALVYVVVQAIQELFLNPNILSKATGLKPAIILLSLSIFGSLLGL
                     VGMIIALPSTSLIISYYKRFVIRREPITKRVDESFETDQSLETDNKPGTKAKAKPEKN
                     K"
     gene            4024..4347
                     /locus_tag="PKY68_05515"
     CDS             4024..4347
                     /locus_tag="PKY68_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOH24529.1"
                     /translation="MKRYGSVIKVRPEKLEEYKRLHAAVWPSVLKTIKDCNIRNYSIY
                     YKDGFLFSYYEYTGTDYQADMEKMAADPETQRWWTHCEPCQEPLETRAPGEWWAEMEE
                     VFHLD"
     assembly_gap    4413..4422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4447..5229)
                     /locus_tag="PKY68_05520"
     CDS             complement(4447..5229)
                     /locus_tag="PKY68_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOH24530.1"
                     /translation="MKESGKIVPTAGEKKSVTEEKGFTAGKKGFTAGSDHLLIRSFDF
                     TAHSLNITLTDFIEKLPSGENESEAFAKEIEQVIVDAARFLRPKAAYRIIDRASPEGD
                     LLKPKSSNSHLNALLNTELKKTEKIAVFACTAGAEIEGLYRSYLKRGESIKAFFADLL
                     GTIAVGKILELLYRELEKEYLNDGLHAGHAISPGDCGWPLKEQAVLFSLLPEDCCGIH
                     LNQSMMMIPQKSQSGIIPLGKGIRREKHRCSLCSSLNCPYRK"
BASE COUNT         1484 a         1126 c         1208 g         1479 t
ORIGIN      
        1 tatggcaaca acagcagatt ttagaaacgg catgtgcatc gagattgacg gtgcctatta
       61 ttctattgtg gaatttcttc atgtaaaacc gggaaaaggg gctgcttttg tccggaccaa
      121 gctgaaaaat gtacaaacgg gcagaatcct cgaaaaaact ttcaactctg gggtaaaagt
      181 agacgaagtc aggattgagc gtcgtcccta tcagttcctt taccaggacg atatgggcta
      241 taattttatg cattcggata ctttcgaaca aataaacata gctaaggaat cgatcaacgg
      301 agttgatttt cttaaagagg gagatatttg cgaagtggtg gtacacgccg aatcggaaac
      361 cgtgctttat gccgacacac ctgtacatgt tgaacttgaa gttacttata ctgagccggg
      421 cctcaaaggc gatacagcca ccaatgcttt aaaaccggcc accgtagaaa ccggagccga
      481 agtcagggta cctttgttta tcgaagtggg cgatcgcatc aaagtagata cccgcgacgg
      541 atcctatgtg gaaagagcaa aataacctgt tgttttttta ttttcaggcg cattatcaat
      601 ttcaagttaa tttgtattca actataaaaa gaaactgtcc ctgcgggcag tttttttatc
      661 ctttttgtat taataaatag tgaattatat attaaatttg ccggcattat gatagtttga
      721 aaaagcatgg agcaaaaaaa taagttcagt atacgcgatt ggtccgaaga agacaggccc
      781 agagaaaaaa tgatcaaaaa aggcgtagga agcctcagcg attcggagtt actggccatc
      841 atactgggtt cgggttctac aaaagaaagc gccgttgaat tggccaagcg catgttaaac
      901 agcgttgaca ataatctgag ccttttgagt aaaatgagtg tacaggaact cagatctgct
      961 tttcacggag tagggcaggc caaggccgta agtattctgg ctacttttga attgggtaag
     1021 cggaggcaac tggccgacat tcccgataaa gtgcagttaa acagcagtaa aaaaatctat
     1081 gagctgatgt accccctgat ggccgatctt aaacacgagg aatgttgggc tatttacatg
     1141 aattcttcca accgtctgat tgatatgaaa aagatcagtt ccgggggcat aaccgaaacc
     1201 accgtcgatc cgcgtattat attgaaacat gccatacagc atatggctac ggctctggtt
     1261 ctttgtcaca accatccttc gggaaatatt cagcccagcg atgccgacaa ccgtctgacc
     1321 aaacgtctga acgaagcctc caaaatgatg agcatttgta tgctcgacca tctggtgatt
     1381 tgcgacggca agtactttag ttatgccgat cagggtttat tgtgattctg agataattct
     1441 ttacctttgc ctcaaaaaaa atggacaata cgtttttgga gaaggaagag gccatcgtaa
     1501 aaatgttacg cacggtttac gatcccgaaa ttccggtaaa catctacgaa ttgggtttaa
     1561 tctacaaagt agatattgat gacgcttcca aagtgactat cgaaatgacc cttacttccc
     1621 cctcttgccc catggccgat tttataattg aagatgtgcg catgaaagtt cagtcgctcg
     1681 attttgtcgg cgatgtagat atcaatctgg ttttcgagcc ggaatggaac aaagacatga
     1741 tgtccgaaga ggccaagctc gaattgggtt atctttgatc gtatgagtac cgggggaaaa
     1801 atatactttg taaccgatgc tcatctgggt ttgcgcgccg acgtaccggt ggccgggaac
     1861 gaaaaaaaat tggtccgctg gcttgatagc attcaggccg attgcggagc cctgtatatg
     1921 ttgggcgata tgatagactt ctggtacgaa tacaaagagg ttgtgcctaa gggttttgtt
     1981 cgtttttttg gcaagctggc cgaatttacc gatgccggca ttcctgtgta ctggttcgca
     2041 ggcaaccacg atctttggtt gtttagctat gtccagcagg aaatgggcat tgttgtttac
     2101 aaggaagctc aggaactgga tattttgggc aagcgttttt atatagctca cggcgacggg
     2161 ctgggttatg cgccttttag cttcaggatc atgaatggga tattccacag ccctttctgt
     2221 caggccttgt tcaaaatttt acatcccgat ctgggcatta gtttcggact gaggtggtcc
     2281 aggcacagtt attataaacg caaaaccatg gaactggcct atctgggtga agacaaggaa
     2341 cacctgattc aatttgccaa aaaacacctg gccactgcaa aaccggcccc tgacttttat
     2401 gttttcggac atcgccacat cttgctcgat ttacaattaa cccgggattg ccgtctagtt
     2461 attcccggcg attggatcag ggaattcagc tacgccacct tcgacggtga ggatcttgtg
     2521 ctcgaccatt tcgaagaata aactccaaaa actaacaagt cgtttatttg tttttttccg
     2581 gcttagcctt tgcctttgtt cccggtttat tgtccgtttc gaggctttga tccgtttcaa
     2641 agctttcatc cactctcttg gtgataggtt cccgtcttat gacaaaacgt ttgtagtaag
     2701 aaataatcaa ggaagtgctg ggaagcgcaa taatcatccc aaccagcccc agcaggctgc
     2761 caaatatgga cagcgaaagc aatatgatgg ccggcttgag cccggtggcc ttactcagga
     2821 tattaggatt gagaaacagt tcctgtatgg cctgtaccac cacataaacc agggcaacac
     2881 tgaggagtac cagcaaaaag ttttgattgt attcggcggc ttttataatg ctcagcagta
     2941 gagccggaat cagggaaatg gtctgcagat agggtaccag gttgagtact cctacaaaca
     3001 gaccaaaagt aatacccaaa ggtaaattaa tgatcttgaa tcctatagca aacaaaatgc
     3061 ctacaataaa ggcaatcaga gcttgtcctc tgaaatagga attcattccc ttttccacgt
     3121 cctctgccat acccagggca aaatgcctgt aattcttggg tatcagattg atccaacctt
     3181 ccgaaagtgt ttcgtagtct ttggagatga aaatcacgta cagcaagata aatatcaaaa
     3241 tcactaaatt ccatacaaaa gccactgaac cggaaataat gtaccaggct ccgggtaata
     3301 tttttgatac gattttttca atattttctt ccgtcagata gcttttaatg tctatggttt
     3361 tgaggtattc tttcaggaat tctatccagg cttcgggaat aaaatcgata ttaatgggtc
     3421 gactcacaaa gtcgttgatc aaaatcatta agcgttgaat ttcgtttaag accggcgttt
     3481 tcagaaagtg gattatcata cccaacaaac caaaaatcaa caacagagta accactatgg
     3541 aaagcacacg gtttttcagc ttgagcttat gctgaaaaaa caagacggcc ggacgtataa
     3601 aataagccag gaaccaggcc accaaaaaag gtaaaagtac cgcactcagt cgataaataa
     3661 gcaatcccag tccggttatg accaaaaggg cgatcactat gcgaacaacg cgatcgaagg
     3721 tgtagggttt attttggttt tccatttcta tgcgatatta tgtttgccgt ttgaatactg
     3781 ttgtcttgtc cggccctttt tttgaccccg gaaatgtttt gactccggaa agtttcgacc
     3841 tcagaatcgt ttcaaccctg ggaatgtttc gaccccggga tggaaacaaa agtagagaat
     3901 agatttgaat tgaagcctca agttttacca taatttttgc tttttgtccg gtctttgttc
     3961 ctttggaagc tttgcagatt taaaacgaat gccctatttt tgcataaatt aatcctgaaa
     4021 attatgaaac gttacggcag tgtgatcaaa gtaagacccg aaaaactcga agaatacaaa
     4081 cgcctgcatg cggcagtctg gccttcggtg ctcaaaacca tcaaggattg caacattcga
     4141 aattattcca tctattacaa agatggcttc ttgttcagct attacgaata taccggcaca
     4201 gattatcagg ccgatatgga aaaaatggct gccgaccccg aaacacaacg ttggtggacg
     4261 cattgcgagc cttgccagga accgctcgaa accagggctc cgggtgaatg gtgggctgag
     4321 atggaagaag ttttccacct cgattaagca atcttataga ttaagcaacc tcgttgacca
     4381 agcaacttta ttgttggtga ccttcccatg aannnnnnnn nngagggttt atgccaaagg
     4441 gcaaaactat ttgcggtaag ggcagttgag cgagctacag agggagcacc ggtgtttttc
     4501 ccttcttatt cctttaccta agggaatgat gccgctctgt gatttttggg gtatcatcat
     4561 catggattga ttcaggtgta tgccacaaca gtcctcaggc aggagactga acaggacggc
     4621 ttgttctttc aatggccaac cgcaatcgcc cgggctgatg gcatgacctg catgtaaacc
     4681 gtcgttgagg tactcttttt ccagttcccg gtaaaggagc tccaggattt ttcccacggc
     4741 aatagtgccc aacagatcgg caaaaaaggc cttgatgctt tctccccgtt tcaaatacga
     4801 tctatagagt ccttcgattt ctgccccggc tgtgcaggca aaaacggcta ttttttccgt
     4861 ttttttcagt tctgtgttta ataaagcatt taaatgcgaa tttgagcttt tcggcttcag
     4921 taaatctcct tccggggagg cccggtctat aatccggtac gctgcttttg gtcgcaagaa
     4981 tcttgcggca tcgacgatca cctgttcaat ttccttagca aaggcttccg attcgttttc
     5041 gccggagggg agcttttcga tgaaatcggt caaagtaata ttcagtgagt gggcagtaaa
     5101 gtcaaagctg cggattaaaa gatgatcgct tccggctgtg aatccttttt ttccggctgt
     5161 gaatcctttt tcttcggtca cggatttttt ttctccggct gtgggtacaa tttttccaga
     5221 ctccttcatt gcttaaaaat ccatggagag tgaaacattc aactgtctgc cggtcaggta
     5281 attgggtatg gcccattctt gtcctgc
//
LOCUS       DAOSGL010000145         5288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13846_length_5288_cov_4.065354, whole genome shotgun sequence.
ACCESSION   DAOSGL010000145 DAOSGL010000000
VERSION     DAOSGL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13846_length_5288_cov_4.065354"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..909
                     /locus_tag="PKY68_05525"
     CDS             160..909
                     /locus_tag="PKY68_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOH24531.1"
                     /translation="MKSDSVVIIPTYNEKENIEAIIREVFDLPQPFHILVVDDGSPDG
                     TASIVKKLQKEFPSALHLEERKGKLGLGTAYIHGFKWALRNGYDYIFEMDADFSHNPK
                     DLLKLYQACSKDGADLAIGSRYINRLVNVVNWPIGRVLMSYYASVYVRIVTGMKVSDT
                     TAGFKCYRRRVLETIELDNIRFKGYAFQIEMKFTTWKHGFRIVEIPIVFTNRELGTSK
                     MSGGIFNEAVFGVIKMRLKSVFKKYPQINRN"
     gene            910..2259
                     /locus_tag="PKY68_05530"
     CDS             910..2259
                     /locus_tag="PKY68_05530"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOH24532.1"
                     /translation="MQKCLIKNATLVNEGKIFKASVLIEGQHISRIFTEEAVIPSEVL
                     EKYPVIRAEGCYLLPGVIDTHVHFREPGLTHKADMLSESCAALAGGVTSFFDMPNTIP
                     QTITLDAWEQKMSLAAEHSMANYAFYLGATNGNLSELIHADYSRIPAVKLFMGASTGN
                     MLVDESSALKELFSSIKAIIAVHAESENIIRQNKERYMAQAGIDLPIRYHSLIRDAEA
                     CYRSSAQAVEWASKYGSRLHILHLSTQRELSLLKDLALEEKKVTAEVCVHHMWFDDRD
                     YERLGAAIKWNPSVKQETDKLALRTALNSNLIDVVATDHSPHLWFEKEGSCFEAASGA
                     PSVQFGLVLMLELSGRGAFGLDTVVEKMCHAPARLFRIVKRGFIREGYFADLTLLKPG
                     AARKIGKGEIISKCGWSPYEGENVSWHVEKTFVNGSLLYNQGRFSQEKAARALEFSV"
     gene            2439..5159
                     /gene="ppdK"
                     /locus_tag="PKY68_05535"
     CDS             2439..5159
                     /gene="ppdK"
                     /locus_tag="PKY68_05535"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOH24533.1"
                     /translation="MSKKSVYFFGNKTAEGNGNMRELLGGKGANLAEMNLLGIPVPPG
                     FTISTEICTEYFKKGRDKVVAEIKAEVEAAMKKVEKAVNGPGFGDPENPLLVSVRSGS
                     RASMPGMMDTILNLGMNDQAVEVLSKRSGNPRFAWDSYRRFVQMYGDVVLGMKPEKKE
                     DEDPFEVIIHKMKSQRGVSNDTDLTTDDLKELVSLFKAAVKQQTGQEFPADPWVQLWG
                     AVCSVFDSWMNERAILYRKLNNIPSDWGTAVNVQAMVFGNMGDNSATGVAFTRDAATG
                     EDMFNGEYLINAQGEDVVAGIRTPQQITLEGSKRWAQAQNIDEQERQTKYPSLEETMP
                     EAYKELYAIQEQLEVHTKDMQDIEFTIQDGKLWMLQTRSGKRTGAAMVKIAMDMLRDG
                     MLTEKEALLRVDPEKLDELLHPVFQKAALDAAKPISKGLPASPGAASGRVVFFAEDAE
                     EWADKGEKVILVRTETSPEDLKGMNKAQGILTARGGMTSHAAVVARGMGKCCVSAANN
                     LFIDYKARNMKVGDKSIKEGDWLSLNGSTGYVYEGKLATQDPELSGDFAQLMKLSDKH
                     TRMQVRTNADTPHDAAVARSFGAVGIGLTRTEHMFFEGDKIIAMREMILAGDEAGRRK
                     ALAKLLPLQKADFQGIFEVMHDLPVTVRLLDPPLHEFVPHDEKGQKEMADVMGMTLAQ
                     VKERVESLVEQNPMLGHRGCRLGNTYPEITEMQTRAIIEAALDLKAKGIKVLPEIMVP
                     LTGNYKEFNAQKAVIDETAEAIFLERNARIEYKVGTMIEIPRAALTANRIAESAEFFS
                     FGTNDLTQMTFGYSRDDAASFLPIYLEKGILENDPFAILDQKGVGKLVDMATQKGRDT
                     NPNLKCGICGEHGGEPSSVEFCHRVGLNYVSCSPYRVPIARLAAAQAALR"
BASE COUNT         1520 a         1124 c         1301 g         1343 t
ORIGIN      
        1 agtcctgtag ttccaatttg ggaattaaaa tcttgcaaaa ttacaaaaaa tacggtcaat
       61 gcagcaggaa gaagcaaaaa aaccgtatat ttgggggttt atttgtcgag cctaataaag
      121 taatattaat cagcagaaat taaacacaaa tttcgtacaa tgaaatcaga ttcagtcgtc
      181 attattccca cctacaacga aaaagaaaac attgaagcca tcatacggga agtttttgat
      241 ttacctcagc cctttcatat tttggttgtc gatgacggtt ctcccgatgg tacggcttcc
      301 attgtaaaaa aattgcagaa ggaatttcct tcagctcttc atctggaaga gagaaaagga
      361 aagcttggcc tggggacggc ttatattcat ggctttaaat gggctttgcg caacggctac
      421 gattatattt ttgaaatgga tgccgatttt tcacacaatc ccaaggattt actcaaatta
      481 taccaagcct gctccaaaga cggagcggat ttggctatcg gctcacgcta tataaacagg
      541 ttggtaaatg tggtcaactg gcctatagga agggtgttga tgtcgtacta tgcctccgta
      601 tacgtacgca tcgttaccgg aatgaaggtg tccgatacca cggccggttt taaatgctat
      661 cgtcgccggg tactagaaac cattgaactc gataacattc gtttcaaagg ctatgctttc
      721 cagatagaaa tgaagtttac gacctggaaa cacggatttc gtattgttga gattcccata
      781 gtgtttacca accgggagct cggcacttct aaaatgagtg gtggtatatt caacgaagct
      841 gtttttggtg taatcaaaat gcgactgaaa agtgttttta aaaaataccc tcaaataaac
      901 aggaactaaa tgcaaaagtg cttgattaag aatgccaccc tggtcaacga gggtaaaata
      961 ttcaaggcat ccgttttaat cgaaggacaa cacataagca ggatatttac cgaagaggcc
     1021 gtcatacctt ctgaggtact ggaaaaatat cccgtcatac gggcagaggg ctgttattta
     1081 ttgccgggcg tcatagacac tcatgtacat tttcgggaac cgggactcac ccacaaagcc
     1141 gacatgttgt cggagtcgtg cgccgcgttg gcgggagggg ttacgtcttt ttttgatatg
     1201 cccaacacca tcccccagac aatcactctg gatgcctggg aacaaaaaat gagtttggcc
     1261 gcggaacatt ccatggccaa ttacgcattt taccttggtg ccaccaatgg taatttatct
     1321 gaacttattc atgcagatta cagccgcatt cccgcagtga agctttttat gggagcttcg
     1381 accggtaata tgctggtcga tgaatcttcg gcgttaaaag agctcttttc aagcattaaa
     1441 gcaataattg ccgtccacgc cgaatcggag aatattatca gacaaaacaa agaacgctat
     1501 atggctcagg cgggaatcga tcttcccatt cgttaccatt cactgataag ggatgccgaa
     1561 gcttgttacc gttcttctgc tcaggccgta gaatgggcaa gcaaatacgg cagccgcctg
     1621 catattttac acctttctac ccaacgtgag ctcagcttgc tcaaggacct ggccctggaa
     1681 gaaaaaaagg ttaccgctga agtctgcgtg catcatatgt ggttcgacga ccgggattat
     1741 gaacgactgg gtgctgccat caagtggaat ccttccgtta aacaggagac ggacaagctg
     1801 gcccttagaa ctgccttaaa cagcaatttg attgatgtgg tggccacaga tcattccccg
     1861 catctctggt tcgaaaaaga aggttcctgc tttgaagcgg catcgggagc tccttctgtt
     1921 cagtttgggt tagtcttgat gctggagctt tccggcagag gtgctttcgg cctggatact
     1981 gtggttgaaa agatgtgtca tgcgccggcc cgcttgtttc gaattgttaa gcgaggtttt
     2041 attcgcgaag gttattttgc cgatttgact ctgcttaaac ccggtgcagc ccgcaaaatc
     2101 ggcaaagggg aaatcatcag taaatgcggg tggtcgcctt atgaggggga aaatgtaagc
     2161 tggcatgtgg agaaaacttt tgtaaacggt tccctgctgt ataaccaggg tcgttttagt
     2221 caggaaaaag cggcccgagc cttggaattt agtgtctgag acatagaccg gatgttgaaa
     2281 caaacaagtt ttttggtaca tgggctgtat ttatatcagg gtcaatttgg gtttttgttt
     2341 gttcatatct aaagaaacat ttaactttgc ggcctgatag atactaaaat agagctgtta
     2401 cagctataac aactactaat ttttacaata attttacaat gagcaagaag agcgtttatt
     2461 tttttggaaa taaaacagcc gaaggcaacg gaaatatgcg cgagctgtta ggaggtaaag
     2521 gtgccaacct ggccgagatg aatttgctgg gtatacctgt tcctccggga tttaccatta
     2581 gtaccgaaat atgtaccgag tatttcaaaa aaggcagaga taaagtagta gccgaaataa
     2641 aagcggaggt tgaagcggcc atgaaaaaag tggaaaaagc tgtaaacggc cccggctttg
     2701 gagatcccga aaatcctctg ttggtttcag tacgctccgg atccagagct tccatgcccg
     2761 ggatgatgga taccatcctg aatctgggaa tgaacgacca ggctgtggaa gtcttgtcaa
     2821 aaagatcagg caatcctcgt tttgcctggg attcttaccg caggtttgta caaatgtacg
     2881 gcgatgtggt gttgggtatg aaacccgaaa agaaagaaga cgaggatcct tttgaagtga
     2941 ttattcataa gatgaaatcc caacgcgggg tttccaacga taccgatctc actaccgacg
     3001 acctcaaaga actggtttct ttgtttaagg ccgccgtaaa gcaacagacg ggtcaggaat
     3061 ttcccgcaga cccctgggtg caactttggg gtgccgtctg ttcagtgttt gacagttgga
     3121 tgaacgaacg ggccatcttg tatcgcaagc ttaataacat tccttcagat tggggaactg
     3181 ccgtaaacgt gcaagccatg gtttttggca atatgggcga taactcagcc accggtgtgg
     3241 cttttaccag agacgcagcc accggcgaag atatgtttaa cggcgaatat ctcatcaatg
     3301 cacagggcga ggacgtggta gccggaattc gcaccccaca gcaaatcacc ctcgaaggtt
     3361 ccaagcgctg ggcacaagct cagaatattg atgaacagga acgccaaacc aagtatcctt
     3421 cactggaaga aacaatgccc gaagcctaca aagagctgta tgccattcag gaacagttgg
     3481 aagtccatac taaggacatg caggacattg aatttaccat acaagacggt aaattatgga
     3541 tgctgcagac acgtagcggt aaacgtaccg gagctgctat ggtaaagatc gccatggata
     3601 tgctgcgtga cggtatgttg acagaaaaag aggctctttt gcgggtagat cccgaaaaac
     3661 tggacgaact tttgcatcct gttttccaaa aagcagcact ggatgccgcc aagccgatat
     3721 cgaaaggttt gccggcctca ccgggagcag cttcgggccg tgtggtcttt tttgcagaag
     3781 acgccgaaga atgggcagac aaaggagaaa aggttatttt ggttcgcaca gaaacctcgc
     3841 ccgaagacct gaaaggaatg aataaagctc agggcatttt aacagcccgt ggcggcatga
     3901 cttctcatgc ggctgtggta gcccgtggga tgggtaaatg ctgtgtatca gccgccaata
     3961 acctgtttat tgactacaaa gcccgcaata tgaaagtggg cgataaaagc ataaaagagg
     4021 gagattggtt gtcgttaaac ggatctaccg gttatgttta cgaaggcaaa ctggctaccc
     4081 aggatcctga actgagcggt gattttgctc agttaatgaa gctaagcgac aaacatacac
     4141 gcatgcaggt aagaaccaat gccgacaccc cgcacgatgc agctgtggcc agatcttttg
     4201 gggccgttgg tatagggctt acccgtacgg aacatatgtt ttttgagggc gacaaaatca
     4261 tagccatgcg cgaaatgatt ctcgccggtg acgaagcagg cagacgtaaa gcgctggcca
     4321 aactcttgcc tttacaaaaa gccgactttc aaggtatttt cgaagtgatg cacgatttgc
     4381 ccgtaactgt ccgtctgttg gatccccccc tgcacgaatt tgttcctcac gacgagaaag
     4441 gacaaaaaga aatggctgat gttatgggta tgaccttggc tcaagtcaaa gaacgtgttg
     4501 aatcgttggt agaacaaaat cccatgttgg gtcacagagg ttgccgtttg ggtaatacgt
     4561 atcctgaaat taccgaaatg caaacccggg ccattattga agctgcgctg gatctcaaag
     4621 caaaaggcat taaggtcctt cccgaaatca tggtgcctct tactggtaac tacaaggaat
     4681 tcaatgctca aaaagccgtt atagacgaaa cagcagaagc cattttcctg gaacgcaatg
     4741 cacgcataga gtacaaagtt ggtaccatga tcgaaattcc gagggctgct ttaacagcta
     4801 atcgcattgc cgagtcggca gaattctttt catttggaac caacgacctg actcaaatga
     4861 cctttgggta tagtcgcgac gatgcggctt ctttccttcc catttatttg gaaaaaggaa
     4921 tattggaaaa cgaccccttt gccatactgg atcagaaagg ggtaggaaaa ttggtcgata
     4981 tggcaactca aaaaggaaga gacacaaatc ccaatctgaa atgtggaata tgcggtgagc
     5041 atggtggaga gccctcgtcg gtggaatttt gccatcgtgt gggattgaac tatgtaagtt
     5101 gctctcccta tagggtgcct attgcccgtt tggctgctgc gcaggccgct ctccgctaag
     5161 gaacggtttc tgttcagtac gagaatttga tttgacaaaa atcgataaaa tactatgaca
     5221 aacaaagaat ttattcgtgc tgttcagctt gaatccaaac tggataaaca aaaaacagct
     5281 ttgctggt
//
LOCUS       DAOSGL010000146         5261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_13947_length_5261_cov_3.779677, whole genome shotgun sequence.
ACCESSION   DAOSGL010000146 DAOSGL010000000
VERSION     DAOSGL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13947_length_5261_cov_3.779677"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..796
                     /locus_tag="PKY68_05540"
     CDS             227..796
                     /locus_tag="PKY68_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOH24534.1"
                     /translation="MKKVVSKGIGGYSFQIEEDACRILQDYIEQFKSKLGEAEDVSEI
                     MDDIEARIAEILREEIHSPNQVVGEGLIRQVVARMGMPDGSNDFTEYHVPYTSTKRAT
                     RRLFRNPDDRKIGGICGGLGAYLGVDATLLRILFLIGFFLGLLSFWVYLILWICVPDA
                     RTPAQKLQMQGLPVTAENLLRYSAKQSEK"
     gene            805..1140
                     /locus_tag="PKY68_05545"
     CDS             805..1140
                     /locus_tag="PKY68_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOH24535.1"
                     /translation="MNTDNVKAQMRKGVLEYSILSILSRGDEYASSLIKILKDARMIV
                     VEGTLYPLLTRQKNQGLLSYRWEESPQGPPRKYYSITDKGREMLSELDVTWKEIVDSI
                     EIIRNTPNQ"
     gene            1137..2672
                     /locus_tag="PKY68_05550"
     CDS             1137..2672
                     /locus_tag="PKY68_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOH24536.1"
                     /translation="MKNVIKVSIGRWAFTLNEEAYILLGNFLDRLKAYYEKEADTDKR
                     VQEIENLIGEYLSDCVKSIDQVVDEKQIQQAIESLDLPDFGQQAASTDGSAAESDNYG
                     RSQKRLYRSAERKVLGGVFSGLGEYFGIDPVGLRLLYVVFVVIGMFSFRVNMGDHVHF
                     RLFGIGGITSFLLLMYLIMWIVMPLARTPQEKRAMRGGRTSARRVVHDLGTQPGRNYR
                     EDRNFTLGSFLLNSIRVFVGVVLILVGASGLLALPMLLYFWDMPADWFGYLELLPLRP
                     GLLWFKVLTLIAVFVPLLLFLYEGIVLLLKLRVRKFNLGAIFLVVWILSLFILAFSGL
                     LASRSFWGEGKYQSTVTLDLPSDTLYLEIDAEFSSPEQFVCAYEDEFFVYCNKSSSDD
                     KFLYIIPQVRLSSGDTPKMTQGVSLRAKNNIIAQIKAEDCVDFVKQEGNKIKVSPLYF
                     DKDKPWTFEQMSLHLFLPENAVLYVQGKKGKWQEIRHNRGSFYPFVGYTDDIEGFIIS
                     NSF"
     gene            complement(2791..3369)
                     /gene="purN"
                     /locus_tag="PKY68_05555"
     CDS             complement(2791..3369)
                     /gene="purN"
                     /locus_tag="PKY68_05555"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOH24537.1"
                     /translation="MKNLQKHRLAVFASGSGSNAENIARYFDGREDVKVVLFLSNKKN
                     AYVLERGKKLRIASHAFEKTDMEEGRILQLLQENQVNFIVLAGYLQKIPPEIIQAYPD
                     KIVNIHPALLPKFGGKGMYGERVHEAVIQAGEKESGITIHYVNEHYDRGNIIFQASCP
                     VLASDTPSELALRVHELEYLHYPRIIDKLLKE"
     gene            3522..3761
                     /locus_tag="PKY68_05560"
     CDS             3522..3761
                     /locus_tag="PKY68_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH24538.1"
                     /translation="MSETADKVKSIIAEKLGIELSQVTPTASFTNDLGADSLDTVELI
                     MSFESEFGISIPDEEAEKISTVSDAIDYVEKTLKK"
     gene            3830..5093
                     /gene="fabF"
                     /locus_tag="PKY68_05565"
                     /pseudo
     CDS             3830..5093
                     /gene="fabF"
                     /locus_tag="PKY68_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224505.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
BASE COUNT         1419 a         1061 c         1257 g         1524 t
ORIGIN      
        1 atggtgtaat ggtagcactg caggttttgg ttctgcctgt cgaagttcga gtcttcgtga
       61 gacaaccaaa aagcctccgg ttcccaaacg ggccggaggc tttctaattg taaaataatc
      121 ttaaatctca gtactatgta ttgcatagta ctatacacca tactatattt gcccccgaat
      181 accggattcc gatgttggaa ccggactaaa tcaaataata aaactcatga aaaaggttgt
      241 ttcaaagggg attgggggct attctttcca aattgaagaa gatgcctgcc gcattttgca
      301 ggattacatt gaacaattta agagcaagct gggagaagcc gaagacgtct ccgaaatcat
      361 ggacgatatt gaggcccgca ttgccgaaat tcttcgggaa gaaatacatt ctcccaacca
      421 agttgtgggc gaaggcctga tacgacaagt agtggcacgc atgggtatgc ccgacggcag
      481 caatgatttt acagaatatc acgtgcccta tacttcgaca aaaagagcca cacgcaggct
      541 tttccgtaat cccgacgacc gtaagatagg tggaatatgc ggaggtttgg gagcctattt
      601 gggagtggac gccaccctgt tgaggatttt gtttttgatt ggcttctttt tggggctctt
      661 gtcgttttgg gtatacctta ttttgtggat atgtgtgccg gacgcaagga cacctgcaca
      721 aaaactgcag atgcagggac ttccggtaac ggccgaaaat ttattgcgtt attcagctaa
      781 acaatcagag aaatgaaccg ggaaataaat acggacaatg ttaaggctca gatgagaaag
      841 ggagttctgg agtatagtat tctatccatc ttgtccagag gagatgaata cgcttcttcc
      901 ctgatcaaaa ttcttaaaga cgcccggatg atagtggtgg agggcacttt gtaccccctg
      961 cttaccagac aaaagaacca gggtttgctc agttaccgtt gggaagaatc gcctcagggc
     1021 cccccccgca agtattacag tatcacggat aagggcaggg aaatgctctc ggaacttgat
     1081 gtgacctgga aggaaattgt cgatagtata gaaataattc gtaatacccc aaatcaatga
     1141 aaaatgtaat taaagtaagt attggtcgct gggcctttac cttgaacgaa gaggcctata
     1201 ttttgctggg aaactttctg gaccggctca aggcttatta tgaaaaagaa gccgatacgg
     1261 acaaacgcgt ccaggagatt gaaaacctta tcggggagta tttatcggat tgcgttaaat
     1321 ctatcgatca agtggtggac gagaaacaaa tacagcaagc catcgagtcg ctggatttac
     1381 ccgattttgg acaacaggct gcttcgacag acggcagcgc ggcagagagc gataattacg
     1441 gcaggagtca aaaacgcttg tatcgtagtg ctgaacgtaa ggtgctgggc ggagtgttca
     1501 gcggactggg cgaatatttt ggtatagatc cggttggctt acgtctcctg tacgtggttt
     1561 ttgttgtgat cggtatgttt agtttccgcg ttaatatggg cgatcatgtc catttccgcc
     1621 tgtttggaat aggaggcatt acttcttttc ttcttttgat gtatttgatt atgtggatcg
     1681 taatgccttt ggctcgaacg ccccaggaga aaagagccat gaggggagga cgaacttcgg
     1741 cccgcagggt tgttcatgat ttagggactc agcccggcag aaattaccgc gaggacagaa
     1801 atttcactct gggcagtttt ttgctcaact ctatccgtgt ttttgtaggt gttgttttaa
     1861 ttttggttgg tgcttccggt ttgctggctt tgcccatgtt gttgtatttc tgggatatgc
     1921 ctgccgattg gtttggttat ctggagttat tgcctctgcg tccgggtttg ctctggttca
     1981 aggtattgac attaattgcc gtttttgtgc ctttgttgtt gttcttgtac gaaggaatag
     2041 ttctgttgct taaactgcgg gtgcgtaaat ttaacttggg agctattttt cttgtcgttt
     2101 ggatattatc tctttttata cttgctttca gcggtttact tgccagccgt tctttttggg
     2161 gtgagggaaa atatcagagc acagtaaccc tggatttgcc ctcagatacc ttataccttg
     2221 agatagatgc cgaattttct tcgcctgaac aatttgtatg tgcttacgaa gatgaattct
     2281 ttgtttattg taacaaaagc agctccgacg acaaatttct ctatattatt cctcaagttc
     2341 gcctgagcag cggtgatacg cccaaaatga cccaaggggt gagtttaagg gctaaaaaca
     2401 acattattgc ccaaattaag gccgaagact gcgttgattt tgtgaaacag gaaggaaaca
     2461 aaattaaagt cagtccttta tattttgaca aagataaacc ctggactttt gaacaaatga
     2521 gcctgcattt gtttttaccc gaaaatgctg tactgtatgt acaggggaaa aagggtaaat
     2581 ggcaggaaat aaggcataac agaggttcgt tctatccttt tgtgggctat accgacgata
     2641 ttgaaggctt tataattagt aattcatttt aagtaaaaca gcatcaatcg gatcgactta
     2701 tcaatcctcc ggaaccagcc catgcttccg gaggattttt tagataatgc ccattcaaga
     2761 gataccaatt atcatataaa gatgcaatga ttattctttc aatagtttgt ctataatcct
     2821 ggggtagtga aggtattcca gttcgtgtac gcgcagtgca agctccgaag gcgtatccga
     2881 agccaacacc ggacaactgg cttggaaaat gatgtttccc cgatcgtagt gctcatttac
     2941 gtagtggatg gtaatgccgg attccttctc gcctgcctgt ataactgctt cgtgtacccg
     3001 ttcgccatac atgcccttgc ccccgaattt gggtaataat gcggggtgaa tgtttactat
     3061 tttgtcggga taagcctgaa taatttcagg cggaattttt tgaagatagc cggccagaac
     3121 aataaaattt acttgatttt cctgcaacag ctgcaggatt cttccctctt ccatgtctgt
     3181 tttttcaaaa gcatgggagg caatacggag ttttttcccc ctttcgagta cgtaggcatt
     3241 ctttttgttg gaaaggaata atacgacttt cacatcttcc cttccatcga aatagcgggc
     3301 tatgttttcg gcgttagagc cggaaccgga ggcaaaaacg gccaggcgat gcttttggag
     3361 gtttttcata attgacagat attcaacaat atgttaaaaa gcgaaaggag tttttagaat
     3421 aattacagca caaaattagc gtttgtttga aatatttcat tcctttgtcg tctcaatttt
     3481 ttttgagaca aacttattta ttcactaaaa aattaactgt tatgtctgaa actgcagata
     3541 aagttaaatc gattattgcg gaaaaattag gcatcgaact ttctcaagtt acccctactg
     3601 caagcttcac taatgatctt ggtgctgact ctctcgacac tgtagagttg attatgagtt
     3661 ttgaatcgga attcggcatt agtattcctg acgaggaagc tgaaaagatc tcaacagtca
     3721 gtgacgcaat cgattacgtt gaaaaaactc tgaaaaaata attccataca ggagttcccg
     3781 gtacataaga ggaaaaccga acacgggact tcttgaagat tcctagaaaa tggaattaaa
     3841 aagagttgtc gtaacaggcc ttggagctat tacccctttg ggtaattccg tatcagaata
     3901 ttggacgggt ttacttaatg gagtaagtgg ttccgggcct atttcacgtt ttgacactac
     3961 acatttcaaa acaaaatttg cctgtgaggt aaagaattat aatccggaag attatttcga
     4021 tcgcaaagag attcgcgtgt acgaccattt tgcacagtac gccataattt ctgcaaagga
     4081 agctatttcc gatgctcgct taattcccga gcaagaagat ttggacagaa ttggtgttat
     4141 ctggggcgcc ggaataggag gtattcaaag ttttgaaaat gaggtgactg attttgccaa
     4201 aggagacggg aaacccaggt ttagcccgtt ttttgttccc cggatgattg cagatttgtc
     4261 ggcaggtcat atttcgatca tgtatggctt ccgggggccg aattattgca ccgtatcggc
     4321 atgcgcctct tcgaataacg ccttttttga tgctttcaat tacatacgtt tgggtaaagc
     4381 caatgttatt gtttgcggag gttccgaggc tgccattact ccctgtgggg taggtggatt
     4441 caatgccatg cgtgccattt ctacccgcaa cgaagattac gccactgcca gccgtccctt
     4501 cagcaaaagc cgcgatggtt ttgtgctggg cgagggagga gcttgcctga ttttcgaaga
     4561 gctggaacat gcaatggccc gtaaggctcc tatttacgcc gaggtggccg gttgtggatt
     4621 atcggccgat gctcaccaca ttacggcacc tcatcccgat ggcctgggtg ccaccaaagt
     4681 aatggaaaat gcgctggagg atgccggaat gcagcccgaa gacatcgact acatcaatgt
     4741 gcacggaacc tccactccct taggtgatat cgccgaagtg aaggctgtca aaaaaatatt
     4801 cggtgaacac gcatataaat tgaacatcag ttccaccaaa tcaatgaccg gacacctgct
     4861 gggtgcggcc ggagcgatag aatctgttgc ctgcatcaag gctacaggaa aacatcattc
     4921 ctcccaccat caattttacc gaaggagacg aagatcccga aatcgattac ggcattgatt
     4981 ttacctttgg aaaagcctgt aaacgcgagg ttcgcgctgc tttgtccaat accttcggat
     5041 ttgggggaca taatgccagc attattttca aaaagtggga cccgagcgta taacttttcc
     5101 gttttttttc aacaaggcat tttataaaaa ggaaaacagg gaatcgtatt ctgtttttag
     5161 aaagattctc gggttttatc cccgtgatct ggctttgtat gaattggctt ttcgacacaa
     5221 atcggcctcg ataaggggac gcgatggcgt tttactcaat a
//
LOCUS       DAOSGL010000147         5240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14019_length_5240_cov_3.459981, whole genome shotgun sequence.
ACCESSION   DAOSGL010000147 DAOSGL010000000
VERSION     DAOSGL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14019_length_5240_cov_3.459981"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKY68_05570"
     CDS             complement(<1..637)
                     /locus_tag="PKY68_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOH24539.1"
                     /translation="MKIRIGFFVLITFLSTVLYAQVTRVCFNEVLINNKTNFIDEYGQ
                     RSAWFELFNASYGIVDIGGCYLSNDRNNPTMYMIPKGDVLTKIQQRQHVLFYADGTPH
                     KGNFYVNFTLDTASVNVLYFFNADGKSLIDSVVVPILPADLSYARTIDGEENWEIMRR
                     VTPSTNNLTLDRNEKVEKFAKNDPYGIGMTLSAISVVFSVLVLLSLFFYWFA"
     gene            complement(640..2193)
                     /locus_tag="PKY68_05575"
     CDS             complement(640..2193)
                     /locus_tag="PKY68_05575"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOH24540.1"
                     /translation="MSNQLEKVKELIAFREQARLGGGIKAIEKQHAKGKYTARERINM
                     LLDEGSFEEFDMFVKHRCTNFDQDKKHFLGDGVVTGYGTIEGRLVYVFAQDFTVFGGS
                     LSETMSLKICKVMDQAMKMGAPVIGINDSGGARIQEGINALAGYGEIFERNILASGVI
                     PQISGIFGPCAGGAVYSPALTDFTLMTKGTSYMFLTGPKVVKTVTGEDVTQEELGGAS
                     IHATKSGVTHFAVDNEEEGIALIRKLLGYIPQNNLEEAPMKDCTDPIDRLEDSLNDII
                     PDSPNKPYDMYEVIGAVVDNGEFLEVHKDYAKNIIIGFARFNGQSVGIVANQPKMLAG
                     VLDCNASRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYGGVIIHGAKLLYAYGEA
                     TVPKVTVTLRKSYGGAHIVMSCKQLRGDINYAWPTAEIAVMGASGAVEVLYAKEVAAA
                     EDPAKLVAEKEAEYSEAFQNPYKAAQYGYIDDVIEPRNTRFRVIRALQQLQTKKLTNP
                     AKKHGNIPL"
     gene            complement(2206..2616)
                     /gene="mce"
                     /locus_tag="PKY68_05580"
     CDS             complement(2206..2616)
                     /gene="mce"
                     /locus_tag="PKY68_05580"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOH24541.1"
                     /translation="MKPTHIEHLGIAVKSLETAIPYVENIFGLKCYAIEEVPEQKVKT
                     AFFKVGQTKIELLESTDPEGPIGKFIENRGEGVHHIAFAVENLQEKLDTVAEKGVRLI
                     DKTPRKGAEGLNIAFLHPKFTLGVLTELCENPNK"
     gene            complement(2709..3743)
                     /locus_tag="PKY68_05585"
     CDS             complement(2709..3743)
                     /locus_tag="PKY68_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOH24542.1"
                     /translation="MNNNSGIIDALLNGIADAQDSHSNIQTIYTDAEATFLKNNALFF
                     IKPEITRADPAIRLKEVLTLILDKLAAFELRIHSIRILPAAYLKKHDLIRSHYGVISR
                     VASEGAKTLGDEAKGRFREVFGLEPSEARILGGLEVLETYPFFNSRSLDCLWQNGQNH
                     KLAGGTYAEKWRIDSDTVYVLNAFHPRQLEHFTQPGRSIVVMNISGDTDWSDLRNSFA
                     GATNPQKAMPGSLRNDLCLRARELGLGEISQSQNGVHLSAGPVEALLELQRFESDLDR
                     DKALLPFETFPFGRQLLTVFEQIPAKILNNEKINHQGKQVSIFDLTEEMNATEALSFL
                     KGLAETGIFM"
     gene            complement(3754..4224)
                     /locus_tag="PKY68_05590"
     CDS             complement(3754..4224)
                     /locus_tag="PKY68_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOH24543.1"
                     /translation="MKYLTELNSLLPLLGHRNWILVVDKAYPLQSSKGISYLNSGEDL
                     NKVLKEVLNALEAASHLRPIIYLDKELEFMDDELCKGVSDFKTTLQRICSNYTLKQIP
                     HEDIFRRLDEAARLFNVVVIKTESQIPYTSVFIELDCGYWSEEKEKTLRNKIVL"
     gene            4431..>5240
                     /locus_tag="PKY68_05595"
     CDS             4431..>5240
                     /locus_tag="PKY68_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24544.1"
                     /translation="MQQKTLYILLACIVLLFASGIFLFIDRHSKSRLINEITAQFELE
                     KEELEDGYSQLALQYESYGIRIANDSLAELLDIERTKNLRLLEEIRTLKINNVVRINE
                     LRKELETARSVMRSYIIQIDSLNILTTRLRKENETVSRQYREIKQEASQLARENTELT
                     EKVVLASILEARDINIRTLNERERVINRLSKIATIEFNFYLPKNISAKVGEKNIYLRI
                     LQPDNTPLVKNEGNLFEFEGTKLNYSIFRPIEYAGEETPVSLYWKVEEFLYP"
BASE COUNT         1457 a         1215 c         1098 g         1470 t
ORIGIN      
        1 tggcaaacca gtaaaaaaag agggatagca acaccaatac ggaaaaaaca acagaaatcg
       61 ccgaaagagt catccctata ccataggggt cattctttgc aaacttttct actttttcat
      121 tcctatccaa agtaaggttg ttggtgctgg gggtgacgcg acgcataatc tcccagtttt
      181 cttcgccgtc gatagtacgg gcatagctaa ggtcggcagg caatatcgga acgaccacac
      241 tgtcgattaa gcttttaccg tcagcgttaa aaaagtacaa aacgtttaca ctggcagtgt
      301 cgagggtgaa attgacataa aaattacctt tatgcggtgt accgtcggca taaaacaaga
      361 cgtgctgacg ttgttgaatt ttggtcagga cgtctccctt aggtatcata tacatagtgg
      421 gattgttccg gtcgttactt aaataacagc cgccaatgtc gactataccg tacgaggcgt
      481 tgaataattc aaaccacgcc gacctttgac catattcatc aatgaaattt gttttgttgt
      541 ttatcaaaac ttcattgaaa cagacccggg tcacctgggc ataaagcaca gtactcagaa
      601 acgttattaa aacaaaaaaa ccgattcgta tcttcataat tacaagggaa tgttaccgtg
      661 ttttttggca ggattggtca attttttggt ttgcagctgc tgcaaagctc tgatgacgcg
      721 gaaacgtgtg tttctgggtt cgattacatc atcaatgtaa ccatattgag cggctttgta
      781 gggattctga aaagcttcgc tgtattcagc ctctttttcg gcaaccagtt tagcgggatc
      841 ttcggcggcg gccacttcct tggcgtacaa aacttcaaca gcacctgaag ctcccataac
      901 ggcaatttcg gccgttggcc aagcataatt aatatcgccg cgcaattgtt tgcaactcat
      961 cacaatatga gctcctccgt aggatttgcg caaggttaca gtcaccttgg gcaccgttgc
     1021 ttctccataa gcgtacagca gctttgctcc gtgtataatc acaccaccgt attcctggcc
     1081 ggtaccgggc aaaaatccgg gcacgtcaac caaactcaca ataggaatgt tgaaggcatc
     1141 gcaaaagcga acaaaacggg ctccttttct ggaggcattg cagtcgagta caccggccag
     1201 cattttgggc tgattggcca ctatacccac cgattgtccg ttgaaacggg caaaaccgat
     1261 gattatattc ttggcataat ctttatgtac ttctaaaaac tccccattgt ctaccacggc
     1321 acctatcacc tcgtacatat cgtagggttt gttggggctg tcggggataa tatcgttcaa
     1381 ggaatcttcc aggcgatcga tgggatcggt gcaatccttc attggggctt cttcaagatt
     1441 attctgagga atataaccaa gtagtttcct tatcagagcg atgccctctt cttcgttatc
     1501 tacggcaaaa tgggttactc ccgacttggt tgcgtgtatt gaggcacctc cgagttcttc
     1561 ctgagtaaca tcttcgcccg tcactgtttt taccactttg ggaccggtca aaaacatata
     1621 ggacgttccc ttggtcatca gggtaaaatc ggtcaatgca ggagaatata cggctccgcc
     1681 tgcacaggga ccaaaaatgc cggaaatttg agggatgaca cccgaagcca aaatattgcg
     1741 ctcaaaaatt tcgccgtaac cggccagagc attgataccc tcctgaatac gggcaccgcc
     1801 cgagtcgttg atcccgataa ccggagcgcc catcttcatg gcctgatcca ttactttaca
     1861 aattttcagc gacatggttt ccgataagga acctccaaag acggtaaagt cctgggcaaa
     1921 aacatacacc agacgacctt cgatggttcc gtaaccggtt accactccat cgcccaaaaa
     1981 atgtttcttg tcctgatcaa aattggtaca acgatgtttt acaaacatat cgaactcttc
     2041 gaaactgcct tcgtccaaaa gcatgttgat acgctcacga gcagtatatt tccctttggc
     2101 atgctgtttt tcgatcgctt ttattccgcc gcccaaacga gcttgttcgc ggaaagcaat
     2161 taattctttt actttttcca gttgattact catcgatatt agtatttatt tgttaggatt
     2221 ttcgcacaat tcagtcaata cacccaatgt gaatttcgga tgtaaaaagg cgatgttcag
     2281 gccttcggct cctttacggg gagttttgtc gattagacgt acgccttttt cggctaccgt
     2341 atcgagcttt tcctgtaaat tttcgacggc gaaagcaata tgatgcacac cctctccgcg
     2401 attctctatg aatttaccta tgggcccttc gggatcggtc gactccagca actcgatttt
     2461 agtttgtccg accttgaaaa aggctgtttt tactttttgt tcgggcactt cttctatggc
     2521 atagcatttc aggccaaaaa tgttttcaac gtatggaatg gcagtctcta aactcttgac
     2581 tgcaatgcca agatgctcaa tatgtgttgg tttcataatt atgaattaag ttttaaaaaa
     2641 aacgctgcaa agataggaat taatatccgg cttaggtccg gggaaaaacc caaaaaatgt
     2701 attatttttt acataaatat tcctgtttca gcaagccctt tcaagaaact caaagcttct
     2761 gtcgcattca tttcttcggt caggtcgaaa atggagactt gcttaccctg atggttgatt
     2821 ttttcgttat tcaatatttt agccggaatt tgttcaaata ccgtaagtag ttgacgaccg
     2881 aaggggaaag tttcaaaagg caataaggcc ttgtcccggt caagatccga ttcaaaacgc
     2941 tgtaattcca gcaaggcctc tacgggtccc gccgacaggt gtacgccgtt ttgactttgg
     3001 gagatttcgc ccagacccaa ttcccgggcc cgaagacaca ggtcgtttct gagactcccg
     3061 ggcatggcct tttggggatt ggttgctccg gcaaaactgt ttctcaaatc cgaccagtct
     3121 gtatcgcccg aaatattcat taccacgatg cttctgccgg gttgggtaaa atgctccaat
     3181 tgccttggat ggaaagcatt caacacgtat accgtatctg aatctatacg ccatttttcg
     3241 gcataagtac cgccggccag tttatggttc tgaccgtttt gccacaggca atccagcgaa
     3301 cgcgaattaa aaaagggata ggtttccaac acttcaaggc ctcccagaat acgggcctca
     3361 ctgggttcca gtccaaaaac ttccctaaac cggcctttag cctcatcgcc cagtgttttc
     3421 gccccctcag aggccactcg gctgattact ccgtagtgac tacgtatgag atcgtgcttc
     3481 ttcaaatagg cagccggcaa aatacgaatg ctgtgtatac gcagttcgaa agcagccagc
     3541 ttatccaaaa tcagcgtcag tacctctttg agccggatag ccggatcggc tctggtaatt
     3601 tcgggcttga taaaaaacaa ggcattgttt tttaaaaagg tcgcctctgc atcggtgtaa
     3661 atggtttgaa tgttggaatg cgagtcctga gcatcggcta taccgttaag caatgcatca
     3721 atgattccgg agttgttgtt catgctgttt agattaaagt acgattttgt ttcgcagggt
     3781 tttttctttt tcttccgacc aatatccaca gtccaattca ataaatacgg aagtataggg
     3841 gatctgtgat tccgtcttta ttaccaccac attaaacaat cgggcagctt cgtcgaggcg
     3901 acggaaaata tcctcgtgcg gaatctgttt caaagtgtag ttggaacaaa tacgctgcaa
     3961 ggtggtttta aagtcactta cccccttaca caattcatcg tccataaatt ccaattcttt
     4021 gtcaagataa attatggggc gcagatgtga tgctgcttcc agtgcattga gcacttcctt
     4081 gagcacttta ttcaggtctt ctcccgaatt aagataactg atgcccttag acgattgcaa
     4141 aggataagct ttgtccacca ccaaaatcca gtttcgatgg cccagcaagg gcaataaact
     4201 gttcaactct gtcaaatatt tcatgccata caattatgat agggtattaa gaatttcctc
     4261 tatgtaaaaa aatcttttcg aggacgaaag tagtaattat atctcaatta gaagtcatct
     4321 tttagagcct taactcttaa agcattattt attatttttt tttatttgta cgaaagataa
     4381 cggggcaatt ttactatctt tgccccgtca ccctgcaaag attgatgttc atgcaacaaa
     4441 agactctata tattttactt gcctgcattg ttctgctttt tgcctcaggt atctttttgt
     4501 ttattgacag acacagcaaa agccggttga tcaacgaaat taccgcacag tttgaactgg
     4561 aaaaagaaga actcgaagac ggatattccc aactggccct gcaatacgag agttacggca
     4621 tccgtattgc caacgattcg ctggccgaac tgctcgatat tgaacgaacc aaaaatctgc
     4681 gtctgctcga agaaatcagg actcttaaaa tcaacaacgt ggtccgtatc aacgaacttc
     4741 gcaaagaatt ggaaacagcc cgcagtgtta tgcgatccta cattattcaa atcgactcgc
     4801 tcaacatcct gactacacgt ttgcgtaagg agaacgaaac cgtatccaga caataccgtg
     4861 aaatcaaaca ggaagcctcc caattggcca gggaaaatac ggaactgacc gaaaaagtag
     4921 ttctggcatc catactcgaa gcccgagata taaatatcag aaccttaaac gaaagggagc
     4981 gcgtaataaa ccgtttgagc aaaatagcta ccatagaatt caatttttac ctgcctaaaa
     5041 acatttcggc caaagtgggc gaaaaaaata tttacctgcg catactccaa ccggacaaca
     5101 ctcctttggt aaaaaatgag gggaatttat ttgagttcga gggtactaag ctcaattatt
     5161 ccatttttcg tccgatagaa tacgccggag aagaaactcc ggtcagcctg tactggaaag
     5221 tcgaggaatt tctgtatccg
//
LOCUS       DAOSGL010000148         5225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14077_length_5225_cov_4.049516, whole genome shotgun sequence.
ACCESSION   DAOSGL010000148 DAOSGL010000000
VERSION     DAOSGL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14077_length_5225_cov_4.049516"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2186)
                     /locus_tag="PKY68_05600"
     CDS             complement(<1..2186)
                     /locus_tag="PKY68_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOH24545.1"
                     /translation="MAKSSGSKSKKKASKNSVLSRILLVLWITFVTVVLLLALLFTKI
                     AQGKLGYMPPIEELENPKNKYATEIYSSDGILLSTYYVDEGNRVEVQYRDLSPHLVRA
                     LIATEDLRYTRHSGIDIKALFRVFFKRVILRQQTAGGGSTITQQLAKQLYTPPAQSRF
                     ERAMQKPIEWVIAVQLERFYTKEEILTMYLNKFDFRYNAVGIKTASWVYFATTPDKLN
                     MEQAATLVGMFKNPVLYDPLRYNQRTKERRNLVLEQMQRAGYIDRAVCDSVQDLELET
                     DYRKVDHSLGLAPYFRQYLRLMLTHTEPKRKNYASWQDQLFKDDSIAWETNPLFGWCH
                     KNVKPDGSNYNLYTDGLKIYTTIDSRMQQYAEEAVYEHIAMDLQPKFFEEKAGRYYAP
                     YTNNLRAGQVDTILWTSARQTERYRVLRKAGLSEQEIRKSFRTKVEMQVFSYQGMIDT
                     VMSPMDSIRYHKHFLRAGFMSMDPYNGHVKAYVGGVSFKPFEYDMVMLGRRQVGSTIK
                     PFLYTMAMEEGFSPCDPVRNEPITLMTETGEHWTPKNTSTKMVGEMVNLRWGLANSNN
                     WISAYLMSKLSPYSFVRLLRSFGIKGHLDPVVSLALGSCEVSLSEMVSAYTAFVNGGI
                     KVMPLFVTRIEDNDGNVISYFTPQMSEVFSEHTSYQMQVMLRAVINEGTGIRVRSRYG
                     ILADMGGKTGTTQNNSDGWFLGFTPKLVSGAWGGGEERDIHFDYMS"
     gene            2292..3203
                     /gene="pyrB"
                     /locus_tag="PKY68_05605"
     CDS             2292..3203
                     /gene="pyrB"
                     /locus_tag="PKY68_05605"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOH24546.1"
                     /translation="MSYPSLVSIADYSKEQMLHILELAAGFESNPNQNILQGKVAATL
                     FFEPSTRTRLSFETAINRLGGRVIGFSDAATSSSTKGETLRDTIKMVSNYADIIIMRH
                     FLEGAARYATEVTDIPIINAGDGANQHPTQTMLDLYSIQKTQGRLDNLKVVMVGDLKY
                     GRTVHSLLMALKYFSPVFYFIAPEELLMPNEYKLICRENNIPYYENSNFEPEIIEQAD
                     ILYMTRIQRERFTDLVEYEKVKNVYILHNHMLDKSKNNLRILHPLPRVNEISQDVDDN
                     PKAYYFEQARNGLYVRQAIISHALGLV"
     gene            3215..3682
                     /gene="pyrI"
                     /locus_tag="PKY68_05610"
     CDS             3215..3682
                     /gene="pyrI"
                     /locus_tag="PKY68_05610"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOH24547.1"
                     /translation="MDKKYVKKEMQVKALENGTVIDHIPANKLFKVVEILQLEQISNE
                     VTIGNNLASKRMGTKGIIKVANRFFESKEINRIALIAPNAKINIIRDYQVTEKRIITL
                     PDHFKGIVKCVNPKCITNNEVMEPRYNVISKSPLVLKCPYCEREIREEELQLL"
     gene            3730..4296
                     /locus_tag="PKY68_05615"
     CDS             3730..4296
                     /locus_tag="PKY68_05615"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOH24548.1"
                     /translation="MKQHRKPGTMIYPLPALLVSCGSTPDEYNLITVSWTGTVCSEPP
                     MCYISVRKTRHSHKILERNKEFVLNLTTKDLSYATDWCGVRSGEKFNKFKEMRLTPGK
                     AAVVQAPTVEESPVNIECRVKEILSLGSHDMFLAEVVNVQVDEKYIDQATGKPDLEKA
                     GLQVYVQGSYFELGKLIGKSGWSVEKKK"
     gene            4322..>5225
                     /gene="glyA"
                     /locus_tag="PKY68_05620"
     CDS             4322..>5225
                     /gene="glyA"
                     /locus_tag="PKY68_05620"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOH24549.1"
                     /translation="MKRDQIVFDIIEKERKRQLRGIELIASENFVSEQVMQAMGSCLT
                     NKYAEGYPGNRYYGGCEVVDESESLAIERLKKLFNAEYANVQPHSGAQANAAVFMAVM
                     NPGDTFLGLNLSHGGHLSHGSPVNSSGIYYKALEYNVKEETGRVDYDQMEEIALREHP
                     KLIVGGASAYSRDWDYARMRSIADKIGALLMIDMAHPAGLIAAGLLNNPLEHAHIVTS
                     TTHKTLRGPRGGIILIGKDFDNPWRKVTPKGEIRKMSSLIDSAVFPGIQGGPLEHIIA
                     AKAVSFGEALQPEYKQYQRQVKKNA"
BASE COUNT         1505 a         1279 c         1144 g         1297 t
ORIGIN      
        1 tcggacatat aatcaaaatg aatatcccgt tcttcgcctc cgccccaggc cccactcacc
       61 agtttaggcg taaagcccaa aaaccagccg tccgaattgt tttgagtagt cccggttttg
      121 cctcccatat cggcgagaat tccatacctg gaacgaaccc ggataccggt accttcgttg
      181 ataacggcac gcaacataac ctgcatctga taggaagtgt gctcactgaa cacttccgac
      241 atttgaggcg taaagtaact gattacatta ccgtcgttgt cttctatcct ggtgacaaac
      301 aagggcatta ccttgatgcc tccgttgaca aatgccgtgt aggcactcac catctcggaa
      361 agggagactt cgcaggagcc taatgccaga gaaaccaccg gatccagatg acctttgatt
      421 ccaaaagagc gcagtaaacg cacaaaactg tagggagaca atttactcat caggtaagcc
      481 gaaatccaat tgtttgaatt ggccaagccc caacgcagat ttaccatctc gcccaccatc
      541 ttggtggatg tgtttttggg ggtccaatgc tctccggttt cagtcattag ggtaatgggt
      601 tcgttgcgta ccggatcgca gggcgaaaaa ccctcttcca tggccatggt ataaagaaat
      661 ggttttatgg tcgaacccac ttgcctgcgg cccaacatca ccatgtcgta ttcaaaaggt
      721 ttaaagctga caccgcccac ataggccttg acgtggccgt tgtagggatc cattgacata
      781 aatccggccc gcaaaaaatg cttgtggtag cggatggaat ccatgggaga cataacggtg
      841 tctatcatgc cctgatacga aaacacctgc atttccacct tggtgcgaaa ggatttgcgt
      901 atttcctgct ccgacaaacc ggcttttcgc aacacccggt aacgttctgt ctgccgggca
      961 ctggtccaca aaatagtatc cacctgccct gctctgaggt tgttggtata aggagcataa
     1021 tatcttccgg ctttttcttc gaagaatttc ggttgcaagt ccatagcaat atgttcgtag
     1081 acagcttctt cggcatattg ctgcattctg gagtcgatgg tagtgtaaat cttgaggccg
     1141 tctgtatata aattatagtt ggagccgtcc ggttttacat ttttatgaca ccagccgaac
     1201 aaagggttgg tctcccaggc aatggagtcg tccttaaata actgatcctg ccaggaggcg
     1261 taattttttc gttttggctc ggtatgcgtc aacatcagcc tgagatattg acggaaataa
     1321 ggggcgagcc ccaggctatg gtcaacttta cgataatcgg tttccaattc cagatcctgt
     1381 acggaatcgc atacggccct gtcgatgtag ccggcccttt gcatttgttc cagcaccaga
     1441 ttacgcctct ctttggtgcg ttgattataa cgcaggggat cgtaaagcac cggatttttg
     1501 aacataccta ccagggtggc cgcttgttcc atattcaatt tgtcgggagt agtggcaaaa
     1561 taaacccagg aagctgtttt gattcctacg gcattgtacc ggaaatcgaa cttgttcagg
     1621 tacatggtca gaatctcttc tttggtataa aaacgctcca gttgtacggc tatcacccac
     1681 tctatgggtt tttgcatggc acgttcaaat cggctttgag ccggaggagt atacaattgc
     1741 ttggccagtt gttgggtaat ggtgctcccc ccaccggctg tttgctgccg caagatgacc
     1801 cgtttaaaga acactctgaa caaggctttt atgtcgatgc cggaatgcct ggtatagcgc
     1861 aggtcttcgg tagcgatcaa ggctctgacc agatgaggcg agagatctct gtattgtacc
     1921 tccacccggt tgccttcgtc cacataatag gtagacaaca aaattccgtc cgaagaatag
     1981 atttcggttg cgtatttatt tttaggattt tccaattcct caatgggagg catataaccc
     2041 agtttgccct gagctatttt ggtaaaaagc aaagccagca aaagcactac cgttacgaag
     2101 gtgatccata acaccaacag aatcctggaa agaactgagt tttttgaggc tttttttttc
     2161 gatttcgaac cagaagattt agccatttgt atagagtttt gttcagggaa caaaaggact
     2221 cgcaaaggta aaaaaaattt tcgcagatta aaaagcttta tatacctttg tcaggcctaa
     2281 aatacgattt tatgtcttat ccaagtctgg tttccattgc ggattattcc aaggaacaaa
     2341 tgctgcacat tctcgaattg gcagccggct ttgaatcaaa tcccaatcaa aacattttgc
     2401 agggcaaagt agccgcgact ctgttttttg aaccatccac ccgcacccgt ctcagtttcg
     2461 aaactgccat caaccggctc ggaggacgcg taatcggttt ttccgatgcg gccacatcca
     2521 gttccaccaa aggagaaacc ctcagagaca ccattaaaat ggtttccaac tatgccgaca
     2581 taatcattat gcggcatttt ttggaaggag ctgcccgcta cgccaccgaa gtaaccgata
     2641 tccccatcat caatgccgga gacggagcca atcaacaccc cacccaaacc atgcttgatt
     2701 tatactccat tcaaaaaact cagggccgac tggataatct caaggtagta atggtaggcg
     2761 atctgaaata cggaagaacc gtgcattcac tgctaatggc cctgaagtat ttttctcccg
     2821 ttttctactt tatcgctccc gaagaactgc ttatgcccaa tgaatacaag ctgatttgcc
     2881 gggaaaacaa catcccctat tacgagaatt ccaattttga accggaaatc atcgagcagg
     2941 ccgacatcct gtacatgacc cgcattcaac gggagcgttt taccgacctg gtggaatacg
     3001 aaaaagtaaa gaacgtttat attctgcata atcacatgct ggataaatcc aaaaacaacc
     3061 tccggatttt gcatccccta ccccgggtga acgaaatttc gcaggacgtg gacgacaacc
     3121 ccaaggctta ttatttcgag caggcccgca acggattgta tgtaagacaa gccattatca
     3181 gtcatgcttt aggtttagtt taaatcccac aacgatggat aaaaaatacg tcaaaaaaga
     3241 aatgcaggtc aaagcactcg aaaacggaac cgtcatagac catatccccg ccaataagct
     3301 gtttaaggtg gtggaaattc tgcaactcga acaaatttcc aacgaagtaa ccattggcaa
     3361 caatttagcc agcaaacgca tggggacaaa aggcatcatt aaagtggcca accgcttttt
     3421 tgaatcaaaa gaaatcaacc gcatcgctct gattgctccc aatgccaaaa taaacatcat
     3481 acgagattac caggtaacag aaaaaagaat aatcaccctt cccgatcatt tcaaaggtat
     3541 agtcaaatgc gtcaatccaa agtgcatcac caacaatgaa gtcatggaac cccgttataa
     3601 cgtaatcagt aaatctcctt tggtactgaa atgcccctat tgcgaacggg agatcagaga
     3661 agaagaatta cagttattgt aaaagtaaac tgagtcaagc ttccagcact ccaaaaaaat
     3721 aaaaaaaata tgaaacaaca tcggaaaccc ggaacaatga tttatccact tccggctctt
     3781 ttagtcagct gcggttccac cccggatgag tacaacctaa ttaccgtaag ttggacgggc
     3841 actgtttgca gcgaaccacc catgtgctac atttctgtcc ggaaaaccag gcattctcac
     3901 aaaatcctcg aaagaaacaa ggaatttgtt ttaaacctga ccaccaaaga cctctcctat
     3961 gccaccgact ggtgcggagt acgttccggt gaaaaattca ataaattcaa agaaatgcgc
     4021 ctgactccgg gcaaagccgc agtggtgcag gctcccacag tcgaagaatc tcccgtcaac
     4081 attgaatgca gggttaaaga aattctctct ctgggctctc acgacatgtt tctggccgaa
     4141 gtggtcaatg tgcaggtgga cgaaaaatac atagatcagg ccaccggaaa accggacctg
     4201 gaaaaagccg gcttgcaggt gtacgtacag ggctcctact ttgaattggg taaattaatc
     4261 ggtaaatccg gctggtcggt cgaaaagaaa aaataattca ttcattccta tactttattt
     4321 tatgaaaaga gatcaaatcg tttttgatat catcgaaaaa gaacgtaaac gccaactcag
     4381 aggcatagag ttgattgctt ctgaaaattt tgtaagcgaa caagtcatgc aggccatggg
     4441 ttcctgccta accaacaaat acgccgaagg ttatccaggg aatcgctact acggaggatg
     4501 cgaggtggtt gacgaaagtg aaagcttagc tatagagagg ctcaaaaaac tttttaatgc
     4561 agaatatgcc aacgttcaac cacattccgg agcccaggcc aatgctgctg tttttatggc
     4621 ggtgatgaat cccggtgata catttctcgg tttgaatctc tcacacggcg gccacctttc
     4681 gcacggctct ccggtaaaca gttcaggtat ctattacaaa gcactggaat acaatgtaaa
     4741 agaagaaacc ggacgggtag actacgatca aatggaagaa atcgccctgc gcgaacaccc
     4801 aaaactcatt gtgggcggag cctctgccta ttcccgcgac tgggattatg cccgcatgcg
     4861 ctccattgca gacaaaatcg gcgctttgct tatgatcgac atggcgcatc ccgccggact
     4921 gattgccgcc gggctgctta acaatcccct cgaacacgct catatcgtta cttcgactac
     4981 tcacaaaacg ttgcgaggcc cccgcggcgg tattattctt atcgggaaag atttcgacaa
     5041 tccctggaga aaagttacac ccaaagggga aatccgcaaa atgtcgtcct tgatcgattc
     5101 agccgtcttc ccaggcattc agggcggacc gctggaacac atcatagcgg ccaaagccgt
     5161 ttcttttggt gaagccctgc aacccgaata caaacaatac caaaggcagg ttaagaaaaa
     5221 cgccg
//
LOCUS       DAOSGL010000149         5210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14146_length_5210_cov_3.384675, whole genome shotgun sequence.
ACCESSION   DAOSGL010000149 DAOSGL010000000
VERSION     DAOSGL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14146_length_5210_cov_3.384675"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1404)
                     /locus_tag="PKY68_05625"
     CDS             complement(52..1404)
                     /locus_tag="PKY68_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOH24550.1"
                     /translation="MTFADVHLYPGKEQAEFIALNSTDSNSLKKLFESAETEYVLLAK
                     QSQGLNFKPDGLFRLQQVTCDSRAGMVYTNYLEMKDGQYQAHPLNPCQLGSVRDDFDF
                     GACCLIRREAAVKALADCSENYRYAGFYDLRLRLSEHFPIVHLEEYLYCLQTGDVEDS
                     EKAHFAYVDPKNRDYQLEMEAVCTAHLKRIGAWLAPGAEPVELNEGNFPTEATVIIPV
                     RNRARTIADAVQSALKQQCSFDFNLIVVDNHSTDGTGEVLKSLAAADKRLTVIEPESK
                     LLNIGRCWNLAVKHPLCGRFAVQLDSDDIYSGPHTLERVVEAFYREQAAMVVGSYRIC
                     DFELNTLPPGLIDHREWTEENGRNNALRINGLGAPRAFFTPILRSNLLPDTSYGEDYA
                     VCLRISRRWKVARIYEELYLCRRWEGNTDAQLDLKSLNANNAYKDKLRSLEIMARQAL
                     "
     assembly_gap    1513..1612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1613..2239)
                     /locus_tag="PKY68_05630"
     CDS             complement(<1613..2239)
                     /locus_tag="PKY68_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH24551.1"
                     /translation="MSKLKIIGFDADDTLWENGLYFRQAEQEFAVLLKHYIDPDSLHA
                     KLYAVEMKNMPTYGYGAMAYTLSLIETALDVTQSDLDPEIIERILGLGRDILTHPVEL
                     LPDVENVLPVLQSRYRLIIVTKGDILDQERKLGKSGLLPYFHHIEIVSEKHEDNYRDL
                     LRRLDIEPAEFMMVGNSLKSDVLPVLNIGGQAVHVPAASIWEHEQCEKP"
     gene            complement(2239..3018)
                     /locus_tag="PKY68_05635"
     CDS             complement(2239..3018)
                     /locus_tag="PKY68_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOH24552.1"
                     /translation="MRISLLQTAIAWAKPEVNLNKYLGLLSALKGKSDLAVLPEMCLS
                     GFGAPAPKISETNRGKCIGKLREATADYGLAVTGSFSALDEETKRYFNRGFFFTPDGR
                     SFYYDKKHLFAMGDENKSYTAGNKQLNINYLGWNIRLIICYDLRFPLWCRNRHNEYDL
                     LLVSANWPQARQKAWTTLLQARAIENLAYVCGVNCVGEDGRGIVYGGDSLLLDYKASL
                     LSAMKNGREQAETHTLDLDALRDFRQKFPAWKDADDFHFSE"
     gene            complement(3022..3573)
                     /locus_tag="PKY68_05640"
     CDS             complement(3022..3573)
                     /locus_tag="PKY68_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOH24553.1"
                     /translation="MSFKKRFSRFLFKCAGWKAVALTPDYNKCVLVVAPHTSNWDFVV
                     GKLAYNALGREGNFLIKKEWFRFPFNYFFKAIGGIPVDRGKRQRMTEAIIDLFSERDH
                     FCLGITPEGTRKPNARWKRGFYHIAINAKVPIVLVKLDYGNKCVTLFRVFHPSGDEEA
                     DIRAVKEAYRGVRAKHPKQFLID"
     gene            complement(3601..4113)
                     /gene="nadD"
                     /locus_tag="PKY68_05645"
     CDS             complement(3601..4113)
                     /gene="nadD"
                     /locus_tag="PKY68_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOH24554.1"
                     /translation="MKRIGIFGGTFDPIHYGHISVAEHALRQAGLDELWFVVSPHNPL
                     KKEEEIKEAGQRLQEARKALTEFPEFKVSDIEFNLPRPSYMAQTLRTLQKKYPGNDFV
                     LIIGGDNLEVFEQWKDYRFLLDNFDILVYPRPGSPNRVPGEWTRVSLLDAPLMDVSST
                     RIRKTQQGRK"
     gene            complement(4110..4979)
                     /locus_tag="PKY68_05650"
     CDS             complement(4110..4979)
                     /locus_tag="PKY68_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOH24555.1"
                     /translation="MTLSMTGYGKAQADIGKRKISIELRSLNSKQFDLSMRIPSLLKE
                     KETEIRNEIQKRLERGKIDLLIQVETEQTDTPCSINRSLLETYYRDILETAGALGINP
                     PSDPWPVLLKLPDVLKGEHPDMEAEEWTEIMELLNRAIDQLNGFRKQEGEMLKAFFQN
                     KLQAIESLLGELETYESERVDKIKSRLEEGLKKVAVQDYDRNRFEQELIYYIEKLDVS
                     EEKSRLINHCEYFKTTLNAGSKGKKLGFIVQEMGREINTLGSKANHAEMQRLVVCMKD
                     ELEQMKEQVLNVL"
     gene            complement(4976..>5210)
                     /locus_tag="PKY68_05655"
     CDS             complement(4976..>5210)
                     /locus_tag="PKY68_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA synthetase"
                     /protein_id="HOH24556.1"
                     /translation="GQVVKASIILAKEYRDHDKEALAKEIQEHVKQVTAPYKYPRVIE
                     FVNELPKTISGKIRRVEIRQDDSEKINVKKRQP"
BASE COUNT         1145 a         1213 c         1306 g         1446 t
ORIGIN      
        1 tatcggccgc caagggccag agcttgagtt gttgttcgaa gaatttttct gtcataaggc
       61 ttgtctggcc atgatttcca gacttcgtaa tttgtcttta taggcattgt tggcgtttag
      121 cgacttcaaa tctagttgag cgtcggtatt tccttcccag cgacggcaga ggtataattc
      181 ctcgtaaatg cgggctactt tccatcgcct tgagattctg aggcagacgg cgtaatcttc
      241 tccgtagctg gtgtcgggca ggaggttgct gcgtaatatg ggcgtgaaaa aggcccttgg
      301 agctcccagg ccgttgatac gcaaggcgtt gtttctgccg ttttcttccg tccattccct
      361 gtgatcaatg agaccgggcg gcaaagtgtt caattcaaag tcacatatgc ggtaactgcc
      421 taccaccatg gcggcttgtt cccggtaaaa ggcctctact acgcgctcca gagtgtgagg
      481 accgctgtag atgtcgtcgc tgtcgagttg tacggcaaaa cgtccgcaca ggggatgttt
      541 gacggccaga ttccagcagc ggccaatgtt cagcagtttg ctttcgggct caatcactgt
      601 caggcgttta tcggctgccg ccaaactttt caaaacctcg ccggtgccgt ctgtggaatg
      661 attgtctacc actatcaggt tgaaatcgaa agaacattgc tgtttcaagg ccgattgaac
      721 ggcatcggcg atggtacgtg cccggttgcg gacaggaatg atcaccgtgg cttcggtggg
      781 gaaattaccc tcgttcagtt cgacaggttc ggctcccgga gccagccagg ctccgatgcg
      841 cttgaggtgt gccgtacata cagcctccat ttcgagctgg tagtccctgt ttttgggatc
      901 cacgtaggca aagtgagcct tttcgctgtc ttccacatcg ccggtttgga ggcaataaag
      961 gtattcctcg aggtgaacaa tcggaaaatg ctccgaaagc cttaacctca gatcgtaaaa
     1021 gccggcataa cgataatttt cggaacaatc ggcgagtgcc ttgacggctg cctcgcggcg
     1081 tatcagacaa caggcgccaa aatcaaaatc gtcgcgtaca ctgcccaact gacaggggtt
     1141 tagcggatgg gcttggtatt gtccgtcctt catttccagg tagttggtat aaaccatgcc
     1201 ggcgcggctg tcgcaggtca cttgctgtaa acgaaacaag ccgtccggct taaagttcaa
     1261 tccctgactt tgtttagcca agaggacgta ttcggtttcg gccgattcaa acagtttttt
     1321 gaggctgttg ctgtcggtgg aattcagagc aatgaactct gcttgttctt ttccgggata
     1381 cagatggacg tcggcaaaag tcatgttaaa acggttaata atttaccgag gcggtcgatt
     1441 tcatagtagg gtatggaggg cttttcgcat tgttcgtgtt cccagatgga ggcagcggga
     1501 acgtgaacag ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggctttt
     1621 cgcattgttc gtgttcccag atggaggcag cgggaacgtg aacagcctgt cccccgatgt
     1681 tgagcaccgg cagcacatcc gacttaaggg aattgcccac catcataaat tcggcaggtt
     1741 caatatcgag gcggcgcagc aaatccctgt aattgtcttc gtgtttttcg gacacgattt
     1801 ctatgtggtg aaaatagggc aggagccccg atttaccgag tttgcgttcc tgatccagaa
     1861 tatcgccctt ggttacgatg atcagacggt agcgcgattg aagaacgggc aatacatttt
     1921 ccacatcggg caagagctcc accggatgag tgaggatgtc gcgtcccagg ccgagaatac
     1981 gttctatgat ttcgggatcc aagtcagact gagtgacatc cagggctgtt tctataagcg
     2041 acaaggtata ggccatggct ccgtagccgt aagtgggcat atttttcatt tcgacggcgt
     2101 agagtttggc gtgcagactg tccggatcta tgtaatgctt cagcaaaacg gcaaattctt
     2161 gctcggcctg cctgaaatag agtccgtttt cccataaggt gtcgtcggca tcaaatccta
     2221 taatcttcag tttgctcatt attcggagaa atgaaagtcg tctgcgtctt tccaggcagg
     2281 aaatttttgc ctgaaatccc tgagggcatc taaatcgagt gtgtgtgttt cagcctgctc
     2341 ccggccgttt ttcatggccg aaagcaaact tgctttgtaa tcgagcaaca gggaatcgcc
     2401 cccgtaaact atgccccggc cgtcttcacc cacacagttg acaccacaga cgtaggcgag
     2461 gttttcgatc gctctggcct gtagcagggt agtccaggct ttttgacggg cctgcggcca
     2521 gttggctgac accagcaaca gatcgtattc gttgtgccgg ttgcgacacc aaagcgggaa
     2581 acgcagatcg taacagataa tgagccggat attccaaccc aggtaattga tattaagttg
     2641 tttgttgccg gctgtatagc ttttgttttc gtcgcccatg gcgaacaaat gtttcttgtc
     2701 gtaataaaaa cttcggccgt cgggagtaaa aaagaatcct ctgttgaaat aacgcttcgt
     2761 ctcttcgtct aaggccgaaa aactgcccgt tacagccagt ccgtaatcgg cagtggcttc
     2821 acgcagcttg ccgatgcatt ttccccggtt ggtttccgaa attttcggag ccggagctcc
     2881 aaaaccactc aggcacattt cgggcaggac ggccaaatcg cttttgcctt ttaaagcgga
     2941 gagcaaaccg agatacttat tcagattgac ctcgggttta gcccaggcaa tggcggtttg
     3001 taacagactg attcgcatgc ttcaatcgat taaaaattgt ttggggtgtt tggccctgac
     3061 accgcgataa gcttctttga cggcccggat gtcggcttct tcgtcaccgc tgggatgaaa
     3121 tacccggaac agagtgacgc atttgttgcc gtaatcgagc tttaccaaga cgatgggcac
     3181 cttggcgttt atggcgatgt ggtaaaagcc cctcttccat ctggcgttgg gtttgcgggt
     3241 gccttccggg gtaatgccca gacaaaaatg atcgcgttcg gaaaacaagt ctatgatagc
     3301 ctcggtcata cgttgtcttt ttcccctgtc tacagggata cctccgatgg ctttaaaaaa
     3361 atagttgaag ggaaagcgaa accattcttt ttttatcagg aaatttcctt ctctgcccaa
     3421 ggcattgtag gccagttttc cgacaacgaa atcccaattg ctggtgtggg gggcaactac
     3481 caacacacat ttattgtagt ccggtgtaag ggcaacggcc ttccatccgg cacatttgaa
     3541 caagaagcgt gagaaacgtt ttttaaatga catagagtct gagtgtttaa gaactggtgt
     3601 ttattttctt ccttgttgtg ttttgcgaat ccgggtggag gacacatcca tcaggggagc
     3661 gtcgagtaga ctcactctgg tccattcacc gggcacccgg ttgggactgc ccggacgcgg
     3721 ataaacgaga atgtcgaaat tatcgagcaa aaaccggtag tctttccatt gttcaaatac
     3781 ctccaggttg tcgcctccga tgatcaagac aaagtcgttc ccggggtatt ttttttgtag
     3841 cgttcgcagg gtttgcgcca tataagaagg acggggcaga ttaaattcta tgtcggaaac
     3901 tttgaattcc ggaaattcgg tcaatgcctt gcgagcttct tgcaagcgtt gtccggcctc
     3961 tttgatttcc tcttcctttt tgagtggatt gtgcggactg actacaaacc ataattcatc
     4021 gagtccggct tgtctcaggg cgtgttcagc cacagaaata tgtccgtaat gaatagggtc
     4081 gaaggtaccg ccaaaaatgc cgattctttt cataatacgt tcaatacctg ctctttcatt
     4141 tgttctagtt cgtctttcat gcacacgacc aggcgttgca tttcggcgtg attggcttta
     4201 gagcccaagg tgttgatttc acgccccatt tcctgtacga taaaacccag tttcttgcct
     4261 ttactcccgg cattgagagt ggttttgaaa tattcgcagt ggttgatcag gcgggatttt
     4321 tcttcgctta cgtccagttt ttcgatgtaa taaattaact cttgctcaaa acggttacga
     4381 tcgtaatcct ggacggctac tttctttagg ccttcttcca ggcgggattt tattttatca
     4441 acgcgttcgg attcgtaggt ctccagttcc ccgagcagag actctatggc ttgcagttta
     4501 ttttggaaaa aggctttcag catttcacct tcctgttttc tgaagccatt caattgatcg
     4561 atggcccggt tgagcagttc catgatctca gtccattcct cagcttccat atcgggatgt
     4621 tcgcctttca aaacatcggg caatttgagc agaaccggcc aggggtcgga gggaggattg
     4681 atacccagag caccggccgt ttcgagaata tcgcgataat aagtttccaa aagactgcgg
     4741 ttaatgctgc agggagtatc ggtttgctcc gtttccactt gtatcagcag atcgattttg
     4801 ccccgttcca ggcgtttttg aatttcatta cggatttcgg tttccttttc tttcaacaga
     4861 gaagggatgc gcatagaaag gtcgaattgt ttgctgttga gcgaacgcaa ctcgatactg
     4921 atttttctct taccgatatc agcctgagcc ttaccgtaac ctgtcatgga taatgtcatg
     4981 gctgtcgttt tttgacgttt attttttcgc tgtcgtcctg gcggatttcg actcgtctga
     5041 tcttgccgct gatggttttg ggcaattcgt ttacaaattc aattacacgc ggatatttgt
     5101 agggagcggt cacctgcttc acatgttcct gtatctcttt agccagggct tctttgtcgt
     5161 gatcgcggta ttctttggcc agaatgatgc tggctttgac cacttgccct
//
LOCUS       DAOSGL010000150         5189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14216_length_5189_cov_4.064472, whole genome shotgun sequence.
ACCESSION   DAOSGL010000150 DAOSGL010000000
VERSION     DAOSGL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14216_length_5189_cov_4.064472"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..2861
                     /locus_tag="PKY68_05660"
     CDS             33..2861
                     /locus_tag="PKY68_05660"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOH24557.1"
                     /translation="MTKLNKYLLTVVLFLPFLLMAQEQLTNLPTLYITTDSTYANGTK
                     VPIQKEVYFSGSLRVVAGSETPGQYDSTIQIRGRGNSTWNLAKKPYRIKLDKKTRLLG
                     MTAKENDWVLLANHADKTLVRNALAFEISKACNIYYTPDYRFVDVILNGNYIGNYMLT
                     DQVERDKDRVNTEKMDTSRLDPNLTGGYVLEIDGFGITGSYDPSKGELFPEAFSSNIG
                     NKVTIKYPDNDEITAAQRQYIIQHYNAFEQSILNYNNTQVAYENICRYLDTTAFVNWY
                     IANELTANPDGVWSIFMKKHRDDDRLYFGPLWDNDISFGNCNRIYSLANDGRSESIML
                     KCFSNNRVKELINKVLTISPIRKMIAERWKVIYQNEMLKDDLIHLLYNMEEELDASQK
                     LNYQKWSTALSSRIYNEIYYGSNISFAMQMEYLRNYLSERIDFLDSFFSAMANEGGID
                     PEDPPLPPVDTATYFSPEPYVWYHLQCQLNDKYLVCQELAGGYQLDYTYDPETAGDWA
                     KFEISCSKDDENRSVYYLRNKAAKTYVYINAAKAAIPLDPVQKTFFTLYPSPNEAGHY
                     GLMVLSDNSTLHELGIDANGSVVSWPTLNKKGQREYRFIPDRSHFQSVFDPPNPQQAL
                     WDIEENWDNNIPNAYATAIIAEGAKLELSQDETVTVDSLIILPGAEVLNYGTIRADVS
                     LFCSNDSLSASYDYSQETQLISERVLYRSFNRSQSWYALSLPLDVKSVRNKEMTILQP
                     GKDYYLMEYKADSNRFDSLSLAGSFKPKAGPYLFSVSPEREQQAFVFVFDTKSSYQTG
                     NSDYSFTGNTGFKTINIPAAGTYLLEDHLHFVLQEGQQLLLPAFHAYFSVSGDRLPDS
                     LAIYNMNPANDTLTLENISKQGPLLVSRAGLLIIFNMEAGEDFSLFKLDGRLLLRDKA
                     LNNPTYVPLKQGIYLLRYKKRFHKIHIP"
     gene            2883..>5189
                     /locus_tag="PKY68_05665"
     CDS             2883..>5189
                     /locus_tag="PKY68_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH24558.1"
                     /translation="MKKRIISTFMLTTIVLSVSAQKNEWQDPGINAVNRAEMHTNYFA
                     YESREAALKNCRESSSNYLSLNGMWKFYWVKDANNRPTDFFRSDFDDRNWANMPVPGI
                     WEMNGYGDPVYVNTGWAWRNQFRSNPPDIPVENNHVGSYRREITIPDHWKGKEIFAHF
                     GSVTSNIYLWVNGKYVGYSEDSKLEAEFNLSKYLRPGKNLIAFQVFRWCDGTYLEDQD
                     FFRLSGVGRDCYLYARPSNYIQDIRITPDLDAQYKDGSLKIDVSMKGKGELELNLLDE
                     NNETLASTQRNASGKFSLTMNLSNPQKWSAECPNLYTLVATLKGGGRVLEAIPVKVGF
                     RKIELKGDQLLVNGQAVLFKGVNRHEMDPDYGYYVSPERMIQDLRLMKEHNVNAVRTC
                     HYPDNNLWYDLCDEYGLYVVAEANIESHGLGYGERTTAKRPDYALAHMERNQRNVQRS
                     FNHPSVIFWSLGNEAGFGPNFEMCYKWIKAEDPSRPVQYEQARTNEFTDIFCPMYYGY
                     NNCIRYCEGDIQKPLIQCEYVHAMGNSEGGIKEYWDLVRKYPKYQGGFIWDFVDQSLR
                     KFDKNGVMFYAYGGDFNLYDASDGNFCDNGLISPDRLPNPHFHEVGHVYQSIWASASD
                     LEKGEIEIYNEYFFRDLSAFYLEWALLVNGKVCQSGMVDKIDIEAQQTKKLKLDYDLS
                     GISPDKEVLLNISFKLRKAEQLLPAGFVLAKNQLCIRDRSEQSLNFDNILTANIELQT
                     PKVIENDWRFLIIEADDYRIEFNKHNGYL"
BASE COUNT         1533 a         1254 c         1121 g         1281 t
ORIGIN      
        1 tacggaacct aagtccgtat atacctgtga taatgacaaa gctgaacaaa tacttgctga
       61 ctgtcgttct tttcctgccc tttctactaa tggctcagga gcagttgacc aatctgccta
      121 cattatacat taccacagat tcgacttacg cgaacggaac taaagtaccc atacaaaaag
      181 aggtttattt ttcaggctct cttcgtgttg ttgccggtag cgaaactccc ggacaatacg
      241 acagcaccat acagatcaga ggacgaggca attccacctg gaatctggct aaaaagccct
      301 atcgcatcaa gttggacaag aaaacaaggt tgttgggtat gactgccaaa gaaaacgact
      361 gggtgctttt ggctaaccac gccgataaaa ccctggtgcg caatgctctt gccttcgaaa
      421 tcagtaaagc ctgcaatatt tattacacac ccgattatcg ttttgttgac gtaattctta
      481 atggaaatta cataggtaat tatatgctta ccgatcaggt agaacgcgat aaagaccgtg
      541 tgaatactga aaaaatggat acttcacgcc ttgaccccaa cctgacagga ggctatgtgc
      601 tcgaaataga cggttttgga attaccggct cttacgatcc gagtaaaggt gagttgtttc
      661 ccgaagcctt ctccagtaac ataggcaaca aagtaaccat aaaatacccg gacaacgacg
      721 aaattactgc tgctcaacgt caatacatca tccaacatta caatgctttc gaacaaagca
      781 ttctaaacta caacaacacc caggttgcct acgaaaatat ttgccgttat ctggacacca
      841 ccgcctttgt caattggtac atcgccaacg aactgacagc caaccctgat ggagtctgga
      901 gtatttttat gaagaaacac cgggacgacg accgtcttta ttttggcccg ctttgggaca
      961 acgacatttc ctttggaaac tgcaaccgta tatattcgct tgcaaacgac ggccgctccg
     1021 aatccattat gttgaaatgt ttcagtaaca accgggtaaa ggagcttata aacaaagtgc
     1081 tgaccatttc tcccatcaga aaaatgatcg ctgaacgttg gaaagttatt tatcagaatg
     1141 agatgctcaa agatgatctg atacacctgc tctacaatat ggaagaagaa ctggatgctt
     1201 cgcaaaagct gaattaccaa aaatggagca ctgccctttc ttcgaggatt tacaacgaaa
     1261 tctattacgg atcaaatatt agcttcgcca tgcagatgga atatttgcgt aattacctca
     1321 gcgagcgaat tgatttcctc gacagttttt ttagcgccat ggccaacgaa ggtggcattg
     1381 atcccgaaga tcctcctctt cctccggtgg atacggcgac atatttcagc cccgagcctt
     1441 atgtatggta ccatctacaa tgccaactca acgataaata tttggtttgt caagagctag
     1501 ccgggggata tcagcttgat tatacttacg atccggaaac cgcgggcgat tgggcaaaat
     1561 ttgaaatcag ttgcagcaaa gacgatgaaa accgcagtgt ttactatctc cgcaataagg
     1621 ctgccaaaac ctatgtctat atcaatgctg ctaaggcagc aattcctctg gatccggtac
     1681 aaaaaacgtt ttttacgctt tatcccagcc caaacgaagc cgggcattac ggacttatgg
     1741 ttctttcgga caacagcact ctccatgaat tgggcatcga tgccaacggt agcgtagtct
     1801 cttggcctac cttgaacaaa aaaggtcaac gggaataccg gtttattccc gaccgctctc
     1861 atttccaaag cgtcttcgac ccaccgaatc cccaacaagc actatgggat atagaagaaa
     1921 attgggacaa caacattccc aatgcctacg ctacggccat tattgcagaa ggggcaaaac
     1981 tggaattgtc ccaagacgaa accgtaacag tagacagtct gattattttg cccggcgctg
     2041 aagtcttgaa ttacggcacc atccgggccg atgtcagcct gttttgctcc aacgacagcc
     2101 tgtcggcctc ttacgattac agtcaggaaa ctcaactgat ttccgaacgc gtgctttacc
     2161 ggagttttaa tcgcagccaa agttggtatg ccctatcctt gccactcgac gtaaaatcgg
     2221 tacgcaacaa agaaatgacc atattacaac ccggtaaaga ttattaccta atggaataca
     2281 aggccgactc caaccgtttt gattcactta gcctggcagg cagcttcaaa cccaaagcag
     2341 gcccttatct gttctcggtc agcccggaaa gagagcagca agcttttgta tttgtattcg
     2401 acaccaaaag ttcctaccaa accggaaaca gtgattacag ttttaccgga aacaccggtt
     2461 ttaaaaccat aaacatccct gcggccggta cctacctact ggaagatcat ttgcattttg
     2521 tactacagga gggtcaacaa ttattgctgc cggcctttca tgcttacttt tccgtcagcg
     2581 gagacagact gcccgattct ctggccattt acaacatgaa tccagccaac gacaccctga
     2641 ccctggagaa catatccaaa caggggccct tactagtgag cagagcaggt ttgttgatca
     2701 ttttcaacat ggaagccgga gaagattttt ccttgttcaa gctcgacggc agattgctcc
     2761 tcagagataa agctctgaac aatcccactt atgttcctct gaaacaaggg atttacctgc
     2821 ttcgatacaa aaaacgtttc cataaaatac atattccata ataacccata aaaatacaag
     2881 ctatgaaaaa aagaatcatt agtacattta tgctcacaac catcgttctg agcgtttcgg
     2941 cgcaaaaaaa cgagtggcag gatccgggta taaacgccgt gaacagagct gaaatgcaca
     3001 ccaattattt tgcttatgaa tcgcgggagg cagctctgaa aaactgtaga gagagctcca
     3061 gtaattatct cagccttaat ggtatgtgga aattctactg ggtcaaagac gctaataatc
     3121 gccctacgga ctttttccgt agcgatttcg acgaccgaaa ctgggccaat atgccggtgc
     3181 ccggtatctg ggaaatgaac ggctatggag atccggtcta tgtaaacaca ggctgggcct
     3241 ggcgcaatca attcagaagc aatcctcccg acatccctgt cgaaaacaat cacgtgggct
     3301 cctatcgccg ggaaatcact attcccgatc attggaaagg caaagaaatt tttgctcatt
     3361 tcggttccgt aacctccaac atctaccttt gggtaaacgg taaatacgta ggttacagcg
     3421 aagacagcaa gctcgaagcc gaatttaacc tgagtaagta tttgcgtccc ggcaaaaact
     3481 tgattgcttt tcaagtattt cgctggtgcg acggcaccta tctcgaagat caggactttt
     3541 ttcgcctgag cggcgtgggc cgtgattgct atctctacgc ccgcccttcg aattatatcc
     3601 aagacatcag gatcactccc gacctggacg cccaatacaa agacggcagc ctgaaaattg
     3661 acgtcagcat gaagggcaaa ggagaactgg aattgaatct gctggatgaa aacaatgaaa
     3721 ccctagccag tacacaacga aatgcaagcg gcaaattcag cctgacgatg aatctgagca
     3781 atccccaaaa atggtcggcc gaatgtccta atctctatac gttggtagcc actttaaaag
     3841 ggggcggccg ggtgctggag gctatccctg tcaaagtcgg tttccgtaaa atcgaactca
     3901 aaggtgatca gctattggtc aatggacagg ctgtattatt taagggggtc aaccgacacg
     3961 aaatggatcc tgactacggt tattatgttt ccccggaacg aatgatccag gacctcagat
     4021 taatgaaaga acacaacgtg aatgccgtac gcacctgtca ctatcccgac aacaatctgt
     4081 ggtacgatct ctgcgatgaa tacggtcttt atgtggtggc agaagccaac atcgaaagtc
     4141 acggtcttgg ctacggcgaa aggacaacag ccaagcgtcc cgattacgcg ctggcccata
     4201 tggagcgtaa tcagcgtaat gtgcaaagaa gctttaacca tccttcggtc attttttggt
     4261 ccttgggcaa cgaagcaggt tttggaccga atttcgaaat gtgttacaag tggattaaag
     4321 ccgaggatcc ctcccgtccg gtacaatacg aacaggccag gaccaacgaa tttacggata
     4381 ttttctgccc catgtattac ggttacaaca attgcatacg ttattgcgaa ggcgatattc
     4441 aaaaacccct gattcaatgc gagtacgtgc acgccatggg taattccgag ggaggcatca
     4501 aggagtactg ggacctggtg cgtaaatatc ccaaatacca gggcggtttc atctgggatt
     4561 tcgtagatca gtctctgcgg aagttcgaca aaaacggcgt tatgttttac gcctatggag
     4621 gagatttcaa cctatacgat gccagcgacg gcaatttctg cgacaacggc ctgatcagcc
     4681 ccgatcgcct gcctaatcct catttccacg aagttggcca tgtgtatcaa tccatttggg
     4741 ccagtgcctc cgatttggaa aagggagaaa tcgaaattta caacgaatac ttcttccgtg
     4801 atttgagcgc cttttacctt gaatgggcct tgctggtaaa cggtaaagtc tgccaaagcg
     4861 gtatggttga caagattgat atcgaagctc aacaaaccaa aaagctcaaa ctcgattacg
     4921 acttaagcgg catcagcccc gacaaggaag tattgctgaa tatttctttc aaactaagaa
     4981 aagcagaaca attattgccc gccggttttg tgttggccaa aaatcaactt tgcatacgcg
     5041 atcgctcaga gcaaagcttg aattttgata atatattgac tgccaacatc gagctacaga
     5101 ctcctaaagt aatcgaaaac gactggagat tccttatcat cgaagccgat gattaccgta
     5161 tcgaattcaa caagcacaac ggctacctg
//
LOCUS       DAOSGL010000151         5170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14291_length_5170_cov_3.964027, whole genome shotgun sequence.
ACCESSION   DAOSGL010000151 DAOSGL010000000
VERSION     DAOSGL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14291_length_5170_cov_3.964027"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..680
                     /locus_tag="PKY68_05670"
     CDS             84..680
                     /locus_tag="PKY68_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOH24559.1"
                     /translation="MLKVVFASHNKHKKQEVADILGNSVELIDLEDLNCSEDIPETQD
                     SLQGNALQKARFVHEKYGLNCFADDTGLEVEALNGAPGVHSARYAGPACNFEDNMDKL
                     LLELQGKENRRARFRTVVALLLDGREYFFEGRVEGEILRQKAGNKGFGYDPLFRPEGY
                     ELSFAQLDPEQKNRISHRAEAVQKMARFLETYKRNLQK"
     gene            739..3147
                     /locus_tag="PKY68_05675"
     CDS             739..3147
                     /locus_tag="PKY68_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24560.1"
                     /translation="MKKRLCLLLLSGLAGVLCFKLAAQPPVGEWRSHFSYRNATQVAA
                     TSDKVYVVANKHLFSYSPRDRFIEEYSVLTGLSGGQVQFIAQNDVSDCLLVVYTDGNM
                     DLLDDERVINLPDYKEKSLPADKSIYRLRMDGRYAYLCTGVGLLIIDVPKQEILETYN
                     LRSATVNYTAVFDFARIGDDYYMLTERGLYRGNAGDNLSDSKNWTAENFIPGKTPAQL
                     VSRKDSLYALVAGEGIYRYDGGWKAFYLSAGVLEISAQGPYLLALTAGLPLVFAADNS
                     QLTLHDSDVSGEYSLYEYNAQMQAGLKQMYGVALDEKLNRIYTTAGSDGLLEWIYYAE
                     AEAYMVSNSKILPNGPGVDTAWDLYFRNNDLYVASGGRWGDRYKFTGDIMRFRDNNWV
                     SLINRDSIERKTGYPFQDILNVAVDPADEEHFFATSWGEGLYEFKGGKLDTLHSYANS
                     PFLPAWDGLPYRYVRVDGAVFDAGGNLWVLNSAYPAHQASGSGGLHILMRESGEWFSP
                     GYADLKPAPTLNKIFFTGNHMVWINAERAIYGIFVLDYNGSLEDTSDDRTRWISSFTD
                     QDGINLNVFTVHCITEDLNNRLWIGTNMGPLMLASYRSVFDETPVFFRVKIPRDDGTN
                     EADYLLGNSRVYCIAVDGANRKWMGTRNDGLYLLSPDGTETIHHFTKENSPLPSNEVW
                     SVAIHPQSGEVFIGTAGGLVSYRSDASPAAPDYSEVYAFPNPVHPGYRGKITVKGLTE
                     NSQVRITDLSGNLIISGSSLGGQFGWDGNNARGQRVSSGVYLVFAADNQGEKGVVCKI
                     VIIN"
     gene            3276..>5170
                     /locus_tag="PKY68_05680"
     CDS             3276..>5170
                     /locus_tag="PKY68_05680"
                     /inference="COORDINATES: protein motif:HMM:NF027701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24561.1"
                     /translation="MKFNLRVRKLFPLVLLLLLNNYACQNKMELYYQEPDWLKGSIYE
                     VLQERGDYSIFLKGIELAGFKSIVNGKSILTVMAPTDSAFNAYLEEHYGGKSIEELPR
                     AELKKLIGFHILYYAFDKEKLINFRPGEGDGATKEEKLKNAGLYYKHRTKSQDGLSLE
                     IDTAGAEVSVYHMERFLPVFSYRMFQTKQIDAKYNYEYFFPTTAWRDDAGFNVSNAAV
                     TEYSVLADNGYVYLVDRVLKPLETIYAELASNEDYSDFLELYDKYEYYQLDENLTLEY
                     GDGTDLYQHYHTSPLPNIACEWPVTDYKKVTDLAFVSYSVFAPSNEAFRGFFREYWEV
                     GGYDSLSEVSKQSIEYLLYNSVYSSSMVFPEEITKGLIENSYGTVIKFDVDRIPQANR
                     KICVNGSFYGCSELTPPAMFGSVTGPAFQYKKYSYFLKMLNTADLILTLCSDATSYFM
                     LYPSNELMNQDGITQVEEVLYRGSNKLSNSSQQNYVYAHVLSQDAITGSYRDLLTDAG
                     TGNHVFRTLSPGMYLYWYMKDGKITNSVKFTELLYNPALTEDQLFCSLQELSFRDGWT
                     NGKCYAYQNDVPYLFEGSMENAIYSKFVPMMIGNRNNSATIYHGFVQLLELSGLLDFD
                     SQSVLPVV"
BASE COUNT         1341 a         1179 c         1290 g         1360 t
ORIGIN      
        1 gtggaagagc aattgcgccg gcaagccctc aggtctatcc aacgaatgct cgacatttcg
       61 gcaaacctca aataaatttc cgtatgttga aagttgtatt tgccagccat aacaagcaca
      121 agaaacagga agtggccgat attctgggga attccgtcga gttgatcgat ctggaggatt
      181 tgaattgcag cgaagatatt cccgaaactc aggatagtct gcagggcaac gctttacaaa
      241 aggcccgttt tgtacacgaa aaatacggtc tgaattgctt tgccgacgac accggtcttg
      301 aagtggaagc cctgaacgga gcaccgggag tccattcggc ccgttatgcc ggccccgcct
      361 gcaattttga agataatatg gataagttgc tcttggaatt gcagggcaaa gaaaaccgca
      421 gagcacgttt cagaactgtg gtggccttgt tgctcgatgg ccgggaatat tttttcgaag
      481 gccgggtcga aggcgaaatt ttgaggcaaa aggcgggtaa caaaggtttt ggttatgatc
      541 ctttgtttcg ccccgagggt tatgaattga gttttgccca actggatccg gaacaaaaaa
      601 acaggatcag ccatagggca gaggctgtgc aaaaaatggc ccgttttttg gaaacctata
      661 aaagaaacct acaaaaataa acaagataaa ccccggttct gtacaatatt cgacccttgt
      721 ttgcggttat tgcttgtgat gaaaaagaga ctctgtttat tgctgttgtc cggacttgcc
      781 ggtgtgctgt gctttaaact tgcagcccag ccgcccgttg gagaatggcg cagccatttt
      841 tcgtaccgga atgccactca ggtggccgct acctccgaca aagtatacgt ggtggccaac
      901 aaacatttgt tttcgtattc gccccgggat cgttttatcg aagaatattc cgttttgacc
      961 ggacttagcg gaggccaggt gcagtttata gcccaaaacg atgttagtga ttgtttgctg
     1021 gtggtataca ccgacggcaa catggattta ttggacgacg aaagggtgat caatctgccg
     1081 gattacaagg aaaaaagcct gcccgccgac aaaagcatct accggcttcg catggacggc
     1141 aggtatgcct atctgtgtac cggagtcggc ttgttgataa ttgatgtacc taagcaggaa
     1201 attctggaaa cgtacaacct gcgaagtgct acggtaaatt atacggcggt gttcgacttt
     1261 gcgcgtatcg gcgacgatta ttatatgctc accgaacgag gcctttaccg gggaaatgcc
     1321 ggcgacaacc tgtcggacag caaaaactgg acagcagaga attttatacc gggtaaaacc
     1381 cctgcacaac tcgtttcgcg caaagattcc ctttatgcat tggtggccgg agagggcatt
     1441 tatcgctacg atggaggctg gaaagcattt tatttaagtg ccggtgtgct ggaaatatcg
     1501 gcacagggcc catatttgct ggctctgacc gctgggctgc ctttggtttt tgcagcggac
     1561 aacagtcagt taactctcca cgacagcgat gtgtcgggag aatattcttt gtatgaatac
     1621 aatgcacaga tgcaagccgg gctgaagcaa atgtatggcg ttgccctgga cgaaaaactt
     1681 aatagaattt ataccacggc cggcagtgac ggccttctcg aatggattta ttacgccgag
     1741 gcggaggcct atatggtgag taacagtaaa atcctgccta acggcccggg tgtggacaca
     1801 gcctgggatt tgtatttccg caacaacgat ttgtatgttg ccagtggcgg gcgttggggc
     1861 gaccggtaca aattcaccgg agatattatg cgttttcgcg acaacaattg ggtttccctg
     1921 atcaaccgcg acagtattga acgcaagacg ggctatccct ttcaggatat tttgaatgta
     1981 gcggtagatc ctgccgatga agaacatttt tttgccacct cttggggcga aggcctgtat
     2041 gaattcaaag ggggaaaact cgatactttg cattcgtacg ccaacagccc ctttttgcct
     2101 gcctgggacg gattacccta tcgttatgtc cgggttgacg gagctgtttt cgatgccgga
     2161 ggcaatctct gggtgctcaa ttcggcttat cccgcacatc aggcttcggg ttccggaggc
     2221 ttgcacatct taatgagaga aagcggagaa tggttctcgc ccggctatgc cgatttaaag
     2281 cctgccccca cactgaataa gatttttttc accggcaacc atatggtctg gatcaacgcc
     2341 gagagagcga tctatggcat ttttgtactc gattacaatg gcagtctcga agatacttcc
     2401 gacgaccgga cccgttggat ttcgtccttt accgatcagg acgggataaa cctgaatgta
     2461 tttacggttc actgcatcac ggaggacctc aacaacagac tttggatcgg caccaatatg
     2521 ggcccgttga tgctcgcatc gtatcggagt gtttttgatg aaactccggt ctttttccgt
     2581 gtaaagattc ctcgcgacga cggtaccaac gaagccgatt acctgctggg caacagccgg
     2641 gtctattgca tagccgtgga cggggccaac cgcaaatgga tggggacccg caacgacgga
     2701 ctttatctcc tgtcgcccga cggcacggaa accatacatc attttaccaa agaaaacagt
     2761 cccctgcctt cgaacgaagt ctggtctgtt gccattcatc cccaaagcgg agaggtattc
     2821 atcggcacag ccggagggct ggtgtcgtac cgtagcgatg cttcgcctgc cgcccccgat
     2881 tatagcgagg tttatgcttt tcccaatccg gtgcaccccg gttacagagg taaaattacc
     2941 gtgaaaggcc tgacggaaaa ttcccaggtg cgaattaccg atttaagtgg gaatttaatc
     3001 atctccggtt cttccctggg aggacaattc ggctgggacg gcaacaatgc cagggggcaa
     3061 agagtgtcga gcggggttta tcttgtgttt gccgccgaca atcagggtga aaaaggagtt
     3121 gtgtgtaaaa tcgtaataat caattgaaca catgctctaa gtcgtagcgg cgtagtttaa
     3181 catttttagt cgcgggtttg tttttttttc atatatttgt gccgctttgc agttttttcg
     3241 tatgaccttt atataattgt aatacctaaa ttatcatgaa attcaatctc cgtgtacgga
     3301 agttgttccc acttgtattg cttttactgt taaacaatta cgcctgccag aataaaatgg
     3361 agctctatta tcaggaaccc gactggctca agggaagtat ttatgaggtg ctgcaggaac
     3421 ggggggatta ttccattttt cttaaaggaa ttgagctggc cggtttcaag tcgatagtga
     3481 acggcaaaag cattctcacg gtgatggctc ccaccgacag cgcattcaac gcctacctcg
     3541 aagagcatta cggggggaaa agcatagaag aattaccccg ggccgaattg aaaaagctca
     3601 taggttttca tattctatac tatgccttcg acaaggaaaa actgattaat ttcaggccag
     3661 gtgaaggcga cggcgccacc aaagaagaga aactcaaaaa tgccggcttg tattataagc
     3721 acagaactaa aagccaggac ggtttaagtt tggaaataga cacagccggt gctgaagtca
     3781 gcgtgtatca catggagcgt tttttgccgg tattttcgta ccggatgttt caaaccaaac
     3841 aaatcgatgc caaatacaac tacgaatact tttttccgac cactgcctgg agagacgatg
     3901 ccggtttcaa cgtttcgaat gctgccgtaa cggaatattc ggtcctggca gacaacggtt
     3961 atgtttatct ggtggacagg gtgcttaaac ccctcgagac catttatgca gaacttgctt
     4021 ccaacgagga ctattccgat tttttggaat tgtacgataa atatgagtat tatcagctgg
     4081 acgagaacct gaccctcgaa tatggggacg ggactgatct ttaccagcat taccatacct
     4141 ctcctttgcc caatatagcc tgcgagtggc ccgtaaccga ttacaaaaag gtaacggatc
     4201 tggcctttgt ttcttatagc gtgtttgctc ccagtaacga ggctttcaga ggttttttca
     4261 gggaatattg ggaggtcggc ggctacgatt ccctgagcga agtaagcaaa cagtccatcg
     4321 aatatttatt gtataatagc gtatattctt cttcgatggt attccccgaa gaaattacca
     4381 aaggcttaat cgaaaacagt tacggtaccg ttattaaatt tgatgttgac cggattccgc
     4441 aggccaatcg taaaatttgt gtcaacggct ctttttacgg ctgcagtgaa ctcactccgc
     4501 cggccatgtt cggttctgtg acgggtccgg cttttcaata caagaaatac agctatttcc
     4561 tgaaaatgct caacaccgcc gacctgattc ttacgctttg ttcggatgcc accagttatt
     4621 ttatgcttta tccctcgaac gagctgatga accaagatgg tattactcag gtggaagaag
     4681 tcttgtatag aggatccaat aaactctcca actcttctca gcaaaattat gtttacgccc
     4741 acgttcttag ccaggacgcc atcacgggct cctaccggga tttgttgacg gatgcgggaa
     4801 cgggcaatca cgtatttcga accttgtcgc cgggtatgta cctttattgg tatatgaaag
     4861 acggcaagat taccaacagt gtgaaattca cagagttact gtataacccc gccctcaccg
     4921 aagatcaact tttttgcagc ctgcaagagt tgagtttcag agatggttgg accaacggca
     4981 aatgctatgc ctatcaaaac gatgtgccct atctgtttga aggttccatg gaaaacgcca
     5041 tctattccaa atttgtaccc atgatgatag gaaatagaaa caactctgca accatttatc
     5101 acggttttgt gcagttgctc gagttgtccg gcctgctcga tttcgattct caatcggtgt
     5161 tgccggtggt
//
LOCUS       DAOSGL010000152         5063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14709_length_5063_cov_3.311901, whole genome shotgun sequence.
ACCESSION   DAOSGL010000152 DAOSGL010000000
VERSION     DAOSGL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14709_length_5063_cov_3.311901"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKY68_05685"
     CDS             complement(<1..820)
                     /locus_tag="PKY68_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24562.1"
                     /translation="MNLMKEFKNPFRLLMRLSVVFGLVFLVSCKDDYIYDEKSPDWLG
                     ESIYDYLKQDGHFDYYVKIIEDVGQKEVLAKTGSKTLFVTPDSIFDRFFQDNVWGLKS
                     FEEMNLSQKRQLLFFSMIDNAYLIETLSNYYSGGVLNEGQAMRRLTGLSVLDSVPFIE
                     GNKLPKATIWNRWRTKGMYLLKDNSTKPMVHFLQKYLENVGISDQDFKVMTGADRSYN
                     DAFIFQHKVIKRDIVCKNGYVNVLDGVMIPRDNMAEYIRSNENTTQFSELLDRFC"
     gene            1233..>5063
                     /locus_tag="PKY68_05690"
     CDS             1233..>5063
                     /locus_tag="PKY68_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOH24563.1"
                     /translation="MKKNYLKLATRTCLLAITCMLATSALADITSNLHLHYTFDGLTA
                     EQSEVPDVSGNNNAGTRMGGAYYVEGKFGDALRLETVNDYVYMPDGFMVGMEDITIAG
                     WIYKDAANGWDRLVDFATNTDTNMFITTGGPGYVRFAIKNIQVRPAGEEHINGKALTF
                     GRWNHIAVTIKYTNNIGLGILYVNGVEVGRNNNITLKPSDLGFTTKNYVGKSVYTADP
                     SVVGNIDDLRIYKRALTAEDIAELLGVPDEITDCYNNLALDLPEDYEFTGLTADLTLP
                     TTWGDSAVNISWTSSDTLLIDNEGKIVAFPEKYQDACILTASLSQMRGEEEFTAERTF
                     IVKVYPQETEINPIIAVWDFTEETLGTDEDGNVTVTDKSGNDFVGTCLGGARIVTMGE
                     SNPVSAMYIEQAGAYFDMGQEIGKAVAGLTDYTISVFYRKDSVGGVTWSSSAYGQWLY
                     CFCNSNDLASDGQGGIFYEPYRAYHAVFTTNYGDETGVQRGQRRTPIGTWHNITYVQS
                     GQKGTLYFDGEVAGSSDNVYIPAYALKAKGHRGTIHNYIGLPCYSGDPKLSKTHIAGL
                     QIYGVALTQEDLQSEELTNISARIAALNTAYNENPEVVGPEFQQEYDALTLPDISALT
                     EETVLTLPTQGTIEPAIKITWVSSQPEYVSNTGVVTQPKYFDKKIVLTATLNQGTTFK
                     SKTFEGKVLVKGGFTTDLVANFDFSKGSGTTVTDQGEFAFEGTLHEEAYIATIGEGDN
                     AIKVLYLANGDGYFDMGKEIGKAVSGLTDYTVSVFFRKDNNQGELSQFTGYGQWLYGF
                     ANTNNLGGQAYGAMYYEPLRDRHVCTPDNYNAEANNHVGVGQGNTFVGSWHNITFTQN
                     GDTGVLYKDGWAAATAKISNPETALWRSGLSGTIHNYIGKPCYSGDPYLQNTMIASLV
                     IYKKGMTEEEVNTLFDIENTIVKLDAAYALNDGSASVRAIDLQNLVDEAKDYAALAYP
                     GLDALNEAITAAEAAIASNNVTEEDIDNLKTAIRMYRFTQPASGAAPADFTFAIENPS
                     FEGTFGGKLDPNSIVEGDGSYKYPTGWTLYMDKTGWCNAVDITAGPSDGTKAFETWAA
                     TIRKFDIYQDIFLPAGNYILSAEVRTNAAPPYTQHTYVAIKGDKTYSSVSLDPERLVT
                     GSGWNAVTNFQTLSTSFALNADGNVRIGLASEGFMQFDNFRLTCFGADEPATAEAPAD
                     YTFAIANPSFEDGLGGTLDTTSVLGSGNHNVPAGWNLYLNQSGWCNAVSITDAPSDGA
                     SAFETWAATINRIDL"
BASE COUNT         1440 a         1258 c         1115 g         1250 t
ORIGIN      
        1 cacaaaaacg atcaagcaat tccgaaaact gagtagtgtt ttcgttgctc cggatgtatt
       61 ccgccatatt gtcgcgagga atcatcactc catcgagcac attcacataa ccgtttttac
      121 atacgatatc ccttttaatg accttatgtt gaaagatgaa agcgtcgttg taactgcgat
      181 cggcacctgt cataaccttg aaatcctggt cactgatgcc cacgttttcc aggtactttt
      241 gcaaaaaatg aaccatcggt ttggtggaat tgtcttttaa caggtacatt cccttagttc
      301 tccaacgatt ccatattgta gctttcggta atttattgcc ctcaataaaa ggcacgctgt
      361 ccaatacgga caatccggtc agacgacgca ttgcctggcc ttcgttcaga acaccgccgc
      421 tgtaatagtt cgaaagagtt tcaatcaaat acgcattatc tatcatcgag aaaaacaaca
      481 attgtcgttt ctgggacaga ttcatttcct cgaaactttt caatccccat acattgtcct
      541 gaaaaaagcg gtcaaaaata ctgtcggggg taacaaacag ggttttactg ccggttttgg
      601 ccaatacttc tttctgaccg acatcctcga ttattttgac ataataatca aaatgaccgt
      661 cctgtttcag gtaatcgtag atgctttcgc ccaaccagtc ggggcttttt tcatcgtaga
      721 tataatcgtc cttgcacgat acaagaaaca ctaatccgaa tacgacactt aatcgcatca
      781 gtagtcgaaa gggattttta aattctttca tcagattcat atgggtttgg atttatttag
      841 aaagtcaact gcaacgaatt tcatactatg aatttcataa gtttttcaaa aatttagtca
      901 caaatatatg ccataacatc agcatcaggg tggatgattc attcaaataa gtggacaaga
      961 aaataaaatc aagtttttta acaagcattt tgtccaccaa ttataaacat ttatccacca
     1021 aaaatagaca caatacgtat atttttgcga agcgtgttta caaaatcagg aggtggagaa
     1081 gataaaaaac aaaaaaatga aaataaattc ctgtccactc taattattgt actgctcgca
     1141 agtgttattt tacttttgta tattgtctta atttttctta tttcgcaaat tctttttacc
     1201 tttcaacacc actaacttta aactttatga aaatgaaaaa aaactacctc aaattggcaa
     1261 ccaggacttg cctgcttgcc attacttgta tgcttgcaac ttcagcattg gctgatatta
     1321 caagcaacct gcatctgcat tatactttcg atgggcttac tgcggagcag tccgaagtac
     1381 ccgacgtttc cggtaacaac aacgcaggaa cacgtatggg aggcgcctac tatgtcgaag
     1441 gtaaattcgg tgatgctctc cgtcttgaaa ccgtgaacga ctatgtttat atgcccgacg
     1501 gttttatggt ggggatggag gacattacca ttgccggctg gatatacaaa gatgccgcca
     1561 atggttggga ccgcctggta gactttgcta ccaacaccga caccaacatg tttattacca
     1621 caggaggacc gggttacgtg cgttttgcca tcaaaaacat tcaggttcgt cctgcagggg
     1681 aggaacacat caatggcaaa gctctcacct ttggccgctg gaatcacatt gcggttacca
     1741 tcaaatacac caacaacatc ggtttgggta ttttatatgt taacggtgtc gaagtaggtc
     1801 gtaacaacaa cattacactg aagcccagcg atcttggatt tacgacgaaa aactacgttg
     1861 ggaagtcggt ttacactgct gacccctcag tagtgggaaa catagacgat ttgcgcattt
     1921 acaaacgtgc cctcactgct gaagacattg ccgagttact tggcgtaccc gacgaaatta
     1981 cggattgcta caacaatcta gcgttggatc ttccagaaga ttacgaattt accggcttaa
     2041 ccgcagattt gaccctgcct accacctggg gcgacagcgc tgtaaacatc agctggacat
     2101 cttccgacac cttgctgatc gacaacgaag gcaaaattgt ggctttcccc gaaaaatacc
     2161 aggatgcctg tatattgacg gcttccttgt cacaaatgag aggcgaggaa gaatttaccg
     2221 ccgaacgcac tttcatcgta aaggtttatc cccaggaaac agaaatcaat cctatcattg
     2281 ccgtctggga ctttacggaa gaaaccctgg gtaccgatga agacggaaat gtaaccgtaa
     2341 cggataagag tggaaacgat tttgtgggaa cctgtctggg tggagcccgt atcgtcacca
     2401 tgggcgaaag caatccggtc agcgccatgt atatcgaaca agccggtgct tactttgata
     2461 tgggccaaga aataggtaaa gccgtagccg gtttaaccga ctacaccatc agtgtgtttt
     2521 accgtaaaga cagcgtaggt ggcgtcacct ggagcagcag cgcctacggt caatggttgt
     2581 attgcttctg taacagcaac gacctggcca gcgatggaca ggggggtata ttctacgaac
     2641 cctatcgcgc ctaccacgcc gtattcacca ccaactacgg cgacgagact ggcgttcaaa
     2701 gaggacaaag gagaactcct ataggcacct ggcataacat tacttacgta caaagcggcc
     2761 aaaaaggcac tctgtatttt gacggcgagg tggccggaag cagcgacaat gtttacattc
     2821 ccgcctatgc tctcaaagct aagggacaca gaggcaccat tcacaattac atcggccttc
     2881 cctgttattc cggcgacccc aaattgtcca aaactcatat tgccggcttg cagatatacg
     2941 gagttgctct tacccaggaa gacctgcaga gcgaagaact gaccaatatc agtgcaagaa
     3001 tcgctgccct gaacaccgct tacaacgaga atcccgaagt ggtaggtccg gaattccagc
     3061 aagaatacga tgcgctgacc ttgcctgaca tcagtgcatt aaccgaagaa acggttctta
     3121 cccttcccac ccagggaacc atagagcccg ccatcaaaat cacttgggta tccagtcagc
     3181 cggagtatgt ttccaacacg ggagtagtta cacaacccaa atactttgat aaaaagattg
     3241 tactgactgc cacattgaac caaggtacca ccttcaagag caaaactttc gaaggcaaag
     3301 tactggttaa gggcggattt acaaccgatc ttgttgccaa tttcgacttc tccaagggca
     3361 gtggtaccac cgtcacggat cagggtgaat ttgcctttga aggaaccctg cacgaagaag
     3421 cttatattgc taccatcggc gaaggcgaca acgccatcaa agtgctctat ttggccaacg
     3481 gcgacggcta cttcgacatg ggcaaagaaa tcggcaaagc cgtttcgggt ctgaccgatt
     3541 acaccgtcag tgttttcttc cgtaaggaca ataaccaggg agaattgtca cagtttaccg
     3601 gctacggaca atggttgtac ggattcgcta acaccaacaa cctgggcggc caggcttacg
     3661 gcgccatgta ttacgaaccg ctgcgcgacc gccatgtttg tacacccgat aattacaatg
     3721 cagaagccaa caatcatgtg ggcgttggcc aaggcaacac ctttgtagga agctggcaca
     3781 acattacctt tacacaaaac ggcgacaccg gagtgcttta caaagacggt tgggcagctg
     3841 ccacggccaa aatcagtaat cctgaaaccg ccttgtggag gagtggactg agcggaacca
     3901 tccacaatta catcggtaaa ccctgttatt cgggcgaccc ctacctgcag aatactatga
     3961 ttgcctcttt ggttatctac aaaaaaggta tgaccgagga agaagtcaat accctatttg
     4021 acatcgaaaa caccatcgtg aaactggatg ccgcctatgc cctgaacgac gggtcggcca
     4081 gcgtacgggc catcgatctt cagaatttgg tagacgaagc caaagattat gccgctttgg
     4141 cttaccccgg acttgatgct ctcaatgagg ccattaccgc tgccgaagca gccatagcaa
     4201 gcaacaacgt caccgaagaa gacatagata acctcaagac cgccatcaga atgtatcgtt
     4261 tcactcaacc ggcttcgggt gcagctcctg cagacttcac ttttgccatt gagaacccga
     4321 gcttcgaagg aacctttggc ggaaaacttg atcccaacag catagtcgaa ggcgacggca
     4381 gctataaata tcccacaggt tggaccttgt acatggataa gaccggctgg tgcaatgccg
     4441 ttgacattac tgccggtccc tccgacggaa ccaaggcttt cgaaacctgg gcagcaacca
     4501 tacggaagtt tgatatttat caggacatct tcctgcctgc aggcaactac atcctgtctg
     4561 ccgaggtcag aaccaacgct gcgcctcctt acactcaaca tacctatgtt gccatcaagg
     4621 gcgataagac ctattcttcg gtcagcctgg atcccgaaag attagttacc ggaagcgggt
     4681 ggaacgccgt aaccaatttc caaaccctga gcaccagctt tgctcttaat gccgacggca
     4741 acgtccgcat cggactggct tcggaaggct ttatgcaatt cgacaacttc aggcttactt
     4801 gctttggtgc cgatgagccc gccaccgcag aggctcctgc agattatact tttgctatcg
     4861 ccaatccgag cttcgaagat ggtttgggag gcacactcga tactaccagt gttctcggta
     4921 gtggaaatca caacgtcccc gcgggttgga atctgtacct gaatcaatca ggctggtgca
     4981 atgccgtcag tattaccgat gcaccctccg acggagccag tgcatttgaa acttgggcag
     5041 ccaccatcaa caggattgat ctt
//
LOCUS       DAOSGL010000153         5061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14718_length_5061_cov_3.764882, whole genome shotgun sequence.
ACCESSION   DAOSGL010000153 DAOSGL010000000
VERSION     DAOSGL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14718_length_5061_cov_3.764882"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKY68_05695"
     CDS             <1..603
                     /locus_tag="PKY68_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH24564.1"
                     /translation="TTTEYTIPDLTIGNTYTLGVQSVYGSDTSEIVTTSFTVYETYTV
                     TFAVVDAEDSPVEGANIVIDEDMLTTDSSGVASIDLAGGDYTYTVSKPGYADFTGSFT
                     VTDTVQTIHVNLTTGMDKLATGSVRLYPNPVKNTLIIEHNTGDEFVIELYSISGLLVG
                     TTKAVSRTTAVDVGALSSGSYFVRIIGTNSAPTMHRFIKQ"
     gene            complement(795..1751)
                     /locus_tag="PKY68_05700"
     CDS             complement(795..1751)
                     /locus_tag="PKY68_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOH24565.1"
                     /translation="MEQSNRTIAEKCLRAEAQAILNLIPQLDDQFDAAVDLILNCNGK
                     VIVTGVGKSGHIGAKIAATLSSTGTPAFFTNPLDVYHGDLGAITADDVVIAISQSGHT
                     DELLRFIPFLLEEKIPLIGITSNPGSLLARHSTFHIHLGVSEEACPLNLAPTSSTTAT
                     LAMGDALACVLIERRKFEARDFARFHPGGSLGRRLLTCASDVMRSEDLPTMSESLPLG
                     EAIIRVSEGRLGLCVALDEQERIIGLITDGDVRRAMQNLRERFMETPVSEVMTRKPKC
                     VTPETKIREIQDIMNRNKIHSVLVADEKQRLLGVVDHFMCML"
     gene            complement(1755..2507)
                     /gene="kdsA"
                     /locus_tag="PKY68_05705"
     CDS             complement(1755..2507)
                     /gene="kdsA"
                     /locus_tag="PKY68_05705"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOH24566.1"
                     /translation="MKPLFIAGPCVIESFEVLDEVAATLKRISIAYDIDVYFKASFDK
                     ANRTSIHSYRGVGMERGLQMLADVKARHGLKLLTDIHESHQAAPVGEVADAVQIPAFL
                     CRQTDLLVAAARTGLPVNIKKAQFLSGLDMQYPVEKCREAGASDVWLTERGNTFGYNN
                     LVVDFRNIPDMKQFTSRVIMDCTHSVQRPGAAGGKTGGNREYVPAMALAARAFGATGY
                     FFEVHPNPEKALSDGPNMLFLRDFERIVKSLI"
     gene            2668..4350
                     /locus_tag="PKY68_05710"
     CDS             2668..4350
                     /locus_tag="PKY68_05710"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOH24567.1"
                     /translation="MLTDLEIAKKTPLLPIAKIAEKIGILGDELESYGTYSGKIKLSI
                     LERLKNQPDGKLILVSAMSPTSFGEGKTLTSIGLGQALNRLGKKAIVAIREPSLGPVF
                     GMKGGAAGGGYSQVLPMELINLQFNGDFSAIATAHNLLAAMIDNHIIKGNELNIDLNR
                     ILWKRTMDMNDRALRNIVVGLGGSANGVPRETGFVITAASEIMAILALSKSRADLKRR
                     LGEIVVAYDNKQQLIRARDLEAHRAMAVILNDAIMPNLVQTTENTPALVHAGPFANIA
                     HGTNSVLADKIALKLSDYVVTECGFGTELGGEKFFNIVCRDNPIWPSVVVIVATCRAI
                     KFHGGVPQKPASALQEENLEAFAKGLKNLEVHIRNMKKFGVPVLVAINRFPFDTAKEI
                     EMIEKLCAELKVESAPHEAFAKGGAGTIELAEKTIHIVEQYKNPQKKFLYSVDMPIEE
                     KIRTIATQMYGAKDIIISPKIRKKLDRFLEFGFGNLPVCIAKTQMSLSDDPNMIGAPK
                     GWVLTVSDVNLSAGAGFLVAVCGNMMLMPGLPKVPAAVNMDVDDDGEITGLF"
     gene            4443..4871
                     /locus_tag="PKY68_05715"
     CDS             4443..4871
                     /locus_tag="PKY68_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOH24568.1"
                     /translation="MKKNLLIIILLLFTSTWSIGQEAPDKSGPKEIVKTLKASNLSDI
                     QARQFVESPPTGETFYEGFEADVFPPAGWKVINDGSSDQTWVRYTEEPITGNASASIR
                     YSSQAHDDWLITPPLAPVDGNNVISFRAKQRTGTYIDKLM"
BASE COUNT         1393 a         1217 c         1167 g         1284 t
ORIGIN      
        1 acaacaaccg aatacacgat tcccgacctg actatcggca atacttatac tctgggtgtt
       61 cagtcggttt acggctcaga tacgtctgaa atcgttacta cttcttttac cgtttatgaa
      121 acgtacaccg taaccttcgc cgttgttgat gcagaagaca gcccggtgga gggtgcgaat
      181 attgtaattg acgaggatat gttgactacc gattcttcgg gtgtggcaag cattgactta
      241 gccggcggcg actatactta tacggtatcg aaaccgggat acgccgactt tacgggttcg
      301 tttaccgtta ctgataccgt tcagacaatc catgtgaacc tgactaccgg aatggataag
      361 ttggcgacag gttccgtacg actctatccc aaccccgtta aaaacacctt gataatagaa
      421 cacaataccg gcgatgaatt tgttatagaa ctatacagca tcagcggact gttggtaggt
      481 acaacaaaag ccgtaagccg gacaaccgcc gtcgatgtcg gagcgttaag ttcaggttct
      541 tattttgtga gaatcatcgg aaccaacagc gctcctacaa tgcacagatt tattaaacaa
      601 taatccggtg cgagctaaag aaaaaggagc tgtctcgttt gagacagctc ctttttcttt
      661 gcccgacgcg aacattctct tcatagtatt ctacgttcgc ttgttcagcg aaacgtcggc
      721 ctgccacttc actcgatgat tgcatcgccg atatcgaaaa acaaagctct aaaaaaacga
      781 gaaagaggga ggtttcagag catacacatg aaatggtcca ccacaccaag caacctctgc
      841 ttctcgtctg ccaccaaaac ggaatgaatc ttgtttcgat tcatgatgtc ctggatttcc
      901 cgtatcttcg tttccggggt tacacatttc ggtttacggg tcatcacttc gcttacgggc
      961 gtttccataa atcgctcacg caagttttgc atggcacggc gcacatcacc gtccgtaatc
     1021 aggcctatga tgcgttcttg ctcatcgagt gccacacata atcccaagcg cccttcgctc
     1081 acacgaataa tggcttctcc caacgggagg gattcagaca tcgtaggtaa atcctcacta
     1141 cgcattacgt cgcttgcaca ggtaagtaag cgacgcccca gcgatcctcc gggatgaaaa
     1201 cgggcaaagt cacgggcctc gaatttcctc cgctcaatga ggacacaagc caatgcatcg
     1261 cccatggcca gtgttgctgt ggtgctgctg gtcggtgcca aattcaaggg acaggcctct
     1321 tcggacactc ccaaatggat gtgaaaagta ctgtggcgcg ctaaaagaga acccggattg
     1381 cttgtgatgc caataagtgg aatcttttcc tccaaaagaa aaggtatgaa gcggagcaac
     1441 tcatcggtat gtccgctttg agagatggca atcaccacat cgtcggccgt tatcgcacct
     1501 aaatctccgt ggtacacatc gagcggattg gtaaaaaaag ccggtgtacc cgtgctactt
     1561 agcgtagccg ctatttttgc accgatgtgc ccacttttac caacacctgt gactatcacc
     1621 ttgccattgc aattcaaaat caagtccacc gcagcatcaa actggtcgtc gagctgcggt
     1681 attaaattca agatggcctg tgcttcagca cgcaggcact tctcggcaat tgttctgtta
     1741 ctctgttcca tgtgtcaaat caaagactta acaatccttt caaaatccct caaaaataac
     1801 atgttcggcc cgtcactcaa tgccttctcc gggttgggat gtacctcaaa gaaatacccg
     1861 gtcgccccaa aggcccgagc agcaagcgcc attgcgggca catactcacg attcccccct
     1921 gtcttccctc cggctgcccc cggccgttgc acactgtggg tgcagtccat gattacacgg
     1981 gacgtgaatt gtttcatgtc agggatgtta cgaaagtcca ccacaagatt gttgtagcca
     2041 aaggtattgc cccgttctgt aagccacaca tcacttgctc cggcctcgcg gcatttctcc
     2101 accggatatt gcatatccaa tcccgaaagg aactgtgcct tcttaatatt cacgggtaaa
     2161 cctgttcggg cagcagctac cagcaaatcg gtctgacggc aaaggaaggc gggaatctgc
     2221 accgcatctg ccacttctcc caccggggca gcctggtggc tttcgtggat atccgtaagt
     2281 aacttcagac cgtgcctggc cttgacatcg gcgagcatct gcaatccgcg ttccatccct
     2341 actccccgat aagaatggat actcgtgcgg tttgccttgt caaaggatgc tttgaaatat
     2401 acatctatat cgtaggctat gctgatgcgc ttcaaggtcg cggccacttc gtcaagcacc
     2461 tcaaaagact ctattacgca agggccggct atgaataatg gcttcatttt ctgattttag
     2521 gttggttgct cgttatcacg aggttgcgaa ggtagcgctt ttaaattaaa ccccatagaa
     2581 attccgaaaa aagtccatac gtttagtgtt gctattaaaa aaataattac gtaaattcgg
     2641 gttctataat gccaaaataa aaccgccatg ttgacagacc tggaaatcgc aaaaaaaaca
     2701 cctttgttac ctattgccaa aatagctgaa aaaattggca tactcggaga tgagctggaa
     2761 tcgtacggca cctattccgg aaaaatcaaa ttaagcatat tggaacgact caagaatcaa
     2821 cccgatggga aattgatttt ggtgtctgcc atgagtccga ctagtttcgg agaaggtaag
     2881 acgctgactt caatcggatt gggacaagcc ttgaaccgac tgggtaagaa agctattgtg
     2941 gccatacgcg aaccctcgct gggtcctgtt ttcggtatga aagggggagc tgccggcggc
     3001 ggatattctc aagtgcttcc catggaactg atcaacctgc aattcaatgg tgatttcagt
     3061 gctattgcta cggctcacaa cctgctggcc gccatgatcg acaaccacat tatcaaaggc
     3121 aacgaactca acattgatct gaaccgcatc ctgtggaaaa gaaccatgga tatgaacgac
     3181 agggctttac gaaatatcgt tgtggggctg ggcggatcgg ccaatggtgt tcccagggaa
     3241 accggttttg tcattacggc cgcttcggaa atcatggcca tcctggcact ttcaaaatct
     3301 cgtgctgatt taaagagaag gttgggcgaa atagtggtgg cgtacgacaa caaacaacaa
     3361 ttgatccgcg ccagggattt ggaggctcac agagccatgg cggttatcct gaacgatgcc
     3421 atcatgccta atctggtaca aaccaccgag aacacgcccg ccttggtgca tgccggtcct
     3481 tttgcaaaca ttgcgcacgg caccaacagc gttttggccg ataaaattgc acttaaactg
     3541 tctgattatg tggtgactga atgcggtttc ggaacggaat tgggcggcga gaagtttttc
     3601 aacatcgtct gccgcgacaa ccctatctgg ccctcggtcg ttgttattgt agccacctgc
     3661 agggcaataa aattccacgg tggcgttccc caaaagcctg catcggccct tcaggaagaa
     3721 aatttggaag catttgccaa gggtctgaaa aatttagagg tacacatccg aaacatgaaa
     3781 aagtttggtg tgcccgtgtt ggtagctatc aacaggttcc cgttcgacac agccaaggaa
     3841 attgagatga tcgaaaagct ttgtgccgaa cttaaagttg aatctgcccc ccatgaagcg
     3901 tttgccaaag gaggagcagg aaccatcgag ctggcagaga aaaccattca tatagtggaa
     3961 caatataaaa atccacaaaa gaaatttctg tacagtgtcg atatgcccat cgaagaaaaa
     4021 atccgtacga tcgctacaca gatgtatggt gcaaaagaca ttattatcag tccaaaaatc
     4081 agaaagaaat tagatcgatt tcttgaattc ggttttggca atttacctgt ttgtattgcc
     4141 aaaactcaaa tgtctttgtc agacgatccc aatatgattg gagcacccaa gggttgggtg
     4201 ctgactgttt ccgacgtcaa tctttcggca ggtgcgggct ttttggtagc tgtttgcggc
     4261 aacatgatgt tgatgccggg attgcccaaa gttcctgcgg cggtcaatat ggacgttgat
     4321 gacgatggtg aaataacagg attgttttaa agttgcttca tattctgata ttaccatctt
     4381 tgtagtctaa tgcccattag actgataaaa atagtttttt accggttaaa tagttgaacc
     4441 taatgaaaaa gaatttactt attatcattt tattgttgtt cacgtcaaca tggtcaatag
     4501 gacaggaggc cccggataag tcgggtccca aagagatagt caaaaccctg aaagcttcga
     4561 acctatcaga tatacaagcc agacaatttg ttgaaagccc tccgaccgga gagacttttt
     4621 acgaaggttt tgaagcagat gtatttcctc cggccggatg gaaggttatc aatgatggta
     4681 gttcagacca gacctgggtg agatatacgg aagaacctat aactggaaat gcgagtgcct
     4741 caattaggta ttcttcgcaa gcacacgatg actggctgat tacaccgcct ctggcgcctg
     4801 tagatggaaa taatgtcatt tcattcaggg caaaacagag aaccggtact tacatagaca
     4861 aattaatgta aaattatcca ccaccggcaa caacaaggaa gatttcgctg ttacgcttgc
     4921 ttcaaacctt ggaccggaat cgtcgacacc tgaaactttt acatacgatt taagcgcata
     4981 caacggtcag acggtctatt ttgctattca agcaatttcg accaatcagg aaaggctgta
     5041 tgtagatgat tttatcatgg c
//
LOCUS       DAOSGL010000154         5056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14740_length_5056_cov_3.610278, whole genome shotgun sequence.
ACCESSION   DAOSGL010000154 DAOSGL010000000
VERSION     DAOSGL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14740_length_5056_cov_3.610278"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKY68_05720"
     CDS             complement(<1..561)
                     /locus_tag="PKY68_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOH24569.1"
                     /translation="MDELIPFDLPSSEPAIIKVIGVGGGGGNAVTNMFRKGIRDVNYL
                     ICNTDKQAMLNSAIPDKLQLGPKTTKGLGAGNHPEIAKAAAEESMEDIKAAFGEHTRM
                     VFVTAGMGGGTGTGAAPVIAGIAREMDILTVGIVTIPFRWEGESKINQALDGVEEMSK
                     NVDALLVINNEQLFRIYPDLTVKNAFL"
     gene            complement(573..1946)
                     /gene="ftsA"
                     /locus_tag="PKY68_05725"
     CDS             complement(573..1946)
                     /gene="ftsA"
                     /locus_tag="PKY68_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOH24570.1"
                     /translation="MNNVQSYIAVLDPGSSNLILSLGYKDDMGKVNIVSSKSRPIDTE
                     IRRGVVQNVDQVGLMVEELMRELLRDLDRGCSIDRVYVGLNLYSTQCIDSSEKLNLNS
                     QELITEEILSELFDDAIARSLPENKEMIAHFEQEYLVDGFPCLNPKGERPDSLEGRYK
                     LIAARTQYMKNLENSLSNAGLKDCRNILGIQASAEAVLSEEDKNKGAVVIDFGGGTTS
                     MCIYKDNVLRYVCVIPYGGKNISRDLKQLNLSFSQAESLKLEEGTALHYTELMKKEAL
                     NKTEEEIRFFSEPLHDDKEINEIVVARSEEIVENIWAQIIYSGVEVGKIGSGIIICGG
                     ASKMDGLEDLLRIKTQMPVRKADLSCRLSSSCRVEFQNPAYAQTIGLIILGEKGGCRG
                     PLHSGQSKEQPRQATLPMEELEEITQKAPQKRPKTEAVKPDKKNKEKLISKIGNIFDD
                     LMKQGDY"
     gene            complement(1951..2685)
                     /locus_tag="PKY68_05730"
     CDS             complement(1951..2685)
                     /locus_tag="PKY68_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24571.1"
                     /translation="MAGRVFIWIGSVLMAGYVLFSVRLYSRQQNELQVQRVCIKVKDS
                     VDHPYVTSKEIGRLLKENQLQVIDKKLIEVDLSRIERVIDNHSMVKKAECFLSPSGCL
                     CIKIDQRIPVLRVYTGGGEAFFLDEEGKKMKINKRVPVDVPVALVQKTDSLSLAGLFE
                     LARLINKDDFWDAMIAQIVQESSGKLSLIPRLGELEIRLGKAENMEYKLRALRCFYEE
                     ALPRLGWDRYRCISTEFENQIVCTKK"
     gene            complement(2685..4061)
                     /gene="murC"
                     /locus_tag="PKY68_05735"
     CDS             complement(2685..4061)
                     /gene="murC"
                     /locus_tag="PKY68_05735"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOH24572.1"
                     /translation="MTIKQVYFIGIGGIGMSNLARYYHSRSCRVAGYDRTETELTKQL
                     VKEGMEIHYCDDVEAIPEAFKEVNTTLVVRTPAVSDMHSELQYFRSAGFRIVKRAEVL
                     GEISRELDALCVAGTHGKTTTSTLLAHLLRQSSLDCNAFLGGISLNYGTNLLLSGKSR
                     LMVAEADEYDRSFHRLSPWMAVITSVDPDHLDIYHSHEAYLEAFAHFTSLIRPGGNLL
                     MKQSLVLQPRLAEGVKTYTYATEQMPHSSKADFRAENITIEEGRLYFDFIYPGGVLKK
                     MELGLPITIHVENALAAMGLAYLCGVNAEELRPALASFKGNQRRFDFQIRRNDFVYID
                     DYAHHPNELKACIRSLKALYPTKELTGIFQPHLYSRTRDFADEFAQALSGLDYVYLLD
                     IYPAREEPIPGVDSKLIFDKMTIEGKTLCSMDEILSLLDRKKPEVLVTLGAGSIDRLV
                     PLLKEKYS"
     gene            complement(4058..>5056)
                     /gene="murG"
                     /locus_tag="PKY68_05740"
     CDS             complement(4058..>5056)
                     /gene="murG"
                     /locus_tag="PKY68_05740"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOH24573.1"
                     /translation="ILFVGAENRMEMEKVPAAGYPIIGLPVRGFDRQHLLSNVKTLFQ
                     LLRSMCKAHKLIREFKPDVAVGVGGYASGPLLRAAAAKKIPCLIQEQNSYAGITNKLL
                     AAGAARICVAYEGMERFFPKEKILLTGNPVRQDLLRSLNQKEAALKHFGLKPDRPVLL
                     VLGGSLGARSINRAVQQQLESIEKSGVQLIWQTGKYYYEEIMAANAELNNKAILITDF
                     ISRMDYAYALADLVVSRAGAGSISEFALLGKAVLLVPSPNVAEDHQTQNALALVRKGA
                     AVMISDEEACEKLIPEALALLEQPGRLQELRSAIKKMALPHAAEHIADEIIKLAKI"
BASE COUNT         1260 a         1302 c         1062 g         1432 t
ORIGIN      
        1 gagaaaggcg ttttttaccg tcaaatcggg gtagatgcga aaaagttgct cattgtttat
       61 caccaacaag gcgtccacgt ttttgctcat ttcctccacg ccgtccagag cctggtttat
      121 tttagattct ccttcccagc gaaaaggaat ggtaacaatt ccaacggtga gtatgtccat
      181 ttctcgggct atgccggcaa taacaggcgc tgctcccgtc cccgtgcctc cgcccattcc
      241 tgcggttaca aataccatgc gggtatgttc gccaaaagct gccttgatat cttccatact
      301 ttcttcggca gccgctttgg caatttcggg atgattaccg gcacccaaac ctttggtggt
      361 ttttgggccc aactggagtt tatcgggtat ggcagaattc agcatagctt gcttgtcggt
      421 attgcatatc aggtagttta cgtctcgaat tcctttacga aacatattgg taacagcatt
      481 accacctcct ccgcccactc ctatcacttt aataatggcc ggttcactgg agggtaaatc
      541 aaagggtatt aattcatcta tcatactcta tttcaataat ctccttgttt cattaagtca
      601 tcaaaaatat tgccgatctt gcttatcagc ttctctttat tctttttatc cggctttacc
      661 gcttctgttt tgggcctttt ttggggtgct ttttgcgtga tttcttccaa ttcttccatg
      721 ggtaaagttg cctgcctggg ttgttcttta ctctgcccgg aatgtaaggg gccacggcaa
      781 ccacctttct ccccgagtat gatcaaacct atggtttgtg cgtatgccgg attctgaaat
      841 tccacacgac aggaagagga taaacgacag gacaaatcgg ctttacgtac gggcatttgt
      901 gtttttatcc ggagtaaatc ttccaatccg tccatttttg atgctccccc gcatataata
      961 ataccggatc cgattttgcc gacttctact cctgaataaa taatttgagc ccaaatattt
     1021 tcaacaatct cttcgctgcg ggctaccaca atttcgttga tttctttatc gtcgtgtaaa
     1081 ggttcgctga aaaagcgtat ttcttcttcc gttttgttga gagcctcctt tttcataagc
     1141 tcggtataat gcagggctgt tccctcttct aatttcagcg actcggcctg ggaaaaactc
     1201 aggttaagtt gcttcaaatc gcggctgatg tttttacctc cataaggaat aacacaaaca
     1261 taacgaagta cattatcctt ataaatacac atcgaggtgg tacctcctcc aaaatcaatc
     1321 acgacagctc ccttattctt gtcttcttcg ctcaggactg cttcggcgct ggcctgtata
     1381 cccagaatat ttcggcaatc tttcaaaccc gcgttgctca ggctattttc aaggtttttc
     1441 atgtactggg tcctggctgc aatcagctta tatcggcctt cgagcgaatc gggacgctct
     1501 cctttcggat tgaggcaggg aaaaccatcc accaggtatt cttgttcgaa gtgggcaatc
     1561 atctccttgt tttcgggcag agatctggct atggcgtcat cgaaaagctc cgaaagtatt
     1621 tcttcggtaa tcagttcctg tgaattgagg ttgagttttt cactactatc gatgcattgc
     1681 gtactgtaaa gattcaatcc cacatacacc cggtcaatgc tgcaaccgcg gtccagatct
     1741 ctgagtaatt cccgcatcaa ttcttctacc atcaggccta cctgatccac attctgcact
     1801 acaccccgac gaatctcagt atcaataggc ctggacttcg aagacacaat gttcactttc
     1861 cccatatcgt ccttataccc caggctaagt atcaaattgg agcttccggg gtccagtacg
     1921 gctatgtacg attgaacgtt attcatatgt ttattttttt gtacaaacta tctgattctc
     1981 aaattcagtg ctgatgcaac ggtatctatc ccaacccaac ctgggtaaag cttcttcgta
     2041 aaagcaacgc aacgccctta gcttatattc catattttct gctttgccca acctgatctc
     2101 aagctctccc aatctgggaa taaggctgag tttacccgaa gattcctgca caatctgtgc
     2161 aatcatggca tcccaaaaat cgtctttgtt tatcaggcga gccaattcaa acaaaccggc
     2221 cagactcaaa gaatctgttt tctgcaccaa agctaccggc acatccaccg gtactctttt
     2281 gttgattttc atctttttgc cctcttcgtc cagaaaaaac gcttctccac ctccggtata
     2341 caccctcagg acgggaatac gctgatcaat cttaatacac aagcagccgg aaggcgaaag
     2401 aaagcattcg gcttttttta ccatagaatg attatctatc accctttcta tgcgactcag
     2461 gtctacctct attaacttct tgtcaattac ctgcagttga ttttctttca acaggcggcc
     2521 aatttctttt gatgtaacat aaggatggtc cacactgtct ttcactttga tacaaaccct
     2581 ttgaacctgc agctcgtttt gttgcctgct gtagagcctc acggaaaaca ggacatagcc
     2641 tgccatcaac acggagccta tccatataaa cacccttccg gccatcagga atacttctct
     2701 tttaataatg gcaccaaacg gtcaatactt ccggccccca aagttacaag aacttccggt
     2761 tttttcctat ccagcagact taagatttcg tccatggaac aaagggtttt cccttcgatg
     2821 gtcattttgt caaatataag tttggaatcc actcccggaa taggctcctc cctggccgga
     2881 taaatatcga gcaggtaaac ataatccaaa cccgacaggg cttgagcaaa ttcgtcggca
     2941 aagtcacgcg tccggctgta aagatggggc tgaaaaatac cggtcagttc tttagtagga
     3001 tatagtgctt tgagcgagcg gatacaggct ttgagttcat tgggatgatg cgcataatcg
     3061 tcgatataaa caaaatcgtt gcggcgaatc tgaaaatcga aacgacgttg attgcctttg
     3121 aacgaagcca aagccggtcg caattcttct gcattcactc cgcagaggta ggccaggccc
     3181 atggccgcca gagcgttctc cacatgtatc gtaatcggca ggcctaattc catttttttc
     3241 aacacacccc ccggataaat gaaatcaaaa tacagacgcc cttcttctat cgtaatgttt
     3301 tccgcccgaa aatcggcctt gctgctatga ggcatttgct ccgtagcgta agtgtacgtt
     3361 tttacaccct cggccaaacg aggctgcaaa accagactct gtttcataag caagttcccg
     3421 cccggacgga tcaaagaagt aaaatgggca aaagcttcca ggtaagcttc gtggctgtgg
     3481 taaatatcca gatgatcggg atctaccgaa gtaatcacag ccatccaggg gctgagccgg
     3541 tgaaaagacc tgtcgtattc atcggcttct gccaccataa gccggctttt accggagagc
     3601 aaaaggttgg taccgtagtt gagggatatt ccgcccaaaa aggcattgca atcgagcgaa
     3661 gattgtctga gcaaatgggc cagcaacgta ctggtggtgg ttttgccgtg agttccggcc
     3721 acacataagg catcgagttc tcgtgaaatc tcacccagca cttcggcacg cttcacgatt
     3781 ctgaagccgg cagaacggaa atactgcaat tcggaatgca tatcggaaac agcaggtgtt
     3841 ctcactacca aggtggtatt cacctctttg aaagcttcgg gaatggcctc cacgtcgtcg
     3901 cagtaatgta tttccatgcc ttcttttacc agttgtttgg tcaattcggt ttccgttctg
     3961 tcgtatccgg ccacacggca agaacgggaa tggtaataac gggcaagatt gctcatgcct
     4021 ataccgccaa tcccgataaa gtatacctgc ttgattgtca tatcttcgct aattttatta
     4081 tttcatctgc tatgtgttcg gcagcatgtg gcaaagccat ttttttgatg gcgcttctca
     4141 gttcttgcaa gcgtccgggc tgttccagca aggccaaagc ctcgggaatg agtttttcgc
     4201 aagcttcctc atcgctgatc ataacggcgg ctcccttgcg caccaaggcc agggcatttt
     4261 gcgtctgatg atcttccgcc acattgggcg aaggaaccag caaaaccgcc ttacccaaca
     4321 gggcaaactc cgaaatggaa ccggcacccg ccctcgacac gaccaaatcg gccagggcat
     4381 acgcatagtc catcctcgaa ataaagtccg tgattaggat agccttattg ttgagttctg
     4441 cattagcagc cataatttct tcgtaataat acttgccggt ttgccagatc aattgcactc
     4501 ccgatttttc tatggattca agttgttgct gcactgcccg gttgatacta cgagcgccca
     4561 ggctgcctcc caggacaagc aaaacagggc gatccggctt tagcccgaaa tgcttcaggg
     4621 ccgcttcctt ttgattgagt gaacgaagta aatcctgacg cacgggattg ccggtaagca
     4681 aaatcttttc tttcgggaaa aagcgctcca tgccctcata agccacacaa atacgggcag
     4741 cccctgctgc aagtaattta ttggtaattc cggcatacga attttgttcc tgtatgagac
     4801 aaggtatttt tttggcagcc gcagccctga gcaaaggacc gctggcataa ccgcccactc
     4861 cgaccgctac gtcgggctta aattcgcgta tcaatttgtg ggctttacac atacttctca
     4921 gcaactgaaa caaggttttg acattactca gcaaatgttg acgatcaaaa cctctcacag
     4981 ggagtccgat gataggatag cctgctgccg gcactttttc catttccatg cggttttcgg
     5041 cgccgacaaa aagtat
//
LOCUS       DAOSGL010000155         5043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14786_length_5043_cov_3.879110, whole genome shotgun sequence.
ACCESSION   DAOSGL010000155 DAOSGL010000000
VERSION     DAOSGL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14786_length_5043_cov_3.879110"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..2455)
                     /locus_tag="PKY68_05745"
     CDS             complement(152..2455)
                     /locus_tag="PKY68_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH24574.1"
                     /translation="MSFKRSILIAFCLSLVFYAQAQRVGLVLSGGGAKGLAHVGLIQA
                     LEENQIPIDYVTGTSIGAIVGSLYAMGFSPDEMMALFLSEDFTYWSTGTIQTKDIDYF
                     RKGDLSPEFFSTYLSLSDSLLSNPRSLVPGSLINASQLNYAFVQVYSQYTALCGGDFD
                     RLFVPFRCVATDVHSKQAVIFKNGRLEDAVRASMSFPLVFNAVSVDSMLLLDGGIYDN
                     YPVKLMQKEFNPDFVIGSNVSIVNSKPSEDDVVSLIEHIIIQPTHFEASPDEFIEFKF
                     DLKDVYLMDFHKALETYQKGYDLGISMIDSIKGIISRRQDPDSLAQKRQAFKANLPEL
                     IFDELELTGLNHQQEEFVRRMIGYDKDRRFSLKEFRKAYYAMIADTRISQIIPKVWYN
                     PSTGLFKLKLDIKTEKNLVFALGGNISSSTSNLLYARIGYQRFGKVATYYSADTYIGS
                     IHNALRLNTRWDVAGYIPSYLQASLSVSSFKFYEGEKLFYEELSPAFIQEREILFKLR
                     YGIPAFSQAKFEIGYSVGRISDWYMQSYLSNITRANFDRSYYDLSNFSLRLEKNNLNY
                     RQYPTGGSHYYLLCQYLLGEENYSYPDSVGNRFKESKELGYFQASAAYEGYLNFNKSI
                     TLGCILNAVYNNKRSLDNYTASIIQAPAFTPTPYTLSTFNEAFRSNKYAAIGIMPIWT
                     IRPFLHLRSEFYTFMPLDAIKRGPNQETVLSRSFSNTQFLGELSLVFHQPFVSMSLFT
                     NYLSYPKNNWNVGINIGFLIFNKRLVE"
     gene            complement(2511..4364)
                     /gene="htpG"
                     /locus_tag="PKY68_05750"
     CDS             complement(2511..4364)
                     /gene="htpG"
                     /locus_tag="PKY68_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOH24575.1"
                     /translation="MQKGRIGVTTDNIFPVIKKFLYSDQEIFLREVISNAVDATQKLK
                     TLASVGEFKEELGDLSIRVSLNSKNKTLTVSDRGIGMTAEEIDKYINQIAFSGAEEFL
                     EKYKTDANAIIGHFGLGFYSTFMVSSRVEIVTKSWKEGSKAVRWSCDGSPEYELEEVE
                     KDDRGTDIILYIDNKDYLKEEKINELLNKYCKFLPIPIIFGKKKEWKDGKYQETDQDN
                     QINETEPTWTKKPGKLKEEDYLKFYKDLYPFSEDPLFHIHLNVDYPFHLTGILYFPKI
                     KNSIEIQRNKIQLYCNQVYVTDSVEGIVPNFLTLLHGVIDSPDIPLNVSRSYLQHDDN
                     VKKISNHITKKVGDRLKKIFKDDRKQFEEKWDDLKIFIQYGILTEEKFWDYAKEFSLM
                     QNIDGKYFTYEEYQTLIKEEQTDKDGQLIYLYASDKESQYAYIETAGNAGYDVLLMNG
                     ELDVPLMQHLEQKFEKSRFVRVDSDVIENLIQKKDREQLNLSEEEKESLKKLFSEQLP
                     AMDKVRFELQLDALGENSLPIVLTQNEFMRRMQDMSGLQPGLSFYADMPVSYSMVLNT
                     EHKLIKELIGKELDEKNKNTVNQLIDLALLSNGMLKGESLIKFIKRSCELI"
     gene            complement(4496..4984)
                     /locus_tag="PKY68_05755"
                     /pseudo
     CDS             complement(4496..4984)
                     /locus_tag="PKY68_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         1430 a         1011 c         1010 g         1592 t
ORIGIN      
        1 tagagcatct gactacggat cagaaggtta caggtttgaa tcctgtcggg gtcaccacat
       61 acccgaaaag aacaaggcta caaggcgtaa aatcagccag gtagcttttt taatttttcc
      121 ccatcacttt gccaataatc ctaaaaatcc atcattcgac aagacgttta ttgaagatta
      181 ggaaaccaat gttgatgcca acgttccaat tatttttggg atagctcagg tagttggtaa
      241 acagactcat ggacacaaaa ggctggtgaa acaccagaga taactcacca aggaattgag
      301 tattcgaaaa agatctgctc agcactgttt cttgattagg tccccgtttg atagcatcta
      361 gaggcataaa ggtgtaaaat tcagatctca gatgcaaaaa aggacgtatg gtccaaatag
      421 gcataattcc tatggcagcg tatttgttag aacgaaaagc ttcattaaaa gtactcaagg
      481 tgtagggtgt gggagtaaaa gccggtgcct gtatgatact agccgtatag ttatccaaag
      541 aacgcttgtt attgtaaaca gcatttagta tacagcccag agtaatactt ttattaaaat
      601 ttaaatagcc ttcataggca gccgaagcct ggaaataacc caattctttg ctttccttga
      661 agcggttccc cacagagtcg ggataagagt agttttcttc ccccagcaaa tattgacaca
      721 aaagatagta atggctgcct cccgtcgggt attgcctata atttaaattg tttttttcca
      781 aacgaaggga aaaattcgac aagtcgtagt aactgcggtc aaaattggct cgagtgatat
      841 tgcttaagta cgattgcata taccaatcgc tgattcttcc cactgaatag ccaatttcaa
      901 atttagcctg actaaatgca ggtataccgt aacgaagttt gaaaagaatt tctctttcct
      961 gtataaaagc cgggcttaat tcttcgtaaa ataatttttc accttcgtag aacttgaagc
     1021 tgcttaccga aagactggct tgcaaataac tgggaatata ccccgccaca tcccatcgcg
     1081 tgttcaatct caaagcgttg tggatactgc ctatgtatgt gtcggctgag taataggtgg
     1141 ccactttacc aaatcgttgg taacctatac gtgcatacaa aaggttggag gtagaagaag
     1201 agatgtttcc gcccaaagcg aataccaggt tcttttcggt ttttatatcc agtttcagtt
     1261 taaacaatcc ggtggaagga ttgtaccaga ctttgggaat gatctgagat attctggtat
     1321 cggcgatcat ggcataatag gccttgcgga actctttgag cgagaacctg cgatctttgt
     1381 cgtagccgat cattctgcgt acaaattctt cctgttgatg gttgagaccg gtgagttcga
     1441 gttcgtcgaa aataagctca ggaaggtttg ctttgaaagc ctgtcttttt tgagccaatg
     1501 aatccggatc ttgccttctg gagattattc ccttaatgga gtctatcata gaaatcccta
     1561 aatcgtagcc tttttgatag gtttccaagg ctttgtgaaa atccatcagg tacacgtcct
     1621 tcaagtcaaa tttgaattct ataaactcat cgggagaagc ttcaaaatgt gtgggttgaa
     1681 ttattatatg ttcaattagc gaaaccacat cgtcttcgct gggcttgctg ttaactatag
     1741 aaacgttgct acctatgaca aaatcggggt tgaattcttt ttgcatgagc ttcaccggat
     1801 aattatcgta gattcccccg tcgagcaaaa gcatactgtc cacactaacg gcattgaaaa
     1861 ccaaagggaa actcatggaa gcgcgtacgg catcttccag tctgccattc ttaaaaatta
     1921 cggcttgttt gctgtgtaca tcggttgcaa cgcaacgaaa agggacaaac aatcgatcga
     1981 aatcacctcc acacaaggct gtatactgac tataaacctg tacaaaagcg tagttgagct
     2041 ggctggcatt aatcaggctg ccgggtacta aagatctggg attggacaaa aggctgtcgc
     2101 taagagaaag ataggtagaa aagaattccg ggcttaaatc tccttttcta aaatagtcga
     2161 tgtctttagt ttgaatggtt ccggttgacc agtaagtaaa gtcttccgac aaaaacaggg
     2221 ccatcatttc gtcaggagaa aagcccatgg cgtacaagct gcccacaatg gcaccaattg
     2281 aggtccctgt gacataatcg atgggaattt ggttctcttc cagggcttgt atcagcccca
     2341 cgtgtgccag cccttttgct ccgccgccgc ttaatacaag accaacgcgc tgagcctggg
     2401 cataaaatac caggctcaga caaaaggcaa taagtatgct gcgcttaaag ctcatttatt
     2461 atcgcagatt tggtaacaca aagttatcaa aagtacgata attatcccgg ttaaatgagc
     2521 tcacaactcc ttttaataaa ttttatcagt gattcgccct tgagcatgcc attactcagg
     2581 agagcgaggt caatcaattg atttacagta tttttattct tttcgtcgag ttcttttcca
     2641 atgagttcct tgatcaattt atgctctgta ttcaaaacca tgctgtaact tacaggcatg
     2701 tctgcgtaaa aactcaaacc gggttgcagg ccggacatat cttgcatacg acgcataaat
     2761 tcgttctggg tgagcacaat aggcagactg ttttcgccaa gagcgtccaa ttgcaattcg
     2821 aaacgaactt tgtccattgc aggtaattgt tcgctaaaca actttttcag actttctttt
     2881 tcctcttcac tcagattaag ctgttctctg tccttttttt ggatcaaatt ttcgatgaca
     2941 tcgctgtcta cacgaacaaa acgcgacttt tcgaattttt gttccaggtg ttgcatcaga
     3001 ggtacgtcca attccccatt catcaataag acatcgtaac cggcattccc ggctgtttca
     3061 atataggcgt attgactttc tttgtcgctg gcgtacagat aaataagctg accgtccttg
     3121 tctgtttgtt cttctttgat cagggtctgg tattcttcgt atgtaaaata tttgccgtca
     3181 atattttgca tcagactgaa ttccttggca tagtcccaga atttttcttc cgtcaaaata
     3241 ccgtattgaa taaatatctt aaggtcgtcc catttttcct caaattgttt acgatcatct
     3301 ttgaaaatct ttttcaggcg atcgcctact tttttggtga tatgattgga tatctttttt
     3361 acgttgtcgt cgtgttggag gtaagaacgg gatacattca atggaatatc gggcgaatcg
     3421 ataactccgt gcagtaaagt caggaagttt ggcacaatgc cttctacgga atcggtcaca
     3481 taaacctggt tgcagtacaa ctgaattttg ttcctttgaa tttctatact gtttttaatt
     3541 ttggggaaat acaatatacc ggtcagatga aaaggataat ccacgttcag gtgaatatgg
     3601 aatagcggat cttcgctgaa agggtagagg tctttgtaaa atttcaaata atcttcttct
     3661 ttcagtttgc cgggtttctt ggtccaggtc ggttcagttt cgtttatttg gttatcctga
     3721 tcagtttcct ggtattttcc atctttccat tctttctttt tgccaaagat gatgggtatg
     3781 ggcaggaact tacagtattt attgagcagt tcgtttattt tttcttcttt caggtaatct
     3841 ttattatcga tatacaggat aatatcggtg ccacggtcgt ctttttcgac ttcttccagt
     3901 tcatactcag gcgatccgtc gcaactccag cgcacagctt ttgatccctc tttccaggat
     3961 ttggttacaa tttcaacccg gctcgaaacc ataaaagtag aataaaaacc taaaccgaag
     4021 tggccaataa tggcattagc atcggtctta tatttttcga gaaattcttc agctcctgaa
     4081 aaggctattt ggttgatgta tttatcgatt tcttccgcag tcataccaat tccacggtcg
     4141 gaaacggtca aggttttgtt tttgctgttt aagcttacgc gaatggataa atccccgagt
     4201 tcttctttaa attctcccac cgaagccagt gttttgagtt tctgagtggc atcgacggca
     4261 tttgaaatga cctcacgtaa aaaaatttcc tgatcgctgt ataaaaactt ttttatgacg
     4321 gggaagatat tgtctgtagt aaccccaata cgtccttttt gcataatttg atatatttaa
     4381 aaatacgata aaatgaatga ataaagctgt ttttctatct catttggaac aaaaaaaatg
     4441 ccaaacacca gggaatgaca ttttttgatt ttgactgcta ttttggcagc tatttttatt
     4501 tttcaagctt attcgagaga ataatcttgc tttcagcact atccaattcg actaaaatat
     4561 gcttgtttgt tttgaatttc tcactcaaaa tcacttcagc cagttcgtct tcgaggtatt
     4621 tttgaatggc tcttttcaga gggcgggcac caaattgcac atcataacct tgttcggcaa
     4681 tgaaattctt ggcttcgggt gtaatgtcta gtgtataacc taagctttcc acccgctgat
     4741 agaaggcttt caattccaga tcaataattt tcaagataga atctttggac aattgctcaa
     4801 aataaatcgt atcgtcgata cgattcaaaa attcaggagc aaatgttttg gttattgctt
     4861 tatgaatcaa agatttagaa taatcattct gtttgatgga atccggatag ccaaagccta
     4921 cgcctttacc gaattccttg agttgacgag tgcccacgtt ggaagtcata attaaaatgg
     4981 tgttttatta aaattgattt ctaaattttt aaaaccaaac ggtaaattta tttttgtaga
     5041 att
//
LOCUS       DAOSGL010000156         5037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_14822_length_5037_cov_3.666399, whole genome shotgun sequence.
ACCESSION   DAOSGL010000156 DAOSGL010000000
VERSION     DAOSGL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14822_length_5037_cov_3.666399"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="PKY68_05760"
     CDS             complement(<1..1960)
                     /locus_tag="PKY68_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH24576.1"
                     /translation="MKRVFLCLAAGIFVVHSMAAAKTDSIRQLDLNDVVVVASYAGEN
                     TPVTHQNLSKAEIRTLNVVSTLPQVLWMTPSLVHTAENGTSTGNTAMRIRGTDASRIN
                     FSLNGIPMNNPESQEVYWVNIPNLTGSLQSIQIQRGVGVSANGTGAFGASVNLLSERP
                     AADNYIESSTTAGSYGTFEQSVSLATSRLPGGVQMQLRYTALNADGYLRNGWSRHQSL
                     YGTLGKSWEKAQLSMNYIYGNQHTGITWEGASKEQIALNPAYNPAGAIKDGLYYGNES
                     DNYRQHHIQLFYSRELTETLLLNTGLNYTDGYGYYEQYKQNRKFSSLGLSNQLIDGIE
                     YDRSDHIRGKFMANGFYTGLFNLNYQPGAFRLQGGGMYSFYQGVHYARLLWLEHNNNI
                     PANYEWVRNFGEKTDGNLFIKAEYALRNKLFLYADLQYRNVRYELEGIDDDDLLDLSQ
                     LHKWQFWNPKMGVNWRIDQSRKLYLSAGIANREPTRADLKDAVKGGSRRVLPERLYDV
                     ELGYSVNKPKWMLALNLYDMEYHNQLIPTGKLNEVGYKLMSNVPYSYRRGMELSADIR
                     PFSVLQLDANLSLSTNKVKDFSLYYSTYDNASDWNEILDQSRQISSYYSMADLPFSPA
                     CIAAARLQYQPVPSLQLSLMGKYIAEQQF"
     gene            2245..3438
                     /locus_tag="PKY68_05765"
     CDS             2245..3438
                     /locus_tag="PKY68_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOH24577.1"
                     /translation="MGKVLIIGAGGVGSVVAYKVAQHPEVFSEIVLASRTKAKCDKIA
                     AFVGNKRIQTDQVDADSVEQLVALFKKHRPELVIHVALPYQDLTIMDACLACGVHYLD
                     TANYEPKDKAHFEYSWQWAYKERFEKAGLTAILGCGFDPGVTGVWTAYAAKHHFDEIH
                     YLDIVDCNAGDHGKAFATNFNPEINIREVTQKGRYYENGQWIETEPHQIHKTLNYPEI
                     GPRESYLIYHEELESLVKNFPTIKRARFWMTFGEEYLTHLRVIQNIGMAGIEPVMFKG
                     MEIIPIEFLKAVLPDPGELGENYTGQTSIGCRIRGIKNGKERTYYVYQNCSHQAAYKE
                     TGAQAVSYTTGVPAMCGAMMFMKGLWRKPGVFNVEEFDPDPFLKALAENGLPWHELFD
                     VDLEL"
     gene            complement(3468..3965)
                     /locus_tag="PKY68_05770"
     CDS             complement(3468..3965)
                     /locus_tag="PKY68_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOH24578.1"
                     /translation="MKKIAILSDTHDYWDERYIPYLEICDEIWHAGDIGSVLLTQRLE
                     AIRPLRAVYGNCDGYPLRYNFSPHLFFELEQVRVLMTHIGGYPGRYDSSALALIKHHK
                     PNLFVCGHSHIAKVMFDRRHDMLCVNPGAAGKQGFHTIRTLMRLELNEGNIENLEVIE
                     LGKIV"
     gene            complement(3968..>5037)
                     /locus_tag="PKY68_05775"
     CDS             complement(3968..>5037)
                     /locus_tag="PKY68_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HOH24579.1"
                     /translation="TFDLILADMPCSGEGMFRKEPEALAAWSEDNVLGCALRQRNILA
                     QCWNSLRPGGCLIYSTCTYNTEENEKNVLWAAENLGARVLPVECNPDWNIHPAIEADI
                     PAYRFFPHRTRGEGFFLALLQKEEALAANSGTGAANSLTARANSGTDAVNSLTGVADS
                     QILAANPQTGLENYQQKNFKEPFRPKKGRNKSPSGKKFGKFVNEVMDKKKLSHLNSLF
                     DEPEAYEISLLPWGVYRAFPTQHAAFLARISNSLRIVRAGLYLGEYKGNQFIPDVSLA
                     LSTALKAGKHFPSYELHRQQALAYLKRESLQLSEDCPRGQVLVTYGRVGLGWVNNLGI
                     RANNRYPASWKIRSQLPVNQF"
BASE COUNT         1327 a         1194 c         1166 g         1350 t
ORIGIN      
        1 taaactgctg ctccgctata tattttccca ttaagctcaa ttgtaaagag ggcacgggct
       61 gatactgaag acgggcagca gcaatacatg cgggagaaaa aggcaaatcg gccatggaat
      121 aatagctgct gatctgccgg ctttggtcaa gtatctcatt ccagtccgag gcattatcgt
      181 aggtactgta atacaggctg aaatctttaa ccttattggt actaagactc agattggcat
      241 ccaactgcaa gacggaaaag ggcctgatgt cggcactcag ttccattcct cggcggtaac
      301 tgtagggcac attgctcata agtttataac ccacctcgtt tagtttgcct gtaggtatca
      361 actggttgtg gtattccata tcgtacagat tcagggctag catccatttt ggtttgttga
      421 ccgaataacc cagctcaaca tcgtacagtc tttcgggtaa gacccgtcgg ctgcctcctt
      481 taacggcatc cttcaagtct gcacgggtgg gttcccgatt ggcaatacct gccgaaagat
      541 atagtttccg actctggtct atgcgccagt ttacacccat tttgggattc cagaactgcc
      601 atttatgtaa ttggcttaaa tccagcaaat catcgtcatc aattccctcc agttcgtatc
      661 tcacattacg gtattgcagg tcggcataca aaaaaagttt attccgaaga gcatattcgg
      721 ccttaataaa taaattgccg tctgtttttt cgccaaaatt acgcacccac tcgtagttgg
      781 cagggatatt gttgttgtgt tccagccata ataaacgggc gtagtgaacc ccttggtaaa
      841 aagagtacat gcctccgccc tggagcctga aagctcccgg ttggtagttc aaattgaaca
      901 atccggtata aaaaccgttg gccatgaact ttccccggat atgatcgctg cggtcgtact
      961 ctattccgtc gatcaattgg ttgcttaaac caagggacga aaactttctg ttttgtttat
     1021 actgttcata gtagccgtac ccgtccgtgt aattcaatcc ggtattcaac aaaagcgttt
     1081 ccgtaagttc ccgggaataa aagagctgaa tatggtgctg gcgataattg tcgctttcgt
     1141 tgccgtaata caagccgtcc ttgatggctc ctgccggatt ataggccgga ttcaaagcaa
     1201 tttgttcttt ggaagctcct tcccaggtta tacctgtgtg ctgattgccg tatatgtaat
     1261 tcatgctcag ctgagctttt tcccaggact tgcccagcgt gccgtagagc gattgatgac
     1321 gactccagcc gttgcgcaaa taaccgtcgg cattcaacgc tgtatagcgt aattgcatct
     1381 gcacccctcc gggcaggcgt gaagttgcca aagaaacact ctgttcaaaa gtaccgtaac
     1441 tacctgccgt tgtggaagat tctatataat tatccgctgc cgggcgctcg ctcagtaaat
     1501 tgacagaagc tccaaaggct ccggtaccat tggccgacac acccacccca cgctgaattt
     1561 gtatgctttg tagcgatccg gtcaggttgg gaatattcac ccaatacact tcctgcgatt
     1621 ccgggttgtt catgggtatc ccgttcaaac tgaaatttat gcggctggcg tccgttcccc
     1681 tgattcgcat ggccgtgttg ccggtgcttg tcccgttttc ggcggtatgc accagcgagg
     1741 gagtcatcca aagcacctgg ggcaatgttg aaactacgtt cagagtgcga atctcggctt
     1801 tgctcaggtt ttgatgagtt accggggtgt tttcccccgc ataggaggca actaccacca
     1861 catcgtttaa atcaagttgt ctgatactgt ctgtttttgc ggctgccatg gaatgaacga
     1921 caaaaatgcc cgccgcaagg cataaaaaaa cccttttcat gtttttcttc ttaatagtga
     1981 aacatcaaaa ggggtgtaag agaaaacatt tcccgacggt agcattacct acatccggtc
     2041 ttgggtataa tctcagatca ctcaaagcgt agtagtttta aagtgtcgtt ttgtccgact
     2101 taaaataaaa cgacaaaaag aactgtatgc cgagggaaca cccctaaaaa atccacgcaa
     2161 atgtaagcaa tatttttgtc tgcccctatt tcggggcata ttttaaaaat aattatcttt
     2221 gtagacttat taaacacaaa gattatggga aaagttctta tcatcggagc cggcggagtc
     2281 ggaagcgtag tagcatacaa agtagcacaa catccggaag tattcagcga aatcgtgttg
     2341 gccagccgta ccaaggcaaa atgcgataag attgctgcct ttgtgggcaa caaacgtatt
     2401 cagaccgatc aggtggatgc cgattctgta gagcagcttg tggcattatt caaaaaacac
     2461 cgaccggagt tggtcattca tgttgccctg ccctatcagg atttaaccat catggatgcc
     2521 tgccttgcct gtggtgttca ttacctcgac acagccaatt atgaaccaaa agacaaggcg
     2581 cattttgagt attcctggca atgggcctac aaggaacgtt tcgaaaaagc cggtttaaca
     2641 gccattctgg gttgcggttt cgatcccggt gtaaccggtg tgtggactgc ttatgccgcc
     2701 aaacatcatt ttgacgaaat tcattacctg gatatagtgg attgcaacgc cggcgatcat
     2761 ggtaaagctt ttgcaaccaa tttcaatccg gagatcaata ttcgtgaggt cacacaaaaa
     2821 ggcaggtatt acgaaaacgg ccaatggatt gaaaccgaac ctcatcaaat acataaaacc
     2881 cttaattatc cggaaatagg acccagagaa tcttacctga tttatcacga agaacttgaa
     2941 tcgctggtta aaaatttccc gaccatcaaa cgggctcgtt tctggatgac ctttggagaa
     3001 gaatacctta cccatttgcg tgtaatccaa aacatcggaa tggccggcat tgaacctgtt
     3061 atgtttaagg gaatggaaat tatccccatt gagtttctga aagctgtcct gcccgacccc
     3121 ggcgaactgg gagaaaatta caccggccag acatccatag gctgccgtat ccggggcatc
     3181 aaaaacggca aggagcgaac ttattatgtt tatcaaaatt gcagtcacca ggccgcttac
     3241 aaagaaaccg gcgctcaggc tgtaagctat actaccggcg taccggccat gtgcggagcc
     3301 atgatgttta tgaaaggttt gtggcgtaaa cccggtgttt tcaatgtgga agaattcgat
     3361 ccggatcctt ttctgaaggc tttggccgaa aacggtttac cctggcatga acttttcgat
     3421 gtagatctgg agctctaaac catcgtattc aaggcatata gcttcagtta tacaattttg
     3481 cctaattcta ttacttcgag gttttcgata ttaccctcgt tcaattcgag acgcataagc
     3541 gtacgaatgg tgtgaaaacc ctgtttgccg gcggctcccg gattgacaca aagcatatcg
     3601 tgtcgacggt caaacatcac tttggctata tgggaatgtc cgcaaacaaa caaattcggt
     3661 ttgtgatgtt tgatcagagc cagtgctgag gaatcgtaac gccccggata accgccaata
     3721 tgcgtcatca acaccctgac ctgctccaat tcaaaaaaca gatggggact gaaattatac
     3781 cttaagggat aaccgtcgca attgccatac acggctctta gcggcctaat agcttcgagg
     3841 cgttgggtca gcaaaacaga accaatgtcg ccggcatgcc agatttcgtc gcaaatctcc
     3901 aggtaaggaa tgtagcgttc atcccaatag tcgtgagtgt ccgataaaat agcaattttc
     3961 ttcatgatta gaattgatta acagggagtt gagagcgtat cttccaggaa gcgggataac
     4021 ggttattcgc ccttattccc aaattgttta cccaacccag gccgactcgg ccgtaagtca
     4081 ccagcacctg accccgcggg caatcctcac ttaactgcaa actctctctt ttcaaataag
     4141 ccagagcttg ttgacgatgc agttcatacg aagggaaatg tttgccggcc ttaagggcgg
     4201 tagaaagagc cagtgaaaca tcgggtataa actgattgcc tttatactct cccaggtaca
     4261 gaccggcacg tactatgcgc agggaattac taatccgggc cagaaaagca gcgtgttgtg
     4321 tcggaaatgc cctgtaaaca ccccatggca gaagactgat ttcgtaggct tcgggctcgt
     4381 caaacagaga gttcaaatgg ctaagtttct ttttgtccat aacttcgttc acaaattttc
     4441 cgaatttttt cccggagggt gatttgtttc gacccttctt tggtctaaag ggctccttga
     4501 agtttttttg ttgataattt tccaaaccgg tctgtgggtt tgctgcaaga atctgtgagt
     4561 ctgctacgcc ggtcagtgag tttacggcgt cggtccctga gtttgcccga gcagtcagtg
     4621 agtttgctgc gccggtccct gagtttgctg caagagcctc ctctttttga agcaaagcta
     4681 aaaagaaacc ttctcctctg gttctatgtg ggaaaaaacg ataagccgga atgtcggctt
     4741 cgatggcagg atgaatattc cagtcgggat tgcattctac aggcagaacg cgggcaccta
     4801 gattttcggc tgcccacaat acgtttttct cgttttcctc tgtattgtag gtacatgtac
     4861 tataaatcag gcagccgcct ggccgcagac tgttccagca ttgagccagg atattacgtt
     4921 gacggagcgc acagccgagg acattgtctt cggaccaggc agccaaagcc tccggttctt
     4981 tgcgaaacat accctcgccc gagcagggca tatcggccag gatcaaatca aaagtat
//
LOCUS       DAOSGL010000157         4954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15143_length_4954_cov_3.495203, whole genome shotgun sequence.
ACCESSION   DAOSGL010000157 DAOSGL010000000
VERSION     DAOSGL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15143_length_4954_cov_3.495203"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1396)
                     /locus_tag="PKY68_05780"
     CDS             complement(224..1396)
                     /locus_tag="PKY68_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH24580.1"
                     /translation="MKKISKLLLLLPLLAFFVACEYGIDITPAGKHSAEYADGTGSPG
                     NNQGSGGAQAGVVTAAEWNDLDNWTFWTDLINDTIYNTYPPYWKFYPKHRVAIRINHG
                     ANPVINAALELKKAEYSVWKARTDNLGRAEMWISLFQKTERISMSDYVLYIDGKAWTG
                     ELKSFDQGWNEISLSSAGENSNRVELAFVVDATGSMADELEFLKSDLQDVLDSVSKIN
                     SSLNLFTGAVFYRDEGDEYVVKHSGFTAETATTIDYIGAQRADAGGDYPEAVHTALNT
                     TLTELQWSEQARTRIAFLLLDAPPHYTTAVISDIHASLQLAAAKGIKIIPITASGIDK
                     ETEFLMRLMAMTTNGTYVFITNDSGVGNDHLQASVGEYQVELLNKLMVRLINKYTE"
     gene            1539..3077
                     /locus_tag="PKY68_05785"
     CDS             1539..3077
                     /locus_tag="PKY68_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HOH24581.1"
                     /translation="MKEIENLELLFLSIEDYPELKEVMQMAYSSMESSYWKKHHIEKL
                     ISLFPEGQVVLKINGEIAACALSLIVQSGRFEKNHTYKQITGNYSFDTHDPLGDVLYG
                     IDVFVKPEFRGMRLGRRLYDYRKELCEKLNLKGILFGGRIPYYHNYSDKLSAREYIRQ
                     VRERKIHDPVLNFQLSNNFHPIRILKNYLEGDQASEEYAVLLKWDNIYYEEPVSKEIA
                     SPSIVKSSARLGLIQWQMRLYRDIDDLMQQAEYFIDAVSSYRADFALFPEFFNAPLMA
                     AFNHLEEPQAIRKLASFTKEIVKRFAEFSISYNINIITGSMPELVDDKLYNVGYLCKR
                     DGSVERYEKIHVTPDEAKVWGMVGGNQLKTFDTDCGKIGVLICYDSEFPELSRLLADE
                     GMNILFVPFLTDTQNGYSRIRNCSQARAIENECYVAIAGSVGNLPKVNNMDIQYAQSM
                     VFTPCDFAFPVNGIKAEATPNTEMILIVDVDLNLLTELHNNGSVRNLIDRRKDIYELK
                     RVSN"
     gene            3213..4496
                     /gene="eno"
                     /locus_tag="PKY68_05790"
     CDS             3213..4496
                     /gene="eno"
                     /locus_tag="PKY68_05790"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOH24582.1"
                     /translation="MKIKKVEAIEILDSRGNPTVEVDLKLEDGSCGRAMVPSGASTGE
                     REATELRDGDKKRYFGKGVLKAVDNVNNIIAKELEGKCFAGQRAVDYRMLELDGTPFK
                     SKLGANAILAVSMAFARAAAKSSNLPLYQYLGGSNAYLLPVPCMNIINGGQHADNNVD
                     FQEFMIAPYGATSFRESIRMGVEVFHTLKSVLNKKGYSTGVGDEGGFAPNLKSNEEAV
                     DVILEAITKAGFKPGAEVSICLDPAASELWEDGKYKMLKSSGKSLSSDELVALWADWV
                     RQYPIISIEDGLAENDWAGWKKLTDTLGNKIELVGDDLFCTNKAILKEGIEKGVANSI
                     LIKLNQIGTVTETLETIELADKNSYNNFTSHRSGETVDSFIADLSVAVNAGHIKTGSG
                     CRGERIEKFNQLIRIEKELGTAGRFAGIAAFKNAK"
     gene            complement(4588..>4954)
                     /locus_tag="PKY68_05795"
     CDS             complement(4588..>4954)
                     /locus_tag="PKY68_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOH24583.1"
                     /translation="VLVTENMFGDILTDEASVITGSLGMLPSASIGLHTSVYEPIHGS
                     YPQAAGKNIANPFAAVLSAAMMFEQTFGLSEEGKLIREAVTVAMDAGYVTEDITTGIF
                     KKARSTAAVGRWLVKYIRK"
BASE COUNT         1388 a         1223 c         1118 g         1225 t
ORIGIN      
        1 aatgctcgga atggcaaact ccagcaggag cctgttgata ctttccttac ccagcctttc
       61 gtcggaaaca gaagaagtca gcagagctgc gccctgctga cttaagttac tttctgtcga
      121 attattgtta gatatctccg gggctattac cggtgaagat tgcgcctttc ccatgaactg
      181 tcaaaaactc gcgcaaaggt agtcatttat ttacaaagcc cctttattcg gtatacttgt
      241 ttataagccg aaccatcaat ttattgagca attctacctg atattcgccc acgctggcct
      301 gaaggtgatc gttgcccact ccgctgtcgt tggtgataaa cacgtaggtg ccgttggtgg
      361 tcatagccat caggcgcatc agaaattccg tttctttgtc tatgccgctg gcggtgatgg
      421 gaattatctt aatgcccttt gcggcagcga gttgcagcga ggcatgaata tcgctgataa
      481 cagcggtagt atagtgaggc ggagcatcga gcaacaaaaa tgcaatgcgg gttctggcct
      541 gttccgacca ttgcagttcg gtcagagtgg tattcagtgc tgtatgcaca gcttcgggat
      601 aatcgcctcc ggcatcggcc cgctgtgcac cgatgtagtc gatggtagta gctgtttccg
      661 cagtaaaacc ggaatgtttc accacgtatt cgtcgccctc gtcacgatag aaaacagccc
      721 cggtaaataa attcaaactg ctattgattt tcgagacgga atccagcaca tcctgcaaat
      781 cacttttgag aaattccaac tcgtcggcca tggaaccggt ggcatccacc acaaaagcca
      841 gttccacccg gttggaattt tcaccggcac tactcaggct gatttcgttc caaccctgat
      901 caaaactttt aagctcgcct gtccaggctt tgccgtcgat gtaaagcaca taatcgctca
      961 tcgaaattct ttcggttttt tgaaacagcg atatccacat ttccgctcta cccaggttgt
     1021 cggtgcgggc tttccaaacg ctgtattccg cctttttcaa ttccaaagct gcatttatga
     1081 cgggatttgc tccatggttg atccgtatgg cgacacgatg cttggggtaa aacttccagt
     1141 aaggcggata ggtgttgtaa atagtgtcgt tgatcagatc ggtccaaaaa gtccagttat
     1201 ccagatcgtt ccactcggct gccgttacta ctccggcctg agccccgccc gaaccctgat
     1261 tattacccgg gctgcccgta ccgtcagcgt attcggcaga atgcttcccc gcaggcgtta
     1321 tatcaatccc atattcacaa gcgacaaaga acgccaacaa gggaagtaaa agcaaaagtt
     1381 tggaaatttt tttcataatg atatgttttt aaatattgaa aaaagactac gaacaaatag
     1441 atgaaaaaag agccgggacg ctacgaaaaa gccatagtct ttttcggatt tttgcgaaat
     1501 aaatcttatc tttgtgtttg atttttttaa aacataagat gaaagaaata gaaaacctgg
     1561 aacttctttt tttaagtata gaggattatc cggaactgaa agaagttatg caaatggcct
     1621 actccagcat ggagagctct tactggaaaa aacaccatat cgaaaagctg atcagccttt
     1681 ttcccgaagg ccaggtggtg ttaaaaatta acggcgaaat tgcggcctgc gcactttcgc
     1741 tgatcgttca aagcgggcgt ttcgaaaaaa accataccta caaacaaatt accggtaatt
     1801 acagcttcga cacccacgat cccttgggcg atgtgcttta cggcatcgat gtatttgtca
     1861 aacccgagtt cagaggcatg cgcctgggcc ggcgcttgta cgactaccgt aaggaattat
     1921 gcgaaaagct caatctcaaa ggcattttgt ttggcggccg cattccctat taccataact
     1981 attccgacaa attatcggcc agagaataca tccgccaggt gcgcgaacgc aaaattcacg
     2041 atccggtttt gaattttcag ttgtccaaca attttcaccc catacgcatt ctcaagaatt
     2101 acctcgaagg cgatcaggcc agcgaggaat acgccgtttt gctcaaatgg gacaatattt
     2161 attacgaaga gccggtaagc aaggagattg ccagtccctc gatcgtcaag agttctgccc
     2221 gtctcggact gattcagtgg caaatgcgtc tctacagaga catcgacgac ttaatgcaac
     2281 aggctgaata ttttattgat gccgtttcga gttaccgggc cgatttcgca ctctttcccg
     2341 agtttttcaa tgctcctctg atggcggctt tcaatcacct ggaagaacct caggccatca
     2401 ggaaactagc cagtttcacc aaggaaatcg taaagagatt tgcggaattt tccatttcat
     2461 acaacatcaa tattatcacg ggcagtatgc ccgaactggt cgacgataaa ctctacaacg
     2521 tgggttatct ttgtaaacgc gacggctcgg tggaacgtta cgaaaaaatt catgtcacgc
     2581 ccgacgaggc caaagtctgg ggcatggtgg gcggtaatca gcttaagacc ttcgacaccg
     2641 actgcggcaa aatcggcgtt ttgatctgct acgactctga attccctgag ctcagccgcc
     2701 tcctggccga tgaaggaatg aatattttgt ttgttccctt cctcacagat acccaaaacg
     2761 gctattcgag aatcaggaat tgttctcagg cccgggccat cgaaaacgaa tgctacgtag
     2821 ccatcgccgg cagcgtgggt aatttgccca aagtaaacaa tatggacatt caatatgctc
     2881 agtctatggt gtttactcct tgcgattttg cctttccggt gaacggcatc aaagccgaag
     2941 ccacacccaa tactgaaatg atcctgattg tagacgtgga tctcaatttg ctgactgaac
     3001 tgcacaacaa cggcagcgta cgcaacctga tcgaccgtcg caaagacatc tatgaactga
     3061 aacgggtttc gaactaagat ggggaaataa agcccaaaaa tcaattgtat ccgcttagat
     3121 gatgaataaa aaatatatat ctttgcgatc gtttcttcaa atatgtaatt atcccgtaaa
     3181 ataaaatcat aaactaaaaa ctagaaaaga gtatgaaaat caagaaagta gaagccattg
     3241 aaattttgga ttccagaggc aatcccaccg tagaggttga tcttaaattg gaagacggta
     3301 gttgcggcag agcaatggtg cccagcggtg cctccaccgg cgaacgcgaa gccaccgaat
     3361 tgcgcgacgg cgacaaaaaa cgctatttcg gcaaaggtgt actcaaggca gttgacaatg
     3421 tcaacaacat catagccaaa gaactcgaag gcaaatgttt tgccggacaa cgcgctgtag
     3481 attaccgtat gctggaactt gacggaaccc ccttcaaatc aaaactgggt gcaaacgcca
     3541 ttctggccgt atcgatggcc tttgcccgtg cagcagctaa aagcagcaat ttacctctgt
     3601 atcaatacct tggtggaagc aacgcttacc tccttcccgt tccctgtatg aacatcatca
     3661 atggcggaca acatgccgac aataatgttg attttcagga attcatgatt gccccctacg
     3721 gagctacttc ttttagggaa tctatccgca tgggcgttga ggttttccac accctcaaat
     3781 ctgtcctcaa taaaaaggga tacagcaccg gcgttggaga cgaaggcggc tttgctccca
     3841 acctcaagtc caacgaagaa gctgtagacg ttattctcga agccatcacc aaagccggtt
     3901 tcaaacccgg agccgaagta agcatttgcc tcgaccctgc cgccagcgaa ctctgggaag
     3961 acggtaagta caagatgctc aagagcagcg gcaaatcact aagcagcgat gaactggtag
     4021 cactttgggc cgattgggta agacaatatc ccatcatctc catcgaagac ggtttagccg
     4081 aaaacgactg ggccggttgg aaaaaactga ccgacaccct cggcaataaa atcgaattgg
     4141 tgggcgacga cttgttctgc accaacaaag ccatcctcaa agaaggcatc gaaaagggtg
     4201 tagccaactc catcttgata aaactcaacc agatcggaac ggttaccgaa actctcgaaa
     4261 ccatcgaact ggccgacaaa aactcttaca acaactttac ctcgcaccgc agtggcgaaa
     4321 ccgtcgacag ctttattgcc gacctaagtg tagccgtcaa cgccggacac atcaaaaccg
     4381 gtagcggttg ccgtggcgaa cgcatcgaaa aattcaacca gcttatccgc atcgaaaaag
     4441 aattgggtac cgccggtcgt tttgcaggca ttgctgcctt taagaatgct aaataattag
     4501 ttgtacggaa ataagaagga atccttaaac tgaaacaagc cgggtgatta ttcatccggc
     4561 ttgtttgttt tggcagtaaa aagcaattta tttcctaatg tacttcacca gccacctgcc
     4621 cacagctgcg gttgaacgtg ccttcttgaa gatcccggtg gtaatgtctt cggttacata
     4681 acccgcgtcc atggccacgg taaccgcttc tcgtatcaac ttgccctctt cgctcaggcc
     4741 aaaagtctgt tcaaacatca tggctgccga caacacggcg gcaaaaggat tggctatatt
     4801 tttgcctgct gcctgcggat aagagccgtg tataggctca tacaccgatg tatgcaaacc
     4861 tatggaagcc gacggcaaca tgcccagcga gccggtaatg accgaagctt cgtcggtgag
     4921 aatgtcgcca aacatatttt cggtaaccaa tacg
//
LOCUS       DAOSGL010000158         4934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15232_length_4934_cov_4.206190, whole genome shotgun sequence.
ACCESSION   DAOSGL010000158 DAOSGL010000000
VERSION     DAOSGL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15232_length_4934_cov_4.206190"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="PKY68_05800"
     CDS             complement(<1..1679)
                     /locus_tag="PKY68_05800"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOH24584.1"
                     /translation="MKAYYEHISSPPKHKSPCIGLSANFDDKQALLAKAYFEALTLAG
                     GSPLIIPPFKGKKQVESLLDRLDAIIFTGGGDFDPALLGEEPHEALGSFNSVRDEFEL
                     RLLKLALDKQLPILGICRGIQLINLGMGGSLYQDIYSQRRKQCINHNQTGERSETSHY
                     VELMPDTLLRSFFNKDELAVNSFHHQAIKELAGGLKVCALSSDGIIEAVESDQGKAIL
                     GLQWHPECLVSHSKEPMLPVFNWLVKEAGLYRNCRDLCQSNITLDSHCDTALYFTKEQ
                     HIEVELYSPGFDFYSGISANNANGEALSSIPENKVDLRKMQNGGLDCAVMAAYIKQGS
                     RDRQGLEKAGQTCRNMLESIQKQVANYPHLAGLARTAEDIRKLKSQGKKAILLAIENA
                     YALGTDLNLLKEFDKAGVCYITLCHNGHNDVCDSASDENNPEHHGLSPFGKELVKSMS
                     AMGILPDVSHASEKTFYDVLEYCPAPVIASHSSAKALCKHPRNLSDDQILALGKHKGY
                     IGICLYEGFLAKNRRAGIRDVIAHINHIRSLAGVEIIGIGSDFDGGGGIPG"
     gene            1942..2955
                     /locus_tag="PKY68_05805"
     CDS             1942..2955
                     /locus_tag="PKY68_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOH24585.1"
                     /translation="MNNLFVYCEIEERQIAEVSLELLTKGRKLADELSCKLEAVVIGH
                     ELDEISSQVLPYGVDVLHLFDHKTLYPYTTLPHASILIKLFKEERPQICLMGATVIGR
                     DLGPRVSSALRSGLTADCTALEIGSHKERDKTYEELLYQIRPAFGGNIVATIVNPECR
                     PQMATVREGVMKKEILDPGYRGKVIKHKVEDYVSDSDFVVKVIDRHIEKSKNKLNSAS
                     IIVSGGYGVGSKENFQLLYDLAAVLNAEVGASRAAVDAGFCEHDRQIGQTGLTVRPKL
                     YIACGISGQIQHLAGMQESAIIISINSDRSAPINSIADYVVVGRVEDVIPKMIQYYKK
                     NTK"
     gene            2958..4682
                     /locus_tag="PKY68_05810"
     CDS             2958..4682
                     /locus_tag="PKY68_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOH24586.1"
                     /translation="MGNYFTDNPVLKHYLQHPLMQRIMELKERNFSDAAAYDYAPLDA
                     EDCMDSYEKVLEICGEICADILEENAEVIDQEGPVCKNGRVEYAEGTLRNLEATVKAG
                     LMGMAVPRRYGGLNFPVTPFIMAAELISRADAAFENIWGLQECAETLYEFGTEEQRQA
                     YLGRVCAGETMSMDLTEPDAGSDLQAVMLKATLDESDNTWRLNGVKRFITNGDADIHL
                     VLARSEEGTRDGRGLSMFIYDKRNGGLTVRRIENKMGIKGAPTCELVFKDAKAELCGN
                     RRLGLIKYVMALMNGARLGIAAQSVGLCEATYRGALSYALSRKQFGKAIIEFPPVYEM
                     LALMKAKLDATRSLLYETSRFVDIYKCLEDIARERNLSPEERTEMKTYSRWADAYTPL
                     AKGMGSEFANQNAYDAVQIHGGSGYMKDYACERWYRDARITSIYEGTTQLQVIAAIRY
                     VTTGAYLEKIKAYQQEEVFPEFSELRQRLLAMTAKYEEMVAYVTGVQQDSFLDFCARR
                     LVEMAGHIIMSELLLKDAATAESLRSSAKVYVQYAESEISRHETYIRGLDVKEEAYLE
                     AFRPCCDL"
     gene            complement(4646..>4934)
                     /locus_tag="PKY68_05815"
     CDS             complement(4646..>4934)
                     /locus_tag="PKY68_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOH24587.1"
                     /translation="IGRLIPAVRQLISIPAGLAKMRFGIFLLFTALGAGLWNVILAVI
                     GYSLHAVVPKDQLMEKVYEYSHEIGYVFIGLGVLLVGFLVYKSQQGRKASK"
BASE COUNT         1292 a         1108 c         1286 g         1248 t
ORIGIN      
        1 caaccgggta tgccgccgcc gccgtcaaaa tcgctgccta tgccgattat ttcaacaccg
       61 gccagtgagc ggatatggtt gatgtgtgca ataacatccc tgatccccgc ccgccggttt
      121 ttggccagaa aaccttcata aaggcagatg cctatgtaac ccttatgctt gccaagagcg
      181 agtatctgat cgtcactaag gttgcgggga tgcttgcaca aggccttggc ggaggagtga
      241 gaagcgataa ccggagccgg acaatactca agcacgtcgt aaaaggtctt ttcggaagca
      301 tgcgagacat cgggcaagat acccatagca gacatggatt tgaccagctc tttcccaaac
      361 ggactcaatc cgtggtgctc gggattattt tcgtcggagg cggaatcgca tacgtcgttg
      421 tgtccgttgt ggcaaagggt gatgtaacac acgccggcct tgtcaaattc cttgagcagg
      481 ttcagatcgg tccccagagc gtaggcgttt tcgatggcca gcaggatggc ttttttacct
      541 tgggacttga gtttcctgat atcttcggca gttcgggcga gtccggctaa atgaggatag
      601 tttgcgacct gcttctgaat gctttccaac atattccggc aagtttgccc ggccttctct
      661 aatccttgcc tgtcgcggct tccctgtttg atataggcgg ccatgacagc gcaatcgagc
      721 cctccgtttt gcatttttct taaatccact ttgttttcgg gaatagaaga gagagcttca
      781 ccgttggcgt tgttagcgct gataccggaa taaaaatcaa aaccggggga atacaattcc
      841 acttcgatgt gttgttcttt ggtaaaatag agagccgtgt cgcaatgtga atccaaggtg
      901 atgttgcttt ggcacagatc gcgacaattc ctgtacagcc ctgcttcttt aaccagccag
      961 ttgaaaacgg gcagcatggg ctctttgctg tgtgatacca ggcattcggg atgccattgc
     1021 aagcccagaa tggctttccc ttgatccgat tccacagctt cgattatacc atctgagctt
     1081 aaggcgcata ctttcagtcc tccggccaat tctttgatag cctggtgatg aaaagaattt
     1141 acagccagtt cgtctttgtt gaagaaggaa cgaagtaaag tgtccggcat aagctcaaca
     1201 taatgagagg tttccgatcg ttcgccggtt tggttgtggt tgatgcactg tttgcgacgc
     1261 tgactgtaga tgtcctgata aagggagcct cccatgccta aattgataag ttgtattcct
     1321 ctgcaaatgc cgagtatggg aagttgttta tccaaagcca atttcaggag ccgtaattca
     1381 aattcatcgc ggacggaatt gaaactgccc aaggcctcgt ggggttcttc tcccaataga
     1441 gccggatcaa aatcacctcc gccggtaaaa ataatggcat ccagcctgtc cagcagactt
     1501 tcgacttgtt ttttgccttt gaagggcgga ataatcagcg gagagccacc ggctagagtc
     1561 agcgcttcaa aataagcttt ggccagcaaa gcctgcttgt cgtcaaagtt tgccgacaag
     1621 cctatacatg ggcttttgtg cttaggagga gaagaaatat gctcgtaata ggctttcatg
     1681 gagtgttcgt aaatgaagct tgcaccaaat agttgattgt ttctgtgcaa agataagcaa
     1741 agacggcaat tacaaagacc ggtgtcagga gattttgctg taagccgaaa ttctataaaa
     1801 aatcaaaaca gccgtcgcca agcggaaaat taatagtcaa atctcttgta gatttgacaa
     1861 aaaattgtga atcttgcatg ccttgaatcg gagaggcgtt tttctccggc agagtattgt
     1921 tttagttata cccttttagg catgaataac ttgtttgttt attgcgaaat tgaagagaga
     1981 caaatcgcag aagtgagttt ggaattgctc accaaaggcc ggaaattggc cgatgaactc
     2041 agctgtaaac ttgaagcagt cgttattgga catgagttgg acgagattag cagtcaggtt
     2101 ttgccttacg gcgttgatgt tttgcacttg tttgatcaca agaccttgta tccctacacc
     2161 accttgcctc acgcttcgat cttaatcaaa ttgtttaagg aagaaagacc tcagatctgt
     2221 ctgatgggcg caacggttat tggccgcgat ctgggcccca gagtttcttc tgctttgaga
     2281 agcggactga cagccgattg tacagccctt gaaatcggaa gtcacaaaga acgtgataaa
     2341 acctatgagg aattgctgta tcaaatccgt ccggccttcg gaggtaacat tgttgctacc
     2401 atcgtcaatc cggaatgccg tcctcaaatg gctacggtac gggagggggt tatgaaaaag
     2461 gagatactcg accccggcta cagaggcaaa gtcatcaaac ataaagtaga agattatgtg
     2521 agcgacagcg actttgtcgt taaagtgata gaccgtcata tcgaaaagag caaaaacaag
     2581 ctcaacagcg cttctattat tgtttcagga ggctatggcg ttggttcaaa agaaaatttt
     2641 caacttttat acgatttggc cgcggtgctt aatgccgaag tgggtgcctc cagagccgcc
     2701 gtcgatgccg gtttttgtga acatgaccgg caaatcggac aaaccggcct gacagtcagg
     2761 cccaagttgt atattgcttg cggaatctcc ggtcaaatac aacatttggc cgggatgcag
     2821 gaaagcgcga ttattatttc catcaacagc gatcgttccg ctcccataaa cagtattgcc
     2881 gattacgtcg ttgtgggcag agtggaagac gttatcccca aaatgattca gtattacaaa
     2941 aaaaacacta aatagccatg ggcaattatt ttaccgacaa ccctgtactt aagcattatt
     3001 tgcagcatcc gttgatgcaa cggataatgg aactaaaaga acgtaatttc agcgatgccg
     3061 ctgcttatga ctatgctccc ctagatgcgg aagattgtat ggacagctac gaaaaagtgc
     3121 tggaaatctg tggagagatt tgtgcggata ttttggaaga gaatgcagag gttatcgatc
     3181 aggaggggcc ggtgtgcaaa aacggccggg tagaatacgc agagggcacc ttgcgcaatc
     3241 tcgaagctac cgtcaaggcc ggccttatgg gcatggctgt tcccaggcgc tacggtgggc
     3301 tcaatttccc ggtaactccg ttcatcatgg ccgcggaact gattagccgg gccgatgccg
     3361 cttttgaaaa tatttggggt ttgcaggaat gtgccgaaac cttgtacgaa tttggaaccg
     3421 aagagcaaag acaggcctat ctgggacgag tttgtgcagg agaaaccatg tcgatggacc
     3481 ttactgagcc cgacgccggc tccgatctgc aggcggtgat gctgaaagcc accctggacg
     3541 aaagcgacaa cacctggcga ttgaacggcg tgaagcgttt tatcaccaac ggagatgccg
     3601 atattcacct agtcttggcc cgctccgagg aaggcaccag agacggtcgc ggattgtcga
     3661 tgtttattta cgacaaacgc aacggaggct tgactgttcg tcgcatcgaa aacaagatgg
     3721 ggattaaggg cgcgcctact tgtgagttgg tctttaaaga cgctaaagca gagctctgcg
     3781 gtaaccgcag actggggctg atcaaatacg taatggctct gatgaacgga gccaggcttg
     3841 gaattgccgc tcaatcggtg gggctttgtg aggccactta ccggggagct ctctcttatg
     3901 cactttcacg caagcaattc ggcaaagcca tcattgaatt cccgccggtt tatgaaatgc
     3961 tggcattgat gaaggccaaa ttggatgcta cacgcagtct tctgtacgaa acttcgcgtt
     4021 ttgtggatat ttacaaatgt cttgaagata ttgcaaggga gcgcaacttg agtcccgaag
     4081 aaagaaccga gatgaaaacc tattcccgtt gggctgacgc atatacccct ctggctaagg
     4141 ggatgggtag tgaatttgcc aaccagaatg cttacgatgc tgttcagatt cacggtggtt
     4201 ccggttatat gaaagattat gcctgcgagc gttggtaccg tgatgcccgt attacctcta
     4261 tttatgaagg aacaactcag ttgcaggtga ttgctgccat acgttacgtg acaaccggcg
     4321 cctatttgga gaagattaaa gcttatcaac aggaggaagt gtttcccgaa tttagcgaat
     4381 tgcgtcagcg cttgctggca atgaccgcta aatatgagga aatggtcgct tacgttacag
     4441 gagtccaaca ggacagtttt ctggacttct gtgcccgtag gttggtggaa atggcaggac
     4501 acatcatcat gtcggagttg ctcctgaaag atgccgcaac ggcagaaagc ctgcgctcat
     4561 cggccaaagt gtatgtgcaa tacgccgaat cggaaatcag caggcacgaa acctatatca
     4621 ggggtttgga tgttaaggaa gaagcttatt tggaggcttt ccggccttgt tgtgacttgt
     4681 aaacaaggaa accaaccagc agtactccca gcccgataaa aacatagccg atctcgtggc
     4741 tgtattcgta caccttttcc atcagctgat ctttggggac gacagcgtgg agtgagtagc
     4801 cgattacggc aagaataaca ttccagagac ctgccccaag ggcggtaaag agcagaaaga
     4861 tgccgaacct catttttgcc aggccggcgg ggatggaaat tagctgtctt acggcgggaa
     4921 tcaggcgtcc gatc
//
LOCUS       DAOSGL010000159         4931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15249_length_4931_cov_3.041222, whole genome shotgun sequence.
ACCESSION   DAOSGL010000159 DAOSGL010000000
VERSION     DAOSGL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15249_length_4931_cov_3.041222"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..996
                     /locus_tag="PKY68_05820"
     CDS             364..996
                     /locus_tag="PKY68_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH24588.1"
                     /translation="MHIQPFCFNPIQVNSYLLWDDDLNALIIDPGCYSNREKNTLQEF
                     IQNNKLILRAILNTHLHFDHILGNAFVENTYAVGSQAHKDDLFLLNQLPLHAQLFGVT
                     AREICRQPVALLEDGDLVHCGNFTLKAIHSPGHSPGSLVFYCENEKCLFSGDVLFRES
                     IGRSDLQGGNGNILFSSLKKLMELPHDTVVYPGHGPQTTIEYEKLHNPFL"
     gene            complement(1047..4079)
                     /locus_tag="PKY68_05825"
     CDS             complement(1047..4079)
                     /locus_tag="PKY68_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24589.1"
                     /translation="MMKKVPGLILLCFCLVVKAVAQFYSGGTDPARLKWQQLETENFK
                     LIYPVGIDSLALRYAWLLQTAGIHSIEGLRVDAPKKLPVILHPYTTRSNGFVVWAPKR
                     MELFCLPSADGEPQNWEISLALHESRHVGQMQWAGKGLFSKLYWLMGEQSQGLAAGLF
                     DMFSVGLLEGDAVMAETALSAAGRGRQAEFLMPYKAYFMDSVHFSLDKWHFGSQKHFI
                     PNEYALGYLKLSAGAFLNSSSFAADTVVTASFAGGTASSEMIFPETASSTTAAVFRDI
                     TRNPFHYNKTYKQHFGANFYRLFKPAVAFYRQMWLNEAAEKAPFDSLHLIGPVTNQYV
                     SYSSPCFSPQGLLVLKKSLSEPLSLVRIDENNKEQRLRTMGELNSSLEACGNKVYWTE
                     QINSPRWTHESYSILLSYDLEKKKIQRLSKKSRYYFPALCEDGGRVAVASYLIGGGSE
                     LHILNASNGEIKQVFSVPFGAQLYEMTWDSSGKRIFAVLIRNNGAGIYTLDTPTGNWK
                     ELMPPQHRKLSGLKYSKETLSFKSDAGGTDNIFRYNLSTGKTYRLTNTRFGAFDYLWN
                     KKEDSLYYISYDTKGYRLAVLAARELNIEEQDFKAVLPGRRVDAVLPDGSNVAGLPEN
                     PMNLAALFTAESGLVADTMVFPQDFSYRISSYSKERNLFRIHSWAPFYYDLDELSALS
                     FESLHRSLSPGLMFMSQNDLSTSVLQAGYAYRRGFHSGHLKYAYSGWYPVLSVEAHIN
                     DRQVIRQGLSYPDAEGRRTLSRDTLSAVALDTKFSAYVPLNFDRYGWHRGVVPSVNWQ
                     FLNNRYFIGSRYQSHYFNVVQAGLQLYSVKSMAPRDLFPRLGAGLNLQLAATPINRGY
                     FTDLYYAKCYAYLPGLFKNQGLKLTAAYQLHHTKNISFYLGNAVKLRGVDYINPTPKM
                     MAFSLDYALPVFPDLSLPGFLYLKRMEFYPFVDLLKTLPETTTGIESESLYSSGLETL
                     LNVHLLRINVELNLGLRCSYNPAKGMVYEMLFSLPYLN"
     gene            complement(4079..>4931)
                     /locus_tag="PKY68_05830"
     CDS             complement(4079..>4931)
                     /locus_tag="PKY68_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HOH24590.1"
                     /translation="IGKLNWDDFLNLEISGRQAQLSQLSIEEANEVPVVYLCGNSTVV
                     DQDNEPWASWGQMITVFFDDRLCFANYAESGESANSFISAGRLKKIMTQIKPGDYVLV
                     EFGHNDQKQKGEGKGAYLSYWKSLTGFIDQAREKKAIPVLITPTQRRSFGDDGRIVDT
                     HEDYPQAMRDLAREKQVPLIDLHQMTRVLYEALGTEGSKKAFVHYPAGSFPGQNQDLA
                     DNTHFNPYGAYQIAKCVVMGMKQLGLPLVQFLREEVVYDPAFPDDPGRFNWWPSPYVE
                     LQKPEGN"
BASE COUNT         1358 a         1220 c         1070 g         1283 t
ORIGIN      
        1 aacgcatacg tacttgtctc aacttttgcc ctatggcata acttaattct gtgtttgtaa
       61 tctttctgga catatcaata atggttaatg tattcggaat gcaaatataa tatattgttt
      121 acatacgcaa aacattttat gattattatt tttttctctg tatttccatt ttcttcgttt
      181 ccgttttttg tatttccttt tttcatccct ttttttattt ctgtttcttc gccctgcttc
      241 tgggagttcc agtcttcttc actctactca caggttttac cttcgaaccg atgaatgata
      301 aacaagccaa aaaagaagtt ttgagaatta tttgatacct ttgccctttc attttcggcc
      361 attatgcata ttcaaccgtt ttgcttcaat cccatacagg tgaacagcta tttgctttgg
      421 gacgacgatc tcaatgcctt aatcatcgat cccggctgtt attcgaacag ggaaaaaaat
      481 accctacagg aattcatcca aaacaacaag ttgattttga gagcaatatt gaacactcac
      541 cttcatttcg accatattct gggcaatgcc tttgtcgaaa atacctatgc cgtgggcagc
      601 caggcacaca aagacgattt attcctcttg aatcaactgc cgctgcatgc tcaactcttt
      661 ggcgttacgg cccgggaaat ttgccggcaa ccggtcgctt tgctcgaaga cggtgatttg
      721 gtacattgcg gtaatttcac tctcaaggcc attcactcac ccggccattc gccgggcagc
      781 ctggtttttt attgtgaaaa cgaaaaatgc ctcttctcgg gcgacgtctt attcagagaa
      841 agtataggac gcagcgatct gcagggagga aacggtaaca tactgttttc gagtcttaaa
      901 aaactgatgg aactgcctca cgacacagtc gtttacccgg gacacggacc ccaaaccact
      961 atagaatacg aaaagctgca caatcccttc ctttgatctg tgccgggttg aaaaaccaaa
     1021 ccccgaaaaa tccccgatcg gcagtattaa ttcaaataag gcaaagagaa cagcatttcg
     1081 taaaccatgc ctttggcggg attgtaagag cagcgtaagc cgaggttaag ttctacgttg
     1141 atgcgcagca gatgtacatt caacaaagtt tccaggcctg atgaatagag gctttcgctc
     1201 tctataccgg tcgtagtttc aggcagggtt tttaacagat ccacaaaagg ataaaactcc
     1261 atacgcttca aataaagaaa accgggcagc gaaagatcgg ggaaaacagg caaagcataa
     1321 tccaaactaa aagccatcat cttaggggtc ggattaatgt agtcgacacc acgaagttta
     1381 acggcattac ccaaatagaa acttatgttt ttggtatggt gcagctgata agctgccgtc
     1441 aatttcagac cctgattttt gaataagcca ggcaaataag cataacattt ggcatagtac
     1501 agatcggtaa aataaccacg gttaatagga gttgcagcca actgcaaatt cagaccggct
     1561 cccaggcggg gaaacaaatc acgcggagcc atcgacttta cactataaag ttgcagccct
     1621 gcctgtacga cattgaaata atgactctga tacctggaac cgataaaata ccggttattt
     1681 aaaaattgcc aattcacgga aggcacaaca cccctgtgcc agccatatct gtcgaaattc
     1741 aaaggcacat aggcagaaaa tttggtgtct aaagccacag cggagagtgt gtctctgctc
     1801 agggtccgtc ttccttctgc gtccggataa ctcaatcctt gtctgataac ctggcgatcg
     1861 ttgatatggg cttcgacgct caacacggga taccagccgg aataggcgta tttaagatgg
     1921 cctgaatgaa atccgcggcg ataggcatag ccggcctgca gcaccgaggt actgagatcg
     1981 ttctgcgaca taaacatcaa cccgggactc aggctccggt gcaggctttc aaaacttaat
     2041 gcggacagtt cgtccaagtc gtaataaaag ggcgcccagg aatgtatccg gaacaaattt
     2101 cgctccttgg aatagctgct gatccgataa ctaaagtctt ggggaaaaac catggtatcg
     2161 gccaccaaac cgctctctgc cgtaaaaagg gcggccagat tcattgggtt ctccggtaat
     2221 ccggctacgt tagagccatc gggcaagacc gcatcaacac gcctcccggg taacacagcc
     2281 ttaaaatctt gttcctcgat attcaactct cgtgccgcca gtaccgcaag ccggtaccct
     2341 tttgtgtcgt aagaaatata atacaggctg tcctccttct tattccagag ataatcaaaa
     2401 gctccgaaac gggtattggt caaacgatac gttttccctg tggacagatt ataacggaaa
     2461 atattgtcgg tgcctccggc atcgctctta aaactgagtg tctctttgga atattttaaa
     2521 ccggacaatt tgcgatgttg cggaggcatc aactctttcc aatttccggt cggagtatcg
     2581 agcgtgtaaa taccggcccc gttgttacgg atcaaaacgg caaaaatgcg ctttcccgag
     2641 ctgtcccagg tcatttcgta caattgggcc ccgaaaggaa ccgaaaaaac ttgcttgatt
     2701 tctccgttag aagcattcaa tatatgcaat tcggagcctc cgccgatcag ataagatgca
     2761 acagcgaccc gacctccgtc ttcacacagc gcaggaaaat aatagcgact ctttttactg
     2821 agcctctgaa ttttcttctt ttccaaatcg taactcaaca aaatggaata gctttcgtgt
     2881 gtccaacgcg gactattgat ttgctccgtc caataaacct tgttcccaca ggcttccaaa
     2941 gaagaattga gttcccccat agtgcgcaga cgctgctctt tgttgttctc gtctatccga
     3001 actaaactca aaggttcgga caggcttttt tttaggacca acaggccttg agggctaaaa
     3061 caaggagaac tgtatgagac gtattgattt gtgaccggac cgatcagatg caagctgtcg
     3121 aaaggagcct tttctgccgc ctcgttcagc cacatctgcc ggtaaaaggc aacagccggt
     3181 ttaaacaagc gataaaaatt agcaccgaaa tgttgtttgt aggttttatt gtaatggaag
     3241 ggattacggg taatatccct aaataccgca gctgtcgtag aagaggccgt ttcagggaaa
     3301 atcatttcag atgaagcagt tccaccggca aaagaagcag ttacaactgt atcggcagca
     3361 aaagaagaag aattcaaaaa agcgccggcc gaaagtttta ggtaacccag cgcgtactcg
     3421 ttgggaataa aatgtttttg cgagccgaaa tgccatttgt ccaaagaaaa atgcaccgaa
     3481 tccataaaat aagccttgta gggcatcaga aactctgcct gacggcccct tccggcagca
     3541 gataaggctg tttcggccat aacggcatcg ccttcgagca aacccacaga aaacatatcg
     3601 aacaaaccgg cagctaatcc ttgcgactgt tctcccatta accaatacaa cttagagaac
     3661 aggcccttac ccgcccattg catttgaccc acatgcctgg attcgtgcaa agccaggctg
     3721 atttcccagt tctgaggctc gccgtcggcc gaaggcaggc aaaacaattc cattcttttg
     3781 ggcgcccaca ccacaaaacc attgctgcga gtagtgtaag gatgcagtat gacgggaagt
     3841 tttttaggag catccacacg caagccctct atgctatgta tgcccgccgt ctgcaacaac
     3901 caggcatatc gtaaagccag cgaatcgata ccgacgggat aaatcagctt gaaattttca
     3961 gtttcgagct gttgccattt aagacgggcc gggtccgtgc ctcccgaata aaattgagca
     4021 actgctttta ccaccaggca aaaacacaac aatatcaggc ccggtacttt tttcatcatc
     4081 aattgccctc gggtttctga agttccacat agggagacgg ccaccagtta aacctgccgg
     4141 ggtcgtccgg aaaagccgga tcgtaaacca cctcttccct taaaaattga accagcggca
     4201 aacccagttg cttcattccc attactacgc atttggcaat ctggtaggcg ccgtagggat
     4261 tgaaatgggt attgtcggcc aggtcctgat tttgtccggg aaagctgcct gccggataat
     4321 ggacaaaggc ctttttggaa ccttccgtcc ccagcgcttc gtacaaaacc ctggtcattt
     4381 gatgtaaatc aatcaggggc acctgttttt cgcgggccaa atcgcgcata gcctgaggat
     4441 aatcctcgtg agtgtcgact atccggccgt catctccaaa actgcggcgc tgcgtgggtg
     4501 taatcaatac cggtatggcc tttttttcgc gagcctgatc gataaacccg gtcaagcttt
     4561 tccaatacga aagataagcc cctttgccct ctcctttttg tttttgatcg ttgtgtccga
     4621 attccaccaa tacataatcg ccgggcttga tttgcgtcat gatctttttc aatctgccgg
     4681 cgctgataaa actattggcc gattcacccg actcggcgta attggcaaaa cacagtctgt
     4741 cgtcaaaaaa aacggtgatc atttgtcccc agctggccca gggttcatta tcctgatcaa
     4801 ccacggtgga attaccgcag agatacacca ccggcacttc atttgcctct tctatagaca
     4861 attggctgag ctgagcttgg cgtccgctta tttcgagatt gagaaaatcg tcccaattga
     4921 gtttgccgat t
//
LOCUS       DAOSGL010000160         4922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15289_length_4922_cov_4.662009, whole genome shotgun sequence.
ACCESSION   DAOSGL010000160 DAOSGL010000000
VERSION     DAOSGL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15289_length_4922_cov_4.662009"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKY68_05835"
     CDS             <1..980
                     /locus_tag="PKY68_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014299566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOH24591.1"
                     /translation="GTGQSLLQHTYHRFSKIIPPENIFVVSNKLYGKLIREQLPGLSP
                     KQILLEPQRRNTAPCIAFASHHISAVNPKANIVVAPSDHVILKEDEFLEHISEGLKFV
                     KENPVLLTIGIKPNRPETGYGYIQKGHQKIASFTKAKTFTEKPDLELAKVFVESGEFL
                     WNSGIFLWNVQSILKAFEEFLPDIHSSFSAGEKLFNTPKEGEFIEKIFSSCPNISIDY
                     SIMEKAPNVYVQEGGFGWSDLGTWGSLHDLSPKDKDNNVILKCKALCYESSNNIITLP
                     EGKLAVVQKLDGYIVAESDNVLMICKKEDEQEIRQYVIDAQLKFGDSFV"
     gene            complement(1012..1809)
                     /gene="lptB"
                     /locus_tag="PKY68_05840"
     CDS             complement(1012..1809)
                     /gene="lptB"
                     /locus_tag="PKY68_05840"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOH24592.1"
                     /translation="MPEEKLVLRTENLVKRYHKRTVVDRVSIELHQGEIVGLLGPNGA
                     GKTTTFYMTTGLVVPNEGRIFLGEVDITDYPVYKRAQAGIGYLAQEPSVFRKMSVEDN
                     ILSVLEMTKMTRAEQKEKLESLIAEFHLEKVRKNIGDRLSGGERRRTEIARCLAISPK
                     FIMLDEPFAGVDPIAVDDIQQIVAGLVTKNIGILITDHNVRETLSITDRAYMLFEGKI
                     HFQGTPEELASNEVVRRKYLGVNFELTRKRKPVKKSSVAESNRTAQK"
     gene            complement(1855..3528)
                     /locus_tag="PKY68_05845"
     CDS             complement(1855..3528)
                     /locus_tag="PKY68_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH24593.1"
                     /translation="MKKLYGRLGFVVLLLSVGIFAIKADTIPAFPGAEGYGRYVTGGR
                     GGTVYKVTNLKDDGSEGSLRWAVNKSGPRTIVFDVSGTIYLKSNLNINNGNLTIAGQT
                     APGDGICLRDYTVQVSADNIIIRFMRFRLGDVTATENDAIWGRRRSNIIIDHCSMSWS
                     TDECASFYDNTNFTMQWCVLSESLRESVHGKGTHGYCGIWGGKGASFHHNVLAHHDSR
                     NPRFCGSRYSNRPDLEKVDFRNNVIYNWGSNSGYAGEGGSYNMVNNYYKFGPATKTNV
                     RSRIFQPWPDNGKNSQPAGVTGLFYVNGNYMYGNAAVTGDNWQGIHPSSGSINTYRTD
                     TEFDFPFTTTHTAGNAFEKVLAYAGASLVRDTIDRRIMEEIRNGNYTYTGSKGGTKGI
                     IDSQEDVGGWPEYNSGSAPVDSDNDGIPDDWEDILGLDPTDAADAKRTNREGYTMLEV
                     YLNTLVSSIVNNQNADAINALPLNEFLAGNKLELQIADGRNTGIQIRANAALEKIQVY
                     DLTGRLRLHLEQIQSNECRVDATGLSPGIYILRAKSISGKEATIKLLRH"
     gene            complement(3658..>4922)
                     /locus_tag="PKY68_05850"
     CDS             complement(3658..>4922)
                     /locus_tag="PKY68_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH24594.1"
                     /translation="MLAWDFCQLTANNTRFSSLEANHYVNEANKGEMNYVTGDTLAAD
                     FQTRNNKFTAEGKELHYSALRRTPAANFDNPDYLFVKFSTLGYTHITVKSAIASDNCV
                     HLVQKMQYSLNGTDYIDFAVDTLPSAEADWNQVWFPLQGTLPKEAQNREEVYVRWIAD
                     KTSERVKLASLEEVAYEYLYISQIVILGDDGNGGASWRVKPYDYYLAGDVVEVPGIKL
                     TLGGGTNVWSVSDSVFVFDGVTYISALNGTVNPVDDNNKKFSSSGLPPTVGAFYKFEP
                     FHNGTLKVALVVNANKTSFIIEDNVALPNYNPFSFTEKTYASFDINVTGGKTYYMFSE
                     GSKMGIMGFVYRVPDDLNKAKLTTQIFANNGSLFVKLNKAESIKVYDLLGRTVKSLEL
                     REGLNEINGLSRGIYLVRLGSETVKIQL"
BASE COUNT         1247 a         1188 c         1125 g         1362 t
ORIGIN      
        1 tcggtaccgg tcaatcgctg ctccaacata catatcaccg tttcagtaaa atcattccgc
       61 ccgaaaacat ttttgttgtt tccaataaat tatacgggaa actcattcgg gagcaattac
      121 ccggcttaag tcccaaacaa attctgcttg aacctcagcg caggaatact gctccctgta
      181 tagccttcgc ttcgcatcac atcagtgctg tcaatcccaa agccaatata gtggtcgctc
      241 cttcggacca tgttattctt aaagaagatg aatttctgga acacatcagc gaggggctta
      301 aatttgtgaa agaaaacccg gtgttgctca ctataggtat taagcctaac cgtccggaaa
      361 ccggttacgg ttacattcaa aaaggacatc aaaaaatagc ctcattcacc aaagccaaaa
      421 catttaccga aaagcccgac ctcgaactgg cgaaagtgtt tgtggaaagc ggggaatttt
      481 tgtggaactc aggcattttt ctttggaatg tgcaaagtat tttaaaagca ttcgaggagt
      541 tcttacccga tatccactcc tctttcagtg ccggagaaaa attgttcaat acccccaaag
      601 agggcgaatt tattgaaaaa atattctcgt cctgccccaa tatctccatc gattacagca
      661 ttatggaaaa agcccccaat gtgtatgtac aggagggtgg tttcggctgg tccgacctcg
      721 gcacctgggg ttccttacac gatctttcgc ctaaggataa agacaacaac gtgatcctga
      781 agtgcaaggc attgtgttac gaaagcagca acaatatcat caccctgccc gagggaaagc
      841 tggctgtggt acaaaaactc gacggctata ttgtagccga atcggacaat gtgctgatga
      901 tatgtaaaaa agaagacgaa caggaaattc gccagtacgt catcgatgcc cagctgaaat
      961 tcggagattc ctttgtataa ataaggaact cgtttttaaa tgttggggaa cttatttttg
     1021 agctgtccgg tttgattcag cgacggaaga ttttttgacc ggtttccttt ttcgggtcaa
     1081 ctcaaaattc actcctaagt atttgcgacg aacaacttcg ttggaggcta attcctcggg
     1141 ggtgccctga aaatgtattt ttccctcgaa caacatataa gccctgtctg taatggacag
     1201 ggtttcgcgt acattatggt ccgtaatgag aatgccaatg tttttggtta ccaagccggc
     1261 cacaatttgc tgaatatcgt ccaccgcgat gggatcgaca ccggcaaaag gttcatcgag
     1321 cataatgaat ttggggctga tggccaggca acgggcaatt tccgtccgcc gtctttcccc
     1381 tccggacagg cggtcgccta tgtttttgcg caccttctcc aaatgaaatt cggcaatgag
     1441 gctttcaagt ttctcttttt gttctgcccg ggtcattttt gtcatctcca acaccgacaa
     1501 gatgttgtct tctaccgaca ttttacgaaa cactgagggt tcctgagcaa ggtaaccgat
     1561 accggcctgg gcacgtttgt acacgggata atcggtaata tctacctctc ccaggaaaat
     1621 acggccttcg ttgggcacta ccaagcctgt ggtcatataa aaagtggtgg tcttacctgc
     1681 accgttgggg cctagcaaac caacaatttc tccctgatgc aattcgatgg acaccctgtc
     1741 taccacggtg cgtttatgat acctcttgac cagattttct gtgcgcagca ccaatttttc
     1801 ttcgggcatg attgagcttt tgtcgcaaaa gtaaacaaaa atcggaatac tatatcaatg
     1861 cctgaggagc tttatggtag cttcctttcc cgaaatcgat ttagccctca atatgtaaat
     1921 acccggtgat aaccccgtag catctacacg acattcgttg ctttggattt gttccagatg
     1981 gagtctgagt ctgccggtca ggtcgtaaac ctgtattttt tccagagcgg cattggccct
     2041 gatttgaata ccggtatttc tgccgtcggc aatctgtaat tccagtttat ttcctgccag
     2101 gaattcattc agaggcaaag cattgatagc gtcggcgttt tgattgttaa cgatactact
     2161 gaccagcgta ttcaggtata cttcgagcat agtatagcct tctctgtttg tccgtttggc
     2221 atcggcggca tcggtaggat ccaggccgag tatatcttcc cagtcgtcgg ggatgccgtc
     2281 gttgtcactg tctacgggcg ctgatcccga attgtattca ggccagcctc ccacgtcttc
     2341 ctgcgaatct atgatgccct tggtgccgcc tttggaaccc gtataggtgt agttgccatt
     2401 gcgaatctcc tccataatac ggcggtcgat ggtgtctctt accaggctgg ctccggcgta
     2461 agccaatact ttttcaaaag cattcccggc ggtatgcgta gtggtaaagg gaaaatcaaa
     2521 ttcggtatct gtacggtaag tatttatgct tccactggaa ggatgtatgc cttgccaatt
     2581 gtctccggta acggcagcat ttccgtacat ataattgccg tttacataga acagtcccgt
     2641 aacacctgcc ggctgggagt ttttcccgtt atcgggccag ggctgaaaaa tacggcttct
     2701 cacattcgtt ttggtagccg gcccgaattt atagtaattg ttcaccatat tgtagctgcc
     2761 cccttcgccc gcgtaaccgc tgttgctgcc ccaattgtaa ataacgttat tacggaaatc
     2821 cactttttcg agatcgggcc tgttggagta gcgactgccg cagaaacggg gattgcgact
     2881 gtcgtgatgc gccagcacat tgtggtggaa actggcccct tttccgcccc aaataccgca
     2941 atagccgtgt gtacccttac catggacaga ttcccggagg ctttcactca gaacacacca
     3001 ctgcatggta aaattagtat tatcgtaaaa cgaagcacat tcatcggtgc tccagctcat
     3061 ggagcaatga tcgataatga tgtttgaacg gcgacgcccc caaatagcat cgttttcggt
     3121 ggccgtcaca tcgcccagcc tgaaacgcat aaaacgaatg ataatgttgt cggcgctcac
     3181 ttgaaccgta taatctctca aacaaattcc atctccgggg gcggtctgcc ctgcaatggt
     3241 cagattgccg ttattgatat tcaggttgct ttttaaataa atggtgcccg atacgtcaaa
     3301 aacaatggtt ctggggccgc ttttatttac ggcccagcgc agggaaccct ccgatccgtc
     3361 gtccttgaga ttggttacct tgtacaccgt gccgccccga ccaccggtga cgtagcggcc
     3421 ataaccttcg gctccgggaa aagccggaat ggtatctgcc tttattgcaa agattcccac
     3481 gctaagtaac agcacaacaa atcctaatct tccgtatagt tttttcatgg aatatgtgtt
     3541 ttatgttcgt aaagaagcaa gcctccccga atggaggctt gctctttagt ctattcagct
     3601 tgcaaagata ggaaaaatcc gctaatccaa actttgcaag caagctgatc tttaggttta
     3661 cagctgaatc ttaacggttt ccgatccgag gcgtaccaag taaatacccc ggctcaggcc
     3721 gttgatctcg tttaggcctt cgcggagttc aagtgatttt actgttctgc ccaaaaggtc
     3781 gtaaacctta atggattctg ctttgtttag ttttacaaac aggctgccgt tgttggcaaa
     3841 aatctgcgtg gtcagtttag ctttattcag gtcatccgga accctatata caaagcccat
     3901 gatgcccatt ttagaacctt cactgaacat gtaataggtt ttaccgccag taacatttat
     3961 atcaaaagag gcataggttt tttctgtaaa gctaaaagga ttgtaattgg gcaatgccac
     4021 attgtcttca ataataaaag aagtcttgtt ggcgttgact accagggcaa ctttaagtgt
     4081 cccgttgtga aagggctcaa atttatagaa ggcaccaact gtaggaggta aaccactgga
     4141 cgagaatttc ttgttgttat cgtctacagg atttaccgtt ccattcaggg ctgagatata
     4201 ggtcaccccg tcgaaaacaa acaccgagtc gctgacagac cagacattgg tgccgccacc
     4261 cagggtgagt tttatacccg gaacttcaac cacatcgccg gccaggtagt aatcgtaggg
     4321 ttttactctc cacgaagccc caccgttacc gtcgtcaccc agaatcacaa tctgcgaaat
     4381 atagaggtat tcgtaagcaa cttcctctaa actggccaat ttaactcttt cactggtttt
     4441 atcggcaatc cagcggacat aaacttcttc ccggttttgg gcttccttgg gcagggtgcc
     4501 ttgcaacggg aaccaaacct gattccaatc ggcttcggcc gaaggcaggg tgtcgacggc
     4561 aaaatcaatg taatcggtgc cgttgagcga atactgcatt ttttgtacca aatgcacgca
     4621 gttgtcggag gcaatcgccg acttaaccgt gatatgtgta tagcccagcg tggagaattt
     4681 tacgaagaga taatcggggt tatcgaagtt cgcagcggga gtcctcctta gagcgctgta
     4741 atgcagttct ttgccttcgg cggtaaattt attgttcctg gtctggaaat cagcagcgag
     4801 ggtatcgccc gtgacatagt tcatttcgcc cttgttggcc tcattgacgt aatgattggc
     4861 ttcgagcgaa ctgaatctgg tattgttggc tgtcagttga cagaagtccc aggccagcat
     4921 gt
//
LOCUS       DAOSGL010000161         4887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15426_length_4887_cov_3.792632, whole genome shotgun sequence.
ACCESSION   DAOSGL010000161 DAOSGL010000000
VERSION     DAOSGL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15426_length_4887_cov_3.792632"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKY68_05855"
     CDS             complement(<1..386)
                     /locus_tag="PKY68_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOH24595.1"
                     /translation="MINRVLIRTKVLQMVYACHQNNNNENPAKAEKELFFSLEKTYDL
                     YLYLLLLVSDIGYYAERRIDMARGKYLPSPEEQNPNLRFVNNAFLAQLRQNTTFKREI
                     NNRKLSWAPHEGLIKNIYDQILVAPF"
     gene            530..895
                     /locus_tag="PKY68_05860"
     CDS             530..895
                     /locus_tag="PKY68_05860"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOH24596.1"
                     /translation="MKTDTEDNKNIDKDMVYNKTVKAGKRIYYFDVKKSRNNEMYIAI
                     TESKKIVSGDLLTPDISFKKHKIFLYHEDMSNFAEALLQVIQYVKERQAPPPPREQYW
                     EKKKEADRSTPDEDDSLGI"
     gene            1111..1713
                     /locus_tag="PKY68_05865"
     CDS             1111..1713
                     /locus_tag="PKY68_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOH24597.1"
                     /translation="MKTLELNGSPRTDFGKKASKNVRNKGNVPCVLYGGEKNLHFQVK
                     KEDLRALIYTPAVHLIKLNIAEKAYMSILQDVQFHPVSDEPLHLDFLQIFEDKPILIE
                     VPVKLNGFAAGVKDGGKLSLDLRKLKVKALYKDIPDFLEIDITNLELGKTVQVGDLEF
                     PNMELMNTKNAVVCAVKLTRVARGIAAATAATESEESETE"
     gene            1710..2273
                     /gene="pth"
                     /locus_tag="PKY68_05870"
     CDS             1710..2273
                     /gene="pth"
                     /locus_tag="PKY68_05870"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOH24598.1"
                     /translation="MKYLIAGLGNIGETYQHTRHNIGFDILDAFSEASNVVFEPKRYG
                     AVAEGRLKNKQFILLKPSTYMNLSGNAVRYWLQAEKISLENLLVVVDDLALPFGRMRL
                     RPDGGDAGHNGLSHIQESLGTDKYARLRFGIGNDFPQGTQVDYVLSGWTAEQLETLPE
                     RIDQAVEIIKSFCLAGIQLTMTRYNRK"
     gene            2338..2724
                     /locus_tag="PKY68_05875"
     CDS             2338..2724
                     /locus_tag="PKY68_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOH24599.1"
                     /translation="MNQSVRIDKWLWSARIYKTRTLATEACRKGQVSIAGVKVKPSRM
                     VTEGEILNVRKNPLTLTLKILSPIQNRVPARLIPEVLQDLTPAEEYEMLEMARIASFG
                     KRAAGSGRPTKKERRDLESFLNSEEE"
     gene            complement(2792..3319)
                     /locus_tag="PKY68_05880"
     CDS             complement(2792..3319)
                     /locus_tag="PKY68_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH24600.1"
                     /translation="MRTFLQNEQLRLRSPEPEDLEFLYRCENDTSLWQYGSTLSPFSK
                     YALHQYISQAEQNIFVSRQLRFMIDSKKDDCVVGTIDLYDFDPYHNRAGVGILIDKAY
                     QRRGYATQALKLLIEFAFGFLHIHQLYAYIPTENSDSLALFSKLGFEQSGVLKDWNMG
                     LDKYKDIAVFQLIAP"
     gene            complement(3331..3834)
                     /locus_tag="PKY68_05885"
     CDS             complement(3331..3834)
                     /locus_tag="PKY68_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24601.1"
                     /translation="MEADLHLVLKKLRLQFFLSVGLLLCLGLLAYLFPLQSILLSQEL
                     SLPFQSFCILLFLIGVPFSLRWFHHKTRAIRLEKALKERLPAYRKAASARMYVFSLIA
                     LFALFLEVFTTMPNALVFFLMTMLFFLFCIPVKGQVIKDLELFPNPEQAQETEEQPSE
                     QASERPK"
     gene            complement(3836..4450)
                     /gene="recR"
                     /locus_tag="PKY68_05890"
     CDS             complement(3836..4450)
                     /gene="recR"
                     /locus_tag="PKY68_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOH24602.1"
                     /translation="MDQIYPSVLLEEAVQAFSGLPGIGRKTALRLVLHLLRQNKEDVE
                     HFGNTLIRLKNEIAYCKTCHNISDTEECLICSNPRRDTSIICVVENVREVMAIEQTRQ
                     YNGLYHVLGGLISPMDGIGPSDLEINSLVERLAQNRVEEVILALSATAEGDATSFYIF
                     RKISTYPLKITVIARGISVGDELEYADEITLGRSILNRIPFKTS"
     gene            4539..>4887
                     /locus_tag="PKY68_05895"
     CDS             4539..>4887
                     /locus_tag="PKY68_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOH24603.1"
                     /translation="MNEFLVLGLLLAYFGLLLLISVLSKGSDQNNNAFFLGERKSPWP
                     VVAIGMIGASLSGVTFVSVPGMVRENSFLYMQTVMGFFVGYVLIANVLLPVYYRLNSP
                     SIYVYLKERFGKYS"
BASE COUNT         1430 a         1039 c         1147 g         1271 t
ORIGIN      
        1 taaaaaggag ccactagaat ctggtcataa atatttttta tcaatccttc gtggggtgcc
       61 caggataatt ttcggttgtt tatctcgcgt ttgaaggtgg tgttttgcct gagttgagcc
      121 agaaaggcat tgttcacaaa acgcaaattc ggattttgtt cttccgggct gggcaggtat
      181 ttgccgcgag ccatgtcgat gcgtcgttcg gcataataac cgatgtctga aaccagcaac
      241 aatagatata aatacagatc gtaggttttt tctaaactaa aaaataattc cttttcggct
      301 tttgccgggt tttcgttgtt attgttctgg tggcaggcat acaccatttg taatacctta
      361 gtacggataa gaacacgatt gatcattacg gattctacaa aaagcaacgc cgcaaagata
      421 atatatttta ccaagcaaga ttgcatcctt aaaatattat atctaatttt gaactcagat
      481 caaagacaac tccggttgaa cttattatcg aatttattga tgaagtacca tgaaaacaga
      541 cacagaagat aataagaata tagacaagga catggtctat aacaaaacgg taaaagcagg
      601 aaaacgcatc tattattttg atgtaaaaaa aagccgaaac aacgagatgt atattgcgat
      661 taccgaaagt aaaaagattg taagcggtga tttacttacc ccggatatca gcttcaaaaa
      721 gcacaaaata tttctttatc acgaagacat gtccaatttt gccgaagctc tgcttcaggt
      781 aattcaatac gtcaaagaaa gacaagcacc acctccgccc cgggaacaat attgggagaa
      841 aaagaaagag gccgatcgca gcactcccga cgaggacgac agcttaggga tttaggcaag
      901 ttcgtcaaga gctttgaaga attcagcgac cggactactg ccgacaaaga cgataacgac
      961 gaagattccg gcgattcgag ctaataattt ttcaggcttg tttgttgtaa taaatctttt
     1021 gattaccttt gcgccgcttt tgactaaacc cgtgtgatgg gaaaatttaa gagcttattt
     1081 taaatttttg agtaacacaa ttcaatcaca atgaaaactt tagaacttaa tggcagcccg
     1141 agaaccgatt tcgggaaaaa agcctccaaa aacgttcgca ataaaggcaa cgtaccttgt
     1201 gtcctttacg gaggtgaaaa aaacctgcat ttccaggtaa aaaaagagga tttacgagca
     1261 ttgatttata ctcctgcagt tcatttgata aagcttaata ttgcagaaaa ggcttatatg
     1321 tcgatacttc aggacgtaca gtttcatccc gtcagtgatg agcctctgca tcttgatttc
     1381 ttgcaaattt tcgaagacaa acccatcctt attgaagtac ctgtcaaact caatggtttt
     1441 gcggccggtg ttaaggatgg cggtaagttg agccttgatt tgcgcaaact caaagttaaa
     1501 gctttgtata aagatattcc cgattttctg gaaatcgaca ttaccaactt agagttgggt
     1561 aaaacggtac aggtgggtga tctcgaattt cccaacatgg aactgatgaa caccaaaaac
     1621 gccgtggttt gtgctgttaa actgacccgt gtagccagag gtatagctgc tgccactgct
     1681 gccacagaat cggaagaaag cgaaacagaa tgaaatacct gattgccggt ttggggaata
     1741 taggtgagac ctatcaacac acccggcata acattggatt tgacatattg gacgctttct
     1801 cggaagcgtc caatgttgtt tttgagccta agcgttacgg cgctgtagca gaaggccggc
     1861 tcaagaacaa gcagtttatt ttgctgaaac cttccactta catgaatctc agcggtaacg
     1921 ccgtgcgtta ctggctgcag gccgaaaaaa tctcattgga gaatttgctg gtagtggtag
     1981 acgatctggc attgcctttt ggtcgtatgc gtttgcgtcc cgacggggga gatgccggcc
     2041 ataacggact gagtcatata caggaaagcc tgggcaccga taaatacgcc cgtctgcgtt
     2101 ttggtatagg caacgatttc cctcagggga ctcaggtgga ttacgtactc agcggctgga
     2161 ctgccgaaca actggaaaca ttgcccgaga ggatagatca ggccgtagaa atcatcaaaa
     2221 gcttttgtct ggccggcatt cagcttacca tgaccaggta caaccgcaaa taagcatgga
     2281 tcgaagcctc ccagcccgaa acgtttggct tgctgcacca gcgaaccgta aataaacatg
     2341 aatcaaagcg tacgcataga caaatggctg tggtctgccc ggatctacaa gaccagaaca
     2401 ctggccaccg aagcttgtcg caaaggccag gtgagtattg caggggtaaa agtaaagccc
     2461 tccagaatgg tgaccgaagg cgaaatactc aacgtacgca aaaatcctct taccctgacg
     2521 ctgaaaatac tgagtcccat tcaaaacaga gtccccgcaa gattaattcc ggaagttctg
     2581 caggatctga cccctgccga agaatacgaa atgctcgaaa tggcccgtat tgctagcttt
     2641 ggcaagcgag cggcaggcag cggcaggccc accaagaaag aacgtcgcga cctggaaagt
     2701 ttcctaaata gtgaagagga ataaacggca aatctaagca aatagcattc ccgtactcat
     2761 aagaccccac cttatgagca acaagctgaa tttagggtgc aattaattga aatacagcaa
     2821 tatctttgta tttatccagt cccatattcc agtctttcag cacaccgctt tgttcaaaac
     2881 ccagtttgct aaaaagagcc aggctgtctg agttttcggt aggaatgtag gcgtaaagct
     2941 gatgaatatg cagaaaacca aaagcaaatt caatcagtag ctttaaagcc tgagtggcat
     3001 agcccctgcg ctgataggcc ttatctatca gaatgcctac accggcgcgg ttgtggtagg
     3061 gatcaaaatc gtaaagatcg atggtgccga caacacagtc gtctttttta ctgtctatca
     3121 taaaacgcaa ttgcctggaa acgaaaatat tttgttcggc ctggcttatg tattgatgaa
     3181 gagcatattt tgaaaagggc gacagggtgc tgccgtattg ccagaggctg gtgtcgtttt
     3241 cgcagcggta gaggaattcc aggtcttcgg gctcgggcga ccgtaagcgc aattgttcgt
     3301 tttgaaggaa ggttctcatg gttgggggaa ttatttgggt ctctcagaag cttgctccga
     3361 aggctgctcc tccgtttcct gagcctgctc cgggttcgga aaaagctcta aatccttgat
     3421 gacttggcct ttgaccggaa tgcaaaacaa aaagaagagc atggtcatca agaaaaagac
     3481 caaagcgttg ggcatggtgg tgaaaacctc cagaaacaga gcaaaaagag cgattaaact
     3541 gaatacgtac attcttgcgg aagccgcttt ccggtaggcc ggcagacgtt ctttcaacgc
     3601 tttttcgagg cgtattgccc tggttttgtg gtgaaaccaa cgcaggctaa aaggcacgcc
     3661 gatcaaaaac aataggatgc aaaagctttg aaaaggaagg gaaagctctt gcgacaacag
     3721 gatagactgt agtggaaaca aataagccaa caggcctaaa cacaggagta agccaacgct
     3781 gagaaaaaat tgtaagcgca gttttttcag cacaagatgg agatcagctt ccatatcagg
     3841 aggttttaaa aggaattcga ttgagtatgg aacgtcccag cgtgatttca tcggcatatt
     3901 ccaattcgtc acccaccgag attccccggg ctataacagt gattttcagg ggatacgtac
     3961 tgatttttcg aaaaatataa aaggaggtgg catcgccttc ggcggtagcc gaaagggcca
     4021 gaatgacttc ttcaacccgg ttttgtgcga gcctttccac caatgaattg atttcaagat
     4081 cggaaggccc tataccgtcc atgggagata tcaggccgcc cagcacatgg taaaggccgt
     4141 tgtattgcct ggtctgttct atggccatta cttcgcgcac attttctacc acgcaaatga
     4201 tggaggtatc tcgtctggga ttgctgcaaa tcaggcattc ttcggtgtcg gaaatattgt
     4261 ggcaggtctt gcaataggcg atttcatttt tcaggcgtat cagggtgtta ccgaagtgct
     4321 ccacatcctc tttgttttga cgcaaaagat gcaacaccag acgcaaggcg gttttcctgc
     4381 ctataccggg caagccggaa aaagcctgca cggcttcctc caataaaacg gaagggtaga
     4441 tttgatccat tataaatcga acactgaggg ggcaaaagta cgttcaaatc cccaatttta
     4501 aaaataatcc cgtatttttg cagcctgttc aaaagtttat gaatgaattt cttgtactgg
     4561 gactgttatt ggcttatttt ggattgctct tgctgatatc cgtcttatcc aaaggatccg
     4621 accaaaacaa caatgccttt tttctgggag aacgcaagtc gccctggccg gtagtggcca
     4681 taggtatgat aggggcttcg ctttcgggag tgacttttgt ttcggttccg ggcatggtac
     4741 gcgaaaacag ctttttgtac atgcagaccg tgatgggttt tttcgtgggc tatgttttga
     4801 ttgccaatgt cttgctgccg gtttattatc gcttaaattc tcccagtatt tatgtctatc
     4861 tgaaagagcg cttcggcaag tattctt
//
LOCUS       DAOSGL010000162         4866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15511_length_4866_cov_3.529827, whole genome shotgun sequence.
ACCESSION   DAOSGL010000162 DAOSGL010000000
VERSION     DAOSGL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15511_length_4866_cov_3.529827"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKY68_05900"
     CDS             complement(<1..324)
                     /locus_tag="PKY68_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOH24604.1"
                     /translation="MDERIEKLTLMASQLRRDVVNAVYYAKDGHPGPCMSAADLMAVL
                     YFDLLRLNPEDPSWIDRDRFILSKGHACPLWYAALARRGYFPPVELKTLRVLGSRLQG
                     HPDLKK"
     gene            complement(466..1131)
                     /locus_tag="PKY68_05905"
     CDS             complement(466..1131)
                     /locus_tag="PKY68_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acyltransferase"
                     /protein_id="HOH24605.1"
                     /translation="MSKKILIIGGEGNGGVVAACIEDMRLRHRILEWEVAGFINDYEQ
                     SVSGYPVLGGTGDVQDILRRHSDYYLMWAIHTIGRNYLIEKVFNKANIPAERLATIVH
                     PSAFVAPTAHLEPGVFVMSNCYVGPSAHIGLCCMLMANSLVAHDTRVGPLCHFSAGSI
                     TGSYNTIGKCSSIAYGTVVLEKKNIGNFAVAGASSLVTKDIPDGEIHIGIPARFMKKV
                     MEA"
     gene            complement(1292..2770)
                     /locus_tag="PKY68_05910"
     CDS             complement(1292..2770)
                     /locus_tag="PKY68_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH24606.1"
                     /translation="MIHKLADRLRGRNKPAKEGYRTAFKATSLFGGVQVVTVLTGLLK
                     SKLVAVWMGTTGFGILSLYNAAVNLIFSISNLGLQSSAVRDIAAASADKGKTLAIIKA
                     VNRWVLASGLLGALLTVALSPRLSRWLFESDQYVGAFVLLSAAVLLMGLFNQNYAVLQ
                     GLRKLRLMARANIFGALAGFVCSVPVFYFFREQGLAWTVVISALASAVVSLLYVRKLG
                     LVLPKQDWHTSMVLGSDTVKLGIVMAISSIAVFMVQFVVKTYISHRGGLGDVGLYQAG
                     WGLNEQYLGLVFTAMAKDYFPRLSQVAGDRRKVRKSINEQAEIAILILAPMIIAMLVF
                     LPFVVRLLYSGDFDGIVPMTRWLLLGSLLKAGSWAISFVFLAKGHGKTYLFNELGIKF
                     FTLPTYMLGYRLLGLLGVGYAYVLCYGIYFLWVGIVAYRKYRLMYSGVFWRMLAFTTT
                     VLAAYLLMEPLMISGGLHYWLGIPLIVLLSIYSLRELKKRLF"
     gene            complement(2792..3724)
                     /locus_tag="PKY68_05915"
                     /pseudo
     CDS             complement(2792..3724)
                     /locus_tag="PKY68_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
     gene            complement(4057..>4866)
                     /locus_tag="PKY68_05920"
     CDS             complement(4057..>4866)
                     /locus_tag="PKY68_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24607.1"
                     /translation="PGLEVMMVHEEVSALYGVEMHDALTGEIISGRFAGSDVGRGLCA
                     DVDPTSRGHEYWSTASYDVYDGAGTVLSTKRPTVNFRTYWDGDLQCETTERGVITKWQ
                     GRTQNAATLVDMPNKYGAGTNVIKYTPCLQADIFGDWREEQIYYDNATMSHLWIFATP
                     YVSNYRIPTLMHDHHYRMATVTQTAAYNQPPHLSFYLPDYVEYLTGLPGSEAPYDRYS
                     EGDASQGAKQVLSTVYYDLLGRPIAEPERGFYIRENLYSDGSLSRVKCYKP"
BASE COUNT         1270 a         1343 c         1200 g         1053 t
ORIGIN      
        1 ctttttcaaa tccggatgac cttgcaacct ggaacccagc accctgaggg tttttagctc
       61 cacgggaggg aaatagcctc tgcgagccaa agcggcatac cacaaaggac aggcatgccc
      121 cttggacaag ataaaacggt cgcgatctat ccaggaagga tcttcgggat taagccgcaa
      181 taaatcaaaa tacaagacgg ccatcaaatc ggcagccgac atacagggcc cgggatggcc
      241 gtccttggcg taatataccg cattgaccac gtcacgacgc aactgcgacg ccataagggt
      301 cagtttttct atgcgttcat ccataactat gttaaatctt tttgctcaaa agagcattca
      361 gaacccaaat atagggcatt ctccgttctc gacaaaaact ttcgggcctt ctatttcggt
      421 aaaaaatcac aacaaaaagg ccgaaccctt aaaaattacg gccgattagg cttccatcac
      481 ttttttcata aaacgggccg gaattccgat atggatctcg ccgtctggaa tatctttggt
      541 taccaggctg gaagccccgg ctaccgcaaa attgcctatg tttttctttt cgagcaccac
      601 cgtaccatag gctatggagg aacacttacc tatggtgttg taggagccgg taatggaacc
      661 ggccgaaaaa tgacatagag gccccacccg ggtatcgtga gccaccaggg agttggccat
      721 caacatacag cagaggccga tatgagcgga cgggccgacg tagcaattgg acataacaaa
      781 cacccccggc tccaaatgag cagtaggagc cacaaaagcc gaaggatgca ctatggtcgc
      841 caggcgttcc gcagggatat tcgccttgtt aaacaccttc tcgatcaaat aattacgtcc
      901 tatggtgtga atagcccaca tcagataata atcggagtgt cttcgcagaa tatcctgcac
      961 gtcgccggtg cctcccaaca ccggatagcc gctgacgctt tgttcgtagt cgttgataaa
     1021 accggccacc tcccattcga ggatgcgatg gcgcagacgc atatcttcta tacaggcagc
     1081 aaccacaccg ccattgcctt cgccgccgat aatcagtatt tttttactca tatcctaatc
     1141 gttcttatgg tttctcattc tttcttcttg caaaagtatt gtttccaacg gttatacaaa
     1201 agaactttcc gccgagtcct ttggaataat cacctgaatc aaaatgtcct ctgtagatca
     1261 aaatgttctc tgcatgttat aatccattcg actaaaacaa tcgttttttc aattcccgca
     1321 gcgaatagat gctcagtaaa acaatcagcg gaatacccag ccaataatgc aagcctccgg
     1381 aaatcatcag gggttccatc agcagataag ctgccagcac cgtggtggta aatgccagca
     1441 ttctccaaaa taccccggaa tacatcaaac gatatttccg gtatgccact atacccaccc
     1501 aaaggaaata aatgccgtaa caaagcacat aggcatagcc tacgcccagg agtccaagca
     1561 atctgtatcc cagcatgtaa gtgggcaaag tgaagaattt aatgcccaat tcattgaata
     1621 aataggtttt gccgtgtcct ttggccagaa agacaaaaga aatggcccag gaaccggcct
     1681 tgagcagaga gcccagcaag agccagcggg tcataggtac tatgccgtcg aaatcgcccg
     1741 aatagagcag acgcacaaca aaaggcagaa acaccagcat cgctattatc atgggcgcca
     1801 aaatcagaat agcaatctcg gcctgctcat tgatgctttt acgaactttc cgccggtcgc
     1861 cggccacctg cgacagccgg gggaaataat ccttggccat ggccgtaaac accaggccca
     1921 aatattgctc gttcaagccc caaccagcct gataaaggcc cacatcgccc agaccgcccc
     1981 ggtgcgaaat ataggtctta actacaaatt gcaccataaa tacagctata ctgctgatgg
     2041 ccatcacgat acctagttta acggtatcgc tgcccaacac catcgaggta tgccaatctt
     2101 gtttgggtaa gaccaggccc agcttacgaa cgtagagcaa actcaccacc gcagaggcca
     2161 gggccgaaat caccaccgtc caggccaaac cctgttcgcg gaaaaaatag aaaaccggca
     2221 ctgagcagac aaaacccgcc agggcgccga aaatattagc ccgcgccatc agcctgagct
     2281 tcctcagacc ctgcaatacc gcgtaattct gattgaacag gcccatcagc aacacggctg
     2341 ccgagagcaa aacaaaagcc cccacgtatt ggtcggactc aaacaaccaa cgactgagcc
     2401 ggggcgaaag ggcaacggta agcagggcac ccagcaggcc gctggccaac acccagcgat
     2461 tgacggcttt gataatggcg agggtttttc ccttgtcggc cgaagcagcc gcgatatcgc
     2521 gcactgcaga actctgcaag cccagattac tgatcgaaaa gatcaggttg accgccgcgt
     2581 tatacaagct aagtatgcca aaacccgtag tgcccatcca gacagccacc agcttggact
     2641 taagcagacc ggtcaaaaca gtcactacct gcacccctcc aaacaggctc gtagccttga
     2701 aggccgtacg gtaaccctcc ttggccggtt tgttcctgcc ccgcagacga tcggcaagct
     2761 tatgaatcat gggtgcaaat gtaggaattt tctatctgtt tgtcttcagc agttccttca
     2821 gctccgctat cttttgttcg ggatcccagg cgccgccgaa ggtccagagg gccgtgacgt
     2881 cttcggggcg gggcgagcct ttggcctgat ccaggttgtc ggccatccag gcaaaattac
     2941 cgccttcgcg tctgacgttg tgcatataca gcctttgtcc ccaggggcag ggatcgagca
     3001 cctggtcggt ataagcgtag ccgatgggat gatcgatgat tttgtcgctc atggtgcaat
     3061 tgagcagaaa aaactgagcg tcgtgatggt aacgccccag cgtgacgggg cttttagagt
     3121 cgaaatgcga atcggtaatc accaattttt tgtcgtaatc gccacggccg tcgtgccaga
     3181 tcaaggcccg tccatcgccg tagaaactgc aacgcgtggc gtagcaccag cccctcggac
     3241 agagaaaatc cactcccggg caacgcaaat ccaaatcggc gtggtaatac atcccattcc
     3301 cttcgggcgc ccaaagcgcc agggcatcgt tgccgtcggc ccagacgcaa cagtcgatca
     3361 ctatggtgcg ggtagcccgt ccgaaaatgg ccatttgatg agccgtggtg ggctccaccg
     3421 tgctgccgta gttgttgtaa accgtcatgg tgccgataat gcagtcgtcg gccccctcct
     3481 gcagcactat cactccgttg cccaccggct tacccttgta ctcgcgaatg gttcgtttgc
     3541 tcgacagctc ggcaaaaatc aaacgggtgc tgtcgcggtg ctcgcccagc aacactatgt
     3601 tggggcggtc gacgatcact ttttcgttat aagtaccctt ctttatcaaa atcacataag
     3661 gtaccgaaga ttgttcgggc gcggcctcga tagcggcctg aatgctctgc ccgggttcaa
     3721 ccacggcctg gacagcccgg gcagacagag aagccggagc catgcgggaa gtcggagcca
     3781 tgcgggaggc aggaacatta ccggaggcag gagctgtttc tgccctctga gcctgcgcaa
     3841 aaacgctgcc tgtccaaagc agcgctgaga aaagacaaca gcaaatcatt cgtgttttca
     3901 caataataaa atttaagaag aaagaccgaa ggtatatatt tttatttcaa gtaccatact
     3961 caatgtttaa ggcagtttaa actttcttca caggaaaggc cgggagcgat cttgaaagac
     4021 cgcacttaat gcttagggca gtttaaaccc ggtacattaa ggcttatagc attttacgcg
     4081 actcaaactg ccgtcggaat agaggttttc gcggatgtaa aagccacgct caggttcggc
     4141 gatgggccgg cccaagaggt cgtaatagac agtactcagc acctgtttgg cgccctgcga
     4201 ggcatcgcct tcggaatagc gatcgtacgg tgcctccgaa cccggcaatc cggtaaggta
     4261 ctcaacgtaa tcgggcaggt aaaaactcag gtgcgggggc tggttgtaag cggcggtctg
     4321 tgtaacagtg gccatgcggt agtgatggtc gtgcatcagg gtgggtatcc ggtagttgct
     4381 aacgtagggc gtggcaaaaa tccagagatg actcatcgtg gcgttgtcgt aataaatctg
     4441 ctcttcgcgc cagtcgccaa aaatatcggc ttgcaggcag ggtgtgtact taatcacatt
     4501 ggtaccggcc ccgtatttat tgggcatatc caccagggtg gcagcgtttt gcgtacggcc
     4561 ctgccatttg gtaatcactc cgcgctcggt ggtttcgcat tgcaaatcgc cgtcccagta
     4621 ggtgcggaaa ttcaccgtag gacgtttggt cgaaagcacc gtacccgcgc cgtcgtacac
     4681 atcgtaactg gccgtgctcc aatattcgtg acctctcgac gtgggatcca catcggcgca
     4741 aaggccgcgt cccacatcgc tgccggcaaa ccggcccgaa ataatttcgc cggtaagggc
     4801 atcgtgcatt tccaccccgt atagagccga cacttcttcg tgtaccatca tcacctcgag
     4861 gccggg
//
LOCUS       DAOSGL010000163         4856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15564_length_4856_cov_3.793585, whole genome shotgun sequence.
ACCESSION   DAOSGL010000163 DAOSGL010000000
VERSION     DAOSGL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15564_length_4856_cov_3.793585"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PKY68_05925"
     CDS             <1..691
                     /locus_tag="PKY68_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOH24608.1"
                     /translation="QYSSVQEAYEHWRRLICSIGFGSRLGVDLPYEQSGLIPTTDYYN
                     GIYGQRGWRGATIISNGIGQGEVLATPLQMANYCAVICNKGWYITPHIVRQVHGMEAD
                     TSTIKKHVSGIDPEHFEPIIEGMRGAVTSPMGTSKGVSIPGIEVCGKTGTAENPGVDH
                     SIFICFAPKDDPQICMLVFIENGGFGATWAVPMASLMLEKYLKGSIAPGRKWMETRLL
                     NANLMNVELKD"
     gene            675..2141
                     /gene="rodA"
                     /locus_tag="PKY68_05930"
     CDS             675..2141
                     /gene="rodA"
                     /locus_tag="PKY68_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOH24609.1"
                     /translation="MNSKINPISLWKAVDIQTIVIYLFLVIVGWLTVFSASYNYDSAV
                     LFDLNSTAGKQLIWIAVSLVTAFFILLIDAKFYYYLSYNGYIIVMFLLLLTLLIAPEI
                     KGSRSWLVFGPFSIQPAEFAKFITALTLARYLSDYGFNTRHPGSIAILFALLLIPMGL
                     IFLQKESGTALVFLAFLLVFFREGMSGVLLFAGACFLSYFVLGLRFGEQTLWEHTSLG
                     NFSVLLLIALVVSGLLLRYHKKDNKAWLVVLLGNVLPVMAALIWNRFTKWGFDIALLQ
                     LALLGLSVVYALYQSIRHNSRNPALIALFAVLSVAFLYGIDYTFDRVLQPHQQTRILV
                     LLGTMDDPSGVGYNINQAKISIGSGGFWGKGFLNGTQTKLKYVPEQDTDFIFCTIGEE
                     FGFAGSVFLLLVYGYLLVRLIFMADRQHFAFARIYGYCVASLLFVHLLVNVGMVLGLM
                     PVIGIPLPFLSYGGSALLGFTILLFIFLRLDADNMRRH"
     gene            complement(2147..2608)
                     /locus_tag="PKY68_05935"
     CDS             complement(2147..2608)
                     /locus_tag="PKY68_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOH24610.1"
                     /translation="MKPGQLSNIFTILLMLLFSACREDSLYHEFRPLSQWNRTEILCF
                     EDSLAFASGKGEQVEIVIQLRNDNSYPYANLCLNADIFLPDSAYREFIDIKLTKEDGK
                     WAGPGWGSLFTHEVILKSLPITDKFKIELSHAMQDEVLEGIRSVGVCLRLR"
     gene            complement(2605..3813)
                     /gene="ricT"
                     /locus_tag="PKY68_05940"
     CDS             complement(2605..3813)
                     /gene="ricT"
                     /locus_tag="PKY68_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOH24611.1"
                     /translation="MSTKACGCNKIKKLNTFDWLCDLSEAQASTDYVEVQFKNTRKGY
                     YLNSNKIPLQKGDLVAVESSPGHDIGEVSLTGHLVLKQMKKINYRFDQQEVRRIYRKA
                     RPVDLEKFAESKALEHDTMLRSRKIAEELNLNMKIGDVEYQGDGNKAIFYYIADDRVD
                     FRQLIRVLADTFHVRIEMKQIGARQEAGRIGGIGPCGREMCCSSWMTNFVSVSTSSAR
                     YQDLSLNPQKLAGQCSKLKCCLNYETDMYVEAQKGFPAKDIVLETKDASYFQIKTDLL
                     KRQVSYSTDKDSMVNAISIPVERALQIIALNKKGIRVDSLLEQAEEKNRNEFGDVLNS
                     DLQRFDKKRNKNNKKRSNFRSNQGENKTREVRSRTDRNRKNRSRNRNRQDGSNTRNDN
                     RNNQNSPDAK"
     gene            complement(3897..4598)
                     /locus_tag="PKY68_05945"
     CDS             complement(3897..4598)
                     /locus_tag="PKY68_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24612.1"
                     /translation="MLIWLPEKMHTSCANKLLKILEEPQGNTVFLLVSENSEKNLETI
                     YSRAQHIQVPRIADEDMERALQELKAMEPASLKSLVRLAGGSWLEALRLLESDEEDRF
                     FLEQFMRHMRAAYTIAHFPPSKLPEKQKSLQDLRLWSEEMAKIGRERQKNYLSFAQRM
                     IRENFILNTREEKLSYLNPEERSFSSKFFPYINQRNVQAFMDLFALAERHIEQNVQAK
                     MVFFDTAVQSILLFK"
     gene            complement(4598..>4856)
                     /locus_tag="PKY68_05950"
     CDS             complement(4598..>4856)
                     /locus_tag="PKY68_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOH24613.1"
                     /translation="RKFNKLVHPDLHFVFPIYKPDSKKNWICDDFLPQWREVAASKPY
                     FSYNSWMEYIGAGNAQGMIYAEESPRFYANSASRASKQNLK"
BASE COUNT         1051 a         1105 c         1206 g         1494 t
ORIGIN      
        1 ccaatattct tcggtgcagg aagcttacga acactggcgc agattgatct gctccatagg
       61 ctttggcagc cgtctgggag tagacttgcc ctacgaacaa tcgggcttga tacctactac
      121 ggattattat aacggaatat acggtcaaag aggctggaga ggggccacga tcatatccaa
      181 tggcataggc cagggagaag tcctggctac tccgctccaa atggccaatt attgcgccgt
      241 gatatgcaac aaaggctggt atataactcc gcatatcgtg cgtcaggttc atggtatgga
      301 agcagacaca agcaccataa aaaagcatgt ctccggaata gatcccgaac acttcgagcc
      361 catcatcgaa ggaatgcgag gcgcagttac cagccctatg ggaacttcca aaggagtgtc
      421 cattcccggt atagaggtct gcggcaagac aggcacggcc gaaaatcccg gtgtggacca
      481 ctccatattc atatgttttg cacccaaaga cgatccgcag atctgtatgc tggtctttat
      541 cgaaaacgga ggtttcggag ccacctgggc agtgcctatg gccagcctga tgctggaaaa
      601 atatttgaag ggaagtattg ctcccggacg aaaatggatg gaaacccgat tattgaacgc
      661 taaccttatg aacgttgaac tcaaagatta atcccataag cctctggaaa gcagtagata
      721 tacagactat agttatttat ctgtttttag ttatcgtagg ctggctcacc gtttttagcg
      781 ccagttacaa ttacgacagc gcggtcctgt tcgatttgaa cagcaccgcg ggcaagcaat
      841 tgatctggat agctgtttcg ctggtgacgg ctttctttat acttcttatc gacgccaaat
      901 tttattatta cctgtcttac aacggctaca ttattgttat gtttttgctg ttgctcactc
      961 tattgattgc acccgagata aaaggatccc gttcctggct ggtgtttggg ccctttagta
     1021 ttcaaccggc agagtttgcc aaatttataa ccgccctgac ccttgcccgc tatttgtccg
     1081 attacggctt taataccaga catcccggct ccatagctat cttgtttgct cttttactta
     1141 tccctatggg attgatcttt ttacaaaaag aatccggtac tgctttggtg tttttggcct
     1201 tcctgctggt cttttttcgc gaagggatgt cgggcgtgtt gctttttgcc ggtgcctgtt
     1261 tcctgagtta ttttgtgttg gggctcagat ttggtgaaca gaccttgtgg gaacatactt
     1321 ctctgggcaa tttttcggta ctactactta tagccttggt tgtttccggt ttgcttcttc
     1381 gctaccacaa aaaagacaac aaagcctggt tggttgtttt gctgggcaat gttttgccgg
     1441 taatggcagc cttaatctgg aaccgcttta ccaaatgggg ttttgatatt gccctgcttc
     1501 aattggcgct cttgggctta tctgtcgttt acgccttgta ccagagcata cgccacaatt
     1561 ccagaaatcc ggctttgatt gctctttttg ccgtgctctc ggtggctttt ttgtatggta
     1621 tagactatac cttcgaccgt gtgctgcagc cccatcaaca aacacgtatt ctggttttgc
     1681 tgggtaccat ggacgatcct tcgggggtgg gctataacat caatcaggcc aagatttcca
     1741 taggatcggg cggtttttgg ggcaagggtt tcctgaatgg aacgcagacc aaattgaaat
     1801 acgtcccgga acaggacacc gactttattt tctgtaccat aggtgaggaa ttcggctttg
     1861 ccggttcggt gtttcttctg cttgtatatg gttatttgct ggtccgactg atttttatgg
     1921 cagaccggca acactttgcc ttcgcgcgaa tttacggcta ttgcgtggcc agtcttctct
     1981 ttgtgcattt gttggtgaat gtggggatgg tgctcggcct gatgcccgta ataggtatcc
     2041 ctttgccttt cttaagttac ggaggctctg ctttactggg atttactatt cttttgttta
     2101 ttttcctgcg gctggatgcc gacaatatga ggcgacatta agcacattac ctgagcctca
     2161 ggcatactcc cacactccgg atgccttcca atacttcgtc ctgcatggcg tggctgagct
     2221 cgattttgaa cttgtcggtt ataggcaggc tttttaggat cacttcatgc gtaaacagcg
     2281 atccccagcc cggacccgcc cattttccgt cttctttggt cagcttgatg tctatgaact
     2341 cacgataagc gctgtccggc aaaaaaatat ccgcgttcag gcaaagattg gcgtaagggt
     2401 agctgttgtc gttgcgcagt tgaatgacga tttctacttg ttcgcctttg ccggaagcaa
     2461 aggcgaggct gtcttcaaaa cacagaattt cggttctgtt ccattgtgag agtggcctga
     2521 actcatggta gaggctatcc tcccggcagg cgctgaacaa gagcataagc aggatggtaa
     2581 agatgttgct tagttggcct ggcttcattt tgcgtccggg ctgttttgat tgtttctgtt
     2641 gtcgttacgg gtattgctgc cgtcttgcct gttccggttg cgggaacggt ttttcctgtt
     2701 cctgtcggtc ctgctgcgga cttctcttgt tttgttttcg ccttgattgc ttctgaaatt
     2761 ggaacgtttt ttgttgtttt tgtttctttt tttatcgaag cgttgcagat cgctgttgag
     2821 gacatcgcca aattcgttcc ggtttttttc ttcggcctgt tccagcagtg agtccacacg
     2881 gatgcctttt ttattcagcg caataatctg cagggccctc tccaccggta tgctgatggc
     2941 gtttaccatg gaatctttgt cggtggagta actgacctga cgttttaaga ggtcggtctt
     3001 gatctgaaaa tagctggcgt ctttggtttc gagcactatg tctttggccg ggaaaccttt
     3061 ttgagcctct acatacatat ctgtttcgta gttgaggcaa catttgagtt tgctgcattg
     3121 tccggccaat ttttgagggt tcaggctcag gtcctggtag cgggcggagc tggtagagac
     3181 ggaaacaaag ttggtcatcc aggatgagca gcacatttcc cttccgcagg ggcctatgcc
     3241 tcctatacgg ccggcctcct gacgggctcc gatctgtttc atttctatac gcacatggaa
     3301 ggtgtcggcc agtaccctta tcagttgcct aaagtccacc cggtcgtcgg cgatatagta
     3361 aaaaatggct ttgtttccgt cgccctggta ttctacgtcg cctattttca tgttgagatt
     3421 gagctcttcg gctattttgc gggaacgcag catggtgtcg tgttcaaggg ctttggattc
     3481 ggcgaatttt tcgaggtcta cgggacgggc tttgcggtaa atccggcgga cttcctgctg
     3541 atcaaagcga taattgattt ttttcatctg cttgagcacc agatggccgg taaggctcac
     3601 ttcgccgatg tcgtgacccg gtgaagattc caccgccacc aaatcgcctt tttgcagggg
     3661 gattttgttg ctgttcaggt aatatccctt gcgggtattt ttgaattgca cctcgacgta
     3721 atccgtcgaa gcctgagctt cggacaaatc gcagagccaa tcgaaagtgt tcagtttttt
     3781 tattttgtta caaccgcaag ccttggtaga catcaagaag ccgttgtttt gtagtttata
     3841 ggtcataaaa agcttattta agctacaaaa gtaatttttt ttagcttcta acgcaattat
     3901 ttaaacaaga ggatgctttg cacggcggta tcgaaaaaca ccattttggc ctgtacgttc
     3961 tgttcgatat gccgttcggc cagagcaaag agatccataa aagcctgaac gttgcgctgg
     4021 ttgatgtagg gaaaaaactt ggaagaaaaa gatctttctt cgggattgag gtagctgagt
     4081 ttttcttcgc gggtgttgag gatgaaattt tcgcgaatca tgcgttgggc aaagctcagg
     4141 taattctttt gcctttccct gcctattttg gccatctctt ccgaccaaag cctgaggtct
     4201 tgcaggctct tttgcttttc gggcaatttg gaaggaggaa aatgggcaat ggtataagct
     4261 gcccgcatat ggcgcatgaa ttgttccagg aagaaacggt cctcttcgtc cgattccaat
     4321 aaacggagtg cttcgagcca actgcctccc gccagacgaa caagcgattt gaggcttgcc
     4381 ggttccatag ctttgagctc ttgcaaggcc ctttccatat cttcgtcggc aatgcggggc
     4441 acctgaatat gctgagccct cgaataaatg gtttccaggt ttttttcgct gttttccgat
     4501 accaaaagaa atacggtgtt accttgaggt tcctccagta ttttgagcaa tttgttggcg
     4561 caggaagtgt gcattttttc gggcaaccag atcagcatta ttttaaattc tgcttcgatg
     4621 ctcttgaagc tgagtttgcg taaaatctcg gactttcttc ggcgtagatc atgccctggg
     4681 cattgccggc tccgatgtat tccatccaac tgttgtagct aaagtagggt ttacttgcgg
     4741 ccacttctct ccactgcggg agaaagtcgt cgcaaatcca atttttcttg ctgtcgggtt
     4801 tataaatggg aaacacaaag tgcaaatcgg gatgaaccag cttgttgaat ttgcgg
//
LOCUS       DAOSGL010000164         4841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15625_length_4841_cov_4.249478, whole genome shotgun sequence.
ACCESSION   DAOSGL010000164 DAOSGL010000000
VERSION     DAOSGL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15625_length_4841_cov_4.249478"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PKY68_05955"
     CDS             <1..712
                     /locus_tag="PKY68_05955"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOH24614.1"
                     /translation="AWGGKFAFDLDNKLTAKLLEAGAFGPDRLYSDRKNFVLEGGSIE
                     SDGAGSLLTTSECLLSPTRNPGMNRDEIELYLKQAFGLQRVLWIENAYLQGDDTDGHI
                     DMLARFCNPDTIAYVSAGFPRTAGGHEPKQLESLRAMEGELKSFRTLSGQSYRLIPLP
                     LPDVVRYKNEVLPASYANFLIINGAVLLPVYGQEKDQQAVKQLAKAFPDREIIPVNCL
                     ALIKQHGSLHCISMQHPV"
     gene            842..2215
                     /locus_tag="PKY68_05960"
     CDS             842..2215
                     /locus_tag="PKY68_05960"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH24615.1"
                     /translation="MISSQRYLLFFLLMSLLLACTNTTGQYDLTYPVVRMDFSDELVL
                     EGSVEATNSTFIKCPDVYNSKIIYLVEDGAWVEAGDTVCILENQQLVNQLETVKRLCE
                     LYQAEYNKGVADLELDYALLQAQVKNNETQMAISSLDSLQLKYYTENQRKIAEIRLEM
                     ARIESRKLKKKLSALETIQRSELRKMELQITQSQTLLESTRASLDELILKAPKAGMAI
                     RGDSDISDGKIQEGEEVYPQTPLVIIPDSGQVRIKIQATEAAYKRLALGQKVEYTFDA
                     MPGNFAYGSIVMKSPRGYTMSRNSRVKFFDVLASVDSGLQTPTAGLSANCRVFLNYLP
                     DTVAVPIVSVFQEDSINVVYIKKGSGFIRQEVSVALQSSKEAVIACGLRGGEDIAMLQ
                     PLRSKVKDEVFLDYEQRNRFKTAKNDSLPADFLEVPQHIVPRRDSPEQMEEEPTFQFQ
                     DHIIIVR"
     gene            2225..3457
                     /locus_tag="PKY68_05965"
     CDS             2225..3457
                     /locus_tag="PKY68_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOH24616.1"
                     /translation="MKKSILLLLPIGLLCAACGKEKKQEIPLTQVRQETFYIDLYEEG
                     DIQATQSKVISSPEISWRYGDLKITYVVDDGQEVEAGDTVVIFDPSEVRKAIVDAESR
                     LEIHYAELEKMQAQQESDMEEMMADFEVTELSLEISRLEYESAEYEAVIKKKEIELNL
                     ERATIALEQAKSQIENRKKIQKEELTQKKLIISQAKSELEDAYNTLNLLFVTSPSPGI
                     AIVSQNNTTGLKFQEGDQVWKGQPMIELPNLNELKAAIKINEVDIAKITKGQRVIIRP
                     DAFSDSLYFGHIQTVANLAVNKERNSRIKVFPVDVLINSYPTDLMPGLTVSCRIIIDE
                     IPDAVYIPLNALFKEGIQDIVYVKTATGFRIQEVKTGQSNADYIIIEEGLKAGENIAM
                     ANPFLVAEANKSSKKQAQ"
     gene            3454..4662
                     /locus_tag="PKY68_05970"
     CDS             3454..4662
                     /locus_tag="PKY68_05970"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH24617.1"
                     /translation="MKRTYFSLLLFGLSAGLFLLPGCKQTAQESNLIKRGDFKQTITE
                     TGELFTVDNRSIVMPRYGRYWYNMKIIGLAEHGSKVKAGDSIVQFDPTEVKKFIMDRE
                     TRLENEQANLEKLLVQQQNNLKNLQSTLRGEEASFNLRKLAMEYTKFESERVQEIKRL
                     QFRQAEINFEKVKRRIELTKVMAASDLKIQKIKVAQIENEIKMAQDALPQLTMRAPIS
                     GIFQVARKRRSRDNIAVGDDVRFGTMVGSVPDLTWMKVQTTVNEVDRAKITQGQPVIV
                     RLDALPQVAFDAQVSFISVLCRPYDNNDRRKVFDIEVKLLESDERLKPGMTVSCEFIA
                     NDLKDVLYVPNTCLLKEGGRYWIFMPKATGFDKVAVEFHTRNNTHSVISGNIAEGQQV
                     IPVAGIIDQE"
     gene            4669..4809
                     /locus_tag="PKY68_05975"
                     /pseudo
     CDS             4669..4809
                     /locus_tag="PKY68_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT         1405 a         1056 c         1190 g         1190 t
ORIGIN      
        1 tgcatggggt ggaaaatttg cttttgattt ggacaacaaa ctgactgcca aacttctgga
       61 ggccggagcc tttggtccgg accgcctcta ctccgaccgg aagaactttg tgctcgaagg
      121 cggcagtata gaatcggatg gagccggcag cttgctaacc acctcagaat gcctgttgtc
      181 tcccacgcga aatccgggaa tgaacagaga tgaaattgag ttgtatttaa aacaagcctt
      241 cggtctgcaa cgggtgcttt ggatagaaaa cgcctatttg cagggagacg ataccgacgg
      301 gcatatcgat atgctggcgc gtttttgcaa cccggatacc atcgcttatg tttcggccgg
      361 ttttcctcgg acagccgggg ggcatgaacc taagcaactt gagtccctga gagccatgga
      421 gggagaatta aaatcgttca ggacactttc gggacagtcg taccgcttga ttcccctgcc
      481 tttgcccgat gtcgttcggt acaaaaacga agtattgccg gccagttatg ccaatttttt
      541 aatcattaac ggagccgttt tgttgcccgt gtacggacaa gaaaaagatc aacaggctgt
      601 aaaacagctt gctaaagctt ttcccgatcg ggaaattatt ccggttaatt gcctggcgtt
      661 gatcaaacag cacggctcct tgcattgcat aagtatgcaa catccggtat aagaaaggcg
      721 aagatatgta taaacatttg agatatttat atctttttaa gactattctt aggagacgtt
      781 ttaacttaat gcaaggctgt tatgtaaaat tcatttagct ttgcctgtcc atatttcacc
      841 aatgatttcg tcgcaacgat acttgctgtt tttcctattg atgagcctgt tgttggcctg
      901 cacaaataca acagggcaat acgatttgac ttatcccgtg gttcgcatgg atttttcgga
      961 cgaattggtg cttgagggta gtgtcgaagc aacgaattcc acctttatca aatgcccgga
     1021 tgtctacaac tctaagataa tttaccttgt cgaagacggg gcctgggttg aagccggcga
     1081 cacggtttgc atactggaaa atcagcaatt ggtcaatcaa ctggagacgg ttaaacggct
     1141 ttgcgaactg tatcaggcag aatacaacaa aggggtggcc gatttggagt tggattacgc
     1201 cttattgcag gctcaggtca aaaacaacga aacgcaaatg gccattagta gtctggattc
     1261 cctgcaattg aagtattaca ctgaaaatca gcgaaaaata gcagaaattc gtttggaaat
     1321 ggccaggata gagtcccgta aactcaagaa gaaactaagt gccttagaaa ccatccagcg
     1381 ctcggaactt cgtaagatgg agttgcaaat cactcagagc caaacgctgc tcgaatcgac
     1441 ccgggcttcg ctggacgagc tgattttgaa ggctccgaaa gccggcatgg ccataagggg
     1501 cgattccgat attagtgacg gcaaaataca ggaaggagaa gaagtgtatc cgcaaacgcc
     1561 tttagtgatt attcccgatt ccggccaggt aaggataaaa atacaagcca ctgaggcagc
     1621 ttataagcgc ttggctcttg ggcagaaagt agaatacact tttgacgcta tgccggggaa
     1681 ttttgcttac ggatccatag taatgaaatc ccccagaggt tataccatga gccgcaattc
     1741 cagggtgaag ttttttgatg tgctggcttc ggttgacagc ggtctgcaaa cgcccacagc
     1801 cggtttatcg gccaactgca gggtcttttt gaattatttg cccgataccg tggctgttcc
     1861 cattgtttcg gtttttcagg aagacagcat caacgtggta tatataaaaa aaggttccgg
     1921 ttttatccga caggaagtgt cggtagccct gcaatcgtcc aaagaagccg taatagcctg
     1981 cggtctgcgg ggtggagagg acattgctat gttgcaacct ctcaggtcga aggtcaaaga
     2041 cgaagttttc ctggattatg aacagcgtaa tcgctttaaa accgccaaaa acgacagttt
     2101 gcctgcggac tttttggagg tgccgcaaca catcgtacca cgacgggatt ctcccgaaca
     2161 gatggaagag gagcctacgt ttcagtttca agatcatatt attattgtaa gatagtccta
     2221 caggatgaaa aagagtattt tattattatt accaatcggt cttttatgtg ccgcctgcgg
     2281 caaagaaaag aaacaggaaa ttccgttgac ccaggtcaga caggagacat tctacataga
     2341 tttgtacgaa gaaggagata ttcaggctac gcagtccaag gtgatttctt cccccgaaat
     2401 ttcgtggcgc tacggcgacc ttaagattac ttacgtagtg gacgacggcc aggaagtgga
     2461 agccggcgat accgtggtta tttttgatcc ctccgaagtg cgcaaggcca tcgtagacgc
     2521 cgagtcgcgc ctggagattc attatgcaga gctcgaaaaa atgcaggctc aacaggaatc
     2581 tgatatggaa gaaatgatgg ccgatttcga agtgacggag ttgtcgctcg aaatttcccg
     2641 tctcgaatac gaatcggccg agtacgaagc cgtaatcaag aaaaaagaaa tcgaactcaa
     2701 tctcgaaaga gccaccattg ctttggaaca agcaaaaagt cagatagaaa atcgcaaaaa
     2761 aattcagaaa gaagagctaa ctcaaaaaaa actcatcata agccaggcaa agtctgaact
     2821 ggaagatgcc tacaatacct tgaacttatt gtttgttact tccccttcgc cgggcattgc
     2881 cattgtaagt caaaacaaca ccacaggact taaatttcag gagggcgatc aggtatggaa
     2941 aggtcagcct atgatagaat tacctaatct gaacgaatta aaagcggcga taaaaatcaa
     3001 cgaagtggac attgctaaaa tcaccaaggg tcaacgagtg attatacgtc ccgatgcttt
     3061 ttcggattcc ttgtattttg gccacattca aaccgtagcc aatctggcgg ttaacaaaga
     3121 acgcaactcc agaatcaagg ttttccccgt ggatgtgctt atcaacagtt atccaaccga
     3181 tttgatgccg ggccttaccg tgagttgccg cattatcatc gatgaaattc ccgatgccgt
     3241 ttatattcct ttgaatgctc tttttaagga aggtatccaa gacattgtat atgtgaaaac
     3301 agctaccggg tttaggattc aggaagtcaa aacggggcag tccaatgcgg attacattat
     3361 tatcgaagag gggctcaaag ccggagaaaa cattgcgatg gccaatcctt tcctggtggc
     3421 cgaagccaat aaatcttcca aaaaacaggc acaatgaaac gtacatattt ttccctgctg
     3481 ttgtttggac tgagtgccgg tttattcctg ttgccgggtt gcaagcaaac ggcacaagag
     3541 agtaacctga taaaacgggg cgatttcaaa caaaccataa ccgaaaccgg cgaattattt
     3601 actgtcgaca accggtctat agtgatgccc cgctacggca ggtattggta caacatgaaa
     3661 attatcggct tagcggagca cggatcaaag gtaaaagccg gcgattccat cgttcagttc
     3721 gatcctaccg aagtgaaaaa attcatcatg gatcgcgaga cccgcctgga aaacgaacag
     3781 gctaatttgg aaaaattgct ggttcagcaa caaaacaacc tgaaaaatct gcagtccacc
     3841 ctgcgtggag aggaagcctc cttcaacctg cgcaagcttg ccatggaata cactaagttt
     3901 gagtcggaac gggtgcagga aattaaaaga ttgcaatttc gtcaggccga aatcaatttc
     3961 gaaaaagtaa aaagacggat tgaactaacc aaggtaatgg cagccagcga tcttaaaata
     4021 caaaagatca aagtggcgca aatagaaaat gaaatcaaaa tggctcagga cgctctgccg
     4081 caactcacga tgcgtgcgcc catttcgggc atattccagg tggcccgcaa acggcgttcg
     4141 cgcgacaata tagccgttgg agacgatgtg cgtttcggca cgatggtggg cagcgtcccc
     4201 gatctgacct ggatgaaggt tcagacaacg gtcaacgaag tagaccgggc taaaatcaca
     4261 cagggacagc ctgttattgt gcgcctggac gccttgccgc aagtggcttt cgatgctcag
     4321 gtgtctttca tcagtgtgtt gtgtcgtcct tacgacaaca acgacagaag aaaggtgttc
     4381 gatatagaag taaaacttct cgaatcggac gaaaggctaa aacccggaat gaccgtcagc
     4441 tgcgaattta tagccaacga tctgaaggac gtgttatatg tacctaatac ttgtttgttg
     4501 aaggagggcg gacgttactg gatatttatg cccaaagcga ccggatttga taaggtggcc
     4561 gtagaatttc atacccgcaa caatacgcat tccgttatca gcggcaatat tgccgaaggc
     4621 cagcaggtga ttcctgttgc cggaattata gatcaagaat aaaatactat gcaattcgaa
     4681 gaaatagtgc gtgtggctct gcacgccttg atggatcata aattccgttc tttcctcacc
     4741 atgttgggca tcatctttgg ggtggcatcg gttattgcca tgctttccat aggtgaaggt
     4801 gccaagaggg aagccattgc caagtagcag gacctggggg t
//
LOCUS       DAOSGL010000165         4795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15843_length_4795_cov_3.448945, whole genome shotgun sequence.
ACCESSION   DAOSGL010000165 DAOSGL010000000
VERSION     DAOSGL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15843_length_4795_cov_3.448945"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PKY68_05980"
     CDS             complement(<1..1314)
                     /locus_tag="PKY68_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOH24618.1"
                     /translation="MTDFFKHFIQGFELPLQNPVLILSLLLFIILLSPILLRKINIPG
                     IVGLIIAGVIIGPYGFNIIESSSSIDLFSTIGLLYIMFIAGLELDLNEFKSNRNKSLL
                     FGIFTFTFPLAIGYPVCHYLLGYDTNASLLISSMFATHTLVSYPIVSKLGVSKNQAVA
                     ITVGGTILTDTAVLTLLAVIVGKSQGGLDSEFWIRLGVSFAIFLAIMFFLIPRIAKWF
                     FQKLESEKHSHYIFVLSIVFFAAFLAEAAGIEPIIGAFFAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDVSVLLKGPTAIIVIVTLSVVALFGKWVAAFFTQLIFR
                     YSKAQRQLIFGLSSSHAAATLAVILVGYKANILDENILNGTIILILITCLVSSFVTEK
                     AAKKIVVESENDSSDLVKSNGIRSEHILLPIANFSNIEKLLEFAVL"
     gene            complement(1407..4298)
                     /locus_tag="PKY68_05985"
     CDS             complement(1407..4298)
                     /locus_tag="PKY68_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH24619.1"
                     /translation="MKRLIIASLLLLSLACSRYDRYEMDLAAEWKFQIDEDDLGMDQK
                     WFDTRLSGRMALPGSMMEAGLGKELTLQTQWTASIYDSSWFFNPDLEPYRRPGQLKFP
                     FWLTPPVYYVGPAWYQRELRIPRDWEGRQLILRLERPHWESQVWVNSEPAGLQNSLSV
                     PHEYDLTSLLRPGKNRLTLRIDNRVKDIKPGPDSHSISDHTQGNWNGIAGEIKLFARP
                     KIMLSDVQVYPDPLSGRVRLVCSIDNRSGEPCEGELLAEARLFNVEQKAGSSKAGQKA
                     GSQRASFPVQLGTGEQSLEFVMEMGEERQLWEEFHPALYKLSLELSCGKVDADRFETQ
                     FGFRSFEADGTRFLINGRPVFLRGTVECAAHPLTGYAPMDKAYWRELYQKAMAYGVNH
                     FRFHSWCPPRAAFEAADELGIYLQPEGPFWTNHGTAVGNGWPIDQYIREECDRILKAY
                     GNHPSFVLFAYGNEPAGRHQITFLNGLVDYWKEQDPRRLYTHAAIGRSWPLAPSNEFI
                     IRSESRGLPWDRRPNSEFDFSDKIAPYNVPYLAHEMGQYCVFPDFGEIPKYTGVYRAK
                     NFEMFKAELERKNMGPQARDFFMASGKFQAICYKNEIEASLRTPGNGGTQLLSLNDFP
                     GQGSALVGVTDVFWEPKDYIRPEEFKRFFSPTVPIARFPKFVFCNDEQLSVTAEVAHF
                     GPEVLKNRLPVWKVSDDNDNIVAEGRLPETDIAFGNVALGTISLPLDFVREAGQYKLA
                     LSLDNCSNQWDFWVYPALLPEPDTTGIFITRDYKQAAEQAQQGAKVLLLADGKVEKGK
                     EVVQYFRPVFWNTSWFQMRPPHTLGILTHPEHPALAAFPTEYHSNLQWWELLHNQQVM
                     NLDDFPADFIPIVQPIDTWFINRKLGLIFEAKLGRGCLIVCSADISSAPDRRIVARQM
                     RYSLLNYMNSDQFAPSFEISQGQLSTLFEQGEGDNYDVRTSDSPMDLKP"
     gene            complement(4330..>4795)
                     /locus_tag="PKY68_05990"
     CDS             complement(4330..>4795)
                     /locus_tag="PKY68_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24620.1"
                     /translation="KLIAARGETGKAKSPLKSMSAWSIGLPAVEPELELIRADDRHFV
                     LRAPKLQLDEISDVFVRFDYRGDRGLCMMKGELRSDNLYTSGPWTLGLKRYLPDLKEN
                     EMYFYFIPMRNNAPFLRWLDPEVVPDFGERKEFLEIKEPEIISEYRVKIELQ"
BASE COUNT         1288 a         1209 c         1136 g         1162 t
ORIGIN      
        1 aagaacggca aattcaagaa gtttttctat gttcgaaaaa tttgcaatag gcaaaagaat
       61 atgttcactc cggataccat tcgatttcac aagatcgctc gaatcatttt cagactccac
      121 aacaatcttt tttgccgctt tttctgtaac aaacgacgat accaaacagg ttatcaaaat
      181 aagtataatg gttccattga gtatattctc gtcgagaata ttggctttgt agcccactaa
      241 aattacggca agagttgcgg cggcatggga actgctgaga ccaaaaataa gttgtctctg
      301 agcttttgaa tacctgaaaa taagttgcgt aaaaaaagcg gcaacccatt taccgaacaa
      361 agctacgacg gacaatgtta caataacaat aatagctgtc ggacctttta ataaaacgct
      421 tacatccacc aacattccaa cgcttattag gaaaaacgga atgaataacg aatttcctat
      481 aaactcaata cggttcatca atgcagaaga atggggaata agtttattca atgccaatcc
      541 ggcaaaaaac gctccaataa taggttctat ccctgcagcc tcggctaaga atgccgcaaa
      601 aaacacaatg gacaaaacaa agatgtagtg cgaatgtttt tcactctcca gtttttgaaa
      661 aaaccatttt gcaattcttg gaatgaggaa aaacatgatt gccaaaaaaa tcgcaaaaga
      721 aacacccagt ctaatccaaa actcagagtc gaggccaccc tgacttttac cgacaattac
      781 agccaaaagc gtaagtacgg cagtatcggt caatatggtt ccgccaacag ttatggcaac
      841 cgcctgattt tttgaaacgc ctaatttgct tacaatcgga tacgatacca gtgtatgtgt
      901 ggcaaacatg ctcgatatga gcaaacttgc attagtgtcg tatccgagca aataatgaca
      961 taccgggtat ccgatagcta aggggaaagt aaatgtaaaa attccgaaca gcaggctctt
     1021 attcctgttt gatttaaact cgttcaaatc gagttcaagt ccggcaataa acatgatata
     1081 gagaagtcct atggtcgaaa aaaggtcgat agaagaactg ctctcaatga tgttaaatcc
     1141 gtagggccca attattactc cggcaatgat caagcctaca attccgggaa tgtttatttt
     1201 tctgagtaaa atgggcgaca ataaaataat gaataagagc aacgagagta tcaatacagg
     1261 attctgtagt ggcaattcaa atccttgtat gaagtgttta aaaaaatccg tcatttactg
     1321 tctctttatt cgtttattta tatcggccca aaattactct ttttatttag agcggcggca
     1381 aaaaattaaa ctaatttacg gtatactcaa ggcttcaaat ccatcgggct gtcggaggtg
     1441 cgcacatcgt agttatcgcc ttcaccctgc tcgaaaagcg tcgagagttg tccttgcgaa
     1501 atctcgaagg agggtgcaaa ttggtcgctg ttcatgtaat taagcaggct gtaacgcatt
     1561 tgacgggcta caatgcgcct atcgggagcg gatgaaatat cggcgctgca cacaatcagg
     1621 cacccgcgac ccagcttggc ctcaaaaatc aggcccaact tgcggttgat aaaccaggta
     1681 tcgatgggct ggacgatggg aatgaaatcg gccggaaaat cgtcgaggtt catcacctgc
     1741 tgattgtgca gcaattccca ccattgcagg ttactgtgat actcggtggg gaaagctgcc
     1801 agggccggat gctccggatg ggtcaatatg cccagggtgt gcggaggacg catctgaaac
     1861 caggaggtat tccaaaacac cggtcggaaa tactgcacca cctctttgcc tttttccact
     1921 ttgccgtcgg ccagcaacaa aaccttagcc ccttgctgtg cttgctctgc ggcctgttta
     1981 taatcacggg taatgaaaat accggtggtg tcgggttcgg gcagcaaagc cggatatacc
     2041 caaaaatccc attgattgga gcagttatcc agcgacaggg caagcttgta ttgcccggcc
     2101 tcacggacaa aatcgagcgg caacgaaata gtacccagcg ccacgttgcc aaaagcaata
     2161 tctgtttccg gcagccgacc ttcggctacg atattgtcat tgtcgtcgct gactttccac
     2221 acgggaaggc ggtttttaag tacttccggt ccaaaatgag ccacttcggc cgttaccgat
     2281 aattgctcgt cgttgcagaa aacaaacttc ggaaaacgtg ctatgggcac cgtgggcgaa
     2341 aaaaagcgct tgaattcttc gggcctaata taatccttgg gctcccaaaa cacgtcggtc
     2401 acacccacca gcgcactgcc ctgacccgga aaatcgttca acgacagcag ttgagtgccg
     2461 ccattgccgg gagttcggag cgaagcttca atttcgttct tatagcagat ggcctgaaac
     2521 ttgcctgagg ccatgaaaaa atcccgggcc tgagggccca tattctttct ttccaattcc
     2581 gctttgaaca tctcaaagtt tttggcccgg taaaccccag tgtatttggg tatttcgccg
     2641 aaatcgggaa aaacacaata ctgtcccatc tcgtgagcta agtaaggcac gttgtagggt
     2701 gcgatcttgt cggaaaaatc aaactcgctg ttggggcgcc tgtcccaggg caggcctcgc
     2761 gattcggaac ggatgataaa ctcgttcgaa ggcgccagag gccagcttcg tccgatggcg
     2821 gcgtgggtat agagccggcg cggatcctgc tctttccagt aatccaccaa tccgttgagg
     2881 aaagtgatct gatggcggcc tgccggctcg ttgccgtaag caaatagcac aaaagaagga
     2941 tgattgccgt aggctttcaa aatacggtcg cattcctcgc ggatgtattg atcgatcggc
     3001 cagccgttgc ccacagccgt gccgtgattg gtccagaaag gcccttcggg ctgcagatag
     3061 atgcccagct cgtcggcggc ctcgaaagca gcccggggcg gacaccagga atgaaaacga
     3121 aaatgattga ccccataggc cattgccttt tggtaaagtt cacgccaata agccttgtcc
     3181 atgggagcgt agcccgtcag cggatgggcg gcacattcca ccgtaccgcg caaaaaaacc
     3241 ggacgtccgt ttataagaaa gcgcgtgccg tcggcttcaa aactgcgaaa gccaaactgt
     3301 gtttcgaaac ggtctgcatc aacctttccg caactgagct caagagacaa cttgtataaa
     3361 gccggatgaa actcctccca gagctgtcgc tcctcgccca tttccatcac aaactccaga
     3421 ctttgttcgc ctgtaccgag ctgaaccgga aaacttgccc tctgcgaccc ggctttttgt
     3481 cctgccttag aggaaccggc tttttgctcc acattaaaca gacgggcctc agccagcaat
     3541 tcgccctcgc agggctctcc gctccggttg tcgatgctgc aaaccaggcg gacacgtccg
     3601 ctgaggggat cgggataaac ctgaacgtca ctgagcataa tttttggtct ggcaaacagc
     3661 ttaatctctc cggcaatgcc gttccagttg ccctgagtgt gatcgctgat actgtgcgag
     3721 tcgggacccg gtttgatgtc ttttacccgg ttgtctatcc tcagggtcag acggttcttc
     3781 cccggcctca acaagcttgt cagatcgtat tcatgaggca ccgatagcga attttgcagg
     3841 ccggcaggct cggagtttac ccatacctgc gactcccagt gggggcgttc cagcctgagg
     3901 ataagttgcc ggccctccca atcacgagga atcctcagtt cgcgctgata ccaagcgggc
     3961 cccacgtaat acaccggagg cgtcaaccaa aacggaaact tcaactgccc gggccgacga
     4021 taaggctcca aatcgggatt aaaaaaccaa gaactgtcgt aaatactggc tgtccattgg
     4081 gtttgcagcg ttagttcctt tcccagaccg gcttccatca tggagccggg caaagccatg
     4141 cgaccggaca gccgggtatc aaaccatttt tggtccatgc ccaggtcgtc ttcgtctatt
     4201 tgaaacttcc actctgccgc cagatccatc tcgtaacgat cgtaccggct gcaagcgagg
     4261 cttaaaagaa gtagtgatgc tattatcagg cgtttcatat ctattggttt tttcggtgat
     4321 ttagttgatt tactgcagtt ctattttcac tctgtactca ctgatgatct ccggctcctt
     4381 gatctccaga aattctttgc gctccccaaa atcgggtaca acttccggat cgagccagcg
     4441 cagaaacggg gcgttgtttc tcataggaat gaagtaaaaa tacatctcat tctctttcaa
     4501 atcgggcaga tagcgtttga gtcccaaggt ccaggggccg ctggtgtaga gattgtcgct
     4561 gcgcagttcc cccttcatca tgcagaggcc gcggtcgccc cggtaatcga aacggacaaa
     4621 tacatcgctg atttcatcca actgaagctt gggggcccgc aatacaaaat ggcggtcgtc
     4681 tgcgcgaatc agttccaact caggctcgac ggcaggcagg ccgatgctcc aggccgacat
     4741 ggatttcagg ggcgatttcg ctttcccggt ctctcctctc gcggctataa gcttg
//
LOCUS       DAOSGL010000166         4794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15845_length_4794_cov_4.042625, whole genome shotgun sequence.
ACCESSION   DAOSGL010000166 DAOSGL010000000
VERSION     DAOSGL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15845_length_4794_cov_4.042625"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PKY68_05995"
     CDS             <1..675
                     /locus_tag="PKY68_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HOH24621.1"
                     /translation="FRPSDDATTFQFLIPSNFFAVTLLRKAAEILTEVNNETALAQEC
                     LSLADEVEASLREYAIYNHPKYGKIYAYEVDGFGNQLLMDDANVPSLLAMAYLGDVDI
                     DDPIYQNTRSFVWSKDNPYFFSGKAGEGIGGPHIGYDMVWPMSIMMKAFTATDDTEIK
                     KCIEMLMATDAGTGFIHESFHKDDPAHFTREWFAWQNTLFGELILKLIDEGKLDLLNS
                     ITVNNE"
     gene            865..3621
                     /locus_tag="PKY68_06000"
     CDS             865..3621
                     /locus_tag="PKY68_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24622.1"
                     /translation="MNKLKSLLAVFSISAMALFTFTHCTKEEQSELSLDSFEQFVTIS
                     GKVVYSTGVDTTSTDYTIEVMKPAAGRKVFVEVTYASYDPGAAGNKIFETETDEVGEF
                     SIEVPTTPLGINATIRMEEFTDFYSEYVKMENGRPVFKTRLRRYQLQFPALPSSLKTG
                     AFSFPEALTYGSTEIDMEGYDEKITLTGNVQLAYESSYRTGAYKPASSGTVELEIVYD
                     PAGANPLTVVYGAATDANGNYSVTLPLKSYKDGFQIKEVSIKGIGQAAYTHYYEPNKT
                     KSLSGAYATNKLAGFPINFTNITKDIPHSLGQQFLKFTPGHNDGLPQADFAPESWTDD
                     LAGWEKYPGFNETLTVTGTCYMAAETGFAVGSYKTGVQTVKLNVNYGGVLGAKTLFAA
                     TNPDGSFSIDLPAEKSTDVYAVALLAPEEKEFLHYKSAAESIKLSGAYNLYANIKKEA
                     PKWYELGDHYYKFTPDAPQPATWHGDLAGWVKKKDHNLKATVSGKVYLAKESAFATGR
                     YVPAANKVIQIDVGTDSYQGMVSSDGTLSIDVLIENSSDELAVALGTGTINDVKDFEH
                     YTKGGTTNIRTLKGDYTATQLKSASAKWNELGDIYYTFTPDAPPATWQQDLAGWQVRL
                     DHKFSANITGTLRLPVEESFRVGAYRGAAYQIVKLVSNGFTLVGATNAEGNFSIPVPL
                     QYADDEPGAAWHGDMATLKTTSFSHFRKPASGSTEILEGNYTFKLTNLPAGSPWNVLG
                     TRYYDFAPDGAPTNWSNNLPGWVVVPNYSAVIMVKGKVKKAVQKREGVLWIAGWEVDR
                     NRMVTVTVAGTPFDVVTSSNGDIVFVYPANNAPDNLTITITPDNDDTDVPFVHHPDVT
                     KTNYVSIYGKFVSANNIAAKSIARDPSSSLYNVTESAKMLYSPVGSPDGWGSYDWASI
                     LDQE"
     gene            3696..4559
                     /locus_tag="PKY68_06005"
     CDS             3696..4559
                     /locus_tag="PKY68_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24623.1"
                     /translation="MSQAQNVEQKQKPILPKAGDIGLGIDLVPVLRYAGNIFNGTANN
                     ALNIFGGEPALPIGVPAAPNVSISGRYMLTDEFSVRANVGLIGRNLSQQLYVRDDVAF
                     FNNPLSEAKITDTYKQSRSGMILSFGPEYRRGYHRIQGYAGLNLLYGFEAQRDVYLYG
                     NAVTEINQTPSRNNAAFAVPPALTPAVPAYWTRTYVTGKYQDGNTHYVGLEAVVGVEY
                     YIASHLSLGGEVSLYALQAFMSDSYQEQEGFNFLTNKVEQRTELLSPSSREFNYGTDN
                     LGAKLYMMFYF"
BASE COUNT         1384 a         1005 c         1085 g         1320 t
ORIGIN      
        1 ttccgcccat cggatgacgc aacgactttt cagtttttga tcccatcaaa cttctttgcc
       61 gtgactttgc tcagaaaagc agcagagatt ttaacggaag tcaacaacga aactgccctg
      121 gcgcaagaat gtcttagctt agctgatgaa gtggaagcgt cacttagaga atacgctatt
      181 tacaaccacc cgaaatacgg aaaaatatac gcatacgaag tggatggatt cggcaatcag
      241 ttgctgatgg acgatgccaa tgtacccagc ctcctggcta tggcttatct gggtgatgta
      301 gatatcgatg accctattta tcaaaacacc agaagttttg tttggagtaa agacaatccc
      361 tactttttta gcggaaaagc aggcgaagga atcggcggac cgcatatcgg gtacgatatg
      421 gtttggccta tgagtattat gatgaaagca tttaccgcta ccgacgacac tgaaataaaa
      481 aaatgtatcg aaatgcttat ggcaaccgac gccggtaccg gatttataca tgaatcgttt
      541 cacaaagatg accctgctca ttttacgcgg gaatggtttg cctggcaaaa cacgcttttt
      601 ggcgagttga tattgaagtt gattgatgag gggaaattgg atttattaaa tagtattaca
      661 gtaaataacg agtagatgag aaaatctcgg tgtctcctaa ctgtcctatt gctgtctttg
      721 cttttctggc gactcctttg atatatttaa catcgaaatc ctgtgttggt taacaaaatt
      781 tgattatcat tgtagaattc tttattcacg gataattttg atatcttgta taccttattt
      841 aataaccatt tttaatgttg tcgtatgaat aaattaaaat ctttacttgc tgtattctct
      901 atttcggcaa tggctttatt tactttcacc cattgcacta aagaggaaca atcggagttg
      961 agtctcgact catttgagca atttgttacg atttccggta aagtagttta tagcaccgga
     1021 gtggacacca cctctacgga ttataccata gaggtcatga aaccggcggc aggtcgaaaa
     1081 gtctttgtag aagtcaccta cgcaagttac gatccgggtg cggcaggaaa taaaatcttc
     1141 gaaaccgaaa ccgatgaggt tggagaattt tctattgaag ttccgaccac tcctttgggc
     1201 ataaacgcta ccattaggat ggaagaattt acagattttt acagcgaata tgtaaaaatg
     1261 gagaacggga ggcccgtctt caagacgcgt ctcagacgat atcaattgca attccctgct
     1321 ctccccagtt cgttgaagac gggagcattt tctttcccgg aagccttgac ttacggctcg
     1381 acggaaatcg acatggaagg ctacgacgaa aagattactt taacggggaa tgtacagctt
     1441 gcttatgaat cctcataccg taccggagcg tacaaacctg cttcttcggg aacggttgag
     1501 ctggaaatag tgtatgaccc ggcgggtgcg aaccctttga ccgttgttta tggtgcagcc
     1561 accgatgcaa acggcaatta ttcggtcacc ctgcctctga aatcgtataa agacggcttc
     1621 caaatcaaag aagtgtccat taagggtatt ggacaggcag cgtatactca ctattacgaa
     1681 cccaacaaaa caaaaagttt gtccggtgcc tatgctacca ataaacttgc gggtttccct
     1741 atcaacttta ccaatatcac taaggatata ccccattcgt taggtcagca gtttttaaaa
     1801 tttacacccg gtcataacga tggattgccc caggctgatt ttgcacccga atcgtggact
     1861 gacgacctgg caggctggga aaaatatccc ggttttaatg aaacgcttac cgtcacgggt
     1921 acttgctata tggcggctga aacaggcttt gccgtaggct cctacaaaac aggcgttcag
     1981 accgtaaagt taaatgtaaa ctatggtggt gttttgggag ctaaaaccct gtttgctgca
     2041 actaatcccg atggatcgtt cagtatagat ttaccggccg aaaaatcgac agacgtatat
     2101 gcggttgcgc tgcttgctcc ggaagaaaag gaattcttac actataagtc tgctgccgaa
     2161 tctatcaaat tgagcggcgc atacaacctg tacgctaata tcaaaaaaga ggcacccaaa
     2221 tggtatgaat tgggagatca ttattataaa tttacacccg atgctcctca gcccgcaaca
     2281 tggcacggtg acctggccgg atgggtaaag aaaaaagacc ataatctgaa agctaccgtt
     2341 tcgggcaaag tttatttagc taaggaaagt gcttttgcta ccggaaggta tgtgcctgca
     2401 gccaataagg ttattcagat tgatgtcggg acagattctt atcaggggat ggtaagcagt
     2461 gacggcacct tgagtatcga tgtgctgata gaaaacagca gcgacgagtt ggctgttgcg
     2521 cttggtacag gcacaatcaa cgatgttaaa gatttcgaac actatacaaa aggtggaaca
     2581 accaatatca ggaccctgaa aggagactac acggccacac aactgaaatc tgcttcggca
     2641 aaatggaacg aattgggaga tatttattat acatttacac ccgatgctcc cccggcaaca
     2701 tggcaacaag atttagccgg atggcaggta cgcttagatc ataagttttc tgccaacata
     2761 accggcactt tgagacttcc agtagaagaa tcattccgtg tcggagctta ccggggcgca
     2821 gcttatcaga ttgttaaact cgtaagtaac gggtttactc tggttggcgc caccaatgcg
     2881 gaggggaatt tttcaatacc tgttcctctg cagtatgccg atgatgagcc cggagccgct
     2941 tggcacgggg atatggccac attgaaaacc acctcgttct cacacttccg aaaacccgca
     3001 agtggcagta ctgaaatttt agagggtaat tacactttca aacttacgaa tcttcctgcc
     3061 ggctcgcctt ggaatgtact tggtacacgc tattatgatt ttgctcccga cggagcaccc
     3121 accaattggt cgaacaatct gcccggctgg gttgtagtcc ccaattattc tgccgtcata
     3181 atggtaaaag gcaaggttaa aaaagccgtt cagaaaagag aaggcgtttt atggatagca
     3241 ggttgggaag ttgatagaaa ccgcatggta accgttactg ttgcaggaac cccatttgat
     3301 gtggttacca gttctaacgg agatattgta ttcgtatatc ctgctaataa cgctccggat
     3361 aacctgacta tcaccattac tcccgataac gacgacaccg atgttccttt tgtacatcat
     3421 ccggacgtta caaagactaa ttatgtctca atttatggaa agtttgtaag tgcaaacaac
     3481 atagctgcca aaagtatagc cagagatcca agttccagct tatacaatgt gacagagtct
     3541 gcgaaaatgc tatactctcc tgtgggatca ccggatggat ggggatctta tgactgggca
     3601 tccattctcg accaagaata aaagtaaatt gtaatcgtat gaaaaatata aaaattaaaa
     3661 caacacttgt actcgcgatc tgttgtttag cgtggatgtc gcaagcacaa aatgtggagc
     3721 aaaagcaaaa acctatccta ccgaaggcag gagacatcgg gttaggtatc gacttggtcc
     3781 ccgttttgcg ttatgcaggt aatatcttta atggtactgc aaataatgcc ttgaatatct
     3841 tcgggggtga acccgcactt cctattggtg taccggctgc gccgaacgta tccatcagcg
     3901 gaaggtatat gcttaccgac gagttttctg tacgtgccaa tgtgggattg atcgggcgta
     3961 atttatcgca acaactgtat gtcagagatg atgtcgcttt tttcaataat cctttgtcgg
     4021 aagctaaaat cacagacacc tataaacaaa gccgttcagg aatgatcttg tctttcggcc
     4081 ctgaatatag aagaggctat catcgaattc aaggctatgc aggtttgaat ttgctttacg
     4141 gctttgaagc acaaagggat gtatatctgt atggtaatgc cgttacggaa atcaaccaaa
     4201 caccttccag aaacaatgct gcttttgccg tacctccggc tcttactcct gcagttcctg
     4261 cctactggac acgaacttac gtaacaggta agtatcagga tgggaacacg cattatgtag
     4321 gattggaggc ggttgtaggt gtagagtatt atattgcatc tcatttgtcg ctgggaggcg
     4381 aagtctcctt gtatgcattg caagcgttta tgagtgattc gtaccaggaa caggaaggat
     4441 ttaatttctt gaccaacaaa gtagagcaaa gaacagaatt gctgtctcca agctccagag
     4501 aatttaatta tggaacagac aacttaggag ctaaactcta tatgatgttc tatttctgat
     4561 taaggaatag gctgatttaa gaaaaggggc tgtctttagt tttgagacgg cccctttctt
     4621 gtttaatcgg ttgacaagtg attttattta tttaccacca atttcaacgc tgcgttatta
     4681 tcgattttca caatataaac acctgtgctt aatctgctga tgttaagact gttgtttgga
     4741 tataccgtct gtcgaaacac cagtgagccg atactgttga agatttgcac gttt
//
LOCUS       DAOSGL010000167         4791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15862_length_4791_cov_4.289907, whole genome shotgun sequence.
ACCESSION   DAOSGL010000167 DAOSGL010000000
VERSION     DAOSGL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15862_length_4791_cov_4.289907"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PKY68_06010"
     CDS             <1..565
                     /locus_tag="PKY68_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOH24624.1"
                     /translation="VTVPRLFLAIIPLAFSVYMIPGLWGAPLKAISAFAPPMFTQDFN
                     LYDQEVHPDFMHFESGMQFAQQQNKPVLLDFSGYGCVNCRKMENAVWQDIRVKNIIEK
                     EYVLITLMVDDKTALPEMIEIEEYGKVRKLRTIGDKWSYLQRYKFGANAQPFYVLLSP
                     EGKPMAPHYAFDDNVDNFLRFLRSGLE"
     gene            715..2082
                     /locus_tag="PKY68_06015"
     CDS             715..2082
                     /locus_tag="PKY68_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOH24625.1"
                     /translation="MSKTKRFILQEEQLPRQWYNIVADMKVKPIPMLHPGTKQPIKEE
                     DLHPIFSKEASRLELNQTDRFIDIPEEVREKYKYYRSTPLVRAYGLEKALDTPAHIYF
                     KNESVSPVGSHKLNTAIPQAYYCKKEGVTNVTTETGAGQWGTALSYACSLYGLELAVY
                     MVKISYEQKPYRRSIMQSYGATVIASPSMSTRAGRDIITRNPNYQGSLGTAISEAIEL
                     ATQTTNCKYSLGSVLNHVFLHQTVIGLEAEKQMEMAGEYPDMVIACFGGGSNFAGIAF
                     PFMKNSLRDDSLKTRFIAAEPASCPKMSKGIFQYDFGDETGYTPLIPMYTLGHTFAPA
                     NIHAGGLRYHGAGAIVSELLRQGRMEAADIKQLEAFDAALLFAKVEGIIPAPESSHAI
                     AATVREALKCKEEGVKKTILFNLSGHGLIDMSSYDRYLAGDLENYSLSEEELQKNFDK
                     LEKII"
     gene            2089..3369
                     /locus_tag="PKY68_06020"
     CDS             2089..3369
                     /locus_tag="PKY68_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOH24626.1"
                     /translation="MDKKQRPETLCVQAGWQPAGGEPRVLPIVQSTTFKYASSEQMGR
                     LFDLEESGYFYTRLQNPTNDAVAAKINALEGGVAAILTSSGQAANYYALFNICQAGDH
                     LVSATSIYGGTYNLFSVTMKKQGIEVSFVDQDASEAEIARAFKPNTKALFGETISNTG
                     LKVLDIEKFARIAHKQGVPLIVDNTFATPINCRPFEWGADIVTHSTSKYMDGHATSVG
                     GAIVDSGRFDWEAQANKFPGLTTPDASYHGIVYTATFGKEAYITKATAQLMRDLGSVP
                     SPHNSFLLNLGLETLHLRMPRHCANALEMARYLQKHPKVAWVNYPGLPGDRYHDLAAK
                     YLPEGSCGVLTFGLKGGRAEAVAFMDRLRLVAIVTHVADVRSSVLHPASHTHRQLTEA
                     QLKEAGIPHDLIRFSVGSEHIDDLISDIEQALTD"
     gene            3381..3800
                     /locus_tag="PKY68_06025"
     CDS             3381..3800
                     /locus_tag="PKY68_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24627.1"
                     /translation="MKKLLLPAIISLFFYSCNTTVFERDTTQWTFVELKAKPSDWQEY
                     VDNDGKNRYYSCSYNVPEIDHNVYNYGAVLCYVDLDGAQQILPYVRHYEDLEGAQWTR
                     TVDFEYSQGKVNLFVTNSDFVVDRPTETMYFRLVLIY"
     gene            complement(3862..4671)
                     /gene="nagB"
                     /locus_tag="PKY68_06030"
     CDS             complement(3862..4671)
                     /gene="nagB"
                     /locus_tag="PKY68_06030"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOH24628.1"
                     /translation="MRVIIQPDYAKMSEWTAYYIASRINRFKPSADKPFVLGLPTGSS
                     PLGTYKKLIELHQQGRLSFDHVITFNMDEYIGIPKSHPESYYSFMWNNFFNHIDIPKK
                     NIHMLDGNAPDLEAECALYEQAIQDFGGIELFLGGIGPDGHLAFNEPFSSLRSKTRVK
                     TLATDTIIANSRFFGNDLNLVPKTTLTVGIQTIMSAREVVVVVSGHSKARAVRHAIEE
                     PINHVWTISILQTHPKGMIVCDEDATDELKHGTYKYFKYIEKDNLDPETLI"
BASE COUNT         1245 a         1133 c         1148 g         1265 t
ORIGIN      
        1 tgttaccgta ccaaggcttt tcctggccat tattcccctg gctttcagcg tttatatgat
       61 acccggcctc tggggagctc cgctcaaagc catcagcgct tttgccccgc ccatgtttac
      121 tcaggatttc aatctttatg atcaggaagt gcatcccgat tttatgcatt tcgaaagcgg
      181 tatgcagttt gctcaacaac aaaacaaacc ggtattgctc gatttttcgg gctatggttg
      241 tgtaaattgc cgtaaaatgg aaaacgccgt atggcaggat atccgggtga aaaacatcat
      301 cgagaaggaa tacgtactta tcaccctgat ggtggacgac aaaaccgcct tgcccgaaat
      361 gatcgagatc gaagaatacg ggaaggtccg gaaactcaga actattggcg ataaatggag
      421 ttatttgcaa cgttataaat tcggagctaa cgcccagcct ttttacgttc tgctgagtcc
      481 cgaaggcaaa ccgatggcgc cccattatgc ttttgacgat aatgtggata attttctgcg
      541 ctttctgcgc agcggattgg aataagccgg aaaaaaacac ttaatgcaaa ttatttttct
      601 ttactttgtc ccgatttttt gaaaattttt aaagccgcga tagcaatgaa tggttattgt
      661 ccggactgag aatcggcggc ttatatttac caatactatt ttattctgaa ttttatgagc
      721 aagaccaagc gttttattct gcaagaggag cagttgcccc ggcagtggta caatattgta
      781 gcggatatga aagttaaacc catacctatg ttgcaccccg ggaccaagca acctattaag
      841 gaagaagatt tgcatcccat tttttcgaaa gaagcttccc gtctggaact taatcaaaca
      901 gacagattta tcgatatacc ggaagaagtg cgtgaaaaat acaaatacta tcgttccacg
      961 cccttagtca gggcctatgg tttggaaaaa gctttagata cgcctgctca catctatttc
     1021 aaaaacgaaa gcgtcagccc cgtgggttcg cacaaactca atacagccat tcctcaggcc
     1081 tattattgca aaaaagaagg agtgaccaat gtgaccaccg aaacgggagc cggtcagtgg
     1141 ggaactgctc tttcgtacgc ttgcagcctc tacgggctgg aactggcagt gtatatggtt
     1201 aagatcagtt acgaacaaaa accttatcgc cgctccatta tgcaaagtta cggggcaacc
     1261 gtcattgctt cgccctccat gtctaccaga gccggtcgcg atatcatcac ccgcaatccc
     1321 aattatcagg gcagcctggg taccgccatc tccgaagcta tagaactggc cacccagact
     1381 actaattgca agtattcgct gggtagtgtg ctcaatcatg ttttcctgca tcagaccgtt
     1441 attggcctcg aggccgaaaa gcaaatggaa atggccggcg aataccccga tatggtgatt
     1501 gcctgtttcg ggggtggctc caattttgcg ggtattgctt ttccttttat gaaaaacagc
     1561 ctcagggacg attcgctcaa gacacgtttt attgctgccg aaccggcttc ctgtcctaaa
     1621 atgagcaagg gtattttcca atacgatttt ggcgacgaaa ccggctatac acctttgatt
     1681 cctatgtaca ctctgggcca tacgtttgcg ccggccaaca ttcacgccgg cggtctgcgt
     1741 taccacggag ccggggccat tgtcagcgaa ctgttgcgcc agggacgtat ggaagctgcc
     1801 gatattaaac aactggaagc ttttgatgct gctttgttgt ttgccaaagt cgaaggcatc
     1861 atcccggcgc ctgaatcttc ccacgccatt gcggctactg tacgcgaagc cctcaagtgt
     1921 aaggaagaag gcgttaaaaa aactattttg ttcaacttga gcggtcacgg cctgatagac
     1981 atgtcttcgt acgaccgcta cctggccggt gacctggaga attacagttt gagcgaggaa
     2041 gagttacaaa agaatttcga caagcttgag aaaataattt gagcggctat ggataaaaag
     2101 caaaggccag aaacactttg tgtgcaagcc ggttggcaac ccgccggcgg agagccccgt
     2161 gtgttaccca tcgtacagag cactaccttt aaatacgcca gtagtgagca gatggggcgt
     2221 ttgttcgatt tggaagaaag cggttatttc tacacccgtc tgcaaaatcc taccaacgac
     2281 gccgttgctg ctaaaataaa tgccctcgaa ggtggagtag cggccatact gacctcttcg
     2341 ggtcaggcgg ccaattatta cgcccttttc aatatttgtc aggccggcga tcacttggtc
     2401 agcgccactt cgatttatgg aggcacctac aatctctttt cggtgactat gaaaaagcag
     2461 gggattgagg ttagctttgt cgatcaggac gcctccgaag ccgaaattgc ccgggccttt
     2521 aaacccaata ccaaagctct atttggtgaa accatttcca atacgggact taaagtgctt
     2581 gacatagaaa aatttgcgcg gattgcccac aagcaggggg tccctctcat tgtcgacaac
     2641 acctttgcca cccccatcaa ttgcaggcct tttgagtggg gggccgatat cgtgactcat
     2701 tccaccagca aatatatgga cgggcatgcc accagcgtgg gcggagccat tgtagacagc
     2761 gggcgttttg attgggaagc tcaagccaat aaatttccgg gtctgacaac tcccgatgct
     2821 tcctatcacg gcatcgttta tactgccact tttggcaaag aagcttacat caccaaagca
     2881 accgctcaat tgatgcgcga tttgggctct gtgccttctc cccacaattc gtttttgctg
     2941 aatctggggc tggaaacgct gcatctgcgc atgcccaggc attgtgccaa cgccctggaa
     3001 atggctcgtt atctgcaaaa acaccctaaa gtggcctggg taaattatcc cggtctgccg
     3061 ggtgaccgct accacgatct ggctgccaaa tacctgcccg agggcagttg cggcgtactt
     3121 acttttggtc tcaaaggcgg cagggcagag gccgttgctt ttatggacag actccgcctg
     3181 gtagctatag ttacccatgt ggcggatgtt cgcagcagtg tgctgcatcc ggccagtcat
     3241 acgcaccggc aattgacgga agctcagctt aaagaagcag gcattccgca cgatctaatt
     3301 cgtttctcag ttggaagtga acatattgac gatctcattt cggacataga acaagcctta
     3361 accgattaat tgtgaacgct atgaaaaaac ttttattacc ggcaataatc agtctcttct
     3421 tttattcttg caatactact gtatttgagc gtgataccac ccaatggacc tttgtcgaac
     3481 tgaaagccaa accttccgat tggcaggaat acgtagacaa cgacggcaaa aaccgttatt
     3541 attcctgttc gtacaacgtg cccgaaatcg atcacaatgt gtacaactac ggtgctgtat
     3601 tgtgttacgt cgatttagac ggagctcagc aaatcttgcc ttatgtaagg cattacgaag
     3661 acctggaagg agcacagtgg accagaacgg tcgattttga atattcccag ggcaaagtga
     3721 acctctttgt gaccaattcc gattttgtgg ttgatcggcc caccgaaacc atgtatttcc
     3781 gtttggtttt gatttattaa ggccacaact gcaaccgtaa cgctaatcac agcagcccca
     3841 taagctctgc tgacctgaga cttagatcaa agtctcgggg tcgagattgt ctttttcgat
     3901 gtatttgaag tatttgtagg taccgtgctt gagttcgtcg gtggcatctt catcgcagac
     3961 aatcatgcct ttgggatgcg tttgcagaat actgattgtc cagacgtggt taatgggttc
     4021 ctcgatggca tggcgcacgg ctctggcttt gctgtgtccg ctcaccacta ccaccacttc
     4081 acgggcagac ataatggttt gtattcctac ggtcagggtt gttttgggaa ccaaattaag
     4141 gtcgttcccg aaaaagcgcg aattggcgat gatggtatca gtggccaggg ttttgactct
     4201 ggttttggat ctcagcgacg aaaagggttc gttaaaggct aaatggccgt cggggccaat
     4261 gccgcccaaa aacagctcta ttccgccaaa atcctgtatg gcttgttcgt acagggcaca
     4321 ttccgcctcc aggtcggggg cattcccgtc gagcatatgt atattctttt tgggtatgtc
     4381 gatgtgattg aaaaagttgt tccacataaa cgaatagtaa ctctcggggt gtgatttggg
     4441 tatgccgatg tattcgtcca tattgaaagt gataacatga tcaaacgata gtcggccttg
     4501 ttgatgcaat tctatcagtt ttttataagt gccgagcggc gaggagccgg taggcagccc
     4561 caacacaaaa ggcttgtcgg cactcggctt aaaccgattg atgcgtgaag cgatataata
     4621 agcagtccat tcggacattt tggcgtagtc gggttgaata ataactctca tgactcgtta
     4681 gttttaaaat ttacattggc ctcggaatat cacaaggatt gtaaggtctg tttgaaaaac
     4741 acccataaag aggtgcaaag aaaagaaaag tttttgacaa tcgaattttc t
//
LOCUS       DAOSGL010000168         4785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_15892_length_4785_cov_3.774841, whole genome shotgun sequence.
ACCESSION   DAOSGL010000168 DAOSGL010000000
VERSION     DAOSGL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15892_length_4785_cov_3.774841"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PKY68_06035"
     CDS             complement(<1..1119)
                     /locus_tag="PKY68_06035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOH24629.1"
                     /translation="MDIQSILTPVWDYLKNILAYNEDTPLLFTQFYFWAFFALVFALF
                     SLFYKRKQLRTVFLLFASLFFYYKTSGLCLLILLFCITSDYFLAQGIYRSRKQSGKTF
                     LLVVSVSVNMALMAYFKYAYFFTYLVNSLLGTDFVVQDVFAIVGNKLAGNPVFNVDKI
                     VLPVGISFYIFQTLSYTIDVFRGKLKPLNNILDYAFYVSFFPQLVAGPIVRASHFIPQ
                     IYRDFRLSRMQFGMAVFWILNGLVKKIVLGDYIAINFIDRVFANPLMFSGFENLSALF
                     GYSLQVYADFSGYTDIAIGVALLMGFYLPKNFNSPYKATNASNFWKRWHISLSSWLRD
                     YLYFPLGGNRRATFGTYFWILLIALLVVLMSGDIWVFVL"
     gene            complement(1155..2714)
                     /locus_tag="PKY68_06040"
     CDS             complement(1155..2714)
                     /locus_tag="PKY68_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24630.1"
                     /translation="MLALIGLFFPKEGIQLGTVGLKFRSPRDVFVLSGEQVRAPDPEE
                     QLAAMAESFLREKLEALSDSMDYYERFFKESPARIHFPNDDPGMLDLFFETLDSCRIQ
                     KRVVRVLHYGDSQIEQDRLSATLRDGLQKMAGGMGVGMIPAIQTIPSRTLRQEFEGNG
                     KRYVLYGPSSLRAPHKRYGPMAAVCEVEDYAYLSVNALKRQNSDLSNQFTNIRLLLGN
                     NSAGFKATLEIKDKYDTKTVEEAGLDLKVISWELDRPENKFGLFLDGSAEIYALLLDG
                     AYGVAVDNIPMRGSSGNVFTGIDGRLLHAAYRAMDVQLFLLEFGGNRVPSIYSREGIN
                     NYSIAIGRQIRYLKTLVPGAQVIFIGPADMSCLVDGKMQSYPLLEEFIEGLKKAVLEN
                     GAAFWDMFQVMGGQNSMINWVNARPPLAAPDYIHFTKKGADKIAELLSENMALCYSYY
                     QFRKKHNPNDSTRLGLSSLEWVYSDYAEEFEQVIREKLASADSLSEESDNEAILNDFL
                     ELLNKNTNNSE"
     gene            complement(2832..4730)
                     /locus_tag="PKY68_06045"
     CDS             complement(2832..4730)
                     /locus_tag="PKY68_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH24631.1"
                     /translation="MKKKTIVDLFEESCAKYAQNVFLWERDTEFRPTTFAETQKEVYR
                     FGAGLLAMGLKKDEKVALLSEGRNKWMIGELGILYTGAVNVPLSIKLQSGSDLVFRIN
                     HSDSRFILVSGTQLPKVREIMDQMPLIEKVIVMDEDITLQDKELYFEDVCKMGDKFNK
                     KHPGKLLEIAQSVQGDDYANISYTSGTTADPKGILLTHRNYTANVEQSRSRADIPPHY
                     RMLMILPLDHCFAHVTCFYVFMSCGASIATVPVGKTALESLRNIPLSIQEVKPDLMMS
                     VPALAKSFKASVEKGVAAKGPAVQKLFDFAMKVAVAYNKEGYNKGTGGTFVLKPLVKL
                     FDKILFSKLRDALGGNMKFFIGGGALLDIDMQKFWYAIGIPMFQGYGLSEATPVISTN
                     FFTKHKLGSSGVPVIPLELTIRDEKGNILPTGQKGEIVIKGENVMAGYWKNPTATAET
                     IKDGWLYTGDMGAMDEDGFLYVYGRFKSLLIASDGEKYSPEGIEESLVTLSPYIDQVV
                     LYNNQSPYTVALIVVNKELLKKDVPSYDSLEGKKQALKLIQQEINAFKQGGVHEGMFP
                     ERWLPATFAVLPEAFTEQNKLLNSTMKIVRGKVEERYSDRIEHMMTAAGKDIFNEKNI
                     EALRQQLM"
BASE COUNT         1382 a         1158 c         1012 g         1233 t
ORIGIN      
        1 caacacaaac acccagatat caccgctcat caacacaacc agcaaagcaa tcaacaatat
       61 ccaaaaataa gtcccaaaag tagcccgtcg attacctccc aatgggaaat aaagataatc
      121 cctgagccag gaagaaagtg aaatatgcca acgcttccaa aaattggagg cattggttgc
      181 cttgtaagga gaattgaagt tcttaggcag atagaaaccc ataagcaagg ccacccctat
      241 ggcaatatcg gtgtaaccgg aaaaatcggc atagacctga agtgaataac caaaaagcgc
      301 cgacaaattt tcgaatcccg aaaacatcaa aggattggca aaaactctgt cgataaaatt
      361 gatcgctatg taatcgccca atacaatctt cttgaccaaa ccgttgagta tccaaaagac
      421 cgccattcca aattgcatac gggacagtct gaaatccctg taaatttgag gaataaaatg
      481 agaagccctg acgataggcc cggctacgag ctgggggaaa aaactgacgt aaaaggcata
      541 atccaagatg ttgttgagcg gttttaattt ccccctgaat acatcaatgg tataactcag
      601 ggtctgaaaa atatagaaac tgatcccaac cggcaacact atcttatcaa cgttaaacac
      661 aggatttccg gccaatttgt tcccaacaat ggcaaaaaca tcctgcacaa caaagtcggt
      721 tcccaacaag gaattcacca gataggtaaa aaaataggcg tatttaaaat aggccatcaa
      781 agccatattt accgatacac taactaccag caaaaaggtt tttcccgatt gtttgcgaga
      841 acgataaatg ccttgagcca aaaaatagtc ggaagtaata caaaacagca ggatcaacag
      901 acatagaccg ctggttttgt aataaaaaaa caagctggca aaaagcaaaa aaacagtacg
      961 cagttgcttg cgtttataga acaaactgaa taaagcaaag accaaagcga agaaagccca
     1021 aaaataaaat tgcgtaaaca acaagggagt atcctcgttg tatgccaata tattttttaa
     1081 gtagtcccaa acaggggtca aaatgctttg aatgtccatt aatctgctta atatcaagcc
     1141 ttcaggcaat ttatttattc gctattgttg gtattcttgt tcaataattc caaaaaatca
     1201 ttcaaaatcg cctcattatc cgattcctcc gacagactat ctgcagaagc cagcttttcg
     1261 cgaattacct gttcaaattc ttcggcatag tcgctgtaaa cccattccag gctgcttaac
     1321 ccaagacggg tgctgtcgtt gggattatgt tttttgcgaa attgataata tgagtaacaa
     1381 agagccatgt tttcgctgag taactctgcg atcttatcgg ctcctttttt ggtgaaatga
     1441 atgtaatcgg gggcggccaa aggcggccgg gcgttcaccc aattgatcat ggaattttgc
     1501 ccgcccatca cctgaaacat atcccaaaag gcagcaccgt tttccaaaac cgcctttttg
     1561 agaccctcga taaattcttc caatagagga tagctttgca ttttaccgtc caccaggcaa
     1621 gacatatccg ccgggccgat aaaaatcact tgcgcaccgg gtaccagcgt tttgaggtaa
     1681 cggatctgac gtccaatggc aatggaatag ttattgatac cctcccgaga atatatggaa
     1741 ggtacacggt tgccgccaaa ttcgagcaaa aacaactgca catccatagc ccggtaggcg
     1801 gcgtgaagta agcgaccgtc tattccggta aacacattcc ccgaactccc acgcatggga
     1861 atattatcga ccgccacgcc gtaggctcca tccagcaata gggcgtagat ttcggccgaa
     1921 ccgtctaaaa acaagccgaa tttgttttcc ggacgatcga gctcccagct tattactttt
     1981 aaatcgagac cggcttcttc aacggttttg gtgtcgtatt tatcttttat ctcgagagtg
     2041 gccttgaagc cggcagaatt atttcccaac aataaacgta tgttggtaaa ctgattggaa
     2101 agatcggaat tctgacgttt gagcgcattc acactcaaat aagcataatc ctccacttca
     2161 caaaccgcag ccatggggcc gtagcgcttg tgaggagctc tcaggctcga ggggccgtaa
     2221 agaacgtaac gtttgccgtt tccttcaaat tcctgtcgga gagttctgga aggaatggtt
     2281 tgtatcgcgg gaatcatacc cactcccata ccaccggcca ttttttgcag gccgtctctc
     2341 agggtagccg acaaacgatc ctgttctatc tgggagtcgc cgtaatgcaa cacccgtacc
     2401 actctttttt gaatccggca agaatccaag gtttcaaaaa acaagtcgag catgcccgga
     2461 tcgtcgttgg gaaaatgtat gcgtgcgggg ctttctttga aaaaacgctc ataataatcc
     2521 atcgaatcgg acaatgcttc cagtttttct ctcaaaaacg attctgccat ggcggccagt
     2581 tgttcttcgg ggtcgggagc tctaacctgc tccccggaaa gaacaaatac atcccggggc
     2641 gaacgaaact tcaaaccaac agtgcctaat tgaattcctt ctttggggaa gaataaccca
     2701 attaaggcca acatagccat cacaacgagg atgaaacaaa gtattctccg gacagacata
     2761 aggttggatc aagcccttta ttaaggggct tgtgtaaatg aaacaaagta aaataagttt
     2821 ctgctcaaat tctacatgag ctgttgccgc aaagcctcaa tgtttttttc gttgaaaata
     2881 tctttgccgg cagccgtcat catatgttct atgcggtctg agtacctttc ctccacctta
     2941 ccgcgaacaa tcttcatggt agagttcaac aacttattct gctcggtgaa ggcttcgggc
     3001 aaaacggcaa aagtggcagg caaccagcgc tccggaaaca tcccttcgtg tactcctcct
     3061 tgtttaaagg catttatttc ctgctgaatc agttttaagg cttgcttttt gccttcgagg
     3121 ctgtcgtaag agggtacgtc ttttttgagc agttccttgt tgactactat caaggccacc
     3181 gtataggggc tttggttgtt gtacagtacc acctgatcaa tatacggaga caaagtaacc
     3241 agagattctt ctatgccttc ggggctgtac ttttcgccat cgctggcaat cagtaaactt
     3301 ttgaagcgtc cgtacacgta tagaaagcca tcttcatcca tagctcccat atcgccggtg
     3361 tacagccagc cgtctttgat ggtttcggca gttgccgtcg gattcttcca ataaccggcc
     3421 attacatttt cgcctttgat cacaatttcg cccttctgtc cggtaggaag aatgtttcct
     3481 ttttcgtcgc ggatggtcag ctccagagga ataaccggaa ctcccgaaga gcccagtttg
     3541 tgcttagtaa agaaattggt cgaaataacc ggtgtggctt ccgatagacc gtaaccctga
     3601 aacatgggaa taccgatggc ataccagaat ttttgcatat caatgtcgag cagggcgcct
     3661 ccgccaatga aaaacttcat attacctccc agggcatccc gcaacttcga gaacaaaatc
     3721 ttatcgaaga gtttcaccaa aggtttcaga acgaaagttc ctccggtgcc tttgttgtag
     3781 ccctctttat tgtaagctac ggctactttc atggcaaaat cgaataattt ttgtacggcc
     3841 gggcctttgg cagccacccc cttttccacg ctggctttga aactcttggc caaagccggc
     3901 accgacatca tcaaatcggg tttgacttcc tgaatagaca agggaatatt tctcaaagac
     3961 tccagggcgg ttttgcccac cggaacggta gcgatactgg ctccgcaact cataaaaaca
     4021 taaaaacagg tcacgtgagc aaaacagtgg tccagaggca ggatcatcag catacggtaa
     4081 tgcgggggaa tatcggcccg ggagcgcgac tgttccacat tagccgtata attgcgatgc
     4141 gtcagcaaaa tacccttggg atcggccgtt gtccccgatg tataggaaat attggcgtaa
     4201 tcgtcgccct gaacgctttg ggcgatttcg agcaatttgc ccggatgctt cttattgaac
     4261 ttatccccca ttttgcaaac atcctcaaag tagagctcct tgtcttgcag ggttatgtcc
     4321 tcgtccatga cgatcacctt ttcgatcaaa ggcatttgat ccataatttc gcgaactttg
     4381 ggcagttgag tccccgaaac cagtataaaa cgggaatcgg aatgattgat tctgaatacc
     4441 aggtcgcttc cgctttgcag tttgatggac aagggcacat tgacggctcc ggtgtacaaa
     4501 atacccaact cgcctatcat ccatttgttg cggccttccg aaagcaaagc cactttttca
     4561 tcttttttca ggcccatggc cagtaatccg gctccaaaac gataaacctc tttttgggtc
     4621 tcggcaaaag tggtaggccg gaattccgta tcccgttccc atagaaaaac attctgcgca
     4681 tacttggcgc aactttcttc aaacaagtct acaatggttt tctttttcat gactgttatg
     4741 attttatggt ttaaattctt gttcggccaa ggccttgccc ataga
//
LOCUS       DAOSGL010000169         4759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16024_length_4759_cov_4.556335, whole genome shotgun sequence.
ACCESSION   DAOSGL010000169 DAOSGL010000000
VERSION     DAOSGL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16024_length_4759_cov_4.556335"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKY68_06050"
     CDS             complement(<1..470)
                     /locus_tag="PKY68_06050"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOH24632.1"
                     /translation="MIIIIADDLTGAAETVGICLRHGVGAAMIMEIPGEERLQEYTKE
                     GIAVLVIACDTRSGTLEEACRVNRLLGETLKQAGFTQIFKKIDSVLRGWVVPELNTLA
                     RLTGKSSLLIQPANPGAGRCIRDGKYFINGIELSETAFGLDPEFPASSSDVMEIL"
     gene            complement(467..1513)
                     /gene="pdxA"
                     /locus_tag="PKY68_06055"
     CDS             complement(467..1513)
                     /gene="pdxA"
                     /locus_tag="PKY68_06055"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOH24633.1"
                     /translation="MNKTNNTKPVIGISMGDPAGIGPEIIVKALSSAEVYAACRPVVV
                     GDAGVMRQAVQLLGKDLKIHPVQSIPEARFEYGTIDVYDLHNVDLSKLQHGVVSAMAG
                     GAAFEAVRCLIDLAMTSQVDATVTAPINKESIHKAGHAFSGHTEIYAHYTNTKNYAML
                     LADDALRVIHVSTHVSLRQACDRVTKSRVLDVIELLNKACVQFGIARPRLAVAGLNPH
                     AGENALFGDEEVKEIVPAVEEAQRRGYLVEGPLPPDTVFTKAVQGRYDGCVAMYHDQG
                     HIPFKLLGFKWNNETQKMDSVKGVNITLGLPIIRTSVDHGTAFEIAGKGIACEDAMLI
                     SIDYALKMAKNRIQ"
     gene            complement(1519..2667)
                     /locus_tag="PKY68_06060"
     CDS             complement(1519..2667)
                     /locus_tag="PKY68_06060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOH24634.1"
                     /translation="MRSIELIQPKHIIFGAGCAQDWVDIVLAGKKQKVLLLSTHPLWA
                     VLEKSIGNAREKGLELLCLDYSWQAEPDTEIFENLHPQSKDFGPQAIVAIGGGSVLDV
                     GKLLAALSSGTQKPSDVFGKNLLDSRDNLLICVPTTAGTGSEVSPNAILFDLRDGEKK
                     GIISPWLIPDTALIDPEMTFSLPPKLTAETGMDALCHCIEAYTNKYAHPAVDLYALKG
                     IEYVYKNLKTAVKEGNNIEARSAMALASMYGGLCLGPVNTSAVHALSYGLAGKYHFSH
                     GLANAVLLPAVMLFNMEADLEKNARIALAMGLPDKGDLKATARSGIKAIQELSRDCGI
                     PQSLSSLGVKREDLPQLADMAMNVKRLLNNNPRELEWKDALSIYQSIV"
     gene            complement(2674..4224)
                     /locus_tag="PKY68_06065"
     CDS             complement(2674..4224)
                     /locus_tag="PKY68_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOH24635.1"
                     /translation="MNSIHWIDLGIVLISVLLTVFVGIYFAKHQKSSDNYFAGSKRIP
                     SWAIGLSIFATLISSVTFLAYPAAAYKGNWILLVQGLMVPVVLVFLIWLIVPLFRKMI
                     RLSTYEYFERRFGFLARIYSSLAFILTHFSKMGTVLYLVSLALHSMIGVNVIWVIWFL
                     GIAIVLLTLLGGMEGVIWMDVIQGILLICGGLLCAGILLFRPEGGPVSVLTEAITTKK
                     IGFGPYDFNFAELTFWVMAINGIFYALQKYGTDQTIVQRYLTAKTDRDAKKAAYIGVG
                     LSVPIWTLFMLIGTALYFFYHSGTAVLPEGLKADEVFPYFIINELPVGIVGLVIAALV
                     AAAISSLDSDMNCLAAIGVEDYYQRLKPNCTDKQRLNMGRILVAVSGLASIGVASLYA
                     VWEGEGVLGVIFELYAIFSAGIVGIFLLGLFSKRANKQGLYIGMLVCVLFTAYAVLTS
                     TSVEVGGSKRLILDLGSMNFPHHKYMLGVYSHLIVLVVGYVASLFFPKPQLEKGLTIF
                     DYKKPGKN"
     gene            complement(4217..>4759)
                     /locus_tag="PKY68_06070"
     CDS             complement(4217..>4759)
                     /locus_tag="PKY68_06070"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOH24636.1"
                     /translation="YLTLADSLSVPVLMYNIKATTQMSLPLDLVAELSQHPNIAGLKD
                     SERDPARLNECIQKYKNRSDFSFFCGWGAQGANSLILGADGIVPSTGNVVPEMYYKLY
                     KAVLAGDTAAALHYQQQTDRVAELYQKGRNLGSSLAALKVLMKARGLCGTTMMPPLTA
                     LSATEEKSITEQFNKLERNE"
BASE COUNT         1263 a         1222 c         1078 g         1196 t
ORIGIN      
        1 agtatctcca ttacatccga cgaagaggca gggaactcag gatccaggcc aaaagccgtt
       61 tcgcttaatt caatcccgtt aataaaatac tttccgtcgc gaatgcaacg tccggctccg
      121 ggattggccg gctggatcag caaactgctt ttgcccgtca atcgggctag tgtattcagt
      181 tcgggtacta cccagcctcg caatacggag tcgatttttt tgaagatttg cgtgaatccg
      241 gcctgtttga gcgtttcacc cagtaaacgg tttacccggc aggcctcttc taaggtgccg
      301 gatcttgtat cacaggcaat taccaataca gctattccct cttttgtata ttcctgcaaa
      361 cgctcttcgc ccgggatttc cataatcatg gccgccccga ctccgtgacg caaacagata
      421 ccgacggttt cggctgctcc ggttaaatcg tctgcaataa tgataatcat tggattctat
      481 ttttggccat tttcaaggcg taatcgatcg aaatgagcat agcgtcttcg caggctatcc
      541 ccttgcccgc aatttcaaag gcagtgccgt gatccacaga ggttctgatg atgggtaagc
      601 ccaaggttat attgacgcct ttaacactat ccattttctg ggtttcatta ttccacttaa
      661 atcccagcaa cttaaagggg atatgtccct ggtcgtggta catggctaca caaccgtcgt
      721 aacggccctg caccgctttg gtaaacacag tatccggagg caggggccct tccaccagat
      781 aacctcgccg ttgagcttcc tctaccgccg ggacgatttc tttgacctct tcatcgccaa
      841 acaaggcgtt ttcgccggcg tggggattca ggccggctac cgccagacga ggcctggcaa
      901 taccaaattg cacgcaggcc ttgttcaaaa gctctattac atcgagcacc ctcgatttgg
      961 ttacgcggtc gcaagcctga cgcaaagaca cgtgagtact gacgtgtatc actctgaggg
     1021 cgtcatcggc cagcagcatg gcgtaatttt tggtgttggt gtaatgagca taaatttcgg
     1081 tatgtcccga aaaggcgtgt ccggccttgt ggatagactc cttgttgatg ggcgccgtaa
     1141 cagtggcatc cacctgagaa gtcatggcca gatcgatcag gcaacgcacc gcctcgaaag
     1201 ccgccccgcc cgccatagca gataccacac cgtgttgtaa cttgcttaaa tcaacgttat
     1261 gcaaatcata cacatctatg gtgccgtact caaagcgagc ctccggaatg ctttgaaccg
     1321 gatgaatttt caggtccttg cccagtaatt gtacggcttg tcgcataact ccggcatctc
     1381 ctaccaccac gggccggcag gcggcataaa cctccgctga agataaagct ttcacaatga
     1441 tctcggggcc gataccggcg ggatcgccca tgctgattcc tataacgggt ttggtattat
     1501 ttgttttgtt cattgcgatc atactataga ttgataaata ctcagggcat ctttccactc
     1561 aagttctctg ggattattat tcaacaagcg cttgacgttc atggccatgt ctgccaactg
     1621 cggtaaatct tcccgtttaa ctcccagcga agacaagctt tgaggaatac cgcaatcgcg
     1681 ggaaagttct tgtatggctt ttattcccga ccgagccgta gccttcaggt ctcctttatc
     1741 cgggagaccc atagccaaag ctattcgagc atttttttcc aaatcggctt ccatgttgaa
     1801 caacattacg gcaggcaaca aaacggcgtt ggctaaaccg tgggaaaaat gatatttccc
     1861 ggccaggccg taggataagg catgcactgc cgaagtattt accgggccca gacaaagtcc
     1921 tccatacata gaagccaatg ccatggcaga gcgggcttca atattgttgc cctcctttac
     1981 cgctgttttc agatttttgt aaacgtactc aattcctttg agggcgtaca gatctactgc
     2041 cgggtgggcg tatttgttgg tataagcctc aatacagtgg cataaagcat ccatgcccgt
     2101 ttcggcagtg agtttcgggg gcaggctaaa agtcatctcc ggatcgatca atgccgtatc
     2161 gggtatcagc catgggctga tgatgccttt tttctcgccg tcgcgcaaat cgaacaaaat
     2221 ggcgttgggc gatacttcgc tgccggttcc cgccgtggtg ggaacacaaa tcaacagatt
     2281 gtcccggctg tcgagcagat ttttaccaaa aacatcggag ggcttttggg tacccgaaga
     2341 aagggcggcc aacagcttgc ccacgtcgag cacgctgccc ccgccgatgg ccacgattgc
     2401 ctgggggccg aagtctttgc tttgcgggtg caggttttca aagatttccg tgtcgggctc
     2461 ggcttgccag gaataatcca gacacaacaa ctccaaccct ttctcgcgcg cgttcccgat
     2521 gcttttttct aaaacagccc aaaggggatg agtagataaa agcaacacct tttgtttttt
     2581 cccggccaat acaatatcca cccagtcttg tgcacagccg gcgccaaaga ttatatgttt
     2641 gggttgtatc agttcaatgc ttctcataat cagttaattt ttgcccggct ttttgtaatc
     2701 gaaaatggtc aggccctttt ccaattgggg tttcgggaaa aacaaactgg ccacataacc
     2761 cactaccaat acaatcagat gactgtaaac gcccaacatg tacttatgat ggggaaagtt
     2821 cattgagcct aaatcgagaa tcaggcgttt gctgcctcca acctcgacgg atgtactggt
     2881 taggaccgca taggccgtaa ataacacaca aaccagcatg ccgatgtaca aaccttgttt
     2941 gttggccctt ttgctgaaaa gccccaatag aaagataccg actatcccgg cagagaaaat
     3001 ggcgtataat tcaaaaatca cacccagcac tccttcaccc tcccaaacgg cgtaaagact
     3061 ggctacacct atggaggcca agcccgaaac ggcaaccaat attctgccca tattcagccg
     3121 ttgtttatcg gtgcagtttg gtttcaaacg ctgataataa tcttccaccc cgatggcagc
     3181 cagacaattc atgtccgaat cgaggctgga aatggcagca gccaccaagg ctgcgataac
     3241 cagaccaaca atacctacag gtagttcgtt tatgataaaa tagggaaaga cttcgtctgc
     3301 tttcaaacct tcgggcagta cggctgttcc ggaatgatag aaaaaataca aggcggtacc
     3361 tatcaacata aacaaggtcc agatgggcac actgaggcct actccgatgt aagccgcttt
     3421 tttggcatct ctgtcggttt tggcagtcag atagcgttgt actatagtct gatcggtgcc
     3481 gtatttttgc aaggcataaa aaatcccgtt gatagccata acccaaaaag tcaattcggc
     3541 aaaattgaag tcatagggac caaagccaat cttcttggta gtgatggctt ccgtaaggac
     3601 cgacacaggt cctccttcgg gcctgaaaag caatatcccg gcacaaagca ggcctccgca
     3661 tatcaataaa attccctgaa taacgtccat ccatatcaca ccctccatac cccctaacag
     3721 agtaagcaac acaatggcta tacccaaaaa ccaaataacc cagattacat tgacacctat
     3781 catgctgtga agtgccagac tgacaagata caacacagtg cccattttcg aaaagtgggt
     3841 aaggatgaag gctaatgaac tgtaaatccg ggccaaaaat ccaaaacgcc gttcaaaata
     3901 ttcgtaggta cttaaacgaa tcattttgcg gaataaagga acaatcagcc atatcagaaa
     3961 taccaacact accggaacca tcaggccttg taccagcaaa atccagttgc ctttgtaggc
     4021 ggcggcagga taagccaaaa aggtaacact gcttatcagc gtagcaaaaa tagataagcc
     4081 tatagcccag gaagggattc ttttgctgcc ggcaaaataa ttgtcggaac tcttttggtg
     4141 tttggcgaaa taaataccaa caaaaacggt tagtagcaca cttatcagga caatgcccag
     4201 atctatccaa tgaatactat tcatttctct ctaatttatt gaattgttct gttattgatt
     4261 tttcttctgt agcagacaaa gcagtcaggg gaggcatcat agtagtgcca cacaagcccc
     4321 tggctttcat cagcactttt aatgccgcca gggaactgcc cagattacgg cctttttgat
     4381 acaattccgc cacacgatcg gtctgttgct gataatgcaa agcggcagcg gtatcgccgg
     4441 caagcactgc tttatataat ttataataca tttccggaac cacattgcct gtgctgggga
     4501 cgataccgtc ggctccgagt atcaaactat tcgctccttg cgcaccccag ccgcagaaaa
     4561 aagaaaaatc ggaacgattt ttatacttct gtatgcattc gttcaaacgt gcgggatccc
     4621 tttcggaatc tttcaatccg gcaatattgg ggtgttggct caattcggct accaagtcca
     4681 gtggaagcga catttgggtt gttgccttga tattgtacat gagaaccggc acggataaag
     4741 aatcggccaa ggtcaggta
//
LOCUS       DAOSGL010000170         4755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16043_length_4755_cov_4.770851, whole genome shotgun sequence.
ACCESSION   DAOSGL010000170 DAOSGL010000000
VERSION     DAOSGL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16043_length_4755_cov_4.770851"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PKY68_06075"
     CDS             <1..727
                     /locus_tag="PKY68_06075"
                     /inference="COORDINATES: protein motif:HMM:NF028443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5108 domain-containing protein"
                     /protein_id="HOH24637.1"
                     /translation="FVRSDIKASNGYIHKIDGILPVWCPEPSVVVWDFCNSTDIVAIA
                     NSYGAANNLGNLYSTPSDAAEYKVDITKSNPYGTIHSFDYKLASTKGTWLRIGYWKCK
                     ANTDATLDYENEFNAHLNNLLILNLGYTGWIEFTTPTIVKGTYRVELYYAGAKALAKK
                     FYGGGSAVKFTLDDFLKQHYLWKGWESSQQAVQGEVLFPNITFETTQSHSFKAVFMDA
                     NASTISTYRQMWDYVKFIPLAEE"
     gene            755..2566
                     /locus_tag="PKY68_06080"
     CDS             755..2566
                     /locus_tag="PKY68_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24638.1"
                     /translation="MKKHQSILILSLLSLTLAFTACGDAWDNHYGKQTPVADGNVEIY
                     PGSLKEFLASAPEFSTQHALFADAGIYDKMLPGQEYTILLYPDNILDTTSYAQDNSYA
                     AYCICDIALSPADMEEGMGISTWYGKNLWISLQGEQIKINDCPVEAITRTNNAFVYRI
                     NEKVIPVRLSVYDVINGLDDARYSKFKSLIRTYERRYFDPAKCTPAGTNEQGNTIYSD
                     SVKGWSTKNILMDRYSKDGQEMWNMRNEYYNSTVFIPDNDIIQAAVDTALARVPLWLG
                     RTATAADRNKFEKWIIRACFVDRLLNAGQVTGSEDIDCVGGYQKDSLEGKTVFEADMA
                     MWRPWVQKVRTGDMISASNGNIFFVDWLKIPNNVVIYRLKSRFYELWNNMNQEQRDKY
                     FRWDHWIDPMVLNDAQSSFTLSETMPTMYYHVLTAIPDKDARRDSLVCSVTYDGVLFN
                     QNNPRGSQIRECHIPAGEYYLRMGFKHSLLYSLSIQFCDTLLVEDMVMYAQGSNFHFD
                     RGSVSVVDNYGESSIGYPEGYNWHDWSSLSEKAQAYDTDGYPVGIVNMKEDGNFTITI
                     SSSDMSYLYTYGADRNTHNISQLMMYHWCLRPTKNNY"
     gene            2655..>4755
                     /locus_tag="PKY68_06085"
     CDS             2655..>4755
                     /locus_tag="PKY68_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOH24639.1"
                     /translation="MEKSYDIDHAIKGKIPGLQVVGKSGMPGEGAYLNSGGIHSLYAA
                     NTPLIVVNGVPYMYDTKLSTAINGYSRSLFAALNINDIRSITLLTGSDAAKYGSLGSN
                     GVLLIETEQATSDNLNTRISLCTQYGLHFKGRTVDVLDAGGYKNYLRSVGLSRYGQMG
                     PLTTDYPFLQTSSDYPSAYIFNNNTDWQSEIYRPTLVTNNVFRVEGGDEIAKYNMSVG
                     YSSDGGVLYGTRTDRYHTLINSDITVSRKIDIFSSVGLSYLRSQLQEQGMNKETNPLL
                     AAWFKMPVLNPYFAGSDGELTGIYAPYNFSNINDYPQYPYENISNPTAIVNTLEAADK
                     VYDVNILLGLNYQLTNKIKLTAQYNRQYRYTQEDLFIPGTNNQAIYPQYYGIGRNTVR
                     KAVAENRNSFYGLNVSYRNTPNRIHQWEIDAGGRIMSTSAEYDMSSGYNTANDFYKTL
                     DMTTDEEYSDGYISQWLWANYYLNTGYTWKEWLAANLNFSVDGSSVSGIDAPRYYVYP
                     AGALQFLAARLSFIPEWIDRLDLSAEYALTGNSRFSSNYAKNYYNSSNFFAMGSIIRG
                     DIPNTLLEPEKHNQFKAGIDMSLLNKRVQLSAAAFNSYAYDLIIPQNISSIYAADNYY
                     ENSAAVSTDGLIVSVRFVPVETNKFSWTLGGNLTTNKSIVKDLGEQTYMDIFYTQFSE
                     GEDAIVRLEVGETPYQFY"
BASE COUNT         1337 a         1231 c         1054 g         1133 t
ORIGIN      
        1 ctttgtccgt tccgacatca aagcgagcaa cggttatatc cacaaaatcg acggcatttt
       61 acctgtttgg tgtccggaac cctctgttgt ggtgtgggat ttttgcaaca gcaccgatat
      121 cgttgccatt gccaacagct acggtgccgc caacaacctg ggcaatcttt acagcacacc
      181 cagcgatgct gctgaatata aggtagatat aactaaaagc aatccctacg gaaccataca
      241 ttctttcgat tataagctag cctccaccaa aggaacctgg ctcagaatag gttattggaa
      301 atgcaaagcc aatacagatg ctacactgga ttacgaaaac gaattcaacg ctcatctgaa
      361 caatttactc atactcaatc tgggctatac cggctggata gaattcacta cccctaccat
      421 tgtaaaaggt acttaccggg tagaattgta ctatgcggga gccaaggcgc tggccaagaa
      481 attctacgga ggaggcagcg ccgtcaaatt cacgttggac gacttcctga aacagcatta
      541 cttgtggaaa ggttgggagt cgagtcaaca agccgttcaa ggggaggttt tatttccaaa
      601 tatcactttt gaaactacgc aaagccacag cttcaaagct gtattcatgg atgccaatgc
      661 ttccactatt tccacttacc gccagatgtg ggattacgta aaattcattc ctttagccga
      721 agagtaagct atgcacgcaa aaagaacgac aattatgaaa aaacatcaat ccatacttat
      781 cctcagtctt ctaagtctaa cgcttgcgtt taccgcctgc ggcgatgcct gggataatca
      841 ttacggcaaa caaacgccgg ttgccgacgg taatgtggaa atttatccgg gcagcctgaa
      901 agaatttctc gcttccgctc ctgaattcag cactcagcat gctctttttg ccgatgcggg
      961 catttacgac aagatgcttc ccggacaaga atataccatt ttgctttatc cggacaacat
     1021 actggacact acctcctatg cccaagacaa cagctacgcc gcctactgta tctgcgacat
     1081 tgctctgtct cctgccgaca tggaagaggg catgggcatc agtacctggt acggcaaaaa
     1141 cctttggatt agcttgcagg gtgaacaaat aaaaatcaac gactgcccgg tcgaagccat
     1201 tacccgcacc aacaacgcct tcgtttaccg gataaacgaa aaggttattc ctgtcagact
     1261 gtcggtctac gacgttatca acggcctgga cgatgccagg tattcaaaat ttaagtcact
     1321 gatacgcacg tatgaacgca ggtattttga tccggccaaa tgtacgccgg caggcaccaa
     1381 cgaacagggt aataccatct actccgactc cgtcaaaggc tggtcgacaa agaacattct
     1441 gatggaccgc tacagtaaag acggccagga aatgtggaac atgcgcaacg agtactacaa
     1501 cagcaccgtt tttatacccg acaacgacat cattcaggca gccgtagata ctgccttggc
     1561 ccgtgtgccg ctttggctgg ggcgtacagc caccgcagcc gaccggaaca aattcgaaaa
     1621 atggataatc agagcctgct ttgtagatcg actgctcaat gccggacaag taaccggttc
     1681 cgaagacatc gattgtgtgg gaggctacca gaaagacagt cttgaaggta aaacagtatt
     1741 tgaggcagat atggccatgt ggcgtccctg ggtacaaaaa gtccggaccg gcgacatgat
     1801 ttcggccagt aatggaaata tcttctttgt cgattggcta aaaatcccta ataacgtggt
     1861 tatttaccgt ctgaaaagcc gtttctacga gctatggaac aatatgaacc aggaacaacg
     1921 cgacaagtat ttccgctggg atcactggat cgacccgatg gttctgaacg atgcccagag
     1981 ctcctttaca ttgagcgaga ccatgcctac catgtactat catgtactga cggctatacc
     2041 cgacaaggat gcccgccgcg attcgttggt ctgcagcgtt acctacgacg gcgttttgtt
     2101 taaccagaac aatccacgcg gcagccagat acgggaatgt catatccctg ccggcgaata
     2161 ctacctgcgt atgggcttta aacacagctt actttattca ctcagtattc aattttgcga
     2221 caccctgttg gtcgaggaca tggttatgta tgcccaggga tcgaatttcc acttcgaccg
     2281 cggatcggtg tcggtagtcg acaattacgg ggaatcgtcc atcggttatc ccgagggcta
     2341 caactggcac gattggtcgt cgctgagcga aaaagctcag gcctacgata ccgacggtta
     2401 tcccgtcggc atagtcaaca tgaaagaaga cggcaatttt acgattacca tctcatcttc
     2461 cgacatgagt tacctctata cttacggagc cgaccggaat acccacaata taagtcagct
     2521 gatgatgtat cactggtgcc tacgtcccac gaaaaacaac tattgatatg aaaagagttc
     2581 tttcaattag cgtcgtacta gtcttgtgcc tttcgaccgt aatgggacag ggcaatgtta
     2641 tatacacaaa ggacatggaa aagtcttacg acattgacca tgccattaag ggaaaaattc
     2701 cgggtctgca agtagtcggc aaaagcggta tgcccggcga aggtgcctat ttaaattcag
     2761 gaggtatcca ttctctgtat gctgccaaca cccccttgat tgtagtaaat ggtgttccat
     2821 acatgtacga caccaaactt tcaaccgcca tcaacggata cagccgtagt ttgtttgcag
     2881 cccttaatat caacgatatc cgcagcatca ctttattgac cggctccgat gcagccaaat
     2941 acggctctct gggttcaaac ggggtgcttc tcatcgaaac ggaacaggcc accagcgata
     3001 acctgaatac gcgtatttcg ctttgtacac agtacggact gcatttcaaa ggccgtaccg
     3061 tcgacgtact cgatgccggc ggatacaaga attatcttcg ttcggtaggt ctgtcccgct
     3121 acgggcaaat ggggcctctc accaccgatt atccttttct gcaaacttcg tccgactacc
     3181 ccagcgctta catcttcaac aacaacaccg actggcagtc tgaaatttac cgtccgactc
     3241 tggtcaccaa caatgttttc cgtgtagaag gcggcgacga aattgccaaa tacaatatga
     3301 gcgtgggcta ctcttcggac ggaggcgtac tgtacggcac ccggaccgat cgttaccata
     3361 cattgatcaa ctcggacata acggtcagcc gcaaaatcga cattttcagt tcggtcggac
     3421 tctcttactt gcgcagccaa ttgcaggaac aaggcatgaa caaagaaacc aatcccctgc
     3481 tggcagcctg gtttaagatg cccgtactca atccgtattt tgcgggcagc gacggagaat
     3541 tgacagggat ttacgctccg tacaattttt cgaacatcaa cgattaccct caatatccct
     3601 acgaaaacat cagcaatcca actgctattg taaatacact ggaagccgcc gacaaagttt
     3661 acgatgtcaa catcctgctc ggactgaatt accaactgac caacaagatt aaactcacgg
     3721 cgcaatacaa ccggcaatac cggtataccc aggaagacct gtttataccc ggcacaaaca
     3781 accaagccat ttatccccaa tactacggaa tcggacgcaa caccgtacgc aaggctgtgg
     3841 ccgaaaaccg gaacagcttc tacggtctga acgtctctta ccgaaatacc ccgaatcgga
     3901 ttcaccaatg ggagatcgac gccggaggcc gcattatgag tacctctgcc gaatacgata
     3961 tgtcttcggg ctacaatacg gccaacgatt tctacaaaac cctcgatatg accacagacg
     4021 aagaatattc cgacggttat atcagtcagt ggctctgggc caactactac ctcaataccg
     4081 gatatacctg gaaagaatgg ctggcggcca atctaaactt ttccgtggac ggatcttcgg
     4141 taagcggtat cgatgctccg cggtattacg tttacccggc aggcgcctta caatttttgg
     4201 cagcccgtct ttcgtttatt cccgaatgga tagaccggct cgatctgagt gccgagtatg
     4261 ccctgacggg aaacagtcgc ttttcgagta actatgccaa aaactactac aacagctcca
     4321 atttcttcgc tatgggatcc attatacgcg gtgatattcc caataccctg ctggaacccg
     4381 agaaacacaa ccaattcaaa gccggaatcg atatgagttt gttgaacaaa cgcgttcaac
     4441 tgagcgcagc cgctttcaat tcctacgctt acgatttgat tattcctcaa aatatttcga
     4501 gcatctatgc tgcagataat tattacgaga actcagcagc cgtttcgacc gacggcctca
     4561 tagtgagtgt tcgcttcgta cctgtcgaaa ccaacaagtt cagctggacc ctgggcggta
     4621 accttacaac gaataaaagc atagtcaaag atttaggaga acagacttat atggatatat
     4681 tctacaccca attctccgaa ggcgaagacg ccatagtaag actggaagta ggtgaaacgc
     4741 cctatcagtt ttacg
//
LOCUS       DAOSGL010000171         4754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16048_length_4754_cov_4.045329, whole genome shotgun sequence.
ACCESSION   DAOSGL010000171 DAOSGL010000000
VERSION     DAOSGL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16048_length_4754_cov_4.045329"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKY68_06090"
     CDS             <1..448
                     /locus_tag="PKY68_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24640.1"
                     /translation="AFFHFRYGNQIVNESRMRIENMYSNDNQSIAVKWRWRKEGDVTN
                     MPRALYNTGYNWLGSDRYVEDGSFLRFKYLTLNYSIAPNLIKRFGLTDLRFYLTLNNL
                     ALWTNYSGVDPEVGYAATASDPFRIGYDTSKTPRSKDGTFGVTIGF"
     gene            460..1995
                     /locus_tag="PKY68_06095"
     CDS             460..1995
                     /locus_tag="PKY68_06095"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24641.1"
                     /translation="MKRKFSHQILLALGLALHTVLSSCSDEWLEVFPKDMIVYEDFWK
                     TKAQLESAVSGCYMACLTESESYSVAGQMLLWGELRGDNITAGPSVSDDESKIMSFNI
                     LPSNAICNWKRFYTVINNCNVVLENARFVMDSDKSLSIRDVEAYQAEALALRSLMYFY
                     LVRTFKEVPLVLEASTNSEQDFYTPKTTEDSVLTQIVGDLEKAYTMAMSTHDRLEYDK
                     GRFTKTSIAALLADVYLWKEDYENCIRCCDIVLSNKELVMTKDRTGTGMIWRQNIFVY
                     GNSNESILEFQYGNSNQGIVNNALFRSFGSSSRNPHFNVPLDITSWDLNSIFGKKDFR
                     AKMSYTKESGASSGKSTKFQESESSGANWIVYRLPEIYLMKAEALVQLDFETNKQQAL
                     QLVNTVYMRAQTTPTIDDSLQMAVYDEKMLMDDLILAERQRELMFEGKRWFDIMRAAR
                     REKTMRVFSKYILPNVEGSYYELASAKLTKEWARYFPVPLSEMESNSLLTQNPFYETL
                     LDK"
     gene            1997..3850
                     /locus_tag="PKY68_06100"
     CDS             1997..3850
                     /locus_tag="PKY68_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24642.1"
                     /translation="MRTKKSSIVILILCFVLSAGLESCFFDPGENIYTKKLETIGEFF
                     ENDTIFEEFAKVLNETKVMGMLKSYGEYTCFAPTNQAVRNFFANSKFGSLENFPIDSL
                     KNIVLYQLIENDAIQTVDFTSSRLRSNNMVNDQLQVRYDSLGDIVINGMARLLMWDME
                     LRNGVVHLTDAFMLPDTATIDQKFEAEGLKERFSIFNQALKETGIARLMHKTEDQTEY
                     SYYSVKTGAKIIIPRYYKFTIFAESDSTYARLAGIHSLDDLKQWCDNKAYGDPSLPID
                     DPQSSLYRYIAYHCLDRVYYKKDFENYARRDGFGDGYEVIETMYPNTLLEYRKAKGRI
                     VFNPETRNDTIIENTGTFLTTESIYQDIKAGNGVIHELDGMMEIPDQASFFHKKMRFN
                     IASFLPELMNYGARGQKVLELPDARLGGPQYFENMIIYGDAGFVLPRYDYTGSWIRHH
                     WDEILIGMGYQAENWAQCNSATEYANTRYDVHLRIPPVPVGTWEIRFGFTTNQYRGMA
                     QLYFDGIPAGVPLSFLPTENYGISGMDEQTARKVLYNNGFMSFPTHITDASGNNTAYT
                     RTDRMRRILGVFSFSKMEPHVIRLKTVEPGQLSLNFVEYIPIEMVAEEGYD"
     gene            3861..>4754
                     /locus_tag="PKY68_06105"
     CDS             3861..>4754
                     /locus_tag="PKY68_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24643.1"
                     /translation="MKKTVKHIARFLPLLLLAAVACQDSWNRHYSYDKTLGGEDLLTT
                     LKAEASYSRFVKLIEIAGLQNKLAGSGSYTVWAPHNDFIPDSVYTSGDTALIRDVVHN
                     HIAYFLHSTAEAYSGSSVMMLNGKYIHFGKGGSEKFAFGGFELDPADRVCSNGVLHGL
                     AGYVPFEYNLWEYLEKTPELSLLLQAYNFYDSTYMDRERSRRIGVDSMGNHVYDTVLI
                     QENTLLRRIGKIYNEDRNYTMIAPTNRAFEQAFDYYKNYYKSNGGPTVTMNKDSIQRT
                     RTHLALIRNLIFEGGFETLTDT"
BASE COUNT         1314 a         1284 c         1089 g         1067 t
ORIGIN      
        1 tgctttcttc cacttccgtt acggcaacca gattgtgaac gaatcgagga tgcggatcga
       61 aaacatgtac tccaacgaca accaaagcat cgccgtaaaa tggcgctggc gcaaagaagg
      121 cgatgtcacc aatatgccca gagccctgta caacaccggc tacaactggt tggggtccga
      181 ccgttacgtg gaagacggtt ctttcctgag gtttaaatac ctgactttaa attattcgat
      241 cgctcccaac ctgataaagc gatttggcct gaccgatctg aggttttact taaccctgaa
      301 caacctggcc ctttggacca actattccgg agtggatccc gaagtgggct atgcagccac
      361 agccagcgat cccttcagaa tcggatacga tacttccaaa actccacgaa gtaaagacgg
      421 cacctttggt gtcactatcg gtttttaaca ttaagcgcga tgaaaagaaa attctcccat
      481 cagatattgt tagccctcgg cctggccttg cacaccgtgc tcagttcgtg cagcgacgaa
      541 tggcttgagg tgtttcccaa agacatgatc gtctatgaag acttctggaa aaccaaagct
      601 caacttgaat cggccgtttc gggttgttat atggcctgcc tgacggaatc cgaaagctat
      661 agcgtagccg gccagatgct gctctggggc gaactcaggg gcgacaacat tacggcaggt
      721 cccagcgtgt cggacgatga atccaagatt atgagcttca acattctgcc ttccaatgcc
      781 atctgcaact ggaaacgctt ctacacggtc atcaacaatt gcaatgtggt actcgaaaat
      841 gcccgttttg tgatggattc cgacaaaagc ctttcgatca gggatgtaga agcataccag
      901 gccgaagcat tggccttgcg ttcgctcatg tatttctacc tggtacgcac tttcaaagaa
      961 gtacctttgg tattggaagc cagcaccaac agcgagcagg acttttacac tcccaaaacc
     1021 accgaggaca gtgtgctgac acaaatcgtc ggagatctcg aaaaagccta caccatggct
     1081 atgagcaccc acgacaggct ggaatacgac aaagggcgat ttaccaaaac ctccatagca
     1141 gctttgctgg ccgacgtcta cctgtggaag gaagattacg aaaattgcat tcgttgttgc
     1201 gatatcgtct tatccaacaa ggagctggtg atgacaaaag accgtaccgg caccggaatg
     1261 atctggagac aaaacatttt tgtctacggc aactccaacg aaagcatcct cgaatttcaa
     1321 tacggcaatt ccaatcaggg tatagtgaac aacgccctgt tccgcagttt cggttcttcg
     1381 tcgcgcaatc ctcattttaa cgtgcctttg gacatcacat cctgggacct gaacagtatt
     1441 ttcgggaaaa aagatttcag agccaagatg tcttacacca aggaatcggg agcatccagc
     1501 ggaaaaagta ccaagtttca ggaatcggaa tcgtccggtg ccaactggat agtctacagg
     1561 ctgcccgaaa tctacctgat gaaagccgaa gccctggtac aactcgattt cgaaaccaac
     1621 aaacaacagg cactccaact ggtcaatacc gtgtacatgc gcgcccagac cacccccacc
     1681 atcgacgatt cgctgcagat ggccgtctac gacgaaaaga tgctgatgga cgatttgatt
     1741 ctggccgaac gccaacggga actgatgttc gaaggcaaac gctggttcga tattatgaga
     1801 gcagcccgcc gcgaaaaaac catgcgtgta ttcagtaaat atatactacc caatgtagag
     1861 ggatcgtatt acgaattggc ctcggccaag ctcaccaaag aatgggctcg ttatttcccc
     1921 gttcccctgt cggaaatgga atccaattct ttactgacac agaatccttt ttacgaaacc
     1981 ttgttagaca aatagtatga gaacgaaaaa atcatccata gttatattga tcctgtgctt
     2041 tgtcctgtcg gccgggttgg aatcctgttt tttcgatccc ggagaaaaca tttacaccaa
     2101 gaagttggaa accataggag agtttttcga aaacgacact attttcgagg agttcgccaa
     2161 agtcctgaac gagacaaaag tgatgggtat gcttaaatcc tatggggaat acacctgttt
     2221 cgcccctacc aaccaggctg tcagaaactt ttttgccaat tccaagtttg gcagcctcga
     2281 aaatttcccc atcgactcgc tcaaaaacat cgtattgtac cagctcatcg aaaacgatgc
     2341 catacaaacg gtcgatttca cctcttcgcg cctgcgttcc aataatatgg tcaacgacca
     2401 attgcaggtg cgctacgatt cgctgggcga tatagtgatc aacggtatgg cccgcctgct
     2461 gatgtgggat atggaattgc gcaacggtgt ggttcatctg accgatgcgt ttatgttgcc
     2521 cgacacagcc acgatagacc aaaaatttga agcagagggt ctcaaagagc gcttcagcat
     2581 cttcaaccag gcgctcaaag aaaccggcat tgcccgcctg atgcacaaaa ccgaagacca
     2641 gaccgaatac tcctattaca gcgtaaaaac cggggctaaa attatcattc cccgctatta
     2701 taagtttacc atttttgccg aaagcgatag cacctacgcc cggctggccg gcattcacag
     2761 cctcgacgac ctgaagcaat ggtgcgacaa caaggcatac ggcgatccat ccctgcccat
     2821 cgacgatccc caaagcagcc tgtaccgcta catagcctac cactgcctcg acagggttta
     2881 ttacaaaaaa gatttcgaaa actacgcccg gcgcgacggc ttcggagacg gttacgaagt
     2941 cattgaaacc atgtatccca acaccctgct cgagtatcgt aaagccaaag gcagaattgt
     3001 tttcaatccc gaaacccgca acgacaccat catcgaaaac accggcactt ttctgacaac
     3061 cgaaagcatc tatcaggaca tcaaagccgg taacggagtg atacacgaac tggacggaat
     3121 gatggaaatt ccggatcagg ccagcttttt ccacaaaaag atgcgtttca acattgccag
     3181 tttcctgccc gaattgatga attacggagc acgggggcaa aaagtcctcg aattgcccga
     3241 tgcccgccta ggaggacccc agtattttga aaacatgatc atctacggcg atgccggctt
     3301 tgtactgccg cgttacgact acaccggttc ctggataagg caccactggg acgaaatttt
     3361 gataggcatg ggttatcagg ccgaaaactg ggcacagtgc aacagcgcca ccgaatacgc
     3421 caatacccgc tacgacgtcc atctgcgtat tccgcccgtg ccggtaggaa cctgggaaat
     3481 ccgtttcggt tttaccacca accaataccg gggcatggcc cagttgtatt tcgacggcat
     3541 accggctggt gtgcccctca gtttcctgcc caccgaaaac tacgggatca gcggcatgga
     3601 cgaacaaact gcccgcaagg tgctatacaa caacggtttt atgtcgttcc ccacccatat
     3661 caccgacgcc agcggcaaca atacggccta tacccgtacc gaccgcatgc gccgcatttt
     3721 aggcgtcttc agcttttcaa agatggaacc gcacgttata cgcctcaaaa cggtagaacc
     3781 tgggcaatta tcgctgaatt ttgttgaata cattccaatc gaaatggttg ccgaagaagg
     3841 atacgattaa tcactgcact atgaaaaaaa cagtaaaaca tatcgcccgg tttttgcccc
     3901 tgctgttgct ggcggctgta gcctgccagg acagctggaa ccggcattac agctacgaca
     3961 aaaccctggg aggcgaagac ctgctgacca cgctcaaagc cgaagcttcc tacagccgct
     4021 ttgtaaaact catcgagatc gccggcctgc agaacaaact ggccggttcg ggctcttaca
     4081 cggtgtgggc tccgcacaac gattttattc ccgattcggt atatacctcg ggagatactg
     4141 ccctgatcag ggatgtcgtt cacaaccaca tcgcctattt cttgcattct actgccgaag
     4201 cctattcggg cagcagtgta atgatgctca acggaaaata catccacttc ggcaaagggg
     4261 gcagcgagaa attcgccttt gggggtttcg agctggatcc ggccgaccgg gtctgttcca
     4321 acggtgtttt gcacggactc gccggctatg tacctttcga atacaacctc tgggaatacc
     4381 tcgaaaaaac gcctgaactg agcctcttgc tgcaagccta caacttttac gattccacct
     4441 atatggaccg cgaacgcagc agacgcatag gtgtcgattc catgggcaac cacgtttacg
     4501 acacggtgtt gattcaggag aataccctgc tcaggcgcat cggaaaaatc tacaacgaag
     4561 atcggaacta caccatgatc gctcccacca accgggcctt tgaacaagcc ttcgattatt
     4621 acaaaaatta ctataaatcc aacggcggcc caacggtaac catgaataag gattccatac
     4681 agcgcacccg cacccacctg gctctgatca gaaatctgat cttcgagggc ggcttcgaaa
     4741 cactcaccga tacg
//
LOCUS       DAOSGL010000172         4743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16108_length_4743_cov_3.638865, whole genome shotgun sequence.
ACCESSION   DAOSGL010000172 DAOSGL010000000
VERSION     DAOSGL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16108_length_4743_cov_3.638865"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PKY68_06110"
     CDS             complement(<1..918)
                     /locus_tag="PKY68_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24644.1"
                     /translation="MKNLLLTAALALGVFVSCQQAGQESIDRKALVDRNKVQVNAFDS
                     LASLSLGNGNFAFTVDITGLQTFPERYSKGVPLGTQSQWGWHSFPNTRKYRFEETLRA
                     YNFRGKEELYAVQFNEKGRPQAAADYFRINPHRLHLGVVGLELRNPQGELMRPEDLSE
                     INQELDLWNGRINSLFKAGEFSVHTQSYCHPEMDMMASSIESSMFLSGDLRLRLAFPY
                     PSGNHSDDACDWNSPGKHSTKVLAQKPGVVLLERRLDNDSYYVLLCWKEQAEFSSGPE
                     AHHFYLQPVGEKLSFTCLFLDRNPLLDSSE"
     gene            complement(964..2406)
                     /locus_tag="PKY68_06115"
     CDS             complement(964..2406)
                     /locus_tag="PKY68_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOH24645.1"
                     /translation="MKTPNIISLVLTALLITACGPSQLQNGIPNFPWIAECGSQTQPP
                     VDKKFMANDYGALADTTQLSTEAVQRAIDACAEAGGGMVSFEPGSYCIGSIFLRSNVN
                     LNIPEGVVLLGSENILDYPLISTRVAGIEMEWPAALITAIDQDNVAVSGRGLVNGRGK
                     VFWDNYWEMRREYVPKGLRWIVDYDCKRPRLLLMQNCSNVTLSEAKFIEAGFWTVHIL
                     YSTQVTIKNLTIDNNNAGRGPSTDGIDIDSSNRILIEDCDISCNDDNFCLKAGRDADG
                     LRVNRPTEYVVIRNCIARYGDGLFTCGSETSGGIRNVVAYNLEAIGTKIGLRFKSAYT
                     RGGVVEDIYLANIKMRGLREPLQINLNWNPSYSYSSLPEAYDINEVPPHWKVMLTPVD
                     PPELGMPKFRNVHFTDIKADSCSVAITCEGAENSTIDQFYMNNVHITGAKAGRIYWAK
                     DWKLKNVWIGSEKGEMPDIQNTVNVPLLQE"
     gene            complement(2661..4049)
                     /locus_tag="PKY68_06120"
     CDS             complement(2661..4049)
                     /locus_tag="PKY68_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOH24646.1"
                     /translation="MKTKIFILCLLLGTSLTLQAKKTIKVACVGNSITQGAAIYNQQR
                     DSYPGLLGQMLGAGYEVRNYGFSGRTLLNKGDRPYMKEQMFLDALAFEPDIVTIKLGT
                     NDTKPFNWVHHHEFMRDLEQMVNAFSRLSSKPQIWLCYPVPAFHYDWGINDTIVRNQV
                     IPYIRKVAQKHDLNIIDLYTPFIGKGHLFADGIHPNEEGALVLAKEVYRALKGEEVPA
                     GYRPQAYPGVRSDWQGYERYNFNFKEKQATLVVPKQAAEGKPWIWRPAFFGAFPSVDL
                     ALLEKGYHVVYFELDNEFANPASLKAGDDFYKYMVKFYALSPRVVLEGFSRGGLYAFN
                     WALSYPQKVACLYLDAPVCDVYSWPGRERPEWQDFLKKWRLGEDQLGQFTANPVDNIE
                     ALVATQIPLILVAGTKDKLVPYKHNGAHLYQAYKKAGLEVKQILKKGVGHHPHSLEDP
                     AEIVEFILEHNK"
     gene            4207..>4743
                     /locus_tag="PKY68_06125"
     CDS             4207..>4743
                     /locus_tag="PKY68_06125"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOH24647.1"
                     /translation="MKKASFLIAFIALLLSSCCCKENALTVQVNNPSTLDRERENIEI
                     ELDAIKNGLGIKNDATSRFVVFDAQGQEIPSQLILSSTPATLLFQATIKAGDSCTFTI
                     KTGQPTPYESMVQTTFMPRRLDDMSWENDKVSFRAYGPALAKDPQQGMVSGGLDIWVK
                     RTSKLVTQQWYQDEFDGKS"
BASE COUNT         1182 a         1205 c         1185 g         1171 t
ORIGIN      
        1 ttcggaagaa tcgagcaaag gattacgatc gaggaaaagg caggtaaaac tcagtttttc
       61 gccgacgggt tgcaggtaaa aatgatgtgc ctcggggccg gagctaaatt cggcctgctc
      121 cttccaacac aacaggacgt aatagctgtc gttgtctaag cgacgctcca gcaagacaac
      181 tccgggcttc tgagccaaaa ctttggtact gtgtttgccg gggctgttcc aatcgcaggc
      241 gtcgtcgcta tggttgccgc tggggtaagg aaatgccagc ctcaggcgga ggtcgccgct
      301 aaggaacatg gacgattcta tggaagaagc catcatgtcc atctccgggt ggcaataaga
      361 ttgagtatgc acgctgaatt cgccggcttt gaataaggaa tttattctcc cgttccataa
      421 atcaagctcc tgattgattt cactcaaatc ttcgggccgc ataagctctc cctgaggatt
      481 gcgcaattcc aggcccacca cacccaaatg aaggcgatga ggattgatcc tgaaataatc
      541 ggcagcagcc tgcggacgac ctttttcgtt gaactgcacc gcatacaatt cttctttgcc
      601 tctgaaattg taggccctga gcgtttcttc gaaacggtat tttcgagtgt tggggaaact
      661 atgccagccc cattgcgact gagtgcccag aggcactccc ttgctgtatc gttcgggaaa
      721 agtctgcaaa ccggtaatgt cgacggtaaa agcgaaatta ccgttaccca acgataaaga
      781 ggccagggaa tcgaaagcgt tcacctgtac tttattcctg tcgaccaaag ctttgcgatc
      841 gatagactcc tgtcccgcct gctgacagga cacaaaaact ccgagcgcca gggcggcggt
      901 cagtaaaaga tttttcatgt ttggcatctg ttgaggtctg tgttttttat tctgttaaaa
      961 aaattattcc tggagcaagg gcacattgac ggtgttttgt atgtcgggca tctcgccttt
     1021 ctcgctgcct atccacacat ttttcaattt ccagtctttg gcccagtata tacgaccagc
     1081 tttggctccg gtgatatgta cattattcat gtaaaattga tcgatggtcg aattttcggc
     1141 tccttcacaa gtgatggcca ccgagcaact gtcggctttg atatcggtaa aatgcacatt
     1201 gcgaaatttg ggcataccca gttccggtgg atcgaccggg gtgagcatca ccttccagtg
     1261 cgggggcact tcattgatgt cgtaagcctc gggcaggctg gaatagctgt agctggggtt
     1321 ccagttgagg ttgatctgca aaggctccct gaggcctctc attttgatgt tggccaggta
     1381 aatatcttcg accacgccac cccgggtata ggccgactta aaacgcaagc ctatcttggt
     1441 gcctatggct tcgaggttgt aggccaccac gttgcggatg ccgcccgagg tttcgctgcc
     1501 gcaggtgaag aggccgtcgc cgtagcgggc aatgcaattg cggatcacca cgtattcggt
     1561 gggacggttc acccgcaggc cgtcggcatc gcggccggct ttgaggcaga aattgtcgtc
     1621 gttgcaactg atgtcgcaat cttcaatcag tatccgattg gaagaatcga tgtcgatacc
     1681 gtcggtactg ggacccctgc cggcattgtt gttgtcgatg gtcagattct tgatggtcac
     1741 ttgggtggaa tacaaaatat gcacagtcca gaaaccggct tctatgaatt tggcttcgct
     1801 cagcgtaaca ttgctgcaat tctgcatcaa caggagacgc ggccttttgc aatcgtaatc
     1861 gactatccag cgcaggcctt tgggcacgta ttcgcggcgc atttcccagt aattgtccca
     1921 gaacaccttg ccccggccat tcaccagtcc ccggcccgaa acggccacgt tgtcctgatc
     1981 gatggcggta atcagtgctg ccggccattc catttcgata ccggccaccc gggtagagat
     2041 caggggataa tccaggatgt tttcgctgcc caggagaaca accccttcgg gaatattcag
     2101 attgacattg cttctgagaa aaatggaacc tatgcaatag ctgccgggct cgaaactaac
     2161 catgcccccg ccggcttcgg cacaggcatc gatggcccgc tgaacggctt cggtgctgag
     2221 ctgcgttgta tcggcaagcg ctccgtaatc gttggccata aactttttat ctacaggagg
     2281 ttgggtttgt gaaccacatt cggctatcca gggaaaattg gggataccat tttgtaattg
     2341 agagggtccg caagccgtga ttaacagggc cgtcaggacc aggctgatga tgttgggtgt
     2401 tttcataact actaaaatta acggcagcaa caaagatatt tatttatccg ctaaagaaca
     2461 aaaaagcaag cgggctaaaa aattggtaga aaggctccgg caggaaaaag ctcccgggcg
     2521 agaaaggccc cgcaaaaaaa gctttccggc aggaaaaagc tctcagcaag aaaagtcccc
     2581 ggacagagaa actttccggc agaaaaaatg ccggcaggag aagtctatcg aaatggaagc
     2641 cccaataaaa gatcggcttt ttatttattg tgctccaaaa taaactccac tatctcggcc
     2701 ggatcttcca ggctgtgggg atgatgcccc acgccttttt tcagaatttg cttcacttcg
     2761 aggccggctt ttttataggc ctgatacaaa tgagctccgt tgtgcttgta aggcactaat
     2821 ttatctttgg tgccggctac cagaatcaaa ggaatctgcg ttgcaacgag ggcttctatg
     2881 ttgtccaccg ggttggctgt aaattgcccg agttgatctt cgcccagtcg ccattttttc
     2941 agaaagtcct gccattcggg acgctcccgg ccgggccaac tgtaaacatc acagacaggc
     3001 gcatccaggt aaaggcaggc cactttttga ggatagctca aagcccagtt aaaggcatac
     3061 aagcctccgc ggctgaagcc ttcgagcacg acccggggcg acaaggcgta aaatttgacc
     3121 atgtatttgt agaagtcatc gcctgccttg agcgaagccg ggttggcaaa ttcattgtcg
     3181 agctcaaaat aaaccacgtg atagcctttc tccaacaagg ccaggtcgac cgaaggaaag
     3241 gctccgaaaa aagccggccg ccagatccag ggcttacctt ctgcggcttg tttgggcacc
     3301 accagggtag cctgtttttc cttgaaattg aaattatagc gttcgtagcc ctgccagtcg
     3361 gagcgaacac cgggatacgc ctgcggacga tagccggccg gcacttcctc acctttaagg
     3421 gcacgataca cttctttggc caacaccaaa gctccctctt cgttgggatg gatgccgtcg
     3481 gcaaacaaat gtcctttacc gataaaaggc gtgtacaggt cgatgatgtt caagtcgtgt
     3541 ttttgggcca ccttgcgaat ataagggatc acctggttgc ggactatcgt atcgttgatg
     3601 ccccagtcgt agtggaaggc cggcacagga tagcacaacc agatttgcgg ttttgagctt
     3661 agccggctaa aagcattcac catttgttcc aggtcgcgca taaattcatg gtgatgcacc
     3721 cagttgaagg gtttggtatc gttggtaccc agtttgatgg taacaatgtc gggttcaaaa
     3781 gccagggcat cgagaaacat ctgctccttc atgtaggggc ggtcgccctt attgagcagg
     3841 gtgcggccgc tgaatccgta attgcgcact tcgtaaccgg ctccgagcat ctgccccagc
     3901 aaacccggat agctgtcgcg ctgctgattg taaatggctg ccccttgggt gatgctgttg
     3961 cccacacagg ccactttgat ggttttcttt gcctgcaggg tcagacttgt ccccaacaac
     4021 aagcacaaaa taaaaatttt agttttcatt agtatctctt acattaaacg gcgttttcgg
     4081 agagcaaaaa tacaatttaa ctgttaatac gctaacaaaa ctttgtctat ttaactactt
     4141 gtacctacat ttgtgcctca cattagagga acttagcaat ttatcacatt tttaacattt
     4201 catatcatga aaaaagcatc ttttcttatc gccttcatag cactcttgct cagctcttgt
     4261 tgttgcaaag aaaacgcttt gaccgtacaa gtaaataacc cttccactct ggacagagag
     4321 cgtgagaaca tagaaatcga gttggacgcc atcaaaaatg gcctgggaat aaaaaacgat
     4381 gccaccagcc gttttgttgt cttcgacgct caaggtcagg aaatccccag ccagcttatc
     4441 ctcagttcca caccggccac ccttcttttt caggccacga tcaaagccgg cgactcctgc
     4501 acttttacca ttaaaacagg acagcccacg ccctacgaaa gcatggtgca gaccaccttt
     4561 atgcctcgtc gcctcgacga tatgtcctgg gaaaacgaca aagtcagctt ccgagcctat
     4621 gggccggctc tggcaaaaga tccccaacaa ggtatggtaa gcggtggcct cgacatctgg
     4681 gtaaaacgca catcgaaact ggtcacccaa caatggtatc aggacgaatt cgacggtaaa
     4741 tcc
//
LOCUS       DAOSGL010000173         4738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16129_length_4738_cov_3.458894, whole genome shotgun sequence.
ACCESSION   DAOSGL010000173 DAOSGL010000000
VERSION     DAOSGL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16129_length_4738_cov_3.458894"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PKY68_06130"
     CDS             complement(<1..770)
                     /locus_tag="PKY68_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOH24648.1"
                     /translation="MNTNKFSKDVIEILGFSNEEARRLGNAVIDPEHLLLGILREKNS
                     KALICLNKLNVDVAFVKQSIEKQIKQEKMIQNPVDLPLSASTERVLKMGLLEAHGMKS
                     EIMDTEHILLAILKEKNNLAANVLNNEGVQWETLRSLFYEKKYPTSGPEFTDEEDDDT
                     EEYGTGGKPDIFQKTQDRPGTGRDTPALDNFGNDLTKAALEGRLDPIVGREKETLRLA
                     QILSRRKKNNPVLIGEPGVGKSAIVEGLALRIVQRKVSR"
     gene            1011..3542
                     /gene="gyrA"
                     /locus_tag="PKY68_06135"
     CDS             1011..3542
                     /gene="gyrA"
                     /locus_tag="PKY68_06135"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH24649.1"
                     /translation="MIDEERVIRVNIEEEMKSSYIDYSMSVIVSRALPDVRDGMKPVH
                     RRVLFGMNELGNNSDKPYKKSARIVGDVLGKYHPHGDSSVYLTMVRLAQDWSMRYPLV
                     DGQGNFGSVDGDSPAAMRYTECRLRKIAEEMLIDIDKDTVDFQQNFDDTLKEPTVLPT
                     RIPNLLINGASGIAVGMATNMPPHNLSEIVDGTIAYIDNNDISIDELMKYVKAPDFPT
                     GGFIYGYSGVKEAFETGRGRVVIRAKADIESGPQHDKIIITEIPYLVNKADLIKSIAD
                     LTIDKRIEGISNVNDESDRSGMRIVVDVKRDANSNVVLNKLFKMTALQSSFSVNNIAL
                     VYGRPRLLNLKDLIRYFVDHRHDVVNRRTQYELNEAEKRAHILQGLIIACDNIDRVIA
                     LIRASDSPDDARAALMEAFDLSEVQARAIVEMRLRQLTGLEVDKLHKEFDELTQKIAW
                     YKEVLANVSLRMNIIKEELLEVKNKYGDPRRSEIIYASEDFNPEDFYSDDQMIITISH
                     LGYIKRTPLADFRSQNRGGVGSRGSETRDEDFVEYIYPATMHNTMMFFTQKGKCYWLK
                     VYEIPEGSKNSKGRAIQNLLNIDAEDKVNAFIRVTSLDDKEFNDTHYLIFATKQGIIK
                     KTPLEAYSRPRNNGVNAITIREDDQVIQVRLTDGNSEIILANRNGRAIRFHESNVRPM
                     GRTATGVRGMRIDEDGQDEVIGMIIVSEDESIMVVSEQGYGKRSEVDDYRITNRGGKG
                     VKTLNITEKTGKLVAIKSVNDENDLMIINKSGIAIRLPLADVRIMGRATQGVRLINLE
                     RHSDEIASVCKVTSEKEEELEIEDQDNSDEEEFSTNQNEQTADEN"
     gene            3619..>4738
                     /locus_tag="PKY68_06140"
     CDS             3619..>4738
                     /locus_tag="PKY68_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH24650.1"
                     /translation="MKKIALSLTAILLLCGCNTYAQVKNVRKAKARLNAETPNLVEAR
                     DAIEPALSDSITKTMADTWFTAGKIYYKLFDQEQKKEWSGAKADGELMAQSLAKCYDS
                     FVIADSLDQIPDKKGRVKPKFTKSITEMVQAMQNGFINSGSFYFKKEDYQSAIRMFEY
                     YLDYPNLKFWTEEERLGYLKDTMIDDIQYYCGASASQDGDSETAIKYFTLLLDVYEPQ
                     EEMYQFLIYEYGRLKDSVNLLEMYKIGVQKFPQNPFYARSLINVYLNKNDLPEAMIWI
                     DKAIEEDSVNATLWDVKGRIFESEKNFEEAERCFLKAIEMDPDFEPALGNVGRIYYNR
                     AVEELDRVNAIRDDRVYRREKAKMKEVFEKPLPYMEKAH"
BASE COUNT         1399 a          944 c         1061 g         1334 t
ORIGIN      
        1 cgcgacacct tgcgttgtac tatgcgcagg gctaaacctt ctacaatggc cgatttccct
       61 acgccgggct ctccgataag taccggattg tttttctttc ttctactcag aatctgggcc
      121 agcctcaggg tttctttttc gcgcccgaca atgggatcga gcctgccttc caaagcagcc
      181 tttgtaagat cgttgccaaa attgtccaga gcaggagtgt ccctgccggt acccggtctg
      241 tcctgggttt tttgaaatat gtcaggtttg ccgccggtac cgtattcttc ggtgtcatcg
      301 tcttcttcgt cggtaaactc aggccccgaa gtagggtatt ttttttcata aaacaggctg
      361 cgcaaggtct cccattgtac tccttcgttg ttgagcacat tggctgccaa attgtttttt
      421 tcttttagaa tggccaaaag aatatgttct gtatccatta tttcactttt cattccgtgt
      481 gcctcgagca agcccatttt gagaactcgc tcggtagatg cgctcagagg taaatcaacg
      541 ggattctgaa tcatcttttc ctgcttgatc tgtttctcga tggattgctt cacaaaagcc
      601 acatccacat tgagtttgtt gaggcaaatc agggctttgc tgtttttttc cctcaatatt
      661 cccaacaaaa gatgctcagg atcaataaca gcattgccca acctgcgggc ttcttcgtta
      721 ctgaagccta aaatctcgat cacatctttg gaaaatttat ttgtattcat cgttataatc
      781 tttctgttca aaatgtaaaa cctaagataa acatttatac ggtcaaatgt acttgttttc
      841 tatcagtttt tccttactat gagcaaaaaa cttgccaaag gacaaaatgt cagtatagat
      901 tgtcttgaaa gtggcttgat tccttgaatt tattgttgtt ttttttgccc agttttttta
      961 aaagtgctac attagcgtgt ttttatttta actgttaaga tttttatcaa atgattgatg
     1021 aagaaagagt tataagggtt aacattgagg aagaaatgaa atcttcttac atagattatt
     1081 ctatgtcggt catagtttcc cgagccttgc ccgatgtacg tgacggtatg aagccggtac
     1141 atcgcagagt gctgtttggt atgaatgaat taggcaataa ttcagataaa ccttacaaga
     1201 aatcagccag aattgtgggt gatgtgcttg gtaaatacca tccccacgga gattcttctg
     1261 tttatttaac catggtacgt ctcgcccagg actggtctat gcgttatcct ttggtagacg
     1321 gacaaggcaa cttcggttcg gttgacggcg acagtccagc tgccatgcgt tacaccgaat
     1381 gtcgtttgcg caaaattgcc gaagaaatgt tgattgatat cgacaaagat accgtcgatt
     1441 ttcaacagaa ctttgacgat acgcttaagg aaccaacggt tttgcccact cgtattccca
     1501 acctacttat aaatggagcc tcaggtattg ccgtgggtat ggccaccaat atgccacctc
     1561 ataatttatc ggaaattgtg gacggaacta ttgcctatat cgataacaac gacatctcta
     1621 tagacgagtt gatgaaatat gtcaaggctc ccgatttccc caccggtggc tttatttatg
     1681 ggtattctgg cgtgaaggaa gcttttgaga caggaagagg ccgtgttgtg attcgcgcca
     1741 aggccgatat tgagagtggt ccgcaacacg ataaaataat tataaccgaa attccctatc
     1801 tggtcaacaa ggcagatctc atcaaaagca ttgccgacct aacaatagat aaaagaatcg
     1861 aaggtatttc gaatgttaac gacgagtctg atcgcagcgg tatgcgcatt gtcgtagatg
     1921 taaaacgcga tgccaattcc aatgttgtac tcaataaact gtttaagatg acggctttgc
     1981 agtcttcgtt cagtgttaat aacattgcgt tggtgtatgg gcgtccgcgt ttgttgaatc
     2041 ttaaggacct gattcgctat tttgtggacc acaggcacga tgtggtaaac cgccgaactc
     2101 aatacgaatt gaacgaagct gaaaaacggg ctcatatttt gcaaggcttg attatagctt
     2161 gcgacaatat tgaccgtgtc atcgccctta ttagagcatc tgattctccg gacgatgcca
     2221 gagctgcttt aatggaggct tttgatttga gcgaagtgca ggcacgggct atagttgaaa
     2281 tgaggctgcg tcagcttacc ggattggaag ttgataaatt gcacaaggaa tttgatgaat
     2341 taacccagaa aattgcctgg tacaaagagg tcttggccaa tgtgagtttg agaatgaata
     2401 tcatcaagga agaattgctg gaagtcaaga ataaatacgg cgatcccagg cgttcagaaa
     2461 tcatatacgc ttcggaagat ttcaatcccg aagatttcta ttctgacgat caaatgatta
     2521 tcactatttc acatctgggt tacataaaac ggacgccttt ggctgatttc aggtctcaaa
     2581 accggggcgg ggtaggttcc cgaggcagtg aaacccgcga tgaagacttc gtagaataca
     2641 tttatccggc caccatgcac aacaccatga tgttttttac tcaaaaaggt aagtgttact
     2701 ggcttaaagt gtatgagatt cccgagggtt ccaaaaattc caaaggccgg gctattcaaa
     2761 atctgcttaa cattgatgcc gaagacaaag tgaatgcttt catccgtgtg acaagccttg
     2821 acgacaaaga attcaacgat acgcactatc tgatctttgc caccaagcag ggtattatca
     2881 agaaaactcc gctggaagcc tattccagac cccggaacaa tggtgtaaat gcaataacca
     2941 tacgcgaaga cgatcaagtg attcaggttc gtctgaccga cggaaacagt gaaatcattc
     3001 tggcaaacag aaacggacgc gccattcgtt tccatgaaag caacgtcaga cccatgggcc
     3061 gcacggctac cggcgtacgc ggtatgcgta tcgatgaaga tggccaggac gaagttattg
     3121 gaatgattat tgtttcggaa gacgaaagca ttatggtggt ttccgaacaa ggctacggca
     3181 aacgttccga agtggatgat tatcgtatca ccaacagggg agggaagggt gttaaaactc
     3241 tgaatatcac tgaaaaaact ggtaagctag tggccattaa gtcggtaaat gatgaaaacg
     3301 atctgatgat catcaacaaa tccggtattg ccatcaggct tccattggcc gatgtccgta
     3361 tcatgggcag ggccactcag ggagttcgtc tgatcaattt ggaaaggcat agcgatgaga
     3421 tcgcttcagt ttgcaaagtt acttcggaaa aagaagaaga gcttgaaata gaagatcagg
     3481 ataattcaga cgaagaagaa ttttcaacaa atcaaaatga acagacggca gacgagaatt
     3541 aattcttttc tattgacaga atcaaattca gatgtatatt tgtatctaaa acatcaacaa
     3601 tattaaaaaa tgaatcatat gaaaaaaatt gctttatcac ttacagccat ccttttattg
     3661 tgtggatgta atacttatgc acaagttaaa aacgtgcgca aggccaaagc cagactgaat
     3721 gccgagacac ctaatcttgt cgaagccaga gatgccattg agcctgcatt gagcgattca
     3781 atcaccaaaa caatggctga tacctggttt acagcaggta agatctatta caaactgttc
     3841 gaccaggaac aaaagaaaga gtggagcggc gccaaagctg acggagaatt aatggcacag
     3901 tcactggcaa aatgttacga tagctttgtg atagccgatt ctttggatca aatacccgat
     3961 aagaagggca gagtgaagcc taagtttacc aaatccatta cggaaatggt acaagccatg
     4021 caaaatggtt ttattaattc cggctctttt tattttaaaa aggaggatta tcaaagtgcc
     4081 atcaggatgt tcgaatatta tttggattat ccgaatctga aattttggac agaggaagaa
     4141 aggctgggtt atctgaaaga caccatgatt gacgacatac agtattattg cggagcttca
     4201 gcttctcagg atggagactc tgaaacagcc atcaaatatt ttactttgct gttggatgtc
     4261 tatgagcccc aggaagaaat gtaccaattc ctgatttacg aatacggacg tctgaaagat
     4321 tcagttaact tgttggaaat gtataagata ggcgttcaaa agtttcctca aaatcctttt
     4381 tatgcccgta gtcttattaa cgtttattta aataagaacg atttgcccga agctatgatc
     4441 tggattgaca aagccatcga agaagactct gtaaatgcca ctttgtggga tgtgaaaggc
     4501 cggattttcg aaagtgaaaa gaattttgag gaagccgaac gctgtttttt gaaagctatt
     4561 gagatggatc ctgattttga accggctttg gggaatgtcg gccgtattta ttacaacaga
     4621 gccgtcgaag aacttgaccg tgtaaatgcc attagagacg acagagttta tcgtcgcgaa
     4681 aaagctaaaa tgaaagaagt atttgaaaaa cctttgcctt atatggaaaa agctcacg
//
LOCUS       DAOSGL010000174         4724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16184_length_4724_cov_3.968730, whole genome shotgun sequence.
ACCESSION   DAOSGL010000174 DAOSGL010000000
VERSION     DAOSGL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16184_length_4724_cov_3.968730"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKY68_06145"
     CDS             <1..541
                     /locus_tag="PKY68_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH24651.1"
                     /translation="YNATARKITGIKLHDMNCGLKAYRKDVVKNIEVYGEMHRYIPYL
                     AKNAGYTKIGEKVVRHQKRKFGESKFGLSRFAHGYLDLMTLWFLSHFGKRPMHFFGLV
                     GSLLFLLGFIAVVVLGAMKISMLNQGIPFRLITDISYFYLALTCILLGTQLFLAGFLA
                     EMISRNASERNNYIIDQEL"
     gene            564..998
                     /locus_tag="PKY68_06150"
     CDS             564..998
                     /locus_tag="PKY68_06150"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HOH24652.1"
                     /translation="MSLFLQACVGNPNCYESKEVLVYCGFYSSETGKAGSIEKVSVRG
                     LGSDSLIYNDKTLSKLALPLRSDAQESVFIIEIEQSSIVFRDSLSLSHTNNEWFISME
                     CGCIVLHTLLDARPGGASFIDSVCIVNPEIKNAVTEHVKIYL"
     gene            979..1668
                     /locus_tag="PKY68_06155"
     CDS             979..1668
                     /locus_tag="PKY68_06155"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HOH24653.1"
                     /translation="MSKYIYSLLIFSMINLTTLAQTRPKAARDSVFYHGAYLGISLIE
                     PVKSFFSATWGSSVQLDVNLLNRFFPVVELGYAQAKLVNPEQTRFDTKAIYYKAGINL
                     PIAKEGPNAENLFFLGGRLGYCSFDYSISGCVFDGSYWNRGYMKDFLNEHAGAAWGEV
                     LAGLRVQLFGPISLGWSGRMKTTFKVRNGEHSVPPYIPGYGLNQKPNYDLLLHLYFRL
                     PGSGTKTNKRL"
     gene            1829..2833
                     /locus_tag="PKY68_06160"
     CDS             1829..2833
                     /locus_tag="PKY68_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOH24654.1"
                     /translation="MKTRLFFPILLLMLVSCKDKTVFQRNQGFIFNTSYHISYTSNKD
                     LHAGIRQELEAFDASLSMFNPESTISRINRSDTGAIDLSGDPRVVYLLNEAIKISEIT
                     EGAFDITVAPLVNAWGFGTEQRKSMSSVDIDSLMQFVGYEKLQLEGFKLSKADARVKL
                     DASAIAKGYACDIIAEKLEQAGIKDYLVEIGGEIRMAGLNPKGQLWKVGIDKPIDDSL
                     AVERELQAALALTGKGMATSGNYRNFYKIQNQKLGHTIDPHTGKPIQQNILSATVVAS
                     DALTADAYATAFMVMGLEKARTLIDKLPGLEAYFIYSDDEGKLQTWISKGLQDALTTF
                     "
     gene            complement(2903..3883)
                     /gene="pfkA"
                     /locus_tag="PKY68_06165"
     CDS             complement(2903..3883)
                     /gene="pfkA"
                     /locus_tag="PKY68_06165"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOH24655.1"
                     /translation="MALIKTIGVLTSGGDAPGMNAALRAVTRSAIFNGLRVKAIYRGF
                     RGLLTGEIAEFKTQNVSNIIQQGGTVIKTARCKEFQTPEGRRQAYERIKKEGIDAMVV
                     IGGDGTLTGARIFASEFNFPIVGVPCTIDNDIYGTDSTVGYDTALNTILDAVDKIRDT
                     ASSHERLFFIEVMGRDAGFLALNGAIASGAEAAIIPETTTEVDQLAEFVKSGFRKSKN
                     SSIVLVAESDVTGGAMHYADRVKNEYPDYDVRVVILGHVQRGGSPSAYDRILASRMGN
                     AAVEALIDGQRNIMMGIDDDQIVAVPLSRAIKNDKPLDRELLKVLRTLSI"
     gene            complement(3934..4533)
                     /locus_tag="PKY68_06170"
     CDS             complement(3934..4533)
                     /locus_tag="PKY68_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HOH24656.1"
                     /translation="MITDKFPYKLILASNSSRRRDLLAGLGLPFEFRTPPESDENYPP
                     DLRGEEIPLFLARAKAEACRTLINAGELFITADTIVWHQNKVFGKPRHHEEAVEMLRA
                     LSGCTHQVFTAVCLTTQQAQRSFCSSSEVRFCRLEEEEIRWYVEHYSPLDKAGAYGIQ
                     EWIGYVGVEHISGSYFNVMGLPVHRLYRELLAFASQAAG"
     gene            complement(4535..>4724)
                     /locus_tag="PKY68_06175"
     CDS             complement(4535..>4724)
                     /locus_tag="PKY68_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HOH24657.1"
                     /translation="IPDYEVMKQVGLPCCPADAAPEIRAIARYVSAYKGGEGCGRDLL
                     EQILKVQGHWMSEGAFGW"
BASE COUNT         1206 a         1181 c         1126 g         1211 t
ORIGIN      
        1 atacaatgcc actgcccgta agattaccgg tattaagctg cacgacatga attgcgggct
       61 gaaagcctat cgcaaggatg tggtaaaaaa catcgaagta tacggcgaaa tgcaccgcta
      121 catcccctat cttgccaaaa atgccggcta tactaaaata ggcgaaaaag tggtgcggca
      181 tcagaagcgt aaattcggag agtcaaaatt cggtctgagc cgttttgccc acggctacct
      241 cgacctgatg accctttggt ttttatcgca tttcggcaag cggcccatgc acttttttgg
      301 cctggtaggc agtttgctct ttttgttggg ttttattgcc gtcgtggtgc ttggagcgat
      361 gaaaatttcc atgctcaatc aaggcattcc cttccggctg attaccgata tctcttattt
      421 ctatttggcc ctaacctgta ttttgctggg aacccagttg ttcctggccg gtttcctggc
      481 cgaaatgatt tcacgcaatg cttcggaacg caacaactac atcatcgatc aggaacttta
      541 gcttgctact cacattgctg cttttgtcgc tttttttaca agcctgtgtc ggcaacccca
      601 actgctacga atcgaaagaa gtattggtct attgcggctt ttactccagc gagaccggca
      661 aagccggcag catcgaaaaa gtcagtgtta ggggtctggg cagcgattct ctgatttata
      721 acgacaaaac gctctcgaaa ctggcccttc cgctgcgttc cgatgcacag gaaagcgttt
      781 ttattatcga aattgaacaa tcttccattg tgtttcgcga cagcctcagt ttatcgcaca
      841 ccaacaacga atggttcatt tctatggaat gtggttgtat agtgttgcat accctgttag
      901 atgcccggcc gggcggagca agctttatcg attcggtctg catcgtgaac ccggaaatca
      961 agaatgcggt tacagaacat gtcaaaatat atttatagtc tcctgatttt cagcatgatc
     1021 aaccttacaa ccttggcgca aaccagaccc aaggcagcca gggattccgt cttttatcac
     1081 ggtgcttatt tgggtatcag ccttatagag ccggttaaga gttttttttc tgccacttgg
     1141 ggaagctccg tgcaattgga tgttaattta ctgaaccgtt ttttcccggt cgttgaactg
     1201 ggctacgccc aggcaaaact tgtgaacccg gagcagaccc gtttcgacac caaggccatc
     1261 tattacaaag cgggaatcaa cctgcctata gccaaggaag gccccaacgc cgaaaatctg
     1321 ttttttttgg gtggccggct gggatattgt tcttttgatt attccataag cggttgcgtt
     1381 ttcgacggta gctattggaa cagaggctat atgaaggact tcctcaacga acatgccgga
     1441 gctgcatggg gagaagtgct ggcaggcctt cgtgttcaac ttttcgggcc aatatcgcta
     1501 ggctggtcgg gcagaatgaa aaccaccttc aaggttcgga acggagagca ttctgttcct
     1561 ccttatattc cgggctacgg actcaatcaa aagcccaact acgatttact gttgcatctt
     1621 tacttccgtc tgcccggttc cgggacaaaa acaaacaaac ggctctgaga cttggtgtca
     1681 gcttaattat gtttagtatg aaagagaagc tcgcaggcag gcccaggagg ccacaaacgg
     1741 tgtcagttta attatattta gtatgaaagc cgttggatat cgcttttttc acgtttacag
     1801 tataatgcac ccgtctaatt ttattaatat gaaaaccagg ttgtttttcc cgattctgtt
     1861 gctgatgctc gtttcctgca aagacaaaac agtctttcag cgcaaccagg gctttatttt
     1921 caataccagc taccacatca gctacacctc gaacaaagat ttacatgccg gtatcagaca
     1981 ggaactggaa gccttcgatg cttccctgtc aatgttcaat cccgaatcca ccatcagccg
     2041 cattaaccgc agcgataccg gagctatcga tctttcgggc gatccacggg tggtttatct
     2101 gctgaacgag gctataaaaa tttcagaaat aacggaggga gctttcgata taacagtagc
     2161 tccgctagtc aatgcctggg gctttggcac cgaacaacgc aaatcaatga gcagcgtcga
     2221 catagacagc ctgatgcaat ttgtgggcta tgaaaaattg cagcttgagg gtttcaaact
     2281 gagcaaggcc gatgccaggg tcaaactgga tgcttcagct atagctaagg gttacgcctg
     2341 cgatattatt gccgaaaagc tggaacaagc cggtatcaaa gactatttgg tcgaaatcgg
     2401 aggcgagata cgcatggcag gtttgaatcc caaggggcaa ttgtggaagg tggggataga
     2461 caaacctata gacgactcgc tggctgtaga aagggaattg caagcagctc ttgccttaac
     2521 cggcaaaggc atggcaactt cgggcaatta ccggaatttc tacaaaatcc aaaatcagaa
     2581 actgggtcat accatagatc cccacacggg aaaacccatt caacagaata tactcagcgc
     2641 caccgtcgta gcttcagacg ccctaacggc agatgcctat gccacggcat ttatggtgat
     2701 gggactggaa aaagcccgca ctttgatcga caagctgccc gggctggaag cctattttat
     2761 ttattccgac gacgaaggca aactgcaaac ctggatcagc aaaggtttgc aggacgcctt
     2821 gactactttc tgagccgatc caataaaaca ctgaccggac cagaagggaa aaattcagca
     2881 tctttactgt ttctaaatct actcagatgg aaagagtcct gagtactttg agcaattctc
     2941 ggtccagggg cttatcgttt ttgatggctc tggagagcgg aactgccact atctgatcgt
     3001 cgtcaatgcc catcataata ttgcgctggc cgtcgatgag ggcctctacg gcagcattac
     3061 ccatgcggct ggccagaata cggtcgtaag ctgagggaga tcctccgcgt tgcacgtgac
     3121 ccagaataac cacccgcaca tcgtaatcgg ggtactcgtt tttaacccgg tcggcgtaat
     3181 gcatggcgcc cccggtaaca tcgctctcgg ccaccagcac tatactgctg ttttttgatt
     3241 tgcgaaagcc cgactttacg aattccgcca gctgatccac ttcggtggta gtttcgggaa
     3301 taatggcggc ctctgctccc gaggcgatgg ctccgttcag ggccagaaaa ccggcatcgc
     3361 gtcccatcac ttcgataaaa aacagacgtt cgtgcgaaga ggctgtgtcg cgaatcttgt
     3421 ccacggcatc cagtatggtg ttcaaggcgg tatcgtaacc tacggtactg tcggtaccgt
     3481 aaatatcgtt gtctatggta cagggtacgc ctacgatggg gaaattaaac tcggaagcaa
     3541 aaatccgggc tcccgtcagt gtgccgtctc ctccgataac aaccatggcg tcaatccctt
     3601 ctttctttat gcgctcgtaa gcctgacgac ggccttcggg agtttgaaat tccttacaac
     3661 gggcggtttt aataacagta cctccctgtt gtatgatatt gctgacgttt tgggttttga
     3721 attcagcgat ttcgccggtt agtaggcctc taaagccccg gtatattgcc tttaccctca
     3781 atccgttgaa aatagccgaa cgggtaacgg ccctcagtgc ggcgttcatg ccgggagcgt
     3841 ctcctcccga agtcaacacg cctattgttt tgatcaatgc catattatat tctttaaaaa
     3901 cgggggcaaa gataatacat ttttgtcatt cagtcatccg gccgcctgag aagcaaaggc
     3961 cagaagttcc cggtataaac gatgcaccgg taaacccatt acgttgaagt atgaaccact
     4021 tatgtgttcc acacccacat agcctatcca ttcttgaatg ccgtaagctc cggctttatc
     4081 gagtggagaa taatgctcca cataccaacg gatttcctct tcttccaggc gacaaaaacg
     4141 tacttcggac gaagaacaga agctgcgctg agcctgctga gtcgtaaggc aaacagccgt
     4201 aaatacctga tgggtgcatc cggacaaggc tcgcagcatt tctacggctt cttcgtggtg
     4261 gcggggtttg ccaaatactt tattctgatg ccagacaatc gtatcggccg ttataaacaa
     4321 ttcaccggca tttatcaagg tacgacaggc ttcggccttg gcacgggcaa gaaacaaagg
     4381 aatttcctcg ccccgaagat ccggaggata gttttcgtcg ctttcgggcg gtgttctgaa
     4441 ctcaaagggc agacccaacc cggctaacaa atcccgcctt ctggaagaat ttgaggccaa
     4501 tatcagttta taagggaact tatctgtaat catcttacca gccaaaagca ccctcactca
     4561 tccaatggcc ctgcactttg aggatttgct ccagcagatc acggccgcaa ccctctccac
     4621 ccttgtaggc agaaacataa cgcgctattg ctctgatttc gggggcggcg tcggccggac
     4681 agcaaggcag accaacctgc ttcatcactt cgtaatcggg gatg
//
LOCUS       DAOSGL010000175         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16258_length_4710_cov_4.273469, whole genome shotgun sequence.
ACCESSION   DAOSGL010000175 DAOSGL010000000
VERSION     DAOSGL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16258_length_4710_cov_4.273469"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2464)
                     /locus_tag="PKY68_06180"
     CDS             complement(<1..2464)
                     /locus_tag="PKY68_06180"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOH24658.1"
                     /translation="MELPSKYNPAEVESKWYQYWLQHNMFHSTPDEREPYTIVIPPPN
                     VTGVLHMGHILNNTIQDILIRRARMLGKNACWVPGTDHASIATEAKIVEKLKNEGIKK
                     TDIGREAFIEHAWEWTHKHGGIILEQLKKLGASCDWDRTCFTMDDARSESVLKVFIDL
                     FEKGLIYRGVRMINWDPKAQTALSDEEVIYKEVQSKLYYLRYKVQGEDRYATIATTRP
                     ETILGDTAVCINPKDESKAWLRGKKVLVPLIDRPVPIIEDEYVDIEFGTGCLKVTPAH
                     DVNDYMLGEKYRLESIDIFNNDGTLNKHGAMYAGMDRFEVRSKIEKDLKAAGLLEKVE
                     DYTNKVGYSERTDEAIEPKLSMQWFLKMSDLAKPALEAVEKDEIKLIPPKYKNTYRYW
                     MENVKDWCISRQLWWGHRIPAYYLPEGGFVVASTPEEALEKANALRMKNACPCGCSGL
                     KPLKPEDLRQDEDCLDTWFSSWLWPISVFDGINEPDNKDINYYYPTNDLVTAPDILFF
                     WVARMIISGFEYRKEACFKNVYLTGIVRDKLGRKMSKSLGNSPDPIELMDKYGADGVR
                     MGMMLAAPAGNDVLFDEALCEQGRNFNNKIWNAYRLISGWKVDPKLDQPKASALAVEW
                     FEARLGKTLAEMDDLFDKYRLSEAMMCVYKLFWDEFSSWFLEIIKPAYRQPIDGITHR
                     AVFGFFEQLCLLLHPFMPFITEELWQHLEQREPGESLMLQQQRKPTAYDQVILDKFEL
                     VKAVVSGIRTIRLEKNLPVKTALALEIQGEHDASFDSLIEKMANVSSITQVNVKASDA
                     SSFLVNATEYSLLLGALIDTEGE"
     gene            2678..3364
                     /locus_tag="PKY68_06185"
     CDS             2678..3364
                     /locus_tag="PKY68_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24659.1"
                     /translation="MIQFNFMIIAHLKYYRGITALVLFCFFTACTKVGGFTIDVQGPD
                     IEFKMAFFDADNQREALKMASSGDHKSNQDAFWDYRPIAKSDTIYALDIEAFLSEVGQ
                     TFNSVVEKAELKNSKMRLIQDTVDFSGVNSFLIKVRKLGEEEFVPFCSTEINKQSNSK
                     QLSVESVYFEPNEVFKLIECGFEAILYAKINKDSQKPACLRDGIHFEFLSGTVIAVKV
                     KAGALFDLDI"
     gene            3371..4156
                     /gene="mazG"
                     /locus_tag="PKY68_06190"
     CDS             3371..4156
                     /gene="mazG"
                     /locus_tag="PKY68_06190"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOH24660.1"
                     /translation="MHDRQSKMEAFGRLLDILDQLRLKCPWDKEQTFESLRPLTLEEC
                     YELSEALLQKDHPDVCKELGDILLHIVFYSKIAEEQQLFDVKDVCDQLAEKLIFRHPH
                     VFGTVSVQDAGEVEKNWEKLKLKEKNGNGSVLAGVPSSLPSLIKAYRMQDKASGVGFD
                     WEDRRQVWAKVQEEIREFQREIEKDDKDGMEDEFGDLLFSLINVARLYGINPDNALEH
                     TNKKFRERFNYLEAETLKKGLLLKEMSLDQMEVIWQQAKKHIP"
     gene            4162..>4710
                     /locus_tag="PKY68_06195"
     CDS             4162..>4710
                     /locus_tag="PKY68_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH24661.1"
                     /translation="MNLNPAFVRQMRRLLPGESGAFMRSLEEDLPVSLRLNTAKCSGE
                     KALNCLPANVQSAVPWCPDAYYLEQRPFFTFDPFFHCGLYYVQEASSMFTARAIHAAL
                     QAMKMSGRAVRMLDLCAAPGGKSTLAASVLPEGSLLLANEIVPSRAVILEENLVKWGK
                     AEVVVTQNEPADFAAFEYTFLDI"
BASE COUNT         1238 a         1010 c         1113 g         1349 t
ORIGIN      
        1 gctctccttc cgtatcgatc aaagcgccaa gtaacagact gtattctgtt gcatttacca
       61 ggaaagagga cgcatcggaa gccttaacat tcacttgggt aatgctgctt acattggcca
      121 ttttttctat aaggctgtca aaacttgcat cgtgttcacc ctgtatttca agcgccagag
      181 ctgttttgac gggaaggttt ttttccaggc gaatggtacg aatgccagaa acaacagctt
      241 tgaccagttc aaatttgtcc aatattacct gatcgtaggc ggtcggcttc ctttgttgtt
      301 gtaacatcag gctttcaccg ggttctcgtt gttccagatg ctgccaaagt tcttcagtaa
      361 taaagggcat aaagggatgc aacaagagac agagttgttc gaaaaaaccg aaaacagccc
      421 tatgagttat gccgtcgatc ggctgccgat aggcgggttt aatgatttca agaaaccagg
      481 aagaaaattc gtcccaaaag agtttgtaaa cacacatcat ggcttccgac aagcggtatt
      541 tgtcgaacaa atcatccatt tcggccagtg ttttccctaa tcgtgcttcg aaccattcta
      601 cggccagagc agaggctttc ggttgatcta gtttggggtc taccttccag ccgcttataa
      661 gccggtaggc attccaaatt ttattgttga agttgcgacc ctgttcgcac aaggcttcat
      721 caaaaagcac atcgttgccg gcgggggcag ccagcatcat ccccatacgg acaccgtcgg
      781 caccgtattt atccatcaat tcgatgggat cgggtgagtt gcccagcgat ttggacattt
      841 tgcgtcccag tttgtcgcgg actattccgg tgaggtacac atttttaaaa caagcctctt
      901 tacggtattc aaagccggag ataatcatac gggccaccca gaaaaacaaa atatcgggag
      961 ctgtcaccaa atcgttggtg gggtagtagt aattgatgtc tttgttgtcg ggctcattta
     1021 ttccgtcaaa aacggaaatg ggccatagcc aggaggaaaa ccaggtgtcc agacaatctt
     1081 cgtcctgacg caggtcttcg ggtttgagag gcttcaatcc gctacagccg caggggcagg
     1141 catttttcat gcgcagcgcg ttagccttct cgagagcctc ttcgggagtg gatgctacta
     1201 caaaacctcc ttcgggcagg taataagccg gaatgcgatg tccccaccag agttggcggg
     1261 aaatacacca gtctttgacg ttttccatcc aataccggta ggtgtttttg tattttggcg
     1321 ggataagttt tatttcgtct ttttcaacgg cttctaaggc cggtttagcc aaatcggaca
     1381 ttttcaggaa ccattgcatc gaaagcttag gctctatggc ttcgtcggtt cgttcggaat
     1441 agcctacttt attggtatag tcttctactt tttcgagcaa tccggctgct ttaagatctt
     1501 tttcaatttt agatcttacc tcgaagcgat ccatgccggc atacatggct ccgtgtttgt
     1561 taagcgtacc gtcgttattg aaaatatcta tagattccag acggtatttt tcgcccagca
     1621 tgtagtcgtt cacatcgtga gccggagtca ctttgaggca gccggtaccg aattctatat
     1681 cgacgtattc gtcttcgatg atgggtacag gccggtcgat tagcgggacc agtacttttt
     1741 tacctctgag ccaggcctta ctttcgtcct tgggattgat gcagacagcc gtatcgccca
     1801 atatagtttc cggtctggtg gtggctatag ttgcgtaacg atcttcaccc tgcactttgt
     1861 aacgcaggta atagagtttg gactgtactt ctttgtaaat aacttcttcg tccgacagag
     1921 ctgtttgggc tttagggtcc cagttgatca tgcgtacacc tctgtaaatg agccctttct
     1981 caaaaagatc gataaaaacc tttaatacgc tttcagaacg ggcatcgtcc atggtaaaac
     2041 aggtgcgatc ccaatcgcag gaagctccca gttttttgag ttgttcaagt ataatacctc
     2101 catgtttgtg tgtccattcc caggcatgtt caataaaagc ctcccggccg atgtcggttt
     2161 ttttgatgcc ttcgtttttg agtttttcaa ctattttggc ttcggtggcg atggaagcat
     2221 gatcggttcc aggcacccag caagcatttt taccaagcat tcgggccctg cgaatcaata
     2281 tatcctggat ggtgttgttc agaatatgtc ccatatgaag cactcccgtc acattgggcg
     2341 gcggaataac aatggtatag ggttctcttt cgtcgggagt cgaatgaaac atgttgtgtt
     2401 gcagccaata ttgataccat ttactttcta cctcagcagg attgtactta gaagggagtt
     2461 ccataatagt tagaaatctt gtttggttaa atgccccggc ccgtgtttat cggattcagg
     2521 cactgtacag attttaaggc tgcaaaggta ggactatttt tttctatgcc caagtaattt
     2581 ttattacttt tagtgggcaa tccgggcaag gggtgatttg ttaatattgt ataacaatta
     2641 gcgtaaggaa tgtaacaagt tgtacatttg tattgaaatg attcaattta actttatgat
     2701 aatagcacat ctgaagtact atagaggaat aactgctctt gtattgtttt gtttttttac
     2761 agcctgcacc aaggtgggtg gatttactat tgatgttcag gggccggata ttgaatttaa
     2821 gatggccttt tttgacgctg ataatcagcg agaagcattg aaaatggcat catccggaga
     2881 tcacaaatcc aatcaggatg ctttctggga ttacagaccc attgcgaaaa gcgataccat
     2941 ctatgctctc gatattgagg cctttttatc ggaagtgggg caaactttca attctgtggt
     3001 tgaaaaggct gaattgaaaa attctaaaat gcgcttaatt caggatacgg ttgattttag
     3061 cggtgtgaat tcttttttga ttaaagtacg caagcttggc gaagaagagt ttgttccctt
     3121 ttgttcaaca gaaataaata agcagtcaaa cagcaaacaa cttagtgtag aaagtgttta
     3181 ttttgagcct aacgaggtgt ttaaacttat agaatgtggt tttgaagcca ttttatacgc
     3241 aaagatcaac aaagatagcc aaaaaccggc ctgtcttcgc gacgggatac attttgaatt
     3301 tctgtccgga accgtcatcg ctgtcaaagt aaaagccggt gcgctcttcg atctcgacat
     3361 ttaacctttt atgcacgaca gacaatcaaa aatggaagct ttcgggcgat tgctcgacat
     3421 tttggaccaa ttacgtttga aatgcccctg ggacaaagag caaaccttcg aaagcctgcg
     3481 ccctcttacg ctggaagaat gctacgaatt aagcgaagcc ctgttgcaaa aagaccatcc
     3541 cgatgtttgt aaagaactgg gtgatatcct tttgcatata gttttttatt caaaaatagc
     3601 cgaggaacag caattgtttg atgtcaagga tgtatgtgat cagctcgcag aaaagctgat
     3661 tttcaggcat cctcatgttt ttggtacagt cagcgtacaa gacgccggcg aggtcgaaaa
     3721 aaactgggaa aaattaaaac tcaaagaaaa gaacggcaat ggttcagtat tggccggagt
     3781 gccttcttcc ttaccctcgc ttataaaagc atacagaatg caggataaag cctctggagt
     3841 gggctttgac tgggaagatc gcagacaggt atgggctaaa gtacaggagg aaatcaggga
     3901 gtttcaacga gagatagaaa aagacgacaa agacggcatg gaagacgaat tcggcgattt
     3961 acttttcagc ctgattaatg tagcccgcct ctacggaatc aatcccgaca acgccctgga
     4021 acacaccaat aaaaagtttc gtgaacgctt taattacctg gaggctgaaa cactaaaaaa
     4081 aggcctgttg cttaaggaaa tgagtttgga ccagatggaa gtcatctggc aacaggcaaa
     4141 aaagcacatt ccctgaacaa aatgaattta aatcccgctt ttgtcaggca gatgcgccgg
     4201 ctattgcccg gtgaatccgg ggcgtttatg cgctctttgg aagaagatct tccggtcagt
     4261 ttgagattaa atacggccaa atgctccgga gagaaagcgc ttaactgctt gcctgcaaat
     4321 gttcagagtg ctgtgccctg gtgtcccgac gcctattatt tagaacaacg tccattcttt
     4381 actttcgatc cattttttca ttgcggcctg tattacgttc aggaagcatc ctctatgttt
     4441 accgcccggg ccattcatgc tgctttgcag gcgatgaaga tgtccggccg ggcagtgcgg
     4501 atgctggatc tttgtgcagc gccgggaggt aaatccacct tggctgcctc ggtgcttcct
     4561 gagggtagcc tgttgctggc caacgaaatc gttccttcga gggctgtaat actggaagaa
     4621 aacctcgtaa aatggggtaa ggcagaggtt gtcgttacgc aaaacgaacc ggccgatttt
     4681 gccgcatttg aatatacttt cttggacatt
//
LOCUS       DAOSGL010000176         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16685_length_4616_cov_4.261127, whole genome shotgun sequence.
ACCESSION   DAOSGL010000176 DAOSGL010000000
VERSION     DAOSGL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16685_length_4616_cov_4.261127"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PKY68_06200"
     CDS             complement(<1..1010)
                     /locus_tag="PKY68_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOH24662.1"
                     /translation="MNNEMRSFSLNLKIVLLLIFAGQSIGGSAQVIEPESDKDYYLIH
                     SGACVLSRADDGRAVVQTAGAIGTQVLNFVADGRGYYTVKLKDRREYMALSGSWNTYF
                     VSDSASDAAKYAVEEVRKGIVRLRCKANRKYLGTDNSTAGSHVYSDKSGLDERHYWLI
                     SEEIREFPVDTDSLYYLINPSDAFGKTFEGWGVSLCWWANMCGRWEENKIDEIIDWLV
                     SPEGLNYRIFRYNIGGGDDPLNRNCDPHHMALGKGLRAEMEGFKDSADAPYDWSRDAA
                     QRKIMLKIREKRPDAVFEAFSNSPPWYMTYSGCVAGNEPAGLDNLKPEYYEAFATYLV
                     DV"
     gene            complement(1104..3041)
                     /locus_tag="PKY68_06205"
     CDS             complement(1104..3041)
                     /locus_tag="PKY68_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="silent information regulator protein Sir2"
                     /protein_id="HOH24663.1"
                     /translation="MKTKSTLVRFALGGGATLLFLFAGLFKTGAQDPRERYYYYEILE
                     PHHAPKPQVEGFATMRVNEALDRGLTATRAQDGQSLYLSWRLLAEDPQDVAFDVIRVE
                     GQKNQRLNKRPITATCDFIVENPPAEAVYKVVPRNNRKSVNSAGAKPAANPGSAAASL
                     DKTKGVLNTGEGSISIRMEDLPAFPYRSIKLRDGVRPGKMALADLNGDGVYDYIIRTP
                     DSNVDPGMPGDLTGRTYQIEAYLSDGTFLWSFDLGQGIEPGVWYSPFICYDFNGDGKA
                     EVALKTAPGDIVRNEKGRVDYGDEYLSVLDGMTGREIDRVDWPVRNFRYGDLNRQSRH
                     QIGMAFLDGKTPYILAARGTYKLMTVDAWMLRGDKLELAWRWDGDEENPVVRSMGAHN
                     MICTDVDGDGRDEVVLGSCALDDNGTLLWSTGLGHPDKIFISDIDPNRPGMEMLLCLE
                     PWHDNGRGVCMIDPKDGSPIWNIGHKTYHVGDGMIADFDPSSPGLECFASEDQKGGSS
                     DKYLLSADGRIIGKNEEVPPCRNWAWWDADLLREYISAQFYRSPEGRFGRKSGIGKWK
                     GETLFGEMEGSILVIADVFGDWREEIITALPGELRVYTTNIPARDRRITLMQDALYRN
                     YVAHRSMGYDQAPVPSFYLGE"
     gene            3171..>4616
                     /locus_tag="PKY68_06210"
     CDS             3171..>4616
                     /locus_tag="PKY68_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HOH24664.1"
                     /translation="MNKLIFSLGLLFFTCPVSDAQGTFQHRATGSTTQTDRRFEGTAV
                     HTRAGAESLPATNPIHPQPVADRIVPFNLSDSGKHTPILWGLDTAWPSRDNIVRGVAF
                     MGSENIYLVRASFQPTMPLVNGDLQSQQVADLNNRLNLINLTGSHTKVALNCDHPSVD
                     PWYAGNAQNWAQLMDVTARRIQERGRRIVSIAPFNEPDYGWGQGSLTDFFNIAGELRN
                     NPRFDSIRISGGNTLNTDQALSWYNSLKSRLDEGNTHQLAGSFDNYASFFRTVRANGH
                     HATNDELHNVMEAMVGVEYGMQTGIWWGTAELARGEFVKASFGRRLAYAEHRPNWTAA
                     TVYRSPEGKVQAFVGSSERQAATTSYSFISFERDVFYDGQGPQRAFTIEIPGGTGYQQ
                     GQTNAERVININWGEDVPQPVNGRYVLVNRNSAKVMEVENGSTSAGSNVRQNTYTGAA
                     YQQWVVTPVDPRIGGDFSYHSIIANHSGKSLD"
BASE COUNT         1127 a         1288 c         1110 g         1091 t
ORIGIN      
        1 cagacatcta ctaaatacgt tgcgaaagcc tcataatatt ctggtttgag gttgtcgagg
       61 ccggccggct cgttacccgc cacgcatccg ctgtaggtca tataccaagg cggggaattg
      121 ctgaaagctt cgaatactgc atccggacgt ttttccctga ttttgagcat tattttgcgc
      181 tgggctgcat cgcgactcca gtcgtaggga gcgtcggcag aatctttgaa cccttccatc
      241 tcggcacgca gacccttgcc caaggccata tggtggggat cgcaattgcg gttgagcggg
      301 tcgtcgcctc cgccgatatt gtaacggaat atccggtaat tcagaccttc gggactgacc
      361 aaccagtcga tgatttcgtc tattttgttt tcctcccaac ggccgcacat attggcccac
      421 caacagagcg acacgcccca gccttcgaaa gtcttgccga aggcgtcgga gggattgatc
      481 aggtaataca ggctgtcggt gtcgacgggg aattccctga tttcttcgga gatcagccag
      541 taatgccttt cgtccaagcc actcttgtcg gaatagacat gtgagccggc tgtactgttg
      601 tctgtgccca ggtatttccg attggcctta caacgcaaac gtactatgcc ttttcgcact
      661 tcttcaacgg catatttggc ggcatcggaa gccgaatcgg atacaaaata agtgttccaa
      721 ctgcccgaga gcgccatgta ttcccggcga tccttgagct tgacggtata atagcccctg
      781 ccgtcggcta cgaagttcag cacctgggtt ccgatcgcac cggcagtctg caccacagcg
      841 cgtccgtcgt cggccctcga caaaacgcaa gcaccggaat ggatcaggta ataatcttta
      901 tcggattcag gttcaatcac ctgtgcactt ccgcctatag actgccctgc aaaaatcagc
      961 agcaagacta tttttaagtt caaagagaac gatcgcattt cattattcat tagtcgtaaa
     1021 actaaaatga ctttccggca atcacaacag aaaggacatt caggaggctg tgttggttta
     1081 ccaaacctga gctttttcag cctttattct cccaaataaa acgaaggcac cggtgcctga
     1141 tcgtaaccca tggagcgatg cgccacgtaa ttgcgataaa gagcatcctg catcagtgtt
     1201 atccggcgat cacgggccgg aatattggta gtgtacacgc gcaattcccc gggcaaggcg
     1261 gtaataattt cttctctcca atctccgaaa acatcggcta tcaccagaat actaccctcc
     1321 atttctccga ataatgtttc tcctttccat tttccaatac ccgatttcct gccgaaccgg
     1381 ccctcggggc tacggtaaaa ctgagcgctg atgtattcgc gcaacaaatc ggcgtcccac
     1441 caggcccagt tacggcaagg aggcacttct tcgtttttgc ctattatccg gccatcggca
     1501 ctcaataaat atttatcgct gctgccgcct ttttgatctt ccgaagcaaa acattccaaa
     1561 ccgggagagg acggatcgaa atcggctatc ataccgtcgc ccacgtgata agtcttgtgt
     1621 cctatattcc aaatgggaga gccgtctttg gggtcgatca tacaaactcc tcttccgtta
     1681 tcgtgccagg gctcaaggca gagcagcatt tccataccgg ggcgattggg atcgatgtcg
     1741 ctgataaaaa ttttgtcggg atgacccaga cccgtcgacc agagcagtgt accgttgtcg
     1801 tccagggcac aggagcccaa caccacttcg tcgcggccgt cgccgtcaac gtcggtgcaa
     1861 atcatgttgt gagcacccat gcttcggacc acagggtttt cttcgtcgcc gtcccatctc
     1921 caggccaatt ccagtttgtc gccccggagc atccaggcat ccacggtcat cagtttatag
     1981 gtgcctctgg cagccagaat ataaggtgtt ttgccgtcca aaaaagccat acctatctga
     2041 tgtctgctct gacgattgag atcgccgtaa cggaaattcc tcaccggcca gtcgacacgg
     2101 tctatttcgc gtcccgtcat accgtcgagc accgagaggt attcatcgcc atagtccacc
     2161 cggccttttt cgttgcgcac aatatcgccg ggagccgttt tcagggccac ctcggcctta
     2221 ccgtctccgt tgaagtcgta acaaataaaa ggcgaatacc atacgccggg ctctatgccc
     2281 tgacccaggt cgaaagacca gagaaaagtg ccatcgctca agtaagcttc gatttgatag
     2341 gttcttcccg tcaaatcgcc cggcataccg ggatcaacat tggaatcggg tgtacggata
     2401 atgtaatcgt aaacgccgtc gccattgagg tcggccagag ccatcttgcc cggcctgacg
     2461 ccgtctcgca gtttaatact tcgataggga aaggccggta aatcttccat ccggatgctg
     2521 atgctgcctt cgcctgtatt taggacacct ttagtcttat ccaggctggc cgcagccgag
     2581 cccggattag ccgcaggctt tgctccggca gaattcaccg actttcggtt gtttcgcggc
     2641 acaactttgt aaacagcctc agccggagga ttctcaacaa taaaatcaca ggtggctgta
     2701 atcggccgct tgttgagtcg ttgatttttt tgaccttcca cacgaatcac atcaaaggcc
     2761 acatcctgag ggtcctctgc caagagtctc caactcaggt agagcgactg cccgtcctga
     2821 gcccgtgttg ctgtcagtcc ccggtcaagg gcctcgttca ccctcatcgt agcaaaaccc
     2881 tccacctggg gtttaggggc gtgatggggt tctaagattt cataataata atacctttct
     2941 ctgggatcct gagccccggt cttgaacaga ccggcgaata agaataataa tgtcgctccc
     3001 ccacccaggg caaagcggac aagtgtagat tttgttttca tagtatcgac aaatgtcgta
     3061 ataaatttca aaatttattc ataaaaaaca tcaaacattt tgaggtgtaa aataatgcct
     3121 ccatctttgt aagagtgtgc gcttattttt tatcagccag tctttcaacc atgaacaaac
     3181 ttatattttc cctgggccta ctttttttta catgccctgt gtctgatgct cagggaacat
     3241 tccaacacag agccaccggc tccaccactc aaaccgaccg gcgatttgaa gggaccgccg
     3301 tccatacccg ggccggggca gaatccttgc ccgcgaccaa tcccattcat ccgcaacctg
     3361 ttgccgacag aatagttcct ttcaatctgt cggacagcgg caaacacacg ccgatactct
     3421 gggggctcga caccgcctgg cccagccgcg acaatatcgt acggggagtt gcttttatgg
     3481 gcagtgaaaa tatttatttg gtcagagctt cctttcaacc cacaatgccc ctggtgaacg
     3541 gagatctgca gtcgcaacaa gtagccgatc tcaataatcg cctcaatctt attaatctga
     3601 caggcagcca taccaaggta gccttgaatt gcgatcaccc cagcgtcgat ccctggtatg
     3661 ccggcaatgc ccaaaactgg gcacaactga tggatgtcac cgcccgtcgc atacaggaaa
     3721 gaggacgtcg gatagtatcg attgctccct ttaacgaacc ggattacggt tgggggcagg
     3781 gcagcttgac cgattttttc aacattgcag gcgaattgcg caacaatccc cgcttcgaca
     3841 gcattcgcat cagcggaggc aataccctga acaccgacca agccctgagt tggtataatt
     3901 ccctgaaaag ccgtctcgac gaaggaaaca cccaccaatt ggccggaagt ttcgataatt
     3961 acgcttcgtt tttcagaacg gtgcgcgcca acggacatca cgccaccaac gacgaattgc
     4021 ataatgtgat ggaagccatg gtcggcgtag aatacggtat gcagacgggc atttggtggg
     4081 gcaccgccga actggcccgg ggagaattcg tcaaagcgag ctttggccgc cgcctggcct
     4141 atgccgaaca caggcccaac tggacagcag caacggttta ccgctctccc gagggcaaag
     4201 tgcaggcctt tgtaggaagc tccgagcggc aggccgcaac cacttcttat agttttatct
     4261 ctttcgaacg cgacgtattt tacgacggac aaggccccca gcgagctttc acaatagaaa
     4321 tcccgggagg cacaggttat cagcaaggtc aaaccaatgc cgaaagagtg atcaacataa
     4381 actggggaga agatgtacct caacccgtca acggacgtta tgtactggta aaccggaaca
     4441 gcgccaaggt aatggaggtg gaaaacggct ccacaagcgc tggtagcaac gtgcgtcaaa
     4501 acacctacac cggggcagcc taccaacagt gggttgtaac cccggtagat ccccgcattg
     4561 ggggcgattt cagctatcac agcattattg ccaatcacag cggaaaatcg cttgat
//
LOCUS       DAOSGL010000177         4605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16750_length_4605_cov_4.395385, whole genome shotgun sequence.
ACCESSION   DAOSGL010000177 DAOSGL010000000
VERSION     DAOSGL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16750_length_4605_cov_4.395385"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1376
                     /locus_tag="PKY68_06215"
     CDS             135..1376
                     /locus_tag="PKY68_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24665.1"
                     /translation="MKNLNMLALIALILAFCSCSQEQEVNTAALDVQTIGGYVQKGPF
                     LNGTSITFSELSEEFVPTGKNFSTQINDNKGSFELLNLTLVSPYVELEANGFYYNEVK
                     NENSAAQLTLYALSDLTDKSSLNVNVLTHLERNRVKHLIANGLSFSEAKSQSQREILS
                     LFEIDKQNVANSELLDITKQGDDNAILLAVSVILQGHLSVSELSELLANISTDIREDG
                     LLNNPALGSMLINNAKYLNLENIRQHLENRYEALEMDVSIPDFEGYVNAFIENTDFVL
                     TRHIEYPAAGQYGLNILDREKTQYAAGDYSMKAVLPEGTNLKVKISGDNWVYPAMQNN
                     TGWDYSEWNATEKSRLFTSVKTGEIDFEIRFQYKENSGASQGDTINPPSGNTDLNKVN
                     LFVYENGTPEPTWTKEITITP"
     gene            complement(1452..1525)
                     /locus_tag="PKY68_06220"
     tRNA            complement(1452..1525)
                     /locus_tag="PKY68_06220"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1489..1491),aa:Met,seq:cat)
     gene            1696..2070
                     /locus_tag="PKY68_06225"
     CDS             1696..2070
                     /locus_tag="PKY68_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOH24666.1"
                     /translation="MKEAFRLEYSLKGSVPVLWKLISTPNGLSEWFADNVQAEDSIYT
                     FQWGRTSEQAEVVSMVQGESIRYRWLDEPEDTYFEFNIQYSELTGNVSLWVTDYAEPE
                     DQEDAVNLWNYEIEKLMRRSGM"
     gene            2149..3693
                     /locus_tag="PKY68_06230"
     CDS             2149..3693
                     /locus_tag="PKY68_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOH24667.1"
                     /translation="MFRIKRLHLFVLQSFFPIFLMTFLISNFILVMQLVWRVAEYIVG
                     KGIEFTAYLEFLLYAAATMVPLSLPLAVLLASLMTFGNFGEKLELLAMKSAGISLYNI
                     MRPLIVLMILLSVFSFVFQDRILPVAQMKLQSLTVSMQQKSPALSIPQGAFYSDIEGM
                     TIYVKEKDTKKKLLKDIMIYDFSQGFDNAAVTLADSGLLRLTEDKLNLVLTLYHGEGF
                     SNFDKQQAVTKDIPYRRESFREKEIIIPFDNNFNRIDESQFQSSHLSKKNDELKQVID
                     SMGSLLDSTKIKKKIQYTESHFMGRNTEKGRELIGRDSMARATEVPLFNPDIDSLFLT
                     LNKAQADNMLDKALKKVGTIRSEFEYQEIALSREHNQFRRSGIEYYRRYALSVACLVF
                     FFIGAPLGAIIRKGGIGMPAVVSVILFIAYYMIDAAGQKLVRNGTLEAFYGMFLSTAA
                     LLPLGIFLTYKSANDSAIFNLDSYIAFFKRIFNVKFTYRYLRYVFRVALKKKYYARKN
                     KTKQYR"
     gene            3659..>4605
                     /gene="ribB"
                     /locus_tag="PKY68_06235"
     CDS             3659..>4605
                     /gene="ribB"
                     /locus_tag="PKY68_06235"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOH24668.1"
                     /translation="MQEKIKLNSIDEAIEAIRQGEIIIVVDDEDRENEGDFITSAELI
                     TPEKVNFMITVGRGVLCAPITEERCLELELDKQVANNTSILGTPFTVTVDKLEGCTTG
                     VSSHDRAETIKALADPNTKPEDLGRPGHISPLYAKNKGVLRRAGHTEASIDLCRLAGL
                     RPAAALIEIMNEDGSMARLPELYKLAQKHKLKLIAIKDLIAYRLKQESLVEMGQSVQM
                     PTKYGFFKLIPFRQIATGQEHIALTKGEWSKDEAVLVRVHSSCLTGDVLGSMRCECGE
                     QLHKAMEIIEKEGKGVVVYMNQEGRGIGLMNKIQAYKLQ"
BASE COUNT         1332 a         1024 c         1036 g         1213 t
ORIGIN      
        1 gcctccgggt atgtacttgt ataaaattta tgccggtaaa tgctataccg gtaaagtgtt
       61 gttgccatga gaaatagcac gatgcttata tctcattgcc ttaattattt aattcaatat
      121 aaaatataaa cattatgaaa aacttaaaca tgcttgcgct tatagccctt atccttgcgt
      181 tttgctcctg ctcacaggaa caagaggtaa atacggcagc attggatgtg cagacgattg
      241 ggggctacgt tcaaaaagga ccgtttctga atggaacgtc cattactttt tcggaacttt
      301 ctgaagagtt tgtcccgacg ggaaagaatt tttcaaccca aatcaacgat aacaaaggaa
      361 gctttgagtt gttgaatctg acattggtct ctccttacgt cgaattagaa gcaaacggct
      421 tctactacaa cgaggttaaa aatgaaaatt ccgccgctca actaaccttg tatgctctat
      481 ccgatttgac ggataaaagc agtctgaatg tcaatgtgct cactcacctg gaaaggaata
      541 gagttaaaca ccttattgcc aacggcctga gtttttctga agcaaaaagt caatcgcaga
      601 gagaaatttt gtcccttttc gagattgata aacagaatgt tgccaattcg gaactgctcg
      661 acattacgaa acagggagac gacaacgcta tcttgttggc tgtttccgtg attcttcagg
      721 gacatctgtc tgtttccgaa ttgtccgaac tattggcgaa tatcagcacg gacatacggg
      781 aagacggtct tttgaacaac ccggctttgg gctcaatgct gatcaataat gccaaatacc
      841 tcaacttgga aaatattcgt caacatctgg aaaaccggta cgaagcttta gagatggacg
      901 tatccatacc cgattttgaa gggtatgtaa atgcttttat cgaaaatacc gactttgtgc
      961 tcacccgtca tattgagtat ccggcagcag gccagtatgg tttgaacata ctcgatagag
     1021 aaaaaaccca atacgcggcc ggagactatt ccatgaaagc cgtattgccc gaaggaacca
     1081 atctcaaggt gaagatcagt ggcgataact gggtgtaccc tgcaatgcag aataataccg
     1141 gctgggatta ttcggaatgg aacgcaacgg aaaaatcacg cctgtttact tccgtaaaaa
     1201 ccggagagat tgatttcgag atacgatttc aatacaaaga aaattccgga gcttctcagg
     1261 gtgatacgat caatcctcct tcgggaaata ccgatttgaa taaggtaaac ttattcgttt
     1321 acgagaacgg caccccggaa cctacctgga caaaagaaat aacgataacg ccctaaatcg
     1381 ggcaggtcga taatttggat tgttcggtaa tccaatacaa aaagagggca aattgccctc
     1441 ttttttgatt gtagcgagac ccgggattga accggggacc tcatgattat gaatcatgcg
     1501 ctctaaccgg ctgagctatc tcgccatctg cttgcccaaa agcgcggcaa aggtaatctt
     1561 tttagtttat ccaaccaaac agggaaataa aaaaatcccc aactttttga aaactatccg
     1621 gaatttatcc ttctattgtg gctcattatt cagaaaaaat atgtactttc ggcaaaattc
     1681 aaaaaaacgc tcgtgatgaa agaagctttc aggttggaat actctcttaa gggatctgtc
     1741 ccggtgctgt ggaaactgat aagcacaccc aacggtctgt cggaatggtt tgcagacaat
     1801 gtacaagcag aagattcgat ttatactttt caatggggta gaactagcga gcaggccgaa
     1861 gtggtttcta tggtacaggg agaatccatt cgctaccgtt ggcttgacga accggaagac
     1921 acttatttcg aattcaacat acaatattcg gaactgaccg gcaacgtctc tttgtgggtt
     1981 accgattatg ctgaacccga agatcaggag gacgccgtca atctttggaa ttacgagatt
     2041 gaaaaattga tgaggcgctc cggcatgtaa tctcgtgcat aattcctgct ttgctgtgcc
     2101 cggctcgttt ttttagatta ccttcgcagg cttaaacaaa gtcgatttat gtttagaata
     2161 aaaaggttac atctctttgt tctgcaaagt tttttcccca tctttttaat gactttcctc
     2221 atcagcaatt ttattctggt gatgcaattg gtgtggagag tggcagaata cattgtcggc
     2281 aaaggcatag aatttaccgc ctatctggaa tttttgctct atgctgctgc caccatggtt
     2341 cccctgtcgc ttcctttggc tgttttgctg gcctccctga tgaccttcgg caatttcggt
     2401 gaaaagctgg agctcctggc catgaaatcg gccggtattt cgctttacaa cattatgcga
     2461 cccctgatag tgctgatgat cctgttgtcg gtcttttctt ttgtttttca ggacaggatt
     2521 ttaccggtgg ctcaaatgaa actgcagtcg ctgacggttt ccatgcaaca aaaatcgccg
     2581 gccttatcca ttcctcaggg agctttttac tccgatatcg agggcatgac catttacgtc
     2641 aaagaaaaag acaccaaaaa gaaattgctc aaagacatca tgatttatga tttcagccag
     2701 ggtttcgaca atgcggcagt aactctggcc gattccggat tgttgcgact taccgaggat
     2761 aaattaaacc tggttttgac cttataccat ggcgagggct tcagcaattt tgataaacaa
     2821 caggccgtca ctaaggatat accctatcgt cgcgaaagct tcagggaaaa agaaatcatc
     2881 attcccttcg ataataactt caaccgtatc gatgagtctc agtttcaatc ttcgcatctg
     2941 agcaaaaaaa acgacgagct caagcaggta atcgattcta tgggcagcct gctggacagc
     3001 acaaaaatca aaaagaaaat acagtacacc gaaagtcatt ttatgggtag gaataccgaa
     3061 aaaggtcgtg aactcatcgg cagagattcc atggctcgcg ctacggaagt tccgcttttc
     3121 aatcccgaca tagacagttt gttcctgact cttaacaaag ctcaggcaga caatatgttg
     3181 gacaaggccc tgaaaaaagt cggcacaatc agatcggagt ttgaatacca ggaaattgcc
     3241 ttatcgaggg aacacaacca gtttagacgc agcggtatcg aatattaccg ccgctatgcc
     3301 ctctcggttg cctgcctggt tttctttttt atcggcgcac ccctgggagc cattattcgc
     3361 aagggcggca taggcatgcc tgccgtggtt tcggtgattt tgtttatcgc ttattacatg
     3421 atagatgcag ccggccaaaa attggtccgc aacggtaccc tggaagcttt ttacggtatg
     3481 ttcctgagca ccgctgcctt gttgcctctg ggcattttcc ttacctataa atcggccaac
     3541 gactccgcca ttttcaattt ggattcctat atcgcttttt tcaaaaggat attcaatgta
     3601 aaattcactt accgctattt gcgctatgtt tttagagttg ctttaaaaaa gaagtattat
     3661 gcaagaaaaa ataaaactaa acagtataga tgaggctata gaagccattc gacagggaga
     3721 aatcatcatc gtggtcgacg acgaagaccg cgaaaacgag ggcgacttta ttacctcggc
     3781 cgaattgatt acgcccgaga aagtaaactt tatgattacc gtggggcgcg gcgtactctg
     3841 tgcacccatc accgaagaac gttgtttgga attggagttg gataaacagg tggcgaataa
     3901 cacctccatt ttgggaaccc cctttacggt gaccgttgat aaactggaag gttgtaccac
     3961 cggggtatct tcgcacgatc gcgccgaaac catcaaggct ttggccgatc ccaacaccaa
     4021 acccgaagac ctgggacgcc cgggacacat cagtcccctt tacgccaaaa acaagggagt
     4081 attgcgccgg gcgggacaca ccgaagcttc catagacctg tgccgtctgg ccggtttaag
     4141 gccggcagcc gccctgatag aaataatgaa cgaagacggc agcatggccc gtctgcccga
     4201 attgtacaaa ctggcacaaa aacacaagct gaaactcata gccatcaaag acctgattgc
     4261 ttaccggctc aaacaggaat ctttggttga gatgggtcaa agcgttcaga tgccgaccaa
     4321 atatggattt ttcaaattga ttcccttcag gcaaattgct accggtcagg aacatattgc
     4381 tctgaccaag ggagaatgga gcaaagacga agctgtattg gtcagggtac actcttcctg
     4441 ccttacggga gacgtactgg gttctatgcg ctgcgaatgc ggcgaacaat tgcacaaagc
     4501 gatggagatc atcgaaaaag aaggcaaagg cgttgtagtt tacatgaatc aggaaggccg
     4561 cggcatcgga ctgatgaaca aaattcaagc ctataaattg caaga
//
LOCUS       DAOSGL010000178         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16767_length_4602_cov_4.285463, whole genome shotgun sequence.
ACCESSION   DAOSGL010000178 DAOSGL010000000
VERSION     DAOSGL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16767_length_4602_cov_4.285463"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2491
                     /locus_tag="PKY68_06240"
     CDS             <1..2491
                     /locus_tag="PKY68_06240"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOH24669.1"
                     /translation="SITKIRLNSRPLIENFEGKSGVVWRLSQRTTENNLAAANCVPLG
                     DIINISDKMDESGNLTNLDLPQGDWLILRMGHTSTGHENETGGGGRGLECDKFNPEAV
                     RLQFDNWFGAVYKHVDKSLLKGVLTNMHSDSWECGSQNWTPNFLEEFRQRRSYDLLPY
                     LPLYAGIPIGSADISESVLLDIRQTVTELVNDVFFATMSELAAQKNCVYSSECVSPTM
                     LSDGMLHYQRVDLPMGEYWLDSPTHDKPNDMADAISGAHIYGKNLVQAEGFTQLRGEW
                     NEHPRMVKTLGDFNFAQGVNKLFFHVYCHHPFIGKYPGMTLDGIGLFMQRGQTWWPYA
                     SEWVNYLSRCQAMLQRGVPVNDIAVFTGEELPRRAILPDRLVASLPGLFGEEALASEQ
                     LRKLNQGAPVHSTSVNVTASANMYTANQWIDPLRGYKYDSFNKDAFLRLARAENGRMV
                     LPGGAGYRVLVFPEPHQMAPDAGYMSLEVARKIKELQDAGVIVLLPSERPVCIPDYHQ
                     KEQRNRELEQLTHAIWEKAQQQACLLPFKEENMNRFGLEADVLFKDLQGKELYDLAWN
                     HRREGASDIWFISNQLDSIQELEISLRGAGRIPEWFDPQRAEIAEIKNWRIEQGRTIV
                     RLDLSPAQSGFIVLRNKTTQESNNRPEQYTTSGLDLDPWKIRFDALDKSMESTDLFDW
                     RKSDNMDIKYYAGTAEYHSAFTLDKIPEAEKIYLKFDSVNMMAEVFLNGKSCGIVWTQ
                     PYLLEISSALKTGKNELKINVANTWYNRVQAINHKLIQEDDYWTNARIWRVNRPRSRE
                     GFSLQNFPVEYLQESGIQGDIKLVWTINQAQ"
     gene            2493..3956
                     /locus_tag="PKY68_06245"
     CDS             2493..3956
                     /locus_tag="PKY68_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOH24670.1"
                     /translation="MKYFVHLLLLLFVLVLPEKMEAKLRLPAFISNNMVLQQQQETKI
                     WGWSESGNSVRLKASWDNKQYATKANSEGYWELKFNTPEAGGPYTILLDDGEELLLEN
                     VLIGELWFCSGQSNMEMPMKGYPAQGIEGGLQDLMHSKDDQLRLFTVKRAALASRAND
                     VQGHWQEASPGTVADFSATAYYFGRYLRELLGVPVGLINSSLGGSFIEAWMSAESLEG
                     LSDLPIPRTQEEVRSSTHTATALYNGMVHPFLGMNIKGCIWYQGESNTGRPESYKELL
                     TQMVSSWRKDWGIGDFPFYYCQIAPYDYALITPQGEQAVNAALLREAQAQVEDLIPNC
                     GMAVLMDAGLISDIHPRKKRQAGERLALLALVDSYGLESPEHRSPRYSACEQRDSMLI
                     LSFDRAGLGLDRTRGSLKHFWIAGQDKVFYPAQAKINRDKIQLWSASVPKPVAARYAF
                     DNISEGDLFGVNGQAVSSFRTDNWSRDEIKFFEKHEK"
     gene            3946..>4602
                     /locus_tag="PKY68_06250"
     CDS             3946..>4602
                     /locus_tag="PKY68_06250"
                     /inference="COORDINATES: protein motif:HMM:NF039200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HOH24671.1"
                     /translation="MKNSKHLFLSLVLLSAWTFGFAKPADLLKDYELTWTTQSKGSNE
                     SMPCGGGDIGMNVWVEQGELLVYFSRSGSFDENSCFLKGGRLRLSFPDEPLDTVEFLQ
                     CLKLSRGLVNIQAGSKGNKVDIDVWADVFKPVIHLDIRSASPRKAMISYENWRYRDRL
                     LRKNESQSNSYKWAPPADLKTSADKIDINENGIVFYHQNPSSGTMFDVVVQQQGMEEV
                     K"
BASE COUNT         1232 a         1037 c         1192 g         1141 t
ORIGIN      
        1 cagtattacc aaaatcaggc ttaacagccg gcccttgata gagaattttg aaggaaagag
       61 cggagttgtc tggcgtttaa gccaacgaac cacagaaaac aacttagccg ccgccaattg
      121 cgttccactt ggagatatta tcaacatcag cgataaaatg gatgagtccg gcaacttgac
      181 caatctggac ctgccgcagg gcgactggct gatattgagg atgggacata cttccacggg
      241 ccatgagaat gaaaccggag gcgggggcag gggcctggaa tgcgataagt tcaatcccga
      301 agccgtccgt ctgcaattcg acaattggtt tggtgccgtt tataaacatg tggacaagag
      361 cttactaaaa ggcgtattaa caaatatgca ttccgatagt tgggaatgcg gtagccaaaa
      421 ctggacccca aattttctgg aggaattccg tcaacgccgt tcctacgatc ttttacccta
      481 tttgcccctc tacgccggca ttcccatagg cagtgcggat atttcggaga gtgtgttgct
      541 cgatattcgc caaaccgtaa cagagttggt gaatgatgtg ttttttgcca ccatgagcga
      601 gctggccgca cagaagaact gcgtttattc ttcggaatgc gtttccccga ccatgcttag
      661 cgacggtatg ctgcattatc agagggtgga tctgcccatg ggcgagtatt ggctcgacag
      721 tcccacgcac gataagccca acgatatggc cgatgccatt tcgggagcac atatttacgg
      781 aaaaaatctc gtgcaggccg aaggctttac ccagttgaga ggcgaatgga acgaacatcc
      841 ccgtatggtt aaaaccttgg gcgatttcaa ttttgcccag ggggtgaaca aattattctt
      901 tcacgtgtat tgtcatcatc cctttattgg aaaatacccc gggatgaccc tggacggcat
      961 aggtttgttt atgcaaagag ggcaaacatg gtggccctat gcttcggagt gggtcaatta
     1021 ccttagccgt tgccaggcca tgctacagcg gggtgttccg gtaaacgaca ttgccgtgtt
     1081 tacgggagag gagcttcccc gcagggccat tttacccgat aggctggtgg cttcgttgcc
     1141 cggcttgttt ggagaagaag cccttgcctc tgaacagctt cgcaaattga accagggcgc
     1201 acccgtgcat tctacttcgg tcaatgtaac ggcttcggcc aatatgtata cggccaacca
     1261 atggatagat cctctgagag gttataaata cgattctttc aacaaagacg cgttcttgcg
     1321 attggcccgg gccgaaaacg gcaggatggt tttacccggc ggagccggtt acagagtgtt
     1381 ggtatttccc gaaccgcatc agatggcgcc cgatgccggg tatatgagtc tcgaggtagc
     1441 ccgcaaaata aaagaactac aagatgccgg agtcattgtg ctcctgcctt ctgaacgtcc
     1501 ggtctgcatt cccgattatc atcaaaaaga gcaacgcaac cgggaattgg agcaactgac
     1561 tcatgccatt tgggaaaaag ctcaacaaca agcatgtctt ttacctttca aagaagaaaa
     1621 catgaatcgc ttcggtttgg aagccgatgt cctgttcaaa gatttacagg gcaaagaatt
     1681 gtatgatctt gcctggaatc atcgcagaga gggagcaagc gacatctggt ttatatccaa
     1741 ccaacttgat tcgattcaag aacttgaaat ttccctgcgc ggagcaggcc ggataccgga
     1801 gtggtttgat cctcaaagag ccgaaattgc cgaaataaaa aattggcgca tagaacaagg
     1861 caggaccata gttcgcctgg atttgtctcc tgcacagtcg ggttttattg tcctaaggaa
     1921 taaaactacg caagaaagca ataatcggcc ggagcagtac acaacttccg gcttagactt
     1981 ggatccttgg aagatccgtt tcgatgcact cgataagagc atggagagta cggatttatt
     2041 cgattggcga aaatcggaca atatggatat taaatattac gcaggaacag cagaatatca
     2101 ttcggctttc acacttgaca aaattcccga agctgagaaa atatatctga aattcgacag
     2161 tgtgaatatg atggccgaag tttttctgaa cggaaaatcc tgtggcattg tttggaccca
     2221 accctactta ttggaaataa gttcagcatt gaaaaccgga aaaaacgagt tgaaaatcaa
     2281 tgttgccaat acctggtaca atagggtgca ggccatcaat cacaagctga ttcaggagga
     2341 cgattactgg acgaatgctc gtatatggag agttaacaga cctcgttcgc gagaaggttt
     2401 cagtttacaa aacttccctg ttgaatattt gcaagagtca ggtattcaag gggatattaa
     2461 attagtatgg acgataaatc aagctcaata gtatgaaata tttcgttcat ttgcttctct
     2521 tgctctttgt gttggtgttg cccgaaaaaa tggaggcaaa attgcgttta cctgccttta
     2581 tttcgaacaa catggtgttg caacaacaac aggaaaccaa aatctgggga tggtcggaat
     2641 ccggtaacag cgtcaggctt aaagcctcct gggacaacaa acaatatgca accaaggcaa
     2701 acagcgaagg ttattgggaa ttgaaattta ataccccgga ggccggcggg ccttacacca
     2761 ttcttttgga tgacggagag gaacttttgc tcgagaatgt tttgatagga gaactttggt
     2821 tttgctccgg tcaatccaat atggagatgc ccatgaaggg ctatcccgca cagggaatag
     2881 agggcggcct gcaggacctg atgcattcaa aagacgatca gttgcgtttg tttactgtca
     2941 aaagagcagc cctggcttcc cgggccaatg atgtacaagg ccattggcag gaagcttcgc
     3001 ccggaacggt agccgacttt agtgccactg cttattattt cggccgttac ctgcgggaat
     3061 tgctcggagt gccggtaggc ttgataaaca gcagtctggg cggatctttt atcgaggcat
     3121 ggatgagcgc cgaaagcctc gaaggtctgt cggatttgcc tatcccacga actcaggagg
     3181 aagtgcgctc ttccactcat acggcaacag ccctgtacaa cggcatggtt catcccttcc
     3241 tcggtatgaa catcaaaggc tgtatttggt accagggaga atccaatacc ggccggcccg
     3301 aaagctacaa agagctctta acgcaaatgg tttcaagttg gcgtaaagat tgggggatag
     3361 gggatttccc tttctattat tgtcagattg ctccctacga ttatgccctg attacacctc
     3421 agggcgaaca agcggtgaat gccgccctgc tcagggaagc tcaggcacag gtcgaagact
     3481 tgatccccaa ttgcggtatg gccgtgttga tggatgccgg tctgattagc gatattcatc
     3541 cgagaaaaaa acgacaggcc ggggaacgtc ttgccctatt ggctctggtg gacagctacg
     3601 gactggaaag cccggaacac cgcagccctc gctactcggc ttgtgagcag cgcgacagta
     3661 tgcttatact gagttttgac agggcagggc tgggtctcga cagaacaagg ggcagcctga
     3721 agcatttttg gatagccggc caagacaagg ttttttatcc agctcaggcc aaaatcaacc
     3781 gggataagat tcagttatgg tctgcctcgg ttcccaaacc cgttgcggcc cgttacgcct
     3841 ttgataatat ttcggagggc gatttatttg gcgtgaacgg ccaggccgtt tcctctttca
     3901 gaaccgataa ttggtcccgg gatgaaataa aattttttga aaagcatgaa aaatagtaag
     3961 cacctttttt taagcctggt tttgttgtcg gcctggactt ttggctttgc caaacctgct
     4021 gatttactga aagattacga actgacctgg actactcaga gtaaaggttc gaacgaatcc
     4081 atgccctgtg ggggcggaga cataggtatg aatgtctggg ttgagcaggg ggaattattg
     4141 gtgtattttt cgcgtagcgg cagtttcgac gaaaacagtt gcttcctcaa aggcggtcgc
     4201 cttcggttga gttttcccga tgagcctttg gatacggttg agtttctgca atgtcttaaa
     4261 ctgagccggg gtttggtcaa cattcaggcc ggcagcaaag gcaataaagt ggacatcgat
     4321 gtatgggccg atgtgtttaa gccggtcatt catttggata ttcgctctgc cagtccccgg
     4381 aaagcaatga tctcctacga aaattggcga taccgcgaca ggttgctccg taaaaacgaa
     4441 agtcagagca actcctacaa atgggctccg ccggctgatt tgaagaccag tgccgataaa
     4501 attgatataa acgaaaacgg catcgttttt tatcatcaaa atccctcctc cggcaccatg
     4561 ttcgacgtgg tagttcagca acagggcatg gaagaggtga ag
//
LOCUS       DAOSGL010000179         4591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16838_length_4591_cov_3.434303, whole genome shotgun sequence.
ACCESSION   DAOSGL010000179 DAOSGL010000000
VERSION     DAOSGL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16838_length_4591_cov_3.434303"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PKY68_06255"
     CDS             complement(<1..1635)
                     /locus_tag="PKY68_06255"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOH24672.1"
                     /translation="MLRKTILGFVLLLVGLPGYGQEPYSIDVSGVKTGVIRHHLDLGG
                     SNAEGDSIAVNSFYIERRGEPYIPVIGEFHYARFPRQYWDEEIKKIKAGGVSVVATYV
                     FWILHEFKEGEFDWSGDLDLRYFAELCGQNGLDMIVRIGPFAHGEMRNGGLPDWLYGR
                     PIEVRSNDPAYLFYVDRLYRQIGQQLQGLLFKEGGPVIGIQIENEYQHSAAPWAFTYE
                     GAPRENTVARRDVDITKIGVGINEFGNEFAGLGRDHMKTLKQLALDAGLETPLYTATG
                     WGYATIIEKGSIPVMAGYAYPFWVDSNQPSPFFLYKDIRQNPDYAPVSYDVDLYPSLA
                     AELGTGISVTYSRRPRVPGESFLPMMVRTIGSGSNGLGFYMYHGGNTPSSGNFFTAEG
                     FGLANKSYDYQSPLGEFGLPSKGYYPLRLINYFLQNYGGLLAPMYPVLPATNAQIKAE
                     TTDILRYALRSDGEGGFLFMHNFQDRISLPDQEQISIEIKTKNGQLRFPVQGSFTLKS
                     GASAIFPFNLNLDGVELVQATLQPFMRFEQGGRKYHA"
     gene            complement(1681..2151)
                     /locus_tag="PKY68_06260"
     CDS             complement(1681..2151)
                     /locus_tag="PKY68_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24673.1"
                     /translation="MKKNLCLIAAFLFVGISYGQEDSVPERSRNVLAFGYQIGGYTLI
                     GVNYEIRVHDYFGIHFGMGYSGYTAGLKIHTNEKKNSPFFNISYKDGGFGLLKAFGTE
                     FGGRLVFNKKSEFGLHFQIGLAKILEIEENFEELLFNNKKAPPYMLSLGIGFSW"
     gene            complement(2148..4478)
                     /locus_tag="PKY68_06265"
     CDS             complement(2148..4478)
                     /locus_tag="PKY68_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH24674.1"
                     /translation="MKRFKLSLIVLLLLCVLPVRADLVVQAEDGVYVGKVDTQHAGYT
                     GRGFVDFTNATGSSLLLEFSLAEAMPEATVLVRWANGKSDDRSMSFRVNDELQIESQA
                     FGSSGSFTTWVETAVTLNLRKGTNRLLMTALTSNGGPNMDKITIVGGEEGLREYALNL
                     EIQGKGRVLKTPDAPFYPQGSTVELHAFPDTAFDASFVGWSGDLSGSDSIKLLYMDSE
                     KTVTATFKSNRHSVFYCAPAEKGGNDDNDGSIESPFFNISKALSLMEPGDTLFLRGGI
                     YRYTATIVLDQKGSEQERICIFNYPGENPVLNFYDIFSSYTNLNSSARGSARGFKITG
                     DYYYLKGLEICQAPDNGIKIEGSHNICELLVLHHNGDSGIQIGLAKNDPDAPDRVANN
                     LIKNCDSYRNLDWGTGYENADGFACKLSPGANNRFTGCRAWQNADDGWDFYMTRYPIY
                     VDSCWTFGNGNSELASKDDLDWEFGQKNTIPTSWSGDGNGFKLGGDGWAAKHEIRHCI
                     AFDGYQTGVGFNENNNADSLFLFNCVSWQGVKNYRLRAYPCDVRNCISFDAKTSGQTQ
                     LYNLAEGTVSKNNSWDQIDGEVLVPYKTATNEIFDQKSIYDEFVSTSKEDFLAPRQAD
                     GSLPDNGFGRLKANSIFIDRGSNIVRGVDPVSFRPIDIELNNYFGAAVDLGAYEYVQP
                     DGLPKIKSLSPRLKVSPNPCLQDCIVEMEFTEKGSAELQVFDLAGKMLHVQELSEVMA
                     GEKRRLQLNLPLPVGVYQLVLLNEKTQETLKLIKLR"
BASE COUNT         1156 a         1185 c         1119 g         1131 t
ORIGIN      
        1 tgcatggtac ttgcgcccgc cctgctcaaa gcgcataaaa ggctgtaacg tagcttgtac
       61 cagctccact ccgtctaaat ttagattgaa aggaaaaatg gccgacgccc ccgatttcaa
      121 ggtaaaactg ccctgaacgg gaaagcgcag ttgtccgttt ttggtcttta tctctatact
      181 gatttgttcc tgatcgggca agctgatcct gtcctgaaaa ttatgcataa acaaaaagcc
      241 gccctcaccg tcggaacgca gggcatacct caggatatcg gtagtttcgg ctttgatctg
      301 cgcattggta gcaggcaaaa cgggatacat aggcgccagc agtccgccgt agttttgcag
      361 aaaataattg atcaatctca ggggataata acccttgctc ggcaggccaa actcacccag
      421 gggcgactga tagtcgtacg atttattggc cagaccaaaa ccttcggccg taaagaaatt
      481 gcccgatgaa ggcgtattgc ccccgtgata catgtaaaag cccaaaccgt tgctaccgct
      541 gccgatggtc cgaaccatca tgggcagaaa actttcgccc ggcacccggg gacgtcgcga
      601 ataggtgacg gaaatgcccg ttcccaactc ggccgccagc gaaggataca gatccacatc
      661 gtagctaacc ggtgcatagt cagggttttg acgtatgtct ttgtaaagaa aaaaaggcga
      721 aggctggttg ctgtccaccc aaaaaggata agcatagccg gccatcaccg gaatggagcc
      781 tttttcgata atggtggcat aaccccagcc ggtagccgtg tacaggggcg tttccaggcc
      841 ggcgtccagc gccagttgtt tgagggtttt catgtggtct cgccctaaac cggcaaattc
      901 gttgccgaat tcattgattc ccacccctat tttggtaatg tccacatcgc gccttgccac
      961 cgtattctcg cggggagcgc cctcgtaagt aaaagcccag ggggccgccg aatgctggta
     1021 ttcgttttcg atctgtatgc cgataaccgg gccgccctct ttgaacagca gaccctgaag
     1081 ctgttgacct atctggcgat agagccggtc gacgtaaaaa aggtaagcgg gatcgttgct
     1141 gcgcacctct atgggacggc cgtaaagcca atcgggtaaa ccgccgttgc gcatttcgcc
     1201 gtgagcaaaa gggccgatac gcacaatcat atccagcccg ttctgcccgc ataattccgc
     1261 aaaataacga agatccaggt cgccgctcca atcgaactcg ccctctttaa actcgtgcaa
     1321 aatccaaaag acataggtgg ccaccacgct gacccctccg gccttgattt ttttgatctc
     1381 ctcgtcccag tactgacggg gaaaacgggc ataatgaaac tccccgatca ccggaatata
     1441 aggctctccc cggcgctcaa tataaaaact gttgacagca atggagtcgc cctctgcatt
     1501 ggaaccgccc aaatcgaggt gatgacggat taccccggtt tttactcccg aaacatcgat
     1561 ggaatacggc tcttgtccgt agcccggcag gcctactaaa agtaaaacaa agcccaaaat
     1621 agtttttcgt aacataacaa attagattta agtaaggcaa tgcgtggcag cgatcaaaaa
     1681 tcaccagcta aacccaatgc ccaacgacaa catatagggt ggcgcttttt tgttgttgaa
     1741 taataactct tcgaagtttt cttctatttc cagtatttta gccaatccta tttgaaaatg
     1801 aagaccaaat tcacttttct tattaaaaac caatcttccg ccgaattccg taccgaaagc
     1861 tttaagtaaa ccgaaaccgc cgtctttata gctaatattg aaaaaaggac tgttcttctt
     1921 ctcattagta tgtattttta gcccggctgt atatccggaa taccccatcc caaaatgaat
     1981 tccaaaataa tcgtgaaccc tgatctcata attaaccccg ataagagtgt atcctcctat
     2041 ctgatatcca aaggccaata cgtttctact ccgctccgga acagaatcct cttgtccgta
     2101 agaaatccct acaaagagaa aagccgctat taaacacaaa ttttttttca tctcaatttt
     2161 atcagtttta aggtttcctg cgttttctca ttgagtaaaa ccaactgata gacgcccacg
     2221 ggcagaggga gattcagttg gagacggcgt ttttcgccgg ccattacttc gcttaattct
     2281 tggacgtgga gcatcttgcc ggccaggtcg aatacttgca gctcggcact gcccttttcg
     2341 gtaaattcca tttcgacgat gcaatcttgc aggcagggat tgggcgaaac tttcaaccgg
     2401 gggctgaggg acttgatttt tgggaggccg tcgggctgca cgtattcgta agctcccaga
     2461 tcgacggctg cgccaaaata attgttcaac tcaatatcta tggggcggaa actgacagga
     2521 tcaacgcccc gcacaatgtt gctgccccgg tcgataaaga tagaattggc tttgaggcgg
     2581 ccgaagccgt tgtcgggcag gctgccgtcg gcctgacggg gagccagaaa atcttccttg
     2641 gaagtgctga caaattcgtc gtatatggat ttctgatcaa aaatctcgtt tgttgcggtc
     2701 ttgtacggca ccaacacctc gccgtcgatc tgatcccagg agttgttttt actcaccgtg
     2761 ccttcggcta aattataaag ttgagtctga ccgcttgttt tggcatcgaa gctgatgcag
     2821 ttgcggacat cgcatggata ggcccttagg cggtagtttt tgacgccctg ccaggagaca
     2881 caattgaaaa ggaacaagct gtcggcgttg ttgttctcgt taaaaccgac tccggtctgg
     2941 tagccgtcga aggcgatgca atggcggatc tcgtgtttgg ctgcccagcc gtcgccgccc
     3001 agcttaaagc cgtttccatc gcccgaccac gaagtgggga tagtgttctt ttgcccgaac
     3061 tcccaatcca ggtcgtcttt gctggccaat tcggaattgc cgttgccgaa agtccagcaa
     3121 ctatccacat agatggggta tcgggtcatg taaaagtccc agccgtcgtc ggcgttttgc
     3181 caggccctgc aacccgtaaa acggttgttg gctccgggcg aaagcttgca ggcaaagccg
     3241 tcggcgtttt cgtaaccggt cccccagtcg aggttgcggt aggaatcgca atttttgatc
     3301 aggttgttgg ccacccggtc gggagcatcg gggtcgttct tggccaggcc gatttgtatg
     3361 cctgagtcgc cgttgtggtg gagaacgagt aactcgcaaa tattgtggct gccttcgatt
     3421 ttaatgccgt tatcgggggc ctgacagatt tcgaggcctt tgaggtaata ataatctccg
     3481 gtaattttaa agccccgggc cgacccccgg gccgacgaat tcaaattggt ataagaggaa
     3541 aaaatgtcgt agaaattcag gacggggttt tcgccggggt aattgaaaat acaaatgcgt
     3601 tcctgctccg aacccttttg gtcgagcacg atggtggcag tgtaacggta aatgccgccc
     3661 cgtaaaaaga gcgtgtcgcc gggctccatc agactgagag ctttgctgat attgaaaaag
     3721 ggcgattcaa tgctgccgtc gttgtcgtcg ttgcctcctt tttcggcggg cgcacaataa
     3781 aaaacgctgt gacggttcga tttaaaagta gctgtgacgg ttttttcgga gtccatgtaa
     3841 agcaatttga tcgaatcgct tccgctcagg tcgccgctcc agcccacaaa gctcgcatcg
     3901 aaggccgtat cgggaaaagc atgcagttcc acggtactac cctgcgggta aaaaggagca
     3961 tcgggcgttt tcagcacccg gcctttacct tgaatctcga ggttcagtgc gtattccctg
     4021 aggccttctt cgccgccgac tatagtgatt ttgtccatgt tcggtccgcc gttcgaagta
     4081 agagccgtca tcaaaagccg gttggtacct ttgcgtaaat tcagcgttac cgccgtttct
     4141 acccaggtcg taaaagagcc cgaactgcca aaagcctgac tttctatttg caattcatcg
     4201 tttaccctga aactcatgga acggtcgtcg ctcttgccgt ttgcccacct caccagcacc
     4261 gtcgcctcgg gcatggcctc ggccagactg aattcgagca gcaaactgct gccggtggca
     4321 ttggtaaaat ccacaaaacc cctaccggta taaccggcat gttgggtatc gactttgcct
     4381 acataaacgc cgtcctctgc ctgaacaacc aggtcggccc ggacaggcag cacgcataac
     4441 aaaagcaaaa caatcaggga gagcttaaat cttttcacgg cagacagaaa tcaattaaaa
     4501 aaaatgcaag caaaaataag catttatttt gttttcaagt tcattaaagc tttttgcaaa
     4561 tgattaaaaa acgatacggg caggtataag g
//
LOCUS       DAOSGL010000180         4587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16862_length_4587_cov_3.550750, whole genome shotgun sequence.
ACCESSION   DAOSGL010000180 DAOSGL010000000
VERSION     DAOSGL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16862_length_4587_cov_3.550750"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2748
                     /locus_tag="PKY68_06270"
     CDS             <1..2748
                     /locus_tag="PKY68_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOH24675.1"
                     /translation="AKQAYEGRLKTLVLLVAFQDKAFVSPTAHDDFDRLSNELGYSDN
                     GATGSVRDYYRDNSNGVFDPEFVVMGPYTLSKPMTYYGGNNEEGNDNDPRRMVIEAAW
                     MANEEGVDFSEFDQNNDGYVDFIYVYYAGYNEAEGGPAASIWPHSWSVAYMDSTLLDG
                     VKLGYYACSSELAGASGSTMSAIGTVCHEYGHTLGLMDVYDTDGAESGGMSGGLGALS
                     LMSSGNYNNKSRTPPHLTSEERWSLGWMTPTTITEAGDYSLASIDQNQAYIIPTLNEN
                     EYFLLENRQHNNWDLYIKGEGLLIYHVDKSKNMVLGKTALERWYTNTVNVYPQHQCMD
                     MEEAVGSESKGDMYAFYPGPSGLYTAFTRTTTPAALAWSGLPLGKELYDIRLEGQEIH
                     FRVELEDQVYFGGQVINREGQPVQGALLYLKELVEQPVSGNTTGLRSAMHISGVYYTT
                     TSGIDGRFIFDEDLDPGVYELLCQSEAYISNSQIVELKPGSNSVLITLLTEAEQLIEG
                     TLSWCRDFSYGGAIGNEGESFVAGASWTAEELSQYRGMYIGRMTFFIAGGNPDVEVSI
                     YADRNPIFKKNIAAEDLTANSFYSLNLMADSLVINPETELILAYQVSNYPKNAYPAGI
                     CGGSRVEGKGNLISLDQGATWTTLYQEAEIEGNWLISFDIYRGSDFVAMESFNLDPTD
                     LTMKIGETRYLYGQIKPANATNGKISWSSSDSSVVWVDQTGKVFALAAGQSVVTATTD
                     GGNRSATCQVKVVPTIASSLNVNVAQHVFFVDWDEATGDAWTFSWRNLGDSVYQDTTL
                     TQSSFLLEGLDPKSTTEVLVQAYSEQQLVDEHAFSVTTANKKSNFMAIYTTETFSPGQ
                     KLLLDVLNLPEDASVNVWKLNDEVVSGSILELSEGIHELRVEITTSKGIEIIRRQLKV
                     E"
     gene            2750..3175
                     /locus_tag="PKY68_06275"
     CDS             2750..3175
                     /locus_tag="PKY68_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24676.1"
                     /translation="MRRYFSILALILLTGLLYLACVREDPLLKAAKEHLVSQEIYGMY
                     KDRQAVFIYDADKHQLADNAEALYFRIQNDSQNTILQMTLDQEPDLGKKCLMTIDAKG
                     LSGIETSYSVEVVKMDESKQTLWLFDQTGKTGFVMFYRF"
     gene            3183..>4587
                     /gene="dacB"
                     /locus_tag="PKY68_06280"
     CDS             3183..>4587
                     /gene="dacB"
                     /locus_tag="PKY68_06280"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOH24677.1"
                     /translation="MRFFCVFCLLCICSFRLGAEKSIQLRADEYPGTSLAYVVQEVQS
                     GELPAEMNSAQALVPASLSKLLTTGAALEILGPEKRFKTELAYRGLLEKGSLKGNLYI
                     LGGGDPSLGSEYTEEQAGLFLQDWVQAVKQLGISRIEGDIVADPSLFDEEAVSPYWLW
                     EDMGNYYAAGVYGLAVFDNMFRLGLKSGLPGTKPEILYLKPALPEMRIINNLIAKDNA
                     KDSAYFYGVPYRWERVLYGSLPAGREEFVIKGDIPDPPAYLAFRLQQELVKAGIDVQG
                     RALSKQEPGSVCKEPGFFPKEYVNSVNNPQADHEAVVFYTGYSKPLGELVKIINYRSH
                     NVYTEYLLRHLALAAGAKAPVSAREGLEALQRFWSGKGVDISAWQLYDASGLSPLNRL
                     DAASLAQVLRYMALESPQAGLFFSSLPQASLDGTVANFGKTLPGKLRIKSGSMKSVTN
                     YAGYWRLEDKTYAVVLLC"
BASE COUNT         1232 a          989 c         1189 g         1177 t
ORIGIN      
        1 gctaagcaag cttacgaggg caggctaaaa acgctcgttt tactggttgc ttttcaggac
       61 aaggcttttg tttctccgac ggcgcacgat gatttcgaca gactaagtaa cgaattgggt
      121 tacagcgata acggtgcaac cggttcggta agagattatt atcgggacaa ttcaaacgga
      181 gtttttgacc cggaatttgt tgttatgggg ccctataccc tgtcaaaacc catgacttat
      241 tacgggggca ataacgagga aggtaacgac aacgatccca ggcgcatggt gatagaagca
      301 gcctggatgg ccaacgagga aggtgtggat ttctctgagt ttgaccagaa caacgacggc
      361 tatgttgatt tcatctatgt gtattatgcc ggatacaacg aagccgaagg cggtcctgcc
      421 gccagtatat ggccgcattc ctggtcggtt gcctatatgg acagtacact attggacgga
      481 gtaaaattgg gctattacgc ttgttcttca gaattagcag gcgcatcggg ttcgacgatg
      541 tccgccattg gaaccgtttg tcatgaatac ggacataccc tgggtttgat ggatgtgtac
      601 gataccgatg gagcagaaag tggaggaatg tcgggcggct tgggtgcatt gagtttgatg
      661 agttcgggaa attacaacaa taagagtcgt actcctccgc atctgacttc ggaagaacgc
      721 tggtctttgg gctggatgac ccctacaacg atcaccgaag ccggagacta ttccttggct
      781 tccatagatc agaatcaggc ttatataata cctactttaa acgaaaacga gtattttctt
      841 cttgaaaacc ggcagcataa taattgggat ctttacatca aaggcgaggg cctgttgatt
      901 taccatgtgg ataaatcgaa aaacatggta cttggtaaaa cagccttgga gagatggtat
      961 accaacaccg tcaatgtata tccccaacat caatgtatgg atatggaaga agccgtaggc
     1021 tccgaatcca agggtgacat gtatgccttc taccccggtc cttcgggctt gtatactgct
     1081 tttacccgta ccaccactcc ggcagcttta gcctggtcgg gattaccatt gggcaaagaa
     1141 ctttacgata taagattgga aggtcaagaa attcacttca gagtggaact cgaagaccag
     1201 gtctatttcg gagggcaggt aatcaacaga gaaggccaac ctgttcaggg agcacttctc
     1261 tatcttaaag agctcgttga gcagccggtg tcgggcaata cgaccggttt acgttccgcc
     1321 atgcatattt caggtgttta ttataccact acgagcggca tcgacgggcg ttttattttt
     1381 gacgaagact tagatccggg cgtgtatgaa cttttatgtc agtcggaggc atatatcagt
     1441 aattctcaga ttgttgaatt aaaaccaggc agtaacagcg tcttgattac tttgcttacc
     1501 gaggccgaac aattgataga aggaacttta agttggtgcc gggatttcag ttacggagga
     1561 gctatcggaa acgaagggga atctttcgtg gcgggtgcct cctggacggc cgaagagctg
     1621 agtcagtacc ggggaatgta tatagggaga atgacctttt tcattgccgg tggtaatccc
     1681 gatgttgagg ttagcatcta tgccgacagg aatccgatat ttaagaaaaa cattgctgca
     1741 gaagacttga ctgccaatag tttttacagc ctgaatttaa tggccgacag tttggtcata
     1801 aaccccgaaa cagaattaat tcttgcttac caggttagta attaccccaa aaacgcttat
     1861 ccggccggaa tttgcggtgg aagcagagta gagggtaaag gaaatttaat ctcgctggat
     1921 cagggagcta cgtggacaac actatatcag gaagcagaaa ttgaaggcaa ttggttgata
     1981 tctttcgata tatatcgtgg ttcggatttt gttgcaatgg aatctttcaa tctggatccc
     2041 acagacttga ccatgaaaat cggagaaacc cggtatttat acggtcaaat aaaacccgcg
     2101 aatgccacca acgggaaaat aagctggtcg agttcggatt cttccgtggt ttgggtggat
     2161 caaacgggta aagtctttgc tcttgcggcc ggtcaatcgg tcgtaacggc aacaacagac
     2221 ggaggaaatc gttctgccac ttgccaggta aaagttgttc ccaccattgc ctccagtcta
     2281 aacgttaacg tagcgcaaca tgtgtttttt gtggattggg acgaggcgac cggagatgcc
     2341 tggacttttt cctggaggaa tcttggcgat tccgtatatc aagataccac acttactcaa
     2401 agttcttttc tattggaagg cctcgacccc aaaagcacaa cggaggtttt ggtgcaggct
     2461 tattccgaac agcaattggt ggatgaacac gcctttagcg ttactacggc caacaaaaaa
     2521 tcgaatttca tggccatcta tacaacggaa actttctctc ccggacaaaa actattgctc
     2581 gatgtgttga acttgccgga agacgcctct gtaaatgtgt ggaaactcaa cgacgaagtc
     2641 gtttcgggct ctattctcga gttgtccgaa ggcatacatg aactaagggt agaaataact
     2701 acctctaaag gcatagaaat aatacgccgg caattaaaag ttgaataata tgagaaggta
     2761 ttttagtata ctggcactta tcctgttgac cggcttgctt tacctggctt gcgtaaggga
     2821 agaccctttg ttgaaggctg ccaaggaaca tcttgtaagc caggaaatct atggtatgta
     2881 caaagaccgg caagccgttt ttatttacga tgccgataaa catcaattgg ccgacaatgc
     2941 ggaagctctt tatttccgaa tccaaaacga cagtcaaaat acgatactgc aaatgactct
     3001 tgatcaagag ccggatttag gaaaaaaatg cctaatgact attgatgcta aaggtcttag
     3061 cggaatagaa acttcttatt cggtagaggt tgtaaaaatg gacgaaagca aacaaaccct
     3121 gtggttgttc gatcagaccg gcaaaacagg ctttgttatg ttttacaggt tttaaaactt
     3181 agatgaggtt cttctgcgta ttttgtttgc tttgtatctg tagcttcaga ctcggcgcag
     3241 agaaatcaat acagcttaga gcggatgaat atccgggaac aagcctcgct tacgtggttc
     3301 aggaagttca gtcgggcgag ttgccggccg aaatgaattc cgcacaggct ttggttcccg
     3361 cttcgctgag caaactgttg actacgggcg ctgcgctgga aatattgggg cccgaaaagc
     3421 gtttcaaaac ggagctggcc tacagaggat tattggaaaa gggcagcctc aaagggaacc
     3481 tttatatact gggtggaggc gatccgtcgc tgggatcgga atacaccgaa gaacaagccg
     3541 gactttttct ccaagactgg gttcaggcgg tgaaacagtt gggaataagc cgtatcgagg
     3601 gcgatatcgt tgccgacccc tccctttttg acgaagaagc cgtctcgcct tactggctgt
     3661 gggaagatat gggcaattat tacgcggccg gagtgtacgg cctggcggtc tttgataata
     3721 tgttccgtct cggactgaaa tcgggcttgc ccggtactaa acccgagatt ctctatctta
     3781 aaccggcatt gcccgagatg cggataatca ataatctaat tgccaaggac aatgccaaag
     3841 acagtgctta tttttacggg gttccctatc gttgggagcg ggtattgtac ggcagcctgc
     3901 cggccggaag agaagaattc gtcatcaagg gcgatattcc cgatcctccc gcttacctgg
     3961 cctttcggtt gcaacaagaa ttggttaagg cgggaatcga cgtgcaaggt agagccctta
     4021 gcaagcaaga acccggctct gtctgcaagg aacccggctt tttcccgaag gaatacgtta
     4081 attccgtaaa taatccacag gctgaccatg aggccgtcgt tttttatacc ggctattcca
     4141 aacccctggg tgaactggtg aaaataatca attaccgcag ccataatgtg tacacggaat
     4201 acctgctgcg acatttggcg ctggctgccg gagcaaaggc tccggtctct gcccgggagg
     4261 gactggaagc cttacagcga ttttggtcgg gtaaaggcgt ggatatcagt gcctggcaat
     4321 tgtacgatgc ttcgggtttg tctcctctga acaggctgga tgcggcaagt ctcgcccaag
     4381 tgttgcgcta tatggccctg gaaagcccgc aggccggtct gtttttcagc agtttgcctc
     4441 aggcttccct ggatggcacg gtggccaact ttgggaaaac attgccggga aaactgcgta
     4501 tcaaaagcgg atcaatgaaa tcggtaacga attacgccgg ttactggcgg ttggaagata
     4561 aaacctatgc ggtggtcctt ttatgca
//
LOCUS       DAOSGL010000181         4574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_16939_length_4574_cov_4.601018, whole genome shotgun sequence.
ACCESSION   DAOSGL010000181 DAOSGL010000000
VERSION     DAOSGL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16939_length_4574_cov_4.601018"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PKY68_06285"
     CDS             <1..1147
                     /locus_tag="PKY68_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOH24678.1"
                     /translation="TVQNTYTSAQAVAIYHVGDRQTFKNCRFKGFQDTQYLKKGRRSF
                     YYNCLIEGGTDFICAGGTAYYYQCVIKSLKGGYYITAPEDITHSVRLSTGKNLYYGFI
                     FKDCQLQAEEGVPAGSVYLGRPWQENSGAVYLQCRLGDHINPAGWSTWSGDNHLSSSF
                     AEYHSLNAEGTAEADVSGRVDWSIQLSDKDYYDHLGLNKIFNMQTAYQPLPLVITPAA
                     VQNLRVSNNLLSWSAVENAAAYAVYLNSDLIGFSDTTLYADMVYRSGAKNYTVRALGA
                     QGQLGLPAGQSDTVTVALLDSLLNPVAPQEPDALFYPDFTASISLQNGVLSFAAPTGY
                     WIHSLDGQLIKTQQNVREASLYSIKRGVYILTTRDALNRVSRIKFQW"
     gene            complement(1243..3120)
                     /locus_tag="PKY68_06290"
     CDS             complement(1243..3120)
                     /locus_tag="PKY68_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH24679.1"
                     /translation="MRKFLFFSALFFVSSFVSLAESNTSVKNDTTLEISPVTIVSNKP
                     ADLTNVSRVGIQTIENRYEPSVLSLLSEEVPGLFTTQRGVMGYGVAGGAAGTINIRGL
                     GGNPTSQVLMLIDGQPQYMGLMGHPLADTYLCAMAEQITVNRGPASVLYGSNALGGTV
                     NINTAIPRQGIHGKAQVMYGSYNSLNTLVSNSIRKGKFHSFASLSYDRTDGHRENSDF
                     ERYAGFVKLGYELNSNWRISSDLNLTRFEASNPGTTADPMIENDASIFRGMFNLNLGN
                     NFRKSSGTVQAYLNFGKHFINDGYIDGFGAPRPSRFNSKDRLAGLSAHQNFSLFDGNS
                     LSTGLDLQQYGGEAWNTAAADGSRLAELADTALYNLAVYLTTRQQLGDHISIHAGLRA
                     DKNQLFGVEWIPQAGIDFKLNTGTQLKALFGKGFRNPTIRELFMFVPKNPDLQPERIL
                     HYELAVSQTLLEQKINMELNLFYMTGDNSIQMVYQPDESRMKYVNTGEIENWGLELSA
                     HYALNRRLSFNGNYSWLHMENPVTGSPKHKLYLAALYRQKSWSISTGLQYINGLYTQT
                     GNSPETQNFALWNVRGSYRLLSWMEIFLKGENLLNQSYEINKDFPMPGITVFGGFRLN
                     F"
     gene            complement(3244..3645)
                     /gene="nikR"
                     /locus_tag="PKY68_06295"
     CDS             complement(3244..3645)
                     /gene="nikR"
                     /locus_tag="PKY68_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOH24680.1"
                     /translation="MALKRFGVSLDEQLLQSLDQFVTEFGFANRSQAIRFLVEKNIAE
                     KKWKCNNIVAGTVVLMFENEKKEIVALIDAIQEEYQDIVLSSSHYYLNSAIHLVVVTI
                     KGKAVRLTEFSDKLITIKGIKHGKLAMSRAE"
     gene            complement(3720..>4574)
                     /locus_tag="PKY68_06300"
     CDS             complement(3720..>4574)
                     /locus_tag="PKY68_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOH24681.1"
                     /translation="RRAELRPEPYLIAEILAANAGSAMTITGNPQNMLVGISSQIPYA
                     EFLLRLLPVSLLSMLLIEGTMRLFYRKEFADKAKLPAENETDTQQKYCWRSGAIFLLV
                     MAGFFGGHYLNLSIPMIALTGAALILLAGKAKPSQVVQKVDWVLLLFFASLFIVVEGV
                     QKTALMQGFLKAELLTDDAAGLGWLHGISLVASQIVSNVPFAVLMLPLLQAAQSDMLW
                     LGLASASTLAGNATIIGAMANLIVIESAEKYGVRLGFMRFFRVGIVVTLLSLILSFVL
                     LYAEYLFF"
BASE COUNT         1201 a         1080 c         1108 g         1185 t
ORIGIN      
        1 aacggtgcaa aacacctata cttctgccca ggcggtggcc atttaccatg tgggcgatcg
       61 ccagacgttc aaaaattgcc ggtttaaagg ttttcaagat acccaatacc tcaaaaaggg
      121 cagacgttcc ttttattata attgcctgat cgaaggcggc accgacttta tttgtgcagg
      181 tggtaccgct tattattatc agtgcgtcat caagagcctg aagggaggat attatattac
      241 cgctcccgaa gatattaccc attcggtaag actttctacc ggaaaaaacc tgtattacgg
      301 ctttattttc aaagattgtc aactccaggc cgaagaggga gtgcctgccg gttccgtgta
      361 tctgggccgg ccctggcagg aaaacagcgg agccgtgtat ctgcaatgcc gccttggcga
      421 ccacattaat ccggccggct ggtccacatg gagcggagac aaccacctga gcagctcttt
      481 tgccgaatac cacagcctta atgcggaggg cacggccgag gccgatgttt cgggacgggt
      541 agactggagc attcagttga gcgacaagga ttattacgac cacctgggat tgaacaaaat
      601 ctttaatatg caaactgctt accaaccttt gcccttggtc ataactcccg ccgcggttca
      661 aaacctccgg gtgagcaaca atctgctcag ctggtctgct gtagaaaatg cagcggctta
      721 tgccgtctat ctgaattccg acttaatagg cttcagcgat accacgctgt atgccgacat
      781 ggtttaccga agcggagcaa aaaactatac ggtgagagcc ctgggagctc aggggcagtt
      841 gggcttgccg gccggacaaa gcgatacggt cacggtagcc ttgctcgaca gcttattgaa
      901 tcccgtggct ccccaagagc ccgatgccct gttctatccg gattttacgg caagtatttc
      961 cctgcaaaac ggagtattga gttttgcagc tcccaccggt tattggattc attcgctgga
     1021 cgggcaattg atcaagactc aacagaacgt cagggaggct tctttgtatt ctataaaaag
     1081 aggagtttat atattgacaa ccagggatgc tctcaatagg gtcagccgaa taaaatttca
     1141 atggtagcca gtgtcttaga agtacctaac tgcttctaag acaccttgct ttctgtttga
     1201 ttgaacaact gttttgtaat tttcagagtc ggaactccgg aatcaaaaat tcagtctaaa
     1261 gcctccgaac accgtgatac cgggcatggg aaagtccttg ttgatctcgt acgattgatt
     1321 gagcaggttc tcgcccttaa gaaagatttc catccatgat aagagccggt aactgccccg
     1381 gacattccaa agagcgaaat tttgcgtttc gggactgttt cctgtttgag tgtaaaggcc
     1441 gttaatatat tgcagcccgg ttgatatact ccaggatttt tgccggtaga gcgccgccag
     1501 atagagtttg tgtttaggac tgccggttac gggattttcc atgtgtagcc agctgtaatt
     1561 tccattaaag ctcaagcgcc ggtttagggc ataatgggcc gacaattcca gaccccagtt
     1621 ttcgatttca ccggtgttga cgtatttcat gcggctttcg tccggctggt aaaccatttg
     1681 tatgctgttg tcgcctgtca tataaaacag attcagttcc atgttgattt tttgttccag
     1741 caaggtttga gacacggcca attcataatg aaggatgcgt tccggctgca agtcgggatt
     1801 tttgggaacg aacataaaaa gttccctaat ggtgggattg cgaaaacctt tgccaaacag
     1861 ggctttcagt tgggttcccg tattcagttt aaagtctatg cctgcctggg gtatccattc
     1921 cacgccgaat aattgatttt tgtcggctcg caaaccggcg tgtatggata tgtgatcgcc
     1981 caactgttga cgggtggtca ggtacactgc caggttgtag agggcggtgt cggccaattc
     2041 tgccagccgg ctgccgtcgg ccgccgcagt attccaggct tcgcctccgt attgttgtaa
     2101 gtccagaccg gtacttaggc tgttgccatc gaacaaacta aaattctgat gagccgataa
     2161 tcccgccagc cggtcttttg agttaaaacg ggaaggccgg ggagctccga aaccgtctat
     2221 gtatccgtcg ttaataaaat gcttgccgaa attgagatag gcctgtaccg tgcctgagct
     2281 cttgcgaaag ttgtttccca ggttcagatt gaacatgccc ctgaaaatgg aagcatcgtt
     2341 ttcgatcatg gggtctgctg tggtgccggg gttggaagct tcgaaccggg taaggttcaa
     2401 gtcggacgat atcctccagt tggaattcag ttcgtaaccc agtttgacaa aaccggcata
     2461 gcgttcaaag tctgagtttt cacgatgtcc gtcggttcgg tcgtaactca gtgatgcaaa
     2521 ggaatgaaat tttcctttgc ggatgctgtt gcttaccaga gtattcaggc tgttgtagct
     2581 gccgtacatc acttgtgcct tgccgtgaat gccctgacgg gggatagccg tgttgatatt
     2641 gactgtgccg cctagagcgt tggaaccgta aagtaccgaa gccggccccc tattgacggt
     2701 gatttgttcg gccatcgcac acaaataagt gtctgccagt ggatgcccca tgagacccat
     2761 gtattgaggt tgtccgtcaa tcagcatcaa cacctgcgaa gtgggattgc cacccagtcc
     2821 ccggatattg atggtgccgg ctgcgccccc cgccacaccg taacccatta ctccccgttg
     2881 cgttgtgaat aagcccggaa cttcttcgga caagagcgat agcaccgagg gttcgtaacg
     2941 gttttctatg gtctgaattc cgacacgact cacattggtt aagtctgccg gtttgttgga
     3001 cactatggtt acagggctga tttctaaagt ggtgtcgttt ttaacgctgg tattactctc
     3061 ggccagtgac acaaaagacg acacaaaaaa cagagcagaa aaaaacaaaa atttcctcat
     3121 agagcaataa gttaagaata aaaagtgaca cgatttaagt aaaaagtgtc acaaaggaag
     3181 acaaaaaagc tgtaaccgca aaaataccgg cgtgaaaaat gttaaggaaa gaaagaatat
     3241 ggtctactcg gcgcggctca tggccaattt tccgtgtttt attcccttta tggtaatgag
     3301 tttatcggaa aactcagtca gcctcacggc tttgcctttg atcgtaacca ccaccaagtg
     3361 gatggcgctg tttaagtaat aatgagaaga agaaagtaca atatcttggt attcttcttg
     3421 tatggcatca ataagggcaa ctatttcttt tttttcgttt tcgaacatta aaaccactgt
     3481 tccggcaact atgttgttgc atttccattt tttctcggca atatttttct ccaccaaaaa
     3541 acgaatagcc tgtgaccgat tagcaaaacc aaattcagta acgaattgat ccaacgattg
     3601 gagtagctgt tcgtcgagag aaacaccaaa acgttttaga gccataactc agtgtttatt
     3661 tgtaacaaag gtaaataaaa ccttcgatat taggagatgt ttattggaac ggcctgagtt
     3721 tagaaaaaga gatactcggc gtatagcaac acaaacgaaa gaatcagact caacagagtc
     3781 acaacaattc ccactctgaa aaaacgcatg aatcccaaac ggacaccgta tttttcggcc
     3841 gattcaataa caataagatt ggccatggcg ccgataatag tggcgttgcc cgccagtgtt
     3901 gaggccgaag ccagtcccag ccacagcata tcactctggg ctgcttgcag caagggcagc
     3961 atcaaaacgg caaaaggcac attgctgact atttgcgagg ccactaggga aatcccgtgc
     4021 aaccagccca gtccggccgc gtcgtcggtg agcagttcgg cctttaaaaa gccttgcatc
     4081 aaggctgttt tttggactcc ttctaccaca ataaacaaag aagcaaaaaa cagcaacaac
     4141 acccagtcga ctttttgaac aacctgtgaa ggttttgctt tacctgccaa taatatgagc
     4201 gcagcgccgg tcagagcgat catcggaatg gataagttaa ggtaatgccc tccaaaaaag
     4261 cccgccatca ccagcagaaa aattgcaccc gatctccaac agtatttctg ctgtgtatct
     4321 gtttcgtttt cggccggcag ttttgctttg tcagcgaatt ctttacggta aaacaaacgc
     4381 atcgttccct cgatcaacaa catcgaaagc agtgacacgg gaagcagccg gagcaggaat
     4441 tcggcgtaag ggatttggga actgattccc accagcatat tctgtggatt tcccgttatg
     4501 gtcatggcgc tgcctgcgtt tgccgccaaa atttcggcaa tgagataagg ctccggcctg
     4561 agttccgctc tccg
//
LOCUS       DAOSGL010000182         4558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17023_length_4558_cov_4.251832, whole genome shotgun sequence.
ACCESSION   DAOSGL010000182 DAOSGL010000000
VERSION     DAOSGL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17023_length_4558_cov_4.251832"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PKY68_06305"
     CDS             complement(<1..747)
                     /locus_tag="PKY68_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOH24682.1"
                     /translation="MWVEQYIQYLRYEKNYSSHTVFAYKRDLEQFLAFASDTFQLEHP
                     SDIDADIIRAWMLALMEKGDTARSVNRKLSSLRSYWRYLLRQGLVTSNPTQKVLTPKI
                     KKALPSFLKEEELNDLLDKAREEGQTDSFTESRDNLILEMFCLTGIRRAELIGLKTGD
                     VDIGSASLKVTGKRNKQRILPFGTALKKSIENYLVLRSDYLDKLEDYPEVEPPFFINA
                     KGRALNPRRVYSLVNKKLAEVGNLSQKSPHV"
     gene            complement(844..1035)
                     /gene="rpsU"
                     /locus_tag="PKY68_06310"
     CDS             complement(844..1035)
                     /gene="rpsU"
                     /locus_tag="PKY68_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOH24683.1"
                     /translation="MIIVPVKEGENIERALKKFKRKFEKTGTVKELRNRQAFRKPSVV
                     KRQQKMKAVYVQQLQQNEE"
     gene            1231..3018
                     /locus_tag="PKY68_06315"
     CDS             1231..3018
                     /locus_tag="PKY68_06315"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOH24684.1"
                     /translation="MYNSIPKRLAALRNLMETNAIEACIIPTSDSHQGEYVSSHFKFR
                     KYLSGFTGSAGTLVVGKDKAGLWTDSRYYLQAEQELRGSGIDLFKTTLPETPGIPEWI
                     AEMNYRSAGIDGEVFAAREVLKMIQVFNKHGVRLVDDFKPYTRVWPDRPDLPDSPFYV
                     FPVGYAGKSVKEKLSELRAELSRQGADYLPVSGLDDIAWLFNLRGSDVDFNPVGLAFA
                     LIGPDIANLYTDKNKIGESTLAYLKENNIQLRDYSALYADLSKLPAGSGVFTDTARIN
                     YFIYKHIPSNCRRVEGTAPTTTLKSIKNPVEIAGFRTAMQKEGVALVRFWRWFEAALE
                     QGKEFYETDLVRKMSEFRLEQDHCKGDSFYSIISYNEHGAIVHYSPSESTKARILPDG
                     YLLVDTGGQYLEGTTDITRTFSLYRGATPEEFKQDYARILKGNIALSECIFPTHTRGS
                     QLDIMARQFLWKDLLQYGHGTCHGIGHFLNVHEGPQSIRMEENPELIKAGMVMSNEPG
                     IYRAGKYGLRIENMMLVIEKGESEFGQFLGFETLSLCPLELKAIEKKYYTGQEIDWIN
                     AYHRFVYNRLSAFLSEEEKAWLALKTKEV"
     gene            3020..3538
                     /locus_tag="PKY68_06320"
     CDS             3020..3538
                     /locus_tag="PKY68_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOH24685.1"
                     /translation="MALIKSVRGFDPVIGPNCYLAENASIIGDVVLGEGCSIWFNTVL
                     RGDVNSIRIGNNVNIQDGSVLHTLYQKSVIEIGDNVSIGHNVTIHGAKIEKLALIGMG
                     AVVLDHAVIGEGAIIAAGSVVLSGTRVEPYSVYAGVPAKFVKFADAEQTNTINKRISD
                     NYRFYASWYEEE"
     gene            3722..>4558
                     /locus_tag="PKY68_06325"
     CDS             3722..>4558
                     /locus_tag="PKY68_06325"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOH24686.1"
                     /translation="MQKNLVIVESPAKAKTIEKFLGKDFKVMSSFGHIRDLRKKDLGV
                     EIENNFKPVYEISPEKKKLVGELKSAAQKAETIWLASDEDREGEAIAWHLSEVLNLKE
                     ENTKRIVFHEITKEAIENAIKKPRNIDLNLVNAQQARRVLDRIVGFELSPVLWKKIKP
                     ALSAGRVQSVAVRLVVEREREIQQFNSESFYKLSLVFLVPNVDGSITELKAEFPEKIE
                     QKETALALLELLKTATFKVEDISTKPLRRSPAPPFTTSTLQQEAARKLGFSVAQTMRV
                     AQS"
BASE COUNT         1334 a         1037 c         1016 g         1171 t
ORIGIN      
        1 cacatgcggg cttttttgag acaaattgcc cacctctgct aattttttat tgacaagact
       61 atatacccgg cgaggattca gagcccgtcc tttggcattg ataaaaaagg gcggctccac
      121 ttccgggtaa tcttcgagtt tatccagata atcgctgcgt aacaccaaat aattttctat
      181 tgactttttt agagcggttc caaagggcaa aatacgctgt ttattgcgct ttcccgtaac
      241 cttgagcgaa gccgagccta tgtctacgtc tccggtttta agaccaatta attcggcccg
      301 ccggataccc gtaagacaaa acatctctaa aatcagatta tcacggcttt cggtaaagga
      361 atcagtctgt ccttcctccc tggctttatc gagtaagtcg tttaattcct cctcttttaa
      421 aaaagaagga agagcctttt taattttggg tgtcagcact ttttgtgtgg gattggatgt
      481 cactaaaccc tgacgcaaca gataacgcca atacgaccgg agagaagaaa gtttgcgatt
      541 cacggaacgg gcagtgtcgc ccttctccat taaggcaagc atccatgctc tgataatatc
      601 ggcgtcgatg tctgagggat gttccagttg aaaagtgtcg gaagcaaagg caaggaattg
      661 ttccaaatcg cgtttatagg caaaaacagt atgagaagaa taatttttct cataccgtaa
      721 atattgtatg tattgctcta cccacacagg cgctgcttga aaaattgctc cgcaacgaat
      781 atagcaaagc tatttcagaa caacaaaaaa acagacctaa ttttaagtgt ttttctgtct
      841 gttttactct tcgttttgtt gcaactgttg aacgtaaaca gccttcattt tttgctggcg
      901 tttgacaacc gaaggctttc tgaaagcttg tctgtttctt agttctttaa cggttcctgt
      961 tttttcgaat ttacgtttga acttcttcaa agccctttcg atgttttcac cctctttgac
     1021 gggaacaatg atcataatcg tgttttgtat aaaatgattt taattcggcc tgcaaaagta
     1081 gacaaaacaa atcagtaaac aaaaaaaact cagaatcttt cgaaaatatt ttatgtttac
     1141 tacccaaaat tcaattaaga gccggaagtt aaaaagctgt ttttttacta cctttgcact
     1201 tttactaaac cgaccatata ccttaatata atgtacaaca gcattccgaa aagattggcg
     1261 gcattacgaa atctgatgga gaccaatgcc atagaagcct gcataattcc aacctcagac
     1321 agccatcagg gagaatacgt ttcctcgcat tttaaattca gaaaatacct ctcggggttt
     1381 acgggtagtg ccggaactct ggtagtaggc aaggacaaag ccggcttatg gacagactcc
     1441 aggtattacc ttcaggcgga acaggaactc aggggcagtg gtatcgattt gtttaaaacc
     1501 accctgcccg aaactcccgg cattcccgaa tggatagctg aaatgaatta tcgttcggcc
     1561 ggtatcgacg gagaagtatt cgccgcccgc gaagtgctca aaatgatcca ggtcttcaat
     1621 aaacacgggg taagattggt cgacgatttc aaaccttaca cccgggtctg gcccgacagg
     1681 ccggatttac cggattcccc tttttatgtt ttccctgtcg gctatgccgg caagagcgta
     1741 aaagaaaaac tttcggaact cagagccgag ctcagtcgtc agggagcaga ctatctgccc
     1801 gtcagcggtc tggacgatat cgcctggctg tttaacctaa ggggcagtga cgtggatttc
     1861 aatccggtag gtcttgcttt tgccctgatc ggcccggaca tcgcgaacct atacaccgac
     1921 aagaacaaaa taggcgaaag cacgctggcc tatctcaaag aaaacaatat tcaactacgt
     1981 gactattcgg ctctgtatgc agatttaagt aaacttccgg caggttccgg agtctttaca
     2041 gacacggcac gtatcaacta tttcatttac aaacatatac cctcaaattg ccgccgggtg
     2101 gaaggtactg ctcccacaac aactctgaaa tcaatcaaaa atccggtcga aattgccgga
     2161 tttcgtaccg ccatgcaaaa agagggggtg gccttggttc gtttctggcg ctggttcgaa
     2221 gctgctttgg aacaaggaaa agagttctac gagaccgacc tggtccgcaa aatgagcgaa
     2281 ttcagactgg aacaggatca ctgcaaagga gacagtttct attccatcat tagttacaac
     2341 gaacacggag ccattgttca ctacagtcct tcggagagta ccaaagccag aattttgccc
     2401 gatggttacc tactggtaga taccggaggc caatacctcg agggcactac cgacattacc
     2461 cggacttttt cactctaccg gggagccact cccgaggaat tcaaacagga ttatgcccgc
     2521 attctcaagg gcaatattgc cctgtccgaa tgtatattcc ccacccatac cagaggctct
     2581 caactggaca ttatggcccg ccagtttctc tggaaagacc tgcttcaata cggacacggc
     2641 acctgtcacg gcatagggca tttcctaaat gtgcacgaag gcccccagag catacgcatg
     2701 gaagaaaatc ccgaactgat caaagccggt atggtcatgt ccaacgagcc cggcatttac
     2761 cgcgccggaa aatacggtct gcgtatagag aatatgatgc tggtaattga aaaaggagaa
     2821 tccgaattcg ggcagttcct gggtttcgaa acccttagtc tgtgtccctt ggaacttaag
     2881 gccattgaaa aaaaatatta taccgggcag gaaatcgact ggataaacgc ttaccaccgt
     2941 tttgtctaca accggctttc agcctttctg agcgaagaag aaaaagcctg gctggctcta
     3001 aaaacaaagg aagtataaca tggcattaat aaaatcagtc aggggcttcg accctgtaat
     3061 cgggccgaat tgctatttgg ccgagaacgc aagtattatt ggggatgtgg tattgggcga
     3121 gggctgcagc atctggttca atacggttct acggggagac gtcaattcca tacgtatagg
     3181 aaacaacgtg aatatacagg acggctccgt gctgcacacc ctgtatcaaa aatctgtcat
     3241 agaaatcggg gacaatgttt ccatcggtca caacgtcacc atacacggag ctaaaattga
     3301 gaaacttgca cttataggaa tgggggctgt agtgctggat catgccgtca tcggagaggg
     3361 ggccataatt gcagcaggtt cggtagtgct cagtggaacc agggttgaac cctacagtgt
     3421 ctatgccggt gtcccggcca agtttgtcaa gtttgccgac gcagaacaaa ccaatacgat
     3481 caataaaaga atttcagaca attaccgatt ctacgcgagt tggtatgaag aagaataagg
     3541 tcttgcatat gctgatttct gcataaattt tcagaaaaat aacccgacac tgaactgttt
     3601 ttactaattt tgcgccgttt ttacagctgt gaattggtga ataattggat ttaagccctc
     3661 ttttttttgt gatgttgaac ttccggataa atagttccga attaattgaa agcgctcttt
     3721 aatgcaaaag aatttagtta tagtagaatc cccggcaaaa gccaaaacca tcgaaaagtt
     3781 tttaggaaaa gacttcaaag taatgtcgag ttttgggcac attcgtgacc tgagaaaaaa
     3841 agatttaggt gttgaaattg aaaataattt caaaccggtc tatgaaattt ctccggaaaa
     3901 gaaaaaactg gtgggcgaat taaaatcggc agctcaaaaa gcagaaacta tttggctggc
     3961 ctccgacgaa gaccgcgaag gtgaagccat cgcctggcat ttgtctgagg tgctcaatct
     4021 taaagaagag aatactaaac gtatcgtatt ccacgaaatc accaaagagg ccatcgaaaa
     4081 cgccatcaaa aaaccccgca acatcgatct caacctggta aatgcacagc aagcccggcg
     4141 cgtactcgac cgcatagtgg gatttgaatt atcgccggta ctttggaaaa agatcaaacc
     4201 ggcactttct gccggaaggg tacaatcggt agctgttcga ctggtcgtgg agcgggaaag
     4261 agaaattcag caattcaatt cggaaagctt ttataagctg agccttgttt ttctggtgcc
     4321 taatgtcgat ggtagcataa cggaacttaa agctgaattt cccgaaaaaa tagaacaaaa
     4381 agaaacggct cttgccttgc tggaattgct taaaacagcc acattcaaag tggaagatat
     4441 cagcaccaaa cccctcaggc gctcacctgc tccacccttt accacctcta ctttgcaaca
     4501 ggaggccgcc cgtaaactcg ggttttcggt agctcagacc atgcgggtag cccaatcg
//
LOCUS       DAOSGL010000183         4541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17109_length_4541_cov_3.655818, whole genome shotgun sequence.
ACCESSION   DAOSGL010000183 DAOSGL010000000
VERSION     DAOSGL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17109_length_4541_cov_3.655818"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            461..985
                     /locus_tag="PKY68_06330"
     CDS             461..985
                     /locus_tag="PKY68_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24687.1"
                     /translation="MKRIIFILHIVLLFIGCKGLFDRKDDELSFVKTPNTSDKIRLDG
                     YYYNYDFVSTHIVTYFFYRNGIVLFWGTTNSIEHFEEILNDEMVVNKIRAHKSSWGLY
                     QLNNDTIITNGLFVYPGELRLISNISKGIILNDTTIMFNSSVKSNNSVRLRNDTLHFK
                     QFSPKPDSTNVFIR"
     gene            complement(1267..1356)
                     /locus_tag="PKY68_06335"
     tRNA            complement(1267..1356)
                     /locus_tag="PKY68_06335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1320..1322),aa:Ser,seq:gct)
     gene            1530..2564
                     /gene="mltG"
                     /locus_tag="PKY68_06340"
     CDS             1530..2564
                     /gene="mltG"
                     /locus_tag="PKY68_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOH24688.1"
                     /translation="MKIKVRLLLTASGLLLLLAVGIIVWIYGVFYRPNVLDGQSRHIY
                     LYEEDDFDSLLQQLKDKKLLRDERSFKKAADYYDYPRQMHSGHYVLEAGMNNKRALQI
                     LRLGLQQPVRIRFHNLRKVEQLAGLLSKQVMADSLSLLEIFYDRVWLDSLGLTAETLP
                     ALFIPDTYEVWWNSSPEKLMQLFSRSYRQFWNEERRAKAEALGLSPVEVSVLASIIEE
                     ESNVKDEWPVIAGLYLNRLRRGMYLQACPTVKFALNDFSIQRVLTEHTEVVSPYNTYL
                     HLGLPPGPIRIPQKSSLLAVLDAEKHNYLYMCAKDDFSGRHYFSSSLAEHNRYAQKYH
                     RALNQRRILK"
     gene            2628..4496
                     /locus_tag="PKY68_06345"
     CDS             2628..4496
                     /locus_tag="PKY68_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOH24689.1"
                     /translation="MVLTLAILFVTAVFFVWGKIRSDIVALCSLLSLVLLGILKPGEA
                     LAGFSNSVVIMMVGLFIVGGAIFQTGLAKSISLRLLKFAGKSEIKLFLLVVLVTVFFG
                     SFVSNTGTVALLLPIVVSMATEANTNSRRLLMPMAYASSMGGMMTLIGTPPNMIINDT
                     LIKAGYGSLSFFSFLPVGLMITAIGIVYLFPVSKILTRKKEKSSKTGSVKTPDQLSKE
                     YQLADNLFRIEVSKNSDVINKKLSELNITENYHISILVVRRKDTQDGKFFKPVINQRN
                     SRLVSADTTLLPDDLLYVFGNFEEVKKFVTDHKLSFLDKSVSETSRRPDFSRDIKFDE
                     IGIAEVVVMSNSKLVNKMVKESGFRTNYNVNILGIKRSREYLIYNVKDEKIHSGDALL
                     VQGTWQDIERLNNNEPDVVVVGQPSIEASKVPLTSKAPIAAIIMIAMVVAMVINIVPP
                     VIAVMLAALAMILTGCFRNVEAAYKTINWESIVLFAAMIPMATAMEKTGASQMISGSI
                     VGGLDSYGPYAVMAGIYLATSFLTMFISNTATAVLFAPIALQSALSMGVSPYPFLFAV
                     TVAASMCFASPFSTPPNALVMSAGRYTFMDYVKMGLPLQIIYMIVMVFFIPLIFPF"
BASE COUNT         1308 a          903 c         1026 g         1304 t
ORIGIN      
        1 tgcaacaagg ctaatagagg atttagacta gagacacact aaatgtaaca ctaccccaaa
       61 aagcttgctg gggtgttttt attattgaag atgacaagat tttactagaa cgttggcagc
      121 cattcaatcc atttagagca tatataaagc aaggtgaaat tcttaatgat acaacttttc
      181 agttaaccaa gatttatcgg atggtcaatg aagaaaaaac ggatgagata gaaataaacg
      241 agatctttca ttttcgggag ttcagtccaa aacccgacag tacaaatgtt tttataaaat
      301 aggctccatt cgcataggct acaacagtga gacaatacgt gataaaactc aaaattgggc
      361 acatgacaac tggttctcga caccgagatt taaaaaatta aacagacccg ggaaatggta
      421 ttgggaattt tgcttttaaa tatcttatgc taaaaaatat atgaaacgaa taatttttat
      481 actacacata gtattgttat ttatcggttg taaaggcttg tttgatagaa aagatgatga
      541 actttcattt gtaaaaactc caaatacaag tgataaaata agacttgatg gctactatta
      601 taattatgac tttgtgtcca ctcatatagt cacttatttt ttttatagaa acggaatcgt
      661 attgttttgg ggaacaacaa acagtataga gcattttgaa gaaattttaa atgatgagat
      721 ggttgtaaat aaaattcgtg cacataagag ttcttgggga ttgtatcagt taaataatga
      781 cacaatcatt actaatggat tatttgttta tcctggagag ttgaggctta tatcaaatat
      841 ttctaaaggg attattttaa atgacacgac aatcatgttt aattcatccg ttaaatcaaa
      901 taatagtgtt cgcttgcgga acgacacact tcatttcaaa caatttagtc caaaacccga
      961 cagtacaaat gtgtttataa gataggctgg catataccgg acagctttct atcaccttcg
     1021 aaactcctaa attgaatatc ggaatattat tatctttgcg cctgaagttt tgagcagagt
     1081 gccgggattt tttgattgga aagttaaacc cgagaaaagc ccggcggaag tgaataagat
     1141 aagaaatgac cgggcgaccg gccgggaaga catccggact tctaatgaaa gaaataagag
     1201 aaaacaagcg gggagatttt ggagaaaaaa caaggctgtc tcttttgaga aacagccttg
     1261 caaagttggc ggaagaaggg ggattcgaac ccccggtacc ctcacgagta cgacagttta
     1321 gcaaactgtt ggtttcagcc actcacccat ccttccttat cttattaacg cgataataag
     1381 cccggttttg aattacaaat gagaattcga tggtggttta tttaacgcgg cgcaaagata
     1441 ggaagagttt tattttattc aaattttctg ccgcgattta ttaaaaaaac agtgtaaatg
     1501 ctccctcggg agcgaaatga cagaaagagg tgaaaataaa agtacgattg ttgttgacgg
     1561 cttcgggttt gttgcttttg ctggctgttg ggattatagt ctggatttat ggtgtctttt
     1621 atcgtccgaa tgtgcttgac ggacaaagcc ggcatattta tctctacgaa gaagacgatt
     1681 ttgacagcct cttgcagcag ctaaaagata agaaactgtt gagggatgag cgttctttca
     1741 aaaaagccgc cgactattac gattatcccc gacagatgca cagtggtcat tatgtgctcg
     1801 aagctggtat gaataataaa agagctttgc aaattctgcg tctgggcctg cagcagccgg
     1861 tcaggatacg ttttcataac ctgcgcaaag tggagcaact ggcaggtttg ctcagtaaac
     1921 aggtgatggc cgattctctg agtctgctcg agatttttta cgaccgcgta tggctcgaca
     1981 gcctcggttt gacggcggaa acgctgccgg ccttgtttat tccggatact tacgaagtgt
     2041 ggtggaacag cagccccgaa aaactgatgc aattattttc gcgcagttac cgacaattct
     2101 ggaacgaaga gcgcagggca aaagccgaag ccctgggact gagtccggtc gaggtgtcgg
     2161 tgttggcttc cattatagag gaggagagca acgttaagga tgaatggccc gttattgccg
     2221 gcctttacct gaaccgtttg cgcagaggaa tgtatttgca agcctgtccc acggtaaagt
     2281 tcgccctgaa cgactttagt attcaacgtg tactcacgga gcataccgaa gtggtttctc
     2341 cgtataacac ctacctgcat ttgggtttac ctcccggtcc gattcgcatt ccccaaaaaa
     2401 gttctttatt ggctgtgctc gatgccgaaa aacacaatta tttgtatatg tgcgccaaag
     2461 acgatttttc gggacggcat tatttttctt caagtttggc cgaacacaac cgctatgccc
     2521 aaaaatacca tagagcctta aatcaacggc gtatcttaaa gtaaaattta ttacttttgc
     2581 cgcttatata actcaggcga ataataaatc atactatgtc ttctcccatt gttcttaccc
     2641 ttgctattct gtttgttaca gccgtttttt tcgtatgggg aaaaatccgt tccgatatcg
     2701 ttgccttgtg ttccttgttg tcgttggttt tactcggaat tctcaagccc ggcgaggcat
     2761 tggccggatt ctccaactct gtggtaatta tgatggtggg gctttttatt gttggaggag
     2821 ccatttttca gaccggtctt gccaagtcta tcagccttcg cttattgaaa tttgccggga
     2881 aaagtgaaat taaactattt ctcctcgtgg tgctggttac cgtgtttttc ggctcctttg
     2941 tgagcaacac gggcaccgtg gcactcctgc tgccgattgt tgtgagcatg gccacagaag
     3001 ccaataccaa ctcgcgccgc ctgcttatgc ctatggccta cgcaagcagt atgggcggta
     3061 tgatgacgct catcggtacg ccgcccaaca tgattatcaa cgatacattg atcaaagccg
     3121 gctacgggag tttgtcgttt ttctcgttcc tgcccgtcgg gttgatgatt acggcaatag
     3181 gtatcgttta tctctttcct gtttcaaaaa tactgacccg taaaaaagaa aaatcgtcca
     3241 aaacgggctc cgtaaaaaca cccgatcaat tgagtaaaga atatcaattg gctgataact
     3301 tgttcaggat cgaggtttcc aaaaattcgg atgtgattaa taaaaaactt tcggagctga
     3361 atattacgga gaattaccat atttctattc tcgtggtaag gcgtaaagat acccaagacg
     3421 gtaagttttt caaacccgtg ataaaccaga ggaacagccg gttagtaagc gccgatacta
     3481 ctttactgcc cgatgacctg ctctatgttt ttgggaattt tgaggaagta aagaagtttg
     3541 taacagatca taaattgagc tttttggata aaagcgtttc ggaaacgagc cggcgtcccg
     3601 atttttcgag agatattaaa tttgacgaaa taggcatagc cgaagtagtg gtaatgtcga
     3661 actccaagct ggtcaacaaa atggtaaaag aatcgggttt cagaaccaac tacaacgtta
     3721 atattcttgg tataaaaaga agccgggaat accttatcta caacgtgaaa gacgagaaaa
     3781 ttcattccgg cgatgctttg ctggtgcagg gaacctggca ggacatcgaa cgcctgaata
     3841 ataatgagcc cgatgtggta gtagtcgggc aaccctcgat agaagcatcg aaagtacctc
     3901 ttacctctaa agcgccgata gcagccatta ttatgatagc catggtcgtt gctatggtaa
     3961 tcaacattgt gcctcctgtg atagcggtaa tgctggcggc tttggcaatg attctgaccg
     4021 gctgttttcg aaatgtggaa gccgcctata aaaccatcaa ctgggaaagt atcgtacttt
     4081 tcgcggccat gattccgatg gcaacagcca tggaaaaaac cggcgcttcg cagatgattt
     4141 cgggaagcat tgtgggagga ttagacagct acggccccta tgccgtgatg gccggtattt
     4201 acctggcaac ctcttttctc accatgttta tcagtaacac ggcgacggcg gtgctttttg
     4261 cccccattgc cttgcaatcg gcgctttcga tgggagtgag tccttatccg tttttatttg
     4321 ccgttacggt ggcggccagc atgtgctttg caagtccgtt ctccactccg cccaatgcgc
     4381 ttgtgatgtc ggcaggaaga tatacattca tggattatgt aaaaatggga ttgcccttgc
     4441 agattatata tatgattgtc atggtgttct ttattccgtt gatatttcct ttttaatctc
     4501 aaacagtgca gagatcttaa acaggcagcg atctcaaaca g
//
LOCUS       DAOSGL010000184         4525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17204_length_4525_cov_3.793960, whole genome shotgun sequence.
ACCESSION   DAOSGL010000184 DAOSGL010000000
VERSION     DAOSGL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17204_length_4525_cov_3.793960"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PKY68_06350"
     CDS             complement(<1..1120)
                     /locus_tag="PKY68_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOH24690.1"
                     /translation="MKNKYRHLIEQSFEFPNEEFSLVEDELQWFNIPLMDIIKQYGTP
                     LRIFYLPKIGQNIQRAKRMFNVAMAKADYEGDYHYCYCTKSSHFSFILEEALKQKIHI
                     ETSSAFDINIIEALYEEGKIDKDKFILCNGFKRPQYIENIQSLIKNGFENTISILDNK
                     FELDLLNENLDRDFKIGIRIASEEEPKFDFYTSRLGIRYNDIIPYYLNNIRQHQHVHL
                     KMLHFFINTGIKDTAYYWNELTKAVELYCELKQYCPELDSINIGGGFPIQVHLDFNFE
                     YEYMAEEIIAQIKNICTHNKVKEPDVFTEFGSFTVGESGAMLYSIVNQKQQNDRELWN
                     MIDSSFITTLPDTWAINQRFIQLAINNWDSEYQRVHLGG"
     gene            complement(1276..1626)
                     /gene="folB"
                     /locus_tag="PKY68_06355"
     CDS             complement(1276..1626)
                     /gene="folB"
                     /locus_tag="PKY68_06355"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOH24691.1"
                     /translation="MDYLLLNEMKFFAFHGVNEQERIVGNTYLVSLKLGLDMSKACQS
                     DQLEDTLNYASVYKQVAAVMKIPCNLIEHLAEKICLQLRNTFPEIQYIEIRLKKCHPP
                     IVGMMQSAEIILER"
     gene            1767..2129
                     /locus_tag="PKY68_06360"
     CDS             1767..2129
                     /locus_tag="PKY68_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOH24692.1"
                     /translation="MSRDKTVSNGVIHNAIAPGTQITGSIIAEKDFRIDGNIDGDIVC
                     QGKVVVGRQGYVKGRMACINAEIVGQVDGKIQASEILTLRATAVVQGEIKISTLIVEP
                     NALFNGTCEMFRKDAPAE"
     gene            complement(2207..>4525)
                     /locus_tag="PKY68_06365"
     CDS             complement(2207..>4525)
                     /locus_tag="PKY68_06365"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOH24693.1"
                     /translation="KELFDLFDRFRYIIPQGSPMTGIGNEFQVASLSNCFVIGIDGEA
                     DSYGAVIKIDEEQVQLMKRRGGVGHDLSHIRPKGTPVKNSALTSTGIVPFMERYSNST
                     REVAQDGRRGALMLTVSIKHPDSESFIDAKMTEGKVTGANVSVKLTDEFMRAATENLP
                     FVQQYPVNASDPDITKEINAKALWKKIVHNAWRSAEPGVLFWDTILNESVPDCYADLG
                     FRTVSTNPCGEIPLCPYDSCRLLAINLYSYVNKPFTTEASFDYELFKKHVALAQRIMD
                     DIIDLEMEKIDRILEKIDSDPEDPEIKRAERILWEKIKAKTAQGRRTGVGTTAEGDML
                     AALNLRYGTEEASDFSEKIHRLVAIEAYRSSINLAKERGAFSIYNYEREENNPYIKRL
                     FNEAPELKEDMKAYGRRNIACLTIAPTGTTSLMTQTTSGVEPAFSLIYKRRRKINPND
                     PGARVDFKDENGDCFEEYIVFHHKFVTWMEAQGYPTAKNYTQEELDEMIAKSPYYKAT
                     SKDVDWMQKVKMQGRIQKWVDHSISVTINLPADVSEELVNQLYIEAWRSGCKGCTVYR
                     DGSRSGVLLDASEKKDNGRLICFPELTPKRPRELIADVLRFQNNKEKWIAFIGMQNGR
                     PYEIFTGIEDDDEGIALPKNVTKGKIIKNFDDHGVKRYDFQFSNRRGYKTTVEGLSDK
                     FNPEYWNYAKLISGVLRYGMPIDQVIKLVGSLELDSKSINTWKNGVERALKNYLPDGS
                     EVQGEKCPKCGNTLVYQEKCAHCVNCDFSRCA"
BASE COUNT         1179 a          926 c         1043 g         1377 t
ORIGIN      
        1 gcccgcccag gtgaacccgt tggtattccg aatcccagtt gttgatggcc agttggataa
       61 aacgttggtt aatggcccag gtgtcgggta gggtggtaat aaaggaactg tcgatcatgt
      121 tccacaactc ccggtcgttt tgttgttttt ggttgactat ggaataaagc atggcgccac
      181 tttcacctac agtgaaggag ccgaattcgg tgaacacatc cggttctttt accttattgt
      241 gggtacaaat attcttgatt tgcgcaatta tttcttcggc catgtattcg tactcgaaat
      301 tgaaatccag atgcacctga atgggaaatc cgcctccgat attaatgctg tccagttcgg
      361 ggcagtattg tttcaattca caatagagtt caaccgcctt ggtcaattcg ttccagtaat
      421 aggcggtatc cttgatacct gtgttgataa agaagtgcaa catctttaaa tgcacatgct
      481 ggtgttgtct gatattgttc agataatagg gaataatatc gttgtagcgg atgcctagac
      541 gggatgtgta aaaatcgaac ttgggttctt cttccgaagc aatacggata ccaatcttaa
      601 aatccctgtc aaggttctcg ttcaacaagt ccagttcaaa tttgttgtcg agtatggaaa
      661 tggtattctc gaagccattt tttataagcg attggatgtt ttcgatatat tggggacgtt
      721 taaatccgtt gcaaagaatg aatttgtctt tgtcgatttt tccttcttcg tacaaagctt
      781 ctataatatt tatatcaaaa gccgaggatg tttctatatg aattttttgt tttagagctt
      841 cttccaggat aaacgaaaaa tgggagcttt ttgtacagta gcaataatgg taatcgccct
      901 catagtcagc ctttgccata gctacgttga acattctctt tgccctttga atattttgtc
      961 ctattttagg taagtaaaag attctcagag gtgtaccata ttgtttgatg atatccatca
     1021 agggtatgtt aaaccattgc agttcgtctt cgaccaacga aaattcctcg tttggaaact
     1081 caaacgattg ttcaatcaga tgtctgtact tgtttttcat ttttacaata atggttcaaa
     1141 atgtaagatc caactgagtt ggattgtaat aatttctcta atccaattaa aatggatttg
     1201 tccattcttt agaaaaaaga gcggcaaaaa tacaataatt tggacaatta caatgaaaaa
     1261 gaggaaaaaa atcaatcagc gttcgagaat tatttctgca ctttgcatca tgcccacaat
     1321 aggcggatgg cattttttaa gacggatttc gatgtattga atttcgggga aggtattacg
     1381 tagttgaagg cagatctttt cggccagatg ttctatgaga ttgcaaggga ttttcataac
     1441 agcggctacc tgtttgtaaa cactggcata attgagtgtg tcttcaagtt ggtcgctttg
     1501 gcaggctttg ctcatgtcca ggccaagctt gaggctgacc aaataagtat tgcccactat
     1561 gcgttcttgt tcgtttacgc catgaaaggc aaaaaatttc atttcgttca gtagcaggta
     1621 atccataatg aggttttgaa agagtcttga ttaatacggc aaaagtacga ttttttataa
     1681 tttgactcag cacaacgaac aaattagata tttaatacta tatttgcaca caatcaaaac
     1741 ggcttcacac aattaaatta acctttatgt caagagacaa gactgtcagc aatggagtta
     1801 tacataacgc tatcgccccg ggaactcaaa taaccggaag tatcattgca gaaaaagatt
     1861 ttcgcataga tggcaacatt gacggagata ttgtttgtca gggtaaagtc gttgttggcc
     1921 ggcaaggata tgtaaagggc aggatggcat gcattaatgc agagattgta gggcaggtag
     1981 atggtaaaat acaagcatcg gaaatactga ctctcagagc tacagccgta gttcaaggcg
     2041 aaatcaaaat ttccaccctg atagtggagc ccaatgcttt attcaacggt acctgcgaaa
     2101 tgtttagaaa agatgctcct gctgaataaa gtattgttgc gataaataaa aaacggctga
     2161 acctataagt ttcagccgtt tttttattag ccgattgcaa agaccactat gcgcaacgcg
     2221 aaaaatcgca attgacgcaa tgggcgcatt tctcctggta aacaagagta ttcccgcatt
     2281 tgggacattt ttctccttgt acttcggaac catcgggtaa ataatttttc agggcacgtt
     2341 ccactccgtt tttccaggta tttatagatt tgctgtcgag ttccagactg cccactagtt
     2401 ttattacttg atcgatgggc atgccgtaac gtaatacgcc ggaaatcaac ttggcatagt
     2461 tccagtattc cggattaaat ttatccgaca aaccctcaac ggtggttttg taaccccggc
     2521 gattgctaaa ctgaaagtca tagcgtttca ctccatggtc gtcaaagttt ttgataatct
     2581 ttcctttagt gacgtttttg ggtaaagcta tgccttcgtc gtcgtcttcg ataccggtaa
     2641 agatttcata aggacgtccg ttttgcatac caataaaggc aatccatttc tctttgttgt
     2701 tctgaaaacg gagtacgtcg gcaatcagtt cccgggggcg cttaggcgta agttccggga
     2761 agcagatcag tcttccattg tccttttttt ctgaggcatc gagcagtaca cccgaacggg
     2821 aaccgtcgcg gtaaacggtg cagcccttac agcccgagcg ccaggcttct atatagagtt
     2881 ggttgaccaa ttcttcgctg acatcggcgg gcaggtttat ggtcacactg atggaatggt
     2941 ccacccattt ttgtatacgt ccctgcatct tgactttctg catccagtcg acatctttgg
     3001 aagtggcttt ataatagggc gatttggcta tcatttcatc cagttcttcc tgtgtatagt
     3061 tcttggccgt ggggtaaccc tgagcttcca tccaggtgac aaacttatgg tggaaaacaa
     3121 tgtattcttc gaagcaatct ccgttttcgt ctttaaagtc tacccgtgct ccgggatcgt
     3181 tggggttgat tttgcggcgg cgtttataga tgagactgaa agccggttct acgcccgaag
     3241 tggtttgagt catcaggctg gttgtgccgg tgggagcaat ggtcagacag gcaatattgc
     3301 gcctgccata ggctttcata tcttctttaa gttcgggagc ttcgttgaac agtcgtttta
     3361 tatagggatt gttttcttca cgttcataat tataaatact gaaagctccg cgttctttgg
     3421 caaggtttat cgaagaacga taagcttcga tggccaccag gcgatgaatt ttttcggaaa
     3481 aatcggaggc ctcttcggtg ccgtaacgca gattcagagc cgccagcata tcgccttcgg
     3541 cagtagtacc cacaccggtt cggcggcctt gggctgtttt ggctttgatc ttttcccaga
     3601 gtatgcgttc tgcgcgttta atttcggggt cttcgggatc ggaatcgatc ttttcgagta
     3661 tcctgtcgat tttttccatt tccagatcga tgatatcgtc catgatgcgt tgagccagcg
     3721 ccacgtgttt tttgaaaagc tcataatcaa aacttgcttc tgttgtaaaa ggtttattta
     3781 cataggaata caggttgatg gccaacaggc ggcaactgtc gtaggggcaa agaggaattt
     3841 caccgcaggg attggtggaa accgtacgga atcccaaatc ggcataacaa tcgggtaccg
     3901 actcgttcaa tatagtatcc caaaacagta ccccgggctc ggccgatctc caggcgttgt
     3961 gaacaatttt tttccacaaa gcttttgcgt tgatttcttt ggtaatatcc gggtcggatg
     4021 cgttaacggg gtattgttgc acaaaaggga ggttttctgt ggcggctcgc ataaattcat
     4081 ccgtcagttt aaccgagaca ttggctccgg tcactttgcc ttcggtcatt ttagcgtcaa
     4141 tgaaggattc tgagtcggga tgtttaatgg ataccgtcag catcagggcg ccacgcctgc
     4201 cgtcttgggc tacttcgcgg gttgaattgg aataacgctc cataaaaggc actatacctg
     4261 tggaggtcag cgcggaattt ttcaccggcg tgcctttggg gcgaatgtgc gaaagatcgt
     4321 ggccgactcc gccgcgtctt ttcatcagtt gaacctgctc ttcgtcaatt ttgatgacag
     4381 ctccgtatga atcggcttct ccgtcgatac caattacaaa acagttggac agcgaggcta
     4441 cctgaaactc gtttccgatg ccggtcatgg ggctgccttg aggaataatg taacggaaac
     4501 gatcgaagag atcgaaaagt tcttt
//
LOCUS       DAOSGL010000185         4517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17259_length_4517_cov_3.549529, whole genome shotgun sequence.
ACCESSION   DAOSGL010000185 DAOSGL010000000
VERSION     DAOSGL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17259_length_4517_cov_3.549529"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1982)
                     /locus_tag="PKY68_06370"
     CDS             complement(<1..1982)
                     /locus_tag="PKY68_06370"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24694.1"
                     /translation="MKKYIYILLGVFLLTIGTTSCGEDFLEVSSPSQVDEDFVFSTPD
                     EAYKVLMGCYEIFRGRSYVHSNGLFYDLIIGGSDSECHPESYSAQQRHVPEGLYAVED
                     FSIDFSNFQRAWRYCYQMANRLAIVIAQIETKPAFQTAWDAQEVSEWTQLYGEAVALR
                     AIAYHELIRFFGDIPYFDIPIYTQDQSDVGCTSRFVIYDDLLDDLKRVEPFMYRLGEA
                     GVNAERITGTFVQGLIGRIALYAGGYQLYRTDFDYGITLERHPNINGEKWNAIYARHP
                     QYLDYYRIAEQYLGECISEGKATLITTDERGYGNPFQRNFQYMMDLQVSPESLFEIAE
                     TQGFQTERPYAFGRPSKGGGSNNFPCKAYGQSRIYASFYYGDFDPLDLRRDASVTVTC
                     NQHPCTEVLLLFDPGSQNKGGLANNKWDESRMSNPYTTRQRQSGINTCYMRMADVILM
                     LAEVYAELGKEGPAKTELSKVRARAFPATAKATKVDAYIAALSGDALKEAIAQERKLE
                     FAGEGLRRYDLIRTGKLPEKIVDLRKAQGAMINGLKTNGYYTFANGNTISAYIYTKPV
                     NTADLGMTYMLTKQCDVLPGDPAYPVKFPSWRGQHDGWEGFDNTSGTRNLAIQGLFEF
                     IDSASVQGKALIAAGYEVVPWGSTIVANEDQYLHDI"
     gene            complement(2002..>4517)
                     /locus_tag="PKY68_06375"
     CDS             complement(2002..>4517)
                     /locus_tag="PKY68_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24695.1"
                     /translation="RDAWEMLWAIGPYAATYGNTPGIDYYKNVVAQNYQRQVYGESFS
                     HSHNLNITGGNDKTNFLVAFNHIDDDGLKVNSWYKRTNASIKLDHKLYKKLTLSLDTR
                     FTHTNSVGNESTTNTRGSVLSSSYFFRPIVTQHVMGELDPMVNSSLGMYDELLQDEFN
                     PVARIKDYTPERNNRSLRANTALNWEIVKGLVARTELGLNTYWNKNHTWTGAIFNDYY
                     DANGNKTYSGNASISRSEGWNMRWANVLSYEIRDLGEAHHVGVTLGQEIMDSNSESMS
                     ISGSRYPASYTPERAFAMMDQYDPGNLAYHSLSGSIGTPSRLMSYFARANYGLLDRYL
                     LTVTFRADGSSRFAPTNRWGYFPAGAIAWRASEENFIKEQDWVDNLKLRFSLGSVGND
                     GISADLWKMNWTTGGLANYSINEERQVIYQPGSDVMANPNLKWETTITRDLGLDFGFL
                     DNRVFGSLDLYYNTTKDLLMLTPISDITGFSFTYDNVGSTSNRGIEVLLGGDIIKTND
                     FNLTASLNVNINRGRVEELAEGIDGLYKTEWGSTMTRPNTGDYILEEGQPVGMVRGYT
                     YEGWYTTSDFDYDPVTGIYTLKDGVPDIAAGVIGTVYGTENNKPGSQTAYPGVIKYAD
                     ISGPDGVPDGVVDESDVVIIGNMNPKHTGGLNLNASYKSFDLGLFFNWSYGNQIYNAN
                     YLATIYGSKNDGLYRNRMDYLAGAYKIYDIKNGNLVKVTDPAALDALNTDASLFLPYH
                     ENAVVSTLGIEDGSFLRLNTATLGYTMPKNMIRKIGLVNARLYASVYNLFTLTKYSGL
                     DPEVNTNTSQGGARYPTTGLDWGAYPRARSFTFGVNLEF"
BASE COUNT         1200 a         1069 c         1032 g         1216 t
ORIGIN      
        1 aagatatcgt gcaggtactg gtcttcgttg gcaacaatgg tactgcccca gggaaccact
       61 tcataaccgg cagcgataag ggctttgcct tgtacgcttg ctgaatcgat gaattcaaac
      121 aaaccttgaa tggccagatt acgagtgccg gaggtattat cgaaaccttc ccagccatcg
      181 tgttggcctc tccaactcgg gaattttacc ggatatgcgg gatctcccgg caagacatcg
      241 cattgcttgg tgagcatata agtcatccca agatcggcgg tgtttacagg cttggtataa
      301 atataagcag atatggtatt gccgttggca aaggtataat aaccattggt tttcagccca
      361 ttgatcatgg ctccctgagc tttacggaga tcaactattt tttcgggcaa tttgccggta
      421 cgaatcaagt catagcgacg caatccttcg ccggcgaact cgagtttacg ttcctgagca
      481 atggcttctt tgagtgcatc accgcttaaa gcagcaatat aagcatccac ctttgtggct
      541 ttggctgttg cggggaaagc tcttgcccta actttggaaa gttctgtttt ggccgggcct
      601 tctttgccca attccgcata cacttccgcc agcatcagga ttacatcggc catacgcatg
      661 taacaggtat tgatacccga ttgacgttgc ctggtagtat agggattgga catacggctt
      721 tcgtcccact tattgttggc cagacctccc ttgttctgcg aaccgggatc gaacaacaac
      781 aacacttcgg tacaaggatg ctggttgcag gtaacggtca cgctggcatc acgacgcaaa
      841 tccaaaggat cgaaatcacc atagtagaac gaggcataaa tacgactctg tccatacgcc
      901 ttgcagggga agttgttgga tccgcctcct ttggagggac gcccgaaagc ataggggcgt
      961 tcggtctgga agccttgggt ttcggctatc tcaaacaagg attcgggact tacctgcaga
     1021 tccatcatat actggaaatt gcgttggaaa ggattgccat aaccacgctc gtctgtggtt
     1081 attaaagtgg ctttgccttc gcttatacat tccccgagat attgttcagc aatcctgtaa
     1141 taatcaaggt attgtggatg acgggcgtag atagcattcc atttctctcc attaatattg
     1201 gggtgtctct ccagagttat cccataatcg aaatcggtgc ggtagagttg ataacctccg
     1261 gcataaaggg caattcttcc gataagcccc tgtacgaaag ttccggtaat gcgttcagca
     1321 tttacacccg cttctcccaa tctgtacata aaaggctcaa cccgtttgag atcgtccaat
     1381 aaatcatcgt agataacaaa ccttgatgta cagcccacat ccgattgatc ctgggtgtag
     1441 atgggtatgt caaaatacgg gatgtcgcca aaaaaacgaa tcagttcgtg ataggcaatg
     1501 gcacgcaaag cgactgcttc tccataaagc tgggtccatt cggaaacttc ctgagcatcc
     1561 caggctgtct ggaaagccgg cttagtttca atttgcgcaa ttactattgc caggcggtta
     1621 gccatttgat agcaatacct ccaagctctt tgaaaattgc tgaaatcgat ggaaaagtct
     1681 tcgacggcat acaaaccttc gggcacatgc ctctgttgtg cggaatacga ctccgggtga
     1741 cattccgaat cggaaccgcc gattatcagg tcgtaaaaaa gtccattgga gtgaacatag
     1801 gatctaccac gaaaaatttc gtaacaaccc atcagaactt tataggcttc atcaggcgtc
     1861 gaaaatacaa agtcttcgtc cacctgcgaa ggagaagata cctccaaaaa atcctctcca
     1921 cacgaagtag tgcctatagt cagcaaaaag acacctaaaa ggatataaat atattttttc
     1981 attgttatac ggcattaata attaaaactc cagattgact ccaaaagtga aggagcgtgc
     2041 acgaggataa gccccccaat ccaaaccggt ggtaggataa cgtgcaccac cctggctggt
     2101 attggtgttg acttcaggat ccagacccga atattttgtc agagtgaata aattatacac
     2161 actggcatac aggcgggcat taaccaaacc tatctttctg atcatgtttt taggcatggt
     2221 ataacccaaa gtagctgtgt tcagacgcag gaaggaacca tcttctattc cgagcgtaga
     2281 aacaaccgcg ttttcgtgat agggaaggaa aagactagcg tcggtattca aagcatccag
     2341 agctgccgga tcggtcactt ttaccagatt cccgtttttg atgtcgtaaa tcttataggc
     2401 accggctaaa taatccattc tgttacgata taaaccgtcg tttttgcttc cgtatatggt
     2461 agccaggtaa ttggcattgt aaatttgatt accgtaactc cagttgaaga acaatcccaa
     2521 gtcaaaactt ttgtaggagg cattgaggtt gagacctccg gtgtgtttgg gattcatgtt
     2581 accgataatc accacgtcgc tttcgtcgac cacaccatcg ggcacgccat cggggccact
     2641 gatgtcggca tacttaataa ctccgggata ggcagtctgg gatccgggtt tattgttttc
     2701 ggttccgtaa acagttccta ttacgccggc tgctatatcg ggcacgccat ccttaagggt
     2761 gtaaatacct gtgaccggat cgtagtcaaa gtcactggtg gtataccaac cttcgtaggt
     2821 ataaccacgg accataccaa ccggttggcc ttcttccaaa atataatctc cggtattagg
     2881 tctggtcatg gtggaacccc attcggtctt ataaagtccg tcgatacctt cggccagttc
     2941 ttctaccctg ccccggttaa tattcacatt taaactggcg gtaaggttga agtcgtttgt
     3001 ttttatgata tctccaccga gaagtacttc aatacctctg ttgctggtac taccaacatt
     3061 atcgtaagta aaggaaaaac cggtgatatc cgaaatggga gtcagcatca acagatcttt
     3121 ggtggtattg taatacagat ccaatgaccc gaacactctg ttatccagga aaccaaaatc
     3181 caaaccaagg tcccgggtaa tggtcgtttc ccatttgagg ttggggttgg ccataacatc
     3241 ggaacctggc tgatagatta cctgcctttc ttcgttaata gaatagtttg ccaatcctcc
     3301 cgtagtccag ttcattttcc aaagatccgc actgattccg tcgttaccta cagagcccag
     3361 ggaaaaacgc agttttaggt tgtctaccca gtcctgctct ttgataaaat tctcttcact
     3421 tgcacgccag gcaatggctc cggcagggaa atacccccag cgattagtgg gagcaaaacg
     3481 ggaagaacca tccgcacgga aagttactgt gagcaggtag cggtcgagca ggccgtaatt
     3541 agctctggca aaataggaca tcaatcttga aggcgttcct atcgatccgg acagagaatg
     3601 ataagccaag ttaccgggat cgtactgatc catcatagca aaggcgcgtt ccggagtata
     3661 agaggccgga tagcggctgc ccgaaataga catgctttcc gagttagaat ccattatttc
     3721 ctgacccagc gtcacaccta catgatgagc ttctcccaaa tcccgaattt cgtaactcag
     3781 gacgttggcc caacgcatat tccaaccttc ggaacgggaa atgctggcat taccgctgta
     3841 cgttttgttg ccgttggcat cgtaatagtc gttaaaaata gcccccgtcc aggtatgatt
     3901 tttgttccaa taggtattca aacctaattc ggtgcgtgca accaaacctt tgacaatttc
     3961 ccagtttaaa gccgtattgg ccctaaggct gcgattgttt ctttcaggag tatagtcttt
     4021 gattctggct accggattga attcatcctg aagcaattcg tcgtacattc ccagggaact
     4081 gtttaccata ggatccaact ctcccatgac atgctgagtg acaatgggac ggaaaaaata
     4141 agaactcgat aaaacggaac cccgtgtgtt ggtcgtgctt tcatttccta cgctattggt
     4201 gtgcgtaaat ctggtatcga gcgataaggt gagttttttg tatagtttat gatccagctt
     4261 gatagaagca ttggttcttt tataccagga attcactttc agaccatcgt catcgatgtg
     4321 gttaaacgca actaaaaaat ttgttttgtc gttgccaccg gtgatgttca ggttgtggct
     4381 atgcgaaaaa ctttcgccgt aaacctgacg ctgatagttt tgggccacta cattcttgta
     4441 gtaatctata ccgggagtat tgccgtaggt ggcggcgtaa ggccctatgg cccacaacat
     4501 ttcccaggca tcccgat
//
LOCUS       DAOSGL010000186         4474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17535_length_4474_cov_3.397375, whole genome shotgun sequence.
ACCESSION   DAOSGL010000186 DAOSGL010000000
VERSION     DAOSGL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17535_length_4474_cov_3.397375"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKY68_06380"
     CDS             <1..449
                     /locus_tag="PKY68_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HOH24696.1"
                     /translation="VSVETVTLGTITMDGSYAYDSGASAYKWTLSTSPTTADFSATQS
                     SGNVFASGTLTTSAANVPGLTGMLMIPQAVNANYEIYVKYKINTEVFERTIKLSDFKK
                     SGTSLGTWDPGYKYTYNIVIGPTPILFDIIGVSDWGDGGVYTYTIE"
     gene            640..2043
                     /locus_tag="PKY68_06385"
     CDS             640..2043
                     /locus_tag="PKY68_06385"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HOH24697.1"
                     /translation="MLATIWPGVSVAAGPVDYEASSHNDTVEAPFFDTIRIKTTDTIY
                     FELKFRFDNYCLDMDYMENRIRMERFDSVLDSIGMENVLKFEIISLSSPEGTYEYNKR
                     LSERRSATMNRFMQDNYPHLIDIMETSPAGESWQMLRDYVAKDTCLSTQSIEKVLQLI
                     DADIDIAAKKWRMKTSLGKDSKLGDIYAYLYETYYPRIRFSGITIEHHIEIVNIIERP
                     QMEIVPTITIPYHPDTIVDFYLTDTVVEPCKVDTFIEPYKPDTVVSPTFRRFPKLAVK
                     TNMLYNATFTPKLGYAPILNVELEYYLAEDGRWSLLGEYEFPWWSKESEHQYLQILNL
                     QLEARYYLKKHSQFTRHYLSAYSGANLFDLCFDDKEGSGVQGEGVHAGLGYGYVLPLN
                     KKGNWKLELFLKGGFYIAYYDKYDAGEPFTGKYYYHWFGNISDFKPRSWRFRWLGPTG
                     AGVTISYDLFYRKKQNK"
     gene            2084..3169
                     /locus_tag="PKY68_06390"
     CDS             2084..3169
                     /locus_tag="PKY68_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HOH24698.1"
                     /translation="MKTYKSIMAVLCLALLTTAGCQSELLIDDQQHRQAVADTIDFVG
                     GFIDKPVNTKSTALLAEHLNTMGVWGWQITPEGDELKLFDNQIVSYNNATGDWTYSPK
                     KLWSDDSQYRFYAYAPHSDSTAGSTVSIDPVTGRFKIDDITLGGSNIMYNTPLRAASG
                     NFNGVTDIDWMIDRTGLAGVKSSFGRKVTFNMQHILVKFNVKVRINNILEADPSIVSV
                     TLDSLTIGTFIGHGSFEQKLNHTPDPGNADDNAVQEWTIDPSKPRYSINSARNVGIDA
                     TGFYVIESLLIPQEVDPGNTVKISYTTTSHDAHTEKYTGIFNLNNVFDRFVGGNNYTL
                     VITISPDIITFDAGSGSWETAFTNGVF"
     gene            3203..>4474
                     /locus_tag="PKY68_06395"
     CDS             3203..>4474
                     /locus_tag="PKY68_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HOH24699.1"
                     /translation="MKKYSIVFLVMAAMLTQSCRDNDLLYDFDGSPVSSQTIEFSNYV
                     SGITRASRTPGNSFVLNDEMQVYGFQTADGVTSQIFVDQLVKNIGSGKWSYTPKKYWS
                     NGSAYDFYAIFPYDAVNNSFDNTDRKFSVNDFTVADVTDDQVDLMIAQRIENRISQNT
                     VNFEFNHILSNVNFYIKAATTFDTEGIGSIKVLEFDVTGLYSKGSFAQSGWNGSNVFE
                     GTWTADNASVYNMPQVTNVVYTIGNPREPLVEDLLLLPQTINDNAEVYVRYMLNYTDG
                     TQTIFKRTVSLNKIMGAKKSTPEAKITLNKWDPNYRYNYTIALDPSKNENAGDTIPSD
                     PTDPDYPATPFIDYDGGVGNYQTPDAYLVKDANGDFWVDTDNDGIGDIKILWKDIDGD
                     GKLEGVADKNADGILDATDTYDGDGKDYNDNP"
BASE COUNT         1328 a         1023 c         1020 g         1103 t
ORIGIN      
        1 cggtatcggt tgaaacagtg acattaggta caatcacaat ggacggcagt tacgcctatg
       61 actccggtgc cagtgcctac aagtggactc tttccacatc tccgacaaca gcagacttca
      121 gtgccactca gagcagcggc aatgtttttg cttcggggac cttgactact tcagcggcta
      181 atgttcccgg acttaccggt atgcttatga ttcctcaggc agttaatgca aattatgaaa
      241 tctacgtcaa gtataagatc aatactgaag tttttgagag aaccataaaa ctgtcggatt
      301 ttaagaaaag cggaacctca ttgggaacct gggatccggg ctataagtac acatacaaca
      361 ttgtaatcgg tccgacaccc atactgtttg acataattgg tgtatccgat tggggcgacg
      421 gcggagtcta cacttataca attgaataat atcggataac tcttttcttt aaatagaccg
      481 gaatgggggt aacacccctt ccggtcacct tagaagagaa ataaaatatg gagactaagg
      541 ctgcccgaac gctaacatcg ggaagtaaca atgaagtaac aataaagaac ggacgatcgg
      601 atgagactta aaggaattat aatactgata catgcagcta tgttggcaac aatatggccg
      661 ggtgtttctg ttgctgccgg tcccgttgac tatgaagctt cgtctcacaa cgacacggtt
      721 gaggctccgt tttttgacac tatccgtata aaaacaaccg ataccatata ctttgagttg
      781 aaattccgtt tcgacaacta ctgcctcgat atggactata tggagaacag aatcagaatg
      841 gaacgcttcg acagtgttct ggattccata ggtatggaga atgttttaaa atttgagatc
      901 ataagccttt cgtcgcccga gggtacgtac gaatacaata aacggctgtc cgaaagacga
      961 tcggctacga tgaacaggtt tatgcaagat aactacccgc acctgataga tataatggag
     1021 accagtccgg ccggcgaatc atggcagatg ctgcgagatt atgtcgcaaa ggatacatgc
     1081 ctcagtaccc agtccataga aaaggtgctg caactaattg atgccgacat tgatatagcg
     1141 gcaaaaaaat ggcgcatgaa gacttctttg ggtaaagaca gcaagctggg tgacatttac
     1201 gcttatctct atgaaaccta ttacccgagg atacgttttt cgggcataac cattgagcat
     1261 cacattgaaa ttgtcaatat aatagaacgg ccacagatgg aaatcgtacc gacgataact
     1321 ataccttatc atcccgacac tattgttgac ttttatctga ccgatactgt agtggaacca
     1381 tgtaaggttg atacttttat cgaaccgtat aaacccgata ccgttgtttc gccgactttc
     1441 cgcaggttcc caaaattggc ggtaaaaacc aacatgttat acaacgccac gtttacgcct
     1501 aaattgggat acgctccgat tctcaatgtc gaactggagt attatcttgc cgaagacggc
     1561 cggtggtcgc tgcttggcga gtacgaattt ccgtggtggt cgaaagaaag cgagcaccag
     1621 tatttgcaga tacttaacct gcagcttgaa gcccgttact atctaaagaa acacagccaa
     1681 tttacaaggc actatctcag cgcctattcc ggagcgaacc tgtttgacct ctgctttgac
     1741 gataaggaag gctcgggagt acaaggtgag ggtgtccacg cgggtttggg ctacggctat
     1801 gttttgcctc ttaacaaaaa aggaaactgg aaacttgaat tgttccttaa aggcggtttt
     1861 tatatcgcgt attacgacaa gtacgatgca ggtgaaccat tcaccggaaa gtactactat
     1921 cactggtttg gcaacatcag tgatttcaag ccccgaagct ggcggttcag atggctcgga
     1981 ccgaccggag cgggagtgac aatcagctac gatctgtttt acaggaagaa gcagaataaa
     2041 tgatttgtat tataagaaga tattataaaa agaaattggt ttgatgaaga cttacaaaag
     2101 cataatggcg gttctctgtc tggcgttgtt aacgacagca gggtgtcagt ctgagctgct
     2161 tatcgatgat cagcagcata ggcaggctgt agccgatacc attgattttg ttggcggctt
     2221 catcgataag ccggtgaaca ctaaatctac cgccttgctt gctgagcatt tgaacaccat
     2281 gggtgtatgg ggatggcaaa tcacccccga aggcgacgag ctgaaattgt tcgacaatca
     2341 gatcgtgtct tacaacaatg caacgggcga ttggacttat tcaccaaaga aactctggtc
     2401 agacgacagt cagtacaggt tttatgctta cgcaccccat tccgacagca cggcgggctc
     2461 aacggtaagc atagacccgg taacgggccg tttcaaaatt gacgatataa cacttggagg
     2521 tagtaatatt atgtataaca cgcctttaag agcagcatcg ggcaatttca acggggtaac
     2581 cgatatcgac tggatgatcg accgcaccgg gctggccgga gtgaaaagtt cgtttggcag
     2641 aaaagtgaca ttcaacatgc aacatattct tgtcaaattt aatgtcaagg tgcgtattaa
     2701 caacatccta gaggccgatc cgagtattgt ttccgtaaca ctcgacagtt tgaccatagg
     2761 cacattcatc gggcacggca gctttgaaca gaagcttaac catacgcccg accccggcaa
     2821 cgcagacgac aatgcggttc aggagtggac tattgatcca tcaaagccgc gttacagcat
     2881 aaacagcgcc aggaacgtcg gaatcgacgc tacaggattc tacgtaatag agtcgctcct
     2941 tatcccacag gaagttgatc ccggcaatac ggtgaagata agctatacaa cgacttcaca
     3001 tgacgctcat accgaaaagt ataccggtat ttttaatctc aacaatgtgt ttgatcgctt
     3061 cgtaggcggc aacaattata cgctggttat caccatatcc cccgacatca tcactttcga
     3121 tgccggatcg ggttcttggg agacagcatt tacgaacgga gttttttaat tgaaaataat
     3181 gaacaaaata aaacataaaa acatgaaaaa gtattctatt gtattcttag tgatggcagc
     3241 aatgctcaca cagtcctgcc gggacaacga cttgctgtac gattttgacg gttcgcccgt
     3301 gagcagtcaa accatcgagt tcagcaatta tgtcagcggc atcacccgcg catcgagaac
     3361 tcccgggaat tcgttcgttt taaacgatga aatgcaggtt tacggctttc aaaccgccga
     3421 tggtgtaaca tctcagattt tcgtcgatca gctggtaaag aacatcggca gtggtaagtg
     3481 gtcatacact cctaaaaaat actggagtaa cggatcggca tatgacttct atgccatatt
     3541 cccctacgat gctgtcaaca acagctttga caacacagac cgtaagttca gtgtcaacga
     3601 cttcactgtt gcagacgtca ccgatgacca ggttgatttg atgattgcac agcgcattga
     3661 aaaccgaatc tcacaaaaca cggtaaactt tgagttcaac cacatcctct ccaacgttaa
     3721 cttctacata aaggctgcaa caactttcga caccgaagga atcggctcta tcaaggtgct
     3781 tgaatttgac gttacaggtc tttacagtaa aggctctttc gctcagagcg gctggaacgg
     3841 cagcaacgta ttcgaaggaa catggacggc cgataacgca tcggtctata acatgcctca
     3901 ggtcacaaac gttgtttata ccataggtaa tccccgtgaa ccacttgttg aagatttgct
     3961 tttgctcccg caaacaatca acgacaacgc tgaagtatat gtaagatata tgctgaatta
     4021 cacagacggc acacaaacta ttttcaagcg tacagtcagc ctcaataaga ttatgggtgc
     4081 taaaaagtcg actcccgaag ctaaaattac gcttaacaaa tgggatccca actatcgcta
     4141 taattatacc attgctcttg acccgtcaaa gaatgagaat gccggtgata ccataccttc
     4201 cgatcccacc gatcccgact atcccgctac tccgttcatt gactacgacg gtggtgtagg
     4261 caattaccaa actcccgacg catatctcgt taaggatgca aacggcgatt tctgggtcga
     4321 taccgacaac gacggcatcg gcgacattaa gatcctttgg aaagacattg acggagacgg
     4381 aaaactggaa ggtgtggccg acaagaacgc agacggcata ctggatgcaa ccgacactta
     4441 cgacggtgac ggtaaggact acaacgacaa cccc
//
LOCUS       DAOSGL010000187         4467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17573_length_4467_cov_4.232548, whole genome shotgun sequence.
ACCESSION   DAOSGL010000187 DAOSGL010000000
VERSION     DAOSGL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17573_length_4467_cov_4.232548"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /gene="rfbB"
                     /locus_tag="PKY68_06400"
     CDS             <1..1065
                     /gene="rfbB"
                     /locus_tag="PKY68_06400"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOH24700.1"
                     /translation="YVKYLLANYPDIRIIILDALTYAGNYGTIAGDVDGEGCIFVKGN
                     ICDQKTVRNLFETYPIEVVVNFAAESHVDRSIENPQLFLETNILGTQNLLDEAKRAWT
                     TGKDSQGYPAWKENVRFHQVSTDEVYGSLGDDGYFTEQTPLDPRSPYSASKTSADLFV
                     NAYVETFKMPATITRCSNNYGPYHFPEKLIPLIIKNILEGKPLPVYGKGINVRDWLYV
                     EDHCKAIEMVIRKGRPGEVYNVGGHNEKQNIEIVKMTIDSIREIMEKEPEYRSVLKTD
                     IERINYDLITYVADRLGHDMRYAIDPTKIGNELGWYPETKFEEGIQKTIRWYLKNQDW
                     VNDVTSGDYQQYYERMYGKR"
     gene            1062..1622
                     /gene="rfbC"
                     /locus_tag="PKY68_06405"
     CDS             1062..1622
                     /gene="rfbC"
                     /locus_tag="PKY68_06405"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOH24701.1"
                     /translation="MKFIQQAIPGVWVIEPKVYNDARGYFSEVFKQELFEQNVGNIWF
                     IQDNESKSTRGVLRGLHFQKGEYSQAKLVRVILGKVLDVAVDLRQNSPHFGRHVAVEL
                     SEENKRQFFIPRGFAHGFLVLSPEAVFTYKVDNVYAPQAEGSLSYADPELGIPWPITG
                     DELILSPKDAQAPLLKDAYTFPGLLP"
     gene            complement(1595..3580)
                     /locus_tag="PKY68_06410"
     CDS             complement(1595..3580)
                     /locus_tag="PKY68_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOH24702.1"
                     /translation="MKTYQKTDWLPVTKKEMQLRGWEQADVVLFSGDAYVDHPSFGAA
                     VAGRLLEACGMKVALVPQPDWRGDHRDFTKMGKPRYFFAISAGCMDSMVNRYTANKRL
                     RSDDAYTAGGLPGARPDYTTITYANILKKLYPEVPIVIGGIEASMRRLSHYDYWSDRL
                     MPGILVDSKADLLVYGMGEKPLKEIAGLLLSGAPFEALRELRQVVYLRQAGDTVGKEI
                     SSCIQLHSHEECLKSKRKQAENFRVIEEESNKMEASVLIQRVGEMEIVVNPPHPPLSS
                     EELDASFELPYTRLPHPKYKGKPIPAYEMIKFSVNLHRGCFGGCSFCAIAAHQGKFVV
                     SRSKESVLKEVSLLSQRSDFKGYLSDLGGPSANMYRMQGQNPEICRKCKRNSCLFPKI
                     CSNLNVDHRPLLDLYQAVDALPGIKKSVISSGIRYDLLLYSSQQALKSGKYDQTAIKE
                     AADKDRAAQEYTRQLIEKHVSGRLKVAPEHTENSVLNSMRKPPFSQFIQFREIFEQIE
                     NRRLCEQRKNQNFRTNEPHKNQNLPSTESLKGESKIKGRQELVPYFISSHPACSEEDM
                     AELAAKTKTLNFRLEQVQDFTPTPMTLATEMYYTGLNPYTLKPVYTAKTREEKLAQRQ
                     YFFWYRPEDKASIVKALRRLKRVDLIEKLYGKRPGKV"
     gene            complement(3584..>4467)
                     /locus_tag="PKY68_06415"
     CDS             complement(3584..>4467)
                     /locus_tag="PKY68_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOH24703.1"
                     /translation="LQDIVRDKRYWEKKRLEIKRLEKDLAQLTTDYREQMELIKQERK
                     QILGKAKTQAEQLLSRANAEIEGTIKSIRESQAEKEQTKLLRANLDKFKQQALKEEHE
                     AGHSLKPKASQTTARKQKILSKLESKTQKKAAVETEINFVPGDIVHLKGQNVPGKVLE
                     VKGGKITVAFGSIKSTVTPDKLGKSKAAKIKDLPDKSLGLKISEDVHRIGLNFKQEID
                     LRGLRGDEALQQVMYYIDEAVVVGVSSVRLLHGTGTGALRTLIRNYLGTVPEVKSFRD
                     EHVQLGGAGITVVEFKH"
BASE COUNT         1043 a         1022 c         1125 g         1277 t
ORIGIN      
        1 tacgtaaaat atttgttggc taattacccc gatatccgga taatcattct ggatgctctt
       61 acttatgccg ggaattacgg caccatcgcc ggggatgtgg acggggaagg ctgtattttt
      121 gtcaaaggca acatttgcga tcaaaaaacg gtcagaaatt tgtttgaaac ttatcccata
      181 gaggtggttg tgaattttgc tgccgaaagt cacgttgatc gcagcatcga aaatcctcaa
      241 ttatttttgg aaaccaatat tctgggtact caaaatttac tggatgaagc caagagagcc
      301 tggaccaccg gtaaggactc gcagggttat ccggcatgga aagagaatgt tcgtttccat
      361 caggtttcta cagacgaagt gtacggaagc ctgggtgatg acggctattt tacggagcaa
      421 acacccctgg atccccgcag tccctacagc gcttcgaaaa ccagtgccga tctgtttgtc
      481 aacgcttatg tggagacgtt taagatgcct gccaccatta cccgttgttc caacaactac
      541 ggcccttatc attttcccga aaaattaatt cctcttatca tcaaaaatat actcgaaggc
      601 aaacctctgc ctgtttacgg aaaagggatt aatgtccggg actggctcta cgtggaagac
      661 cactgcaaag ccatcgaaat ggtaatacgt aaaggtcgtc ccggcgaggt ctacaacgtg
      721 ggaggacata acgaaaaaca aaacatcgaa atcgtcaaaa tgaccataga ttccattcgc
      781 gaaatcatgg aaaaagaacc ggaataccgt tccgtactaa agaccgacat tgaacgtatc
      841 aattacgatt tgattaccta cgtggccgac cgcctggggc acgatatgcg ttatgccata
      901 gatcccacca aaataggcaa tgaattgggt tggtatcccg aaaccaaatt cgaagaaggc
      961 atacaaaaaa cgatacgctg gtatctgaaa aatcaggact gggtaaacga tgttacttcg
     1021 ggcgattatc agcagtatta cgaaagaatg tacggcaaac gatgaaattc atacagcagg
     1081 ccatacccgg tgtttgggta attgaaccca aagtctataa cgacgcccgg ggctattttt
     1141 cggaagtgtt caaacaggaa ctgtttgaac agaacgttgg caacatctgg tttatacagg
     1201 ataatgaatc caaatcaacg cgcggagtgc tgagaggctt gcactttcaa aaaggggaat
     1261 attcccaggc caaactggta cgggttattt taggcaaagt gcttgatgta gccgttgatt
     1321 tgaggcaaaa ttctccgcat tttggtcgac acgtagccgt ggagctttcg gaggagaaca
     1381 aaagacaatt ttttattccc cggggctttg cgcatggctt tctggtgtta agccccgaag
     1441 ccgtatttac ctacaaagtg gataatgtct atgctcctca ggccgagggc agtctttctt
     1501 atgccgatcc cgaattgggc ataccctggc ccattacggg agatgaactc atcttgtctc
     1561 ccaaggacgc acaggctcct ttacttaagg atgcttatac ttttcccggt cttttgccgt
     1621 aaagtttttc tataagatcg acgcgtttca gacgccggag tgctttcaca atagaagctt
     1681 tatcctccgg gcggtaccaa aagaaatatt gccgctgagc cagcttttct tctctggttt
     1741 ttgccgtata aacaggtttg agcgtgtaag gattcagtcc tgtgtagtac atttctgtgg
     1801 ccagagtcat gggcgtaggg gtaaagtctt gcacttgttc cagtctaaaa ttaagcgttt
     1861 tggttttggc ggccaattcg gccatatctt cttcgctaca ggcaggatgg gaggaaataa
     1921 aatagggtac aagttcctga cgtcctttga ttttactttc gcctttaaga gattcagttg
     1981 aagggaggtt ttggttttta tgaggttcgt ttgtgcgaaa gttttgattt tttcgctgtt
     2041 cgcacagacg acgattctcg atttgttcaa agatttccct gaattgtatg aactgtgaaa
     2101 acgggggttt gcgcatagag tttaatacgc tgttttcggt atgttccggt gctactttca
     2161 gccggccgga tacgtgcttt tcgatcagtt gacgagtgta ttcctgcgca gctctatctt
     2221 tgtctgcggc ctctttaatg gcggtttgat catatttgcc tgattttaaa gcttgttgtg
     2281 aagagtaaag caagaggtcg tagcggatgc cactgcttat tacacttttt ttgatgccgg
     2341 gcagggcatc tactgcttgg tataaatcga gcaagggccg gtggtctacg ttgaggttgg
     2401 aacagatttt cgggaaaagg caggaattac gtttgcattt tcgacaaatc tccgggtttt
     2461 gcccctgcat gcgatacatg ttggccgaag gtccgcccaa atcgctcaga tagcctttga
     2521 aatcgcttct ttggctgagc aggctcactt ctttcagtac cgattccttg ctgcgactca
     2581 ccacaaattt accctgatgg gcggcgatgg cgcagaagga gcaaccgccg aaacaacccc
     2641 gatgcaggtt gaccgagaat ttgatcattt cgtaggccgg aatgggctta cctttgtatt
     2701 tgggatgggg caggcgggta tagggtaact caaagctggc gtcgagctct tcgctgctaa
     2761 ggggtggatg aggcgggtta accacaattt ccatttcccc gacgcgctga atcaggacgg
     2821 aagcctccat tttgttggat tcttcctcta tgacgcgaaa attttcggcc tgtttacgtt
     2881 tgctcttcag gcattcttcg tgcgaatgca attgaatgca ggaggatatt tccttgccta
     2941 ctgtatcgcc cgcttgcctc aggtagacga cttgcctgag ctcgcgtaac gcttcgaagg
     3001 gagctcccga gagcaaaagt ccggctattt ctttcagggg tttttcgccc atgccgtaga
     3061 ccagcaggtc ggccttgctg tcgaccagaa tgcccggcat aaggcggtcc gaccaataat
     3121 cgtaatggct gagtctgcgc atggaggctt ctatgcctcc aatcactatg ggaacttcgg
     3181 gatagagttt tttcagaatg ttggcgtaag tgatggtagt gtagtctgga cgggctcccg
     3241 gcaaacctcc ggccgtatag gcatcgtcgg aacgcagacg tttgttggcg gtatagcggt
     3301 tgaccataga gtccatgcaa ccggccgaaa tggcaaaaaa gtaccgcggt ttccccattt
     3361 tggtaaagtc gcgatgatct cctctccagt cgggctgcgg tacgagggcc actttcattc
     3421 cgcaggcttc cagcagacgc cctgccacgg cggccccgaa agaggggtgg tctacataag
     3481 catcacccga aaacaagacg acatcggctt gttcccagcc cctgagttgc atctcttttt
     3541 tggtaacggg cagccagtcg gttttttggt aggttttcac agcctaatgc ttaaattcga
     3601 ctacggtgat gccggcacct cccaactgaa cgtgctcatc tctgaaactt ttgacttcgg
     3661 ggacggtacc caggtagttg cgtatcaatg ttctcagtgc cccggtaccg gtgccgtgca
     3721 aaaggcggac gctggaaacg cccacaacga cggcttcgtc aatgtaatac attacctgtt
     3781 gcagcgcttc gtctccccgc aagcctctga ggtcgatttc ctgtttaaag tttaaaccga
     3841 tgcgatgaac gtcctcactg attttcagtc ccaggctttt gtccggaagg tcttttattt
     3901 tggcagcctt gctttttccc aatttgtccg gggtgaccgt agatttgata ctgccaaatg
     3961 cgacagtgat tttcccgcct tttacttcga gcactttccc cggaacgttt tgacctttca
     4021 gatggactat atcgccggga acaaagttga tttccgtttc tacagcggct ttcttctgtg
     4081 ttttggattc cagtttgctc agtatttttt gtttgcgggc agttgtttgc gaggctttgg
     4141 gtttgaggga atgtcccgct tcgtgttctt cttttaaagc ctgttgtttg aatttatcga
     4201 gattggccct cagtagttta gtttgttcct tttcggcttg ggattcccgg atgcttttga
     4261 tggtgccttc aatttcggcg ttggcccggc taagcagttg ttcggcttga gttttggctt
     4321 taccgagtat ttgtttgcgc tcctgtttga tgagttccat ctgctcccgg taatcggtgg
     4381 tcaattgagc aaggtctttt tcgagacgtt tgatttcgag gcgcttcttt tcccagtaac
     4441 gtttgtcacg tactatatcc tgcaagt
//
LOCUS       DAOSGL010000188         4455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17638_length_4455_cov_4.517045, whole genome shotgun sequence.
ACCESSION   DAOSGL010000188 DAOSGL010000000
VERSION     DAOSGL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17638_length_4455_cov_4.517045"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..825
                     /gene="ubiE"
                     /locus_tag="PKY68_06420"
     CDS             115..825
                     /gene="ubiE"
                     /locus_tag="PKY68_06420"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOH24704.1"
                     /translation="MPKKKAVKHLFDRIAPYYDRLNHLLSLHVDKIWRRKAVKKVLVV
                     PHERVMDMACGTADFSLALAKAGVPSVLGLDISEQMLRIGRQKIQSQGMSHIIQLEQG
                     DSEAIAYPDKSFDVVTVAFGVRNYENLELGLQEMLRVLKPGGMLLVLELSLPQSRFLY
                     ALYSFYFTRILPWIGGLISGDKQAYAYLPASVLRFPKPKEFLQIMQSCGCVETRQQAL
                     SLGLARIFTGKKQITGKK"
     gene            complement(809..1705)
                     /gene="menA"
                     /locus_tag="PKY68_06425"
     CDS             complement(809..1705)
                     /gene="menA"
                     /locus_tag="PKY68_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOH24705.1"
                     /translation="MKSIKLYIKTFRPRTLPLSVSGIVLGSLLAASEGYFNVLIFILA
                     ILTTLSLQILSNVSNELGDAQKGTDTADRKGPAYVLLSGALTQKDFYRLIAIFSLLSV
                     VFGSLLVYVSLDYFFSKEGLIMLAFGGLAILASITYTLGKNPYGYKGWGDLFVFVFFG
                     LLSTLGSYFLQCGQLPLLLLLPASACGFLITGVLNLNNIRDMETDKINRITLPLVLGK
                     PKAKIYHHLLVYGAFVLILIYNLLKGTAWPQYLFLLLLPLAAFHLNRVRALNDEALDR
                     QLPVLVLLTILFSLIVGFTSFL"
     gene            complement(1752..2576)
                     /locus_tag="PKY68_06430"
     CDS             complement(1752..2576)
                     /locus_tag="PKY68_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH24706.1"
                     /translation="MTSSQTKPYAIGLALSGGGAKGFAHAGALKALEEFGIYPDIISG
                     TSAGAIVGSLYADGCTPHEIIELFNNTSFYKFASFARSKTGLFKIDNFRKAMNQKLKA
                     KTFEELNVPLYVNATDMFHGKNVFFSSGSLLDKVIASCSIPIIFEPVLIDGVHYSDGG
                     LLCNFPVEVLRKKCKYLIGVNVGLVDIEHEEQLNLLDIIQRTYFFIRSASVLKGREQC
                     DLLIEPPSIDKYGMFDAVKNEEIFQLGYQAALAILNEQKEKVKEMQAVSLSCRKTR"
     gene            complement(2709..>4455)
                     /locus_tag="PKY68_06435"
     CDS             complement(2709..>4455)
                     /locus_tag="PKY68_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOH24707.1"
                     /translation="RQGPQDTGQLASRFTGSGLEVQEALDGASDIIAEWVNENSRARH
                     QVRNHFRRSALIRSVVIKDKESEGQNYRDYFDYSEPLRSCSSHRFLAMIRGRNEGFLS
                     ISLKPDDEACLDSLERMFVRANNPAGALVAEAVKDAYKRLLKPSIENEFLSSSKEKAD
                     REAIRVFAANLKQLLLAPPLGQKRVLAIDPGYKSGCKVVCLDAQGKLLHNENIFPHLD
                     QRQKVLAAKKMAQLVESYQIEAIAIGNGTASRETEAFVKALRLDRAVQVYVVSENGAS
                     VYSASAIAREEFPQYDVTVRGAVSIGRRLCDPLAELVKIDPKAIGVGQYQHDVDQQML
                     KKALDQTVVHCVNAVGVNLNTASKHLLSYVSGLNSQSAARIVEYRDENGAFNLRSELL
                     KVPSVGPKSFEQAAGFLRIPGSVHPLDNTAVHPERYALVEKMAADLGCSTEDLIRNAD
                     LRKKIPLENYLDEHTGWPTLNDIMQELDKPGRDPRAKIEIFEFDPNIRKFEDLQEGMV
                     LPGIVTNITNFGCFVDIGIKENGLLHLSQMADRFVNQATDVVSLHQHIKVRILALDPE
                     RRRIQLSLKNNPDAS"
BASE COUNT         1084 a         1037 c         1138 g         1196 t
ORIGIN      
        1 tctgatcttc aagatcgatt atctgatgaa tgtttccaat ccccaggatg attttgtgcg
       61 aggccccaga gtctatttcg gttttgtgtt ctatcacatc aaacgctaag cttcatgccc
      121 aaaaagaaag ccgttaagca tttattcgac cggattgccc cgtattacga ccgcttgaat
      181 catttgttgt cgctccatgt ggataagatt tggagacgta aggctgtcaa aaaagttctt
      241 gtggttcctc acgaacgggt aatggatatg gcttgcggaa cggccgattt cagcctcgct
      301 ctggccaagg ccggtgttcc ctcggtgtta gggctcgata tttcggaaca aatgctgcgt
      361 atcggccggc aaaagattca gtctcagggc atgagtcata ttattcaatt ggaacagggc
      421 gacagcgaag ccattgctta tcccgacaag agttttgatg tcgtaaccgt ggctttcggt
      481 gtacgcaatt acgaaaactt agagctgggc ttgcaagaga tgctgcgggt actcaaaccg
      541 ggaggaatgc ttttggtttt ggaactttct ctgcctcaga gtcgattttt gtatgctctt
      601 tattcttttt atttcacccg cattttacct tggataggcg ggctgatatc gggcgataag
      661 caagcctatg cttatttgcc ggcttccgtt ctgcgttttc ccaaacccaa ggaattccta
      721 caaattatgc aatcctgcgg ctgtgtcgaa acccggcagc aagctctgag cctgggattg
      781 gcccgtattt ttacaggaaa gaagcagatt acaggaaaga agtaaatccc actatcagac
      841 taaacaggat ggtcagcagc accagaaccg gcagttgccg atcgagcgcc tcatcgttga
      901 gcgcccgcac acgattcagg tgaaatgccg ccagaggaag caacagtaaa aaaagatatt
      961 ggggccaggc cgtgccttta agcagattgt aaatcaggat cagcacaaaa gccccatata
     1021 ccagcagatg gtgatagatt ttggctttag gtttgcccaa cacaaggggc agcgtgatgc
     1081 ggttgatctt gtcggtctcc atgtcccgga tattgttcag gttgagtact ccggtgataa
     1141 gaaaaccaca ggccgaggcg ggaagtaaca gcaaaagagg cagttgtccg cactgcaaaa
     1201 aataagatcc cagggtactc agcaagccga aaaacacgaa gacaaacaaa tcgccccagc
     1261 ctttgtagcc gtagggattc ttgcctagag tgtaagtgat ggaagcaaga atggccaggc
     1321 ccccaaaagc cagcatgatc aggccttctt tactgaagaa ataatccagt gagacgtaca
     1381 ccagcaaact gccgaagacc acgctaagca ggctaaagat agcaatcagc cggtagaaat
     1441 ccttttgggt gagagctccc gaaagcagca cataagccgg gcctttgcga tcggcggtat
     1501 cggtgccttt ttgggcgtcg cccaattcgt tggacacgtt cgacagaatt tgcagagaca
     1561 gcgtggttag gatggccaga ataaagatca ggacgttgaa atacccttcg gaagcagcca
     1621 gcaaactgcc caggacaatg ccggaaaccg ataggggcag ggttctgggg cggaaagttt
     1681 taatatagag tttaatagat ttcatgtttc attcgtatga aacgctcttg acggtggcag
     1741 tttaggcgaa cttaccgtgt tttccggcag gaaaggctga cagcctgcat ttcttttact
     1801 ttttcttttt gttcatttaa gatggccagg gctgcctgat aacccaattg aaaaatttct
     1861 tcgtttttga cggcgtcgaa cataccgtac ttatcgatcg aaggcggttc aatcaagagg
     1921 tcgcattgtt ctcttccttt taaaacgctg gcactacgaa tgaaaaaata agtgcgttga
     1981 ataatgtcca aaaggttgag ttgctcttcg tgttctatat ccaccaggcc tacgttgaca
     2041 ccaatcaggt atttgcattt cttgcgcagc acttcaaccg ggaagttgca taggagaccc
     2101 ccgtcggaat aatgaacccc atcgatcaat accggttcga aaataatggg aatactgcag
     2161 gaagctatga ccttatccaa caaactaccg gagctgaaga atacgttttt gccgtgaaac
     2221 atatccgttg cgttcacgta cagagggacg ttcagttctt cgaaagtttt agctttaagt
     2281 ttttgattca tagcctttcg gaaattgtct attttaaaca gaccggtttt ggatcgggca
     2341 aagctggcaa atttataaaa agaggtgttg ttgaaaagtt caatgatttc gtggggagtg
     2401 cagccgtcgg catacaaaga tccgacgatg gccccggcgc tggtgcccga aatgatgtcg
     2461 ggataaatac caaattcttc cagggcctta agcgcaccgg catgggcaaa gcctttagcc
     2521 cctccgccgc tcagggccag accgatagca tatggtttcg tttgtgagga tgtcatatac
     2581 ttataatttg acgatgcaaa agcccggtaa aattaaggca ttttgtctga tgcggcaatt
     2641 ttgttttttg ctcagtaagc ttcgtttttc tcagtaagct tcgtttttct cagtaagctt
     2701 cgtttttttc aggaagcatc gggattgttt tttaaactta attgaatacg cctgcgttcc
     2761 ggatcgaggg ctaaaattct caccttaatg tgttgatgca gactcaccac atcagttgct
     2821 tgattaacaa agcgatcggc catttgagaa agatggagta aaccgttttc cttgatgcct
     2881 atatccacaa aacaaccaaa gttggtgatg ttggttacta tgccgggtaa aaccattcct
     2941 tcctgcagat cctcgaattt tctgatattg ggatcgaatt caaagatttc gatttttgcc
     3001 cggggatcgc gtcccggttt atcgagttct tgcataatat cgtttagagt gggccaaccg
     3061 gtgtgttcgt ccaggtagtt ttccagcgga atttttttgc gtaggtctgc gttacgaatc
     3121 aggtcttcgg ttgaacagcc caaatcggct gccatttttt cgaccagggc gtagcgttcc
     3181 gggtgtacgg ccgtattatc gaggggatga acagagccgg gtatgcgcag gaaacctgcc
     3241 gcctgttcga agcttttggg gccaacggag ggaaccttaa gcagttcgct gcgcagattg
     3301 aaagctccgt tttcgtctct gtattcgacg atgcgggcag ccgactggct attcaggccc
     3361 gatacgtaac tcaacaggtg tttgctggcc gtgttcaggt ttacgcctac cgcattgacg
     3421 caatgtacca ccgtctgatc cagggctttt ttcagcattt gttgatcgac gtcgtgctgg
     3481 tattggccaa cccctatggc tttgggatcg attttgacca gctcggccaa gggatcgcat
     3541 aaacgccgtc ctatggatac tgcaccgcgc acggtgacat cgtactgggg aaattcttct
     3601 ctggcgatgg ccgaagccga atacaccgaa gctccgtttt cgcttaccac atacacctga
     3661 actgcccggt cgagccgcaa agctttgaca aaagcctcgg tttctctcga tgctgtgcca
     3721 ttgccgatgg cgatggcttc gatttgatag ctttctacca gttgggccat ttttttggct
     3781 gccagcactt tttgccgctg atccaagtga gggaagatgt tttcgttgtg caggagtttg
     3841 ccttgtgcat cgaggcaaac caccttacaa ccgcttttgt agccggggtc tatggccagg
     3901 actcgctttt gtcccaaggg aggagccagc agcaattgtt tcaggttggc ggcaaaaacc
     3961 cgaatggctt cccggtcggc tttttctttg ctcgaactta ggaattcgtt ctcgatagaa
     4021 ggttttagaa ggcgtttgta agcatctttc acggcttctg caaccagtgc gccggcgggg
     4081 ttgtttgcgc gcacaaacat acgttccaga ctgtcgagac aggcttcgtc gtcgggtttg
     4141 aggctgatgc tgaggaaacc ttcattcctg ccccgaatca tggccagaaa gcgatgagaa
     4201 gagcaggaac gcaggggttc gctgtaatcg aaataatccc ggtagttttg tccttcactt
     4261 tctttgtctt tgatcactac agagcggatt agagcgctgc gtctgaagtg attcctgacc
     4321 tgatggcggg cccggctgtt ttcgttcacc cactcggcta tgatgtcgga agcaccgtca
     4381 agagcttcct gcacttcgag gcccgaaccg gtaaaacggg aggccagttg cccggtgtcc
     4441 tgcggccctt gtctc
//
LOCUS       DAOSGL010000189         4452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17661_length_4452_cov_4.315442, whole genome shotgun sequence.
ACCESSION   DAOSGL010000189 DAOSGL010000000
VERSION     DAOSGL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17661_length_4452_cov_4.315442"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1766)
                     /locus_tag="PKY68_06440"
     CDS             complement(<1..1766)
                     /locus_tag="PKY68_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH24708.1"
                     /translation="MPKQLFIDPSEMRKAGEVTFKPIPVNQYNKTAKQELDEGNFSKQ
                     DLLDIYHDMSLIREFETMLHLVKTTGAYQDIPYNNPGPAHLSVGQEAAAVGMAYTLGV
                     DDFIFGSHRSHGEILAKGMSAIRKLDEDSLKKIMQEFLGGATLKVAGMNFEGNTRQLA
                     RRFLVYGALAEIFARVTGFNRGLGGSMHAFFTPFGIYPNNAIVGGSGDIAVGAALYKK
                     INRKPGLVVANLGDASMACGPVWEGLVFAAMDQFKTLWDDDMKGGLPFILNIMNNQYG
                     MGGQTQGETMGYDIAARIGAGINPEQMHAERVDGYNPLAVIDAYRRKRQVINDKQGPV
                     LLDVLTYRYSGHSPSDASSYRSKEELEAWEAQDCIMGYAKQLLEGNICKQADLDRIQE
                     DIRKTILEVYKLAIDLDISPRMDLDQNQELLGEAMFSNQSVDSMMPGVKPEVNHPMHE
                     NPRVQQIAKKERFAFDAEGKPHSKMRQYQLRDGIFEAIIDRFYKDASLVAYGEENRDW
                     GGAFAVYRGLTEALPYHRLFNSPISEAAIVGTAIGYAMCGGRVIPEIMYCDFLGRAGD
                     EVFNQMPKWQAMSGNILKMPIVL"
     gene            complement(1793..3202)
                     /gene="lpdA"
                     /locus_tag="PKY68_06445"
     CDS             complement(1793..3202)
                     /gene="lpdA"
                     /locus_tag="PKY68_06445"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOH24709.1"
                     /translation="MYDLAIIGGGPAGYVAAERAGSQGLSVLLFEKASLGGVCLNEGC
                     IPTKTLLYSAKTYENALQGDKYGITATGVEFDFGKMMARKNKVVRKLVAGVEAQMRAG
                     KVTVVKAAAYIKGRSDKGVELIAAPQERQEAGEIYFAAHLMICTGSEAMVPPIPGLAE
                     AGEILLTNREILQLKEQPAELVVIGGGVIGMEFASFFNSLGSKVTVVEMLPEILGGLD
                     LEISAMLRNIYAKKGIDFHLQAKVVKVQGNEVFFEKDGQTQSVKGDKILLSVGRRAVT
                     QGYGLENLGVELHRGGIKVDELMRTNIPGVFAAGDVTGFSLLAHTASREGEVVVNNIL
                     AARQAAQSGSKVRYDRMRYNAIPGVVYTNPEVASAGLSEEAARAANIEFKAIKLPMAY
                     AGRFVAENEGGNGLCKIIVDSRNGRVLGVHMLGNPCSEIIYGACMAIEQEITLEELKQ
                     VVFPHPTVSEIIKEVAYNA"
     gene            complement(3286..>4452)
                     /locus_tag="PKY68_06450"
     CDS             complement(3286..>4452)
                     /locus_tag="PKY68_06450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOH24710.1"
                     /translation="AEMDAGAQGSGQTAPSASAASTTEIPAAGVTAPAPAAQASAPAS
                     PDQPGAVASQAGAASTSSVTASGGTVFISPRARKLAEKEALDFRQLSGSGPGGRIIES
                     DMKAALQNRPKLSPLAKKTLQEQAAVVPEQGSGLAGTVLASDLKAPVNAVYGIDYQDK
                     KLSNMRKLIAKSMYASLQNSAQLTHHLGADARRIMELRKQVKEAMDQGKLNVNITLND
                     MVCLAVIKALKKFPNANSHFLGDSMRLFNKVHLGLAVDTERGLMVPALRNADDLSISG
                     LASQLKELAAACRKGAIDPDLLSPEAASFTVSNLGNYGVEMFTPVINLPQSAILGVNT
                     IVPRPKDLGGGVYAFVPYIGLSLTYDHRALDGGEATRFLKQIAVEIENLEFDIV"
BASE COUNT          920 a         1136 c         1259 g         1137 t
ORIGIN      
        1 agcacgatgg gcatttttag aatgttgccg ctcatagcct gccatttggg catttggttg
       61 aagacttcgt cgccggcccg gcccaaaaag tcgcaataca tgatttcggg gatcacccgc
      121 ccgccgcaca tggcgtaacc gatggccgtg cccacgatgg cggcttcgga aatgggtgaa
      181 ttgaacagac ggtgataagg caaagcttcg gtaagtccgc gatacacggc aaaggcgccg
      241 ccccagtcgc ggttttcttc tccgtaggcc accagcgaag cgtctttata gaaacgatcg
      301 atgatggctt cgaagatacc gtcgcgcaac tggtattgcc tcattttact gtggggttta
      361 ccctcggcat cgaaggcgaa acgttctttt ttggcgattt gctgtaccct gggattttcg
      421 tgcatggggt gatttacttc gggcttcact ccgggcatca tgctgtcgac cgattggttg
      481 gaaaacatag cctcgcccag caattcctga ttttggtcca aatccatgcg gggagaaatg
      541 tcaaggtcga tggccaactt ataaacttcc aaaatcgtct tgcggatgtc ttcctgtatc
      601 cggtcgagat cggcttgctt gcagatgttg ccttcgagca attgcttggc gtagcccata
      661 atgcaatcct gagcttccca ggcttcgagt tcttctttgc tgcgatagga cgaagcgtcc
      721 gagggggaat ggccgctgta gcgataggta agcacatcga gaaggacagg cccctgtttg
      781 tcgttgatca cctgacgttt acggcgatag gcatcgatca ctgccagggg attgtagccg
      841 tctactcttt cggcatgcat ttgttcgggg ttgataccgg ctccgatacg ggctgcaatg
      901 tcgtaaccca tggtttcgcc ctgggtttgt ccgcccatac cgtactgatt gttcatgatg
      961 ttgaggataa aaggcagtcc gcctttcata tcgtcgtccc aaagagtttt gaattgatcc
     1021 atggcggcaa aaaccagacc ttcccaaacg ggaccgcaag ccatggaggc gtcgcccagg
     1081 ttggcgacca ccaagccggg tttacggttg atttttttat agagggctgc tcctacggca
     1141 atatcgccgc taccgcctac gatggcgttg ttgggataaa tgccgaaggg ggtgaagaaa
     1201 gcgtgcatac taccgcccag gccccgattg aaaccggtta cgcgggcaaa gatttcggcc
     1261 agagcgccgt agaccaaaaa gcgacgggcc agttgccggg tgttgccttc gaagttcatg
     1321 ccggccacct taagagtggc tccgccaagg aattcctgca tgattttttt aaggctgtct
     1381 tcgtcgagct tacgaatagc cgacatgccc ttggccagga tttcgccgtg gctgcggtgt
     1441 gagccgaaaa taaagtcgtc tacgcccaga gtataggcca taccgacggc ggcagcttcc
     1501 tgtcccaccg agaggtgagc aggaccgggg ttattgtagg gtatgtcttg gtatgctccg
     1561 gtggttttga ccagatgcag catggtttcg aattccctga tcagggacat gtcgtggtaa
     1621 atgtcgagca aatcctgttt gctgaaattg ccctcgtcca gttcctgttt ggctgtttta
     1681 ttgtactggt ttacgggaat gggcttgaat gtaacttccc cggctttacg catttccgag
     1741 gggtcgatga ataattgttt aggcataatg aaattatttt atgtgttctg ttttatgcgt
     1801 tgtatgctac ttctttgatg atttcgctga cagtgggatg aggaaagacc acttgtttga
     1861 gttcttcgag ggtaatttcc tgctcaatgg ccatgcaagc tccataaatg atttcgctgc
     1921 agggattgcc cagcatgtgc acgcccagta cccggccgtt ccggctgtcg acaatgattt
     1981 tgcagagacc gttgcctcct tcgttttcgg ccacaaaacg cccggcatag gccatgggga
     2041 gtttgatggc cttaaactcg atattggcag cccgggcggc ttcctcgctg aggccggcgc
     2101 tggctacttc ggggttggtg taaaccacac ccggaatggc gttgtagcgc atacggtcgt
     2161 aacgaacctt gcttcccgat tgtgcggcct gacgggcggc caggatattg ttgaccacta
     2221 cttctccttc gcgcgaggcg gtatgagcca acaggctgaa accggtgaca tcgcccgcag
     2281 caaaaacgcc cggaatattg gtgcgcatga gttcgtccac cttgataccg ccgcgatgaa
     2341 gttctacacc caggttttcg aggccgtatc cctgagtaac ggcgcgacgg cctacactga
     2401 gcaaaatctt atcgcccttg accgattggg tctgaccgtc tttttcgaag aaaacttcgt
     2461 tgccttgtac tttgactact ttggcctgca aatggaagtc gatgcctttt ttggcgtaaa
     2521 tgttgcgcaa catggccgag atctccaggt cgagaccgcc caaaatctcg ggcagcattt
     2581 ctacgacagt gactttactg cccaggctgt tgaagaaact ggcaaattcc atacctatga
     2641 ctcctcctcc aataactacc aattcggcgg gttgctcttt tagttgcagg atttcgcgat
     2701 tggtgagcag gatttcgcct gcctcggcca ggccggggat gggcggcacc atggcttcgg
     2761 agccggtaca aatcatcaga tgggcggcaa aatagatttc gcctgcctcc tgcctttcct
     2821 gcggggcagc gatcaattct acccccttat cggagcggcc tttgatatag gctgcggctt
     2881 tgactacagt caccttgccg gccctcattt gagcttcgac gccggccacc aatttgcgaa
     2941 cgactttgtt tttgcgggcc atcattttgc caaagtcgaa ttcgactccg gtggctgtaa
     3001 taccatactt atcaccctga agtgcatttt cgtaggtttt ggcactgtag agcaaagttt
     3061 tggtgggaat gcagccttcg ttcaggcaaa caccgcccag actggctttt tcgaaaagca
     3121 gcaccgataa gccctgactt ccggctcttt cggcggctac gtatccggcg gggcctccgc
     3181 caatgattgc taaatcgtac atagtggttg gaatttattt atttcgattg tttttaattt
     3241 tcttcttggg atttatggga tttaattacc gacaagcccg agcctctaga caatatcaaa
     3301 ttcgagattt tcgatttcga cggcgatctg cttcagaaag cgggtagctt cgcctccgtc
     3361 cagtgcccga tggtcgtagg tgaggctcag gccgatgtag ggcacgaagg catacacgcc
     3421 gccacccagg tctttgggac ggggtactat ggtatttacg cccaggatgg ccgattgcgg
     3481 caggttgatg acgggagtaa acatttccac tccgtaattg cccaggttgg atacggtaaa
     3541 gctggcggct tcggggctaa gcaggtcggg gtcgatggct cccttgcggc aggcggcggc
     3601 caattccttg agttgcgagg ccaggccgct gatgctcaga tcgtcggcgt tgcgcagggc
     3661 gggtaccatc aatccgcgtt cggtatcgac ggcaagaccc agatgcacct tgttgaacaa
     3721 gcgcatactg tcgcctaaga aatgggagtt ggcattaggg aattttttaa gggccttgat
     3781 aacggccaaa cacaccatat cgttgagggt aatgttgacg ttgagtttgc cctggtccat
     3841 ggcttccttg acctgcttgc gtaattccat aatacggcgg gcgtcggccc ccaggtggtg
     3901 ggtaagttga gcggaattct gcaaagaggc atacatggat ttggcaataa gtttacgcat
     3961 attgctcagt ttcttgtcct gatagtcgat gccgtaaaca gcattgaccg gagccttgag
     4021 gtcggaagcc agtacggtgc cggccaggcc gctgccttgt tcgggcacaa cggctgcctg
     4081 ttcttgcagc gtttttttgg ccagagggct cagcttggga cggttttgca aagctgcctt
     4141 catatcgctt tcgatgatac ggccgccggg gccgctgccg ctgagctgac ggaaatcgag
     4201 ggcttctttt tcggccagtt tacgggcacg gggtgaaata aaaacggtac caccggaggc
     4261 ggtaacggaa cttgttgagg ctgcacctgc ttgcgaagcg actgcgccgg gctgatccgg
     4321 gcttgctggg gcggaagcct gcgctgccgg agcaggggct gttacacctg cagccggtat
     4381 ttcagtcgta gaagcggccg aagcagaagg tgctgtttgg cccgaaccct gagcgccagc
     4441 atccatttca gc
//
LOCUS       DAOSGL010000190         4436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17760_length_4436_cov_3.181237, whole genome shotgun sequence.
ACCESSION   DAOSGL010000190 DAOSGL010000000
VERSION     DAOSGL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17760_length_4436_cov_3.181237"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="PKY68_06455"
     CDS             <1..1361
                     /locus_tag="PKY68_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24711.1"
                     /translation="KEQAEFISGPAAHHFYLQPAGEKLSFTCLFLDRNPLLDSSELPA
                     CPESEQVAQASSDYWNQFWLNGGAVDFSACTDPRAPELERRVVLSQYLMAIQCAGVYP
                     PQESGLTYNTWYGKFHLEMHWWHGVHFVLWGRPELLERSLDWYRTAEANAANIAQRQG
                     FKGVRWMKMTDPSAIEAPSKVGSFLIWQQPHIIYMAELLFRHQKDSSVLADYSDLVFK
                     TAEFMADFADYDGMGDRYILQGAIPAQETLRASETVNSPFELSYWHYALSVAQQWRLR
                     LGLPKNMEWEIVLDKLSPLAHKDGLYLAAETALDTYIDTRYTSDHMAVLGALGMMPKS
                     RLVREDIMTNTFNWIWDNWNWGKTWGWDYPMTAMCAARIGLPEKAVDALLMDRRTNTY
                     LVNGHNYQDGRLRVYLPGNGGLLTAVAMMCAGWDGSEGKNPGFPNNGQWKVKWEGLDV
                     MP"
     gene            1377..2720
                     /locus_tag="PKY68_06460"
     CDS             1377..2720
                     /locus_tag="PKY68_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOH24712.1"
                     /translation="MKWILRVFPALFLIFSAGLTEVNAQNKLAFPGAEGFGRFATGGR
                     GGSIYRVTNLNDSGEGSLRDAVSQPNRIVVFDVSGVIRLQSALVFSKNLTVLGQTAPG
                     DGIQVYGDQVSFSGADNIIVRYLRIRMGIDGTDGKDAAGVANGKNMIFDHMSVLWGKD
                     ENFSVSWGNRGTEPTNITIQNSIIGQGLQPHSCGGLIQTGGGVTLFRNLFIENNTRNP
                     KVKGLNQYVNNVVYNWGDGCYIMGGSEGPSWAHIENNYFINGPWEGATPVFTRGNVNF
                     TFYGAGNYHDANRNGLLDGSLMTADQYSASIPVSDWATWDNSTARPQPHPEILSMTTA
                     QEALAWIIDSVGPCLPVRDEVDQYLIDELVSYGTKGTTKGITTEKTLPHQGTGTLRRG
                     SKPPDTDQDGIPDAWETANGLNPNDPSDAAAIAANGYSNIENYSFSITACIKSPL"
     gene            2794..4215
                     /gene="cls"
                     /locus_tag="PKY68_06465"
     CDS             2794..4215
                     /gene="cls"
                     /locus_tag="PKY68_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOH24713.1"
                     /translation="MNVVFQQIVYALVALTVLSVVLVIISENRNPVKSVAWILLLIFL
                     PLLGLIAYFLIGQDFTKKYAYKRKKHRGKDQAFLIKSDQVLDEQDYPRAFTAPVKLLK
                     NIQNALLIGGNDVKYFTLAADKYRQMLADMEEAKRFIHLEYYALENGLWGDALKDLLI
                     RKSARGVEVRIIYDDWGCWRTKKSYFKELIDNGVQVRVFSKVRILPLTARLNYRDHRK
                     LIIIDGLIGYIGGMNISDRYVNGFNWGCWADTHCRIAGPGVVQLQSVFLSNWQRQSGE
                     VLWEDEYFPLPASCGDNALQVVASGPGSLDPEILHVMIQAFYSAEKYIYVQTPYFIPP
                     EPLAGALQAAAIRGLDVRLMISKRSDLPLVQMASRSYIREMLERGIKVYFYTRGFLHS
                     KMMVVDGELSIMGSANADMRSFEDNLEVQAFIYGHSAALRAQEIFLEYQQDCEKINLE
                     RWKKRSRLKRFVESFMRLFAPLL"
     gene            complement(4229..>4436)
                     /locus_tag="PKY68_06470"
     CDS             complement(4229..>4436)
                     /locus_tag="PKY68_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH24714.1"
                     /translation="DANGLRLNSWDEVLDIQVVNINGLTVADLKSKATEWEKDIKELN
                     LAKGIYLIKLQSGKSSRIVKHYQP"
BASE COUNT         1031 a         1080 c         1183 g         1142 t
ORIGIN      
        1 ggaaggagca ggccgaattt atctccggcc ccgcggcgca ccatttttac ctgcaacccg
       61 ccggcgaaaa gctgagtttt acctgccttt tcctcgatcg taatcctttg ctcgattctt
      121 ccgaattacc ggcctgcccc gaatcggagc aggtggctca ggcatcctcg gattattgga
      181 accaattttg gctcaacgga ggcgccgtcg acttttcggc ttgtaccgat ccccgtgcgc
      241 cggagctgga acgccgggtg gtgctctcac aatatttgat ggccattcaa tgtgccggtg
      301 tttatccgcc tcaggaaagt gggctcacgt ataacacctg gtacggtaag ttccatttgg
      361 aaatgcattg gtggcatggg gtgcattttg ttctctgggg acgtcccgaa ttgttggaac
      421 gcagtctcga ctggtaccgt acagcagaag caaatgccgc taatattgct caacgtcagg
      481 gcttcaaggg agtgcgctgg atgaaaatga ccgatccttc ggctatagaa gctccttcga
      541 aggtgggctc ctttttgatt tggcaacaac cgcatatcat ttatatggcc gaattgttgt
      601 tccgtcacca aaaggacagt tccgtcctgg ccgactacag cgacctggtg tttaaaacgg
      661 ccgaatttat ggccgatttt gccgattacg acggaatggg agaccgctac atcctgcaag
      721 gggccattcc ggcgcaggaa accctgaggg cttcggaaac cgtcaattcc ccctttgaac
      781 tctcttactg gcattatgcg ttgagtgtgg ctcagcaatg gcgtttgcgt ctgggtttgc
      841 ctaaaaatat ggagtgggag atcgtgctgg acaagctttc gcctctggcg cacaaagacg
      901 gactgtatct ggctgccgaa acggccttgg atacctacat tgacacccgt tacacttccg
      961 accacatggc ggtgttggga gctctgggga tgatgcccaa atcgagactg gtacgcgaag
     1021 atattatgac caacacgttc aactggattt gggacaactg gaactggggc aaaacctggg
     1081 gctgggatta ccccatgacg gccatgtgtg ccgcccgtat cggcctgccc gaaaaggctg
     1141 tggatgcctt gcttatggat aggcgcacca atacctacct ggtcaacggt cacaattatc
     1201 aggacggccg cttgcgggtg tatctacccg gaaacggagg cttgcttacg gccgtagcca
     1261 tgatgtgtgc cggctgggac ggtagcgagg gtaaaaatcc cggtttcccc aacaacggcc
     1321 aatggaaggt gaaatgggaa ggcctggatg tgatgccttg atcagaaaaa gtaattatga
     1381 agtggatatt gcgcgttttt ccggctttgt ttttgatttt ttctgccggt ttaacagagg
     1441 taaatgccca aaataaactg gcttttcccg gcgccgaagg ttttggccgt tttgccaccg
     1501 gaggcagggg aggcagtatt taccgtgtaa ccaacctcaa cgactcgggc gaaggttccc
     1561 tgcgtgatgc cgtcagccag cccaatcgaa tcgttgtctt cgatgtttcg ggcgtgattc
     1621 gtttgcaatc ggctttggtt ttttccaaga accttactgt gttgggtcaa actgctccgg
     1681 gcgacggtat acaggtgtat ggcgatcagg tgtcgttttc gggcgctgat aacatcatcg
     1741 tgcgttatct gcgtattcgt atggggatag acggcaccga cggcaaagac gccgcgggcg
     1801 ttgccaacgg caaaaacatg atcttcgatc atatgtcggt gctttggggc aaagacgaga
     1861 acttttcggt ctcttggggt aataggggta cggaaccaac gaatattacc attcaaaatt
     1921 ccattattgg ccagggtttg cagccgcatt cctgtggtgg tctgattcag accggaggag
     1981 gtgtcactct gtttcgtaac ctcttcatcg aaaataatac ccgtaatccc aaagtaaagg
     2041 gactgaatca atacgtcaac aacgtggtct acaactgggg cgacggctgc tacatcatgg
     2101 gcggttccga agggccctcc tgggcgcaca tcgaaaataa ttacttcata aacgggccct
     2161 gggaaggagc aacgcctgtg tttacaaggg gtaatgtcaa ttttactttt tacggggccg
     2221 gcaattacca cgatgcaaat aggaacgggc tgctcgacgg tagtctgatg acggccgatc
     2281 aatattctgc ttcgataccg gtgtccgatt gggctacctg ggacaattct actgccagac
     2341 ctcaaccgca tccggaaatt ttgagtatga cgactgctca ggaagccctg gcctggatta
     2401 tcgacagtgt agggccctgt ctgccggtgc gcgacgaggt agatcaatac ctgatcgatg
     2461 aattggtttc ttatggaacc aaaggcacca ccaagggcat tactactgaa aagaccttgc
     2521 cgcaccaagg tacaggcacc ctgcgcagag gttctaaacc ccctgatacc gaccaagacg
     2581 gaattcccga tgcctgggaa acggccaacg gactcaatcc gaacgatcct tcggatgctg
     2641 ctgccattgc cgccaacggc tacagcaaca tcgaaaatta ttctttcagt ataaccgcct
     2701 gcatcaaatc gcctttgtaa tcgagactaa acacttgttc tcgaagcgat atgacagaat
     2761 ttaggcgatt tatatattta aaaaggtttt gttgtgaacg tagtctttca gcaaattgta
     2821 tatgctctgg tggcccttac ggtgctgagc gtcgtgctgg tgattatttc ggaaaaccgc
     2881 aatccggtca aaagtgtagc ctggatactg ttgctcattt ttttgccctt gctgggcctg
     2941 atcgcttatt tccttatagg acaagatttt accaagaagt atgcctataa aagaaagaaa
     3001 cacagaggta aagatcaggc ttttctgatc aagagcgacc aggtgctgga tgaacaggac
     3061 tatccccggg cttttaccgc tccggtaaaa ttgctcaaaa acatccagaa cgcccttctg
     3121 ataggcggca acgacgtgaa gtactttact cttgccgccg acaaataccg gcaaatgctg
     3181 gccgatatgg aagaagccaa gcgttttatt cacctggaat attacgcact tgagaacggc
     3241 ctctggggcg atgctcttaa ggatttactc atacgtaaat cggccagagg ggtggaggtc
     3301 agaatcattt acgacgactg gggttgttgg agaaccaaaa aaagctactt caaagagctg
     3361 atagataatg gagtacaagt acgtgttttt tcaaaagttc gtatactgcc cttgacggcc
     3421 cgcctcaatt accgtgatca ccgcaaattg atcatcatcg acggccttat cggatatatt
     3481 ggcggaatga atatcagcga ccgctatgtg aatggtttca actggggctg ttgggctgat
     3541 acccattgcc ggattgccgg tcccggggtg gtgcagttgc aatcggtctt tttgtccaac
     3601 tggcagcgtc agtcgggcga agtcttgtgg gaggacgagt attttccctt gccggcttct
     3661 tgcggcgaca acgctttgca ggtggtggcc agcgggccgg gcagcctcga tcccgaaatc
     3721 ttgcacgtta tgattcaggc tttttacagc gccgaaaaat acatttatgt tcagactccc
     3781 tactttattc ccccggaacc gctggcaggc gccctgcagg cggcagccat ccgcggtctg
     3841 gacgtacgtc tgatgatttc caaacgttcc gatctacctc tggtgcaaat ggcttcgcgc
     3901 tcttatatcc gtgagatgct cgagcgtgga ataaaggtct atttctacac ccggggtttt
     3961 ttgcactcca aaatgatggt ggtcgacggg gaattgagca ttatgggctc ggccaacgcc
     4021 gatatgcgga gtttcgaaga caatctcgaa gtgcaggcct ttatctacgg ccactctgcc
     4081 gctctcaggg ctcaggagat atttctcgaa tatcagcagg attgtgaaaa aattaacctg
     4141 gaacgatgga aaaaacgttc caggttaaaa cggtttgtgg agtcttttat gcgtctgttt
     4201 gctcccttgc tttaaaactt aagccggctt agggttggta gtgcttgaca atccggctgc
     4261 ttttgccgct ttggagtttg atcaggtaaa tgcctttggc caggttcaac tccttgatgt
     4321 ctttttccca ttccgtggct ttacttttca ggtcggcgac ggtaagtccg ttgatgttga
     4381 ccacctgtat gtcgagcact tcgtcccagc tattgagccg cagtccgttg gcatcg
//
LOCUS       DAOSGL010000191         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_17954_length_4398_cov_3.670965, whole genome shotgun sequence.
ACCESSION   DAOSGL010000191 DAOSGL010000000
VERSION     DAOSGL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17954_length_4398_cov_3.670965"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="PKY68_06475"
     CDS             complement(<1..1706)
                     /locus_tag="PKY68_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOH24715.1"
                     /translation="MSVLRFNLVQEAFSHKAVPADIPAKKVSDYYGIHVFHRETMSKY
                     LPGNIYNQLLKDIDSGKALNITMANHLAKAMKIWAMELGVTHYTHWFHPLTDGTAEKH
                     DALIQLEESGRVIEAFNGSLLIQQEPDASSFPSGGIRNTFEARGYSAWDPSSPAFVVD
                     DTLCIPTIFISYTGEALDYKTPLIKAISAVGKAAAETCRFFYPEVKKVNSNLGWEQEF
                     FLVDEALWSARPDLMMCGRTLMGHEAAKNQQLDDHYFGTIPKRVVEFMKELEYEAYKL
                     GIPLKTRHNEVAPNQFELAPIYEETNLAVDHNLLLMDIMNKVAPKHHFKVLLHEKPFD
                     GVNGSGKHCNWSLSTDAGLNVFSPGKTAEGKLMFTVFLVNTLMAVHKHNALLKASVAS
                     AGNARRLGLNEAPPAIVSVFLGSQLSDILDKMAESTAEEAIQIDPRGVLRLDVGQIPE
                     LMLDNTDRNRTSPFAFTGNRFEFRALGSSANCASAMIVLNTAVAGQLMDFNAALNAKM
                     AAGESKEKAVFSILRQYIRESKPIRFEGNGYSDEWKAEAAKRGLDCENSVPLQYDAYL
                     RPS"
     gene            1793..3877
                     /locus_tag="PKY68_06480"
     CDS             1793..3877
                     /locus_tag="PKY68_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH24716.1"
                     /translation="MNKVPFRHQILRFRRFIHKSYAVFNSLNKSVSIGKLSAAVCDLA
                     LKKSKNAGLLALLFTGIVSFSLQASDPEDPDWTVDLDSAGITATPPLSFPAHQRAVYS
                     VGHSQIAHFPAQHFNELLDALPGLDLRQRGQQGVQADLSMRGGSFDQVLVLLNGINIT
                     DPQTGHYNLDLPVDLAAIERIDVLQGSSLSLSGSPAFSGAVNIVTGYSGKNKLSVDLG
                     AGQYGWDRRSLQIQQKLKQWYLNASWSQVRSSGYIKNTDFDKHNLFVQLQAPSLPSGN
                     WNIQLGLQQKEYGANKFYSLLYPDQFEATQTLLSSLTWNKLLDRYKASASAYFRLHSD
                     RFELFRQTTAAPAWYRSHNHHLSDVSGLDLNLSRYGPAGKSMIGFNIRNEHILSTVLG
                     QPLEKSRRSAFLPDSVVYTNSAGRLGLTLFAEQQFNGPNFNSSLGLSVNKYAGYGARI
                     CAEAAFAYLFSDRSRLFVNAQRSLRYPSFTDLYYKSPVQVSNPNLKPETAYSAETGFS
                     YNEQRLQAGISLFGRLGSNIIDWVKQAGEEVWHSMNHSRVNAAGAEALISWKPQKHLR
                     LLESSYTFTILNKESGELMSKYALDYLKHKFNTRLEHSIYKDFAASWHFSAQQRMGTY
                     NDPEGNLQHYRPFCLLDGRLFWSRKELTLYLEAGNLFNTSYYDYGGILQPGRWLRAGI
                     RIQLDRDLLSNH"
     gene            complement(3958..>4398)
                     /locus_tag="PKY68_06485"
     CDS             complement(3958..>4398)
                     /locus_tag="PKY68_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOH24717.1"
                     /translation="LPLVIISLILVSFIGTFLTFYFSGINFGTGGFASLVLLCGIVVN
                     SGIYVVNEYKDTRLRNQERGLGVSRLKMYLKAYNHKIIPVFLTVLSTVLGLVPFFIDS
                     ETDDFWFSFAVGTTGGLLFSIVALVFVMPIFMPLIKRNRPRRKG"
BASE COUNT         1162 a         1162 c         1039 g         1035 t
ORIGIN      
        1 gaaggacgca aataagcatc gtattgcagg ggcacgctgt tttcgcaatc cagtcctctt
       61 ttggcagctt ccgccttcca ttcgtcgctg tagccgttcc cttcgaagcg tatgggtttt
      121 gattctcgga tgtattgacg caaaatggaa aaaacggctt tttctttgga ttcgccggcc
      181 gccatttttg cgttcaaagc ggcgttgaaa tccattaatt ggccggccac ggcggtattg
      241 agcacaatca tggccgaagc gcaattggcc gatgaaccca gggctctgaa ttcaaaacga
      301 ttgcctgtaa aagcaaaggg cgaagtccgg ttacggtcgg tattgtcgag catcaattcg
      361 ggaatctgcc ccacatcgag gcgcagcaca ccgcgcggat ctatctgaat ggcctcctct
      421 gccgtggatt ccgccatttt atcgagtatg tcgctgagct gactacccaa aaacaccgat
      481 acgatggccg gaggggcctc gttcagcccc aggcggcggg cattgccggc cgaagccacc
      541 gaagccttga gcagggcgtt gtgtttgtgt acggccatga gggtgttcac caaaaagacc
      601 gtaaacatga gttttccttc ggcggttttg cccggcgaaa agacgttaag gccggcatcg
      661 gtgctcaaag accaattgca atgcttgccc gatccgttca ctccgtcaaa gggcttttcg
      721 tgcaacaaca ctttgaaatg atgtttggga gccaccttat tcattatatc catcaaaagc
      781 aggttgtgat ccacggccag gttggtctcc tcgtaaatgg gcgccagctc aaactgattg
      841 ggagccacct cgttgtgtct ggttttaaga ggaataccca atttataagc ctcgtactcg
      901 agttctttca taaattccac cactcttttg ggaatggtgc cgaaataatg gtcgtcgagc
      961 tgctgatttt tggcggcctc gtgccccatc agggttcgtc cgcacatcat caaatcgggg
     1021 cgcgcactcc agagagcttc gtccaccaaa aagaattcct gttcccaacc caggttagag
     1081 ttcacttttt tgacttcggg ataaaagaaa cggcaagttt cggcggccgc tttaccgaca
     1141 gcggaaatgg ctttgatcag aggcgtttta tagtcgagcg cttcgccggt gtaggaaata
     1201 aaaatagtgg gtatgcaaag ggtgtcgtcc acaacaaagg ccggcgaaga gggatcccag
     1261 gcgctgtagc ccctcgcttc gaaagtattg cgtatgccgc cgctcggaaa actcgaggca
     1321 tcgggttcct gttgaataag caggctgccg ttgaaggctt cgatcacccg gccgctttct
     1381 tccaactgta tcaaggcatc gtgtttctcg gccgttccgt cggtaagcgg atgaaaccaa
     1441 tgggtataat gcgtcacacc caattccatg gcccagattt tcatagcctt ggccagatga
     1501 ttggccatgg ttatattcaa ggctttgcct gaatcgatgt ctttgagcaa ttgattgtag
     1561 atgttgccgg gcaggtattt actcatggtt tcgcgatgaa acacatggat gccgtaataa
     1621 tcggaaacct tttttgccgg aatatcggca ggaacggcct tatgcgaaaa agcctcctga
     1681 acgaggttga aacgtaacac tgacataaga aagggcttaa ctaatcagca acaaaggtaa
     1741 gacaaaaaat tttttatcta cctttgtccc cgttttttcg agcatacaaa aaatgaacaa
     1801 ggttcccttc aggcatcaaa tcttgcgctt cagacgattt atccacaaat cctatgcggt
     1861 attcaattct ttgaacaaat ctgtttccat cggaaaactg agcgcagcgg tttgtgatct
     1921 tgccctgaaa aaaagcaaga acgccggcct gttggccttg ctgttcacag gtatcgtttc
     1981 ttttagcctg caagcctccg atccggaaga ccccgactgg acggtcgatc tggacagtgc
     2041 cggaatcacg gcaactcctc ctttgagctt tcccgcccat caacgagccg tttattccgt
     2101 aggccactcg cagatagctc attttcccgc acaacatttc aacgaattac tcgacgccct
     2161 tcccggcctc gacctgcgac agcgcggtca acagggtgta caggccgatt tgtccatgcg
     2221 cggaggcagt tttgaccagg tattggtgct gctcaacggc atcaatatca ccgatcctca
     2281 aaccggccat tacaacctgg atcttcccgt cgatctggcc gccatcgaac gcatcgatgt
     2341 gttgcagggc agctcgctga gcctctcggg cagtccggct tttagcggag ccgtaaacat
     2401 cgtcaccggc tattcgggca aaaacaagct gagtgtcgac ttaggggcgg gacaatacgg
     2461 atgggaccgc cgcagcctgc aaatacagca gaaacttaaa cagtggtatc taaacgcttc
     2521 ctggtcgcaa gtgcgatcat cgggctatat caaaaacacc gattttgaca aacacaatct
     2581 gtttgttcaa ctgcaagccc cctctctgcc ttcgggcaac tggaacattc aactgggcct
     2641 ccagcaaaaa gaatacggag ccaacaaatt ctattccctg ctctaccccg atcaattcga
     2701 agccacacaa acgctgctgt cttcgctcac atggaacaag ctcctggaca ggtacaaagc
     2761 aagcgcttcg gcttatttca ggttgcacag cgaccgtttt gaactgtttc gtcaaacaac
     2821 tgcggccccc gcctggtaca gatcacacaa tcatcatttg agcgatgtaa gcggtctgga
     2881 tttgaacctg agccgttacg gccctgcggg aaaatcgatg atcggtttca atatacgcaa
     2941 cgaacacatt ctgagcaccg ttttggggca acctttggaa aaaagccgca gatcggcctt
     3001 tctgcccgat tccgttgttt ataccaacag cgccggacgt cttggcctaa ccctttttgc
     3061 cgaacaacaa ttcaacggcc ccaatttcaa ctcttcctta gggttatcgg tcaacaagta
     3121 tgcgggatac ggcgcccgga tctgtgcaga agccgccttt gcctatttat tttcggatcg
     3181 cagccgtctt tttgtcaatg cacaacgcag cctgaggtat ccgagtttta ccgatctcta
     3241 ttacaaaagt ccggtacaag tcagcaatcc caacctcaaa cccgaaaccg cctacagtgc
     3301 cgaaacgggt ttcagttaca acgaacaacg attacaggcc ggaataagcc tttttggccg
     3361 cctgggcagc aacatcatcg actgggtgaa acaagccggc gaagaagttt ggcacagcat
     3421 gaaccacagc cgtgtaaatg ctgccggcgc cgaagctctg atcagctgga aaccgcaaaa
     3481 acaccttcgt ttattagaaa gttcctacac ctttaccata ctgaacaaag agtccggaga
     3541 gctgatgtcg aaatacgccc tcgattacct gaaacataaa ttcaacaccc gactggaaca
     3601 ctctatctac aaagactttg ccgcaagctg gcacttcagc gcccaacaac gtatgggcac
     3661 ctacaacgat ccggaaggaa atctgcagca ctaccgcccc ttctgcctgc tggacggccg
     3721 cttattttgg agcagaaaag aactgaccct ctacctggaa gccggcaacc tgttcaacac
     3781 aagctattac gattacggag gcatccttca gcccggacga tggttgaggg cgggcatacg
     3841 gattcaactg gaccgggatt tgttatcaaa tcattaagga gattttcggg caaagcctac
     3901 taaacagaga caaaaagcct actaaacaga gataaaaagc ctaccaaaca gagataatca
     3961 gcctttgcgt cgggggcgat ttctttttat aagcggcata aaaatgggca taacaaatac
     4021 cagggccact atagagaaaa gcaaaccgcc ggtggtgccc acagcgaaag aaaaccaaaa
     4081 atcgtctgtt tcgctgtcaa taaagaaagg caccaagccc aggacggtag aaagtacggt
     4141 cagaaaaaca ggaatgatct tgtgattata tgctttcaga tacattttga gcctgcttac
     4201 ccccaagcct ctctcctggt ttctcagtct ggtgtctttg tattcgttca ctacataaat
     4261 acccgaattg accactatcc cacacaacaa caccagcgaa gcaaaaccgc cggtgccgaa
     4321 atttataccc gaaaaataga aggtcaggaa agtacctata aacgatacca gaatcaacga
     4381 aatgattaca agaggcaa
//
LOCUS       DAOSGL010000192         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_18010_length_4388_cov_4.759751, whole genome shotgun sequence.
ACCESSION   DAOSGL010000192 DAOSGL010000000
VERSION     DAOSGL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18010_length_4388_cov_4.759751"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="aroC"
                     /locus_tag="PKY68_06490"
     CDS             <1..724
                     /gene="aroC"
                     /locus_tag="PKY68_06490"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOH24718.1"
                     /translation="GGGRSSARETIARVVAGAFAKMLLNTMNISIEAFTSQIGPIALE
                     QQDSTLFSSELAAENPLHCPDREKAEAMMALIARVKEEQDSIGGVVSCVVRGLPVGWG
                     EPVFGKLHARLASAILSIPACKGFEYGSGFDVSQKGSQQNDAFYFDGERINTRSNHSG
                     GIQGGISNGQPVYFRAAFKPIASIRIPQETVDKEGAAVKIQIQGRHDPCALPRVLPVV
                     EAMAALVLADACLMAKSKERIV"
     gene            761..2830
                     /locus_tag="PKY68_06495"
     CDS             761..2830
                     /locus_tag="PKY68_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOH24719.1"
                     /translation="MSIILSCFGCTNRQAAFKEFDYKVDRFADMEIIRYQVPGFEDLS
                     LRQKKLVYYLSEAALWGRDIIYDQNYRHNLLIRHCLEAIYQYADVSRQGEDWQAFEVY
                     LKRLWVANGIHHHYSTDKFEPGFSQDFFVTALRAVPAEKLPSMESGRLTQEQNPLQTQ
                     DSLNTTQYADELAARLLPLIFDARLDAKRVNQAQGEDLIVSSACNYYRDLSQKEAEAF
                     YAVLNNPENPCPVSYGLNSRLVKEEGQLREEVWKIGGLYSPALEKITYWLELALEVAE
                     NPRQKEIIQSLIDYYHSGDLETFDQFSILWVQDLESKVDFINGFIESYGDPLGIKGSW
                     EAIVNFKNEEASKRTQIISDNAQWFEDHAPIADEFRKKEVKGVSAKVITNAIMGGDAF
                     PTTAIGINLPNADWIRRDYGSKSVTIENITEAYFQSSQGSGFNKEFMWSEAEVKASAK
                     YGFITGNLHTDLHECLGHGSGQLLPGVDPNALRSYGSVIEETRADLFALYFLADPKLV
                     ELGLLPNEEAYKAEYYEYVMNGLMTQLSRIEKGKQIEESHMRNRALIARWLLDQGSKD
                     KVMKLKKRHNKTYLVIHDYEALRQLTGELLAEVQRIKSEGDYAAAEALVENYAVQVDP
                     LLHQEILDRYAKLNLSPYKGFVNPLYKPVYNDKDEIIDIIVLHNENYVEQHLRYSKDY
                     AALPVEN"
     gene            2856..>4388
                     /locus_tag="PKY68_06500"
     CDS             2856..>4388
                     /locus_tag="PKY68_06500"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOH24720.1"
                     /translation="MTLFLVTSAGLRAETKASSEDEVKTCMRKATAFMMDKVSINGGF
                     VSSYLSDFSRRWGELEAYPSMIWIDGPTPAMANVLLDAYLATEDEYYYRQACRVADAL
                     IAAQLPCGGWNYTFDFAGEESLKQWYATVGKNAWGVSEYNYYYGNATFDDQNSYRPAN
                     FFLRIYLLKKDPRHKEVMERAIDFFLKSQYPNGGWPQRYPLRYDFPAKDGSPDYSSFI
                     TLNDGVMEKNIDFMLRCYYLLDDERCLSAARKGMDCLLSLQQAPPTAGWALQYNLDLQ
                     PAKARDYEPASVCVSTTSGTVMLMMEYYRLTGEKKFMQGIPAALRFLDEVAFCDSMLQ
                     LIERSEEKVGRSSENGKLCPRMVVPGSSFTPLFLHRRGSHIVNGDYYIDQNPLNTIAH
                     YSSFCTINTDVLRSRYEDLLNLPAEEISRNSPLLHPELREPLLQQFLYNKRPEGMRRF
                     LYIDQTRPVQEVLASLNEEGYWPTPLVLTTNPYIGEGSADDPVPELYEETMKHPFNTQ
                     FYR"
BASE COUNT         1180 a         1151 c         1059 g          998 t
ORIGIN      
        1 cggaggagga cgttcttcgg cccgcgaaac catagccagg gtggtcgccg gagcctttgc
       61 caaaatgctg ctgaatacta tgaacattag catagaagct tttacaagcc aaatcggtcc
      121 catcgccctg gaacaacagg atagcacgct gttttcttcg gagctggccg ccgaaaatcc
      181 gctgcattgc cccgatcgcg aaaaagccga agctatgatg gccttaattg cccgggtaaa
      241 ggaagaacaa gacagtatag gcggagtggt cagctgtgtt gtcaggggac ttcccgtcgg
      301 ctggggagaa cccgttttcg gcaaactcca cgcccgcctg gcctcggcca tcctgtccat
      361 tccggcctgc aaaggttttg agtacggttc cggtttcgat gtcagccaaa aaggctccca
      421 gcaaaacgat gccttctact ttgacggtga acgcatcaat acccggagta atcattccgg
      481 aggcatacag ggaggcattt ccaacgggca gccggtttat ttcagggccg ccttcaagcc
      541 catcgccagc attcgtatac ctcaggaaac agtcgacaag gaaggtgctg ccgtgaaaat
      601 acaaattcag gggcgacacg atccttgtgc actgcccagg gttttgccgg tagtggaagc
      661 catggcagcc ctggtactgg ccgatgcctg cctgatggcc aaatcgaaag aacgcatagt
      721 ttaaacaaat tcacgctata tgaaaaaagt cttttttctt atgagtatta ttttgtcgtg
      781 tttcggctgc accaacagac aagctgcctt caaagaattc gattacaaag tggatcgctt
      841 tgccgatatg gaaattatcc gctatcaggt gcccggtttc gaagacctaa gtctgaggca
      901 aaaaaaactg gtttattatc tttccgaagc agccctttgg gggagggata tcatttacga
      961 tcagaattac cgccacaatc tcctgatcag acactgcctg gaggccattt accaatacgc
     1021 cgatgtcagc cgccagggcg aagactggca ggctttcgaa gtttacctta aacgcctctg
     1081 ggttgccaac ggcatacatc atcactattc caccgataag tttgagcccg gcttttcgca
     1141 agactttttt gtaacagccc ttagggcagt tcccgccgaa aaactgccct ccatggaatc
     1201 cgggcgacta acacaggagc aaaacccgtt gcagacgcag gactcattaa ataccacgca
     1261 gtatgccgat gaactggctg cccgtttgct gcctctgatt ttcgatgccc ggctcgacgc
     1321 caaaagagta aaccaggctc agggcgaaga tctgatagtc agttcggcct gcaattatta
     1381 ccgcgacctt agtcaaaaag aggccgaagc tttttatgcc gtgctcaaca atcccgaaaa
     1441 cccctgcccg gtttcctacg gcttaaacag ccgcctggtc aaagaggagg gccaactgcg
     1501 cgaagaggtt tggaagatcg gcgggctgta ttcaccggcc ttggagaaaa taacctattg
     1561 gctggaactg gccttggaag tagctgaaaa tccacgtcaa aaagaaatca ttcaaagctt
     1621 gatcgattat taccacagcg gagatctgga aacttttgat caattcagca ttctttgggt
     1681 gcaggatctg gaatcgaagg ttgatttcat aaacggtttc atcgaaagct acggcgaccc
     1741 tctgggcatc aagggcagtt gggaagccat cgtaaacttc aaaaacgaag aggcctccaa
     1801 acgcacccaa atcatcagcg ataatgccca atggttcgaa gaccacgccc ctattgccga
     1861 tgaattcaga aaaaaagaag tcaaaggagt ttcggccaaa gtaattacca atgccattat
     1921 ggggggcgac gcctttccga ctaccgccat aggcatcaat ttacccaatg ccgactggat
     1981 acgccgcgat tacggttcca aatcggtcac catcgaaaac atcaccgaag cgtattttca
     2041 atcgtcgcaa ggcagcggtt ttaacaaaga gtttatgtgg tccgaggccg aagtaaaagc
     2101 ctcggccaag tacggcttca ttaccggcaa cctgcatacc gacctgcatg aatgcctggg
     2161 ccacggctca ggccagttat tgccgggtgt ggatcctaac gcgctcaggt cttatggctc
     2221 agtgatagaa gaaacccggg ccgatctttt tgccttgtac tttctggccg atcccaaact
     2281 ggtggaattg ggcctgcttc ccaatgagga agcctataaa gcagaatatt acgaatacgt
     2341 tatgaacggc ttgatgaccc aactgagcag aatagagaag ggcaagcaaa tcgaagaatc
     2401 gcatatgcgc aacagggccc tgatagcccg ttggttactc gatcagggca gcaaagataa
     2461 agtgatgaaa ttaaaaaagc gtcacaacaa aacctacctg gtcatacacg attacgaagc
     2521 cttacgtcaa ctgacggggg aattgctggc cgaagtgcaa cgcatcaagt ccgaaggcga
     2581 ttacgcggct gccgaagccc tggtcgaaaa ctatgccgta caggtagacc ctctgttaca
     2641 ccaggaaatt ctggatcgct acgccaaact gaacctgtcg ccttacaaag gattcgtaaa
     2701 tccactctat aaaccggttt acaacgacaa agacgaaatt atcgacatca ttgttcttca
     2761 caacgaaaac tacgtggaac aacacctgcg ttattctaaa gattatgctg cattacctgt
     2821 tgaaaactaa ttttagatac aagcttatct tgtgtatgac acttttctta gtgacgagtg
     2881 ccggcctccg ggccgaaaca aaagcaagtt ccgaagacga agtgaaaacc tgcatgcgca
     2941 aggccaccgc ttttatgatg gacaaagtga gcatcaatgg cggtttcgta agcagttacc
     3001 tgtccgattt ctcccgccgg tggggagagc tcgaagccta tcccagcatg atctggatag
     3061 acggccccac cccggccatg gccaatgtcc tgttggacgc ttaccttgcc accgaagacg
     3121 aatactatta ccggcaagcc tgccgggtgg ccgatgcttt aatagcagct caattgcctt
     3181 gcgggggctg gaattacacc ttcgattttg ccggtgagga atcgctcaaa caatggtacg
     3241 ccactgtcgg caaaaacgcc tggggagtga gtgaatacaa ttattactac ggcaacgcta
     3301 ctttcgacga ccagaacagc tacagaccgg ccaatttctt tttgcgtatt tatttgctaa
     3361 agaaagatcc caggcacaaa gaagtcatgg aacgagctat tgattttttt ctcaaaagcc
     3421 aatatcccaa cggcggctgg ccccaacgct atcccctcag gtacgatttt ccggctaaag
     3481 acggcagtcc cgactactcg agttttataa ccctcaacga tggagtgatg gaaaaaaaca
     3541 tcgattttat gttgcgctgc tattacctgc tcgacgatga gcgctgtctc tctgcagccc
     3601 gaaagggcat ggattgcctg ctatcgctgc agcaggcacc tcctaccgcc ggctgggctc
     3661 tgcaatacaa tctggactta caaccggcca aagcccgcga ttacgaaccg gcttcggttt
     3721 gcgtttcaac cacctccggc acggtgatgc tgatgatgga gtactaccgc ctgaccggag
     3781 agaaaaaatt catgcaaggc attcctgccg cccttcgttt tttggacgaa gttgcattct
     3841 gtgacagcat gttacaattg atcgaacgca gcgaggaaaa agttggacgc agcagcgaaa
     3901 acggcaagct ttgtccccgc atggtggtcc ccggcagcag ctttacgcct ctgtttttgc
     3961 accgcagagg ctcgcatatc gtaaacggcg attactacat cgatcaaaac cccctcaaca
     4021 ccattgctca ttactcttcg ttctgtacta taaataccga cgttttgcgc agcagatacg
     4081 aagacctgct gaacctgccc gccgaagaga taagccgcaa ttctcctctc ttgcatccgg
     4141 aactccgaga gcctttgctt caacaattcc tgtacaacaa gaggccggaa ggaatgcgca
     4201 gatttctgta tatagaccag accaggcctg ttcaggaggt tcttgcctcg ttaaacgaag
     4261 aaggttattg gcccacccct ctggtgttga cgaccaatcc ttacataggc gaaggcagtg
     4321 ccgatgaccc tgtacccgag ctgtacgaag aaaccatgaa acatcccttc aacacgcagt
     4381 tttatcgt
//
LOCUS       DAOSGL010000193         4386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_18032_length_4386_cov_4.104364, whole genome shotgun sequence.
ACCESSION   DAOSGL010000193 DAOSGL010000000
VERSION     DAOSGL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18032_length_4386_cov_4.104364"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..908
                     /locus_tag="PKY68_06505"
     CDS             48..908
                     /locus_tag="PKY68_06505"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOH24721.1"
                     /translation="MLIKRFVLSLMLLNLSGLLLAQTDVSLFWRKDSLQQTEIFSAEG
                     DLYRLVGHHGPAIENKFMALRLYFNHSGSIDVYSKKIHRLELAQALWYPSEKMQQEQG
                     FGCDEYKVGSTVGLGGINLWDGRQVVKLTATQGRTARVSKNEKGASMEMLAKGIAYKD
                     KMVDVLIRVSVNNTDRQATVEAVCTSGHKVQFLTGVNRHPGNSVFNRDGRIGVWGRHP
                     ADVSKAPVLIGGGMKYRPSDFAIKRVTDDAIQLISKPAKKLKTIIVSAGEREEELNTE
                     QAFFDYVKSF"
     gene            complement(1030..1284)
                     /locus_tag="PKY68_06510"
     CDS             complement(1030..1284)
                     /locus_tag="PKY68_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOH24722.1"
                     /translation="MKKDLHPTNYRPVVFKDMSNGDTFISRSTIQAKETIEIDGETLP
                     LVKLEISSTSHPFYTGKSKLVDTAGRVDKFLNRYGNRNKK"
     gene            1486..2481
                     /locus_tag="PKY68_06515"
     CDS             1486..2481
                     /locus_tag="PKY68_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOH24723.1"
                     /translation="MISYKELGLVNSREMFKKAIAGQYAIAAFNFNNMEQLQAIIQAA
                     VETRSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGLEHPQIVLHLDHGDTFELC
                     KSCIDMGFSSVMIDGSHHPYEDNVALTRKVVDYAHKYDVTVEGELGVLAGVEDEVSSA
                     VSHYTRPDEVVDFVTRTGVDSLAISIGTSHGANKFTPEQCTRDANGVLIPPPLRFDIL
                     EEIEKRIPGFPIVLHGSSSVPQDLVAIINKYGGAMKDSIGIPEEQLRRAAKSAVCKIN
                     IDSDGRIAMTAAVRQVLATKPAEFDPRKYLGPARDSLKELYKHKLINVLGSANKL"
     gene            complement(2567..3574)
                     /locus_tag="PKY68_06520"
     CDS             complement(2567..3574)
                     /locus_tag="PKY68_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH24724.1"
                     /translation="MQYTPSADRYTNSVGYNYCGKSGLQLPRISLGLWHNFGDVNDQD
                     EAKKMLFTAFDNGITHFDLANNYGTPFGSAETNFGKMLKNDFKSYRDELIISTKAGHL
                     MWDGPYGDWGSRKYMIASLNQSLKRMGLDYVDIFYSHRYDPNTPLEETMGALSDMVKQ
                     GKALYVGISKYPPDKAREAIRLLSENGTPCLIYQGRYSLFNPEVEQSIFPLVEEKNIG
                     FIGFSPLAQGMLSDKYLEGIPPNSRAGQEFGFLKKEQITPEKIEKIKQLNDFARKRQQ
                     TLAQMALAWCLRDKRVTSVIIGASSVKQLSENIQTLDNLHFTEEELRLIGSSVCRIQK
                     K"
     gene            complement(3610..>4386)
                     /locus_tag="PKY68_06525"
     CDS             complement(3610..>4386)
                     /locus_tag="PKY68_06525"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOH24725.1"
                     /translation="QVPELNLQFTSEGILRAVNMDLSLTESCSGKASKQETTISCPEN
                     ANDNSASSAASIHTQDMQLSGSSIRMAELAAKKVFELRDTRLSLLQGELGTMPQDGAS
                     LEKMLTELNRMETHYTELFTGSRRQSVRTEVLEYTPEKIPSYEILFRFSSQKGIVDKE
                     DLSGYPVHFLVHNPGTDAISQQKDSISGKKGETSQQTCALYYYHPNRIDISLLKGQTV
                     LFKDNFVIAQFGDLMQLPASEQLMVELDPETGAIVRIKNR"
BASE COUNT         1155 a          928 c         1024 g         1279 t
ORIGIN      
        1 tctttaattt tttaattagt tttgcacagt aacaaaaaaa caaaaatatg ctgattaaac
       61 gatttgtatt aagcttaatg cttctgaact tgtcggggct gttattggct cagaccgatg
      121 tgagcctgtt ttggagaaaa gattccctgc aacaaacaga aattttttct gccgaaggcg
      181 atttatatcg tttagtaggt caccacgggc cggctataga aaataaattt atggctttac
      241 gcctgtattt caatcacagc ggatcgatcg atgtatattc caagaaaata catcgtctgg
      301 aactggcgca ggctctttgg tatccgtccg aaaagatgca gcaggaacaa ggtttcggtt
      361 gcgatgagta caaagtgggt tctaccgttg gcttgggggg tatcaatctt tgggatggtc
      421 ggcaagttgt aaagctcaca gccacccagg gccgaaccgc cagggtttcc aaaaacgaaa
      481 agggagccag tatggaaatg ctggccaaag gcatagccta caaagacaaa atggtagatg
      541 ttctaatacg ggtaagtgtc aacaacacag acagacaggc cactgttgag gctgtttgta
      601 cttcgggaca taaagtacag tttcttacgg gggtaaaccg tcacccgggc aacagtgtct
      661 tcaaccggga cggccggata ggcgtctggg gcagacaccc ggcggatgtt tccaaagctc
      721 ccgttttgat aggcggaggt atgaaatacc gtccttccga tttcgctatt aagcgggtga
      781 cagacgacgc cattcagctt atatccaaac cggctaaaaa gcttaaaacc atcattgtgt
      841 ctgcgggaga gcgggaagaa gagctgaata ccgagcaggc ttttttcgat tatgtaaaga
      901 gtttctgatc taggcggaat cgggagtttg tatcactctg tttcatagaa ttctgtcatt
      961 aaagcatcaa gcccggcaat tatcgatttg ccgggcttga tgctttaatt ggaatatttc
     1021 cgaagaaatt tatttcttgt tgcggttgcc gtagcggttc aggaatttat ccacacgacc
     1081 ggcggtgtcg accaacttgg atttaccggt ataaaaaggg tgcgaagtgg aagaaatttc
     1141 cagtttgacc aaaggcaggg tttcgccgtc tatttctata gtttctttgg cttgaatggt
     1201 ggaacgtgaa ataaaggtgt caccattgga catatctttg aaaacaactg gacggtaatt
     1261 cgttggatgc aaatcttttt tcattgctca aaatattttg gctgaatgcg tcagccgggt
     1321 tttaagatta tacaaattgg taagatctgt aatggcctgc aaaagtagat atttttaatg
     1381 aaaccaaaaa aaatatcggg ctttttttct cattaagcgc aaaataccgt aattttgtaa
     1441 gcttagagat aattaaatga tcaaaatcct ttaaatacag atattatgat tagttacaaa
     1501 gaattaggtc ttgtcaactc aagagagatg ttcaagaagg ccatagccgg tcaatatgct
     1561 atcgctgcat ttaatttcaa caatatggag cagttgcagg ccattattca ggcagccgtc
     1621 gaaacccgtt ctcccgtgat tctgcaagtg tccagcggtg cccgcaagta cgccaatcag
     1681 actttgttgc gttatatggc tcagggagca gtagaatacg caaaagaact tggtctggag
     1741 catcctcaga ttgtgctgca tctggaccac ggcgatactt tcgaattatg caagtcttgt
     1801 atagatatgg gcttttcttc tgttatgatc gatggttccc atcatcccta cgaagacaat
     1861 gttgctctga ccaggaaagt ggtggactat gctcataaat acgatgttac cgtcgagggt
     1921 gagttgggcg ttttggccgg agtcgaagac gaagtgtctt cggcagtcag tcattacacc
     1981 cgtcccgacg aagtggtaga ttttgttacc agaacaggtg tggattctct ggctatttct
     2041 atcggaactt cgcacggagc caataagttc actcctgaac aatgcactcg cgatgccaat
     2101 ggtgttttaa ttccgcctcc cttgcgtttt gatattctcg aagaaattga aaagcgaata
     2161 ccgggtttcc ccattgtttt gcacggttct tcttctgtgc ctcaggattt ggttgccatt
     2221 atcaacaaat acggtggcgc catgaaagat tccataggaa ttcccgaaga acaattgcgt
     2281 agggctgcta agtcggctgt gtgcaaaatc aacatcgact ccgacggtcg tattgccatg
     2341 acggcagccg ttcgtcaggt gttggctacc aaacccgccg aattcgatcc ccgcaagtac
     2401 ctggggcctg cccgcgattc gctcaaggaa ttgtacaaac ataaattgat caatgttttg
     2461 ggttcggcca ataaactata attcagtaag attctaagcc gaaacaagat tccaagacga
     2521 attgaatcgg cgtaaaaaaa gtgagcctgg cggaaacggc acaggctcac tttttttgta
     2581 tccggcagac cgaactcccg atcagccgca attcctcttc agtaaagtgc agattatcca
     2641 atgtttgaat gttttcactc agctgtttta ccgagctggc gcctattata acggaagtta
     2701 cccgcttatc gcgcaagcac catgccaacg ccatttgagc caatgtctgt tgtctttttc
     2761 gggcgaagtc gtttagttgc ttgatttttt ctattttttc gggggtgatt tgctcttttt
     2821 tcaaaaaacc aaattcttgt ccggctcgtg aattgggagg tatcccttcc aaatatttat
     2881 cgctgagcat tccttgtgcc aaaggagaaa agccgataaa accgatgttt ttctcttcca
     2941 ccagcgggaa aatggattgc tctacttcag ggttgaaaag gctgtatctg ccctgataaa
     3001 taaggcaagg tgtaccgttt tcgctcagca aacgaatggc ttcacgggct ttgtcgggcg
     3061 gatattttga aatgccgacg tagagggctt tgccttgttt taccatatcg gacaacgcac
     3121 ccatagtctc ttccagcggc gtattcgggt cgtagcggtg cgaatagaaa atatccacat
     3181 agtccaatcc catccgttta aggctctgat taaggctggc aatcatatat ttacgcgaac
     3241 cccaatcacc gtaaggacca tcccacatca agtgtccggc tttggtactg ataatcagtt
     3301 cgtctctata ggatttaaaa tcatttttca gcattttgcc gaaatttgtt tcggcacttc
     3361 cgaagggagt accgtaatta ttcgccaggt cgaagtgggt aattccattg tcaaaagccg
     3421 taaacaacat ttttttggct tcgtcttgat cgtttacatc accgaaatta tgccagagtc
     3481 ccagtgaaat tctcggtagt tgcagtccgc tttttccgca ataattatat ccgactgagt
     3541 tcgtatatcg gtcggcagaa ggagtgtatt gcattttatt ggaagtcttg cggataacca
     3601 taaaatcttt tatctgtttt tgatacgaac tatagcaccg gtttcgggat cgagttccac
     3661 catcaactgc tcagaagccg gtaattgcat cagatctcca aattgcgcta tcacgaaatt
     3721 gtctttgaat aaaaccgtct gacctttcaa taaacttatg tcaattctgt tggggtgata
     3781 gtaatataga gcgcaggttt gttgtgatgt ttcacctttt ttaccactga tgctgtcctt
     3841 ttgttggctt atggcgtcgg taccgggatt gtgtaccaaa aaatgcaccg gatacccgct
     3901 caggtcttct ttgtctacta tccctttttg agacgaaaag cgaaacaaaa tttcgtaact
     3961 gggaatcttt tcgggtgtat attcaagcac ctctgttcga acgctttgcc tgcggcttcc
     4021 ggtaaaaagt tccgtataat gcgtttccat gcggtttagt tcggtaagca ttttttcgag
     4081 cgaagcccca tcctgaggca tagtacccaa ttctccctgc aacagactaa gccgggtgtc
     4141 tctcaattca aagacttttt tggcagccag ttcggccatg cgaatggaag agccggaaag
     4201 ttgcatatct tgggtatgaa ttgaggctgc agagctggcg ctgttgtcgt ttgcgttttc
     4261 cggacaagaa attgttgttt cttgttttga ggctttcccg gaacaggatt cagttaagct
     4321 aaggtccata tttacagccc tgagaattcc ttcggaggtg aactgaagat taagttcagg
     4381 aacttg
//
LOCUS       DAOSGL010000194         4377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_18084_length_4377_cov_3.710319, whole genome shotgun sequence.
ACCESSION   DAOSGL010000194 DAOSGL010000000
VERSION     DAOSGL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18084_length_4377_cov_3.710319"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PKY68_06530"
     CDS             complement(<1..1194)
                     /locus_tag="PKY68_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOH24726.1"
                     /translation="MLYYSRGSKNDNIGPEELRKILYEVFKRLGPKKRVLALPPDFTR
                     FHSFAGQITEMVWDYYGKTLTDVMPALGTHVPMTEKEIKTMFGNIPLELFRVHDWRND
                     VVTVGEVPASYVHEVSEGKLNYAWPAQVNKLLLDPEFDLILSVGQVVPHEVVGMANYN
                     KNIFVGTGGSEGINKSHYIGATYGMERLMGRADSPVRKVLEYASKNFTAHLPLIYIQT
                     VVSKNEEGNLVIRGIFIGDDFECFQKASDLSMEVNFLMVEKPIRKCVVWLDPSEFKST
                     WLGNKSIYRTRMALADGAELIVLAPALKEFGEDKTIDGLIRKYGYAGTPAILKAVEEN
                     EDLRDNLGAPSHLIHGSSEGRFTITYCPGHLSREEIESVYFNYGDLQEMMKRYNPEEL
                     KDGINI"
     gene            complement(1213..2064)
                     /locus_tag="PKY68_06535"
     CDS             complement(1213..2064)
                     /locus_tag="PKY68_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH24727.1"
                     /translation="MNKFTFEDLKGKVCVLTGGAGVIGASLARGLASAGVKTAIVDIN
                     EEAAVKLAAQVEKESGVKCIGISANVLDKESLEAAKSVINQKLGKIDILINGAGGNSP
                     KATTAVEQMQKEDLTDLSKTFYGLGMEGFQRVYDLNFKGTLLPTMVFTLDMLESGKGC
                     VLNISSMNAYRPLTKIPAYSAAKASVNNLTQWMSVHLAKVGIRVNAIAPGFFLTNQNR
                     FLLTDEKTGEATPRGHKIISNTPMGKYGEPEDLIGAMLYLLSDISSFVTGVIIPVDGG
                     YSAFGGV"
     gene            complement(2086..3333)
                     /locus_tag="PKY68_06540"
     CDS             complement(2086..3333)
                     /locus_tag="PKY68_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOH24728.1"
                     /translation="MKIGKYSFGTGDRFAHQGEAQLKALLKARKELGLDITPVWNKSN
                     REHMIVNSSPADTRIEADAAVKALGYNGPYFVDADHINMSNVEKFVDSSDFFTIDLAD
                     FIGKKAAQSDIDAFIKRNAKYLGKLNIPGIPEAFVITDAVLKAAAEKFLFAAQEAGRI
                     YRYIAGRKGAGNFIPEVSMDEVNDAQTPVEMFFILSALAAEKVPAQTIAPKFTGRFNK
                     GVDYVGDVAQFAKEFEQDLLVIDFAVKEFGLPDNLKLSIHSGSDKFSIYPVMGELIRK
                     YDKGIHVKTAGTTWLEEIIGLAMADEEALDLAKAIYESALGRFDELCGPYATVIDIDK
                     KQLPTPKEVEKWTGEKFANTLRHIPDNPDYNPHFRQLIHVGYKVAAEYGKEYTDALKR
                     NKAVVAEQVIANIYDRHILRMFA"
     gene            complement(3464..4165)
                     /locus_tag="PKY68_06545"
     CDS             complement(3464..4165)
                     /locus_tag="PKY68_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH24729.1"
                     /translation="MNRTILHVENDFDYAKLIGELLKKKGHNVVHASCGRKGLKPLKH
                     CSPDIVLLGIIMPDIDGFTFAEIARESDPTLPIVFLTSLSDPKNEIKGLKLGACDYIC
                     KEADLDVVLARIEKELKRCHKRYISIQLTPDTSIDTKNKMLIIRGKRHKLGMRDFDLL
                     IFLCDTIDVVHHRKIIEQTIWGENPNSTQYLYKATYNLRKILQYDPTLSIDTFRNGDI
                     VLNNTSASRIPYHVK"
BASE COUNT         1100 a          983 c         1061 g         1233 t
ORIGIN      
        1 aatattgatg ccgtctttca gctcttcggg attgtagcgt ttcatcatct cttgcaaatc
       61 gccgtaattg aagtaaacgc tttcgatttc ttcgcggctc aggtggcccg gacaataggt
      121 gatggtaaat cgcccttcgc tcgatccgtg aatcaggtga gaaggagcgc ccaggttgtc
      181 gcgcaggtct tcgttttctt ccacggcctt gagtatggcg ggggtgccgg cataaccgta
      241 tttgcgtatg agtccgtcga tggttttgtc ctcgccgaat tccttcaggg ccggggccag
      301 cacaatcagt tccgctccgt cggccagggc catacgggta cggtaaatgc tcttgttacc
      361 caaccaggtg cttttgaatt ccgagggatc gagccaaacg acgcatttgc gaatgggctt
      421 ctctaccatc aggaaattga cttccataga gagatcggag gccttttgga aacattcaaa
      481 atcgtcaccg ataaaaatac ccctgatcac taagttgccc tcttcgtttt tggatacaac
      541 ggtttggata taaatcagcg gcagatgggc ggtgaagttt ttagaagcgt attcgagtac
      601 tttgcgcacc ggcgaatcgg ccctgcccat aagacgttcc attccgtagg tggcgccgat
      661 gtagtggctc ttgttgatgc cttcggaacc gccggttccc acgaaaatgt ttttattgta
      721 attggccatg cccaccactt cgtgcggtac cacttgtccg acggacaaaa tcaggtcaaa
      781 ttccggatcc agtaagagct tgtttacctg agccggccag gcgtagttca atttgccttc
      841 ggacacctcg tgtacgtagg aagcaggcac ttcgcccacg gttaccacgt cgttgcgcca
      901 atcgtgtaca cggaacaact ctaaaggaat attgccgaac atggttttta tttccttttc
      961 ggtcatcggc acatgagttc ccagagccgg catcacatcg gtcagggttt ttccgtaata
     1021 atcccagacc atttcggtaa tctgtccggc gaaggaatgg aagcgggtga aatcgggcgg
     1081 aagtgccaac acccgctttt tggggcctaa acgcttaaaa acctcgtaca ggatctttct
     1141 caattcttcc ggaccgatgt tgtcgttttt ggagcctctg ctataataca acatacttat
     1201 atgcttttaa tactaaactc cgccgaaagc actgtagcct ccgtccaccg gaataataac
     1261 accggtaaca aaagaagaaa tatcgctcaa caggtacaac atggcgccga tcaggtcttc
     1321 gggttcgccg tattttccca tgggagtatt ggaaataatc ttgtgaccgc ggggggtggc
     1381 ttctcccgtt ttttcgtcgg tgagcaggaa acggttctgg ttggtcagga aaaagccggg
     1441 agcaatggcg ttgacacgga tacccacttt ggccaggtga accgacatcc actgggtaag
     1501 gttgttgaca gaagccttgg cagccgaata agccggaatt ttggtcagcg ggcgataagc
     1561 attcatcgag gaaatgttca gcacacaacc tttaccgctt tcgagcatgt ctaaagtaaa
     1621 gaccatggta ggaagcaagg tgcctttgaa gttaagatcg tacactcttt ggaaaccctc
     1681 catacctaaa ccgtaaaaag ttttgcttaa atcggttaag tcttcttttt gcatttgctc
     1741 aaccgcggtg gttgctttgg gagaattgcc tccggctcca ttgatcagaa tatcaatctt
     1801 acccaacttt tggttgataa cggacttagc tgcttcgagc gactctttgt ccaaaacatt
     1861 ggccgaaata cctatgcatt tcacgccgga ttctttctcg acttgagcgg ccagttttac
     1921 ggcagcttct tcgttgatat ctactatggc ggttttaaca ccggcagagg ccaggcctct
     1981 tgccaatgat gcgccaataa caccggcacc tccggtcagg acacagacct tacctttaag
     2041 gtcttcgaat gtgaatttgt tcataatata gtgtttttat ggcaattaag cgaacatgcg
     2101 caaaatatgc cgatcgtaaa tgttggcaat gacttgttct gctaccacgg ctttgttgcg
     2161 tttgagggca tcggtgtatt ccttgccgta ctcggcagca actttatagc ctacgtgaat
     2221 cagctgacgg aaatgggggt tgtaatcggg attatccgga atatgacgca gcgtgttggc
     2281 aaacttttcg ccagtccatt tttcaacctc tttgggcgta ggcagttgtt ttttgtcgat
     2341 gtcaataaca gtggcgtatg gaccgcagag ctcatcgaaa cgtcccagcg cgctttcgta
     2401 aatggccttg gccagatcca gcgcctcttc gtcggccatg gccaggccga tgatttcttc
     2461 gagccaggtg gttccggctg tcttgacgtg aatccctttg tcgtatttgc gaatcagctc
     2521 gcccataacg ggataaatgg agaatttgtc gctgccggag tgaatgctca gtttcaggtt
     2581 gtcgggcaaa ccgaattcct tgacggcaaa atcaatcacc aggaggtctt gttcaaattc
     2641 cttggcaaat tgagctacat cgcctacgta atcgactcct ttgttgaagc ggccggtgaa
     2701 tttaggcgca atcgtttggg caggcacctt ttcggctgcc agggcactca gaataaaaaa
     2761 catttctacc ggagtctggg cgtcgttgac ttcgtccatc gatacttcgg gaatgaagtt
     2821 gccggcacct ttgcgtccgg caatgtagcg gtaaatgcga cctgcttcct gggcggcaaa
     2881 aagaaatttt tcggcggctg ctttcagtac ggcatcggta atgacaaaag cttcggggat
     2941 gccggggata tttaatttac ccaggtattt ggcgttgcgt ttaatgaagg cgtcgatgtc
     3001 gctttgtgcg gctttcttgc cgataaaatc agccaaatcg atggtgaaaa agtcggaaga
     3061 atctacaaat ttttcgacat tagacatgtt gatgtggtct gcatctacaa aataaggacc
     3121 attgtagccc agggctttta cggcagcatc ggcttcgatg cgggtatctg ccggcgaaga
     3181 atttacgatc atatgttcgc ggttcgattt gttccaaacg ggagtgatgt ccaaaccaag
     3241 ctccttgcgg gctttcaaca atgctttcag ttgggcctcg ccctgatggg caaagcggtc
     3301 gcctgtgcca aaagaatatt taccgatttt catgataata aaggattaaa gggcggtcat
     3361 aagccaccgt tagtaatgaa aatattttcc acaaaaataa cggatttttt taccaaaagc
     3421 gaagcctttt caaaaaaaac aagatatcaa ccgtatttta aacttatttt acgtgataag
     3481 ggatgcggga tgcggacgta ttgttcaaca cgatgtcgcc gtttcgaaaa gtatcgatgg
     3541 agagagtagg atcgtattgc agtattttcc tcaaattgta ggtcgctttg tagaggtact
     3601 gcgttgagtt ggggttttcg ccccagatgg tttgctcaat aatttttctg tggtgaacca
     3661 cgtctatagt atcgcataaa aatatcagca gatcaaaatc gcgcattccc aatttatgtc
     3721 ttttccctct aataatcagc attttgtttt tagtatcaat ggaagtatcc ggcgtaagtt
     3781 gtatggagat atatcttttg tggcatcttt tcaactcttt ttcgatgcga gcaagtacaa
     3841 cgtctaaatc ggcttccttg caaatgtaat cgcaggctcc caatttaagg cctttgattt
     3901 catttttcgg atcagacaga gaagtaagaa acactatggg aagagtcgga tcggattccc
     3961 gcgctatttc ggcgaaggta aaaccgtcta tatcgggcat tatgatccca agtaatacga
     4021 tgtcgggtga acagtgtttg agcggtttaa gtccttttct accacaggaa gcgtgtacga
     4081 cgttatggcc tttttttttc aataattcgc cgatcaattt tgcataatcg aagtcgtttt
     4141 cgacatgaag aatggtgcgg ttcataatgt tggcattaaa catatctttt gtgaagacct
     4201 gcaaatatac ggggaaataa tggatataat agaaaaatgt tcaagaaaca gccaaaaaag
     4261 gtaaatataa ggtaaacatg tagggaaagt tgtttctaca tttgtcgcgt tttattgtcc
     4321 ttgagttcaa ataaagcagc atactcataa aaaacactta atcatgctca aacgacc
//
LOCUS       DAOSGL010000195         4367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_18132_length_4367_cov_4.208488, whole genome shotgun sequence.
ACCESSION   DAOSGL010000195 DAOSGL010000000
VERSION     DAOSGL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18132_length_4367_cov_4.208488"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKY68_06550"
     CDS             <1..637
                     /locus_tag="PKY68_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH24730.1"
                     /translation="VLFLDELPEFQRSVLEVLRQPLEDRSISIARSRYAVRYPAGFML
                     VASMNPCPCGYFNHPTKECVCTASQVHKYIHRISGPLMDRIDIQMEIVPLEYEQLSSV
                     REQESSQSIRERVIKARRLQNDRFKPDKGLFCNAQMSLKQVQRYAQAEPDAYVLLRNA
                     MEKLDLSARAYSRILKVARTIADLDASERLRTRDIAEAIQYRSLDRGTWGF"
     gene            complement(611..1144)
                     /locus_tag="PKY68_06555"
     CDS             complement(611..1144)
                     /locus_tag="PKY68_06555"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF010555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOH24731.1"
                     /translation="MIRLFLVGYMGAGKTKLGQAIAKELQLSFVDLDQYIEERRFKPI
                     PALFAEFGEEGFRKIEQGYLREVAQFENVLIATGGGTACFENNMDFMNSAGDTVYLQL
                     NAEELFKRLHRKKQNRPLLNTVNDDDLLTKIREMLSQRESFYNKAKYILPTGGLNKEE
                     SLALFKEIYSKPPSSTI"
     gene            complement(1146..2627)
                     /locus_tag="PKY68_06560"
     CDS             complement(1146..2627)
                     /locus_tag="PKY68_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HOH24732.1"
                     /translation="MSLNFITAQEAASFINHNDNIGFSGFTPAGAPKVIAQAIAEKSQ
                     AERQAGREFKVGVFTGASTGDSLDGVLARAEAVKFRTPYQSNPDLRSGINKGNIPYFD
                     LHLSQLAQELRYGYYGKINTVIIEAAGVNEKGEILLTTGVGITPTAARMADKIIVELN
                     SRHPSFINGLHDLYEPADPPHRREIPIYKPSDRIGQTLLKVDPSKIVGVVKTQLPDEV
                     KAFSEPDETTKRIGDRVAGFLVNEMRAGRIPASFLPLQSGVGNIANAVLGSLGASPEI
                     PAFEMFTEVIQDSVIEMMQSGDVKFASGCSLTITSSALDSIYENWDFFKHRLVLRPQE
                     ISNSPELARRMGLITINTALEADIFGNVNSTHVLGTKMMNGIGGSGDFTRNSYLSIFT
                     CPSTAKDGKISALVPMVSHTDHNEHSVKILITEQGVADLRGKSPMQKAQTIIENCAHP
                     DYKQLLTDYLKQTNGGNTPHNLSNCFNMHREFLDSGDMRNTKW"
     gene            2749..3306
                     /locus_tag="PKY68_06565"
     CDS             2749..3306
                     /locus_tag="PKY68_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24733.1"
                     /translation="MDNSLLDVLVIVGAVLISVIGALSKARKKTSEKEAANRAGTEAQ
                     TGADVAETQDGPHTGGIPIPDPWQELLNLPEEEDDFIPKNPETAPPAPIPEPPQKERQ
                     PLISRDIEHEPRNYIEEMAKAKAMEREKKYEQSALKYKHSVHELRVVHLDDEAESIPG
                     IDLGLDEEDAWKKAVIYHALLEPKF"
     gene            3339..>4367
                     /gene="miaB"
                     /locus_tag="PKY68_06570"
     CDS             3339..>4367
                     /gene="miaB"
                     /locus_tag="PKY68_06570"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOH24734.1"
                     /translation="MTELTLKKFYIETYGCQMNVADSEVVASILGMEAYESSTKPEEA
                     DLILLNTCSVRDNAEQKIIARLKALQAYKRKNPRLIIGVLGCMAERVKEQLILEHGAD
                     LVAGPDAYLDLPNLIRAAGLGEKAVNVELSTTETYREVLPLRLGNYRVSGFISIMRGC
                     DNFCSYCVVPYTRGRERSRDPQSILNELADLRSKGYREVTLLGQNVNSYVYEGLDFAA
                     LLSMVADAAPDMWIRFTTSHPKDMSDKLLETMASRPNTCKHIHLPVQSGSSRILKLMN
                     RRYTREDYLARVASIRSILPAASISTDLFCGFHSESAEDHQMTLSLMREVGFDSAFMF
                     RYSERPGTY"
BASE COUNT         1134 a          991 c         1099 g         1143 t
ORIGIN      
        1 ggtcttgttt ttagatgaat tgcccgaatt tcagcgctct gtactcgaag tgttgcggca
       61 accgctggaa gaccgcagca tcagcattgc ccgttcccgc tacgctgtcc gttatccggc
      121 gggatttatg ctggtggcat cgatgaatcc ctgtccctgc ggatatttca atcatcccac
      181 aaaggaatgt gtttgcaccg cctcccaagt gcataaatac atacatcgca tttcggggcc
      241 cttgatggat cgcatcgaca ttcagatgga aattgtcccc ttggaatacg agcaactgag
      301 tagcgtcagg gagcaggaaa gcagccaaag tatcagagaa agagtgataa aagccaggcg
      361 cttgcaaaac gatcgcttta aaccggataa gggattattt tgtaacgccc aaatgagttt
      421 aaagcaggtg caacgctatg ctcaggcgga gcccgatgct tatgttttgc tgagaaatgc
      481 catggaaaaa ctggatcttt ctgccagagc ttacagccgt atccttaaag tagcccgcac
      541 cattgcagat ctggacgctt cggaacgctt gcgtacacgc gacattgccg aagctataca
      601 ataccgcagc ctagatcgtg gaacttgggg gttttgaata aatttctttg aaaagcgcca
      661 gggattcttc tttgttgaga cctccggtag gcagaatgta tttggcctta ttgtaaaaac
      721 tttccctttg agagagcatt tctctgattt tagtgagcaa atcgtcgtcg ttgacagtgt
      781 tgagcagggg tctgttttgt tttttccgat gcagcctttt gaacaattct tcggcattga
      841 gctgaagata gacggtgtcg ccggcagagt tcataaaatc catgttgttt tcgaaacaag
      901 cggtacctcc cccggtggca atcaatacat tttcgaattg ggccacttct cgcagatagc
      961 cttgttcgat tttgcgaaaa ccttcttcgc cgaattcggc aaaaagtgcc ggtatgggct
     1021 taaagcgccg ttcttcgatg tattgatcca agtcgacaaa agaaagttgc agttctttcg
     1081 caatggcttg tcccaacttg gttttgccgg cacccatgta accaaccagg aataaccgga
     1141 tcattttacc acttggtgtt gcgcatgtcg cccgagtcga gaaattcccg gtgcatatta
     1201 aagcaattgc tcagattatg gggagtattt ccgccattgg tctgtttaag gtaatccgtc
     1261 agcaattgtt tgtaatcggg atgagcacaa ttctcgataa tggtttgcgc tttctgcatg
     1321 ggagatttgc ctcttaagtc ggcaactccc tgctcggtta ttaaaatttt caccgaatgt
     1381 tcgttgtggt cggtatgcga aaccatagga acaagggcgc tgattttgcc gtctttggca
     1441 gtcgagggac aagtgaagat ggaaaggtag gaattgcggg taaaatcgcc cgagcctcca
     1501 ataccgttca tcatcttagt gcccagtacg tgggtgctgt ttacgttgcc gaaaatgtcg
     1561 gcctccagag ccgtgttgat ggtaatcagc cccatgcgac gagccagttc gggagaattg
     1621 gaaatttcct gaggccggag gaccagtcta tgcttgaaaa agtcccagtt ttcgtaaatg
     1681 gaatcaaggg ccgaacttgt aatggtaagt gaacagccac tggcaaattt tacatcgccg
     1741 ctttgcatca tttcgatcac ggaatcctgt ataacttcgg taaacatttc gaaagcggga
     1801 atttcggggc tggcgcccaa tgaacctaat accgcattgg cgatattgcc tacgcccgat
     1861 tgcaaaggaa gaaaggaagc aggaatacgg cctgctctca tttcgttgac cagaaaaccg
     1921 gctacgcgat ctccgatgcg tttggtggtt tcgtccggtt cggagaatgc cttaacttcg
     1981 tcgggtaatt gagttttgac tacaccaacg attttggagg gatctacttt caagagagtt
     2041 tgtccaatgc ggtcgctggg tttgtaaatg ggaatttcgc gacgatgggg aggatcggcc
     2101 ggttcgtaaa gatcgtgcaa accgttgata aacgaagggt gccggctgtt gagctcaaca
     2161 atgattttgt cggccatgcg ggctgccgtg ggagtgatac ccactccggt ggtaagtaga
     2221 atttcgcctt tttcgttaac gccggcagct tcgattatga ctgtgtttat ttttccgtaa
     2281 tatccgtagc gtagttcctg agccaattgc gagaggtgaa gatcaaaata gggaatgttc
     2341 cccttgttga taccactccg taaatccgga ttggattgat aaggtgtgcg aaatttcaca
     2401 gcctctgcgc gggcgagcac accgtcgagc gaatcaccgg ttgaagctcc tgtaaacaca
     2461 ccgactttaa attcccgtcc ggcttgccgt tcggcttgcg atttttcggc tatggcttgt
     2521 gcaatgactt taggtgctcc ggcaggagta aaaccactaa atcctatatt gtcgttgtga
     2581 ttgatgaaag aagctgcttc ctgagcagta atgaaattaa gagacatgat actatattgg
     2641 aaaaacggcg caaaggtaca taaattaaca aaatcgacca aaacaggttt acatcttttt
     2701 gtaattcccc gggaaaacac tacttttgga caacaaattt cggacactat ggacaattcc
     2761 ttattagatg tgttggtgat tgtcggagca gtactgattt cggtaatcgg tgccttatct
     2821 aaagcccgca aaaagacttc agaaaaagaa gctgcaaacc gagccggtac agaagctcaa
     2881 accggagccg atgtagcaga aactcaagac gggccccaca ctggaggaat ccccatcccc
     2941 gacccctggc aggaattatt aaatcttccg gaagaggaag acgactttat acctaaaaat
     3001 cccgaaactg ccccgcctgc tccaatcccc gaacctcccc aaaaagaaag gcaaccctta
     3061 atcagtcgcg atatcgaaca tgagccccgt aattatatag aagaaatggc caaagccaag
     3121 gccatggagc gagaaaaaaa atacgagcaa tccgccttga aatacaagca tagcgttcat
     3181 gaactcagag tagttcatct cgatgacgaa gccgaaagta ttcctggaat tgatttaggc
     3241 cttgatgaag aggatgcctg gaaaaaggca gttatttacc atgccctgtt ggagccgaaa
     3301 ttttaagatg aattttacgg aacctttcag gcttttcaat gacagaatta accttgaaaa
     3361 aattttatat cgaaacctat ggttgccaaa tgaatgtggc agacagcgag gtggtggctt
     3421 ctattctggg gatggaggcc tatgagagtt cgacaaaacc cgaagaggcc gatctgattt
     3481 tgctcaacac ttgttccgtc cgcgataatg cggagcaaaa aataatagcc cgcctgaagg
     3541 cattgcaagc gtacaagcgt aaaaatcccc gtctcatcat aggggtgctg ggttgtatgg
     3601 ccgagcgagt caaggagcaa ctgattttgg aacacggagc tgatctggtg gccggtcccg
     3661 acgcttacct cgaccttccc aatttaatta gagcggccgg gttgggggaa aaagcggtta
     3721 atgtggaact ttcgaccaca gaaacgtatc gggaagtatt acccctcagg ttggggaatt
     3781 accgggtttc gggttttatt tccatcatgc ggggctgcga taatttttgc agctattgcg
     3841 tggtgccgta tacccgtggg cgagaacgca gccgcgatcc gcaaagtatt ctgaatgagc
     3901 ttgccgactt gcgctccaaa ggctacaggg aagttacact gctgggacaa aatgtgaatt
     3961 cttatgttta tgagggactt gattttgccg cattgctgtc gatggtagcc gatgcggctc
     4021 ccgatatgtg gatccgtttt accacctcac atcccaagga tatgagcgat aaactactgg
     4081 aaacgatggc ttcgcgtccc aacacttgca agcacattca tttacctgtg caatcgggca
     4141 gttcgcgtat tctgaaactc atgaaccgcc gttacacccg cgaggattac ctggcaaggg
     4201 tggcttctat tcgttcgata ctccctgcag cttccatcag taccgacttg ttttgcggtt
     4261 ttcactccga gagcgccgaa gaccatcaaa tgaccttgtc gttgatgcgt gaagtgggtt
     4321 ttgattcggc gtttatgttt agatattccg aacgcccggg tacgtat
//
LOCUS       DAOSGL010000196         4319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_18399_length_4319_cov_4.639775, whole genome shotgun sequence.
ACCESSION   DAOSGL010000196 DAOSGL010000000
VERSION     DAOSGL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18399_length_4319_cov_4.639775"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="PKY68_06575"
     CDS             <1..163
                     /locus_tag="PKY68_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HOH24735.1"
                     /translation="RKAFENSMEGKGTSIVEIVSTCNSGWKMSPVQANQWMVENMIPF
                     YPLGDLKDK"
     gene            168..704
                     /locus_tag="PKY68_06580"
     CDS             168..704
                     /locus_tag="PKY68_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH24736.1"
                     /translation="MKEEIIISGFGGQGVLSMGKILAYSALLENKEVSWMPAYGPEQR
                     GGTANVTVIISDELISSPILNSFDTAIILNQQSMDKFEPKIKPGGVLLYDPHGITRAP
                     VRKDIRIHTIDATEIASDMDNSKAFNMIVLGAYLKVLPIVQLKSILEGLRKTLPARHH
                     KLIPMNEQALQAGMDKVK"
     gene            complement(771..1643)
                     /locus_tag="PKY68_06585"
     CDS             complement(771..1643)
                     /locus_tag="PKY68_06585"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOH24737.1"
                     /translation="MKFRLLLIATLFSITLFAQEKTEEAKSPWSYKGITGLNFSQTSF
                     TNWSEGGENSVAGNVYLNASLNYVHNKVSWTNDLSTNFGMYYTTENNWRKNIDNINFA
                     SKYGRSISKALYYAALLDFKTQFANGYKYAVSTTDRVSGFMTPAYLNLSVGVDYKPNE
                     HFSAYYSPVTGKMTFVSDTTFSTNYGLQAGKKNRAELGSSFRASANYDIFKVVNLKSS
                     LDLFTAYNQSFGKIDVNWEVLLNLKVTELLTVTFQSTLRFDDDIKYVDANGVLGPARV
                     QFKEVLGVGLSYKF"
     regulatory      1754..1945
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2153..2905
                     /locus_tag="PKY68_06590"
     CDS             2153..2905
                     /locus_tag="PKY68_06590"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOH24738.1"
                     /translation="MNTKHLVRSFTLGLTLIGMLACEPVVSKHEEEICIDFEQPVIEN
                     FQNNQVYAAKGLVFNNRYTLSEYEWEGKTYTSETWYGFSLSAMCDTVTEGFGNQYSCS
                     AGKAAGGKQFGLFYYSAYDADSSCFVRFEEEKEYVIKGMQITNSTYAYLSMKNGDDFA
                     KKFEAGDYLRIIFKSYDAEGKYLAQKTFYLADFRENKKTLLNSWEFVDLRELGKLNKL
                     SIFFESSDTGMYGINTPTYVCVDNLIYIKVQE"
     gene            3023..3706
                     /gene="trmD"
                     /locus_tag="PKY68_06595"
     CDS             3023..3706
                     /gene="trmD"
                     /locus_tag="PKY68_06595"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOH24739.1"
                     /translation="MLRIDIITVLPELLEGPLSCSIIQRARDKKLVDINVHCLRDYST
                     DLKHRRVDDYAFGFSAGMVLQIEPVDRIITHLCSQRKYDEIIYTSPDGEKFTQRLANQ
                     LSLKQNLIVLCGHYKGIDHRIREHLITKEISIGDYVLTGGELAAAVITDCVVRLIPGA
                     LGDEQSALSDSFQDQLLAPPIYTRPAEYKGWKVPDILLSGNQRKIQEWEMEQSLERTQ
                     RLRPDLLDE"
     gene            complement(3857..>4319)
                     /locus_tag="PKY68_06600"
     CDS             complement(3857..>4319)
                     /locus_tag="PKY68_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOH24740.1"
                     /translation="KEVRAVYKKHLMLKLSPNVTDITELAKIAEDQGADSLSLINTLL
                     GMAIDAENRRPKLSTVTGGLSGPCIKPVALRMVWQTYKAVKIPVIGIGGISNTADAIE
                     FMLAGASAIQVGTANFIDPGVSVSIAEGIADYCRQHGFKSAKELTGALIID"
BASE COUNT         1265 a          910 c          933 g         1211 t
ORIGIN      
        1 ccgtaaagct tttgaaaatt ctatggaggg caaaggcacc agcatagtag aaatcgtttc
       61 tacctgtaat tcgggctgga aaatgagtcc ggtacaggct aaccaatgga tggttgaaaa
      121 tatgattcct ttttacccct tgggcgactt aaaagacaaa taaaaaaatg aaagaagaaa
      181 taattatatc cggctttggg ggacaaggcg ttttgtccat ggggaaaatc ctggcttatt
      241 cagccctgtt ggaaaacaag gaggtcagct ggatgccggc ctacggcccg gaacaaagag
      301 gcggaacagc caacgttact gtcattatca gcgacgaact tattagttca cccattttaa
      361 attccttcga tacagccatc atacttaacc agcagtcgat ggataagttt gaacctaaaa
      421 tcaagcccgg aggggtactt ttatacgatc ctcacggtat tacccgggct ccggtgcgca
      481 aagacatccg gattcatacc atagacgcta cggaaattgc ctccgatatg gataatagta
      541 aggctttcaa catgatcgtg ctgggagctt acttaaaagt cctgcccatt gtgcagctga
      601 aatccatctt ggaaggttta agaaagacat tgcccgcaag gcaccataag ctcattccca
      661 tgaacgaaca agccttgcag gcgggaatgg ataaagtgaa gtaaaccaaa ttgcccggga
      721 agccttcaaa ttcgtctcgc tcagataacg ggatgcacga ctatgcagac ttagaattta
      781 taggacaagc ccacaccaag cacttcttta aactgtactc tggccggacc taaaacaccg
      841 ttggcgtcta cgtatttgat gtcgtcgtcg aaacgcaggg tggattgaaa agtaaccgtg
      901 agcagttccg taactttaag gttgagcaac acttcccagt tgacgtcaat tttaccgaaa
      961 ctttgattgt aagcagtaaa caaatccaga ctggatttaa ggtttaccac cttaaaaata
     1021 tcgtaattag ctgaagctct aaagctacta cccaactctg cacggttttt cttaccggct
     1081 tgcaggccat aatttgtcga aaaagtagta tccgaaacaa aggtcatttt gccggtaacg
     1141 ggcgaataat aagcactaaa atgttcattg ggtttgtaat ctacacccac tgaaagattc
     1201 aggtaagcag gagtcataaa gccggaaacc ctgtcggttg tactgacggc gtatttgtag
     1261 ccattagcaa actgggtttt aaaatccagt aaggcagcat aataaagggc ttttgaaata
     1321 ctacgtccgt atttggaggc aaaattgatg ttatcaatat tttttctcca gttgttttcg
     1381 gtggtgtaat acataccaaa attggtactc aaatcgttgg tccacgatac tttgttatga
     1441 acgtaattta gagaggcatt caaatacaca ttacctgcca ctgagttttc gccaccttca
     1501 ctccaattgg taaaggaagt ttgggaaaaa tttaaaccgg taataccctt gtacgaccag
     1561 ggacttttgg cttcttcggt tttttcctga gcaaacaaag ttatcgaaaa caatgttgct
     1621 attagaagta atctgaattt catacacttg gtgattaacg agtaaatagg ttttttttaa
     1681 cggctgcaaa tgtaagcaaa ataaagctgt gaaaaaatcc ttcatttgtt tttatcctca
     1741 tacaaaattg actagatttg cggcatcaac tggtgatgcc tgtcctttcg ggaccggcat
     1801 aaaagggaat ccggtgtaaa tccggaacag tgcccgctgc tgtaagattc ttacaaggct
     1861 ttgtttctgc aaaccactgt ctttgtgacg ggaaggtgac aaggtgaatc aagtcagaaa
     1921 acctgccggt tactattttc aacgctttcg ggaacaaaag ctgaaaaagt tttcccaggg
     1981 atcccttttt cttctttatg cccggtcacg cacgcggtgt catcgtgttg ttgaaaatca
     2041 ttgtgcaagc agggcaaaca caataaaaca tttcaggctt ctgtaaacgg atggggtttc
     2101 tgcgttttat ggagtcattt tttgcaggaa cccacttatt ataattttaa ttatgaatac
     2161 aaaacacctt gtccggtctt tcacattagg cctgactttg attggaatgt tggcttgtga
     2221 gcctgtggtc agtaagcatg aagaagaaat ctgcatcgat tttgagcaac ctgttattga
     2281 aaattttcaa aacaatcagg tttacgccgc caagggactg gtttttaaca acaggtatac
     2341 cctttcggaa tacgaatggg aaggtaaaac ttacacttca gaaacttggt acggtttctc
     2401 tttgagcgct atgtgcgata ctgttactga gggctttggc aatcaataca gttgtagtgc
     2461 aggtaaagct gctggaggca aacaattcgg cctcttttat tacagtgcct acgatgccga
     2521 ttcttcttgt tttgttcggt tcgaagaaga gaaggaatac gtaataaaag gcatgcaaat
     2581 cacaaattcc acctatgctt atctttcgat gaaaaatggg gatgattttg caaagaaatt
     2641 tgaagcaggt gattatctgc gaattatatt taaatcatac gatgccgagg gaaaatattt
     2701 agctcaaaaa accttttatc tggccgactt cagagagaac aaaaaaactt tattgaattc
     2761 ctgggaattt gtcgatctga gagaattggg caaactaaat aaactcagta tattttttga
     2821 gtctagcgac accggcatgt acggcatcaa tacaccgacc tatgtctgtg ttgacaattt
     2881 gatttacata aaagtacaag aataactgtc tgccggcaaa cttgtactgc tccgcagaag
     2941 ttgctttggc tgtcaaagta taaaacaaga ctccatttag cttatgcgga gcatttttgt
     3001 atctttgcgc caaaatacga ccatgttacg cattgatatt attacggttt tacctgagct
     3061 tttggaaggg cccttaagtt gttccataat acaaagagcc agagataaga aactggttga
     3121 catcaatgta cattgtttac gtgattattc caccgatcta aagcatcgca gggttgacga
     3181 ctatgctttc ggtttttcag caggcatggt attgcaaatt gaaccggtag accggatcat
     3241 cactcatctg tgtagtcaac gcaaatacga tgagatcatt tatacttcgc ccgacggaga
     3301 aaaattcact caaagactgg ccaatcaact ttccctgaaa cagaatttga tcgtcctctg
     3361 cgggcattac aaaggcatcg accatcgcat acgtgaacat ctgataacca aagaaatatc
     3421 tattggcgat tatgtgctca ccggtggaga actggcagcc gccgtaataa cggattgcgt
     3481 cgtgaggctt atcccgggag ctttgggtga cgaacaatct gccctctcag attcctttca
     3541 ggaccaattg ctggcaccgc ctatttatac cagacccgca gaatataagg gctggaaagt
     3601 cccagacata ttattgtcgg gaaaccaacg taaaattcag gaatgggaaa tggagcaatc
     3661 tttggaaaga actcaacgtc tgcggccgga tttgcttgac gaatgatctt cgaatttcgc
     3721 taatatactg ctgctcaacg cacacctagt cgtttcagcc tacccgctat tggtttgcat
     3781 taatcaagtt taagccgcct tgagcgacgc catgaaaaca attccgtcag ctcgtctgcc
     3841 tgaaattcct tgtgcactaa tcaattatga gggctccggt cagttctttg gccgatttaa
     3901 aaccatgttg ccgacaataa tccgctatgc cttcggctat gctgaccgag actcccggat
     3961 ctataaaatt agcggttccc acttgtatgg cagatgcacc ggccaacata aattctatgg
     4021 catcggctgt atttgagatg cctcctatgc ctataacagg gattttgacc gccttatatg
     4081 tttgccaaac cattctgaga gcaacaggct tgatgcaggg tcctgacaaa cctcccgtca
     4141 ccgtggaaag cttaggacgt ctattttctg catcaatggc cataccaagc aaagtattaa
     4201 tcaaagaaag agaatcggct ccctggtctt cggctatttt ggcgagctcc gtgatatcgg
     4261 ttacattggg cgataatttc aacatcagat gctttttata aactgccctg acttctttg
//
LOCUS       DAOSGL010000197         4289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_18567_length_4289_cov_4.155172, whole genome shotgun sequence.
ACCESSION   DAOSGL010000197 DAOSGL010000000
VERSION     DAOSGL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18567_length_4289_cov_4.155172"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKY68_06605"
     CDS             <1..574
                     /locus_tag="PKY68_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOH24741.1"
                     /translation="KDGEMHYDIVSAFIKSIRGSDPDAAVYWLARMVEGGEDPKFIAR
                     RMLISASEDIGLANPNALLLANACFDAIHKIGWPESRIILSETAIYLASSPKSNSAYL
                     AIEKASSIVRQTGNVSVPLHLRNAPTQLMRDLDYGKDYKYAHDYRDNFVEQDYLPPEI
                     KGKSIWEPQQNAAENKLKEFLKRLWKNRFQ"
     gene            complement(658..1566)
                     /locus_tag="PKY68_06610"
     CDS             complement(658..1566)
                     /locus_tag="PKY68_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOH24742.1"
                     /translation="MALSEDNHFNYLTVNETDQKWGLYVTTCGFQSIKPHSSINPYLN
                     KHLSSYAFNTKQGRVLDEFQLLFVVKGSGFFQSASVPKTNIETGSIIMLFPQEWHNYS
                     FSKETGWDEFWVGFSGEHARSIVHQGFFRKEEPIFKLGINDEFVSLYRQLISYAEQEN
                     QAYQQIISGIVRHMLGLLYYKSRNVVVSNKSVVDKINRARIMMRENVSSPQAPEEIAS
                     QLGVSYSWFRKAFKKYTGISPAQFQMNLRLQHAKDLLMNSDKSITEIAYELNFESTGQ
                     FSTFFKHKTGTTPREFRSVASAPGPH"
     gene            1694..3811
                     /locus_tag="PKY68_06615"
     CDS             1694..3811
                     /locus_tag="PKY68_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOH24743.1"
                     /translation="MKRQIYAFKIEFQALNFKLRFLLLLLFFFVLESNGQNPFFKASE
                     LSQTWVYYYPEHWPENQWERDFKNMAGLGFEAVHMGEFAWAQLEPEEGRYHFEWLDRA
                     IDLADKYQLKVILCTSTATPPVWMSRKYPEILIKHENGNYSDHGARQHASFSSPKYRE
                     LALRTIEELAKRYGSDTRVIGWQLDNEPSVQYDYNPAAAAEFRTFLQNKYQTIDKLNQ
                     AWGTAFWSQLYTSFDQINIPLMKQQFMNHHQILDYMRFAAQQTASFLDMQSRCLKKHI
                     TVEQFVTTNYIPNYEEGYIGLCKDLDFHSYTRYMVFGENKGLGRKGFRVGEPLRIALA
                     NDFFRPIDNLYGVMELQPGQVNWGSVNSQPLPGAVRLWLWSVFSGGSRFVCTYRYRQP
                     LYGTEQYHYGIVGTDGLSLSSGGKEFCTYMEELELLRKQYDRPRGELDYLSKAAILYN
                     HENAWSIRNQKQNQHWNTEKHILSYYSALKSFGSKVDVITESADFEHYPLIIAPAYQL
                     IDDELVERWKKYVEAGGHLVLTCRTGHKNRNGSLFEAKFGEKINELSGTEMLFYDLLP
                     PEAADSVCMGTKSYAWYTWGEVFEPAPGTEVWAHYRGDFYSGKAAVVHKKHGKGSVTY
                     VGVDSRDAELEKAVLIKLYERLGLSRLDLPPGVVVEYRDNFGIATNYSDKNYEFPLPA
                     RSEILIGEKELKTAGVLIWKQKK"
     gene            3827..>4289
                     /locus_tag="PKY68_06620"
     CDS             3827..>4289
                     /locus_tag="PKY68_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOH24744.1"
                     /translation="MKKLIFLLLLLPALLACQKIDSQNLPDRNKVLALMELANSHFMQ
                     KWPDVAQETFVRNRYRPSHIWTRGVYYEGLMALHQICPRDEYFRYAYDWGAAHNWELH
                     RGNTTRNADNHCAGQTYIALYNICGDPALIKNIKISMDMLVNTPQNNDWSWI"
BASE COUNT         1218 a         1056 c          965 g         1050 t
ORIGIN      
        1 caaagacgga gaaatgcatt acgacatcgt ctctgcattt ataaaatcca tacgaggcag
       61 cgatccggat gctgccgttt actggctggc caggatggtc gaaggaggcg aagaccccaa
      121 attcattgcc cgccgcatgc tgatttccgc ctccgaagac attggtctgg ccaatcccaa
      181 cgccttgtta ctggccaacg cctgtttcga tgccatccat aaaatcggct ggcccgaaag
      241 ccggattatc ctttcggaaa ccgctattta tctggcatcc agccccaaaa gcaattcagc
      301 ctacctggcc atagaaaaag cttcgtctat agtgcgtcaa acaggcaacg tcagcgtacc
      361 gctgcatctg agaaatgctc ccacccaact tatgcgagac ctggattacg gcaaagacta
      421 caaatacgcc cacgattacc gtgacaactt tgtcgaacaa gactacctgc ctcccgaaat
      481 caagggcaaa agcatatggg aacctcagca gaatgccgcg gaaaacaagc tgaaagaatt
      541 tctgaaaagg ctctggaaaa acaggtttca ataaaaaccc ggtattaact ataacaggta
      601 gggaccggag aaatggactc cttgttatca acaacactag tatcaacggt aacaggctca
      661 atgaggcccc ggggcagaag caaccgaacg gaattccctg ggcgtggtgc cggttttatg
      721 cttgaaaaag gtagaaaatt gccctgtact ctcaaaattg agttcgtaag caatttcggt
      781 gatggattta tcgctgttca tcagcaaatc tttggcatgt tgcaaacgca ggttcatctg
      841 aaactgagcc ggcgaaatac ctgtgtattt tttaaaggct tttctgaacc aggaataact
      901 aacccccagt tgcgaggcga tttcttcggg agcctggggc gaagacacat tttcacgcat
      961 cattatcctg gcccggttga ttttgtctac taccgattta ttactgacga cgacattacg
     1021 cgatttgtaa tacagcaaac ccaacatatg cctgacaatt ccggaaataa tttgctgata
     1081 ggcctgattt tcctgttcgg cataactgat gagctgccgg taaagagaaa caaactcatc
     1141 gttgatgcct aatttgaaaa tgggttcttc tttccggaaa aaaccttgat gcacaataga
     1201 ccgggcatgc tctccgctaa aacccaccca aaattcatcc cagcctgttt ctttactgaa
     1261 actgtaatta tgccactcct ggggaaaaag cataattatg cttcctgtct ctatatttgt
     1321 ttttgggacg gaggccgatt gaaaaaaacc actgcctttc actacgaaca agagctgaaa
     1381 ttcatccaac acccgacctt gttttgtatt gaaagcgtag gagctgaggt gtttgttcaa
     1441 ataaggatta atactggaat gcggtttaat gctttggaat ccgcaagtgg tcacataaag
     1501 cccccacttt tgatctgttt cattaaccgt caagtaatta aaatgattat cttcgctcaa
     1561 tgccatcgta ttaaaattct agttaagcgc ctaaatgtca attttataag aatttatgtc
     1621 attatttgca aacaagtttt cgttcaggtg cttatttttg tttactttgt gacccttatt
     1681 tttaataccg gaaatgaaga gacaaatata cgcttttaag attgaatttc aagctttaaa
     1741 ctttaaatta aggtttttgc ttttactgct cttcttcttt gttttagaaa gcaatggcca
     1801 aaaccctttc ttcaaagcga gcgaacttag tcaaacctgg gtgtattatt atcccgaaca
     1861 ctggcccgaa aatcaatggg aaagagattt caagaacatg gccgggctgg ggtttgaagc
     1921 cgtccatatg ggcgaatttg cctgggctca actggaaccc gaagaaggcc gttatcactt
     1981 tgaatggctg gacagagcca tcgatctggc cgataaatac caactcaagg taattttatg
     2041 cacctccaca gccactcctc cagtctggat gagcagaaaa taccctgaga ttctgatcaa
     2101 gcacgaaaat gggaattact ccgatcacgg tgcccggcaa cacgcctctt tttccagtcc
     2161 caaataccgg gagctggcgc tcaggaccat cgaagaactg gctaaacgtt acggcagcga
     2221 caccagagtg atcggctggc aactcgacaa cgaaccctcg gtgcaatacg actacaatcc
     2281 ggccgctgcg gcagaattca gaacttttct gcagaataaa taccagacca tcgacaagct
     2341 aaaccaagcc tggggtacgg ccttttggag ccaactgtac accagtttcg accagatcaa
     2401 catccccctg atgaaacaac agtttatgaa ccatcatcag atcctggatt atatgcgttt
     2461 tgccgctcaa cagacggcct cctttttgga catgcaaagc cgctgtctga aaaagcacat
     2521 caccgtggaa caatttgtaa ccaccaatta tattcccaat tacgaagaag gttatattgg
     2581 cttgtgcaag gatttggatt ttcattccta cacccgttat atggtctttg gcgaaaacaa
     2641 aggtctgggc cgcaaaggtt ttcgtgtggg agagcccctg cgcattgccc ttgccaacga
     2701 cttcttccgg cctatcgaca acctttacgg tgtgatggag ctccaacccg gccaggtaaa
     2761 ctggggcagt gtcaacagtc agcccttgcc cggagcggtg cgtttgtggt tgtggagtgt
     2821 cttttcggga ggcagccggt tcgtgtgcac ttatcgttac cgacaacctt tgtacggaac
     2881 ggagcaatac cactacggca tcgttggtac cgatggcctg agcctgagca gcggaggaaa
     2941 agaattttgt acctatatgg aggaactgga actgcttcgc aagcaatacg accggccacg
     3001 cggagaactc gattatctct cgaaagcggc catactgtac aatcacgaaa acgcctggag
     3061 catccgcaat caaaaacaaa atcaacactg gaacacggaa aaacatattt taagctatta
     3121 cagtgccctg aaatcgtttg gttccaaagt agatgtcatc accgaatcgg ccgatttcga
     3181 acactatcct ctgataatag ctccggcata ccagttaatt gacgacgaac ttgtggaacg
     3241 ctggaaaaaa tatgtagaag ccggcggtca cctggttcta acctgccgta ccggacacaa
     3301 aaaccgcaac ggatccttgt tcgaagccaa attcggtgaa aaaatcaacg aactgagcgg
     3361 aaccgaaatg ctcttctacg atttgcttcc gcccgaagcc gccgactcgg tctgcatggg
     3421 gacgaaatcc tatgcctggt acacctgggg agaagtgttc gaacctgctc cgggaaccga
     3481 agtctgggct cattaccggg gtgattttta ttccggaaaa gcagccgtcg tgcacaaaaa
     3541 acacggtaag ggcagcgtta cctatgtggg cgtagacagc cgcgatgccg aactggaaaa
     3601 agcggtgttg attaaattat acgagcggct gggcctgagc cgtctggatc tgcccccggg
     3661 agttgtagtg gaataccgcg acaacttcgg catcgccacc aattacagcg ataaaaacta
     3721 cgagtttccg ctgccggccc gttctgaaat actgatcgga gaaaaagagc tgaaaacggc
     3781 cggtgtattg atttggaaac aaaaaaaata ataaacaata agcactatga aaaaactgat
     3841 ctttctgctt ctcctgttgc cggccttact ggcctgtcaa aaaatcgatt cgcaaaattt
     3901 gcccgacagg aacaaagttc tggcccttat ggaattggcc aacagccatt ttatgcaaaa
     3961 atggcccgat gtagcccagg agacttttgt acgtaaccgc taccgtccca gccatatctg
     4021 gaccagaggc gtatactacg aaggcctgat ggctttgcac cagatctgtc cccgcgatga
     4081 atatttccgt tacgcctacg actggggtgc ggctcacaat tgggaactac accggggcaa
     4141 cactacccgg aatgccgaca atcattgcgc cggccagacc tacattgctt tatacaatat
     4201 ttgcggtgat cctgccctga taaaaaacat caaaatctct atggacatgc tggtcaacac
     4261 ccctcaaaac aacgactggt cctggatag
//
LOCUS       DAOSGL010000198         4264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_18718_length_4264_cov_3.361844, whole genome shotgun sequence.
ACCESSION   DAOSGL010000198 DAOSGL010000000
VERSION     DAOSGL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18718_length_4264_cov_3.361844"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /gene="rplJ"
                     /locus_tag="PKY68_06625"
     CDS             complement(<1..183)
                     /gene="rplJ"
                     /locus_tag="PKY68_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOH24745.1"
                     /translation="MASKEHLTEKQAVVDEIKAKLEKAQSAVVIDYIGITVAEADAMR
                     KKLREANVDYTVYKNTL"
     gene            217..1995
                     /locus_tag="PKY68_06630"
     CDS             217..1995
                     /locus_tag="PKY68_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOH24746.1"
                     /translation="MEMIELSIDNKVLRVKKGTTILKAARKVGIEIPTLCHMELHNMN
                     IENKPGSCRVCVVEVDGRRNLAPACITEVTPGMVVNTHTVRVLNARKTIVELLISDHP
                     YDCLVCAKSGNCDLQSIAHKFGIRRVHFKGEKSTYRKDTSPSIIRDVDKCIMCRRCEM
                     MCNEVQTVGALSAVNRGFISVVAPAFEMNLEQSSCTYCGQCVSVCPTGALTEVDSTNK
                     VLLALMDKSKTVIVQTAPSVRAALGEEFGLPAGTLVTGKMVAALRRIGFKYVFDTDFA
                     ADLTIMEEGTELLGRLKAHLAGESNAKLPILTSCCPGWVNLMEHNFPDLLHIPSTAKS
                     PQQMFGAIAKTYFADLIGVKRQDLVVVSVMPCLAKKYECQRDEFKVDGNPDVDFSIST
                     RELADLIKQSNIDFLNLPDEDFDTPLGESTGASVIFGNTGGVIDAACRTAYELYTGKK
                     LDVIEFESLRGKEGIRSAKLDFDGLTLNIGIAHGLSNARKLLDDVRAGKSDYHAIEIM
                     ACVGGCIAGGGQPYHHGDSKILRQRRNAIYREDKNKTIRKSHENPYIIQLYKDFLGQP
                     CGQKSHELLHTVYYDKSREEITIHHD"
     gene            2058..2618
                     /gene="nuoE"
                     /locus_tag="PKY68_06635"
     CDS             2058..2618
                     /gene="nuoE"
                     /locus_tag="PKY68_06635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOH24747.1"
                     /translation="MADVHAPATSDKDSLKVTIKLSVEKINAINRICASYDNDPEELI
                     SVLHETQNTLGYLPAEVQEIIAHNLKISAAHVYGVVTFYSYFTMVPKGDHPISVCMGT
                     ACYVRGAEKILDELKKELNINIGETTPDQRFSISCLRCVGACGLAPVMMVGEKVYGRV
                     SPNDVRKILEEYREDNCEECKPTQQL"
     gene            complement(2637..3599)
                     /locus_tag="PKY68_06640"
     CDS             complement(2637..3599)
                     /locus_tag="PKY68_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOH24748.1"
                     /translation="MKTRKIIGYSLLFLICISFSSLLHGQAANTITTTDSSQLITDTL
                     ASQTALPPVAERGNLAQELTQAPDVSGLISFPKIFWSVVFLLLGYFSLKILTGILNLY
                     AERKPRHKSLVRRVLPMIKILVWSMVVYIIVSGIFSPPKETVLAFFASIGVAIGFASQ
                     DLLKNLFGGLMILFDKPFQIGDRIETGKYYGEVVEIGIRSTRIVTPGDNIVSVPNSEI
                     MNQSISNTNSGLNNCLVSADIYLPHLIDTQRVRQIAIEVAQISKYVYLNKPIAVIFFS
                     EKTNKEPVLKMRLNAYVMNVGYEFLFKSEMTELVLRELKNEGLM"
     gene            complement(3623..>4264)
                     /locus_tag="PKY68_06645"
     CDS             complement(3623..>4264)
                     /locus_tag="PKY68_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24749.1"
                     /translation="IISTSSEKVFWICSCGQYSADYLNKTIGLFGYFPVLIQMQALEL
                     EKIIKIILLRHRASGYDLSYMPSNRDLADKKFTKMEESKQQEVLETKYFSMLNQITES
                     NISFALLLWLASIHSIDNNRVYLESLDKLDFSFLYNQPREAVFALHALLLHESLNPEE
                     LAAVLNCPQRQAALLLMRLNDRGIVLDEDGFYKIHPLLYRSTIQMLRDNNLIY"
BASE COUNT         1261 a          937 c          921 g         1145 t
ORIGIN      
        1 gagagtgttt ttatatactg tataatcaac gttagcttct ctcagctttt ttctcattgc
       61 atcggcttct gcaacggtga ttccgatata atcgattact accgccgact gtgctttctc
      121 gagttttgct ttaatttcat caaccacagc ctgtttttcc gtcaaatgtt cttttgatgc
      181 cattagtatt caactcgaac ctaaaatctt agtataatgg agatgatcga attaagcatt
      241 gacaacaaag tgttgagagt aaaaaaaggt acaaccattc tcaaagctgc ccgcaaagtg
      301 ggaatcgaaa tccccactct ttgtcatatg gagcttcaca acatgaatat agaaaacaaa
      361 ccgggcagtt gccgggtatg tgtggttgaa gtagacgggc gtcgtaacct ggcccccgcc
      421 tgcatcaccg aagtgacacc cggcatggta gtcaataccc atacagtcag ggtgctgaat
      481 gcccgtaaaa ccattgtgga attattaatt tcagaccacc cctacgattg tctggtttgc
      541 gccaaatcgg gaaattgcga tttgcagtcg atcgctcata aattcggtat ccgccgcgta
      601 cactttaaag gcgaaaaatc gacctaccgg aaagatactt cgccttctat aatacgtgat
      661 gtcgacaaat gcattatgtg ccggcgttgc gaaatgatgt gcaacgaggt gcaaacagtg
      721 ggagccttat ctgccgttaa cagaggtttt atctctgtag tggcgccggc tttcgaaatg
      781 aacctggaac aatcgtcctg tacctattgt ggacaatgcg tttctgtttg tccgacaggt
      841 gcgctgaccg aagtagacag cacaaataaa gtattactgg ctctgatgga caagagcaaa
      901 accgtaattg ttcaaacggc accctctgtc agggctgctc tcggcgaaga attcgggctg
      961 cctgccggca ccttggtaac cggcaaaatg gtggctgctt tgcgcagaat aggctttaaa
     1021 tacgtttttg acaccgattt tgccgctgat cttactataa tggaagaagg caccgaactg
     1081 ctgggcagac tcaaagctca tttagccgga gaaagcaatg ccaaattacc tattctaacc
     1141 tcctgctgcc cgggctgggt caatctcatg gaacacaatt tccccgattt gctacatatt
     1201 ccttcaacgg caaaatcgcc ccaacaaatg ttcggagcca tcgcaaaaac atactttgcc
     1261 gacttaatcg gcgtgaagcg ccaagacttg gtggttgttt cggtcatgcc ctgtttggcc
     1321 aaaaaatacg aatgtcagcg ggacgaattc aaggttgatg gcaatcccga tgtggatttc
     1381 tctataagta cccgggaatt ggccgacctt attaagcaat ccaacatcga cttcctgaat
     1441 ttgcccgacg aggactttga tactcctctg ggagaatcga ccggtgccag cgtaattttt
     1501 ggaaatacag gcggagtcat cgatgcagcc tgtcgtacag cttacgaatt gtacaccggc
     1561 aaaaaactcg atgtcattga atttgaaagc ctcagaggaa aagaaggcat acgttccgct
     1621 aaactggatt ttgacggcct aacgttgaat atcggcattg ctcatggttt gtctaatgcc
     1681 cgaaaattgc tggacgatgt cagagcaggt aaatccgatt atcacgccat agaaatcatg
     1741 gcttgtgtcg gtgggtgcat tgcgggtgga ggccaaccct atcaccacgg ggacagcaaa
     1801 attctcagac aacgaagaaa tgccatctac agagaagaca aaaacaaaac tatacggaaa
     1861 tcgcacgaaa atccttacat cattcaactt tataaggatt ttctgggtca accctgcgga
     1921 caaaaatcgc acgaattgct gcacaccgtt tattacgaca aaagcaggga agagattacc
     1981 atccatcacg actaatagtt atcaaaaaaa aataaaaatc aaacaccatg gttcctaaat
     2041 taatatgcga cgacgagatg gcagatgttc atgcacctgc taccagcgat aaagacagct
     2101 taaaggtaac catcaaactg tccgtcgaga agataaatgc catcaaccgg atttgtgcct
     2161 cctatgacaa cgatccggaa gaattgatca gtgtcctgca cgaaacacaa aacactttgg
     2221 gttatctgcc tgccgaagta caggaaatca ttgcgcacaa cttgaagata tcggctgccc
     2281 acgtatacgg tgttgttaca ttctactcct attttaccat ggttccaaag ggcgatcacc
     2341 ccatttcggt ctgtatgggt actgcctgtt atgtcagggg agcagaaaaa atattggatg
     2401 aactcaaaaa ggaactcaac ataaacatag gtgagaccac acccgaccaa cgcttttcta
     2461 tcagttgctt gcgttgcgtg ggggcttgcg gcctggcacc tgtaatgatg gttggagaaa
     2521 aagtgtatgg cagggtttcg ccaaacgatg tcagaaagat tctcgaagaa tatcgtgagg
     2581 acaattgcga agaatgtaaa ccaactcagc aattgtaagc tgtagtttta tttgtcctac
     2641 atcaggcctt cgtttttaag ttcacgcaaa accaattcgg tcatttcgct tttgaataaa
     2701 aattcgtaac ctacattcat cacataggcg ttaagacgca tcttaagtac cggttcttta
     2761 ttagtctttt cgctgaaaaa aataactgca atcggcttat ttaagtagac gtatttggaa
     2821 atttgggcta cttcgatggc aatttgcctt accctttgcg tatcaatcaa atgaggaaga
     2881 taaatatcgg ccgacaccag acaattattt aggccggagt tggtgttgga gatggattga
     2941 ttcattattt cagagttagg aacggaaaca atattatcgc ccggcgtaac aatacgggtg
     3001 gaacgaatcc ctatttccac tacttcacca taatatttac ctgtttctat tctatcgcct
     3061 atctgaaaag gtttatcaaa aagtatcatc agaccgccaa aaaggttttt gagtaaatcc
     3121 tgagaggcaa aaccaatggc tactcctatc gaagcaaaaa aggccaacac ggtttctttt
     3181 ggaggactga agataccgct tacaataatg taaaccacca tactccaaac aagaattttt
     3241 atcatcggta agactctgcg aaccaacgat ttatgacgag gcttgcgttc ggcatataag
     3301 ttcagaattc cggttagtat ttttaaggaa aagtaaccca agagcaagaa aacaaccgac
     3361 cagaaaatct ttggaaaaga tatcaatcca cttacatctg gggcctgagt taactcctga
     3421 gccaaattac cgcgctctgc cacaggcggg agagctgtct ggcttgccag cgtgtcggtt
     3481 attagctgtg aggaatcggt agtcgttatg gtgtttgctg cttgtccatg cagcaagctg
     3541 ctgaagctta tgcaaattaa aaacaaaagg ctgtatccta ttattttcct ggttttcatc
     3601 tcgttgaaat tatctataca ttttaataaa tcaggttgtt atccctaagc atttgtatgg
     3661 tagaacggta aagaaggggg tgaattttgt aaaatccgtc ctcatccaaa actattccac
     3721 ggtcgtttaa tcgcattaac agtaaagcag cctgccgttg cgggcaattc aacacggcgg
     3781 ccagttcctc cggattcagg ctttcgtgca acaataaagc atgtaaagca aaaacagcct
     3841 cccggggctg gttatacaaa aaactaaaat cgagcttatc cagcgattca aggtaaaccc
     3901 gattattatc tatgctatgt atactggcaa gccagagcaa aagagcgaac gaaatgttgg
     3961 attccgtgat ctgattaagc atggagaaat acttggtttc gagtacttcc tgttgtttgg
     4021 attcctccat ttttgtaaat tttttatcgg ccagatcacg attggagggc atataactca
     4081 aatcgtaacc actggctcta tgtctgagaa gaataatttt aattattttc tccagctcta
     4141 aagcttgcat ttgaattaat accggaaaat aaccgaacaa gcctatggtt ttgttcaagt
     4201 agtctgctga atattgcccg caggaacaaa tccaaaacac cttttcgctg cttgtgctga
     4261 taat
//
LOCUS       DAOSGL010000199         4261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_18734_length_4261_cov_4.662863, whole genome shotgun sequence.
ACCESSION   DAOSGL010000199 DAOSGL010000000
VERSION     DAOSGL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18734_length_4261_cov_4.662863"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKY68_06650"
     CDS             complement(<1..332)
                     /locus_tag="PKY68_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24750.1"
                     /translation="MNLAAVILLLLSAKMSVLSPERSVLSSYPNYAFLPLAFINLFFV
                     LYWALRKSVWFLMSLLPLLLCLPEQKAWFPVKLIKEQKKEDKTIKVLTYNTMQFSSYR
                     SHIKQDPNP"
     gene            complement(438..1337)
                     /locus_tag="PKY68_06655"
     CDS             complement(438..1337)
                     /locus_tag="PKY68_06655"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOH24751.1"
                     /translation="MASIWDNIKFRYLQGNIATKLIFINVGIFLLIKLFVVFCRLFQF
                     DGSRFILLLQLPASWDQILLRPWTLLTYMFVHLGFFHLLFNMIWLYVFGLYFLRWFSS
                     RQLLMVYLLGGLTGGLFYIIAYNLFPLYSSSVEHTVLMGASASVLALTLSVAFYRPNE
                     SLVFMFIGQVKLKWLAVAMVVIDLLSLAGDNAGGSLAHLGGALFGLLFGFFSRENLSL
                     SIFGSKTGGSRGFRAKTGKRTKYHRPRFEKANRTRPESDQEYRDRKKNEEDYRDAILD
                     KIKQSGYESLSKKEKQFLFKSGQ"
     gene            complement(1318..2088)
                     /locus_tag="PKY68_06660"
     CDS             complement(1318..2088)
                     /locus_tag="PKY68_06660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOH24752.1"
                     /translation="MSVYTPRNSFWSSIPPVTKNLIIINFLLWLAAMLFPGIQDFLGL
                     HYFKSDKFNLLQPVTYMFMHSTRGIGHVFFNMFAVFMFGRLLEQVWGPKRFLLFYLVT
                     GVGAGIIQQITWYIDLIPFMQAVDNYIITGEVGILGKYWNIDPSIPYSAEMVLRSRDA
                     LLDAMLTIGASGAVFGILLGFGMLFPNAPLYIMFIPVPIKAKYMVIGYGVIELFAGVA
                     NFSGDNVAHFAHLGGMIFGYFMIRFWKKKGIGHGIYMG"
     gene            2321..2590
                     /locus_tag="PKY68_06665"
     CDS             2321..2590
                     /locus_tag="PKY68_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOH24753.1"
                     /translation="MNKTELISAIAEKAGLSKVSAKKALEAFLSSVEETLAKGEKLTL
                     VGFGTFSVVKKAARTGANPATKQPIKIPAKNVVKFKAGASLAAKV"
     gene            2677..>4261
                     /gene="argS"
                     /locus_tag="PKY68_06670"
     CDS             2677..>4261
                     /gene="argS"
                     /locus_tag="PKY68_06670"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOH24754.1"
                     /translation="MNIYTLLGDKTRQCLQALYQLQADDSLLQLQKTKKEFEGDLTLV
                     VFPFIKAAKKSPEATAEEIGSWLLQHTPELSGYNVIKGFLNLSFSASYWLNTLQDIAG
                     DTDFGLFEAGESAPLVMIEYSSPNTNKPLHLGHIRNNLLGFSLSRIVAANGCRVVKTN
                     IVNDRGIHICKSMLAWQKWGKGETPESSGKKGDHLVGDYYVLFDKNYKQELAELQKQG
                     LSLEEAENTSLLMQEAREMLRAWEAGDSEIRSLWETMNNWVYLGFDESYAKLGVDFDK
                     IYYESQTYLSGKEKVLEGLDKGIFYRNEDGSVWADLSSEGLDRKLLLRADGTSVYMTQ
                     DIGTAALRFEEYPIDKMIYVVGNEQNYHFKVLSILLDKLGFAWGKDLVHFSYGMVELP
                     EGKMKSREGTVVDADDLIEEMISTARETSEELGKLDSCSPEEAADIARIVGLGALKYF
                     ILKVDPRKNMTFNPKESIDFNGNTGPFIQYTYARIRSVLRKAEELGYRAQVQEQLADC
                     LADKELSLIKNLYEFPVVVK"
BASE COUNT         1315 a          936 c          935 g         1075 t
ORIGIN      
        1 ggattggggt cttgcttgat atgagaacga taactagaaa attgcatcgt attataggtc
       61 aacactttaa ttgttttatc ctcttttttc tgctctttta tgagtttgac cgggaaccag
      121 gctttctgtt ccggcaaaca gagcaaaagg ggtaataaag acattaaaaa ccaaacactt
      181 ttgcgaagcg cccaatagag aacaaaaaaa agatttataa aagcgagggg caaaaaagca
      241 taattcggat aagaactcag cacggaacgt tccgggctca aaacggacat tttggcacta
      301 agcaacagca aaatcacagc tgccagattc atcagaatca atactgaatc tcttaacacc
      361 cgaagtatat tcggtctttt gggggcgttt ttcattgagc catattcaag gctgcggcgg
      421 caagccgggg ttattaatta ttgtccggat ttgaacaaaa attgtttttc ttttttgctc
      481 aggctttcat aacctgattg cttgatttta tccaggatag catccctgta atcttcttcg
      541 ttttttttgc gatcccggta ttcctgatcg ctttcaggcc tggtcctgtt tgctttttca
      601 aagcgtggac gatggtattt ggtccttttg ccggttttgg cccgaaaacc gcgggaaccc
      661 ccggtttttg agccaaaaat actcaaactt aaattttctc ttgagaaaaa tccaaaaagt
      721 aaaccgaaca aggctccgcc taaatgagcc agactgccgc cggcattatc tccggccagg
      781 ctcagcaaat caattacaac catggccacg gccagccatt taagcttaac ctggcctatg
      841 aacataaaca ccaggctttc atttggccgg taaaaggcta cggacaaagt caaggccagt
      901 accgaagccg aagcacccat caacacggtg tgttctaccg aagaactgta gaggggaaaa
      961 agattgtagg caattatgta aaacaaacct cctgttaagc cacccaacaa ataaaccatc
     1021 aggagctgac ggctactgaa ccaacgtaag aagtacaggc cgaatacata cagccagatc
     1081 atgttgaaca acagatggaa aaaccccaga tgcacaaaca tgtaagtcaa aagagtccag
     1141 ggtctcaaca agatttgatc ccaggaagct gggagttgca gcaagagtat aaatcggctt
     1201 ccgtcgaatt gaaacagacg acagaaaacc acaaacagct tgatgagcaa aaatatcccc
     1261 acattgataa atatcagttt tgtagcgata ttcccctgta gatatctgaa tttaatgtta
     1321 tcccatatag atgccatggc ctattccctt ttttttccag aaacggatca tgaagtaccc
     1381 gaaaatcatt cctccgagat gagcaaagtg agccacatta tctccgctaa aattggccac
     1441 cccggcaaaa agttcaatca caccgtaacc gattaccata tatttcgctt tgatgggtac
     1501 cggaataaac ataatgtaaa gaggtgcgtt ggggaacaac ataccaaaac ccagtaaaat
     1561 accaaaaacg gctccggaag ctccgatggt gagcatggca tccaggagag catctcggga
     1621 acgtaaaacc atttcggccg aataaggtat cgaaggatcg atgttccagt atttccccaa
     1681 aataccgact tctccggtta ttatataatt gtccaccgcc tgcataaaag gaattaaatc
     1741 aatataccag gtgatttgct gaataattcc cgctcctacg ccggttacca ggtagaataa
     1801 caaaaaacgc ttcggccccc atacttgctc gagcaaacgc ccaaacataa aaacggcaaa
     1861 catattgaag aatacgtgac cgattcctct ggtactatgc ataaacatat aagtgaccgg
     1921 ctgaagcaaa ttgaacttat cggacttaaa gtaatgcaag cccaaaaaat cctgaatgcc
     1981 agggaaaagc atggcggcca gccatagtaa aaaatttatg attatcagat ttttggtgac
     2041 cggagggata gaagaccaaa aagaatttct gggagtatat accgacataa aaaaaatatt
     2101 tgcgcaaagg taaggatttt tccaaaatcc acttatcttt gtttctctaa acagctgtat
     2161 tcatcgaaat tctgagaaaa aagcgtcccg gaagtgtttt ttcttgtgaa ttacgttttt
     2221 ttacgtgttt tatttgcaaa acttaaaatt ttcattttta tttgcgttca agttttggat
     2281 ccctccttat ttttcatttt tatttattat cctaattatc atgaacaaaa cagaacttat
     2341 cagtgctatt gccgaaaaag ccggcttaag caaagtgagt gcaaaaaaag ctttggaagc
     2401 atttttgagt agtgtagagg aaaccttggc aaaaggtgaa aaactgactt tggttggttt
     2461 tggtactttc tcagtggtaa aaaaagctgc ccgtactggt gcgaaccctg ccaccaagca
     2521 acctatcaaa atccccgcca aaaatgtcgt aaaattcaaa gccggtgcaa gtttggctgc
     2581 caaagtatga agctaagctt cttatacgaa aaaaaccgct tggatttcca ggcggttttt
     2641 ttaattttgc aacttgattt ttcttgcccg attacaatga acatatacac cctgctcggg
     2701 gataaaaccc gccaatgcct gcaggcttta taccaattgc aggctgacga ttcgctgctt
     2761 cagttgcaaa aaacaaaaaa agagtttgag ggtgacctga ccctggtcgt cttccctttt
     2821 ataaaggctg ccaaaaaatc gccggaagct acggcggagg aaatcggtag ttggttactg
     2881 caacataccc cggagttaag cggttataac gtaatcaaag gttttctgaa tttaagtttc
     2941 tctgcttcgt actggctgaa tactctgcag gatatagccg gcgatactga ttttgggcta
     3001 ttcgaagccg gcgagtcggc tccattggtg atgatagaat actcttcgcc gaataccaac
     3061 aaacctctgc atttaggaca tataagaaac aacttgctgg gattcagcct cagtcgcatc
     3121 gttgcagcca acggctgccg ggtagtgaag accaacatag taaacgacag ggggatacat
     3181 atctgtaaat ccatgctggc ctggcaaaaa tggggcaagg gcgaaacgcc cgaatctagt
     3241 ggaaaaaaag gagatcatct ggtgggcgat tattatgtgt tgtttgataa aaactataaa
     3301 caagagctgg cagagctcca aaaacaaggg ctgagccttg aagaagccga aaacacttcg
     3361 ctcctgatgc aggaagcccg cgaaatgctg cgggcatggg aagccggcga cagcgaaatt
     3421 cgcagcctgt gggaaaccat gaacaattgg gtttacctgg gttttgatga aagttacgct
     3481 aaactgggcg tggacttcga caaaatttat tacgaatcgc aaacctatct ttcgggcaaa
     3541 gaaaaagtac tcgaaggcct cgacaaaggc atcttttacc gcaacgagga cggctcggtc
     3601 tgggcagacc tcagctccga aggcctggat cgaaaattac tcctgcgcgc cgacggcacc
     3661 tcggtttata tgactcagga cataggcacg gctgctctgc gcttcgaaga ataccccatc
     3721 gacaaaatga tttacgtggt ggggaatgag caaaattatc atttcaaagt tttatccatc
     3781 ctgctcgaca aattaggttt tgcctggggc aaggatttgg tgcatttttc ttacggcatg
     3841 gtggagctgc ccgaaggcaa aatgaaatcg cgcgagggta cggtagttga cgccgacgac
     3901 ctgatagaag aaatgatttc aactgcccga gaaacttcgg aagaactggg caagctcgat
     3961 tcctgcagtc cggaggaagc tgccgatatt gcccgtatcg tgggcctggg agctttgaaa
     4021 tactttattc tcaaggtcga tccccgcaaa aacatgacct ttaatcccaa agaatccatc
     4081 gatttcaatg ggaacaccgg accctttatc caatacactt acgcccgtat acgttccgtt
     4141 ttgagaaaag cggaggaatt gggataccgg gcacaagtac aggaacaact ggccgactgc
     4201 ttagcggaca aggaattatc ccttatcaag aacctgtatg agtttccggt agtagtaaaa
     4261 g
//
LOCUS       DAOSGL010000200         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_18763_length_4256_cov_5.421566, whole genome shotgun sequence.
ACCESSION   DAOSGL010000200 DAOSGL010000000
VERSION     DAOSGL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18763_length_4256_cov_5.421566"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="PKY68_06675"
     CDS             complement(<1..1497)
                     /locus_tag="PKY68_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HOH24755.1"
                     /translation="MTYLKFDKALMVNLQQSLTKELLQTNKSGAYSYSTIVDCNTRKY
                     HGLLVIPVPELDGKNHVLISSLDTTVIQHGAEFNLGLHKYEGDNYSPQGHKYIREFEC
                     DSNPSTIYRVGGVILSKEKIFVSHENRMLIKYTLLEAHSPTKLRLRPFLAFRSINDLC
                     KENNYVNKEYQLVDNGIKLCLYNGYPELHMQLSAENEFHYSPDWYRNIEYLKEQERGY
                     DFKEDLFVPGYFELPIKKDESIYFSAGISVIQPATMQKMFEEEKSIRTPRNSFTNSLK
                     NAAYQFYNKHPDGQRYLLAGYPWFECRARDMFVSLPGCTLAAEDPGLFDAIMETGEKG
                     LRNFMEHKPIDCAITEIDAPDVLLWMIWALQQYAKDQGTEKCAGKYNKLLFDALDFII
                     GQKHDKLILHNNGLLFTNGKEQPVSWMNSMMYGKPLVPRTGYLVEFNALWYNALCFAS
                     EMAALAGKAKLQAKYSELAQKAGVSFVEVFRNDFGYLYDFVDGQTIDWS"
     gene            1767..2759
                     /locus_tag="PKY68_06680"
     CDS             1767..2759
                     /locus_tag="PKY68_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24756.1"
                     /translation="MEKRQKELNLSELLMLLFKGIGNLIKWLWNLFIQALRLSYRKKW
                     IILGFIVAGLVWGYFASTPDKQIHKASITLKTVIKGSPYSKDFIKTLNADCGKKDKSS
                     LAKKLNISEEEAEKIVSLKSFYLVDIGADGILDQVDYKESFKGDTINVWSNNFLYVEI
                     LSKDEKLFPTIREGIVYALNNDSSVQKEKAFMNLEHSDWVSMLDEELEKVKELQSKMF
                     AEDVSNVTSLSLRQKPKQQWSDPNVMTVLGETKMQMFAGDKIHSMVIMNNHKRAIKEL
                     NEGAIRVELPILYLGVTNPRYKVMITRAFIAYLLGLGLCAYLENRKKIIAFLEA"
     gene            complement(2850..>4256)
                     /locus_tag="PKY68_06685"
     CDS             complement(2850..>4256)
                     /locus_tag="PKY68_06685"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOH24757.1"
                     /translation="VFSANGIYVYLFDDLRPTPEVSFAIRHLKCQSGVILTASHNPKE
                     YNGYKAYWDDGAQVVPPHDHNIINEVQAIKDISSVRFEGNKELIKLIGAEIDEKYIKQ
                     IKTISLSPEAIRKHEDLKIVYTPIHGTGVRIIPACLRAFGFKNIINVPEQDVVSGDFP
                     TVVSPNPEEPAALSLALKRAAEVDADIVMASDPDSDRLGIAIKNDREEWMLVNGHQTA
                     LIFIYYLIKRRSELGMLESGKEFIVKTIVTTELIKEIADRHKVDMFDTYTGFKWIAAI
                     IREFEGKRQYIGGGEESYGFLPQDFVRDKDAVSSCALMAEILAWAKEQGKSLYELLLD
                     IYMEYGFSREKGISVVKKGKSGAEQIKSMMDNFRSKPVNELAGSPVILVKDYMSLKAY
                     KPESGESFDLKMPETSNVLQYFSLDGSKVSIRPSGTEPKIKFYIEVRDVLKSHSEYPA
                     KCKLAEEKFKKIMKSLGL"
BASE COUNT         1216 a          923 c          874 g         1243 t
ORIGIN      
        1 actccaatct atcgtctgcc cgtccacaaa atcgtacaaa taaccgaaat cgttgcggaa
       61 tacttctaca aaagagactc cggccttctg tgcaagttcc gagtatttgg cttgtaattt
      121 cgccttgccg gccagggcgg ccatttctga ggcaaagcaa agggcattat accacaaagc
      181 gttgaattca accaaataac cggttctcgg caccagaggt ttgccgtaca tcatggaatt
      241 catccagctg acaggttgct ctttgccgtt ggtaaagagc aatccgttgt tgtgcaaaat
      301 aagcttatcg tgcttttgac cgattataaa atccagtgca tcaaacagca atttattgta
      361 ttttccggcg catttttccg taccctgatc tttggcgtat tgttgaagag cccatatcat
      421 ccacaacaag acgtccggcg catctatttc cgtaattgca caatcgattg gtttgtgttc
      481 catgaaattt cgcaatcctt tttcgccggt ttccattatg gcatcaaata aaccgggatc
      541 ttcggccgcg agagtacaac cgggaagaga gacaaacata tcccttgccc ggcactcaaa
      601 ccaggggtaa cctgccaaca ggtaacgctg tccgtcggga tgtttgttgt aaaactggta
      661 agccgcgttt ttcaggctgt tggtaaaact gtttctgggt gtacgaattg acttttcttc
      721 ctcaaacatc ttctgcatag ttgcaggctg gataactgaa atacccgccg aaaaatatat
      781 gctttcgtct tttttgatgg gtaattcaaa ataacccgga acaaacaagt cctctttgaa
      841 atcatagcct ctttcctgct ctttcaaata ttctatattc ctgtaccagt cgggagaata
      901 atgaaattcg ttttccgcgg ataattgcat atgcaactcg gggtaaccat tgtaaagaca
      961 aagcttaatc ccgttatcta caagctgata ttctttattt acataattgt tttccttaca
     1021 caggtcatta atgctgcgaa aagccaaaaa aggtctcagc ctgagtttgg tgggagaatg
     1081 cgcttcgagt aaggtgtatt ttatcagcat gcggttctcg tgcgaaacaa atattttttc
     1141 cttggaaaga attacacctc ctacccggta aatagtcgaa ggattggaat cgcattcaaa
     1201 ctcgcgaatg tatttatgtc cctgagggct gtaattgtct ccttcgtatt tgtgcaaacc
     1261 aaggttgaat tctgctccgt gttgaatgac ggtcgtatca agagaagaaa tcagcacgtg
     1321 atttttcccg tccagctcag gaacgggaat caccaacaaa ccatgatatt tcctggtatt
     1381 acagtcgact atggtcgaat agctgtaggc tcccgatttg ttggtttgca gcaactcttt
     1441 ggtcaacgat tgctgcaagt tgaccattaa ggctttgtca aatttcaaat aggtcataac
     1501 agtacatgtt ttgcttattg cgattaataa cctgcatcag taacaaatcc ggtacttatg
     1561 caaaaaacaa tagacgaaga taggtttttt tggggaaatt acaagcgata agatcctaaa
     1621 taaagggcaa caaatatatg ttttacatca tcttttggcc tgtttttggc cggtttgctc
     1681 tttatggcgg ggattttgtt gacaattgca gaattttagt acttttgcat tctcatttta
     1741 tacatttact cacgacactc cgtactatgg aaaaacgaca aaaagaactc aatctttccg
     1801 aactcctaat gcttttgttc aaaggtatcg gcaatttaat caagtggctg tggaatcttt
     1861 ttatacaagc tttgcgcttg agctaccgga aaaaatggat tattctggga tttattgtgg
     1921 ccggacttgt atggggatat tttgcttcca cacccgataa acagatacac aaagcgtcca
     1981 tcacgctaaa aaccgtcatt aaagggagtc catacagcaa agattttatt aaaacattga
     2041 atgccgattg cgggaaaaaa gacaagagca gcctggcaaa aaagctgaat atctccgaag
     2101 aagaagccga aaaaattgtc tccctgaaat ctttctattt agttgacatt ggtgctgatg
     2161 gtatcctcga ccaggttgat tataaagaaa gttttaaagg tgacacgatc aacgtctgga
     2221 gtaataattt cttatacgtt gaaattttat ccaaagatga aaaattattc cccacaataa
     2281 gagagggcat tgtttatgcc ttgaataatg actcatctgt gcaaaaagag aaagccttca
     2341 tgaatctgga acattccgac tgggtttcga tgcttgatga agaattggaa aaggttaagg
     2401 aattacaatc aaaaatgttt gctgaagacg taagtaacgt cacttctctg agtttaagac
     2461 aaaagcccaa gcaacaatgg tccgacccca acgtaatgac tgtgttgggc gaaacaaaaa
     2521 tgcaaatgtt tgccggtgat aaaattcatt ctatggtaat tatgaacaat cataaacgcg
     2581 cgattaaaga acttaatgaa ggtgccatac gcgtagaatt acctatttta tacctgggag
     2641 tgacaaatcc ccggtacaaa gtgatgataa cacgagcttt catagcttat cttttgggcc
     2701 tgggactctg cgcttacctg gaaaaccgta aaaaaatcat tgcttttttg gaagcataac
     2761 aatcatccgg cgtcaggatc gtttcaccgt caaccccaaa gaaccgggga agttgtatgg
     2821 tcttgttaga aacctgctga aaaaaggtac tacagaccca aagatttcat tatcttctta
     2881 aatttttctt cggccaactt gcatttggcg ggatattcag agtgcgattt caacacatcg
     2941 cgtacttcga tgtaaaattt aatctttggt tccgtaccag aaggacggat agacaccttt
     3001 gatccgtcca ggctgaagta ttgtaacaca ttagaagttt ccggcatttt aagatcaaaa
     3061 ctttctccgc tttcaggctt ataagctttc aggctcatat aatccttaac caaaattacc
     3121 ggagaacccg ccagttcatt aaccggcttg gatctgaaat tgtccatcat ggacttgatt
     3181 tgttcagccc ccgatttccc ttttttaacg acagaaatgc ctttctctct cgaaaaaccg
     3241 tattccatgt aaatatccag caacaattca tacaagcttt tcccttgttc tttggcccaa
     3301 gcaagtattt cggccatcaa agcacaggac gaaacagcgt ctttgtcgcg aacaaaatcc
     3361 tgaggcagga agccataact ttcctcacct ccgcctatgt attgtcgttt accttcgaat
     3421 tcccggatta tagcggctat ccatttaaag ccggtatacg tgtcgaacat atccacctta
     3481 tgcctgtcgg caatttcttt gatcaattcg gtagtaacta tggttttgac gataaactcc
     3541 ttgccgcttt cgagcatccc caactcggaa cgtcttttaa tcaggtagta gataaagata
     3601 agggcggtct gatgtccgtt gaccagcatc cattcttccc ggtcgttttt aatcgctatt
     3661 ccgagacggt cggaatccgg atcggaagcc attacaatat cggcatctac ttcggccgct
     3721 cttttcagcg ccaacgacaa agctgccggt tcttcgggat tgggcgaaac aaccgtagga
     3781 aagtccccgc tgaccacgtc ctgttcgggc acattgatga tgtttttgaa gccaaaagcc
     3841 cggagacaag caggaataat tctgacaccg gtaccgtgaa taggcgtata tactattttc
     3901 aggtcttcgt gttttcgaat ggcctcgggc gatagagaaa tggtcttgat ctgtttaatg
     3961 tatttctcat cgatctcggc gccaatcaac ttaatgagtt ccttatttcc ctcgaagcgg
     4021 acagagctga tatccttgat agcctgtact tcgttaatga tattgtgatc gtgaggaggc
     4081 accacctgag ccccgtcgtc ccaataagct ttataaccgt tgtattcctt aggattgtgc
     4141 gaagccgtca atataactcc gctctggcat tttaaatgac gtatggcaaa agacacttcg
     4201 ggggtgggac gtaaatcgtc gaataaatac acataaatgc cgttagctga aaatac
//
LOCUS       DAOSGL010000201         4217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_19013_length_4217_cov_3.407496, whole genome shotgun sequence.
ACCESSION   DAOSGL010000201 DAOSGL010000000
VERSION     DAOSGL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19013_length_4217_cov_3.407496"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2158
                     /locus_tag="PKY68_06690"
     CDS             <1..2158
                     /locus_tag="PKY68_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24758.1"
                     /translation="NCLRRTTTMVPDDTIPFRTDLPDNPQFARFKESGMYLKTPGNWT
                     MVHFTQPYFDKKNMNNEDFDRLYPGTQRQNGDIHIFGAKIIEGNIVNLNGYIHVLDKV
                     IMPPLSMWEYMTANENLSEFCRLLERFCEPVYDPAATEKLHEMQPESTDSVFYISFFD
                     TADRTIVDDKGNEYTVESLLFSPAANETQNQAIVGQPPSDMPAIFAPDNQAMQHYIEN
                     SFLSEYPLWDSVPDDLAAKFVRTHMKRSFLNALPTRINDIVDDVKGDHMGYTLENVTD
                     AQICRNGLVYTLNDVVDPRDFKTVVAPLLKNPETRIFNWIINESSNNTALKFNYYLTS
                     LENNFTLFIPLDSSFSAYPDPVNQTTATLEKKKMRFFFDEDKKHVNYISLNNAGDSIA
                     APTYNADVVRNRLKDIVDHHIVVGERSPGQKYLQTKGGAYIKVDESAQGLRFQGAGDL
                     EKGSYANVIAKYDYNNGIHNGTVFLIDKTLEHTLKSFYSLAQSNITADSNISEFFKLL
                     EDFDDPDATFFADREPATDKTLFYRNVSAAKQTEYVRPIFYKSDGIDYFVPFLGLYNY
                     TVFVPINEALNRACTEDRLFMNKDQIASLGNDSAMIAEIKRTITFIKNHFVDGAYFTE
                     QYYMDEWAEDDGLPRTTTTSAKNQKNRQFYNLYITQNGSHLSVKKNAAGSIMGTTLSG
                     SENIMCRQYSFAGGNIATNSRIVVHAIDGVLSPNNE"
     gene            2168..>4217
                     /locus_tag="PKY68_06695"
     CDS             2168..>4217
                     /locus_tag="PKY68_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24759.1"
                     /translation="MKRIKITVVCLLCLSWTLTAYAQQIIVRGLVTSEDHEPLISVTI
                     AEIDRNNRIVNGTVSNYDGEYVLQVRDKANKLRFNYVGFKAQDQEIGERSVINVTMQE
                     ESLELAEAQVTGKAVTNTGTMSIPTREISFAMQKVDAKEFEGIQVTSVEDALQGRVSG
                     LDIVSNSGAPGSGMSMRIRGTTSLRSDGSQPLIVVNGVPFETTVASDFDFATAREEQY
                     AELLTVNPDDILEIVVLKDAASTAIWGAQGANGVLQITTKKGARGKPKVQYTYRLTSA
                     FMPDPIPLLNGDEYTMLMKEEYLNPTLNTSQGNIPEYGYVQDGSAFFWNYNNNTDWVK
                     EITRAAWKHDNNLSISGGGERGDYRISFGYLDEEGIVYGQGLRRFTNRTDLGYVLSDR
                     LIFRTELAYTFSDNDRNYDGDILNKALRKMPNVPVYTRDEKGNYTDVYFNIDRASSNL
                     HADQRDMVNPVGLVNLASYNSKSYRIMPTFRLQYDILDPGKGLLRFKSSVSMDVNSIH
                     TNRFFPKELSGANYDDTSINYAFDGDEESINIQLDNNLTWQPRLSNGHAFMVYGAVQT
                     SINKGSSQSLGSYWAPSSYIQNVSEGFTSETKSYSSESHGYGMFVSSHYAYLERYIVD
                     FVVRRDVSSRFGKNNRVGYFPGISLKWIASDENFLQDVSWINMLAFRPSWGISGNTPK
                     S"
BASE COUNT         1177 a         1164 c          944 g          932 t
ORIGIN      
        1 caactgtctg aggcgtacca ccaccatggt accggacgac accatccctt ttagaaccga
       61 cctgccggac aatccgcaat ttgcccgttt taaagaaagc ggcatgtacc tcaaaacccc
      121 cggcaactgg accatggtgc actttaccca gccctatttc gacaagaaaa atatgaacaa
      181 cgaggacttt gatcgtcttt atccgggcac tcaacgccaa aacggagaca ttcatatttt
      241 cggagccaaa atcatcgagg gcaatatcgt gaacctgaac ggctacatcc atgtgctgga
      301 caaagtgata atgccgccat tgagcatgtg ggaatatatg acagccaacg aaaacctctc
      361 tgaattttgc cgtttgctgg aacgcttctg tgaacccgtc tacgatccgg cggctaccga
      421 aaaactacac gaaatgcagc ccgaaagtac ggactcggtc ttctacatca gttttttcga
      481 cacggccgac cgtaccatag tcgacgacaa aggcaacgaa tatacggtag aaagcctgct
      541 cttttcgccg gccgccaacg aaacccaaaa tcaggctatc gtggggcaac cgccttcgga
      601 tatgccggcc atttttgctc ccgacaatca ggccatgcaa cattacatcg aaaacagttt
      661 ccttagcgaa tatccgcttt gggatagtgt tcccgacgac ctggccgcca aattcgtaag
      721 gacgcacatg aagcgttcct ttttgaatgc cctgcccacc cggataaacg acatcgtgga
      781 cgacgtcaag ggcgaccaca tgggctacac gctcgaaaat gtgacagacg cccaaatctg
      841 ccgcaacggc ctggtttaca ctctgaacga tgtggtcgat ccccgcgact tcaaaacggt
      901 ggtagctccc ctgctcaaaa atcccgaaac ccgcatattc aactggatca tcaacgaaag
      961 cagcaacaat acggctctga agtttaatta ctacctgact tccctcgaaa acaacttcac
     1021 cttgtttata cctttggatt ccagcttctc ggcctacccc gatccggtga accaaaccac
     1081 tgccaccctc gaaaaaaaga aaatgcgttt cttcttcgac gaagataaaa agcatgtcaa
     1141 ttacatttct ctcaacaacg ccggcgactc cattgccgcc cccacataca acgccgacgt
     1201 tgttcggaac aggctcaaag acatagtaga ccaccatatt gtggtaggtg aacgctcccc
     1261 gggacaaaaa tacctgcaga ccaagggagg agcctatatc aaagtggacg aaagcgccca
     1321 gggtttgcgc tttcagggag ccggcgatct ggaaaaaggc agctatgcca acgttattgc
     1381 aaagtacgac tacaacaacg gcatccacaa cggcacagtc tttttgatag acaaaacctt
     1441 ggaacatacc ctgaaatctt tctactccct ggcacagagc aatatcacag ccgatagcaa
     1501 catcagcgaa ttctttaaac tgcttgaaga cttcgacgat cccgatgcca ctttcttcgc
     1561 cgatcgcgaa ccggccaccg acaagaccct gttctaccga aatgtttcgg cagcaaaaca
     1621 aaccgagtac gtgaggccta tcttctacaa aagcgacggc atcgattatt ttgttccctt
     1681 cctgggcttg tacaactaca cggtctttgt acccatcaat gaagccctga acagggcttg
     1741 taccgaagac cggctattta tgaacaaaga tcagattgcc tctctgggca acgattcggc
     1801 catgatcgcc gaaatcaaaa gaaccatcac attcattaaa aatcattttg ttgacggagc
     1861 ctattttacc gaacaatatt atatggacga atgggccgaa gacgacggac tgccccgtac
     1921 tactaccact tcggccaaaa accagaaaaa caggcaattc tataatttgt acattaccca
     1981 gaacggttcc catctgagtg tcaagaaaaa cgccgccggc tccattatgg gtactaccct
     2041 tagcggcagc gaaaacatca tgtgccggca atacagcttt gccggcggta acatcgccac
     2101 caactcccgc attgtagttc atgccatcga cggagtcttg tcccccaaca acgaataata
     2161 aacagttatg aaaagaataa agatcacagt agtttgcctg ctatgtctgt cgtggactct
     2221 gacagcctat gcacagcaaa tcattgtccg ggggctggta acttcggaag accatgagcc
     2281 gctgatatcc gttaccatcg cagagatcga cagaaacaat cgtattgtga acggaacggt
     2341 cagtaattac gacggtgaat acgtgttgca agtcagagac aaggccaata agctgcgctt
     2401 taattacgta ggattcaaag ctcaagatca ggagattggt gaacgaagcg ttatcaatgt
     2461 gaccatgcag gaagaatccc tggaattggc agaggctcaa gttaccggca aagcggtcac
     2521 caatacgggt accatgagca tacccacccg cgaaatctct tttgccatgc aaaaggtcga
     2581 tgctaaagaa ttcgaaggca tccaggtgac ctctgtcgaa gacgccctgc agggaagggt
     2641 gagcgggctg gacatcgttt ctaactcggg agctcccggc tcgggtatgt ctatgcgcat
     2701 ccgcggtacc acttcgctgc gcagcgacgg ttcacaaccc ttgattgtgg tcaacggcgt
     2761 tcccttcgaa accactgtgg cctccgactt tgattttgcc acggcccgcg aagaacaata
     2821 cgccgaattg ctcaccgtca accccgacga cattctcgaa atcgtggtac tcaaagacgc
     2881 agcctccacc gccatctggg gagcgcaggg agccaacggc gtcttacaaa tcaccactaa
     2941 aaaaggtgcc cggggcaaac ccaaggttca gtatacctac cgtctgactt cggcctttat
     3001 gcccgacccc attcccctac tcaacggaga cgaatacacc atgcttatga aagaagagta
     3061 cctcaacccc accctgaaca ccagtcaggg gaacattccc gaatacggtt atgtgcagga
     3121 cggctccgct ttcttttgga actacaacaa caacaccgat tgggtaaaag agatcacccg
     3181 ggcagcctgg aaacacgaca acaacctgtc tatcagtgga ggaggcgaac gaggcgacta
     3241 ccgaatctcg ttcggttacc tggacgaaga gggcattgtt tacggacaag gtctgaggcg
     3301 cttcaccaac agaaccgacc tgggctacgt actttccgac agattgatct tcagaacgga
     3361 attggcttac accttttcgg acaacgacag aaattacgac ggagatatac tgaataaagc
     3421 cttgcgtaag atgcccaacg tacctgtgta cacccgggac gaaaagggca attacaccga
     3481 tgtctatttc aacatcgacc gggcctcgtc caatttgcac gccgatcaac gcgatatggt
     3541 caatccggtc ggtctggtca atctggccag ctataattcc aaaagctacc gtatcatgcc
     3601 cactttcagg ctgcaatacg acattcttga tcccggaaag ggcctgctca ggtttaaatc
     3661 gtccgtttcg atggatgtga acagcatcca taccaaccgc ttctttccca aagaactttc
     3721 gggagccaat tacgacgata cttccatcaa ctatgctttc gacggcgacg aagaaagcat
     3781 caatattcaa ctcgacaaca accttacctg gcaacccagg ctaagcaacg gccatgcctt
     3841 tatggtctac ggagccgtgc agaccagcat caacaagggc agttcacaga gtttaggttc
     3901 ttattgggct ccttcgtcgt acattcagaa tgtgtcggaa ggttttactt cagaaaccaa
     3961 atcctattcc agcgaatcgc acggctacgg tatgtttgta tcctcgcact acgcctatct
     4021 ggaacgttac attgtagact ttgtggtgcg ccgcgatgtg agttcccgtt tcggaaaaaa
     4081 caaccgtgta ggttatttcc ccggtatttc cctgaaatgg atcgcctcgg acgagaattt
     4141 tttgcaggat gtttcctgga taaatatgct ggctttcagg cccagctggg gcatttcggg
     4201 caataccccc aaatcgt
//
LOCUS       DAOSGL010000202         4215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_19021_length_4215_cov_4.023317, whole genome shotgun sequence.
ACCESSION   DAOSGL010000202 DAOSGL010000000
VERSION     DAOSGL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19021_length_4215_cov_4.023317"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="PKY68_06700"
     CDS             complement(<1..2226)
                     /locus_tag="PKY68_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24760.1"
                     /translation="MNQTAFLTGNIFKELKAVLASVFLFFMPLSARAAQEEVLLTFQH
                     TGIGNFYVNSLYDYESETVYLPFTELFSLLEINYQPDARNFTVKGNFLTPDQPYTINF
                     AAARASLGKEVFEFSLEEVRLTQTEFYLHPRVFEEIFGLNFTVNINHLMLTLETQHSL
                     PVQERMARERLREKMQGRDLIREDYPLLYGRERDLLDGAMLDYSITGNYSGDNRSLGY
                     SLVGGMELLGGDLQGTFYGNHANGGYNSLDVSNLRWRYAILDNKYISGITLGQTSTTG
                     LQSVAIRGISISNDPIEPRQMYETYVVDGNTEPESEVELYINDRLSGFIRADELGYYR
                     FDVPLSYGTTRVNLRIYTPSGELIENDKQIQVPFTFLPKGVFTYNVQAGQAEDYAYDT
                     LRGQWLGHANVALGINRWLTASLGTQYSGADLSAGRMSYYGSVSARIAKQYLLSLDLA
                     PQEFYRIMSSVMYANNLSMNLQYTKYEAQSLFNTRRASDYLNANIYLPFKLFGAHSGI
                     RLGGEHTVFKNSSLSSLLGDFTQRIGKVNLRLNYRENFSYSNRQINPLQSLLSTTLTY
                     TVSRSPGLPVYIRGMYIRAQNQFDFRNLRLRETSLELSRTVFKIGRLDIGVAYNHIMN
                     QFSTRLGLIVDFEKMRSNTTLHTRGKSFTARQSLNGSIGYDATHRYFTADNRQQVGHS
                     ALSVNMYVDENNSGRYDEGEERLPFQGVRVDRTARMKVGRDSVLRVKQLMNYYRYNLS
                     VN"
     gene            complement(2273..3106)
                     /locus_tag="PKY68_06705"
     CDS             complement(2273..3106)
                     /locus_tag="PKY68_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24761.1"
                     /translation="MKSLRTLLILLVCFGLAPLMWAQVALAPSFVFIDENSGVGNVFV
                     SNNSQEAYEISIDFAFGYPDSDAEGNLVMNYEDSGAYAIHALDEMIRAFPRSFVLQGG
                     EQRTVRIQVLPNQRRKEGAFFTRMKVLAQPQAREVTETVVEEGIGTRITFNFEQVTAV
                     FYHKGRVSTGVEIKGVDVRQNEGVLELRPHLLRSGNAPFLGSMYARLKDKNGRVLAET
                     QSTTTVYFDEIRRIDLDLEGVETGSYELELSFETKRNDMMASDLVQAARVVHVQEVRV
                     N"
     gene            complement(3269..3844)
                     /locus_tag="PKY68_06710"
     CDS             complement(3269..3844)
                     /locus_tag="PKY68_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24762.1"
                     /translation="MKKTMKTTMKIMAAMAVMMGFAFNAMGQSNTDNDAIAGKAQVLQ
                     AMEVTKQVDLDFGMVSQGSAKTIGLTNNVTGTPNLGTQTTGRFLVSAAAGTSVNLTFT
                     TPTNLVYDVNNLPIGTYVCGYANDNAYSSSAVSFGAPGTVTKDIASFPTNDVGSSVNG
                     VYVFIGATVSPTSSQAVGSYTANITLTATYN"
     gene            complement(3990..>4215)
                     /locus_tag="PKY68_06715"
     CDS             complement(3990..>4215)
                     /locus_tag="PKY68_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24763.1"
                     /translation="VQLAYRNDGTGLMPQETGGDYLFFPLSDSGLLIEHMKDSPQVLV
                     ASVFVQVTIAAPALTQHIYRGNIHLQLEYN"
BASE COUNT         1140 a         1029 c          956 g         1090 t
ORIGIN      
        1 attcacgctg agattgtagc gatagtaatt catcaattgc ttaacacgca acacgctgtc
       61 gcggcccact ttcatgcggg ctgtccggtc cactctgaca ccctggaaag gcaggcgttc
      121 ttcgccctcg tcgtaacggc cggaattgtt ttcgtccaca tacatattca ccgacaaggc
      181 ggaatgtcct acctgttgac ggttgtcggc cgtaaaataa cggtgcgtag cgtcgtagcc
      241 tatactgccg ttcaggctct ggcgggcagt aaagcttttg cctcgtgtat gaagggtagt
      301 attgctgcgc atcttttcga agtcaacaat caagcccaga cgggtactga attgattcat
      361 aatatggttg taggccacac caatgtccaa gcggccgatc ttaaacaccg tacgcgaaag
      421 ctccaacgaa gtttcccgca agcgcagatt cctaaaatca aactgattct gagcccgtat
      481 atacatgcca cgaatataca ccggcaagcc cggcgaacga gaaacagtat aggtaagcgt
      541 agtgctcagc aagctttgca aaggattgat ttgccgattg gaataggaaa agttttcgcg
      601 ataattaaga cgcaggttta ctttaccgat gcgctgggtg aaatcgccca aaagtgaact
      661 caggctgctg tttttgaaca ccgtatgctc cccacccagg cggatacccg aatgagcgcc
      721 gaaaagttta aagggaagat agatattggc gttcaggtag tcgctggctc tacgggtatt
      781 gaacaaactt tgggcttcgt acttggtgta ctgaagattc atgctcaaat tgttggcata
      841 catcacactg ctcatgatgc gatagaattc ctgaggggcc aggtcgagac tgagcaaata
      901 ttgtttggca atgcgtgccg acacgctgcc gtaatacgac atcctgcccg cagacaaatc
      961 ggcgccgctg tattgtgtac ccagcgaagc ggtcagccag cggttgatac ccaaagccac
     1021 attggcatgc cccagccact gccctcgcaa cgtatcgtaa gcataatctt cggcttggcc
     1081 ggcttgcacg ttgtaagtga acacgccctt gggcagaaaa gtaaaaggca cctgaatctg
     1141 tttgtcgttt tcgatgagtt cgcccgaggg cgtgtagatg cgcagattca cccgggtagt
     1201 accgtaactc agaggcacat caaagcggta ataacccagc tcatccgcac gtataaagcc
     1261 cgacaggcgg tcgttgatat agagttccac ttccgattcc ggctcggtgt tgccgtccac
     1321 cacgtaggtt tcgtacattt gacgaggctc aatgggatcg ttgctgatag aaatacccct
     1381 gatagctacc gactgcaagc cggtcgtgct ggtctggccc aaagtaatac ccgaaatgta
     1441 tttattgtcc aaaatagcgt agcgccaacg taaattactt acatccagag aattatatcc
     1501 tccgttggcg tggttaccgt agaatgtccc ctgcaaatcg ccccccaaca gttccatacc
     1561 tcccaccagg ctataaccta agctccggtt atcgcccgaa taattacccg taatggaata
     1621 atccagcata gccccgtcca gcaaatcgcg ctctcggccg taaagcaagg gataatcttc
     1681 gcggatcaga tcgcggccct gcattttttc gcgcaggcgt tcccgggcca tacgttcctg
     1741 caccggcagg ctgtgttggg tttcgagcgt aagcatcagg tgattgatat tcaccgtaaa
     1801 gttcaaacca aaaatttctt cgaatacgcg gggatgcaga taaaattcag tctgggtcaa
     1861 acgcacctcc tccagactga attcaaaaac ttctttgccc aggctggccc tagctgcggc
     1921 aaaattaatg gtgtaaggtt gatctggtgt taaaaaattt cccttgaccg taaaatttcg
     1981 ggcatcgggc tgataattga tttcgagcag cgaaaagagt tcggtaaaag gaagatacac
     2041 cgtttcgctt tcgtaatcgt ataagctgtt gacgtagaaa ttacctatcc ctgtgtgttg
     2101 gaaggttaac agaacctctt cctgtgcagc tctcgccgaa agaggcataa aaaacaagaa
     2161 gacagaagca agaacagctt ttagctcttt aaaaatattg ccggtaagaa aagctgtttg
     2221 attcataggg aaataaagaa aaaagcaccg gcaacaaggg atgcgcagga gcttaattaa
     2281 cccttacctc ctgcacgtgc acaacacggg ctgcctgcac caaatcggag gccatcatat
     2341 cgttgcgttt cgtttcgaaa cttaattcca actcataact acccgtttcc accccttcga
     2401 gatcgagatc gatacgacgg atttcatcga aatatactgt agtggtgctt tgagtttcgg
     2461 ccagcactcg gccgttttta tctttaagac gagcatacat actgcccaga aaaggggcat
     2521 taccgctgcg caacaaatga ggccgtagtt ccagcactcc ttcgttttga cgtacatcca
     2581 cccccttgat ttccacaccg gtactcaccc tgcctttgtg ataaaacaca gccgtaacct
     2641 gttcaaaatt gaaggtgatc cgggttccta tgccctcttc gactaccgtt tcggtcactt
     2701 cccgggcctg aggttgagcc aacactttca tacgggtaaa aaaggcacct tccttgcggc
     2761 gctgattggg taatacctgg atgcggacgg tgcgctgttc gcctccctgc aacacaaaac
     2821 tgcggggaaa agcccggatc atctcatcga gggcatgtat ggcataggcc ccgctgtctt
     2881 cgtaattcat caccaaatta ccctcggcgt cgctgtcggg ataaccaaag gcaaaatcga
     2941 tggaaatttc gtaggcctcc tggctattgt tgctcacaaa aacattgccc actcccgaat
     3001 tttcgtctat aaacacaaaa gagggagcca aggctacctg ggcccacatc agaggggcta
     3061 aaccgaagca taccagcaaa atcagcaaag ttcttaagga tttcatgcgg cttagattaa
     3121 aactttatta caaccatctt tagcccggtt aaggcttgat ttaaccggtt ccaaatatac
     3181 aaaaaattgc gttacaacaa gttttacgca aaaaacataa aaaatcatag ggcaacccga
     3241 ggtcgcccta tgattaaaaa aatgaatatt aattataggt ggcggtgagg gtgatgttgg
     3301 cagtgtaaga tccaacagct tgacttgaag taggcgaaac tgttgcaccg ataaatacat
     3361 atactccatt cacactagaa cccacgtcat tagtaggaaa actggcgatg tctttagtta
     3421 ctgtgccagg cgcaccaaaa ctgacagcac tagaggagta agcattgtcg tttgcataac
     3481 cacatacata agtaccgatt ggcaaattat tcacatcata aactagatta gtaggtgtcg
     3541 taaacgtcag gtttacactg gttccggctg cagctgatac cagaaaacga cctgtcgttt
     3601 gagttcctaa attgggtgtt cctgttacgt tgtttgttaa tcctattgtc tttgcagaac
     3661 cctgagatac catcccaaaa tccagatcaa cttgctttgt cacttccata gcttgcagaa
     3721 cttgcgcctt accggcaata gcatcgttat ctgtattgct ctgccccatc gcattaaaag
     3781 cgaaccccat catcacagcc atggcggcca tgattttcat tgttgttttc attgtttttt
     3841 tcatactcat tttcattttc ttgttagtaa ttgagtttac ttttttcgtg ttttgtttga
     3901 tgttttatgt tctaatcttt gttttaggtt gataaaagca tttttataca tacattcaga
     3961 aacaaatgcc gtgccaaaga tacaattttt taattatact ccagttgcaa atgaatattt
     4021 ccccgataaa tatgttgggt taaggcagga gcggcaatgg ttacctgcac aaaaacggag
     4081 gcaacgagta cctggggact gtctttcata tgttcaatga gcagaccgct gtcgctcaag
     4141 ggaaaaaata aataatcgcc gccggtttct tggggcatca gaccggttcc gtcgttgcgg
     4201 taggccagtt gaacc
//
LOCUS       DAOSGL010000203         4182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_19223_length_4182_cov_3.065907, whole genome shotgun sequence.
ACCESSION   DAOSGL010000203 DAOSGL010000000
VERSION     DAOSGL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19223_length_4182_cov_3.065907"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4182
                     /gene="sprA"
                     /locus_tag="PKY68_06720"
     CDS             <1..>4182
                     /gene="sprA"
                     /locus_tag="PKY68_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOH24764.1"
                     /translation="YPVPRQAPLSYQDLVRPVMGDLKNPENMETTIEYDLNSGDYVVR
                     TRIGGTDVSTPISLTPEEFRAYWDAKTRADFFKKKNQIDYSKKKEAFSLTDLQFDIGA
                     AEKIFGPGGVQLKTQGSAEVSFGVKNNIVNNPSLSERARNRTFFDFDQSIQLSVNGKV
                     GDKINVNMNYDTEATFDFDSKAIKLRYEGKEDEIIKSLEAGNVSMPVKNSLINGASSL
                     FGVKTQLQFGKLNIAAVLSQQNTQTKSASLSGGVQTTPFEISVDAYDENRHYFLSHYF
                     RDNYDRWMENLPYISSGVVVNKVEVWVTNKTAVMDNARDILAFADMAEPERIYNAYWT
                     KGQNNYPSNDANNLYAEMVENYAEARQINVSATVLEPLAAQGIIIGNDYERLESARRL
                     EPTEYKLNASLGYISLNQALRSDEVLAVAFEYTKNGKVYQVGEFSTDGIESPKALLVK
                     LLKGTDFSPRAPYWDLMMKNVYALGAYSMSKDNFKLNISFQNDTLGLYVNYISEGKIA
                     NQILLRVMNLDRLNLNRDPIPDGQFDYVEGYTVMSSNGRIIFPVLEPFGSHLRKAIGD
                     DALAEKYIFQELYDSTLTVVQQLTEKNKFRIEGEYKSSQGGADISLGTMNVTRGSVKV
                     TAGGILLTENVDYVVDYNSGTVSIINENLLSSGTTINATCEDQGIYNMVRKSLAGFNM
                     EYAFSKDFSVGATLMHLSEKPLTNKVDMNTEPLNNTIMGLNTGFTVQSQGLTNLIDKL
                     PLINVTKPSQLTVKAEVAQLKPGASKQIDNTVYVDDFETAKKTFSLRDVTQWFLASTP
                     YDPMGGLFPEAAYSNDLRYGMNRALMSWYTIDQIFTQSRSQTPDHIRNDYEQLSNHYV
                     RAVNEKEIYPDRDLQFNQTGLLSVMNISYYPKQRGPYNFDTEGMQADGTLANPEQRWG
                     GIMRRIESSMTNFEAANVEYIEFWLMDPFIYDSLGVNTGGDLYFNLGEISEDVLKDGR
                     KSFENGLPMGGDQSYVSETVWGKVSNKTVNVYAFDNTPGIRRVQDVGLDGLNDEEEAV
                     KRADYLNALENSLNPDVLERWRNDPFSPLNDPSGDNFHYYRGSDYDRSKTSILDRYKY
                     YNGTQGNSQASADTGESYETASTTLPDVEDINADFTLNETERYYQYKVSLRKQDLEIG
                     QNYVNDKRTSVVRLKNGKSEQVTWYQFKIPIRDYDKKIGTINGFNSIRFIRMFLTGFQ
                     DSTVLRFGSLDLVRGDWRVYTKDLAPAGVVPASETMMEVTTVDLEENSSREPIHYVLP
                     PGVEREGDPSQPGVYQQNEQSLALRIRQMAPGDARAVYKNTGLDFRQYERLQLFVHGE
                     ALPEDLNPPANYEMTVFIRLGSDYQNNYYEYEVPLKISQPHVHNPESVWPEENFIDLP
                     FELLTSIKNRRNSQPG"
BASE COUNT         1192 a          996 c         1003 g          991 t
ORIGIN      
        1 tatcccgtac ccagacaggc cccgctctcg taccaggacc tcgtcaggcc ggtgatgggt
       61 gacctgaaga atcccgaaaa catggaaaca accatagaat acgacttgaa tagcggcgat
      121 tatgtggttc gtacccgaat tggagggact gatgtgagta ctcccatcag tcttactccc
      181 gaagaattca gggcttattg ggatgctaaa accagggccg atttttttaa gaaaaaaaac
      241 caaatcgatt acagcaaaaa aaaggaagct ttttctttga ccgacttgca gtttgatatc
      301 ggagctgccg aaaaaatttt cggacccggt ggggtacaac tcaaaaccca gggttcggcc
      361 gaagtgagtt tcggtgtcaa gaataacatc gtcaacaatc cctctttgtc ggaacgggcc
      421 cgcaaccgta ctttttttga ttttgaccaa agcatacaac tcagcgttaa cggtaaggtg
      481 ggcgataaga taaacgtcaa catgaattac gacaccgagg ctacttttga ttttgattcc
      541 aaagccatca aactgcgtta cgaaggcaag gaagacgaaa tcatcaaaag cctcgaagcc
      601 ggtaatgtga gtatgcccgt caagaattcc ctgatcaacg gagccagttc attgtttggt
      661 gtcaagactc agttgcagtt tggcaaactc aacatcgccg ccgtgctttc gcagcaaaat
      721 actcaaacca aatcggccag ccttagcgga ggcgtacaga ccactccctt tgagatttcc
      781 gtggatgcct acgacgaaaa ccggcattat tttctttcac attatttcag ggataattac
      841 gaccgctgga tggaaaacct gccctatatt tcctcgggcg tggttgtaaa caaagtggaa
      901 gtctgggtga ccaataaaac ggcggtcatg gacaatgccc gcgacattct ggcttttgcc
      961 gatatggccg agccggaacg gatatacaat gcctattgga caaaaggaca gaacaactat
     1021 ccttcgaacg atgccaacaa cctctatgcc gaaatggtgg aaaattacgc cgaagccagg
     1081 cagatcaatg tttctgcaac ggtgctggaa ccattggccg cccaggggat cattatcggc
     1141 aacgattatg aacgcctgga aagcgcccgc cgtcttgaac ccaccgaata caagctcaac
     1201 gccagtctgg gttatatcag cctgaatcag gctttgcgtt ccgacgaagt gttggccgtg
     1261 gcttttgaat acaccaaaaa cggcaaggtt taccaggtgg gtgaattttc gaccgatggg
     1321 attgagtcgc ccaaggcgct gctggttaag ttgctcaaag gtaccgattt ctcgccgaga
     1381 gctccctatt gggacttaat gatgaaaaac gtatatgcct tgggggctta cagtatgtcg
     1441 aaagataatt tcaaactgaa catcagcttc caaaacgata cgctgggatt gtacgtcaac
     1501 tatataagcg aaggaaagat tgccaaccag attttattga gggtgatgaa cctcgaccgc
     1561 ctgaacctga atcgcgatcc tattcccgac gggcagttcg actatgtgga ggggtatacg
     1621 gtgatgtcgt ccaacggccg aatcattttt ccggtgctgg agccttttgg ttctcacttg
     1681 cgcaaagcca taggcgacga tgccctggct gaaaaataca tttttcagga actctacgat
     1741 tcaactctga ctgttgttca gcagctgacc gagaaaaaca aatttcgtat cgaaggggag
     1801 tataagtctt ctcagggagg agcggatatt tcgctgggca cgatgaatgt cacccggggt
     1861 tcggtcaaag taacggctgg tggaatctta cttaccgaaa acgtcgatta tgtagtggat
     1921 tacaattccg gaacggtgag tattataaat gagaatcttt tgtcttcggg aaccaccatc
     1981 aatgccacct gcgaagatca aggcatctac aatatggtgc gcaaatcctt ggccggcttc
     2041 aatatggaat atgctttcag taaggatttc agtgtgggag ccaccttgat gcatctttcc
     2101 gaaaaacctc tcaccaacaa ggtagatatg aataccgagc ccctcaacaa caccattatg
     2161 gggcttaaca ccgggtttac cgttcagtcg cagggcctga ccaacctgat tgataaactg
     2221 cctctgatta acgtgactaa accttctcaa ttaacggtca aggccgaggt ggctcaattg
     2281 aagccgggcg cctccaaaca aatcgacaat accgtttacg tggacgattt tgaaaccgcc
     2341 aaaaagactt tcagcctgag agatgttact caatggtttt tggccagtac tccttacgac
     2401 cccatgggag gactgtttcc cgaagctgcc tattccaacg atttgcgcta tggcatgaac
     2461 cgtgcactga tgtcctggta cactatcgat cagatattta ctcaaagccg ttctcaaacc
     2521 cccgatcata tccgcaacga ctatgagcaa ttgtccaatc actatgtgcg ggcggtcaac
     2581 gaaaaagaaa tctatcccga ccgtgatttg cagtttaatc agacgggttt gttgtcggtg
     2641 atgaatattt cttattaccc gaaacaaagg gggccgtata atttcgatac ggaaggcatg
     2701 caagccgatg gcaccctggc caatcccgaa caacgctggg gaggaatcat gcgcagaata
     2761 gaatcgagca tgaccaattt cgaagcagcc aatgtggaat acattgaatt ttggttgatg
     2821 gacccattta tttacgacag tctgggtgtg aacaccggag gcgatttgta tttcaacttg
     2881 ggtgaaattt cggaagatgt gctcaaggat ggccgcaaat ctttcgaaaa cggcctgcct
     2941 atgggcggcg atcaaagcta tgtgtccgaa accgtctggg gtaaggtatc caacaaaacc
     3001 gtaaatgtgt acgccttcga caataccccg ggaatcaggc gtgttcagga tgtaggcctg
     3061 gacggactta acgatgaaga agaggccgtt aaacgagccg actacctgaa tgccctcgaa
     3121 aattccctga atcccgatgt actggaacgc tggagaaacg atcctttttc gcccctgaac
     3181 gatccctcag gcgataattt ccactattac cgcgggagcg attacgaccg atcaaaaacc
     3241 agtattctgg atcgctataa atactacaac gggactcagg gtaattccca ggccagcgcc
     3301 gatacgggag aaagctacga aacagcttcc actaccttgc ccgatgtgga agacattaac
     3361 gccgacttta cgttgaacga aacagaacgt tattaccaat ataaagtgtc gctgaggaag
     3421 caagacctgg aaataggcca aaactacgtg aacgacaaaa ggacctcggt ggtgcgcctc
     3481 aaaaacggca aatcagaaca ggtcacctgg tatcagttta aaatacccat cagagactac
     3541 gacaaaaaaa taggcaccat caacggattc aacagcatcc gctttatacg gatgtttttg
     3601 accggttttc aagacagtac tgttttgcgt tttggcagtt tagatctggt tagaggggac
     3661 tggagggttt ataccaaaga cctggctcct gcaggcgtgg tacctgcttc cgaaactatg
     3721 atggaagtca ctacagtcga tctggaagag aactcttcgc gagaacccat acattatgtc
     3781 ttgcctcccg gagtagagag ggagggagat cccagccagc cgggagtata tcaacaaaac
     3841 gaacagtctc tggctttgag aatcaggcaa atggctccgg gcgatgcccg cgctgtctac
     3901 aaaaatacag gcttggattt cagacaatac gaacggctgc aattgtttgt acacggtgaa
     3961 gccttgcccg aagacctgaa tccgccggcc aattacgaaa tgacggtttt cattcgtttg
     4021 ggctccgact accaaaataa ttattacgaa tacgaagttc ctctgaaaat ttcacagccc
     4081 cacgttcata atcctgaatc ggtttggccc gaagaaaact ttatagattt gccttttgag
     4141 ttgctcacca gcatcaaaaa caggcgcaac tcacaacccg ga
//
LOCUS       DAOSGL010000204         4134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_19554_length_4134_cov_5.219662, whole genome shotgun sequence.
ACCESSION   DAOSGL010000204 DAOSGL010000000
VERSION     DAOSGL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19554_length_4134_cov_5.219662"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..417
                     /locus_tag="PKY68_06725"
     CDS             4..417
                     /locus_tag="PKY68_06725"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOH24765.1"
                     /translation="MINGPNLNLLGKREPDIYGSQNFESYLEILRKDYPQVEFEYFQS
                     NHEGAIIDKLHEIGFSYDGIVLNAAGYTHTSIAIGDAIRAIQTPVIEVHISDIYKREE
                     YRHVSRTEAACIASFVGYGMESYRMAVEAFLEYIS"
     gene            429..1061
                     /locus_tag="PKY68_06730"
     CDS             429..1061
                     /locus_tag="PKY68_06730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOH24766.1"
                     /translation="MDELLEKYICSRIDPEPELLKELRRETYVRFLNPRMSSGHLQGR
                     VLAMLCKMIRPRRVLELGTFTGYSTLCLAEGLPEGSRILSIDRNDELEEHLKTWFSRS
                     ELGSKIDLLFGEALDLIPKLTESFDLVYMDIDKRDYKSCYDLLLQHQPSGTFILIDNT
                     LWNGKPMREISCKDTQSLAIADFNDYIAADSRVEKIILPIRDGLTVLLIK"
     gene            1082..1417
                     /gene="rbfA"
                     /locus_tag="PKY68_06735"
     CDS             1082..1417
                     /gene="rbfA"
                     /locus_tag="PKY68_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOH24767.1"
                     /translation="METIRQNKISRLIQKELSEVFREQTTALPGTMVTVSMVRVSPDL
                     SVAKAYLSLFPPEKSKEMLANIQNSAKSIRYKMAQRLRFQLRRMPELSFFLDDSLDYA
                     QNIEDLLKK"
     gene            1414..2631
                     /locus_tag="PKY68_06740"
     CDS             1414..2631
                     /locus_tag="PKY68_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH24768.1"
                     /translation="MNLAFHIARRYLFSKKSHNAINIISMVSVFGVAAATIAMVCTLS
                     VFNGFQDLLTGLYKNFEPELKIRAFEGKTFRLNSDFDLIRAHKEVAVFCESLEENALI
                     RYKGSQASALVKGVSDNFSELANMDSLIYAGKFVLEEEGFQFASIGIGLASLLGTGSS
                     FIDPVTLYAPKRTGSLNPLNPASAFKTRRILLTATFGIDQPEYDDRIIIVPLNFAREL
                     FEYTDELSSVELKLKKGANLQRVKEDFRQLLGPDFIVEDLKEQKADIYRINRVEKWIT
                     YLILCFILLIALFNVIGSLSMLILEKKQDAGILKRLGADHKTIRKVFLYEGVMIAFLG
                     AFSGILLGVLLCLIQQHFGLLKLGSGNFIVGSYPVLVKASDLFFILLTVLAVSLPSVW
                     WPVHYYFKNDELP"
     gene            2615..>4134
                     /locus_tag="PKY68_06745"
     CDS             2615..>4134
                     /locus_tag="PKY68_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOH24769.1"
                     /translation="MTNFLKIAYGRTLVITGLISWLFVLDFSPLQAQRSYGGRPLSME
                     EEVAYVRALADYLVELPSFDVDSLLREDSIYHNPNGALRFAHKMFVNLSPENSGITFN
                     LEDGTKIWKLGLRSEGAYSLNVIFEEYIVPPGARLYLYNPDKTQVLGSFTSDNMQESG
                     EFAIAPVSGEEIMIEYHEPADAAFPGRLRLTEVNHDYRGPMLLGTKFDKLNLPCVPEL
                     SCQAEYDSIGRSICLLILNGTTYCTGTFVNNTACDGRPLLLTAAHCLENNPSLAGRTV
                     VFMNYLSPRCMHQIRGSEEFSLSGCQALAFSEETDYLLMELKEKIPPDYRLYLAGWSR
                     DTSARYESPFACLHHPYGEGKRYAQTTDSLTYATWPYPGDGFDPDSHWSVRQWQEGHT
                     WTGSSGAPLLNKHHQLIGFLTGGDSGGSSGCGPYQLGDFFVRFQKAWDYESDSLNQLA
                     HWLDPLSSDALTISGLDPNQLNPARRITNLRPTDSIAGLRHENPENGFLFGHNSFE"
BASE COUNT         1084 a          915 c          977 g         1158 t
ORIGIN      
        1 gccatcatca atgggcctaa tctgaatctc cttggaaaaa gagaaccgga tatatacggc
       61 tcacaaaatt tcgaatcgta tttggaaatt ttgagaaagg actatcctca ggtagaattt
      121 gagtactttc aatccaatca cgagggggcc atcatcgaca aacttcatga aatcgggttc
      181 agttacgacg gcatcgtttt gaatgctgcg ggctacactc acacatccat tgcgattggt
      241 gacgccataa gagcaataca aacaccagta attgaagttc atatttcgga catttacaaa
      301 agagaagaat atcgtcatgt ttccagaacg gaagctgcgt gcattgccag ctttgtaggc
      361 tatggaatgg aatcgtaccg catggctgtg gaggcatttt tagaatatat ttcataattg
      421 tgtggtgagt ggacgagctt ttagaaaagt acatttgtag ccgtattgat cctgaacctg
      481 aattgttgaa ggaactcaga agagagacat atgtacgttt tttaaatcca aggatgtcgt
      541 caggtcattt gcaggggcgg gtgctggcca tgttgtgcaa aatgatccgc cctcgaaggg
      601 ttctggagct gggaactttt accggctatt ccacgttatg cttagccgag gggttacccg
      661 aaggttcacg cattctgagc atagaccgca acgatgaact ggaagagcac ctgaaaactt
      721 ggttttcaag atcggaactg ggctcaaaga tcgatcttct ttttggagaa gcgcttgatt
      781 taattcccaa actgaccgaa tctttcgatt tggtgtatat ggatattgac aaaagagact
      841 acaaaagttg ttatgactta ttgcttcaac accagccaag cggcactttt attttgattg
      901 ataacacttt atggaacgga aagcccatgc gggaaatttc ctgcaaagat actcaaagcc
      961 ttgccattgc agattttaac gattatattg cagcggattc ccgggtagaa aaaatcatac
     1021 tacccatccg agacgggttg accgttctac ttataaaata aaaacacata aaaacagaga
     1081 gatggaaact atcagacaaa ataaaataag ccggcttata caaaaagaat tgagcgaagt
     1141 gttccgggaa cagaccacag cgcttccggg caccatggtc acagtcagca tggtccgtgt
     1201 cagtcctgat ctgagtgtgg ccaaggctta cctgagtctt ttcccacccg aaaaaagcaa
     1261 agaaatgctc gccaacatac agaacagtgc caaatccatc cgctataaga tggcccaaag
     1321 gttgcgcttt cagctaagaa ggatgcccga attgagtttc tttctggacg attcactgga
     1381 ttatgctcaa aacatagaag atttgctcaa aaaatgaatc ttgcattcca tattgcccgt
     1441 cgttaccttt tttcaaaaaa atcacacaat gccatcaata ttatttcgat ggtttcggtt
     1501 tttggggttg cggcggccac tatagcaatg gtttgtactc tttcggtttt caacggtttt
     1561 caggatttgt tgacgggtct gtataaaaac tttgaaccgg agcttaagat tcgtgccttt
     1621 gaaggaaaaa ccttcaggct gaattccgat tttgacctta tacgggccca taaagaggtg
     1681 gctgtctttt gtgagagctt agaagagaac gccctgatca gatataaagg aagccaggcc
     1741 agcgctttgg tcaaaggcgt ttcggacaat ttttccgagc tggccaatat ggacagcctt
     1801 atttatgccg gtaaatttgt tctcgaagaa gagggttttc aatttgccag cattggcatc
     1861 ggcctggcaa gtttgttggg taccggaagc tcctttattg atcctgtaac tctttacgct
     1921 cctaaaagaa caggttcgtt gaatccgctg aatccggcat cggcctttaa aacccggcgt
     1981 attttactaa cggccacttt tggcattgac caacccgaat acgacgaccg gatcattatc
     2041 gtgcccctga attttgccag agaactgttt gaatataccg atgaattgag ttccgtggag
     2101 ttgaaactaa aaaaaggagc taaccttcaa cgggtaaaag aggattttcg tcagcttttg
     2161 ggcccggatt ttattgtaga agacctaaaa gaacaaaaag cagatattta ccgaattaac
     2221 cgggtagaaa aatggatcac ctacctgatt ttgtgtttta ttttgcttat tgccctgttt
     2281 aacgtgatcg gctccctgtc tatgttgatc ctcgaaaaaa agcaggacgc cggcattctt
     2341 aagcgtcttg gtgccgatca taagaccatc cgtaaagtgt ttttgtacga aggcgttatg
     2401 atcgcttttt tgggagcttt ttcgggcatt ttgctgggag ttttgctttg cctgattcag
     2461 caacattttg ggcttctcaa actagggagc ggtaatttta ttgttggttc ctatcctgtg
     2521 cttgttaagg caagcgattt gttttttatt ttgctcaccg tcttagctgt cagtctgccc
     2581 tctgtgtggt ggccggtaca ttattatttt aaaaatgacg aacttcctta agatcgctta
     2641 tgggagaact ctggtgatca caggtctaat ctcctggttg tttgttttgg atttttctcc
     2701 tttgcaagcc cagcgttctt atggaggcag acctttgtcc atggaagagg aagtggcgta
     2761 tgttcgagcc ctggccgatt atttggtgga attaccttcc tttgacgtag actctctgct
     2821 tcgtgaggat tcgatttatc acaatcccaa cggggcgttg cgttttgccc ataaaatgtt
     2881 tgtaaatctg agtcctgaga attccggcat tacctttaat ctggaagacg gtaccaagat
     2941 atggaaactg ggcttgcgct ccgaaggagc ctacagcttg aatgtgattt ttgaagagta
     3001 tattgtgcct ccgggggccc ggctctattt gtataacccc gataagacac aagtgttagg
     3061 atctttcact tcggacaata tgcaggagag tggtgaattt gccattgctc cggtttccgg
     3121 cgaagaaatt atgatagaat atcatgaacc ggccgatgcg gcctttcccg gcagactccg
     3181 tctcaccgaa gtgaatcacg actaccgtgg cccgatgctt ttaggaacca aatttgataa
     3241 attgaatttg ccctgcgtgc ccgagttgag ttgtcaggca gagtacgaca gcatcgggcg
     3301 cagcatttgc ctgttgatac ttaacggcac cacttattgt accggaactt ttgtaaacaa
     3361 tacagcctgc gacggacgtc ctcttttact tacggctgct cattgcctcg aaaacaatcc
     3421 ctcattggcc ggcaggacag tggtctttat gaattacctt tctcctcgat gcatgcatca
     3481 aatcaggggt tccgaagagt tttccctttc gggttgtcag gccctggcct tttcggaaga
     3541 aaccgattac cttttgatgg aactgaagga aaaaatacca cccgactacc gcttgtatct
     3601 ggccggctgg agccgggata cgtcagcgcg ttacgagtca ccctttgcct gccttcacca
     3661 tccttacgga gaaggaaaac gctacgcgca gaccacagac tctttgacct atgctacctg
     3721 gccttatccc ggagacggat tcgatcccga cagccactgg tctgtaaggc aatggcagga
     3781 gggacacacc tggaccggct cttcgggagc ccccttgctg aacaaacacc atcaactgat
     3841 cggatttctg accggaggcg attccggggg aagcagcggt tgcggcccct atcagttggg
     3901 tgattttttt gtccgcttcc aaaaggcctg ggattacgaa agcgatagcc ttaaccaatt
     3961 ggcacactgg cttgacccgc tttcgagcga tgcactcacg ataagcggtc tggatccaaa
     4021 ccagcttaat ccggccaggc gtataaccaa tctcagacct acggattcta tcgccgggtt
     4081 acggcatgaa aatccggaaa acggttttct tttcgggcac aacagttttg agta
//
LOCUS       DAOSGL010000205         4116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_19677_length_4116_cov_3.559222, whole genome shotgun sequence.
ACCESSION   DAOSGL010000205 DAOSGL010000000
VERSION     DAOSGL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19677_length_4116_cov_3.559222"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..970
                     /locus_tag="PKY68_06750"
     CDS             35..970
                     /locus_tag="PKY68_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOH24770.1"
                     /translation="MNNRFLLPDLVRFILDTTEDLDKKEVETFVRAYFTVIEKALFEE
                     DFVKINNLGKFSLQKVEARKSINVNTGEAFEIKAHYKLTFVPDAKLREIANKPFEHLE
                     AIELENEQEPIKKETINPNKPTTKEDNIMEKKEKTVFVVTDKPAANEKKTAPETQKAK
                     KGKLVAWIVFVVVMCALIVFALWKTVFTGPEIEPTITEEVVESAPVAPVVEEVTPPAP
                     KVVDPADWETLKVVPLQKGKRLTLLALEYYGNKVFWVYIYEANRSHIKNPNKILAGTK
                     IRIPKPDPAVINAKDPELVRIAKEKETKYRAQFSK"
     gene            1101..2096
                     /locus_tag="PKY68_06755"
     CDS             1101..2096
                     /locus_tag="PKY68_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH24771.1"
                     /translation="MSQSVDIRELNELIEKKSSFVDMIVMGMDKVIVGQKHLVESLLI
                     GLLADGHILLEGVPGLAKTLAIKSLASLIKAKYSRIQFTPDLLPADVIGTMIYSQKSE
                     EFAVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGDKTYKLDAPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKVVIDYPKKEEERLIMRLNMGESLPEVKAVLKPEEIL
                     EARKVVRQVYMDEKIERYIVDIVFASRYPEEHGLKNLKEMISFGASPRASINLALAAR
                     SYAFIKHRGYVIPEDVRGICLDVMRHRIGLSYEAEANNLSSDEIIQEILNKVEVP"
     gene            2108..2977
                     /locus_tag="PKY68_06760"
     CDS             2108..2977
                     /locus_tag="PKY68_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOH24772.1"
                     /translation="METSDLLKKVRRIEIKTRGLSRNIFAGQYHTAFKGRGMAFSEVR
                     EYQYGDDVRTIDWNVTARFNHPYIKIFEEERELTVVLLIDVSGSRFFGTESLYKKDMI
                     TEIAATLAFSAMQNNDKVGVIFFSDKMEKFIPPQKGRKHILYIIRELIEFKPENNQTD
                     LSVALQYLTNVIKKRCTAFLISDFLVQQDFQTDLTLANRKHDVVAIQVYDQRETELPS
                     VGLIRVKDAETGREMLIDSASKNLRNNYRRWWEKQELRLDTTFKKSKVDAVSVSTAQD
                     YVKALMALFNLRN"
     gene            2983..3975
                     /locus_tag="PKY68_06765"
     CDS             2983..3975
                     /locus_tag="PKY68_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HOH24773.1"
                     /translation="MPKRIPILLLFSLLTFPAVLGQDVVVEAGLDSSMIWIGNPTTMR
                     LSVSAPRDLSVVFPDFKADTLVRGIQVLKRGPVDTVLLDNDRFRLQKNYLLTSFDAEL
                     YYIPPIEFKVGGVNYESNHLSLKVISFEVDTASMELFDIKGVENPPFVIWDYALPAGY
                     VLGGLLLLLLGYYLYKRFRKTKQITEVEPDPELLLPPHIVALQALDKIKSEKIWTEGK
                     YKLFYTQVTDVLRKYMGRRFEIPAMELSSSEILDFFRKNKEAQSVYEQLKQLLMLADF
                     VKFAKFQPLESENQQALNHAYGFVENTKPEECLNTEESEAEPKAGASVTDEGTV"
     gene            3998..>4116
                     /locus_tag="PKY68_06770"
     CDS             3998..>4116
                     /locus_tag="PKY68_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOH24774.1"
                     /translation="MIFAQPYYLFLVLLLIPAWVWYYLKRKKRYASLQVSNTFV"
BASE COUNT         1257 a          799 c          946 g         1114 t
ORIGIN      
        1 aacctgaaga acaaaatcaa acaaatagac tcagatgaac aatagatttt tacttccgga
       61 tttggtccgg ttcattctcg acacgacgga ggatctggat aaaaaagaag tggaaacttt
      121 tgtacgggct tattttaccg ttatagaaaa ggctttgttt gaggaagatt tcgttaaaat
      181 aaacaatttg ggtaaattca gcctgcagaa agtagaggcg cgcaaaagta tcaatgttaa
      241 cacgggagaa gcttttgaaa ttaaggctca ttacaagtta acctttgtgc ccgatgctaa
      301 acttagggaa atagccaata aaccatttga gcatctggaa gccatagaat tagaaaatga
      361 acaagaaccg ataaaaaaag aaacaatcaa ccccaataaa ccaaccacaa aagaagacaa
      421 catcatggaa aagaaagaaa aaactgtttt tgtagtaacg gataaaccgg ctgcaaacga
      481 gaaaaaaacg gcacctgaaa cacagaaggc caaaaaaggc aaactggttg cctggattgt
      541 ttttgttgtc gtcatgtgcg cactgatcgt ctttgcgtta tggaagactg tatttaccgg
      601 gcccgaaatc gaaccgacta ttacagaaga ggtagtcgag agcgctcccg ttgcgccggt
      661 agtggaggaa gtcactccgc ctgctcccaa ggtggtcgat ccggccgatt gggaaacact
      721 caaagtggtt cctctgcaaa aaggaaagcg ccttacttta cttgccttgg aatactacgg
      781 gaataaagtt ttttgggttt atatttacga agcgaacagg tcacatatca agaatcccaa
      841 caagattctt gccggaacca aaattcgtat tcccaaacct gatccggccg taataaacgc
      901 taaagatccc gagttagtac gtattgccaa agaaaaagaa acaaaataca gagcgcaatt
      961 ttctaagtaa aattcgtata aataacgaat tttgtctata aagataggtt tttttaacgg
     1021 ctaagctcct taaaactaaa cctatcttta taattttgtg ttccatctaa caaatatcaa
     1081 aatttaaaag ttttatagat atgagtcaaa gcgttgatat tcgagagtta aatgaactga
     1141 tcgaaaagaa gagcagtttt gtcgacatga ttgtcatggg tatggacaag gtgattgtcg
     1201 gacaaaaaca tttagttgaa tccctgctga taggcctgct ggccgacgga cacatattgc
     1261 tggaaggtgt gcccggtttg gcaaagacct tggccattaa aagtctggct tcacttatta
     1321 aagccaaata cagccggatt cagtttactc ccgatctttt gccggccgac gtgattggaa
     1381 caatgattta cagtcagaaa tccgaagaat ttgctgttaa aaaaggcccg atattcgcta
     1441 attttattct ggctgacgaa ataaacagag ctccggccaa ggtacagagt gctttgctag
     1501 aggctatgca ggaacgccag gtgactattg gagataaaac ctacaaactt gatgctcctt
     1561 ttctggtgat ggccacccag aacccaatag agcaggaggg tacctatcct ttacccgaag
     1621 ctcaggttga tcgttttatg ttgaaagtgg tgatcgatta ccccaaaaaa gaagaagaac
     1681 ggctcattat gaggctgaat atgggggaat ctttacctga agtcaaggcc gtactgaaac
     1741 ccgaagaaat tctcgaggcc cgcaaggtcg tccgccaggt ttatatggat gaaaaaattg
     1801 aacgctatat agttgatata gtattcgcca gccgttaccc tgaggagcat ggacttaaaa
     1861 atctcaaaga aatgatttca ttcggcgctt ctccgcgtgc cagtatcaac ctggctctgg
     1921 ctgcccgcag ttatgccttt atcaaacacc gtggttatgt tattcccgaa gacgtgcgcg
     1981 gtatctgcct ggatgtaatg cgtcaccgta ttggcctgag ttacgaagca gaggccaaca
     2041 atctgagtag tgatgaaatt attcaggaga ttctgaataa agtcgaggtg ccttaatgtt
     2101 ctgaagtatg gaaacgagtg acttactaaa aaaggttaga agaatagaaa tcaaaactcg
     2161 tggcctttca cgtaacattt ttgccggtca gtatcatacg gctttcaagg gacgaggcat
     2221 ggcattctcc gaggtgcgtg aatatcagta tggcgatgat gttcgcacta tagactggaa
     2281 tgtcacggcg cgatttaacc acccttacat taagattttt gaagaagaac gggaactgac
     2341 tgtcgtgtta cttatagatg tgtcgggtag tcgttttttt ggcacggaat ccctgtataa
     2401 aaaagatatg attaccgaaa tcgctgctac tctggctttt tcggccatgc agaataacga
     2461 taaggtaggg gtgatctttt tttcggataa gatggaaaaa tttattcctc cccaaaaagg
     2521 aaggaagcat attttatata ttatccgtga attgatagaa tttaaacccg aaaacaacca
     2581 aaccgacctg agcgtagctt tgcagtattt gaccaatgtc atcaagaagc gttgtacggc
     2641 ttttttaatt tccgatttcc tggttcagca agactttcaa accgacctga ctctggcaaa
     2701 tcgtaagcac gacgtagtag ctatacaggt ctacgatcag cgggagaccg aattgccttc
     2761 ggtgggtttg atcagggtta aagacgcaga aacaggcagg gaaatgttga ttgacagcgc
     2821 ttccaaaaat ctgcgaaaca attaccgccg ctggtgggaa aaacaagagc tgcgtctgga
     2881 tacaactttc aaaaagagta aagtagatgc tgtttcggtt tctactgctc aggattacgt
     2941 gaaggccctg atggccttgt tcaatctgag aaattgatcg ctatgcccaa aagaatacca
     3001 atcttattgc tgtttagctt attgacattt cctgctgtcc tgggacagga cgtagtggtg
     3061 gaagccggcc tggactcttc tatgatctgg ataggtaatc ctacaacgat gcgtctgagt
     3121 gtttcagcac cccgtgatct ttcggtagta tttccggatt tcaaggcaga cacgcttgtt
     3181 cggggaatac aagttctaaa gcgaggtccg gtggatactg ttttattgga caacgatcgg
     3241 ttcagattgc aaaaaaatta tttgttgacc tcttttgatg ctgaattgta ttatattcct
     3301 cccatagaat ttaaagtagg aggagtaaat tatgagagca accacttgtc gctcaaggta
     3361 atcagttttg aggtggatac ggccagcatg gagctgttcg atataaaagg agtagaaaac
     3421 cccccttttg ttatctggga ttatgctttg ccggccggtt acgtgctggg agggctttta
     3481 ttgttgttgc tgggctatta tttgtataaa cgattccgca aaaccaaaca aatcacggag
     3541 gtcgaacccg atccggaact tttgttgccg ccccatatag tagccttgca ggctttggat
     3601 aaaatcaaaa gcgaaaaaat ttggacagag ggtaagtata aattatttta tactcaggta
     3661 accgatgtcc tgcgtaaata catgggaagg cgctttgaaa ttccggccat ggaactcagc
     3721 agctccgaaa ttttggattt tttcagaaaa aataaagagg ctcagtctgt ttacgaacaa
     3781 ttgaaacaat tattgatgct ggctgatttt gttaagtttg ccaagtttca acccttggag
     3841 tcggaaaacc aacaagcctt gaaccatgcc tatggttttg tagagaatac taagcccgaa
     3901 gagtgcttga atacggaaga gtccgaagcg gaacctaaag cgggtgcctc ggtgacggac
     3961 gaaggcaccg tttaatgatt ttacagatgt ttatagtatg atatttgcgc agccctatta
     4021 tttgttcctt gtacttttgc ttatacctgc ctgggtatgg tattacctga agcgaaaaaa
     4081 acgatacgcc agcttgcagg tatccaatac ttttgt
//
LOCUS       DAOSGL010000206         4113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_19695_length_4113_cov_4.170281, whole genome shotgun sequence.
ACCESSION   DAOSGL010000206 DAOSGL010000000
VERSION     DAOSGL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19695_length_4113_cov_4.170281"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /locus_tag="PKY68_06775"
     CDS             complement(<1..2231)
                     /locus_tag="PKY68_06775"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH24775.1"
                     /translation="MRFILTSFALLFFTLNVFSQYSIQGRLLDKENDGPIEMATISLL
                     SAKDSSLISGAVSDYAGRYSFSKVAKGDYLISYKYLGYKQVIVQLKIEDKSQILKNVY
                     LEEEVTNLDEVEVRGMAAQMIIKGDTTEYNAAAFKMQENAVVEDLFKRLPGFMIESDG
                     SIMVNGERIGRIRVDGRRFFDGDMEMITKNFTADMVDKIQVFDELSEMAQLTGIEDEN
                     TQRIINITLKPDRRKGVFGNVSGGLGADFDKGLRYDENFFKDDFRYDANAMVNLFKEN
                     FRTTVTTGANNTNSSRSGRGGVGRGGSGITSTQNIGLNINAEPKTGMQLGGSTSYNHS
                     SNYGNTESFRESWLRDVTNTNNSKSSSNSHSNNAHLGFEMRWQIDSLNTLVIQPNASY
                     GLNSSNNRREFDYYTNGDSTSWGDSKNNNMSDNQNASLNLLFSHRSGRKRGRVLTFNV
                     NGGISENESLGFNESNKYTPDTAILLDQKSINTSNSYNLGLRGSLVEPLWNVQNFLEF
                     NASVNYSNRNSFRNQYDKDENGDYTLYNAEYSNEFWNTGLSQNMAMNYNYRDEKLNIV
                     AGVTASPSQTFSYTEYGNGEVRDIENKVFNYSPNLSVRYNIGGSRRHYVRVQYRGRSS
                     EPSVSQMQPVKNNTNLMHETVGNPGLLPAFSHSLNLNYSKSNPESLASFSTTLSAGMN
                     KDALVSNSIYDASGKEYRQTVNVDKAPFNANGSVTYNRPIIKNRLHFQTTTSAGMRQS
                     IGYS"
     gene            complement(2392..>4113)
                     /locus_tag="PKY68_06780"
     CDS             complement(2392..>4113)
                     /locus_tag="PKY68_06780"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH24776.1"
                     /translation="FEMEWKPDSFNTLIVQPSISYSLNQTDRYQDYDYYNNGDSTSWG
                     NSDNLSNSTNLNSRLNLIYSHRSKQKPGRSITVNLGGNYGESNSTGYNKSIKETPDSV
                     VNIDQKSLNTSGSYGLNMRTSYVEPLWNLQNLIEASVSFNYSGRDSEKEQFDNDGLGN
                     YPLENKDLEYSNSFKNYNFTQVAELNYRYVSQVWNIMAGFRAEPAQTFSYTTYGDGTY
                     YEIPQSVLNFSPSLSVRYVLGERRNFIRMEYRGRTQEPSVTQMQPVKNNSDLMNEMVG
                     NAHLVPSYSQNLRLIMSKYNPETFASWNATLFGDITKDALVANSIYDLSGKQYNQTVN
                     ADRPPFNLNGRLMYNTPLIPNRLHFNTGTSAGYTERIGYSSRKVSDLDVNNLPLGARS
                     LTKIYRFSEDLSLTFTHDIIEIGARGSFSYNNSLNNLNNTEQTTLNWSGSGNVNLHLP
                     NSINVSTDISYSDRYGYANFDQTEILWNATIDKTIKNKLTVSLSVNDILRQRLNVVQN
                     YGDNNVSYNKYNTLPAYFLLSFSYKIQSFGGGRTSSDDSPMPQRMGRGMGGGYRGGMG
                     GPFPPGM"
BASE COUNT         1061 a          867 c          925 g         1260 t
ORIGIN      
        1 gaataaccga tgctttgtct cataccggcc gaagtggtgg tttgaaaatg cagcctgttt
       61 tttattatag gtctgttata agtgacactg ccattggcat taaagggggc tttgtccaca
      121 ttcaccgttt gcctgtattc tttgcctgag gcatcgtaga tactgttgga taccagggcg
      181 tctttgttca taccggcgct gagcgtggtg ctgaaagaag ccagtgattc gggattggat
      241 ttggaataat tgagattcag actatggctg aaagcgggga gcaagcctgg atttcctacg
      301 gtttcgtgca tcaggttggt attgtttttg accggttgca tttgcgatac gctcggttcc
      361 gaagaacggc ccctgtattg tactctgaca taatgtctgc gggaaccgcc gatgttgtaa
      421 cgtacgctta gattgggtga ataattaaag actttgtttt cgatatccct tacttctcca
      481 ttaccgtatt cggtatagct gaaagtttga gaaggagagg ccgtgacacc ggcaacaata
      541 ttcaatttct catcccggta attgtaattc atagccatat tttgcgacaa accggtgttc
      601 cagaattcat tgctgtattc tgcgttatac agggtataat ctccgttttc gtctttgtcg
      661 tattgatttc tgaatgaatt tcgattggaa taatttaccg aagcgttgaa ttctaaaaaa
      721 ttctgtacat tccacaaggg ttctaccaac gaacccctca atcccagatt gtacgaattg
      781 gaagtgttga tcgatttctg atccaacaaa atagccgtat cgggggtata tttgttggat
      841 tcattgaatc ccaggctctc attttcgctt atcccgccat tgacattgaa agtaagcacc
      901 cggcctctct tccggccaga acgatggcta aacaacaggt tcaatgaagc attctgatta
      961 tcggacatat tgttgttttt ggaatcaccc caactggtgc tgtcgccgtt ggtgtaataa
     1021 tcaaattctc tccggttgtt gctggaattc agcccatagg aagcattggg ctgaatgact
     1081 aaagtattca gagagtcgat ttgccacctc atttcaaagc ccagatgagc gttattgctg
     1141 tgagagttgg aactcgattt agaattgttt gtgttggtga cgtcccttag ccaactttcg
     1201 cgaaaagact ctgtgtttcc gtaattagaa gaatggttgt aactggtgct tcctcccagt
     1261 tgcattcctg ttttgggttc ggcattaata ttcaggccga tattttgtgt tgatgttata
     1321 ccgctgcctc cccggccgac acctccacgt ccactacggc tggagttggt gttgttggct
     1381 cccgtagtaa ccgtagtacg gaaattctct ttgaacagat taaccatagc gttggcatcg
     1441 taacggaaat cgtctttaaa gaagttttca tcgtaacgca aacccttgtc aaaatcggct
     1501 cccaggcctc cggatacatt gccgaaaacg cccttgcgtc tgtcgggctt taaggtgatg
     1561 ttgatgatac gttgtgtgtt ctcgtcttcg atgcctgtta attgagccat ttcggacaat
     1621 tcgtcgaata cctgtatttt atcaaccata tctgccgtga aatttttggt gatcatttcc
     1681 atgtctccgt caaagaaacg tcttccatcg acacgaatac ggcctatacg ttctccgttg
     1741 accataatac tgccgtcgga ttctatcata aagcccggca gacgcttaaa caaatcctcg
     1801 accacggcat tttcctgcat tttaaaggcg gcagcattgt attccgtggt gtcgcctttg
     1861 ataatcattt gggcagccat tcctcgaact tctacttcat ccaaattggt gacttcttct
     1921 tcgagataga cgtttttcag tatttgcgat ttgtcttcta ttttgagttg aactatgact
     1981 tgcttgtagc ccaaatattt ataggaaatc aaataatctc ctttagctac cttcgaaaaa
     2041 gaatagcgtc cggcataatc actcacggct ccgcttatca gactggaatc cttggccgat
     2101 agaagactga tggtggccat ctctatgggc ccatcgtttt ccttatcgag caatctgcct
     2161 tgtatactgt attgggaaaa tacattcaga gtgaaaaata atagggcaaa cgaagtaaga
     2221 ataaacctca tgggaaaaca gttaggttat caacgaatgc ttagatgcac aggcaaagag
     2281 atagtttaat cccgaaatta acaaacaatg aaaataaatg ccaacaaata caaaagagaa
     2341 atgcctctca acacagggag gcatttccaa aacctaaatc aaaacctaaa attacatgcc
     2401 tgggggaaat ggaccaccca tgcctcctct atacccgccg cccatgccac ggcccatgcg
     2461 ttgaggcatg ggtgagtcat cgctactggt tctgcctcct ccgaaagact gaatcttgta
     2521 actgaaactc agcagaaaat aagcgggtaa tgtattgtat ttattgtagg aaacgttgtt
     2581 gtctccataa ttttgcacaa cgttgagtct ctggcgtaaa atatcgttta cgctgagcga
     2641 cacggttaat ttgtttttga tggttttgtc tatggttgca ttccacaaaa tttcggtctg
     2701 gtcgaaattg gcgtaaccgt aacgatcgct gtaactgata tcggtcgaaa cattgatgct
     2761 gttgggtaaa tgtaaattca cattaccgct gcccgaccaa ttaagagtgg tttgttcggt
     2821 attattcaag ttattcaggg aattgttata actaaaagaa cccctggcac ctatctctat
     2881 aatatcgtgg gtaaaagtca aagacaaatc ttcgctgaat ctgtaaatct tggtaaggct
     2941 gcgggctcca agcggcagat tattaacatc cagatcgctc acttttctgc ttgaataacc
     3001 gattctttcc gtgtaaccgg ccgaggtgcc ggtgttaaaa tgcagccggt taggtatcag
     3061 gggagtatta tacatcaatc ttccgttcag gttgaaaggg ggacggtcgg cattaacagt
     3121 ctggttgtat tgttttccgc tgagatcgta aatactgttg gctaccaaag catccttggt
     3181 gatatcacca aacaatgtgg cattccacga agcgaaagtt tcgggattgt atttggacat
     3241 aatcaacctc aggttttgac tgtacgaggg gactaaatgg gcgttaccca ccatttcgtt
     3301 catcaggtcc gagttgtttt tgaccggttg catctgggtc accgaaggct cctgggtgcg
     3361 gccacgatat tccatacgta taaaatttct gcgttctccc aatacatagc gtacactcaa
     3421 agaaggagaa aaattcaaca cgctttgcgg aatttcgtaa taggtgccgt ctccgtaagt
     3481 ggtataacta aaggtttggg cgggttctgc tctgaaaccg gccataatat tccatacctg
     3541 gctgacgtaa cggtaattca attcggctac ttgggtaaag ttgtaatttt tgaaagagtt
     3601 gctgtattcc aggtccttgt tttcaagagg atagtttcca agtccatcgt tgtcgaattg
     3661 ttctttttcg gaatccctgc cggaataatt gaacgaaacg gaggcttcga taagattctg
     3721 caaattccac aaaggttcta cataagacgt tcgcatgttc aggccgtaag aaccggaggt
     3781 gttgaggctc ttttggtcga tattaacaac agaatcgggt gtttctttga tcgatttgtt
     3841 gtaaccggta ctattggatt ctccgtagtt tccacccaaa tttacggtaa tggagcgacc
     3901 aggtttttgc tttgatctat gactataaat caaatttaac cgggaattta ggttcgttga
     3961 attgctcaaa ttatccgaat taccccaact ggtgctgtcg ccgttgttgt aataatcgta
     4021 gtcctgatat ctgtctgttt gattcagact gtaggatatg gatggttgta cgatgagggt
     4081 attgaatgag tcgggtttcc attccatctc gaa
//
LOCUS       DAOSGL010000207         4078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_19927_length_4078_cov_3.366642, whole genome shotgun sequence.
ACCESSION   DAOSGL010000207 DAOSGL010000000
VERSION     DAOSGL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19927_length_4078_cov_3.366642"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKY68_06785"
     CDS             complement(<1..301)
                     /locus_tag="PKY68_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24777.1"
                     /translation="MKKFWRIFLIVLSLVLLSAGVSFLTVSKLSDRLVNLVKEQSINQ
                     QEQPVRLVKAEVVPAIETDFTQAAGQSVHAVVHIASKTMRSAGSSGPMDIIEYFFG"
     gene            598..942
                     /gene="rnpB"
                     /locus_tag="PKY68_06790"
     ncRNA           598..942
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKY68_06790"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1001..2344
                     /locus_tag="PKY68_06795"
     CDS             1001..2344
                     /locus_tag="PKY68_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOH24778.1"
                     /translation="METSPKNIRQRIRSFLSFISRDIWRISEHEVGGLRHRFYNIIRT
                     IILAGKGFSNNELVTKASSLTFFSLFAIVPLLGLILGISKGFGFQRLIEEQLIRALPG
                     HTEVLDFLFDLVESVLDVAGSGVIVGIGISFLILSVWAILNNIEISFNKIWQIPKSRS
                     FFRKIADYMAILFVIPVLLILSSGASIFLSAMMEKSSILSAVSPLVNFLMKLSPFLIS
                     WLLFTMLYILAPNTKVRFKNAAISGLIVGTAFQVFQIIYIGGQLWVNRYNAIYGSFAA
                     FPLLLLWLQLAWIMVLFGAEITFASQNIQNFFFEKEIKNISDRYSYFTILMITSIICK
                     RFENREAPLSALDISTMYRIPSRLTQRILSLLVEMKIINQTVSDEDKRSLTYQPAMDI
                     NQITVGMILKSVFNFGTEDFKMDIQGRFRRHWNIMLNIERELMAKGDQILIKDLL"
     gene            2384..2971
                     /locus_tag="PKY68_06800"
     CDS             2384..2971
                     /locus_tag="PKY68_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOH24779.1"
                     /translation="MAKSRTTWIILIIVVLIGLWLIRIYNSLVTADENVSTAWSQVEN
                     VYQRRLDLIPNLVNTVQGAADFERNTLNEVIQARAAASSINLDAANLNEANLAAFQQA
                     QDGLGQALSRLMVVVERYPELKANQNFRDLQSQLEGTENRIAVERNRFNEAVRIYNTS
                     IRRFPKNVIAGIFGFEARAYFQAEENAQKAPQVQF"
     gene            2975..3769
                     /locus_tag="PKY68_06805"
     CDS             2975..3769
                     /locus_tag="PKY68_06805"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOH24780.1"
                     /translation="MKHLLRGSLLVLTFWLALCVQAQIPQRPVPPRLVNDFAGLLNPA
                     ESHYLERSLVAFDDSTSNQICLVIVNDLQGMDVLDLAFKIGEQWQVGQKGFDNGLVLL
                     IKTKTDTRGEVAIATGYGLEAVLTDAVCKRIIEEEILPQFRQDRYFEGIQAGLDRIKL
                     IASGEYSYSSSEGDGAAVFAFFVLFVVFIIVFLALVGKKHKGKNGGSDGNKGKGGLSD
                     LSKAIILSSMLGGRNRGGSWGGGSSSGGFGGFGGGRFGGGGAKGGW"
     gene            complement(3777..>4078)
                     /locus_tag="PKY68_06810"
     CDS             complement(3777..>4078)
                     /locus_tag="PKY68_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOH24781.1"
                     /translation="EIIEREEGGWLVDGQTSFYDFLAHFDLEDYYSEFNYNTISGLIL
                     SLLEHIPKEGERLHWKGFGFEIVDMDGARIDKIIVKQESESVLALEEPNEKNKED"
BASE COUNT         1152 a          895 c          890 g         1141 t
ORIGIN      
        1 atccaaaaaa atattcaata atatccatag gcccgctact gccggcagac ctcatcgttt
       61 tagaagcaat atgaacaaca gcgtgtacag attggcctgc agcttgagta aaatccgttt
      121 ctatggcagg cacgacttcg gccttaacca aacggacggg ttgttcctgt tgattgatac
      181 tttgttcttt gaccaggttt accaaacgat cagataattt ggaaaccgtt aaaaacgaaa
      241 ctccggcact cagcagcacc agagataaaa cgattaaaaa aattctccaa aactttttca
      301 ttgctgtata atttagtatt cagtttaata ttgatgtaac tttgttatgt atagctacaa
      361 aaacaattcc taaacgactg aaacaacttt ttatttcatc ccaattaaca gtatatcgct
      421 ctaattaata atgattaatt gcttctttaa caagatttta agattaatgt attcccctta
      481 aaggcagtta acgacaatcg tacaaataca gacattttgt cagtacaaaa ccagctgata
      541 gtaaaaaaag ggacaagatg gtacaaataa caaaatatgc actatttttg cacccaagca
      601 gagaaccggc ttgtcgcttt tataaagagg aaagtccggg caacgcagag caccatactt
      661 tctaacagaa agctgtttgt gaaagcagag ttaaggtaac agaaaataac cgcctccccc
      721 gggaggtaag ggtgaaaagg cggggtaaga gcccaccgct tcttcagtga tgatgaaggt
      781 tgtaccactt atgggttgca aggccaagta taccggcgat tcagggttgc tcgcccgatg
      841 tcggagggta ggccgctgga actttgtggc aacacaaggt caagataaat gacaagcgct
      901 tagtaataag cacagaaccc ggcttacagg ttctctgttt ttttagactt tcgaatatgg
      961 ttacatatat caccctttaa attattgacc acaaaggctc atggaaacat ccccaaaaaa
     1021 tatccgccaa cggatccgga gttttttaag cttcatctcc agagacatct ggcgaatttc
     1081 agaacacgaa gtcggcggat tgagacatcg cttctacaat attataagaa cgattattct
     1141 tgccggtaaa ggtttttcca ataatgagct ggtaaccaaa gcttcttccc ttaccttctt
     1201 ttcgctcttt gccatagtgc ctcttttggg tctgattttg ggtatttcaa aaggttttgg
     1261 ctttcaacgg cttatcgaag aacaactgat cagagctcta cccggtcata cagaggtcct
     1321 ggacttttta ttcgaccttg tagaatcagt actggatgta gcaggaagcg gagtcatagt
     1381 cggtataggt atttcgtttc tgattttgtc tgtttgggct attctcaaca acattgaaat
     1441 ctcgtttaat aaaatttggc aaataccgaa aagcagaagc ttcttccgta agattgcaga
     1501 ctatatggcc atattgtttg tcattccagt cttattgata ctatcgagcg gcgcttctat
     1561 ctttctcagt gctatgatgg agaaatccag cattttgtcg gctgtcagtc ccctggtgaa
     1621 ttttttaatg aaattatccc cctttcttat ctcctggttg ttgtttacta tgctgtacat
     1681 cctggcgccc aacaccaaag tgcgctttaa aaatgccgct atttcgggcc tgattgtggg
     1741 aactgctttc caggttttcc aaattatata cataggcggt cagttgtggg tgaaccgtta
     1801 caatgccatc tacggcagtt tcgctgcttt tcctcttttg ctgttatggt tgcagctggc
     1861 atggattatg gttctttttg gagccgaaat cacttttgcc tcccaaaaca tacagaactt
     1921 ctttttcgaa aaggaaatca aaaacatcag cgaccgctac agttatttca ctattctcat
     1981 gatcacaagc atcatatgta aacgcttcga aaacagagag gctccccttt cggccctgga
     2041 tatttccacc atgtaccgta ttccttcgag gcttacgcaa cgaattctga gtctgttggt
     2101 tgaaatgaaa attatcaatc aaaccgtcag tgacgaagat aaaaggtcgc ttacctacca
     2161 accggccatg gatattaatc agatcacagt gggcatgatt ctcaaatcgg tgtttaattt
     2221 tgggaccgaa gatttcaaaa tggacattca gggacgtttc cgccggcatt ggaatattat
     2281 gctgaacata gagagggaat taatggctaa gggagatcag atattaatca aagatttatt
     2341 ataaactcaa tcaactaaca ctattcactt aaattacact tctatggcaa aatcaagaac
     2401 tacttggatc attctaataa ttgtagtcct tattggactg tggctgatca ggatatacaa
     2461 cagcctggta acggcagacg aaaatgtttc tactgcctgg tcacaggtgg agaatgtgta
     2521 tcaacgccgg ttggacttga ttcccaactt ggtgaacacc gtgcagggag cagccgattt
     2581 cgagcgcaat accctgaacg aagttattca ggcccgtgcc gcagcctctt cgatcaatct
     2641 tgacgccgct aacctgaacg aagccaatct ggcagctttc caacaagcac aagacggact
     2701 tggacaggcc ctgagccggc taatggtagt tgttgaacgc taccccgaac tcaaggccaa
     2761 tcagaacttc cgggatctgc aatcgcaact tgaaggcacc gaaaatcgta ttgccgttga
     2821 acgcaatcga ttcaacgaag cggtaagaat atacaacact tccataaggc gctttccaaa
     2881 aaatgtaatt gccggaatat tcggttttga ggccagagcc tatttccagg ccgaagagaa
     2941 tgcccagaaa gctcctcagg tacaatttta acttatgaaa catctgttgc gaggcagttt
     3001 gctggtcctg actttctggc tggcgctctg cgttcaggct caaattcccc aaaggcctgt
     3061 ccctccgagg ctggtcaacg attttgccgg cttgctgaac cctgccgaat cccattatct
     3121 ggaacgcagt ttggtggcgt ttgacgacag cacttccaac cagatttgcc tggtcatagt
     3181 aaacgatttg cagggaatgg atgtgctgga tctggccttt aaaatcggag aacaatggca
     3241 ggtaggccaa aaaggctttg acaacggact tgttttgctc atcaagacca aaacagatac
     3301 aagaggagaa gttgccatag ccaccggtta cggcctggaa gcggtgctta ccgatgccgt
     3361 ctgcaaacgc atcatcgaag aagaaatatt gccccaattt agacaggatc gctatttcga
     3421 aggaatacaa gccggcctgg accgtatcaa actcattgct tcgggagaat acagctattc
     3481 ttcctcagag ggagatggcg cagccgtttt tgcttttttt gttttatttg ttgtttttat
     3541 aattgttttc cttgcactgg taggcaaaaa acacaaaggg aagaacggtg gcagcgacgg
     3601 caacaaaggt aaaggcggcc tgagcgattt gtccaaagcc ataatactta gcagtatgtt
     3661 gggagggcgt aacagaggtg gttcctgggg tggaggcagc tcaagcggag gattcggagg
     3721 tttcggcggc gggcgcttcg gtggaggagg cgctaaaggc ggttggtgat aaaagtctag
     3781 tcttctttat ttttttcgtt tggctcctcg agggctaata ccgattcgct ctcttgtttt
     3841 acgatgattt tgtcgattct ggctccgtcc atatctacaa tttcaaagcc gaagcctttc
     3901 caatgaagcc tctctccttc tttgggaata tgctccaaca aacttaagat caaaccactg
     3961 atggtattgt aattgaactc cgaatagtag tcctccaaat caaaatgagc gagaaaatcg
     4021 taaaaggaag tctgcccgtc gaccaaccac cctccttctt cgcgttctat aatttcct
//
LOCUS       DAOSGL010000208         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_19944_length_4076_cov_3.306391, whole genome shotgun sequence.
ACCESSION   DAOSGL010000208 DAOSGL010000000
VERSION     DAOSGL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19944_length_4076_cov_3.306391"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PKY68_06815"
     CDS             complement(<1..1023)
                     /locus_tag="PKY68_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOH24782.1"
                     /translation="MEILIILLLIFMNGFLSMSEIALVSSRKSRLEIDAKKGNLAAKR
                     ILKMSEKPERFLSTIQIGITLIGILTGLYSGEALSGDFARFLSKWPLLEPIASGLSKT
                     IIVIVVTYVTLIFGELLPKRIGMSAAEKTSKIVVGPMELLSKLGAPFVWILSVSIRGL
                     MRMFHISAGEEGRVTEEEIKAIIQEGTEGGEIEEVEQDIVERVFTLGDRNVGSIMTHR
                     SELVWLDVSRDNSYLSDLIKQNLFTVYPVADGELDELLGVVFLKDLFLQVDRPDFNLR
                     ELVHPALYLPENLNVYRALEQIKQAYVKYGLVTDEFGEIQGIVTLKDIMTALIGSVPE
                     QDEEQEI"
     gene            complement(1071..1676)
                     /locus_tag="PKY68_06820"
     CDS             complement(1071..1676)
                     /locus_tag="PKY68_06820"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOH24783.1"
                     /translation="MFSGIVEEAASVVRLKKEGGNLHLTLTCSFVKELSIDQSISHNG
                     VCLTVVDIKDQEYTVTAIQETLDKTNLGLLKPGDKVNLERSLLVNGRLDGHIVQGHVD
                     QTAVCTLVEETDGSWRYTFEYDFDKAMALQGYMTVEKGSVCVNGVSLTVCDSRDNAFS
                     VAIIPYTYEHTNFHRIKAGTVVNIEFDILGKYLSRMMHFAD"
     gene            complement(1826..1901)
                     /locus_tag="PKY68_06825"
     tRNA            complement(1826..1901)
                     /locus_tag="PKY68_06825"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1864..1866),aa:His,seq:gtg)
     gene            complement(1976..>2359)
                     /locus_tag="PKY68_06830"
     CDS             complement(1976..>2359)
                     /locus_tag="PKY68_06830"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HOH24784.1"
                     /translation="GEAFPLELNYMSTHPQHYPALKDNPALTEEMATIEARLNKASLS
                     YIPKTQLAKYMEDIPQGSIIAITTDRPGLDIAHIGFACRQNGRLYLLHASSSNSRVEI
                     SEQSLLTYLNGVKHFSGIMIAQVNK"
     assembly_gap    2360..2459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2460..2687)
                     /locus_tag="PKY68_06835"
     CDS             complement(<2460..2687)
                     /locus_tag="PKY68_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24785.1"
                     /translation="MNRKQFVHPIVTGLLSLLFVLSLSCKAKNTAVAFISGEDKAILS
                     AFFHQAREQRLTEMSSGQRIIAIASYFMGSPY"
     gene            complement(2708..3589)
                     /gene="folD"
                     /locus_tag="PKY68_06840"
     CDS             complement(2708..3589)
                     /gene="folD"
                     /locus_tag="PKY68_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOH24786.1"
                     /translation="MQLIDGKAVSEQIKKEIAQEVSLMKAAGKKVPHLAAVLVGHDGG
                     SETYVANKIKACQVCGFESSLLRFEETVSEETLLAAIDKLNKDQAVDGYIVQLPLPKH
                     IDEHKIIEAIDYRKDVDGFHPINVGRSFIGLPAFLSATPAGILELIKRYNIPTAGKHV
                     VVIGRSNIVGKPLAALLMRKDYPGNATVTVVHSYSSDIAEQCRKADIIVCALGRPGFL
                     KADMVKEGAVVIDVGTTRLPSDKTKSGFKLFGDVDFEAVAPKCSFITPVPGGVGPMTI
                     VSLMKNTLLAGSNAIYR"
     gene            complement(3607..>4076)
                     /locus_tag="PKY68_06845"
     CDS             complement(3607..>4076)
                     /locus_tag="PKY68_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOH24787.1"
                     /translation="KRMADRILGMGDVVSLVEKAQEQYDEMEARRLHKKIIKNQFDFD
                     DFRAQITQIKKMGNLKDLASMIPGVGKAIKNVDIDDNAFKNIEAIIDSMTPFERSNPA
                     LIDSSRRQRIARGSGTNIQEVNNLMKRFDETRKMMKTVSTMNTKKRHGGKKRK"
BASE COUNT         1093 a          959 c          833 g         1091 t
ORIGIN      
        1 aatttcctgt tcttcatctt gttccggcac tgaacctatt aaggcggtca taatatcctt
       61 cagggttaca ataccctgaa tttcaccaaa ttcgtcggta accagtccgt acttaacata
      121 agcttgtttt atttgttcca aggctctgta tacattcaga ttttcgggca aatacaaggc
      181 agggtgaacg agctccctca aattgaaatc cggccggtca acctgaagaa aaagatcttt
      241 aagaaaaacc actcccagca gttcgtcgag ttcaccatca gccacaggat acacagtaaa
      301 caaattttgt ttaatcaggt cgctcaaata cgagttatcg cgggaaacat ccagccacac
      361 cagctcactg cggtgagtca tgatcgatcc cacattgcga tcccccaacg tgaacaccct
      421 ttccacgata tcctgttcta cctcttcgat ttctccccct tcggtcccct cctgaataat
      481 agccttgatt tcttcttcgg tcactctgcc ttcttcgccg gcactgatgt gaaacatacg
      541 catcaatccc cggatggaaa ccgataaaat ccaaacaaaa ggagctccta gtttggacag
      601 cagctccatg gggccgacaa caatcttgga tgttttttct gcagcgctca tacctatgcg
      661 cttaggcagt aactcgccaa atataagggt aacataggta actacaatca ctattattgt
      721 cttggaaagc cccgaagcaa taggctccaa taaaggccat ttagacaaaa aacgggcaaa
      781 atcaccggac agggcctcac cggaatacaa accggtcaaa atgcctatca gtgtaatgcc
      841 tatttgaatg gtcgacagga aacgttccgg cttttccgac attttaagaa tacgcttcgc
      901 tgccagattg ccttttttgg cgtcaatttc caggcggctt ttgcgagaag aaaccaaggc
      961 gatttccgac atcgacagaa agccgttcat aaaaatcaac aataaaatga taagaatttc
     1021 cattatgtac aaaggagaat gtttttggta aaaaaccttc agcaggagaa tcaatctgca
     1081 aaatgcatca tgcggctcag gtatttgccc aggatatcaa attcgatatt cacaacagtt
     1141 cctgccttta tccggtggaa attggtgtgc tcgtaggtat agggaataat ggctaccgaa
     1201 aaagcattgt ccctggaatc gcaaacggta agactgactc cgttcacaca tacagaaccc
     1261 ttttcgacag tcatataacc ttgcaaggcc atagccttgt caaaatcgta ttcgaaagta
     1321 taacgccagc tcccatcggt ttcttcaacc aaagtacaaa cagcggtttg gtcaacatgt
     1381 ccctgcacaa tatgtccatc cagacgtccg tttaccagca ggctgcgttc cagattgacc
     1441 ttatccccag gtttgagtag gcccagattt gttttgtcca gggtttcttg tatcgccgtt
     1501 actgtgtact cctgatcctt aatatcaacc acagtaagac aaacaccgtt atgagaaata
     1561 ctttgatcta tgcttaattc cttcacaaaa gaacaagtaa gggtcaaatg cagatttcct
     1621 ccctcttttt ttaaacgtac tacagatgcg gcttcttcta caattccgga aaacatgttt
     1681 agagaattag ttgggcaaag atacttcatt atttcaattc ggcacaactt taggtaaaaa
     1741 atcaaaaaac agggcagata agtatcaatt ggatagtgaa gcagataaaa aaagtgcaaa
     1801 ttcctgaggg aatccgcact tttccgggtg gaagatgggg ctcgaaccca cgaccttcgg
     1861 aaccacaatc cgacgctcta accagctgag ctacgtccac catctatttt cggctgcaaa
     1921 agtagtaatt tttttgatat tttcttatgc caaccctgaa attctgcaat aaatatcact
     1981 tgttgacctg tgcaatcatg attccgctaa aatgtttaac accgttgagg taggtcaaca
     2041 gactttgttc cgatatttca acccgggaat tagacgaaga agcatgcaag agataaaggc
     2101 gcccattttg acggcaggca aaaccgatat gagcaatatc cagtcccggt ctgtcggtgg
     2161 taatcgctat aatggaacct tggggaatat cttccatata ttttgccaat tgtgttttag
     2221 ggatataact caggcttgct ttattcagac gagcctcaat ggtcgccatt tcttcggtca
     2281 gggcgggatt atccttcaga gccgggtaat gttgcggatg cgtagacata taattgagct
     2341 caagcggaaa agcttctccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2461 tacggactcc ccataaagta acttgcaatg gcaatgatcc tttgcccgct gctcatttct
     2521 gtcagccttt gttcacgggc ttgatgaaaa aaagcagata atatagcctt atcctcaccc
     2581 gaaataaatg caacagccgt attctttgct ttgcaagaca aactcaagac aaacaacaga
     2641 ctcagcaggc cggttacaat gggatgtaca aactgttttc ggttcattcg gacaaaagca
     2701 gtcaggttca acgataaatg gcattggaac ctgccaacaa ggtattcttc atcagtgaaa
     2761 ctatggtcat cgggcctaca cctcctggaa ccggagtaat aaaagagcat ttgggagcta
     2821 cagcctcaaa atcaacgtct ccgaagagct taaaaccgct tttagtttta tccgagggca
     2881 aacgggtagt tccaacatcg atcaccaccg ctccctcttt caccatatcg gccttaagaa
     2941 agccgggacg gcccagggca caaacaataa tgtcggcttt acggcattgt tcggcgatat
     3001 cggaagaata actatgcacc accgttaccg tggcattgcc cggatagtct ttgcgcataa
     3061 gcaaagccgc cagaggctta ccgacaatgt tactgcggcc tattaccact acgtgttttc
     3121 cggcagtggg gatattgtat cgtttgatca attcgagaat accggcgggg gttgccgaca
     3181 aaaaagccgg cagtccgata aaagaacggc ctacgttaat gggatgaaaa ccatccacgt
     3241 ctttgcgata atcgatcgct tcgatgattt tgtgttcatc gatatgtttc ggcaggggta
     3301 attgtacaat atagccatcc accgcctgat ccttgttgag tttatcgatg gctgccagta
     3361 atgtctcctc gctgaccgtt tcctcgaagc gcagcaagga ggattcaaaa ccgcaaacct
     3421 gacaagcctt gattttattg gccacataag tctcgctgcc tccgtcgtgg cctaccaata
     3481 cggcagccag atgaggcacc ttcttccctg cggctttcat aagcgatact tcttgggcga
     3541 tttctttttt tatctgttca gaaacagctt ttccgtcaat aagttgcata aataggtatg
     3601 gttgaattat ttgcgttttt taccgccatg cctctttttg gtgttcattg tgctgacggt
     3661 tttcatcatt ttacgggttt catcaaaacg tttcatcagg ttgttgactt cctgaatatt
     3721 ggtgccacta ccacgcgcta tgcgctgccg gcggctgctg tcgattaatg cgggattact
     3781 gcgttcaaag ggagtcatgg aatcgataat ggcttcgatg ttcttgaagg cattgtcgtc
     3841 gatatccaca ttcttgatgg cttttcccac accgggtatc atggatgcca ggtctttgag
     3901 attgcccatt tttttgatct gggtaatctg ggctctgaaa tcgtcgaagt caaactgatt
     3961 tttgatgatc ttcttgtgca ggcggcgagc ttccatttcg tcgtattgct cctgcgcttt
     4021 ttccaccaac gaaaccacat cgcccatgcc caaaatacga tcggccatac gcttgg
//
LOCUS       DAOSGL010000209         4066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_20018_length_4066_cov_3.369733, whole genome shotgun sequence.
ACCESSION   DAOSGL010000209 DAOSGL010000000
VERSION     DAOSGL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20018_length_4066_cov_3.369733"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PKY68_06850"
     CDS             complement(<1..884)
                     /locus_tag="PKY68_06850"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH24788.1"
                     /translation="MEKKTFPNGMDFYHKKTDAEGLLKLDLILPAGIQYQKRPLLARL
                     TNALLKEGSRSMTSAQIAERFDYYGAFILLGCGLENAYVNLYTPKKFFSFTLKLLNEL
                     LRYPSFPQKELDISLEKNLQRHLIELQKVQVLAGLEINKCLFGPEHPYAKQLQEEDFD
                     SFDSSLLKEFHRNHYSAHKCIALLSGEVNEEDGKLVEQVLGTEPWGASGCTPTNEAPI
                     RPLKPQKVLVEKAGSLQTALHFSIPLKIDKHHNDFKKLKFVNTLLGGYFGSRLMSSLR
                     EEKGYTYGISSNINVHRK"
     gene            complement(914..1657)
                     /gene="kdsB"
                     /locus_tag="PKY68_06855"
     CDS             complement(914..1657)
                     /gene="kdsB"
                     /locus_tag="PKY68_06855"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOH24789.1"
                     /translation="MRFLGLIPARYASSRFPGKPLVQIKNKPMIQWVYEQTHPWVDEL
                     WVCTDDERIFKAVEAFGGKVIMTSSAHRSGTDRCAEASSRLGGDFEVVINIQGDEPFI
                     QGEQIQALKACFNKPETEIATLVKAFKKEDGLQALLNPNSPKVVLNSRGEAMYFSRSV
                     IPYLRNFPQSQWLEKNVFYKHIGIYAYRRTVLAKLARLEASHLEQQESLEQLRWLENG
                     YKIGTALTQVENQAIDTPEDLERLLKNLR"
     gene            complement(1654..3057)
                     /locus_tag="PKY68_06860"
     CDS             complement(1654..3057)
                     /locus_tag="PKY68_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOH24790.1"
                     /translation="MKAGNYLLLSFLLFGLLSLNAEDSLAVRKLVYQVDLRDNVNSFT
                     WMQVQKGFAQAEAEEASLILLDMNTYGGEVVYADSIRTRILNTETPVCVFINNNAASA
                     GALICIACDKIFMRSGAVIGASTVVDQSGEVAPDKYQSYMRGIIRATAEAQGKDTLIA
                     DGDTMYVWKRDPAIAEAMVDPDVEIPGVIEKGKVLTFTATEALQNKYCDAIVQSKEEI
                     LETHYGYGDYELKVFRPSLKDKISGRLMGTALRAILIMIIVAGIWFELQSPGIGFPSI
                     AAGIAAILYFAPLYLDGLVQYWEIILFIVGVILIFLEIFVIPGFGIAGILGILAVVLG
                     LSFALVDNTGLSFDFEGLDVLGEALLLVILSMLVAFIISLWLTSKIGVGKGVFRHLAL
                     ESVQEKEAGYVAVPTTDKSVIGATGLAATDLRPSGKVRIGDVLYDAVAEFGYIEKGKT
                     IKVTRLENAQFYVEELK"
     gene            complement(3064..>4066)
                     /locus_tag="PKY68_06865"
     CDS             complement(3064..>4066)
                     /locus_tag="PKY68_06865"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOH24791.1"
                     /translation="THGGSTFGHWTGANSPPYASWCSSYDYDAPINEAGQTTEKFYAV
                     RELLAKYSDKALPKVPKALPMIEIPPFELNQIAPLFQNLPEPILSDQVHSMEFFDQGY
                     GSILYRTTLPAGAAGRKLIVEEAHDWAIVFVDGKRLGTLDRRKAEKILELPATEKEAV
                     LDILVENMGRVNYSKTIHDYKGITQRVSVQEGRKQKELTGFQVYRFPVEDALSGRLQF
                     VERSQDCHMPAYYKGSFNLDKTGDVYIDMQNWSKGLVWINGYAIGRFWEIGPQQTLFV
                     PGCWLNKGQNEIIVLDLQSPLEPVISGITTPILDKLLSDEETLERNKNANPDESGYF"
BASE COUNT         1023 a         1029 c          926 g         1088 t
ORIGIN      
        1 cttttccggt gtacgttgat gttggaagaa atgccgtagg tataaccctt ttcttcgcgc
       61 aggctgctca tcaatctgct gccgaaataa ccgcccagga gagtatttac aaatttcagt
      121 tttttaaaat cgttgtggtg tttatctatt ttcaatggga tggaaaagtg cagggcagtc
      181 tgcaaactac cggctttttc caccagcact ttttgaggct taagaggccg gataggcgcc
      241 tcattggtcg gagtacagcc ggacgctccc cagggttcgg tgcccaggac ttgttccacc
      301 agtttaccgt cttcttcgtt tacttctccg ctaagcaagg caatacattt gtgcgcagaa
      361 taatggttgc gatgaaactc tttgagcagc gaagagtcga aactgtcgaa atcttcctcc
      421 tgcaattgtt ttgcgtaggg atgttccggc ccgaacaaac atttatttat ttccaaaccg
      481 gccagtacct gcactttttg cagctctatc agatgtcgct ggaggttttt ttccaggctg
      541 atgtccaatt ccttctgggg aaatgaagga taacgcaaga gttcgttgag cagtttcaag
      601 gtgaaactga aaaacttttt aggtgtataa agattgacgt aagcattttc caaaccgcaa
      661 cccaaaagga taaaagcacc gtaataatca aagcgctctg caatctgagc cgaagtcata
      721 ctgcggctgc cctctttgag aagagcattt gtcaacctgg ccagcagagg acgcttttgg
      781 tattgtatgc ccgcaggtaa aatcaaatcg agtttaagca aaccttcggc atcggttttc
      841 ttatggtaaa agtccatccc gttcggaaaa gtcttttttt ccatgcgcgg gatgctaaag
      901 tcgtccggct tgttcatctt agatttttca ataagcgctc caaatcttcg ggcgtatcta
      961 tggcctggtt ttcgacctga gtcagggctg taccgatttt gtatccgttt tcgagccaac
     1021 gcaactgttc cagcgattcc tgttgctcca ggtgagaggc ttcgagcctg gccagtttgg
     1081 ccaatacagt tcttctatag gcataaatgc ctatgtgttt gtaaaagaca ttcttttcaa
     1141 gccattgact ttggggaaaa ttccgaaggt aagggataac cgaacggctg aagtacatag
     1201 cctctccccg ggaattcaga accaccttgg gtgaattggg attcaggaga gcctgcaaac
     1261 catcttcttt tttaaaagcc ttaaccaaag tggctatttc ggtttcgggc ttattgaaac
     1321 aggcctttaa ggcttgtatt tgttcccctt gaataaaagg ctcatcgccc tgtatgttta
     1381 ttaccacctc aaaatcacct cccagcctgg acgaggcttc cgcacaacgg tcggtaccgc
     1441 tgcggtgagc agaagaagtc atgatgactt taccgccaaa agcctcaacg gctttaaaaa
     1501 tacgttcgtc gtccgtacaa acccaaagct catctaccca gggatgagtc tgttcataca
     1561 cccactgtat catgggtttg tttttaattt gtaccagggg cttgcctgga aagcggctcg
     1621 aagcatagcg ggccggaatc aatcctaaaa atctcacttc agttcctcca cgtaaaattg
     1681 agcgttttct aaccgggtta ccttgatcgt tttccccttt tcgatgtaac cgaattcagc
     1741 gacggcatcg tacaacacat ctccgatacg gactttgccc gaaggcctca ggtcggtggc
     1801 ggccaagcct gtagcaccga tgacgctttt atctgtggtg ggtactgcca cataaccggc
     1861 ctctttttcc tgcaccgatt cgagggccag atgcctgaaa acacccttgc ccacaccgat
     1921 tttggaggtc aaccaaagcg aaataataaa agcaaccagc atgctgagta ttaccagcag
     1981 caaggcttcg cccaagacat ccagcccttc gaaatcaaaa ctcaggcctg tgttgtcgac
     2041 cagggcaaaa ctcaaaccca acaccaccgc caatatgcct aaaatgccgg caataccgaa
     2101 accgggaatc acaaagattt ccaaaaagat cagtatcacc ccgactataa acagtataat
     2161 ttcccaatac tgcaccagac cgtccaggta caggggggca aaatataaaa tggcggcaat
     2221 tccggccgca atgctgggaa aaccaatacc gggcgattgc agctcaaacc agatgcccgc
     2281 cacaatgatc ataatgagta tcgcccgtag ggcagtcccc atcagacgac cggatatttt
     2341 gtctttgaga ctgggcctga acactttcag ttcgtaatcg ccatagccgt aatgtgtctc
     2401 cagaatttcc tctttgcttt gaactatggc gtcgcaatac ttattttgca aggcttcggt
     2461 agccgtaaaa gtaagtacct tgcctttctc gatcacaccg ggaatttcca cgtcgggatc
     2521 taccatagcc tcggcaatcg ccggatcgcg tttccatacg tacatggtat cgccgtcggc
     2581 tataagagtg tccttaccct gagcttcggc tgttgccctg atgatgccgc gcatgtagga
     2641 ctgatatttg tccggcgcca cttctccgct ctgatccact accgtcgaag cccctataac
     2701 ggctccgctg cgcataaaga ttttatcgca ggcaatgcag atcagagctc cggccgatgc
     2761 ggcgttgttg ttgataaaca cacataccgg agtttcggta tttaggatac gggtacgtat
     2821 ggaatcggca tatacaacct ctccaccata tgtattcata tccagcagaa tcagagacgc
     2881 ctcttcggcc tcggcctgag cgaaaccttt ttgtacctgc atccaggtaa agctgttgac
     2941 attatcgcgt aaatcaactt gataaaccag tttacgaacg gcaaggctgt cttctgcatt
     3001 taaactaagc aggccaaaaa gcagaaagct cagtaaaaga taattcccgg ctttcataag
     3061 aatttagaaa tagccactct cgtcgggatt ggcgtttttg ttgcgttcca gtgtttcttc
     3121 gtcggaaagc aacttatcca ggataggagt cgtgattccg ctaataacag gctcgagagg
     3181 actctgcaag tcgagcacta taatttcgtt ctgccctttg ttgagccaac aacccggcac
     3241 aaacaaggtc tgctgaggcc ctatttccca gaagcggcct atggcgtatc cgttgatcca
     3301 taccagacct ttgctccaat tttgcatatc gatgtaaaca tcgccggttt tgtccagatt
     3361 gaacgaacct ttgtaatagg cgggcatgtg acagtcttga gacctctcaa caaattgcaa
     3421 cctgccgctc aaagcgtctt caacaggaaa acggtacacc tgaaaacctg taagttcttt
     3481 ttgtttccgg ccttcctgca ccgaaactct ttgcgtaata cctttgtaat cgtgtatggt
     3541 tttagaataa ttcacccgac ccatattctc gaccagaatg tccaatacgg cttccttttc
     3601 ggttgccggc aattccagta ttttttcggc tttgcggcga tcgagagtgc ccaggcgctt
     3661 accgtccaca aaaacgatgg cccagtcgtg cgcctcttcc actataagtt tacggcctgc
     3721 agctcctgcc ggcaaggtag ttctgtacaa tatgctgccg tagccctgat cgaaaaattc
     3781 cattgaatgt acctgatccg acaggatggg ttcgggcaga ttttggaata agggcgctat
     3841 ctgattcagt tcaaacggcg gaatttctat catcggcaaa gctttgggca ctttgggcaa
     3901 agctttgtcg ctgtattttg ccaacaattc acgtacggca taaaatttct cggtggtctg
     3961 tccggcttcg ttgatgggtg catcgtaatc gtaggagctg caccacgaag cgtaaggagg
     4021 tgaattggca cccgtccagt ggccgaaggt gcttcctccg tgagtc
//
LOCUS       DAOSGL010000210         4064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_20033_length_4064_cov_2.810676, whole genome shotgun sequence.
ACCESSION   DAOSGL010000210 DAOSGL010000000
VERSION     DAOSGL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20033_length_4064_cov_2.810676"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /gene="ffh"
                     /locus_tag="PKY68_06870"
     CDS             complement(<1..1027)
                     /gene="ffh"
                     /locus_tag="PKY68_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOH24792.1"
                     /translation="MFENLTERLERSFKILKGQGRITEINVAETLKEVRKALLDADVN
                     YKTARQFTDNVKEKAMGMNVLTAVKPQEMMIKIVHDELTELMGGESVDLNIKGSPAII
                     LMSGLQGSGKTTFSCKLAKLLKSKRGKNPLLVACDVYRPAAIEQLKVLGEQIGVPVYT
                     EENQKDPVQIARNAVRFARQQGNDLLIIDTAGRLAVDELMMTEIAAIKQAVNPHEILF
                     VVDAMTGQDAVNTAKEFNDRLDFDGVVLTKLDGDTRGGAALSIRSVVQKPIKFIGTGE
                     KPEALDVFHPKRMADRILGMGDVVSLVEKAQEQYDEMEARRLHKKIIKNQFDFDDFRA
                     QITQIKKM"
     gene            complement(1037..2026)
                     /locus_tag="PKY68_06875"
     CDS             complement(1037..2026)
                     /locus_tag="PKY68_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH24793.1"
                     /translation="MKITLIGASGFVGTRLMDILRDKGHELLNIDKQQSHFFPDLTTI
                     ANVLDKQRMLELLKDTDLVVLLAAEHRDDVSPVSLYYDVNVNGMQHVLEAMEYNGVKR
                     LIFFSSVAIYGLNKQNPDESFAADPFNHYGKSKWQAEQLLQNWYEKHKDWNINVVRPT
                     VIFGERNRGNVYNLLSQISQGRFLMVGKGQNVKSMAYVGNVVAFVDFLIDTHKVAYQV
                     YNYVDKPDFDMNKLLALVGKVLGKRLSKVHFPYFLGMLGGWGFDLLSWLTGRKFSVSS
                     VRVRKFCATTQYDASKMLASGFNAPYTLEEGLSRTLEFEFVHPRTDDITFKSE"
     gene            complement(2033..2776)
                     /locus_tag="PKY68_06880"
     CDS             complement(2033..2776)
                     /locus_tag="PKY68_06880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH24794.1"
                     /translation="MISIITPCYNSVDFISQTIDSVIAQTYTDWEMIIVDDNSKDNSP
                     EIIQEYCRKDPRIKYIRTERPSGSPTLPRNIGLKHARGRFIAFLDSDDMWLPTKLEEQ
                     LKLFRENKVAIVYSNYEKISEEGKRGNRIIKAPAKADYKTLLKGNVMGCLTVMYDSEK
                     VGKRFFDSIGHEDYAVWLSILKEGYTAKNTNTVTALYRVRRQSVSSNKWDVLSWQWNI
                     YVKVEKTGIPKAIYYFINYAVRAFLKRIK"
     gene            complement(2773..>4064)
                     /locus_tag="PKY68_06885"
     CDS             complement(2773..>4064)
                     /locus_tag="PKY68_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH24795.1"
                     /translation="VDVQYVMDRIAAKNIEALVVDCSTPSIINDIQVVDRIVDKYPKL
                     PVLMVGRHVSVLPEESLKASRHLKYVAIKEYDYIVRDWLEALSSGASMSAVKGLVWKN
                     ASGEIVRNQPAELVLNLDELPFVSEVYKRFLRVEDYYYGFAPYPNITFDTSRGCPYHC
                     TFCAFPQTFSGHRMRYRSASNVADEFDYIKKEFPKVKSITFEDDTYIISKDHVLGIAD
                     ELIRRGNKIPYNVNQRADLIADLEFFKTLKKSGLRLVIVGFESGDDEVLKHMKKNLNL
                     AKAEEFMDLCRKAGILVHGCFMVGNLNETKETMQKTLDLAMKLQPDTAQFFPLMVYPG
                     TEAYQEAKDKGYLITEDWNQWLTETGLHNAVINLPNLTNEDLVEFCDYARRKYYTHPK
                     YLIRKMGQSLTDFDEFKRNWIGFKSLLKYLIKGSYSK"
BASE COUNT         1046 a          954 c          857 g         1207 t
ORIGIN      
        1 ccattttttt gatctgggta atctgggctc tgaaatcgtc aaagtcaaac tgatttttga
       61 tgatcttttt gtgcaggcgg cgggcttcca tttcgtcgta ttgctcctgc gctttttcca
      121 ccaacgaaac cacatcaccc atgcccaaaa tacgatcggc catacgctta ggatgaaaca
      181 catccagcgc ttcaggtttt tcacccgttc cgataaactt tatgggcttt tgtaccacac
      241 tccgaatgga caaggctgca ccaccccggg tatcgccgtc gagcttggtg agcaccactc
      301 cgtcaaaatc cagacgatcg ttgaattctt tggcggtgtt gacggcatct tggccggtca
      361 tggcatctac aacgaacagt atttcatggg gattgaccgc ttgtttgatg gccgcgattt
      421 cagtcatcat cagctcatcc acggcaagcc ggccggcggt atctataatc agaagatcgt
      481 ttccctgttg tctggcaaaa cgcacggcgt tacgggcaat ctgtaccgga tctttttggt
      541 tttcttccgt atacacgggc acaccgatct gctcgcccag tactttcaat tgttcaatgg
      601 cggccggacg gtacacgtca caggctacca aaaggggatt tttgcccctt ttacttttta
      661 acagtttggc cagcttacag gaaaaagttg ttttacccga accttgtaaa ccggacatca
      721 aaataatggc aggactgcct ttgatattta aatccacgct ttctccaccc atcaattcgg
      781 tgagctcgtc gtgaacaatc ttgatcatca tttcctgagg cttaacagcc gtcaggacgt
      841 tcatacccat ggccttttcc ttgacattgt cggtaaattg ccgggcagtc ttataattta
      901 cgtctgcatc caggagtgct ttgcgtactt ctttaagcgt ttcggccaca ttgatttcgg
      961 taatacggcc ttgtccttta agtattttga acgagcgttc cagccgttcg gtcaaattct
     1021 caaacatcat attgtattat tcgcttttaa acgtaatgtc gtcggttctg ggatgaacaa
     1081 attcaaattc gagcgttctc gataaacctt cttccagggt ataaggagca ttaaaacccg
     1141 aagccagcat tttggaggcg tcgtactgag tagttgcaca aaacttgcgt acacgcacgc
     1201 tactcacaga aaacttacgg cctgtcaacc acgacagcag atcgaagccc cagcctccga
     1261 gcatacccaa aaaataggga aaatgcactt tcgaaaggcg tttccccaac accttgccta
     1321 ccagagccaa taatttgttc atatcaaaat cgggcttatc gacgtaatta taaacctgat
     1381 aagccacttt atgtgtatca atcaaaaaat caacaaaggc aaccacattg cctacataag
     1441 ccattgattt aacattttgt cccttgccca ccatcaggaa acgtccctgc gaaatctgcg
     1501 acaacagatt gtaaacattg cctctgttgc gttcgccaaa aatcaccgtg ggacggacaa
     1561 cattgatgtt ccaatcctta tgtttttcgt accaattttg caataactgt tccgcctgcc
     1621 atttactttt gccgtaatga ttgaagggat cggcagcaaa gctttcatcg ggattttgtt
     1681 tattgagtcc gtagattgcc accgaactga aaaagatcag gcgtttgact ccgttatact
     1741 ccattgcttc gagtacgtgc tgcatcccgt tcacgttgac atcgtaatac aaagaaacag
     1801 gactcacgtc gtctcggtgt tcggcagcca gtaaaacaac cagatcggtg tctttgagta
     1861 attcgagcat cctttgcttg tccaacacgt tggctatggt ggtcaaatct gggaagaagt
     1921 gactttgttg cttgtcaata ttcaataatt cgtgaccttt atcacggaga atatccatta
     1981 aacgggttcc gacaaagccg gaggctccga tcaaggttat tttcatacca gcttatttta
     2041 ttcgttttaa aaaggccctg acggcataat ttataaaata atatatagcc ttgggtatac
     2101 ctgttttttc tactttaaca taaatattcc actgccagga gagcacatcc catttattgg
     2161 aagaaaccga ttggcgtctg acccggtaca atgccgttac cgtattggtg tttttggctg
     2221 tgtagccttc tttgagtata gacaaccata cggcataatc ttcgtgtccg attgaatcga
     2281 aaaagcgttt acctactttt tcggaatcgt acatcaccgt caaacaaccc atcacatttc
     2341 ccttaagcaa agttttgtag tctgctttgg cgggagcttt tataattcga ttgccccttt
     2401 tgccctcttc ggatattttt tcgtaattgg aataaacgat ggctacctta ttttcccgaa
     2461 aaagtttgag ctgttcttcg agtttagttg gcagccacat atcgtcactg tccaaaaagg
     2521 caataaatct tccacgggcg tgctttaaac cgatgtttcg gggtaaagtc ggtgagcccg
     2581 aagggcgttc cgtcctgatg tattttattc ttgggtcttt gcgacaatat tcctgaatta
     2641 tttccggcga attatctttt gagttgtcgt caacgataat catctcccag tctgtatatg
     2701 tttgagcaat aacagaatca atggtctgag aaataaaatc caccgaattg tagcagggag
     2761 taattattga aatcattttg aataagaccc tttaataaga tattttagta atgatttgaa
     2821 acctatccag ttacgtttga attcgtcaaa atccgtcaag gattgtccca ttttgcggat
     2881 caggtattta ggatgggtgt aatattttct tctggcgtaa tcgcaaaatt ccaccaggtc
     2941 ttcgttagta aggttgggca gattgattac cgcattgtgc aaaccggttt cggtcagcca
     3001 ttgattccag tcttccgtaa tcaaatagcc cttgtcttta gcctcctgat aggcctcggt
     3061 tccgggataa accatcaaag ggaaaaactg agccgtgtcg ggttgcagtt tcatggctaa
     3121 atcgagggtt ttttgcatgg tttccttagt ttcgttcagg ttgcccacca taaagcaacc
     3181 atgcaccaaa atacctgctt tgcgacaaag atccataaac tcttcggcct tggccaggtt
     3241 gaggttcttt ttcatatgct taagcacctc gtcatccccg ctttcgaagc cgacaataac
     3301 caacctgagc cccgattttt tgagcgtttt gaaaaactcc agatcggcaa tcaggtctgc
     3361 cctttggttt acgttatatg ggattttatt gccgcgtcgt attaattcat cggctatacc
     3421 aaggacatga tctttgctga tgatgtaggt atcgtcttcg aaagtgatgg actttacctt
     3481 gggaaattct ttctttatgt aatcgaactc gtcggccaca ttggaggccg agcggtaacg
     3541 catacgatgt ccgctgaaag tctgaggaaa agcacaaaag gtgcagtgat agggacaacc
     3601 acggctggtg tcgaaggtta tgttgggata cggggcaaaa ccgtagtaat aatcttcgac
     3661 tctcaaaaaa cgcttataga cttcagatac aaaaggtagt tcatccagat ttaaaaccag
     3721 ctctgcgggc tgattgcgca ctatttctcc gcttgcattt ttccacacca gacctttcac
     3781 ggcagacatg gaggctccgc tcgacaatgc ttccagccag tcgcgcacta tataatcgta
     3841 ttcttttatg gctacgtatt tgagatgtct ggaagcttta agcgattctt cgggtaaaac
     3901 ggacacatga cgacccacca ttagcacggg tagcttgggg tatttatcca caatccggtc
     3961 aaccacctgt atgtcgttta tgatactcgg ggtagagcaa tcaactacca gggcttctat
     4021 gtttttggca gcaattctgt ccatgacata ttgcacgtcg acac
//
LOCUS       DAOSGL010000211         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_20085_length_4055_cov_4.777500, whole genome shotgun sequence.
ACCESSION   DAOSGL010000211 DAOSGL010000000
VERSION     DAOSGL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20085_length_4055_cov_4.777500"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PKY68_06890"
     CDS             <1..1309
                     /locus_tag="PKY68_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH24796.1"
                     /translation="MNDTLFIVEVITGGPSEKVGLLAGDRILSVDDEVIAGVKMSNKE
                     VMKRLKGPKGTLVRIGVKRRGEPELLEFRIIRDKIPLYSLDAAFMADKKTGYIRLSRF
                     SATTMKEFKEAFEELRQQGMEQLILDLQSNGGGYMNTAVELADEFLPKGRSVVYTEGL
                     NSPRYQADASERGSFEKGNLIVLIDEFSASSSEIVAGAVQDWDRGLIIGRRSFGKGLV
                     QRIFPLGDGTALKLTVSRYYTPSGRCIQKPYREGVEAYGRDLIERYNRGEMSSADSIH
                     FPDSLRYSTMIEKRTVYGGGGIMPDVFVPVDTSRYTDLHRELVNKGILNRFILTYVDE
                     NRRALLDKYGENSQEFIQEFQFDDELLEELFKLAIKENVKITDEHRQEDLSVLRLQCK
                     AYMARDLWKSADYYKVMAPLNESLQEALRILGDKKLKARYGLL"
     gene            1323..2291
                     /gene="dusB"
                     /locus_tag="PKY68_06895"
     CDS             1323..2291
                     /gene="dusB"
                     /locus_tag="PKY68_06895"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOH24797.1"
                     /translation="MKIASLELPEKALLLAPMEDVTDSAFRLMCKQFGADMVYTEFVS
                     SDALIRNVSKTNQKLRISDEERPVAVQIYGKDPATMARAAQICEEAGPDILDINFGCP
                     VRKIASKGAGAGLLNNIPLMLEITRAVVDAVKIPVTVKTRLGWDEQSKIIVDLAERLQ
                     DCGIAALSIHGRTRSQMYTGKADWTLIAEVCNNPRMHIPIIGNGDIDSGEICLRRFNE
                     SGVAAVMIGRACIGRPWIFKEIKHYLATGTELELDNRWKMEVLKQQILNSIDRLDERR
                     GILHIRRHLAATPLFKGRPNFRETRIAMLRAEHLDELFRILDGIEL"
     gene            2288..3529
                     /locus_tag="PKY68_06900"
     CDS             2288..3529
                     /locus_tag="PKY68_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOH24798.1"
                     /translation="MKPELMAPVGSYDSLTAAAQGGADSVYFGVEALNMRSHSSQNFG
                     LEELEKIVAFCRAKNMKSYLTINVVLYDQDLILMRSLVDAAAQAGVDAIIASDVAVMQ
                     YAKLKGVEVHLSTQLNISNIEALRFYAQFADVVVLARELNLDQVKAIHQQIKAENICG
                     PGGQPVRIEMFCHGALCMAVSGKCYLSLHEMNASANRGACMQICRRAYRVQDVETGSD
                     IELTVDNQYILSPKDLKTIHFLNKMLDAGVEVFKIEGRARGPEYVHTVVSCYREALTA
                     CLDRTFSEDKIKNWDLRLARVFNRGFWDGYYLGQRLGEWSHRYGSSATTRKVRIGRGI
                     NYFSKLAVAEFQLESGELSLGDEILITGPTTGVIESRVEEIRLEDRPVEKAVKGQRIS
                     IPLASKIRPSDTLYKIVPFRP"
     gene            complement(3526..3720)
                     /locus_tag="PKY68_06905"
                     /pseudo
     CDS             complement(3526..3720)
                     /locus_tag="PKY68_06905"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
     gene            3793..>4055
                     /locus_tag="PKY68_06910"
     CDS             3793..>4055
                     /locus_tag="PKY68_06910"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOH24799.1"
                     /translation="MTEETALFEIRLKVRDYECDLQGIVNNANYQHYLEHARHEFLRS
                     IGLSFADFHYRGIDFVVTRVEIDYKTPLTSGDSFTVSLNIKKEG"
BASE COUNT         1111 a          921 c         1073 g          950 t
ORIGIN      
        1 gatgaacgac actcttttta tcgtggaggt gataacagga ggcccttccg aaaaagtagg
       61 ccttctggca ggcgatcgca tcctgagcgt ggacgatgag gtaattgccg gtgtaaaaat
      121 gtccaacaaa gaagttatga aacgtctcaa agggccgaaa ggcactttgg tgcgaatcgg
      181 ggtgaaaaga agaggagagc cggaactgct cgaattcagg ataattcgcg acaaaatacc
      241 gctttacagc ctagatgctg cttttatggc agataagaaa accgggtata tccgtctgag
      301 ccgtttcagc gccaccacca tgaaggaatt taaggaagct ttcgaagaac tccggcagca
      361 aggtatggaa caattgatac tcgatttgca gtccaacgga ggcggttaca tgaatacggc
      421 cgtggagctg gccgacgaat ttttacccaa aggccgcagc gtagtttata ccgaaggact
      481 taacagtccc cgttaccagg cggatgccag cgagcgaggc tcttttgaaa aaggcaacct
      541 tattgtgctt attgacgaat tttctgcctc atccagcgaa attgtggccg gtgccgtgca
      601 agattgggac agaggattga ttattggccg gcgcagtttc ggcaaaggac tggttcagcg
      661 cattttcccc cttggtgacg gcacggcgtt gaaattaacc gtatcgcgct attacacccc
      721 cagcgggcgt tgtatacaaa aaccttaccg ggaaggggtg gaagcttacg gacgcgattt
      781 gatagagcgt tataaccggg gcgagatgag cagtgccgac agtattcatt tccccgattc
      841 cctgcgctat tccactatga tcgaaaaacg tacggtttac ggcggaggag gtattatgcc
      901 cgatgttttc gtgcccgtcg ataccagccg ttacaccgat ctacacaggg aactggtcaa
      961 caaagggatt ttaaaccgct ttatcctgac ctatgtggac gaaaaccgtc gggctttgct
     1021 ggataaatac ggagaaaatt cgcaggaatt tatccaagag ttccaattcg atgacgaact
     1081 gctcgaagag ttgtttaaac tggccattaa agaaaacgta aaaatcaccg acgaacaccg
     1141 gcaggaagat ttatccgtgc tcaggctgca atgcaaagca tacatggctc gcgacctttg
     1201 gaagtcggca gattattaca aagtgatggc tcctctgaac gagtcgctgc aggaggcctt
     1261 gcgcattctg ggcgacaaaa agctaaaagc ccgctacggc ttgctatgaa gtttgtgaga
     1321 gaatgaaaat agcttcactt gaattgcccg agaaagccct cttacttgct cccatggagg
     1381 atgtcacaga ttcggctttc cgcctgatgt gtaagcaatt cggggcagac atggtttata
     1441 cggaatttgt ttcttccgat gccttgatcc gcaatgtgag caaaaccaat caaaaattac
     1501 gtatctctga cgaagaacgt cccgtagccg tacaaatcta tggcaaggat cccgccacca
     1561 tggcccgggc cgcccaaatt tgcgaagagg ccgggcccga catcctcgac attaattttg
     1621 gttgtccggt gcgtaagata gcaagcaaag gagccggagc cggattattg aacaacattc
     1681 ccctcatgct cgaaatcaca cgcgccgtgg tagatgccgt aaaaattccg gtgacagtca
     1741 aaacccgcct gggctgggac gagcaatcca aaataattgt cgatttggcc gagcgtctgc
     1801 aagattgcgg catcgcagcc ctcagtattc acggacgcac ccgcagccag atgtataccg
     1861 gcaaagccga ctggacgttg attgccgaag tctgcaacaa tccccggatg catattccca
     1921 tcatcggcaa cggcgacatt gattccggag aaatttgcct ccgccgtttc aacgaaagcg
     1981 gggttgccgc agtgatgata ggcagggcct gtataggacg tccctggata tttaaagaaa
     2041 tcaagcatta tctggccacc ggcacggagc ttgaattgga caatcgatgg aaaatggagg
     2101 tgctaaaaca gcaaattttg aacagtatag ataggctgga tgaacgcaga ggtattttgc
     2161 atattcgccg gcatttggca gccacccctt tatttaaagg acgtcccaat tttagagaaa
     2221 cacggatagc catgctaaga gccgaacacc ttgacgagct gttccgaata ctagacggta
     2281 tagaactatg aagcctgaat taatggcccc tgtgggctcc tacgattcgt tgactgccgc
     2341 tgcccagggc ggagccgatt cggtctactt cggtgtggag gctttgaata tgcgttcaca
     2401 ttcctcgcaa aatttcggat tggaagagtt ggaaaagatc gttgcctttt gcagagcaaa
     2461 gaacatgaag tcctacctca ccatcaacgt ggtgctgtac gaccaggact tgattttgat
     2521 gagatcgctg gtcgatgcgg ctgctcaggc aggggtagat gccattatcg cttcggacgt
     2581 ggccgtgatg caatacgcaa agctcaaggg cgtcgaagtg catttgtcca cccagctcaa
     2641 tatttcaaat atcgaagcgc tcaggtttta cgcccaattt gccgacgtag tggtgctggc
     2701 cagggaattg aatctggatc aggtgaaggc catacatcaa caaataaaag cggaaaatat
     2761 ttgcggcccg ggcgggcagc ctgtccgcat agaaatgttt tgccacgggg ctttgtgtat
     2821 ggccgtctcg ggtaaatgtt acctaagctt gcacgaaatg aacgcttcgg ccaacagggg
     2881 agcctgtatg caaatttgcc gccgggccta cagggtgcaa gacgtggaaa cgggcagcga
     2941 tatcgagctg acagtggaca atcagtatat tttgtcgccc aaggatttga agactattca
     3001 tttcctcaat aaaatgctgg atgccggcgt ggaggttttc aaaatagaag gcagagcccg
     3061 gggaccggaa tacgtgcaca cggtggtgag ttgttatcgc gaagctttga ccgcctgtct
     3121 cgaccggact tttagcgaag ataaaatcaa gaactgggat ctgaggctgg ccagagtgtt
     3181 caaccgggga ttctgggacg gttattattt ggggcagagg ctgggagagt ggtcacatcg
     3241 ctacggctct tcggcaacca cccgcaaagt acgtataggc cgtggtatca attatttttc
     3301 caaactggcc gtagccgagt ttcaattgga aagcggcgaa ttgagcttag gagatgaaat
     3361 tctgattacc ggccctacaa cgggcgttat cgaaagcagg gtagaggaaa taagactgga
     3421 agaccggccg gtggaaaaag cagtaaaggg ccagcggatt tccatccctc tggcaagcaa
     3481 gatcaggcca tcggatacct tatataagat agttccgttc aggccttagc tccggtcttc
     3541 ggggatcaga atcagcataa tgatatacag caagattccc gggaagccgg ctgtaaataa
     3601 acttaaggca cagtagagta ccctgaccaa aacgggatcg atattcagtt attctgcaag
     3661 ccctccaagt acgccgccta tcatgcggtc tttgcgtgaa cgtgttaatt tcctttccat
     3721 agggtaatgt aaattaagac aggtaaattt gtgcgcaaaa atatgtttta tatgtaaatc
     3781 aatctaataa ttatgacaga agaaaccgct cttttcgaaa ttcgcctcaa agtaagagat
     3841 tacgaatgtg atttgcaagg catcgtgaat aatgctaact atcagcacta tctggaacac
     3901 gccaggcatg aattcctgcg cagtataggt ctgagtttcg ccgattttca ttaccggggc
     3961 atcgattttg tggtgaccag agtggaaata gattataaga cgcctttgac cagcggagac
     4021 agttttacgg taagcttgaa cataaagaaa gaagg
//
LOCUS       DAOSGL010000212         4030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_20280_length_4030_cov_4.481761, whole genome shotgun sequence.
ACCESSION   DAOSGL010000212 DAOSGL010000000
VERSION     DAOSGL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20280_length_4030_cov_4.481761"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PKY68_06915"
     CDS             complement(<1..608)
                     /locus_tag="PKY68_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH24800.1"
                     /translation="MRNLLIVFLLFTVPLSAQTLTEARAMYINGEYEKALPVFEQELK
                     RKPKDVNLNYWYGACLYHTGNPTAALPYLINAEKSKIPAASLLLADYYHKQYQFDKSA
                     GHLDKYIAFGLSSNSAEVASMLKASEKAKQMLKAVEDVVFIDSLVVPMDSVFENIRLH
                     SANGTILPLRKLFPEKGDSLSVAYLTERKDCVFYDDTTAENG"
     gene            complement(797..3718)
                     /locus_tag="PKY68_06920"
     CDS             complement(797..3718)
                     /locus_tag="PKY68_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HOH24801.1"
                     /translation="MNTRRKTAALFFAAVLIAGTTLAANPARIKFNFNPSWLLHIGDQ
                     PGAHNPEFDDTEWKTVHLPAAFNEDEAFAKSIHDLTDTIVWYRKHFKIPSTYKNQKVF
                     IEFEGLRQAAEIWINGREIGLHENGVMAFGLDLTDHLVFGNKENVIALRIDNDWKYKE
                     KATGTGFQWNNNNFNANYGGLTKNVVLHICGKLYQTLPLYSHLGTVGTYVYATDINVR
                     DKTAVLNVESEVKNEQEKAQTFYMKVIVRDNDHNMIREFNSDEITLRPGESCIVKASG
                     NLKALRFWSWGYGYLYNITSSLISGKKTLDLVRTQTGIRKTAFRNGMFYLNERVLHLK
                     GYAQRSSNEWPAVGMSVPPWLSDFSNGLMERGNANLVRWMHVTPWKQDIESCDRVGLL
                     QAMPAGDAESDVEGRSWELRKELMRDAIIYNRNHPSIIFYECGNETISEEHMAEMKAI
                     RDIYDPNGGRAIGSREMLDSKEAEYGGEMLYINKSAGIPMFAHEYCRDEGLRKYWDNY
                     SYPYHRDGEGPLYRNEDASAYNRNQDSFAEEAVRRWHEFWVHRPGTGRRVSAGGVNII
                     FSDTNTHFRGAENYRRSGEVDAMRIEKEAYYAHKIMWDGWVEVEGNKYSNHIIGHWNY
                     EPGTVKDVLVVSNASAVELFLNGRNLGFGERQHHFLFKFRDVPFEAGSLKALSYDSRG
                     QLAGEPAEIKTTDEAAALRLQYWQHPKGFRADGSDMVLIQVEVVDKNGLRCPLANHLI
                     HFDIEGPAEYKGGIAQGENNYILSRDLPVECGVNRILLRSTTEAGKIVVRANAEGLSV
                     DSLVLNSLEVPVKDGLSTFFPKDELISYLWRGATPGYASFVMSRLPLSIQSAVAGSNP
                     GDVALSFDDNELSRWDARGPAGEVWIDYTLSRSAVLTEACLKLGNFRNTSYHLRILTE
                     QNEVLWEGQTEKTLGYVNLSLNPASPTRNVRIESLSDGDQARLSIVEAEFYTQIPAQG
                     R"
     gene            3836..>4030
                     /locus_tag="PKY68_06925"
     CDS             3836..>4030
                     /locus_tag="PKY68_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24802.1"
                     /translation="MMSFAGNEISRMSEKDWYIKMILWREKHIKESNFVLILSFVVGL
                     LTSLAALLLKTLIHQIHTWVS"
BASE COUNT          919 a          952 c         1004 g         1155 t
ORIGIN      
        1 cagccgtttt cggccgtggt gtcgtcgtaa aaaacacagt ctttacgctc tgtcagatag
       61 gcaacactca gggaatctcc tttttcgggg aacagctttc tgagaggaag tatagtgccg
      121 ttagcgctgt gtaatctgat gttttcgaaa acagaatcca tcggaaccac caggctgtca
      181 ataaaaacga catcctcgac ggccttcagc atttgttttg ctttttcgga agccttgagc
      241 atacttgcca cttcagccga attggaactt aagccgaaag cgatgtattt atcgaggtgt
      301 ccggcgcttt tgtcgaactg gtattgcttg tggtaatagt ccgccaataa caggctggcc
      361 gctggaattt tgcttttttc tgcgtttatc aggtatggca gggccgctgt tggattgccg
      421 gtatggtaga ggcaggctcc gtaccaatag tttaggttga cgtctttggg tttacgtttg
      481 agttcctgtt cgaaaaccgg tagtgctttt tcatattcac cgttgatata catagccctg
      541 gcctctgtca gggtttgagc cgacagggga acggtaaaaa ggaggaatac tataagcagg
      601 tttctcatgt ccggactgaa tcagttacaa aaatacaaaa ttaaaaacgg attattcggc
      661 ggcagactgt atttttgcgg aaataatgtc tatgactttt ttggagcctg ctcggaaatt
      721 aagtaagaga ctcgattttc gcgtaaagca agctgtcgac aagtacactc ttattcagga
      781 tgatgattca ttcgctttat ctgccctgtg ccgggatctg agtgtaaaat tccgcttcaa
      841 caatactcag tcgggcctga tcgccatcgc ttaggctttc gatgcgaacg ttgcgggtgg
      901 gtgaagcagg atttaggctc agattgacgt agcccagggt cttttctgtt tgtccttccc
      961 agagtacttc gttttgctcg gtaagaattc tcaggtggta gctggtattc ctaaagttac
     1021 ccagtttaag gcaggcttcg gttagtaccg cactgcgcga aagagtgtaa tcgatccaga
     1081 cttctccggc cgggccgcgg gcatcccacc ggctcaattc attgtcgtca aagctcaatg
     1141 ctacatcccc gggattactg ccggcgacag ccgattgtat gctgagcgga agcctcgaca
     1201 tcacaaagga tgcatagccc ggagtagctc cccgccacag gtaagaaatc aattcgtcct
     1261 tgggaaagaa agtactcaga ccgtctttta ccggaacttc caagctgtta agcacgagag
     1321 aatcaacgct cagcccctct gcatttgccc ggactacaat ttttccggct tcggtggtcg
     1381 acctaagcaa aattctgttt actccgcatt ctaccggtaa atcgcgcgaa agaatgtagt
     1441 tgttttctcc ctgggcgatg cctcccttgt attcggccgg gccttcaatg tcgaaatgaa
     1501 tcaggtggtt ggccaaaggg cagcgcagac cgtttttgtc aacaacttct acttggatca
     1561 aaaccatgtc cgatccgtct gcacgaaaac ctttaggatg ttgccagtat tggaggcgca
     1621 gggcggcggc ttcgtctgtg gttttaattt cggcaggttc tcctgccaat tgtcccctgc
     1681 tgtcgtaact gagggctttt agactgccgg cttcgaaggg aacatctctg aacttgaaca
     1741 ggaaatgatg ctgacgttcc ccaaagccca ggttcctgcc gttcagaaac aattcaacgg
     1801 cagaggcatt ggaaaccacc agcacatctt tcacggtgcc cggctcatag ttccagtggc
     1861 ctattatatg gttgctgtat ttattgcctt ctacctcaac ccagccgtcc cacattattt
     1921 tgtgagcgta ataagcctct ttttctatgc gcatggcgtc tacctcacca ctgcggcgat
     1981 agttttcggc tcctcggaaa tgagtgttgg tgtcggaaaa tataatattg acacctccgg
     2041 cacttactcg cctgcctgtc cccggacggt gtacccagaa ctcgtgccag cgtcgtacgg
     2101 cttcttcggc aaaggagtct tggttgcggt tgtaggcaga ggcatcttcg tttctgtaca
     2161 gagggccttc tccgtcgcgg tggtagggat aggaataatt gtcccagtac ttgcgcaggc
     2221 cttcgtcgcg gcaatattca tgggcaaaca tcggtattcc ggcgcttttg ttgatgtaaa
     2281 gcatttcgcc gccgtattcg gcttctttac tgtcgagcat ttcgcgcgat cctatggctc
     2341 tgccaccgtt gggatcgtat atgtcgcgaa tggccttcat ttcggccatg tgttcctcac
     2401 tgatggtttc attgccgcat tcgtaaaaga taatactggg atggttgcgg ttgtagatga
     2461 tggcatcgcg cataagctct ttgcgtaatt cccaggaacg tccctctacg tcactttcgg
     2521 catcgccggc gggcatcgcc tgcagcaggc ctacgcggtc gcaggattct atgtcttgtt
     2581 tccagggagt gacgtgcatc cagcgaacca gattggcatt acctctttcc ataagtccgt
     2641 tgctgaagtc gctcagccag gggggtaccg acatacccac agccggccat tcgttgctgc
     2701 tgcgttgcgc atagcctttc aagtgcaaca ccctttcgtt caggtaaaac atgccgttac
     2761 ggaaggcagt tttgcgtatt cccgtctggg ttcttaccag gtctaaggtt tttttgccgc
     2821 taatcaatga agaggttata ttgtaaaggt agccgtaacc ccagctccag aacctcaggg
     2881 ctttaagatt gcctgaagct ttgacaatgc agctttctcc cggtcgaagg gtgatttcgt
     2941 ctgagttgaa ttcgcggatc atgttgtggt cgttgtctct tactatgact ttcatataga
     3001 aagtttgcgc tttttcctgc tcgttcttca cttccgattc cacattaagt acggctgttt
     3061 tgtctcttac attgatgtcc gtggcatata cataagtgcc cacagtgccc agatgactgt
     3121 acaaggggag cgtctggtag agcttgccgc aaatatgcaa aacaacgttt ttggtcaagc
     3181 ctccgtaatt ggcgttgaaa ttgttgttgt tccactgaaa gccggtacct gtggcctttt
     3241 ctttgtattt ccaatcgttg tctatcctga gggcgattac gttttctttg tttccgaaaa
     3301 ccagatgatc tgtcaggtcc agaccgaaag ccatcactcc gttttcgtgc aggccaatct
     3361 cccggccgtt gatccatatt tcggcagcct gacgcaggcc ttcaaattca atgaatactt
     3421 tttgattttt gtaagtcgag ggtattttaa aatgtttgcg ataccatacg atggtatcgg
     3481 tcaaatcgtg gatggatttg gcaaaggctt catcctcgtt gaaggcagcg ggcaggtgaa
     3541 cggttttcca ttcagtgtcg tcaaattccg gattgtgagc gccgggttgg tcgccgatgt
     3601 ggagcagcca ggagggattg aagttaaact taatacgagc gggatttgcc gccaaagttg
     3661 tgccggcaat taaaactgcg gcgaaaaaaa gagcggctgt ttttctgcgt gtgttcatga
     3721 actttactat tggaatatac ctcgaaacag acggatgata gcaggttttt taacgaaggg
     3781 caaacttaca taaaaagcgg ttaaaactga aatcatcttg cagatttgaa ttaaaatgat
     3841 gtcctttgcc ggcaacgaaa tttctcgaat gagcgaaaaa gattggtata tcaagatgat
     3901 tctttggaga gagaagcaca tcaaagaatc caacttcgtt ctgatactga gctttgtggt
     3961 aggcttgctc acatcgttgg cagccctgtt gctgaaaacc cttatccatc agatacatac
     4021 ctgggtttct
//
LOCUS       DAOSGL010000213         4028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_20296_length_4028_cov_3.739743, whole genome shotgun sequence.
ACCESSION   DAOSGL010000213 DAOSGL010000000
VERSION     DAOSGL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20296_length_4028_cov_3.739743"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="PKY68_06930"
     CDS             <1..1593
                     /locus_tag="PKY68_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOH24803.1"
                     /translation="MARKAYFTSKWQIETGGILLLLGAIIIVVSLRYYFSATSKIEEP
                     SEVKLSQNMARLLSSKWILGLGALVFVLALGSSFLSVNQLNNYDPLSVAVQDSTVTTQ
                     EAIEVIDLSTSSNTPTADTAATSVPANAPADSVAAPVAVVAQTPSLSDIKKQSNSFRG
                     PLGNGVWTSKNIPSSWDIAAGTNVKWKVKVPKKGYNSPVIWGDKLFVSGADATERWLY
                     CYDRNTGNLHWQHQANNITGSPATPPKTTDDTGLAAPSVTTDGNIVVAIFGTGDIVAC
                     DYSGNRVWAKNLGVPANHYGHSSSLIYWKGKVIVQFDTNKERKLMALDINTGNSVWET
                     PRTSHISWASPILAEIGGKMQVVTSSEPTVAGYDAETGKELWKNDCMMGEVGPSPAFG
                     SGLVFAANEYATLAAIDPSNGSKVWENNEYLPEVASPVVSEGLLFIATSYGVLACYDA
                     KSGTKHWEEDFGEGFYSSPVVADGKLFACDTKGVVHIRKVAAEASKIADIPMDQKIMS
                     TPSFANGCIFIRSVENLYCIGN"
     gene            1638..2600
                     /locus_tag="PKY68_06935"
     CDS             1638..2600
                     /locus_tag="PKY68_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24804.1"
                     /translation="MQNKMIKYFLLVLAVLIVAIIATNYIGNKPKNAKQNPYEYNVEE
                     FKTVPDSLISHREVRQVKITVKQHGSLTSFDNTIWFSADNSVKNLSLEGKILADFSVS
                     EPVRAIAVNSDLVVVAYEKKFAVYSHVGEKAFESDAITDSTFITSVAFFNGKIVIADA
                     GKRKVYIYNKNQKEREIEGISGAKNLHGFVIPSPYFDVAVSASNELWAANTGMHSLQH
                     YDENGNLLDSWEKISNNIDSFCGCCNPAHFALMTDGRFVTSEKGLTRIKIYSQKGELE
                     SVVASPDVFVEEGFAPEVAVIGDTVVALDFDKKMIRIFGKSESK"
     gene            2798..3850
                     /gene="rfbB"
                     /locus_tag="PKY68_06940"
     CDS             2798..3850
                     /gene="rfbB"
                     /locus_tag="PKY68_06940"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOH24805.1"
                     /translation="MKKIIITGGAGFIGSHVVRRFVRKYTDCQIFNLDALTYAGNLEN
                     LSDIKDCSNYTFVKGDITDGDFITKLFEEHQFDGVIHLAAESHVDRSITNPMSFILTN
                     IVGTVNLLNAANHIWKGNYEGKRFYHISTDEVYGSLGEEGFFTEETHYDPQSPYSASK
                     ASSDHLVRAYHNTYGLPVVLSNCSNNYGPNQFPEKLIPLIINNIENNKPLPVYGQGLN
                     VRDWLYVEDHASAIDLIFNEGEIGETYNIGGNNEWRNIDIVKKICEIMDRKLGRENGT
                     SEKLITYVKDRAGHDLRYAIDATKIKKCLGWEPSLQFEEGIEKTVDWYLANKDWMERI
                     TSGDYQKYYEEMYSQR"
BASE COUNT         1216 a          855 c          912 g         1045 t
ORIGIN      
        1 atggcgcgca aagcctattt cacttcgaag tggcagatcg aaaccggggg aatcctgctc
       61 ttgctgggag ccattatcat tgtggtgtcg ctgcgttact atttcagcgc gaccagcaaa
      121 attgaagaac cttcggaagt aaagctttct caaaatatgg cccgattgct ttcttcaaaa
      181 tggatactgg ggctgggagc attggtgttt gtgctggcgt taggatcgtc gtttttatcg
      241 gtcaatcaat tgaacaacta cgatcctcta tcggtagcgg ttcaggacag tactgttaca
      301 acgcaggaag ctattgaggt gatcgatctc tctacctcat ccaatacgcc aactgccgat
      361 acagcagcaa catcggtacc agcaaatgct cctgccgatt ctgtggctgc acctgttgct
      421 gtagtcgccc aaacgccttc tttgtctgac atcaaaaagc aatcgaatag cttccgaggc
      481 ccattgggca acggggtttg gacgagcaaa aatatcccgt cgtcgtggga catcgcggcc
      541 ggtaccaatg tgaagtggaa ggtgaaagtt ccgaagaaag ggtacaattc tccggtaata
      601 tggggcgata aactttttgt atcgggtgcc gatgccaccg aacgctggct ttactgctac
      661 gaccgaaaca ccggaaatct gcactggcaa catcaggcca acaacattac cggatcaccg
      721 gccacgccgc ccaaaactac cgatgacacc ggactggcag ctccatcagt caccaccgac
      781 ggaaacattg tggttgccat attcggtacg ggagacattg ttgcatgcga ctattcaggc
      841 aaccgtgtgt gggctaaaaa ccttggcgtt ccggccaacc actacggcca ctcgtcatcg
      901 cttatctact ggaaaggaaa ggtaattgtg cagttcgaca ccaacaagga gcgcaagctg
      961 atggcactcg atatcaacac cggaaattcg gtttgggaaa cgccgcgtac ctcgcacatt
     1021 tcgtgggcat cgcctatcct ggccgaaatt ggaggaaaga tgcaggtcgt tacctcgtcg
     1081 gagcctacag tggcaggcta tgatgccgaa accggaaaag agctttggaa aaacgattgt
     1141 atgatgggtg aagttgggcc ttcgcctgct tttggcagcg gattggtatt tgctgcaaat
     1201 gaatatgcca ccctcgccgc aattgaccct tcgaacggat ctaaagtgtg ggagaacaat
     1261 gaatatcttc ccgaggttgc cagtccggtt gtctctgaag gattgctctt tattgcaaca
     1321 agttatggtg ttctggcatg ttacgacgct aaaagcggaa caaaacattg ggaagaagat
     1381 tttggcgaag ggttctactc aagtcctgta gttgccgacg gcaaactctt cgcatgcgat
     1441 acaaaaggag ttgttcatat tagaaaagta gccgccgaag cttcaaaaat tgccgacata
     1501 ccaatggatc aaaaaattat gtcgactcct tcgtttgcta atggatgcat ttttattcgc
     1561 agtgtagaaa acttgtattg cattggcaat tagtaacatt ttcaaaaaat atttgtttat
     1621 tcaattaaaa ctgcgctatg caaaacaaaa tgataaaata ttttttgctg gttctggccg
     1681 tactcattgt tgcgattatt gccacgaatt acattgggaa caagcccaaa aatgctaagc
     1741 agaatccata tgaatacaat gttgaagagt ttaaaacggt tcccgattca ttgataagtc
     1801 atcgtgaggt aaggcaggtt aaaattacgg tgaaacaaca cggaagcctt acatctttcg
     1861 ataatactat ttggttttca gcagataaca gcgtgaaaaa tctttctctg gaaggcaaaa
     1921 tccttgctga tttttcagta agcgaacccg ttagggctat agctgtaaac agcgacctcg
     1981 ttgttgtagc ctacgaaaaa aagtttgccg tctattctca cgttggcgaa aaagcatttg
     2041 aatcagatgc gattaccgat agcactttca tcacctcggt ggcctttttc aatggaaaaa
     2101 ttgtgattgc tgatgccgga aagcgcaagg tctacatcta caacaaaaat cagaaagaaa
     2161 gagagataga aggtatttcc ggtgcaaaaa acctgcatgg tttcgttatc ccaagtccct
     2221 attttgatgt tgccgtcagt gcatcgaatg aactttgggc agccaatact ggcatgcatt
     2281 cgctccaaca ctacgatgaa aatgggaatc tgctcgattc ctgggagaaa atatcgaaca
     2341 acattgatag tttctgcgga tgttgtaatc cggcacattt tgcgttaatg accgatggac
     2401 ggtttgtcac cagtgaaaaa gggcttacga gaattaaaat ctacagtcaa aaaggagaac
     2461 tcgaatcggt tgttgcaagt cctgatgttt ttgtagaaga aggatttgcc cctgaagtcg
     2521 ccgtgattgg cgatactgta gttgccctcg atttcgataa aaaaatgatc aggatctttg
     2581 ggaaaagtga gtccaaatag ctgaaaagtc aatattcgaa ttcatttttg catcaagtcc
     2641 cttcagtatc cataataaaa tgctccgcgg gtagtttttt ccatagtaaa tgtttattgc
     2701 aaaatagatt ggtataccta tttttgtact aaaagataca acattcaaat tgcatccgtt
     2761 taagcttatc agcaagtaaa atttatacgt aattaaaatg aaaaaaatta tcataaccgg
     2821 aggcgccgga ttcattggtt ctcatgtggt tcgcagattt gtaagaaaat ataccgattg
     2881 ccagatattt aacctcgatg cattgaccta cgctggcaat ctcgaaaact tatccgacat
     2941 aaaagattgc agcaattata cttttgtaaa aggcgacatc accgacggag acttcataac
     3001 caagcttttc gaagaacacc aattcgacgg agtgatccat ttggccgccg aaagccatgt
     3061 cgaccggtcg attacaaacc ctatgtcatt cattttgact aatatcgtcg ggacagttaa
     3121 cttattgaat gccgcaaacc acatctggaa aggaaattac gaaggcaaac gtttttacca
     3181 tatctcgacc gacgaagtat atggttcgct gggagaagaa ggatttttca ccgaagaaac
     3241 ccattatgat ccgcaaagcc cttattctgc atcgaaagcc agttcagacc acttggtaag
     3301 ggcataccac aacacctatg gtttgccggt agtactttcg aactgttcaa acaattacgg
     3361 accaaatcaa ttccctgaaa aactcattcc gctaataatt aacaacatag aaaacaacaa
     3421 accccttccg gtatacggtc agggcttaaa cgtacgcgat tggctttacg ttgaagacca
     3481 cgcctcagcc atcgatctga ttttcaatga aggggaaatc ggcgaaacat acaatatcgg
     3541 gggcaacaac gagtggcgta acatcgacat agtgaaaaaa atatgtgaga taatggatcg
     3601 caaactgggc cgcgaaaacg ggacatcaga aaaactgata acgtatgtga aagaccgtgc
     3661 aggccacgat ttgcggtatg ctattgatgc aacaaaaata aaaaaatgct taggctggga
     3721 gccctcactt caatttgaag aaggaattga aaaaacagtt gattggtacc ttgccaataa
     3781 agattggatg gaaagaataa cctcgggcga ctatcagaag tattacgaag aaatgtattc
     3841 acaacggtaa cattggaaaa cctgctgatt ataactcctg taaaagattc gattgttgtt
     3901 gcaagcgagg ccattacgcg tgtttgtcaa tcgaaaaaaa attatcggta ccgtgtttac
     3961 aacgatagaa gtttgcccga gagccgtacc gtgctcgaac atggttctat tttaggttac
     4021 gaactgat
//
LOCUS       DAOSGL010000214         3951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_20905_length_3951_cov_4.147074, whole genome shotgun sequence.
ACCESSION   DAOSGL010000214 DAOSGL010000000
VERSION     DAOSGL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20905_length_3951_cov_4.147074"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKY68_06945"
     CDS             <1..269
                     /locus_tag="PKY68_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOH24806.1"
                     /translation="AAIVPAIGKSKAPGSPIAGRANVLVFPSLEAGNIAYKLVQRLAS
                     AEAIGPILQGMAAPINDLSRGCSVDDIYKMIVITCNQSLGIKAN"
     gene            277..1488
                     /locus_tag="PKY68_06950"
     CDS             277..1488
                     /locus_tag="PKY68_06950"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOH24807.1"
                     /translation="MKILVLNCGSSSVKYKLFDMDSSKVLGQGSVEKIGLSGSYLKLQ
                     TSSGERVLIEEDFPEYTKSIQFIFKMLTESKYACIKSLKEIDAVGHRVVHGGEKFSKS
                     VRINDEVLRMVNECIDLAPLHNPPNLKGIEAVSKLMPDLPQVAVFDTAFHQTMPAKAY
                     MYGLPYEYYKKYGIRRYGFHGTSHRYVSRRACDILGVDYASQRIISAHIGNGGSVTAI
                     RDGKSVENSMGLTPLEGLLMGTRTGDIDGGALTYLMEKENIGPRALNNILNKKSGILG
                     VSGVSSDMRELTAAAKSGNKRAQLAFDMYNYMIKKYVGAYAAVLGGVDILIFTGGVGE
                     NQDETRQAICENMEYMGLLLDVEKNKLIRGKEAVISAPESKVKAMVVPTNEELMIAMD
                     TVEILSQGRYQ"
     gene            complement(1494..2366)
                     /locus_tag="PKY68_06955"
     CDS             complement(1494..2366)
                     /locus_tag="PKY68_06955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH24808.1"
                     /translation="MHKEEIINKTMDLEDLNSERALQIVDELNDLIKALRSKNLKISS
                     WYGSFNLSGDPLSNERINRGYGYQSLEGAADDKNFPWFLYWEIVWVVLNNDFKQGQEV
                     LDLGGSSSLFSYYLASKGIKVTAIDLQKELVDNANHVAKQMNWNLENRVMDMRALDFN
                     STFHHITSICVFEHIPMYDRVIINEKIRNLLMKDGKFSITFDYRNPSAFAQINSPQDV
                     YEQFVKPSGLKIRGNKEFADNGKNYLLHPFYSKKKLWNYKRISIQKGHFKPWDFFKTK
                     STNDYTFGALFLER"
     gene            2613..3146
                     /locus_tag="PKY68_06960"
     CDS             2613..3146
                     /locus_tag="PKY68_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH24809.1"
                     /translation="MEFSTLVKQRQSDRKYEPKAVEKEKILQCLESARLAPSANNSQP
                     WKFVVVDQPELKEQIAECAVSLGLNKFTHQAPVIVALVMEKVNLLSGMGSVIKDKEFA
                     LLDLGIVVNQFCLQAAELGLGSCIIGWFDEPKVKKLLNIPAKKRVPLLIALGYPASKT
                     REKTRKKLEEISSWNSY"
     gene            complement(3155..>3951)
                     /locus_tag="PKY68_06965"
     CDS             complement(3155..>3951)
                     /locus_tag="PKY68_06965"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOH24810.1"
                     /translation="ACNTARNASIGMSGPFDFRNNVIFNWRHRTIDGGDETSLINLIN
                     NYYRPGPATNENMRAVFARIEQRDMYSPGSAWAEGGWFPRAEKRPGKWYVAGNVMHDN
                     EEVTNNNWAGMRGPENLARVNTPFEGWPVAPHQTAQEAFESVLSHAGATLPKRDAVDK
                     RVTEMVRSGKTTTETGIIKDISEVGGYPELTYDPKEVPVDTDGDGMPDEWEKKHNLDP
                     NDPSDGAKDADKDGYTNVEEYLNGTDPNEYINYRNLGNNVDMISFK"
BASE COUNT         1020 a          841 c          972 g         1118 t
ORIGIN      
        1 atgccgctat agtgcccgcc atcggcaaaa gtaaggcgcc gggcagtcct attgccggaa
       61 gggccaatgt tttggttttc cccagtttgg aggccggaaa tattgcttat aaactggtac
      121 agcgtctggc ttcggccgag gctataggac ctattctgca gggcatggct gcgcccatta
      181 acgatctttc gagaggatgc tccgtggatg atatttataa gatgattgtt attacctgca
      241 atcaatcgtt ggggattaag gctaattgat ataattatga aaatactcgt tttgaattgc
      301 ggaagttctt ccgtgaagta taagttgttt gatatggaca gctccaaggt cttggggcag
      361 ggttccgtag aaaagatcgg tctgagcggt tcttatttga aattgcaaac cagcagcggc
      421 gaaagggtgc tgatcgaaga agattttccc gagtatacca aaagcataca atttattttc
      481 aagatgctga ccgaaagtaa atacgcctgt ataaaatctc tgaaagaaat agatgcggta
      541 ggacaccggg tggttcacgg aggcgaaaaa ttcagtaaga gcgtacgtat caacgacgag
      601 gtgttgcgta tggtaaacga atgcattgat ctggctcctt tgcacaatcc gcctaatctc
      661 aaaggaatag aggctgtaag caagctgatg cccgatctgc ctcaggtggc tgtttttgat
      721 accgcttttc atcagaccat gcctgccaaa gcttatatgt acggccttcc ctacgaatat
      781 tacaagaaat acggcattcg tcgttacggc ttccacggca ccagtcatcg ctatgtgtcg
      841 cgcagggcct gtgatattct tggggttgat tatgcttccc agcgcattat ttcggcgcac
      901 ataggtaatg gcggctcagt gacggctata cgcgacggca agtcggtgga gaattccatg
      961 ggactgactc cgctggaagg tttgcttatg ggtacccgca ccggcgatat cgacggtgga
     1021 gcattgacct atttgatgga aaaagagaac atcggccccc gtgccttgaa taatattctc
     1081 aacaagaaaa gcggtattct gggggtttcg ggggtttctt cggatatgag agaactcact
     1141 gcagccgcaa aatcgggaaa caagagggct cagctggcat ttgacatgta taattacatg
     1201 atcaagaaat atgtgggcgc atacgcggca gtattaggtg gtgtggatat acttattttc
     1261 acaggggggg tcggcgagaa tcaggatgaa acccgtcagg ctatttgtga aaatatggaa
     1321 tacatgggtt tattgcttga cgtcgaaaaa aacaagctga ttcgtggtaa agaagccgtt
     1381 atttctgctc cggaatccaa agtcaaagcg atggtggttc ctaccaatga ggaacttatg
     1441 attgctatgg atacagtgga aatattgtcg caaggccgct atcagtgacc ggcttaacgt
     1501 tcgaggaaca gggccccaaa ggtatagtcg ttggtgcttt ttgtcttaaa aaaatcccag
     1561 ggcttaaaat gccccttttg aatggaaatt cttttgtaat tccacaattt ctttttggaa
     1621 taaaaggggt gcaataaata attcttacca ttatcggcaa attctttatt ccctcttatt
     1681 ttcaagcccg agggttttac aaattgttca taaacatcct ggggagaatt gatttgggca
     1741 aatgctgagg gatttctgta atcgaaggta atactgaatt tcccgtcttt catcaacaag
     1801 tttctgattt tttcgttgat gattactctg tcgtacatgg gtatatgttc aaatacgcaa
     1861 atggaagtga tgtgatggaa tgttgaattg aaatccaatg ctctcatgtc catgacgcgg
     1921 ttctctagat tccaattcat ttgctttgcc acatggtttg cattatcaac caattctttt
     1981 tgaaggtcta tagcggtcac cttgatccct ttggaagcca ggtaataaga aaataaggac
     2041 gagcttccac ccaaatccaa cacctcctgg ccttgcttga agtcattatt taaaaccacc
     2101 cagacgatct cccaatacaa aaaccaggga aaatttttat catcggctgc gccttcgagg
     2161 ctttggtagc cgtatccccg attaattctt tcgttagaaa gggggtcacc gctcaggttg
     2221 aaacttccgt accaggaact gatttttaaa ttttttgacc tcaatgcctt gattaagtca
     2281 ttaagttcat cgactatttg taaagccctt tccgaattaa ggtcttccaa atccatcgtt
     2341 ttgttgatta tctcttcttt gtgcataatt tgagcactgt ctttgttttg cttgctatac
     2401 gagctaaccc agggttacat tggcaatttc attgtcgggc actgtctttt ttgtacctac
     2461 tatcccgggc aaatataact gtttttgact gcttggtcga gaaaaacagg cactcaaaga
     2521 catctgattt ttacactttg cctatgatcg tcaagagacg gtaatttttt gtatgtttgc
     2581 tcaaataaaa attcaatcaa tacatagtcg atatggaatt ctcaacatta gtaaagcagc
     2641 gtcaaagcga cagaaaatac gaaccaaagg cggtagaaaa agaaaaaatc ctgcaatgcc
     2701 tcgagtcggc ccgcctagca ccttcggcga ataattcaca gccctggaaa tttgtggtgg
     2761 tcgatcaacc cgaattgaag gaacaaattg cagaatgtgc ggtgagcctg gggctaaata
     2821 aatttacgca ccaggcaccg gttatcgtcg ccttggtaat ggaaaaggtc aacttactct
     2881 ccggtatggg ctctgttatt aaggacaaag aattcgccct gctcgatttg ggcattgtcg
     2941 taaaccagtt ttgcctgcag gctgccgaat taggcttggg aagctgtatt ataggctggt
     3001 tcgacgaacc caaagtcaag aaactattga atatccccgc aaagaaacgg gttcccctgc
     3061 tcatcgctct gggttatccg gcttcgaaaa ccagagaaaa aacccgtaaa aaactggaag
     3121 aaataagtag ttggaattcg tattgattcc ccttttactt gaagctgatc atatcgacgt
     3181 tgttccctaa attccgatag ttgatgtact cgttgggatc ggtgccgttg aggtattcct
     3241 ctacattggt gtatccgtct ttgtcggcat ctttagcacc atccgaagga tcgttgggat
     3301 ccagattgtg tttcttttcc cattcgtcgg gcatgccgtc gccgtcggta tcgacgggaa
     3361 cttctttggg atcgtaagtg agttcgggat agccgcccac ttcggagatg tccttgatga
     3421 taccggtttc ggttgtggtt tttccactgc gtaccatttc agtgacgcgt ttatcgacag
     3481 cgtcgcgttt gggcagggtg gccccggcgt gggaaaggac cgactcaaaa gcttcctgag
     3541 cggtttgatg cggagcaaca ggccaacctt cgaaaggagt atttacgcgg gccagatttt
     3601 cggggccgcg cataccggcc cagttgttgt tggtgacctc ctcgttgtcg tgcataacgt
     3661 taccggcaac ataccatttt ccgggacgtt tttcggccct gggaaaccag ccgccttcgg
     3721 cccaggcact gccgggtgag tacatatctc gttgttcgat gcgggcaaag acggcacgca
     3781 tattttcgtt ggtggcggga ccgggtctat agtaattgtt gatcagattg atcagagagg
     3841 tttcatcgcc cccgtcgatt gtgcggtgtc gccagttgaa aatgacgttg ttgcgaaaat
     3901 caaatggtcc cgacatacca atggaggcgt tgcgtgcagt gttgcaggca a
//
LOCUS       DAOSGL010000215         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_20938_length_3945_cov_4.591003, whole genome shotgun sequence.
ACCESSION   DAOSGL010000215 DAOSGL010000000
VERSION     DAOSGL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20938_length_3945_cov_4.591003"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKY68_06970"
     CDS             complement(<1..236)
                     /locus_tag="PKY68_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOH24811.1"
                     /translation="MRLVAPSLLSADFARLEADLTMINESQADWLHLDVMDGVFVPNI
                     SFGFPVLEAVARVCTKPLDVHLMIVEPERHIESFA"
     gene            complement(285..449)
                     /locus_tag="PKY68_06975"
     CDS             complement(285..449)
                     /locus_tag="PKY68_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24812.1"
                     /translation="MEKISTPQTQSSVKNKSVFLIEPKASTLDFLRIFARVYPNNELA
                     VPGKSVLSLN"
     gene            complement(570..1163)
                     /locus_tag="PKY68_06980"
     CDS             complement(570..1163)
                     /locus_tag="PKY68_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH24813.1"
                     /translation="METHRQMTDEALVSRFANGDNQAFDILLNRHKNRVYTYILLIVR
                     NRDLAEDVFQETFMKAIVTIKQGRYVDSGKFYAWVTRIAHNLIIDIFRKERNENTISN
                     DEFEDVDLFNNPKFSDENVEDRLVKNQVMKDVSSLVRMLPDSQKEVVVLRYYKNLSFK
                     DIADQTGVSINTALGRMRYAVLNMRRMAEGKDLVLSF"
     gene            complement(1287..2258)
                     /gene="fmt"
                     /locus_tag="PKY68_06985"
     CDS             complement(1287..2258)
                     /gene="fmt"
                     /locus_tag="PKY68_06985"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOH24814.1"
                     /translation="MRKQELKIVYMGTPEFAVAPLQALIDNHYNVVAVVTMPDKAMGR
                     GLKVQASPVKQLARSYSLPVLQPDNLKDPVFLEKLRSYDADLQIVVAFRMLPEAVWAM
                     PPMGTFNLHASLLPQYRGAAPIQWAVINGEKQTGLSTFLLDRQMDTGRILLQEKIAIG
                     PDETGGSVYSRLMELSGPMVLRTVDLLLSGQSRPVDQNTLIKEAEPLKTAPKLHKENT
                     RIDWSRPGAELYNFIRGLAPYPAAWTAWQKSGESIQTVKIHQAAFVAQEHHMECGKLL
                     SDQKTYIKTPVPDGFLHWEFFQPAGKKILSVAEFLRGRPGIEDFKLL"
     gene            complement(2261..>3945)
                     /locus_tag="PKY68_06990"
     CDS             complement(2261..>3945)
                     /locus_tag="PKY68_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOH24815.1"
                     /translation="LLTSLAALLLKTLIHQIHTWVSSGSAIDHANYYYLLYPALGIFL
                     TALFVRYVVRDDIGHGVTKILYAISRRQGRIKRHNTWTSMVSSSITIGLGGSVGAEAP
                     IVLTGAAIGSNLGSFFKMDHKTLMILIGCGAAGTIAGIFKAPIAGLLFAIEVLMIDFT
                     VASIMPMLVSSVTAATLAYFMSGTGVAISFDGADVFQLVNVHWVIVLGIFCGLVSLYV
                     IRGMNTIEGWYKKIKNPWLKIILGALVLGLLIFLFPPLYGEGYESIEALINGNLAELT
                     HNSLFYGARQHFGLLALYLLLIIIFKVFASASTNGAGGVGGVFAPSLFVGSFTGFLVA
                     TAINYFGLGTVSPGNFALMGMAGLMSGVMHAPLTGMFLIAELTGGYDLFLPLMIVASV
                     SFMTIFLFEKHSIYSMRLAQKGQLLTHHKDKAVLTLMRIENLIEKDFDVLSPDMTLGD
                     LVKVISKAKRNMFPVVDKRGVLIGVVLLDDIRNIMFRQELYGRFKVTNLMISPPAVLN
                     ISDSMEEVMKIFDDTNAWNLPVKDDEGRYVGFVSKSKIFNSYRRVLVHFSDE"
BASE COUNT         1101 a          934 c          906 g         1004 t
ORIGIN      
        1 gcgaagcttt cgatatggcg ttccggttcc acgatcatca ggtgcacatc caaaggcttg
       61 gtacatacgc gggcaacggc ttccagcaca gggaaaccga aggaaatgtt gggcacaaac
      121 acgccgtcca ttacatcgag atgcagccag tcggcctgac tttcgttaat catcgtaaga
      181 tctgcctcca gacgggcaaa atcggcagac agcaaagaag gagcaaccaa acgcatggtg
      241 gaaaattagt tacgaaatac aagccctgat taaaagagct cggtttaatt taaacttaac
      301 acagatttac ccggtactgc taattcgttg ttggggtaaa ctctggcaaa tatacgtaaa
      361 aaatccaggg tggatgcttt gggttcgatc agaaagactg atttattttt cactgaagat
      421 tgagtttgcg gtgtagaaat tttttccata ggcgtataat taaaaaagcg tttattctat
      481 aacgaggatt tttgttccat cgtataaaac aaacgccttt ttttacacca aaaaacaact
      541 tttgagataa ctatttgtgt atctatggtt taaaaagaca acactaagtc ttttccttcg
      601 gccatccggc gcatattcaa cactgcatag cgcatgcgac caagggctgt gttgatactg
      661 acacctgtct gatcggcaat atccttaaag cttaagtttt tgtaatagcg caataccacc
      721 acctcttttt ggctgtccgg cagcatgcga accagactgc tgacgtcttt catcacctga
      781 ttttttacca acctgtcttc gacgttctcg tccgagaact taggattgtt gaataagtcc
      841 acatcttcaa actcgtcgtt cgaaatagta ttctcgttgc gttctttgcg aaaaatatcg
      901 atgatcagat tgtgtgctat acgcgtaacc caggcataaa acttgccgct gtccacataa
      961 cggccttgtt taatggttac aatggctttc ataaaagttt cttgaaatac atcttcggcc
     1021 aaatccctat tacggacaat caacagtata taagtataca ccctgttttt gtggcgattc
     1081 aataaaatgt caaaagcttg gttgtctcca ttcgcaaacc tgctgaccaa tgcttcatcg
     1141 gtcatctgtc ggtgtgtttc catctctctt ttttaaaacg ttaaaaagag tagtattgga
     1201 tcaataggca aatggtttta atggttagtg ctgcaaatga aagaggattt ttttaaatct
     1261 gcaagtattt aagcattttt ttgtcgtcag agcagtttaa aatcctcgat accgggcctg
     1321 cctctcagaa attcggctac cgaaaggatt ttttttcctg caggctgaaa aaattcccag
     1381 tgcaaaaaac catcgggtac cggggtcttt atataggttt tttgatcgct cagcagtttg
     1441 ccgcattcca tatggtgttc ctgagccaca aaggcagcct gatggatttt taccgtctga
     1501 atgctctcgc ctgatttttg ccaggctgtc caggcggctg gataaggagc caggccgcga
     1561 ataaaattat aaagctcggc tccgggccgc gaccagtcga tacgtgtatt ttctttgtgt
     1621 aacttgggag ccgtctttaa gggttctgct tctttgatca atgtattctg gtccacaggc
     1681 cggctttggc cgctcaagag caaatctacg gtacggagca ccatggggcc ggaaagttcc
     1741 atcagccggg aataaacgga tccgccggtt tcgtcgggac caatggcaat tttttcctgt
     1801 aaaagaatgc ggccggtgtc catttggcgg tcgagcaaaa aagtgcttag accggtttgc
     1861 ttttcgccgt tgataacagc ccattgtatg ggagcggctc ccctgtattg gggcaagagg
     1921 gaagcatgca ggttaaaagt tcccatggga ggcatggccc aaacggcttc gggcaacatc
     1981 cgaaaagcta caactatctg caaatccgca tcgtaagagc gcagtttttc caaaaaaacg
     2041 gggtctttca ggttatcggg ttgcagcacg ggcaaggagt aagaccgggc cagttgtttt
     2101 accggcgaag cctgcacttt aaggccccgc cccatggctt tatcgggcat agtgactacg
     2161 gctactacat tataatggtt gtcaatcaaa gcttgcaggg gggctaccgc aaactccggc
     2221 gtacccatat acactatctt caactcttgt tttcgcataa ttactcgtcg gaaaaatgga
     2281 ccaacaccct gcgataggag ttgaaaatct tggatttgga cacaaagcct acatagcggc
     2341 cctcgtcgtc ctttaccggc agattccagg cattggtatc gtcgaaaatc ttcattacct
     2401 cttccataga gtcgctgatg tttagtacgg ccggcggcga aatcatcaga ttggtcactt
     2461 tgaaacgccc gtataattct tgacgaaaca taatgttccg gatatcgtcc agcaaaacaa
     2521 caccgatcaa taccccccgt ttgtcgacaa cgggaaacat attgcgtttg gctttggata
     2581 tcacctttac caaatctccc agggtcatat cgggactgag cacatcaaag tctttttcga
     2641 tcaggttttc gatcctcatc aatgtaagta cggccttgtc tttgtgatgg gtcagcagtt
     2701 ggcctttttg ggccagccgc atggagtaaa tactgtgttt ctcaaacagg aagatggtca
     2761 taaacgaaac cgaagccact atcatcaaag gcaggaacaa atcataacct ccggtaagtt
     2821 cggcaatcag gaacatcccc gtaagcggag catgcataac gcccgacatc agtccggcca
     2881 tgcccatcag ggcaaaatta cccggagaca cggtgcccag gccgaaataa ttaattgctg
     2941 tagccaccag aaagccggtg aaacttccca caaacaggct gggagcaaaa actccgccta
     3001 ccccgccggc accgttggtc gaagctgaag caaaaacctt gaatataatg atgagcagca
     3061 aataaagagc cagcaagccg aaatgttgcc gggcaccgta aaagaggctg ttgtgagtta
     3121 attcggccag gttgccgttg ataagtgcct ctatactctc gtaaccttct ccgtacaaag
     3181 gagggaacag gaagatgagt aaacccagaa ccaaagctcc taatatgatc tttaaccagg
     3241 gatttttgat ttttttatac caaccttcga tggtgttcat cccccggatc acatacaagg
     3301 agaccagtcc gcaaaaaatg cccaagacaa taacccagtg tacattgacc agttgaaaaa
     3361 catcggcacc gtcaaaagaa atggccacac cggtacccga cataaagtag gccagggtag
     3421 ccgcggtcac tgaagatacc agcatcggca taatagaggc caccgtgaaa tcgatcatca
     3481 acacctcgat ggcaaacagg agtccggcaa tgggcgcctt gaaaatgccg gcaatggttc
     3541 cggcggctcc gcaaccgatc aaaatcataa gggtcttgtg gtccattttg aagaaacttc
     3601 ccaagttgga accaatggca gcgccggtta acactatagg tgcttctgct cctaccgaac
     3661 cacccaaacc gatggtaatg gagctggaaa ccatcgaagt ccaggtatta tgtcgtttga
     3721 tcctgccctg gcgacgtgaa atggcgtaca gaatcttggt tactccgtgt ccgatgtcgt
     3781 ccctgaccac atagcgaaca aacagagctg tcaaaaaaat acccagagcc ggatacagta
     3841 agtaataata gttagcgtgg tcaatggccg atcccgaaga aacccaggta tgtatctgat
     3901 ggataagggt tttcagcaac agggctgcca aggatgtgag caagc
//
LOCUS       DAOSGL010000216         3935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_21026_length_3935_cov_3.942268, whole genome shotgun sequence.
ACCESSION   DAOSGL010000216 DAOSGL010000000
VERSION     DAOSGL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21026_length_3935_cov_3.942268"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..>3935)
                     /locus_tag="PKY68_06995"
     CDS             complement(180..>3935)
                     /locus_tag="PKY68_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOH24816.1"
                     /translation="LLIDKVDLPFYAELRNQRTDNWKDIGGHFIINYLDGIRPSLVYM
                     YKNRAENGWFYGAHAAFSYRNNKLIKDWAYRFWPNQAEFHQVRAADVDGDGRDNFVEG
                     GYVLKHDGTILNRHQDAVHGDRHCLADIDPDRPGLEHFIIQQNNPKTLGMGLFDAATG
                     AIIKCNYQSAVGDVGRGICGAFDVTCRGMQYWSTMNGNAMYDCKGDLIEGAAGTFPSE
                     ALWWGPGLARWEISGIGSGGPNLAFHVYNPSNKSFYRDQPNLYNEYIPYYFKSINAGR
                     AAFWGDMLGDWREELICVRSDWTGFVVLSTWEQTNHRIYHLMQNPAYRLQTTAKGYYQ
                     TPDVDFYMAADMPKPPIAPVQQADLYYSGSGWMDREEQAADYADGKSVMFDLRGGNDT
                     FNIEGNFSPSVLYLMNPKDADYTLEGSGKITGSTRLIKSMQGDIVLRGQHDYTGSTRI
                     SEGRLWLDGSLDSPVQIDARGVLGGRGVLKGGVVLEQGLNREGGRIEPGFAGRPDTLN
                     ILGNLSLRGRNNLAFDISEEACDLLQIQGDLLLSGSENSLIIQAAGELPKEQEMTLIT
                     FSGSTNAKKGDFRLFGLEGLPYDLNIAEHSITLKIKSSREPASVLWMGNENNTWDFAT
                     PNFNWQNGASIFVPGDSVRFTDAAVYKQIVINETMPVGQLMFENEEAFSLSGRGVVSG
                     EGGLSKSGGGLLSIQNTDNSFTGPVIIENGILEVASLKDGGLPSSIGASSNSSQNWIM
                     RNATLQTRSQMSTNRHMQVVDTLTINNPASNNSVLISGNMSGANTTLIVKGKGSLTLQ
                     GSNTFGRVILKDGLLLLGSSHANRYSMGSAKIRLEGGIFRMHNINTTSDTDNFRNEIE
                     VPEGKTAYWDLPSRWGITSKLTGSGRLVVYIPYVRSDLNGDWSAFSGRIDFVGRDVRL
                     NNAAARNLPLAEVNLGDGTYLMAASNGSSESSTAQTFTLGALSGTGTISGRNSLVIGS
                     KNTSTVFSGTISSGGGRLTKQGSGELDLRGANEYTGGTIVQQGSLIVSNSSGSATGTG
                     NVTVRSGATLTGRGSIAGTVQVLEGGILAAGSPTNVGSTLKLGRNLTMNPGSRMVFKQ
                     RLLYCDKLTVDGNISLNGTLELQIQGAGLKEGRSLALFTAGGTVSGAFEAIEPAVPGE
                     GLEWDLSRINEGILTVQAASALPSATSAEFRLIQSDNLPIYRINCEAATDPIKVEILS
                     LTGKLMENLESPAPDCLVDLNNYSAGLYLLRVSANHKTKVFKIVKKE"
BASE COUNT          808 a         1047 c         1076 g         1004 t
ORIGIN      
        1 gagcacgtga tttgtaatct cggggtcgtt ggttcgaatc cgacaagagg ctcaatcaag
       61 aggtcggcta aaaagcaatt tttagccgac ctcttatttc ttcccgatga ttacctgacc
      121 ttgtcagcag aaactgaatg ggcagaaaaa agaggatggc tttacagtca ccctagtctc
      181 tattccttct ttacaatttt gaatactttg gtcttatggt tggcgctgac cctgagcaag
      241 tacaagcctg ccgagtaatt attcagatcg accaggcagt cgggcgcagg gctttcgagg
      301 ttttccatga gtttgccggt caggctgaga atttccacct tgattgggtc tgtggccgct
      361 tcgcagttga tgcgatagat aggaaggttg tcgctttgaa taagccggaa ttcagcgctt
      421 gttgccgagg gtagggctga ggcagcctgt actgtcagga taccttcgtt gatacggctg
      481 aggtcccact ccaaaccttc gccgggaacg gcaggttcaa tggcttcgaa agcgccgctt
      541 actgtaccgc cggccgtgaa gagcgctaaa ctgcggcctt ctttcaggcc tgctccctga
      601 atttgcagtt ccagggttcc gttcaggctg atgtttccgt caacggtcag tttgtcgcag
      661 taaagcaggc gttgcttaaa taccatgcgg ctgcccgggt tcatcgtcag gtttcggcct
      721 aattttagcg tgctgcctac gttggtgggg gaacctgctg cgaggatgcc tccttccagc
      781 acctgtaccg taccggcgat gctgccccgt ccggtcagtg tggctccgga acgcaccgta
      841 acgttgcccg tgccggtggc acttcctgag ctattgctca cgattaggct gccttgttga
      901 acaatggtac cgccggtgta ttcgttggct cctcgcagat cgagttcacc actaccttgt
      961 ttggtaaggc gtccgcctcc gctactgatg gttccgctga aaacggtgga ggtattttta
     1021 ctaccgatga ccagactgtt gcggcccgaa atagttcctg taccgctcaa ggctcccaaa
     1081 gtaaatgttt gagccgtaga agattcgctg ctgccgttgg aagctgccat taggtaggtg
     1141 ccgtcgccca gattgacttc tgccagaggc agattgcggg ctgcggcgtt gttgaggcga
     1201 acatcacggc cgacaaaatc aatgcggccg ctaaaggcag accagtcgcc gttgaggtcg
     1261 gaacgcacat agggaatgta aacgaccagg cgtcccgatc ccgtaagttt tgacgtgatg
     1321 ccccagcgcg agggcaaatc ccagtaggcg gttttccctt cgggaacttc gatttcgttt
     1381 ctaaaattgt cggtgtcgga agttgtattt atgttgtgca tacggaaaat accgccctcg
     1441 aggcgtattt tggcggaacc catggaataa cggttggcat gggaggaacc cagcaaaagc
     1501 aggccgtctt tcaaaattac ccggccgaaa gtattgctgc cctgcaaagt aaggctgcct
     1561 ttccctttaa caatcagggt agtgttggct ccgctcatgt tgccgctgat cagcacactg
     1621 ttgttggatg cgggattgtt tattgtcagg gtgtctacca cctgcatatg ccggttggtc
     1681 gacatctggg acctggtttg cagggtggca ttgcgcataa tccagttttg gctgctgttg
     1741 gaagaggccc ctatggaact gggcaggcca ccgtctttga gagaggccac ttcgagtatg
     1801 ccgttctcta taatcacagg cccggtaaaa ctgttgtcgg tgttttgtat ggacaacagg
     1861 cccccgccgc ttttgctcag tccgccctcg ccgctgacga ctcccctacc gctcagactg
     1921 aaggcttctt cgttttcgaa catcagttgt cccaccggca tggtttcgtt gatcacaatt
     1981 tgtttgtaaa cagccgcgtc tgtaaaacgg acactgtcgc cgggcacaaa gatcgaagct
     2041 ccgttttgcc agttgaaatt gggagtggca aagtcccagg tgttgttttc gttacccatc
     2101 caaagaaccg aggccggttc gcgcgaagat ttgattttca gcgtaatgct gtgttctgct
     2161 atattaaggt cgtaaggaag gccttccagt ccgaatagcc tgaaatctcc ctttttagcg
     2221 ttggtgctgc cgctgaaagt aatcagggtc atttcctgtt ctttgggcag ttcccccgcg
     2281 gcttgaataa tcaggctgtt ttcgctgccg cttagcaaca ggtcgccctg aatctgcagc
     2341 aggtcgcaag cctcttcgct gatatcgaaa gccaggttgt tgcgacctct cagggacaga
     2401 ttgcccagaa tattcaaggt gtcgggcctg ccggcaaagc ccggttctat gcggccgcct
     2461 tcccggttga gcccttgttc cagaactact cctcctttta gcaccccgcg accccccaac
     2521 accccgcggg catcgatctg cacgggactg tccagactgc cgtccagcca gagcctgcct
     2581 tccgaaattc tggtgcttcc cgtgtaatcg tgttgtccgc gcagaacgat atcgccttgc
     2641 atggatttga tgagccgggt gctgccggta atttttccac tgccctccag cgtgtaatcg
     2701 gcatccttag gattcatgag gtagagcacc gatggtgaaa agtttccttc gatgttgaaa
     2761 gtgtcgttgc ctcccctcag gtcgaacatg accgacttgc cgtcggcata gtcggccgcc
     2821 tgctcctcac ggtccatcca gccggacccc gagtagtaga gatcggcctg ttgtaccgga
     2881 gctataggtg gtttgggcat gtcagcggcc atgtaaaaat ccacgtcggg agtttggtag
     2941 tagcctttgg ccgtagtttg caggcggtag gccgggttct gcatcaggtg gtagatgcga
     3001 tgattggttt gctcccaggt agatagcacc acaaatccgg tccaatcgct ccgcacgcag
     3061 atcagttcct cgcgccagtc gcccagcata tcgccccaaa aagccgccct tccggcgttg
     3121 atcgatttaa aataataggg tatgtattcg ttgtataaat tgggttgatc ccggtaaaac
     3181 gatttattcg acggattgta tacgtgaaaa gccagattgg gaccgccgct gcctatgccg
     3241 ctgatttccc agcgagccag gccgggcccc caccacaaag cttcggaggg gaaagtaccg
     3301 gcggctcctt ctatcagatc gcccttgcag tcgtacatag cgttgccgtt catggtcgac
     3361 cagtactgca tccccctgca cgtaacatcg aaagctccgc aaataccccg gcccacatcg
     3421 cccacggccg attggtagtt gcatttgatg attgcaccgg tggctgcatc gaacaggccc
     3481 atacccaagg ttttgggatt gttttgctgg atgatgaaat gttccagccc ggggcggtcg
     3541 gggtcgatgt cggccaggca atgtcggtcg ccgtgaacgg cgtcctgatg gcggttcaaa
     3601 atcgttccgt cgtgcttgag cacataacct ccttctacaa aattatcgcg tccgtcaccg
     3661 tccacatcgg ctgccctgac ctggtgaaat tcggcctgat taggccagaa gcggtaggcc
     3721 cagtccttga ttagcttgtt gttgcgatag ctaaaggcag cgtgggctcc gtaaaaccag
     3781 ccgttctcag cccggttttt gtacatatat accaacgagg gacgaatgcc gtcgaggtaa
     3841 ttgataatga aatggcctcc gatgtctttc cagttgtcgg tgcgttgatt gcgcagttct
     3901 gcataaaaag gcagatccac tttgtcgatg agcag
//
LOCUS       DAOSGL010000217         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_21143_length_3918_cov_3.644318, whole genome shotgun sequence.
ACCESSION   DAOSGL010000217 DAOSGL010000000
VERSION     DAOSGL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21143_length_3918_cov_3.644318"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PKY68_07000"
     CDS             complement(<1..1277)
                     /locus_tag="PKY68_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOH24817.1"
                     /translation="MKNCYAKNKFRSLLVLMLCLSTFTVSAQTLESKTITGTVSEAST
                     GKALVGARVQAYNNTRFVAMTDGEGRYTLEIPVYVNVLTVTLDGYALRHMALKGQTSG
                     VDLVLYSENFVPVLSPLTNVQNAGSAERFDLSSAISIESEIQSQLGAEVRTVSRSGIP
                     GAGSVMFVGGYNSLNVNAQPLIVLDDVILDQQYGRDLLHEGYYNNILSNLSVNDIESV
                     TVLKSGTSLYGAKGANGVLIIKTKRNKSMVTRIDACASAGVEFIPRLPRMMNAAQYRA
                     YASELLGTTDTKLTEFKFLKEDPDYYYYNQYHNETNWSDYVYREALTQNYSFGVQGGD
                     EVAKYNLSIGYVDAQSTLNYNDFDRLNLRFNSDISISDKLSALFDCSFSNVTRNLRDD
                     GVMPPEESNVFSPGFLSLIKSPFLSPYQYDTNK"
     gene            complement(1286..2908)
                     /locus_tag="PKY68_07005"
     CDS             complement(1286..2908)
                     /locus_tag="PKY68_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24818.1"
                     /translation="MKRSIFNIRYGLILLPILPLVWACSNDWDKHYGADPEVVPAQSL
                     WESIEARPDVLSDFTDLLRLCGYDKVLDADQVYTVWAPANGHFNVDSLRALINQGKKE
                     QVVTEFVKNHIARYSHPVSSLTDKHVLLLSKKTERLRSNEAKTSYLFGQTGIMNKNLL
                     SSNGILHIIDKANPFFFNIYEYLKTEPGLDSLKSFIYSFDELVFDPLLSVPYGVVDGK
                     TIYVDSVVTIENKLLEKIGLINHEDSTYWLLAPSNRAWSEAYDSIRQYFKYDFKVAKA
                     DSLEQVNTKMMMVRDLVFNANLQASPKDSLFSAQYRPLDKRYHVYQRPFDEGNIFSRI
                     TSEIVCSNGTVYTTDDWLYKPEQNFYQEIKVEAERTTGRDYHSSLCDIMDRAIKPEDD
                     INISNRRYIHLLPAGTSRNPWVSFEIPNTLSASYDIYCVFVPYKTYNPTHENVDKPTK
                     LKFSLTYNDEKGNSRTISCKDSTGNNIYVTDPSKLDSVLVLSDFKFPVANYGQEKNTV
                     LLKIETSITSKETAFYTREMLIDCICLKPHLK"
     gene            complement(2953..>3918)
                     /locus_tag="PKY68_07010"
     CDS             complement(2953..>3918)
                     /locus_tag="PKY68_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24819.1"
                     /translation="HFMPRTGYYNKLTTTLCLITSIIDPIEFYETMCPNTMFKISGTT
                     GLYMNRRVDNKFNIRGALIQPPGLSSTDQNAINGTYHYIDDILAYSKEVEEIVFNTRF
                     RFDTGALFPEFMTNNVRHNQDRQNYYFPNGYTEKAELTSETKFIYRYQHENYDCHQGD
                     EINFLGQYDITITLPPVPEGTYEIRLGYTALPTRGISQMYVDGEPTGIPVDLRIGATD
                     PKIGWIADERTDDNGVQNDKDMRNHKAMKAPDSMRRLNGNVYVVFRTESWLFRRILVT
                     KYLTQEPHTLRFRNVIDDLDAEFMFDYIEIVPKSVYDSEEGEDRH"
BASE COUNT         1019 a          848 c          900 g         1151 t
ORIGIN      
        1 ttcttgttgg tatcgtattg atacgggctt aaaaaaggcg acttgatcag actcagaaaa
       61 ccgggagaaa acacgttgga ttcttcggga ggcataacgc cgtcgtctct gaggttgcgt
      121 gtaacgttgc tgaaagaaca atcgaacaag gcgctgagtt tgtcgcttat actgatatcc
      181 gaattaaaac gcaggttaag acgatcaaaa tcgttgtaat tcaaagtact ttgcgcatct
      241 acgtagccaa tggataaatt gtatttggcc acttcgtctc cgccttgtac tccaaagctg
      301 tagttttgag tgagggcttc tcggtagacg taatcgctcc aattggtttc gttgtgatac
      361 tgattgtaat aataataatc ggggtcctct ttcaaaaatt taaattcggt aagtttggta
      421 tcggtagtac ccaatagttc cgaagcatag gcacgatatt gggcggcgtt catcatgcgg
      481 ggcagtctgg ggataaattc cacaccggca gaggcgcagg cgtcgatgcg cgtaaccatg
      541 ctcttgttgc gtttggtttt gataatgagc accccgttag cacctttggc accgtataaa
      601 gaagttccgc ttttgagtac tgtaacactt tctatgtcgt ttaccgacag gttcgataaa
      661 atattgttgt aatagccttc gtgcaacaaa tccctgccgt attgctgatc gagtatcaca
      721 tcatccagca ctatcagggg ctgggcgttg acgttaagag aattgtatcc gccgacaaac
      781 ataacggatc cggcgccggg aatacccgaa cgcgataccg tacggacttc agcccccaat
      841 tgcgactgaa tttcagattc tatactgatg gctgagctta aatcgaaacg ctcggccgat
      901 cctgcatttt gtacattggt gagtggactg agaaccggaa caaaattttc ggaatacaaa
      961 acaaggtcta cgcccgaagt ttgccctttg agtgccatat gtcttaaggc gtagccatcg
     1021 agagtaactg tcaggacatt tacatagaca ggtatctcga gtgtatatcg tccttcccca
     1081 tcggtcatgg ctacaaaccg ggtgttgttg taagcctgca ccctggctcc gaccagagct
     1141 ttcccggtag aggcttcact aactgtacct gttatggttt tgctttcaag agtttgtgcc
     1201 gataccgtaa aggtcgacaa gcacaacatt agaacaagca ggctcctgaa tttgtttttt
     1261 gcatagcagt ttttcatttc cgcaattatt ttaaatgagg tttcaggcaa atacaatcga
     1321 taagcatctc tcgggtataa aaagctgttt cttttgaagt tatactggtt tcaattttca
     1381 aaagaaccgt gtttttttcc tggccgtaat tggcaaccgg aaatttaaaa tcggacaaaa
     1441 ccaacactga atcgagtttt gaaggatccg ttacataaat attgtttccg gtactgtctt
     1501 tgcagctgat ggttctggaa ttaccctttt cgtcgttgta ggttagggaa aattttaatt
     1561 ttgtaggctt gtcgacgttt tcgtgcgttg gattataagt tttgtatgga acaaaaacgc
     1621 aataaatgtc gtaggatgcc gacaaagtgt tgggaatttc aaaactgacc cagggattgc
     1681 gcgaggtgcc ggccggcaac aaatgaatgt aacgccggtt ggaaatgttg atgtcgtctt
     1741 cgggttttat ggctctgtcc ataatatcac acagactcga atggtaatcc ctgccggtgg
     1801 tacgctctgc ttctactttg atctcctgat aaaaattttg ttccggcttg tataaccaat
     1861 cgtctgtggt atatacggtt ccattgctgc aaactatttc ggaggttatc cggctgaaga
     1921 tgttaccctc atcgaaaggc cgctgataca catggtatct tttatcgagc ggccggtatt
     1981 gggcgctgaa caatgaatcc ttgggtgagg cttgcaaatt ggcgttgaaa accaagtctc
     2041 tgaccatcat cattttggta ttgacttgtt ccaaagagtc ggctttggcc actttgaaat
     2101 cgtatttaaa atattgtctt atgctgtcgt aagcttcgct ccaagccctg ttactcggag
     2161 caagcagcca ataggtgctg tcttcgtgat tgataaggcc tattttttcg agcaatttat
     2221 tttcgatcgt gaccacagaa tccacataaa tagttttgcc gtcgaccaca ccataaggca
     2281 ccgacaacag gggatcaaaa acaagctcat cgaacgaata aataaaactt ttcaggctat
     2341 ccagtcccgg ttcggttttt aagtattcgt aaatattgaa gaagaaagga ttagctttgt
     2401 cgatgatatg taatatgcca ttggaactca gtaaattctt gttcataatc ccagtctgac
     2461 cgaataaata agaagtcttg gcttcgttcg aacgtaaacg ttccgttttt ttgctgagca
     2521 ataacacatg tttgtcagtc aggctcgata ccgggtggct gtagcgggcg atgtggtttt
     2581 tcacaaattc cgtcaccacc tgttcctttt tcccctgatt gatcagagca cgcaagctgt
     2641 ccacattgaa atgcccgttg gcaggtgccc aaaccgtata tacctgatcg gcatccaata
     2701 ctttgtcgta accgcacaga cggagcaaat ctgtgaaatc ggataataca tcgggccttg
     2761 cctctatgct ttcccataaa ctttgagccg gaaccacttc gggatcggca ccgtagtgct
     2821 tatcccaatc gttggagcaa gcccatacca aaggcaggat cggtaagagt attaatccgt
     2881 atctgatgtt aaatattgat ctcttcatat ttattctttc tatagttggc actttaagaa
     2941 agcgacaaca gtttaatgac ggtcttctcc ctcttcgctg tcgtaaacac ttttgggaac
     3001 aatttcaata taatcgaaca taaattctgc gtccaggtcg tcgattacat tacggaaacg
     3061 caaagtatgc ggttcctgag taaggtattt ggtcaccaga atgcggcgga acagccagct
     3121 ctccgttctg aaaaccacat aaacattgcc gttcagacgc cgcatactgt cgggagcctt
     3181 catagctttg tggttgcgca tatccttatc gttttgcacg ccgttatcat cggttctttc
     3241 gtcggcaatc cagccgattt tgggatcggt ggcgcctatg cgtaaatcca cgggaatacc
     3301 cgtaggttcg ccatccacat acatttgcga aatacccctg gtgggcagag cggtataacc
     3361 caacctgatt tcgtaggttc cttcgggcac cggaggcaaa gtaatagtga tgtcgtattg
     3421 gccgaggaaa ttgatttcat ccccctgatg acagtcgtaa ttctcgtgct gataacggta
     3481 tataaatttg gtctcgctgg tcaactcggc cttttcggta taaccattgg gaaaataata
     3541 attctgacga tcctggttat ggcgaacatt gttggtcata aattccggaa acaaagctcc
     3601 ggtatcgaaa cgaaaacgcg tattgaacac aatttcctcg acttcttttg aataggccaa
     3661 aatgtcgtca atgtaatgat aggtgccgtt gatagcattc tggtcggtgc tgctcaatcc
     3721 ggggggctgg atcaatgccc ctctgatatt gaatttattg tcgacacgtc tgttcatata
     3781 aagtcccgtt gtgccactga tcttgaacat ggtgttcggg cacatcgttt cgtaaaactc
     3841 tatggggtcg ataatggaag ttataaggca caaggtagtg gtcaatttat tatagtatcc
     3901 cgttcggggc ataaagtg
//
LOCUS       DAOSGL010000218         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_21482_length_3878_cov_3.786555, whole genome shotgun sequence.
ACCESSION   DAOSGL010000218 DAOSGL010000000
VERSION     DAOSGL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21482_length_3878_cov_3.786555"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..2102)
                     /locus_tag="PKY68_07015"
     CDS             complement(147..2102)
                     /locus_tag="PKY68_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH24820.1"
                     /translation="MNEQALNRHVLSNRIGLLMVLSALFFSCQSLTQPANPNPGPYSA
                     YLFAYFTGNNVEQEAVHYAISADGYNYLALNGNQPILNSSEISSTGGVRDPHILRGED
                     GKTFYMVLTDMVSAKGWSSNRAMVLLKSTDLINWQSSVVNIQNKYPNQEDLLRVWAPQ
                     TIYDKEAGKYMIYWSMMHKGGPDIIYYAYANEEFTDIEGEPKQLFFPSNGRSCIDGDI
                     IYKDGVYHMFYKTEGHGNGIKHASTTSLTSGQWTESEGYKQQTREAVEGSSVFKHLAD
                     NKYILMYDVYMKGSYQFTESDDLENFKIIDEAISMNFHPRHGSIIPITRAELKSLIEK
                     WGKPEGFPELNGNPVLEGYYADPDVLYSEKTGKYYIYPTSDGFDGWSGTYFKTFSSDD
                     LLNWTDEGIILNLETDVSWADRHAWAPCIIEKKMKKGYKYYYYFTAAQKIGVAVADQP
                     TGPFIDSGSALIDFKPEGINRGQEIDPEVFFDPVSGKDYLYWGNGYLAVVELNEDMIS
                     FKENSLKIITPDRTFREGVTVFYREGLYYFLWSENDTRDPDYRVRYGTSKSPLGPIEI
                     PEENLVIAKNPEAGIYGTGHNSVLQIPNKDEWYIVYHRFNWPAGIHMGRAAGFHREVC
                     IDKMEFGADGSILPVVPTHKSVEPQKE"
     gene            2284..2820
                     /locus_tag="PKY68_07020"
     CDS             2284..2820
                     /locus_tag="PKY68_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOH24821.1"
                     /translation="MKEKAKHILVPGAYLVLLLSFLAVGITAADRKSGNRQARSSQEV
                     PVLIEGIYYCDATQSRLYNGPYREYFENGDLKMEMYIVQGKPDGTYVVYYPNAKIKEV
                     RSYLNGVFNGIWRSYSEQGILLAQAEYLNGKKHGTWKIWDEQGTLRYEMYYDRGRKTG
                     IWFMWDETGKLISERKYN"
     gene            2835..3218
                     /locus_tag="PKY68_07025"
     CDS             2835..3218
                     /locus_tag="PKY68_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOH24822.1"
                     /translation="MKTKVINTINVLWMLLVALTLRAEPTAETKPATKMKSVAELSTE
                     TGAKIFSLSGKVYDLGSSESLAGASIEINGKVCYADLDGNFSIKSDTELNGKYLTVSL
                     ISYESKQIKLEKNQTQELEIALKQR"
     gene            3408..>3878
                     /locus_tag="PKY68_07030"
     CDS             3408..>3878
                     /locus_tag="PKY68_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOH24823.1"
                     /translation="MRRKLKNRLTKGLKTCGFLIVSMFLTFSLRAETGSISGKIFDAE
                     SGEQLIGAAVVIEGTILGAATDLDGEYHIQKVSPGTYTLTISYISYQTVTKENVVVEA
                     GKTTSLNVHLKPAEFLLTEMEVVARANRESENMMLLEQRKALLVTQAVSAHEMSR"
BASE COUNT         1050 a          825 c          985 g         1018 t
ORIGIN      
        1 caacggtttc aaaactcctt caccggaacg cgagcgtgaa atccttgcac atgtccgtcg
       61 tataggagag aagcttgcca atgcccactt ctaacaccat tccgtatgct cctatgggcc
      121 tcagaatcag caaacttatt tgtttattat tctttctgag gctcaacgct tttgtgcgtc
      181 ggaaccaccg gcaggatgga gccgtcggca ccgaattcca ttttgtcgat gcaaacttca
      241 cggtgaaatc ctgcggcacg tcccatgtgg atgccggccg gccagttgaa gcgatggtaa
      301 acgatatacc actcgtcttt gtttggaatt tgcagcaccg aattgtgtcc ggttccgtaa
      361 atgccggctt cgggattctt ggcgatcact aagttttcct cagggatttc gatgggcccc
      421 aaaggcgatt tggaggtgcc gtaacgaacc cggtaatccg ggtcgcgggt atcgttttcc
      481 gaccagagaa aataatagag tccttcacgg taaaacaccg tgacaccttc gcggaaagtc
      541 cggtcgggcg tgattatttt gaggctgttt tctttgaagg agatcatgtc ctcgttaagt
      601 tcaaccacag ccaggtagcc attgccccaa tacaaataat cctttccgga taccggatca
      661 aaaaagactt ccggatcgat ttcctggccc ctgttgatgc cttcgggttt aaagtcgatc
      721 agggcactgc ccgaatctat aaaggggccg gtgggctgat cggccacggc tactccgatt
      781 ttttgggcag cggtgaaata ataatagtat ttgtacccct ttttcatctt tttctcgatg
      841 atgcagggag cccaggcatg acggtcggcc cagcttacat cggtttccag gttgaggatg
      901 atgccctcgt cggtccagtt gagcaaatcg tcggacgaaa aggttttaaa gtaggtgccg
      961 ctccagccgt cgaagccgtc gctggtgggg taaatgtagt acttgcccgt tttttcggaa
     1021 taaagcacat cgggatcagc ataatagccc tctaacacag ggttgccgtt gagttcgggg
     1081 aatccttcgg gcttacccca tttttcgatc agggatttca gttctgcccg ggtaatggga
     1141 ataatggagc cgtgacgggg atggaaattc atcgagatgg cttcatctat gattttgaaa
     1201 ttttccagat catcgctttc ggtgaattga tagctgccct tcatatatac gtcgtacatc
     1261 agtatgtatt tattgtcggc cagatgtttg aataccgaag agccttctac ggcctcgcgg
     1321 gtttgttgtt tgtagccctc cgattccgtc cattgtcccg aagtcagcga ggtggtgctg
     1381 gcgtgcttga tgccgttacc gtggccttcg gttttgtaaa acatatggta aaccccgtct
     1441 ttgtagatga tgtcgccgtc tatgcaggaa cggccgttgg aggggaaaaa caattgtttg
     1501 ggttcgcctt caatgtcggt gaattcttcg ttggcatagg catagtagat gatgtcgggg
     1561 ccgcctttgt gcatcatcga ccaataaatc atgtatttgc ctgcctcctt atcgtaaatg
     1621 gtttgcggag cccatacacg caacaggtct tcctggttgg ggtatttatt ctgaatgttt
     1681 accacgctcg attgccagtt gatcaggtcg gtcgatttga gcaagaccat cgcccggttg
     1741 gagctccagc ccttggccga aaccatgtcg gtaagtacca tataaaaagt tttcccgtct
     1801 tcgccccgca agatgtgggg gtcgcgcact ccgccggtag aactgatttc gctcgaattg
     1861 agaataggtt ggttgccgtt cagggccaga tagttgtaac cgtctgcact gatggcgtaa
     1921 tgtacggctt cctgttcaac gttgtttccg gtaaaataag cgaacaggta agcgctgtaa
     1981 gggcccggat tgggattagc cggttgagtg agtgattggc aagaaaaaaa cagagcggaa
     2041 agaaccatca ataatccgat tctgttgctc aggacatgtc tgtttaaagc ttgttcgttc
     2101 atgggaaata atttgtcttg gtttgaaatc aataaaataa agcgtatcgc tgagtacaaa
     2161 aataagcaaa aaagttcagc aaactaaaaa atcggttttt atgacttttt gtaacaaaaa
     2221 cgtagcagga ttgataaggc tttttcacac cacagcttca tatttgcagc gtaaaagaac
     2281 gaaatgaaag agaaagcaaa acatattttg gtgccgggag cttatctcgt tttgctctta
     2341 tcttttttgg ctgtgggtat aactgcagca gaccgtaagt cggggaatag acaggcaagg
     2401 tcgtcgcagg aagtaccggt attgatcgag ggtatttatt actgtgatgc cactcagagc
     2461 agactctata acggccccta cagagagtat ttcgaaaatg gggacttaaa aatggagatg
     2521 tacattgtac aaggcaagcc cgacggcact tatgtggttt attatcccaa cgctaaaatt
     2581 aaggaagtcc gttcttattt gaacggagtt ttcaacggaa tatggagatc ttacagcgaa
     2641 cagggcattc tgctggctca ggccgaatac ctgaacggaa agaagcacgg tacgtggaag
     2701 atctgggacg agcagggaac actgcgatat gaaatgtact acgaccgggg ccggaaaacg
     2761 ggcatctggt ttatgtggga tgaaacagga aaacttatat cggaaagaaa atacaactaa
     2821 acaaaaaaac ggaaatgaaa accaaagtaa tcaacacaat caatgtttta tggatgttgc
     2881 tggtagctct gacgcttagg gcagaaccga cggctgaaac caagccggca accaaaatga
     2941 aatcagttgc cgagctcagc accgaaaccg gagcaaaaat tttcagtttg agcggcaagg
     3001 tttatgattt gggttcttcc gagtctctgg ccggagcgag catagaaatc aacgggaagg
     3061 tatgctatgc cgatttggac ggaaacttta gtattaaatc ggatacggaa ttgaacggta
     3121 aatatcttac cgtgagttta atatcttacg agagtaagca aatcaagctg gaaaagaatc
     3181 aaacacagga gcttgaaatc gctttgaaac agcgatgagt ttagcgataa gcaagaaaag
     3241 gattggatta ggattagcac gagggccttt cgtctttgag gggtcctctt tgtaatgttt
     3301 ttgtaacaca aagatgacaa ccttgtcact ggaacgccaa cagttcataa caaggcacgg
     3361 atacctttgc ccaagaaaaa tagaaagcag acagattgat ggtatcgatg cgcagaaagc
     3421 ttaaaaacag actcaccaag ggattgaaga cttgtggttt tttgattgtc tctatgtttc
     3481 ttaccttttc gctcagggcg gagacaggca gtatcagcgg taaaatattc gatgcggaat
     3541 cgggagaaca acttatcggg gccgccgttg ttatcgaagg cactatcttg ggtgcggcta
     3601 cggatttgga cggcgagtac cacatccaaa aagttagccc cggaacttat accttgacta
     3661 tttcctatat ttcttatcaa acggtcacaa aggaaaatgt tgttgtagaa gccggtaaaa
     3721 ccacatcttt aaatgttcat ctcaaacctg ccgaatttct cttaaccgaa atggaagtgg
     3781 tggcaagagc caatcgcgaa agtgaaaaca tgatgctgtt ggagcaacga aaagccttgc
     3841 ttgttaccca agcggtgagt gcacacgaaa tgtccaga
//
LOCUS       DAOSGL010000219         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_21605_length_3863_cov_5.045956, whole genome shotgun sequence.
ACCESSION   DAOSGL010000219 DAOSGL010000000
VERSION     DAOSGL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21605_length_3863_cov_5.045956"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKY68_07035"
     CDS             complement(<1..277)
                     /locus_tag="PKY68_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HOH24824.1"
                     /translation="MKKSVIWLLIGVMAMTFAALIALQIMYIQSLVKMQTDEFDGAVR
                     RSLSQVVYILEQDETRRYLENELEERDRKALNLRSSVISKVSPVPLPP"
     gene            complement(461..2620)
                     /locus_tag="PKY68_07040"
     CDS             complement(461..2620)
                     /locus_tag="PKY68_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH24825.1"
                     /translation="MKIYQTNEIKNLAIVGNAGAGKTTLAEAMLFEGGLVKRKGNINA
                     NNTVCDYFPVEHEYGYSVYSTVFHIEWKGKKLNLIDCPGSDDFVGGAVTALNMTDAAL
                     LVLNTQYGIEVGTQNSFRLADKYNKPIVFVLNQLDHEKAEFDRALEQLKEAFGSKVMP
                     LQFPVETGPGFNAIVDVLEQKLFTWKAEGGEPQVGEIPANLKAKAEAYHAALIEAAAE
                     NDEALMEKFFEDGTLNSEEIRKGIQLGLAAKGIFPVVCVCGGNGVGVSRLLDFMKDIA
                     PSVLDVPAPKNSGGVEVAPDANAPCSLYFFKTSVEPHIGEVCYFKVMSGKVKEGDDLQ
                     NTNRSSRERITQLFCTAGANRIKVEELQAGDMGATVKLKDLRTGNTLNAKGVEHKFDF
                     IKFPDPKYRRAVKPVNEADMEKMMTILNRMHEEDPTYIIEQSKELRQTIVHGQGEFHL
                     RTLKWIIENIDKLAITFAEPRIPYRETITKSARADYRHKKQSGGAGQFGEVHLIVEPY
                     TEGMPIPEMFKFGGQEYKVTNRDTQEYDLEWGGKLVFVNSIVGGTIDARFLPAILKGI
                     MGRMEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFMLAGRNAFSAAFRDAAPKILEP
                     IYDVEILVPGDRMGDVMGDLQGRRAVIMGMQSEKGLEKLSAKVPLKEMSNYSTSLSSL
                     TGGRASFTMRFASYELVPADVQDKLLKEYEAQQQEKE"
     gene            2733..>3863
                     /gene="hemW"
                     /locus_tag="PKY68_07045"
     CDS             2733..>3863
                     /gene="hemW"
                     /locus_tag="PKY68_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOH24826.1"
                     /translation="MSGSFGLYLHIPFCKSKCAYCDFYSLCGTEGKTVFLDCLSKESG
                     SEKQYIDGNQLSTIYFGGGTPSLLSANDYKLLFESIGREYDIGRCTEITLEANPDDLS
                     PAYLKSISGLPFNRISIGIQSFSDRELRALGRRHDASQGIEAVGRCREAGLNNISIDL
                     MFGIPEQNELSLQSNIEQALKLEVPHISVYMLSLEPGSQLYKDWQQGRFTEISDEEAE
                     KHYFLISDQLKQAGYDHYEISNFARPGFRAIHNASYWSGRPYLGLGPSAHSFNGRTRR
                     WNPASLEAYIQGIQNRNLIREEESLDLYTRYNELIMTHLRTSEGFSTDELLFLYGPEL
                     YDYCLEQARPLIAGGMLENTGGRIKLAYRAYFVSDSVIRALFW"
BASE COUNT         1001 a          952 c          845 g         1065 t
ORIGIN      
        1 tgggaggcag gggaacagga cttactttgg agataacaga cgagcgaaga ttcagtgcct
       61 tacggtcacg ctcctcaagt tcattttcaa ggtaacggcg tgtttcgtcc tgttccagta
      121 tgtataccac ctgagacagg ctgcgacgga cagctccgtc aaattcatcg gtctgcatct
      181 ttaccagcga ctggatatac ataatctgca aggctatgag cgcagcaaag gtcattgcca
      241 ttacaccaat caacaaccag atgaccgact ttttcatgga taatacttaa taatgaacga
      301 aaatacgtta aaaaattaat acatttcaat gattaaaaaa cattaacgtg tataaaaacg
      361 taaattaaca aaaaaaactt aagctcctga aaatgtaaca acccggaaca gtattttcga
      421 aaatcactgt cccgggctgt actaataatt taattaagtt ttattccttt tcctgctgct
      481 gtgcttcgta ttctttgagt agtttatcct gtacatcggc tggtaccagt tcgtaactgg
      541 caaagcgcat cgtaaaggaa gccctgcctc cggtgagcga actcagcgaa gtggaataat
      601 tcgacatttc tttcaaaggt actttggccg agagcttttc taaacctttt tcgctctgca
      661 tgcccataat gacggcgcgt ctgccctgaa ggtcacccat tacgtccccc atacgatcgc
      721 cgggtaccag aatttctacg tcgtagatgg gttccaaaat tttgggagca gcgtccctaa
      781 aagctgcact gaaagcatta cggccggcca gcataaacga aatttcgtta gagtctaccg
      841 ggtgcatttt accatcgtac acaataacac gaacgtcacg ggcataagag ccggtcagag
      901 ggccctgttc catgcgcccc atgattccct tgagaatggc cggcagaaaa cgggcatcga
      961 tggtaccacc cacaatactg ttaacaaaca cgagcttacc accccattcc agatcgtact
     1021 cctgagtatc gcgattagtg actttatatt cctggcctcc aaacttgaac atttcgggaa
     1081 taggcatgcc ttcggtatag ggttctacaa tcagatgcac ttcgccaaac tgtccggcac
     1141 cacccgattg ttttttgtgc ctataatcgg cccgggccga tttagtgatg gtttcgcgat
     1201 atggaatcct gggttctgca aaagtgatgg ccaatttgtc tatgttttct attatccatt
     1261 tcagcgtacg gagatgaaat tctccctgtc cgtgtacgat ggtttgcctt aattcctttg
     1321 attgttcgat aatataggta ggatcttctt catgcatacg attcaatatg gtcatcatct
     1381 tttccatatc ggcctcgttg acaggcttca ccgcacggcg gtacttagga tctgggaatt
     1441 taataaagtc aaacttatgt tcaacacctt tggcgttgag cgtattgcct gttctgagat
     1501 cttttaattt gaccgtagct cccatgtcgc cggcttgtaa ttcctccacc tttatcctgt
     1561 tggctccggc tgtacagaac aattgggtaa tacgttccct ggaactgcga ttggtatttt
     1621 gtaaatcgtc gccttccttt accttgccgc tcattacttt gaaatagcag acttcgccaa
     1681 tatgcggctc aaccgatgtt ttgaagaaat ataaactgca aggagcattg gcgtcgggag
     1741 cgacttcaac acctccgctg tttttgggtg ccggcacatc caaaacactg ggtgcaatat
     1801 ctttcatgaa atccaataaa cgactgactc ctacaccatt gccaccgcag acacatacaa
     1861 cggggaaaat tcctttggcg gccagaccca attgtattcc tttgcgaatt tcttcgctat
     1921 tgagggtgcc atcttcgaag aatttctcca tcaaggcttc gtcgttttcg gcagcagctt
     1981 caatcaacgc agcgtgataa gcctcggctt tggctttgag gttggccgga atttctccta
     2041 cctgaggttc tccgccctcg gctttccagg taaagagttt ttgctccagc acatccacta
     2101 tagcgttgaa tccggggccg gtttcaaccg ggaattgcag aggcataacc ttggagccga
     2161 aagcttcctt caactgctcc aaagctctgt cgaattcggc cttttcgtga tccaactgat
     2221 tcaacacgaa gacgattggt ttattgtact tatcagccag acgaaaactg ttttgagtac
     2281 ccacttcgat gccgtattgt gtgttcagga ccagcagagc agcatctgtc atgtttagtg
     2341 cagttacggc tccgcctacg aaatcgtccg atccggggca atcaatcaaa ttgagtttct
     2401 ttcccttcca ttcaatgtga aacaccgttg aataaacgga atatccatac tcgtgctcta
     2461 cggggaaata atcacatacc gtgttgttcg cgttgatatt ccctttgcgt ttgactaatc
     2521 caccctcgaa aagcatagct tcggcgagag tggttttccc ggctccggca tttcctacaa
     2581 tagccagatt tttgatttcg ttcgtctgat aaattttcat cttatttctc tgtttttaag
     2641 gttcaaaaaa gtggcgcaag atagaaataa ttctaaaaag aaacaacaac acaaccgaat
     2701 ttgaactaac tttgtctcag ccattttcca ctatgtccgg cagttttggt ctttacctgc
     2761 atattccttt ttgcaaaagc aaatgcgctt actgcgattt ctattccctt tgcggcacag
     2821 aaggtaaaac tgtttttttg gattgcctgt ccaaagaatc gggctccgaa aagcaataca
     2881 ttgacggaaa ccaactatcg acgatctact ttggaggcgg cactccttcg ctgctgagtg
     2941 caaacgatta caaactcctt tttgaaagca tcggcaggga gtacgacatc ggccgttgta
     3001 ccgaaattac gctggaagcc aatcccgacg atcttagtcc cgcttacctg aaatctattt
     3061 caggcttgcc cttcaacaga atcagtatag gcatccaaag tttcagcgac cgggaactcc
     3121 gggccctggg ccgcaggcat gatgcctcgc aaggcatcga ggcggtcggt cgttgccgtg
     3181 aggccggact gaacaacatc agcatcgacc tgatgtttgg tattccggaa caaaatgagc
     3241 tcagcctgca aagcaatatt gaacaagcgc tgaaacttga agttccacat atttcggtct
     3301 atatgctcag tctggagccc gggagccagc tttataagga ttggcagcaa ggacgcttta
     3361 ccgaaatctc cgacgaagag gccgaaaagc actattttct tataagcgat cagttaaagc
     3421 aggccggcta cgatcattat gaaatatcca actttgcccg gccgggtttc agagccattc
     3481 acaacgcctc ttattggagc ggccggcctt atctgggttt ggggccttca gcacattcat
     3541 ttaacggccg tacccgccgc tggaaccctg cttctctgga agcttacatc caaggcatac
     3601 aaaaccgaaa cttaattaga gaagaggaaa gcttagatct ttatacccgc tacaatgaac
     3661 tgattatgac tcacctaagg accagtgaag gattcagcac cgacgaactg ctgttccttt
     3721 acggccccga gttgtatgac tattgccttg agcaggcccg gcctttaata gcgggcggaa
     3781 tgttggagaa caccggcggc aggataaaac tggcttacag ggcttatttc gtttcggact
     3841 ccgtaataag agccttgttt tgg
//
LOCUS       DAOSGL010000220         3844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_21787_length_3844_cov_4.328319, whole genome shotgun sequence.
ACCESSION   DAOSGL010000220 DAOSGL010000000
VERSION     DAOSGL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21787_length_3844_cov_4.328319"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1982
                     /gene="ftsH"
                     /locus_tag="PKY68_07050"
                     /pseudo
     CDS             1..1982
                     /gene="ftsH"
                     /locus_tag="PKY68_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
     gene            2019..2846
                     /locus_tag="PKY68_07055"
     CDS             2019..2846
                     /locus_tag="PKY68_07055"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOH24827.1"
                     /translation="MKEFFARVVSGIAFVALLTFCILYGQHSFAILFALITGLCLWEF
                     YTLIEKQPDVKINKFMASLGGVYLFVAAYFYFSGILPSAIFLPWLGIMMYLSIAELFK
                     TNLKNMHSLAYTYFGQVYIGLAMVLLARMAFLSSTGESGTYNRVLLMSFFVFVWIADS
                     SAYVFGSLFGKHKLFERISPNKTWEGFIAELVITVAAGIPAALWFPDIFSKGEWMGFA
                     AVIVVFGLLGDLFESLIKRTIHVKDSGKLIPGHGGLLDRLDTVLMAIPAVSLYLLLI"
     gene            2926..3480
                     /locus_tag="PKY68_07060"
     CDS             2926..3480
                     /locus_tag="PKY68_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOH24828.1"
                     /translation="MDQQKENEILVLIRDPLTRSKAFSLLVSETSQSLYWQIRKMVLS
                     HDDANDILQNTYMKAWSNIEQFRGDSKISTWMFRIAINETYTFLNKERIKNQSNFTDM
                     EDIMVQNLRGDPYFSGDEAALKLQQAILTLPDKQRLVFNMKYFDDMKYEDMSEILGTS
                     VGALKASYHYAVKKIEAFMRADFE"
     gene            3549..>3844
                     /locus_tag="PKY68_07065"
     CDS             3549..>3844
                     /locus_tag="PKY68_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24829.1"
                     /translation="MSNNFNLDRPGNKEVFKVPEHYFENLQAEIQHRIEMESAPAQGI
                     KLFMNRVKPVLYMAAVFVLLLFSIRAVLNLTNTDNKQPIIGKTDGVEIEEFQEST"
BASE COUNT         1104 a          862 c          888 g          990 t
ORIGIN      
        1 atgagcgaaa tacaacgtcc caaagacact aaacccaagc ccaaattcaa catctactgg
       61 atctatgctc tgattctgat aggattgttt gctgttcagt tcatttcaag aaaggaagcc
      121 agcaaagaaa ttacctggac ggaaatggaa gagcatctgg taaacaacag agttaaagaa
      181 attctctgta tcaacaacaa aaaccaggtt cgcttaagta taaaagacga ggctctggta
      241 agcgtatttg gcgaaaatgt gaccaaagac aaagcgccgg tgttggtcgt ggaaattcct
      301 tcgcccgatc gtttttctga gtttatagaa aatttcaata atactcattc gccggatata
      361 gaggttaaat acgacaacac ctccaacacc tttgtgtctg tcctggtcag cctggctcct
      421 tttattttgc tcattgtcat ttgggttctt ataatgagga gaatggccgg cggaggcgca
      481 ggcggtgcgg gtggtgccgg aggcatcttc agcgtgggga aatccagagc cagattgttc
      541 gaaaaaggcg gagaaaaccc catcaccttc aaggacgttg ccggtctgag tgaagccaag
      601 caggaagtag aagaaatcgt ggccttccta aaaaatccgg acaagtacac cgagcttgga
      661 ggtaaatacc taagggtgcc ctgctgatag gccctcccgg aaccggaaaa acgctcctgg
      721 ccaaggccgt agccggcgaa gccgatgttc ccttcttttc gctttccggt tcggatttcg
      781 ttgaaatgtt tgtgggagtg ggtgcttcga gggttcgcga cctttttagg caagccaaag
      841 aaaaggctcc ctgcatcgtt tttattgacg aaattgacgc tgtcggaagg gcgcgcggca
      901 aaaatcccaa cataggtgct aacgacgaac gcgaaaacac cctgaaccaa ttgctcaccg
      961 aaatggatgg tttcggttcc aacagtggcg tcattatttt ggccgccacc aaccgggctg
     1021 acatattaga caaggcctta ctcagggccg gccgtttcga tcgccagatc aatgttgaat
     1081 tgcccgacat taatgagcgc cgcgaaatct ttaatgtaca cctgcgtccc ataaaaacag
     1141 acgacagcat cgattcggaa tttatggcaa gacagacgcc cggcttctcg ggtgccgaca
     1201 tcgccaacgt ctgtaacgaa tctgccctga ttgctgcacg caaaaacaaa acccatgtgc
     1261 aaaagcagga ttttctcgac gcagtagacc gcattatcgg cggcctggaa aaaaagaaca
     1321 aaataaccac cgccgacgaa cgtcgttcca ttgccataca tgaagccggt catgccacat
     1381 tgagctggct gcttgaattt gcccatccgc tcatcaaagt gaccattgtc ccgaggggta
     1441 aggccctggg tgcggcctgg tacctgccgg aagaacggca attgaccacc agggaacaaa
     1501 tgctggatga aatgtgttcc accctaggtg gcagggccgc cgaagaattg tttatcggaa
     1561 aagtgtcttc cggtgcgctc aacgacctgg aaagggtaac caagcaggct tatgccatgg
     1621 ttgcctattt tggtatgagc gataaattga acaatctctc ctacttcgac tccagtggcg
     1681 gttccgaatt cagtttttcc aagccttata gtgagaaaac cgccgaactc atagacaagg
     1741 aggtaaaagc tttggtagac gaacaatacc taagagcaaa aaaaatactg agcgaaaaca
     1801 aagatgggca tcaacagctc gccaatatcc tggtcgaacg cgaagttatc ttctccgaag
     1861 atatggaaca gatttttggc aaacgcaaat ggacatccag gatggaagag ttgatggagt
     1921 cgcaaaaaca acaagacgaa aaagaaaaaa ccgaatcaga ggatactccc ggcgaggaat
     1981 aaagaaattc agccttctta tccgattaat ctgctactat gaaagagttt tttgccagag
     2041 ttgtaagtgg aattgctttt gttgccctgc tgactttttg catactctat ggtcaacaca
     2101 gttttgccat tcttttcgcc cttattaccg gcctgtgcct ttgggaattt tacaccctga
     2161 ttgaaaaaca gcccgacgtc aaaataaaca aattcatggc cagcctgggc ggggtttatt
     2221 tgtttgtggc agcctatttt tatttcagcg gcatcctgcc gtccgccatt tttcttccct
     2281 ggttaggcat aatgatgtat ttatcaatcg ccgaactatt caaaaccaac ttgaaaaaca
     2341 tgcattccct ggcttatact tatttcggac aagtatatat cggcctggcg atggtgcttc
     2401 tggcccgtat ggcattcttg tcttccaccg gggaaagcgg aacttataat cgggttttgc
     2461 tgatgagttt ctttgtcttt gtttggattg ccgactcctc ggcttacgtc ttcggcagtc
     2521 tgtttggtaa gcacaaactt tttgaaagga tttcacccaa taaaacctgg gagggattca
     2581 tcgcggaatt ggtcatcact gtcgccgccg gtataccggc cgctctgtgg tttcccgata
     2641 tcttcagtaa gggcgaatgg atgggatttg cagccgtgat cgttgttttc ggcctgctgg
     2701 gcgatttatt tgaatcgctc atcaaaagaa cgatacatgt aaaagattcg ggaaaattaa
     2761 ttcccggaca cggaggtctg ctcgaccggc tggacactgt tttaatggct ataccggccg
     2821 tcagtcttta cctgctgtta atttagtggg tttagtaaag actccattaa actttttgaa
     2881 taaactatag tctgatacca ttgtgtagca ggaattcttt atcgaatgga tcaacaaaaa
     2941 gaaaacgaaa tccttgtatt gatcagagac cccctgaccc ggtcaaaagc ttttagcctg
     3001 ttggttagtg aaacatcgca aagtctgtat tggcagatta ggaaaatggt tctctcgcac
     3061 gatgacgcga acgatatttt acaaaacacc tacatgaaag cctggagcaa tattgagcaa
     3121 ttcagaggag attccaaaat atccacctgg atgtttcgta ttgccatcaa tgaaacatat
     3181 accttcctca ataaagagag gattaaaaac cagtcgaact ttaccgatat ggaagatatt
     3241 atggttcaaa acttgcgggg agacccctat ttttcaggag acgaagccgc actgaagctt
     3301 cagcaagcta ttttgaccct acccgacaaa caacgtctgg ttttcaacat gaaatatttc
     3361 gacgacatga aatacgaaga tatgtctgaa atcttaggaa cttcggtagg agctttaaag
     3421 gcctcgtacc actacgctgt caaaaagatt gaagcattta tgagagcaga ttttgagtga
     3481 tttttaaacc ttatttcatt atctttgtct aagatgatgt tattttccaa aaatacggag
     3541 gtacatttat gagcaacaat tttaatttag accggcccgg caataaagaa gtattcaaag
     3601 tccccgagca ttatttcgaa aacctgcaag cagaaattca gcaccgcata gaaatggaat
     3661 ctgctcctgc tcagggaata aagctcttta tgaaccgtgt gaagcccgtg ctttacatgg
     3721 ctgccgtatt cgtattgctc ttgttttcga tacgtgccgt actcaatctg accaacacgg
     3781 acaataagca acccataatc ggcaagacag acggagtaga aatagaagaa tttcaggaaa
     3841 gtac
//
LOCUS       DAOSGL010000221         3831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_21881_length_3831_cov_5.073623, whole genome shotgun sequence.
ACCESSION   DAOSGL010000221 DAOSGL010000000
VERSION     DAOSGL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21881_length_3831_cov_5.073623"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="PKY68_07070"
     CDS             complement(<1..1684)
                     /locus_tag="PKY68_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOH24830.1"
                     /translation="MKTSQTETEHRLSIRRTSHKNHLLIIGIVCSFILFVFYAFNSWR
                     AADKDLEKGALAYAQTIEVALNTVRLEFLQANEGVESSDYYRIIKDKLIELRRMNPNT
                     RTIYLLKQEEGKVIFLVDSEPANSIYYSSPGREYYDTDTAILKAFDRAEPVITDHHTD
                     SLGKWKSVVVPLRSFRNSAVYALLGIDYDVNRWKKLVLAEASEDIIYAFAVVLLFVSL
                     LLIMSKSDSLNKQEKLRQIAEQQAMALLHVVEQSDDIITVKDSDLRVFAANKAFARSA
                     GYDDFTEVIGKTDAEIYNIPPWEEPVRTYMDDERRARSLKPGEYLLREEELITHTGER
                     RTILTKKYPIYDTSGNVLFTSNISRDITERKKMEIELKNSKELAESASRAKSEFLSNM
                     SHEIRTPLNGVIGFTELLSNTPLNKMQKEYLDNAIVSANSLLGIISDVLDFSKIEAGK
                     LELDMVSTDVIRLVESAADIIKIQASHKDIELLLDVQPDLPRYMTLDPLRFKQVLVNL
                     MNNAVKFTHAGEVELKLNFIRLDENKGQLTVSVRDTGIGIKEADKSKLFKAFS"
     gene            complement(1691..>3831)
                     /locus_tag="PKY68_07075"
     CDS             complement(1691..>3831)
                     /locus_tag="PKY68_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH24831.1"
                     /translation="VKSMIAVALRNKNQTIGVIGFQMFGSYRRWTGADADMLISFSNF
                     MAEVLFKVEREKELIQARNLAEAASKAKSEFLSNTSHEIRTPLNGMIGFTELLRNTKL
                     NKIQLEYVENAIMSANTLMGVINDILDFSKIESGKLELDIVHSDIIQLVEKSSDIVKI
                     MASQKGLELLLNIQPDAPRYAYIDPIRLKQIMVNLLSNAVKFTTEGEVEICLKFKEID
                     EFEGQFTLAVRDTGIGIRDEDKSKLFKAFSQADSSVTRKYGGTGLGLVISNSLAEKMG
                     SKIDFTSEYGKGSVFSMSFVSRYERKQAEESMGSLPVKRVLIVDDNANNRKILEHNFM
                     YWGLDFSSAGSGKEALAVLEKDSDFDLILIDYNMPEMDGLTAVDAIREKKLLNPEKQA
                     IILLHSSSEDHTINERARKLQIWRTVIKPLKASDLLFYLKNLSPVSPATDLVKAPESP
                     LLREKPVDFTKQFCVLVAEDVKMNMMLVVQLIRKRFPNAKILEAENGKEVLELLENNL
                     PDLIFMDVQMPVMDGLDASRNIRTHNNPRVRSLPVVALTAGVSKSEQELCKLAGMNDF
                     IPKPIDKKILYQTISKYVASASGKWQAEVEESEKPSDISLHFNKNNLLDKIQNDKDLF
                     KQLMFQSLKEFDKDVNELLENIETGDPDRIKSGAHRLKGSAFNMEFTRLGNLAKVVEQ
                     NASDVFLLESEGEKLKTEWLTLKDIIANEI"
BASE COUNT          963 a          878 c          789 g         1201 t
ORIGIN      
        1 gtgaaaaggc tttgaacagc ttgcttttat ctgcctcttt tatgcctatg ccggtatcgc
       61 gcacgctcac ggtcaattgt cctttgtttt cgtcaaggcg tataaaattc agctttaatt
      121 ctacttcgcc tgcatgagtg aattttacgg cattgttcat caggttaacc aaaacctgtt
      181 taaaacgcaa gggatcgagt gtcatgtaac ggggtaagtc gggctgcaca tcgagcaata
      241 attcaatatc cttgtgcgag gcctgtattt tgattatgtc ggcggcgctc tctaccagcc
      301 ggatgacatc ggtcgatacc atgtcgagtt caagtttgcc ggcctcgatc tttgagaaat
      361 ccagtacgtc gcttataata cccagcaaag aattggccga tactatagcg ttgtcgaggt
      421 attctttttg catcttattc agaggcgtgt tcgacaacaa ttcggtaaaa cctatcacac
      481 cattaagagg ggtacggatt tcgtggctca tattggagag aaattccgat tttgctctgc
      541 tggcagattc tgcgagctct ttggaatttt tcaactctat ctccatcttt ttccgttcgg
      601 ttatatctcg cgaaatatta ctggtaaaca atacattgcc gcttgtgtcg taaatggggt
      661 atttcttggt caggatggtc cttctttcgc ctgtatgagt gatcaattcc tcttcgcgca
      721 gtaaatattc gcccggcttc aaactacggg ctcttcgttc atcgtccata taggttctga
      781 caggttcttc ccagggcgga atattgtaaa tttccgcatc ggttttacct ataacctcgg
      841 taaaatcgtc gtagccggca gagcgggcga aagccttgtt ggcagcaaat accctcagat
      901 ctgagtcttt taccgtgata atatcgtcgc tttgttctac aacatgaagc aaggccatag
      961 cctgttgttc ggctatttgg cgtagttttt cctgcttgtt cagagaatcg cttttactca
     1021 ttatcaataa aagactgaca aacagcaata caacagcaaa agcgtatatg atatcttctg
     1081 aggcttcggc taaaaccaat tttttccaac ggttaacatc gtaatcaata cccaataagg
     1141 catacacagc cgaatttctg aaacttctca atggcaccac cacgcttttc catttaccta
     1201 aactatctgt gtggtggtcg gtaattaccg gctcagcccg gtcgaaagct tttaaaatgg
     1261 ccgtatcggt atcgtaatac tcccgtccgg gcgaagaata gtatattgaa ttggcaggtt
     1321 ccgaatcgac taaaaaaata actttacctt cttcttgttt caacaaataa atggtacgcg
     1381 tattcgggtt cattctgcgg agttcaatca gtttatcctt aataatccga tagtagtcag
     1441 agctttctac tccctcatta gcctgcagaa attccagtct tactgtgttt aacgccactt
     1501 cgatggtttg agcataggcc aaagctcctt tttctaaatc cttatcggcc gctctccagg
     1561 aattgaatgc ataaaaaaca aacaaaataa aagaacagac tatgccaata atcaacaaat
     1621 ggtttttatg agaagtacga cgtatgctta gcctatgttc cgtttcagtt tgagatgttt
     1681 tcatggttac ttatatttcg tttgcaatta tgtcttttag ggtaagccat tctgttttta
     1741 gtttttcgcc ctcggactct aataaaaaaa cgtcggaagc attttgttcc actaccttgg
     1801 ctaaattacc caggcgcgta aactccatat tgaaggcact gcctttgagc ctgtgggctc
     1861 ccgattttat ccggtccgga tccccggttt ctatattttc caatagttca tttacgtctt
     1921 tatcaaactc cttcaaggat tgaaacatta attgtttaaa taaatctttg tcgttctgga
     1981 ttttatccaa gagattgttt ttgttgaaat gcaaagaaat atctgagggc ttttcgcttt
     2041 cttcgacttc ggcctgccat tttccgcttg cagaggctac gtacttcgat atggtctgat
     2101 acaggatctt tttatcaatg ggtttgggaa taaaatcgtt catccccgcc agtttgcaca
     2161 actcctgttc cgatttggaa actccggcgg taagggctac aacaggcaag cttcttacac
     2221 ggggattgtt gtgggtacgg atattgcgac tagcgtccaa accgtccatt acaggcattt
     2281 gcacgtccat aaagatcagg tccgggagat tattttcgag cagttcgagc acttctttgc
     2341 cgttctcggc ttccagtatc ttggcattgg gaaacctttt ccttataagc tgaaccacca
     2401 acatcatatt catcttgaca tcttcagcca ctaggacaca aaattgtttt gtaaaatcaa
     2461 ccggcttttc ccgaagaagg ggactttccg gagcttttac aagatctgtc gccggactta
     2521 ccggggacaa attctttaga taaaagagca agtcactggc cttaagtggc ttaatgactg
     2581 ttcgccagat ttgtaattta cgcgctcttt cgtttatggt gtgatcttcc gaggagctgt
     2641 gcaacaaaat aatggcctgc ttttcgggat tcagaagttt tttctccctt atggcatcaa
     2701 ctgccgttag accatccatt tcgggcatat tataatcgat caggatcaaa tcgaaatccg
     2761 aatccttttc gagcacagcc aaggcctctt tgccgcttcc tgcgctactg aaatccagcc
     2821 cccaatacat gaaattatgt tccaggattt tgcgattgtt ggcgttatcg tcaacaatca
     2881 gcaccctttt taccgggagg ctgcccatgc tttcttccgc ttgcttacgt tcgtagcggc
     2941 ttacaaaact catcgaaaaa acggaacctt tgccgtattc gcttgtgaag tcgattttgc
     3001 tgcccatttt ttcggccagc gaattagata taacaagtcc aaggccggtg cccccgtatt
     3061 tacgggtaac ggaagaatcg gcctgcgaaa aggctttaaa gagcttactt ttatcttcgt
     3121 cgcggatacc tatgccggta tcgcgcacag ccaaggtgaa ttgtccctca aattcgtcga
     3181 tttccttaaa ttttagacaa atttctactt cgccttcggt cgtaaattta acggcattgc
     3241 tcaacaaatt taccataatc tgcttaaggc gaataggatc gatataggca tacctgggcg
     3301 catcgggttg tatgttgagt aaaagctcca ggcccttttg actggccata atttttacta
     3361 tgtccgagga tttttctacc aattgtataa tatcgctgtg cacgatgtcg agttccaact
     3421 tacccgattc aatcttggaa aaatccagaa tatcgtttat cacacccatc aaggtgttgg
     3481 ccgacatgat ggcgttttcc acatattcca attgtatctt gtttagcttg gtgttgcgca
     3541 acaattccgt aaaacctatc atgccgttca gaggggtacg tatctcgtgg ctggtattgg
     3601 aaaggaattc cgattttgct ttactagccg cttcggccag atttctggct tgtatgagct
     3661 ctttttcgcg ctcgacctta aagagaacct cggccatgaa attgctgaaa cttatcaaca
     3721 tatcggcatc tgcgccggtc caccggcggt aactgccgaa catttgaaaa cctattactc
     3781 cgatggtttg gtttttgttt ctcaaagcaa cagcaatcat agatttgaca c
//
LOCUS       DAOSGL010000222         3825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_21933_length_3825_cov_4.694695, whole genome shotgun sequence.
ACCESSION   DAOSGL010000222 DAOSGL010000000
VERSION     DAOSGL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21933_length_3825_cov_4.694695"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKY68_07080"
     CDS             <1..409
                     /locus_tag="PKY68_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOH24832.1"
                     /translation="EGEPGGGPFLVYEEDGSTSLQILESSQIDMKDPEKAAMYHEGTH
                     FNPVDLVCGIKDYKGKKFDLRRYVDKNTGFISIKSKSGQEHKALELPGLWNGAMSDWI
                     TVFVEVPISTFNPVKTVNDLLREQHQDEDPSCS"
     gene            complement(382..1815)
                     /locus_tag="PKY68_07085"
     CDS             complement(382..1815)
                     /locus_tag="PKY68_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH24833.1"
                     /translation="MNLSEHIKDPLFILLSEAAEEMDVRCCLVGGYVRDIFLMVSSQD
                     IDIVTVGKGIELAELVAAKLGKSTRVNVFKNFGTAQLRYGDFELEFVGARRESYQRES
                     RKPIVEDGSLEDDLRRRDFTINAMAVSLNKADYGELIDPFDGRNDLRKKILRTPCDPD
                     LTFSDDPLRMMRALRFATTLQFNIDSATFEAIRRNKERISIVSAERIIDEIQKMMMAE
                     RPSKGFQLMDRSGLLDIILPELAALKGVETKDGKGHKDNFLHTLAVLDKVAAQSDNIY
                     LRWAALLHDVAKPLCKRWDDKLGWTFHNHNFLGEKKIPGIFRRLKLPMNEHMKYVQKL
                     VGLHMRPIALVEEEVSDSAVRRLLFDAGDDIEDLMILCEADITSKNPFKVQRFLENFQ
                     LVRRKMIDLEERDRIRNMQPPVSGEEIMDTFNLPPGKLVGEIKNSIKDAILDGLIPNE
                     YGAARDFMLQKGRELGLEPHEQEGSSS"
     gene            1898..2749
                     /locus_tag="PKY68_07090"
     CDS             1898..2749
                     /locus_tag="PKY68_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24834.1"
                     /translation="MSFRVFAYHVFVGFCLIIMQSCVSNKKDLPDTYIQKARAAIELR
                     QYQTAKNYIDSIRIVFPKDYDKIREGLQVMREIEFAEQKRTREYCDSLLNVRQSQFPE
                     KQKNFSFQRNKAYESVGYYVNNHQLHKNNYNRTFLQTKVDEKGRLIVTSYYSGPRKIN
                     HTRLRIYSSDGLFVETQDVPRDGALNYAFKDGDINYEIVRFNEKKLNGLINFILLNNG
                     GTLKVELIGDVNRTYNMRKEDKDALKAACELAAVLSDITRLLEEIRLAQAKMDYIRSK
                     QLSRAED"
     gene            complement(2746..>3825)
                     /gene="sprA"
                     /locus_tag="PKY68_07095"
     CDS             complement(2746..>3825)
                     /gene="sprA"
                     /locus_tag="PKY68_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOH24835.1"
                     /translation="NNSNKQVQYMIDGMPRTQTGGFSISTVALLTSFRGSSAKDGYYS
                     RYFEQFVQNRTVIAGRLQQRIEGLRYPNEGFLVGTELAGKAYDAQYGGIDLNSADVLI
                     PAFMAAYTGRSVHKGSLDIFPSIKAMLPNWTISYDGLSKLPFLAERFRSITLNHAYNC
                     AYNVTSYSSYNNFVGVEGEDFGFVRDVLTGNPLPSSMYDISSISLTENFSPLFNIQAT
                     LKNGMSVKTELRTSRSLNLSISGGQLVEAGQVQYVLGGSYKVADFHPWGILPNSKVKN
                     DMRLNADFSFKNSSALIRKIEENYSQATSGNKTVSLDLQADYVISRNLNLKFYYSLES
                     SIPLVSSYPVTTQDFGLSFRFSLTR"
BASE COUNT         1053 a          927 c          878 g          967 t
ORIGIN      
        1 cgaaggagaa cccggcggag gccccttctt agtgtacgag gaagacggca gcacttcatt
       61 gcagattttg gaaagttcac agatcgatat gaaggatccc gaaaaggcag ccatgtatca
      121 cgagggcacg catttcaatc cggtagatct ggtctgcggc atcaaagact ataaggggaa
      181 aaaattcgac ctcaggcgtt atgtggacaa aaacaccggc tttatttcca ttaaatcgaa
      241 aagcggacag gaacacaaag ctctggaatt accgggctta tggaacggag ccatgtccga
      301 ctggattacg gtttttgtcg aagtgcccat atctaccttt aatccggtta aaaccgtcaa
      361 cgacttgctc agggaacaac atcaggacga agatccctcc tgctcgtgag gttccagccc
      421 caattcacgg cctttttgaa gcataaaatc tctggccgcc ccatactcgt tcggaatcag
      481 accatcgagg atggcgtctt tgatgctgtt ttttatttct cccaccaatt tgccgggagg
      541 taagttgaac gtatccatta tttcctctcc gctcacagga ggttgcatat tacgaatacg
      601 gtcgcgctct tccagatcga tcattttccg gcgaaccaac tgaaaattct caaggaaacg
      661 ttgcactttg aacggatttt ttgacgtgat gtcggcctca cacagtatca tcaggtcttc
      721 gatatcgtcg ccggcatcga aaagcagacg acgaacagcc gagtcgctca cttcttcttc
      781 gaccaaagca atgggacgca tatggagacc taccagtttt tgcacatact tcatgtgttc
      841 gttcatgggc agcttgagcc ggcgaaaaat ccccggaatc tttttttcgc ccaggaaatt
      901 gtggttgtga aaagtccagc ccaatttgtc gtcccagcgt ttacagagag gtttggcaac
      961 atcgtggagc aaggcagccc agcgcaggta tatgttgtcg ctttgagctg ccactttgtc
     1021 caaaacggcc agggtatgta aaaaattatc tttatgtcct ttgccgtcct tagtttctac
     1081 ccctttgagg gctgccaatt cgggcaggat aatatccaac agtccggagc gatccatcag
     1141 ctgaaatcct ttcgaaggcc gttctgccat catcatcttt tgtatctcat caattatgcg
     1201 ttcggccgaa actatggaaa tacgctcctt attgcgacga attgcctcaa aggtcgcgga
     1261 atctatgttg aactgcaggg tcgtggcaaa acgaagggca cgcatcatgc gaagcggatc
     1321 atcgctgaat gtcaggtccg gatcgcaagg cgtacgcagg attttctttc gaagatcgtt
     1381 ccgaccgtca aaaggatcga tcaactcccc gtaatcggct ttgttcaggc tgacggccat
     1441 agcattgatg gtaaaatcgc ggcgccggag gtcgtcttcg aggctgccgt cctccacaat
     1501 gggtttacgg gattctcttt gataggattc ccgtctggct cccacaaatt ccaactcaaa
     1561 atcaccgtaa cgcagttgcg ctgtcccaaa attcttgaaa acattgaccc tggtagattt
     1621 ccccagtttg gcggccacaa gttccgccaa ttctatgccc tttcctactg taactatatc
     1681 gatgtcctga gagctgacca tgagaaagat atctctaaca tatcctccta ccaaacagca
     1741 cctcacatcc atctcttcgg ctgcctcaga taagagaatg aacagggggt ctttgatgtg
     1801 ttcacttaaa ttcacaaact acaactacta ttagccgaca aaagtacatt ttttttcatt
     1861 gtttactcta cttttgtgtg gactaaaatc aaaacttatg tcgttcaggg tttttgccta
     1921 ccatgtgttc gtcgggtttt gcctgattat catgcagtct tgcgtttcca acaagaaaga
     1981 cctgcccgat acttatattc aaaaggcccg ggctgccatt gaactgaggc aataccaaac
     2041 ggcaaaaaac tacatagaca gcatacgcat agttttcccc aaagattacg acaagattcg
     2101 cgaaggcctg caagtaatgc gtgaaataga atttgccgaa caaaagcgca cccgggaata
     2161 ttgcgacagc ttattaaatg tacgacagag tcagttcccc gaaaagcaaa aaaatttcag
     2221 ttttcagcgc aacaaggcct acgaaagcgt aggctattac gttaataacc accagttaca
     2281 caaaaacaat tacaaccgga cttttctgca gaccaaagtg gatgaaaaag gccgtctgat
     2341 tgtaaccagt tattattcag gtccaagaaa aatcaatcat accaggctgc gcatttattc
     2401 ctccgatggt ctttttgtgg aaactcagga cgtaccccgg gacggcgctt tgaattatgc
     2461 cttcaaagac ggagatatca actacgaaat tgtacgcttc aacgaaaaga aactcaacgg
     2521 cctgatcaat tttattttgt taaacaacgg aggcaccctc aaggtggaac ttatagggga
     2581 tgtcaataga acctacaaca tgcgcaagga agataaagac gccctcaagg cagcctgtga
     2641 gttggctgcc gtgctttcgg atattaccag gctgctcgaa gaaataagat tggcccaggc
     2701 caaaatggat tatatccgca gcaaacagct tagccgggcc gaagactaac gggtcagact
     2761 aaagcggaaa ctcaaaccga aatcctgagt cgttaccgga taagacgaca ccagcggaat
     2821 actggattcc agggaataat aaaacttcag attcaaattt cggctgatca cataatcggc
     2881 ctgcaaatcc agggaaactg ttttgttgcc cgaggttgcc tgcgagtaat tttcttcgat
     2941 ttttcgaatc agagccgagg aatttttaaa agagaagtcg gcattgagtc tcatatcgtt
     3001 ttttaccttg gagtttggca aaattcccca gggatggaaa tcggccactt tataactgcc
     3061 tcccaacacg tactgcactt gtcctgcctc gaccagctgt cccccgctga tgctcagatt
     3121 caggctccgt gatgtgcgca attcggtttt gacactcatt ccgtttttaa gggtagcctg
     3181 tatgttaaac aaagggctga aattttcggt caggctgatg ctgctgatat cgtacatgga
     3241 cgaaggcaga ggattgcccg taagtacatc cctgacaaaa ccaaagtcct ctccttcgac
     3301 gccgacaaaa ttattgtacg aagaataaga ggtgacattg taagcacaat tgtaggcatg
     3361 attcagagtg atcgacctga aacgctcagc caagaaaggg agtttggata agccatcgta
     3421 agatatggtc caattgggca gcatggcttt gatggagggg aaaatatcca ggctgccctt
     3481 gtgtactgaa cggccggtat aagcggccat aaaggcggga atcagcacat cggccgaatt
     3541 aagatcgata cctccgtatt gggcatcgta ggctttaccg gccagctcgg tgccgaccag
     3601 aaatccttcg ttgggataac gtaggccctc tatcctctgc tgcaggcggc cggcaataac
     3661 tgtccggttc tgcacaaatt gctcaaaata cctggaataa tagccgtcct tggccgaaga
     3721 acctctaaaa gaagtcagca aagccactgt cgatatcgaa aaaccaccgg tctgggtacg
     3781 gggcatgccg tctatcatgt attgcacctg tttgttgcta ttgtt
//
LOCUS       DAOSGL010000223         3825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_21935_length_3825_cov_3.754377, whole genome shotgun sequence.
ACCESSION   DAOSGL010000223 DAOSGL010000000
VERSION     DAOSGL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21935_length_3825_cov_3.754377"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKY68_07100"
     CDS             complement(<1..645)
                     /locus_tag="PKY68_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH24836.1"
                     /translation="MARPLAERMRPLTLDDYAGQKHLVGEGAILRKMIDSGTLSSFIL
                     WGPPGVGKTTLASIIAYKLECPFYSLSAVNSGVKDVRELIETAKNNRLFQQKPPILFI
                     DEIHRFSKSQQDSLLAAVEKGIVTLIGATTENPSFEVVTPLLSRCQVYVLKPLEEQDL
                     QELLDRALSKDPELKKRRILVEESGQLFRFAGGDASKLFNIIELLTQYQPEDPVI"
     gene            788..1867
                     /gene="serC"
                     /locus_tag="PKY68_07105"
     CDS             788..1867
                     /gene="serC"
                     /locus_tag="PKY68_07105"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOH24837.1"
                     /translation="MKKHNFYAGPSILSEYTIRQTADAILNFEGTGLSLMEISHRSKQ
                     FVAVCDQARALIKELLHVPEDFEVVFLGGGASLQFAMAPFNFLETKAAYLDSGYWASK
                     AIKEAKLFGEVQIVGSSKDDNYSHLPKNYQVPSDADYFYFCSNNTIYGTELHEDPHPG
                     VPVIADMSSDIFSRPIDFSSYGLIFAGAQKNLAPSGVTLVIVRKSMLDKVSRPIPTIL
                     NYKTHVENESMFNTPPVVPIYAALQTLLRYKELGGIEVIHDMNKQKAALLYEEIDTNP
                     LFKGTVTCKEDRSLMNVCFVMSEGYEHLEDEFDKYASEAGMVGIKGHRSVGGFRASIY
                     NAMPIASVQALVDTMRSFAQLKTQK"
     gene            1870..2790
                     /locus_tag="PKY68_07110"
     CDS             1870..2790
                     /locus_tag="PKY68_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH24838.1"
                     /translation="MKILVATEKPFAPVAVEGIREIIQGAGFGFELLEKYTDKNSLIE
                     VVSQADGVIIRSDIIDRQVIEAAGKLKIVVRAGAGYDNVDLEAASEKGICVMNTPGQN
                     ANAVAELAMGMMVYAVRNMFNGTSGTELKGKALGIHAYGNVGRNVARIAKGFGMEIYA
                     YDPYCKPEVIERDGVKVLTSVEDLYKKCQIVSLHIPATEETSKSINADLLGRMPKNAV
                     LVNTARKEVIDETSLMMLMENRIDFKYITDIMPAADAKFAEKFAGRYYSTPKKMGAQT
                     DEANINAGIAAAKQIVDFFKEGNQTFRVNP"
     gene            2797..>3825
                     /locus_tag="PKY68_07115"
     CDS             2797..>3825
                     /locus_tag="PKY68_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOH24839.1"
                     /translation="MVKIRAFKGWRPPRNLVEKVASRPYDVLNSEEARQEAANNEYSL
                     YRIIKPEIDFPPGTDEHEPKVYIKAKENFDLFRKKGWLVQDNKAMYYIYAQTMNNRTQ
                     YGLVVCASVEDYMNGLIKKHELTRRDKEEDRMKHVRVNNANIEPVFLAYPAQGALDKL
                     VAEHVRKEPVYRFTAPDGFGHQFWLVDDDESIGTITGCFEQVPALYIADGHHRTAAAA
                     LVGNEKKEQNPHHNGQEEYNFFLAVCFPDNQLQIIDYNRVVKDLNGHDKQSFLAALEE
                     DFIVESKGSQIYKPSRLHNFGLYIDREWYSLTAKPGRYDDKDPLGVLDVNISSRLILD
                     RILGIKDLR"
BASE COUNT         1087 a          866 c          900 g          972 t
ORIGIN      
        1 gattacaggg tcttcgggct ggtattgggt gagcagttca attatgttga acagcttgct
       61 ggcatcccca cctgcaaaac gaaacagttg gccgctctcc tcgaccaaaa tccggcgttt
      121 ttttagttcc gggtctttgc tgagggctct gtcgagtaat tcttgcaaat cctgttcctc
      181 gagaggtttt aacacataga cctggcagcg cgacagcagg ggagtgacga cctcgaacga
      241 gggattttcg gtggtagctc caatcagggt aacaataccc ttttcgacgg cagccagcag
      301 ggaatcttgc tgagatttac tgaagcggtg tatttcgtcg atgaaaagta tgggaggttt
      361 ttgttgaaac aagcggttgt ttttggctgt ttcgatgagt tcacgtacgt ccttcactcc
      421 tgagttgacg gcgctcaggc tgtaaaaagg gcactccagt ttataagcta taatgctggc
      481 cagagtggtc ttgcccacac cgggaggtcc ccagagaata aaagacgaca aagtgcccga
      541 atcgatcatt tttcgtaaaa tggctccttc gccaaccagg tgtttttggc cggcatagtc
      601 atccaaagtc agaggacgca ttctttctgc cagaggtcta gccatttgcg taagttactt
      661 gtcggtgata aaacgggaat cacaaaaata caaactaaat aaataaaact aaactcttgt
      721 cgtaagaaaa taaatcacta atttcgcggc gtttttctaa gcttcgattc attttatata
      781 ttttcctatg aaaaagcata atttctatgc aggaccatct atcctgagtg agtacactat
      841 taggcagacc gcagatgcca ttcttaattt cgaaggcaca ggcctctcgc taatggaaat
      901 ttcacaccgc agcaaacagt tcgtggctgt ttgcgaccag gcacgtgcct tgattaagga
      961 attactccat gttcccgaag actttgaagt cgtttttctg ggtggaggtg ccagtctgca
     1021 gtttgctatg gctcctttta attttttgga aaccaaagca gcctatctcg attccggata
     1081 ttgggcatcc aaggccatca aagaggcaaa attgtttgga gaagtgcaga tagtgggttc
     1141 ttccaaagac gacaactaca gccatttgcc caagaattat caggttccct cggatgcgga
     1201 ttacttctat ttttgcagca acaatactat ctacggtacc gaactgcacg aagatcccca
     1261 tcccggcgtt ccggtaattg ccgatatgtc ttcggatatt ttttcacgcc ccatcgattt
     1321 cagctcttac ggactgattt ttgccggagc acagaaaaac ctggctccct cgggtgtcac
     1381 tttggtaatt gtacgcaaaa gcatgctcga taaagtgagt cgtcccattc cgaccatcct
     1441 caactataaa acccatgtcg aaaatgaatc catgttcaat actcctccgg tggtgcccat
     1501 ttacgcagcc ttgcaaacct tgctccggta caaagaactc ggcggcatcg aagtcataca
     1561 tgatatgaat aaacaaaagg cagccctgtt gtacgaagaa atcgacacca atcccttgtt
     1621 caaaggtact gttacctgca aagaagaccg ttcccttatg aatgtatgct ttgtaatgtc
     1681 cgaaggttac gaacatctcg aggatgaatt cgacaaatac gcctccgaag ccggcatggt
     1741 gggcatcaag ggccaccgtt cggttggcgg tttccgtgct tccatttaca atgccatgcc
     1801 tatagctagt gtacaggcgc tggtagacac tatgagaagt tttgcacaat taaaaacaca
     1861 aaaataatta tgaagatatt agttgctact gaaaaacctt ttgctcctgt tgccgtcgag
     1921 ggcatacgtg aaataataca gggagccggt ttcggctttg aactgttgga aaaatacacc
     1981 gacaaaaaca gcctcatcga agttgtatcc caagctgatg gtgtaattat acgcagcgat
     2041 atcatcgacc gtcaggttat tgaagcagcc ggaaaattaa agattgtggt ccgtgccgga
     2101 gcaggttacg acaacgtaga tttggaggca gccagcgaaa aaggtatttg cgtgatgaat
     2161 acccccggcc aaaatgccaa tgccgtagcc gaattggcta tgggtatgat ggtatacgcc
     2221 gtgcgcaata tgttcaacgg tacttccgga accgaattga aaggcaaagc acttggcata
     2281 catgcctacg gaaacgtggg gcgtaacgtg gcccgcattg ctaagggttt cggtatggaa
     2341 atctatgcgt acgaccctta ttgcaagccg gaagtaatcg aaagagacgg ggtgaaggtc
     2401 ttgaccagcg tagaggattt atacaaaaaa tgtcagattg tttctttgca tattcctgct
     2461 acggaagaaa cttccaaatc cattaacgcc gatcttctgg gacgcatgcc caaaaatgcc
     2521 gtcttggtga atacggcccg caaagaggtg atagacgaaa cttctttgat gatgctgatg
     2581 gagaatagga ttgactttaa gtacattacc gatattatgc ccgcagccga tgctaaattt
     2641 gccgaaaaat ttgcaggccg ttattattcc actcccaaga aaatgggagc acagacagac
     2701 gaagccaata tcaatgcagg tatagcagcc gccaaacaaa ttgtagattt ctttaaagaa
     2761 ggtaatcaga cattcagagt caatccctaa agcaacatgg taaaaataag agctttcaaa
     2821 ggctggagac cgccacggaa tttggtagaa aaagtggcat cccggcctta cgacgtttta
     2881 aattccgagg aagccaggca ggaagctgcc aacaacgagt actccctgta tcgtatcatc
     2941 aaacccgaga ttgattttcc tccgggaaca gatgaacacg aacccaaggt gtacatcaag
     3001 gctaaagaaa attttgatct ttttcgtaaa aaaggctggt tggttcaaga caacaaagct
     3061 atgtattaca tctacgccca gaccatgaac aacaggaccc aatacggact ggtggtttgt
     3121 gccagcgtgg aagattacat gaacggcctt atcaaaaagc atgaattgac ccggcgcgac
     3181 aaagaagaag atcgtatgaa gcacgtcagg gtgaacaacg ccaatattga accggtgttt
     3241 ttggcttacc cggctcaggg agctctggat aagctggttg cagagcatgt tcgtaaagaa
     3301 cctgtttacc ggtttactgc tccggatggc tttggtcatc aattctggct tgtagacgac
     3361 gacgaaagca tcggcactat caccggctgt ttcgaacagg tacccgctct gtacattgcc
     3421 gacggacatc accgcacagc cgccgccgct ttggtgggca acgaaaagaa agaacaaaat
     3481 cctcatcaca acggacaaga ggaatacaat ttctttctgg ccgtttgttt tcccgataat
     3541 caattgcaga tcatcgatta caacagggta gtcaaagact tgaacgggca cgataaacaa
     3601 tcgtttctcg ccgctctcga agaagatttt atcgtcgaaa gcaaaggctc gcagatttat
     3661 aaaccttccc gtttgcacaa cttcggtctc tatatcgaca gggaatggta ttcgctcaca
     3721 gccaaaccgg gaagatacga cgacaaggat cctctgggcg tattggatgt gaacatttca
     3781 tcgcgtctga tattggatcg tattttgggc ataaaagatt tgcgc
//
LOCUS       DAOSGL010000224         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_22143_length_3803_cov_3.225720, whole genome shotgun sequence.
ACCESSION   DAOSGL010000224 DAOSGL010000000
VERSION     DAOSGL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22143_length_3803_cov_3.225720"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKY68_07120"
     CDS             <1..660
                     /locus_tag="PKY68_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOH24840.1"
                     /translation="IRGGGATVDLSCFDGYELASALAQFPLPLLSGIGHERDRSVADM
                     VAHTSVKTPTAAAELILSCLEQEDRKIEDMAASLLDMGQEAISAGVRQLQFYKERIVQ
                     LSSVQKNRGQLQIQKIESELRWAAREHLQQRKHRLDAAVQTIGLLSPQILARQLQRLD
                     VLKLKLEAYSPELQLKRGFSITLHQGRILRSAGGLPPGSLLETRLEDGRVFSKTIQKQ
                     T"
     gene            671..871
                     /gene="xseB"
                     /locus_tag="PKY68_07125"
     CDS             671..871
                     /gene="xseB"
                     /locus_tag="PKY68_07125"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOH24841.1"
                     /translation="MKSPISYQKAIEELESILQFLEQNEADVDQMTQKLKRATELIGF
                     CREKLHKTDLELQAIFKELEEK"
     gene            976..2040
                     /locus_tag="PKY68_07130"
     CDS             976..2040
                     /locus_tag="PKY68_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOH24842.1"
                     /translation="MKKLLLILTLSLALFSLAAKPVKNVIFLIGDGMGLNQVYAAALV
                     KGAPLYMMQDTKTGLILTYSYDNGITDSAAGGTALAAGVKTRNSMIGMGPDSVKVESI
                     LHQAARNGKSTGVMSTSAITHATPASYVANQVSREMQDEIALDFVKQGPDVFIGGGRR
                     YFEEREDGVDLTEQLKSKGYEVVYDLEHAEASGSAKLAAFLADDHLPDVISGREYLLP
                     EALNIALERLSRNKKGFFLMVEGSKIDMRCHVHDIMGSVAETLEFDETVKVALDFARK
                     NKNTLVIVTADHETGGLSVLQNAPYRKGEELIVHFSTGGHSGVPVPVYSYGPGSDNFT
                     GVLENTYFKATLARLMKLKP"
     gene            2042..2734
                     /locus_tag="PKY68_07135"
     CDS             2042..2734
                     /locus_tag="PKY68_07135"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOH24843.1"
                     /translation="MKAYALIMAGGAGQRMAAEIPKQFLLLHGRPILMHTLSAFYQYS
                     PAVKLVLVIPRQQQELWQKLCIQYSFDLPHLLAYGGKERFDSVRNGLQKIAGSEGFDA
                     ENSLIAVHDAVRPLVGVHLIETCYQTAERYGSAIPVTPLVDSLRRRDGEWSLAENRLD
                     FCAVQTPQVFNAKALLEAYRQPYHPSFTDDASVMEAAGHRIRLVEGHRKNSKITVPED
                     LLLAEYWLKQSK"
     gene            2713..>3803
                     /locus_tag="PKY68_07140"
     CDS             2713..>3803
                     /locus_tag="PKY68_07140"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH24844.1"
                     /translation="MVKAIEMRLSSLELTYLRGVGTARAELLKKELNIFNQEQMLYYF
                     PYKYIDRSRIYKISELNANMPYVQVSGRIISFKIIGEGRAKRLSAGFTDGSGLLELVW
                     FKGLKYTQDRYKLNQEYLVFGRPSQYGTRLSIAHPDIDPLNEQSLAFTRGLQAFYNTT
                     ERMKSSFMNSKAIQKIQISLFQSLTAPIPETLPSFLLEKLRLMSLDKSLREVHFPSDN
                     KSLEKARYRLKFEELFYLQLHILRYARKRDKKFKGYPFTRIGDYFNTFYHKYLPFELT
                     DAQKRVLKEIRINVGGQSQMNRLLQGDVGSGKTLVALMSMLMAADNGYQACMMAPTEI
                     LANQHYATLCGLLGDMPLKVALLTGSTRK"
BASE COUNT         1000 a          876 c          951 g          976 t
ORIGIN      
        1 atacggggag ggggagcaac ggttgatctt tcctgtttcg acggctacga actggcttcg
       61 gccctggccc agtttccttt gcctttgctg tcgggcatag ggcatgaacg cgatcgctcg
      121 gtggcggata tggtggcgca cacttcggtc aagactccta cggcagctgc cgagctgata
      181 ctgtcttgcc tggaacaaga agaccggaag atagaagata tggctgcaag cctgcttgat
      241 atggggcaag aagctataag cgcaggtgtt cggcaattgc agttttacaa ggaacgcatc
      301 gtgcagttgt cgtctgttca aaaaaaccgg gggcagctgc aaatccaaaa aatcgaatcc
      361 gaattacgat gggccgcacg agaacatctt caacaaagaa aacaccgttt ggatgcggca
      421 gtgcaaacca tcggcctctt gtctccccaa atacttgcac gtcagttgca acgactcgat
      481 gtgctgaaat taaaattgga agcttattct cccgaattgc agttaaaacg cgggttttcg
      541 atcactttgc accagggcag gattttgcgt tcggccgggg gacttcctcc gggtagcctg
      601 ctcgaaaccc gcctcgaaga cggccgtgtt ttttccaaga ccatacaaaa acagacctaa
      661 ttttttcaag atgaaatcgc ctatttctta ccaaaaagcc atcgaagaac tggaaagcat
      721 tttacaattt ctggaacaga acgaagccga tgtggatcaa atgactcaaa aattaaaaag
      781 ggccaccgaa ttgattggtt tttgtcgcga gaagttgcac aaaaccgacc tcgagttgca
      841 ggccattttt aaggaactgg aagaaaagta gtccggaatt tattgtttta ggtctgatta
      901 acactactta aagcaaagct tggttctttt ataagtacat ttgccggtat aaaataaatc
      961 cataaaaaaa ccacgatgaa aaagttattg ttgatattga cactgagcct cgcgctgttt
     1021 agtcttgccg ccaaaccggt taagaacgtc atttttttga taggcgatgg aatgggcctt
     1081 aatcaggttt atgctgcggc tctggttaaa ggagctcctc tgtacatgat gcaggacacc
     1141 aaaactggtc ttatcctgac ttattcctac gataacggta ttaccgattc ggctgccggc
     1201 ggtacggctc ttgcagcagg agtgaaaacc cgtaactcta tgataggaat ggggcccgac
     1261 agtgtgaagg tggaatccat tctgcaccag gctgcccgca atggcaagtc aaccggtgtc
     1321 atgtctacct ccgctataac ccatgctacc ccggcttctt atgtggctaa tcaggtatcg
     1381 cgggaaatgc aggacgaaat tgccctggat tttgtaaaac aaggcccgga tgtctttatc
     1441 ggcggaggac gcaggtattt cgaagaacgt gaagacggag ttgatctgac ggaacaactc
     1501 aaatccaagg gttatgaagt ggtgtacgat ctggaacatg ccgaagcttc cggctccgcc
     1561 aaactggcgg ccttcttggc cgatgatcat ctgcccgatg tgatttcggg acgcgaatat
     1621 ttgttgcccg aggctctaaa catagccctg gagcgtttga gccggaacaa gaagggtttc
     1681 tttttgatgg tagagggatc caagattgat atgcgctgcc atgtacacga cattatgggt
     1741 agcgttgccg aaactctgga gtttgacgag acggtcaaag tagccctcga tttcgcccgc
     1801 aaaaataaaa acacgctggt tattgttact gccgatcatg aaaccggtgg cttgagtgtt
     1861 ttacaaaatg caccttaccg aaaaggtgaa gagctgatag ttcatttctc aaccggcggc
     1921 cactccggag tccccgtacc tgtttattcc tatggccccg gctcggacaa ttttacaggc
     1981 gttttggaga atacttattt taaagctacc ttggctcggc tgatgaaatt gaaaccgtaa
     2041 aatgaaagct tatgcattaa taatggccgg tggggcggga cagcgtatgg ctgcagaaat
     2101 acccaagcaa tttttactgc tgcacggacg gcccattttg atgcatacac tcagcgcctt
     2161 ttatcagtac agtcctgccg taaaattggt gttggtcatt ccccggcaac aacaagagtt
     2221 gtggcaaaaa ctctgcatac aatattcttt tgatttgcct catcttttgg cctatggagg
     2281 aaaagaacga tttgattctg ttcgaaacgg cttgcaaaag atagccggat cggaaggttt
     2341 cgatgcggaa aatagcctta ttgccgttca cgatgccgtc agacctttgg tgggggttca
     2401 ccttatcgag acctgttatc agacggctga gcgttacggt tcggccattc ctgttactcc
     2461 tttggtagac agccttcggc gcagggacgg cgaatggagc ctggccgaaa accgactgga
     2521 tttttgtgcc gtgcaaacac ctcaagtttt caatgccaaa gctcttttgg aggcttacag
     2581 gcagccgtac cacccttctt ttaccgacga tgcttcggta atggaagctg ccggccaccg
     2641 tattcgcctg gtggaaggtc atcgtaaaaa cagtaagata acggtgcccg aagacctact
     2701 tctggccgaa tattggttaa agcaatcgaa atgaggctct cgtctttgga actgacctat
     2761 ctgcgtggag taggtactgc gcgggctgag ctcctgaaaa aggaattgaa tattttcaat
     2821 caggagcaga tgctgtatta ttttccgtat aaatacatcg atcgcagccg catatacaaa
     2881 atctcggagt taaacgccaa catgccctat gtgcaggtca gtgggcgtat tatttcgttc
     2941 aaaattatag gcgaaggcag ggcaaaacgt ttatcggccg gtttcaccga cggtagcggt
     3001 ttgctcgaat tggtgtggtt caagggtttg aagtacactc aggacaggta caaactcaat
     3061 caggaatact tagttttcgg caggccctcg caatacggca cccggctgtc gattgcccac
     3121 ccggacatag atccgctcaa tgagcagagc ctggctttta cccggggatt gcaggctttt
     3181 tacaacacaa cggaaaggat gaaatcgagt tttatgaatt ccaaagcgat tcaaaagatt
     3241 cagatttccc tgtttcaaag cctcacagcc cccattcccg aaaccttgcc cagctttttg
     3301 ctcgagaaac tgcgtttaat gtctctggac aagtccttgc gcgaggtgca ttttcccagc
     3361 gacaataagt cgctcgaaaa agccaggtac aggctgaaat tcgaggaact tttttatttg
     3421 cagcttcata ttttgcgtta tgcccgcaag agagacaaaa agtttaaggg ctatcctttt
     3481 actcgtatcg gcgattactt caatactttt tatcacaagt atctgccttt tgagcttacg
     3541 gatgcgcaaa aaagagtgct caaggaaata cgtatcaacg tgggcgggca gtcgcaaatg
     3601 aatcgtctgc tacagggaga tgtgggtagt ggtaaaaccc ttgttgccct gatgtctatg
     3661 ctgatggcag ccgataacgg ctatcaggct tgcatgatgg cacctaccga aattctggcc
     3721 aatcagcatt acgccacttt gtgcggactg cttggagata tgcccctaaa agtggccttg
     3781 ctgacaggct ccacccggaa aaa
//
LOCUS       DAOSGL010000225         3800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_22163_length_3800_cov_4.363418, whole genome shotgun sequence.
ACCESSION   DAOSGL010000225 DAOSGL010000000
VERSION     DAOSGL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22163_length_3800_cov_4.363418"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="PKY68_07145"
     CDS             complement(<1..1634)
                     /locus_tag="PKY68_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOH24845.1"
                     /translation="MNTLLFSERHIGISPEAEQKMLAKIGLNSLEELIDKTLPRDIRL
                     TQEPEPIEPMSEARFAEHIAGLAAQNKLFRSYIGQGWYGSITPAVIQRNVFENPGWYT
                     SYTPYQAEISQGRLEALLHFQTLVCELSGLPLSNSSLLDEATSAAEAAYMMFNLRSRE
                     QEKSGANKLFVDRGLFAPSLAVLKTRCSEQGIELVLGDYAAFRPDESFFGALLQYPNA
                     EGKVENYRSFADKVHKAGLKLAVAADLMALVMLEAPGKWGADIVFGSSQRFGIPMGYG
                     GPAAAYFAAREEFKRQMPGRIVGLSRDSRGKTAYRLALQTREQHIKREKATSNICTAT
                     ALMAIMSAFYAIYHGPKGLRDIATRIHGMTSYLNDALEVYGYEQENSSFFDTLKIKLP
                     EGLSSKSLRKLAEAAGINLYYYPDGKTLGLSLDETVDNRALNDLIEVFASAADNSAVP
                     VEEENWNSICALDDSLLRQEPVLQQSVFNSYHSETAMMRFIKQLERKDISLVHSMIPL
                     GSCTMKLNAASEMLPLSRPEFGAIHPMVPKEQAAGYL"
     gene            complement(1643..2869)
                     /locus_tag="PKY68_07150"
     CDS             complement(1643..2869)
                     /locus_tag="PKY68_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOH24846.1"
                     /translation="MINEGTLRRQHILKQLQLLQADACLLSSSVNLYYLNNRIYKGYC
                     YLPCEGEAIHFVRRPLDLKAEDIGFPAGETGQKSPGSQPDQAHFHLIRKVEQIPGILR
                     ELGMAPAENLMLEGDELSYAEWTRLQQCFPGSRLINGSHALRKARSVKTPYEQSLIRR
                     SAELHAASYRSIPRLFRPGMTDIELSIELERIMRLNGNLGLFRTYGADMDVFMGTVLT
                     GDNALVSSPYDFALGGAGHPSNPIGPNGSPILPGHSVMIDMAGNFTGYLDDLSRCYSY
                     GKLPEKAYKAHQVALELQDRLQERLKEGEICEDLYYFSIDFVASRGLADCFMGKNQQA
                     GFVGHGVGLVINELPVLAPKMKDVLEAGMVLAIEPKFVVEGVGAVGVENTFVVGKEHG
                     EKISLLEEEIIDLTNL"
     gene            3012..>3800
                     /locus_tag="PKY68_07155"
     CDS             3012..>3800
                     /locus_tag="PKY68_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOH24847.1"
                     /translation="MPQQRYIEIQGARVNNLKNISVQIPENAFTVITGLSGSGKSSLA
                     FDTLYAEGQRRYVESLSAYARQFLGRMNKPEVDFIKNIPPAIAIEQKVNTRNPRSTVG
                     TSTEIYEYLRLLFARIGKTYSPLSGEEVKKHRTKDVIEYVLKFPEGSRFLVLCPLNQP
                     KGRSLEDHLESFIKQGFSRIEYRGQILTIEDFLSQAGKISPSGIESLYLVIDRLLVQK
                     DDKLVQSRLADSIETAFYEGDHNCLIRMFTNEGSELRHFSDRFEA"
BASE COUNT          898 a          921 c         1011 g          970 t
ORIGIN      
        1 aggtagccgg ctgcttgttc tttgggcacc atgggatgga ttgcgccaaa ttccggccgg
       61 ctcagcggca acatttccga agcggcattg agcttcatgg tgcaggagcc caacggaatc
      121 atggagtgta ccagcgaaat atccttgcgt tccagttgtt ttatgaagcg catcatagcc
      181 gtttcggaat gataggaatt gaaaacggat tgttgcaata ccggctcctg acgaagcagg
      241 ctgtcgtcga gggcgcagat gctgttccaa ttctcttctt cgacaggcac ggccgagttg
      301 tcggcggccg aggcaaagac ttcgatcaag tcgttcaaag cccgattgtc gacggtttcg
      361 tccaggctca gtcccagggt tttgccgtcg gggtaataat aaagattgat tccggcagcc
      421 tcggccagct tgcgaagaga tttagagctg aggccttcgg gtaatttgat tttcaaggtg
      481 tcgaaaaagg agctgttttc ctgttcgtag ccgtacactt ccagagcatc gttcaggtag
      541 gaagtcatgc cgtgtatgcg tgtggcaata tcgcgcaaac ctttggggcc gtgataaatg
      601 gcatagaagg ccgacataat ggccatcagg gcggtggcgg tgcagatatt ggaagtggct
      661 ttctcccgtt tgatatgttg ttcgcgggtt tgcagggcca aacggtaggc tgttttaccc
      721 cggctgtctc tggatagtcc gacaatgcgg cccggcattt gtcgtttaaa ctcctcgcgg
      781 gcggcaaagt aggcggctgc cggtcctccg tagcccatgg ggattccaaa acgctgggaa
      841 gagccgaata cgatgtcggc tccccattta cccggggctt ccagcataac cagagccatc
      901 aggtcggcgg ccacggccag tttgagaccg gctttatgca ctttatccgc aaagcttcgg
      961 taattttcta ccttaccttc ggcattggga tattgcagca gggctccaaa gaaactctca
     1021 tcgggcctga aagcggcgta atcgcctaag accagttcaa taccttgttc cgaacaacgg
     1081 gttttgagaa cggccagcga tggggcaaac agaccccgat cgacgaaaag tttgttggct
     1141 cccgattttt cctgttcccg gctgcgcaga ttgaacatca tgtaggcggc ctcggcagcc
     1201 gaagtggctt cgtcgagcag ggaactgttc gagagcggca atccgcttaa ttcacaaacc
     1261 agggtttgga aatgcagcag agcttccagc cggccttgcg aaatttcggc ctgataggga
     1321 gtgtaagaag tgtaccagcc ggggttttcg aaaacattgc gctgaataac ggccggtgtg
     1381 atgctgccgt accagccttg tccgatgtaa gagcggaaca gcttgttttg ggcagccagc
     1441 ccggcaatgt gttccgcaaa acgggcttcc gacatcggtt cgattggttc gggttcctga
     1501 gttaggcgga tatcccgggg taaggttttg tctatcagtt cttcgagact gttcaagcca
     1561 attttggcga gcattttttg ttctgcttcg gggcttatgc cgatgtggcg ttctgagaaa
     1621 aggagagtat tcatattttt gattataagt ttgttaaatc gatgatttct tcttcgagca
     1681 agctgatttt ttcgccatgc tctttgccga caacaaaggt gttttcaacg cccacggctc
     1741 cgacgccttc gacgacaaat ttaggctcta tggcaagcac catgcctgct tccaaaacat
     1801 ctttcatttt gggagccaac acaggcaatt cgttgataac taagcctact ccgtggccga
     1861 caaagccggc ctgttgattc ttgcccataa aacaatccgc caagccccgc gaggcgacaa
     1921 agtctatgga aaaatagtat aagtcctcgc agatttcgcc ctctttcaat ctttcctgaa
     1981 ggcgatcctg cagttccagg gcgacttggt gggctttgta ggccttttcc ggaagtttcc
     2041 cataggaata gcagcgggaa agatcgtcca gataacctgt gaagttgccg gccatatcga
     2101 tcataacgct atgtccggga agtatggggc tgccgttggg tccaatgggg ttggagggat
     2161 gtccggctcc ccccagggca aaatcgtaag gagagctcac cagagcgtta tcgccggtca
     2221 acacagtacc cataaaaaca tccatatcgg ctccgtaggt tctgaacaaa cccaggttgc
     2281 cgttcaggcg cataatccgt tcgagctcta tggataattc aatatccgtc atgccgggcc
     2341 tgaagagccg gggtatgctc cgataggagg cagcgtgcag ttcggccgac cggcgtatca
     2401 aagactgttc gtaaggcgtt tttacgctgc gggcttttcg cagggcatgg ctgccgttta
     2461 tcagccggct gccgggaaaa cattgctgca accttgtcca ttcggcgtag ctcagttcat
     2521 cgccttcgag catcaggttt tcggcggggg ccattcccag ttctctgagg attccgggta
     2581 tctgctccac tttgcgaatc aggtgaaaat gtgcctgatc cggttgtgac ccggggcttt
     2641 tttgtccggt ttcgccggca gggaatccga tgtcttcggc tttcagatcg aggggacggc
     2701 gaacaaagtg aatcgcctct ccctcacagg gtaaataaca atagccttta tatattctgt
     2761 tgttgaggta atacaggttg actgagctgc ttaaaagaca ggcatcggct tgcaggagtt
     2821 ggagttgttt caggatatgt tgacgcctga gagtgccttc gttaatcatc agaataataa
     2881 cattttatat ttttttagcc cgcttgtgta aggagggctt tttaaaacgg caaaggtagg
     2941 cattaaaaaa aataattcct acctttgtac cctttttcaa aatcaccgaa aagaagctgt
     3001 tcaaccgacg tatgcctcaa caacgataca tcgagattca gggagctcgt gtcaataatc
     3061 ttaagaatat cagtgttcag attcccgaaa acgcttttac tgttataacc ggtttgtcgg
     3121 gttcgggcaa atcctctctg gctttcgaca ctctatatgc cgaaggccag cgtcgttacg
     3181 tcgaaagcct gtcggcctat gcccgtcagt ttttaggcag aatgaacaaa ccggaggtgg
     3241 attttattaa aaacatacct cctgccatcg ccattgaaca aaaggtaaat acgcgcaatc
     3301 cccgctcgac ggtgggcact tcgaccgaaa tatacgaata cctcaggttg ctgtttgccc
     3361 ggataggtaa aacttattca ccgctatcgg gcgaggaggt caaaaaacac cgaaccaaag
     3421 acgttatcga gtacgtgctg aaatttcccg aaggcagccg ctttttggtg ctctgcccgc
     3481 tcaatcagcc caaaggacga agcctcgaag atcatttgga aagctttata aaacaaggtt
     3541 tcagtaggat agagtatcgg gggcagatac taactataga agattttttg tcccaagccg
     3601 gtaagataag cccgtccggt attgaaagcc tctacctggt catagaccgc ttgttggtgc
     3661 aaaaggacga caaattggta cagagccgtc tggccgattc catcgaaacc gctttttacg
     3721 aaggagatca caactgtctg attcgtatgt ttaccaatga aggatcggag ctccggcatt
     3781 tttccgatcg tttcgaggcc
//
LOCUS       DAOSGL010000226         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_22554_length_3757_cov_4.311723, whole genome shotgun sequence.
ACCESSION   DAOSGL010000226 DAOSGL010000000
VERSION     DAOSGL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22554_length_3757_cov_4.311723"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PKY68_07160"
     CDS             <1..1282
                     /locus_tag="PKY68_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOH24848.1"
                     /translation="VANLIAQSAELPLFQVLSANPYLFNKAGSFIAQELGFGLAYGNA
                     YMEALTDRGIEAGKIAGKIRFHFGVSSDYFMEIAKFRAARLLWAKIVEAYLDGKACKS
                     AGKMKIHALTSEWNQSVYDMYVNLLRSQTEAMSAGIAGVDVLTVQPFDKAFKTPDEFS
                     ERIARNQQLLLKEEAGFSDVVDPSAGSYYIENLTMALAEQAWNYFLTVQEKGGFTAAL
                     RDAYIQDEVNASNQKRLGFIAQRREILLGTNQYPNYDETAAGRIETELVPACDCANAT
                     LRPLNFSRGASAFESLRLATEKSGKRPKVFLLTIGNLNMRLARAQFSGNFFACAGYEI
                     IDNLGFETIEEGFKAARAAKADIVVLCSSDEEYAQLAPEAYAALDKSKEILVIAGNPA
                     CSEELKALGITNFIHVRSNVLESLQTFNKQLNIH"
     gene            1287..3437
                     /gene="scpA"
                     /locus_tag="PKY68_07165"
     CDS             1287..3437
                     /gene="scpA"
                     /locus_tag="PKY68_07165"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOH24849.1"
                     /translation="MKPDFKNIDIYNPGIEERDPKTWEKAKGIQKNKKTPEGIELKSV
                     YTKADLEGMEHLHYAAGLVPYLRGPYSSMYVMRPWTIRQYAGFSTAEESNAFYRRNLA
                     SGQRGLSVAFDLATHRGYDADHPRVVGDVGKAGVSICSLEDMKVLFDGIPLNKMSVSM
                     TMNGAVLPIMAFYINAGLEQGAKLEEMAGTIQNDILKEFMVRNTYIYPPEFSMKIIAD
                     IFEYASKYMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGVNAGMDIDSFA
                     PRLSFFWAIGMNHFMEIAKMRAARMLWAKIVKQFNPKNPKSMALRTHSQTSGWSLTEQ
                     DPFNNVGRTCIEAMAAVLGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETNVC
                     KQVDPWAGSYYVETLTKELAEKAWALIQEIEELGGMAKAIETGIPKMRIEEAAARTQA
                     RIDSKTQTIVGMNKYRREKEDPIDILEVDNTAVRKQQIERLNQLRANRNETEVQKALD
                     AITLCVSTGEGNLLELAVEAARLRASLGEISYACEKVVGRYKAVIRTISGVYSSETKN
                     DADFQKACSLVDSFAKKEGRQPRIMIAKMGQDGHDRGAKVVATGYADCGFDVDMGPLF
                     QTPEEAAKQAVENDVHIMGVSSLAAGHKTLIPQVIEEMKKLGREDILVIAGGVIPPQD
                     YDFLYKAGVAGIFGPGTSVAKAACEIMKILLDEE"
     gene            3574..>3757
                     /locus_tag="PKY68_07170"
     CDS             3574..>3757
                     /locus_tag="PKY68_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOH24850.1"
                     /translation="MENLSLGLLLMVVGMPTVFLILLIIIYLGKGLIAFVNRFIPEEI
                     ILKNNDSESGIPDRIKR"
BASE COUNT         1044 a          930 c          882 g          901 t
ORIGIN      
        1 ggttgccaat ctgatagctc aaagtgcaga attgcctttg tttcaggtat tatctgccaa
       61 tccttatctt ttcaacaaag ccggttcgtt cattgctcag gaactgggct ttggactggc
      121 ttacggcaac gcctatatgg aagccctgac cgaccgaggt atagaagcgg gcaaaattgc
      181 cggtaaaatt cgcttccact tcggcgtaag ttccgattat ttcatggaaa ttgccaaatt
      241 cagggcagcc cggctgctct gggccaaaat cgtggaagcc tacctcgatg gaaaagcatg
      301 caaatcggcg ggtaagatga aaattcatgc cctcacttcg gaatggaacc aaagcgttta
      361 cgatatgtac gtgaacttgc tgcgcagcca gaccgaagcc atgtctgccg gcattgccgg
      421 ggtggatgtt ctcacggttc aacccttcga caaggccttc aaaactcccg acgagttttc
      481 ggaacgcatt gcccgcaatc aacaattatt gcttaaggaa gaagccggct tctcggacgt
      541 tgtagatcct tcggccggtt cctactatat agaaaatctc actatggccc tggccgaaca
      601 ggcctggaat tatttcctga ccgttcagga aaaaggcggc ttcactgccg cgctgcgtga
      661 tgcttacatt caggacgaag taaatgcttc gaatcaaaaa cgcctcggat ttatcgcaca
      721 acgtcgtgaa atacttctag gtaccaacca ataccccaat tacgatgaaa ccgctgccgg
      781 caggatagaa acagaacttg taccggcctg cgattgtgcc aatgcaacct tgcgcccgct
      841 gaatttcagt cgcggagcat ctgcttttga aagcttgagg ctggccaccg aaaaatcggg
      901 caaacggcct aaagtttttt tactgaccat cggcaatttg aatatgcgac tggccagagc
      961 ccagttctcg ggcaactttt ttgcctgtgc gggttacgaa atcatcgaca atctgggatt
     1021 cgaaaccata gaggaaggtt tcaaagctgc acgtgccgcc aaggcagaca tcgtagtgct
     1081 gtgttccagc gacgaagaat acgctcaact ggctcccgaa gcttacgccg cactggacaa
     1141 aagcaaagaa atcctggtta tcgccggtaa tcccgcatgc agcgaagaac tcaaagccct
     1201 gggcataacg aatttcatcc acgtacgcag caacgtgctg gaaagcctgc agacattcaa
     1261 caaacaactc aacatccact aagcaaatga aacctgattt taagaacatc gatatatata
     1321 accccgggat agaagaaaga gatcccaaaa cctgggaaaa ggccaagggc atacaaaaaa
     1381 acaaaaaaac acccgaaggc attgaattaa aatcggtcta taccaaagcc gatctcgaag
     1441 ggatggagca tctgcattac gctgccggac tggtccccta tttgagagga ccctacagct
     1501 ccatgtacgt aatgcgtccc tggaccatac gtcaatacgc cggtttctcc acagccgaag
     1561 aatccaacgc tttttatcgt cgtaacctgg cttcgggaca aagagggctt tctgttgcct
     1621 ttgacctggc tacacaccgc ggttacgatg ccgatcatcc ccgtgttgtc ggcgatgtgg
     1681 gtaaagccgg ggtttctatc tgttcgctag aagatatgaa agtacttttc gacgggattc
     1741 ctttgaataa aatgtcggtt tccatgacca tgaacggtgc cgttctgccc attatggctt
     1801 tttacatcaa cgccgggctg gaacaaggag ccaaactgga agagatggcc ggcaccatcc
     1861 aaaacgatat cctcaaggaa tttatggtgc gcaacaccta catctatccg ccggaattct
     1921 cgatgaaaat aattgccgac atctttgagt acgcctccaa gtacatgccc aaattcaatt
     1981 ccatttcgat ttccggctat catatgcagg aagcaggtgc cactgccgat attgaactgg
     2041 cctacaccct ggccgacgga ctggaatacc tcagaaccgg agtaaatgcc ggaatggaca
     2101 tcgacagctt cgctccccgc ctttcgttct tttgggccat tggcatgaac cattttatgg
     2161 aaatcgccaa aatgcgggca gcccgtatgc tttgggccaa aattgttaag cagtttaatc
     2221 cgaagaatcc caagtcgatg gctttgcgta cccactccca gacctccggt tggtcgctta
     2281 ccgaacaaga tcccttcaac aacgtgggac gcacctgtat tgaggctatg gcagcagtgc
     2341 tgggacatac ccaatctctg cataccaatg ctctggacga agccattgcc ctgccgacag
     2401 acttttcggc ccgcattgcc cgtaataccc aaatttacat tcaggaagaa accaatgtct
     2461 gcaaacaggt ggatccttgg gcgggttctt actatgtaga aaccctgacc aaagaactgg
     2521 ccgagaaagc ctgggcactg attcaggaaa tagaagaact gggcggtatg gccaaagcca
     2581 tcgagacagg tattcccaaa atgcgtatcg aagaagccgc tgcacgcact caggcccgca
     2641 tcgattccaa aacacagacc atcgtcggaa tgaataaata ccggcgcgaa aaagaagatc
     2701 ccatcgatat tctggaagtc gacaatacag ccgtgcgcaa acaacaaatc gaacgactca
     2761 accaactgag ggccaaccgc aacgaaactg aagtgcaaaa agccctggat gctatcaccc
     2821 tttgtgtcag caccggcgag ggcaatttgc tggaactggc cgtagaagct gcccgtctta
     2881 gggccagtct gggcgaaatc tcctatgcct gcgaaaaagt agtaggccgt tataaagctg
     2941 ttattagaac catatcaggc gtgtattctt cagaaaccaa aaacgatgca gactttcaga
     3001 aagcctgctc gctggttgac agttttgcca aaaaagaagg ccgtcaacca cgtataatga
     3061 ttgctaaaat gggccaggac ggccacgatc gcggagccaa agtggttgcc accggctatg
     3121 ccgactgcgg ttttgacgtc gatatgggcc ccttgtttca aaccccggag gaagctgcca
     3181 aacaagcggt cgaaaacgac gtgcatataa tgggtgtttc ctctctggct gccggacata
     3241 aaaccctgat tccgcaggta atcgaagaaa tgaaaaaact gggacgcgaa gacattctgg
     3301 ttatagccgg aggcgtgatt cctccacaag attacgattt cctgtacaaa gcgggggttg
     3361 ccggcatttt tggcccgggc acttctgtcg ctaaagccgc ttgtgaaatc atgaagattt
     3421 tattggacga agaatagact ccgaactttt tgaatttcaa aaaaatcttt tgattattta
     3481 gcctgttaca gccttgttat cttatttttt ttgagtaact tcgtgacgtt tttttctaaa
     3541 ctgggttgga gtcgttttta gtaagtaagc gttatggaaa atcttagtct tggtttgttg
     3601 ttgatggtag tcggaatgcc tactgtcttt ttaatccttc ttatcattat ttatttgggc
     3661 aaaggtctta ttgcttttgt caacagattt attcccgaag aaattatatt aaaaaacaac
     3721 gattccgaaa gtggaattcc cgatagaatt aaaagaa
//
LOCUS       DAOSGL010000227         3754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_22579_length_3754_cov_4.210327, whole genome shotgun sequence.
ACCESSION   DAOSGL010000227 DAOSGL010000000
VERSION     DAOSGL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22579_length_3754_cov_4.210327"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2396)
                     /locus_tag="PKY68_07175"
     CDS             complement(<1..2396)
                     /locus_tag="PKY68_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24851.1"
                     /translation="MRKLILLLVSGFALSFSLLAQVRIQGRITDQSGEALPGVNVVED
                     GTNNFAVSDVNGNYVLTDVNRNSTLIFSFVGMLREEIQVGNRTRIDVVMREDAVSLEE
                     VVAIGYGTVKKRDLTGAVQSIKSAEITQIPTGNMMQALQGRVAGLDIVRRSGRATSGV
                     EMVLRGKRSISGSNTPLFIIDGVIGNYEDINPNDVESIEIMKDASSTAIYGSAGANGV
                     IIVTTKKGQKGKAVVNVDSYYGVNGFLQFPPVRTGEDYIELRRQANLTTGAWARGDAD
                     SKLFSDPEWDAIQKNQWVDWFELGTRDGVLQNHSVSVSGGSENTIGYFSLNYYNEEGI
                     LKSDDISKYSFRANVEHQAKKWLKAGLNINGAFTDQNERKGQYFTRVLCLLPLGTPYN
                     EDGTINPFPLAGDTQLSPIADMAEDQYVNNRIALGVNPTSYLQINPIKGLTAKSVFSA
                     YLNFSRQGLFKGALSADGYGEGKSSAQIINGNRYNYKWENIVTYDFSIDDVHNFGITG
                     VTSWSENQGENASILGYNLNWNKYLFYNLAATDMQSRVATSSYVRTQMMSYAARVNYN
                     YMGRYFLTVSNRWDGASILAEGNKWDYFPAAALAWRISDEGFMQGLDKLDNLKLRLGY
                     GVTGNAGASAYATLNFGVAGSNLAFQETPAPYYMFSQNLANQVLDWEKSYNWNLGIDL
                     NMFKNRLNITTELYNTDTKDILFQRNLPASTGGYKAGNYSIWENVCETQNRGIEFEVN
                     TVNIKSNNFSWSTTLSLAANREKITKFVSEDPVTNGSTYLVEGYPIQSFYDYKYLGIW
                     "
     gene            complement(2863..>3754)
                     /locus_tag="PKY68_07180"
     CDS             complement(2863..>3754)
                     /locus_tag="PKY68_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOH24852.1"
                     /translation="YWGNPNLWYVKLNKDMVSYDLGIGDKGIVSVDMTPEAFGSQKGP
                     GDRVRTTYTEGPWLYKRNKTYYMVYASCGIPERISYSTSTSPEGPWTYRGVIMDRADH
                     LAFTIHPAIIDFKGNSYFFYHDQVLSKGQGFKRSVSVEQFDFNSDGSIPLIPSTREGV
                     KQALSVLKPFKKVEAETMAWSQGLKTGSDSLTGVYVTDINNGDFQLIRNVDFGRGAKV
                     FQASLASDSDGGLIEIRLGHPEGELIGTLEVLNTGGRQQWKTLSCELKKIKGVHDLCF
                     VFKGDEGALFNFDYWMFKRN"
BASE COUNT          924 a          880 c          867 g         1083 t
ORIGIN      
        1 tgccagatgc ccaggtattt ataatcataa aacgactgaa tgggatagcc ttcaaccaaa
       61 taggtgctgc cgttggtcac aggatcttcg ctgacaaact tggtaatctt ttcgcggttg
      121 gctgccaaag acagggtggt ggaccagctg aaattattgc ttttgatatt gacggtattc
      181 acctcaaatt ctatacctct gttttgggtt tcgcagacat tttcccaaat ggaataatta
      241 cctgccttgt aaccgccggt ggaagccggc agatttctct ggaacagaat atctttggtg
      301 tctgtgttat aaagttctgt agtaatgttc aaacggtttt taaacatatt cagatctata
      361 cccaggttcc agttgtagga tttttcccag tcgagtacct gattggccaa attttgtgaa
      421 aacatgtagt aaggagccgg ggtttcttgg aaagccaggt ttgatccggc cactccgaag
      481 tttagggtgg cataagccga agcaccggcg ttaccggtaa caccgtaacc caaacgaagc
      541 ttcaggttgt cgagcttgtc caggccctgc ataaatcctt cgtcgctgat tctccaggct
      601 agggcggcag cggggaaata atcccattta ttgccttcgg ccaaaatgga ggctccgtcc
      661 caacggttgg aaacggtcag gaagtaacgc cccatgtagt tgtagttgac acgggcggcg
      721 tacgacatca tctgggtgcg tacatacgaa gaggtggcaa ctctgctctg catatcggta
      781 gccgccaggt tgtagaagag gtatttgttc cagttgagat tgtagcccag tatgctggca
      841 ttttctccct gattttcgga ccaactggta actccggtta ttccgaaatt gtgcacatcg
      901 tcgatgctga aatcgtaagt gacaatgttt tcccacttgt aattgtatct gttaccgttg
      961 atgatctgcg ccgagctctt accttcaccg taaccgtcgg ccgacaaagc tcctttgaac
     1021 aatccttgtc gcgaaaaatt caggtaggcg ctaaacaccg atttggcagt gagacctttg
     1081 ataggattga tttgcagata agaagtggga ttaacgccca gagcaatccg gttgttgaca
     1141 tattggtctt cggccatgtc ggcaataggg ctcagctggg tatctccggc aagaggaaac
     1201 ggattgatgg ttccatcttc attgtagggc gtacccaggg gcagcaggca caaaacgcgg
     1261 gtaaagtact ggccttttct ttcgttctgg tctgtgaaag ctccgttgat attgagtccg
     1321 gcttttaacc attttttggc ctgatgttct acgttggcac ggaacgaata cttagaaata
     1381 tcgtcgcttt tgaggatacc ttcttcgttg tagtagttga gcgaaaaata accgatcgta
     1441 ttttcgctgc ctcccgacac ggaaacggaa tggttttgca aaactccgtc tcttgtgccc
     1501 agttcaaacc aatctaccca ttgatttttt tggatggcat cccattcggg atccgagaac
     1561 agtttggaat cggcatcacc tcttgcccag gctccggtgg tcaagttggc ctgacgcctc
     1621 aattcgatgt aatcctcgcc ggtacgaacg ggaggaaatt gaagaaatcc gttgacaccg
     1681 taataagaat caacattgac aacggctttg cctttttgac cttttttggt ggttacaata
     1741 ataactccgt ttgctcccgc cgaaccgtaa atggccgttg aggaagcgtc tttcatgatt
     1801 tcgatcgact ccacatcgtt gggattgatg tcttcgtagt ttccaatcac tccgtcaata
     1861 ataaacaagg gtgtattgct gccgctgatg gatctctttc cacgcaaaac catttcgacg
     1921 cccgaagtgg cccttcccga tctgcggact atgtcgagac cggctaccct gccctgcaga
     1981 gcctgcatca tgttgcccgt aggaatttgg gtgatttcgg cgctcttgat agattgaaca
     2041 gctccggtaa gatcgcgttt tttaacggtg ccgtaaccga tggccacgac ctcttcgaga
     2101 ctcacggcat cttctctcat tacaacgtca atgcgggttc tgttccccac ctgaatttct
     2161 tctcttaaca tcccaacaaa agaaaaaatc agggttgaat ttctgttgac atccgtcaaa
     2221 acatagttcc cgttgacgtc ggagacggca aaattgttgg tcccgtcttc gaccacattc
     2281 actccgggca aagcttcgcc ggactgatcg gtaatgcgtc cctgaattct cacttgagct
     2341 aacaaagaaa acgaaagagc aaaaccactc acaagaagta aaattaattt tctcatacct
     2401 tcacgattta aattaagaat tgatttaagg ttggctgctt gtttctctac ttgcctgaaa
     2461 ctgatataaa tgtagacttt atttaaacga tttagtctta taaaatgaga aggtatgtta
     2521 tatttgttca atttgcattt ttgaccctga aattcaacaa attgatcttt ttcctccttt
     2581 gtttttttat gcttttctac ttgctgttct gcgatttatc ttgttctgcg tcctaacaca
     2641 ggtaatactg tttgttaatt acgttaacac ctgcatcata aaaatcccca ccccgctggg
     2701 cttatgggcg tgcggggcgg ggtttaattt acccttttgt ggatttgttt ctatcgagcg
     2761 agacgggcgt caggtcaaaa gcggtaaaga ttaatttact ctattccgga ttgtttctgc
     2821 ctcggagcaa aatgttgcac gtatatacat gccaacaata aatcaattcc gtttaaacat
     2881 ccagtagtca aaattgaaca atgctccctc gtcgccctta aacacaaagc agaggtcgtg
     2941 aacgcctttg atctttttta attcgcagct cagcgttttc cattgttgcc gacctccggt
     3001 gttgagcact tcgagtgtgc cgataagttc gccttcggga tgacccagcc ggatttcaat
     3061 caggcctcca tcggagtcag aagccagact ggcctgaaat acttttgctc ctctgccaaa
     3121 atcaacattt cgtattagct gaaaatcgcc gttattgata tcggtaacgt aaaccccagt
     3181 caggctgtcg ctcccggtct ttaagccctg gctccaggcc atggtttcgg cttcgacctt
     3241 tttgaagggt ttcaagaccg acagagcttg ttttacaccc tcccttgttg aaggaatgag
     3301 cggaatactt ccgtcgctgt tgaaatcgaa ttgttcaaca ctaaccgaac gtttaaagcc
     3361 ctgccctttg gacaaaacct ggtcgtgata aaagaaatac gaatttcctt taaagtcgat
     3421 gatggcagga tgaatggtaa aagccaggtg atcggcccgg tccataataa cgccccggta
     3481 ggtccagggc ccttcaggcg acgttgaggt tgagtaggaa atgcgctcgg ggattccgca
     3541 ggaggcgtaa accatataat acgtcttgtt tcgtttgtat aaccaggggc cttcggtgta
     3601 agtggttcta acccggtctc cggggccttt ttgggaacca aaagcctcgg gggtcatatc
     3661 gacggaaaca atacctttat ctccgatgcc aaggtcgtac gaaaccatat ccttgtttaa
     3721 tttgacgtac cacaagttgg ggtttcccca gtat
//
LOCUS       DAOSGL010000228         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_22899_length_3715_cov_3.873224, whole genome shotgun sequence.
ACCESSION   DAOSGL010000228 DAOSGL010000000
VERSION     DAOSGL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22899_length_3715_cov_3.873224"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="PKY68_07185"
     CDS             complement(<1..1435)
                     /locus_tag="PKY68_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HOH24853.1"
                     /translation="MAKLIIYQVFPRYFGNNRRNSVSNGDIEDNGTGKFNDFSGKALN
                     AIKELGVTHIWYTGVLEHATQTDYSSYGIKSDPPELVKGKAGSPYAVKDYYDVSPDLA
                     VSVPDRIKEFKNLLRRTRQAGLKTIIDFVPNHLARTYCSDMAPNGVEDFGSRDRRDKA
                     FSPANNFYYLPGQSFRPPQEALIPGRKVQYTEKPAKVTGNDCFKAQPSVNDWYETIKL
                     NYGIDYQGGNLKYFDPVPDTWHKMKAVLEYWVKLGVDGFRCDMAEMVPLEFWAWVIPQ
                     IKKIKEVLFVGEVYNPGLYESFIRRGGFDYLYDKVGLYDTLRNVICEYAPTSAISDCW
                     KRVGPLQDRMLNFLENHDEQRIASDFFAQDPRKALPALIVSACMRNNPFMLYSGQELG
                     EKGMYREGFSGLDGRSSIFDYWCIDSLADWNNGGRFDGALLSAEQKELRNNYAKILNL
                     CSQEPALYRGLFFDLMYVNMDNPYFDFS"
     gene            complement(1437..2297)
                     /gene="fabD"
                     /locus_tag="PKY68_07190"
     CDS             complement(1437..2297)
                     /gene="fabD"
                     /locus_tag="PKY68_07190"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOH24854.1"
                     /translation="MKAYVFPGQGAQFTGMGKDLYDSFKEAKAYFELANKTLGFDLVQ
                     IMFEGSDDELKQTKVTQPAIFLHSVVAAKCLLDFKPDMVAGHSLGEFSALTAAGALAF
                     EDGLRLVYKRAMAMQAACEKNPSTMAAVLGATDELVEQVCASIESEIVVPANYNCPGQ
                     IVISGSFKGVELASEQLKAAGAKRVLPLKVGGAFHSPLMESAAAELQAAINQTKFSRP
                     CCPVYQNVDAKPYTDPVAIQKNLIDQLTSPVRWTQSVVAMLRDGAEEFIELGPGTVLQ
                     GLIAKIRANQ"
     gene            2489..>3715
                     /locus_tag="PKY68_07195"
     CDS             2489..>3715
                     /locus_tag="PKY68_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOH24855.1"
                     /translation="MKYFFPLLFFVLIACSGSSNRPYSQKMVESLSLRTFNNNRNTST
                     LQQADFSYVPGLVAKSVLKTFELYPDKRQYYESVKAYADRQLRDDPAAPLKISDDDID
                     AINAGKIFFDLYKQSVKDGDTESADKYKAAATFMYNKLKNEHTRILAPKPGAGGFIHK
                     GRYPDQMWLDGLYMGAAFYAEWEATFGESGNTESWSDIAYQFKTIHQYTWDSEKQLNY
                     HAWSASPTDSNSFWANQQEPFKGASKEFWGRGSGWYFAALVDVLELMPKEHVDYKALQ
                     DIYTQVALGLQRWQDKKSGCWFQLLQYDETTTADGLGDLVNGRRYNIGDLPNYLESSA
                     SAMFCYGMAKGVRLGLLDKESFMPTVEKAYQGLIDQFITLNPDQSINIIQSCASAGLG
                     PARNPSRTGTINYYLCG"
BASE COUNT          995 a          923 c          817 g          980 t
ORIGIN      
        1 tggagaaatc aaaataggga ttgtccatgt tcacatacat caggtcaaaa aacaagcctc
       61 tgtacaaagc cggttcttgc gagcataaat tcaagatttt agcgtaattg ttgcgcaact
      121 ctttttgttc tgcactcagc aaagcaccgt caaatcgtcc cccgttgttc caatcagcca
      181 gactgtctat acaccaatag tcaaaaatac tgctgcgtcc gtccaatccg ctgaaacctt
      241 cccggtacat gcctttttcg cccagttcct gccccgaata aagcatgaaa ggattgttcc
      301 gcatgcaggc agacacgatc agagccggca aggctttgcg gggatcttgg gcaaaaaaat
      361 cggaggctat acgctgttcg tcgtgatttt ccagaaaatt caacatacga tcctgcaagg
      421 ggccgactcg cttccagcaa tccgatatgg cgctggtcgg agcgtattcg caaataacat
      481 tgcgtaaggt atcgtaaagc cccaccttgt cgtacaaata atcaaaaccg cctcttcgta
      541 taaaagactc atacaatccc ggattgtaca cttcgcctac gaaaaggact tctttgattt
      601 tttttatctg cggtattacc caggcccaaa actccaaagg caccatttcg gccatatcgc
      661 aacgaaaacc gtctacgcca agctttaccc agtattccag tacggctttc attttgtgcc
      721 aagtgtcggg gaccgggtca aaatatttta gattccctcc ttgataatca ataccgtaat
      781 tcaattttat ggtttcatac caatcgttga ccgagggttg agccttaaaa caatcgttgc
      841 ctgttacttt ggcaggtttt tcggtgtatt gcacctttct accgggaatc agcgcttcct
      901 ggggaggccg aaaactttgt ccgggtaaat aataaaaatt attagcaggc gaaaaggctt
      961 tgtccctgcg atccctggag ccgaaatctt ctacaccgtt gggagccata tcggaacaat
     1021 aagtacgggc taggtgattg gggacgaaat cgataatagt tttcaggccg gcctggcggg
     1081 ttcgcctaag caggttttta aactctttga ttctgtcggg taccgatacg gccaaatcgg
     1141 gacttacatc ataataatcc tttacggcat agggcgagcc ggccttaccc tttaccagtt
     1201 cgggaggatc ggactttatg ccgtagcttg aataatcggt ttgagtagca tgttccagaa
     1261 ccccggtgta ccaaatatga gtcactccca attctttgat ggcgtttaag gctttgccgc
     1321 taaagtcatt aaacttcccc gtcccgttat cttcgatgtc tccgttgctt acagagtttc
     1381 tgcgattgtt tccaaaatag cgaggaaaca cctgataaat aattagttta gccatattat
     1441 tgattggctc tgattttggc aatcaatcct tgtaatacag tgcccggacc cagttcaata
     1501 aattcttcgg caccatctct caacatagct accacgcttt gtgtccaacg cacgggagaa
     1561 gttaattgat caatcagatt cttttgtatg gcgacgggat ctgtatatgg cttggcatcg
     1621 acgttttgat acaccggaca acaaggccgg ctgaactttg tttgattgat ggctgcctgc
     1681 aattccgctg cagcactctc catcaatggg gaatgaaaag ccccgccgac tttgagaggc
     1741 agcacgcgct ttgcaccggc ggcttttagc tgctctgagg ccagctccac tcctttgaag
     1801 ctgcccgaaa ttactatttg tccggggcaa ttgtaattgg caggaacaac tatctccgat
     1861 tctatggagg cacagacctg ttctaccaat tcgtccgtgg cgcccaacac ggcagccatg
     1921 gtagaaggat ttttttcgca agcggcctgc atggccatgg ctcttttgta aaccaatctc
     1981 aaaccgtctt caaaagccaa agctccggct gccgtcagtg ctgaaaattc ccctaaagaa
     2041 tgtccggcca ccatatcggg tttgaaatcg agcaaacact tggccgctac taccgaatgc
     2101 aaaaagatag caggctgagt caccttagtc tgcttcaact catcgtccga accttcgaac
     2161 ataatttgca ccagatcaaa acccagggtt ttgttggcca attcaaagta cgcttttgct
     2221 tctttgaaac tgtcgtacag gtctttgccc ataccggtaa actgagctcc ctgtccggga
     2281 aatacgtatg ctttcataat ttggtcgtaa taattcgggg caaaaataca taattttaaa
     2341 ggaatgcaga ttttttcgta tttttactgc ccggtagaat tgcttgcttg tatgttgaat
     2401 catccaattt atgaccgggc ttagaggcaa acaaaaatat taactacgta gatttcaatc
     2461 attacacact tttagaatga agcatattat gaagtatttc tttccccttt tattttttgt
     2521 cctgattgcc tgcagcggaa gttcaaaccg tccctattcg caaaaaatgg tcgaatcgct
     2581 tagtctgcgc acattcaaca acaacaggaa caccagtaca ttacaacaag ctgatttcag
     2641 ctatgttccc ggcttagtcg caaaaagtgt attgaaaacc tttgaattat atcccgataa
     2701 aagacaatac tacgagagcg tcaaagccta tgccgaccgc caactccggg acgatcctgc
     2761 tgctccgcta aaaatcagcg acgacgacat tgatgccatc aacgccggca aaatcttttt
     2821 tgacctgtat aaacagtccg tcaaagacgg cgatacggaa agcgccgata aatacaaagc
     2881 ggctgctacg tttatgtaca ataaactaaa aaacgaacat acgcggattc tggccccaaa
     2941 accgggagcc ggaggcttta tacacaaagg acgttatccc gaccagatgt ggttggacgg
     3001 attgtatatg ggagccgcct tttatgccga gtgggaagcc actttcgggg agtccggaaa
     3061 tactgagtcc tggtccgaca tcgcctacca gttcaagacc atacaccaat acacctggga
     3121 cagcgaaaag cagttgaatt accatgcctg gtctgcttct cccaccgact ccaattcttt
     3181 ctgggccaat cagcaggagc ctttcaaggg agcctcgaag gaattctggg gcagaggttc
     3241 cggctggtat tttgctgcac tggtcgacgt gctcgaacta atgcccaaag aacatgtcga
     3301 ctacaaggcc ttacaagata tttatacgca ggtggctttg gggctacaac gctggcagga
     3361 taaaaaatcc ggctgttggt ttcaactgct gcaatacgac gaaactacca cagccgacgg
     3421 cctgggcgac ctggtaaacg ggcgacgtta caatatcgga gacttaccta attatctgga
     3481 atcatcggct tctgctatgt tttgttacgg catggccaaa ggtgtacggc tgggcttgct
     3541 cgacaaagag agctttatgc ccacggtcga aaaagcctat caaggcttaa ttgatcagtt
     3601 tatcaccctc aatccggacc aaagcatcaa tatcattcag agttgtgcct ccgcagggct
     3661 tgggccggcc cgtaatccct cacgcaccgg taccataaat tattaccttt gcgga
//
LOCUS       DAOSGL010000229         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_23262_length_3673_cov_4.646766, whole genome shotgun sequence.
ACCESSION   DAOSGL010000229 DAOSGL010000000
VERSION     DAOSGL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23262_length_3673_cov_4.646766"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..195)
                     /locus_tag="PKY68_07200"
     tRNA            complement(120..195)
                     /locus_tag="PKY68_07200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Gly,seq:ccc)
     gene            complement(257..1036)
                     /locus_tag="PKY68_07205"
     CDS             complement(257..1036)
                     /locus_tag="PKY68_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HOH24856.1"
                     /translation="MKKPAFKNIALLCGSILALLGMSCTQKEDIRLFVAGDSTAQSYR
                     VEQTLMRGWAQYLELFFDERVQVENRSMAGRSSKSFREEGRWNKILEDLRKGDVVFIQ
                     FGHNDTSTKPERHASPADYRQNLRRFVQEVREREATPVLLTSIVLCTFNDSGQLVDRR
                     LKEYPQIVRDLAAEEKVILIDMFRLSFDLLQELGPEEARKLYMFLEPGEDPSRPQGSN
                     DDTHLRERGAKQLAALVAEEIRRARIKPMYRYLLDKEEWPQ"
     gene            complement(1099..2058)
                     /locus_tag="PKY68_07210"
     CDS             complement(1099..2058)
                     /locus_tag="PKY68_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOH24857.1"
                     /translation="MKKAILYLTVFMLFAACEKEVRFKGEVTEPLLVLNGFAQPDSLL
                     VIRLTASRFFLEEVDSFVTVKDAHLRLYVDGTYKEDLQSQKNGYYISSYRPKQGEVLS
                     IEAEAPGFESVRAEALLPVVAELLKVDTSITLEGELYDLSYDWDPEVGDYIIDTIGLY
                     TNYQVSMTLSFRDPPTPGDYYRVVVKHFYGKEYYDGYYDFTVENFYTAEMGDLLGPAE
                     TQNEYQIYADELYNGKTVDLKLSFAAHTYRDYQGELPNSTTSIQVQLQNISPSYYLYL
                     KTFSEYSNSIDFFSEPIQVFSNVSGGIGIFGAYSSNVIELKFN"
     gene            complement(2068..>3673)
                     /locus_tag="PKY68_07215"
     CDS             complement(2068..>3673)
                     /locus_tag="PKY68_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH24858.1"
                     /translation="QATKRGMDELDKLSAGYYFYDFNAKINHKFSDRDRLYLSFYSGD
                     DAIYAKVRNKYTESKTYKSTSAIGLDWAWGNLIAALRWNHIINSKMFLNTTASYTRYR
                     FDMSIGLKDENWEYQPPSYLATDIMMGYRSGIEDYTIKADLDYTPNPGHTVKLGANYT
                     YHTFKPGVTVAKFRMDDDSVHQNLLDTVFGDSFVYAHETMLYAEDNIDLGRYVKANLG
                     LHYSTFFVQRQFYHSLQPRLGLRFLFSDNFSFKAGYAMMNQYIHLLSNSDISLPTDLW
                     VPVTKRITPMNSHQVSAGLFYNLRNLVDLSVEGYYKSMHNILEYKDGASFMGSTTGWE
                     DKVSMGRGWSYGLEFLAQKSYGRYTGWIGYTWAKSERLFDRPGQELNNGKVFPAKYDR
                     RHDLSITASYKLNDRIDFAASWVYSSGNCATLALQNYEGSPVPDSYQYYFSSLPYIES
                     RNNYRFESYQRLDIGVNFRKKLKRGTRTWNISAYNAYNRNNPFFVYPSHSERYDPVSR
                     QYVTKRALKQVSIFPLIPSISYSYSF"
BASE COUNT         1009 a          880 c          849 g          935 t
ORIGIN      
        1 gcaaaaactg atcccggcac tgctgcagag caatatcact tcaaactaat attacttcag
       61 ataagaatta cttcagataa gaattacctc agataagaat cgaaatattc gatgaccggt
      121 ggagcggaaa acgggactcg aacccgcgac cctcagcttg ggaagctgat gctctaccaa
      181 ctgagctatt tccgcgttct aaaaaactat gcgaaagtac aagaaaaaat ttttggacgc
      241 aacaaataaa gcgagattac tgcggccatt cttctttgtc gagcaaatag cggtacatag
      301 gcttgatccg ggcccggcgg atttcttcgg ctaccagggc cgccaattgc ttggcgcctc
      361 tttctcttaa atgggtgtcg tcgttcgagc cctgaggcct ggaaggatct tcgcccggtt
      421 cgagaaacat gtaaagcttg cgggcttcct ccgggccgag ttcctgcaat aggtcaaagc
      481 tgagtcgaaa catatcgatc aggatcactt tttcctcggc tgccagatcg cgcactatct
      541 gaggatattc cttcagacgg cggtctacga gctgccccga atcgttgaac gtacacaaca
      601 cgatcgaagt gagcagcacc ggcgtagctt cccgctcgcg tacttcctgc acaaaacggc
      661 gcagattttg ccggtaatcc gccggcgatg catgacgttc gggcttggtc gaagtgtcgt
      721 tgtgtccaaa ctgaataaac accacatcgc ctttgcggag atcttcgaga attttgttcc
      781 aacggccctc ttcgcgaaag gacttgctgc tgcgaccggc catggagcga ttttcgactt
      841 gaacccgttc gtcgaagaag agctcgaggt attgcgccca accccgcatc agggtttgtt
      901 ccacacgata gctctgagcg gtggaatcgc cggccacaaa aagccggatg tcttcttttt
      961 gagtgcagga catgcccagc agagccagga ttgaaccaca caacaatgca atatttttaa
     1021 acgcgggttt tttcatagct tgaatcgttt agtattttat ttcttgtaat cttgctttgt
     1081 aggattcttt gatctccatc aattaaactt cagttcaata acattggaac tgtaagcacc
     1141 aaaaattcca attcctccgc ttacattact gaaaacttgt atgggttcgg aaaaaaagtc
     1201 aatagaatta ctgtattccg aaaaagtctt gagatacaga taataagatg gagatatatt
     1261 ctgcaattga acctgaatac tggtggtcga attgggaagt tccccctgat aatcacggta
     1321 ggtatgtgca gcaaaactca atttcaaatc gaccgtttta ccgttgtaaa gctcatcggc
     1381 gtatatctgg tactcatttt gggtttcggc cggacccaac aagtctccca tttcggccgt
     1441 gtaaaaattt tcaacggtaa aatcataata accatcatag tattctttgc catagaaatg
     1501 ctttaccacc acccggtaat aatcgccggg ggtaggcgga tcccgaaagc tcagagtcat
     1561 gcttacctga taattggtat acaaacctat cgtatcaata atataatctc cgacttccgg
     1621 atcccaatcg tacgaaagat cgtatagttc cccctccaag gttatggagg tatccacttt
     1681 gagcagttct gcaaccacag gcaacagggc ttcggcccgg acagattcaa aaccgggagc
     1741 ttcggcttcg atactcaaca cttcgccctg tttggggcgg taggaactga tatagtaacc
     1801 gtttttctgc gattgcaaat cctctttgta cgttccgtcc acatataaac gaagatgggc
     1861 atccttaacc gtaacgaagg aatcgacttc ttccagaaag aacctgctgg ccgtcagcct
     1921 aatcaccaac agtgaatcgg gttgagcaaa accatttaat accagcagcg gctcggtgac
     1981 ctcaccctta aaccgaacct ctttttcgca agccgcaaag agcataaaga cagtaagata
     2041 taatatcgct tttttcatag ggccgaatca aaaagagtaa gaataactga tagaaggaat
     2101 gagagggaaa atggaaacct gcttgagcgc cctttttgtc acatattgcc ggctaacagg
     2161 atcgtatcgt tccgaatgag acggatatac aaaaaaggga ttgttgcgat tgtaagcatt
     2221 gtaagcgcta atattccagg tacgggtgcc gcgcttaagt tttttgcgga aattcacccc
     2281 gatatccagt cgctgatatg attcgaagcg ataattgttg cgcgactcga tgtatggaag
     2341 actggagaaa taatactgat aactgtcggg caccgggctc ccttcgtaat tttgcaaagc
     2401 caaggtggcg caattgccgc tgctgtatac ccaactggcc gcaaaatcta tgcgatcgtt
     2461 gagtttgtag gaagccgtaa tgctcaggtc gtgacgacga tcgtacttgg ccggaaacac
     2521 ttttccgttg ttgagttcct gaccggggcg gtcaaacaag cgttccgatt tggcccaggt
     2581 ataacctatc caacccgtgt aacgcccata agacttttgc gccaaaaatt ccagtccgta
     2641 ggaccaaccc ctacccatgc tgactttgtc ttcccaaccg gtggttgaac ccataaaaga
     2701 ggcaccgtct ttgtattcca agatattgtg catcgattta tagtaacctt ccaccgataa
     2761 atcaaccaga ttcctgaggt tataaaacaa accggccgac acctgatgcg agttcatggg
     2821 tgtaatgcgc ttggtaaccg gcacccaaag atcggtcggc aggctgatgt cgctgttgga
     2881 caggagatga atgtattggt tcatcatggc gtaaccggcc ttgaacgaga aattgtcgct
     2941 aaacagaaaa cgcaaaccca gccgcggctg cagcgaatga taaaattgcc gctgcacaaa
     3001 aaaggtggaa taatgcaaac ctaaattagc cttgacatag cggcccaaat cgatgttgtc
     3061 ttccgcgtaa agcatggttt cgtgagcgta cacaaagcta tcgccaaaga cagtatctaa
     3121 aagattttga tgaacgctgt cgtcgtccat ccggaacttg gcaaccgtaa caccgggctt
     3181 aaaagtgtgg taagtatagt tggctcccag cttgacggta tgcccgggat tgggggtata
     3241 atccagatcg gctttgatgg tgtaatcctc aatgcccgaa cgataaccca tcataatgtc
     3301 cgtggctaaa tagcttggag gctggtattc ccaattttca tctttcaacc cgatggacat
     3361 atcgaagcgg taacgggtat aggaggccgt ggtattcaaa aacattttac tgttgatgat
     3421 gtgattccag cgcagagccg caatcagatt gccccaggcc cagtccagcc cgatggcgga
     3481 agtcgattta taggtcttgg actcggtata cttatttctt accttggcat aaatggcatc
     3541 gtcgcccgaa taaaaactca gatacaagcg gtctctgtcg gaaaacttat gattgatctt
     3601 ggcgttgaaa tcgtaaaagt aataacccgc cgacagttta tccaattcat ccattccgcg
     3661 cttcgtggcc tga
//
LOCUS       DAOSGL010000230         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_23409_length_3657_cov_3.369517, whole genome shotgun sequence.
ACCESSION   DAOSGL010000230 DAOSGL010000000
VERSION     DAOSGL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23409_length_3657_cov_3.369517"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2953
                     /locus_tag="PKY68_07220"
     CDS             <1..2953
                     /locus_tag="PKY68_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HOH24859.1"
                     /translation="GRGGGYLPTGIAWYRKEFLLPAACEGRRVFIEFDGVMANSEVYI
                     NGKLLGKRPNGYVSFSYDMSEHLRYGENNQLSVRVDNSIQPASRWYTGSGINRHVRMS
                     IKSPVYLPQWGVFVRSSKVSSERAELDVQIDVRNDGTERADVQIVSVLRDASGKEVRR
                     SNSNLKALGVGDSGRVDQTLTVNRPRLWSPEDPALYTLEVSLYKGNELLDTEIVTIGI
                     RSIRFDPAQGFFLNEKNVKMYGVCLHSDGGAMGTAVPLSLWEYRLEKLRDLGVNAIRL
                     AHNPVAPEFLDLCDRMGFLVMNESFDTWQAAKNHAEKGYNLFFKDWWYADTRDQVLRD
                     RNHPSVVIFSVGNEIRDNLNNPEGFAKYKNQHDCVHKYDGSRPVTMALFRPNSSGVYQ
                     NGFAEMMDVVGQNYRVEELVAYHEAHPEKAVIGTENIHDIASWLVLRDKPYMSGQFLW
                     TGIDYLGEAFWPNVMYTISLLDITAEPKPQGLQRKSWWSSEPMVAMVRKSDNNGAGHW
                     VSDWTPIDLDTYDQAEIEVYSNCEEVEILLNGVSQGRKTMDKNAAPAAYRLTFRPGEC
                     KVLGYNQGKKVAETSYHTAGDPDKIVLTTSKQVLNNDWDEVAVVRATVVDEKGIPCVT
                     ANHLIRFEVQGAGRLVATGNANPQNHEMYVVPEKTAYQGTCIAIVRAGSDQGKITIKA
                     EAEGIRGEAQLELSAAAAKVNSLVRPETELQALEQTPLQTLQTSQTPSQTSVQTSSQT
                     GSPNSRQQAKFEKTLLRGDEDGVGILAYGAGDFGFFMPETAGYFKLGLMSEDKSLWLS
                     DARKANIYPTSKGWIMEFQDPFPGTNALRFELRPLQHSNGFVVELKTSQLPGNLSLVW
                     TYGGCSGSKDPAERELFLRPEQCPNNVFSRENNTFTVYYGLSTSLRVMMGVAPMDTET
                     RIGNARSMDNPLMLWNSGKKTDTPVMVARNPLRANSTYYYCIYRQNRKSDYNAFMLPA
                     LFQESDKNLQP"
     gene            2956..>3657
                     /locus_tag="PKY68_07225"
     CDS             2956..>3657
                     /locus_tag="PKY68_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOH24860.1"
                     /translation="MYRIILVSLFGLLISPVVFANYNVRDFGAKGDDKTIDSPAINRA
                     IEAASQAGGGTVFFPAGTYLSYSIRLSNNIHLYFDEGAVLKAARPSAEEGYDPAEPNP
                     HDAYQDFGHSHWQNSLIWGIGLKNIRISGFGTIYGYGLSREESRLTGAANKAIALKEC
                     INVDIQDITMIRCGHFALLATGVENLGIRGVKIDTNRDGLDIDACRNVRISDCTVNAP
                     WDDAIVLKASYALGYY"
BASE COUNT          972 a          909 c          943 g          833 t
ORIGIN      
        1 cggcagggga ggtggttatt tacccaccgg gatagcctgg taccgcaaag aatttctatt
       61 gcccgccgcc tgcgagggac gtcgggtgtt tattgaattc gacggagtta tggccaacag
      121 cgaagtttat atcaacggaa aattattggg aaaacgcccc aacggttatg tgagtttttc
      181 ttacgatatg agcgaacacc tccgttatgg cgaaaacaac caattgagtg taagggtgga
      241 caattccata caaccggctt cgcgctggta taccggttcg ggcatcaacc gtcacgtcag
      301 aatgtctata aagtcgcccg tgtatctgcc gcaatggggt gtgtttgtac gtagttccaa
      361 agtcagctcg gaacgggcag aactcgatgt gcagatcgac gttcggaacg acggaacgga
      421 aagagcagac gtacagatcg tgagcgtact cagagacgct tcgggcaagg aagtccgcag
      481 gagcaacagc aacctgaaag ccctcggagt cggagattcg ggccgtgtgg atcagacctt
      541 gacagtaaat agacccaggc tttggagtcc ggaggatccg gcgctttata ccctcgaagt
      601 gtctttgtat aagggcaatg aattgctcga taccgaaata gtaacgatcg gaatacgcag
      661 tatccgtttc gatccggcac aaggcttctt tctcaacgaa aaaaatgtca aaatgtatgg
      721 ggtttgtctg cattccgacg gaggagccat gggcacggcc gttccgctga gtttatggga
      781 ataccggctc gaaaaactcc gcgatctggg cgtgaatgcc atccgcctgg cacataatcc
      841 tgtggctccg gaatttttgg atttatgcga cagaatgggc ttcctggtga tgaacgaatc
      901 gttcgatacc tggcaggcag ccaagaatca cgccgaaaaa ggctataatc ttttctttaa
      961 agactggtgg tatgccgata cccgcgatca ggtactgcgc gaccgcaacc atccgtcggt
     1021 ggtgattttc agtgtgggca acgaaatccg cgacaacctc aataatcctg aaggttttgc
     1081 caaatacaaa aaccagcacg attgcgtcca caagtacgat ggcagcaggc cggtgactat
     1141 ggcattgttc cgtcccaaca gttcgggtgt gtatcaaaac ggctttgccg aaatgatgga
     1201 tgtggtagga caaaactacc gggtggaaga attggtggcc tatcatgaag cacatcccga
     1261 aaaagccgtg attggaaccg aaaacatcca cgacatagct tcctggttgg tgttgaggga
     1321 taagccctat atgagcgggc aatttttgtg gacgggtatc gattacctgg gcgaagcctt
     1381 ctggccgaac gtgatgtaca ccatttcgct gctcgatatt accgccgaac ccaaaccaca
     1441 ggggctgcaa cgcaaaagtt ggtggagcag cgagcccatg gtagccatgg ttcgcaaatc
     1501 ggacaataac ggggccggcc attgggtctc cgattggaca cccattgatt tggataccta
     1561 cgatcaggcc gaaatagagg tatattctaa ttgcgaagag gtggaaattc tgctcaacgg
     1621 agtctctcag ggcaggaaaa caatggataa aaacgctgcg cctgccgctt accgtttgac
     1681 ttttcgtccc ggagaatgca aagtattggg atataatcag gggaaaaaag tggccgaaac
     1741 ttcttatcac acggccggcg atcccgataa aatcgttctg acgaccagta aacaggttct
     1801 aaacaacgac tgggacgaag tggccgtggt cagggctacc gtagtggacg aaaagggcat
     1861 accctgtgtt acagccaatc acctcatccg cttcgaagtg cagggtgccg gacgtctggt
     1921 ggcaacgggc aatgccaatc cccaaaacca cgagatgtat gtcgttcccg aaaaaacagc
     1981 ttaccaaggc acttgtatcg ccattgtcag agccggcagc gatcaaggta agattacgat
     2041 caaggccgaa gccgaaggca tccggggaga agctcaactg gaactcagtg ctgccgccgc
     2101 aaaagtcaac tcattagtcc ggcctgaaac agaattgcaa gccttagagc aaaccccgtt
     2161 gcaaacccta caaacttcac aaaccccatc acaaacctcg gtgcaaactt cgtcacagac
     2221 cggctctcca aactcccgtc aacaagctaa attcgagaaa acactattga gaggcgacga
     2281 ggacggggtc gggatactgg catacggagc cggcgatttt ggttttttta tgccagaaac
     2341 ggccggttat tttaagctag gcctgatgtc ggaagacaaa tctctctggt tgagcgatgc
     2401 ccgcaaggcg aacatttatc ccacttccaa aggctggatc atggaatttc aagatccttt
     2461 ccccggcacc aatgccctgc gttttgaatt gagacctctg cagcacagca acggctttgt
     2521 tgtagaactc aagacctctc agctacccgg gaacctcagt ttagtctgga cttacggagg
     2581 ttgttccggc tcgaaagatc cggccgaaag agaacttttt cttcggcctg aacaatgtcc
     2641 caacaatgta ttcagtcgtg aaaacaatac cttcaccgtt tattacggct taagcaccag
     2701 tctcagggtg atgatgggcg tggcgcctat ggataccgaa acccgtatcg gaaatgcccg
     2761 atctatggat aatccgctga tgctgtggaa ttcaggtaag aaaaccgata ctcccgttat
     2821 ggtggcccgc aacccgctga gagccaattc tacctattat tattgcattt accggcaaaa
     2881 cagaaaaagc gattacaacg cctttatgtt gccggccctg tttcaagaat cagacaaaaa
     2941 cctgcaacca tgaaaattta tcgaatcatc ttagtctcac tgtttggcct tttgatcagt
     3001 cctgttgttt ttgccaatta caacgtaagg gattttggtg ccaaaggcga cgacaaaacc
     3061 atagattctc cggccatcaa tcgggcaatc gaggctgcct cgcaggcggg agggggtacc
     3121 gtcttttttc ctgccggcac ttatctttca tattctatcc ggctctccaa caatatccac
     3181 ttgtatttcg acgaaggagc agtattgaaa gcagcccgtc cttcggccga agagggttat
     3241 gaccccgcag aacccaatcc tcacgatgct tatcaggatt ttggacacag ccactggcag
     3301 aacagcctga tttggggtat aggattgaag aatatccgta tcagcggctt cggtactatt
     3361 tacggttacg ggttgagccg cgaagaaagc cgtttgaccg gagccgccaa caaggccatc
     3421 gctctcaaag aatgtatcaa tgtagatatt caagacatca ccatgattcg ttgcgggcat
     3481 tttgccctcc tggccaccgg ggtcgaaaac ttaggcatca ggggagtcaa gatcgatacc
     3541 aaccgcgacg gtctcgatat cgatgcctgc cgcaatgtcc gtatcagcga ttgcacggtc
     3601 aatgctccct gggacgatgc catagtgctc aaggccagct atgcgctcgg ttattac
//
LOCUS       DAOSGL010000231         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_23445_length_3652_cov_4.827634, whole genome shotgun sequence.
ACCESSION   DAOSGL010000231 DAOSGL010000000
VERSION     DAOSGL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23445_length_3652_cov_4.827634"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKY68_07230"
     CDS             complement(<1..494)
                     /locus_tag="PKY68_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOH24861.1"
                     /translation="MKAVLFAAGLGTRLYPHTLDKPKALVSIQGRPMLYRVMDKLYEA
                     GIREYVVNVHAFAGQVEKALELYALSKPGISFDISDERDLLLETGGGLKKMAAFLKDG
                     PFLVHNVDVLSDIDLKLFMEQALGLLSVPFQSQTQARPQLRAQTRPQPRVQARFQSQV
                     VPPQS"
     gene            complement(563..3391)
                     /locus_tag="PKY68_07235"
     CDS             complement(563..3391)
                     /locus_tag="PKY68_07235"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH24862.1"
                     /translation="MSLLGLSAAMSAQVNFSLTYTFDLVNKSSGRVDPTPPPEQEGLL
                     FFPFKAVAENSVWGLATDPSSSKNFSFKSWPRAEIDSLPATGTIDLNQYYEFSICVLP
                     SYSLHIDRLSFLVERSGTGIRRCAVRTGRDQFSTNLSFVYDKQDDKIMVLEDHSLELK
                     DMSLTTSGTVYSTCALSVTGPDTLVFRFYGWSAESKLGTFCLDDVCIQGSITSGTEEQ
                     TTADTIIAGGSYSFPARAFDELQLRFGLTTESDSSEVSENFQQSISMQRAGIDAALNL
                     YNMQDSSILEQIQLEIELKDSLGIICNHYFDINYYLPDSVGATSNNMALLRIFAKPQI
                     PEDYLGPYLLGGRFSYVDRLSEQTVVNLLYPMGIYPGASPLCRLNYFLPFKSETGLPP
                     SETVLPPGEGLNTAGHYIPLGLIIENHGPGVAREISLTHPVNNTPGELLLLGWEQNNG
                     ILPIAGDSISIDSLAPGQSRSFIWWFKGNAPALIDSFHFNTLHEDPFKHPDTALLSVS
                     GQLPLYRLVQAYDELDDNQSDFLINSHCDSLHWPDSLILSQGLHFPVYAADSLKFNTS
                     PWDADSLLIFSLFPAQEGWNYFRTAHPGLNGQAKVLSVKRMEDALELPARNVWLSSLP
                     EAEATNPTAVNYLHILDNSPSNEPMSYEVVLAQHHNELIVDSLRIALCRGDVYSFGGQ
                     SITEAGIYYDTIYAESAPDTLQILRLELLARSYVELSDSVCIGESYTGNGFNLPPHQE
                     AGTFIYTDTLQNQWGCDSILHLSLAVCAPPEAPGPIYGDSMVVVAGNYTYTIKPVPNA
                     AFYIWTVLPPYWEKTVQGHSLSLNIPYLGSGTISVRAVNCCGESAGSSLQINGQSGLD
                     GQETTSSFSVFPNPSTSGFVLETSGIEGETTICISSMTGQILHRQIKYLSAEDSRFEF
                     SLDDYVQGMYIISIVNEQVSGVEKLIRE"
BASE COUNT         1055 a          868 c          818 g          911 t
ORIGIN      
        1 gattgaggcg gaacaacttg ggattggaac cgggcttgaa ctcgaggctg aggccgggtt
       61 tgagctcgaa gctgaggccg ggcttgagtc tgggactgaa aaggaaccga gagcaagccc
      121 aacgcctgtt ccataaacaa tttcagatcg atatcactta aaacatcgac gttgtgtacc
      181 aaaaaaggcc cgtcttttaa gaaggccgcc atttttttca ggcctcctcc ggtttcgagc
      241 aacaagtccc tttcgtccga aatatcgaaa gaaataccgg gtttactcag ggcgtaaagc
      301 tccagggctt tttccacctg gccggcaaaa gcatgtacat ttaccacata ttccctgata
      361 ccggcctcgt acagtttgtc cattacccgg taaagcatgg gacgtccctg tatactgacc
      421 agggctttgg gcttatccag ggtgtgagga tataaacggg tacctaaacc cgcagcaaac
      481 aagaccgctt tcatttcaga tgcattgaac gacaaagata gggaaactta gacaagtaag
      541 tttccctatc ccggcgaaat gcttattcac gaatcaattt ttcgacccct gaaacttgct
      601 cattgactat gctgattata tacattccct gaacatagtc gtccagcgag aattcaaaac
      661 gactgtcttc agcactcagg tatttgattt gtctgtgcag gatttgtccc gtcatggacg
      721 aaatacaaat ggtcgtttcc ccttctatgc cggaagtttc cagtacaaag cccgaagtag
      781 agggattagg gaacacactg aaactgctgg tcgtttcctg cccgtcaaga ccggattgtc
      841 cattgatctg caaactgctc ccggccgatt ccccgcaaca atttacagcc ctaaccgaaa
      901 tagtgccgga acccaaataa ggaatattca aactcaggct atgaccctgc actgtttttt
      961 cccaataggg aggcaatacc gtccagatat aaaaagcggc attaggaacg ggtttaatgg
     1021 tataggtata attgccggcc actaccacca tactgtcgcc gtaaatcggg ccgggagctt
     1081 cgggcggagc acaaaccgcc agactcaaat gaaggatact gtcgcagccc cattgattct
     1141 gcaaagtatc tgtataaatg aatgtccctg cttcctgatg cggtggcaga ttgaatccgt
     1201 tccctgtgta ggactcaccg atacagacac tgtccgacag ttcaacataa ctgcgtgcca
     1261 acaactccag tctgagaatc tgcaaggtat cgggcgccga ttcggcataa atagtgtcgt
     1321 aataaatgcc tgcctcggta atcgactgcc ctccaaagct gtaaacatcg ccccgacaaa
     1381 gagcaattct cagactgtcg actatcagtt cattatggtg ttgggcaagt acaacttcgt
     1441 aagacatagg ctcgttcgaa gggctgttat ccaaaatatg caggtaattg acagccgttg
     1501 ggttcgtggc ttcggcttcc gggagcgaac tcagccagac gttccttgcc ggcaattcca
     1561 gggcatcttc catgcgtttc acagacaaaa ccttagcttg cccattcaaa cccggatgag
     1621 ccgtcctgaa ataattccaa ccttcctgag ccggaaacaa actgaaaatc aaaaggctgt
     1681 ccgcatccca ggggctggta ttaaacttca aactatccgc tgcataaaca ggaaagtgca
     1741 aaccctgaga aaggattaga gaatccggcc aatgcaggct atcacaatgg ctattgatca
     1801 aaaagtcgct ttgattatcg tccagttcat cgtaagcctg caccagcctg tatagaggaa
     1861 gttgtccgga aacggataac aatgcagtat cgggatgttt aaaaggatcc tcgtgcaaag
     1921 tattgaaatg aaaagaatcg atcaatgccg gtgcattgcc tttgaaccac catataaagc
     1981 tgcgactttg tccgggagcc agactgtcga tagaaatact atcaccggcg attggcaata
     2041 tgccgttgtt ttgctcccag cccagaagca gcaactcgcc cggggtattg ttgacggggt
     2101 gagtcaaact gatttccctg gccacacccg gaccgtggtt ttcaatgatc aaacccaaag
     2161 gaatataatg cccggccgtg ttcaaacctt ctccgggcgg caagaccgtt tcggagggtg
     2221 gcaaacctgt ttcagactta aaaggcaaaa aataattaag tctgcacaaa ggcgaagctc
     2281 cgggataaat acccatggga tacagcagat ttacaactgt ctgttcactg agcctgtcga
     2341 catatgaaaa gcgtcctccc agcaaatagg gcccaaggta atcttccggt atttgaggtt
     2401 ttgcgaaaat ccggagtaaa gccatattat tggaggtagc tcccacgctg tcgggcagat
     2461 aataattgat atcaaaataa tggttgcaga taatacccag gctgtccttg agttctattt
     2521 ccagttgtat ttgctccaga atggaagaat cctgcatgtt atacaggttt agagctgcgt
     2581 caataccggc cctttgcata cttatgcttt gttgaaaatt ttcggaaact tcgctgctgt
     2641 ccgattccgt tgtcagacca aaacggagtt gaagttcatc aaaggcccgg gcgggaaaag
     2701 aataactgcc tccggcaata atggtatcgg cagtggtctg ctcctccgtg ccgcttgtga
     2761 tacttccctg aatacagaca tcgtcgagac aaaacgtacc cagtttactt tccgcactcc
     2821 atccgtaaaa acgaaaaacc agcgtatccg gccctgtaac agacaaagca caggtgctat
     2881 agacagtacc tgaagtcgtt aagctcatat ctttcaattc aagactatga tcttccagaa
     2941 ccatgatttt atcgtcttgt ttatcgtaaa caaaagacaa atttgtcgag aactgatccc
     3001 tgccggttct gacggcacaa cgccttatac cggttcccga gcgctcaacc aggaaagaga
     3061 gcctatcgat gtgcaaagag tacgacggaa gcacacagat actgaactca tagtattgat
     3121 taagatcgat cgttcccgta gcgggcaggc tgtcgatctc ggcccttggc caggatttaa
     3181 acgaaaaatt cttgctcgaa ctgggatctg ttgccagacc ccatacggaa ttttcggcca
     3241 cagctttgaa cggaaaaaac agcaagccct cttgctcagg aggaggagtc ggatctaccc
     3301 ggccgctgct tttgtttact aaatcaaaag tataagtaag tgaaaaattt acctgagcgc
     3361 tcatggctgc actcagaccc aaaaggctca aacataaaac aatatgtctg aagaatttac
     3421 gattaataaa tagcctttta tcagaataat taatattaat gtttttcatt ttggaagtgt
     3481 attaattggt ttgaatttat cttttgatag cgcaaactta atattaatac aattaccaaa
     3541 caaacatttt aaaaaggatt tgtgttaaat aaaatcgtaa aagacaggcg ggtttcttaa
     3601 aaagagcgca attctttgac cgtcaacata tgggcaaaac agctatgaat tt
//
LOCUS       DAOSGL010000232         3621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_23734_length_3621_cov_3.971957, whole genome shotgun sequence.
ACCESSION   DAOSGL010000232 DAOSGL010000000
VERSION     DAOSGL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23734_length_3621_cov_3.971957"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="PKY68_07240"
     CDS             complement(<1..1603)
                     /locus_tag="PKY68_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOH24863.1"
                     /translation="MIYPQNFEIKTGFDQIRLLLQEQCSSTLGKEKVLEMCFSDDYKD
                     ILLALELSAEFLAVLHGEQTFPEILFFDLRAVLKRLQVEGSYADQKELFDLKRSLDSY
                     HKVLLFLNHKEQATLLYPRLKELAAGQQTFPRIIEQIDSILDKSGEIQDHASPELAQI
                     RKKISITTAGISRKLHILIKKAQEEGIVEKGSSPSVRDGRLVIPVPPAHKRKISGIVH
                     DESASGKTVYIEPAELVEANNQVRELQSEEKREILRILIQFADYIRPLNEELLAAFDY
                     MAQIDFIRAKALLAQKINALKPQLSDQSVVNWKGAIHPLLFLSLQKQGKEIVPLDIQL
                     NEQQRILVISGPNAGGKSVCLKTTGLLQYMLQCGLLIPVQENSVCGIFSKLFIDIGDE
                     QSIENDLSTYSSHLNNIKFFLKNSDKKTLLLIDEFGSGTEPKIGGAIAETCLDLFKQK
                     GLFGVITTHYDNLKHYAEDNEGVVNAAMLYDRHLMQPLFRLEIGRPGSSFAVEIARTI
                     GLPREVIDKATEIVGSDHIDMDKYLQ"
     gene            complement(1604..3265)
                     /locus_tag="PKY68_07245"
     CDS             complement(1604..3265)
                     /locus_tag="PKY68_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HOH24864.1"
                     /translation="MTWLTELIQNDSVARTILILAVVISSGIYLGKIKIFGISLGITW
                     VLFVGLILGHFGFSIQPTVLHFIKEFGLILFVYSIGMQVGPGFFSSFKQGGIRLNMLA
                     VGVVLLGVLTVLGFFWLTDYPMTTLVGIMSGAVTNTPGLGAAQQAYLEIYQSNAPDIA
                     LGYAVAYPLGVVGVIGATILIRILYKIKFQDESRRLQEEYERNHNSFVLASVELMNPA
                     LFGKTVEEIHRLSLSQFVISRVLKSDQRMELCNNTTKLEEKDKILIATDSGDLQAVIA
                     FFGKRIEMDWDKIPSDLVSRRILLTKTDVNGKTLGSLHLRKLYGVNVTRVNRSGMDLL
                     ASPSLVLQMGDRLMVVGSLESIQKIESLLGNSLKRLREPNLIPIFLGIFLGVLLGSVP
                     IRFPGIPQAVKLGLAGGPLIVAILISRFGPKYGLLTYTTMSANLMLRELGITLFLCSV
                     GIGAGDLFVETVFNTQGLSWLGIGLVITVLPPLIVGILARSVFKLNYLDIMGLLSGSM
                     TDPPALAYANSSSGSDQPVVAYSTVYPLTMFLRVLCAQILILIFA"
BASE COUNT          987 a          788 c          829 g         1017 t
ORIGIN      
        1 cctgcaagta tttgtccatg tcgatatggt ctgagcctac tatttcggtg gctttgtcga
       61 tcacttcccg gggcaggcca atggtacggg caatttcgac ggcaaaagaa ctgcccggcc
      121 ggcctatttc caatcggaac aggggttgca tcagatgccg gtcgtagagc atggcagcgt
      181 ttacaacgcc ttcgttgtct tcggcataat gcttgagatt gtcgtaatgc gtggtgataa
      241 cgccaaacag ccccttttgc ttgaagagat caagacaggt ttcggctatg gcgccgccaa
      301 ttttgggttc ggtcccactg ccgaattcgt ctatcagcaa taaggtcttt ttgtctgaat
      361 ttttaagaaa aaacttgatg ttgttcagat gtgagctata ggtgctcaag tcgttttcta
      421 tggattgttc gtcgcctatg tcgataaaaa gtttggaaaa aattccgcaa accgaatttt
      481 cctgcaccgg gatcaataag ccgcattgaa gcatatattg cagaagaccc gttgttttga
      541 ggcaaaccga ctttcctccg gcgttgggcc ccgaaatcac taatattctc tgctgttcgt
      601 tcaactgaat atcgaggggc acgatttctt tgccttgttt ttgcagggaa agaaagagta
      661 ggggatgtat ggctcctttc cagtttacga cactttggtc cgacagttga ggcttaaggg
      721 cgtttatttt ttgagccagg agtgcttttg ccctgataaa gtctatttgg gccatataat
      781 cgaaagcagc cagcaattct tcgttcagag gcctgatgta atcggcaaat tgtatcagaa
      841 tgcggagaat ctctcttttt tcttcgctct gaagttccct gacctggttg ttggcttcga
      901 ccaattcggc cggttctata taaacagttt taccgctggc cgactcatcg tgtacaatac
      961 cgctgatctt gcgtttatgg gccggaggaa cgggaatcac caggcggccg tcgcgcacac
     1021 tggggctgct gcctttttcg acgatgcctt cttcctgagc ttttttgatc aatatgtgca
     1081 gtttgcgcga gatgcccgct gtagtaatgg agattttttt tcggatttgt gccagttccg
     1141 gggaggcatg atcctgtatt tcgccggatt tatcgaggat gctgtctatc tgttctatta
     1201 ttcgtgggaa agtttgttgt ccggcagcaa gctcttttag cctgggatag agaagtgtag
     1261 cttgttcttt gtggtttaaa aaaagcaata ccttatgata actgtccaat gagcgtttta
     1321 aatcgaacag ttctttttgg tcggcataac ttccttcaac ctgaaggcgt ttcagcacag
     1381 ctcttagatc gaaaaataaa atctcgggaa aagtctgttc accatgtaat acagccaaaa
     1441 attccgcgct gagttccaag gcaaggagta tgtctttgta atcgtcggaa aaacacattt
     1501 ccaagacttt ttccttacct aaagtactgc tgcattgctc ttgtagcaaa aggcggattt
     1561 ggtcaaagcc tgtttttatt tcaaaattct gcggatagat catttaagca aagatcaaaa
     1621 tcagtatctg cgcgcaaagg acacgtaaaa acatggtcaa aggataaacg gtggagtaag
     1681 caactacagg ttggtcactg ccggaactgc tgttggcgta ggccagggcg ggaggatcgg
     1741 tcatacttcc cgatagtaat cccattatat ccaggtaatt caatttgaaa acactgcggg
     1801 ctaaaatgcc aacgataagc ggtggtaaaa cggtaatcac caaacctatg ccaagccaac
     1861 tcaatccctg ggtattgaaa acggtttcga caaagagatc gccggctcct atgcctacgc
     1921 tgcacaaaaa caaggtaatt cccaactccc tcagcattag gttagcactc atggtggtgt
     1981 aggtgagtaa accgtatttc gggccaaaac ggcttatcaa aatggcaaca atgagcgggc
     2041 ctcctgccag tcccagtttt acagcctggg gtatacccgg aaagcggata ggtacacttc
     2101 cgagcaaaac tcccaaaaaa atacccagga aaatgggaat cagattaggt tctctaagtc
     2161 gcttaagaga gttgcccagg aggctttcaa ttttttgtat agattccaaa ctgcccacca
     2221 ccatcagacg gtcgcccatt tgcagtacga gcgaggggga ggccagcaaa tccatacccg
     2281 atcggttcac tctggtcaca ttgactccgt agagttttct taagtgcagt gatcccagag
     2341 tttttccgtt gacgtcggtt ttggtgagca ggatacgtct cgaaaccaaa tctgatggaa
     2401 ttttgtccca gtccatctcg atgcgttttc caaaaaatgc aataactgcc tgaagatctc
     2461 ccgaatcggt agctattaga attttatcct tttcttccaa tttggtggta ttgttacaca
     2521 attccatgcg ttgatcgctt ttgagaaccc gagaaatcac aaattgagaa agcgacaggc
     2581 ggtgaatttc ttcaacggtt tttccgaaca gagcgggatt cataagttct acgctggcca
     2641 atacgaaaga attgtggttg cgttcgtatt cttcttgaag acggcggctt tcgtcctgaa
     2701 attttatttt gtacaggatg cgtattaaga tggttgcgcc aatgacaccc accacgccca
     2761 gtgggtaggc aacagcgtaa cccagcgcaa tatcgggcgc attgctttgg tatatttcca
     2821 ggtaagcttg ttgtgcggca cccagaccgg gtgtgttggt aacggcaccg gacataattc
     2881 caaccaatgt ggtcatagga taatcggtta gccagaaaaa gcccagaacc gttaaaactc
     2941 ccaatagaac tacccccaca gccaacatat tcaagcggat tcctccttgc ttaaaagaag
     3001 aaaagaagcc ggggcctacc tgcatgccaa tggagtacac aaagagtatc agaccgaatt
     3061 cttttataaa atgtaacacc gtgggttgga tactgaatcc gaaatgtccc agtatcagac
     3121 caacgaacaa aacccaggtg attcccaggg agatgccgaa aatcttgatt ttgcctaagt
     3181 aaataccact cgaaattact acggccagaa ttaaaattgt acgtgcaacg gaatcgttct
     3241 gaataagttc agttagccaa gtcataatcc tttatataat tggtaattat ctatttatca
     3301 aggttttttt tgatctgcga aggtataaaa aataaacgaa tataaacggc tttcttcgaa
     3361 aaagtatgta agtaagagat ggtcaagtat tgtttagaat ttttcacatg tttggagaag
     3421 aatattttat acctttgggg cgttctttta ttaaccatca catatcataa taacgacaac
     3481 atgaaaaaaa ctgcattatt atttttggct cttttaccca tcatcactgt tttcgcgcaa
     3541 gaagagccca gtgaaacaga taaaattaaa gactcaaggt gtagatgtcg tcggcaacac
     3601 cttctgcacc ggaattcagg t
//
LOCUS       DAOSGL010000233         3598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_23954_length_3598_cov_3.793113, whole genome shotgun sequence.
ACCESSION   DAOSGL010000233 DAOSGL010000000
VERSION     DAOSGL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23954_length_3598_cov_3.793113"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PKY68_07250"
     CDS             complement(<1..1016)
                     /locus_tag="PKY68_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOH24865.1"
                     /translation="MKSFSHILPILLMLSLSSQVCSAPKDSKQPPRLVLGIHLDQLQE
                     EYLKWFMEGFGEEGFKLLLERGKVYNNCNYGLNCPDAANTNASLLSGCAPMHHGIIAR
                     EWYDRQLERPVSCVYDTDYLGNYTTSTFSPLSLRSNTLGDELKKACKGRSKVFSIGLE
                     AEAAIVAGGRSADAALWMDDTSGKWCSSTYYNYMPSWLQKINDYGLFETNNGKNGWRP
                     KFPLSRYVYMPHQKNPGFFSYALSFINGTGYRDFKQTPMANTELNQLALELIDQEKLG
                     EDEYPDLLILHFNTGSQLSGNDSRAALEMQDLYFRLDQDLGWLLKEIDRKIGLDNVLV
                     YLCGT"
     gene            complement(1013..2260)
                     /locus_tag="PKY68_07255"
     CDS             complement(1013..2260)
                     /locus_tag="PKY68_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOH24866.1"
                     /translation="MLFFVGISPGVKAQTVPALSGQAEIYLLTCSPGQEVWAHYGHTG
                     IRVLDPPTRRDVVFNYGIFDFNSDNFLWNFVRGEIDYILGISSFEGFMHSYRNENRRV
                     ISQKLNLSQEEKARLWEALVINSLPENRTYRYNFYYDNCSTRARKIIERCLDGAVDYH
                     YEAAYGSMREVIQHYTVKHPWTWFGISLALSSLADAPAPALDLLFAPELMMEAFEKAT
                     ITAPATNVKDSLQAERSLISEQSILNEIKTVPAEQKNTLPGPVTFSWILFMLVLTLGL
                     RELKTARRYPLVNALIFGLAGIAGLIIAFLALISVHPVTKTNYLLLWLHPLHLLFALL
                     IAFRPFRRSIFYNGYLKINTPVLVFALAGFGFLPQYVHPAMIPLLLSLLSRSLLAWIN
                     PLPKSIRNKKVPGQGSGKSGKNL"
     gene            2526..2909
                     /locus_tag="PKY68_07260"
     CDS             2526..2909
                     /locus_tag="PKY68_07260"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HOH24867.1"
                     /translation="MKIDYSRLGEWVQNNTLLMVFLLGFVAILLFVFLIVRYKNNKNR
                     LLLAQEYLAAGKEIPRYLLRENSLYARAVKNLFLGIGLVFFLGFLTETWGWASAGLLF
                     IALGVGQMIVFKKSSKDFTGSDLDR"
     gene            complement(2940..>3598)
                     /locus_tag="PKY68_07265"
     CDS             complement(2940..>3598)
                     /locus_tag="PKY68_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOH24868.1"
                     /translation="AYCRGGLLIIEGRKEKVPNPRYNPQARDWRTSRQQADYTSSSIT
                     TRNKRSFLYGRFEVRARIPLAGGSWPAIWTLGNTMEWPSCGEIDIMEYYRIKGEPHIL
                     ANAAWGGEQRYKAIWDSSVYPLSHFTNADPDWGSKFHIWRMDWDEQAIKLYLDDELLN
                     EIPLDRTSNGSIGNYSNPFRQEHYLLLNLAIGSNGGKPDESAFPLLYEIDYVRVYQKQ
                     "
BASE COUNT          897 a          819 c          919 g          963 t
ORIGIN      
        1 gtaccgcaga gataaaccag gacattgtcg agtcctattt tccggtcgat ttctttgagt
       61 aaccagccca gatcctgatc caatctgaaa tacagatcct gcatttcaag ggcggcccgg
      121 ctgtcgttgc cgctcagttg gctgccggtg ttgaaatgca agatcaacag gtccgggtat
      181 tcgtcttctc ccagcttttc ttgatcgata agttccagag ccagctgatt gagttcggta
      241 ttggccatgg gggtttgttt gaagtccctg taacccgtac cgttgataaa agagagagcg
      301 taactgaaaa agcccggatt tttttggtga ggcatataca catagcggct cagaggaaat
      361 ttcggcctcc agccgttttt gccgttgttt gtttcgaaca gtccgtaatc gttgattttt
      421 tgaagccacg acggcatgta attgtaatag gtgctactgc accatttgcc actggtatcg
      481 tccatccaga gggctgcatc ggcgcttcgc cctccggcaa caatggcggc ctcggcttcc
      541 agccctatcg aaaagacttt ggaacgtcct ttgcaggctt ttttgagttc atcgcccaag
      601 gtattgcttc tcaaactcag gggcgaaaag gttgaagtgg tgtaatttcc caggtaatct
      661 gtgtcgtaga cgcaggacac gggcctttcg agttggcggt cgtaccattc acgggcaatg
      721 atgccgtgat gcatgggggc gcagccgctc aacaggctgg cgttggtatt agccgcatcg
      781 ggacagttta aaccatagtt gcaattattg tatactttgc cccgttccag cagcaacttg
      841 aagccttcct cgccaaatcc ttccataaac catttcaggt attcctcctg taactgatcc
      901 aggtgtatgc ccaataccag gcggggcggt tgtttgctgt ccttaggagc ggaacagacc
      961 tgagaactca ggctcaacat caacaaaata gggagtatat gtgaaaatga cttcatagat
     1021 ttttaccgga tttgcctgag ccttgcccgg gcactttttt gttacgaatg ctctttggaa
     1081 gaggattgat ccaagccaac aggctacgcg acaataaact cagcaaaagc gggatcatgg
     1141 ccggatgtac gtattgcggc aaaaagccga aacctgccag tgcaaaaact aaaacgggag
     1201 tatttatttt taagtagcca ttgtaaaaaa tgcttcgcct gaaaggcctg aacgcgatca
     1261 acaaagcaaa aagcaaatgc agggggtgca accaaagcag gaggtaattg gttttggtaa
     1321 cgggatgtac cgaaattaag gccaaaaaag caataatcaa gcccgcaatc cctgccagtc
     1381 caaaaataag ggcgtttacc agaggatacc ggcgggctgt tttgagttcc cgcagcccca
     1441 gggtcagaac cagcataaac aaaatccaac tgaatgttac aggtccgggc agggtatttt
     1501 tttgttcggc cggcactgtt ttgatttcgt tcaatatgct ttgttcgctt atcagagaac
     1561 gctccgcttg caggctgtct ttaacgttcg ttgcaggagc tgtgattgtg gccttttcaa
     1621 aagcttccat catcaattcg ggagcgaaaa gcagatcgag ggccggagcc ggagcatcgg
     1681 ccagactgct cagtgcaagg gaaataccga accaggtcca gggatgtttt acggtgtaat
     1741 gttgaattac ttcacgcatg ctgccgtagg cggcctcgta gtgataatcg acggctccgt
     1801 ccaggcatcg ttcaattatt ttgcgggccc gggtggagca attgtcgtag taaaaattgt
     1861 agcgataagt ccggttttcg ggtaggctat tgataaccag agcctcccat agtcttgctt
     1921 tttcttcttg cgagaggttc agcttttgag aaatcactcg gcgattttcg ttgcggtagc
     1981 tgtgcataaa accttcgaaa ctgctgattc ccaaaatata gtcgatttcc ccccgcacaa
     2041 aattccacag gaagttgtcg gagttgaagt caaaaatacc gtaattaaag accacatcgc
     2101 ggcgggtcgg cggatccagc acccggatac cggtatgtcc gtaatgcgcc cagacttcct
     2161 gcccgggaga gcaagtgagc aggtaaatct ctgcctgtcc gctgagtgca ggaactgttt
     2221 gtgctttgac accggggctg atacccacaa agaacaacat tcccattacg aataaaatga
     2281 gggatcgttg tgctttgttg atcaaagaaa aaatcgattt acggcttgct gacatcggtc
     2341 tcttaaaaat caggcaaaaa taggcaattt tagcgagatt gtttgtatga tatggaactt
     2401 gttatgcctt aaaaaaagca gtttaggctt tgatattccg ataaagttac gaactttgcg
     2461 agtctaatac gggtttcggc gaagatacca acgaaaccga taaacagtta taatgccggc
     2521 aaaatatgaa aattgattac agccgattgg gagaatgggt acagaacaac actctgctta
     2581 tggtattctt gctgggtttt gttgccatac tgctctttgt ttttctgatt gttcgttaca
     2641 aaaacaacaa aaaccgtttg ctgttggcgc aagaatacct ggcagcaggc aaggaaattc
     2701 ctcgttattt gctgagagaa aacagcctgt acgccagggc tgttaagaat ctttttttgg
     2761 gtatcggcct ggtctttttt ctgggctttc tcaccgagac ctggggctgg gcttcggccg
     2821 gcttgctgtt tattgccctg ggagtggggc aaatgatcgt ctttaaaaaa tccagcaaag
     2881 attttaccgg cagcgattta gaccgctgat ttcggcagcc tgattctttt tcccatttct
     2941 tactgttttt ggtaaactct gacgtaatcg atctcgtaga gcaatggaaa ggcagattcg
     3001 tcgggttttc cgccgttgct gcctatggcc aggtttagca gcaggtaatg ttcttgccgg
     3061 aaaggattgc tgtaattacc tatgctgccg ttgcttgtac ggtctaaggg aatttcgttg
     3121 agtaattcgt cgtccagata gagcttgatg gcttgttcgt cccaatccat acgccagata
     3181 tgaaatttgg agccccagtc gggatcggcg ttggtgaaat gactcaaagg ataaacggag
     3241 gaatcccaaa tagccttgta gcgctgttct cctccccagg ctgcgttggc cagaatatgg
     3301 ggttctcctt tgatgcgata atactccatg atgtctattt cgccgcacga aggccattcc
     3361 atggtattac ccagggtcca gatggcaggc caggatccgc cggccagcgg aatccgggct
     3421 cgaacttcga agcgtccgta aagaaaagac ctcttgttcc gggtagtgat agaggaagaa
     3481 gtgtaatcgg cttgctgtct ggaagttcgc caatctcgcg cctggggatt atagcgggga
     3541 ttgggaactt tctccttgcg tccttcaata atcaggaggc ctcccctgca ataggcgt
//
LOCUS       DAOSGL010000234         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24096_length_3584_cov_4.026636, whole genome shotgun sequence.
ACCESSION   DAOSGL010000234 DAOSGL010000000
VERSION     DAOSGL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24096_length_3584_cov_4.026636"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKY68_07270"
     CDS             complement(<1..543)
                     /locus_tag="PKY68_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOH24869.1"
                     /translation="MMSFVVLISLIFTATAKTNPNPVPEQVMEEVYHKVKTPHKYGLV
                     LAPGDNFHKMDCPTVYRKGKKWYMTYLVYDGKGGRDGRGYETWLAESNDLLNWKTLGR
                     ILSFQENTWDSNQRGGYMALQDMEWGGSYKIGKHRGKNWITYIGGNSPGYELGTLQIG
                     LAYTEGDISTAHEWKTFPQPL"
     gene            complement(1161..1586)
                     /gene="rplQ"
                     /locus_tag="PKY68_07275"
                     /pseudo
     CDS             complement(1161..1586)
                     /gene="rplQ"
                     /locus_tag="PKY68_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1591..2583)
                     /locus_tag="PKY68_07280"
     CDS             complement(1591..2583)
                     /locus_tag="PKY68_07280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOH24870.1"
                     /translation="MAILAFQKPEKVIMLEADEQFGKFEFRPLEPGFGITIGNSLRRI
                     LLSSLEGYAINSVRIDGVDHEFASIPGVLDDMTNIILNLKQIRFKQIIEDTDFEKVSL
                     TVSGLQELKAGDLGKAFTAFEVLNPDLKICSMEKSVSFNMELTVNKGRGYVPAEEQEL
                     GEVDVNVIAIDSIYTPIKNVKYSVENYRVEQKTDYEKLVLEIATDGSIHPKDALKEAA
                     KILIYHFMLFSEEKITLDVSESDNGEEFDEEVLRMRQLLKTKLVDLDLSVRALNCLKA
                     ANVETLGHLVTFNKTDLLKFRNFGKKSLTELEDLLSKMDLSFAMDISKYKLEKE"
     gene            complement(2596..3201)
                     /gene="rpsD"
                     /locus_tag="PKY68_07285"
     CDS             complement(2596..3201)
                     /gene="rpsD"
                     /locus_tag="PKY68_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOH24871.1"
                     /translation="MARYIGPKSKIARKFGEPIFGPDKVLSKKNYPPGMHGSNRRRKT
                     SEYGIQLREKQKTKYTYGVLERQFRKTFERAARSKGITGEVLLQLLESRLDNLVYRLG
                     LAPTRDAARQLVVHRHIVVDGKVVNIPSAIIKPGQVVGVRERSKALEVIDNALSGFNH
                     CKYPWLEFDAASKSGKYLHVPERADIPENIKEQLIVELYSK"
     gene            complement(3222..>3584)
                     /gene="rpsK"
                     /locus_tag="PKY68_07290"
     CDS             complement(3222..>3584)
                     /gene="rpsK"
                     /locus_tag="PKY68_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOH24872.1"
                     /translation="KRVVKVDAVGQAHIHSSFNNIIVSLANGNGQIISWSSAGKMGFR
                     GSKKNTPYAAQMAAQDCAKVAYDLGLRKVKAYVKGPGNGRESAVRTIHGAGIEVTEII
                     DVTPLPHNGCRPPKKRRV"
BASE COUNT          868 a          819 c          759 g         1138 t
ORIGIN      
        1 caaaggctgt ggaaaagttt tccattcgtg ggcggtactt atatctccct cggtataagc
       61 caatccaatt tgcaaggtgc ccagctcgta accgggagaa ttgcctccga tgtaagtgat
      121 ccagtttttc ccccggtgtt ttcctatttt ataggagcct ccccattcca tgtcctgcaa
      181 agccatgtag ccaccgcgtt gattactgtc ccaagtgttc tcctgaaaag agaggatgcg
      241 tcccagggtt ttccagttca ataaatcgtt gctttcggcc aaccaggttt cgtagccgcg
      301 cccgtctctc ccgccctttc cgtcgtaaac cagataggtc atataccatt tcttaccctt
      361 acgatacact gtaggacaat ccattttatg gaaattgtcg ccgggagcca gtaccaaacc
      421 gtacttatga ggggttttta ctttatgata tacctcttcc atcacctgtt caggcacagg
      481 attgggatta gtctttgcag ttgccgtgaa tataagagaa ataagcacga caaaactcat
      541 catccttctc ataataaaat tataatcgct ttagaaatga acaaacttaa cactttagga
      601 caaatgcgcc acataaatca atctaagata tgcaaataaa gataaactac tgaaggtgta
      661 aaagttatga ttgaaaaact caaaaaaaag ccgcttcaaa attgaaacgg cttttggtat
      721 tgactcaaac tgttatggtt ggagttaaat tattcctgtt tggactcgtc ttcctgtgct
      781 gcttgttcct cagttttgcc ttctgtaggt tcagcgggag cttcggctgt tttggctttc
      841 gaatcagcag gagattcgac ggcttcagct tttggttcaa cggcatcgga ttcagtagct
      901 ttgactgcct tagcctttgg ttctgccacc ttggattcag tagtttcagt agattcggta
      961 gcttcttcag cttctggctc ggtagttttg gactcagcag cctcaacggt ttcagcttct
     1021 ggttcggcag tcttagattc aacagcctca gctgctttag cctttgattc tacaacatca
     1081 gattctgcgg ctttagcctt tggttcagca accttggatt cgatcttttc tgttttcact
     1141 tcggtggccc ctgctttttc ttcactcttc ttttttgaag agcggcgggt acgcgctttt
     1201 ctgtccactt tggttttgag catgctctcg ttgaagtcaa ccaattcgat gaaacacatc
     1261 tgagcattgt cgcccaggcg atagccggtt ttgataatgc gtgtgtagcc gccgggtctg
     1321 tcggccactt tctgagcaac tacctgaaac aattcagtga cggctctctt atcttgcaaa
     1381 aacttaaaag ccaaccttct ggaagccgtg gtatcttctt tggagagggt tattataggt
     1441 tctatttctc ttctcaaagc tttcgctttg gctgtggttg tgaaaatccg tttgtgcata
     1501 atcaaagatg ttgccatatt agacaacatc gatttgcgat gcgctgtctt acgactcagg
     1561 tgatttaatt tcttattgtg tctcattatt ttactctttt tctaatttat acttggaaat
     1621 gtccatggca aaagacaaat ccatctttga caacaagtct tccagttctg tcaaagattt
     1681 cttgccgaaa ttacggaatt tcagtaagtc ggttttgttg aaagtaacca gatgccccag
     1741 tgtttcgacg ttggctgcct tcaggcaatt gagtgcacgt accgacagat ccaaatctac
     1801 cagtttagtc ttgagcagct gacgcatacg taatacttcc tcgtcaaatt cttctccgtt
     1861 atcggattca ctcacgtcta aggtgatttt ttcttccgaa aagagcataa aatgatagat
     1921 caatattttg gcggcttcct tcaaagcgtc tttgggatga atggaaccat cggtggcgat
     1981 ttccagcacc agtttctcgt agtcggtttt ttgttcaaca cgatagtttt cgacggaata
     2041 ttttacgttt ttgatgggtg tgtaaatgga gtcgatggca atgacattca catccacttc
     2101 gcccaattcc tgttcttctg ccggaacgta accgcgtccc ttgtttacgg taagctccat
     2161 attgaagctg acgctttttt ccatggaaca gattttcaaa tccggattga gaacttcgaa
     2221 agcggtaaat gctttaccca aatctccggc tttaagttcc tgcaaaccac tgaccgtcaa
     2281 gctgactttt tcaaagtcag tatcttctat gatttgttta aaacgtattt gcttgaggtt
     2341 cagaataatg ttggtcatat catccaaaac acccggtata cttgcaaatt cgtgatccac
     2401 accgtctata cgcaccgaat tgatggcata accttctaga gatgaaagca atatccgcct
     2461 cagggaattg ccgatcgtaa tcccgaaacc gggctcaaga ggacgaaatt cgaattttcc
     2521 aaattgttcg tccgcttcca acataatcac cttttcaggt ttttgaaatg ctaatatagc
     2581 cataattacg gtgttttatt tagagtacaa ttcaacaatg agttgttctt tgatgttttc
     2641 gggtatatct gctctttcgg gaacatgcag atattttccg gatttagaag cagcatcaaa
     2701 ttccaaccag ggatatttgc agtggttaaa accggaaagg gcattgtcaa ttacttccag
     2761 ggctttggag cgttcgcgta caccaaccac ctggccgggt tttataatag ccgaaggtat
     2821 gtttaccacc ttaccgtcta cgacaatgtg acgatgtaca accaactgac gggcagcgtc
     2881 gcgagtggga gccagtccca gacggtacac caggttatcg agcctcgatt ccagcaactg
     2941 gagcaacact tcgccggtga tacctttaga gcgtgcagcc ctctcgaagg tcttgcgaaa
     3001 ttgtctttcc aatacaccgt aagtgtattt tgttttttgt ttttcacgta gctgtatccc
     3061 gtattctgag gttttcctgc gacgattgct gccgtgcata cccggagggt aatttttttt
     3121 gcttaatacc ttgtcggggc caaaaatggg ttccccaaat ttgcgtgcaa tttttgattt
     3181 cggaccaata tatctcgcca ttgtttaagt cattaataga tttatactct tcttttcttc
     3241 ggaggacgac aaccattgtg gggcaatggt gttacatcga ttatctctgt aacttcgatg
     3301 cctgctccgt gaatggtacg tacggcagac tctcttccgt ttccgggtcc tttgacatat
     3361 gccttgacct ttctcaagcc aagatcatat gcgactttgg cacaatcctg agcagccatt
     3421 tgggctgcgt agggggtatt tttcttcgaa cctctgaatc ccatcttgcc ggcagaagac
     3481 caggatatga tctgtccgtt gccattggcc agggatacaa tgatgttgtt gaaagaagaa
     3541 tggatgtgcg cttgtcctac agcgtcaact ttgacgactc tttt
//
LOCUS       DAOSGL010000235         3581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24128_length_3581_cov_4.745037, whole genome shotgun sequence.
ACCESSION   DAOSGL010000235 DAOSGL010000000
VERSION     DAOSGL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24128_length_3581_cov_4.745037"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="PKY68_07295"
     CDS             complement(<1..115)
                     /locus_tag="PKY68_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH24873.1"
                     /translation="MSETVISVRNLTKSFGNFIAVNNISFDVYKGEIFGFLG"
     gene            complement(112..1011)
                     /locus_tag="PKY68_07300"
     CDS             complement(112..1011)
                     /locus_tag="PKY68_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH24874.1"
                     /translation="MDVIDVSGLSKSFGKVKALDSVSFSVKRGELFGLIGPDGAGKTT
                     LFRLLTTLLNPDTGTASIDGLDVIKDYLKIRSRVGYTPGRFSLYPDLTVEENLIFFAS
                     LFGVKVEDSYDLIAPIYKQIEPFRTRQAGKLSGGMKQKLALSCALIHHPSMLFLDEPT
                     TGIDAVSRGELWDMLQKLKEKGITILVSTPYMDEASLCDRIALCNEGRILGIDSPQGI
                     VEKFNLKLYAIRADNMFTLLEAARQVEGVVECYPFGQTHHLIADEKFDIHRFTEQLEA
                     LSNLTIKPTKPAIEDVFIKLMKQ"
     gene            complement(1013..1900)
                     /locus_tag="PKY68_07305"
     CDS             complement(1013..1900)
                     /locus_tag="PKY68_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOH24875.1"
                     /translation="MKRVVYIAAIILTVSSCGKKAEFDAQGTFEAIEVVVSSEATGRI
                     LSFEIEEGMPVKAGMELGLIDSLQLHLQYKQLSSQLSALLDSRPDTKAQVAALREQIA
                     KQKRELKRVGNMLKDAAATQKQYDDIEAQIRILESQLSATLSSLDKSTATINNNAEAL
                     EAQMAALADRMAKCRIASPVDGTVLVKYAQAGELATMGRPLMKIADLNNIYLRAYFTS
                     NQLAHIKLGDEVIVTADFGGDERYDYAGRITWISPESEFTPKTIQTKDSRANLVYAVK
                     IAVKNDGRLKIGLSGNVKL"
     gene            complement(1904..3187)
                     /locus_tag="PKY68_07310"
     CDS             complement(1904..3187)
                     /locus_tag="PKY68_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH24876.1"
                     /translation="MRVFYISFLMLLSTVSIGAQTLDECRRLAREHYPEIRQYNLVTR
                     TEQFNVSNAAKAWIPQVVLSGQATYQSAVPAYPEALKNMMQANGVDMSGTGMNKDQYR
                     IAVDVTQNIWDGGQSKASRAMAEAEAAEQRSRADVSLYDLQSRVDGLYFGILLLDERM
                     EQTKAMIEVLGSNLSRMRTYHENGVALQSDVDVMEAELLTAQQQLGQVESSLTSYRRM
                     LEIFIGQSLISDKLERPAMPIIQGYTSTRPEFALLDAQENRLTAQRRAINSSVMPRFG
                     AFAQGYYGYPGLDIFKDMTSTEWTLNGIVGVRMLWNISAFYTKNNNLEKINIAQQQLA
                     VQREILQFNTRMLTTQDDGEIARLRKAVENDARIVELRRSVRMAAESQLKNGVIDATD
                     LLRKITDETTAALNRNVHEIELLQAIYKLKHTLNE"
     gene            complement(3269..>3581)
                     /locus_tag="PKY68_07315"
     CDS             complement(3269..>3581)
                     /locus_tag="PKY68_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH24877.1"
                     /translation="MNVFIFLFMLYGFYSALTDGSQKAMISDIVSKELKGTGFGIYHA
                     VLGITLLPASLIAGLLYDRVNSNAPFYFGSAMAFTATVLMIIFTVLGKRKQKLTNGLQ
                     R"
BASE COUNT          957 a          857 c          738 g         1029 t
ORIGIN      
        1 cacctagaaa accaaaaatt tcacccttgt aaacatcaaa agagatattg tttacagcaa
       61 tgaaattgcc aaaacttttg gtaaggttgc gtactgaaat tactgtttcg ctcattgttt
      121 catcagcttt ataaatacat cttcaatagc aggtttcgta ggtttaatag taagattact
      181 caaggcttcc aactgctctg taaagcggtg tatatcaaac ttctcatctg caataaggtg
      241 atgcgtttgt ccaaacgggt aacactctac cacaccctca acttgtcgtg ctgcttcgag
      301 cagagtaaac atattgtcgg cacgaatagc ataaagcttc aaattgaatt tttcaacaat
      361 tccttgcggg ctgtctattc ccaaaatacg tccttcgtta catagtgcaa tgcggtcaca
      421 aagtgaagct tcgtccatat aaggagtaga taccaggatg gtaatccctt tctctttgag
      481 tttttgtaac atatcccaaa gttcaccacg cgatacggca tcaatacctg ttgtcggttc
      541 gtcgagaaag agcatgctcg gatggtgaat taatgcacaa gagagagcga gtttttgttt
      601 catgccaccc gatagtttac ccgcctggcg tgtacgaaaa ggttcaattt gtttatagat
      661 cggagcaatg aggtcgtatg aatcttcaac cttaacgcca aagagtgagg caaaaaatat
      721 aagattttct tccaccgtca ggtccggata aagtgaaaat cgaccgggcg tataacccac
      781 tcgcgaacgt atcttaagat agtcttttat aacgtctaaa ccatcgatcg atgctgtgcc
      841 tgtatcggga ttgagcaatg tagtaagcag acgaaaaagc gtggtcttgc ctgcaccatc
      901 aggacctata agaccaaata gttcgccccg cttaaccgaa aacgagaccg aatcgagagc
      961 tttaactttt ccgaaactct ttgaaagacc gcttacatct atcacatcca tactacaatt
     1021 tcacattgcc cgacaatccg attttaaggc ggccatcgtt ctttacggca atttttacgg
     1081 catagaccag atttgcccgc gaatctttcg tctgtatggt ttttggcgta aactcacttt
     1141 cgggcgaaat ccatgtaata cgacccgcat aatcgtaacg ttcatcacct ccgaagtcgg
     1201 ctgtaactat aacttcgtcg cccagtttta tgtgggcaag ttgattggat gtgaaatagg
     1261 cacgaagata aatgttgttg agatcggcaa ttttcatcaa gggcctaccc attgttgcca
     1321 actcacctgc ttgggcatac ttcaccagta ccgtaccatc aacgggcgac gctatccggc
     1381 attttgccat acggtcggca agagcagcca tctgtgcttc gagagcttcc gcattgttgt
     1441 tgatggtagc ggtgctttta tcgaggcttg agagcgttgc cgaaagttga ctctcgagaa
     1501 tacgtatctg tgcttcaatg tcgtcgtatt gtttttgcgt ggcggcagcg tctttgagca
     1561 tgttccccac tcgcttaagc tccctctttt gtttggctat ctgctcacgt agtgctgcga
     1621 cttgcgcctt tgtatccgga cgactgtcca atagtgccga aagttgtgac gacaactgct
     1681 tatactgaag atggagctgc agactatcga tcaagcccag ctccatgcct gctttaacgg
     1741 gcataccttc ttcaatttca aaactcaaaa tgcggcctgt agcttccgat gacaccacca
     1801 cttcgatagc ctcaaaagta ccctgcgcat caaattccgc ttttttaccg cacgatgata
     1861 cggtaagtat tattgcggcg atataaacca ctcgtttcat atcttattcg tttaatgtat
     1921 gtttcaattt ataaatagct tgtagcaatt ctatctcgtg tacattacga ttgagtgccg
     1981 ctgttgtttc gtcggtgatt ttacgcagta aatccgtggc atcgataacg ccgtttttaa
     2041 gctgtgattc cgcagccatg cgtaccgagc gacgtaattc cacaatacgg gcatcattct
     2101 caaccgcctt acgaagtcgg gcaatctcgc catcgtcctg tgtggtcagc attctggtat
     2161 tgaattgtaa tatttcgcgc tgaacggcta attgttgttg agcaatgttt attttttcaa
     2221 gattattatt tttggtgtag aatgcgctta tattccacaa catgcgcacg cccacgatgc
     2281 cattcagggt ccattccgta gaggtcatgt ctttaaatat atccaggccg gggtagccgt
     2341 agtagccttg cgcaaaggct ccaaaacgag gcatcaccga tgagttgata gcccttcttt
     2401 gcgcagttaa cctgttttcc tgagcatcga gcagagcgaa ttcggggcgt gttgaagtgt
     2461 agccctgaat aatcggcatg gctggccgtt ctaacttatc ggaaatcagc gactgaccga
     2521 taaaaatctc caacatacgg cgatagcttg ttagggagga ttccacctgg cccaactgtt
     2581 gctgagcggt aagcaattct gcctccatca catcaacatc cgattgcaac gcaacgccgt
     2641 tttcatgata ggtacgcata cgactcaggt tgctccccaa tacctcaatc atagccttgg
     2701 tttgttccat acgttcatcg agcagtaaaa tgccaaagta cagaccatcc accctcgact
     2761 gcaaatcgta gagcgataca tctgcacgac tgcgctgttc ggctgcttcc gcttcggcca
     2821 tagccctact cgcttttgat tgaccgccgt cccaaatgtt ttgagtaaca tcaacagcaa
     2881 tcctgtactg gtctttattc atacctgtac ccgacatatc cacaccattg gcttgcatca
     2941 tgtttttgag ggcttcggga taggcgggaa ctgccgactg atacgttgct tgtcccgaca
     3001 aaaccacttg tggtatccat gcttttgccg cattcgatac attaaactgt tctgtccggg
     3061 taacaagatt gtattgcctg atttcgggat aatgctcccg tgcgagacga cggcactcat
     3121 ccagagtctg agctccaatg cttaccgtag acagcagcat taaaaaactt atataaaata
     3181 ctctcatgaa attatttttt tattatccga ttcataattg atttgccacc ttagaaaagc
     3241 taaggttttc ggctaccggc ataaaaacct atctctgtaa tccattagtt aatttttgtt
     3301 ttctcttacc caaaacagtg aatatgatca tcaaaacggt ggcagtgaat gccattgcag
     3361 acccaaaata aaaaggtgca tttgagttaa ccctgtcgta gagtagtccg gcgattaaac
     3421 tcgcaggcaa caaggttatt cccaacacag catgataaat accaaacccc gtacctttca
     3481 attctttact tacaatatcg gaaatcattg ccttctggct gccatcggtc aatgcactgt
     3541 aaaatccata cagcataaac aagaaaataa aaacattcat a
//
LOCUS       DAOSGL010000236         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24290_length_3564_cov_4.405244, whole genome shotgun sequence.
ACCESSION   DAOSGL010000236 DAOSGL010000000
VERSION     DAOSGL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24290_length_3564_cov_4.405244"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PKY68_07320"
     CDS             <1..1174
                     /locus_tag="PKY68_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24878.1"
                     /translation="EYLGASGTYQRNLELVDLRWENTTQANIGLNFASFKNRLGFDFE
                     WYHKETRDLFMYNLKIPTSTGFSSVNTNVGRMDNNGWEFQCFATPVKTKDWTLTLNLN
                     FARNDNRLREISDLYPQESGISTSNASYISQLIVDQPLGSFYGYLYDGVYLNEDQTIA
                     RDKKGDKIYTYNDKGEKEPVYMRFSYPSIDYQFQPGDARYVDVNHDGNIDYQDIVWLG
                     NAMALFSGGFGGKLSWKQFTVDAFFNFRYGNDVINFAKLNMENMYAYDNQSLSVLRRW
                     RHVYEDESEAPDDLLPRALYRKGYNYLASDRFVEDGSFVRFKSLTFRYNMSRDQLKNT
                     FLQRASFFITLQNLYVWTAYTGMDPEVNISNPLSAGYDYASTPRPKDYMIGFSLTF"
     gene            1189..2781
                     /locus_tag="PKY68_07325"
     CDS             1189..2781
                     /locus_tag="PKY68_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24879.1"
                     /translation="MKKSNKILALLLPLLLMSSVSCSDWLNLLPEDSVPKQSYWKSKE
                     DVVSALKGIYSSMLSSDLSQKIWLWGEIRADMITDGRSVNASLNLVRQGEISADNSLC
                     NWSSFYTTINNCNILLRFAPEAYEKDESFTQRELKSYMAQAVAVRALMYFYLVRTFGD
                     VPFSLEAYVDDSQYLPLAKSPGSVILDSLVLQLEGLRVNNSIPYQYGSAAENKGQMTR
                     YAVDALLADIYLWKEDYASCVVVCDEILNSGQFALIPVPKTEAVTDQGDTVLHPSPAG
                     IYDLFQSLYIDGNSVESIFELPFDRDITNPFYQLLVYQNRSLMPNALYINSDIFVPSA
                     KGDPEYFDIRASINSKGGYVWKMAGVTYDGNDVFTVENMTNNLIIYRLAQVYLMKAEA
                     LTQLAGQDQEKLEEAYGLLRQLRERSCAVETTELERDGSGNIDRDLLELFVMEESARE
                     LMFEGKRWFDVLRNAKRNNYSKLNYLMSIVPNSTTPDKVYSLQVKYKNFNSHYLPLPQ
                     ADIESNPLLVQNPFYDTKINKP"
     gene            2784..>3564
                     /locus_tag="PKY68_07330"
     CDS             2784..>3564
                     /locus_tag="PKY68_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24880.1"
                     /translation="MRTDQFLNKKPVFNGILIGLVVMLALFSSCKTNLDKGLYQTTAE
                     LMIDEYLESEEELSLFLNIIDIADLRGMVHAYGTYTCFAPTNRAVSEYLEDKNLDLAT
                     LSQAEAAYMVKYHFISDTLSTADFVESRLSAANMNNQFLTTKTVMDEEGNIQIIVNRK
                     AKILEANIRLGNGLVHVVDHFLSLPTQSVEESLEAMPDSVFSTMKFFVSKLKTDYNIK
                     IDDFLKDTAYITLLVQTNAAFEKAEIADIAGLLARLKENDLK"
BASE COUNT          938 a          795 c          852 g          979 t
ORIGIN      
        1 tgagtacttg ggtgcttccg gtacttatca gcgaaacctg gaactggtcg atttgcgttg
       61 ggagaacaca acccaggcca acatcggtct gaatttcgct tcgttcaaga atcgcctggg
      121 cttcgatttt gaatggtatc acaaggaaac ccgcgacttg tttatgtaca atctgaagat
      181 tcctacctct acaggctttt cttcggtcaa caccaatgtg ggccgtatgg acaacaacgg
      241 ctgggagttc cagtgtttcg ccacaccggt caaaaccaaa gattggactc taacgctgaa
      301 cctgaatttt gcccgaaacg acaaccgtct gcgcgagatt tcggatttgt atccccagga
      361 atcgggtatc agtacttcta acgccagtta catatcgcaa cttatagtgg atcagcccct
      421 gggctctttt tacggctatt tgtacgacgg tgtgtacctg aacgaagatc aaaccattgc
      481 ccgcgacaaa aaaggcgaca aaatttacac ttataacgat aagggtgaga aagagccggt
      541 ttacatgcgc tttagctacc cctccatcga ttatcagttc cagcccggcg atgcccggta
      601 tgtggacgtg aaccacgatg gtaatataga ttatcaggat attgtgtggc tgggtaatgc
      661 catggctctg ttttcgggcg gcttcggtgg gaaactcagc tggaagcaat ttactgtgga
      721 tgctttcttc aatttccgct acggtaacga cgtgatcaac tttgccaagt tgaatatgga
      781 gaatatgtat gcttacgaca atcaaagttt gtcggtgctg aggcgctgga ggcatgtgta
      841 cgaagacgag tcggaggcgc ccgacgacct gcttcccaga gctttgtata gaaagggtta
      901 taattacctg gcttcggaca ggtttgtcga agacggttct tttgtgcgtt ttaaatcgct
      961 taccttccgg tataatatgt ccagagatca gttgaagaac acctttctgc aacgggcttc
     1021 gttctttata accttgcaga atctctatgt atggaccgct tataccggca tggaccccga
     1081 agtcaatatc agtaatccct tgtctgccgg ttacgactat gccagcacgc cccggcccaa
     1141 agactatatg ataggctttt cactaacctt ttgatgcgaa cggaagttat gaaaaaatca
     1201 aacaaaatac tggctttgtt actgcctttg ctcctgatga gctctgtttc gtgcagcgat
     1261 tggttgaatt tgctgcccga ggatagtgtg cccaaacagt cttactggaa aagtaaagaa
     1321 gatgtggttt cggccttgaa gggtatttac agctctatgc tgtcgtccga tctgagtcag
     1381 aaaatctggc tttggggcga aatcagggca gatatgatta cggatggccg ttccgttaat
     1441 gcttcgctga atttggtcag gcagggtgaa atctctgcag acaacagcct gtgtaactgg
     1501 tcatcttttt atacgaccat aaataattgc aacattctgc tgagatttgc ccccgaggct
     1561 tacgaaaagg atgagtcttt tacccaacgg gagctgaaat cttatatggc tcaggcagtt
     1621 gctgtgcgcg ctctgatgta tttttactta gttcgcactt tcggtgacgt tcctttttcg
     1681 ctggaggcct atgtggacga ttctcagtat ctgcctctgg caaagtctcc cggcagtgtg
     1741 atactcgaca gcttagtgtt gcaactggag ggtcttagag tcaacaacag cattccctac
     1801 caatacggtt cggcagccga aaacaaggga caaatgactc gctatgccgt cgatgccctt
     1861 ttggccgaca tttatctttg gaaagaagac tacgcttctt gtgttgttgt ttgcgacgaa
     1921 attttgaaca gcggacaatt cgctttgatt ccggtgccca aaacggaagc cgtaaccgac
     1981 cagggcgata ccgttcttca tccttcgccg gccggtattt acgatttgtt ccaaagtctt
     2041 tatatcgacg gcaacagtgt ggaaagcatc tttgaattgc cttttgaccg ggatattacc
     2101 aatcctttct accaacttct ggtttatcaa aatcgctcac tgatgcccaa tgctctgtat
     2161 attaatagcg atatttttgt tccttccgcc aaaggagatc ccgaatactt cgatatcagg
     2221 gcttccatca attccaaagg gggttatgtc tggaagatgg ccggtgtaac ttacgacggt
     2281 aacgatgtgt ttacggtgga gaatatgacc aataacctga ttatttaccg cctggctcag
     2341 gtttatctga tgaaagcaga agccttgact caactggccg gacaagatca ggaaaagctc
     2401 gaagaagctt acgggctgct ccgtcagttg cgtgaacgtt cttgtgcggt cgaaaccacc
     2461 gagcttgaaa gagatggttc gggcaatatt gatcgtgacc tgctggaact ttttgtcatg
     2521 gaagagtctg cccgtgaatt gatgttcgaa ggcaaacgct ggtttgacgt gctgagaaat
     2581 gccaaaagaa acaattacag caaactgaat taccttatgt ctatagtgcc caacagcacc
     2641 acacccgaca aggtgtatag tcttcaggtg aaatacaaaa atttcaacag ccattatttg
     2701 cctctgcctc aagcggatat tgagtcaaat cctttgttgg tgcagaatcc tttttacgat
     2761 accaaaataa ataagccata aacatgagaa cagatcaatt tttaaataaa aagccggtat
     2821 tcaacggaat tctgattggc ctggttgtga tgcttgcttt gttcagttct tgtaaaacca
     2881 atctggacaa aggtttgtat cagactacgg ccgaattgat gatagatgaa tacctggaaa
     2941 gcgaagagga attgtcgctg tttctgaata ttatagatat tgctgatctc aggggaatgg
     3001 tgcatgccta cggcacctac acctgttttg ctccgactaa ccgggccgtc agcgagtatc
     3061 tggaagataa aaatctcgat ctggccactc tgagccaggc ggaggctgcc tatatggtaa
     3121 aatatcattt cataagcgac actctttcga cggccgattt tgtcgaaagc cgtctgagcg
     3181 cagccaatat gaacaatcag ttcctgacga ccaaaacagt gatggacgaa gagggcaata
     3241 ttcaaatcat tgtcaatcgc aaggctaaaa tattggaagc caatatcagg ctggggaacg
     3301 gcctggtgca tgtggtggac cactttttgt cgctgccgac acagtcggtg gaggaaagcc
     3361 tggaggctat gcccgactcg gttttttcta ccatgaaatt ttttgtaagc aagttaaaga
     3421 cagactataa catcaaaata gacgatttcc tgaaagatac cgcctacatt acgcttctgg
     3481 ttcagaccaa tgccgctttc gaaaaggctg aaattgccga tattgccggt ttgctggctc
     3541 gtctcaagga aaacgacctg aaat
//
LOCUS       DAOSGL010000237         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24312_length_3562_cov_4.308811, whole genome shotgun sequence.
ACCESSION   DAOSGL010000237 DAOSGL010000000
VERSION     DAOSGL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24312_length_3562_cov_4.308811"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PKY68_07335"
     CDS             <1..701
                     /locus_tag="PKY68_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH24881.1"
                     /translation="VDELVLDLRYNSGGYVMMSTYLSSMILPALSRDKVFCYFRYNDY
                     LQAFYNKQYGEDYFLMHFKENFFPTGSSLAVLLNNLNLGRLIVLTGEYTASASEQLIN
                     GLKAYMPVILIGKTTYGKNVSSLSIYEQNNPKNRWGMQPIVAKVFNSLGESDYTTGFE
                     PDIELTELSVYDQSALGDTSELLLNAAISLILNRSVATRRSARKEIQTPVFIPDETKI
                     KGSLLPAEPDLNER"
     gene            860..2632
                     /gene="fucI"
                     /locus_tag="PKY68_07340"
     CDS             860..2632
                     /gene="fucI"
                     /locus_tag="PKY68_07340"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006005 - L-fucose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOH24882.1"
                     /translation="MTKHPKVGIRPIIDGRERGVRESLEVQTMNMAKRVAELISSTLK
                     YGDGTAVKCVIADGTIGRVPEAAACAEKFEREGVGLTISVTPCWCYGSETIDMNPHYP
                     KAIWGFNGTERPGAVYLAAAMAAHNQKGLPAFGIYGRDVQDSDDNTIPEDVKEKLLRF
                     TRAGLAVAEMRGKSYLSIGGVCMGIAGSMVNPDFFQEYLGMRNEYIDMVEVSRRIDKG
                     IYDHEEYKKAWEWTLKYCKVNEGEDFNDPQWRKTRAEKDADWEYVVKMYLVIRDLMVG
                     NPKLAELGFKEESLGHNAIVAGFQGQRQWTDYLPNCDFPEALLNSSFDWNGLRRPYIL
                     ATENDSLNGVSMLLGHLLTNTASIFSDVRTFWSPEAVKRVTGYTLEGRAAHGIIHLIN
                     SGATTLDATGQQSNKQGEPAMKPFWEINQDEVNKCLKATTWSPANCGYFRGGGYSSNF
                     LSKGGMPVTMSRINMIKGQGPVMQIAEGWTVDIQPKAYEIINNRTDKTWPTTWFAPRL
                     CNKLQFKDVYSVMNNWGANHGAINYGHIGADLITLASLLRIPVCMHNVDEGKIFRPAA
                     WSAFGMDLESSDYRACNNFGPIYK"
     gene            2687..>3562
                     /gene="fucP"
                     /locus_tag="PKY68_07345"
     CDS             2687..>3562
                     /gene="fucP"
                     /locus_tag="PKY68_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOH24883.1"
                     /translation="MTNTQVKVVTREFLLPFILVTSLFYFWGIANNMTDTLLSAFKRI
                     MSMTDVQTSLIQLSFYGSYFCFALPAALFIKRYSYKSGVILGLILYAGGAMLFYPAAQ
                     TASYGFYLFAIYVLAGGCSILETTANPYILSMGSPETATRRLNLAQAFNPLGSITGIL
                     LSQYFILSELSSADATQRAQMDPEQLRLMQTGELNAITYTYMGVGFVLFVILLIMIFS
                     RMPKNFDRDRSIQLGSTFKRLIKNKIYSWGVVAQFFYVGAQIGVWSFTIRIVMQELGI
                     NEQSAANVYLLSIICF"
BASE COUNT          957 a          800 c          836 g          969 t
ORIGIN      
        1 atgtcgacga gctggtgctg gacttaaggt acaacagcgg cggttacgtt atgatgtcta
       61 cttatttgtc cagcatgata ttgcctgcgc tgagccggga taaagttttt tgctatttcc
      121 gatacaacga ctatttgcag gcattttaca acaaacaata cggagaagat tatttcctga
      181 tgcattttaa ggagaatttt ttccctacag gctccagctt ggctgttttg ctcaacaatt
      241 taaacctggg acgcctgatc gtactgaccg gggaatatac cgcttcggcc agcgagcaac
      301 tgatcaacgg actaaaagct tatatgcctg ttattctcat tggtaagact acatatggta
      361 agaatgtgtc ttcgttgagc atatacgaac aaaacaatcc caaaaatcgc tggggcatgc
      421 agcccatcgt tgccaaggtg ttcaacagcc tgggagaatc ggattataca accggttttg
      481 aaccggatat agaattgaca gagttgagcg tttacgatca atctgctttg ggagatacct
      541 cggaattgct gttgaatgcg gccatttctt tgattttgaa cagaagcgtt gctactcggc
      601 gatcggcccg taaagaaatt caaacccccg tatttatccc tgacgagacc aaaatcaagg
      661 gctcgctatt accggcagaa cccgatctga acgaaagata ataatgcaaa aactatgttg
      721 tagaacatgg tttcatcaag gttgatactg aaaatatgga gtaattttgc aagaattctt
      781 tttttaatac gatttaggaa cgaggctaca aggagcaatt aaatatataa tcattaacaa
      841 cataaaatta caacacatta tgactaaaca tcctaaagta ggaatacgtc ccataattga
      901 cgggagagaa cgaggtgtgc gagaaagcct cgaagtgcag accatgaata tggctaaacg
      961 agtggccgaa ttgatttcgt ccacgctgaa atacggcgac ggaaccgccg tgaaatgcgt
     1021 tattgctgac ggcaccatag gccgtgtgcc cgaagctgca gcctgcgccg aaaaatttga
     1081 acgcgaaggt gttgggctca ctatcagcgt aacgccctgt tggtgctacg gaagtgaaac
     1141 catcgacatg aatccccatt accccaaggc tatttggggt tttaacggca cggaacgtcc
     1201 cggtgccgtt tatctggcag ccgctatggc cgctcataat caaaaaggtt tacccgcttt
     1261 tggtatttat ggacgggatg ttcaggattc cgacgacaac actattcccg aagacgtaaa
     1321 agaaaaattg ctgcgtttta ccagagccgg acttgctgtt gccgaaatgc gaggcaaatc
     1381 ctatctctcc ataggcggag tttgtatggg tattgccggt tctatggtga atcccgattt
     1441 cttccaggaa tacctgggta tgcgcaatga atatattgat atggtcgagg tgagccgtcg
     1501 tatcgacaaa ggcatttacg atcacgaaga atacaagaaa gcttgggaat ggacgcttaa
     1561 gtattgcaaa gtcaatgaag gagaagactt taacgatcct caatggcgta aaacccgcgc
     1621 cgaaaaagac gccgactggg aatacgtggt caagatgtat ttggttattc gcgatttgat
     1681 ggtgggtaac cccaaattgg ccgaactggg ctttaaagaa gaatctttgg gtcacaatgc
     1741 catcgtagcc ggattccagg gacaacgcca gtggacggac tacctgccca attgtgattt
     1801 tcccgaagct ttgctcaaca gttctttcga ttggaacggc ctacgcagac cttatattct
     1861 ggccaccgaa aacgactcct tgaatggcgt ttccatgctg ctgggtcatt tattgaccaa
     1921 tacggcttcc atattctcgg atgtgcgcac attctggagc cccgaagcgg taaaaagggt
     1981 tacgggctat accctcgaag gcagagctgc tcacggcatc attcacttga tcaattccgg
     2041 tgcaaccact ctcgacgcta ccggacagca aagcaacaaa cagggcgaac ccgccatgaa
     2101 acctttttgg gaaataaacc aggacgaagt aaataaatgc ctcaaagcca ccacctggtc
     2161 tcctgccaat tgcggttatt tcagaggcgg aggctattct tcgaatttcc tgagtaaagg
     2221 cggtatgcct gttaccatgt cccgtatcaa tatgataaaa ggacagggtc ccgtaatgca
     2281 aattgccgaa ggttggaccg ttgatattca gcccaaggcc tacgaaataa taaacaatcg
     2341 taccgataaa acctggccga ccacttggtt tgctccccgt ttgtgcaaca agttgcaatt
     2401 caaggatgtt tacagtgtca tgaacaattg gggcgccaat cacggggcta tcaattacgg
     2461 acatataggg gccgatttga tcaccctggc ttctctgctc agaattccgg tttgcatgca
     2521 taatgtggac gaaggaaaaa ttttccgccc ggcagcctgg agtgctttcg gaatggatct
     2581 cgaatcgtcc gattatcgtg cctgcaacaa ttttggtcca atttacaaat aactttatcc
     2641 gggggaaagc aagaatgctt tccccttact tttgcccctt acagctatga caaatactca
     2701 ggtaaaagtg gtaacaagag agttcttgtt accttttatt ttggtaacct ccctttttta
     2761 tttctggggt atagccaaca atatgacaga tacgttgctg tctgctttca aacggattat
     2821 gagcatgaca gacgttcaga catccctgat acaactgtcg ttttacggtt cttatttttg
     2881 ttttgccttg ccggccgccc tttttatcaa acgttattcg tataaatccg gtgttatatt
     2941 gggtttgatc ctttatgccg gtggtgcgat gttgttttat ccggctgccc aaactgccag
     3001 ttatggcttt tacctttttg ctatttatgt gttggccgga ggttgctcca tcctggaaac
     3061 gaccgctaat ccctacatat tgagtatggg ttctcccgag acggccaccc gcaggctcaa
     3121 tctggctcag gctttcaacc ccctgggctc gatcacggga attttgctga gtcagtattt
     3181 cattctttca gaactcagca gcgccgatgc cacacagcgc gcacagatgg atcccgaaca
     3241 attgaggctt atgcagaccg gggaattgaa tgcgatcacc tacacctata tgggggtagg
     3301 tttcgttttg tttgtcattc tcttgatcat gattttttcc cgtatgccca aaaacttcga
     3361 cagagacaga tccatacaat tgggatcgac ttttaaacga ctgataaaaa acaagatata
     3421 cagttggggt gtagtggcgc aatttttcta cgtgggagcg cagattggtg tttggtcttt
     3481 taccatccgc attgtcatgc aggaattggg tattaacgaa caatcggccg ccaacgtgta
     3541 tttgctgtcg attatttgct tt
//
LOCUS       DAOSGL010000238         3555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24362_length_3555_cov_4.167429, whole genome shotgun sequence.
ACCESSION   DAOSGL010000238 DAOSGL010000000
VERSION     DAOSGL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24362_length_3555_cov_4.167429"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="PKY68_07350"
     CDS             <1..1471
                     /locus_tag="PKY68_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH24884.1"
                     /translation="HYPQDPAFMDACDELGIFMIVATPGWQFWNRDPQFQEYVLDDVT
                     QMIRRDRNHPSVLLWEPILNETRFPLDFSMKALEITQKEFPYPGAPLAAADMHSEGVA
                     QHYGVVYGWPKDEGAAEQCIFTREFGENVDDWYAQNNNNRASRSWGELPLLVQALSLA
                     DSYDEMFRTSGQFIGGAQWHAFDHQRGYHPDPYWGGQTDCFRQPKYAYYMFKSQVSPQ
                     LEHPLIETGPMVFIAHEISPFSNADLVVFSNCDSVRLICREQDTIVKPVLHKDKGMPN
                     APVIFENVFDFWQMRELSYLQKNWQQVSFVAEGIIDGKTVCSTKKMPSRRSTKLRLRI
                     DHDGQHLIADGSDFLVVVAEVTDDNGNVRRLAKDNILFSVEGEGEIIGDASIGANPRA
                     VEFGSAPVLIRSTRQAGKIKVKARVLFEGQHSPAPAEIEFESIPARLPFNYLESYQSS
                     NQEDYRFDKGKDRVKLSEQEIKTLLKEVEQQQKDFGVEK"
     gene            1593..2279
                     /locus_tag="PKY68_07355"
     CDS             1593..2279
                     /locus_tag="PKY68_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOH24885.1"
                     /translation="MRVEFQSNTTKVKQLADALRQAIAQGEYKEGEMLPSINKLSAEL
                     NLARDTVFKAFQELKLSGAIQSAPTKGYYVSSSINHVLVMLDIFSPYKNKLYNALTEN
                     LSPNDRLDLYFHHYNEDHFNMIIKNSLGRYNKYVVMNLLNDVYSEVLNLLDRSKVLLI
                     DFGKFDKSGYAYVCQGFDTTLYDCLSSGSPLFLKYQKLNFVFRLILSTPGEEYVSFTA
                     FAKKTIWAVN"
     gene            2264..2575
                     /locus_tag="PKY68_07360"
     CDS             2264..2575
                     /locus_tag="PKY68_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOH24886.1"
                     /translation="MGSQLIERHIRKEDIRKGEAFLLVSHTDMIEAVKICRAKKLMLG
                     KDVGLVTFNDAPMLEVIDNGITCISVDFREMGLLAARFINHNERLQTYVPTRLIVRGS
                     L"
     gene            2591..3364
                     /locus_tag="PKY68_07365"
     CDS             2591..3364
                     /locus_tag="PKY68_07365"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOH24887.1"
                     /translation="MNLIIDQGNSVCKWAFFENAPGAKTDYTRLDALEPVAIFYTQPP
                     EEMLSGEIFKQFKPRAAIYSSVLNRTDDVKYEAIKQNINLCFRFDSTTPIPVKNNYSQ
                     PQSLGADRLAAAVGAWTLAPGKPSLIVDLGTAITYDLLTAEGVYEGGNIAPGIYLRMK
                     ALHENTKALPLPEISVGDFPEFGKDTPSAIKAGVLQGIFHEINGYILQHRQKHPELLT
                     FLTGGDLIYFAGKLKSDIFARPNLVLTGLHRILIDYVNS"
BASE COUNT          994 a          771 c          818 g          972 t
ORIGIN      
        1 ccactatcct caggatcctg cctttatgga tgcttgcgac gagttgggta tctttatgat
       61 agtggccacc ccgggttggc agttttggaa cagggaccct caatttcagg aatacgtgtt
      121 ggacgatgtt acacaaatga tacgccgcga ccggaatcat ccttcggtct tgctttggga
      181 acctattttg aatgaaaccc gttttccgct ggacttttct atgaaagcct tggaaattac
      241 acaaaaagaa tttccttatc ccggagcccc tctggcagct gccgatatgc attccgaagg
      301 cgttgctcaa cattacggag tggtgtacgg ttggcctaag gacgaaggag cggccgaaca
      361 atgcattttc acccgtgaat ttggcgaaaa tgtcgacgac tggtatgccc aaaacaacaa
      421 caaccgggct tcgagaagtt ggggagaatt acccctgttg gttcaggcct tgtctttggc
      481 cgattcctac gacgaaatgt tccgtaccag cggtcaattt atcggaggag ctcaatggca
      541 cgctttcgat caccagaggg gctatcaccc cgatccttac tggggtggtc agacagattg
      601 tttcaggcag cccaaatacg cttattacat gtttaaaagc caggtatcac cgcagcttga
      661 acatccactc atcgaaaccg gccccatggt ttttattgct catgagattt ctcctttttc
      721 gaatgccgat ctggtggtat tcagcaactg tgattccgtg cgtttgattt gccgcgagca
      781 ggataccatt gtaaaaccgg ttctacataa ggataagggt atgcccaatg caccggtcat
      841 attcgaaaat gtcttcgatt tctggcaaat gagagaattg agttatttac agaaaaactg
      901 gcaacaagtg agctttgtgg ccgaaggcat tatagacggg aaaacggtgt gcagcactaa
      961 aaaaatgcct tcccggcgct ccaccaaatt aagattaaga atcgatcacg acgggcagca
     1021 tttgatcgcg gacgggagcg attttctggt agtcgttgca gaagtcaccg acgataacgg
     1081 caatgtgcgc agactagcca aagataatat tttgttcagc gtcgaaggag agggcgaaat
     1141 aattggcgac gcctccattg gggccaatcc cagagcggtg gagttcggat cggcaccggt
     1201 attgattcgt tccaccaggc aagccggaaa gatcaaagtc aaggccagag ttttgttcga
     1261 aggacagcat tcaccggctc ctgccgagat tgaatttgaa agcattccgg cccgtttgcc
     1321 ttttaattac cttgaatcgt atcaatcttc aaatcaggaa gattaccggt ttgataaggg
     1381 caaagacaga gtaaagctgt ctgagcagga aattaaaacc ctgttgaagg aagtagagca
     1441 acaacaaaaa gactttggag ttgagaagta gagttttaca gattcaacgg gtatttatcc
     1501 aactaaatat gtgttcgaca tcgtcagagc caacgaagtg acaccggcag gaagtgttta
     1561 tctaactaaa tttgtgttcg actagcccaa aaatgagagt agaatttcaa tccaatacta
     1621 ccaaagtcaa gcaactggca gacgctctta gacaagctat tgcccaggga gaatacaaag
     1681 agggagaaat gctgccttcg atcaacaaat tgagtgcaga gctcaactta gccagagata
     1741 cggttttcaa agcttttcag gaattaaagc tttcgggtgc cattcaatct gccccgacca
     1801 aaggttatta cgtatcttcg tccatcaatc atgtgttggt tatgttggat attttcagtc
     1861 cttacaaaaa caaactctat aacgcgctca ccgaaaattt atcgcccaac gatcgtttgg
     1921 atttgtattt tcatcattat aatgaagatc atttcaatat gatcataaaa aacagcctgg
     1981 gacgttataa taagtatgtg gtaatgaact tgctgaatga tgtgtattcc gaagtgctga
     2041 atctgttgga tcgcagtaaa gtcttattga tcgatttcgg aaaattcgac aaatcgggtt
     2101 acgcttatgt atgccaggga ttcgatacta ctttgtacga ttgcctgagt tccggcagtc
     2161 ctttgtttct gaaataccaa aagttgaatt ttgtgttccg cttaattctg agcacccccg
     2221 gggaggaata cgtttctttc accgcttttg cgaagaaaac aatttgggca gtcaattgat
     2281 agaaagacat atccgcaaag aggatatccg caaaggcgaa gcttttctac ttgtttcaca
     2341 tacggatatg atagaggcgg ttaaaatttg cagggccaaa aaattgatgc tgggaaaaga
     2401 tgtaggcctg gtgactttta acgatgcccc catgctggaa gtaatcgata acggaataac
     2461 ctgcatttct gttgatttca gggaaatggg tttgctcgca gctcgtttta tcaatcataa
     2521 cgaacgttta caaacctatg tacctactcg tttgattgta cgcggatctc tttgaataat
     2581 agaatcaaca atgaatctga taattgatca agggaattca gtttgtaaat gggccttttt
     2641 cgaaaatgct cccggggcaa aaacggatta tacacggctt gatgcccttg agcctgttgc
     2701 cattttttac actcaacctc ctgaagaaat gctttccggg gaaatattta agcaatttaa
     2761 gccccgggca gccatttatt catctgtgtt gaatcgtacg gacgatgtca aatacgaggc
     2821 cattaaacaa aatataaacc tgtgttttcg tttcgattcg actaccccta ttcccgtaaa
     2881 gaataattat tctcaacccc aaagcttggg agccgaccgt ctggctgctg ccgtgggagc
     2941 ctggacgctg gcgcccggta aaccttcact tattgtagac ttgggtacag ccataaccta
     3001 cgatttgctc actgccgaag gagtgtatga aggaggcaat attgcccccg gtatttattt
     3061 aagaatgaaa gctttgcatg aaaacaccaa ggccttgccc ttgcccgaaa tttcggttgg
     3121 cgatttcccg gaattcggta aagatacccc gtcggccatc aaagccgggg tattgcaagg
     3181 aatttttcat gaaatcaacg gttatatact tcagcatcga cagaaacatc cggaactttt
     3241 gaccttttta accggaggcg atttaattta ttttgcaggg aaactaaaaa gtgacatctt
     3301 tgcacgcccg aatttagttt taaccggttt acacagaata ctcatcgatt atgttaactc
     3361 ctaacatccg tccaggcgta aaaatactaa tagcaggctt aatgctgttc ctgttttact
     3421 ggtcggtttc tgcccaaaac aatacctttt cgccttacag ccgttacgga tacggaatca
     3481 tttccgaacc cgcctttggc ggagcctccg gtatgggtgg aatcggttac ggtttgcgta
     3541 gttcgggcca gatta
//
LOCUS       DAOSGL010000239         3552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24386_length_3552_cov_3.796683, whole genome shotgun sequence.
ACCESSION   DAOSGL010000239 DAOSGL010000000
VERSION     DAOSGL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24386_length_3552_cov_3.796683"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKY68_07370"
     CDS             <1..448
                     /locus_tag="PKY68_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24888.1"
                     /translation="FVPSTAAVKQAVQEGRLPGIVSGSATVDDAGYFGKCTVTNADSL
                     QYYLLKYFIPMSTAVISNYPYVGWEENTAEGLPTLQSYDEPVEGGKVVTITTKIDVSD
                     AGGKLSVQELDNDGNPVGGRVDVVEDYHYFPFIFDDACVHFIKEVL"
     gene            471..>3552
                     /locus_tag="PKY68_07375"
     CDS             471..>3552
                     /locus_tag="PKY68_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24889.1"
                     /translation="MTKKIVYTLIVFLLAATGALHAQDYITGKVYELAGNERIVFPGV
                     NVMVMNEQNRLLNGTTTNQNGEYSIRIPQVTGKLKVVFSFIGMETQRFDYTGQKTLDV
                     TLKEEAMMLAEMVVSAERLEKNELGITAREQTFATQSIKMEDFIESLPVTSVEEALQG
                     RMSGVDIVAGGDPGAKSSIRIRGTATLNSNADPLIVINGVPYTTDIDDSFDFATANNE
                     DYADMLNLNPHDIESIEVLKDAASTSVYGTSGANGVLLITTKKGGRGKTRFSLSSKFT
                     AKVEPNPIPMLDGDEYVAFIQDAIWNTANARGVANSSSLLELLFDTPEINYNPDWRYF
                     DEYNADTDWLSYVKKNATISDNSFSMSGGGDKATYRFSLSYLNEGGTTLGTSLQRVTS
                     SLNIGYNFSDKLRVDADFTYSDTDKEDNWTTAVRAEALRKMPNKSPFWIDDLTGEPTS
                     NYFIRQNSEEFQGAFKGSSNFHPLIMVNDSYNNSDQKEEKMTFRMNYDISTGLIYTGY
                     VSLKFRTVNNRKFLPQAATDVTIDNNYANRSTDAYSSNLSLQTENKLIFRKNWEGLHN
                     LVATGLWRTSQSVSSNYSTEIYGASSAGMSDPVTGGAIRGLGSGDSEVRTLSGIGNFN
                     YTLLNRYIINGTLNYEGKSSLGSSNRWGLFPSGGLAWHVQEEEFMSGLPWIDQLKIRA
                     SIGQSGQAPGGTAPYVGTYNAIGEYISNPAVAPSSMQLNKLKWESSTEYDVGVDVFLL
                     NNRLSMTFDYYYKYTKDLLQTGVSIPSSTGYDGKGNTISYFNSGELSNRGWEYRIDYD
                     VYRDTDWRVSLNFNVNRNINRIELLPENLSESTYTLSNGSYAQKIIAGMPVGSFFGFH
                     YEGVYQNLEDTYAKDEDGRIMLDLQGEPIVMKNGTFTCYPGDAKYQDVNYDGLINEKD
                     ILYLGNSMPVVTGGGGFNVKYKNLSLITFFHYRLGQKIINKARMESEAMNGTDNQSKA
                     VLKRWRNEGDDTIIPRALWKYGLNYMGSDRFVEDCSFLRLKTVSLSYNLPKDYCQ"
BASE COUNT          973 a          813 c          882 g          884 t
ORIGIN      
        1 gtttgtgcct tccacagcag ccgttaagca agccgtgcaa gagggccggc tgcccggcat
       61 tgtcagtgga agcgccacgg tagacgatgc ggggtatttc ggcaaatgca cggtgaccaa
      121 cgccgattcc ctgcaatatt atctgttgaa atattttatc ccgatgagta ccgcagtgat
      181 ttcgaattat ccttatgtag gctgggaaga aaacaccgca gagggcctgc caaccttgca
      241 gagttacgat gagcctgtgg agggtggcaa agtggtgacc atcaccacca aaatagatgt
      301 gagcgatgcc ggaggcaagc tgagcgtgca agagttggat aacgacggta atcctgttgg
      361 aggaagggtt gatgtggtcg aggactatca ttatttcccc ttcatcttcg atgatgcctg
      421 tgtccatttt attaaggagg tattgtaaac ccggctagaa agattctgtt atgacaaaaa
      481 aaatagttta tactctgatt gttttcttgc tggctgccac cggagcgctc catgcgcagg
      541 actatatcac gggcaaggtt tacgaattgg cgggcaacga aagaattgtt tttcccggcg
      601 ttaacgtaat ggtgatgaac gaacaaaatc gtttgttgaa cggtaccacc accaatcaaa
      661 acggagaata ttccattcgt attccacaag tgaccggcaa attgaaggtg gtattttcct
      721 ttatcgggat ggagacccag cgctttgatt acacagggca gaaaacgctg gatgtgacct
      781 tgaaggaaga agctatgatg ctggccgaaa tggtggtatc cgcggaaaga ttggaaaaga
      841 atgagttagg tattactgcc agagagcaga cctttgccac tcaaagcata aagatggagg
      901 atttcataga atccttgccg gtgacttcgg tagaggaagc cctgcaggga cgaatgagcg
      961 gggtggatat cgttgccgga ggcgaccccg gagccaaaag ctccatcagg attcgcggaa
     1021 cggccaccct gaattccaat gccgatcccc tgattgtaat caacggagtg ccctatacca
     1081 cagatatcga cgattctttt gactttgcca cggccaataa cgaagattac gcggatatgc
     1141 tcaatctcaa ccctcacgac atcgaaagta tagaggtcct caaggacgct gcctccacct
     1201 cggtttacgg aaccagcgga gccaacggag tgttgttgat taccaccaaa aagggcggca
     1261 gaggcaaaac ccgtttttcg ctttcttcga agttcacggc caaagtggaa cccaatccta
     1321 ttcctatgtt ggacggcgat gaatacgttg cttttattca ggatgctatc tggaacaccg
     1381 ccaatgcccg gggtgttgcc aattcttcct ctttgctcga attgcttttc gataccccgg
     1441 aaatcaatta caatcccgac tggcggtatt ttgacgaata caatgccgat accgactggt
     1501 tgtcgtatgt aaagaaaaac gccacgatct ccgacaatag tttttccatg tcggggggcg
     1561 gcgataaagc cacttaccgt ttttcgcttt cttacctgaa cgagggtggt accactctgg
     1621 gaacttcgct gcagagggtt acttcgagcc tgaacatcgg ctataatttc tcggacaagt
     1681 tgagggtaga cgccgatttt acttattccg acaccgacaa ggaggacaac tggacaactg
     1741 ccgtcagggc tgaggctttg agaaaaatgc ccaacaaaag tcctttttgg atcgacgatt
     1801 tgacaggcga gcctacttcc aattatttca ttcgccaaaa cagcgaggaa tttcagggcg
     1861 cattcaaagg ttcttccaac tttcaccctc ttatcatggt aaacgacagc tacaacaaca
     1921 gcgatcagaa agaggaaaag atgactttta ggatgaacta cgatatcagt acaggtttga
     1981 tttacaccgg ctatgtctcg ctaaagttta gaacggtaaa caacagaaaa tttttgcctc
     2041 aggcggctac cgatgttacc atagacaaca attacgctaa ccgtagtacc gatgcctact
     2101 ccagcaattt atcgctgcaa actgaaaaca aactgatttt ccgcaaaaat tgggagggat
     2161 tgcacaacct ggtggccacc ggtctttgga gaacctcaca gtcggtgagc tccaattact
     2221 cgaccgagat ttacggagcc tcatcggcgg ggatgtccga tcccgtcacc gggggtgcca
     2281 tacgcggact gggctcgggc gattccgagg taagaacctt gtcgggtata ggcaacttca
     2341 attatacttt gcttaaccgc tatattatta acggtacttt gaattacgaa ggcaagtcca
     2401 gcttgggcag ttccaatcgc tggggtttgt tcccttccgg gggtctggcc tggcatgtgc
     2461 aggaagagga gtttatgtcg ggtctgccct ggatcgacca gttgaaaata cgggcaagta
     2521 tagggcaaag cggacaggcc cccggaggta ctgctcctta tgtaggcact tacaatgcca
     2581 taggtgaata catcagcaat ccggccgttg ccccttcatc gatgcaatta aacaaactca
     2641 aatgggaaag ttctaccgaa tacgatgtgg gagtggatgt gttcctgtta aataaccgcc
     2701 tgagcatgac ctttgattat tattacaaat ataccaaaga cttgttgcag accggtgttt
     2761 ccattccttc atctaccggg tacgacggca agggcaatac catctcgtac ttcaattccg
     2821 gagaactgag caaccggggc tgggaatacc gtatcgacta cgatgtatat cgtgataccg
     2881 actggagagt cagccttaat tttaatgtca accgtaacat caaccgcata gagttattgc
     2941 ccgaaaattt atcggaatca acttataccc tttccaacgg aagctatgcc cagaagatta
     3001 tcgccggtat gccggtaggt tctttttttg gcttccatta tgaaggagtg tatcagaacc
     3061 tcgaggatac ttatgccaag gacgaagacg gccggataat gctcgatttg cagggagaac
     3121 ctatagtaat gaaaaacgga acctttactt gttatcccgg cgatgccaaa taccaagatg
     3181 tcaattacga cggtctgatc aacgaaaagg acattcttta tttaggtaat tccatgcctg
     3241 tggttaccgg tggcggcggc ttcaatgtga aatataaaaa cctgagcttg attacattct
     3301 tccattatcg cctgggtcag aaaataatca acaaagccag gatggaaagc gaagccatga
     3361 acggtaccga caatcagagt aaagccgttt tgaaacgttg gcgtaatgaa ggcgacgaca
     3421 cgatcattcc gagagctttg tggaaatacg gcctgaacta catgggttcg gatcgctttg
     3481 tggaagactg ttcttttttg agactcaaaa ccgtgtcgct cagttataat ttacccaaag
     3541 actattgtca ga
//
LOCUS       DAOSGL010000240         3552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24388_length_3552_cov_2.250786, whole genome shotgun sequence.
ACCESSION   DAOSGL010000240 DAOSGL010000000
VERSION     DAOSGL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24388_length_3552_cov_2.250786"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1063)
                     /locus_tag="PKY68_07380"
     CDS             complement(17..1063)
                     /locus_tag="PKY68_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOH24890.1"
                     /translation="MKDFSSNLIQHKAGLIEAMQKLGEVPGNLTLFVVDPRGRLLGTL
                     TDGDIRRGLIAGLNLEDTVDKFMFKSFHHVNKGFSLQDFKAARDKGVRLLPLLSEQGC
                     IEKIYDLKKRRSVLPLEAVIMAGGRGERLRPLTDSTPKPLLPLGDKPIIEHNIDRLIS
                     FGIETIHISLGYLGQQLVDYLGDGSQKGIQIRYIRENQPLGTAGALSLAEKFSTEYIL
                     LMNSDLFTDADFEDLYLSTLQQKACLGVASVPYSTKVPYGIFSTRDQHITGLTEKPLY
                     THYANAGIYVLHQKVLQYIPRGRSYNMTDLIDRLLAEKQSLIHNPLVGYWIDIGQYQD
                     YTNAREIVKHLSHD"
     gene            complement(1060..2304)
                     /gene="neuC"
                     /locus_tag="PKY68_07385"
     CDS             complement(1060..2304)
                     /gene="neuC"
                     /locus_tag="PKY68_07385"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOH24891.1"
                     /translation="MRKICIVTGTRAEYGLLSPLIRAVHEDPEMELQLLVTGMHLSPE
                     FGNTWTQIRDDGFPIAKKIEMLLSSDTAVGISKSNALALISFAEALNELQPDIMIVLG
                     DRTETFAAVQAALIAGVPTGHISGGELTMGAYDDAIRHSITKMSQLHFTATEVYRRRV
                     IQLGEQPASVFNVGAPGLDNIRRLPLLSREELEQALGCRLRRRNLLITFHPVTLEQQS
                     AAGQFGELLAALDELDDCLLIFTKPNSDKDGRSIIALTDDYVARRPQTSIAFDSLGQL
                     RYLSAVRQMDAVVGNSSSGIVEVPALQVPTVNIGDRQRGRLRAPSVFDCEPRRADISR
                     ALQQALAYDRRRPLQHPYGDGHACEKIFDILKHSPAPALKKAFYDLGPDFDPFTLRFG
                     EATPDSDRGCSREASTDTNNKQ"
     gene            complement(2315..3319)
                     /gene="neuB"
                     /locus_tag="PKY68_07390"
     CDS             complement(2315..3319)
                     /gene="neuB"
                     /locus_tag="PKY68_07390"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HOH24892.1"
                     /translation="MKTLIIAEAGVNHNGDMELAKGLIEAAATAGADMVKFQSFKAEA
                     MVSKTAPKADYQKTNSGPDDSQYRMLKKLQLDRDQHLQLMAHCRLCGIQFLSAPFDLE
                     SIELLHSLAVPLYKIPSGEITNKPYLKAIASKHQPVIMSTGMSEISEIEAALAVLYEN
                     GLTKADITLLHCNTQYPTPMKEVNLRAMIQLREKLGLAVGYSDHTRGIEVPIAAVALG
                     ARVIEKHFTLDRGLPGPDHQASLEPQELKAMVVAIRNIEKALSGSGIKEPGAGEIKNK
                     DIARKSIVAARAICRGEVLTEQNLGVKRPGNGISPMRWDEVLGQRAVRDFEQDELIEL
                     "
     gene            complement(3322..>3552)
                     /locus_tag="PKY68_07395"
     CDS             complement(3322..>3552)
                     /locus_tag="PKY68_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOH24893.1"
                     /translation="ALVEHEARIGAFCHISTAACINGQVSIGEHCFVGSNTVVGNNLD
                     ITDHCVLAAGSQVLKNISLPGVYLGQPLRKIE"
BASE COUNT          657 a          916 c         1097 g          882 t
ORIGIN      
        1 catagatttg ctgtgcttag tcgtggctga ggtgttttac aatttcacgg gcattggtat
       61 aatcctgata ctggcctatg tctatccaat agcccaccaa ggggttgtga atgaggcttt
      121 gcttttcggc cagcaggcgg tcgatcaggt cggtcatgtt gtaggaccgg ccccggggaa
      181 tgtattgcaa tactttttgg tgcagcacat agatgccggc gttggcgtaa tgggtgtaaa
      241 gaggtttttc ggtcaggccg gtgatgtgct ggtcgcgggt gctgaagatg ccgtagggca
      301 ctttggtgct gtagggcacg ctggccactc ctaggcaggc tttttgttgc agggtgctca
      361 ggtagaggtc ttcgaaatcg gcgtcggtaa acaaatcgct gttcatcagc aggatgtatt
      421 cggtgctgaa tttttcggcc agcgacaggg cgccggcggt gcccaggggc tgattttcgc
      481 ggatgtaacg tatctgtatg cctttttggg agccgtcgcc caggtaatcg accagctgct
      541 gacccaggta gcccagcgaa atatggatgg tttcgatgcc aaagctgatc aggcggtcga
      601 tgttgtgctc gatgatgggt ttgtcgccca gaggcagcag gggttttggg gtgctgtcgg
      661 tgagggggcg caggcgctct ccccggccgc cggccatgat gacggcctcc aggggcagca
      721 cactgcgtcg ttttttgagg tcgtagattt tttcgatgca gccttgctcg ctgagcaggg
      781 gcagcagacg tacgcctttg tcgcgggctg ccttgaaatc ctgcaggctg aagcctttgt
      841 tcacgtgatg gaaacttttg aacataaatt tatccactgt atcttccagg ttcaggccgg
      901 caatcaggcc ccggcggatg tcgccgtcgg tgagggtgcc cagcaggcgt ccccgggggt
      961 cgaccacaaa gagcgtcagg ttgccgggga cttcgcccag cttttgcatg gcttcgataa
     1021 ggccggcctt gtgttgtatc aggttggagc tgaaatcttt cattgcttgt tgtttgtgtc
     1081 ggttgatgct tcgcggctgc agcctcggtc ggagtcgggt gttgcttcgc cgaagcgcag
     1141 ggtgaaggga tcaaagtccg ggccgaggtc gtagaatgct ttcttgagcg cgggggccgg
     1201 gctgtgtttc agtatgtcga aaatcttttc gcaggcatgc ccgtcgccgt aaggatgttg
     1261 caggggcctg cggcgatcgt aggccagggc ctgttgcagg gcgcggctta tgtcggcccg
     1321 gcggggttcg cagtcgaaga cggagggggc gcggaggcgt ccgcgctggc ggtcaccgat
     1381 gttgaccgtg ggcacctgca gggccggcac ttcgacgatg ccgctcgagg agttgcccac
     1441 cacggcgtcc atctgccgga ctgcagacaa atagcggagc tggcccagcg aatcgaaggc
     1501 gatggacgtt tggggacgac gggctacgta gtcgtccgtc agggcaatga tgctgcggcc
     1561 gtctttgtcg gaattgggtt tggtaaaaat cagcaggcag tcgtccagct cgtcgagggc
     1621 ggccagcaat tcaccgaatt gcccggcagc gctttgttgc tccagggtga cgggatggaa
     1681 ggtgatgagc aggttgcggc ggcgcagcct gcagcccaaa gcctgctcca gctcttcgcg
     1741 gctcagcagg ggcaggcggc ggatgttgtc caggccgggg gcgccgacgt tgaagacgct
     1801 cgcgggctgc tcgcccagct gaatgacccg gcgccgatag acttcggttg cggtaaaatg
     1861 cagctggctc atcttggtga tgctgtggcg gatggcgtcg tcgtaagctc ccatggtgag
     1921 ctctcctccg gagatgtgtc ctgtaggcac gccggctatc agtgcagcct gcacggcagc
     1981 aaaagtttcg gtgcggtcgc ccagcacaat cataatgtcg ggctgcagtt cattcagtgc
     2041 ttcggcaaaa gaaatcaggg ccagggcatt ggatttgctg atgcccaccg ccgtatcgga
     2101 cgagagcagc atctcgattt ttttggcaat gggaaagccg tcgtcgcgga tttgggtcca
     2161 ggtgttgcca aactcgggcg aaaggtgcat gccggtcacc agcaactgca gttccatttc
     2221 gggatcctcg tgaacggccc tgatcagcgg gctgagcagg ccgtattcgg cccgggttcc
     2281 tgtgactatg caaattttcc tcatggcttt ttatttagag ttctatcagt tcgtcttgtt
     2341 cgaaatcgcg aacagcccgc tgccccagca cctcgtccca gcgcatggga ctgatgccgt
     2401 tgcccggacg tttcactccc aggttttgtt ccgtcaacac ttctccccga cagatagccc
     2461 ttgccgccac tatacttttg cgggcaatgt ctttgttttt gatctcgccg gcgcctggtt
     2521 cttttatgcc tgaacccgac agggcttttt cgatattgcg tatggcgacc accatagctt
     2581 tcaactcctg aggctccagc gatgcctgat ggtcggggcc gggcaaaccg cggtcgagcg
     2641 taaagtgttt ttcgatcacc cttgccccca gagccaccgc cgctatggga acctcaatac
     2701 ccctggtgtg gtcggaataa cccacggcca gacccagctt ttcgcgcagc tgaatcatag
     2761 ccctcaggtt gacctctttc atgggcgtgg ggtattgggt gttgcaatgc aacagagtga
     2821 tatcggcttt tgtcagaccg ttttcgtaca acacggccaa ggctgcttcg atttcggaga
     2881 tttccgacat cccggtgctc ataatgaccg gctggtgttt ggaggctatg gcttttaagt
     2941 agggcttatt ggtgatttcg cccgaaggaa ttttatacaa agggacagcc agactgtgca
     3001 gcaattctat actttccaga tcgaagggag ccgaaagaaa ctgaatgccg cagagccggc
     3061 aatgagccat caattgcaag tgctgatcgc gatccagctg cagttttttg agcatccggt
     3121 actgagaatc gtcgggcccg gagttggttt tttggtaatc ggctttagga gctgttttac
     3181 tcaccattgc ttctgccttg aagctttgaa atttcaccat atcggcaccg gcagtggccg
     3241 ctgcttctat caggcctttg gccagctcca tatcgccgtt gtgattgacg cctgcttcgg
     3301 ctatgatcag tgttttcatc gctattctat ttttcgaagg ggttgtccca ggtaaactcc
     3361 cggcaggcta atgtttttta atacctgaga gccggcagcc agtacacaat gatctgtaat
     3421 atccaggttg ttgcctacca ccgtattgct gcctacaaaa caatgctccc ctatgctaac
     3481 ctgtccgttg atacaggcag cagtggaaat atggcaaaag gctccgatcc gggcttcgtg
     3541 ttcgaccagg gc
//
LOCUS       DAOSGL010000241         3549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24423_length_3549_cov_3.500572, whole genome shotgun sequence.
ACCESSION   DAOSGL010000241 DAOSGL010000000
VERSION     DAOSGL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24423_length_3549_cov_3.500572"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PKY68_07400"
     CDS             complement(<1..1057)
                     /locus_tag="PKY68_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOH24894.1"
                     /translation="MKKLVVYTTFILLFCFCVRAELTVIRAIPPDGSTGVDINSRVVL
                     VFDKNIAEDRGIACSLNGTVVVPAVSSKMATFILPCMDYSVSYQFHAPAGAFIEKDNP
                     SNLSPEIKLTFTAKSRPQPEARLFDAIVSADSTGDYISVQAAINAAPEKRSKPWLIFV
                     RNGVYNEVIRVPESKPYIHLIGENADSTIIRYKICCTFLVDGGYTGPLEEAEAFGAYA
                     AQFSAGYARGSLGIDAMTHIDAKNFYSENISYINLWGYEERNYPQALAVWCTKDRTAF
                     YNCKALSYQDTWRTPSSGGYRQYLKNCLIEGNVDFIYDNGDVFFDSCQINIIKNDKAT
                     EEAGWIVAPSHSGTKWGY"
     gene            complement(1101..2882)
                     /locus_tag="PKY68_07405"
     CDS             complement(1101..2882)
                     /locus_tag="PKY68_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24895.1"
                     /translation="MKKYLTILSLSAILGLTSCEAMFITDPDDIINVDDYISEDDEMY
                     KGFLGIMTRMQQSADHSIILTDTRGDYFEVTPNAPLELQKIYSYEPTDGNSYADPAPY
                     YAVVIACNDYIDKMRDYREKAGENMDNNSSRNFNALISSTIRLKVWTYLKLGSIYGEA
                     IWFDNPLEEKIDLSDHTVFSRCNMNQIVNRCLHLLDSGLVLYKNEVISSKIIMDWASW
                     INEEKVTDNYIHWQYMIPEYLPLRCELLLWRGQEEDFRWVRDNIMDYLFKVHMGTIND
                     GVNVAFNDFRYACNIPIRTGADNLYATEYYRMFYNELYNASNFTNFYQVITGIMYDYK
                     NHQTNRIIEYFCPRSPGKYYLRPSSYAVAKYPESDIRGIVQRNNMDVIDGDTCFCKYY
                     YHRGEWLRTRIVEIHPVIPLFRGHDYHFYLAEAENHLHQWHQAEVIMNAGVTNEFADK
                     ILPNYWNSNYSTWFCPAGGYGDIGVAGCVYGKMRDYPVRNLVKTDKTSDGTGPFYNSK
                     NEVFDEDARMREYDLLIMDEALAEYAGEGRAYAIVCRMAERYGAEAVVDRIADKYKGG
                     PYYDQVRNAVLSGDYWVRWDLKADDLN"
     gene            complement(2888..>3549)
                     /locus_tag="PKY68_07410"
     CDS             complement(2888..>3549)
                     /locus_tag="PKY68_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HOH24896.1"
                     /translation="YGYRTNGIYETSAEARADNYTSVYNSKYKAGDIRFVDRHADGII
                     NEQDKQLLGSTSPDCFGSLFTQLRFRQISLNAQFNYRIGGKIYNAVRRQLESMDNFHN
                     QSVAVLNRWQAEGQETTMPRAVYGDPSGNNVFSDRWIEDGSYIKLGSLSLSYSIDQPI
                     GKLFRSGTVWISGENLLTFTNYLGNDPELAYNYDEALYGMDFAKVANPITLKIGFNLN
                     F"
BASE COUNT          841 a          796 c          925 g          987 t
ORIGIN      
        1 cataacccca tttggtccca ctgtgcgagg gcgctacaat ccagccggct tcttcggtgg
       61 ctttgtcgtt ttttataata ttgatctggc aggaatcaaa aaagacatcg ccgttgtcgt
      121 agatgaaatc gacgtttcct tcgatcaggc aatttttgag gtattggcgg taaccgccgc
      181 tggagggtgt acgccaggta tcctggtaac tcagggcctt gcagttgtaa aaggcggtgc
      241 ggtctttggt gcaccaaacg gccagagcct gaggataatt gcgttcttcg tagccccaga
      301 gattgatgta agagatattt tcggaataaa aatttttggc atctatatgg gtcatggcgt
      361 caattccgag agagcccctg gcatagccgg cgctgaattg agcagcgtag gccccaaagg
      421 cttccgcttc ttccaaaggc cctgtgtagc ctccgtccac caaaaaagtg cagcagattt
      481 tataccggat aatggtactg tcggcatttt cacctataag atgaatgtat ggtttggatt
      541 ccggcaccct tatcacttcg ttataaactc cgttgcgaac aaaaatcaac cagggtttgg
      601 agcgtttttc gggagccgca ttgatggctg cctgtacact gatataatca ccggtactgt
      661 ctgccgagac gatggcgtca aaaagcctgg cttcgggttg aggcctgctt ttggcggtaa
      721 atgtcagttt gatttcggga gaaagattcg aaggattatc tttttcgata aaagctccgg
      781 caggagcgtg gaattgataa ctgacggaat aatccataca gggaaggatg aaggtggcca
      841 ttttcgagga aacagcgggt acgacaactg tgccgtttaa gctgcaggcg atgcctcggt
      901 cttcggcaat gttcttgtcg aagacaagca ccacccggct gttgatgtcg actccggtgc
      961 tgccgtcggg gggtatggcc ctgataacgg ttagttcggc ccgaacacaa aagcaaaaca
     1021 gcagaatgaa tgttgtgtaa acgacaagtt ttttcatacc tacatggttg tggacggatt
     1081 taatgtccgg attctcaaaa tcagttcaga tcgtctgctt taaggtccca tctgacccaa
     1141 tagtcgccgc ttagaacagc gttgcgaacc tggtcgtaat aggggccgcc cttgtattta
     1201 tcggcgatac gatctaccac cgcctcagcg ccgtaacgtt cggccatacg gcaaacaata
     1261 gcgtaggctc tgccttctcc ggcgtattct gccagagctt cgtccataat gagcagatcg
     1321 tactcacgca tgcgggcatc ttcgtcaaaa acttcgtttt tactgttgta gaaagggcct
     1381 gttccgtcgg aagttttatc ggtcttcacg agattgcgaa ccgggtaatc acgcattttt
     1441 ccgtaaacac agcccgctac gccgatatct ccataaccgc cggcagggca gaaccaggtg
     1501 gagtaattgc tgttccagta attgggcagg attttgtcgg caaattcatt ggtaacaccg
     1561 gcattcatta tcacttcggc ttgatgccac tgatgcagat gattttcggc ttcggccagg
     1621 tagaaatggt agtcgtgccc cctgaacaag ggaatgaccg ggtgtatttc cactatacgg
     1681 gtacggagcc attcaccccg gtgataataa tacttgcaga aacaagtatc tccgtctatt
     1741 acgtccatat tgttgcgttg tacgatacct ctgatgtccg attcgggata ctttgccacg
     1801 gcgtaggagg aagggcgtaa ataatatttc ccggggctgc gcgggcaaaa atattctatg
     1861 atacgattgg tctgatggtt tttgtaatcg tacatgatgc cggtgattac ctggtaaaaa
     1921 ttggtaaagt tgctggcatt gtacagctcg ttgtagaaca tccggtaata ttcggtagcg
     1981 taaaggttgt ctgccccggt ccggatgggg atgttgcagg catagcggaa atcgttaaag
     2041 gctacgttaa caccgtcgtt gatggttccc atgtggactt tgaacaggta gtccattatg
     2101 ttgtctctta cccacctgaa gtcctcttcc tgtccccgcc agagcagcaa ttcgcagcgt
     2161 aaaggcaagt attcgggaat catatattgc cagtgtatgt aattatcggt cactttttct
     2221 tcgtttatcc acgaagccca atccataatt attttggaag aaatcacttc gtttttatag
     2281 agcaccagtc cgctgtccag caaatgaaga caacggttga cgatttggtt catgttgcaa
     2341 cggctgaata cggtatggtc ggataaatct atcttctctt cgagcgggtt gtcaaaccag
     2401 atcgcttcgc catagatgga gccgagttta agataggtcc agacttttag gcgtatggtg
     2461 ctgctgataa gggcattgaa attcctcgag gaattattgt ccatgttttc gccggctttt
     2521 tcgcggtagt cccgcatttt gtcgatgtag tcgttacagg ctatgaccac tgcataatag
     2581 ggagccgggt cggcatagga attgccatcg gtcggttcgt aactgtaaat tttttgcaat
     2641 tcgagcggcg cgttcggagt gacctcgaaa taatcccctc tggtatcggt caggatgatg
     2701 gaatggtcgg ccgactgctg catgcgcgtc ataatgccga gaaagccttt gtacatttca
     2761 tcgtcttcgc tgatataatc gtctacgttg atgatatcgt ctgggtcggt gatgaacatt
     2821 gcttcgcagg atgtcaagcc taaaatggcg gacagactca ggatggtcag gtattttttc
     2881 atagggctta gaagttcaag ttgaatccga ttttaagagt aataggattg gccactttgg
     2941 cgaaatccat accgtaaaga gcttcgtcgt agttgtaagc caattcgggg tcgttgccca
     3001 ggtaattggt aaaggtcagc aggttttctc cgcttatcca gacggtaccg gaacggaaca
     3061 gtttgccgat aggttgatca atggaatagc ttaggctgag gctgccgagt ttaatataag
     3121 agccgtcttc gatccagcga tcggaaaaaa cattgtttcc actggggtca ccgtagacag
     3181 cacggggcat ggtggtttcc tgtccttctg cctgccagcg gttgagcaca gccaccgatt
     3241 ggttatgaaa attatccatc gattccagtt ggcgcctgac ggcattataa attttgcctc
     3301 cgatgcggta attaaattga gcgtttaaac tgatttgccg gaagcgcagt tgggtgaaaa
     3361 gcgaaccgaa acaatcgggg ctggtactgc ccagcaactg tttgtcttgt tcgttgatga
     3421 ttccgtctgc gtgccggtcg acaaagcgga tatctccggc tttgtattta ctgttgtaca
     3481 cagaggtgta gttgtctgcc cgggcttccg cgcttgtttc gtatattcca ttcgtcctgt
     3541 atccgtaaa
//
LOCUS       DAOSGL010000242         3537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24526_length_3537_cov_2.724296, whole genome shotgun sequence.
ACCESSION   DAOSGL010000242 DAOSGL010000000
VERSION     DAOSGL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24526_length_3537_cov_2.724296"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKY68_07415"
     CDS             <1..357
                     /locus_tag="PKY68_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOH24897.1"
                     /translation="QLATNSLINYTKSEVRRVDVSVGIRYDESINKVRKLLLDLASAY
                     PEVLKEPAVPVVHVTSMGESSVNLQLRLWAKTEDYWPLYFSLNQAVLDEFSKQDIKTP
                     FNQLSVRFEEGVSPTK"
     gene            369..2009
                     /locus_tag="PKY68_07420"
     CDS             369..2009
                     /locus_tag="PKY68_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOH24898.1"
                     /translation="MKSKFLLLALTAGLLFSFAPERKIVVYTIGDSTMANKPLDNMSL
                     ERGWGQVLSAYLDSRYVEVDNHARNGRSSLSFRNEGLWAPIYDKIKPGDYVLIQFGHN
                     DEKPSPDRHTEPGSTFNEQLRRYVLETREKGGVPVLLTPIVRRKFDEEGRLIPTHGDY
                     PQAVRDVAAELDVILIDHNVLSRRLVQDLGPEASKELFMWVPPGTNPALPQGKEDDTH
                     LRALGAKRIARLALDEIKEKIPALAVHVREYDFVVAKDGSGDFFTVQEAINAVPDFRK
                     NGRTTIFIRNGVYKEKLILPESKINVSFIGESVEHTILTYDDYAQKLNIFGESKSTSG
                     SSSFFMYAPDFWAENITFQNSAGPVGQAVAIFVSGDRAVFKNCRFLGFQDTLYTYGKY
                     SRQYYENCYIEGTVDFIFGSSTAVFKDCEIRSKRDAYITAASTPQGRDYGYVFINCKL
                     TADPGVEKVFLGRPWRPYAQTVFIHCEMGAHIRPEGWNNWGNVSNEKTAFYAEYGSSG
                     PGADISGRVRWAKKLNKKKLAAYSIEKVLAGDDGWNPL"
     gene            complement(2006..3448)
                     /locus_tag="PKY68_07425"
     CDS             complement(2006..3448)
                     /locus_tag="PKY68_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOH24899.1"
                     /translation="MIGFVACLTALIAGYFLYGKYLEKVFQPEKTRTTPAFSLRDGVD
                     YVPMPTWKIFLIQFLNIAGLGPIFGAIMGAMYGPVVFLWITLGTIFAGGLHDYFSGMI
                     SERYNGESLPELVGRFLGIPVRTFMRFFTIILMVLVGAVFVSGPADILDRISGGIMDV
                     RWWMAIIFCYYIVSTIFPIDKIIGRIYPVFGFALFFMAVGILVTLIPNLDNLPELNRA
                     GLRNLHPEAARLPIFPMMFITVACGAISGFHATQSPLMARCMKNECDGRKVFYGAMVA
                     EGCVALIWAAAGMAFFGGLDKFQFFMSENGNSAAIAVDTISRGWLGKVGGVLALLGVV
                     AAPITSADTAFRSGRLIMSDLFHYDQKKMYKRILLSFPLFAAAFVLLQINFEIIWRYF
                     AWCNQSLAVFTLWAISVYLFREGKNYFIALLPALFMTVVCTTYILTSPEGFRLNNYLS
                     VGIGLAVAICFLVLFFKKNFKNSGFSFKKR"
BASE COUNT         1013 a          865 c          825 g          834 t
ORIGIN      
        1 caattagcca ccaattccct gatcaattac accaaatccg aagtccgccg ggtagatgta
       61 agcgtaggta tcagatacga tgaatccatt aataaagtcc gcaagttgtt actcgatttg
      121 gcttcggctt atcccgaagt cttgaaggag cccgctgttc ccgttgtaca cgtgacatcg
      181 atgggcgaaa gctcagtaaa tcttcagtta aggctctggg caaaaaccga agattactgg
      241 cccctgtact tttcgcttaa tcaggcagtt ttggatgagt ttagtaaaca ggatatcaaa
      301 acacctttca accaactgtc tgttcgtttc gaagaaggtg tttctccaac caaataatga
      361 gtgtaattat gaaatcgaaa tttttattat tggctttgac tgccggtctg ctgtttagtt
      421 ttgctcccga acggaaaata gtcgtatata ccatcggtga ttccaccatg gcaaacaaac
      481 cacttgacaa tatgtctctg gaacggggtt ggggacaggt gctctctgcc tacctcgaca
      541 gccgttacgt agaagtcgac aatcacgccc gcaacggacg cagcagcttg agttttagaa
      601 acgaaggcct ttgggctccc atttacgaca agatcaaacc gggtgattac gttttgatac
      661 aattcggaca caacgacgaa aaaccctcgc ccgatcggca taccgaaccg ggcagtactt
      721 tcaacgaaca attgcgccgg tatgtgttgg aaacccgcga aaagggaggt gttcccgtct
      781 tgcttactcc catcgtaaga cgtaaattcg acgaggaagg ccgattgatt cccacccatg
      841 gcgactatcc ccaggccgtg cgcgatgtgg ctgccgagtt ggacgtaatc ctgatcgatc
      901 acaacgtttt gagccgccgg ttggttcagg atctgggtcc cgaagcatct aaagaattgt
      961 ttatgtgggt accgcccggt accaatccgg ctcttcccca gggcaaagaa gacgataccc
     1021 atttacgtgc tttgggcgct aaacgaatag ctcgtctggc gttggacgag atcaaagaaa
     1081 agattcccgc tttggccgtt cacgtcagag aatacgattt tgtggtggcc aaggacggct
     1141 cgggcgactt cttcaccgta caggaagcca tcaacgccgt acccgatttt cgcaaaaacg
     1201 gacggacgac tatttttatt cgcaacggag tttacaagga aaagcttatt ttgcccgaaa
     1261 gtaaaatcaa tgtcagtttc ataggtgaga gtgtcgaaca caccattttg acttacgacg
     1321 actacgctca gaaattgaat attttcggtg aatcaaaatc cacctccggt tcctcgagct
     1381 tttttatgta tgctcccgat ttttgggccg agaacatcac cttccaaaat tcggcgggtc
     1441 ccgtagggca ggctgttgcc atttttgtca gcggcgacag ggccgtgttc aagaactgcc
     1501 gttttttggg ctttcaggat acgctctata cctacggcaa atacagccgt cagtattatg
     1561 aaaattgtta catcgagggt acggtcgatt ttatattcgg ttcttcgacc gctgtgttta
     1621 aagattgcga aatcagatcc aagcgcgacg cttatatcac tgcggcttct acccctcagg
     1681 gtcgggatta cggctatgtt ttcatcaact gcaaacttac ggccgatccg ggagtggaga
     1741 aagtcttttt aggcaggccc tggcgcccgt acgcccaaac cgtgtttatt cactgcgaaa
     1801 tgggtgctca tattcgcccc gaaggctgga acaattgggg aaacgtttcc aacgaaaaga
     1861 cagcctttta cgctgaatac ggctcttcgg gccccggagc ggacatatcc ggacgggtac
     1921 gttgggccaa aaaactcaac aagaaaaaac tggccgcata cagcatagag aaggtgctgg
     1981 ccggcgacga cggctggaat cccctttagc gttttttgaa actaaagccg ctattcttaa
     2041 aattcttttt gaaaaaaagc actaaaaaac agatagccac cgctaaacca atgccaacag
     2101 agaggtaatt gttcagcctg aagccttcgg gcgaagtcag aatataagtg gtgcaaacga
     2161 cggtcataaa cagagccggc aacagggcaa taaagtaatt tttgccttcc cggaacagat
     2221 aaacgcttat tgcccacaaa gtaaatacag ccaggctttg gttacaccat gcaaaatacc
     2281 tccaaataat ttcaaaatta atttgaagta agacaaaagc agcggcaaac aaggggaaac
     2341 tcagcaggat gcgtttgtac attttctttt gatcgtaatg aaacagatcc gacataataa
     2401 gtcttcccga acgaaaagcc gtatcggccg aggtgatggg ggcagccacg acgccaagca
     2461 gagcgagtac gcctcccact ttgcccagcc agccccgtga aatagtatct acggcaatgg
     2521 cggccgaatt tccgttttcc gacataaaga actgaaactt atccagtcct ccaaaaaaag
     2581 ccatacctgc ggcagcccag atcagtgcga cgcaaccttc ggccaccatg gctccgtaaa
     2641 atactttcct gccgtcgcat tcgtttttca tgcagcgggc catcagggga gactgagtgg
     2701 cgtgaaaacc cgaaatggcc ccgcaggcta cagtgataaa catcatggga aaaatgggta
     2761 aacgagctgc ttcggggtgc aggttgcgca agcctgcccg gttgagctca ggcagattgt
     2821 ccaggttcgg gataagagtc accagaatgc ccacagccat aaaaaacaaa gcaaaaccaa
     2881 agacagggta tattctgccg attattttat cgatggggaa aatagttgag acaatataat
     2941 agcaaaaaat aatagccatc caccagcgaa catccattat gcctccactt atcctgtcta
     3001 gaatgtcggc agggcccgat acgaaaactg cacccaccag caccatcagt ataatagtaa
     3061 aaaagcgcat aaaggttcgt accggtatcc ccagaaaacg ccccactaac tcgggcaggc
     3121 tctcgccatt gtagcgctcg gaaatcatcc ccgaaaagta atcgtgcagc cccccggcaa
     3181 agattgttcc caatgttatc cacaggaaga ccacaggacc gtacatggct cccataatgg
     3241 ctccaaaaat aggccccaga ccggcaatat ttaaaaattg aatcagaaag attttccacg
     3301 ttggcatggg aacgtagtcg actccgtctc gtaggctgaa ggcgggtgtt gtgcgggttt
     3361 tttccggttg gaaaactttc tcaagatact ttccgtataa aaaatagccg gcaatcaaag
     3421 ccgtaagaca ggcaacaaaa ccgatcatga taaaatttta agactatgcc gtaacaggca
     3481 atcgcaaaag tacattgaat tttattatct ttgcggacta tttaatacaa aaagcga
//
LOCUS       DAOSGL010000243         3523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24694_length_3523_cov_4.591984, whole genome shotgun sequence.
ACCESSION   DAOSGL010000243 DAOSGL010000000
VERSION     DAOSGL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24694_length_3523_cov_4.591984"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /gene="hisD"
                     /locus_tag="PKY68_07430"
     CDS             complement(<1..640)
                     /gene="hisD"
                     /locus_tag="PKY68_07430"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOH24900.1"
                     /translation="MQVINYPPESAWPELMQRPAGNDTALLEQVSGILAQIKLKGDAA
                     VREYSLQFDGYAADSLELDAEEINSSCREVDPKLKEAIQQAKRNIEAFHQAQVQAPVS
                     IEVQAGVKCWQKAQPIDKVGLYIPGGTAPLFSTVLMLAVPAQLAGCGEIVLCSPPGKE
                     GKIHPAILYTAHLCGIRRIFKIGGVQAIGAMAYGTETVPQVYKIFGPGNRYVT"
     gene            complement(649..1500)
                     /gene="hisG"
                     /locus_tag="PKY68_07435"
     CDS             complement(649..1500)
                     /gene="hisG"
                     /locus_tag="PKY68_07435"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOH24901.1"
                     /translation="MLRIAIQAKGRLYEETMALLTEANIKVDASRRTLLLPARNYPVE
                     ILFLRDDDIPQAVAGGVADAGIVGENEYVERREEVQIVQKLGFSRCRLSLAIPRDMDY
                     PGLSWFQGKKIATSYPGILKDYLQANKIESEIHVISGSVEVAPGIGLADAIFDIVSSG
                     GTLVSNRLKEVEVVMQSEALLICNPRLCEEKRALLDDLAFRFNAVRQAEGKKYIILNI
                     QNSKIPDVCRLLPGMKAPTVTPLTEEGWSSVHSVIEEKRFWEIIGKLKAIGAEGILVV
                     PIEKMIL"
     gene            complement(1730..2989)
                     /gene="nqrF"
                     /locus_tag="PKY68_07440"
     CDS             complement(1730..2989)
                     /gene="nqrF"
                     /locus_tag="PKY68_07440"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOH24902.1"
                     /translation="MILLAFNALTLVLGVALFLVVTLILVALLIYVKDKLTPSGEVDI
                     DINEGEKILKTEMGDSLLSTLSNNQVFLPSACGGGGTCGMCKCRVLEGGGSILPTETG
                     FFSRREQQEQWRLACQVKVKDNLKIQIPEEILGIKKWECEVVSNHNVASFIKEFVVKL
                     PEGEQLKFKSGSYIQIDVPKIEVDFKDMDVDDRFKSEWDKFKLWGLHMSNPEPTYRAY
                     SMANHPAEGNIIMLNIRIATPPFDKQKGDWMKVNPGVCSSYIFSLKPGDKITISGPFG
                     DFHILDTDREMLYIGGGAGMAPLRSHLMHLFHTLKTTDRKISYWYGARSKNEIFYEED
                     FRAIEKEFPNFSFHIALSEPRPEDNWTGYTGFIHQVILDNYLINHEAPEDIEYYMCGP
                     GPMANAVKVMLDNLGVPKEMLMFDDFG"
     gene            complement(3002..>3523)
                     /locus_tag="PKY68_07445"
     CDS             complement(3002..>3523)
                     /locus_tag="PKY68_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOH24903.1"
                     /translation="TISHQQGDLAIELAWCVDADAAVRQVTLRVHNTGNRSAALRWVS
                     VTEWLLGAQRSDRATVHTRRNRQRLRQPMADTGHSSGTRERDARDSSEGGPWLTTLLA
                     TQADHAGGFGQATAFGLGSGIGWLLAIVGIAAIREHMSYSKVPKPLRGLGISFIVTGL
                     MGMAFMGFMGIKF"
BASE COUNT          841 a          902 c          860 g          920 t
ORIGIN      
        1 ccgtaacata acgattgccc ggaccgaaaa tcttatagac ttggggcact gtttccgttc
       61 cgtaagccat agcgcctatg gcctgtactc cgcctatttt aaagattcgc cttataccgc
      121 acaaatgagc cgtataaaga atagcgggat gtattttccc ctctttaccg ggaggcgaac
      181 acaacactat ttctccgcat ccggccaatt gggcgggaac ggccagcatc agaacggtcg
      241 aaaacaaagg agcggttccg ccgggaatgt acaaaccgac tttatcgatg ggctgcgctt
      301 tttgccagca tttcacgccg gcttgcacct caatgctgac aggggcctgg acctgagctt
      361 gatgaaaggc ttcaatattg cgtttcgcct gctgaatggc ctctttcaat ttaggatcga
      421 cctccctgca cgatgagttt atttcttcgg catcaagctc caatgagtcg gctgcatagc
      481 cgtcgaattg gagcgaatat tccctgacgg cagcgtctcc tttgagttta atttgcgcaa
      541 gtatgcccga cacttgctca agaagagcgg tatcgttacc ggcggggcgt tgcattaact
      601 cgggccaggc cgattcggga ggatagttta taacttgcat gagaagattt ataggatcat
      661 tttttctatg ggcaccacca aaataccttc tgcgccaatg gctttgagtt taccaatgat
      721 ttcccagaaa cgtttttctt cgatcacgga atgcaccgaa gaccagcctt cttcggtcaa
      781 cggcgttacg gtgggggctt tcattcccgg caacaggcgg caaacgtccg ggattttgct
      841 gttttgaata ttcagaatga tgtacttttt cccttcggcc tgacgtaccg cgttgaaacg
      901 gaaagccaga tcgtcgagca gggctctttt ttcctcacag agacggggat tacaaatcaa
      961 tagggcttcc gactgcatga ctacttccac ttctttgagc cggttgctga ccaaagtccc
     1021 gccactgctg acgatgtcaa aaatggcatc ggccaatccg atgccgggag ccacttctac
     1081 cgaaccgctg ataacatgta tttcggactc aatcttgttg gcttgtaaat aatcttttag
     1141 aattcccgga taggaagtgg ctattttctt accctgaaac caggaaaggc ccggataatc
     1201 catgtctctg ggaatggcca gcgagagcct gcagcggcta aagcccagct tctgcactat
     1261 ctgcacttct tcgcgacgtt cgacgtattc gttctcccct actataccgg catcggctac
     1321 tccgccggct accgcctgtg gaatgtcgtc gtcgcgcaaa aacaaaattt cgaccggata
     1381 atttctggcg ggtaataaca aagtgcgcct cgaggcgtcc acttttatgt tggcttccgt
     1441 caacaaggcc attgtttctt cgtacaggcg gcctttggcc tggattgcaa ttctgagcat
     1501 attgtttatt tttctcgtca aacgaatgtt gctttgtcaa aaaaaaaggc ttactcaaaa
     1561 cggtaagcct tttatgaatg atgcagacac gatcaatcaa ccaaccgttt ttcaacgtcc
     1621 ggaatgatga tgatgtccgt ggtatctgtt caatttatcc atgtctgtgt tactagcacg
     1681 ccgcaaatgt atcaataaat tgttattgta caaaataata agcttttaat catccaaaat
     1741 cgtcgaacat caacatttct ttgggtactc ccaggttgtc gagcatcact ttaacggcat
     1801 ttgccatggg gccgggaccg cacatgtagt attcgatgtc ttcgggagct tcgtgattga
     1861 tcaggtaatt atccaaaatc acctgatgga tgaagcccgt gtatcctgtc caattgtctt
     1921 cgggacgagg ttcgctcaaa gctatatgaa aactgaaatt gggaaattcc ttttcgatgg
     1981 ccctgaaatc ctcttcgtag aaaatttcgt ttttggaacg ggcgccgtac cagtacgaaa
     2041 ttttacgatc ggtggttttt agtgtatgga acaaatgcat tagatgcgaa cgcaaaggag
     2101 ccataccggc accgcctcca atgtagagca tttcgcgatc ggtatccagt atgtgaaaat
     2161 caccgaaagg acctgaaata gtgatcttat cgccgggttt gagcgagaaa atgtaggaag
     2221 aacacacacc gggattcact ttcatccaat cgcctttttg tttgtcgaac ggaggtgtgg
     2281 caatgcggat gttcagcatg atgatattgc cttcggccgg gtgattggcc atggaatagg
     2341 cccgataggt gggttcgggg ttggacatat gcaagcccca aagcttgaat ttgtcccatt
     2401 cggacttgaa cctgtcgtcc acatccatat ctttgaagtc aacctcaatt ttgggaacgt
     2461 cgatttgaat atagctgccg gatttgaatt tgagctgttc gccttcgggc aatttcacta
     2521 caaactcctt gataaaggaa gccacgttgt gattggaaac cacctcgcat tcccatttct
     2581 taatgcccaa aatttcttcg ggaatctgaa ttttaaggtt gtccttcact ttgacctggc
     2641 aagccagtct ccattgttcc tgttgttctc tccggctgaa aaaaccggtt tctgtgggta
     2701 atatgctgcc gccgccttca agtacacgac atttgcacat tccgcaagta ccgccgcctc
     2761 cacaggccga gggaaggaag acctgattat tgctcagggt cgataacaaa ctgtcgccca
     2821 tttcagtctt aaggattttt tcaccctcat ttatatcgat gtccacttcg cccgaggggg
     2881 tcagtttgtc ttttacataa atcaacagcg caaccagtat gagggtcact actaaaaata
     2941 aggctacacc aagtacaagt gtcaaggcat taaatgctaa caaaatcatc taaaatccct
     3001 cctaaaattt aatccccata aatcccataa acgccatccc catcaaaccg gtgacgataa
     3061 aagaaatacc cagtccccga agcggtttgg gtactttgga ataactcatg tgttcgcgta
     3121 tggctgctat gccgactata gccagcagcc agccgatacc tgagcccagc ccaaaggccg
     3181 tggcctggcc aaacccgccg gcatggtcgg cctgggtggc aagcagggtg gtcagccagg
     3241 ggccgccttc gctgctgtcg cgagcgtcgc gctcacgggt gccgctgctg tggccggtgt
     3301 cggccatggg ctggcgcagc cgctgccggt tgcggcgggt gtgcacggtg gcccggtcgc
     3361 tgcgctgcgc gcccagcagc cattcggtca ccgataccca gcgcaaggca gcgctgcggt
     3421 tgccggtgtt gtgcacccgc agggtgacct ggcgcacggc ggcgtcggcg tccacgcacc
     3481 aggccagctc gatggcgaga tcaccctgct ggtggctgat ggt
//
LOCUS       DAOSGL010000244         3522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24704_length_3522_cov_4.582636, whole genome shotgun sequence.
ACCESSION   DAOSGL010000244 DAOSGL010000000
VERSION     DAOSGL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24704_length_3522_cov_4.582636"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKY68_07450"
     CDS             complement(<1..255)
                     /locus_tag="PKY68_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOH24904.1"
                     /translation="MNYSQALDYLLSQLPFFQAEGKKAYKPGLDNIRQLDAWAGHPHR
                     RFPCIHVGGTNGKGSVCHMLASVLQEAGYRTGLYTSPHLKD"
     gene            382..834
                     /locus_tag="PKY68_07455"
     CDS             382..834
                     /locus_tag="PKY68_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOH24905.1"
                     /translation="MKAIKKSLGLLFVVVLVFSSCKSNKEAYSATYERAKAKQEVQAQ
                     INEEQNERVEVIEKTGVKEAAEKIEVIGQDSENGPYGVVVGSFINRTNAENLRKKMVD
                     QGYNAVLGQNDKLMFRVIVAFYEVKADAQAKVRELSKDFPDAWILIKE"
     gene            840..1037
                     /locus_tag="PKY68_07460"
     CDS             840..1037
                     /locus_tag="PKY68_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOH24906.1"
                     /translation="MKEKIGNNAGSVWTVLSAAGGRKSLKEVKKLTKLTDKDLYAALG
                     WLARENKIVFAEEDEIFVALS"
     gene            1278..1348
                     /locus_tag="PKY68_07465"
     tRNA            1278..1348
                     /locus_tag="PKY68_07465"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1310..1312,aa:Cys,seq:gca)
     gene            1471..2088
                     /gene="msrA"
                     /locus_tag="PKY68_07470"
     CDS             1471..2088
                     /gene="msrA"
                     /locus_tag="PKY68_07470"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOH24907.1"
                     /translation="MSGASKQQTHSAQTQSAQIQPPLTQSLQTHSPQISSPQTAVNKN
                     SKTMVLAGGCFWGMEELIRKQPGVLNTTVGYTGGKVENPTYQNHEGHAEAVEIEYDPK
                     RTSYRKLLDFFFRIHNPTTLNRQGNDIGSSYRSAVFYTNEEEKQEAADFIEIVNASGR
                     WKAPVVTTLEPLKQFYAAEDYHQDYLQKNPAGYTCHLVYFDSYLK"
     gene            2373..3032
                     /locus_tag="PKY68_07475"
     CDS             2373..3032
                     /locus_tag="PKY68_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24908.1"
                     /translation="MKRINYILIIFLSLFSCDKKNTTLNDQFCKVDDPMNELVWLKSI
                     IAEAVNPITIHSCSYKNHEGFLVDFCVNCPDGLVVFFDCDSNVLCEFGGIDGRNTCPD
                     FELEVMNKELIYGDTIAANNNDLFCNVNNPLEELNWLKQLVNDNKENEVRAEIYKCIY
                     DSTEGFIIDLCVQCPDGLVEFRDCTGNILCEFGGFDGRVTCPDFELKIIKKELIWTNK
                     D"
     gene            complement(3177..3413)
                     /locus_tag="PKY68_07480"
                     /pseudo
     CDS             complement(3177..3413)
                     /locus_tag="PKY68_07480"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1034 a          684 c          773 g         1031 t
ORIGIN      
        1 gtcttttaaa tggggcgagg tgtacaagcc tgtgcgatag ccggcttctt gcaagaccga
       61 ggccagcata tgacagacgc tgcctttgcc gttggtgccg cctacgtgga tgcagggaaa
      121 acgccggtgg ggatgaccgg cccaggcatc cagttgccgg atattatcca gccccggctt
      181 gtaggctttt ttcccttcgg cctgaaaaaa aggcagttga ctcaggaggt aatccaatgc
      241 ttgcgagtaa ttcataaagg gcaaatgagt tgggtttgcg aaaatacagc tttttgaacg
      301 gatttccatt taatgtaaaa cggcttttta atttaaaata aaaatgcgta cttttgtctc
      361 gaattttaaa catttccggt tatgaaagcc attaagaaaa gccttggctt gttgtttgtg
      421 gtcgtgctgg tgtttagttc ctgcaaatcg aacaaagaag catatagtgc cacttacgaa
      481 cgtgccaaag ccaaacagga agttcaggct cagataaacg aagagcagaa tgaacgtgtg
      541 gaagtgatcg aaaaaaccgg cgtcaaagag gccgccgaaa agatagaagt catcggacag
      601 gacagtgaaa atggccctta tggggtggtt gtgggcagtt ttatcaatcg caccaacgca
      661 gagaatttac gtaaaaagat ggttgaccag ggttacaacg ctgttttagg acaaaacgac
      721 aaattgatgt tcagggtaat agttgcattt tacgaagtca aggccgatgc tcaggccaag
      781 gttcgtgaac tcagcaaaga ttttcccgat gcatggattt taattaaaga ataggtatta
      841 tgaaagaaaa aattggaaac aatgccggct ccgtttggac agtgttgagc gctgccggcg
      901 ggcgtaaaag cctaaaagaa gttaagaaat tgaccaagct gaccgacaaa gatctttatg
      961 cagctttggg ttggctggct cgtgagaaca aaatagtatt cgccgaagaa gacgaaattt
     1021 ttgtagcttt gagctaatct tgagttttag gtatgtttct ttttcaggaa atagcggttt
     1081 gtctgcttgt cgggcttagt tttgtctggc tggcacaaag gctcgtcctt ttttttagga
     1141 ctgcttctca acgacaaagc tgcaacgcgg cctgtgcttc ttgtccgctg aaggataaaa
     1201 aatgcacaga gtccttgtgc aaaactaaac tatagcatac ctttgcgctt tgaaaaatcc
     1261 ggcttgtgcc ggaagtcggt tccttgaccg agtggctagg taacggtctg caaaaccgct
     1321 tacggcggtt cgaatccgcc aggaacctct taggtaattt agtaaacgac agtctcttga
     1381 gtaaggaggc tgtttttttt gacttttgcg ataccgcctt ccgtttatga gctgtctgcc
     1441 gttgtaaaac catattttaa acaagaaacc atgtccggag catcgaagca acaaacccat
     1501 tccgcacaaa ctcagtccgc acaaatccag cctccactga ctcagtcttt acaaacccat
     1561 tccccacaaa tcagttcccc acaaacggct gtaaacaaaa acagcaaaac gatggtactt
     1621 gccggtggtt gtttttgggg gatggaagaa ttgatccgaa aacagccggg ggtgctgaac
     1681 actaccgtgg gttataccgg aggcaaggtg gagaatccaa cttatcaaaa tcacgagggg
     1741 cacgccgaag ccgttgaaat tgaatacgat cccaaacgga cgagctaccg gaaattattg
     1801 gattttttct tcaggataca caatcccacc acgctaaacc gccaggggaa cgacatcggc
     1861 agttcctacc gatcggccgt attctacacc aacgaggaag aaaaacagga agcggcggac
     1921 ttcatcgaaa tcgtcaatgc ctccggacgt tggaaagcgc ctgtggtcac caccctggaa
     1981 cctctaaagc agttttacgc cgccgaagac tatcaccagg attatttaca aaagaaccct
     2041 gccggataca catgccattt ggtatatttc gactcttatc tgaagtaata cttatccgaa
     2101 gtaatattgg tttgaagtga tattggtctg aagcaatacc gggatcggtt tttacttgtt
     2161 tttttgggaa gggcgggagc ctgttctcta attaacgtgt tgaggcagct tattgttttt
     2221 tacgtattat taaccataaa gctcaatttt acattgcttt ttttacttca tttacaacaa
     2281 attgatttgt tcgttgaatt attttatacc atgtaattaa acaccaaggt gttcttgtag
     2341 aatcaataac tcacctttaa agattttacg ctatgaaaag aattaattat atcctaatta
     2401 tttttttatc tctatttagt tgcgataaga agaatacaac actgaatgac caattctgta
     2461 aagtagatga cccaatgaat gaactagttt ggttaaagag cattattgct gaagctgtaa
     2521 atccaattac aattcacagc tgctcataca aaaatcatga aggtttctta gtcgattttt
     2581 gtgttaattg cccagacggc ttggttgtct tttttgattg tgatagcaat gtattatgtg
     2641 aatttggggg tatagatggt cgaaatacct gtcctgactt tgaacttgag gttatgaata
     2701 aagagcttat ttatggcgat accattgcag ccaataataa tgatttgttt tgcaatgtga
     2761 acaatccttt agaagaatta aattggttga aacaactagt gaacgataac aaagaaaacg
     2821 aggttagagc tgaaatttat aaatgtattt atgattctac cgaaggtttt attattgatt
     2881 tgtgcgtgca atgccctgat ggtttagttg aatttaggga ctgtactggg aatattcttt
     2941 gtgaattcgg tggttttgat ggcagggtaa cttgtcccga ttttgaacta aaaatcatca
     3001 aaaaagaact aatttggaca aataaagact aacaacagct tataatgtcg tgtataagta
     3061 atggcggggt ttgtagcata ttcgagcgtt ctacctcgca ttaagttcat cgcctcttga
     3121 taggaagtac cccgtaatcc gccacttggt tatgcaaact tttttgagac aaaaaattaa
     3181 gcgacatttg taattccttg tttttttaat ttatactgtt cccaaaattc atcaatagtt
     3241 aaatagtcta agactgaaaa tcttcttttg tgattatacc aggactcaat ataatcaaat
     3301 acatacaggc gcatttgttc tcttgtcttg agtttagttc cataaatcaa ttccgctttt
     3361 agggttttga aaaaactttc agccacagca ttatcccaac aatcgccttt acgatgatta
     3421 tttcggtgaa gaccacccag gcatttcggc gataaccacc cacttatgtt agcggtactg
     3481 cctggtgcgg caaatatata tttattttct catactttca cc
//
LOCUS       DAOSGL010000245         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24790_length_3513_cov_5.240602, whole genome shotgun sequence.
ACCESSION   DAOSGL010000245 DAOSGL010000000
VERSION     DAOSGL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24790_length_3513_cov_5.240602"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PKY68_07485"
     CDS             <1..1170
                     /locus_tag="PKY68_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH24909.1"
                     /translation="NQPLPTNGNIYLQTNLTNTDDLNNAKRLMNLNTRLGLTQPLDAI
                     YGYNNIRAQLIQAKLAYEQSQKQLKRADLDLVYNVTSVFYNLLSVQKRKELAQMNLER
                     QKEAYEIAKNKFAAGLIKEVEALQMEVDLAEAQNNYDLAIMDQSSAANRFKELIGIDI
                     KDSVVLNSVLEYEVVLIDPQKAVELALQNRLEIREQEIQIELGEMSIKRQKAEGMVKS
                     SLTAYFQKVGVSELGTDQGFLNSIDRSASDFMIRPQNYGVGLTVSVPIIDWGENKALV
                     RSAESRLQKSLYRQEEVRRSIEREVLDLSNEVISSLKRLQLLEKNLVIAEKSFEITLA
                     RFSNGDIASQELALERERLNNAYTSHLSAYINYQLRMADLMRKTFYDFQNDRPIL"
     gene            complement(1171..>3513)
                     /locus_tag="PKY68_07490"
     CDS             complement(1171..>3513)
                     /locus_tag="PKY68_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOH24910.1"
                     /translation="FIGCSELETIEFNGNTKLRIGNRAFYGIKKVRTLQVGGISIGEL
                     AFGRDFYDKDLEGLESITFSPSVKIIGDGAFRYWGSLKHLDLGTSVEFIGNHAFASCG
                     MEAIQFSPEVKFIDNYAFMECPNIKKLEIPKKTKVIGDMAFYECPKLTQVTITGGDSI
                     AEKAFFGCTALTSIELGDSLRILGDQAFEGCTNIEGHIKFPKTLEFIGWKNFLGCRKI
                     TGIDLPDGLKFIGGSAFEGCIGITKLAIPARVEYIGTKAFYGCSSIKDNLVFPATLKQ
                     LRYEAFQNCIKLQSINLGSATALETIERGAFYNCKGIKGQVRIPDAVTTVEKEAFYCC
                     DSINSLHIGASLEEIGEDAFLTYPWKNLTAITVSNSNPNYDSRDNCNAMIETATNTLY
                     KGCCNTVIPETVTAIGERAFISCGITGSFKIPDAVLLIGKEAFFGCYRMSHINLSEDN
                     NLAGIGMWAFRDCEDLKEVPYLRSINSIAFGAFQGCYSMPGIHFGPQLGTIGEQAFIG
                     CYNLQHVTFWAPAPPDVGNNAFHYSFNIPMFIRCGSTAAFQDEMPEQELFIEEGYYSL
                     RVVSDDIEMGRVFITSPYSCETDQVTVAAIAEDGYKFTCWSDGCSLSPYTFTLTEDVS
                     LVAYFTEHSELKYLVTAESNDSTKGSVTGGGSYIAQTTAELTAVPADGFEFLQWNDGN
                     KENPRTFTVTQDTTFIASFGVIGAIDENNLSNVTVFTQGNNILINNALGYDLSIYDLT
                     GRLLVNETAIVTNSLVLHIGRKGMYFVKVGKGKVQKVILK"
BASE COUNT          940 a          852 c          771 g          950 t
ORIGIN      
        1 aaccagccat tacccaccaa cggcaatatt tatctgcaaa ccaacctgac caataccgac
       61 gacctgaaca acgcaaagcg cctgatgaac ctcaataccc gcctgggttt gactcagccg
      121 ctggatgcca tttacggcta caacaacatc cgtgcgcaac ttatacaagc caaattggct
      181 tatgagcagt cgcaaaagca gcttaaacgt gccgatctcg atttggttta caatgtaact
      241 agtgtgtttt acaatttatt gtcggtgcag aaaaggaaag aactggccca aatgaacctt
      301 gagaggcaaa aagaggccta cgaaatagcc aaaaacaaat tcgcggccgg tttgatcaag
      361 gaggtagaag ccttgcagat ggaggtagat ttagccgaag ctcaaaataa ttacgacctg
      421 gccattatgg atcagagttc ggctgccaac cgttttaaag aattgattgg cattgatatc
      481 aaagacagtg tggtgttgaa ttcggttctt gaatatgaag tggtgcttat cgatcctcaa
      541 aaagccgtag agttggcttt acaaaaccgc ctcgaaatcc gcgaacagga gattcagatt
      601 gagctcggcg agatgagcat caaacgtcaa aaagccgaag gtatggtcaa gagcagcctg
      661 acagcctatt ttcagaaagt aggagtcagc gagttgggca ccgatcaggg ctttttgaat
      721 tccatcgacc gttctgcttc cgattttatg ataaggccgc agaattacgg ggtaggtctt
      781 actgtgagtg tgcccatcat cgactggggc gaaaacaagg ccttggttcg ctcggccgaa
      841 tctcgcctgc agaagagcct ttaccgtcag gaagaagtgc gtcgcagcat agaacgcgaa
      901 gtgctcgacc tttccaacga ggttatcagc agccttaaac gtttgcaatt gctcgaaaaa
      961 aacctggtga ttgccgaaaa gagtttcgag ataaccctgg cccgtttctc caacggcgac
     1021 atcgccagcc aggaattagc tctggagcgc gaaaggctca acaacgctta cacctcgcac
     1081 ctgtcggcct acatcaacta ccagttgcgc atggccgacc tgatgcgtaa aactttttac
     1141 gacttccaaa acgacaggcc gatactgtag ttattttagt atcacttttt gcactttgcc
     1201 tttacctact tttacaaagt acataccttt acgtcctatg tgcagcacaa ggctgtttgt
     1261 gacgatagct gtttcattta caagtagccg gcccgttaag tcgtagatgc ttaggtcgta
     1321 acccaaagca ttgttgatca agatgttatt accctgtgtg aacacggtca cattcgacag
     1381 gttgttttcg tcgattgcac caatcactcc gaagcttgca ataaacgtgg tgtcttgcgt
     1441 tactgtgaaa gtacgcgggt tttccttgtt accgtcgttc cattgcagaa actcaaaacc
     1501 gtcagccggc acagcggtca attctgcggt agtctgagcg atatagctgc cgccacctgt
     1561 tacgctgcct ttggtggagt cgttgctttc agcagttact aagtacttaa gttcggaatg
     1621 ttccgtaaag taagccacca atgaaacgtc ttccgtcagg gtaaaggtgt atgggctaag
     1681 tgagcatccg tcgctccaac atgtgaattt gtaaccatcc tcagctatgg cagcgaccgt
     1741 tacctgatcg gtttcgcagc tatagggtga ggtaataaac accctgccca tttctatatc
     1801 atccgacaca actctaagcg agtaatatcc ttcttcaata aagagttcct gctcaggcat
     1861 ttcatcctga aaagctgcgg tacttccgca tcggataaac atggggatat taaaactgta
     1921 atgaaatgca ttattaccca catccggtgg tgcaggcgcc cagaatgtaa cgtgctgcag
     1981 attataacaa cctataaaag cttgttcacc tatcgttccc aattgtggac caaagtgaat
     2041 accgggcatg ctataacacc cttggaaagc tccaaaggcg atagagttta tagaccgcag
     2101 atacggtacc tctttcagat cctcgcagtc ccggaacgcc cacattccga tgcctgccaa
     2161 gttattatct tcacttaggt tgatgtggct cattctgtaa caaccgaaaa aagcttcttt
     2221 accgatgagt agtactgcat ccggaatttt aaaactacct gtgataccgc agcttataaa
     2281 agccctttcc ccgatagcgg ttaccgtttc aggaataacc gtgttgcagc accctttgta
     2341 aagcgtattg gtagctgtct caatcatagc attgcaatta tcccgactgt cgtaattggg
     2401 gttgctgtta ctgacggtaa tggcagttag atttttccaa ggataggtaa gaaaagcatc
     2461 ctcaccaatc tcctccagcg atgccccgat atgcaggctg tttatgctgt cgcaacagta
     2521 aaatgcctct ttttctacag tggttacggc atccggtatt cgcacctgtc ccttaatccc
     2581 tttacagttg tagaatgcac ctctctcgat ggtttccagt gccgtggcac ttcccaaatt
     2641 gatactttgc agtttaatac agttttggaa tgcctcgtac cggagctgtt ttagcgtagc
     2701 gggaaatacc agattatctt tgatactcga gcagccgtaa aatgctttgg taccaatata
     2761 ttccacccgg gcaggtatag caagtttagt aatgcctatg catccttcga aggctgaccc
     2821 cccgatgaat ttcagtccgt caggaaggtc tatgccggta atctttctac atcctaagaa
     2881 gtttttccag cctataaact ccaaagtctt aggaaacttg atatgtccct ctatgttcgt
     2941 acacccctcg aaagcttggt caccaaggat tctgagggaa tcgcccaatt ctatgcttgt
     3001 cagcgctgtg caaccgaaga atgccttctc agcaatggaa tcgccacctg ttattgttac
     3061 ttgggtcagt ttcgggcatt cgtagaaagc catgtcgcct atgacttttg ttttttttgg
     3121 aatctctaat ttcttaatgt tcggacattc cataaatgcg taattgtcaa taaatttcac
     3181 ttcgggacta aactgaatag cctccatgcc gcaagaggcg aaagcatggt tgccgataaa
     3241 ttccactgaa gtacccagat cgaggtgctt tagtgaaccc cagtatcgaa aggcgccatc
     3301 cccgataatt tttaccgaag gagagaaagt tatactttcc agaccctcta ggtctttgtc
     3361 atagaaatct ctgccaaagg ctagttcccc gatacttatg ccgcctacct gcagggttct
     3421 tactttttta atgccgtaga atgccctatt tccaatccgt aacttggtat tgccgttgaa
     3481 ctctatggtt tccaactccg aacagccgat aaa
//
LOCUS       DAOSGL010000246         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_24935_length_3497_cov_3.807960, whole genome shotgun sequence.
ACCESSION   DAOSGL010000246 DAOSGL010000000
VERSION     DAOSGL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24935_length_3497_cov_3.807960"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKY68_07495"
     CDS             complement(<1..426)
                     /locus_tag="PKY68_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH24911.1"
                     /translation="MLEKFLSKVEFDSLDDFRRNFHINVPDSFNFAYDVADVWAREQP
                     DKRAVCWTNDQGLHHDYTFAEIKIRSDQAASFFQQMGIGHGDKVMLILKRRIEFWFCL
                     LGLHKIGAIAVPATHLLTPKDLVYRNKAASIKMVVAVGEE"
     gene            complement(429..986)
                     /locus_tag="PKY68_07500"
     CDS             complement(429..986)
                     /locus_tag="PKY68_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOH24912.1"
                     /translation="MNEQIRQIAERMRGLRDALDLTSSQVAQACELSLEEYEKYESGQ
                     ADIPMSVLSKMSAAFNVEISALITGDEPYMSSYFLTRKDKGKTVERRKAYKYQALAMG
                     FKNAKAEPFIVTVEPNELDKSQLHLNSHHGQEFNMVLEGDLLLIIDGKELILHEGDSI
                     YFDSGKPHAMKALNNRPVRFFAIIL"
     gene            complement(1052..1636)
                     /locus_tag="PKY68_07505"
     CDS             complement(1052..1636)
                     /locus_tag="PKY68_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOH24913.1"
                     /translation="MGTIEVFLIALGLAMDCFAVSIACGLVMNKFRFAPVFSIAFLFG
                     LFQALMPLIGWLMGKQFQEYICHLDHWIAFVLLTFLGGRMIYADLCKRKNRTACKSIN
                     PYKLKVVLGLAVATSIDALAVGLSFSLLKISLWQTVLIIGVVSFALSWLGILLALRYG
                     CRFKIKAELIGGIILIAIGSKILIEHLLTHSMQV"
     gene            complement(1623..2099)
                     /locus_tag="PKY68_07510"
     CDS             complement(1623..2099)
                     /locus_tag="PKY68_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24914.1"
                     /translation="MITTLNKHNKAEYPPMHTAEHILNQTMVRMFGCPRSVNAHIERK
                     KSKCDYLLPRAPSPEEVTEIELRVNQIIGLNLPVHIEIVSLEAASKLADLSKLPEDAS
                     SNIRLVRVGDYDTCACIGDHVENTSQIGRFKIISHDYNEGRWRLRFRLENSPYGND"
     gene            complement(2096..2743)
                     /locus_tag="PKY68_07515"
     CDS             complement(2096..2743)
                     /locus_tag="PKY68_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOH24915.1"
                     /translation="MTKDRYVIFDFDGTLVNTTDLALSIYNRVATTYRCKPIPPEDRH
                     LLFSTKLKELMDRFKVNSFKLFLLQFRIRKELRKHIEDFEPVPHIEAALTQLKEAGYK
                     CGILTSNAKKNVEHFLDIKGLSGLFDFVYQSKHFFGKDLALHQLIKLEKLKKEQVVYV
                     GDEIRDLVASQKAGIPMVAVGWGFTPPSVFEKQTADKLAYSPAELKLCIDELFRL"
     gene            2884..>3497
                     /gene="panB"
                     /locus_tag="PKY68_07520"
     CDS             2884..>3497
                     /gene="panB"
                     /locus_tag="PKY68_07520"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOH24916.1"
                     /translation="MSVHNEDSRKVTTHRLMEMKQRGEKIAMLTAYDYSMAKLIDQAG
                     MDVILVGDSASNVMAGNVTTLPITLDQMIYHGKSVTKATKRALVVVDMPFGSYQGNSM
                     EAISSAIRIMKETHADALKLEGGSEIIESVQRILSAGIPIMGHLGLTPQSINKFGTYT
                     VRAREESEAKKLIEDARLLQEAGCFGIVLEKIPAMLAQLITEEL"
BASE COUNT          931 a          803 c          820 g          943 t
ORIGIN      
        1 ctcttcgcct acggccacaa ccattttgat actggcagcc ttattgcggt aaaccaagtc
       61 ttttggggtc agcaaatgag tggccggcac agcaatggca ccgattttat gcaagcccag
      121 taaacaaaac caaaactcaa ttcttcgctt aagaatcagc atgactttgt cgccatgccc
      181 aatgcccatc tgttgaaaaa aggaggcagc ctggtcggag cgaatcttga tttcggcaaa
      241 agtataatcg tgatgcaagc cctggtcgtt ggtccagcaa acagctcttt tgtcgggttg
      301 ctcacgggcc cagacgtcag ctacatcgta ggcaaaattg aaggaatcgg gtacgtttat
      361 atggaaattc cggcggaaat cgtctaagga atcaaattca actttggaca aaaatttttc
      421 taacatattt atagaatgat ggcaaagaaa cgaacgggac gattattcag ggctttcatg
      481 gcatgaggct tgcccgaatc gaaatagatg ctgtctcctt cgtgcagaat cagctccttg
      541 ccgtcgatga ttaatagtaa atcgccttcg agtaccatat tgaactcctg gccatggtgg
      601 ctgttcagat gcaattggga tttatccaac tcgttgggct cgaccgtgac aataaaaggc
      661 tcggctttag cgtttttgaa acccatggcc aaggcctggt atttgtaggc ttttcgtcgc
      721 tcaacggtct ttcctttgtc tttgcgggtg agaaaataag agctcatata gggttcatcg
      781 cccgtaatca gagctgagat ctccacattg aaagcagctg acattttact taatacactc
      841 atggggatgt cggcctggcc gctttcgtat ttttcgtatt cctccaggct gagttcgcat
      901 gcctgggcaa cctggctgct tgtcaggtcg agtgcatcgc gcaaaccccg cattcgttcg
      961 gcaatttgcc tgatttgttc attcataagt ccggataaat tgattattac ttttcagagc
     1021 gtgtctgagc cgcaaatgta aggaattttt gctacacctg catcgaatgc gtgagcaaat
     1081 gttcaatcaa aatcttgctt cctatggcta tcagaatgat tcctcctatc agttcagcct
     1141 ttatcttaaa ccggcagccg tatcgcagag ccagcaaaat gcccagccag gataaagcaa
     1201 aagaaaccac gccgattatc agcacggttt gccacaaact gatttttaac agggaaaacg
     1261 ataaacctac tgctaaagca tcgatgctgg tggccaccgc caggccgagc accactttaa
     1321 gtttataggg attgatggat ttgcatgccg ttcgattttt tctcttgcac aaatcggcat
     1381 agatcattcg tccccccaaa aacgtcagta agacaaaagc gatccagtga tccagatggc
     1441 agatgtattc ctgaaactgc ttgcccataa gccagccaat caggggcatc agggcttgaa
     1501 aaagaccgaa aaggaaggca atgctgaaga ccggtgcaaa acggaattta ttcattacca
     1561 agccacaggc tatggatacg gcaaaacaat ccatggccag gcccagggct atcaaaaaga
     1621 cttcaatcgt tcccatacgg agagttttcc aaacggaatc tcagccgcca gcgaccctcg
     1681 ttgtagtcgt ggctgattat tttgaaccgg cctatttgcg aggtgttttc cacgtgatct
     1741 cctatgcagg cacaggtgtc gtaatcaccc acccgaacca accggatgtt ggaagaggca
     1801 tcttcgggca atttgctcaa atcggccagt tttgaggctg cttcgaggct gactatttct
     1861 atatggacgg gaaggttcaa accgatgatt tggttgactc tgagctctat ttctgtcact
     1921 tcctcgggcg agggagccct tggaagcaag tagtcgcatt ttgatttttt gcgctctata
     1981 tgtgcattca ccgagcgggg gcagccaaac atccgcacca tggtttggtt taaaatatgt
     2041 tcagccgtgt gcatgggcgg atattctgcc ttgttgtgct tgttgagtgt ggtaatcata
     2101 gccggaaaag ttcgtcgata caaagtttga gttcagcagg tgaataagcc agtttgtcgg
     2161 cggtttgctt ttcgaaaacg gagggagggg taaagcccca gcctacggcc accataggaa
     2221 taccggcttt ttgtgaagcc accaaatcgc ggatttcgtc gcccacgtaa accacttgtt
     2281 ccttttttaa tttttcgagt ttgatcaatt gatgcagggc caaatctttg ccaaaaaaat
     2341 gtttgctttg gtacacaaaa tcgaataagc ccgacaggcc tttgatatcc agaaaatgtt
     2401 ctacgttctt ttttgcgttc gaggtgagta ttccgcattt gtagccggcc tctttgagtt
     2461 gggtcaaagc tgcttctata tggggtacag gttcaaaatc ttcgatgtgt ttgcgcaatt
     2521 ccttccggat gcggaattgc aataaaaata atttaaagga atttaccttg aaacgatcca
     2581 tgagctcttt gagcttggtc gaaaataaca aatgccggtc ttcgggagga atgggcttgc
     2641 agcggtaagt tgtcgccact ctgttgtaga tgctcagagc caaatcggtt gtattgacca
     2701 gcgtgccgtc aaaatcgaag atcacgtagc ggtcttttgt catatcctgt acttttcttg
     2761 caataattgc gcaaaagtaa acattttacc cagaatcgta aagttgataa gggtcggaaa
     2821 ttttgcggca ttattaaatt taagctttat ctttgtcggt ttaattcaaa atacatagct
     2881 atcatgtcag tacataatga agattcccgc aaagtcacga cgcatcgtct gatggaaatg
     2941 aaacagcggg gcgaaaaaat tgccatgctc acagcctacg attattccat ggcaaaattg
     3001 atagatcagg ccggtatgga tgtcatactc gtcggagatt ccgcttctaa cgtaatggcc
     3061 ggcaatgtta ccactctgcc catcaccctg gatcagatga tttaccacgg caaatccgtg
     3121 actaaagcca ccaaacgggc tttggtggtg gtagacatgc cttttggaag ctatcagggc
     3181 aattccatgg aagccatctc gagcgccata cgcataatga aagaaaccca cgccgacgct
     3241 ctcaaactcg aaggcggttc cgaaattatc gaatcggtgc aacgtattct cagcgccggc
     3301 attcccatca tgggtcattt ggggctgaca cctcaatcta tcaataagtt tggcacttat
     3361 acggtgcgtg ccagagaaga atccgaagcc aaaaaactca tcgaagatgc ccgtttgttg
     3421 caagaagccg gctgttttgg tattgtgctg gaaaaaattc ctgccatgct cgctcaatta
     3481 attaccgagg agctcaa
//
LOCUS       DAOSGL010000247         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_25238_length_3469_cov_3.747217, whole genome shotgun sequence.
ACCESSION   DAOSGL010000247 DAOSGL010000000
VERSION     DAOSGL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25238_length_3469_cov_3.747217"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..881)
                     /gene="surE"
                     /locus_tag="PKY68_07525"
     CDS             complement(120..881)
                     /gene="surE"
                     /locus_tag="PKY68_07525"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOH24917.1"
                     /translation="MKPLFLISNDDGIHARGIRCLIDVLRPLGDMLVVAPETERSGMS
                     TAFTLREPLTCKPVSKEEGLIQYSSNGTPVDCIKLALNLLTDERKPDLIIAGINHGFN
                     GSIAIHYSGTMGAVLEGCMAGYPSIGFSIDDHREEANFEPSLPHIRNIVQAVLDNGLP
                     YGVCLNVNIPKHDEIKGTKICRQGKGRWVEEFIPCSHPDNAKRYWLTGKFLNLEENAG
                     DNDLTSMRQGYISVVPSCIDVTNHSLIGQLQTWGL"
     gene            1088..1543
                     /gene="rplM"
                     /locus_tag="PKY68_07530"
     CDS             1088..1543
                     /gene="rplM"
                     /locus_tag="PKY68_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOH24918.1"
                     /translation="MDSLSYKTISVNKATAQKEWVVVDATDQVLGRLGTKVAKLLRGK
                     YKPSFTPHVDCGDNVIIINADKVRLTGSKWNDRIYLRHTGYPSGQRKFTPASLMAKDP
                     LSLYRKVVRGMLPKNRLGEAIINNLYVYVGNEHPHQAQKPKAIDLNSLK"
     gene            1547..1933
                     /gene="rpsI"
                     /locus_tag="PKY68_07535"
     CDS             1547..1933
                     /gene="rpsI"
                     /locus_tag="PKY68_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOH24919.1"
                     /translation="MEAVNAIGRRKAAIARVFVTEGNGTININKKDLSEYFPSDILQY
                     VVKQPLKKLEVDGKYDINVSLKGGGFKGQAEALRLAISRALVKINPEDKPALKAEGFM
                     TRDSRIVERKKPGRPKARKKFQFSKR"
     gene            2069..2935
                     /gene="rpsB"
                     /locus_tag="PKY68_07540"
     CDS             2069..2935
                     /gene="rpsB"
                     /locus_tag="PKY68_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOH24920.1"
                     /translation="MSRTNFDQLLEAGVHFGHLKRKWNPAMAPYIFMERNGIHIIDLH
                     KTITKLDEAAEAMKNFARSGKKILFVATKKQAKEIVAERAASVNMPYVIERWPGGMLT
                     NFPTIRKAVKKMSTIDKMFTDGTYSNLSKRERLQIGRKRAKLEKNLGSIADLNRLPSA
                     LFVVDVMKEQIAVHEANRLGIPVFAMVDTNSDPSNIDFVIPANDDATKSIDIIVSTVC
                     AAIAEGLEERKIEKADADAAAAVAEEEEGNENVSRRERRPKTARRERIQKEDEEALKA
                     RVTSKFMKDDDE"
     gene            2994..>3469
                     /gene="tsf"
                     /locus_tag="PKY68_07545"
     CDS             2994..>3469
                     /gene="tsf"
                     /locus_tag="PKY68_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOH24921.1"
                     /translation="MAVTMADISKLRSMTGAGMMDCKNALTEANNDMEKAIEIIRKRG
                     QAIAAKRSDREASEGCVLADCKDGFAAIVALKCETDFVAKNQDFIALTKSILDLALAT
                     KPESLEALQNSTMDGRTVADLVTERSGITGEKMELGYYECVKAASVMHYVHPGNKLA"
BASE COUNT         1040 a          736 c          855 g          838 t
ORIGIN      
        1 acgaaattcg gccttggggt ctttttcgac cagggctgcc atcaggttgg cggcgtgcat
       61 gtcgcccgag gcttcaccgg caattagata gtacttcatc agttcaaagg ctcaataact
      121 caaagtcccc aggtttgcag ttgaccgata agagaatggt tggttacgtc gatgcatgag
      181 ggcaccaccg aaatataacc ctggcgcatg gatgtcaggt cgttgtcgcc ggcattttct
      241 tcgaggttga gaaatttgcc ggtcagccag taacgtttgg cattgtcggg atgcgaacag
      301 gggataaatt cttcgaccca gcgtcccttg ccctggcggc atatcttggt tcctttgatt
      361 tcgtcgtgct ttggaatgtt cacgttgagg cagacgccgt aaggcaggcc attgtcgagc
      421 actgcctgca caatgttgcg aatgtggggc agggaaggct caaaattggc ttcctctctg
      481 tggtcgtcta tggaaaagcc tatcgatggg tagccggcca tgcagccttc gagtacggct
      541 cccatggtcc cggaataatg gatggctatg gagccgttga aaccgtggtt gatgccggca
      601 atgatcagat cgggtttacg ctcgtcggtc agcagattca gtgccagttt gatgcaatct
      661 accggtgtgc cgttgctcga atattggatc aggccttctt cttttgatac cggtttgcag
      721 gtgagaggct ctcttagggt aaaagccgta gacataccgg atcgttcggt ttcgggggcc
      781 actaccaaca tatctcccaa aggtctcaac acatcaataa ggcagcgtat tccacgagcg
      841 tgaataccat catcgttcga aatcaaaaac aaaggtttca taggcgtaat tcaaaataac
      901 aggcgcaaag gtagtcaaaa aaaaacgtag attcttttgt gagatacgaa aattcgccgt
      961 acttttgcag gccaattctc tatggttttc taataagatt ttagttcata aggaattaag
     1021 ctcgaaagcc ttgagatttg tttattttat acgataatta attaacaatt aaaatccaaa
     1081 aaaagcagtg gacagtttga gttacaagac catttctgtt aacaaggcaa cggctcaaaa
     1141 agaatgggtt gtagttgatg caaccgatca agtattggga cgtttgggga cgaaggttgc
     1201 caagttgctg agaggtaagt acaaaccaag ctttacaccc catgtggatt gtggtgacaa
     1261 tgtcatcatt atcaatgcgg ataaagtcag gcttaccggt tcgaaatgga atgatcgcat
     1321 ttatttgcgc cacacgggat atccgagtgg gcaaaggaaa tttactccgg cttcgttaat
     1381 ggccaaggat ccattaagtc tataccggaa agtggtaagg ggtatgcttc ccaaaaaccg
     1441 tttgggtgaa gctattatca acaatttgta tgtgtatgta ggaaacgaac atcctcatca
     1501 ggcacaaaag ccgaaggcaa tcgatttaaa ctcacttaaa taaattatgg aagcagtaaa
     1561 tgcaatagga agaagaaagg cagcgattgc ccgtgtattt gttactgaag gaaacggaac
     1621 cattaatatc aacaaaaagg atctgtcgga gtattttcct tccgatattc ttcagtatgt
     1681 ggttaaacag ccgctaaaaa aactggaagt tgacgggaaa tacgatatca acgtaagcct
     1741 taaaggaggc ggtttcaaag gacaagccga ggctttgcgc ctggccattt cccgcgccct
     1801 tgttaaaatc aatcccgaag ataagcctgc tttgaaggcc gaaggattta tgacccgcga
     1861 ttcccgcatt gtggaacgca aaaaaccagg tcgtcccaaa gcaaggaaga aattccagtt
     1921 cagcaagcgt taatattttg ataccactgc tcgataaatg aaaaagtagc gtttagtatc
     1981 taaatggtca ggacttccgg ctcaggccga gaactaccgg acgattgaat gagccccaaa
     2041 agaatgtaaa cgaaattaat taaccatcat gtcaagaact aattttgatc aattactgga
     2101 agccggagtg catttcggac accttaaaag aaaatggaac cccgccatgg ctccgtacat
     2161 atttatggaa cgtaacggca tccacattat agacctgcac aaaacaatta ccaaactgga
     2221 cgaggctgcc gaggcgatga aaaattttgc ccgttcggga aagaaaatac tctttgttgc
     2281 caccaaaaaa caggccaaag aaatcgttgc tgaaagggct gcatccgtca atatgcccta
     2341 tgttatagag cgttggcccg gaggaatgct caccaatttc cccaccatcc gcaaagctgt
     2401 aaagaaaatg tctaccatcg acaaaatgtt tacagacggc acgtatagca atttgtccaa
     2461 aagggaacgt ttacagattg gtcgcaaaag agccaagttg gaaaagaacc tgggcagtat
     2521 tgccgatttg aacagattgc cttcggcttt gtttgtggtc gatgttatga aagaacaaat
     2581 tgccgttcac gaggctaatc gtctgggtat tcccgttttt gccatggtcg ataccaattc
     2641 cgatccttcc aacatcgatt tcgttattcc cgccaacgac gacgccacca aatctataga
     2701 tattatagta tcgacggttt gtgccgccat tgccgaaggt ctggaagaac gcaagattga
     2761 aaaagccgat gccgacgctg ctgctgccgt tgccgaagaa gaagagggta acgaaaacgt
     2821 aagccgcaga gagcgccgtc caaagacagc acgcagggaa cgtattcaaa aagaagacga
     2881 ggaagcactg aaagcaagag taacctcgaa gtttatgaaa gacgacgacg aatagtcgga
     2941 acatgccgga atggaaccgg tgacgaagat tgtttacaaa aaaaagaaga attatggcag
     3001 taactatggc agatatctcg aaactccgtt ctatgaccgg ggccgggatg atggattgta
     3061 agaatgcgct taccgaagcg aacaacgata tggaaaaagc gatagaaatc attcgcaaaa
     3121 gaggtcaggc aattgctgca aaacgcagtg acagggaagc ttccgaagga tgcgtattag
     3181 cggactgcaa agatggattt gccgccatcg tagccctgaa atgtgaaacc gattttgtgg
     3241 ccaaaaacca ggattttatt gctcttacca aatctatcct cgatttagct ctggcaacaa
     3301 agcccgaaag cctcgaagct ctgcaaaaca gcactatgga tggcaggaca gtggccgatc
     3361 tggttaccga acgcagcggt attacaggcg aaaaaatgga attgggttat tatgaatgtg
     3421 tgaaagctgc ttctgtgatg cattacgtgc atcccggaaa taagctggc
//
LOCUS       DAOSGL010000248         3465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_25284_length_3465_cov_4.195308, whole genome shotgun sequence.
ACCESSION   DAOSGL010000248 DAOSGL010000000
VERSION     DAOSGL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25284_length_3465_cov_4.195308"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKY68_07550"
     CDS             <1..217
                     /locus_tag="PKY68_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24922.1"
                     /translation="YTLPKRWTEKIKIEKLRIYAVGDNLALFSARKGFDPRTMIGAGV
                     GSLGTAGTGSHTYTALRTISGGISLTF"
     gene            245..1882
                     /locus_tag="PKY68_07555"
     CDS             245..1882
                     /locus_tag="PKY68_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24923.1"
                     /translation="MKKRNIFFLALGLVLLGSCTDLNTIPEGGTLTEEQKREVVEAIP
                     GRLAADINGMFAVLSKQYCVFGAASGRDDDHGYPAAAMSWDLNGPDMVGTDDNYNWFS
                     VASDYADRTYTYANPYMRWAMFYKQIKMANDVMASIPEDTDNPTLLTYRGLAHAVRAF
                     DYFSLVQYYQFTYGPETLDKPAVPIVEPGMTDVTNPRATVRQVYELIMNDIDTAIVLL
                     KDYVPTSKSYISEAVAYGLRARVNLVMKNYAQAALDARKAIDLFGGTPCTREELMTPG
                     FIDATDHNWMWALIITPDVVPDAYPAWPAVLASFSGDSYTCGVGCYKMINTLLYSIIP
                     ATDVRKGWWVDSNLKSPLIDSLSWPEYKGEPLGPLNIPDVKMPYLPYTNVKFGQFGGI
                     GNTVNAGDWCMMRVEEMLLIEAEATGMTDEAAGKNLLTNFVKTYRDPAYSFPASVKFQ
                     DECWKQRRIELWGEGFSMMDIMRLKKNIVRFNSRVETNHPEAFRFNLAADDGWLLMRI
                     PQREINSNPGISDEDNNNDGALPVSGDGAGLRDGVTD"
     gene            1916..2827
                     /locus_tag="PKY68_07560"
     CDS             1916..2827
                     /locus_tag="PKY68_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24924.1"
                     /translation="MKNFKRIFFIIGLGAFLWSSCTEDIPVREESPAADPESMQVYFP
                     NSQKYKYELPPTVFEVDIDLKREKSAKAATVKIIVTSDLTGVFTVPETVSFAAGDSTA
                     KLTITFDTLEQFKPYQISFKIEDQYINPYVVNPDGTTDFNLYVEHNDFLPVGNGKGQF
                     FDSFTLASVADVSITYSEKKDVYRISNPYTLALLEEAEWGSWIGGPISENIEIQITSE
                     GKVTWEFWYLGLNYQGVSGYPIKAYFPSVLDESLAALDDKSVKLQDKLFKLHPYFYID
                     GLGGFGADYPVFISLPGGPNLNELLAE"
     gene            3191..>3465
                     /locus_tag="PKY68_07565"
     CDS             3191..>3465
                     /locus_tag="PKY68_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24925.1"
                     /translation="MAYPYPIEIDLPDGSKLEIQRHGDEFFNYTTDQNGYIIAQKPDG
                     YYYYANYDEQAKLNISNYRAGSRAARCSAVVPEVNFRRAAQTRSERY"
BASE COUNT          939 a          789 c          806 g          931 t
ORIGIN      
        1 ttatacctta cctaaacgtt ggacggaaaa gatcaaaatt gaaaaattgc gtatatatgc
       61 cgtaggcgat aatctggcct tgttctcggc tcgtaagggt tttgatccca gaaccatgat
      121 cggtgccggc gtaggttcgc ttggtacggc aggaacaggt tctcatactt acactgcact
      181 ccggaccatt tccggaggca tatcccttac attttaatac gtcacattat aaaactgtat
      241 aaagatgaaa aaacgtaata ttttcttttt agcgctcgga ttggtgttgc tgggatcttg
      301 tacggatctg aatactattc ctgagggagg tactcttacc gaagaacaga aaagggaggt
      361 ggtcgaagct attcccggcc gcctggcagc agacatcaat ggtatgtttg cggtcctttc
      421 taaacaatat tgtgtatttg gagcagctag tggacgtgat gatgaccacg gctatcctgc
      481 ggcagccatg tcctgggatc tgaatggccc cgatatggta ggtacagacg ataattacaa
      541 ctggttctcc gttgcttccg attatgccga caggacctat acttatgcca atccctatat
      601 gaggtgggcc atgttctata agcagatcaa aatggcaaac gacgtaatgg cgtctattcc
      661 agaagatacc gataatccga ctttgttgac ctacagaggt cttgctcatg ctgttcgtgc
      721 ctttgattat ttctcgttgg ttcaatacta ccaatttacc tatggtccgg aaactttgga
      781 taaaccggct gttcctattg tagagcccgg aatgaccgat gtaaccaatc ccagagcaac
      841 cgtacgacaa gtgtacgaat tgattatgaa cgacatcgac acggccattg ttttactcaa
      901 ggattacgtg ccgacatcca agtcttacat cagcgaagcc gtagcctatg gtcttcgtgc
      961 cagagtcaat ctcgtaatga aaaactacgc tcaggctgca ttggatgccc gtaaggccat
     1021 cgatttattc ggaggaactc cttgtaccag agaggaactt atgactcccg gatttataga
     1081 cgcaaccgac cacaattgga tgtgggcttt aatcattact cccgacgtgg tgcctgatgc
     1141 ttatcctgcg tggcccgccg tacttgcttc cttctcgggc gacagttata cctgcggggt
     1201 gggttgttac aagatgatta ataccctgtt gtattcgatt attcctgcca ccgatgtacg
     1261 taaaggctgg tgggttgaca gcaatttaaa atcacctctc atcgacagtc tgtcatggcc
     1321 cgaatacaaa ggcgaacctc taggtccgtt gaacattccc gatgtaaaaa tgccctattt
     1381 gccttatacc aatgttaagt ttggccaatt tggtggaatt ggaaacacag taaatgcggg
     1441 cgactggtgt atgatgcgtg tagaggaaat gctcttgatt gaagctgaag ctacaggtat
     1501 gactgacgag gccgccggaa agaatttgtt gacaaatttt gtgaaaacct acagagaccc
     1561 cgcttacagc ttccccgcct ccgttaaatt ccaggacgaa tgctggaagc agcgccgtat
     1621 agaactgtgg ggcgaaggtt tctccatgat ggacattatg cgtctcaaga aaaacattgt
     1681 gcgttttaat tccagggttg aaaccaatca tccggaagct ttccgtttca acctagctgc
     1741 tgacgacggc tggttactga tgcgtattcc ccaaagagaa atcaactcga accccggtat
     1801 cagcgatgaa gacaacaaca acgatggagc cttgcccgta agcggtgacg gtgcaggcct
     1861 tagagatggt gtaacagact aattaaacaa gaatcattta ataatcatgc acgctatgaa
     1921 aaattttaaa agaatattct ttatcatagg gctgggggcc ttcttatggt cgtcctgtac
     1981 cgaggatatt cccgtaaggg aagaatctcc ggcggctgac cccgaaagta tgcaagtata
     2041 cttccccaac agccaaaagt acaaatatga attgcccccg accgttttcg aagtcgatat
     2101 tgatctgaaa cgcgaaaagt ctgcgaaggc tgctacggtt aaaattatag tgacctccga
     2161 tttgaccggc gtttttaccg tacccgaaac tgttagtttt gccgcgggcg attccacggc
     2221 caagcttacc atcaccttcg atactcttga acaatttaag ccctaccaga tatcgttcaa
     2281 gatagaagat caatacatca atccttatgt tgttaacccc gatggtacta cagactttaa
     2341 cctgtatgtt gagcacaacg acttcctgcc tgtgggtaac ggtaagggtc aattcttcga
     2401 cagctttacc ttggcctcag ttgccgatgt atctatcact tactctgaga aaaaggacgt
     2461 ttaccgtatt agtaatccct atactctggc tcttctcgag gaagccgaat ggggaagctg
     2521 gattggaggt cctataagcg agaatataga aatccagata acgagcgaag ggaaagtaac
     2581 ctgggagttc tggtatcttg gattgaatta tcagggtgtc agcggatatc ctataaaagc
     2641 ttatttcccc tcagtccttg atgaatcact agctgctctt gacgacaaga gtgttaagct
     2701 tcaggataag ctgttcaaac tgcatcctta tttctacata gacggacttg gtggttttgg
     2761 cgctgattat ccggtattta tttctttacc cggtggcccc aatctgaatg aacttttggc
     2821 agaataatcc taagtttctg aaaccataga atgaaagccc ttcctgattc aggaggggct
     2881 tttttttaga gcatctttcg gggcaggcat ctttcggggc aggcatcctt cggcaagcat
     2941 ccttcagcgg gcatccttcg gcaagcatcc ttcagcgagc accttcggtg agcatccttc
     3001 agcgggaaag cagtaaattc cgtcgaatac atcaaaaccc cggttaagcg accgggtata
     3061 tctgccaaag gaactcattg gattaaaaat aaatatgtat ttttgtgcgt tacaaaaaaa
     3121 agcaatgcac aggaaacaga tactcatttt tatattctgc ttgctatgtt cgcgggcgct
     3181 ttgggcagtg atggcttatc cctaccctat agaaatcgat ttgcccgatg gcagtaagtt
     3241 ggaaatacaa cgtcatggag atgagttttt taattacact accgaccaaa acggttatat
     3301 tatagctcaa aaaccggacg gctattacta ctacgccaac tacgacgaac aagccaaact
     3361 caatatcagc aattaccgtg ccggcagccg ggcagcaagg tgttcggccg tggtgcctga
     3421 agtcaatttc aggcgggcag ctcagacccg aagcgagcgt tacga
//
LOCUS       DAOSGL010000249         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_25340_length_3460_cov_5.140088, whole genome shotgun sequence.
ACCESSION   DAOSGL010000249 DAOSGL010000000
VERSION     DAOSGL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25340_length_3460_cov_5.140088"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..2605
                     /locus_tag="PKY68_07570"
     CDS             131..2605
                     /locus_tag="PKY68_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOH24926.1"
                     /translation="MHLPVSEIARLIGVQKARIVQDTVIERLLIDSRALLVPEGCLFF
                     AIKTESNNGHKYLSDLYKRGVRNFVLSEKQPESLFDQANFLYVEDAIEALQLLGRYCR
                     KIFEGKVLGITGSNGKTIVKEWIWQLLRDNLSISRSPGSYNSRLGVPLSLSMLSEDSR
                     LGLIEAGISQVGEMQKLQNMIQPDWGLLTHIGQAHNENFDSVESLVREKLLLFKDCKR
                     VIYCQDEEPSAGILAGMLEPKRLLAWSCNDLSAELYFRKDKQKDYTQIHTLWHREEVC
                     FVLPFVDEPSIKNALHCIAFLFLLEQETDFVIDASVLGGRLKQLEPVAMRLEVKEGRN
                     GCVLIDDAYNSDLQALELALDFQSRRALAGKMRSCLILSDIFESGKSMDDLYGTVAEL
                     IKRKQIDRFIGIGYGLVTHAGFFPSSARFFLTTDDFLLSDLPDQFQDELILIKGSRQF
                     GFERISERLELKTHNTVMEVDLNALVHNLNYFRTLIKPQTKVIAMVKAEAYGHGALEV
                     SKTLQAQGCDALAVALADEGVKLRRGGIHIPILVMNPDPGIFPLCYEYKLEPEIYSFA
                     LLDAFMASAERLAVRDFPVHIKLDTGMHRLGFTEKDLPELLSKLNSRPELRVSSVFSH
                     LAGSDENAFDKYTKTQWKLFKLYTRQMSRALPYRFERHILNSAGIERFPQWQCDRVRL
                     GIGLYGVSVCSPEALQPVASLKTRILQIHEFKKGESIGYSRTVLLDRDSRIATLPVGY
                     GDGFNRHHTGGDVLVNGQRAKILGNICMDLCMVDVTDIAAHEGDTVVLFGKGLPLTEL
                     AERLSTISYEVLTSVSERVKRVYFKE"
     gene            2648..>3460
                     /locus_tag="PKY68_07575"
     CDS             2648..>3460
                     /locus_tag="PKY68_07575"
                     /inference="COORDINATES: protein motif:HMM:NF037312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24927.1"
                     /translation="MIRKSLIGWSLVFLACLFASCEGESLFTHNGKEEADSLSLNNSY
                     VNKWIYNGMSLAYYWSDKMIEPEDYTQSPISFFNDLLYRYHPTTAPDGDRFSFIRSDY
                     ANLLNTFSGLRSREIGFDYQLYLRSENDKAIVGQVTYVKPNTPAVDAGVQRGWWFDQI
                     GGIALNTDNYTDLLKNLPAEITLRFLNRAPQQTPENFEAYLNAMHVDTLTFAVLEKYA
                     ENPNYLDTVYQIGNKKIAYFLYNFFAFGPEDKSDRFLLETNEIFGRFKALDVD"
BASE COUNT          929 a          711 c          851 g          969 t
ORIGIN      
        1 cgtcccgacg acgaagagta tcttcgtttt attaagaaaa atatagcagc cctgcatttg
       61 ttgggcaagc gctttcccga actgaatctg gaaaaggaaa gagatttttt tgaatcagta
      121 ttacattcca atgcatctac ctgttagtga aatagcccgc ctaatcgggg ttcaaaaagc
      181 aagaattgtg caggacaccg tcattgaacg tctgctcata gacagcaggg ctttgttggt
      241 gccggagggc tgtttgtttt ttgccattaa aaccgaaagc aacaacgggc ataaatacct
      301 gtccgattta tacaaaaggg gggtaagaaa ttttgtactc agcgaaaaac agccggaatc
      361 gctctttgat caggctaatt ttctctatgt ggaagatgcc atcgaagctc tgcaattatt
      421 gggccgatac tgcaggaaaa tttttgaagg gaaagtgttg ggcataaccg gcagtaatgg
      481 taaaaccata gtgaaagagt ggatttggca actgttgcgc gataatctca gcatcagccg
      541 ttcgccgggc agctacaatt cccgtttggg tgtgcctttg tccctgtcca tgcttagcga
      601 agacagtcgt ttgggcctta tcgaggccgg gatttctcaa gtgggcgaaa tgcaaaagtt
      661 gcaaaacatg attcagcccg attggggttt attgacgcat attggccagg ctcataacga
      721 aaactttgat tccgtcgaga gtttggtacg ggaaaaattg ctcttgttca aagactgtaa
      781 acgggtaatt tactgtcagg acgaagaacc gtcagccggt attctggccg gaatgctgga
      841 acctaaacgt cttttggcat ggtcatgcaa cgacctgagc gcagaactgt attttcgcaa
      901 agacaagcaa aaagactata cccagattca tactttgtgg cacagggaag aagtttgttt
      961 tgttttacct tttgtagatg aaccttctat aaaaaatgcc ctgcattgca ttgctttttt
     1021 gtttctgctt gaacaagaaa cagactttgt aatagatgct tcggtactag gcgggcgttt
     1081 gaagcaactc gaaccggtgg ccatgcgttt ggaagtgaaa gagggcagga atggctgtgt
     1141 tttgatagac gatgcctata actcagattt acaggctctt gagctggcct tggattttca
     1201 atcgaggcgg gctttggccg gaaaaatgcg atcttgttta atcttgtccg acatcttcga
     1261 aagcggtaag tctatggacg atttatacgg tacggtggct gaactgatta agcgaaaaca
     1321 gatagatcgc ttcattggta taggttacgg cttggtaact catgccggat ttttcccctc
     1381 ttccgctcgc ttttttttga ctacggacga ttttttactc tcggatttgc ccgatcaatt
     1441 tcaggatgaa ttgattttga taaaaggttc caggcaattc ggtttcgaac gcatcagcga
     1501 acgcctggaa ttgaaaacac acaacaccgt catggaggtt gacctgaacg ctctggtgca
     1561 taacctgaat tacttcagaa ctctgatcaa gcctcaaacc aaggtaatag ccatggtcaa
     1621 ggcggaagcc tacggacacg gagctcttga ggtaagcaag actttacagg cccagggttg
     1681 tgacgctttg gctgtggctc tggctgatga gggggtgaaa ttgagacgtg gcggtatcca
     1741 tattcctatt ttggtaatga atcccgatcc cggaattttt cctctttgtt acgaatacaa
     1801 actggagccg gagatttaca gtttcgcttt actggatgct tttatggctt cagccgaacg
     1861 tctggcagtc cgtgattttc ccgtgcacat caaactcgat accggtatgc atcgcctggg
     1921 tttcaccgaa aaagacttgc cggaattgtt gtcaaaactc aacagccggc ctgaattgag
     1981 agtcagttct gttttttctc atctggcagg cagtgacgaa aatgcttttg ataagtatac
     2041 aaagactcaa tggaaactgt ttaaacttta cacccggcaa atgagccggg ccttacccta
     2101 tcgttttgaa cgtcatattt tgaattccgc cggtatagaa cgtttccctc aatggcaatg
     2161 cgacagggtg cgtctgggta tagggcttta cggagtgtcg gtttgtagtc ccgaggcctt
     2221 acagccggtt gcttctctca aaaccagaat actgcagatt catgaattta aaaaaggcga
     2281 aagtataggg tacagccgta ccgttttgct cgacagggat tcacgtatag ccactttacc
     2341 ggtaggttac ggcgacggtt tcaaccggca tcataccggt ggagacgtat tggtcaacgg
     2401 acagagagca aaaatattgg gcaatatttg tatggatctg tgtatggtgg atgtgacaga
     2461 tatagctgcc cacgaaggcg atacggtggt gttgtttggt aagggactgc ctttgacgga
     2521 actggcagaa cgcttatcta ccatttcgta cgaagtgctg acttctgtat ccgaaagagt
     2581 caaaagggtg tatttcaaag aatagaccaa attatgtata aagctttatg aaaaaatatt
     2641 tattgagatg ataaggaaat cgcttatagg ctggtccctg gtttttctgg cctgcttgtt
     2701 tgcttcttgt gagggagaaa gcttgtttac acataatgga aaagaagaag cggacagcct
     2761 cagtctgaac aatagctatg tgaataaatg gatctacaac ggtatgtccc tggcgtatta
     2821 ctggagcgat aagatgatag agcccgaaga ttacactcag tcaccaatct ccttttttaa
     2881 tgacttgctt tatcgttacc atcctacgac agctcccgac ggcgaccgtt tttcttttat
     2941 tcgaagcgat tacgccaatt tgctgaatac ctttagcgga cttcgttcac gggaaatagg
     3001 ctttgattat caattgtatt tgcggtcgga aaacgacaag gctatagtgg ggcaggtgac
     3061 ttatgttaaa cccaatacgc ctgccgtgga tgcaggtgta cagagaggct ggtggtttga
     3121 ccagataggg ggaattgcgc tgaataccga caattacaca gatttgctca agaatttgcc
     3181 tgcggaaatc actctccgtt tcctgaaccg ggcaccacaa caaaccccgg agaactttga
     3241 ggcttacctc aatgcgatgc acgtagacac cttgactttt gctgtgctcg aaaaatatgc
     3301 agagaatccc aattacctgg acacggtcta tcaaataggg aacaaaaaga tagcttactt
     3361 tttgtataat ttttttgcct tcggccctga agataagagc gaccgttttt tgttggaaac
     3421 aaacgagatt ttcggtcgtt tcaaagccct tgatgtcgac
//
LOCUS       DAOSGL010000250         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_25441_length_3449_cov_3.505893, whole genome shotgun sequence.
ACCESSION   DAOSGL010000250 DAOSGL010000000
VERSION     DAOSGL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25441_length_3449_cov_3.505893"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PKY68_07580"
     CDS             complement(<1..1251)
                     /locus_tag="PKY68_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24928.1"
                     /translation="MKKIHSKHTVLPIRLPVLVLTVVALIFASGGCRDPYMGQMFELD
                     EGDETKITNIAFMEKYPEDYSLFLDFLKAADYYNALNDASSTVTVFAPDNAAMDSFMK
                     IKDISSFDQLDSAYARQVIQVHMLRGSLNEASFIQYLNEGSIGIPTLFGDYLTLSYGF
                     TNRDIDDVDLANSKPEDTLSIYINNMASVRDIAHQTVNGMVYKLGGVIRPLIETVVEK
                     MHDYGEYELFLEAIEQAGMTGLLNITSDTVQQAMGGYTVNPVNYTVLAVSDETFRSAG
                     IQTMDDLENYLAEKDPAGRSGIARSDSNSVIRRYVSYHILAGANTKSSLTYSLTPNET
                     KVFDTFQAHEIITIQTRGGQTFINRGSRDVCGFVRSDIKASNGYIHKIDGILPVWCPE
                     PSVVVWDFCNSTDIVSIANSYGAAN"
     gene            complement(1273..2814)
                     /locus_tag="PKY68_07585"
     CDS             complement(1273..2814)
                     /locus_tag="PKY68_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH24929.1"
                     /translation="MKKIKHLVAVVLVMALAFTHSACSDWLEVYPQNNQVSDYYWSSK
                     EEVEAMLNGCYHYLREMVTSQLIPLGELRAGQIFSRSGNTNLQEFRVKETDNISNWGP
                     FYEVINVANGVISNAEKVLSRDDTYDKNRLNAHKAEAYFLRALSYFYLVRNWKDVPLV
                     LFPYEDDSQDYHMAQSDEAEVIAQIKKDLLTALESGAAKEYYVKAWENKGRATKWAIY
                     ALLADVCLWSESYSDAEKYCNLILNSNSAYAPSFLATPSHSSWFSIFNPGNSNESILE
                     LQWSEEDEQLNDLPYLFDPSSSRRVYAYSKKMTELFVEEYTHTQQRMAEAVRTMYGGF
                     YCNPPAAYLAAETGFVWKYFGSEVLTEKRTPEHYDPNFILYRTTEVILMKAEALIMQG
                     PERYSEALELIDQVRRRSNLGSTEIDPLNTTELELLECLLNERATELAGEAKAWYDML
                     RFGRRNDYQYKETFITARLLEFNKQASTSWFRSVLLDNNALFLPVWEQELENNPLLVQ
                     NSYYE"
     gene            complement(2828..>3449)
                     /locus_tag="PKY68_07590"
     CDS             complement(2828..>3449)
                     /locus_tag="PKY68_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH24930.1"
                     /translation="DGKPIVMKNGTYTCFPGDAKYQDINHDGVINQSDIVYIGNANPV
                     VTGGGGFTAKFKQLTLTGFLHYRLGQKIVNQARMNSEAMYGTDNQSLSVLRRWRSPGD
                     DTDIPRALWQYGYNYLGSDRFVEDCSFLRLKTISLSYGLPKNFCRKIKVNTINAFVTA
                     YDLLTWTNYTGQDPEVTLPSRVTDIAMDRAQTPPSKRISGGLIINF"
BASE COUNT          835 a          775 c          882 g          957 t
ORIGIN      
        1 gttggcggca ccgtagctgt tggcaatgga aacgatatcg gtgctgttgc aaaaatccca
       61 gactacaaca gagggttccg gacaccaaac aggcaaaata ccgtcgattt tgtggatata
      121 accgttgctc gctttgatgt cggaacggac aaagccacag acatcccggc tgcctctgtt
      181 tataaacgtt tggccgcccc gggtctggat ggtgatgatt tcgtgagctt ggaaggtgtc
      241 gaaaaccttc gtttcgttgg gagttaaact gtaagtgagt gaacttttgg tgttggcccc
      301 tgccaaaata tggtaggaaa catagcgacg gatcacgcta ttgctgtcgc ttcgggcaat
      361 accgctccga ccggcggggt ctttttctgc cagataattt tccagatcat ccatagtttg
      421 gatgcctgct gacctgaacg tctcgtcgga aacggccagt acggtgtagt tgacgggatt
      481 aaccgtatat ccgcccatcg cttgctgtac cgtatcgctg gtaatattca gcaagccggt
      541 catacctgct tgttcaatgg cttcgagaaa aagctcgtat tctccgtaat cgtgcatttt
      601 ttctaccacc gtttcgataa ggggacggat tactcccccc agtttataga ccatgccgtt
      661 aacggtttga tgagcgatat ccctgacaga ggccatattg ttgatgtaaa tcgacaaggt
      721 gtcttcgggt ttgctgttgg ccaggtctac atcatcgatg tcccggttag tgaagccgta
      781 actcaaggtc aggtaatctc cgaaaagggt aggtatgccg atgcttcctt cgtttaggta
      841 ttggataaaa gaagcttcat tgaggctccc ccgcagcatg tggacctgaa tcacctgacg
      901 tgcataagcc gaatcgagtt gatcgaaaga gctgatgtct tttattttca taaaagaatc
      961 catggcggca ttgtcgggag caaaaacggt aaccgttgaa ctggcatcgt taagggcgtt
     1021 gtaataatcg gcagctttca gaaaatccag aaacagtgaa taatcttcgg ggtatttttc
     1081 cataaaagca atgttggtga ttttggtttc gtcgccttcg tccaattcaa acatttgacc
     1141 catataagga tcccggcagc ctcccgacgc gaaaatgagc gcaacgacgg taagaacaag
     1201 caccggcaat cgtattggta acactgtatg tttggaatgt atctttttca taaaccttag
     1261 tccttgaatt cgttattcat aataggaatt ttgcacaaga agaggattgt tttccaattc
     1321 ctgctcccaa acggggagaa agagcgcatt gttgtcgagg agcacagagc ggaaccaact
     1381 ggtactggcc tgtttgttga attccaataa acgggccgtg atgaaggttt ctttgtattg
     1441 gtagtcgttg cggcggccaa aacgcagcat atcgtaccag gctttggctt cgccggccag
     1501 ttcggtagca cgttcgttaa gcaaacattc gagcaattcc aattcggtgg tattgagcgg
     1561 atcaatttcc gtagaaccca ggttggagcg tctgcggacc tgatctatta attcgagagc
     1621 ttccgaataa cgctcaggcc cttgcataat cagcgcttcg gctttcatta atatcacttc
     1681 ggttgtacgg taaagtataa aattgggatc atagtgttcc ggagtgcgtt tttcggtcag
     1741 cacttcggag ccgaaatatt tccagacaaa gcctgtttct gcagccagat aagcagcggg
     1801 aggattgcaa tagaatccgc cgtacatggt gcgcacggcc tctgccatac gttgttgggt
     1861 gtgggtgtat tcctccacaa acagctccgt catttttttg gagtaggcgt acacgcgtct
     1921 ggaactggag gggtcgaaca aataaggcag atcgttgagt tgttcgtctt cttcgctcca
     1981 ttggagctcc aggatagatt cgttgctgtt gccgggatta aaaatggaga accaggagga
     2041 atgcgaggga gtcgcaagga agctgggggc gtaggcagag tttgaattta aaatcaggtt
     2101 gcaatacttt tcggcatctg aatagctttc gctccaaagg cagacgtcgg ccagcaaagc
     2161 atatattgcc catttggtgg cacggccctt gttttcccag gctttgacgt aatattcttt
     2221 ggctgctccc gattccaaag cagtcagcag gtctttcttt atttgagcaa tcacttcggc
     2281 ttcgtcagac tgcgccatgt gataatcctg agagtcgtct tcgtaaggga acagcaccag
     2341 cggcacgtct ttccagtttc tgaccaggta gaaataactc aaagccctaa ggaaataagc
     2401 ttctgctttg tgtgcattca gcctgttttt gtcgtaagtg tcgtctctgc tcaatacttt
     2461 ttcggcattc gaaataacac cgttggctac gtttatgact tcgtaaaagg ggccccaatt
     2521 gctgatgtta tccgtttctt tgacacgaaa ttcctgcagg ttggtgttac cgctgcggct
     2581 aaagatttga ccggccctga gttcgcccag aggaatcagt tgggaagtaa ccatttcgcg
     2641 gaggtagtgg taacaaccat tgagcatggc ttccacttct tctttgctgg accagtaata
     2701 gtcgcttacc tggttgttct gcggataaac ttcgagccaa tcggagcaag cggaatgggt
     2761 aaaggccaat gccatgacaa ggacgacggc gacgagatgt tttatttttt tcataatgag
     2821 tcctccttta gaaattgatg attaaaccac ccgaaatcct cttgctggga ggggtctgag
     2881 ccctgtccat agcaatatcg gttactctcg agggcagagt tacctccggg tcttgtccgg
     2941 tataattggt ccaagtcaga aggtcgtaag ccgtaacaaa agcgttgatg gtgttgactt
     3001 tgattttccg gcagaaattt ttcggcaaac cgtaagagag agagatggtt tttaacctca
     3061 gaaaagagca atcctcgaca aaacggtcgc tgcccaggta gttgtaaccg tattgccaga
     3121 gagctctcgg tatatctgtg tcgtcgcccg gactgcgcca gcgccttaat accgacaggc
     3181 tctgattgtc ggtgccgtac atggcctcac tgttcatacg tgcctgattg acaatttttt
     3241 gtcccaaacg ataatgtaaa aagccggtta aggtcagttg tttgaatttg gcagtgaatc
     3301 ctccgccgcc ggtgaccacc ggattggcgt tgcctatgta cacaatatcg ctttgattga
     3361 taacgccgtc gtgattgatg tcctggtatt tggcatctcc cgggaaacag gtataggtac
     3421 cgtttttcat tactatgggt ttgccgtcg
//
LOCUS       DAOSGL010000251         3392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_26049_length_3392_cov_3.833683, whole genome shotgun sequence.
ACCESSION   DAOSGL010000251 DAOSGL010000000
VERSION     DAOSGL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26049_length_3392_cov_3.833683"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1710
                     /locus_tag="PKY68_07595"
     CDS             40..1710
                     /locus_tag="PKY68_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24931.1"
                     /translation="MYHKLKLLIVALLFASCTEFFNTNPDNIINVEDYVSTNDEMYKG
                     FLGILTRMQRAGDHAIFLTDTRGDFLEITPNAPVELQDIYNYNPTDGNPYADPTCYYA
                     VVIACNDYLHKMAQYREKVGNSMDEKTQTNFNCLISSTIRIKVWAYYTLGRIYGKAVW
                     FDDPLEELVNLDNTEIFTWYDNMDDLVAKCIELIENGLVIYQGEVIPSDLEMNWVAWL
                     DAENQNVDEYGYWNYLVPTHLLLKSELLSWRGQEGDWLWIRDNILAHLYDLHMEFSGF
                     YHACNIPLTGNYYSIFYTEDVGYSYHQVAAIMYDYENDQTNRLVDYFCPAYPASYYLR
                     PSAYALSKYIEEDIRGITQRLTVDVINGDTCFTKYYYHRGAYLRSRIFEIMPSIVLQR
                     GHDYHFLLAEAENHLGNWMQAECILNQGLTNKFPYSTSLPADWSSYYTSWFGDNGGYG
                     DVGIVGCVRGANHVLPMPDDPGYNLSEQERIKIYDLALADEYLLEYTGEGKSYSYLVK
                     MAERYGKDGSIVADRICPKYPLARQAEIRAGIEAGGYWVDWDLRGDNE"
     gene            1829..>3392
                     /locus_tag="PKY68_07600"
     CDS             1829..>3392
                     /locus_tag="PKY68_07600"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24932.1"
                     /translation="MARNTVVSSSAEFGNAWGALTDGDTISFAPGLMNIGNRTLPATG
                     GTFTFRSQYDHPDSMAIVQLQISGVNTEEGITTGLIFENLHLQYRSPSSTSGQIIYFN
                     KVYADISIVAFRNCEISQSVRSLIRTVKPSDIPATDSTEAVEYTSSGDLEYLEMTDCI
                     VHNTFASSSHNWPLLYFGHLPIEVYIARNTFYDMPYNKSIFTMNYAVPELGRNAEITF
                     ENNTVCITGPTQGIVVTGTYLGQESEFNFHNNLFLVPYWENDLNLHDSLYSAPRIISC
                     RYGIINASHNLVEGYARWESGQNIDAETGDGAFLALDTMPQYTMADLELSWSDFLNPE
                     GGDYSYMYNIPLATAGSDGGPIGDPRWVMTFADPKNLTLVSNAEGAVVTPAMGVYESG
                     TEVTISASEVVGFTFQNWQDTLGNVVSAENPYTFNITSDTYLVAYYEELLSRAITIRL
                     NGTNTATYSLSPAREIYYVGDEVTITVNAHYLNDFLGWSDGSKELSRSFVVANDTTIV
                     ANFRQYPYMLAWD"
BASE COUNT          921 a          757 c          789 g          925 t
ORIGIN      
        1 tagtgttaag ctgggattta atttaaattt ctaagaacca tgtatcataa acttaaacta
       61 cttattgtcg ctttactctt tgcttcgtgt acggaatttt tcaataccaa tcccgacaat
      121 atcatcaatg tggaggatta tgtttcgacc aacgacgaaa tgtacaaggg ttttttggga
      181 attttgacca gaatgcagag ggcaggagat cacgccatct ttttgaccga tacccgaggc
      241 gattttttgg agataactcc caacgctcct gtagaattgc aggatattta taattacaat
      301 cctacggatg gaaatcccta tgccgatccc acctgctatt atgctgtagt tatagcctgc
      361 aacgattacc tgcacaaaat ggcccaatac cgcgaaaagg tgggcaacag catggacgaa
      421 aaaacacaaa ccaattttaa ttgtttaatc agcagcacca ttcgtataaa ggtgtgggct
      481 tattatactt tgggacgcat ttacggcaag gctgtctggt tcgatgatcc tctggaggag
      541 ctggtgaatc tggacaatac ggaaattttt acctggtacg ataacatgga cgacttggtt
      601 gctaaatgca tcgaacttat tgaaaacggt ttggtgattt atcagggaga agttattcct
      661 tccgatttgg aaatgaattg ggtggcctgg ctggacgccg aaaatcagaa cgtggacgaa
      721 tacggatatt ggaattacct ggttccgact catttgttgc ttaaatctga attgctgtcg
      781 tggagaggac aagagggaga ctggctctgg ataagggaca atattctggc ccatttatac
      841 gatcttcata tggaattttc cggtttttac catgcctgca atatcccgct gacggggaat
      901 tattacagca ttttttatac ggaagatgtc ggttacagtt atcatcaggt agctgccatt
      961 atgtacgatt acgaaaacga tcagaccaat cgtttggtag attatttctg tccggcttat
     1021 ccggccagct attatttacg tccctcggct tacgccctga gtaaatacat cgaagaagac
     1081 atccggggta taactcaaag gcttacagtg gacgtaatca acggggatac ctgtttcacc
     1141 aaatactatt atcaccgggg agcctacctg aggtccagga tttttgaaat tatgcccagc
     1201 atcgtgctgc agcggggtca cgattatcat tttttattgg ctgaggccga aaatcacttg
     1261 ggcaactgga tgcaggccga atgtattttg aaccagggtt taaccaataa gttcccttat
     1321 tcgacttctt tgcctgccga ctggagcagt tattatacct cgtggttcgg cgataacggg
     1381 gggtacggcg atgtgggtat agtcggatgc gtgcgcggag ccaaccatgt gttgcccatg
     1441 cccgacgatc ccggttataa tttgagcgaa caagaaagaa tcaaaatata cgacttggcg
     1501 ctggccgatg agtatttgct ggaatacacc ggcgaaggta aatcttacag ttatctggtg
     1561 aagatggccg aacgctatgg aaaagacggg tccattgtgg ccgaccgtat ttgtcccaaa
     1621 tatccgcttg ccaggcaggc agaaatacgg gccggtatcg aagccggagg ttattgggtc
     1681 gactgggact tgcggggaga taatgagtaa taaccactaa attgacaaaa catctaaaca
     1741 ttaatttttc taatctctaa aaccaatttt tatgaaaaaa cgatttactt tatttatgct
     1801 cgttgcattt tgtatttcgc ttgcaacaat ggcaagaaat actgttgtta gcagttcagc
     1861 ggaatttggc aacgcatggg gagctttaac agacggagac accatctctt ttgctcccgg
     1921 tttaatgaac ataggaaaca gaaccttacc tgcaaccgga gggaccttta cattcaggtc
     1981 tcaatacgac caccccgatt cgatggctat tgtacagctc caaatatcgg gggtaaacac
     2041 cgaggaaggg atcactacgg gtttaatttt tgaaaatcta catctgcaat acaggagtcc
     2101 cagctctact tcgggacaga tcatatattt caataaagta tatgccgaca ttagtattgt
     2161 ggcttttcgt aattgcgaaa tttctcaaag cgttcgtagt ttgatccgta cggttaaacc
     2221 tagcgatatt ccggcaacag atagtaccga ggctgtggaa tataccagca gcggcgattt
     2281 ggaatacctc gaaatgaccg attgtatagt gcacaacaca tttgcctcta gtagccacaa
     2341 ttggcctttg ttgtatttcg gacaccttcc tatcgaggta tatatagctc gtaacacctt
     2401 ttacgacatg ccctacaaca agagtatttt cacaatgaac tacgccgttc ccgaactggg
     2461 caggaatgct gaaataacct ttgaaaacaa tacggtttgt ataaccggtc caactcaggg
     2521 gatagtggtc accggcacct atttaggaca ggaatcggag tttaattttc acaacaacct
     2581 atttttagtt ccctattggg aaaacgacct gaatcttcac gacagtctat attctgcacc
     2641 caggatcatc agctgccgct acggtatcat caatgcttcg cataacctgg tcgagggtta
     2701 cgcccgctgg gagtccggtc agaatattga tgccgaaacc ggcgacggcg ctttccttgc
     2761 tttggacacc atgccccagt acaccatggc cgatctggaa ttgtcctggt ccgattttct
     2821 gaatcccgaa ggcggtgatt attcgtatat gtacaatatt cccctggcca ccgcaggcag
     2881 cgacggcggc cccataggcg acccccgctg ggtgatgact tttgccgatc ccaaaaacct
     2941 gacgcttgtc tccaatgccg aaggagcagt agttactccg gctatgggtg tgtacgaaag
     3001 cggaacggaa gtgaccattt cggcctccga agtggttggg tttaccttcc aaaactggca
     3061 ggatactttg ggcaacgtcg tctccgcaga aaacccctat acgtttaata ttacctccga
     3121 cacttatttg gtggcctatt acgaagaatt actctcgcgg gctatcacta taagattgaa
     3181 tggcaccaat accgccactt atagcttatc gccggcaagg gagatttatt acgtaggcga
     3241 cgaagtaact attaccgtca acgctcacta cctgaacgac ttcttgggtt ggagcgacgg
     3301 ctcaaaggaa ttatcgcgca gctttgtagt ggcaaacgac acaaccatcg ttgccaactt
     3361 tagacagtat ccctacatgc tggcctggga ct
//
LOCUS       DAOSGL010000252         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_26131_length_3385_cov_3.931832, whole genome shotgun sequence.
ACCESSION   DAOSGL010000252 DAOSGL010000000
VERSION     DAOSGL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26131_length_3385_cov_3.931832"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKY68_07605"
     CDS             <1..204
                     /locus_tag="PKY68_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOH24933.1"
                     /translation="IEIHVEVRPEFFSDHLSQMLNLKKKIAHRIQSVLGISAEIKLVE
                     PRSIERSTGKAKRVTDKRNIYNQ"
     gene            220..654
                     /locus_tag="PKY68_07610"
     CDS             220..654
                     /locus_tag="PKY68_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOH24934.1"
                     /translation="MLIKQLSIFLENKTGRLTEVTDLLGDAGINLSAFTIAESSDFGI
                     MRVIVSDPDKAKQILAQKGIAVSLTDVICLQCPNTPGALNKTLQLLSAGGISVEYMYA
                     FAVNEFAQVVIRPDKIQECISLLQENHYDLIKADDFYAFKEK"
     gene            735..1535
                     /gene="bamD"
                     /locus_tag="PKY68_07615"
     CDS             735..1535
                     /gene="bamD"
                     /locus_tag="PKY68_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOH24935.1"
                     /translation="MKTRFPLILLVILSLASCSEYQKLLKSQDYEAKWQKANEYYEQG
                     KYSKVIELMDELKTIYKGTERAEQALYMLGNSYFQNKDYFSASNEYQIYYKTFPNGTF
                     VEDCRFMSGKAAYLNAPDPRLSQEGTIQAMDELNVFLEYYPLSDRRAEVEIMLNDLME
                     KLAYKALLNCQLYYDLGNYMGNNYESCIVTAILALQDFPSSRYRVDLAFLILKSRYAF
                     AQNSYDYLKEERFRQVIDEYYSFVNEFPQSRYQKEAEQLFETAKKHVK"
     gene            1541..1879
                     /locus_tag="PKY68_07620"
     CDS             1541..1879
                     /locus_tag="PKY68_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOH24936.1"
                     /translation="MDYKKTNAPTSTQTRDLNALYDEGGNIYESVVVIAKRANQISME
                     LKEELKSKLQEFATYPDNQEEVSENREQIEISRFYEKLPKPTLIATQEFLEDKIYFRN
                     PLKEKSKSKA"
     gene            1876..2337
                     /locus_tag="PKY68_07625"
     CDS             1876..2337
                     /locus_tag="PKY68_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOH24937.1"
                     /translation="MIQRIQSLYLFVAAVLLTLALFMPWASFFGQEGELFQIKAFKVE
                     QILGKGEQSFPFWALGVLLIIAAALSFVCLFLFKNRRLQSRFCVFNTILLVGFYLVFL
                     FLVIHVRKSLDASLGINFGIALPLIALILNIMALRAIIKDEALVQSYHRLR"
     assembly_gap    2396..2405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2406..3003)
                     /locus_tag="PKY68_07630"
     CDS             complement(<2406..3003)
                     /locus_tag="PKY68_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOH24938.1"
                     /translation="MKRISLFYSFCILLPALCQNLRAQNKPFSPEFYLGATAGATMSS
                     ITLVPQYVDKTYLFAANGGLSLRYVNEKHFGIQAELNYFQSGWKDDFGYGSTNTYRRE
                     MAFLELPFLMHARIGTKAVNFYLNLGPKFSLFLSEEEYLDSDISYDYHGKALEQPFQW
                     GILGGVGFDVKLKRQNIGLEGRYYYGLSDIFANAVTDDF"
     gene            complement(3004..>3385)
                     /locus_tag="PKY68_07635"
     CDS             complement(3004..>3385)
                     /locus_tag="PKY68_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24939.1"
                     /translation="RITLLGYPEWLALSKNNLPQLYQLNTHVYSNYYADFQDQKLKQF
                     QKRYAYEFGKDLLNTFPRYALMGYDIMMYFVPKISGSEWETESLQHGFDFVRIGENGG
                     QCNRHLFIIQYTPQRKIISRALPR"
BASE COUNT         1025 a          699 c          723 g          928 t
ORIGIN      
        1 atcgaaattc atgtggaagt ccgtcccgag tttttcagcg accatttaag tcagatgttg
       61 aatctcaaaa aaaagattgc ccaccgcata caaagtgtct tgggcatctc ggccgaaatc
      121 aaactggtcg aaccgcgctc catagaacgt tccaccggaa aagccaagag ggtgaccgat
      181 aaacgtaata tttataacca gtaataagct acaactatga tgttaattaa gcaattatcc
      241 atctttttgg aaaataaaac cggacgcctt accgaagtga ctgatttact gggcgatgcc
      301 ggtataaact tatcggcttt caccatagct gaaagctccg atttcggcat tatgcgggtt
      361 atagtgtccg atcctgataa agccaagcag attttggccc aaaaaggtat agccgtatcg
      421 ctgaccgatg tcatctgcct gcaatgccca aatacgccgg gagcgttgaa taaaaccctg
      481 caactgctgt ctgcgggcgg aatttcggtg gaatacatgt atgcctttgc cgtgaacgaa
      541 tttgcacagg tggttatccg tcccgataaa attcaggaat gcataagtct cttgcaggag
      601 aatcactacg atctgattaa agccgatgat ttttacgcat ttaaagaaaa gtaaaaaacg
      661 gtcttgcatt tttataattg aagaatactc agtatatttg cgggcttttt tcagtagaag
      721 gtctcaatcg tagaatgaaa acaagattcc ctttaattct tctggttata ctaagccttg
      781 cctcttgcag cgaatatcaa aagctcctca agagccagga ttacgaagcc aaatggcaaa
      841 aggccaacga atattatgaa caggggaaat actcaaaggt gatagagttg atggatgaac
      901 tcaaaaccat ttacaagggg acggaaagag ctgaacaagc cttgtacatg ctgggtaact
      961 cctactttca gaacaaggat tatttttcgg cttccaacga ataccagatt tattataaaa
     1021 cttttcccaa cggaactttt gtggaagact gccgttttat gtccggcaaa gccgcctacc
     1081 tgaacgctcc ggatccccgg ctctcgcagg aaggaaccat ccaagccatg gacgaactga
     1141 atgtatttct ggaatactat cctctaagcg accgcagggc cgaagtggaa attatgttaa
     1201 acgacctgat ggaaaaactt gcctataaag ctttgttaaa ctgtcagttg tattatgatc
     1261 tgggtaacta tatggggaat aattacgaat cctgtattgt gacagccatc ctggctttgc
     1321 aggatttccc aagctctcgt tacagagtag acctggcctt cttgattttg aaatcgcgct
     1381 atgcttttgc tcaaaacagt tatgactacc ttaaggaaga acgttttcgc caggttattg
     1441 atgagtatta cagctttgta aatgaatttc cgcaaagcag atatcaaaaa gaggcggaac
     1501 aattgtttga aacagcaaaa aaacatgtaa agtaaataat atggactaca aaaaaacaaa
     1561 cgcaccaacc agcacacaaa cccgtgattt gaatgccttg tacgacgagg gtggcaatat
     1621 ttacgagagc gttgtcgtaa ttgccaaaag agccaaccag atcagcatgg aattgaagga
     1681 ggaactcaaa agcaaattac aggaatttgc cacctatccc gacaatcagg aagaagtatc
     1741 ggaaaatagg gaacaaattg aaatctcccg tttttacgaa aaattgccta agcctacttt
     1801 gattgccact caagaatttt tggaagataa aatctatttc cggaatcctc tcaaagaaaa
     1861 atcaaaaagc aaagcatgat tcagcggatt caaagcttgt atcttttcgt ggcagccgtc
     1921 ttgttgacgc ttgctctttt tatgccctgg gccagtttct tcggacagga aggggaatta
     1981 tttcaaatca aagcctttaa ggtagaacaa attctgggca aaggagagca gtcttttcct
     2041 ttttgggctc ttggagtttt gctgataata gctgccgctc tttctttcgt atgcctgttt
     2101 ttattcaaaa acaggcggtt gcagagccgc ttctgcgtat ttaacaccat tctgttagtt
     2161 ggcttttacc tggtatttct gtttcttgtt attcatgttc gtaaaagcct ggatgcaagc
     2221 ctggggatta attttggtat agctttacca ttgatagcgt tgattttgaa tataatggcc
     2281 ttgagggcca tcatcaagga cgaagctctg gtgcaaagct atcatcgtct caggtaatca
     2341 acgcaagcgg aaatagtata tcagatttaa agatatttgc tgcaggccgg aagtannnnn
     2401 nnnnncaaaa tcgtccgtca cggcattggc aaaaatatcg gataaaccgt aataataccg
     2461 gccttccagg ccgatgtttt gtcgttttag cttaacgtca aaacctactc ctcccaaaat
     2521 cccccattga aaaggctgct ctaaggcttt gccgtggtaa tcgtagctta tatcgctgtc
     2581 gaggtattcc tcttcggaca aaaagagact gaacttcgga cccagattca ggtaaaaatt
     2641 cactgccttg gtgccaatgc gggcatgcat caaaaaaggc agttccaaaa aagccatttc
     2701 tctgcggtaa gtgttggttg aaccgtaacc aaaatcgtct ttccagccgc tttggaaata
     2761 attcaattcg gcttggatac cgaaatgctt ttcattaaca taacgtaggc tcaggccgcc
     2821 attagcagca aaaagatagg ttttatccac gtattgagga accagggtaa tgctcgacat
     2881 agtggcgccg gccgtagcac ccagatagaa ttcggggctg aatggcttat tttgtgctct
     2941 aaggttttga cataaagccg gcagtagaat acagaagcta taaaaaaggc tgattcgttt
     3001 catttaacgg ggtaaagctc ttgagattat cttgcgctgg ggggtatatt gaatgataaa
     3061 caaatgccgg ttgcattgtc cgccattttc tccaatccga acaaaatcaa acccatgttg
     3121 taaactctcg gtttcccatt cggatcctga gattttaggc acaaaataca tcattatatc
     3181 gtaacccatc aaagcatagc ggggaaaagt gttcaaaaga tccttgccaa attcataagc
     3241 atagcgcttt tggaattgtt taagcttttg atcttgaaaa tcggcgtaat aattactgta
     3301 aacatgggtg ttcaactgat acaattgagg caagttgttt ttactcagag ccagccattc
     3361 cggatatccc aacaaagtaa tacgg
//
LOCUS       DAOSGL010000253         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_26229_length_3377_cov_3.168874, whole genome shotgun sequence.
ACCESSION   DAOSGL010000253 DAOSGL010000000
VERSION     DAOSGL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26229_length_3377_cov_3.168874"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..2650
                     /locus_tag="PKY68_07640"
     CDS             83..2650
                     /locus_tag="PKY68_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24940.1"
                     /translation="MRQHSSAVGRILFSGVLFCFSVLFSIQSSEKGFKDFFSSYNHFS
                     QAYTQEKVYLHFDNNSYFIGETIWFKAYVLLAENHHYSTMSKTLYVELLNSRGDVLIT
                     QKLKIENGQCSSGIFLPNEWYADYYEVRAYTRCMLNFGEGVVFSRVFPVMDAISYDKK
                     TGEPKPPAMTFSKSDLPIKRVDLKPQGNLSLDFFPEGGHLIEGLPSVVAFKAIGKQGE
                     DALISGNLYNSRKEVVTFFSSMHQGMGYLEFTPEPGQQYKAKVLYDDREYEFSLPAAL
                     PAGYALRVSNQNPAYLTIHIAAKSGEKNDSVGLAVTCRGKIYARAVILPQPEQILQIP
                     IQALPSGVQQITLFNRKGEILAERLAFVRNPFYDPLQYNISIKQLNENPDIFRPVRLE
                     VELETARNSRLTDSNTVKTLPAGTSLVLSVRDAAENLKNAPLRNIYSDLLLSSELKGY
                     IHNPDWYFEKEDLRRSKALDLLMLVQGWRRYDWEIMAGSKPFEAKHPIEEGIVLQGEI
                     ISQLLKKPRKDIRVTIWMTSDSSAYYGNCLTDSAGTFNFLFDFVDTWKLSLQITDGEK
                     RKNHRIVLDRLFSPAPKSFAYYEREAPVYERLRFPKKTVAGRLPSVIDSSKITVLPGE
                     VLLPQAEIKRRRSEQLTTGEASVIYAVEREVDVIRDRAENEYSSIPIFLTQINPYFWL
                     GQKYSSSSDSMEDVMQYKGKDVVFVLEDLYTIGMNSEEMPYVDEVENIAIVESNTPNL
                     FYESPEAVATMKSRVYVHLYRYKDRRRNIDPLGIRKTTLQGYSRVKEFFHPKYDRVLM
                     SDPQDHRRTLYWNPSLTTDSTGKAQVLFYNNASCRKMNFDAVLLTEDGKMGLLKAEP"
     gene            complement(2735..3377)
                     /locus_tag="PKY68_07645"
                     /pseudo
     CDS             complement(2735..3377)
                     /locus_tag="PKY68_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
BASE COUNT          947 a          801 c          780 g          849 t
ORIGIN      
        1 ctgtatccgg gcacttaccg ggccgatttc tttgccgatg gattccttat cggcagcaag
       61 tctttcgagt tgctcaacta acatgagaca acattcctca gcggtgggac gcatcctgtt
      121 ttcaggagta ttattctgct tttcggtatt attctcaatt caaagcagcg aaaaaggctt
      181 taaggatttt ttcagttctt ataaccattt cagccaggct tacactcagg aaaaagtcta
      241 tttacatttc gacaacaact cttattttat aggtgaaacc atttggttca aagcctatgt
      301 gctcctggcc gaaaaccacc actattccac catgagcaag accctttacg tggaattgct
      361 caacagcagg ggagatgtgc ttatcacaca aaaattaaag atagaaaacg gacaatgcag
      421 cagcggtatt tttttgccca acgagtggta tgcagactat tacgaagtaa gagcctacac
      481 tcgttgcatg cttaactttg gagaaggtgt ggttttttcc cgggtctttc cggtaatgga
      541 cgccatcagt tacgacaaaa aaaccgggga acccaagcct ccggctatga ctttcagcaa
      601 aagcgacctg ccgataaaaa gagtggatct taaaccgcaa ggaaatctga gtctggactt
      661 ttttcccgaa ggaggtcatt taattgaagg cctgccctca gttgttgcat tcaaagcaat
      721 aggcaaacaa ggtgaagacg ctttgatatc gggcaatctg tataacagcc gcaaagaagt
      781 ggttactttt ttcagtagta tgcatcaggg aatggggtat ctcgagttca cccccgaacc
      841 cgggcagcaa tataaagcaa aagttttgta cgacgaccgg gagtatgaat ttagtttgcc
      901 ggcagccctg cccgcgggct atgctctgag ggtaagcaat caaaatccgg cttatttaac
      961 catccatatc gcagcaaagt ccggtgaaaa aaacgactcc gttggcctgg cggtgacctg
     1021 ccggggtaaa atctatgccc gggcggtcat cttgccgcaa ccggaacaaa ttcttcagat
     1081 acccatacaa gcattgccct cgggtgtgca acaaatcaca cttttcaacc gaaaaggaga
     1141 aattctggcc gagcgcttgg cttttgttag gaatccgttt tacgacccct tacagtacaa
     1201 catttccata aaacaactga acgagaatcc ggatattttt cgtccggtca ggctcgaagt
     1261 ggaattggaa accgcccgga actcccggct gacggacagc aatacggtca aaacgctgcc
     1321 ggccggcacc tccctggtgt tgtcggtacg cgacgccgcc gaaaacctga agaatgcgcc
     1381 cttgagaaat atatacagcg atttattgct ttcttccgaa ctcaagggtt atatccacaa
     1441 tcctgactgg tattttgaaa aagaagatct gagacgaagc aaggctctcg atttactgat
     1501 gctggttcag ggctggagac gctacgactg ggaaataatg gcgggaagta aaccgttcga
     1561 agccaaacat cctatagaag aaggtatcgt attacagggt gaaattattt cccagctatt
     1621 gaaaaaaccc agaaaagaca ttcgggtaac catctggatg acttccgaca gctcagccta
     1681 ttacggaaat tgcctgaccg attcggcggg aacctttaat ttcctgtttg attttgtgga
     1741 tacctggaag ctttcgctac aaatcacgga tggggaaaaa cgcaaaaacc accgtatagt
     1801 gctggacagg ctattcagcc ctgcaccaaa atcttttgct tattacgagc gtgaagctcc
     1861 cgtttatgaa cgattgcgat ttcctaaaaa gacagtcgcc ggcagactgc caagtgtcat
     1921 agacagttcc aaaatcaccg tactgccggg agaagtctta ttgccgcagg cagaaataaa
     1981 aaggagacga tccgagcaac ttactaccgg agaggccagc gtaatatacg ccgtcgaaag
     2041 ggaagtagac gtaatccggg atcgggccga aaatgaatac tcaagcattc ccatcttcct
     2101 gacccagatc aatccctatt tttggctagg ccaaaaatac agctcttcca gcgactccat
     2161 ggaggatgtt atgcaataca aaggcaagga tgttgttttt gtcctcgaag atttatacac
     2221 cataggtatg aattccgagg aaatgcctta tgtggacgaa gtcgaaaata ttgccatagt
     2281 ggaatccaac actcccaacc ttttctacga aagccccgaa gcagtggcca ctatgaaaag
     2341 ccgggtttat gtgcatctct accgctacaa agaccgccgt cgcaatatcg accccttggg
     2401 catccgtaaa accactttgc agggctacag ccgggtaaag gaatttttcc atcccaaata
     2461 cgatcgcgtc ctgatgagtg atccacagga ccacaggcgc actttgtact ggaatccgtc
     2521 gctcacaacc gacagcacag gcaaagccca ggtgcttttt tataacaacg caagctgcag
     2581 aaaaatgaat ttcgacgccg tcttgctgac cgaagacggg aaaatgggat tactcaaggc
     2641 tgaaccctga ttttttttga cttctttggg ctcggaggac ttattcgaga ttgggtgcta
     2701 ttcgggctcg ggtggctggg gttcgggaga cttattccgg ctcgggaaaa tgaatgtccg
     2761 aacgtcccac aggtctggac gttgccacgg caataccccg ctgctcgtct ttatttaccg
     2821 cacaataaaa atgatatacc gtcccttggt attcgattaa aaaagatttg tgcgcgaata
     2881 agtcatcgta atcttcggag ggtattatca gatcgggtcc ctgccattct gtccagttga
     2941 tcaaatcgta ggaacaggca aaagtattga aagccttata aggcctgtct ttccaaaaag
     3001 caccaaaata aaacatcaca tagagatcgc ccattttctg gatgtgggca tctcctgtaa
     3061 tcatgccatc ctcattaccg aaaaccggat tgcccggata acgctcccaa tggagcatat
     3121 cgttggataa agctataccg atacgttcgg ctttaacatt ggtttgggga tgatagcctg
     3181 ctgcgttata aaacatcata aagcgggctc ccaggctttt cttttcatca cggtaaacca
     3241 aacttttgta ttgcgtgatt gtttcaaacc actgggcgtc ttcgtctttg ggagacatca
     3301 gaggttgcgg aaaagttttc cactcgtggg cggtacttat atctccctcg gtataagcca
     3361 atccaatttg caaggtg
//
LOCUS       DAOSGL010000254         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_26466_length_3356_cov_3.317177, whole genome shotgun sequence.
ACCESSION   DAOSGL010000254 DAOSGL010000000
VERSION     DAOSGL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26466_length_3356_cov_3.317177"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..438
                     /locus_tag="PKY68_07650"
     CDS             157..438
                     /locus_tag="PKY68_07650"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HOH24941.1"
                     /translation="MHFTSVELIGLAAAMFSTVNQMPQAWKIIRSKDTGSISLYTYSL
                     LWIAVTLWLIYGIVIRDKPLIISNIISIVPITYIYFIKLKNTLSRKDSI"
     gene            complement(435..1220)
                     /gene="tatC"
                     /locus_tag="PKY68_07655"
     CDS             complement(435..1220)
                     /gene="tatC"
                     /locus_tag="PKY68_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOH24942.1"
                     /translation="MTEEKLTFWEHLDELRVVLIRIVIVLAAVTVIAFAFKEPLFKII
                     LAPHKADFILYRLINRLSSLLAWPQMSLPEFEVKLISTELTAQFFTHMKVAFFAALLV
                     SSPYVVFILFRYISPALYHNEKKYSGRVLFWAYSLFFLGVLLSYYIIFPLSFRFLGTY
                     QVSPEVVNTITLNSYVQTLLVLSLLMGIMFEIPVLAWFFAKIGLLKADFMRKKRRYAI
                     VIILIVAAVITPTGDVFTLTIVSLPVFLLYEISILLVAKTSGA"
     gene            complement(1233..1439)
                     /gene="tatA"
                     /locus_tag="PKY68_07660"
     CDS             complement(1233..1439)
                     /gene="tatA"
                     /locus_tag="PKY68_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOH24943.1"
                     /translation="MYYLPLLLGLGTSELILIALVILLLFGATRIPEIMKGIGKGIKS
                     FKKEMNSTDDEEPLNNKKEKDSDK"
     gene            complement(1457..3100)
                     /locus_tag="PKY68_07665"
     CDS             complement(1457..3100)
                     /locus_tag="PKY68_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH24944.1"
                     /translation="MLRKLRIALALVAFAVLTFFFLDFSRLLPESAQVIAKIQFVPAL
                     ISLSLGIVLLLLLLTLVFGRIYCSVICPLGIYQDVISRLANLFRKKKLRYKFRKARTI
                     LRWSVLTLVIITYLSGLGLVLDLLDPYSMYGRMAVHLFKPVYMAANNGLELLFTAFNN
                     YTFYKVDIHFWGWASFGLALISLLVVSVLAWRFGRTWCNTICPVGTVLGFLSKFSVFK
                     LSIDHETCNQCGLCSFNCKATCIDSKKGSIDYSRCVACFNCMESCNKKGVKYLPFWKV
                     KKTGTDKTQVKTVSGKIQAKTKTKTKTKKRIETNTDTKIEVKTINKTKTDASKREFLS
                     VLMMSSLLMPKALAAGFRQQISSLRPLKRTVPMMPPGAGDRARFNSLCTSCHLCVSKC
                     PGDVLQPSLLEYGVEGIMQPQMLFDKGFCNYDCTLCTEVCPSGALKTLTKEEKHRTQM
                     GRVVFVIENCIVYRNETHCGACSEHCPTQALRMVPYKGHLTIPEPHPDICIGCGGCEF
                     ICPVRPYKAVFVEGHEIQQVAKAFEDEEKQDIVLDDFGF"
     gene            complement(3111..>3356)
                     /locus_tag="PKY68_07670"
     CDS             complement(3111..>3356)
                     /locus_tag="PKY68_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tat (twin-arginine translocation) pathway signal
                     sequence"
                     /protein_id="HOH24945.1"
                     /translation="IVDAYRVLQTNGPQGRSESDVVLSKALFVSPEIVSVDTAATSFF
                     NQIRSMPMDVVKHLELGEELGLGTTDLNKLNIKRIRL"
BASE COUNT         1052 a          719 c          660 g          925 t
ORIGIN      
        1 cctcaaaatc atagtattga ttctgccagg caaagataca aattaattta tttacacaag
       61 gccgggtcga ttaatttgct tgccgaaaaa aactttaaat cctaagccaa aaaaacctga
      121 tatctttgtg tcttattgac cttagattaa aagaatatgc atttcacctc agtagaactg
      181 ataggactgg cagcagccat gttttcgacc gtaaaccaaa tgcctcaggc ctggaaaata
      241 atccgtagta aagacaccgg tagtatctcg ttatatacct atagtttgct ttggatagcc
      301 gttactttat ggctgattta tggaattgtc attagagaca aacccctgat aatttcgaat
      361 attatcagca ttgttcccat tacctacatt tattttatca agctcaaaaa caccttatcg
      421 cgcaaagaca gtatttaagc cccggatgtt tttgccacca gcaagatgct gatttcgtaa
      481 agcaggaaaa cgggtaaaga gacaatcgta agagtaaaga catcgccggt tggagtaatg
      541 acggcggcaa caatcagaat tataacaatg gcgtacctgc gtttctttct cataaaatcg
      601 gctttaagca gaccgatttt agcaaagaac caggccaata ccggtatctc aaacattata
      661 cccatcagga gcgataatac caacagagtt tgcacatacg aattcagggt aatggtatta
      721 accacttccg gactaacttg ataagtgccc aaaaaacgaa aagagagcgg gaaaataata
      781 tagtagctca gcaacacacc aagaaaaaac aaactatagg cccaaaagag tacacgtccg
      841 gaatattttt tttcattgtg gtaaagagcc ggcgaaatat aacgaaagag aataaatact
      901 acgtacggag aactcaccag tagagctgca aaaaaagcaa ctttcatatg cgtaaaaaat
      961 tgggcggtaa gttccgtact aattaattta acttcgaatt cgggcaggga catctgaggc
     1021 caggcaagca aggaagacaa tcgattgata agcctgtaga gtatgaaatc ggccttgtgt
     1081 ggcgccaaga ttattttaaa aaggggctct ttgaaggcaa acgcaatcac agtcacagct
     1141 gccaaaacta tgactatgcg aattaaaaca actctgagtt cgtctaaatg ttcccagaat
     1201 gtaagcttct cctccgtcat gcagcttttt tattatttat cgctgtcttt ttcttttttg
     1261 ttgttaagcg gttcttcgtc gtcggtacta ttcatctctt ttttgaaact cttgataccc
     1321 ttgcctattc ctttcatgat ttcaggaatt ctggtggctc caaacaataa gaggattacc
     1381 agtgcaataa gaattagttc tgaggtacct aaaccaagta ataaaggcag gtaatacata
     1441 atcgttatta ttaatgttaa aatccaaaat catccaggac aatatcttgc ttttcttcgt
     1501 cttcgaaagc tttggcaact tgttgaatct catgtccttc tacaaaaaca gccttatagg
     1561 gccttacggg gcaaataaat tcgcaacccc cgcaacctat gcaaatatcg ggatgcggtt
     1621 ccggaatggt cagatgccct ttatagggca ccattctaag cgcctgggtg ggacaatgtt
     1681 ccgaacaggc tccacaatgg gtttcattac gataaacaat gcagttctca atcacaaaaa
     1741 caacccttcc catttgtgta cgatgtttct cttctttagt cagagttttc agggctccgc
     1801 tgggacacac ctcggtacac aaggtgcagt cgtagttgca aaagcccttg tcaaacaaca
     1861 tttgaggttg cataatacct tcgacaccgt attccagcaa gctgggttgc agcacatcgc
     1921 ccgggcattt gctgacacag agatgacagg aagtgcacaa actattgaaa cgagctctgt
     1981 ctcctgcgcc cggaggcatc ataggaacag ttctctttag agggcgtagg ctgctgatct
     2041 gttgacggaa gccggcagcc aaagctttgg gcatcaataa actgctcatc atcaagaccg
     2101 ataaaaattc tcttttggaa gcatccgttt ttgtcttgtt tattgttttt acctcaatct
     2161 ttgtatcagt attggtttca attctttttt ttgtttttgt ttttgttttg gtttttgcct
     2221 gaattttgcc gcttactgtt tttacctgag ttttgtcggt ccctgttttt tttactttcc
     2281 aaaaaggcag atactttacg ccttttttgt tacaactctc catgcaattg aaacaagcga
     2341 cgcaacggct gtaatcgatg cttccctttt tactgtctat acaggtagcc ttacaattga
     2401 aactgcaaag cccgcattgg ttacaggttt cgtgatcaat actcaatttg aagacggaaa
     2461 acttgctcaa aaagcctaaa acggtaccca ccggacaaat ggtattgcac caagtgcgac
     2521 caaaacgcca ggccaaaacg gagaccacta ataagcttat aagggcgagt ccaaaagaag
     2581 cccaacccca aaaatgaata tcgactttat aaaaagtgta attgttgaag gcagtaaata
     2641 aaagctccag gccattgtta gcagccatat atacaggctt gaataggtga acggccattc
     2701 ttccgtacat actgtaagga tccagcaaat ccagtaccaa tcctaggccg cttaggtagg
     2761 tgataatcac caaggttaaa accgaccagc gtaaaatcgt tcttgcctta cgaaatttat
     2821 accggagttt tttcttgcga aatagattgg ccagcctgga aatcacatct tgataaatgc
     2881 ccaagggaca aataacagaa caatagattc tgccaaaaac caaagtgagc agcaacagca
     2941 gtaaaacaat tcccagtgaa agcgaaatca aagcgggaac aaactgaatt ttagctataa
     3001 cttgtgcact ctccggcaga agacgggaaa aatccagaaa aaagaatgtt agtactgcaa
     3061 aggctaccag ggccaaagct attctaagct ttctaagcat tatgatttaa ttaaagcctg
     3121 atccttttaa tgttcaattt gtttaaatcg gtagttccca ggcccagttc ttcgcctaat
     3181 tccagatgtt taaccacatc cataggcatg gaacggattt gattgaaaaa agaagtggcc
     3241 gcagtatcga ccgacacaat ttccggggaa acaaacaagg cttttgataa aacaacgtcc
     3301 gactcggaac gcccctgtgg tccgttagtt tgtaaaacac gataggcatc tactat
//
LOCUS       DAOSGL010000255         3351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_26521_length_3351_cov_4.461165, whole genome shotgun sequence.
ACCESSION   DAOSGL010000255 DAOSGL010000000
VERSION     DAOSGL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26521_length_3351_cov_4.461165"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKY68_07675"
     CDS             <1..326
                     /locus_tag="PKY68_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOH24946.1"
                     /translation="GAFIRDHRADLCASLQATIVDILMDKLIKASKDLNIKEIALSGG
                     VSANSGLRRAVEETALLKGWTIHLPPLSFTTDNAAMIAVGGYYKFLDRDFCGMDAAPY
                     SRVSI"
     gene            337..1605
                     /locus_tag="PKY68_07680"
     CDS             337..1605
                     /locus_tag="PKY68_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOH24947.1"
                     /translation="MDSSITPSYKNVEIITIGDEIINGQIVDSNSAWIASFLNRSGFE
                     VDKITSVPDDAQSIFRAVDSALGSSPIVLLTGGLGPTKDDITKHCLCSYFKTKLVFNQ
                     AVYDNMTGLFNKRGLRRINDFNRSQAEVPEACTVIVNRKGTAPVTWFEREGKILVSMP
                     GVPLEMMWCMEKEIMPRLLNKVQSHPILQRYIWVKGYPEAVLAEFISDWEDALPPELK
                     LAYLPQRSVMLLRLTAKNNDETTLIGLLETQLAGLRSRLGSAIFAEGVKTLEEALGQV
                     LIKKGLKLALAESCTGGAVGARLTRAPGSSTYFLGGIISYANELKQQWLGVPETLLQT
                     KGAVSKEVAEAMAAGVRIKLKSDIGIALSGIAGPTGGSAEKPVGTVFIALADNDRCLS
                     RKFEFQSDRLGNIEQSVHQALILLLDYLYL"
     gene            1766..2026
                     /gene="rpmB"
                     /locus_tag="PKY68_07685"
     CDS             1766..2026
                     /gene="rpmB"
                     /locus_tag="PKY68_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOH24948.1"
                     /translation="MSKVCQITGKRAMIGNNVSHSKRRTKRKFNVNLFEKKFYWVEQD
                     CWIALTISAAGLRIINRKGLNQAIKKAGEDGFLRLEEVKILN"
     gene            2038..2226
                     /gene="rpmG"
                     /locus_tag="PKY68_07690"
     CDS             2038..2226
                     /gene="rpmG"
                     /locus_tag="PKY68_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOH24949.1"
                     /translation="MSKKAKGNRVQVILECTEHKESGMPGTSRYITTKNRKNTTARME
                     LKKYNPILKKVTVHKEIK"
     gene            2235..2387
                     /locus_tag="PKY68_07695"
     CDS             2235..2387
                     /locus_tag="PKY68_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOH24950.1"
                     /translation="MAKKTVATLHDVGSRNMVKVIKMVKTPETGAYSFQEEMVPVEKV
                     NEYVKK"
     gene            2467..>3351
                     /gene="ftsY"
                     /locus_tag="PKY68_07700"
     CDS             2467..>3351
                     /gene="ftsY"
                     /locus_tag="PKY68_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOH24951.1"
                     /translation="MGIFSFFSKDKKEKLDQGLSKTKESFFTKVNRAIAGKSKVDDQV
                     LDELEEILIGSDVGVDTTLRIIERIEKRIERDKYLNTDELYSILREETVELLIENDQE
                     ASKDFIIPEDTKPYVIMVVGVNGVGKTTTIGKLAYQYTQRGKKVFIGAADTFRAAAIE
                     QLCVWGERAGANVVKQAMGSDPASVVFDTLNSALSNQADVVLIDTAGRLHNKINLMNE
                     LTKIKKVMAKLIPEAPHEILLVLDASTGQNAFEQAKQFSLATEVNALAITKLDGTAKG
                     GVVLGISDQFRIPVKYIGL"
BASE COUNT         1037 a          685 c          790 g          839 t
ORIGIN      
        1 acggagcttt tatccgggat caccgagccg atttatgcgc ctctttgcag gccactattg
       61 tggacatctt gatggacaaa ctcatcaaag ctagtaagga tttaaatatc aaagaaatag
      121 ctttatccgg aggcgtatcg gcaaattccg gcttgcgcag agcggtagaa gaaacagcct
      181 tattgaaagg ctggaccata catcttccgc ctttgtcttt tactaccgac aatgccgcca
      241 tgattgccgt aggaggctat tacaaattcc ttgatcggga tttttgcgga atggacgcag
      301 cgccctattc cagagtcagc atttaaatag accaccatgg acagtagtat tactccgtcg
      361 tacaaaaacg ttgaaatcat cacgataggt gacgaaatca tcaacgggca aatagtagac
      421 agtaattcag cctggatagc ttcttttttg aatcgctcgg gctttgaagt ggataaaata
      481 acttcggttc ccgacgatgc gcagtctatt tttcgggctg tggattcagc cctgggcagt
      541 tctccgattg ttttgctgac aggcggctta ggcccaacca aagacgatat taccaagcat
      601 tgtctatgtt cctatttcaa gactaaactg gttttcaacc aggctgttta tgataacatg
      661 accgggctgt tcaataagcg gggcctacgg agaataaacg atttcaatcg ttcacaggcc
      721 gaagtccccg aagcatgtac agtcatcgtc aaccgcaagg gcacagcgcc ggtcacctgg
      781 tttgaacgag aggggaaaat tctggtttcc atgccgggag ttcctctgga aatgatgtgg
      841 tgcatggaaa aagagatcat gccgcgcctg ctaaacaaag tgcaatcgca tcccatcctt
      901 caacgataca tttgggttaa aggctacccg gaagctgttt tggccgaatt tatttccgat
      961 tgggaagacg ctttgcctcc ggagcttaaa ctggcttatc ttcctcagcg ttcggtaatg
     1021 ctgttgcgcc ttaccgcaaa gaataatgac gaaacaacat tgatcggtct tttggaaacg
     1081 cagcttgccg gcttaagaag ccggctggga tctgccatat ttgccgaagg ggttaaaacc
     1141 ctggaagaag ccctgggaca agtattaata aaaaaaggac ttaaattggc tttggccgaa
     1201 agctgtacgg gaggagcggt cggagccagg cttaccaggg ctcccggcag ttcgacttat
     1261 tttttaggcg gcatcatctc atacgccaac gagcttaaac aacaatggct tggggtgccg
     1321 gaaaccctgt tgcagaccaa aggagcggtg agtaaggagg tggctgaagc tatggccgca
     1381 ggagtcagaa ttaaattgaa aagcgatatc ggcattgcat tgagcggaat agccggcccc
     1441 acaggaggca gtgctgaaaa gccggtcggc accgttttta ttgccttggc cgacaatgac
     1501 cggtgtttat ccagaaaatt tgaatttcaa tcggataggc taggaaacat tgaacagtcg
     1561 gtgcatcagg ctttgatttt gttattggat tatttgtact tgtaaatctg tctcttaagc
     1621 tcaaactgta ttttttataa aatttattcc ctcatttttg gatatttgtc aaaaaaagat
     1681 gatatttgca cccgcaaaaa tctattcttg ttactcgcac acaagaggat gcaaaagcaa
     1741 ttaaacacaa aaacaacata cagcgatgtc aaaagtttgt caaattacag gaaaaagagc
     1801 aatgattggc aataacgtct ctcactctaa gagacgtaca aaacgcaaat tcaatgtcaa
     1861 cttgttcgaa aagaaattct attgggtaga acaagactgt tggattgcat tgaccatttc
     1921 tgctgcagga ttgcgcatca tcaaccggaa aggtttgaat caggctatca agaaggccgg
     1981 ggaagatggt ttcctgcgtt tggaagaagt aaaaattctt aattaggagg agtactcatg
     2041 tcaaagaaag caaaagggaa cagagttcag gttatcctgg agtgcacgga acataaagaa
     2101 agcggtatgc ccgggacctc ccgctatatc actactaaaa accgcaaaaa tacgactgcc
     2161 agaatggaat taaaaaaata caatcctatt ctcaagaagg tcactgtaca taaagaaatt
     2221 aaataaatag acacatggca aagaaaacag tagctacctt gcatgatgtc ggtagccgta
     2281 atatggtgaa agttattaag atggtgaaaa cgcccgaaac aggagcctat tctttccagg
     2341 aagaaatggt accagtagaa aaagtcaacg aatacgttaa aaaataaata gggctgttta
     2401 tttctgacta taaaggttgt tttttcacga aaacagcctt tttgtgcata tataacaagg
     2461 gaatgtatgg gtattttcag ttttttttct aaagataaaa aggaaaagct cgatcagggt
     2521 ttatctaaaa ccaaggaaag ctttttcacg aaagtgaatc gtgccatcgc cggtaaatca
     2581 aaagtggacg accaggtact ggatgaactg gaggaaattc tgattggatc cgatgtagga
     2641 gtggatacta ctctaaggat tatcgaacga atagaaaagc gcattgaaag agacaaatac
     2701 ctgaataccg atgaattata ctctattttg cgcgaagaga ccgttgaatt actcatagaa
     2761 aacgatcagg aagcttccaa agattttatt atacccgaag acaccaaacc ttacgtcatt
     2821 atggtggtag gtgtcaacgg tgtaggaaaa accaccacca tcggcaaact ggcttatcaa
     2881 tacacacagc gaggcaaaaa agtgttcatt ggagcggccg acacattcag ggcggcagcc
     2941 atcgagcaat tatgtgtatg gggcgaaagg gcaggagcga atgttgtaaa acaagcaatg
     3001 ggctcggatc cggcttcggt agtattcgat accctcaatt cggcgcttag caaccaggca
     3061 gacgtggtac ttatcgatac cgccggcagg ttacacaaca aaatcaacct gatgaatgaa
     3121 ctgacgaaga tcaaaaaagt aatggcaaag ctgattcctg aagctccaca tgaaattttg
     3181 ctggttttgg atgcatccac cggacaaaat gcctttgaac aggccaaaca attcagtttg
     3241 gctaccgaag tcaatgctct ggctattacc aaactcgacg gcacagccaa gggtggtgtg
     3301 gtgctgggca tctccgatca attcagaatt cctgttaaat acattggatt g
//
LOCUS       DAOSGL010000256         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_26572_length_3347_cov_3.487849, whole genome shotgun sequence.
ACCESSION   DAOSGL010000256 DAOSGL010000000
VERSION     DAOSGL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26572_length_3347_cov_3.487849"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..2331
                     /locus_tag="PKY68_07705"
     CDS             127..2331
                     /locus_tag="PKY68_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH24952.1"
                     /translation="MTISEIDSLYARIFRFLYQMRIKEAITDLGYLIQQNGFGPAYDE
                     LVQQETTYRYLLRYRLEGVEDKERDKVLLKLIRTLCELADSAYRQWLTVNSSQWYYSR
                     LRAYRSAKNTQEVLEELRELDAELALTSLKKDEDQRKAEMLQLNSRREQLSGTLFQRL
                     LLSDIWSDREVKLLQEAYAHLLYSHEKALLVSALLLSLQFLFDEKKFLFLLDLCEHEE
                     VEVNRRALTAVMLLFDMYDRRMQWYPAISSRLAALLEDEARQSALLSIFIQLARTKDV
                     DTISRRMQEEFLPEMSRLGSSIQQKITESQEFGEELNPDWKELFEDASLSEKMQEFSE
                     MQIEGVDVYMSTFSNLKFYPFFKEIHNWFLPFYSEHSQVAELFKHDSLQGVNVTKLVT
                     SSDFLCSSDKYSFCFNLAQVPETYRSQMAANLGSETEAYQELQKMEQSSNQRLKTESV
                     SNRYIQDLYRFYKLFSHKKDFKDVFAGSLDFYAVSGISPYLEQEEIMLKVALVYFKTK
                     HYRQALKLFDRLLENKAGDADLHRKKAYCHQQLQEYAIALQAYLKADLLQHDNLWTLK
                     RISACYRQLKNNEEALVYYRRAEKLDPENYVLSMNIGHCLMDLQRYNEALQSYYKAEF
                     ISGESPRTWTPLVWCAIMCKKYDQAGKYVQKLLAHKPVMVDFLNAGHLEWLQGSALKA
                     IQMYKKGIRLTHTAQSEFFDLFEKDLPVLLEQGINPADIPVVRDGLLYQLEE"
     gene            2335..3222
                     /locus_tag="PKY68_07710"
     CDS             2335..3222
                     /locus_tag="PKY68_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24953.1"
                     /translation="MPVSVIFRILAGCLCFAFSGLSAHAKDSPTVLFKAAWLKNEAQS
                     YTLEHKTYRLQQGDTSELETISLRADVTVRDSTYDRYVLVWSFCDFSMQSSHYPSVWL
                     MQHIKDLKISCRTTTAGVLKEFPDLDDFRKGLDRSLESAFASYQGLPSEASRARVYRL
                     REELESFLLMTIHHVHQAYGLGYTPGEIVEVPEKMNIRFSETAIDAVVRKKLDGYDPE
                     SGVAGLVMATVLDTAQLNKAMRPLSSALIQPVYKQENTSALVMHIPSGWPLYGFDKRE
                     LTQDRNLYGEFFEITHRQK"
BASE COUNT          925 a          772 c          803 g          847 t
ORIGIN      
        1 gatacaaaag ttgcgaatac ggtacatcct atagtgctta tatattttga ggctgcaaaa
       61 ttactaaaat ttatgcaagt gccggctaaa ttctttgtat ttttgtggct gataaagcag
      121 tagaacatga caatttcaga aattgattcc ctatacgcga gaatttttcg tttcctgtat
      181 caaatgcgca tcaaagaggc catcaccgac ctgggctacc tgatccagca aaacggcttc
      241 ggtccggctt acgatgaatt ggtacaacag gaaaccacct accgctattt gctgcgttac
      301 cggctcgaag gtgtggaaga caaggaacgc gacaaagtct tgctcaaatt gataagaacc
      361 ttgtgtgagt tggccgattc tgcttatcgt caatggctta cagtcaattc aagccaatgg
      421 tattattccc gcctcagagc ttaccggtct gccaaaaaca cgcaggaagt cctggaggaa
      481 ctcagagagc tggatgccga acttgctctg acatcgctta aaaaggacga agaccagcgc
      541 aaagcggaga tgctgcaact aaacagccgg cgcgaacaac tgagcggaac gctcttccaa
      601 cggctgttat tatcggatat ctggagcgat cgggaagtca aacttttgca agaagcctat
      661 gcccatttgc tctattctca tgaaaaggcc ctgctcgtat cggccttgtt attatcgctg
      721 caattccttt tcgacgaaaa aaaattcctt ttcctcctcg atttatgcga acacgaagag
      781 gtagaagtca accggagagc cctcaccgcc gtgatgttgc tttttgatat gtacgatcga
      841 cgtatgcagt ggtatccggc cataagcagc aggttggccg ccttgctcga agacgaggcc
      901 aggcaatcgg ccctgttgtc tatttttatc caactggcca gaaccaagga tgtagatact
      961 atcagccgca ggatgcagga agaatttcta cccgaaatga gcaggctggg ctcgtctatt
     1021 cagcaaaaaa ttactgaaag ccaagaattt ggcgaagaac tcaatcccga ctggaaagaa
     1081 ttgttcgaag atgcttccct gtcggaaaaa atgcaggaat tctccgaaat gcaaatcgaa
     1141 ggggtggacg tgtatatgag tactttttcc aacctgaagt tttatccctt tttcaaagaa
     1201 atacacaact ggtttttgcc cttttacagc gaacacagcc aggtggcgga actttttaaa
     1261 cacgacagct tgcagggggt gaatgtgacc aaattggtta cttcctccga tttcttatgt
     1321 tcttccgata aatattcctt ttgtttcaat ttggctcagg tgcccgaaac ctaccgcagt
     1381 cagatggctg ccaacctggg atccgaaacc gaagcctacc aggaactgca aaagatggag
     1441 cagagcagca atcagcgtct gaaaaccgaa tcggtctcca atcgctatat ccaggactta
     1501 tatcgttttt ataaattgtt cagccacaaa aaagatttca aggatgtctt tgccggttct
     1561 ctcgactttt acgcagtttc cggaatcagc ccctacctgg agcaagagga gattatgttg
     1621 aaggtggcgc tggtttattt caaaaccaag cattacaggc aagctttgaa actttttgac
     1681 cgtctgctcg aaaataaggc cggcgatgcc gacttgcacc ggaaaaaagc ctattgccat
     1741 cagcagttgc aggaatacgc catagccttg caggcctacc tgaaggcaga tttgttgcag
     1801 cacgacaatt tatggacatt aaagcgcatt tctgcttgtt accgtcagct caaaaacaac
     1861 gaagaagcac tggtctatta ccgcagagcc gaaaaactgg atccggagaa ttatgtgctc
     1921 agcatgaaca taggccattg cctgatggat ttacagcgtt acaacgaggc actgcaaagt
     1981 tattacaagg ccgaattcat ttccggagag tcgcccagaa cctggacgcc cttggtctgg
     2041 tgcgccatta tgtgcaaaaa atacgaccag gccggtaaat acgttcaaaa gctgttggca
     2101 cacaaacctg tgatggtcga ttttctgaat gccgggcatt tggaatggct gcagggatcg
     2161 gctctgaaag ccatacaaat gtataaaaaa ggcatacggc tcacccatac ggcccagagt
     2221 gaattttttg atcttttcga aaaagacttg cccgttttat tggaacaggg gatcaatccg
     2281 gccgatattc ccgtggtcag agacggttta ctgtatcaac tcgaagaata aattatgccg
     2341 gtttcagtta ttttcaggat ccttgcgggc tgtctgtgct ttgcgtttag cggtttaagc
     2401 gcgcatgcca aagacagccc aacggttttg ttcaaggcag cctggttgaa aaacgaagca
     2461 cagtcgtata cactggaaca caaaacctac cgtctgcaac agggagacac ctccgaactg
     2521 gagaccatca gcctgagggc cgatgtgacc gtgcgggatt ccacctacga ccgctacgtt
     2581 ttggtatgga gcttctgcga tttttcgatg caaagcagtc attatccttc ggtctggctg
     2641 atgcagcaca tcaaggattt aaaaatcagt tgccggacta caaccgccgg tgtgctcaaa
     2701 gaatttcccg acttggatga ttttcgcaaa gggctggacc ggtctctgga atcggctttt
     2761 gcttcgtatc agggtctgcc ctccgaggct tccagagcca gggtttaccg gctcagggaa
     2821 gaattggaga gctttttatt gatgaccatc caccatgttc atcaggccta tggactgggc
     2881 tatactcccg gagaaatagt cgaagtaccc gaaaaaatga atatacgttt ttcggagaca
     2941 gctatagatg cagtggttcg aaaaaaactg gatggttacg atcccgaaag cggagttgcc
     3001 ggcctggtca tggccactgt gcttgacacg gctcagttga acaaagcgat gcggccactt
     3061 agctccgctt taatacaacc tgtgtacaaa caagaaaata cctcggccct ggtgatgcat
     3121 attcccagcg gctggccctt gtacggcttc gataaaaggg agcttacaca ggataggaat
     3181 ctgtatgggg agtttttcga aatcacccac cggcaaaaat gagttttttt attgtataac
     3241 gatagatagt aagattatga gcctgaaaat tgaaaaaata ccttttggta gcatgcccga
     3301 cggcagaacg gtgtgggtgt tcttccgcgt gaacaacacg ttaggtg
//
LOCUS       DAOSGL010000257         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_26846_length_3323_cov_2.928091, whole genome shotgun sequence.
ACCESSION   DAOSGL010000257 DAOSGL010000000
VERSION     DAOSGL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26846_length_3323_cov_2.928091"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PKY68_07715"
     CDS             <1..673
                     /locus_tag="PKY68_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24954.1"
                     /translation="FSSDGINLSTQHAGEQLEESQQGLFIAPDGQILYSGMVNNALAV
                     AGNQQPVAFASEATLDIPALFKAESAKFVNQKTDPAMAGLFAACKMVKDMGISLTGKS
                     VQETLDKNNPGFKTSCPNIYKNFGSIDFVRNAQGIITILTVGKKNISFDKVRISNNST
                     ISITEIPGNNYRVDALSGIKVGMAVVWFDLNFIKLMASNGDMVFDYSSDHSQVSVNLQ
                     KDILE"
     gene            714..1280
                     /locus_tag="PKY68_07720"
     CDS             714..1280
                     /locus_tag="PKY68_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24955.1"
                     /translation="MKTSILKVLGLGVIAGMMFYSCGSSKKITPKNDGEIEIVSYCSG
                     PEYQSTNKAFRYTGIGESMNQMTAKNMAMSQARAGLAATINTTIKTVTDNYVKSGNFN
                     NKEELLNNYEGMTREVVNQTLSGAVVICEKMTKTQQGNYKSYICMEYGASDVLQNINN
                     RATSQEILKVDYNYEKFKSTFEEEMSKF"
     gene            1412..3079
                     /locus_tag="PKY68_07725"
     CDS             1412..3079
                     /locus_tag="PKY68_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24956.1"
                     /translation="MKTRYSYIFLLLLTACLSPAQGQDQGEMLPEERYTRNSASIIRM
                     EYGDSFDARVAAHINNTSLGGRFDINEIALQALKISESRNYYPMGSSLPTAPDRTELI
                     STMLNEQQAGQQIVAYVFNRQSDGNMNLDRVFERGEYSAHDVDMRLSGTLKVETVRDA
                     GSQLIGRSYLMVYDVTNLRQEYSGEGDSRELYWKATAALYLYKLNWDEQDLDTFYDKY
                     WLDDNTPAEEKQAKKAAFEAWNIPVELISRISLFDKSVSTGIEANKRLGVLGDKKKTD
                     EQRLSDAFAELHKQICSDLNYKIEKQHEDFVVTTPITSLRPIRAKIGTKEDVKHGLRF
                     YAYGFKGNSDGSIKLVRKGILRATKNVADNRIVATGESPESEFTKIAGYGRVVEGLTL
                     VEKKDLNLSLGVGFNSIPQGGPVKGGFHLDLSYENHTRKGFCAYATATLNLDGDAMNY
                     LLSYGYGLRIPNFEIYPFAGVGIDAYSFDGDDAYESSDLNAYLVQGGLRAQLNIFYPV
                     YLFWNVSFNTVFGKGYKYEELEGTSDSNGNYRDMNGTQTALGVRYCF"
     gene            3120..>3323
                     /locus_tag="PKY68_07730"
     CDS             3120..>3323
                     /locus_tag="PKY68_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24957.1"
                     /translation="MKTKYLFAVMLTALFCLAVFRLEAQSARAVNKGMFKSAASYEVQ
                     VLGVGQDGTKVFRIWAYDKTVEAA"
BASE COUNT          984 a          821 c          760 g          758 t
ORIGIN      
        1 attctcgtcc gacggtatca atttgtccac ccaacacgcc ggagaacaac tagaagaatc
       61 gcagcagggc ctgtttattg ctcccgacgg acaaattctg tattcgggca tggtcaacaa
      121 cgccctggca gtggcaggca atcagcagcc ggtagccttc gcatcggagg ctacacttga
      181 tattcccgct ttgttcaaag ctgaatcggc caaatttgta aatcaaaaaa ccgatcccgc
      241 catggccggt ctgttcgccg cctgcaaaat ggtaaaagac atgggcatca gcctcaccgg
      301 caagtctgta caagaaactc tcgacaaaaa caacccgggt ttcaaaacat cttgccccaa
      361 tatttataaa aatttcggat ccatcgattt cgtccgcaac gctcagggca ttataaccat
      421 cctgactgtg ggtaaaaaga acatttcctt cgacaaagtc aggatctcca acaacagtac
      481 catttccatt actgagatcc cgggcaacaa ttaccgtgtt gatgcgcttt cgggcatcaa
      541 agtaggaatg gctgtagtat ggttcgattt gaattttata aaactgatgg cctccaacgg
      601 agatatggtc tttgattact catccgacca ctcacaggtt tcagtcaatt tacaaaaaga
      661 catcttagaa taattgcaat cacttttttt atcaaccatt aaattaaaaa attatgaaaa
      721 cctcaatctt gaaagtatta ggactcggtg ttattgccgg catgatgttt tattcctgcg
      781 gttcctccaa aaaaattaca cccaaaaacg atggcgaaat cgaaattgtt tcttattgct
      841 ccggtcccga ataccaaagc accaacaagg ccttccgtta tacgggcatc ggcgaaagca
      901 tgaaccagat gaccgccaag aacatggcca tgagccaggc tcgtgccgga ttggccgcca
      961 ccatcaacac caccatcaag actgttaccg acaattatgt aaaatcaggg aatttcaaca
     1021 acaaagaaga attactgaac aactacgaag gtatgacccg cgaagtagtc aaccaaaccc
     1081 tgagcggagc cgttgtaatt tgcgaaaaga tgaccaaaac ccagcaaggc aactacaaat
     1141 cctatatttg catggaatat ggtgccagcg atgtattgca aaacatcaac aaccgtgcta
     1201 ccagccagga aattctgaaa gttgactaca actacgagaa attcaaaagc accttcgaag
     1261 aggaaatgag caaattctga ttttttctta cttcgacctc taaacaattg aattcagagg
     1321 atatactcag ttgacgggca ctaaacagcc tgccaactga gttgtatatc aaacagctat
     1381 ccaaaccaaa attgttcagg aaccccctac tatgaaaacc agatacagtt atatattttt
     1441 gctgctgctc acagcatgcc tgtcaccggc ccaaggccag gaccagggag agatgcttcc
     1501 cgaagagcgc tatacccgca attcggccag tattatccgt atggaatacg gggattcttt
     1561 cgatgccagg gtcgctgctc acatcaacaa caccagtttg ggcggccgct tcgacatcaa
     1621 tgaaattgca ttgcaggctt tgaagattag tgaatcgcgt aattactacc ccatgggtag
     1681 cagcctgccc acagcacccg accgcactga acttatcagc accatgttga atgaacaaca
     1741 ggcgggccaa caaattgtgg cttatgtttt caaccggcag tcggacggca acatgaatct
     1801 ggaccgggtg tttgaacggg gggaatacag cgcccacgac gtggatatgc gcctgagcgg
     1861 caccctcaag gtggagaccg tccgcgatgc cggcagccag ctgatcggcc ggagttatct
     1921 gatggtctac gatgtcacca acctgcgcca ggaatattcg ggtgagggcg acagccggga
     1981 actctattgg aaagccactg ctgccctgta tttatacaag ctcaactggg acgaacaaga
     2041 cctggatact ttctacgata agtactggct ggacgacaat acgccggccg aagaaaaaca
     2101 agccaaaaaa gccgcctttg aagcctggaa cataccggtg gaattaatca gccggatcag
     2161 cctcttcgat aaaagcgttt ccacgggcat agaggccaat aaaaggctgg gtgtgctggg
     2221 agacaagaaa aaaacagacg aacaacgcct ttcggacgct ttcgccgaac tgcacaaaca
     2281 aatttgcagc gacctgaatt ataaaattga gaaacagcac gaagacttcg tggtcactac
     2341 tcccattacc agcctgcgac ccatcagagc caaaatcggc accaaagaag acgtcaaaca
     2401 cggcctgcgg ttctatgcct acggcttcaa gggtaacagc gacggcagca tcaaactggt
     2461 acgcaaaggt attttacggg ccaccaaaaa cgtggcagac aaccgcatag tagctaccgg
     2521 cgaaagccct gaatcggaat tcaccaaaat agccggttac ggaagggtgg tcgaagggct
     2581 taccctggta gagaaaaaag acctgaatct ttcacttgga gtgggattca acagtattcc
     2641 gcaaggagga cccgtcaaag gaggatttca cctggatctt tcttacgaaa accacacccg
     2701 aaaaggattc tgtgcatatg ctacagccac tctcaacctt gatggcgacg ccatgaatta
     2761 ccttttgagt tacggttacg gcctgcgcat acccaatttt gaaatctatc cttttgccgg
     2821 tgtcggtata gatgcctaca gttttgatgg cgacgacgct tacgaaagtt ccgatttgaa
     2881 cgcctacttg gtgcaaggcg gtttacgggc acagctcaat atcttttatc ccgtatacct
     2941 gttctggaat gtgagcttca atacggtttt tggtaaagga tacaaatacg aagaattgga
     3001 aggcacatcc gacagcaacg gcaattaccg cgatatgaac gggactcaga cagcgctggg
     3061 tgtccgctac tgtttttaaa tttacctttt aaaatatcca tcattagacc aacaagatta
     3121 tgaaaaccaa gtatctattt gctgtgatgt tgaccgctct gttttgtctg gccgtattcc
     3181 ggctggaggc tcaaagcgcc cgggctgtca acaaaggaat gtttaagagc gctgccagtt
     3241 acgaagttca ggtgttggga gttggccagg atggcaccaa ggtgttcagg atctgggctt
     3301 acgacaagac agtggaagcg gcc
//
LOCUS       DAOSGL010000258         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_27008_length_3308_cov_3.150015, whole genome shotgun sequence.
ACCESSION   DAOSGL010000258 DAOSGL010000000
VERSION     DAOSGL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27008_length_3308_cov_3.150015"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1767
                     /gene="purH"
                     /locus_tag="PKY68_07735"
     CDS             244..1767
                     /gene="purH"
                     /locus_tag="PKY68_07735"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOH24958.1"
                     /translation="MSDNKSMRTALISVYHKEGLDEILHQLHREGVEFISTGGTQKFI
                     ESLGYPCEKVESLTGYPSVLGGRVKTLHPKIFGGILNRRSLDTDRQECVEYDIPAIDL
                     VIVDLYPFQETVKSGAAAEDIIEKIDIGGISLIRAAAKNFNDVVIVASQAQYPALLDL
                     LNEQGATTSLEDRRWFAREAFAVSSGYDAAIFNWFDGADGSALRVAIDHSKVLRYGEN
                     PHQQASFFGDFDELFEQLNGKEISYNNLLDIDAAVSMVNDFDETTFVIVKHNNACGLA
                     SRPVLVDAWNAALSADPVSAFGGVFATNAAIDKATAEEINKLFFEVLIAPDYDFDALE
                     ILTQKKNRILLIQKEIPQGGKQFRSLFNGVLVQDKDTLTETAEDLRCVTEKESDAAQI
                     EDLLFANKVVKHNKSNAIVLVKDKQLCAAGVGQTSRVDALRQAIDKARSFDMELEGAV
                     MASDAFFPFPDCVEIADEAGITAVIHPGGSINDKLSIEYCNEHGMAMYTTGYRHFKH"
     gene            1775..2794
                     /locus_tag="PKY68_07740"
     CDS             1775..2794
                     /locus_tag="PKY68_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOH24959.1"
                     /translation="MGIFSFTQKIAIDLGTANTIIMCNDKIVVDEPSVVALDRKTDKM
                     LAVGEKARLMHEKTHENIRTVRPLRDGVIADFYAAEQMIRGLIKIALPRGSGFFSSLR
                     MVVCIPSGSTEVELRAVRDSAEHVGGREVYMIYEPMAAAIGIGIDVEAPEGNMIVDIG
                     GGTTEIAIISLGGIVANKSTRIAGDDFTTDIQEYMRRQHNLRIGERTAERIKINVGSA
                     LTELENPPEDFLVYGPNQMTALPMEVPVSYVEIAHCLEKSVSKVETAILNALEQTPPE
                     LYADIVRNGIYLTGGGSLLRGLAKRLSDKINIPFIVPEDPLHAVARGTSVTLKNVDRF
                     NFLMR"
     gene            2845..>3308
                     /gene="mreC"
                     /locus_tag="PKY68_07745"
     CDS             2845..>3308
                     /gene="mreC"
                     /locus_tag="PKY68_07745"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOH24960.1"
                     /translation="MFLEILSLILLFRFNDYQQGVLFSSANRINGKLYTFREAVSGFF
                     ALRENNTELLRINSEMELKIARLEEGLRKAGLEEEQLKAAGSYEFIHARVIKNSVSQL
                     RNYLTLNVGKLDGVAEGMGVCNHLGIVGVVSVCSDHFSVVIPVLNPDISFSC"
BASE COUNT          882 a          762 c          804 g          860 t
ORIGIN      
        1 tgccgtacag acgtactgct gttgttttca tcttggtttt cgagtaaaaa attaatactc
       61 gcaaaggtag taaaaatcct caagctctct ttaatggaaa taatataaaa aagccttttt
      121 aaaacacttg tttttattta catttgcaaa tcttatcggg cagcgctctg attggccttt
      181 atccgcttaa gaaagagcaa caaagcctgt tttttttgga tcgaaatttt catttttatt
      241 cctatgtcag acaataaaag tatgcggaca gccctgattt ctgtttatca caaagaaggt
      301 ttggacgaaa ttttacacca attgcaccgg gaaggggttg aatttatctc caccggaggc
      361 acccaaaaat ttattgaatc actgggttat ccctgcgaaa aagttgaaag cctcaccggt
      421 tatccttcgg tactgggcgg tagagtaaaa accctgcatc ccaagatatt tggcggcatt
      481 ctcaaccgcc gctcgctgga taccgaccgg caggaatgcg tagaatacga tatacctgcc
      541 atcgacctgg tgatagtgga tttgtatccc tttcaagaaa cagtaaaatc gggagccgct
      601 gccgaagata ttatagagaa aatagatata ggcggcattt cgcttatcag ggcggctgcc
      661 aagaacttca acgacgtggt tattgttgct tcacaagctc aataccctgc tttactcgac
      721 ttgctgaacg agcagggagc taccacttca ctcgaggacc gtcgttggtt tgcccgtgaa
      781 gcttttgccg tttcatcggg ttacgatgcc gccattttca actggttcga cggggcagat
      841 ggctcagctc tgagagttgc catcgaccac tccaaggttt tgcgctacgg cgagaatccc
      901 caccagcaag cttccttttt cggtgatttc gatgagttgt ttgagcagct caacggtaag
      961 gaaatttctt acaacaatct attggatata gacgcagccg tatctatggt aaacgatttc
     1021 gacgaaacca cttttgttat cgtcaaacac aacaacgcct gcggactggc gtcgcgtcct
     1081 gtgctcgtag atgcctggaa tgccgctctt tcagcagatc cggtgtcggc ttttggaggc
     1141 gtttttgcca cgaacgccgc catcgacaaa gcaacggccg aagaaatcaa caaacttttc
     1201 ttcgaagtat tgattgcgcc cgactacgat ttcgacgctt tagaaatatt gacccaaaag
     1261 aagaatcgca ttcttttgat tcagaaagaa attcctcaag ggggaaaaca atttcgctcc
     1321 ctgttcaacg gagtgttggt gcaggataag gatacgctta ccgaaaccgc agaggatttg
     1381 cgttgcgtta ccgaaaaaga gagcgatgca gctcagatcg aagacctgct ttttgccaac
     1441 aaggtggtga agcacaataa gagtaacgct atcgtgttgg taaaagacaa acagctttgt
     1501 gcggccggcg tgggtcaaac ctccagggtg gatgccctaa ggcaggccat cgacaaagcc
     1561 aggagcttcg acatggagct cgaaggggcc gtaatggctt ccgatgcctt tttccctttc
     1621 cccgattgcg tcgaaattgc agatgaagca ggcattacgg ctgtaattca tcccggggga
     1681 tccatcaacg acaaattatc catcgaatat tgcaacgaac acggtatggc catgtacaca
     1741 accggataca gacactttaa acattaaaac cgatatggga atcttttctt ttactcaaaa
     1801 aatagccatc gatttgggca ctgccaacac tatcattatg tgtaacgata aaattgtggt
     1861 agacgaaccc agtgtagtag ccctcgatcg taaaaccgac aagatgttgg ccgtaggcga
     1921 gaaagcacgg ctgatgcacg aaaaaactca tgaaaatatc cgcaccgtgc gtccattgcg
     1981 cgacggagtc atagccgact tttatgctgc cgagcaaatg atccgcggac tgattaagat
     2041 cgctctgccc cggggcagcg gtttcttttc ttctctgcgt atggtggttt gcattccttc
     2101 gggctcaacc gaagtggaac tgcgtgccgt gcgcgactcg gccgagcacg taggcggacg
     2161 cgaagtgtat atgatatatg aacccatggc agccgccatt ggtataggga tcgatgtgga
     2221 agcccccgaa ggcaacatga tagtggacat aggcggcggc actaccgaga ttgccattat
     2281 ttcgctgggc ggcatagtag ccaacaaatc cacccgcatt gccggtgacg attttaccac
     2341 agacattcag gaatatatgc gccggcaaca caacctgcgt ataggggaac gtacggcaga
     2401 acgcattaaa atcaatgtag gctctgctct aacggagctg gaaaatcctc cggaggactt
     2461 tttggtatac ggccccaatc aaatgacggc tttgccaatg gaagttcccg tttcctatgt
     2521 ggaaattgcc cattgccttg aaaagtcggt ttccaaagta gagaccgcga ttttaaatgc
     2581 tttggagcaa actcctccgg agctttatgc cgacatcgta cgtaacggca tctacctgac
     2641 cggtggagga tctttgctca gaggtttggc caaacgcctg agcgataaga tcaatattcc
     2701 ctttatcgtg cccgaagatc ctttacatgc tgtagccaga ggtaccagcg taactttgaa
     2761 gaacgtagat agattcaatt tcctgatgcg ttaatgcgac aacttattca ttttctcgtc
     2821 cggtacagaa acttcttctt gtttttgttc ctggaaatcc tgtcgctgat actcttattt
     2881 cgttttaacg actatcagca aggggtttta ttcagttcag ccaatcgaat aaacgggaaa
     2941 ttgtatactt tcagggaagc ggtaagcggt ttttttgccc tgcgcgaaaa caacacggag
     3001 ttgttacgca tcaacagtga gatggaactc aaaatagctc gtctcgaaga gggtttgcgc
     3061 aaagccgggt tggaagagga gcaattaaaa gccgcgggca gctacgaatt cattcatgcc
     3121 agggttatta aaaacagcgt ttcgcaactg cgaaactacc tgaccctaaa tgtgggtaag
     3181 ctggacggag ttgccgaagg catgggggtt tgcaatcatc tgggtatagt gggggtggtg
     3241 agtgtctgct ccgaccattt ttcggtcgtt attccggtgc tgaacccgga tatcagtttc
     3301 agttgtat
//
LOCUS       DAOSGL010000259         3290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_27234_length_3290_cov_3.914065, whole genome shotgun sequence.
ACCESSION   DAOSGL010000259 DAOSGL010000000
VERSION     DAOSGL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27234_length_3290_cov_3.914065"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1992)
                     /gene="glgP"
                     /locus_tag="PKY68_07750"
     CDS             complement(<1..1992)
                     /gene="glgP"
                     /locus_tag="PKY68_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOH24961.1"
                     /translation="MKTKHCSNQPQWSSVTVQSNMPAGLGKIQELVRNLWWSWNDEII
                     RLFREIDRPLWVQVSGNPVAFLESLSYTQLKQIAEDKAIMQRLDSIYAKFREYMDVPV
                     NKEAPSVAYFSMEYGLTDVLKIYSGGLGVLAGDYLKEASDCNIPMTAVGFLYRNGYFT
                     QGLSMEGQQIAIYEPQNFNTLPIEQVKDEQGNPLVLKVDFPGYIVNCYIWKAAVGRVD
                     LYLIDTDHPGNSEFERQITAQLYGGDWENRLKQEYLLGIGGIQLLNILGIKKQVYHCN
                     EGHAALINAQRMVDLIAQEGLNFSEALEVVRASSLYTVHTPVPAGHDKFDEDLMFKYM
                     SSFPQKLGISWARFMDMGRENEGDTNEKFSMSVFACNCCQEVNGVSMLHGKVSQKMFS
                     NIWKGYFPDELHVSYVTNGVHMPTWTASEWRSLYEKTFGPQWMGDQSNIELWSKIYEV
                     SDEEIWKTRQQLKLKLIEYIRNKYRDSWFKNQGDPSRIMAILEKINPNTLMIGFGRRF
                     ATYKRAHLLFTDLERLSRIVNNPLYPVQFVFTGKAHPADGGGQGLIKRIVEISRMPEF
                     QGKIIFLENYDMALAKRLISGVDIWLNTPTRPLEASGTSGQKAEMNGVLNFSVLDGWW
                     LEGYVEKAGWALTEKRTYESQEFQDQLDAATIYSMLENEI"
     gene            complement(1994..>3290)
                     /locus_tag="PKY68_07755"
     CDS             complement(1994..>3290)
                     /locus_tag="PKY68_07755"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOH24962.1"
                     /translation="EQDPAVVAHFHEWTTGLGLLYVKKHLPCVATVFTTHATSIGRSI
                     SGNGKKLYKYIEGYQGDQMARELNMVAKHSVEKTAAFQADCFTTVSDITAAECAQLIG
                     KAPDLVTPNGFEDDFLPRGGVAIAKRKAARRLLLEVAESILGYSLNKNVLMLATSGRY
                     EFRNKGLDVFIESLHRLRHDRSLDRQILAYILVPGDVSAARQEVLQRLEALRKSKTTS
                     SSAKQQSDLGNSPEAESAQPGPIYHPYNTHWLNHIEQDKVLNTIKHLGFTNGPDEKVK
                     IIFVPCYLHGHDGIFNKSYYDILQAFDLTAFLSYYEPWGYTPLESLAFSVPTLTTSLS
                     GFGRWLQKLGYKDTDIRQGAAVIPRNDSNYEEVVQQTAGVFADFASLHSEERKEVRKK
                     AGRLSKQALWSNFIKYYQQAYDLALSKVKKYNKQKKTSK"
BASE COUNT          736 a          798 c          854 g          902 t
ORIGIN      
        1 aatctcgttt tcgagcatgg agtaaatggt agctgcatcg agctgatcct ggaactcctg
       61 gctttcatag gtacgttttt cggtcagagc ccagccggct ttttctacat aaccttcgag
      121 ccaccacccg tcgagcaccg agaaattgag cacgccgttc atttcggctt tttggcccga
      181 ggttcccgaa gcttccaagg gacgggtcgg ggtattgagc caaatgtcca cacccgaaat
      241 caggcgtttg gccaaggcca tatcgtaatt ctcgaggaag atgatcttgc cctggaattc
      301 gggcatgcgc gaaatttcca cgatgcgctt aatcagtccc tgtccgcctc cgtcggccgg
      361 gtgggccttg ccggtaaaga caaattgcac gggataaagg ggattgttca ctatgcggga
      421 caaacgttcc aggtcggtaa acaacaaatg ggcacgtttg taggtggcaa aacggcgacc
      481 gaaacctatc atcagggtat tgggattaat tttttccaat atggccatga tgcgcgaggg
      541 atcgccctgg tttttgaacc aactgtctct gtatttgttg cgtatgtact caatcaattt
      601 aagcttgagt tgctgacggg ttttccaaat ctcttcgtcg ctgacttcgt agattttgct
      661 ccagagttca atattggact gatcgcccat ccattgggga ccgaaagttt tttcgtagag
      721 actgcgccat tcgctggcgg tccaggtggg catatgaacg ccgttggtaa cataacttac
      781 atgtaactcg tcggggaaat aacctttcca gatattgctg aacatttttt gggacacctt
      841 gccgtggagc atgctcacgc cattgacctc ctgacagcag ttgcaggcaa aaacgctcat
      901 gctgaatttt tcgttggtat cgccttcgtt ttcgcggccc atatccataa atctggccca
      961 actgatgccc aatttttggg gaaagctgct catgtatttg aacatgaggt cttcgtcgaa
     1021 tttatcgtga ccggcgggaa ccggggtatg taccgtgtag agcgaagagg cgcgtactac
     1081 ttcgagggct tcgctaaagt taagtccttc ctgagcaatc aggtcgacca tacgttgagc
     1141 gttgatcagg gcggcgtggc cttcgttgca atgatatacc tgttttttga tacctaggat
     1201 attgagcaac tgtatgccgc caatgcccag cagatattcc tgtttcaagc gattttccca
     1261 gtcgccgccg tagagttggg cagtaatttg acgctcaaac tcgctgttgc ccggatggtc
     1321 ggtatctatc aggtagagat ccactctgcc cacggctgct ttccagatat agcaattcac
     1381 tatatagccc gggaagtcca ctttcagcac caagggattg ccttgttcgt ctttgacttg
     1441 ttctatgggc agtgtattga agttttgagg ttcatagatg gcaatttgct gaccttccat
     1501 cgaaagtccc tgagtaaaat aaccgttgcg gtagagaaaa cccacggccg tcatgggaat
     1561 attgcagtct gaagcttctt tcaggtaatc gccggccaat acgcccaagc cgcccgaata
     1621 gatcttgagc acgtcggtca aaccgtattc catgctgaaa taagccaccg aaggagcctc
     1681 tttgttgaca gggacatcca tgtactcgcg gaattttgcg taaatgctgt ctaagcgctg
     1741 catgattgcc ttgtcttcgg ctatttgttt cagttgggta taagaaaggc tttccaaaaa
     1801 agcgacggga ttgcccgaaa cctgcaccca gaggggtctg tctatttctc taaataatct
     1861 gatgatttcg tcgttccagc tccaccaaag attacggacc aattcctgta ttttcccgag
     1921 tccggcaggc atgttggatt gtacggtcac actggaccat tggggctgat tactgcaatg
     1981 ctttgttttc atattattta cttgtttttt tctgtttatt gtacttcttg accttggaca
     2041 aagccaggtc gtatgcttgt tggtaatact ttataaagtt cgaccacaac gcctgtttag
     2101 acagacgtcc ggcttttttt cgcacttctt tgcgctcttc gctgtgcagg gaggcaaaat
     2161 cggcaaatac tccggccgtt tgctgaacga cttcttcgta attactgtcg ttgcggggaa
     2221 taacggccgc accctgcctg atgtccgtgt ctttgtagcc cagtttctga agccaacggc
     2281 cgaaacccga cagcgaagtg gtgagcgtgg gcaccgaaaa ggccaggctt tcgagcgggg
     2341 tataacccca aggttcgtaa tacgacaaaa atgccgtcag gtcgaaagcc tgtagaatat
     2401 cgtaatagga tttgttgaat atgccgtcgt gtccgtgcag gtagcagggc acaaagataa
     2461 ttttgacttt ctcgtccgga ccattggtaa agcccagatg tttaatggtg ttgagcactt
     2521 tatcctgctc aatgtggttg agccagtgcg tgttgtaggg atgataaata gggccgggtt
     2581 gagctgactc tgcctccggg gaatttccca aatctgattg ctgtttggcc gaagacgaag
     2641 ttgttttgct ctttctgagc gcctccaacc tttggagcac ttcctgacgg gcagcgctta
     2701 catcgccggg caccaggata taagccagaa tctgccggtc gaggctccgg tcgtgacgca
     2761 gccggtgcaa gctttcgata aagacgtcca gccccttgtt gcgaaactcg taacgtccgc
     2821 tggtagccag catcaacacg tttttattga gactgtaacc cagtatgctt tcggccacct
     2881 cgagtaaaag ccggcgggct gctttgcgtt tggcaatggc cacgcctccc cggggcagaa
     2941 aatcgtcttc gaaaccgttg ggggtgacca gatcgggggc tttgccgatt agttgagcac
     3001 attcggcggc tgtgatgtcg ctgacggtgg taaagcaatc ggcctgaaag gctgccgttt
     3061 tttcgaccga atgtttggcg accatattga gctcccgggc catctgatcg ccctgatagc
     3121 cctctatgta cttatagagt tttttcccgt tgccactaat ggaacggcct atggaagtgg
     3181 catgggtagt aaatacggtg gccacacagg gcaggtgttt tttgacgtag agcaaaccca
     3241 gcccggtggt ccattcatgg aaatgggcta ctactgcagg atcttgttcc
//
LOCUS       DAOSGL010000260         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_27513_length_3267_cov_3.933686, whole genome shotgun sequence.
ACCESSION   DAOSGL010000260 DAOSGL010000000
VERSION     DAOSGL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27513_length_3267_cov_3.933686"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PKY68_07760"
     CDS             complement(<1..659)
                     /locus_tag="PKY68_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOH24963.1"
                     /translation="MKSRYIGIAFTTILMIFGMELAAADEVVFTATAPSKVILGQQFR
                     LTYTLNAETKDLRIPEITDFEVLMGPSTAKSTKISIVNGQRTSLVEHSYTYVLMPVKV
                     GSFTIPAATATVKKEKRSSNPLTITVLPQDKSTGGASDQAASSQQTASFNGEQAFIRA
                     IPSKTRVFEQEGLLLTYKLYTRIDISGVNNIKFPEFKGFLAQEIEIDPNTQWDMENYE
                     GL"
     gene            complement(706..1416)
                     /locus_tag="PKY68_07765"
     CDS             complement(706..1416)
                     /locus_tag="PKY68_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH24964.1"
                     /translation="MRSIGIFMFLFLFSLAGFAQKAERKHIRQGNSLYEEEKFTEAEI
                     EYRKSLEVDRQSPEGIFNLGNSLFKQEKPKEAIEQYQQITGTEKDSMRLSSAWHNIGN
                     SFMQAQDFGKAIEAYKQALIHNYKDDQTRYNLAMAQAMLKKQQEQPQQEQPQQEQQQQ
                     EEEQKQEQQEQQEQQEQERPEQQNQPDQQEQQQEHISREKAQQLLDALMQDEQEVQEK
                     VKKLQIQNSRPKKTEKDW"
     gene            complement(1418..2431)
                     /locus_tag="PKY68_07770"
     CDS             complement(1418..2431)
                     /locus_tag="PKY68_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH24965.1"
                     /translation="MFRFSDPNYFYLLILPLLLFGLFLLMNRRSLKQLCKYGNPRLLK
                     PLMPEVSTARRWLHLLLQLTAMLVTVFMLAGPQFGSKLEKQKRQGAEVIVALDVSNSM
                     MAEDLVPDRLEKSKQTLSRLIDQLANDRIGLIVFAGEAYTQLPITADYISSKMFLNTI
                     STEMVPTQGTAIGAAIQLALRSFGLAREAERAIVVITDGENHEDDAVLQAQEAQKQGV
                     KVHVVGMGSLKGAPIPRGLNDFHKDKEGNVVITKLNEEMCQQIAMAGGGVYVRADNTN
                     TALRVLSKELDSMKKSDIETTVYAEYDEQFQALAWIVLLLLFVDMFILDKKNPALRNL
                     KLF"
     gene            complement(2444..>3267)
                     /locus_tag="PKY68_07775"
     CDS             complement(2444..>3267)
                     /locus_tag="PKY68_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH24966.1"
                     /translation="LPIVLRSLAFTLLVIVLARPQSVDSWRNTTTEGIDIVLALDIST
                     SMLAEDLKPNRLEAAKSIASSFIAGRDNDNIGLVVFAAESFTQCPLTTDHAVLMNLFG
                     SIQSGMIEDGTAIGLGLANAVSRIKESQAKSKVIILLTDGTNNRGEIDPITAAELAKT
                     FGIRVYTIGVGTIGKAPYPFRTANGVVYQNIDVVIDEEPLKQIAGMTGGVYFRATDNT
                     KLQGIYQEIDQLEKTKIEVQEYSKREEEYRPFALWALFLLVLEFSLRLFVLRSLP"
BASE COUNT          816 a          774 c          689 g          988 t
ORIGIN      
        1 agaccctcgt aattttccat atcccactga gtattgggat ctatttcaat ttcttgggcc
       61 aaaaagcctt tgaactcagg aaatttaata ttgttcactc cgcttatatc tattcgggta
      121 tacaatttat aagtcaacaa caaaccttct tgttcaaaga ccctggtttt ggaagggatc
      181 gctctgataa atgcctgttc gccattgaaa ctggctgttt gttgcgagga agctgcctgg
      241 tctgaagctc cgcctgtgct tttatcctga ggaaggaccg taatagtaag cggattggag
      301 ctcctttttt ctttttttac ggtcgcagtg gccgcaggaa tagtaaaaga ccccactttt
      361 acgggcatca aaacataggt atagctgtgt tcgaccagag aagtccgctg accgttgact
      421 atgcttatct tggtagattt cgcggtacta ggtcccatca atacttcaaa atcggtgatt
      481 tcgggtattc tgaggtcttt ggtttcggca ttcagggtgt aagtaagcct aaactgttga
      541 cccagaatga cctttgaagg agctgttgcc gtaaaaacaa cttcatcggc agccgccagc
      601 tccataccga aaatcatcaa tatggttgta aacgctattc ctatatatct acttttcata
      661 catacaccgt aatcttaccg cactcaggaa acaaaggtag tctttttacc aatccttttc
      721 tgttttcttc ggccggctat tctgtatttg taatttcttc actttttcct gtacctcctg
      781 ctcgtcctgc atcagggcat ccaacaattg ctgcgccttt tcacggctta tgtgttcttg
      841 ctgttgttct tgctgatcgg gctgattttg ctgctcgggt ctttcctgtt cctgctgttc
      901 ctgctgctct tgttgttcct gcttttgctc ttcttcttgt tgctgttgtt cctgctgtgg
      961 ttgttcctgc tgtggttgtt cctgctgttt ttttaacata gcttgagcca tggccagatt
     1021 atagcgggtc tgatcgtcct tataattatg aatcaatgcc tgcttgtaag cctcaatggc
     1081 tttcccgaaa tcctgagcct gcataaaaga attgcctata ttatgccagg cagacgacaa
     1141 tcgcatagaa tctttctcgg tgccggtaat ttgctggtac tgctctatgg cttctttagg
     1201 cttttcttgc ttaaacagag agtttcccaa attgaagatg ccttcgggcg attgcctgtc
     1261 cacctccaaa gatttgcgat attcaatttc ggcttcggtg aatttttcct cttcataaag
     1321 actattcccc tggcgaatat gtttcctttc tgctttttga gcaaaaccag ccaggctgaa
     1381 caaaaacaag aacataaata taccgatgct tcgcatatca aaagagtttt aaattacgta
     1441 aagccggatt ttttttatcc agaataaaca tatccacaaa caaaagcaat aaaactatcc
     1501 aggccagagc ttgaaattgc tcatcgtatt cagcgtaaac ggtggtttcg atatcggact
     1561 ttttcatcga gtcgagctct ttactcaaca ccctgagcgc cgtgttggtg ttgtcggcac
     1621 gaacatatac ccctccaccg gccattgcta tttgctgaca catttcttcg ttgagtttag
     1681 taatgaccac attaccctct ttgtctttgt ggaagtcgtt caaaccacgg ggtatagggg
     1741 ctcccttgag agatcccata ccaaccacat gaactttaac accctgtttt tgagcttcct
     1801 gagcctgcaa aacggcatcg tcttcgtggt tttcaccgtc ggtaattacc acgatggctc
     1861 gttccgcttc ccgggctaac ccaaaagaac gtaaagccag ctgaatggct gctcctatgg
     1921 cagtgccctg agttggaacc atctccgtag aaatagtatt caaaaacatt ttggacgaaa
     1981 tgtaatcggc tgtaatgggt aattgagtat aagcctctcc ggcaaaaaca atcagaccaa
     2041 tcctatcgtt agccaattga tctatcaacc tggaaagagt ttgttttgac ttttcgaggc
     2101 gatcgggtac taaatcttcg gccatcattg aattggaaac atccagtgca acaataacct
     2161 cggccccttg tctcttttgt ttttccaatt tggagccaaa ctgaggcccg gccaacatga
     2221 atacagttac aagcatggcg gtcaattgta ggaggagatg cagccaacgc cgggctgtag
     2281 aaacttcagg cattaagggt ttgagcaacc gcggatttcc gtacttgcag agttgtttga
     2341 gactcctgcg gttcatcagc agaaaaagcc cgaataacaa cagcggcagt atgagcaaat
     2401 agaagtagtt tggatcggag aatctaaaca taacatttaa attttagggc aagctgcgca
     2461 aaacgaataa acgtaaggaa aactctagta cgagcaggaa cagtgcccac agagcaaatg
     2521 gtctgtattc ctcctcgcgt ttactgtatt cctgtacttc tattttggtt ttttccaatt
     2581 gatcaatttc ctgatagatt ccctgcaatt tggtgttgtc cgtagcccgg aaatatactc
     2641 ctccggtcat gccggcaatt tgtttgagag gttcctcatc aatgaccaca tcaatatttt
     2701 gatataccac tccgttggcg gttctgaaag gataaggagc tttgcctatg gtccctaccc
     2761 ctatggtgta tacccgtatt ccgaaggttt tggccaattc tgccgctgtg atcggatcga
     2821 tttcgccccg gttgttggtg ccatcggtca acaaaataat aaccttggat ttagcctgag
     2881 attctttgat cctgcttacg gcatttgcca atcccaatcc tatagccgta ccgtcttcta
     2941 tcatgccgct ttgaatgctc ccaaagaggt tcatcaacac agcatgatcc gtagtcaagg
     3001 gacattgagt aaaactttcg gcggcaaaca caaccaggcc gatgttgtcg ttgtccctgc
     3061 ccgctataaa cgaagaagca atacttttgg ccgcctccag gcgattgggc ttaaggtctt
     3121 cggccagcat actggtcgaa atatccagtg ccagtacaat atcgatacct tccgtagtag
     3181 tattccgcca actgtctacc gattgagggc gggccaatac tataaccaac agagtaaaag
     3241 ccaggctgcg caaaactata ggtaaat
//
LOCUS       DAOSGL010000261         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_27539_length_3264_cov_4.781552, whole genome shotgun sequence.
ACCESSION   DAOSGL010000261 DAOSGL010000000
VERSION     DAOSGL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27539_length_3264_cov_4.781552"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKY68_07780"
     CDS             <1..651
                     /locus_tag="PKY68_07780"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH24967.1"
                     /translation="SRNVSSDVIDVDDLILGDRSITNTYNAGENLSLTFTHTIIEAGF
                     RGRFNYSRSQNNLNPDRIQTTINWSANGNVTLHLPYDINIDNDLSYSARQGYANFDQN
                     ELLWNARIDKAVFKKRFTLQLRINDILRQRQNINQSIGDNSVSYNKSDMLTAYYIVSL
                     TYRIRQFGGRAPGSGRGGGGGFAPGPGGRGGMRGMPGGNFERGSGGGNTGSADSGG"
     gene            complement(659..1861)
                     /locus_tag="PKY68_07785"
     CDS             complement(659..1861)
                     /locus_tag="PKY68_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOH24968.1"
                     /translation="MKTNMFTSRSGENYLIPFILIASLYLLWGTAHGLLDVLNKQFQG
                     TFNMSKAESGFIQFSTYIGYFIMAIPAGLLLNRVGYKRGIISGLSLFALGAFAFIPAA
                     FFHTPIYFLIALFIIACGLCIIETAAHPYATSMGSESTSAQRINLAAAFNGVGWILGP
                     LIGGTLIFGAEQGDNMAIAKPYIFIGLAVMLVGLVLTFIKLPEIKPEEGEEQAQKPAT
                     SIWSHKNLIFAIIAQFMYVGAQTGIFSFFINYVTELDTSLTNLQASRLLSFGGMGVFL
                     VGRLSSVGLMKKMKPSTLLALFGALAVLCMLLVILSLGTVSVYALYLSFFLMSIMFPT
                     IFALGVRGLGVHTKKASSYIVMSLIGGAIFPVVMGWIGEQNMALGFILPLFAFVYITG
                     FALSQRKF"
     gene            1950..>3264
                     /gene="rlmD"
                     /locus_tag="PKY68_07790"
     CDS             1950..>3264
                     /gene="rlmD"
                     /locus_tag="PKY68_07790"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOH24969.1"
                     /translation="MAKKRKELPLLENVEIEAFAAEGKALARVDNLVVFVPFAAPGDM
                     VDIRLSRKRKQYAEGRIVAFKRYSADRVEPFCSHFGVCGGCQWQHLPYSSQLKYKQQQ
                     VLDVLQRIAKVELPEARPILGSEKDRFYRNKLEYTFSNKRWRTLEEIQSGEEFKNNEA
                     LGFHIPGMFDKVLDIEKCFLQEDFSNRVRLSVKAFCLAHQMDFFDLKLQQGLMRNLIV
                     RTTSTGQKMVIVVFFREETASREALLNHLLNEFPEITSLIYVINNKANDTIFDRETVV
                     YHGSAYIVEEMEGLQFIIGPKSFYQTNSEQAYRLYSLVREFAGLQGNELVYDLYTGTG
                     TIANFLARQCRKVIGIEYIPEAIEDARANAELNGIKNTAFFAADVKDLLNNDFTAEQG
                     RPDVLITDPPRAGMHGDVVKTILSAAPERVVYVSCNPSTQARDLSL"
BASE COUNT          975 a          778 c          750 g          761 t
ORIGIN      
        1 agcaggaatg tctccagcga tgtaatcgat gtggatgacc tgatcctggg tgaccggagc
       61 attaccaata cctataatgc cggtgaaaac ctgtctctga catttaccca tactattatc
      121 gaagccggat tcagaggtcg ttttaattat tcgagaagtc aaaacaacct gaatccggac
      181 aggattcaga ccaccatcaa ctggtcggcc aacgggaatg tgacacttca tctgccttac
      241 gacatcaata ttgataacga tctgagttac agtgcccgcc agggttacgc taattttgac
      301 caaaatgaat tactctggaa tgcccgaatt gacaaagccg tctttaaaaa gagatttacc
      361 ctgcaactga gaataaacga tatcttgaga caacggcaga acattaatca gagtattggc
      421 gacaactccg tctcctacaa taaatcggat atgttgactg cttactacat tgtttctctg
      481 acctaccgca taaggcaatt cggaggcagg gctcccggct ccggccgtgg cggcggcggg
      541 ggattcgctc ccggacccgg aggaaggggc ggcatgcggg gtatgcccgg cggtaatttc
      601 gagagaggct caggcggcgg taataccgga agtgccgact ccggaggata aggtcggatc
      661 aaaatttccg ttgactcaag gcaaaaccgg taatataaac aaaggcaaac agtggaagaa
      721 taaagcccaa agccatattt tgttcgccaa tccagcccat taccacagga aaaatagctc
      781 cgccgatcag gctcatgacg atataggaag aagccttttt ggtatgtact cccagccctc
      841 ttactcccaa ggcgaaaatg gtgggaaaca tgatggacat caaaaagaaa ctcagataga
      901 gagcgtaaac cgaaactgtg cctaaagaaa ggattaccaa aagcatacac agcaccgcta
      961 aagcgccgaa cagagccagc aaggttgagg gtttcatctt tttcatcagc cccacactgc
     1021 taagacgtcc caccaaaaaa acgcccattc caccgaaact gagcaaacgg gaagcttgta
     1081 aattcgtaag actggtatcc aactccgtta cataattgat aaaaaagcta aagatgccgg
     1141 tttgggcgcc cacatacata aattgggcta taatagcaaa tatcagattc ttgtgtgacc
     1201 agatggaggt ggcgggtttc tgagcttgct cctctccttc ctcaggtttg atttcgggca
     1261 gtttgataaa agtcaaaacc agccccacca acatgacagc cagtccgata aaaatatatg
     1321 gcttggcaat agccatatta tcaccctgtt cagctccaaa aattaaagtc cctccaatga
     1381 gcggtcccaa tatccatccc accccgttga aagcagccgc taaattaatg cgctgggccg
     1441 aagtcgattc actgcccatg gatgtggcgt aaggatgcgc cgcggtttct atgatgcaca
     1501 aaccacaggc aatgataaac aaagcaatta aaaaatagat gggtgtatga aagaaagctg
     1561 ccggaataaa ggcaaaggcg cctaaggcaa acaaactcaa accactgata ataccacgct
     1621 tgtaacctac tctgttcaac aaaaggccgg ccggaatagc cataataaaa tagcctatat
     1681 aggtcgaaaa ctggataaaa cccgattcgg ctttgctcat attaaaagtg ccttgaaatt
     1741 gtttattgag tacgtccagc aagccgtggg ccgtacccca aagcaaatac aaactggcaa
     1801 tcagaataaa cggaatcagg taattttctc cgctacggct ggtaaacatg ttggttttca
     1861 tagtcttttt ttaaaaacgc aaatataagc aaaaactgta atcatagctt ttaaattgtt
     1921 atttttgtaa gcgaaaaata tcgctaacat tggcaaaaaa aagaaaagaa ctccccctgt
     1981 tggagaatgt ggaaatcgaa gcttttgctg ccgagggcaa agccctggca agagtggata
     2041 atctggtggt tttcgtgcct tttgcagccc ccggggatat ggtagacatc aggcttagtc
     2101 gcaaacgcaa acaatacgct gaaggccgga ttgtggcctt taagcggtac tcagccgaca
     2161 gggtcgaacc cttttgcagt cattttggcg tttgcggagg ctgtcagtgg caacacctgc
     2221 cctattcttc tcaattgaaa tacaagcaac aacaggtgct ggatgtgctg cagcgaattg
     2281 ccaaagttga attacccgaa gcacggccca ttttgggctc cgaaaaagat cgtttttacc
     2341 gcaacaaact ggaatatact ttttcgaaca aacgctggcg tacccttgaa gaaattcagt
     2401 cgggcgaaga attcaaaaac aacgaggcgc tgggttttca tatacccggt atgttcgaca
     2461 aagtgctcga tattgaaaaa tgctttctgc aagaggattt tagcaaccgc gtaagacttt
     2521 cggtaaaggc attttgtctg gcgcatcaga tggatttttt cgatttaaaa ctccagcaag
     2581 gtctgatgcg caacctgata gtacgcacca cttctaccgg acaaaagatg gtgattgttg
     2641 tttttttccg tgaggagacc gcatcacggg aggccttgct gaaccacctc ctgaacgagt
     2701 tccccgaaat caccagtctc atctatgtga tcaacaacaa agccaacgac accattttcg
     2761 accgcgagac ggtggtttac cacggctcag cctatatagt cgaagaaatg gaggggttgc
     2821 aattcattat agggcctaaa tccttctatc agaccaattc ggaacaggcg taccgcttgt
     2881 attcactggt cagggaattt gccggattac aaggcaatga attggtctac gatttataca
     2941 ccggaaccgg caccattgcc aattttctgg cccggcaatg ccgtaaagtg attggcatag
     3001 aatacatccc cgaagccatc gaagacgcac gggccaatgc cgaactgaac ggcatcaaaa
     3061 acaccgcttt ctttgctgcc gatgtcaagg atttactcaa caatgacttt acggctgaac
     3121 agggccgtcc cgatgtgctt atcaccgatc ctccccgagc cggtatgcac ggagatgtag
     3181 ttaaaaccat tttgtctgcg gctcccgaac gtgtggtgta cgtgagctgc aatccctcca
     3241 cccaggcaag agacctgagt ctga
//
LOCUS       DAOSGL010000262         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_27669_length_3254_cov_5.122538, whole genome shotgun sequence.
ACCESSION   DAOSGL010000262 DAOSGL010000000
VERSION     DAOSGL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27669_length_3254_cov_5.122538"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2690
                     /locus_tag="PKY68_07795"
     CDS             <1..2690
                     /locus_tag="PKY68_07795"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOH24970.1"
                     /translation="DGSEPATSSTAINAGTWREWPIDPTQPILRGQTPAYVLRACLVK
                     EGYKPSVSITRTYIFTDQVKTQTHPGWEWPVGRHNGQRLDYEMDTAVVNHKNYSPYID
                     AALRQIPSISIVTDMKHLFDAGSGIYVNAEKSGRAWERPCSIELIDTCETKGFQINAG
                     IRIRGAASAKQKESVKRAFRFFFRKEYGASKLKYPLFDNEGVEEFDRMDLRCEQNYSW
                     SKDGSKYNSMIADIFSRDLQGRMKQPYKRGRYYHLYLNGMYWGLYQTDERAEANNAEH
                     YFGGHKDDYDVIKVYSEFWPYTNEATDGNLEAWTALWNKCTEGFASKEAYLALLGKKP
                     DGSMDPEGRVWVDIDNLIDYMLVIFYTGNFDAPVSAFYNDDMPNNYYAVYNRNDHSRG
                     FRFFAHDSEHSMFVDNVFVGGLNCNRVNIGTNGSMQINRMLEFNPQWLHYKLCSNEEY
                     RFRFMDRAYKYLSVGGLLRPDSAAALYRSRAAQIDLAVIAESARWGDAVSHTTLGKKD
                     WETLLQSMYNEFFPKRTNILIQQLKAEGLYTTIAAPGISINNYRFYGSDYSFRGEVPV
                     SISNLSGPSSSLYYTLDGSDPRTFGGGISSKAINVRGSVSFSINQSTMLSCRIYSGTN
                     WGPLKQVFFSNADEDYQALKITEIHYHPKDTFVNSVLTKGQDFEFIEFKNTGDKAIDL
                     SGLKIDSGIYHRFPEQQLLYPGEFYVAASKPNNFYLRYGRYPSGSFSRNLSNSGEKIV
                     LLDPANQPLISFDYYDKSPWPTEPDGLGYSLAAAQAMPEGDPGDYSYWITSSHVGGSP
                     FADDPASASAIKSPGTFLAARLDVYPNPSKGTFFVNTGQNESYRLRIYDSKANLIFDR
                     QYEGIAAIDFGTENLSRGLYILRAELPGEVLSTKLIYLP"
     gene            2929..>3254
                     /locus_tag="PKY68_07800"
     CDS             2929..>3254
                     /locus_tag="PKY68_07800"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOH24971.1"
                     /translation="MKRILFVFSFFLVSCINLSATKILILQPDSVSGKDAFVHSFHPD
                     ENYGKHHLLQALNWTISGLDAKLRFFIDFDLSSIPEKAIIVSAKLSLFESHENAASEG
                     HSHLSGP"
BASE COUNT          898 a          829 c          714 g          813 t
ORIGIN      
        1 tcgacggcag cgaacccgcc acttcttcta ccgccatcaa tgccggcacg tggagagaat
       61 ggcccatcga ccccacacaa cccattttga gagggcagac gccggcttat gttttgcgcg
      121 cctgcctcgt gaaagagggt tataagcctt cggtttccat taccagaacc tatattttta
      181 ccgatcaggt aaaaacccag actcaccccg gctgggaatg gcctgtcggc cggcacaacg
      241 gacaaaggct cgactacgaa atggatacgg ccgtggtaaa ccacaaaaat tattcgccct
      301 acattgacgc agcccttagg caaatcccca gcatcagcat agtaaccgac atgaagcatc
      361 tgttcgatgc cggcagcggc atctatgtga acgccgaaaa aagcggccgg gcctgggaac
      421 gtccctgttc catagaattg atcgacacct gcgaaaccaa aggctttcaa ataaacgcgg
      481 gtattcgcat caggggagcc gccagtgcta aacaaaaaga gagcgtcaaa agggcttttc
      541 gtttcttctt cagaaaagaa tacggagcct ccaagctgaa ataccccctt ttcgataacg
      601 agggagtaga agaattcgac cgcatggact tgcgctgcga acaaaattac tcctggagca
      661 aagacggcag taaatacaat tccatgatag ccgatatttt cagtcgcgac ctgcaaggcc
      721 gcatgaaaca accctacaaa agaggacgct attaccatct ctacctcaac ggcatgtact
      781 gggggctcta ccagaccgac gaacgggccg aagccaacaa tgccgagcat tatttcggcg
      841 gacataaaga cgactacgac gtcatcaaag tctattccga attctggccc tatacgaacg
      901 aagccaccga cggcaacctg gaagcctgga cagctctgtg gaataaatgt accgaaggct
      961 ttgcctccaa ggaagcctat ctggccttgt tgggcaaaaa acccgacgga agcatggatc
     1021 cggagggcag ggtttgggtg gatatcgata atctgatcga ctatatgctg gttattttct
     1081 acaccggcaa tttcgatgcc ccggtttcgg ccttttacaa cgacgacatg cccaacaatt
     1141 attacgccgt atataaccgc aacgaccact cgaggggatt ccgttttttt gcgcacgatt
     1201 cggaacacag tatgtttgtt gataatgtct tcgtaggtgg attgaattgc aaccgtgtga
     1261 acataggaac aaacggcagt atgcaaataa accgtatgct cgagtttaat cctcagtggt
     1321 tacactacaa actctgctcc aacgaagaat accgctttcg ttttatggac cgtgcgtata
     1381 agtatttatc cgttggcggt ctgttaaggc ccgattctgc cgcagccctc tatcgctcaa
     1441 gagccgccca aatagacctg gctgttattg ccgagtcggc ccgctgggga gacgccgtga
     1501 gtcatacaac cctgggtaaa aaagactggg aaaccttgct tcagtctatg tataacgaat
     1561 ttttcccaaa acgcaccaac atcctgatac agcagctcaa agcagaaggc ttgtatacca
     1621 ccatagcggc tccgggcatc agcatcaaca attatcgctt ttacggttcg gattacagtt
     1681 ttcggggcga agtgccggtt agcatcagta atctttccgg tccaagcagc agtctttact
     1741 acacgctcga cggcagcgac cccagaactt tcggaggcgg aatttcgtcc aaggccatca
     1801 atgtcagagg cagtgtttcg ttcagcataa accaaagcac tatgctgtcg tgcagaattt
     1861 actcgggaac caactggggc cccctgaaac aagtcttttt cagtaatgca gacgaagatt
     1921 accaggcctt aaagattacc gaaatacatt accaccccaa agacactttt gtaaattcgg
     1981 tattgaccaa gggacaagat tttgagttta tcgagttcaa aaacaccggc gacaaagcca
     2041 tagatctgtc cggactgaaa attgactcgg gcatttatca ccgctttccc gaacaacaat
     2101 tgttgtatcc gggcgaattt tatgtagccg cctccaagcc caacaacttc tacttgcgct
     2161 atgggcgcta tccctccggc agtttttcga gaaacctttc caattcgggc gaaaaaatcg
     2221 ttttgctgga tcccgctaat caacccctga tcagtttcga ttactacgac aaaagcccct
     2281 ggcccacgga gcccgacgga ttgggatata gcctggcagc cgcacaggca atgcccgaag
     2341 gagatcccgg cgattattcc tactggataa cctccagtca cgtgggcgga tcgccattcg
     2401 ccgacgaccc cgcatctgcc tctgccatca aaagccccgg cacattcctc gccgcccgcc
     2461 tcgatgtcta tcccaacccc agcaaaggca ccttcttcgt taataccgga caaaacgaaa
     2521 gctatcgcct gcgaatatac gatagcaaag ccaacctaat cttcgacaga cagtacgaag
     2581 gcatcgccgc aattgatttt ggtaccgaga acctcagccg cggtctttac atcctccgcg
     2641 ccgaattgcc cggtgaggtg ttgagcacta agttgattta tttgccttga tcggaatgaa
     2701 aaatgaaagt attaaacaag caaaaacatg ccattctttt tcatcaatac agggtgtatc
     2761 cgagttgttt tttaaagaaa gactatatta ttttaggtgt tttgttgttt attcaagaat
     2821 ttttgtgaca tttgtcaatg aaaagggtaa aacatttcta cactgaactc gatcaattaa
     2881 ttgagcgaat ttaaatagcg tattgacctt agtataaacc tgatagccat gaagcggatt
     2941 ttatttgtct tttctttttt tctcgtttct tgtatcaatt tgagtgccac aaaaatatta
     3001 attcttcaac ctgattctgt atcgggtaag gatgcttttg ttcattcatt ccatcccgat
     3061 gaaaactacg ggaaacacca tttattacaa gcacttaact ggacaatttc tggtttggat
     3121 gctaaactca gatttttcat tgactttgat ttgtcttcca ttcccgagaa ggcaataatt
     3181 gtatctgcta aactttctct ttttgaatct catgaaaatg ctgcatctga agggcatagt
     3241 catttatcag gtcc
//
LOCUS       DAOSGL010000263         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28047_length_3224_cov_4.822973, whole genome shotgun sequence.
ACCESSION   DAOSGL010000263 DAOSGL010000000
VERSION     DAOSGL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28047_length_3224_cov_4.822973"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKY68_07805"
     CDS             complement(<1..677)
                     /locus_tag="PKY68_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOH24972.1"
                     /translation="MAVFIINSSIGRKLIMSITGAALVLFLTFHSIMNVFVIISPKVY
                     DAICYFLGANWYALIATLVLAFLTVVHVLYAFWLTLQNKIARGRDAYSVNKRPKGVEW
                     ASENMLVLGLIILGFLALHLYNFWYKMQLTELLGKINGEEISVAGAALVQGLFQSPVY
                     CLLYMVWLTALWFHLTHGVWSALQSIGWSNDIWLPRIKVISNVVSTIVVGLFMLVPIY
                     YLIVNFIL"
     gene            841..1737
                     /locus_tag="PKY68_07810"
     CDS             841..1737
                     /locus_tag="PKY68_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOH24973.1"
                     /translation="MNTNQSKRLKETICQLSQYESFKELRHERAYSDTLPSTLKLEEI
                     IRICRGILFPGYFGNASVNKQNLAYHMGLNTEKLLVLLSEQIAAGLSFFPRDKYQNAT
                     ELKEKADTVAEEFIFNLPELRKILYTDIMATYNGDPAAESIDEVIYCYPGIRAISSYR
                     IAHLLLKLDVPIIPRIITEMAHSETGIDIHPAASIGHSFSIDHGTGVVIGATAILGNH
                     VKLYQGVTLGAKSFSLDDKGNPVKGVPRHPILEDNVIVYSNATILGRITIGENAVVGG
                     NIWVTEDVPPGARVVQSKRISE"
     gene            1744..2898
                     /locus_tag="PKY68_07815"
     CDS             1744..2898
                     /locus_tag="PKY68_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOH24974.1"
                     /translation="MREDSFEMLAKTLQGLEGVLAEEIVQLGGNNVKPAKRSVFFEGD
                     KSLMYKANLRLRTALRILKPIAVFDAAHPDQIYEAVKAVDWKNFMDSDQTFMVETVVY
                     SDTFRHSNFVSYRVKDAIADHFREKTGKRPSVQLVNPDLYIHLHIARKHCTLLLDSSG
                     ESLHKRGWRVKQTEASLNEVLAAGMLLLAGWKGQCPLTDPMCGSGTLLIEAALIALDI
                     PPGIYRRHFAFEKWKDFDRELFRTLYEEENRKEFRFRIQGSDISARAIKIAAENIKNA
                     GLSKYIDLKVQGVQEITQAPPNGLLVCNPPYGERLDNNKLNELYQSLGERLKHVFAGN
                     TAWILSSRKDLLNCLSLRHSARHFLMNGALNCEYRRYDIFSGKKKDQFTQ"
     gene            2913..>3224
                     /locus_tag="PKY68_07820"
     CDS             2913..>3224
                     /locus_tag="PKY68_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOH24975.1"
                     /translation="MAKKNNFYVVWQGRKPGIYSSWEACKSEVEAFESARYMGFETQE
                     QAKQAFKEGWKKHYALKSKGSNPKPGTRPTGEALTVDAACSGNPGRMEYRGVLLQNGK
                     EV"
BASE COUNT          966 a          772 c          739 g          747 t
ORIGIN      
        1 agtataaaat ttacaatcaa ataataaatg ggaaccagca taaagaggcc caccactatg
       61 gtcgatacaa cattggaaat taccttgata cgcggcaacc agatgtcgtt gctccagccg
      121 atgctttgca gggcactcca gaccccatga gtcaggtgaa accacaaggc ggtcagccat
      181 accatgtaaa gcaggcagta aacaggactt tgaaacaagc cctgaactaa ggccgctccc
      241 gcaacactga tttcttctcc gtttattttg cccagcagct cggtcagttg cattttatac
      301 caaaagttgt acaaatgcaa ggccagaaag cccaggatta tcaaacccaa taccaacatg
      361 ttttccgaag cccactcaac acccttgggc cgcttgttta ccgagtaggc atccctgccg
      421 cgtgctattt tgttttgcag ggtaagccag aaggcataca aaacgtgcac cacagtaagg
      481 aaagccagga ccagagtagc aatcaacgca taccagttgg ctcccaaaaa ataacagatg
      541 gcatcataca ccttgggtga aatgatgaca aaaacattca taatactgtg gaaggtgaga
      601 aaaagcacta aggcagctcc tgtaatactc ataatgagct ttcttccaat tgatgaatta
      661 ataataaaaa cagccatatc cgatttaaat tagcaagttt taatctcctc gagtgagaag
      721 cggcaaaagt acgtcgtttt tgggaaaccg gcaaacaatt aaagaaatat ttccggaata
      781 ccaaaagctc tcatcaagta aaaaaattac gtatttttgc cgaggaaata tactacaatt
      841 atgaatacta accaatccaa aaggttaaaa gaaaccattt gccaattgtc tcaatatgaa
      901 tcgttcaaag agctgaggca tgagcgggct tattccgaca cgctaccctc gacccttaag
      961 ctggaggaaa ttatccggat ttgccgcggc attctttttc ccggttattt tggaaacgcc
     1021 tctgtcaaca aacaaaacct ggcgtatcac atggggctta acaccgaaaa attactggtc
     1081 ctgctgagcg aacaaatcgc tgccggattg agttttttcc ccagggacaa ataccagaac
     1141 gccactgaac ttaaagaaaa ggccgatact gttgccgagg agtttatttt taatttaccc
     1201 gagttgcgca aaattctcta taccgacatt atggccacct acaacgggga tcctgcagcc
     1261 gaaagcatcg atgaagtcat ttattgctat ccgggcatca gggcaatcag cagttaccgg
     1321 atcgcccatc ttttgctaaa gctcgacgtg cccattattc ccagaataat taccgaaatg
     1381 gcgcattccg aaaccggcat cgatattcac ccggcagcga gtatagggca ttcgttttcg
     1441 atcgaccacg gcacaggagt ggttataggg gctacagcca tcctggggaa ccatgtgaaa
     1501 ctttatcagg gtgttacgct gggtgccaaa agcttttcgc ttgacgataa gggtaatccc
     1561 gtcaaaggag ttccccgcca ccccatactc gaagataatg ttattgtcta ctccaacgcc
     1621 accatactgg gaagaattac tataggcgaa aacgctgtag tggggggaaa tatctgggtc
     1681 accgaagatg ttcctccggg agcacgggtt gttcaatcca aacgcatctc tgaataagca
     1741 accatgcggg aagatagttt tgaaatgctg gccaaaactc tccaaggcct ggaaggagtg
     1801 ctggccgaag aaattgttca gttgggtgga aacaatgtaa aacccgctaa acgctccgtt
     1861 ttctttgagg gagacaaaag ccttatgtac aaggctaatc ttcgtctgag aaccgctctt
     1921 cgcatactta aacccatagc cgttttcgat gccgcccatc ccgaccagat ttacgaagcg
     1981 gtcaaagccg ttgattggaa gaattttatg gattccgatc agactttcat ggtagaaact
     2041 gtagtttact ccgacacctt caggcattcc aattttgttt cttacagggt aaaagacgct
     2101 attgctgacc acttcagaga gaagaccggt aaacgcccct cggtgcaact ggtcaatccc
     2161 gatttgtaca tccacctgca catcgcccgg aagcactgca ctctcttact cgacagttcc
     2221 ggagaatcgt tgcacaaaag aggctggcgg gtaaagcaaa ccgaggcttc actcaacgaa
     2281 gtactggccg ccggtatgtt gctgctggcg ggctggaaag ggcaatgccc tctgacagac
     2341 cccatgtgcg ggtcgggtac cctactaatc gaggcagcct tgattgccct ggatatccct
     2401 ccggggatct atcgccggca ttttgccttc gaaaaatgga aagattttga ccgggaactt
     2461 ttccgtacac tctacgaaga agaaaaccgg aaagaatttc gttttcgcat tcagggcagc
     2521 gatatttcgg cacgggcaat aaaaattgcg gccgaaaaca taaaaaatgc tggtttgagc
     2581 aaatacattg acctgaaagt tcaaggcgta caagaaataa cacaagcgcc gcccaacggt
     2641 ctgctggtgt gtaatccgcc ttacggggaa agactcgaca acaacaaatt aaatgaatta
     2701 taccaatccc tgggcgaaag gctgaaacat gtttttgccg ggaacacggc ctggatactg
     2761 agttcccgca aagatctgct caactgttta agcctcaggc attcggcacg tcattttttg
     2821 atgaatggag cattaaactg cgaatatcgc cgttacgata ttttcagcgg gaagaaaaaa
     2881 gatcagttta cccaatagaa tagaacctag caatggctaa aaaaaacaac ttttacgtgg
     2941 tttggcaggg tcgcaaaccg ggcatctact caagttggga agcctgcaaa tctgaggtag
     3001 aggcttttga gtcggctcgt tatatgggtt ttgaaactca ggaacaagcc aaacaagcct
     3061 ttaaggaggg ttggaaaaaa cactatgccc ttaaaagcaa gggcagcaac ccgaagcccg
     3121 gaacccgccc caccggagaa gcgcttaccg tcgatgccgc ctgcagcgga aatcccggta
     3181 gaatggaata ccggggggtc ttgcttcaaa acggcaagga ggtg
//
LOCUS       DAOSGL010000264         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28326_length_3203_cov_3.736023, whole genome shotgun sequence.
ACCESSION   DAOSGL010000264 DAOSGL010000000
VERSION     DAOSGL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28326_length_3203_cov_3.736023"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1597
                     /gene="cysS"
                     /locus_tag="PKY68_07825"
     CDS             104..1597
                     /gene="cysS"
                     /locus_tag="PKY68_07825"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOH24976.1"
                     /translation="MMQQLSIYNTLSRQKEVFKPLHQGRVGLYVCGPTVYGDPHLGHA
                     RPAITFDILFRYLSRLGYKVRYVRNITDVGHLENDADEGEDKIAKKARLEELEPMEVV
                     QYYLNRYHKAMEALRVLPPSIEPHASGHIIEQIRMVQKILDNGYAYVSEGSVYFDVEK
                     YNKSHHYGKLSGRNIEDMLSATRSLEGQDEKKNAMDFALWKKASPEHIMRWPSPWSDG
                     FPGWHIECTAMGTRYLGEEFDIHGGGMDLLFPHHECEIAQSVAALNKESVRYWMHNNM
                     ITINGQKMGKSLGNFITLEEFFNGSHPMLTQAYAPMTIRFFILQAHYRSTVDFSNEAL
                     QASEKGCSRLMDIYSRLQELEPAETSTVNLSDLRQKCFDAMNDDLNTPVVISHLFEAG
                     KAVNSIFDGNAAISAGDLKELREVFALFVSEILGLDPSTASQDNAKAEAERAAYKAVV
                     DLLLELRLEAKANKDWAQADKIRKQLAELGFKVMDTKEGFDWKWSAP"
     gene            complement(1634..2548)
                     /locus_tag="PKY68_07830"
     CDS             complement(1634..2548)
                     /locus_tag="PKY68_07830"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOH24977.1"
                     /translation="MKIPSILYLLVAGLIMAQNLSGKEPCSLQNTSFNAGEKIRYQVY
                     YNIGFIWIAAGVCDFEAKMTTWTAKTTTPKTGPVTGKNSLVTGKNSPAPLNSHPALHL
                     KATGVTHRAYDAFFTVRDTLESYINPFNLIPYASIKYTHEGKWHGQDNFLFQPSDTSE
                     GWNIRTQLKRKGVWRDPTFSHTKDCGFDIISSVYRMRSIPDSLLFSKQAIRIPVRLDD
                     GQYDVTLSYKGKALIKLRRGNTYYAHYFELSLVEGTVFKRGDVLKLWLGNDPNRLPLQ
                     VESPIRVGSVKAVFERAENLRHVQEKLN"
     gene            complement(2558..>3203)
                     /locus_tag="PKY68_07835"
     CDS             complement(2558..>3203)
                     /locus_tag="PKY68_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24978.1"
                     /translation="KPTLEIYSTLHFTWETPIRNIHGDPFRLYRKKDTLWIETKDFTL
                     EPHPEKLRTYLLDALWDFEAQYKVEIDSGCVEGWYGKTNAKFSQSFNIRAEKDYSRLT
                     VNISGFSNPAFAELLDKSDNVVRKARIEEGVADFPFIKPGDYYLRAVDDANDNFLWDT
                     GHYASKRQPEKVFYNNKLFNLKANWEIKESWDIRDLPLIEQKPKDLKPAEPKNR"
BASE COUNT          796 a          746 c          800 g          861 t
ORIGIN      
        1 gccaggacta cagaatgatt gttaccactt gttccggatt gtggagatat tgctccgaag
       61 gcccggctat tcggtttgtt tcggagaagc cctacaaatt tgtattatgc aacaactgag
      121 tatttacaat acattaagcc ggcaaaaaga agtttttaaa cccctgcacc aaggccgtgt
      181 ggggctttat gtctgtggcc ccacggttta cggcgatccg catctgggtc atgcccgtcc
      241 ggccataacc ttcgatatac tgtttcgtta cttaagccgg ttgggctata aagtgcgcta
      301 cgtccgcaac attaccgatg taggccattt ggaaaacgat gcagacgaag gagaggataa
      361 aattgctaaa aaggcccggc tggaagagct cgaacccatg gaggtggtcc agtattattt
      421 aaaccgctac cacaaagcaa tggaggcact ccgggttttg cctcccagca ttgagcctca
      481 tgcatcgggg catattattg agcagatacg gatggtgcaa aaaatcctcg acaacggtta
      541 tgcgtacgtg agcgaaggct ctgtctattt tgatgttgaa aaatacaaca agagccatca
      601 ttacggcaag ttgtcgggac gcaacatcga agatatgctc agtgccaccc gcagtctcga
      661 aggccaggac gaaaagaaga acgctatgga cttcgctttg tggaagaagg cctcgcccga
      721 acatataatg cggtggcctt cgccttggag cgatggtttc cccggttggc atatagaatg
      781 taccgctatg ggcacccgct atctgggaga agaattcgat attcacggcg gtggaatgga
      841 tttgcttttt cctcaccacg aatgcgaaat agcccaatcg gtggcagccc tcaacaaaga
      901 aagtgtccgc tattggatgc acaacaatat gattaccatc aatgggcaaa aaatgggcaa
      961 gtcgcttgga aatttcatta cactggaaga atttttcaac ggcagccatc ccatgctgac
     1021 gcaggcctac gctccaatga ccattcgctt ttttatttta caggctcatt atcgcagcac
     1081 agttgacttt agcaacgaag ccctgcaggc ctccgaaaaa ggttgcagcc gcctgatgga
     1141 tatttacagc cgcctgcagg aacttgagcc ggcagaaacc tcgaccgtaa acctcagtga
     1201 tttgagacag aaatgcttcg acgccatgaa cgatgatctg aatacacccg tggttatctc
     1261 gcatctcttc gaagcaggca aagccgttaa cagcatcttc gacggtaatg ccgctatttc
     1321 agccggtgat ctgaaagaac ttcgcgaagt ttttgcgctc tttgtcagcg aaatactagg
     1381 cctggatccc tcaacagctt cgcaggacaa tgcaaaagca gaagccgagc gggcagccta
     1441 caaggcagtt gtagatcttt tgctggagct acgtctcgaa gccaaggcca acaaggactg
     1501 ggcacaagcc gataagatac gcaaacaatt ggcggagctg ggctttaaag tgatggacac
     1561 aaaagagggc tttgattgga aatggtctgc tccctgatta gttgaaattc ccgattggcc
     1621 ggaatccccg attttaattt agtttttcct gcacatgccg taaattttcg gcacgttcga
     1681 agacggcttt taccgaaccg acgcgaatag gcgattctac ttgcagcggc aggcggttgg
     1741 gatcgttgcc cagccaaagt ttcagcacat ctcctctttt aaatacagta ccctcgacaa
     1801 gactcagttc gaaatagtgg gcgtaatagg tgttgccccg gcgcagtttt atgagcgctt
     1861 tgcctttgta gctcagcgta acatcgtact ggccgtcgtc aagcctcacc ggtatacgaa
     1921 tagcctgttt cgagaaaagc aaggaatcgg gtatgctgcg catgcggtaa acgctgctta
     1981 ttatatcgaa accgcaatct ttagtgtggc tgaacgtggg atccctccaa actcccttgc
     2041 gttttaattg tgtgcgaata ttccatccct cactggtatc ggatggttgg aacaggaaat
     2101 tgtcctggcc gtgccactta ccttcgtgcg tatattttat acttgcgtag ggaataagat
     2161 tgaagggatt gatgtatgat tccagggtgt ctctgaccgt aaaaaaagca tcgtaagctc
     2221 tgtgggtaac gcccgtagct ttgagatgca gagcaggatg gctgttcaac ggggcagggc
     2281 tgttttttcc ggtgacgagg ctgttcttcc cggtgacggg accggttttt ggggtcgtgg
     2341 tttttgccgt ccaggtggtc atcttggctt caaaatcaca aactcccgcg gcgatccata
     2401 taaagccgat gttgtaatag acctggtagc ggattttttc tccggcatta aagctggtgt
     2461 tttgcaagga acatggttcc ttgccggata gattctgagc cataataagc cctgcaacca
     2521 ataaataaag gatggaagga atcttcatac caccgtttta tctgtttttg ggttcggccg
     2581 gtttcaggtc tttgggtttt tgctcaatca ggggcaggtc acggatatcc cagctttcct
     2641 tgatttccca gttggccttg agattgaata gtttgttgtt gtaaaacacc ttctcgggct
     2701 ggcgtttcga ggcgtaatgg cctgtatccc aaaggaaatt gtcgtttgca tcgtccacag
     2761 ccctgagata gtaatcgccc ggtttaataa acgggaaatc agccactcct tcttctattc
     2821 ttgctttgcg taccacattg tcgcttttgt cgagcaactc ggcaaaggca ggattgctaa
     2881 aaccgcttat gtttaccgta aggcgcgaat agtctttttc ggcccgtatg ttgaaacttt
     2941 ggctgaattt tgcgttcgtt ttaccgtacc atccttccac gcaacccgaa tcgatttcta
     3001 ctttgtattg ggcttcgaaa tcccagagag catccaacag gtaagtcctt agtttttcgg
     3061 gatgaggctc caaagtgaaa tccttggttt ctatccagag cgtgtctttt ttgcgataca
     3121 atctgaaggg atccccatga atgtttctta tcggcgtttc ccaggtaaaa tgcagtgttg
     3181 agtatatttc cagagttggt tta
//
LOCUS       DAOSGL010000265         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28352_length_3201_cov_3.295613, whole genome shotgun sequence.
ACCESSION   DAOSGL010000265 DAOSGL010000000
VERSION     DAOSGL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28352_length_3201_cov_3.295613"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKY68_07840"
     CDS             <1..717
                     /locus_tag="PKY68_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOH24979.1"
                     /translation="EITTLFPSKYIHIGGDEVHFGNQQWNYLPEIQELMKAEGLENLV
                     AVEHYFLNRMSDSIRTLNKTVMGWDEVVTAGLPASNTVVMWWRQERPEQLEKAISGGY
                     EIVLCPRLPLYLDFVQHPSHEYGRKWSKGEHAPMEKVYHFPGTSYTSGISIATPLIKG
                     IQGNAWTEVIHTPERLQFMVYPRLSALAEAAWTQDRSKNYENFNLRMEKMMEFYKKSG
                     IVFFDYKNPDLSPEVAGPKK"
     gene            737..1855
                     /locus_tag="PKY68_07845"
     CDS             737..1855
                     /locus_tag="PKY68_07845"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOH24980.1"
                     /translation="MESNQTKDAITSLSKEQGRILSIDFFRGLVMFILVSGIATLFHQ
                     MTEQGQGGAFIAWIEKHSHHGDWFEVYFWDLIQPFFMFIVGVAMPFSLSKRLARGDSW
                     KQSFIHALKRSFWLLLLGFMLGAKDNAYYLTNILPQLGLVYVFAFLLLQKNVKWQLLV
                     SFALILVSDLLYQFWPVEGYNMPYTPGHNFGVWFDSVTVGHLHPGNWVTFNAVPTSAH
                     ILWGVCTGKLLMTDWSQKKKLLIMLSTGIAGIIGGYLLGLYIPMIERISTSSFVIFSG
                     GWCLLAMGLSYLFVDIFRFRKIATFFAIVGMNPIFIYIFTSLGGRNLLTRMARPFTYR
                     LFSWGGDLVINMVTIVVVAFMVWGICYYMYKRKIFIKL"
     gene            1870..3048
                     /locus_tag="PKY68_07850"
     CDS             1870..3048
                     /locus_tag="PKY68_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOH24981.1"
                     /translation="MKRIHFVWVLFLLYVLSVNISALSAKEPERVILFMIDGLHWQAP
                     EKLNMPVLNSLIKEGTYVRKSYMIIPHHPTVGDYSLSNSCSFPNPMMHEGTIFLSPKN
                     KMIQEMISPKQQTAFVVNTTAYRSVGRGFSTCIMDNSLTDDQAVQQAIHLLESQQIRF
                     MRVHLQSPGGIGTNIAMFSEGKPYARDIFGEGSPYINAIENADRLLGELIDYLKKRGE
                     WESTVLIVTSDHGQSKVGWHPMLDEDSWVTPLVFVGTGIAKGRELPYFEHTDLAPTIA
                     RLLGVKAPNTGGGAGKVVEEIMEKRDAASYNPNQFIKIINQQIREYNLLYAQLVLVAE
                     NNNYVANIISSLSNENLTPEPFYDQDRITEWHRAGSTEHLIEANEAVLYKMKETLLIK
                     "
BASE COUNT          865 a          683 c          798 g          855 t
ORIGIN      
        1 gagataacca ctcttttccc ctcaaaatat atccatatag gtggagatga ggtacatttc
       61 ggcaatcaac aatggaatta cctgcctgaa atacaagaac tcatgaaagc agagggtctc
      121 gaaaacttgg tagctgtgga acattacttc ctaaacagga tgtcggactc cattcgcact
      181 ttaaacaaaa ccgttatggg ttgggatgaa gtggttactg ccggcttgcc tgcatctaac
      241 acggtggtta tgtggtggcg tcaggaaagg cctgaacaac ttgaaaaagc gataagcgga
      301 ggttacgaaa tagtgctgtg tccgcgacta ccgctttatt tggatttcgt ccagcatcca
      361 tcgcatgaat acggtcggaa atggtcgaaa ggagagcacg cacccatgga aaaagtgtat
      421 catttccccg gtacgagtta cacttcgggc atatctatag ccacaccgtt gattaaagga
      481 atacaaggaa atgcgtggac ggaagttata catactcctg aacggttgca gtttatggta
      541 tatccccgac tgtcggcctt ggcggaagct gcatggacac aagatcggtc aaaaaattac
      601 gagaacttca atttgcggat ggagaagatg atggaatttt acaaaaaatc aggtatcgtt
      661 ttcttcgatt ataaaaatcc cgatttaagt ccggaagttg caggaccgaa aaagtgataa
      721 ataagaatta aaagagatgg aatcaaatca aacaaaagat gcaattacca gcttgagcaa
      781 ggaacaggga agaatccttt ccatcgactt cttccgggga ttggtgatgt ttatcctggt
      841 atcggggata gcgacgctgt ttcaccaaat gacggaacag gggcagggag gcgcgtttat
      901 cgcctggatt gagaagcact cccaccatgg cgattggttc gaagtctatt tctgggacct
      961 gatacaacct ttttttatgt ttatcgtagg tgttgccatg cctttttcac tttcaaaaag
     1021 gcttgcccgg ggcgatagtt ggaagcaatc gtttatccat gccttgaagc gttccttctg
     1081 gttgcttctg ctcggtttta tgctgggagc aaaagacaac gcctattatt tgaccaatat
     1141 ccttccccaa ctggggctgg tgtatgtgtt tgcatttctg ttgctgcaga agaatgtaaa
     1201 atggcaactg ctggtttctt tcgcgctgat actggtctcc gatctgcttt atcagttttg
     1261 gccggtagaa ggatataaca tgccctacac tcccggtcac aattttgggg tatggttcga
     1321 ttcggtcacg gtaggtcatc tgcatcccgg caattgggtt acattcaacg cggtgcctac
     1381 ctccgcccat atcctttggg gtgtatgtac cggtaaattg ttaatgacgg attggtccca
     1441 aaagaaaaaa ctattgatta tgctttcaac cggaatcgcc gggatcatag gcgggtacct
     1501 gcttgggtta tacataccca tgatagagcg tatcagtacc agctcttttg tcatttttag
     1561 cggtggttgg tgcttgttgg caatgggatt atcctatctc ttcgtggata tattccgctt
     1621 caggaagatt gccacgttct ttgccatcgt gggaatgaat cctattttta tttacatttt
     1681 tacaagcctc ggtggtagga atctacttac ccggatggct cgccctttca cgtaccgtct
     1741 tttcagctgg ggaggagatt tggtgatcaa catggtgacg attgtggtgg tagcgtttat
     1801 ggtctggggt atctgctatt atatgtataa acggaagatt tttattaaat tgtaaaataa
     1861 gagaataaca tgaaaagaat tcattttgta tgggtattgt ttctgctgta tgttttgtcg
     1921 gtgaatatat cggctttatc ggcaaaggaa cccgaaaggg tgatcttgtt catgatagac
     1981 ggattgcact ggcaggcgcc cgaaaagttg aatatgccgg tactcaattc cctgataaag
     2041 gagggtactt acgtgcgaaa atcgtacatg atcattccgc atcaccctac ggtaggagat
     2101 tacagcctat cgaacagttg ctcctttcct aacccaatga tgcatgaagg taccatcttt
     2161 ctgagtccaa aaaataaaat gatccaggaa atgatctccc caaagcaaca gacggcattc
     2221 gtggtgaaca ccaccgccta tcgttccgtg ggaaggggct tctccacctg catcatggat
     2281 aactcgctga ccgatgatca ggcggtgcaa caggctatcc atttgctgga aagtcagcag
     2341 atccgtttta tgcgggtcca cttgcaaagc cccggcggta tcggtacgaa tatagccatg
     2401 ttcagtgaag ggaagcctta cgcgagagat attttcggtg aaggctcccc ttatatcaat
     2461 gccattgaga atgccgacag gctgctggga gagttgatcg attacctgaa aaagagggga
     2521 gaatgggaga gtaccgtttt gattgtgacc tccgaccacg gacagagcaa ggtgggttgg
     2581 catcccatgc ttgacgagga tagctgggtg acgccgttgg tctttgtcgg aaccgggatc
     2641 gccaaaggac gtgaacttcc ttactttgag cataccgatc tggcgccgac gatcgcccga
     2701 ctgttgggcg tgaaagcccc gaacactggc ggcggggcgg gaaaagtcgt ggaagagatc
     2761 atggaaaaga gggacgcggc atcctacaac cccaaccaat ttatcaaaat aatcaaccaa
     2821 caaattcggg aatacaatct cttatatgcc caattggtgc tggttgccga aaataacaac
     2881 tacgtggcca acatcatctc ctccctttcc aatgaaaacc tcactcccga gccgttttac
     2941 gatcaggaca ggataacgga gtggcacagg gcgggttcaa cggaacacct gatagaggcc
     3001 aacgaggccg ttctgtataa aatgaaagaa acattgctta tcaaataaat ctatggcaca
     3061 aaatctattg attaaaaatc ggtcacggtt cggtatcgct tttcttggga tgatttccat
     3121 ttgctgcctt tacaccacga ccgtacatgc gcaggatgtg gaaaagatgg cgaaacaaat
     3181 agctttcgag caagttttcg g
//
LOCUS       DAOSGL010000266         3200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28358_length_3200_cov_4.744356, whole genome shotgun sequence.
ACCESSION   DAOSGL010000266 DAOSGL010000000
VERSION     DAOSGL010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28358_length_3200_cov_4.744356"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PKY68_07855"
     CDS             complement(<1..471)
                     /locus_tag="PKY68_07855"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH24982.1"
                     /translation="MDLRAKILAYFPYTPTSDQHKALDSCLNFFMFSGEMEVLLLKGY
                     AGTGKTALISAVVRFLTENRQPCVLMAPTGRAAKVFASYSGHAATTIHKKIYRQKEVD
                     GTYGRFDLNDNLHENTFFFVDEASMISNENFEGSFFGSGRLLDDLLRYVYSGKRC"
     gene            591..1265
                     /locus_tag="PKY68_07860"
     CDS             591..1265
                     /locus_tag="PKY68_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24983.1"
                     /translation="MRLFFRILLIIAVGLSVYLCVDSILRPIHFNREKDIRFNAVIGR
                     LIDIRKAQVEFRNYHGLYANDFDTLIGFVKHGYIPFVLKEGVLSDKQLEDGLTEEKAA
                     EIVYKKRTREIKRWGLEGFRRDTFYANVLDTIFGKNYPIDSLRYVPYARNNEQFELRA
                     GDITTTSGLIVRVFEARTPYEVFMAGMDKQEVINMRLKAEKYDKYPGLMVGNIFEANN
                     NAGNWE"
     gene            1363..1968
                     /locus_tag="PKY68_07865"
     CDS             1363..1968
                     /locus_tag="PKY68_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOH24984.1"
                     /translation="METYCKNNSLQSTRDTGSFILEYASNFLILPKELDPAYYFKFHF
                     PEISGPTIQTLMLSDQKQQVVFELPQKHSELYQNYFPQTPLLCLPLCFAEFVMGHSSG
                     RTHCLFYLHQNLLMVFAARRGVLSYANRFDVVTDDAAAYFILSIRQVFETGGHCSLCT
                     EKEPAGALQALLEPHFEQIDAYLLDRDLHLFISTLSGQIKL"
     gene            1965..2507
                     /locus_tag="PKY68_07870"
     CDS             1965..2507
                     /locus_tag="PKY68_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOH24985.1"
                     /translation="MRIIRGTYKGRRFEIPHTLKARPTTDFAKEALFNILDNRLNYEE
                     LSVLDLFSGTGSIGLEFVSRGARTVYCIEQYAVHTRFIRSVVEKLGIQNLRLIQGDAY
                     QYIHKHPQVFDVIFADAPYNDPRLMELPDLIFEAGLLKPGGLLVVEHSKLQHFDMHPH
                     WEETRVYGSVRFSFFSQKKP"
     gene            2551..>3200
                     /locus_tag="PKY68_07875"
     CDS             2551..>3200
                     /locus_tag="PKY68_07875"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOH24986.1"
                     /translation="MTKILTIRHQAELENSLNELLSDYEARQIFVLCDHNSKTHCLPL
                     IANFPCLKEAQHIIVPAGDEHKNLETLSAVWSSLSRKGGTRKSLLINLGGGMLSDLGG
                     FAAACFKRGIDFINIPTTLLACVDAALGGKTGINFEGLKNEIGTFYPAKAVLIYSEFF
                     RSLDRDNLYSGYAEMLKHGLLSSHAHWQQVLSFDPATASTRESAGLILDSMTVKEK"
BASE COUNT          867 a          768 c          719 g          846 t
ORIGIN      
        1 acagcgcttt cccgaataca catacctcaa taaatcatcc agaagacgac ccgacccaaa
       61 gaagctgcct tcgaaattct cgttggatat catcgaggct tcgtctacga aaaaaaaagt
      121 attttcgtgc agattgtcgt tcaaatcaaa ccgcccgtag gtgccgtcga cctctttttg
      181 tcgataaatt tttttatgta tagtggtggc agcatgtccg ctgtaagagg caaaaacttt
      241 agccgctctg ccggtagggg ccatcagcac gcaaggctgc ctgttttcgg tcagaaagcg
      301 caccactgca ctgatgagcg cggtcttacc tgttcctgca taacctttga gcaaaagaac
      361 ttccatctcg cccgaaaaca tgaaaaaatt taaacacgaa tcgagcgctt tatgctggtc
      421 ggaagtaggc gtgtaaggaa aatatgcaag aatttttgct cttaaatcca ttaaaataaa
      481 tttctgcggt cattttttag gccgtaaaag tagcgatatt caaattcttg tactaatttt
      541 gtccacatta aaaatgtgaa gttcagtaca caccattaaa ttatcatccc atgagattat
      601 tctttagaat tttgcttatt attgccgtcg gattgtcggt ttatctttgc gtggacagta
      661 tcttacgccc tattcatttt aacagggaaa aagatattcg cttcaatgcc gtaattggtc
      721 gtttgataga tatacggaaa gcccaggtgg aattcagaaa ctatcacggt ctctacgcca
      781 acgatttcga tacgctgatc ggctttgtaa aacacggtta tatccctttt gtattaaaag
      841 aaggcgtgtt gagtgataaa caactcgaag atgggctgac cgaagaaaaa gcagccgaaa
      901 ttgtctataa aaaacgcaca agagagatta agagatgggg gctggaaggt ttccggcgcg
      961 acacattcta tgccaacgtg ctggatacga tttttgggaa aaattacccc atcgactcct
     1021 tacgttatgt cccctatgcc cgcaacaacg agcaattcga gctcagggcc ggtgatatta
     1081 ccactacttc gggtttgata gtccgggtgt ttgaagccag aacaccttac gaagtcttca
     1141 tggccggtat ggacaaacaa gaagtcatca acatgcggct taaggctgaa aaatacgaca
     1201 aataccccgg cttgatggtg gggaacatat ttgaggcaaa caacaatgcc ggtaactggg
     1261 agtaacacga caactgcatc gtttccaact atatccatcc ggtatacgcc gggtggattt
     1321 tcttttctgg aaaaaccttc ggaactcttt tccgcggagg cattggaaac gtattgcaaa
     1381 aacaactctc tacaatctac ccgggatacc ggctcgttca tcctggagta cgcttccaat
     1441 ttcctgattt tacccaaaga gctggatccc gcttattatt ttaaatttca ttttcctgaa
     1501 atcagcgggc caaccattca gactttaatg ctgagtgatc aaaaacaaca ggtggttttt
     1561 gagttgccgc aaaaacacag cgaactttac caaaattact tcccgcaaac tcctctactt
     1621 tgcctgcctt tgtgctttgc cgaatttgtc atgggtcaca gctccggacg gactcattgt
     1681 ctgttttacc tccatcagaa tctgttgatg gtttttgccg cccgcagagg ggtgctcagc
     1741 tacgccaacc gttttgacgt cgttacggat gatgcggccg cctatttcat tttaagcata
     1801 cgacaggtct tcgaaacagg cggtcactgc agcctttgca ccgaaaagga accggccggg
     1861 gcattgcagg ctttacttga acctcatttt gagcaaatcg atgcctatct tctggacaga
     1921 gatttgcact tgtttatatc cacactgagc ggacaaatta agctatgagg atcattagag
     1981 gcacatacaa gggcaggcgt ttcgaaatac ctcatacgct aaaggccaga ccgacgaccg
     2041 actttgccaa agaggctttg ttcaatatcc tggacaaccg cctgaactac gaggagttgt
     2101 cggtgctgga cttattttcg gggaccggca gcatcggact ggaatttgtt tctcgcggcg
     2161 cccgcacagt ttactgcatt gaacaatacg cagttcacac gaggtttata cgctcggttg
     2221 tcgaaaaact tgggatacaa aatctcagat tgatccaggg agatgcttac cagtacattc
     2281 acaagcatcc gcaggtattt gatgtgatct ttgccgatgc tccgtacaac gatcccaggc
     2341 ttatggaatt gcccgatctg atttttgagg ccggcctact caagccggga ggtctgcttg
     2401 ttgtggaaca ctcaaaattg cagcatttcg acatgcatcc ccattgggaa gaaactagag
     2461 tgtacggcag cgtgcgtttt agtttcttca gtcaaaaaaa accatagccg aaacttttta
     2521 taggtttgcg gccgtaaaac agccttctgc atgacaaaaa tactgaccat ccgccatcaa
     2581 gccgaactgg aaaacagcct gaatgaatta ctctcggatt acgaagcccg ccagattttt
     2641 gttttatgcg accacaacag taaaacacat tgcctgcctc tgatcgcaaa ttttccctgc
     2701 cttaaagagg ctcaacacat tattgtaccg gccggtgacg aacataaaaa cctggagacc
     2761 ttgtctgctg tctggtcttc gctcagccgt aaaggcggaa cccgcaaatc gctgctcatc
     2821 aacctgggcg gaggtatgct gagcgatctg ggcggctttg cggctgcctg tttcaaaaga
     2881 ggcattgact tcatcaacat acccaccacc ctattagcct gtgtagacgc agctctggga
     2941 ggcaaaaccg gcatcaactt cgagggcctg aaaaatgaaa tcggcacctt ttatccggcc
     3001 aaggccgtat tgatttactc ggaattcttc cgcagcctcg accgggacaa tttgtattcg
     3061 ggctatgcgg aaatgctcaa acacgggctc ttgtcgtccc atgctcattg gcagcaggtg
     3121 ttgagctttg atccggccac ggcctccact cgtgaaagtg ccgggcttat tctggattcc
     3181 atgacggtca aagaaaaaat
//
LOCUS       DAOSGL010000267         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28538_length_3187_cov_3.455939, whole genome shotgun sequence.
ACCESSION   DAOSGL010000267 DAOSGL010000000
VERSION     DAOSGL010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28538_length_3187_cov_3.455939"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..641)
                     /locus_tag="PKY68_07880"
     CDS             complement(15..641)
                     /locus_tag="PKY68_07880"
                     /inference="COORDINATES: protein motif:HMM:NF027202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BF3164 family lipoprotein"
                     /protein_id="HOH24987.1"
                     /translation="MMKNLIYLLPLLLLFSCREKSVTTITVAGFPSTIELEAKTLETD
                     TAILAPSEMFILNDQLWILQSRNNPLFWVYSLDKGNFLYSTGYKGHSSNEFIHPGFMT
                     IQLINEHTFTMLDNSFLKTIEVSKDGILNVLKTESIFDLRPINGFVRLNDSLYCAFAD
                     CATGTESDFEYRIKNRNTNKESKFSEYPVLFNFEEKAMKDVSCIINIW"
     gene            complement(694..996)
                     /locus_tag="PKY68_07885"
     CDS             complement(694..996)
                     /locus_tag="PKY68_07885"
                     /inference="COORDINATES: protein motif:HMM:NF025421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NVEALA domain-containing protein"
                     /protein_id="HOH24988.1"
                     /translation="MKKTIFLSIALVCFALGTSLLINSEAKKHINPLAFANIEALTED
                     EPGLNEITCYSTFRITSNDPEEDVWVITDCNGCEEVNVFEYRDKGTCTKKTGAIFV"
     gene            complement(1362..>3187)
                     /locus_tag="PKY68_07890"
     CDS             complement(1362..>3187)
                     /locus_tag="PKY68_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH24989.1"
                     /translation="LFLSCQYSGPVEEALRQSGRNRSELERVLDFYREKGDDLSLQAA
                     KFLISEMPGHHSLENPSLSEFRQALDTMYPQMSNVIKRVVYQVPYRKDEFVANAEKRY
                     DLETLSADYLIAHIDNIVEMWRKCSWLKDYSFEEFCEYLLPYRFAREPLLTRFDSTTY
                     WWQEIKARTEYYKHLPPSPMELRHFLHGIIDRQDDPYMQDFELPMSKGKHTFDCLDKC
                     YYNLSTLRSAGIPCAIDYVPAWPTRNGTHYWWVLIDPLCMHNTYSDTQNPRAAKVLRQ
                     TYSHHPVPRPGKNEYIPEQFLNPFNKDVTAEYVKVSDVKLSFPLFRQRHAYLAVFNEM
                     SWKPVAWAKIRGGRALFKEMGRSVVYLPVCYKKEQLCSAGNPILLKSDGSTVELNPKS
                     ERFSLTLTRKYPLTYSKTQWSRNMLDCRFEAGDDSTFRDSTVVFHISRPDPNLNYVEV
                     FVPDSIGAFRCWRIIGPKRIWLGELAFYSKEGEKLSGRTIYHPEDKGETPENAFDNDE
                     LTYTNVYAWLGKDFERPEKVSKIRYISRTDANGIIPGMEYELMYFDRQWISAGRQTAV
                     ADSLVFDSLPAGALFWLRNLTEGREERIFTYDSEFDRIVYW"
BASE COUNT          907 a          685 c          630 g          965 t
ORIGIN      
        1 ctgtttagca ttagctacca gatatttata atacaactga catctttcat cgccttttct
       61 tcaaagttga ataacaccgg atattcgctg aatttgctct ccttgtttgt atttcgattc
      121 tttatgcgat actcaaaatc gctctcagta cccgttgcac agtcagcaaa agcacagtat
      181 aatgaatcgt tcaaacggac aaaaccattg atcggacgca gatcaaagat gctttccgtt
      241 ttaagcacat tcaagatacc gtcttttgac acttcgatgg ttttcagaaa ggaattgtcc
      301 aacatggtaa aagtatgctc gttgataagt tgtatagtca tgaaaccggg atgaatgaat
      361 tcgttggagg aatggccctt gtaaccggtg ctatacaaaa aattaccttt atccaggctg
      421 tagacccaaa acaatggatt attacggctt tgtaaaatcc acaattggtc gttgagtata
      481 aacatttcac ttggagccag aatggctgta tccgtctcca gggttttggc ttccaattca
      541 atagttgatg ggaaaccggc cactgttatt gttgttaccg atttttcccg acaggaaaaa
      601 agcagtaata gaggtagtaa gtaaatcaag ttcttcatca taataatttt ttatgtaata
      661 cgcagaaaat attgtctgcg tattacgtta cttttatacg aatatagcgc cggtcttttt
      721 agtgcaagtt cccttgtctc tatattcaaa tacatttact tcttcacatc cgttacaatc
      781 agtaattacc caaacatctt cttcaggatc gttagatgta attctaaaag tactgtaaca
      841 ggtaatctca tttaaacctg gttcatcttc tgtcaatgct tcaatatttg cgaatgctaa
      901 tggattaata tgtttcttcg cttctgagtt aattaataaa cttgtcccca aagcaaagca
      961 aacaagtgct attgataaaa aaattgtttt tttcatagtt caactaattt aaagatttac
     1021 aaatttaaaa ataagtgtat tggtttaatc aatacttgtg gctgtatcta tcctttcaac
     1081 agcccaaaac cgcgtaggtc ctttaatttt agcttctgcg ctcactttcg ctacattttt
     1141 agggttctcc ttagaatgta gcttcgttcg ggaaaagcaa aattcttttt cttttccgct
     1201 cactttcgct acatttgtta cctcctttct tttttaggag gacgtcgggg agtaaagcct
     1261 ttgcagtttg aacttgtgcg gcgggctttg ctccccttcc ttcctcagag ttatattcta
     1321 aaattagaat tactattatc aaaataaatt ggttaacctc attaccagta cactatccta
     1381 tcaaactcac tatcgtaagt aaagatgcgt tcttctctgc cttcggtcaa attgcgaagc
     1441 caaaacaaag cgccggcggg aagactatca aaaacaagac tgtcggctac agccgtctgt
     1501 cggccggcag aaatccattg tcggtcaaaa tacatcaact cgtattccat ccccggaata
     1561 ataccattgg catcggtacg ggagatataa cgaatcttac tcactttttc gggtcgttca
     1621 aaatctttac ccagccaggc atatacgttg gtatatgtca actcatcatt atcgaaagca
     1681 ttttcgggag tttctccttt atcttcggga tgatatatgg tacgacctga aagtttctcg
     1741 ccctctttgc tgtaaaaggc taattcgccc aaccatatcc gtttggggcc aatgatacgc
     1801 caacagcgaa aagctccaat actgtcgggc acaaacactt ctacatagtt cagattaggg
     1861 tccggtcggg aaatatgaaa gaccacagtc gagtccctga atgtagaatc gtcgccggct
     1921 tcgaaacgac aatcaagcat attgcgactc cattgtgtct tggaataagt caacggatat
     1981 ttacgtgtca gcgtcagaga gaaacgctct gattttggat ttaattccac cgtgcttccg
     2041 tcgcttttca agagaatagg attgcctgca ctgcacagtt gctctttttt ataacagaca
     2101 ggcaggtaaa ctacactacg ccccatctcc ttaaacaagg ctcttcctcc tctgattttt
     2161 gcccaggcaa cgggtttcca gctcatttcg ttgaatacgg ccagataggc atgcctttgc
     2221 cgaaataaag gaaatgaaag ctttacatcg gaaaccttta catattcggc tgtgacatcc
     2281 ttgttaaacg ggttgagaaa ttgttcaggg atatattcat tctttcccgg acgcggtaca
     2341 ggatgatgtg aataggtttg tcgaagcacc ttggcagcac gagggttttg ggtatcgcta
     2401 taggtattat gcatacacaa gggatcaatc aacacccacc aataatgagt tccgttgcgg
     2461 gtaggccacg cgggaacgta gtcaatggca cagggtatgc cggcagaacg gagagtggac
     2521 agattgtaat aacacttgtc aagacaatcg aaagtatgtt ttcccttgga cattggaagt
     2581 tcaaaatcct gcatataggg gtcgtcctgg cgatcgataa tgccgtgcag gaagtggcgc
     2641 agttccatag gagatggagg aagatgttta taatattcgg tccgggcctt tatttcctgc
     2701 caccagtagg tggtggaatc gaaccgtgtt aatagcggtt cgcgagcaaa ccgataaggc
     2761 aacaggtatt cgcaaaattc ttcaaaacta taatccttca accatgaaca tttgcgccac
     2821 atttctacta tattgtcgat atgagcaatc agataatcag cgcttagggt ttcaagatcg
     2881 tagcgttttt ccgcattagc gacaaattca tccttgcgat agggtacttg atagacgacc
     2941 ctttttatta cattcgacat ttggggatac atagtatcca aagcctgtct aaattcagac
     3001 aaagaaggat tttccaaact gtgatgccct ggcatttcag aaataagaaa ttttgcagct
     3061 tgtaacgata aatcatcgcc tttttctcta taaaaatcca gtaccctttc taattcgctg
     3121 cgattcctgc cactttgccg caaggcctcc tctactggcc cggagtattg gcacgacaga
     3181 aaaagaa
//
LOCUS       DAOSGL010000268         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28592_length_3182_cov_3.789894, whole genome shotgun sequence.
ACCESSION   DAOSGL010000268 DAOSGL010000000
VERSION     DAOSGL010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28592_length_3182_cov_3.789894"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PKY68_07895"
     CDS             <1..1034
                     /locus_tag="PKY68_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24990.1"
                     /translation="NDLKSEFTDRQLLNNWLGYHCLPGRIYIADVLLSTTLNTSVKGQ
                     PITITLVDEHLYLNRFKTERIDEDGIFMDRQGVYVDYNCSNGVVQTIDDMLEIIQRSA
                     YRVYWDMGDQPEIKALKGYRKAGTKVTYLPGELSMMTWGGSNNPTVTYSAWGLPDING
                     AYDQKSQYVYNDYLTFRVCTRVMQWLEIKTPVLVAGKYHVWVCWRRMNPCKFRTTFKQ
                     EGEEDQVFPAVVDLQDYMPVELDDDQLEAKGWKQYNAKFRISVFNSKNVATIDVKTTG
                     SHILRFDALVGSKDVGNNWDMIQFIPINDDQVWPMIAMDGTLVAKGTPDCQIFPEDGS
                     NCPVEEEEE"
     gene            1056..2828
                     /locus_tag="PKY68_07900"
     CDS             1056..2828
                     /locus_tag="PKY68_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH24991.1"
                     /translation="MKYLSVKSLGLILLSTVLLGCADQWAEHIRPADKNLETSLFQAI
                     KAEQELSLFQAFLQETGYDAVLESTENYTVFAPLNTALSAYQSASVEVKKNLVKNHIA
                     ALSYNTADLNSMEYLMMLSGKRLRLDELSLNAKETVCSNGILRTSNSISLPKANLYEL
                     ISAYKDSLEIARAIFLAGDSIMDPVKSIQTGVDPHSGRPIYDTAYLYYNPYLESLSIH
                     QEDSSYCFVLLNNSNFADLKAKYAKYMQQWDESIRAVNDSLTDLVVTRELINDLICPT
                     EGFEESAPTYLNTKALSLDMGKALIMDRLEASNGLLLICDSVNIRMKDNKIKELYIQG
                     EDYVETLNPAYTYVRLRDYAMGGKDVMVAGRWSESFDYVTVLDGVDTTLSKTVNYYYY
                     SATASGSYYDKSINFYLKYTPTLYSTKYRLYWKTYDDFGRHFAGDTLDVLFNPEDPNQ
                     ACPSVYRVTQKLYISLPGRPELKRNNGIIENLSLQSGAKVMVGITPDTTDASGRGHTI
                     GVNAGVETEMPLRWYKAAGDYEALSTPLAAANTRDYLNCTSMGTACFYVTNSPYTSAS
                     NSTSNGLIFLDYIRLVPEMIDEND"
     gene            2821..>3182
                     /locus_tag="PKY68_07905"
     CDS             2821..>3182
                     /locus_tag="PKY68_07905"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOH24992.1"
                     /translation="MISEYSSSRTMIKCLKTIGILLLLTGIAVLPVKAQDGLVTVTGK
                     VLSQADQKGLPGISVTVQNLTSVMTADDGGFSLSLPSADAELSISGPGYQTKKVALRG
                     RTQLIVKLHADNFTNSVY"
BASE COUNT          871 a          724 c          780 g          807 t
ORIGIN      
        1 aaaacgacct gaaatcggaa tttaccgacc ggcaattgct gaacaactgg ctgggttatc
       61 attgtctgcc gggcaggatt tacattgccg acgtattgtt gtctaccacg ctgaatactt
      121 ccgtcaaagg tcagcctatt accatcacct tagtcgatga acacctctat ttgaatcgct
      181 tcaaaacgga aagaatcgac gaagacggta tttttatgga cagacagggt gtttatgtgg
      241 attacaattg ttcgaacgga gtggtgcaaa ccatcgatga catgctcgaa attatacaac
      301 ggtcggccta tcgtgtgtac tgggatatgg gagatcagcc cgagataaag gcactcaaag
      361 gttaccgtaa agccggcacc aaagtgacgt atctgccggg agaattgtct atgatgacct
      421 ggggcggaag caataatccc acggtcacct attccgcctg gggtttgccc gacatcaacg
      481 gagcctacga tcaaaaatcg cagtatgtgt acaacgatta tcttactttc agggtgtgta
      541 ccagggtgat gcaatggctg gaaatcaaga ctccggtgct ggtggccggc aaatatcacg
      601 tttgggtttg ctggaggcgt atgaatccct gtaagttcag aaccactttt aaacaggagg
      661 gcgaagaaga tcaagtgttt ccggccgttg tagacctgca ggattatatg ccggtggaac
      721 tggacgacga ccagttggag gccaaaggct ggaagcaata caacgctaaa ttcagaatca
      781 gcgtcttcaa tagtaaaaat gtggccacca tagacgtaaa aacgaccgga tcgcatattt
      841 tgcgttttga cgctttggta ggctccaaag acgtgggtaa caactgggat atgatacagt
      901 ttattccgat caacgacgat caggtatggc ccatgattgc catggacggt accctggttg
      961 caaaaggcac ccccgattgt cagatctttc ccgaagacgg cagcaattgt ccggtcgaag
     1021 aggaagaaga gtagctaata attaatagaa aaacaatgaa atacctttct gtaaaaagtt
     1081 taggccttat attgttgagc accgtgctgc tgggttgtgc agatcagtgg gctgaacaca
     1141 tccgcccggc ggataaaaat ctggaaactt cgttgttcca ggccataaag gctgaacagg
     1201 agcttagtct ttttcaggct ttcctgcagg aaacaggtta cgatgcggtg ttggaatcaa
     1261 cggaaaacta cacggttttt gcaccgctca atacagcctt atctgcttac caatcggcct
     1321 cggtggaagt aaagaagaat ttggttaaaa accatattgc cgctttgagt tacaacacgg
     1381 cagacttgaa ttctatggag tatttaatga tgctgagtgg caaacgcctc aggctggacg
     1441 aactgtcgct taatgcaaag gagaccgtat gcagcaacgg tattctccgc accagcaata
     1501 gcatcagctt gcccaaggcc aatctttacg aactgatttc agcctataag gacagtttgg
     1561 agattgcccg ggccattttt ctggccggcg attccattat ggatcccgtc aagagcatac
     1621 aaaccggagt agatccccac agcggtagac ctatttacga tacggcttac ctgtattaca
     1681 atccttacct ggaaagttta tcgatccatc aggaggattc ttcgtattgt tttgtattgc
     1741 tcaacaacag caattttgcc gatctcaagg ccaaatacgc caaatacatg cagcaatggg
     1801 acgaaagtat cagagcggtc aacgacagtt tgacggattt ggtggtgaca cgcgaactta
     1861 tcaacgattt gatttgcccc accgaaggtt ttgaagaaag cgccccgacc tatctgaata
     1921 cgaaagcgct gagcttggat atgggcaaag cattgattat ggataggttg gaagcttcca
     1981 acggactgtt gttgatttgc gacagtgtca atatccggat gaaggataac aaaatcaaag
     2041 aactgtatat ccagggcgaa gattacgtgg aaactcttaa tcctgcttac acttacgttc
     2101 gtttgcgcga ttacgccatg ggaggcaagg atgtgatggt cgcaggccgc tggtccgaaa
     2161 gtttcgatta tgtcactgtg ctcgacggtg tggacaccac cttgtctaaa acggtcaact
     2221 attattatta cagcgccacg gcttcgggct cttattacga taagtccatc aatttttacc
     2281 tcaagtacac acctacgctc tattcgacta aataccggct ttattggaaa acttacgacg
     2341 attttggacg tcattttgcc ggtgatactc tggatgtgct ttttaatccc gaagatccca
     2401 atcaggcttg cccctcggtg taccgggtta cccaaaagct ttacatttcg ttgccgggca
     2461 ggccggaatt gaagcggaac aacggtatca ttgaaaactt gtcgcttcag tcgggagcca
     2521 aggtaatggt gggcattacc cccgatacga ccgatgcatc aggacgtggt cacaccatag
     2581 gcgtaaatgc cggagtcgaa accgaaatgc ctctgcgttg gtacaaagcc gccggagatt
     2641 acgaagcgct gtccactcct ctggccgctg ccaatacccg cgattacctg aattgcactt
     2701 cgatgggaac agcctgtttc tatgttacca attctcccta taccagtgct tctaatagca
     2761 cttccaacgg tttgatcttt ttggattata tccgtttggt acctgaaatg atcgatgaaa
     2821 atgattagcg aatactcaag ctcaagaacc atgataaaat gcttaaaaac catagggata
     2881 ctcctgctgt tgacagggat tgccgtttta ccggtcaagg ctcaggacgg gcttgtgact
     2941 gttaccggta aggtgttgtc tcaagccgat caaaaaggct tacccggtat cagcgtaact
     3001 gtgcagaacc tcacctctgt tatgacggca gacgatggcg gttttagcct gagcctgcct
     3061 tcggccgacg cagaacttag tatctccggc ccgggttatc agactaagaa agtagcgctg
     3121 aggggcagaa ctcaattgat agtgaaatta catgcggata atttcaccaa ttccgtctac
     3181 aa
//
LOCUS       DAOSGL010000269         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28712_length_3172_cov_4.113250, whole genome shotgun sequence.
ACCESSION   DAOSGL010000269 DAOSGL010000000
VERSION     DAOSGL010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28712_length_3172_cov_4.113250"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2158
                     /locus_tag="PKY68_07910"
     CDS             <1..2158
                     /locus_tag="PKY68_07910"
                     /inference="COORDINATES: protein motif:HMM:NF027701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fasciclin"
                     /protein_id="HOH24993.1"
                     /translation="NYTMFLRGVDLAGYAPIVKGKSILTVMAPNDSAFTAYLQKAGYA
                     GIDQMHAENPEQLSKLIGFHLLYYAFNWDNLVNFRPIEGDEASAEALLVNAGCYYKFR
                     TKSADPISMEWDGKDSVRVYHFERFIPVFNTQLFATRGIDPKYNYEYFFPNTEWTGSR
                     HTGKIDGFNVSNASVIDDDHVITDNGYLYHISQVLDPLETIYTELKNNADYSIFFELF
                     NSYGEYQEEPELTTDFGNGQTLYLHTHPGLVNIACEWPVTDYTAMDRLSSVGYNLFAP
                     SNDAVQNFFTTYWTPGCGYSNLNELDPLILQYFISQTFGSDDVVAFPEEIKKGLIKTV
                     YGTPVNIDPDQVTWRQMCCNGALYGMDRMTPPAIFSSVIGSAFRDKRFLPYLYALDGS
                     DLVLALASQNTEFVALIPDTAQFRGAAIRFETIAGGRHELQVIGDDGNYAAMSVGTMR
                     DMVNMNVSSSVNQLSTTGFQVVETNTAFNYWYLYDGKITTSALFNKQLNPTYVDEIYF
                     DFAPLEMNPAQPNGGKWDNGSVYTYTAPSIFMPGEDEGLNRTLAVCNDNTYPYYLFSQ
                     LLSKAGLVEEQQLKIVMDDARFIAFVPTNEAIKARITEIPGCDKCGVSDDMVLTGNPN
                     KALLANYLRSYFIISDLTPFSVYPYPGSGMKGSFDTSGAYKLNITDTGTTLTVSLTDK
                     DGIKGAEVSLHNTEKFHSLPFSYSDGAFHLINDILL"
     gene            2169..>3172
                     /locus_tag="PKY68_07915"
     CDS             2169..>3172
                     /locus_tag="PKY68_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOH24994.1"
                     /translation="MKQIKIFLIVLVAVLVWPQLQAQNTVVTGTVTELYDGKVEPVYN
                     ANVVVVSSNNRYITGAITNIDGQYSIVVPAYEKEPRIRFSYIGLKTQTIDYTGQKVLN
                     VRMEPDSKSLDEVQVTGRRVDRDELGISFTEQTSSTQKIEMSDIVQSTPISDITEALQ
                     GQLAGVDIVVGGDPGARSAIRIRGVNSLNASSEPLIVVNGIPYHVDIADDFNFATANE
                     EDFGALLNIAPYDIESIEVLKDASATAIYGTKGANGVLLITTKRGSKGKTTFGFNSKF
                     TTKFEPDPIPLLDGNQYVALMQDAIWNAANAKGLTSARNELEQLFNTYDINYVRDWQY
                     "
BASE COUNT          838 a          743 c          751 g          840 t
ORIGIN      
        1 caactacaca atgtttttgc gcggtgtaga tttggcgggt tacgctccca ttgtaaaagg
       61 caagagcatc ctgacggtga tggcccccaa cgattctgcc ttcacggcct atctgcaaaa
      121 agcaggttat gccggcatcg accaaatgca tgcagagaac ccggaacagc tttctaagtt
      181 gataggattt catttgcttt attatgcatt caactgggat aatttagtta atttccgtcc
      241 gatagaaggc gacgaagcct ccgccgaagc cttgttggtg aatgcgggct gctattacaa
      301 gtttcgcacc aaaagcgccg accccatttc tatggaatgg gacggaaaag actctgtacg
      361 cgtatatcat tttgaacggt ttattccagt tttcaacaca cagttgtttg ctaccagagg
      421 catcgacccc aaatacaact acgagtactt tttccccaat accgagtgga cgggcagccg
      481 tcacacagga aagatcgatg gtttcaatgt gtcgaacgcg tcagtgatag acgacgatca
      541 tgtcattacc gataacggtt atctttacca tatcagtcag gtgttggatc cgctcgaaac
      601 catatacacc gagttgaaaa acaacgccga ttattccatc tttttcgagt tgttcaattc
      661 ttacggtgag tatcaggaag aacccgaact gactaccgat tttggcaatg gacaaacctt
      721 gtatctgcac actcatcccg gtttagtgaa catagcctgc gagtggccgg tgaccgatta
      781 tactgccatg gacaggttgt cgagtgtggg atataatttg tttgcgccct ccaacgatgc
      841 tgttcagaac tttttcacta cttattggac gcccggttgc ggttacagta atctgaacga
      901 attagatccg cttattttgc aatactttat cagccagacc tttggaagtg acgacgtggt
      961 agcctttccc gaagaaatca agaagggttt gatcaagacc gtttacggca cgcccgtcaa
     1021 catcgatccc gaccaggtaa cctggaggca aatgtgctgc aacggtgctc tgtacggaat
     1081 ggaccggatg acgcctcccg ccattttctc ttcggtgata ggcagtgctt ttcgtgataa
     1141 acgattctta ccttatttat atgctttaga cggctcggat ttggtgttgg cattggcttc
     1201 tcaaaacacc gaatttgtgg ctttgattcc cgatacggct cagttccgcg gtgccgccat
     1261 acgtttcgaa acgatagcgg gcggtcgtca tgaattgcaa gtgattggcg acgacggcaa
     1321 ttatgccgcc atgagcgttg gaaccatgcg cgatatggtt aatatgaacg tgtcatcttc
     1381 ggtaaaccaa ctatcgacta ccggttttca ggtggtagaa accaatacgg cattcaacta
     1441 ttggtatctg tacgatggca aaattaccac cagtgccttg tttaataaac agttgaatcc
     1501 cacctacgtc gacgaaattt acttcgattt tgctcccctc gaaatgaacc cagctcagcc
     1561 caacgggggc aaatgggaca acggaagcgt ttacacctac actgctccga gtatttttat
     1621 gccgggcgag gacgaaggcc tgaaccgcac ccttgcggtt tgcaacgaca acacttatcc
     1681 ttattatttg ttctcacaat tgctcagcaa ggctggattg gtcgaggaac aacaattaaa
     1741 aatagtgatg gatgatgctc gtttcatagc ttttgtgcct accaacgaag ccatcaaagc
     1801 gcgtatcaca gaaatccccg gttgcgacaa atgcggcgtt tccgacgaca tggtgctgac
     1861 cggtaatccc aacaaagcgt tgctggccaa ttatcttcgc agttatttta tcatcagcga
     1921 tttgactcct ttcagcgttt atccttatcc gggcagcggc atgaaaggca gtttcgatac
     1981 atcgggagct tacaaactca atatcaccga tacgggtacg acgctcaccg ttagtctgac
     2041 cgacaaagat ggcataaaag gagcggaagt gtctttgcac aataccgaaa aattccattc
     2101 cttgcctttc tcgtacagcg acggtgcttt ccatttaatc aacgacatct tgttataaat
     2161 tggccattat gaaacaaata aagatatttt tgatagtgtt ggtcgctgta ttggtttggc
     2221 cgcagttaca agctcaaaat acggtggtga cgggtacggt caccgaattg tacgacggca
     2281 aagtagagcc tgtctataat gcgaatgtcg tggtggtcag ttcgaataac cgttatataa
     2341 caggtgccat taccaatatc gacggacaat acagcatcgt tgttcctgct tacgaaaagg
     2401 aaccgcgcat tcgattctcc tacatcggcc tgaaaaccca aacgatcgac tatacgggac
     2461 aaaaggtttt aaatgtgcgt atggaacccg acagcaaaag tctggacgaa gtacaagtga
     2521 cgggcaggag agtagacaga gacgaattgg gtatttcgtt cacggagcag acttcttcga
     2581 cgcaaaaaat tgaaatgtcg gatattgtgc agagtacgcc tatctccgat atcaccgaag
     2641 ccctgcaagg ccagttggcg ggtgtggata tcgtggtggg cggcgacccg ggagcgcgca
     2701 gtgccatccg tattcgtggt gtcaattcat tgaacgcttc gtcggagccg ctcattgtgg
     2761 tcaacggtat tccttatcac gtcgatatcg ccgacgattt caattttgcc acggccaacg
     2821 aagaagactt tggcgccttg ctcaacattg caccctacga catcgaatcg atcgaggtat
     2881 tgaaagatgc ttctgctaca gctatttatg gtactaaggg tgccaatggt gtcttgttga
     2941 ttaccaccaa aagaggttca aaaggcaaga ccacttttgg tttcaacagc aagtttacca
     3001 ccaagtttga acccgatccc attcctttgc tcgatggtaa tcagtatgtg gctttgatgc
     3061 aggatgccat ttggaatgcc gctaatgcca agggtttgac atcggccaga aacgaattgg
     3121 aacaattgtt caatacctac gatatcaatt atgtcaggga ctggcagtat tt
//
LOCUS       DAOSGL010000270         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28725_length_3171_cov_4.843068, whole genome shotgun sequence.
ACCESSION   DAOSGL010000270 DAOSGL010000000
VERSION     DAOSGL010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28725_length_3171_cov_4.843068"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PKY68_07920"
     CDS             complement(<1..1054)
                     /locus_tag="PKY68_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOH24995.1"
                     /translation="MKSIIRKSIVLLLITAGGWYSATAQNLTVKITKTDNSEEILTAQ
                     GSLEAALSGVPPDSVASLEIRGGDFVAADWLWLLEQGYRLDKMTSFCITNEVNSVADI
                     PNTSYLAPYFSHSVETLSVAKVKRIGNYAFEGTSLTSVSFPHATDVGERVFRECRQLA
                     NVSLPMLTELKEYTFLECKALTTISLPSLVSTGNYDFKDCINLTSVDMPVLTTLGYET
                     FIDCSSLTDVNLPELTDIGNNAFSGCVALSSISFPKLKNVRSFSGCIALQSADLPLAE
                     TIESYAFGGCTALTGITAPRLTFVGDAAFEGCIRLGNLRLGATPPGAYTSSFAGCLSP
                     RFLQPVDDNGMPLTGSE"
     gene            complement(1090..2703)
                     /locus_tag="PKY68_07925"
     CDS             complement(1090..2703)
                     /locus_tag="PKY68_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH24996.1"
                     /translation="MKNNRDLLFLKFIGILFCTTLASCSLDFVRSNNIKNDSFWDTKD
                     GKAIYSQGGGIFKFTHPETGEEKYFWYGVHYKQAETYREDPSSTMQGNTFEGVTCYSS
                     TDLVNWEFEANVLTPEEVFGDSGRRWGWLGRMGVAYVKEAKQYVLIIQYNSSVLFATA
                     DSPLGPFKKQHRKDMTSWIGTPNTGDQTVFTDEDSGKSYLVYSYGQGRNKIYLSEIGL
                     KNDTITLLDFKQVFRGAGREGNCMFKYRGKYYIAASQLYGWDGSLAYYLVADDIMGPY
                     LPENEMQVIPGVEDDYGHISQTGFFVTVRGSKQETVIFCGDRWANFAGNGLGYNQWCP
                     LSFDGYVPYFNSLNSWNLNVETGEWEVAGDNNWVKNASFEADRKQMPSPVKPVQTRLL
                     GWETEVIKGTEISLDSLSPILNHENTEEERKIVVGERSLNMSDRVDFVRKVSQKITSS
                     PYVTFPNASYTLTAKIKNSGGFNKLEMYAESGGKRYSVRVKEENETWTSIGIEKLKVK
                     NNAVEIGFFADGQAGAFCLVDDVSLVKDR"
     gene            complement(2724..>3171)
                     /locus_tag="PKY68_07930"
     CDS             complement(2724..>3171)
                     /locus_tag="PKY68_07930"
                     /inference="COORDINATES: protein motif:HMM:NF037850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HOH24997.1"
                     /translation="YRLMWHDVVYEAGELKAVAYRAGKVIGEKVLRTAGEAYTLKLTP
                     DRSRLKASGEDLSFILVEACDKNGNLCPLADYMINFELKGPGEIAGVGNGNPQSHEPF
                     VAPYRKLFNGKAMLIVRTTANKPGKITVEASAEGLKPAKTSLTSEQ"
BASE COUNT          768 a          788 c          736 g          879 t
ORIGIN      
        1 gctctgaacc cgtgagcggc atgccgttgt catctacagg ttgcaggaag cgagggctga
       61 gacaaccggc gaaggaactg gtgtacgcgc cgggcggagt tgcgcccaaa cgcaggttac
      121 ctaaccttat acaaccttcg aaagccgcat caccgacaaa agtaagcctg ggggctgtga
      181 tgccggtaag ggcggtgcaa ccaccgaaag cataactctc gattgtttct gccaaaggta
      241 ggtctgcact ctgcaacgca atacaaccac tgaagctacg cacatttttc aacttaggga
      301 agctgatgct cgagagtgct acgcagccac taaaagcatt attcccgata tcggtgagct
      361 caggtaggtt tacatcggtc agggaactgc agtcgataaa ggtttcatag cccaaggttg
      421 ttaagacggg catgtccacg gaagtcaggt tgatgcaatc cttaaagtcg tagtttccgg
      481 tgctgaccaa cgagggtaat gaaatggtgg taagggcttt acactccaaa aaggtgtatt
      541 cttttaattc cgtcagcatg gggagactca cattagcaag ctgtctgcac tcacggaata
      601 cccgttctcc aacatcggta gcatggggga agcttacgga ggtaagactc gttccctcaa
      661 aagcgtaatt gccaatgcgc ttcaccttcg ccacactaag ggtctccact gaatgggaaa
      721 agtagggggc aaggtagctt gtgttgggaa tgtcagccac gctattcacc tcgttggtaa
      781 tacagaagct agtcattttg tccagcctgt acccctgttc gagcagccag agccagtctg
      841 ccgccacaaa gtcgccgccc cttatctcta acgaggccac actatccggc ggcacaccgc
      901 taagagcggc ttcaagactt ccctgagcgg ttagtatttc ctcgctgttg tcagttttag
      961 tgatttttac cgtcaaattc tgcgctgtgg cagagtacca gcctcctgca gtaataagca
     1021 gcagaacaat ggatttgcga attatggact tcatgatatg tgagaattaa ttgttgacca
     1081 caaggtacac tacctgtctt ttaccagaga cacatcgtcg accagacaaa aggctccggc
     1141 ctgaccatcg gcgaagaaac cgatttcgac cgcattgttt tttactttta acttttcaat
     1201 gccaatgctt gtccatgttt cgttttcttc tttcactctt acggagtatc gtttgccgcc
     1261 cgattcggca tacatttcga gcttgttgaa gcccccactg tttttaattt tggctgtcag
     1321 cgtatagctg gcgttgggga atgttacgta gggagaggaa gtgattttct gagatacttt
     1381 ccggacaaaa tctactctgt cgctcatgtt cagactgcgt tcgccaacaa ctattttacg
     1441 ttcttcttcg gtgttttcat ggtttagaat gggcgaaagc gagtcgagcg aaatctcggt
     1501 tcctttgatg acctcggttt cccaacccag cagtctggtt tgtaccggtt tcaccgggct
     1561 gggcatttgt ttcctgtcgg cttcaaaact ggcgtttttt acccagttgt tgtcgccggc
     1621 cacttcccat tcgccggttt ctacatttaa attccaagaa ttgagcgagt taaaataggg
     1681 aacatagcca tcgaacgaca aggggcacca ttggttgtat cccagaccgt ttccggcaaa
     1741 attggcccag cggtcgccgc agaaaataac cgtctcctgt ttgctgcccc ggaccgtaac
     1801 aaaaaatccg gtttgagaaa tatgaccgta atcatcttca acgccgggaa tcacttgcat
     1861 ctcgttttcg gggaggtacg gccccataat gtcgtccgcc accagataat aggccagtga
     1921 accatcccat ccgtacaatt gcgaggctgc aatgtagtat ttgccccggt acttgaacat
     1981 acagttgcct tcgcgccctg ctccacgaaa tacctgttta aagtccaaca aggtaatggt
     2041 atcgtttttc aagcctattt cggacaaata gattttattg cgtccctgac cgtaactgta
     2101 caccaggtaa gatttgccgc tgtcttcgtc ggtaaatacg gtttggtcgc cggtatttgg
     2161 agtgccgatc cagctggtca tatcttttcg gtgctgtttt ttgaatgggc ctaaaggaga
     2221 atcggcggta gcaaaaagca cgctcgaatt gtattgaatg atcagtacat attgctttgc
     2281 ttcttttaca taggccacgc ccatgcgtcc cagccaaccc catcttcgcc ccgaatcacc
     2341 gaatacctcc tcgggtgtca gcacatttgc ttcaaattcc caattcacca ggtcggtgga
     2401 cgaataacag gttacgcctt cgaaagtatt gccttgcata gtcgatgatg gatcctcccg
     2461 gtaggtttca gcttgtttat aatgtacccc ataccaaaag tatttttctt cgccggtttc
     2521 gggatgtgtg aatttaaaaa tgccacctcc ctggctataa atggctttgc cgtctttggt
     2581 gtcccaaaag ctgtcgtttt tgatgttgtt cgaccggaca aaatctaaac tgcaggaagc
     2641 taaagtcgtg cagaacagga ttccaataaa tttcaaaaaa agtaaatcgc gattgttttt
     2701 catttgcaag aatttataaa atgttattgt tcgctggtca aagaagtctt tgcaggtttc
     2761 aagccttccg cactcgcttc aacagtgatt ttaccgggtt tatttgctgt cgttcttacg
     2821 atcagcatgg cttttccgtt gaatagcttt cggtagggtg ccacaaacgg ttcatgcgat
     2881 tgcggattac cattaccgac accggcaatt tcgcccggac cttttagttc gaagtttatc
     2941 atataatcgg ccagcggaca aagatttccg tttttatcgc aagcttcgac cagaataaaa
     3001 cttaaatcct cgccgcttgc tttcagccgg cttctatcgg gggtgagttt taatgtatag
     3061 gcttcgccgg ccgtacgcaa tactttttct ccaatcactt tccccgcccg gtaagcaact
     3121 gctttcagtt cgccggcttc gtaaaccaca tcgtgccaca tcaaacgata t
//
LOCUS       DAOSGL010000271         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28771_length_3167_cov_4.355398, whole genome shotgun sequence.
ACCESSION   DAOSGL010000271 DAOSGL010000000
VERSION     DAOSGL010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28771_length_3167_cov_4.355398"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /gene="rbsC"
                     /locus_tag="PKY68_07935"
     CDS             complement(<1..952)
                     /gene="rbsC"
                     /locus_tag="PKY68_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235578.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HOH24998.1"
                     /translation="MKKNFKVIIDKPVIKPLIALLLMVGVMSVLSGNFATTDNFFNVL
                     RQVSVNLCISVGMTMVILTGGIDLSVGSILALSGAVAAGLSKNGFSFPAADLYVGFTF
                     WGCILAALLIGAALGAFNGWMITRFKVPPFVATLAMLTIARGLTMLYTKGFPITGLGE
                     SFSFLGTGWFLGVPMPVWVALLIVAFFAFFMFKTRTGRYIYAIGGNKKASLLSGIKVN
                     KIILWVYTLAGILSAVAGLMVTARLDSAQPNAGSGYELDSIAAVVIGGTSLSGGKGSI
                     AGTVVGALIIGVLNNGLVLLNVSPFWQQVIKGFVILGAVIL"
     gene            complement(983..2512)
                     /gene="gguA"
                     /locus_tag="PKY68_07940"
     CDS             complement(983..2512)
                     /gene="gguA"
                     /locus_tag="PKY68_07940"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOH24999.1"
                     /translation="MYKSDSEILLEARHISKQFPGVLALDDVGFKVYAGKVNAIVGEN
                     GAGKSTLMNILSGVYSDYQGEVLLCGRPVSFSGTADAQRQGIAMIHQELNLVPYLSIA
                     QNIFLGREPLNALGLIDFPKMKRDTVDLLERLHFDESPEKLVIDLRPGQQQIVEIAKA
                     LSLHAKVLIMDEPTSSLSEKEAGILFRLIRELTENGVGVVYITHKMDEIRLLADYVTV
                     LRDGCFIEACPIQQTSVDELVRKMVGRERKDFFIKKQHRPGECMLAVKDLCLKDPLRE
                     GKYRLKDISFEVYAGEVLGIYGLMGAGRTDLMEALFGLYGKQLEGRVFVKGKQVKFND
                     TNAAVAEGLALIPEERKTGGLVLDMSIEQNTTLASLKNFLKHGLLSFKKERATAEEMR
                     SKLEIKSYSGLQLASQLSGGNQQKVVLSKWLLTKPGIVLLDEPTRGIDIHSKNEIYKL
                     MDDLAGQGMALVVVSSELPEIMAVSDKIICLCEGRLSGRFERDEFSEEALLKAALPDK
                     A"
     gene            complement(2522..3145)
                     /locus_tag="PKY68_07945"
     CDS             complement(2522..3145)
                     /locus_tag="PKY68_07945"
                     /inference="COORDINATES: protein motif:HMM:NF021565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 family protein"
                     /protein_id="HOH25000.1"
                     /translation="MRKSLKIILALVAFILALYFSFYTESLSERQLKEQFKEFNPAQV
                     VDYCWEHKLEDLVRHAIPCEEFFAGLQGDAKSYAERQGKVLGIGSNYFFVMQAQAEIS
                     SVDEHNLYLILNRDVKATIPLVNIFSNLARDASAWFNPDDFQNTMDFNALSSYLNKKI
                     LQEVLAPLSERFSPGVRIEFVAAVEVNITDLPLKELVLIPYKIEILS"
BASE COUNT          870 a          832 c          660 g          805 t
ORIGIN      
        1 cgaggataac ggctccaaga atgacaaaac cctttatgac ttgctgccag aaaggagaaa
       61 cgttcaacaa aaccaaacca ttgttgagca ccccgataat caacgcgccc accaccgtac
      121 cggcaatgga acctttgccc ccactcaggg aagtgcctcc aataacaacg gcggctatgg
      181 aatcgagttc ataaccgctg cccgcgttgg gctgggcaga atccaatctg gctgtaacca
      241 tcagcccggc cacggcagac aatattcccg ccagggtgta cacccagagg atgattttat
      301 tcaccttgat gcccgacaac aaactggctt ttttgttgcc tccgatggcg taaatataac
      361 gtcctgtgcg ggttttgaac ataaaaaaag caaagaaggc cacgatcaac agggccaccc
      421 acaccggcat aggcactccc aaaaaccagc cggtacccaa aaaggaaaaa ctctcgccca
      481 gacccgtaat ggggaaaccc ttggtgtaca acatggtcaa tcccctggct atggttagca
      541 tggccaaggt agccacaaaa ggcggcacct tgaagcgggt gatcatccag ccgttgaagg
      601 ctcccagcgc agcaccgata agtaaagcgg ccagtataca gccccaaaag gtaaagccca
      661 cgtaaaggtc tgctgcggga aagctgaaac cgtttttact cagtccggcc gccacagcac
      721 cgctcagcgc caaaatagaa cccacggaaa gatcgatgcc tccggtcaga ataaccatgg
      781 tcattcccac cgatatgcaa aggttaacgg aaacctggcg caaaacattg aaaaaattgt
      841 cggtagtagc aaaatttccc gaaagcacgg acattactcc caccatcagc agtagagcaa
      901 tcagaggttt aattaccggt ttgtctataa tgactttaaa gttctttttc ataatcactg
      961 ctatttaggc cttatcgggt atttaagcct tatcgggtaa ggctgctttc aataaggctt
     1021 cttcagaaaa ctcgtcccgt tcaaaacgtc cgcttaacct cccctcgcag aggcaaatta
     1081 ttttgtcgga cacagccata atctccggta attcagaaga taccaccacc aaagccatgc
     1141 cctggccggc caaatcgtcc atcaatttat aaatttcatt tttggagtga atgtcgatgc
     1201 cccgggtagg ttcgtccaat aatacaatgc cgggcttagt cagcaaccac ttggacagaa
     1261 ccactttttg ctgattgcct ccgctgagtt gagaagccaa ttgtaaaccg gaatacgatt
     1321 taatttccag tttgcttctc atttcttctg ctgttgcccg ttctttttta aatgaaagca
     1381 ggccatgttt caaaaaattt ttaagagaag ccagagttgt attttgctcg atcgacatgt
     1441 cgagaaccag tcctccggtt tttctttctt cagggataag cgccaacccc tctgccactg
     1501 cggcatttgt atcgttaaat tttacttgct tgcctttgac aaacacccgg ccttccaatt
     1561 gcttgccgta caaaccaaac aaggcttcca tcaagtcggt ccgtccggca cccatcaagc
     1621 cgtaaatacc caatacctcg ccggcataca cttcaaaact tatgtctttc aggcggtatt
     1681 tgccttcgcg caatgggtcc ttgaggcaaa gatccttgac cgcaagcatg cattcccccg
     1741 ggcgatgttg tttttttata aaaaaatcct tgcgttccct tccgaccatc ttgcgcacca
     1801 attcatccac gctggtttgt tgtatcgggc aagcttcgat aaaacaaccg tctctcaaca
     1861 cagttacata atcagccagc agtcttatct cgtccatttt atgagtgata tacactactc
     1921 ccactccatt ttcggtgagt tcccttatca ggcgaaacaa tattccggcc tccttttcgg
     1981 acaaggaaga agtgggttcg tccataatca aaactttggc gtgaagagac agagcttttg
     2041 caatttccac tatttgctgc tgaccgggtc tgaggtctat gaccaacttt tcgggacttt
     2101 cgtcgaaatg caatctttcg agcaaatcga ccgtatccct tttcattttg gggaaatcta
     2161 tcaatcccaa ggcattcaat ggttccctgc ctaaaaaaat attctgggca atactcaaat
     2221 aaggaaccag attcaattcc tgatgtatca tggctattcc ctgcctctgg gcatcggccg
     2281 ttcccgaaaa gcttaccggc cggccgcaaa gcaagacttc tccctgatag tcggaataca
     2341 cccccgacaa aatattcatc agagttgatt tgcctgcccc gttttcaccg acaatggcat
     2401 ttactttacc tgcataaacc ttaaaaccga catcgtccag ggccaacacg ccgggaaact
     2461 gtttgctgat gtgcctggct tcgagcagaa tttcagaatc ggacttatac atggaactgc
     2521 cttaactcag tatttcgatt ttatagggaa tcagcaccag ttctttcaag ggcaaatcag
     2581 tgatatttac ctcgacggca gcaacgaact ctattctgac tccggggctg aaccgttcgc
     2641 tcagaggagc cagtacctcc tgaagaattt ttttgttcag gtacgacgac agagcattaa
     2701 aatccatcgt gttttgaaaa tcgtcggggt tgaaccaggc ggaggcatcg cgagccaggt
     2761 tactgaatat atttaccaag ggaatggtcg cttttacatc ccggttcagg atcaggtata
     2821 gattatgttc atcgacagag gaaatttcgg cttgagcctg cattacaaag aaataattgg
     2881 agcctatgcc cagcacttta ccctgccttt cggcatagga tttggcgtcg ccttgcaaac
     2941 ctgcgaaaaa ctcttcgcaa ggaatagcgt gccgaaccaa atcctccagt ttgtgttccc
     3001 aacagtaatc gaccacctga gcgggattga attctttgaa ctgttctttt aattgcctct
     3061 cactcagcga ctcggtataa aaagaaaaat acagagcaag gataaaagcc accagggcaa
     3121 ggatgatttt taaacttttt cgcatcgctt attccagttt ctcaaat
//
LOCUS       DAOSGL010000272         3163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_28819_length_3163_cov_3.130952, whole genome shotgun sequence.
ACCESSION   DAOSGL010000272 DAOSGL010000000
VERSION     DAOSGL010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28819_length_3163_cov_3.130952"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="PKY68_07950"
     CDS             complement(<1..1358)
                     /locus_tag="PKY68_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH25001.1"
                     /translation="MREIEQLIEISRFYGKDKRFVIAGGGNTSYKDERQIWVKASGFA
                     LATIDRDGFAVLDRDKLRLLSEKSYSDDTDLREAEVKSDLEAACITKNRRPSVETSMH
                     NAINYAFVVHLHPTAVNGLMCSRQAENKLRELFGDEHLYIPYTDPGYVLFKKVEGAIA
                     AFRAKRGKEPSVIWLQNHGIFVAADNIEEIRRTYDHILAVLDKEVKTAAHGAAAGSKA
                     LQAYLQPEERPVPGRVIEILPALRMLVSETDEQKRLQLKTLKIRNNKLIEYYCEKKEH
                     YIYISRPFTPDGIVYCKSNQLYIDKAKTEDILDDAKKILPFFRQHFGYQPKVIALRGL
                     GIVAVGDNAAQCDTILDVFEDCIKIAAVSEAFGGPHPMTQEQIDFIDHWEVENYRRSI
                     AAGALTGRAQNKTIVVTGAAQGFGQGIADELLKEGANIVVADLNSETGLVTVDEFNQK
                     AR"
     gene            complement(1422..2633)
                     /locus_tag="PKY68_07955"
     CDS             complement(1422..2633)
                     /locus_tag="PKY68_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25002.1"
                     /translation="MKIVNVNAHLHTPYSFSAFESLTQALDLALEQGVRVVGINDFYS
                     MDGYELWEKECRKRSLYPLFNIEFIGLNQQDQAQGIRVNDPNNPGRTYISGKGLKCPP
                     SLPEPYASQLSLVRAESNAQVKAMCAKINDLLDSLRLGFRLDYEQILREMTKGNIRER
                     HLAKALRIKVYEQALGSASGSKKQVSEAGREAEAKIAALFQQLFGGKALKSAINDHAA
                     VENEIRGNLLKAGGAAFVPEDPKAFLPVDKVCSIILAAGGIPTYPFLADDPKGGYTDF
                     EGDLEKAAKTLHERGFHSVEFISTRNDLHLLEKYVVYLYELGFVVTLGSEHNSPAMEP
                     ILLSARHGQALSEVLREINYKSACVLAAHQHLVGQGLEGYVDASGRAHTDKREEYIKL
                     GDQLIRSVTGQ"
     gene            complement(2727..>3163)
                     /locus_tag="PKY68_07960"
     CDS             complement(2727..>3163)
                     /locus_tag="PKY68_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOH25003.1"
                     /translation="TILTIGATLYRALEAADILQEQYGISAEVIDARSIVPFNYDLVI
                     ESVKKTGRILLSSDASSRGSIINDMARNISELAFDYLDAPPVVVGSRNWITPAHELED
                     AFFPQAGWMLDAIHEKIQPLKNYIPKQNFSNSQQILRNKQGV"
BASE COUNT          718 a          748 c          833 g          864 t
ORIGIN      
        1 ctgcgggctt tttggttgaa ttcatccacg gtgaccagac cggtttcgct gttgaggtcg
       61 gccaccacaa tattggcccc ttccttcagg agttcatcgg caataccctg gccaaagccc
      121 tgggcagctc cggtaaccac tatagttttg ttttgggctc tgccggtcag ggcgccggcg
      181 gcgatgctgc ggcgatagtt ttcgacttcc cagtgatcga taaaatcgat ttgttcctga
      241 gtcatgggat gcgggcctcc aaaggcttcg gagacggcgg caatcttgat gcagtcctca
      301 aaaacatcga ggatggtatc gcattgggct gcgttgtcgc ctacggccac gatacccaaa
      361 ccccggaggg caatcacctt gggttgatag ccgaaatgtt gcctgaaaaa aggcaggatt
      421 tttttcgcat catccagtat atcttcggtt ttggccttat ctatatataa ctggttggat
      481 ttgcaataca ctatgccgtc gggcgtgaag ggacggctga tgtatatata atgctctttt
      541 ttctcacaat agtactctat cagcttgttg ttgcggattt tgagtgtttt tagctgaaga
      601 cgtttttgct catctgtttc ggaaaccaac atacggagcg caggcagaat ttcgatcact
      661 ctgcccggaa caggacgttc ttccggctga agataggcct gcagggcctt ggagccggca
      721 gcagccccat gagcggccgt ttttacttct ttgtccaaca cagccaggat atgatcgtag
      781 gtacggcgaa tttcttcgat gttgtcggct gccacaaaga taccgtggtt ctgcaaccag
      841 atcaccgagg gttctttgcc ccgctttgcc cggaaggcag caatggctcc ttcgactttt
      901 ttgaatagga catagccggg atcggtataa ggaatgtaga gatgttcatc gccaaacagt
      961 tccctaagct tattttcggc ttgccgggaa cacatcagcc cgttgacggc cgtgggatgc
     1021 aaatgcacca caaaagcgta gttgatggca ttgtgcatgg aagtttctac cgacgggcgc
     1081 cggtttttgg taatacaggc agcttcgagg tcgcttttga cttcggcctc gcgcaggtcg
     1141 gtatcgtcgc tgtaggactt ttcggagagg agccgcagtt tgtcgcgatc gagtacggca
     1201 aaaccatccc gatcgatggt ggccagagca aagccggagg ctttcaccca gatttgccgc
     1261 tcgtctttat aagaggtgtt gcctccgcct gcaataacaa aacgcttgtc tttaccataa
     1321 aaccgggata tctcgatgag ttgttcgatc tctctcatga cattattatt taattgatta
     1381 gaaaatgaaa agtccatact tgtgttctta tttattcttg tttattggcc ggtgacggaa
     1441 cggatgagct ggtcgcccag cttgatgtat tcttcgcgct tatctgtgtg agcacggcct
     1501 gaagcatcca cgtagccttc gaggccctga ccgaccaggt gttgatgggc tgccagcacg
     1561 caggcgcttt tgtaattgat ttcacgcaaa acttcgctca atgcttgtcc gtgacgggca
     1621 gacaacaaaa tgggctccat ggcggggctg ttgtgctcgc tgcccaaagt gacgacaaaa
     1681 ccgagttcgt agagataaac gacgtatttt tccaatagat gcaggtcgtt gcgggtgctg
     1741 atgaattcca ccgaatgaaa accccgttca tgcagggtct tggctgcttt ttccagatcg
     1801 ccttcgaagt cggtatagcc tcctttggga tcgtcggcca aaaaaggata agtgggtatg
     1861 ccgccggcag ccaatattat gctacagact ttgtccacag gcaaaaaggc tttgggatct
     1921 tcgggtacaa aagcggcacc gccggccttg agcaaattgc cccggatttc gttttcgacg
     1981 gcagcatggt cgtttatggc agacttgagg gctttgcctc cgaacagttg ctgaaaaagg
     2041 gctgcaattt ttgcttcggc ttcccggccg gcctccgaaa cctgcttttt gcttccggaa
     2101 gcggaaccta aggcttgttc gtagacctta atgcgcaaag ccttggccaa atgccgttcg
     2161 cggatgttgc ccttggtcat ttctctgagg atctgctcgt aatcgaggcg gaaacccagc
     2221 ctcagactat cgagcaagtc gttgattttg gcgcacatgg ccttgacctg ggcattggat
     2281 tcggctctta ctaggctgag ttgagatgcg taaggttcgg gcaacgaggg cggacattta
     2341 agacccttgc cactgatata ggtacggccg ggattgttgg gatcgtttac ccgtatgcct
     2401 tgggcctgat cctgctggtt gaggccgata aattctatat tgaacaaagg ataaagcgag
     2461 cgcttgcggc attctttttc ccagagttcg tagccgtcca tggaataaaa gtcgttgatt
     2521 ccgaccaccc tgaccccctg ctccaacgcc aggtcgagag cctgtgtaag gctctcgaag
     2581 gcgctgaacg aataaggcgt atgcaaatga gcattaacgt tcactatttt cattgttttt
     2641 atttttattc agtgattccg tattggataa atggtttttt aattatcaat gaaatattaa
     2701 aaaaactgat tcaaacggca aatatactaa actccctgtt tattcctaag aatctgttga
     2761 gaatttgaaa aattttgttt gggaatgtaa tttttcaagg gttgaatttt ctcatgaatg
     2821 gcgtccagca tccaacctgc ctgggggaag aaagcatctt ccaattcatg ggcgggtgtg
     2881 atccagttgc gtgaacccac taccacggga ggagcgtcca ggtaatcgaa agccagttcg
     2941 cttatgttgc gggccatatc gttgataatg gatccgcggc tactggcgtc gctcgaaagg
     3001 agaatacgtc cggtcttttt gaccgactca attaccagat cgtaattgaa gggcactatg
     3061 ctgcgggcat cgatcacttc ggcgctgata ccgtattgtt cctgcaatat gtcggcagct
     3121 tcgagagcac ggtaaagggt agctcctatg gtgagaatgg tta
//
LOCUS       DAOSGL010000273         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_29069_length_3144_cov_4.372612, whole genome shotgun sequence.
ACCESSION   DAOSGL010000273 DAOSGL010000000
VERSION     DAOSGL010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29069_length_3144_cov_4.372612"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKY68_07965"
     CDS             complement(<1..818)
                     /locus_tag="PKY68_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH25004.1"
                     /translation="MKNPLSALPALLIIATGLYACSGASKSREAQLETTQEKIENVKV
                     TTLSSRMVGNTLEFPANTRAYERVNLGPVSPGRIEKIYVEMGDRVKKGQLLIQMDQTA
                     LLQARIQIANFEEDHRRYEALKESGAISQQVYDQSLANLNMQKTNLDYLEKNTRIKAP
                     FDGIIALRNFENGELYPGGSAILTLMEIDKLKADINVPESYYPLIKKGMKAEVFCDTY
                     PGRPFEAEVNLIAPTVNPASRSFAVEMKIPNAQEWLKPGMFVRIHMDLGVSEAI"
     gene            complement(941..2263)
                     /locus_tag="PKY68_07970"
     CDS             complement(941..2263)
                     /locus_tag="PKY68_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH25005.1"
                     /translation="MKTIIPSNRVKLFTLCLSGLLSLSSLPFTSQAEETPQQWRFSLE
                     EAVGHALVHNTAMIKADLAVRQAEAAKWAAIANYLPQLNVNVNYNNYLGASIEIFGMD
                     IPMDPSSRLTLQASQLIFNANAILGMQLSELSRRMSELGSNQSELAVKQNVRSTYYSI
                     LMMENNRLLLKKNVENMRSLAKATRAKVEVGIGEQTEADEIEIVLANLENTLKQTERT
                     IELTYNSMRLLLGLNAEDGIELSDRLQDVTDKQAMFALLAAGFDPAENVDMKAGQLNL
                     EINKKQVKSAIAGYLPTISAVYQRNEILLKTGFDPTTKNTLVLTASMPLFCSGKTTAE
                     LKKAQLAYESAQEDLELMQKQLLIQDKQLRFNLKSALESYEIQERNIEVSKRMFDNVT
                     KKFEQGLSSSLEVTNASNNLLTAQSNYIQSAMSLLNASDALKKLLGTL"
     gene            complement(2350..2988)
                     /locus_tag="PKY68_07975"
     CDS             complement(2350..2988)
                     /locus_tag="PKY68_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH25006.1"
                     /translation="MADLLREDILKTSTRLFFKFGLRSVSIEDICNELRISKKNFYTC
                     FSQKEALIEDILIDYDEAFAKRMELKIKSLNLPESTTVIDMVLFFSVFHLINRDARFE
                     NFFFDMQKYYPEVFKRHMDRHRHIVTNHIRDMLLKGIEQGLYRAELETELTAKIITLE
                     LISMMEFARNKLNKSEQQKVIMLLTDLHMRSLCNERGLKYYLKALDKLENQQ"
BASE COUNT          707 a          691 c          747 g          999 t
ORIGIN      
        1 aagatggctt cgctcactcc cagatccata tggatgcgaa caaacatacc tggcttgagc
       61 cattcttggg cgttgggaat cttcatttca acggcaaaag aacggctggc cggattgacc
      121 gtaggtgcaa tcaaattgac ttcggcttcg aatgggcgac cggggtaggt atcgcaaaat
      181 acttcggcct tcatcccttt tttgatcagt ggataatagc tttcgggtac gttgatgtct
      241 gctttgagtt tatcgatttc catcagtgtc agtatggccg aaccgcccgg gtaaagttcg
      301 ccgttttcga aattacgcag ggcgatgatc ccgtcgaagg gcgctttaat ccgggtgttt
      361 ttttccaggt agtccaggtt ggttttttgc atgttcaggt tggccaacga ctggtcgtaa
      421 acctgttgcg aaatagctcc gctctctttg agcgcttcat agcgcctatg gtcttcctca
      481 aaattggcga tctgaatgcg tgcctgaagc aacgcggtct gatccatttg aataagcaat
      541 tgtccttttt tgacacggtc gcccatctca acgtaaattt tttcaatacg gcccggcgat
      601 accggcccca gattgacccg ttcgtaagcc ctggtattag ccgggaattc cagggtattg
      661 cccaccatac gcgaactcag ggttgttact ttgacatttt cgatcttttc ctgagttgtt
      721 tctagttgtg cctcccgcga tttggaagca ccggagcagg catacagccc cgttgcgata
      781 attagcaggg ccggaagtgc agaaagcgga tttttcattg cagatgtttt ttattgtttt
      841 atttcctgtt tttatttcct gttgttgttg atgatgtttt tgttgttgct gttgttactg
      901 ttgttgttgc tgctactgat gataatgatg attgaaccgt ttataatgtc cccagcaatt
      961 ttttcaaagc gtcgcttgcg ttgagcaagc tcatggccga ctggatgtaa ttgctttgtg
     1021 cggtaagcag gttgttgctg gcgttggtca cttcgaggct ggacgataag ccttgctcga
     1081 atttcttggt cacgttgtcg aacatgcgct tcgaaacctc tatgttgcgt tcttgtattt
     1141 cgtaagattc cagagcagac ttcaagttaa aacgcaactg tttgtcctga atcaataact
     1201 gtttttgcat aagttccaga tcttcctgcg ccgattcgta ggctagttgg gcttttttta
     1261 gttcggcggt ggttttgccg ctgcagaaca gaggcatgct ggccgtgaga accagcgtat
     1321 ttttggtggt cggatcaaaa cccgttttca gcaaaatctc gttgcgctga tatacggccg
     1381 aaatggtcgg taaataaccg gcaatggccg actttacttg ttttttgttg atttccaggt
     1441 tgagctgtcc ggccttcata tcgacgtttt cggccggatc aaaaccggct gccagcaagg
     1501 caaacatggc ctgtttgtcg gtaacgtcct gcagtctgtc gctcagttct atcccatcct
     1561 cggcgttcag ccccaagagt agccgcatgg aattataggt caattctatg gttctttcgg
     1621 tttgtttcag ggtgttctcc agattggcca gcactatttc aatttcgtcg gcttcggtct
     1681 gttcgcctat gcccacctcc actttagctc tcgtagcctt ggccagactg cgcatgtttt
     1741 ccacgttttt tttgagcaaa agccggttgt tttccatcat caaaatggag tagtaggtgg
     1801 acctcacgtt ttgtttaact gccagttccg attgattgct tcccagctcc gacattcggc
     1861 ggcttagttc gctcaattgc atacccagga tggcattagc gttaaaaatc aattgcgaag
     1921 cctgcagcgt cagcctgctc gaggggtcca tgggaatgtc cattccgaaa atttcgatag
     1981 aagcgcccag gtagttattg taattaacgt ttacattaag ctgaggcagg tagttggcta
     2041 tagccgccca tttggcggct tcagcctgac gcactgccag gtcggctttg atcatggctg
     2101 tgttgtgtac caaggcatga ccgacggcct cttcgagcga aaaacgccat tgttggggtg
     2161 tttcttccgc ctggcttgtg aacggcaggc tgctcaggct taacagcccc gacaggcaga
     2221 gcgtgaacag ttttactcta tttgatggaa taatggtttt cattgctatt taattctata
     2281 tctgtttttg ttttttgatt cttggttttt aaaatccctg tcgattggtt acggctttgt
     2341 accaccgatt cactgttgat tttcgagttt atcgagggcc ttgaggtaat acttaaggcc
     2401 acgctcgttg cataaacttc tcatatgcag gtcggtgagc aacattatca ctttttgttg
     2461 ttcgctttta ttcagtttgt ttctggcgaa ttccatcatg gaaatcaact cgagagtaat
     2521 tattttggcg gtaagctccg tctctaattc ggcacgatag agcccctgtt caattccttt
     2581 gagcagcata tcgcggatgt gatttgtcac tatatgccgg tgacgatcca tatggcgttt
     2641 aaatacctca ggataatatt tttgcatatc aaaaaagaag ttttcgaacc gggcgtcgcg
     2701 gtttatcaga tgaaaaaccg aaaagaacag caccatgtct atcactgtgg tcgattccgg
     2761 cagattgagg cttttgattt tcaattccat acgtttggcg aaagcctcgt cgtaatcgat
     2821 tagtatatct tcgatcagag cctctttttg cgaaaagcaa gtgtagaagt ttttttttga
     2881 aatgcgcagt tcgttgcaga tatcctcgat ggatacactg cgcaggccga atttaaaaaa
     2941 cagcctggtc gaggttttaa gtatatcttc gcgaagtaaa tctgccatta tgaagtaatt
     3001 ttttgccgtc gtgaagtaag tttgttatta acctaaaaaa cgcggcaaag gtaaagtcaa
     3061 aaccgatttt ccgatatccc ggtgcgttaa atatgtttaa acaacaaaca atattttaca
     3121 acaatctgat aaatagatac ataa
//
LOCUS       DAOSGL010000274         3128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_29284_length_3128_cov_4.498861, whole genome shotgun sequence.
ACCESSION   DAOSGL010000274 DAOSGL010000000
VERSION     DAOSGL010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29284_length_3128_cov_4.498861"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKY68_07980"
     CDS             complement(<1..795)
                     /locus_tag="PKY68_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOH25007.1"
                     /translation="MKNNFLAILLAAVVFFALCLGLSSCRGGKKKVKGKDKIAVVIST
                     LNNPWFVVLGETAKEKAIELGYDAVLFDSQNNPSKETEHFDNILSSGYKAVLFNPTDA
                     NGSVVNVKAAKKAGIPVFCMDREINDSEAAVSQILSDNFSGCVALGQYFVKKFDGKGN
                     YVEILGLVGDNNTWNRSKGFHSVVDNFPQLVMLAQQTADFDQNKAMEVMESMMQAHSN
                     IDAVFCGNDAMAMGAYQAILAAGKAGRILVFGFDGSQDAINAIKEGK"
     gene            complement(849..2267)
                     /locus_tag="PKY68_07985"
     CDS             complement(849..2267)
                     /locus_tag="PKY68_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOH25008.1"
                     /translation="MSYKKQLCFGVIIGTRNYFNSALAKDVRKSLLESLTKAGHTYVI
                     LDESQTATGSIETREDGIKCAKLFREHRDCIDGILISLPNFGFEIGIINALDLAELKV
                     PILVQACDDDNDKVGLDSRRDAYCGKISVCNNLYQYNIPFSLTSLHTCAIDSKEFAAD
                     LDYFAGICRVVRGLHKARIGAIGTRPAGFQTVRASEKILQASGITVVPVDLSEIMAAA
                     NKYDNNSAELKQRVEEIKAYARVPEKFAEKLKVQAKFGLAVEAWIKENEVDATAIQCW
                     DSLEKNYGCAACVSMSMMSEKLIPSACETDIAGAISMYMLTLASGQPAALADWNNNFA
                     REHDKCVCTHCGNFPKSFVKNPLELGTLGVLGSVLGSEATFGAVKGKVSAGDFSFFRI
                     STDDNFGLIKAYLGEGEITDEPYGMDGCIAVTKVNNLQQLMRFICKNGFEHHVAMCRS
                     RVADVLEEAIDTYLGWDLYRHR"
     gene            complement(2279..>3128)
                     /locus_tag="PKY68_07990"
     CDS             complement(2279..>3128)
                     /locus_tag="PKY68_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOH25009.1"
                     /translation="MLPRVLACDQVFGNTDFRGLLPRAVPIAGVLGDSHGALVGQSCF
                     TPGMGKATYGTGSSVMVNIGSTPLKAPEGLVTSVAFAALGKTYYAFEGNIHCTGATIK
                     WMEDELGLLTGSAESEALALSVDDNAGVYLVPAFAGLGAPWWKPDARAIICGLTRGSN
                     RAHLVRAGLEAIAYQVKDLVDLMLSQAGTTLEELRVDGGPVRNRFLMQFQADMLNAPI
                     NVSPIEEASALGAVYMNFLAQGRVRSLEQLSQLKIRQEQLKPRMNAAQREQLYAGWQQ
                     AVKMIL"
BASE COUNT          801 a          816 c          702 g          809 t
ORIGIN      
        1 tttaccttct ttgatggcgt taatggcatc ctgacttccg tcaaaaccaa agaccagaat
       61 acggccggcc ttgcctgctg ccaggatggc ctgataggct cccatagcca tagcatcgtt
      121 gccgcaaaag accgcatcaa tattggaatg cgcctgcatc atcgactcca ttacctccat
      181 ggctttgttt tgatcaaaat cggctgtttg ttgagccagc atgaccaatt gaggaaaatt
      241 atccactaca ctgtggaaac ccttagaacg gttccaggta ttgttatcgc ccacaagacc
      301 cagtatctct acgtaattgc ctttgccgtc gaatttcttt acaaaatact gtcccagggc
      361 cacgcaaccc gaaaagttat cggaaagaat ctgagaaacg gcagcttctg agtcgttgat
      421 ttccctgtcc atacaaaaca ccgggatgcc ggcctttttg gcagctttta cgttgaccac
      481 cgaaccgttg gcatcggtag gattaaataa aacagcctta taacccgagg acaaaatatt
      541 gtcaaaatgt tccgtttctt tcgaaggatt gttttgagaa tcaaacaata cggcatcgta
      601 ccccaattca atggcttttt ctttggccgt ctcacccaga acgacaaacc aaggattatt
      661 gagcgttgaa atcacaacag ctattttgtc tttacccttc accttctttt tacctccccg
      721 gcaagagctt aagcctaagc ataaagcaaa gaaaacgaca gccgctagta gtatggctaa
      781 gaaattgttt ttcatcgtat tgtttattta aaatctacaa atactactct catcgccccg
      841 gggaaatttt aacggtggcg gtacaaatcc cagcccaaat aagtatcaat agcttcttcg
      901 aggacatcag ccacccggct gcgacacata gccacgtgat gttcaaaacc gtttttgcat
      961 ataaacctca tcaactgctg aagattattt acttttgtaa cggcaatgca accatccata
     1021 ccatagggct cgtcggtaat ttcgccttca cccagataag ccttgatcag gccgaaattg
     1081 tcgtcggtcg aaatcctgaa aaaggagaaa tcacctgcag aaactttgcc tttgacggcg
     1141 ccaaaagtgg cttcactgcc caacacacta cccaatacac ccaatgttcc cagttctaac
     1201 gggttcttga caaagctttt ggggaaatta ccgcaatgcg tgcaaacaca tttatcgtgt
     1261 tcacgggcaa aattattgtt ccagtcggcc aaagctgccg gttgacccga agccaaagtg
     1321 agcatataca tggaaatggc ccccgcaata tccgtctcgc aggcgctggg tatgagtttt
     1381 tcgctcatca tactcatgct gacgcaagct gcgcaaccat agtttttttc gagcgaatcc
     1441 cagcactgaa tagccgtagc gtccacttcg ttttctttga tccaggcctc aacggccaat
     1501 ccgaatttag cctgaacttt cagtttttcg gcaaatttct caggaacccg ggcataagcc
     1561 ttgatttctt cgacacgttg tttaagctca gccgagttgt tgtcgtactt atttgcagcc
     1621 gccattatct cggacaaatc caccggaacc accgtgatgc cgctagcttg caagattttt
     1681 tcgctggccc tgacggtttg gaaaccggcc ggacgagtac cgatggcacc gatgcgggcc
     1741 ttgtgtaaac cacgaaccac gcgacaaatc ccggcaaaat aatccaaatc ggcggcaaac
     1801 tctttgctgt ctatggcaca ggtatgcaga gaggtcaggc tgaaaggaat attgtattga
     1861 taaaggttgt tgcaaaccga tatttttccg caataggcat cccgtctact gtccaggcct
     1921 accttgtcgt tatcgtcgtc gcaggcctgt accaaaatgg gaaccttgag ttcagccaga
     1981 tccaaggcgt taataatacc tatttcgaaa ccgaaattgg gcagggaaat caatattcca
     2041 tcgatgcagt cgcggtgttc cctaaaaagt ttggcgcatt tgatgccgtc ttcccgggtt
     2101 tcgatactgc cggtggctgt ttgactttca tcgagaataa cgtaggtgtg accggctttg
     2161 gtaaggcttt ccaacaaaga ttttctcacg tctttggcca gggccgagtt gaaataatta
     2221 cgggtgccta ttatgacccc gaaacaaagt tgctttttgt atgacatagt tgtaaggttt
     2281 ataaaatcat ttttacggct tgttgccagc cggcgtataa ttgttctctt tgtgcagcat
     2341 tcatccgagg cttgagttgt tcctgacgga ttttgagttg actcagttgc tccaggctcc
     2401 ggacccggcc ctgggccaga aaattcatat acacggcgcc cagtgcagaa gcttcttcaa
     2461 tgggacttac gttgatgggg gcatttagca tgtcggcctg aaactgcatc aggaaacggt
     2521 ttcgtaccgg acctccgtca acccggagct cttcgagtgt tgtcccggcc tgactgagca
     2581 tcagatctac cagatctttt acctgatagg ctatggcctc aaggccggca cgtactaggt
     2641 gtgctctgtt gctgccacgg gtcaaaccgc atataatagc tctggcatcg ggtttccacc
     2701 agggcgcacc caaaccggca aatgccggaa caagataaac tccggcgttg tcgtcaacgc
     2761 tcaaagccag ggcctcgctt tcggcagagc cggtcaacaa gccgagctcg tcctccatcc
     2821 atttaatggt tgctccggtg caatgaatat tgccttcgaa agcgtaatat gtctttccca
     2881 gggcggcaaa agccaccgaa gttaccaaac cttcgggagc cttcagagga gtcgaaccta
     2941 tgttgaccat caccgaggaa cccgttccgt aagtagcttt acccattccc ggggtaaagc
     3001 agctttgtcc gaccagggct ccgtgagaat cgcccagtac tccggctata ggtacagccc
     3061 ggggtaacag accacgaaag tctgtattgc caaacacttg atcgcaagcc aatacccggg
     3121 gcagcatg
//
LOCUS       DAOSGL010000275         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_29577_length_3107_cov_3.931193, whole genome shotgun sequence.
ACCESSION   DAOSGL010000275 DAOSGL010000000
VERSION     DAOSGL010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29577_length_3107_cov_3.931193"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2153)
                     /locus_tag="PKY68_07995"
     CDS             complement(<1..2153)
                     /locus_tag="PKY68_07995"
                     /inference="COORDINATES: protein motif:HMM:NF027309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HOH25010.1"
                     /translation="MRTPNLYCIKTGLPLILLCFAVFCGSCRQGSTGGSRTELLFERE
                     KTTTLWLDTGEAEVVYTAVDLFRRDVQLVFNADLKIVEDKKDADIVLAFSGLKRPASS
                     GPKKFSPSWLKRSAVAGPKKLAFFKCHRSSFSKPNSRGGEAFRIRLCKSGRKPQLIVS
                     GSEPRGLAYGILELSRMIGVSPWHYFADSRPKELESFEFPLKRIEQQASVKYRGIFIN
                     DEDWGLMPWASQTLAPQQGKGVIGPEAYEKIFELLLRLRANTIWPAMHECTRPFYQVE
                     GNAQMARKYGILVGSSHCEPLARNSASEWDMDGSGDYNFMTNPSNVIQYWAGRLQELS
                     PGENIFTLGMRGKHDGLMQGVKTLEEHKAALSRIIPVQQDLLKQYIDADIEAIPQVFI
                     PYKEVMEVYDAGLEVPDHVTLVWCDDNYGYIRRLSNAREQERSGASGIYYHVSYWGRP
                     HDYLWLASTSPGLIYSEMLRAYKHGADRLWILNVGDIKPAEYLMEYFLDLAWDINAVD
                     NDFGHLQRFMHREFGAEQATALSEVYERYYWLATIRKPEFMGWSRVEESGYGRGGKTP
                     VRDAEYHPEFNKELQHRLEAYRELEQRVAEIRLLIPGQQLSCFFQLVEYPIRAASLMN
                     QKWLYARLARYYSDSCPELARLCAALSLQAYEGIEQLTATYNSLEEGKWERIMDFRPR
                     ELPVFDKPVFNNPELDGPEQKSSEFGKFMFEGPEF"
     gene            complement(2389..>3107)
                     /locus_tag="PKY68_08000"
     CDS             complement(2389..>3107)
                     /locus_tag="PKY68_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HOH25011.1"
                     /translation="PETNTEPVSGRPYLIASRCSGLMLQVEGDGVLINDYRPADSSQL
                     WLLQKAGEGYTIGNLQGLRLNDDGSYFLKTTSTFDPNNQLFDLDLRNDNFFKISSLST
                     GKALDLQGANYTAGTRVGLYGYDEGGADKVVTRQWMFLRPPRRLSDFNTSLFPPEHET
                     ALEVQLSVAPGMVQLFCPTFGHDGKTYRLRLFNLSGIMVRESRLNSAITRLSLPEGFY
                     LLSIDAGAEEKIFVQKIIVP"
BASE COUNT          778 a          830 c          725 g          774 t
ORIGIN      
        1 tcgaactccg ggccttcgaa catgaatttt ccaaactctg agcttttctg ttcaggacca
       61 tcgagttccg gattattaaa caccggtttg tcgaacaccg gcagttctcg gggcctgaaa
      121 tccattatcc tctcccattt gccctcttcc agcgaattgt atgttgccgt taactgctca
      181 ataccttcgt aagcctgcag gctcagggcg gcgcaaagcc gggcaagctc cggacaacta
      241 tcgctgtaat aacgagccag gcgggcatac agccatttct gattcatcag tgaggccgca
      301 cgaatggggt attctaccaa ttgaaaaaag caggacaatt gctgcccggg aataaggaga
      361 cgaatttcgg ccactcgctg ttccagttcg cgataagcct ccagtcgatg ttgtaattcc
      421 ttgttaaact ccggatggta ttccgcatcg cggacaggag tttttcctcc ccggccgtaa
      481 ccggattctt ctacccggct ccagcccata aactcgggct tacgaatggt agccaaccag
      541 taatagcgct catagacttc actcagcgct gttgcctgtt ctgcgccaaa ttccctatgc
      601 atgaaacgct gcaaatgccc aaaatcattg tcaaccgcat tgatatccca ggccaggtcc
      661 aggaaatatt ccatcaggta ttcggccggc ttgatgtcgc ccacattcaa tatccacaaa
      721 cgatcggccc cgtgcttata agcccgcagc atttcggaat aaatcaagcc gggcgaagtg
      781 cttgccagcc ataggtaatc gtgcggacgt ccccaatacg acacatgata ataaataccc
      841 gaagcgccgc tgcgttcttg ttcccgggcg ttcgacagcc ggcggatgta accgtagttg
      901 tcgtcgcacc acaccaaagt gacatgatcc ggcacctcca ggccggcgtc atagacctcc
      961 atcacttcct tgtaaggaat aaaaacctgg ggaatcgctt ctatatcggc atcaatatac
     1021 tgtttgagca agtcctgctg aaccggaata atccggctca gagccgcttt gtgctcttcg
     1081 agcgttttca ccccctgcat caggccgtcg tgcttacccc tcattccgag ggtaaaaata
     1141 ttctctccgg gactaagttc ctgcaaacgt ccggcccaat attggattac attcgaagga
     1201 ttagtcataa aattatagtc gccgcttccg tccatatccc attcggaggc ggaattgcga
     1261 gccaggggct cacagtggga acttcctacc agaatgccgt atttacgggc catttgggca
     1321 ttgccttcga cctgataaaa gggccgggta cattcgtgca tggccggcca aatggtattg
     1381 gcgcgcaaac gcaacaaaag ctcaaatatc ttttcgtaag cctccggacc gatcactcct
     1441 ttgccttgct gcggagccag agtttgcgaa gcccagggca tcagccccca atcttcgtca
     1501 ttgataaata tgccccggta tttcacggag gcttgctgtt ctattctttt cagagggaat
     1561 tcaaaagatt caagttcttt cgggcgggaa tcggcaaaat aatgccaggg cgacaccccg
     1621 atcatcctcg aaagttccaa tattccataa gccagccccc gtggctcact gccgctcacg
     1681 atcagttgag gtttccggcc gcttttgcac aaacggattc taaaggcttc ccctccccgg
     1741 ctgtttggtt tggagaacga agacctatgg cacttgaaga atgcgagttt ttttggcccg
     1801 gcaactgcag atcttttcag ccaagaaggc gaaaactttt tcggcccgga ggaagcaggc
     1861 cttttcagcc cggaaaaagc aagcacaata tccgcgtctt ttttatcctc aactatcttt
     1921 aaatccgcat tgaacacaag ctgaacatcc ctgcgaaaca aatccacagc cgtatacacc
     1981 acctctgcct cgccggtatc cagccaaaga gtagtggtct tttccctttc aaacagaagc
     2041 tccgttcggc tccctcctgt ggagccttgc cggcaactgc cacagaaaac ggcaaagcaa
     2101 agcagaatta aaggcaaacc ggttttaatg caatagagat tcggagttct cattctggtt
     2161 taatggtaat ggaggtttgg ggctttcatt tcggtttttg ctctagtgga gattcggggc
     2221 tttcattccg gtttttgctg tagtgaagat tcggggttct cattattatt actccgtttt
     2281 gttagggttc ctttttgggt gattttaggg ttttcaagct tcaaaggaga cgtgtttttt
     2341 aataaacaaa aacacactgc aattatgttt gccggggcct tgttttattt agggaacaat
     2401 gatcttttga acaaaaatct tttcctcggc tccggcatct atactcaaga gataaaatcc
     2461 ctccggcaat gacaaacggg taatagctga attcaaacgg ctttctcgaa ccatgatccc
     2521 cgacaagttg aagaggcgaa gacggtaggt ctttccatca tgtccaaaag tggggcaaaa
     2581 aagctgtacc attccgggag caacactcaa ctgaacttcc aaagccgtct catgctccgg
     2641 cgggaacaga gaagtattga agtcggagag tcggcgcggc ggacgtaaaa acatccattg
     2701 acgtgtaacg accttgtctg cacccccttc atcgtaaccg tacaagccca cccgggtacc
     2761 ggccgtgtaa ttagctccct gcaaatcgag agctttcccg gtagaaagcg aggagatttt
     2821 gaaaaaatta tcgttacgca agtccaggtc aaaaagctga ttattcggat caaaggtcga
     2881 agtagtcttt aaaaaataac tgccatcgtc gttgagccgg agtccctgca aattgccgat
     2941 agtataaccc tcgccggctt tttgcaaaag ccagagctga gaggaatcgg ccggccggta
     3001 atcgttgatc agcactccat cgccctcaac ctgcaacatc agacccgaac agcgcgatgc
     3061 gatcaggtag ggccggccgc ttaccggttc ggtattcgtt tccggag
//
LOCUS       DAOSGL010000276         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_29715_length_3096_cov_4.089444, whole genome shotgun sequence.
ACCESSION   DAOSGL010000276 DAOSGL010000000
VERSION     DAOSGL010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29715_length_3096_cov_4.089444"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2461)
                     /locus_tag="PKY68_08005"
     CDS             complement(<1..2461)
                     /locus_tag="PKY68_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH25012.1"
                     /translation="MVSGVVKDAAGEPLIGVNVATGDRGTVTDINGAYSLSVAADGQL
                     VFSYIGYLSQTIAINGRSVINVTMEEDVRELEAVVKIGYGTVRKRDLTGAVASISAKQ
                     IATIPVPNVAQALQGKMAGVNVITQDGRPDASISIRVRGGGSISQSNEPLVLIDGVPG
                     TLSDVPSDQVESVDVLKDAASTAIYGARGANGVVLVTTKGAKSGKTLVSYNGYAKFNQ
                     PTKYLETLSPYDYVSYVWANADANGINYLDSFTKLFGIGAYGDIERYRNVEAYDVQKK
                     VYNSSFSHNHDLTVSGGTDLTKIILGVNYNDEQGMKINSFHKRASVSLKVDQKINEKM
                     DLGLDTRYVDITSMSDEGTTSGSGSILSYAFRFRPIATSDILGDLNALNEGAVEQYGK
                     FCLWDRYDPYNRIRDYEPLRKRQTLRTIGSFNWELFKGLDYHTDLTLQRSWNQNKFWG
                     GPVHNSYLDDATGEVLHAGAATLYKADSWSYRWSNTLNYEIEINKVNQLSVLLGQEVS
                     NSGGNGMTITADFFPANYTKENAFAMINQYDKAASTNQSPFSSNFMTPSRLLSYFTRM
                     NYSLLDRYLFAFTFRADGSSKFSPNHRWGYFPAGALGWRLSEESFLKDEDWLDNLKLR
                     ASYGSVGNDGISADLWSQNWTAETDDRWYYSIDGVAKPSYDLASSQMANPDLKWETTI
                     TRNAGIDFGVFGNRLTGTLDFYWNTTKDLLMNTVLPGITSFSSTFANIGQTSNKGVEF
                     SLNGVLFENNDWNVEAGFNINFNRNKVDELANAQELIDGKGLYYGTGWFSSGYPGNDF
                     GLYEGKPVGLVRGLVYEGMYSL"
     gene            complement(2863..>3096)
                     /locus_tag="PKY68_08010"
     CDS             complement(2863..>3096)
                     /locus_tag="PKY68_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH25013.1"
                     /translation="DSASVQGKALIAAGYEVAPWGSTIVANEDQYLHDIFKGYTDADY
                     ASKTPPRYLCPMSSTTISKSNGKITNGYGFSQE"
BASE COUNT          865 a          729 c          659 g          843 t
ORIGIN      
        1 ccaacgagta catcccttcg taaacgagac cgcgtactaa accaacgggt ttgccctcgt
       61 acaaaccaaa gtcgttcccc ggatatccgg aagaaaacca accggttccg taatacaagc
      121 ctttgccgtc gatcagctcc tgggcattag ccaattcgtc cactttattc ctgttgaaat
      181 tgatattgaa acctgcttca acattccaat cgttgttttc gaacaatacg ccgttcaaag
      241 agaattcaac acccttattc gatgtttggc ctatattggc gaaggtcgag ctgaaacttg
      301 taatgccggg aagcacggtg ttcatcaaca ggtccttggt tgtattccaa taaaaatcca
      361 aggttcccgt caggcgattt ccgaatacgc cgaaatcaat accggcgttt ctggtaatcg
      421 ttgtttccca cttcaaatcg gggttggcca tctgagaaga ggccaaatcg taagagggct
      481 tagcaacgcc atctatagaa taataccaac gatcatcagt ttctgccgtc caattttgcg
      541 accataaatc ggcgctgatc ccgtcgttac cgacagagcc gtaagaagca cgaagtttca
      601 ggttatccag ccagtcttcg tcctttaaaa aagattcctc gctcaaacgc catccgagag
      661 ctcctgccgg gaaataaccc caacggtgat tcggtgaaaa tttcgaagaa ccgtccgctc
      721 tgaaggtaaa agcaaaaaga taacgatcga gcagactata attcattcgg gtaaaatacg
      781 acagaaggcg tgaaggagtc ataaagttgg aagaaaaagg actctgattg gtactggccg
      841 ctttgtcgta ttggtttatc attgcgaaag cattttcttt cgtgtaattg gcggggaaga
      901 agtcggctgt aatagtcatt ccgtttcctc ccgaattgga gacttcctgg cccaacaaca
      961 cgctcaattg atttacctta ttgatttcaa tttcgtaatt cagggtattc gaccagcgat
     1021 acgaccaact atcggcttta tacaaagtag ccgcacctgc atgaagaact tctcccgtag
     1081 catcgtccaa ataactatta tgaacggggc ctccccagaa tttattctga ttccaggagc
     1141 gttgcagcgt caaatcggtg tgataatcca ggcctttgaa aagttcccaa ttaaaggacc
     1201 cgatggtgcg aagagtctga cgctttctaa ggggttcata atctcttatc cggttgtaag
     1261 gatcgtatct gtcccaaagg cagaattttc cgtactgttc cacggctcct tcgttcaatg
     1321 cattcaagtc tcctaagata tcactagtag cgatcggacg gaaacggaaa gcataagata
     1381 ggatggaacc cgatccggag gtcgtaccct catcgctcat actggttatg tctacataac
     1441 gcgtatccaa accaagatcc attttctcgt tgatcttttg atctactttc aaagaaacgc
     1501 ttgcccgctt atggaacgaa ttaatcttca taccttgttc gtcgttatag ttgactccca
     1561 aaatgatttt agttaaatcg gtacctccgc tcaccgtcaa atcatgattg tgtgaaaacg
     1621 aactgttata aacttttttc tgaacatcgt aggcttctac attgcgatat ctttcaatat
     1681 ctccgtaggc gcctattcca aagagtttgg taaaagagtc tagatagtta atgccgtttg
     1741 catcggcatt ggcccacaca taagaaacat aatcgtatgg actcaaggtt tccagatact
     1801 tggtgggttg attgaacttg gcataaccgt tgtacgacac aagtgttttt ccgcttttcg
     1861 cccctttggt ggttaccaaa acaacaccgt tggcaccacg ggctccgtaa atagcagtag
     1921 atgcggcatc tttgagcaca tcaacacttt caacctgatc cgaaggaaca tcactaaggg
     1981 ttcccggaac accatcgatt aaaacaagag gttcgttcga ctgtgaaatc gagccgccgc
     2041 cccgaacgcg aatggaaata gaagcatccg gacggccatc ttgcgtaatc acattgacac
     2101 cggccatttt tccctgtaat gcctgagcca cattgggaac aggaatggta gcaatttgtt
     2161 ttgcagagat agaggccacc gctcctgtga ggtctctttt gcgaaccgta ccgtagccta
     2221 tttttacgac tgcctccagt tccctgacat cttcttccat ggttacgttg atcacagatc
     2281 ggccgttgat ggctatggtc tgggacaaat aaccaatgta agaaaaaacc aactgaccgt
     2341 cggcagccac cgaaagagaa taagcaccgt tgatatcggt aacagtgcct ctgtctcccg
     2401 tagccacgtt tacaccgatc agcggttcac ctgcggcatc tttgacaaca ccgctcacca
     2461 tttgctcctg cgcaaatgca aacaagcaag tcgcggccag aaatgaggtc acgaatactc
     2521 gtctgaaaaa tgatacaaga ttcataaata cgtgttttga ttgttgataa ttaaggttat
     2581 ttgaaaataa gattaaatta aaaagagatt tctatcaaag tttagtacat aggtatagag
     2641 tttaaggttt gtacatatcg ttttttccat ggttaaggca cacaaatgta aaagattttt
     2701 tttacattat cgcctaacgt ccccaccgtg atagttaata ttacacaatt ttacacccgt
     2761 tatctgctgg ttggtgtgta aaaaaagcgg gattcacgta catgaatccc gcccttgatt
     2821 cataggattt taacctaata agcaagcaaa aatcaccaat tgttattcct gtgaaaagcc
     2881 gtacccgttg gtaattttac cattggattt cgatatcgtg gtcgacgaca tcggacagag
     2941 ataacgggga ggtgttttgc tggcatagtc agcatcggta tatcccttga agatatcgtg
     3001 caggtactgg tcttcgttgg caacaatggt actgccccag ggagccactt cataacctgc
     3061 agcgataagg gccttacctt gcacgcttgc tgagtc
//
LOCUS       DAOSGL010000277         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_29973_length_3077_cov_3.819325, whole genome shotgun sequence.
ACCESSION   DAOSGL010000277 DAOSGL010000000
VERSION     DAOSGL010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29973_length_3077_cov_3.819325"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="rsgA"
                     /locus_tag="PKY68_08015"
     CDS             complement(<1..653)
                     /gene="rsgA"
                     /locus_tag="PKY68_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOH25014.1"
                     /translation="MEGLVIKTAGNVFLVKTLAGDLHECVVKGNFRIKDLSSTNPVAI
                     GDRVLIEKTSDDQTYFITEVLDRSNFIVRKAANLSKQLHILAANIDLAILMVTVNHPQ
                     THTIFVDRFLATAEAYRIPCCLLFNKIDLYGAEDSEYLEALIRLYESIPYPCLKVSAL
                     KGEGIEEVRSLIRGKICLLAGNSGVGKSSLVNRLNPHIQTKVGPISDYHNKGMHTTT"
     gene            complement(661..1194)
                     /gene="frr"
                     /locus_tag="PKY68_08020"
     CDS             complement(661..1194)
                     /gene="frr"
                     /locus_tag="PKY68_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOH25015.1"
                     /translation="MTEENMSQTIDFLTKTLSRIRAGKANVSILDNIRVDYYGTQTDL
                     DKVANLSTPDPKTIAIQPWEKNMIKEIERAIMASDVGITPVNNGEIIRLAVPPLTEER
                     RRQLSRQVKNEGEEAKISIRNARRDTIESIKKLVKEGLSEDIGKNAEAEVQKTHDKFI
                     KSIDEIVALKEKEIMTV"
     gene            complement(1253..1960)
                     /gene="pyrH"
                     /locus_tag="PKY68_08025"
     CDS             complement(1253..1960)
                     /gene="pyrH"
                     /locus_tag="PKY68_08025"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOH25016.1"
                     /translation="MKYKRILLKLSGESLMGKSNYGIDPERLNEYAEQIKELQIMGIQ
                     VGIVIGGGNIFRGLSGSQSGFDRVKGDQMGMLATVINSLALSSALNKLEVSNHVLTAV
                     NMEPVAEHYSTDKAIDFLEKGHVVIFSAGTGCPFFTTDTAASLRAVEIKADLMLKGTR
                     VDGVYTADPEKDPKAVKYDKISFEEIYDKNLKIMDLTATTLCRENNLPVVVFNMDKKG
                     NLVKVLKGENIGTLVHN"
     gene            complement(2003..2758)
                     /locus_tag="PKY68_08030"
     CDS             complement(2003..2758)
                     /locus_tag="PKY68_08030"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOH25017.1"
                     /translation="MKTFTAADLHFVDELSSTNSLLREIWEKEPLLSEAYALASFNQT
                     AGRGQQDNVWVSEAGKNIAYSVFLRPGHLSPASFFVLSQAISLALLKTLGEEIGLQAE
                     IKWPNDIYVDDKKIAGILIENELRGKSILGSIAGIGININQEQFSPNLPNPVSVFQLC
                     GRTFDLVDLTLALHYRLGEAYAKTFTEEGKTEIAAAYHRHLYRKEGFHHYKQGNRPFR
                     ARILRVMPSGQLEVESDEGWIRAFAFKEIEYLI"
     gene            complement(2844..>3077)
                     /locus_tag="PKY68_08035"
     CDS             complement(2844..>3077)
                     /locus_tag="PKY68_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOH25018.1"
                     /translation="MLIVAEVKARSGSEYEEPWQAVDRKKIRRIVQAAHHYIKLHRID
                     LPVRFDILSLLRQGEQWEIEHIEDAFRAPMQTY"
BASE COUNT          693 a          736 c          728 g          920 t
ORIGIN      
        1 aaagtagtag tatgcattcc tttgttgtgg taatccgata tcgggcctac cttggtctga
       61 atgtgcggat tgagcctgtt gaccaaagaa gacttgccca caccggagtt gccggccaga
      121 aggcagattt tacctcttat cagcgagcgc acttcctcta tgccttcgcc tttgagagcg
      181 gatactttca ggcaaggata ggggatggat tcgtagagcc taatcagcgc ttcgaggtat
      241 tcggaatctt cagccccgta gaggtctatt ttgttgaaaa gcaggcagca gggaatgcga
      301 taggcttcgg ccgtagccag aaagcggtct acaaaaatgg tatgagtttg cgggtggttt
      361 acggtgacca tcaatatggc caggtcgata ttagccgcca gaatatgcaa ttgtttggaa
      421 agattggccg ctttgcgtac tatgaaattg ctgcgatcca gcacttcggt aataaagtat
      481 gtctggtcgt cggaggtttt ttcgatcagc acacgatcgc cgatggctac gggattggtg
      541 ctgctcaggt cttttatcct gaaattccct ttaaccacac attcgtgcaa gtccccggca
      601 agagttttga ccaaaaagac attgccggcg gttttgatga ccagtccttc catcgacgaa
      661 ttaaacagtc atgatttcct tttctttgag ggccactatc tcatcgatgg atttgatgaa
      721 tttgtcgtgc gttttctgga cttcggcttc ggcatttttg cctatatctt ccgacaggcc
      781 ctctttaacc aacttcttaa tggattctat cgtgtcacgg cgggcgttgc ggatactgat
      841 tttagcttct tctccttcgt ttttcacctg cctgctcagc tgacggcgcc tctcttcggt
      901 gagcggaggc accgccaggc ggatgatttc gccgttgtta accggggtga tgcctacatc
      961 ggaagccata atggctcttt cgatttcctt tatcatattt ttttcccaag gctgtatagc
     1021 gatggtcttg ggatccggag tactcaggtt ggccactttg tccaaatccg tctgcgtacc
     1081 gtaataatcg acgcggatgt tatccagaat actgacattg gccttgccgg cacgaatccg
     1141 tgaaagggtt tttgtcagaa agtcgatggt ttgagacata ttctcttcgg tcatgctcag
     1201 gtaaagatcc aattcttcgt tcataggtat agtcttaaag tgtttgctga aattaattat
     1261 gaaccagggt gccgatattt tctcccttaa ggacttttac cagattccct tttttgtcca
     1321 tgttaaagac aacgacaggt aaattgtttt cccggcaaag agtggtagca gttaagtcca
     1381 ttattttaag gttcttgtcg tagatttctt caaagctgat cttgtcgtat ttaacagcct
     1441 tggggtcttt ttcgggatcg gccgtatata ctccgtccac gcgggtgccc ttaagcatca
     1501 gatcggcttt tatttctacg gcacgcagcg acgctgcagt gtcggtggtg aaaaacgggc
     1561 aacccgtgcc ggccgaaaaa atgaccacat ggcctttttc gagaaaatca atggctttat
     1621 cggtgctgta gtgttccgct accggctcca tattgacagc cgtaagcacg tggttggata
     1681 cctccagttt attcaaagca gaactcaggg ccaggctgtt tatgacagtg gccagcatac
     1741 ccatctgatc gcctttgaca cggtcgaatc cactctggga accgctcaaa cccctgaaaa
     1801 tatttccacc gcctatcact atgcccactt gtatgcccat gatctgcagc tctttgatct
     1861 gttcggcata ttcgttgagg cgctcgggat ctatcccgta gttgcttttc cccatcaggg
     1921 actctccact gagtttcaat aatattcttt tgtatttcat gctgatcttg tcctggacgc
     1981 acaaaagtac attttttttg cgctaaatca aatactctat ttctttaaat gcgaaggctc
     2041 tgatccatcc ctcgtcgctt tcaacttcca actgcccgct tggcatcacc cgaagtattc
     2101 tggccctgaa cggtctgttc ccctgtttat agtggtgaaa accttctttg cggtaaagat
     2161 gtcggtggta agctgctgca atttctgttt ttccttcttc cgtgaaggtt ttggcataag
     2221 cctcacccag ccggtagtgc agtgcaagcg tcaggtcgac taagtcaaaa gtccgcccgc
     2281 acaactgaaa gacggaaaca ggattgggca ggtttgggct gaattgctcc tggtttatat
     2341 ttatacctat accggcaata ctgcccagaa tggattttcc ccgtagttcg ttttcaatca
     2401 aaataccggc tatttttttg tcatccacat aaatatcatt gggccatttg atctcggcct
     2461 gcaggccgat ttcctcgccg agtgttttca acagagccag tgaaatcgcc tgggacaata
     2521 caaagaaaga ggccggacta aggtgtccgg gacgaagaaa aacactgtac gcaatatttt
     2581 tgcctgcttc cgatacccag acattgtctt gttgtccccg gccggctgtc tgattaaagc
     2641 tggccagagc ataagcttcg ctcaacaggg gctctttctc ccatatttcc ctcagcaaag
     2701 agttggtaga gctcagttca tccacaaagt gaaggtcggc tgcagtaaag gttttcaaag
     2761 tctttgtcgg gtttagtgat taataagttt gaatgggagc atggctcgta ccgtcttatc
     2821 gtctttttga ttcagtttag caattaatag gtttgcatgg gagctctgaa ggcgtcttcg
     2881 atatgttcta tttcccattg ctctccctgc ctgagcagcg acaaaatatc aaagcgcacc
     2941 ggcaggtcga tccggtggag ctttatataa tggtgggcag cctgaacgat acggcgtatt
     3001 tttttacgat ccactgcctg ccagggttcc tcgtactcgc tgccgctgcg ggcttttact
     3061 tcggctacta tcaacat
//
LOCUS       DAOSGL010000278         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_30580_length_3033_cov_3.838818, whole genome shotgun sequence.
ACCESSION   DAOSGL010000278 DAOSGL010000000
VERSION     DAOSGL010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30580_length_3033_cov_3.838818"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PKY68_08040"
     CDS             <1..1379
                     /locus_tag="PKY68_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH25019.1"
                     /translation="PLGVGLIGLYQSIIEMIRSVSGLGLHFSSVRDMAQANAGGDRQQ
                     INTTASVLTRWIVFTAILGALLCVVFSKQISTLAFGDSSKSSQICLLSFSVFALTLSA
                     GQKSMLQGMRKIAYMAKASVLGSLSGFIVASILYLTLGQEGIVPALLAAAFLSLVFTW
                     WYRHKLSMQRVPVSLRETYLRGKKMVALGLFTMLSGVISTLTMLLLKSQIGRWADLDT
                     VGLFQSSWSLTAIYLSAILSAMSADYYPRLCEVSSDNARMVRYVNEQLHVVLLAATPL
                     VVGMLLFAPWVLHLFYSAKFVPAEGLLRWQIAGSFLKVLSWPFGYILLSRSKGLLFLL
                     TELLWHGSYLAFTWLLWEPLGLTAAGLAFLLAYVLYTLCLLLLARRICDFRIQSRNLY
                     FLGLFLFFVLSAFLLAHVFHPVYWIKWSLSALILLVCSCVSLQELNRIWPLKDLWASL
                     KKRISKDD"
     gene            1372..2352
                     /locus_tag="PKY68_08045"
     CDS             1372..2352
                     /locus_tag="PKY68_08045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH25020.1"
                     /translation="MTKPLVSVLILAYNQEEYIADCLDSALAQQGDFDFEIVVAEDAS
                     VDGTLVICREYQRRYPGKIVLLSNQSNRGVVRNYFNALEHCRGEFIADCGGDDYWPDP
                     LRLQKQWKLLVGDPAMSMVYGQWKEQAAESTGTAQNKETSWPTLPQYDPTDFGPQTAA
                     RFLNRVYKPEIVLSTAMYRKSFVLEALRQKPELFLHPDYRTEDLSVLTASLLRGPACY
                     LPECFLVYRVLDNSVSHPRQMDKYYRFALSSFRQSLDWARHIGLSLGEILSYLQSRYN
                     DYMHYAFTSCDKQLARALRKSVREYAYRPSLKNEIKYYLTCCSLKKPKAL"
     gene            2349..>3033
                     /locus_tag="PKY68_08050"
     CDS             2349..>3033
                     /locus_tag="PKY68_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25021.1"
                     /translation="MKILFLLPEYPALGGVQTVTTQMANYFQAAGHEVSILSLPPGKW
                     YGLPGLSSPRPAGADFPRQSSGADSLRQSPCSDSRHSQTLPPEKPLRITLDPGVKQAY
                     LPDSSDYNSKQNLQFIRDYVEQNRIPIIINQGAFSKVYLLFSEHSEHKVINVLRGCPA
                     WEPVRKRKFNTFKNTVLSVRGVGKRIPALLRYLAMKTVPGLATGLARRELFAKIVHAA
                     RFVVLAPGYI"
BASE COUNT          645 a          734 c          782 g          872 t
ORIGIN      
        1 ggcctctggg tgtgggactg atcggtttgt atcagtccat catcgaaatg atccgctcgg
       61 taagcggcct gggtttgcat ttcagttcgg tcagggatat ggctcaggcc aatgccggcg
      121 gcgaccggca acaaatcaat actaccgcat cggtactgac cagatggatc gtttttacgg
      181 ctattttggg agccctgctt tgcgtggtgt tttcaaaaca aatcagtacg ctcgcgttcg
      241 gcgacagcag caaaagctct caaatttgcc tgctgtcttt ttctgtattt gctctgactc
      301 tttcggcggg ccaaaagtcg atgctgcaag gcatgcggaa aattgcctat atggccaaag
      361 cttccgtcct gggcagcctg tccggtttta tagttgcttc gatcttgtat ttgacgctgg
      421 gacaggaggg cattgttccg gctctgttgg cggctgcttt tctgagcctg gtgtttacct
      481 ggtggtatcg gcataagtta tccatgcagc gagtccctgt aagtctcagg gaaacctatc
      541 tacggggaaa gaaaatggtt gccctgggtt tgtttaccat gctgtcgggc gtgatcagta
      601 ccctgaccat gttgttgctc aaaagccaga tcgggcgttg ggccgacctg gataccgtgg
      661 gtttatttca gtcttcctgg tctttgacgg ccatttacct ttcggccatt ttgagcgcca
      721 tgtcggccga ttattatccg cgtctttgcg aagtaagcag cgataacgcc agaatggttc
      781 gttatgtgaa cgaacaactg cacgttgtgc ttttggcagc tacgcctttg gtggtgggta
      841 tgttgctctt tgccccgtgg gtgttgcatt tgttttattc ggctaaattt gtacccgccg
      901 aaggcttatt gcgctggcag attgccggca gttttctcaa agtgctgagt tggccctttg
      961 gttatatttt gctcagccga tccaaaggct tgctgttttt gttgactgaa ctgctttggc
     1021 acggctctta cctggctttc acctggctgt tatgggaacc cttgggcttg actgccgccg
     1081 gtttggcctt tttattggcc tatgtgttgt acaccctttg tttgctcctg ttggcccgtc
     1141 gcatttgcga tttccggata caaagcagga acctgtattt tttgggcctc tttcttttct
     1201 ttgtgctctc ggcttttttg ctggctcatg tattccatcc tgtctattgg atcaaatggt
     1261 cgctttcggc cctgatattg ctggtttgca gttgtgtttc cttgcaggaa ctcaatagaa
     1321 tctggccgct taaagacttg tgggcaagct taaaaaaacg tatttcgaaa gatgactaaa
     1381 cctttggtgt ccgtattgat tttggcctat aatcaggaag aatacatcgc tgattgtttg
     1441 gattcggccc tggctcaaca aggggacttc gattttgaaa tagtagttgc cgaagatgct
     1501 tccgtcgacg gcactttggt gatttgccgg gaatatcaac ggcgttatcc cggcaaaata
     1561 gtgctcctga gcaatcaaag caatagggga gtggtgcgga attattttaa cgccttggaa
     1621 cattgccggg gagaatttat agccgattgc ggcggagacg attactggcc cgatcctctg
     1681 cgcttgcaaa agcaatggaa acttcttgtt ggggatccgg ctatgagcat ggtttatggg
     1741 caatggaaag aacaggcggc cgaaagtact ggaaccgcac aaaacaaaga gacctcttgg
     1801 ccgacccttc cacaatacga tcccaccgac ttcggcccac agacggcagc ccgtttttta
     1861 aaccgcgtgt ataaaccgga gattgtgctt tcaaccgcca tgtacagaaa aagtttcgtg
     1921 ctggaggctt tgcgacaaaa gcccgaactg tttttgcatc cggattaccg caccgaagac
     1981 ctttcggtgc tcacagcctc gttgttgcgt gggcctgcct gttatttacc ggaatgtttt
     2041 ttggtttacc gggtgctgga taattctgtc agtcatcccc ggcaaatgga caagtattat
     2101 cgttttgccc tgagcagctt caggcaaagt ctcgactggg ccaggcatat agggttgagc
     2161 ctcggggaaa tactttctta tttgcaaagc agatacaacg attatatgca ctatgccttt
     2221 acctcgtgcg acaagcaatt ggcccgggca ttgagaaagt ccgtcagaga gtatgcttac
     2281 cgtccctctc ttaaaaatga aatcaagtat tacctcacct gttgttccct aaaaaagccc
     2341 aaagccctat gaaaatattg tttctgcttc cggaatatcc tgccttgggt ggggtgcaga
     2401 cagtgaccac ccagatggcc aattattttc aggctgccgg ccacgaagtg agcattctga
     2461 gtctgcctcc gggaaaatgg tacggattgc ccgggctttc gtctccccgg ccggccgggg
     2521 ctgattttcc ccggcagtca tccggggctg attctctcag gcagtctccc tgctccgatt
     2581 caaggcattc gcaaaccctg cctcccgaaa aacctttgcg gataactctc gatccgggag
     2641 tcaaacaagc ttatttgccc gattcttccg actacaattc aaagcagaat ctccaattca
     2701 tacgcgatta tgtagaacag aaccggattc ctatcatcat caaccagggc gccttttcca
     2761 aagtttattt gctgttctct gagcattcgg agcataaggt gatcaatgtg ctcaggggct
     2821 gtccggcctg ggagccagtg cgcaaacgca agtttaatac gttcaaaaat accgtgttgt
     2881 ctgtacgcgg tgtcggcaaa agaataccgg ctttgttgcg ctatctggcc atgaaaacag
     2941 tacccggtct ggcaactggc ctggcccgta gggaattatt cgccaagatt gtacatgctg
     3001 cccgttttgt ggtactggca ccgggttata tcg
//
LOCUS       DAOSGL010000279         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_30680_length_3026_cov_3.124201, whole genome shotgun sequence.
ACCESSION   DAOSGL010000279 DAOSGL010000000
VERSION     DAOSGL010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30680_length_3026_cov_3.124201"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PKY68_08055"
     CDS             <1..503
                     /locus_tag="PKY68_08055"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOH25022.1"
                     /translation="DSQVILLTSSAALAEAVQKEIQTQCAALPRATIAWKALENSKII
                     LLKDREEMLRFSNAYAPEHLIISTKDYRRLADRVTNAGSVFLGNYSPESAGDYASGTN
                     HTLPTKAYARAYSGLNLDSFKKKISFQEISREGLQALGPTIEIMAESEELLAHKRAVS
                     IRLKQD"
     gene            516..1568
                     /gene="hisC"
                     /locus_tag="PKY68_08060"
     CDS             516..1568
                     /gene="hisC"
                     /locus_tag="PKY68_08060"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOH25023.1"
                     /translation="MSTLPLEQLLRKNILELTPYSCARDEFQGQASVYLDANENPYNG
                     PYNRYPDPLQRELKQKIAAIKGLRSGQVFLGNGSDESIDVLFRAFCEPRTDNVVAIEP
                     TYGMYRVSAGINDVAYRAVLLDELFQIDAGRILAACDARTKLVFLCSPNNPTGNSLNR
                     AEILKLLDRFEGILVLDEAYIDFASEPSLMSRLDDYPGLVVLQTFSKAWGSAAIRLGM
                     ALASESIVKVLNKIKYPYNVNLLTQQQAAKMLESAPRVKDWVAQILQERERLRKALSE
                     LRLVKHIYPSDANFLLVRVSDANRLYTALTEQGIIVRNRNNVSLCLGCLRITIGTAGE
                     NQILLQALKALDQNFG"
     gene            1597..2718
                     /gene="hisB"
                     /locus_tag="PKY68_08065"
     CDS             1597..2718
                     /gene="hisB"
                     /locus_tag="PKY68_08065"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOH25024.1"
                     /translation="MKKRALFIDRDGTIIEEPKPSLQVDNLDQLVFLPKVIRNLYFIR
                     KKLDFEFIMVSNQDGLGTDAYPTENFERVQNKMLQILQNEGICFDAIHIDPSLPEQNS
                     PGRKPGTAMLTSYMDGSYDLAASFVIGDRMTDVQLAHNLGCKCILLNEEINLQESKEY
                     AASCVMQSTDWDKISAFLFAGQRKVSIERKTKETEVKLELDLDGSGKTNIDTGIKFFD
                     HMLDQIGRHSGCDLSIIVKGDLEVDEHHTVEDTALVLGEALRQALGDKRGIERYGFCL
                     PMDDSLCSLALDFGGRPWLVWDVTFRREKLGELPTELIFHFFKSLSDSAKMNLNIKAL
                     GDNEHHKSESIFKAFALALKMAIRRDIYRYDLPSTKGLL"
     gene            2790..>3026
                     /locus_tag="PKY68_08070"
     CDS             2790..>3026
                     /locus_tag="PKY68_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25025.1"
                     /translation="MKRIFFSLGLLTLVACPLFAQTSNLGLAADQPQEQPSRFPTELE
                     VSLSPKDIVYATKDTSVLKMDIYRPQAQDKALPCL"
BASE COUNT          820 a          743 c          707 g          756 t
ORIGIN      
        1 acgacagcca ggttatcttg ctgacctctt ctgccgcttt ggccgaagcc gtacaaaaag
       61 aaattcagac ccagtgtgcc gctctgccac gtgcaactat tgcatggaaa gcattggaaa
      121 acagtaagat tattctcctg aaagaccggg aagaaatgtt acgtttcagc aacgcttacg
      181 ctcctgagca cctgattatt tccacgaaag attaccgccg gctggccgat cgtgttacca
      241 atgcaggttc cgttttcttg ggcaattaca gccccgaaag cgccggcgat tacgcttcgg
      301 gcaccaatca cacgcttccc accaaggctt atgcccgggc ctacagtggt ttgaacctgg
      361 atagtttcaa gaaaaaaatc agttttcagg aaatcagccg cgaaggttta caggccctag
      421 gcccgactat cgagatcatg gccgaaagcg aagagcttct ggcacataaa cgagccgtga
      481 gtatacgtct taaacaagat taattttaac ccattatgtc aacgctcccc ttagaacaat
      541 tgctccgcaa aaacatacta gagcttactc cctattcctg cgcccgcgac gagtttcagg
      601 gtcaggcatc ggtgtatctc gatgccaacg aaaacccata caacggaccc tacaaccgct
      661 atcccgatcc tttgcagcgg gagcttaaac agaaaatcgc agctatcaag ggattgcgct
      721 ccgggcaagt gtttttgggc aacggcagcg atgaatccat cgatgtcttg ttcagggctt
      781 tttgcgaacc ccgcacagac aacgtggtag ccatagagcc tacctacggc atgtacagag
      841 tttccgccgg aatcaacgat gtagcctacc gtgccgtctt actcgacgaa ctttttcaaa
      901 tagatgccgg cagaattttg gccgcctgcg atgcccgtac taagcttgtt tttctttgtt
      961 ctcccaacaa tcctacgggc aacagcctga acagagccga aatcctgaaa ttgctggacc
     1021 gattcgaggg gatactggtg ctcgacgaag cctacattga ttttgcttcg gagccttccc
     1081 tgatgagccg gctggacgat tatcccggtc tggttgtgtt acagacattt tcaaaagcct
     1141 ggggcagcgc tgccatccgc ctgggtatgg ctctggcctc tgagtcgata gtgaaggtgt
     1201 tgaacaagat taaatacccc tacaacgtta acctgctcac ccagcagcaa gccgcgaaga
     1261 tgttggaatc tgctccccgg gtgaaggatt gggtagcaca aatcctgcag gaacgtgaac
     1321 ggctgcgcaa agccctgtca gaactccgtt tggtcaaaca catttaccct tcggacgcca
     1381 actttttgct ggtaagggta agcgatgcca atcgacttta tactgctttg acagaacaag
     1441 gcatcattgt gcgcaacagg aataatgtat ctttgtgtct gggttgcctt cgaataacga
     1501 taggcacagc cggggaaaac caaatcctgc tacaagcttt aaaagccttg gatcaaaact
     1561 tcgggtagtg ttttgtatat aaataataga attccaatga aaaaaagagc cttgtttatc
     1621 gatcgtgacg gaacaataat cgaagaacca aagcccagcc tgcaggtgga caatttggat
     1681 caattggtgt tcttgcccaa agtaatccgg aatttatact ttatacgcaa gaaactggat
     1741 tttgagttta ttatggtgtc caatcaagac ggcctcggca cagatgcgta tcccacagaa
     1801 aatttcgaaa gggtgcagaa taagatgtta caaatcctgc aaaacgaagg catttgtttt
     1861 gatgccattc acatagaccc cagtttgccc gaacaaaatt cacccggccg caaaccggga
     1921 accgccatgc ttacatccta tatggacgga agctacgatc tggctgcttc ttttgtaata
     1981 ggagacagga tgacggatgt tcaacttgca cataatctgg gttgtaagtg tatattgtta
     2041 aacgaagaga taaaccttca ggaatctaag gaatacgccg caagctgtgt tatgcaaagc
     2101 acggactggg ataaaatcag cgcttttctt tttgccggcc agcgcaaggt cagcatagaa
     2161 cgcaagacca aagaaaccga ggtgaagttg gaactcgatt tggacggtag cggtaaaacc
     2221 aatatcgata cgggcattaa attttttgat cacatgctcg accagatagg gcgacattcg
     2281 ggttgcgatc tgagcataat tgtcaagggc gatttggagg tggacgagca tcatacggta
     2341 gaagataccg ctctggtttt gggcgaggcc ttgcgtcagg ccttgggcga caaacgagga
     2401 attgaacgtt acggtttttg tttgcccatg gacgatagtc tttgtagtct ggccttggat
     2461 tttggcggcc gcccctggct ggtttgggac gtgactttcc gcagagagaa gctgggagaa
     2521 ctccccacgg agctgatttt tcacttcttc aaatccttga gcgattctgc caaaatgaat
     2581 ctgaacatca aagctttggg tgacaacgaa caccataaat cagaaagcat attcaaggca
     2641 ttcgcattag cactcaaaat ggccatacgc cgcgatattt atcgttacga tttacccagt
     2701 accaaaggcc tgctttaatc tatttgctct gtgctttttc aaataatttg ctacctttga
     2761 cacctccggt tcactaaata ccaaagcaaa tgaaacgtat ttttttcagc ttaggcttgt
     2821 tgaccctcgt cgcttgtccc ttatttgccc aaacctcaaa cctgggcctg gcagcagacc
     2881 aaccacagga gcagccctcc agatttccta ctgaactcga agtaagcctt tctcccaagg
     2941 atattgtgta cgccacaaaa gatacctcgg tactcaaaat ggacatttat cgtcctcagg
     3001 ctcaggataa agctttgccc tgcctg
//
LOCUS       DAOSGL010000280         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_30768_length_3020_cov_3.570995, whole genome shotgun sequence.
ACCESSION   DAOSGL010000280 DAOSGL010000000
VERSION     DAOSGL010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30768_length_3020_cov_3.570995"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..901)
                     /gene="rlmB"
                     /locus_tag="PKY68_08075"
     CDS             complement(131..901)
                     /gene="rlmB"
                     /locus_tag="PKY68_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOH25026.1"
                     /translation="MNRRFNIRKRPPGGGNEMVFGIRAVMEALHAGKEIDKIWIRNNL
                     EGELSRELSEVLKGYEIPVQRVPQEKLIHLSSKNHQGVVAFISPITYQRLDQLIPSLY
                     DQGIMPFVVVLDGITDVRNFGAIARTCSCAGVHAIVVSQTGSVSVNADAIKTSAGALY
                     RLPVSRERNLKESLHFLKLSGIKLIGASEKADKDYRTADYSGPLALILGAEDQGIAPA
                     HLSLCDEIVRIPVLGEIASLNVSVAGGVLMYEALRHRD"
     gene            complement(903..2558)
                     /gene="recN"
                     /locus_tag="PKY68_08080"
     CDS             complement(903..2558)
                     /gene="recN"
                     /locus_tag="PKY68_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOH25027.1"
                     /translation="MLSRLHIENYVLIEHLDLQFESGFSVITGETGAGKSVLMGAISL
                     ILGQRADLRLIKEGASRCVVEAEFNQMQHLSDFFARQGMEFDPESCIIRRELYENGKS
                     RAFVNDSPVALNTLRDLAIKLVDVHSQHESLLLDNSLFQLELLDNLAQSREELTAYRL
                     VYENYRQASQELKTLEESLQKDIDEQDYLLFQVEQLRSAAFRNGEQEQLEQELSILDH
                     AEEIKTSFSKIENLLSDEEQGICGRLKMAVQETSSLRSLWPKMADAHERIHSAYIDLN
                     DLQRDIARWGNDVEYNPQQRDLVQERLSFLYELLQKFKARNLDELLSKQTELEERIAF
                     IEQGQELLNAARQKRDAALQAARQKAEVLSGKRKARCKEIENHLRVQLQQLGMPHGVF
                     NIEVEEKALDEQGMDKVTFLFSANKNIAPQPIGQIASGGEISRLMLSLKALVASRGNY
                     SCLLFDEIDAGVSGEIAHKMAALMQETALGRQVICITHLPQIAARGQQHYKVFKTEEG
                     EQALSKVIRLNEEERVTEIALMLSGANLSQAAINNARHLIYGE"
     gene            complement(2563..>3020)
                     /locus_tag="PKY68_08085"
     CDS             complement(2563..>3020)
                     /locus_tag="PKY68_08085"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOH25028.1"
                     /translation="MDFDSFAPYGGERFLTKASDIVSQAQGFLGDNWKANEDDKNCWG
                     WTNALNDENQKPMRLLNYNYHRQGMDLLHQDAAAARKKITDSFKVLEEVKRLNPSSPL
                     LSNLADSKIEEWIQLYSKAESKEKQEVFDLLSFIYPGQSNRLQAIKNQN"
BASE COUNT          726 a          759 c          699 g          836 t
ORIGIN      
        1 acctgagtct gatggacaat ctttaccagg tggaagcctt gcaggcagtc gatatgtttc
       61 cccatacccg gcacgtggaa aacgtggtgc tgttgaaacg cagacaatag gctgttcagt
      121 cgtgtgttgc ttaatcgcgg tgacgcagcg cttcgtacat cagaacgccg ccggccacag
      181 atacattgag cgaagcaatt tctcccaaca ccggaattct gacaatttca tcgcagagcg
      241 acaaatgcgc cggagcgatg ccttgatcct cggctcccag aatcaaagcc aaagggccgg
      301 aataatcggc agtcctgtag tctttatcgg ccttttcgct ggctccgatc agttttatac
      361 ccgataattt caggaaatga aggctttctt tcagattgcg ctcccgggaa acaggcaaac
      421 gatacaaagc gccggcagaa gtcttgatgg catcggcgtt aacactcaca ctgccggtct
      481 gagacacaac gatggcatga acgccggcac aggagcaggt tcgggcgatg gcgccaaagt
      541 tacgaacgtc ggtaataccg tcgagcacca ccacaaaagg catgatgccc tgatcgtaca
      601 aagagggaat cagctgatcc agacgttgat aagtgatggg tgaaataaaa gccaccaccc
      661 cctgatggtt tttggaactc aggtgtatca atttttcctg gggaacccgc tgtacaggga
      721 tctcatagcc cttgagcact tcgctgagtt cgcgcgaaag ctcgccttcg agattgttac
      781 gtatccagat tttatcgatt tctttgccgg catggagcgc ttccataacg gctctgatgc
      841 cgaaaaccat ttcgttgcct ccccccgggg gacgttttct gatgttgaac cgacgattca
      901 ttctattctc cgtatatcag gtgacgggcg ttgtttatag cagcctggct caaattagcg
      961 ccgcttagca tcagggctat ttccgtaacc ctttcctctt cgttaaggcg tataacctta
     1021 ctcagggctt gttcgccctc ttcggtttta aatactttat aatgttgctg accacgggca
     1081 gcgatttgcg gcagatgagt aatgcaaatg acttgccgtc ccagggcggt ctcttgcatc
     1141 aaagcggcca ttttatgggc aatttccccg gaaacgcccg cgtctatttc atcaaacaac
     1201 aaacaggaat aattgcccct gcttgccacc aaagccttaa ggctcagcat caggcgtgat
     1261 atttcacctc ccgaagcaat ctgaccgatg ggttgcggag ctatgttttt gttggcagag
     1321 aacaaaaagg tcactttgtc catgccctgt tcatcaaggg ccttttcttc tacttcgatg
     1381 ttgaaaacac cgtgaggcat tcccaactgc tgcaactgga ctctgagatg gttttcgatt
     1441 tccttacaac gggcttttct cttgcccgat aaaacttcgg ctttttgacg ggcagcttgc
     1501 aaggccgcgt cgcgcttttg tctggcggcg ttgagcaatt cttgtccttg ctcgataaaa
     1561 gcgattcttt cttccaactc cgtttgttta ctcaacaatt catcgaggtt ccgggccttg
     1621 aatttttgca gcaattcgta aagaaaactc aggcgttcct gcaccagatc tctttgttga
     1681 ggattgtatt caacgtcgtt gccccaacgg gctatatcgc gctgtaaatc gtttaaatcg
     1741 atgtaagcgg aatgaattct ctcgtgagca tcggccattt tcggccatag tgaacgcagc
     1801 gaagacgttt cctgcacggc catcttcagc cgaccacaaa tgccctgctc ctcgtcggaa
     1861 agcagatttt ctattttact gaaggatgtc ttgatttctt cggcatgatc cagtatggat
     1921 aattcctgtt ccagttgttc ctgttcgccg tttctaaatg cagcagagcg caattgttcc
     1981 acttgaaaaa gcagataatc ctgttcatca atatccttct gcaggctttc ttccagcgtt
     2041 ttcaattcct gagaggcctg acggtaattt tcgtacacca agcgataggc tgtcaattcc
     2101 tcccggcttt gagccaggtt gtccaacaac tccagttgaa acaaactgtt gtcgagcaaa
     2161 aggctttcat gttgagagtg tacgtcaacc aattttatgg ccaggtcacg caacgtgttc
     2221 aaagccaccg gcgaatcgtt gacaaaagca cggcttttgc cgttttcgta caattccctg
     2281 cggataatac aggattcggg atcgaattcc ataccctgtc gagcaaaaaa atcggaaaga
     2341 tgctgcattt ggttgaattc agcttcgacc acacaacgcg aggctccctc tttgataagc
     2401 cgtaaatcgg ctctttgccc aaggatcagg gaaatggctc ccatcaatac cgatttacca
     2461 gccccggttt cgccggtgat aacggaaaat ccggattcaa attgtaaatc caaatgttcg
     2521 atgagtacgt aattttctat gtggagcctg gacaacatac aatcagtttt gatttttgat
     2581 ggcctgtaaa cggttgcttt gtccggggta tataaaacta agcaggtcga agacctcttg
     2641 tttttctttg ctttcggctt tggaataaag ctgtatccat tcttcgattt tagaatcggc
     2701 caaattgctc agtaatggtg agctggggtt caatcttttt acttcttcca gcaccttgaa
     2761 agagtcggtg atttttttac gagcggccgc agcatcctga tgcaaaagat ccatgccctg
     2821 gcggtggtag ttataattaa gcagtcgcat cggtttttga ttttcatcgt tcaaggcatt
     2881 ggtccagccc cagcagtttt tatcgtcttc gttcgccttc cagttatcgc ccaaaaaacc
     2941 ctgagcctga gatactatat cactggcctt ggtcaaaaac cgttcgcctc cgtagggagc
     3001 gaaactgtca aaatccatgc
//
LOCUS       DAOSGL010000281         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_31341_length_2981_cov_4.234108, whole genome shotgun sequence.
ACCESSION   DAOSGL010000281 DAOSGL010000000
VERSION     DAOSGL010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31341_length_2981_cov_4.234108"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="PKY68_08090"
     CDS             <1..1346
                     /locus_tag="PKY68_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH25029.1"
                     /translation="GNSWYEPGDTTWHEPGDTTWYEPGDTTWYEPGDTTWYEPGDPEN
                     AWDTAQIKLWYPDRQVNNTEVFSVLLHTSMLDPTLGIIAYQFDLYYDNNAMEYQGYDI
                     ENSISAGGSILVNANDTGVLHVSWMKQDTLSGMGLLVKLDFKAVNYGYTYCSISDFFF
                     NTYSIYDIENGDIEIMELIYGDVDGNYMVQAYDAAIVLQHSVGLDPLPAVDPLPWENW
                     RLLRADVDEVEGITANDAAFILQYSAKLINTFPVESLMRSAQNNGASISITQEDNQLV
                     FRSYGDMIGLNIQAFGDLSVLGVPEIMDKNMISATNINGELYSVGLARAYASEDGETF
                     MIIPLMSDDYAEISFETIVNTETQTYYNQVGTGLAVKAETEKITCVNNGRQLFLYNLP
                     TKAQISIYGITGQLMSTKTTRSANETLDIQALNKGIYLIRIENQGQTQTFKFIRN"
     gene            complement(1449..2717)
                     /locus_tag="PKY68_08095"
     CDS             complement(1449..2717)
                     /locus_tag="PKY68_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25030.1"
                     /translation="MRTKISLLACLLCLAACQYNNDREHEQDQNQGLLIKGQIPQRTK
                     AGLTRSADSLSLEDADKVLVLSYWGNQFIDIDSGRFSIYPEWGTAIALLFLDSAYRYI
                     GHLSTRGLNMLPLGNLSEGENTLIDLSMLRLEGHTVIPGYDPFGNEILLSEQELQALQ
                     SLGEYYNAIAYNMDADRDGQPDCLSGKGLMMSTIVVYPRCGRFGQNDRLPEVEPDSSC
                     VNYTLQISSGPDLQFSDNNISFYGPEGEPYDDMQLNYIIEEGETLFNAYISRPNGQIR
                     EGACCGDLLLPFKKGVYTLKLDELEYKLIYANNQYYSALVLAVPSIITNGDTLKSIRL
                     EYQLTNGTKVEPSAMLNILYAQITDSSGMHDIPHVTGSDCSDWLSIRTGFDSIVPAGS
                     NILLREIQHVSVWYDDILGNHYALIFPNAY"
     gene            complement(2778..>2981)
                     /locus_tag="PKY68_08100"
     CDS             complement(2778..>2981)
                     /locus_tag="PKY68_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25031.1"
                     /translation="IRMPREVFNKSTQFPRLGVGKLISVEEQSRYGNTEDFGALLRKS
                     VYDMPRPIQFIPREHLFSDRLFA"
BASE COUNT          841 a          671 c          675 g          794 t
ORIGIN      
        1 acggaaattc ttggtatgaa cccggggaca ccacctggca tgaacctggg gacactacct
       61 ggtacgagcc cggagacact acctggtacg agcccggtga tactacctgg tacgaacccg
      121 gagatcctga aaatgcctgg gataccgccc aaatcaagtt gtggtatcct gacagacagg
      181 ttaataatac agaagttttc agtgttctgc tccacaccag catgctcgat ccgaccctgg
      241 gcatcatagc ctatcaattt gatttatact acgataataa tgccatggaa taccagggct
      301 acgacataga aaacagtata tcggcaggtg gcagcatttt ggtcaatgcc aacgacaccg
      361 gagtactgca cgtcagctgg atgaagcagg ataccctgag cggaatgggg cttctggtca
      421 agctggactt taaggcggtc aattacggat atacttattg ttctatttcg gatttcttct
      481 ttaacactta ttcaatctat gacatcgaaa acggagacat tgagattatg gaactgattt
      541 acggcgatgt ggatggcaat tatatggtac aagcttacga tgcagctata gttctgcaac
      601 attccgtagg gcttgatcct ttgccggctg tggatcctct tccctgggaa aactggagat
      661 tgctgcgtgc agacgtagat gaggtagaag gcattaccgc caacgatgcg gcatttatac
      721 tacaatattc cgccaaactc atcaacactt tcccagtgga aagtcttatg agaagcgcgc
      781 agaacaacgg agcctccatc agtatcactc aggaagataa tcaactggtt ttcagatcct
      841 acggagacat gattggtctt aatattcagg cgttcggcga tctgtcggtc ttgggtgtac
      901 ccgaaataat ggacaaaaac atgatctcgg ctaccaacat caacggagag ctatattctg
      961 tgggcttagc ccgggcctat gcttccgaag acggggaaac ctttatgatt atacccctga
     1021 tgtcggatga ttatgccgaa ataagttttg aaaccatagt gaatacagaa acacagactt
     1081 attacaacca ggtcggtacc ggtctggctg ttaaagcaga aacggaaaaa atcacttgtg
     1141 taaacaatgg ccgccagctc tttttgtaca acctgccgac taaagcccaa atcagcattt
     1201 atggtattac cggacagttg atgagtacaa aaactactcg atctgccaac gaaaccctcg
     1261 acattcaagc gctgaacaag ggaatctatc tgatacggat tgagaatcaa ggacagactc
     1321 agacatttaa gttcatcaga aactgaatct aaaactgtac cgagtttcat ttaaagcaag
     1381 agtgtctcat aactaaaagt tttgagacac tctttttttg cttaactaaa ctcctcagga
     1441 gattttcttc aataggcatt gggaaaaatc aaggcataat ggttgcccaa aatatcgtcg
     1501 taccaaacac tcacatgttg aatttcgcgg agcagaatat tgctgccagc gggaacaatg
     1561 gaatcaaaac ctgtccggat gctcagccag tcggaacagt cggagccagt aacatgagga
     1621 atgtcgtgca ttccgctgct gtcggttatt tgagcgtaga gaatattcaa catggccgag
     1681 ggctcaactt tggtgccgtt tgttagttga tattccagcc tgatagactt tagggtatcg
     1741 ccattggtga taatgctggg aactgccagc accagagcgc tataatattg attgttggcg
     1801 taaatcagtt tgtattcgag ttcatccaac tttagcgtat acactccttt tttgaaaggc
     1861 agcaaaagat ccccacaaca agctccttcc cggatctgcc cattaggcct gcttatatag
     1921 gcattgaaca gtgtttcacc ctcttcgata atataattaa gctgcatatc gtcgtaaggt
     1981 tctccctccg gaccgtaaaa agatatatta ttatcactga attgtaagtc cgggcccgaa
     2041 gatatttgta aggtatagtt gacacaacta ctatcgggtt cgacttccgg caatctgtcg
     2101 ttttgtccga atctgccgca tctggggtat acaacaattg tactcatcat taaaccttta
     2161 ccgcttaaac aatcgggctg accatctctg tcggcatcca tattgtatgc aatggcgtta
     2221 taatattctc ccagggattg cagggcttgt aactcttgtt cagaaagcag aatctcgttt
     2281 ccaaaagggt cgtaaccggg aatgaccgta tgaccttcga gccggagcat ggaaagatca
     2341 ataagggtgt tttcgccttc gctcaaatta cccaatggca acatatttag cccccgggtg
     2401 gataaatggc caatgtaacg gtaggcagaa tccaaaaaaa gcaaagctat ggcagtgccc
     2461 cattcgggat aaatgctaaa acggccactg tcgatatcga tgaactgatt tccccaataa
     2521 ctaagcacca ggactttgtc ggcgtcttcc aaactcagac tgtcggcaga tctggttaaa
     2581 ccggctttgg ttctttgagg gatttgccct tttataagca ggccctggtt ttgatcctgc
     2641 tcatgttctc tatcattatt atattgacag gcagccaggc agagcaggca ggccagcaat
     2701 gaaatcttgg ttctcatggt tttctttgtt agacaattga ttggtttagt tgcaatttgc
     2761 ttgcaaccaa agctcagtta agcaaacagt ctgtccgaaa aaagatgctc tctgggtata
     2821 aattggatgg gcctgggcat atcgtaaacc gatttgcgga gcaaagcgcc aaaatcttcg
     2881 gtattcccgt accgggactg ttcttcgacg ctgattaatt taccaacacc caaacgggga
     2941 aactgcgtgc ttttgttgaa tacttccctg ggcatacgaa t
//
LOCUS       DAOSGL010000282         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_31437_length_2975_cov_4.876370, whole genome shotgun sequence.
ACCESSION   DAOSGL010000282 DAOSGL010000000
VERSION     DAOSGL010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31437_length_2975_cov_4.876370"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKY68_08105"
     CDS             complement(<1..276)
                     /locus_tag="PKY68_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOH25032.1"
                     /translation="MENRQLIKEVTAKAKHWLTEDYDEQTRAAVQAMLDKEDKTELIE
                     SFYKDLEFGTGGLRGIMGPGSNRVNQYTVGAATQGLANYLNKSFAHLP"
     gene            complement(289..2094)
                     /locus_tag="PKY68_08110"
     CDS             complement(289..2094)
                     /locus_tag="PKY68_08110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH25033.1"
                     /translation="MKRLALLVLSACVAFILNAQKDKADLLEQAVLFVQSHRLQDSIS
                     PSYFDLPLRISPYLSANFGELREDHFHSGLDFKTQGVINKPVYAIAPGFVSRASVRVK
                     GFGKAVYLDHPNGYTSVYGHLESFSPRLDSIVKAAQYAAESYEQNLLFSDEQIPVERG
                     ELIGYSGNTGSSGGPHLHFEIRQTESEDPVDPMFWYKDRIKDTSPPKMLALAIYAIDG
                     QGILLNHGGRLSAKEIIQLGQGGTVAPDSKLPLVWGKIGLGIKAYNYMNGTNNLYGLS
                     RIRLFLDGEEIFQQDIGRFSFEESRYLNALVDYEEWVQNRSWIMKSFVEPFNKLDIYP
                     VKINNGHIDVNQEREYDFTYELYDISGNKAEYRFQLQGKQLPFPQEDTTALFFYPAYK
                     NTYTSKDIYLEFPVASFYNKLAFEYDYTPPKNVKNPGLRNHRLYTGVHKIHHPTVPLH
                     KAAFIRIKINDDILSEKNRYYLVRILSNNKIEYIEATYTEGFMEGEILNFGNYVVLGD
                     SLPPKIFHPQLEKNQNNTLIRILVRDDESGLDTYRCTINAKWVLFEYDYKTNTLTADL
                     RDERIGLLPVGNLLQIYVKDKCGNENYREWMFNAE"
     gene            complement(2097..2450)
                     /locus_tag="PKY68_08115"
     CDS             complement(2097..2450)
                     /locus_tag="PKY68_08115"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOH25034.1"
                     /translation="MLIEVLKSKIHRVRVTEANLDYIGSITVDEDLIEAANLIPNEKV
                     TVLNNNNGERFETYVIKGKRGSGVICLNGAAARRALPGDVVIILSYALMDAEEARTFE
                     PCIIFPDTATNRLIK"
     gene            complement(2440..>2975)
                     /locus_tag="PKY68_08120"
     CDS             complement(2440..>2975)
                     /locus_tag="PKY68_08120"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOH25035.1"
                     /translation="FYFGPLEEVMEGVYRKGHFNGVAQIVSKLFEAVQPGKAYFGQKD
                     FQQLLIVCKMVEQLNMKVQIVSCPIVREESGLAMSSRNELLTAEQKRKAALISKYLFE
                     SLTFVSKKRPAEIKEFVINGINAEPALRLDYFEIVNGTTLQSIQEWTDSACVVGCIAV
                     YCGSVRLIDNIVYKNAD"
BASE COUNT          821 a          697 c          585 g          872 t
ORIGIN      
        1 gggcaagtga gcaaaagatt tattcagata attggccaag ccctgagtgg cggctccaac
       61 tgtgtactga ttcactctgt tgctgccggg tcccattatt ccacgtagcc cacctgtacc
      121 gaattccagg tccttgtaaa aagattcaat taattctgtt ttatcttcct tatcaagcat
      181 tgcttgaact gcagccctgg tttgctcatc gtaatcctct gtcagccaat gcttggcttt
      241 ggctgtgact tcttttatca actgtctgtt ttccatagct tattagtatt attctgcgtt
      301 aaacatccac tccctgtaat tttcattgcc acacttatcc tttacataaa tctgcaaaag
      361 attgcccacc ggcaacaaac caatccgctc gtctcttaaa tcggccgtca aggtattggt
      421 cttgtaatcg tattcaaaca aaacccactt agcgttgata gtacagcggt aagtgtctaa
      481 acccgattca tcgtcacgga caagtatgcg aattaaagta ttattttggt tcttttcaag
      541 ttgaggatga aaaatttttg gaggcagact gtcgcctaat acgacataat tcccaaagtt
      601 caggatttca ccttccataa aaccctctgt atatgttgct tctatatatt ctatcttatt
      661 attggataat attctgacca gatagtagcg attcttttca cttaaaatgt cgtcgttaat
      721 ctttatacgg ataaacgccg ctttatgaag tggaacggtg ggatggtgaa ttttgtgtac
      781 acccgtgtat aaacgatgat ttctgagccc gggattctta acattcttcg gaggtgtata
      841 atcgtactcg aaagccagct tattataaaa cgaagctacc ggaaactcca gatatatgtc
      901 tttggaggta taggtgtttt tgtatgccgg ataaaaaaat aaagccgtgg tgtcttcctg
      961 aggaaaagga agctgtttac cctgcaattg aaaacggtac tcggctttgt tccccgatat
     1021 atcgtacaat tcgtatgtaa aatcgtattc ccgctcttga tttacatcaa tgtgaccgtt
     1081 gttgatctta accggataaa tatcgagctt attgaaaggc tccacaaaag atttcataat
     1141 ccaggaacgg ttttgcaccc actcttcata atcgaccaag gcattcaaat aacggctttc
     1201 ctcgaaagaa aagcggccta tgtcttgctg aaatatttct tcaccatcca ggaataagcg
     1261 aatccggctc aaaccgtata gattgttggt gccgttcata tagttgtagg ctttaatacc
     1321 caagcctatt ttgccccaga ccaagggtaa tttgctgtcc ggagctactg tcccaccctg
     1381 cccgagctgt attatttctt ttgcagacag ccgtccgcca tgatttagca aaattccctg
     1441 cccatcaata gcatagatgg ccaaggccaa cattttcgga ggactggtgt ctttgatgcg
     1501 atccttatac caaaacatgg gatcgaccgg gtcttcggat tccgtctgac gaatttcgaa
     1561 atgcaaatgg ggaccgcccg agctacctgt atttccgcta taccctatca actcaccgcg
     1621 ctcaacgggt atttgttcat cggaaaacaa caaattctgc tcataagact cagccgcata
     1681 ttgggccgct ttaactatag aatccaacct tggagaaaaa gattctaaat gcccataaac
     1741 actggtataa ccattgggat gatccaaata aacagccttc ccgaatcctt ttacacgcac
     1801 gctggcgcgc gaaacgaaac ccggagctat ggcgtatacg ggtttattaa ttacaccctg
     1861 agttttaaaa tccaggccgg aatgaaagtg atcctcgcgt agttcgccga aattggcact
     1921 gagataggga gaaatacgta agggcaaatc gaaataagaa ggagagatac tgtcctgcaa
     1981 cctgtgcgac tgcacaaata aaacagcttg ctccaacaaa tcagccttgt ctttttgcgc
     2041 attaagaata aaagcaacac aagccgacaa cactaataat gcaagccgct tcatcctcac
     2101 ttaatcagtc tgttggtcgc ggtgtcgggg aaaataatac agggttcaaa tgtgcgtgcc
     2161 tcttccgcat ccatcaaagc atacgatagt ataatcacga catcgccggg taaagccctg
     2221 cgagccgcag ctccattaag acaaataacg ccggaacctc ttttcccttt gataacgtaa
     2281 gtttcgaaac gttctccgtt attgttgttc aataccgtaa ccttttcgtt aggaatgagg
     2341 ttggcagcct caatcaggtc ttcgtcgaca gtgatactac cgatgtaatc caggttggct
     2401 tctgttacac gcaccctgtg aatttttgat tttaggacct caatcagcat ttttatatac
     2461 tatgttatct attaaacgca cagacccgca atataccgct atacatccaa ctacgcaggc
     2521 agaatccgtc cattcttgta tgctttgcaa ggtggttccg ttaacaattt caaaataatc
     2581 caggcgaagc gccggttcgg cattgatccc gttaattaca aactccttga tttctgccgg
     2641 ccttttcttc gaaacaaagg taaggctttc aaataaatat ttggatatga gggcagcttt
     2701 tcttttttgc tccgctgtta aaagctcatt gcggctgctc atggctaatc cgctctcttc
     2761 ccgtacaatg ggacaggata ctatttgaac tttcatgttc agttgttcga ccattttgca
     2821 aactatgagc aattgttgaa aatctttttg gccaaaatag gccttaccgg gttgcaccgc
     2881 ttcaaaaagc ttgcttacaa tttgagcgac tccgttgaaa tgcccttttc tgtacacacc
     2941 ctccatcacc tcttcgaggg gaccgaaata aaaaa
//
LOCUS       DAOSGL010000283         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_31560_length_2968_cov_4.194301, whole genome shotgun sequence.
ACCESSION   DAOSGL010000283 DAOSGL010000000
VERSION     DAOSGL010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31560_length_2968_cov_4.194301"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PKY68_08125"
     CDS             <1..868
                     /locus_tag="PKY68_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOH25036.1"
                     /translation="HLTTGRSFNVFDQAEYVGAICAPASASYTRKQLDELTEYVKKPQ
                     IGAKGMVYVRCEADGSFKSSVDKFYTQDDLKTWAEACQAQPGDLILILSGATQATRKA
                     LSELRLEMGRRLGFCDKNLYVPLWVVDFPMFEWDEETARYYAMHHPFTSPKPEDVPLL
                     EKDPSQVRANAYDMVINGVELGGGSIRIHDSRLQADVFKILGFTEEEATQRFGFLMDA
                     FKFGAPPHGGIAFGFDRCVSMFAGLDSIRDCIAFPKNNSGRDVMIDAPSNIDQEQLDE
                     LHLKILTPKNKE"
     gene            865..1647
                     /locus_tag="PKY68_08130"
     CDS             865..1647
                     /locus_tag="PKY68_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOH25037.1"
                     /translation="MKVRDICTLIENFAPLKLQEDYDNAGLILGDPATQVKAILVCLD
                     VSEAVLDEALEKGCNMVVSHHPLIFRGIKSITGKNPQERTLIKAIQNKIAIYAGHTNV
                     DAVMDGVNGKICEKLNLSSTAILVPKENMSNVGAGMTGFLTRALEEKDFLILLKQVFG
                     SKMLRHSALTGREINKVAVCGGSGSELISAAIAEGADAFITADVKYHRFAEVDDKLLL
                     IDAGHYETEQFTKDLFVELISKNIANFAVHSSQRETNPVFYT"
     gene            1677..2468
                     /locus_tag="PKY68_08135"
     CDS             1677..2468
                     /locus_tag="PKY68_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOH25038.1"
                     /translation="MSVDSTKAEPKEISVEEKLKALYELQVVASEIDKIKILRGELPL
                     EVQDLEDIIAGLETRIENFKAEIKTCTDRILGCKIDIENSTALIAKYKAQQDNVRNNR
                     EYDFLSKEIEFQGLEIEHAEKKIREAQFSEKAKNEELNLAQEELNERREDLEVKKSEL
                     DEIISETRSEEETLREKAKTIESYIEPRLLQAFKRIRKNARNGLAVVYVQRDACGGCY
                     NKIPPQRQMDIRMRKKVIVCEYCGRIMIDPELAGVETPAEPAGKK"
     gene            complement(2492..2941)
                     /locus_tag="PKY68_08140"
     CDS             complement(2492..2941)
                     /locus_tag="PKY68_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOH25039.1"
                     /translation="MFALNLPDFKYKLRKKDAKNYIFDPLRRKYVFLSPEEWVRQHFC
                     NYLIVHKNYPSGLIVNECSIQLEGLTKRCDTVIYDAHMQVFMLVEYKAPTVTLNQEVF
                     DQIVRYNWALQAKYLTVSNGMNHYCCRIDYLDNTVNFLEDIPAYNEG"
BASE COUNT          890 a          616 c          699 g          763 t
ORIGIN      
        1 gcaccttacg acggggcgtt ctttcaatgt ttttgatcag gccgaatacg tcggtgctat
       61 ttgtgcaccc gcatcggcct cttatacccg caagcagttg gatgaactga cagagtacgt
      121 caaaaagcca cagattggag ccaaaggcat ggtctatgtt cgttgcgaag cagacggcag
      181 cttcaaatct tcggtggata aattttacac tcaggacgat ctgaaaacct gggccgaagc
      241 ttgccaagct cagcccggag atttaatact gattttgtcg ggagccactc aagccacccg
      301 aaaagctctc agcgaactac gcctcgaaat gggccggcga ctgggatttt gcgacaaaaa
      361 tctatatgtt cctttgtggg tggtcgattt tcccatgttc gaatgggacg aagaaacagc
      421 ccgttattac gccatgcacc atcctttcac ctctcccaag cccgaagatg tacctctgtt
      481 ggaaaaagat ccctcacagg tcagggcaaa tgcctacgat atggtaatca acggggtaga
      541 attgggagga ggatccattc gtatacacga tagccggttg caggccgatg tttttaagat
      601 cctgggcttt actgaagaag aggctactca gcgttttggt ttcctgatgg atgccttcaa
      661 attcggagca cctccccatg gcggtattgc ttttggcttt gaccgttgcg tatctatgtt
      721 tgccggtttg gattccatca gagattgcat tgccttccca aaaaacaatt cgggcaggga
      781 cgtgatgata gatgctccct caaacatcga tcaggaacaa ctggatgaat tgcacttaaa
      841 aatacttact ccaaaaaaca aagaatgaaa gttcgggaca tatgtacttt aattgagaat
      901 tttgcccctc taaaacttca ggaggactac gacaacgctg gtttgatact gggagatcct
      961 gctactcagg tcaaagcgat attggtttgt ttggatgtgt ccgaggcggt attggacgaa
     1021 gctcttgaaa agggatgtaa tatggttgtt tctcaccatc ctcttatttt caggggaata
     1081 aaaagcatca cggggaaaaa tccgcaagag cgtaccttga ttaaagccat acaaaacaag
     1141 atcgccattt atgccgggca caccaatgta gatgctgtta tggacggtgt gaacggaaaa
     1201 atttgcgaga aattgaattt aagctctact gccattttag tccctaaaga aaacatgagc
     1261 aatgtcggtg ccggtatgac cggatttctg actcgggcgc tggaggaaaa ggatttttta
     1321 atactcctaa aacaggtatt cggaagcaaa atgttgagac attccgctct tacaggtcgg
     1381 gaaataaaca aagtggctgt ttgcggaggc tcaggcagcg aacttatttc ggcggccatc
     1441 gccgaagggg cagacgcctt tataactgcc gacgtcaaat accaccggtt tgccgaagta
     1501 gacgataaat tgttgcttat agatgccgga cattacgaaa cggagcaatt tacaaaagac
     1561 ctgtttgtag agttaatttc aaaaaatatt gctaactttg ccgtccattc gtcccaaaga
     1621 gagacgaatc cggtttttta tacttaaaca gctaaatcac aaaattttat ataaatatga
     1681 gcgtcgatag tactaaggct gaacccaaag aaatttctgt agaagaaaag cttaaagcac
     1741 tgtacgaatt acaggttgta gcttccgaaa ttgataaaat caaaattctt cgcggcgagc
     1801 ttccgctcga agtgcaggat ttggaagaca tcattgccgg cctggagacc cgcatcgaaa
     1861 atttcaaggc agagataaaa acatgtaccg acagaatttt gggctgtaag atcgatattg
     1921 aaaattctac ggctctgatc gcaaaataca aagcccagca agacaatgtc cgtaacaacc
     1981 gggaatatga ttttctgagc aaggaaattg aatttcaggg tctggaaatc gaacatgccg
     2041 agaagaaaat ccgggaagct caattcagcg aaaaagctaa aaacgaagaa ttgaatctgg
     2101 ctcaggaaga attgaacgaa agacgcgagg acttggaagt gaagaaatcc gaactggatg
     2161 aaatcatttc agaaacccgc tctgaagaag agacactcag ggaaaaagcc aaaaccatcg
     2221 aaagttacat agagcccagg cttctgcaag ccttcaaacg tatacgcaaa aacgcaagaa
     2281 acggtttagc cgtagtttac gtacagcgcg atgcctgtgg tggttgctac aataaaattc
     2341 cgccccaaag gcagatggat atcagaatgc gcaaaaaagt aattgtttgc gaatactgtg
     2401 gccgcattat gatcgatccc gaattggcag gagtggaaac accggcagaa ccggccggta
     2461 agaaataatt tttatccggt ttttgcagga atcaaccctc gttgtaagcg gggatatctt
     2521 ccaaaaaatt cactgtattg tcaaggtaat ctatcctgca gcaataatgg ttcataccgt
     2581 tactgactgt caggtatttg gcttgtaaag cccagttgta acgtacaatt tggtcaaaaa
     2641 cttcctgatt gagcgttacc gtgggagctt tgtattctac cagcataaat acttgcatat
     2701 gagcatcgta tataacggtg tcgcaacgtt tggttaagcc ttccagttgg atgctgcact
     2761 cgttgacgat aagtccggac ggataatttt tatggacaat caggtaattg caaaaatgct
     2821 gccgcaccca ttcttcggga ctgagaaaaa cgtatttacg cctgaggggg tcgaatatgt
     2881 agttcttagc atctttcttg cgcaatttgt acttgaagtc gggcaggttt aatgcaaaca
     2941 tttcgtattt ttggaatcaa tttgtatt
//
LOCUS       DAOSGL010000284         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_31685_length_2960_cov_3.546988, whole genome shotgun sequence.
ACCESSION   DAOSGL010000284 DAOSGL010000000
VERSION     DAOSGL010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31685_length_2960_cov_3.546988"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2402
                     /locus_tag="PKY68_08145"
     CDS             <1..2402
                     /locus_tag="PKY68_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOH25040.1"
                     /translation="GATVTVRQEADIENPVLWSNENPYLYKLVTSLKQGRKKKDALET
                     PFGIRTISWPVKRNDGDGRFFLNGKPVFLNGTCEYEHLLGQGHAFSREQVKARVKQML
                     NAGFNAFRDAHQPHHLDYQKYWDKYGILFWTQLSAHIWYDTPVFRENFKTQLRQWVKE
                     RRNSPSVVLWGLQNESMLPKDFAVECTAIIREMDPTALNMRAVTTCNGGVGTDWNVVQ
                     NWSGTYSGNPERYDEELALPNQLLNGEYGAWRTLDLHEAPGPFKQEGAYSEERFCQLM
                     EMKIHLAEKARDKVCGQFQWIFNSHDNPGRRQPDEAYRLIDRVGPVNYKGLITPWEEP
                     LDAYYMYRSNYVPADTDPMVYIASHTWPDRFEEPCRACIQVYSNCDSVQLYNDAGNRA
                     YLGTEVHQKVKGTHFAFENCLVQYNVLRAVAYRSGKAVAEDLIVLNNLKKAPSFDLLY
                     KKDKSLTKGKSSYNYIYRVNCGGDDYIDSFGQLWAKDDTSFSRSWAQDFAGLNPYQAS
                     QRVTNDPIRGSRDWPLFGHFRYGRHKLAYRFPLPDGKYRLELYFIEPWHGTGSGKGGD
                     CAGLRLFDVSANGKILLNDLDIWAESGHDVALKKTFRVKVQGGFLNLEFPEIKAGQAV
                     LSAIAIASRDKSIKPHVPPVSDWSWQKADRERVGRFPTERLPEDNNVRPGVSYPAVEA
                     VVEGNYRKEQMNNRMAVIFEAASTNSISWDITTGLAQLYALRFNYRNISGRDMLVDMK
                     IIAADGTVLKEDKLTFPINERGWKLISTTTGSFINAGHYRIVLSSPDMDGLCFERLDV
                     Q"
     gene            complement(2396..2905)
                     /locus_tag="PKY68_08150"
     CDS             complement(2396..2905)
                     /locus_tag="PKY68_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25041.1"
                     /translation="MRSGFIIALCFLFSSVAFSQRPGGEANLPGRVPNQEERQQQWEE
                     MRLKRIAYYTDKIGLTAEEAQLFWPIVNDIQNRSLELNHEMMRLMRVSRQGRENQKID
                     YEKINARMAEIRQQRADLEKERYVKLKAILSPEKLFKYYLAEEGFSRELLRSLNTGRQ
                     ANSKDILPY"
BASE COUNT          745 a          684 c          761 g          770 t
ORIGIN      
        1 cgggagcgac agttactgta cgccaggagg ctgacataga aaatccggtg ctgtggagca
       61 acgaaaatcc ttatttgtat aaattggtta ccagtttaaa gcaaggaaga aagaaaaaag
      121 atgcattgga aacccccttc ggtattcgca ccatcagctg gccggtgaag cgcaacgatg
      181 gcgacgggcg tttctttttg aacggtaagc cggtgttttt gaacggcact tgcgagtatg
      241 aacatctttt ggggcaaggc catgctttta gtcgtgaaca ggtgaaggct cgtgtaaagc
      301 aaatgctcaa cgccggtttc aatgctttca gagacgccca tcagccccat cacctcgatt
      361 atcagaaata ttgggataaa tacggaatct tattttggac acagctatcg gcgcatatct
      421 ggtacgatac ccccgtattc agagaaaatt tcaaaactca gctcaggcaa tgggtaaaag
      481 agcgccgaaa cagcccttcg gtggtgcttt ggggactgca aaacgaaagc atgttgccca
      541 aagactttgc cgtagaatgc accgccatca ttcgtgagat ggaccccacg gcgctgaata
      601 tgcgtgcggt taccacctgc aacggcgggg taggcaccga ctggaacgtg gtgcaaaact
      661 ggagcggcac ctacagcggc aatccggaac gttacgacga agaactggct ttgcccaacc
      721 agttgctcaa cggtgaatac ggagcctgga gaaccctgga tctgcacgaa gcgccgggac
      781 cctttaagca ggaaggcgcc tattcggaag aacgtttttg tcaattgatg gagatgaaaa
      841 tccatctggc cgaaaaagcc cgcgacaaag tctgtggcca gtttcaatgg atattcaaca
      901 gccacgacaa tcccggacgc cgtcagcccg acgaagccta cagattaata gaccgggtgg
      961 ggccggtcaa ttacaagggg cttattactc cctgggaaga acctttggat gcttattaca
     1021 tgtatcgttc caattatgtg ccggccgata cggatcccat ggtctatata gcttcgcata
     1081 cctggcccga tcgttttgag gagccctgca gggcatgcat acaagtatac agtaattgcg
     1141 attcggtgca attgtacaac gacgccggta accgggctta tctgggaaca gaggttcatc
     1201 agaaagtaaa aggtacccat tttgcctttg aaaattgctt ggtgcaatac aatgtgttgc
     1261 gagctgttgc ttatcgttcg ggtaaggctg tggcggagga tttgatcgta ctgaacaacc
     1321 taaaaaaagc accctctttc gatctcttgt acaaaaaaga caaatccctg accaaaggca
     1381 aatcttcgta taattacatt tatcgcgtca attgcggagg agacgactac atagacagtt
     1441 ttggccagct ttgggccaag gacgatacca gtttctcccg ttcctgggct caggattttg
     1501 ccggtctcaa tccctatcag gccagtcagc gtgtcaccaa cgatcccatt cgcggaagcc
     1561 gggattggcc tctgttcggt catttccgtt acggcaggca taaactggct taccgttttc
     1621 ctctgccgga cggtaagtac aggctggagt tgtattttat cgaaccctgg cacggcacag
     1681 gatcgggtaa gggcggcgat tgcgccggtc tgcgcctttt tgatgtttcc gcaaacggaa
     1741 aaattctctt gaacgacctg gatatatggg ccgaatcggg tcacgatgtc gctttgaaaa
     1801 agactttccg ggtgaaagta caaggcggtt ttctaaacct tgaatttccc gagataaaag
     1861 caggtcaggc cgttttgtcg gccattgcta ttgccagccg cgacaaaagc attaagcctc
     1921 atgtgcctcc ggtttcggac tggagctggc aaaaggcaga ccgggagcgg gtggggcgtt
     1981 ttccaaccga gcgattgccc gaagacaaca acgtcagacc gggtgtgtct tatcccgccg
     2041 tagaggccgt tgtcgaaggc aattacagga aagaacagat gaacaacagg atggctgtga
     2101 tttttgaagc agcaagcaca aacagcataa gttgggatat tacaaccgga ctggctcagc
     2161 tctacgcttt gcgttttaat taccggaata tctccggccg cgatatgctg gtggatatga
     2221 agataatagc tgcagacggc accgtgctca aagaagataa actgactttt cccatcaatg
     2281 agaggggctg gaagctgatc agcaccacta ccggttcgtt catcaacgcc ggtcattacc
     2341 ggatcgttct ttcttcgccc gatatggatg gtctgtgttt cgaaagactc gatgttcagt
     2401 aaggcagtat atctttgctg ttggcttgtc tgcctgtatt taaagatctg agcagttctc
     2461 tggaaaagcc ttcttcggcc aaataatatt tgaaaagttt ttcggggctg agtatggctt
     2521 tgagtttaac gtagcgctct ttttccagat cggcccgttg ttggcggatt tcggccattc
     2581 ttgcattgat tttttcgtaa tcgattttct ggttctctct cccttgtctg gagacgcgca
     2641 tcaaacgcat catttcgtgg tttagttcaa gacttctgtt ttggatgtcg tttacgatag
     2701 gccagaacaa ctgagcttct tcggcagtaa gcccgatttt gtctgtataa taggcaattc
     2761 ttttgagacg catttcttcc cattgttgtt gcctttcttc ctgattgggt accctgccgg
     2821 gtaggtttgc ttcaccgccg ggcctttgtg aaaaagcaac gctgctgaat aaaaagcata
     2881 aagcgattat aaagccggat ctcattttta gttggttttt ggactgaatt tattcgagtt
     2941 cgtcctgatt aatgtaataa
//
LOCUS       DAOSGL010000285         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_32010_length_2940_cov_3.620104, whole genome shotgun sequence.
ACCESSION   DAOSGL010000285 DAOSGL010000000
VERSION     DAOSGL010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32010_length_2940_cov_3.620104"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PKY68_08155"
     CDS             <1..672
                     /locus_tag="PKY68_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOH25042.1"
                     /translation="FRFPTVVKKVTPGSPGDKAGLMPGDSIVGLNSVQTESFTDFASM
                     LSQYGDSSILLSYYRSGNPGTMQVQLDSTAKIGFELKHPADIYPLKQINYSFFASIPA
                     GIKLGVSKLTGYAGDMKYVFTREGAKSLGGFGAIGSLFPATWNWKIFWETTAFLSIIL
                     AFMNILPIPGLDGGHILFLLFEVISRRKPNEKFLEYAQMAGMFILLALLIYANGNDLF
                     RWLFK"
     gene            761..1786
                     /locus_tag="PKY68_08160"
     CDS             761..1786
                     /locus_tag="PKY68_08160"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOH25043.1"
                     /translation="MKYLTVCCLLLTAVFSWAERQLRVVFYNTENFFDCKDDSLTRDE
                     DFLPGGSYAWSPKRFLQKTEQLARVLAVAGEESFPHLVGLAEVENEDCLQFLLRASSL
                     KNAAYRYIYAESADPRGIDVCLLYNRFVFKPLAWESIRPSFSDENKKSRDILYVNGLL
                     PNEQVLHVFVCHLPSRYGGVKASEKFRRQAAGQLRARVDSLFSKEPNALILIMGDFND
                     EPCDLSLSRDLGARNPQEPYQPAALYNMMWPLMNKPAYGSHKYQGRWLMIDQIIVSSA
                     LLYEEPLQQESPPIRRLSGAKVRFDDFLLVKDEKYLGYKPFRTYEAYRYIGGYSDHLP
                     VCLDIPF"
     gene            1917..2657
                     /locus_tag="PKY68_08165"
     CDS             1917..2657
                     /locus_tag="PKY68_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH25044.1"
                     /translation="MISVCMATFNGERYLKEQLDSILSQLGDEDELIVSDDGSSDKTQ
                     EIIHAYEDKRIRFFHNQRKGIANNFENALSQARGDFIFLADQDDVWLAGKLDKMVEFL
                     EKESLDVAMCNCALVDQELNIIKSPHFDSEWPMKRTLLSNLYKNAWLGCCMVLRSSVL
                     KATMPFPKGLVAHDLWLALYAQWHFRTGYMDEVLVLYRRHDNTVSFTGSVSTNALSFK
                     LKYRLHLLYHLIGRSLKNSFSGPVIKYG"
     gene            2660..>2940
                     /locus_tag="PKY68_08170"
     CDS             2660..>2940
                     /locus_tag="PKY68_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOH25045.1"
                     /translation="MKVLFLNPPFHDHFSRESRSPAVTKSSTIYYPKWLSQAAGVAIK
                     EGHEVDLIDAPAWCVDVQYVMDRIVAKNIEAMVVDCSTPSIINDIQVVDR"
BASE COUNT          772 a          628 c          700 g          840 t
ORIGIN      
        1 ttccgttttc cgacagtggt caaaaaagta acgcccgggt ctccgggaga taaagccggg
       61 ctcatgccgg gcgacagtat tgtaggcctt aactcggtgc agacggaatc ttttacggac
      121 tttgcttcta tgctcagtca atacggagac agcagcattt tgttgagtta ttaccgctcg
      181 ggaaacccgg gcaccatgca agtacagctg gattccactg ccaaaatcgg ttttgaactt
      241 aaacatccgg ccgatattta tcccttgaaa caaataaact attccttttt tgcctccata
      301 ccggcgggta taaaattagg cgtaagcaaa ctgaccggtt atgccggcga tatgaaatac
      361 gtatttacca gggaaggagc caagagcctg ggcggatttg gtgccatagg aagtttgttc
      421 cccgccacct ggaattggaa aatcttctgg gaaaccacgg cctttttgtc tattattctg
      481 gcttttatga atattttgcc tattccggga ttggacggcg gccatattct ctttttattg
      541 ttcgaggtga ttagtaggcg caaacccaac gaaaaatttt tggaatacgc ccagatggcc
      601 ggtatgttta ttttacttgc cttattaatt tacgctaacg gcaacgactt gtttcgttgg
      661 ctgtttaagt aaaactatac taatctaaaa aaatcattat ttttttgata aaagaatgct
      721 atatttgcac aaaacaaaat ttgtgcaaat acggtatctt ttgaaatatc ttacggtttg
      781 ctgcctgttg ctgacggccg tgttttcatg ggcggaaagg cagcttcggg tggtgtttta
      841 caatacggag aatttctttg attgcaaaga cgacagcctg acacgtgatg aggatttttt
      901 gcccgggggg tcttacgcct ggagtcctaa acgttttctg caaaaaacgg aacagctggc
      961 cagggtgctg gctgtggcag gcgaggagag tttccctcat ttagtgggcc tggcagaggt
     1021 ggaaaacgaa gactgtctgc aatttttact cagggcctct tccctgaaaa acgccgctta
     1081 ccgctatatt tacgcggaat cggccgatcc taggggaata gatgtttgtc tgctgtacaa
     1141 tcgctttgtt ttcaaacctt tggcctggga aagtataagg ccctcgttca gcgacgaaaa
     1201 taaaaaatca agagacatcc tctacgttaa tggattactg ccaaatgaac aagtactgca
     1261 tgtatttgtt tgtcatctgc cttcgcgtta cggcggggtc aaggcttccg agaaattcag
     1321 acgtcaggca gccgggcaat tgcgcgcaag agttgactcc ctgtttagca aagagcccaa
     1381 tgcacttatc ctgattatgg gcgattttaa cgatgagccc tgtgatttgt ctttgagtcg
     1441 tgacttggga gcgaggaacc ctcaggagcc ttaccagcct gcggccttgt acaatatgat
     1501 gtggcccctg atgaataaac ctgcttacgg tagtcacaaa taccaggggc gctggttgat
     1561 gattgaccag atcattgttt cttcggcttt gttgtacgaa gagcccctgc aacaagagag
     1621 cccgcctatt cgccgtttgt ccggggcaaa ggtaagattt gatgattttt tgctggtcaa
     1681 agatgaaaag tatttgggtt ataaaccctt tagaacctat gaagcatatc gttacatagg
     1741 cggttacagc gatcaccttc cggtctgcct ggacattccg ttctgagctt taaaaattcc
     1801 cttttgatag tccgtacaat acgaaaatta gctatctttg tctgccaaac aaaactcata
     1861 ttgatactgg ttttcaatct gagaaagttt aaccacctac caatttataa agggcaatga
     1921 tatcggtatg tatggctacc tttaacgggg aacggtattt gaaagaacag ttggattcca
     1981 ttctgagcca gcttggcgac gaggacgaac taattgtttc tgacgacggc tccagcgata
     2041 aaactcagga aatcatccat gcatacgaag ataaaagaat ccgttttttc cacaaccaac
     2101 gcaaaggcat cgccaacaat ttcgagaatg ctttatccca ggcccggggt gattttattt
     2161 ttttggcaga ccaagacgat gtctggttgg ccggtaaatt ggataaaatg gtggaattcc
     2221 ttgaaaagga aagcctggat gtggctatgt gcaattgtgc tttggtagat caggagctta
     2281 acattattaa atcaccgcat tttgattcgg aatggcccat gaagcgaaca cttttgtcca
     2341 atttatacaa aaatgcctgg ctgggttgct gcatggtttt acgttcctcg gtattaaaag
     2401 cgaccatgcc ctttcctaag ggcctggtgg ctcacgacct atggttagcc ttgtatgctc
     2461 aatggcactt caggaccggt tatatggacg aagtattggt gttgtatcgc aggcacgaca
     2521 atacggtttc gtttacaggc tccgtcagca ccaacgcact atcgttcaag ctgaaatacc
     2581 gcttgcattt attgtatcat cttataggca ggagcctgaa aaactcgttt tccggccctg
     2641 tgattaaata cggatagtaa tgaaggtatt gtttttaaat cctccttttc acgaccattt
     2701 ttccagagaa tccagaagtc ctgcggttac aaaaagtagt actatttatt acccgaaatg
     2761 gttgtctcag gctgcaggtg tagccatcaa agagggacat gaggtcgatt tgatagatgc
     2821 ccccgcctgg tgtgtcgacg tgcaatacgt catggacaga attgtcgcca aaaacattga
     2881 ggccatggtg gttgattgct ctaccccgag tatcataaac gacatacagg tggttgaccg
//
LOCUS       DAOSGL010000286         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_32194_length_2928_cov_4.257222, whole genome shotgun sequence.
ACCESSION   DAOSGL010000286 DAOSGL010000000
VERSION     DAOSGL010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32194_length_2928_cov_4.257222"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="PKY68_08175"
     CDS             <1..1171
                     /locus_tag="PKY68_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH25046.1"
                     /translation="REGEKKLIKKTLQRKLIQMNPVEYAGLLRPGVGYVYLSSFTDKS
                     YQELRQTLINLNKQGAQSLILDLRDNGGGLLSEAVSICNLFVDKGEVIVTTKGKAPQW
                     DKTYRTLMEPLFEDMPVVVLTNTLSASSSEIVAGALQDLDRAVILGSRTFGKGLVQTT
                     RELAYNGVLKVTTAKYYIPSGRCIQAVNYANGRSGRLPDSLTTQFSTRNGRPVRDGGG
                     ISPDVSCVQKEYSDFCWQLVNNLYFFDFANDYYRKHPKITSVENFKLSEQDFNDFKNH
                     IRNSDFRYELHSKSKLNELKKTLESEGYWEDVSDDFKSLESKLVHDLDKDLETFRPDI
                     ERILSLEIIRRYYYQKGEIQESLKTDECVSKALDLLMNRESYKAILQPSKQQAAL"
     gene            1358..1429
                     /locus_tag="PKY68_08180"
     tRNA            1358..1429
                     /locus_tag="PKY68_08180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Glu,seq:ttc)
     gene            1453..1704
                     /gene="rpsT"
                     /locus_tag="PKY68_08185"
     CDS             1453..1704
                     /gene="rpsT"
                     /locus_tag="PKY68_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOH25047.1"
                     /translation="MANHKSALKRIRQAQARRLRNRYYAKTTRNAVRDLRATTDKAEA
                     EKMFPGVVSMLDRLAQKGIIHKNKAANLKSKLAKSVAKL"
     gene            1877..2623
                     /gene="fabG"
                     /locus_tag="PKY68_08190"
     CDS             1877..2623
                     /gene="fabG"
                     /locus_tag="PKY68_08190"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOH25048.1"
                     /translation="MKLLEGKTALITGAARGIGKAVALRFAQEGANIAFTDLVLNELV
                     LETEAEIAAYGVKVKAYASDASNFEETHRLVDDIVKEFGKVDILVNNAGITKDGLMMR
                     MSEAQWDAVININLKSAFNFIHALTPVMMKQRGGSIINMSSVVGVSGNAGQANYSASK
                     AGMIGLAKSVAAELGSRGIRANAVAPGFIITEMTGQLPEDVRKAWCEGIPLRRGGSPE
                     EVANVCVFLGSELSSYVSGQVIHCCGGMNR"
     gene            2631..>2928
                     /locus_tag="PKY68_08195"
     CDS             2631..>2928
                     /locus_tag="PKY68_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOH25049.1"
                     /translation="MTILYEDNHLIIVNKSCSEIVQGDKTGDPSLEEEIKQWIKQTYH
                     KPGNVFLGVTHRLDRPVTGVVIFAKTGKALARINEMFRKGEIQKTYFALVKNSPP"
BASE COUNT          858 a          746 c          675 g          649 t
ORIGIN      
        1 acgcgagggc gaaaagaagc tgataaaaaa aacgctgcaa cgtaaactca tacagatgaa
       61 tccagtagaa tatgccggct tgctgagacc gggcgtgggc tacgtatatc tgagcagctt
      121 caccgacaaa tcctatcagg aactcagaca aaccttgatc aatctcaaca aacaaggcgc
      181 tcaaagtctc atcctcgatt tacgcgacaa cggagggggc ttactaagcg aagcggtttc
      241 catctgcaac ttgtttgtag acaaaggtga agtcatcgtc accaccaaag gcaaagcgcc
      301 tcaatgggac aagacctaca gaacgcttat ggagccgctt ttcgaggata tgccggtggt
      361 cgtgcttaca aatacgcttt cggcttcttc ttcggaaatt gtggccggag ctttgcaaga
      421 tttagacagg gccgttattt tgggcagccg tacgtttggc aagggcctgg tacaaaccac
      481 cagagaattg gcctataacg gagtgctcaa agtgaccacc gccaagtatt acatccccag
      541 tggacgttgc atccaggccg tcaattacgc caatggccgc agcggaaggc tgcccgacag
      601 cctgaccacg caattctcca ctcgcaacgg tcgcccggtc agagacggag gtggcatcag
      661 tcccgatgtg agttgcgtcc aaaaagaata ttccgacttt tgttggcaac tggtaaacaa
      721 tctgtacttt ttcgattttg ccaacgacta ctaccgcaaa caccccaaaa taacttcggt
      781 cgaaaacttc aagctcagcg agcaggattt caacgacttc aaaaaccaca tacgcaacag
      841 cgatttcagg tatgaattac atagtaagag caaattgaac gaactcaaaa aaacgttgga
      901 atccgaaggt tattgggaag acgtcagcga cgacttcaaa tcgctcgagt ccaagctggt
      961 ccacgatctg gacaaagacc tcgaaacctt cagacccgac attgagcgca tcttgagcct
     1021 cgaaatcatc aggcgttact attatcaaaa aggagaaatt caggaatcgc tcaaaacgga
     1081 tgaatgcgtg tcaaaagccc tcgatttgct gatgaacaga gaaagctaca aagcaattct
     1141 ccaaccttcg aaacagcaag cagctctttg acggcacctg cgctcccaaa cgcgtaataa
     1201 caacccgacg caaaaccgat aatcggtcga tttcaaaatt atatgcgctc cgataaaatt
     1261 tttcctgaaa aacttttgcc ggaaaataaa atcgtcttat ctttgcagcc gcaaaattag
     1321 cgccggcaat ggccgaaccg cttttttgcc acaggctggc ccattcgtct atcggttagg
     1381 acgcaagatt ttcattcttg aaagagcggt tcgattccgc tatgggctac aaaaacacta
     1441 aagaaaaaaa caatggcaaa ccacaaatca gccctcaaaa gaatacgcca ggcacaggcc
     1501 agacgcctgc gcaaccgtta ctacgccaaa accacccgta acgctgttcg cgacctgcgt
     1561 gccaccaccg acaaagccga agcggaaaaa atgtttcccg gcgttgtgtc catgttggat
     1621 cgtttggccc aaaaaggaat tatccacaag aacaaagctg ccaatttaaa atctaagctg
     1681 gcaaaaagcg tggccaagct ctaaaaagct tctccacttg ccgtcttcat aagtcagacc
     1741 acagatttcc tgtggtcttt ttttgtgctc tccgcttgcc aaaccacaag aaaatcttac
     1801 ttttgcaaaa ttctcagcta ggacgtaagc tttacattta cggcttgcct cctaccctta
     1861 accaaataat acgattatga aattattaga aggaaaaacc gctttaataa ccggcgctgc
     1921 cagaggaata ggcaaagcag tcgcattgcg ttttgcacaa gaaggcgcca acattgcttt
     1981 taccgatctg gttttgaatg aactagttct cgaaaccgaa gccgaaatag cagcctacgg
     2041 agtcaaagtg aaagcctacg cctccgacgc ttccaatttt gaagaaaccc accggctggt
     2101 cgacgacata gtaaaggaat ttgggaaagt agatatcctg gtcaacaacg ccggtatcac
     2161 caaagacggc ttaatgatgc gcatgagcga agcccaatgg gacgccgtca tcaatatcaa
     2221 cctgaaatcg gcctttaatt ttatccatgc ccttaccccg gtaatgatga aacaacgcgg
     2281 aggcagcatt atcaatatga gttccgtggt gggtgtatcg ggcaacgccg ggcaagccaa
     2341 ctattcggcc tccaaagccg gcatgatcgg cctggccaaa tcggtggctg ccgagcttgg
     2401 ttccaggggc atacgcgcca atgcggttgc tcccggcttt attatcaccg aaatgacagg
     2461 ccaattaccc gaagacgtac gcaaagcctg gtgcgaaggt attccgcttc gccgcggggg
     2521 cagccccgaa gaggtggcca atgtttgtgt tttcctgggc tcagagcttt cttcgtatgt
     2581 aagcggacag gtaatccatt gttgcggggg aatgaaccgc taaacgcttt atgactatcc
     2641 tctacgaaga caatcacctg attattgtca ataagtcttg ttctgaaatt gttcagggcg
     2701 acaaaacagg cgacccctcg ctggaggaag agatcaagca atggattaag caaacctacc
     2761 acaagcccgg caatgtcttt ttgggcgtga cccatagatt ggatcggccg gttacggggg
     2821 ttgtgatctt tgccaaaacc ggcaaagcgc tggcccgtat aaacgaaatg tttcgcaagg
     2881 gggaaattca gaagacttat tttgccctgg tcaaaaacag ccctcccg
//
LOCUS       DAOSGL010000287         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_32454_length_2913_cov_3.730931, whole genome shotgun sequence.
ACCESSION   DAOSGL010000287 DAOSGL010000000
VERSION     DAOSGL010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32454_length_2913_cov_3.730931"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKY68_08200"
     CDS             complement(<1..808)
                     /locus_tag="PKY68_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOH25050.1"
                     /translation="MILYLLLVVAGLICLIYGANWLVDGASALAKKYKMSDLAIGLTI
                     VAFGTSAPELVVNVVASINGHSDLTLGNVIGSNNFNTFIILGLSGLIMPIAVQSSTAH
                     KEIPMSLFITVLLLFMLNDFRFSGEGSLSRFDAIVLFALFLLFMYYVFNQMKGDSAPA
                     TAVVVKSNVTIGSLILLGLVGLAAGGHLVVKNSVAIANALGVSEKIIGLTIVAAGTSL
                     PELATSVVAAIKKNSDIAIGNIIGSNIFNISLILSVSAFIRPIAYNPKFNQ"
     gene            complement(876..1910)
                     /locus_tag="PKY68_08205"
     CDS             complement(876..1910)
                     /locus_tag="PKY68_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOH25051.1"
                     /translation="MRNFLLIALMLGLASCGLQKNKPLTEKDMSAYLLVYFLDNTHSL
                     HMALSEDGYTFTAVNEGKAVVAGDTIAGQKGIRDPHITRGPDGAFYLAMTDLHIFGRQ
                     RGYRDTQWERPQEEYDWGNNRGFVLMKSFDLIHWTHNKVYIQEAFPHLDVGCAWAPQC
                     IYDPQEEKMMLYFTMRLGHGKTQLYYAYTDDDFTRLLTEPQLLFEYPNPAVQILDADI
                     TSMPDGRYCMMYVAQENPGGIKMAVSDKINSGYVYDERWVDLEPGACEAPNVWKRIGQ
                     DKWVLMYDIFSIRPHNFGFVETSDFEHFESLGRFNEADMKTTNFSSPKHGAVIQLTKK
                     EAKTLSDYWK"
     gene            complement(2034..>2913)
                     /locus_tag="PKY68_08210"
     CDS             complement(2034..>2913)
                     /locus_tag="PKY68_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH25052.1"
                     /translation="WGAGTWEIGYRDEDFTFQGNIETGVIKKVGAGIMTLSSAMTSTA
                     NFLINEGGLTVTGLTSGPGTSNVYIKYGAFMSGNGNIQGSIIVEEGGSLYAGLYQPNN
                     LTAGTSFRTANVQMQTGSHFVVKVDPGMEKSDMMVTSGNFVARGNLIMQNISGQPYRE
                     GMSFQIVRPGTITGGFASVEPESPGEGLEWDLNDFTSTGRINVVAASALDKARAAELL
                     VYPNPTAGKLFVQLPRAAGEWRIKLENLDGQLLMEQSACGSRLLEIDLTHLKQGLYII
                     TVYSGEQWMKRKVLLK"
BASE COUNT          766 a          733 c          655 g          759 t
ORIGIN      
        1 cctgattgaa ttttggatta tatgcaatcg gcctgataaa ggcacttaca gataaaatca
       61 acgatatatt gaagatgttg gagccaataa tatttccaat ggcgatatcg gaattctttt
      121 ttatggcagc caccaccgag gtcgccaatt ccggcaaaga agttccggcg gccactatgg
      181 tcaatccgat gattttttca ctgacaccta aagcgttcgc aatcgcgaca ctattcttta
      241 caaccaaatg cccccctgct gccagcccaa ctaatccaag cagtatcaaa ctccctattg
      301 ttacgttcga ttttaccact acggcggtag ccggagctga atcgcctttc atttggttaa
      361 acacataata catgaagagc aaaaacagcg cgaataaaac aatggcatca aaacggctta
      421 aagatccctc gcctgaaaac ctgaaatcgt tcagcataaa caagagtaaa accgtgataa
      481 acaaagacat gggaatttct ttgtgtgcag tagaactctg taccgcaatg ggcataatca
      541 ggcccgacaa accgagaatg atgaaagtat tgaaattatt acttcctatt acattcccca
      601 aagtaaggtc ggaatggccg tttatggaag cgaccacgtt gaccaccaat tcaggtgccg
      661 aagtgccgaa agccacaatg gtgagcccaa tggccaaatc ggacatttta tatttcttcg
      721 ctaaagcaga ggcaccgtcg accaaccaat tcgcaccgta aattaaacaa ataagaccgg
      781 caacaacgag cagtaagtat aggatcatat agggttcatt attttatctg taccgttcaa
      841 aacaggcggt gcagcttggg gcattcatgt gtgaatcatt tccagtaatc actcagtgtt
      901 tttgcttctt ttttggtcag ttgaataaca gccccgtgtt tgggagagct gaagttggtc
      961 gttttcatat cggcttcgtt aaaacggccc aaggactcaa aatgctcaaa atcggaggtt
     1021 tccacgaaac cgaaattatg gggccggatg ctgaaaatat cgtacatcaa cacccatttg
     1081 tcttggccta tccttttcca cacattcgga gcttcgcaag cgccgggttc aagatcgacc
     1141 cagcgttcat cgtacacata gccgctgttg atcttgtcgg aaacggccat tttaatgccc
     1201 ccgggatttt cctgagccac gtacatcatg cagtaacggc cgtcgggcat ggatgtaata
     1261 tcggcatcca aaatctgaac agccggattg ggatattcaa aaagcaattg aggctccgtg
     1321 agcaggcggg taaaatcatc gtcggtataa gcgtaataca attgggtctt gccgtgtccc
     1381 agccgcatgg taaagtaaag catcatcttt tcctcctgcg gatcgtaaat gcattgcgga
     1441 gcccaggcac agcccacatc caaatgggga aaggcttcct gaatgtacac cttgttgtgt
     1501 gtccaatgga taagatcaaa ggatttcatc aatacaaaac cccggttgtt gccccagtcg
     1561 tattcttcct gaggcctctc ccactgggtg tcgcgataac cccgctgtct gccgaaaata
     1621 tgcaaatcgg tcatagccag gtaaaaagca ccgtccgggc cacgggtaat atgcggatcg
     1681 cgtataccct tctgaccggc tatggtgtcg ccggctacca cggctttacc ctcgttgacg
     1741 gctgtaaagg tatatccgtc ttcgctcagg gccatgtgca gactgtgggt attgtccaga
     1801 aaatacacca gcagataggc agacatgtcc ttttcagtca aaggcttatt cttttgtaaa
     1861 ccacaggaag ccaaacccag catcagggca atcaataaaa aatttctcat tgctttatgt
     1921 tttaaaattt ccaactaata atcggttttt ctgtttttcg gttttttacc agagcttctc
     1981 aagtgcttct caagtacttg atttacactc ggcggtatgg tttcgatgta tcattatttc
     2041 aacaatactt tccgtttcat ccactgctca ccggaatata ccgtgatgat ataaaggcct
     2101 tgtttcaaat gagtcaggtc tatttcgagc agtctgctgc cgcaggcgct ttgttccatc
     2161 aggagctgtc cgtcgagatt ttccagttta atacgccatt cgccggcggc ccggggcagt
     2221 tgaacaaaaa gcttccctgc tgtcgggttg ggataaacca ggagctcggc ggcccgggcc
     2281 ttgtcgagag ctgaggcggc caccacgttg atacggccgg tcgaggtaaa atcgttcaaa
     2341 tcccattcca gaccttcgcc cgggctctcc ggctcaacgg atgcaaagcc tcccgtaatg
     2401 gtgcccggcc tcactatctg gaaagacatg ccctcccgat aaggctgacc tgaaatgttt
     2461 tgcatgatta agttgcctct ggcgacaaaa ttgcccgaag taaccatcat atccgatttt
     2521 tccattcccg ggtctacttt gaccacgaaa tgggaaccgg tctgcatttg cacgttggcg
     2581 gttctgaaag aggtgcccgc cgtcaggttg ttgggttgat acaagccggc gtacaggctg
     2641 ccgccttcct ccactattat gcttccctga atatttccgt tgccgctcat aaaagcgccg
     2701 tattttatat aaacattcga ggtaccgggg ccgctggtaa gacccgtaac agtgagccct
     2761 ccctcgttga tcaggaaatt ggccgtagaa gtcatggcag aactcagggt cataatacct
     2821 gcacctactt tcttgatgac accggtttcg atatttccct gaaaagtgaa gtcttcgtcc
     2881 cggtaaccga tttcccaggt acctgctccc cat
//
LOCUS       DAOSGL010000288         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_32519_length_2908_cov_4.124781, whole genome shotgun sequence.
ACCESSION   DAOSGL010000288 DAOSGL010000000
VERSION     DAOSGL010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32519_length_2908_cov_4.124781"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKY68_08215"
     CDS             complement(<1..436)
                     /locus_tag="PKY68_08215"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOH25053.1"
                     /translation="MKHRQIILPPEWAKQAFVQLTWPHKDSDWADNLEEVEVCYIEIA
                     RHILRYEPLLVVCSHPNSVRAKIAPQSGQKLFLYELPCNDTWTRDFGPISVYVDGKVC
                     MYDFGFNAWGGKFAFDLDNKLTAKLLEAGAFGPDRLYSDRKNF"
     gene            complement(438..1433)
                     /locus_tag="PKY68_08220"
     CDS             complement(438..1433)
                     /locus_tag="PKY68_08220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOH25054.1"
                     /translation="MKGPEISVFIPVYNAGAYLKPTLESVLNQSFTDFELLVVDDSST
                     DGSWLILQDYAGREPRMRILQKEHGGNVPKSWNVALPLLRGKFVFYLSQDDLLSPDCF
                     EQMIRRQRECDADCVLPDMLRFTRDPSDAVQEQALTAPDADYNRILDGRSAFLLSLDW
                     SIHGFILCRRQFYTEEEFDESTFNSDEYVTRKIFLKSGQVAFSGGRFYYRRGNPNSIT
                     DPGHPFRYQVLRTNRRLLRLMQEQGFDRQELYKYRRQVFDTFCGLHRYYLNHKNSYVF
                     TEQSSIQALFAEEYEALHKERKATKRAYRHSGVKQRIRAALMLCSYGVFVLFNKK"
     gene            complement(1420..>2908)
                     /locus_tag="PKY68_08225"
     CDS             complement(1420..>2908)
                     /locus_tag="PKY68_08225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH25055.1"
                     /translation="GYIEELHAAWPGKIAPGQIVAISNPLTGRAKISARKPQIIYAGR
                     LSREDKRIDRLLRIWKLIQDRVPGWSFRIIGEGPEKEKLRNYAKKLGLKNLSFESPRV
                     DDSLYAEASIICLSSSYEGYGMVLLEARQAGIVPVAFDCSAGVRDVITHQRNGLLVPV
                     FDEEAFAESLLKLIGDENLRNRLSQQAIEDTRQNDMERIGPLWTALFEELLSQDNILE
                     SNNATTELEVLISTYAKPLQPIVDRLPDPMKGVHYLISCQGVQDPEPLDFQRKDVSIS
                     FTGSTGIAANRNNSIKAARGKIVWLLDDDVLFAQSDLQRILECYEADPGIDLACFQIR
                     TPEGRPYKHYKEQAFRMKSLDDVRDVSSIEITFRLASVRAKGLVFDERFGLGRQWPCS
                     EEFLFLAACLKAGLHIHYFPYPVVEHPYESTIKKMSQYSPVLLEKSGAVNAVLYGRKA
                     YLRNLVSLIKARKAIKNENMSACDFLRIKNRGSRKLLHSNRDERS"
BASE COUNT          739 a          726 c          694 g          749 t
ORIGIN      
        1 caaagttctt ccggtcggag tagaggcggt ccggaccaaa ggctccggcc tccaaaagct
       61 tggcagtcag tttgttgtca agatcaaaag caaattttcc accccatgca ttaaaaccaa
      121 aatcatacat acaaacttta ccgtcaacgt aaacggaaat gggtccgaaa tcccgggtcc
      181 aggtatcgtt gcagggcagt tcatacaaaa aaagcttttg cccgctttgt ggagcaattt
      241 ttgcccgcac tgaatttggg tggctgcata ctaccaacag gggctcgtag cgaagaatat
      301 gccgtgcaat ttcaatataa cagacttcta cttcttccag attatctgcc caatcgctgt
      361 ctttatgcgg ccaggtcagc tgaacaaagg cttgttttgc ccattcggga ggtaatatga
      421 tttgccggtg tttcatgtta ctttttattg aataaaacaa acactccata cgaacatagc
      481 atcaaagccg cccgaattct ttgtttaacg cccgaatgcc ggtaggccct tttcgtcgct
      541 tttctttctt tgtgcaatgc ctcgtattcc tctgcaaaaa gggcttgaat gctgctttgt
      601 tctgtgaaaa cgtagctgtt tttatggttt agataataac ggtgcagacc gcagaaagta
      661 tcgaagacct gccggcggta tttgtacaat tcttgcctgt caaagccctg ctcctgcatc
      721 aaccgcaaaa gtcgtcgatt ggttctgagc acctgataac gaaagggatg tcccggatcg
      781 gtaatgctgt tgggattgcc tcttctgtaa taaaaacgcc ctccggaaaa agccacctgc
      841 ccgcttttta aaaagatctt ccgggtgacg tattcatcgc tgttgaaagt gctttcgtcg
      901 aattcttctt cggtgtaaaa ctgcctgcga cagagtataa aaccgtgaat actccagtcg
      961 aggctgagta aaaaggcaga gcgtccgtcg agtatccgat tgtaatcggc atcgggcgct
     1021 gtaagagctt gctcctgaac agcatccgag ggatcccggg taaagcgcag catatcgggc
     1081 aaaacgcaat cggcatcgca ttccctctga cgacggatca tttgttcaaa gcaatcgggc
     1141 gacaacaaat cgtcctgtga aagataaaac acgaatttac ccctcaacag cggaagcgcc
     1201 acattccagg atttgggcac attccctccg tgctcttttt gaagtattcg cattcgcggt
     1261 tcacgcccgg cgtaatcctg caatataagc caactgccgt ctgtagaact gtcgtcgacg
     1321 accagcagtt caaagtcggt gaagctttga ttcaaaacgg attccaaggt cggcttcaga
     1381 taagcgccgg cgttgtaaac cggtataaag accgaaattt caggaccttt catctcggtt
     1441 tgaatgaagg agtttacgac tgccgcggtt tttgatacgc aaaaaatcac aggctgacat
     1501 gttttcgttc ttaatggctt tgcgggcttt gatcagcgaa accaagtttc tcaaataagc
     1561 ttttcttccg tagagcaccg cattgacggc ccccgatttt tccagcaaca ccgggctgta
     1621 ttggctcatt tttttaatgg tgctttcgta aggatgttct accaccggat aaggaaaata
     1681 atgaatgtgc agacctgctt tgaggcaggc cgccaggaac aggaattcct cgctgcaagg
     1741 ccattgcctg cccagtccga aacgttcgtc aaaaacaaga cctttggccc tgacggaggc
     1801 cagcctgaag gtaatttcta tagagcttac atcccgaaca tcgtcgagac tcttcattct
     1861 gaaggcttgc tctttgtagt gcttgtaggg cctgccttcg ggggttctga tttggaaaca
     1921 ggccaggtca ataccaggat ctgcttcgta acattcgaga atacgctgga ggtcggactg
     1981 tgcaaaaagc acatcgtcgt cgagcaacca gacgattttg cctcttgcgg ccttgatgct
     2041 gttgttgcga ttggcggcaa tccccgtgct gccggtaaaa ctgatggaga catctttacg
     2101 ttgaaaatcg agaggctccg ggtcttgaac tccctgacag gaaataagat aatgcactcc
     2161 cttcatggga tctggcaatc gatcgacgat gggctgcaag ggcttggcgt aagtagaaat
     2221 caatacttcc agttcagtag tcgcattgtt ggattcgagg atattatcct gagacagcag
     2281 ctcctcaaac aaagccgtcc acaaaggacc tatgcgttcc atatcgtttt gccgggtatc
     2341 ttcgatggct tgttgcgata gcctgttgcg aagattctcg tccccgataa gtttgagcag
     2401 gctctcggca aaggcttctt cgtcaaagac cggaaccagc aaaccgttac gctgatgagt
     2461 gatgacatcg cgcacacccg cgctgcaatc gaaagccacc ggaacaatac cggcctgacg
     2521 ggcttcgagc aataccatac cgtatccttc ataagacgag gataaacaaa taatggaagc
     2581 ctcggcataa aggctgtcgt ccacacgggg agattcgaaa ctcaagtttt ttaagcccag
     2641 tttttttgcg taattcctga gcttttcctt ttcgggacct tcgccgataa tccgaaaaga
     2701 ccagccgggc accctatcct ggatcagctt ccaaatacgt aataaacggt cgatcctctt
     2761 gtcttccctg ctcagacggc cggcataaat aatttgaggc ttacgggcag aaatcttcgc
     2821 ccggccggtc aggggattgg aaatggccac aatttgcccc ggtgcgatct ttcccggcca
     2881 ggcggcatga agttcttcga tataaccc
//
LOCUS       DAOSGL010000289         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_32968_length_2882_cov_4.632119, whole genome shotgun sequence.
ACCESSION   DAOSGL010000289 DAOSGL010000000
VERSION     DAOSGL010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32968_length_2882_cov_4.632119"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /locus_tag="PKY68_08230"
     CDS             <1..1890
                     /locus_tag="PKY68_08230"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOH25056.1"
                     /translation="QDTTNSFSWYDSYPYSIISVFALHPIYAAPSLLPALEDRDKAAS
                     FEERAKQLNLSESVKYEEVLSLKWEYFRLLFDQEQGKIIQSVDYKRFLKENSHWLRPY
                     AAYCYLLRKLNNPDPKAWGEYSNYDSESISRLCDPRQSWHKDIAIHYYIQFQLHCQLE
                     QVHDYAAQKGILLKGDIPIGVNRFSVEAWQHKNLFRLDLQTGAPPDEFSDQGQNWGFP
                     VYDWNEMHRWQYKWWRKRLQHMAKYFDAYRIDHILGFFRIWSIPTDSTSGLLGYFKPA
                     LPYTPKEIRAFGLDFDRQTMCKPLVDEVLLGEYPGLEIFSYLRSPGNQKRHSSKKESP
                     APGSKPDQTGNHQRYLLKEELDTQSKVRNYFGDSTQKEISRLREALCRMCTEVLFIED
                     KDKAGRYHPRILGNQTAAYRCLSEKQQKAFDRLYEHFYFERHNEFWKEQAMRSLPELL
                     SSSSMIACGEDLGMIPASVPEVMEQLCMLSLELERMPKTFGVEFEALDRIPYLSVCTT
                     STHDMSPLRAWWEENPLQAGRYWQSVLHEQGDAPLECSADLAYRILQHHLSSNAMWVI
                     LPWQDWLALHNDLRKTRAETERINVPENSKHRWDYRIHINIEDFLNSTTLNTTIRRMI
                     ADSGR"
     gene            complement(1963..2415)
                     /locus_tag="PKY68_08235"
     CDS             complement(1963..2415)
                     /locus_tag="PKY68_08235"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HOH25057.1"
                     /translation="MNQAYAFDGSESTKTSKEAETFKTAIENRQFVFQAQTAHPMAWR
                     SVNLSYGFHLKVSPDNIEAYLPYYGRAYKAPLSYEESGVKFNSHDFDYQVKEGKDGRL
                     DITIKPKDVTGKNYILYLSAFPEGSCSLSVQDPDRQHIHFNGILELPE"
BASE COUNT          815 a          622 c          652 g          793 t
ORIGIN      
        1 caggacacga ccaacagttt ttcctggtac gattcgtatc cttacagcat tatttcggtt
       61 tttgccctgc atcctattta cgcagcccct tctttgttgc cggcacttga ggacagagac
      121 aaggcagcgt ctttcgaaga aagagctaag cagttgaact tgagcgaaag tgtgaagtac
      181 gaagaggtgt tatcgctcaa atgggagtat ttccggctgc tttttgatca ggaacaaggg
      241 aaaataatcc aatcggttga ttataagcgt tttctaaaag aaaacagcca ttggttgcgg
      301 ccctatgccg cttattgcta tttattgcgt aagctgaata atcccgatcc caaagcctgg
      361 ggggaatatt ccaattacga tagcgaaagc atttccaggc tttgcgaccc caggcaatcc
      421 tggcataaag acattgccat tcattattat attcagtttc aactccactg ccagctggag
      481 caggtacacg actatgcagc tcaaaagggc atattgctca agggcgatat tccgataggc
      541 gtcaatcgat tcagcgtgga agcctggcaa cacaaaaact tgtttcgcct cgatttgcaa
      601 accggcgctc cacccgacga attttccgat caggggcaga attggggttt cccggtttac
      661 gactggaacg aaatgcatcg ttggcaatac aaatggtggc gcaagcgttt gcaacatatg
      721 gcaaagtatt tcgatgctta ccgtatcgat catattttgg gttttttccg tatctggagt
      781 attcccaccg attcaacttc gggtttattg ggttatttca agcctgcctt gccctacact
      841 ccgaaagaaa tcagggcttt cggtctggat ttcgaccggc aaacgatgtg caagccttta
      901 gttgacgaag ttctgcttgg ggaatatccg gggctggaga ttttttctta tctcagatcg
      961 ccgggtaatc aaaaaagaca ttcgtcgaaa aaagaatcgc ccgctcccgg gtcaaagccc
     1021 gatcagacgg gtaatcatca aagatatttg ttgaaagaag aattggacac ccaatccaaa
     1081 gtgaggaatt atttcggcga ttctactcaa aaagaaattt cccggctgcg ggaggcttta
     1141 tgccgtatgt gcaccgaagt cctttttatc gaagacaaag ataaagcagg tcgttaccac
     1201 cccagaattc tcgggaatca gacagctgcc tatcgttgcc tgagcgaaaa acagcaaaag
     1261 gccttcgatc gtctttatga gcatttttat ttcgaaagac ataacgaatt ctggaaagag
     1321 caggctatga gaagtttgcc cgaattgctt tcgtcgagca gcatgatcgc ctgcggcgaa
     1381 gatttgggta tgataccggc ctctgtgccc gaagttatgg agcaactctg tatgctgagc
     1441 ctcgaactgg agcgtatgcc caaaactttt ggcgttgaat ttgaagctct ggatcgtatt
     1501 ccctatttgt cggtgtgcac aacttccacg cacgatatgt cgccgttaag ggcctggtgg
     1561 gaagagaatc ctttacaggc cggacgctac tggcaatcgg ttttacatga acaaggcgat
     1621 gcgcctctcg aatgcagtgc cgatctggct tacaggattt tgcaacatca cttgtcttcc
     1681 aatgccatgt gggtgatact tccctggcaa gattggctgg cgctgcataa cgatttgaga
     1741 aaaacccggg cggaaacgga gcgtatcaac gtgccggaga actcaaaaca tcgttgggat
     1801 tacagaatac atataaatat cgaggatttt ctgaattcca ccactctaaa cactaccatt
     1861 cgccgaatga ttgccgattc cggcagataa ttttcagggt ttagtaaatt caattagtaa
     1921 atgcaactgg ttgcattggg atgttgaatc tatgcttgcc aattactccg gcaattccag
     1981 aatgccgtta aagtggatgt gttggcggtc gggatcctgg accgagaggc tgcaactgcc
     2041 ttcgggaaag gctgagagat acaatatata gtttttaccg gtgacgtctt ttggcttaat
     2101 ggtaatatcc agcctgccgt ctttgccttc ttttacctga taatcaaaat cgtgggaatt
     2161 gaatttcacc ccgctttctt cgtagctgag cggagcttta taggctcttc cataataggg
     2221 aaggtaggct tctatattat caggacttac cttaaggtgg aacccgtaac tcagattgac
     2281 gcttctccag gccatgggat gtgccgtctg ggcctgaaaa acaaactgcc ggttttcgat
     2341 ggcggtttta aaagtctcgg cttctttgga ggtctttgtc gattcggacc cgtcaaaagc
     2401 gtaggcttga ttcatacaca aaccacaaaa aagcagaatc ggaaacagtg atttaaagac
     2461 cgtcatgagg gaaaaattta ttggtaaaaa tacagattaa acgccatatc ctctattttc
     2521 cggacataaa aatgaaaaac acaaggctaa aaatctaaaa caaaagaagt tttgaaagat
     2581 ttccacacaa taaagagctg atttgtaata cacgagggcg aatttgtata aaattaaccg
     2641 ccggaaaagg gcgttataca ataaagttta aaaaaaactc ttacatttgc atgtcttaac
     2701 cataggaaaa acggtattca cagaccttaa acctgaatac atatgcgcta agtttttgtt
     2761 ataattcttt tctttaattt aaaacctatt ttcaaataac cttaattatc aacaatcaaa
     2821 acacgtatta tgaatcttgt atcatttttc agacgagtat tcgtgacctc atttctggcc
     2881 gc
//
LOCUS       DAOSGL010000290         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_33009_length_2880_cov_4.237168, whole genome shotgun sequence.
ACCESSION   DAOSGL010000290 DAOSGL010000000
VERSION     DAOSGL010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33009_length_2880_cov_4.237168"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PKY68_08240"
     CDS             complement(<1..851)
                     /locus_tag="PKY68_08240"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25058.1"
                     /translation="MVCFYNDATGRGLIAGKTLLCLVFILLFLPGTLAQRRVDSPDRG
                     IVAIPKSANTIYLSWRHFATDPDEIAYNLYYKTSAEGALTKLNSNPVTGSTNYTANLS
                     TSASAYTFVVKSVLEGVEKDEAGSFTLPQNSGIHRIVRDFDFEPLPDGHPKMAMKFCW
                     PADLNGDGAYDYVIDRQDYGASMMEDGGEATAGYPYPKVEAYSSEGVFLWRIDMGPNI
                     KICSGHDDMVTAYDMDGDGKAEVLMAVSEGTTFADGTVIKGSNGQVTDYRTIAGPAPQ
                     WIAVVNG"
     gene            978..2201
                     /gene="pepT"
                     /locus_tag="PKY68_08245"
     CDS             978..2201
                     /gene="pepT"
                     /locus_tag="PKY68_08245"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOH25059.1"
                     /translation="MQLIDRFLRYVKMDTQSDDATNLVPSTPSQMEFAKKLAEELRQI
                     GLQEVSLDKNAYVMATLPANTDKVRPVIGFIAHMDTSPDMPGRKVQPQIVRKYNGQDI
                     VLNAKENIVLSPQQFPELLHYIGQDLICTNGLTLLGADDKAGIAEIVTAMEYLLAHPE
                     IKHGKIRLGFTPDEEIGRGADHFDVKAFGADFAYTLDGGEIGELEFENFNAASAKVRI
                     KGLNVHPGYAKHKMRNACRIAQQFVSMLPRHETPEHTEGYEGFYHLTAMQGTVEEATL
                     DYIIRDHDRDRFERRKKEMQHLVNKINQEFGEGTATLTLKDQYYNMREKIDPDNMHVV
                     QLAIDAMKAVGVEPKIKPIRGGTDGARLSYMGLPCPNIFAGGLNFHGRYEFIPIPSMR
                     KAMEVVVKIAEINCN"
     gene            2287..2880
                     /locus_tag="PKY68_08250"
                     /pseudo
     CDS             2287..2880
                     /locus_tag="PKY68_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
BASE COUNT          747 a          720 c          718 g          695 t
ORIGIN      
        1 tcgccgttga cgacggctat ccactgggga gcgggtccgg ctatagtccg gtagtccgtc
       61 acctggccgt tgctgccttt gatgactgtt ccatcggcaa aggtggtgcc ttcgctgacg
      121 gccatcagca cttcggcctt gccgtcgccg tccatatcgt aggcagtcac catgtcgtcg
      181 tgtcccgagc agatcttgat attgggtccc atgtcgatgc gccacaaaaa gacgccttcg
      241 ctgctgtaag cttccacttt gggataggga taaccggcag tagcctcgcc gccgtcttcc
      301 atcatggaag ctccgtaatc ctgacggtct atcacataat cgtacgcacc gtcgccgttt
      361 aagtcggccg gccagcaaaa tttcatagcc attttgggat ggccatcggg caggggttcg
      421 aaatcaaaat cccggacgat gcggtgtatt ccactgtttt gaggcagagt gaagctgccc
      481 gcttcgtctt tttccactcc ctcgaggacg gatttgacta caaaagtata ggcggacgca
      541 cttgtagaca gattagccgt atagttggtg cttccggtaa ccgggttgct gttgagtttg
      601 gtcaatgccc cttcggccga ggttttgtag taaaggttgt aggctatttc gtcgggatcg
      661 gtagcaaaat gccgccatga cagataaata gtgttggccg atttgggtat ggccacgata
      721 ccgcgatccg gagagtccac tctgcgttga gccaatgtgc ccggcaggaa aagaaggata
      781 aaaacaagac agagcaaggt tttcccggca atgagtcccc ggccggtggc gtcgttataa
      841 aaacatacca tggtctttgt gaaagataaa atctgtcgca aagataatta aaaagcgaat
      901 ttatatacat agacatacaa aacagggaga gcatcgggaa attttgacta tttttgctca
      961 tcgcaaaaaa gagtattatg caattgatag atcgatttct gcgttacgtc aagatggata
     1021 cccagtctga cgacgctacc aatttggttc ccagtacccc ttcacaaatg gaatttgcaa
     1081 aaaaactggc cgaagaactc cgtcaaatcg gtttgcaaga ggtcagcctc gacaaaaatg
     1141 cctatgtaat ggccaccttg ccggccaata ccgataaagt ccgcccggtg atcggtttca
     1201 ttgcccatat ggataccagt cccgatatgc cggggcgcaa ggtgcaacct cagatcgttc
     1261 gaaaatacaa cggccaggac attgtcctta atgctaaaga aaacattgtg ttgtctccgc
     1321 aacaatttcc cgagcttctc cattacatcg gtcaggatct catctgcact aacggactta
     1381 ctttgcttgg agcggacgac aaggccggca ttgccgaaat cgtcacggct atggagtatt
     1441 tgctggcaca tcccgaaatt aaacacggta aaatccgact gggctttact cccgacgaag
     1501 aaataggccg gggtgccgat cattttgacg tgaaagcttt cggcgccgat tttgcttaca
     1561 ccctcgatgg gggagaaata ggagagttgg aattcgaaaa tttcaacgcg gcttctgcca
     1621 aagtccggat caagggctta aacgtgcatc cgggttatgc taaacacaaa atgcgcaatg
     1681 cctgtcgcat agcccagcaa tttgtatcca tgttgccccg gcacgaaact cccgaacata
     1741 ccgagggtta cgaaggtttt taccacctta ccgccatgca gggcaccgtg gaagaggcca
     1801 ctctggacta catcatacgt gaccacgacc gcgaccgttt tgaacgcagg aaaaaggaaa
     1861 tgcagcacct ggtcaataaa atcaatcagg aatttggtga aggtactgct actctgacgc
     1921 tcaaagacca atactacaac atgcgcgaaa agatcgatcc cgacaatatg catgtggtgc
     1981 agttggccat agacgccatg aaggcagtgg gcgtggaacc taagattaaa cccatacggg
     2041 gaggcaccga cggagcccgc ttgtcttata tgggcttgcc ttgtcccaat atttttgccg
     2101 gcggtctgaa tttccacggt cgctacgaat ttatccccat cccctccatg cgcaaagcta
     2161 tggaagtggt ggttaaaatt gccgaaatca attgcaacta atttttttct cagcatgaaa
     2221 acaactcctt ttacagacat ccacctgggg ctcaaagcca aaatgcatga atttgccggt
     2281 tacaatatgc ccattgaata taccggtatc ctgcccgaac atctttgtgt ttgcgagggt
     2341 gtgggcgtgt tcgatgtttc ccatatggga gaattcgccg tcagcgggcc cagggcactg
     2401 gacttccttc agcaagtttg ctccaacgac gtgtcggtat tgaccgatgg ccaggctcag
     2461 tacaattatt tccccaacga gcaaggcggc atagtggatg acttgttggt gtatcgcata
     2521 aacaagaacg aatacctgct ggtggtgaat gcctccaaca tcgaaaagga ctggattggt
     2581 gttgttcgca caatcagagc ggcgccaaac tcgaaaacct ctccaaccgc actgcccagc
     2641 tggccatcca aggcccaaaa gcattggatg tgttgcaaaa attaagtccg gtcgatctca
     2701 aagccattcc ttattatcat tttacttatg gcaagatggc cggtatagat caggtcttga
     2761 tatccaatac aggctatacg ggcgccggcg gttttgaact ctatttcaag cccgaagacg
     2821 gattgaaact ctgggaagcc atttttgaag ccggagccga atacggcatt cagcccattg
//
LOCUS       DAOSGL010000291         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_33121_length_2872_cov_3.601349, whole genome shotgun sequence.
ACCESSION   DAOSGL010000291 DAOSGL010000000
VERSION     DAOSGL010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33121_length_2872_cov_3.601349"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PKY68_08255"
     CDS             <1..885
                     /locus_tag="PKY68_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH25060.1"
                     /translation="RFTRAEFIDDKAVVDALFETAENWIKSKGMNGAMGPLGFSNLDS
                     QAMLVEGFDQLASVASVYHLPYYKDHLERLGYRKKTDWIEFRLSLTSEIPEKAVRVAD
                     IIQQRYQLSVIRMKSKRIMRKFGRQIFRLFNKSFYELFSFAPLDEKMIDHVLDTYLPV
                     INPEYIHIVVNNEQKIVGFIVPVPSLSKSMQLAQGKLSLRAIWSIMRSRKKNDTVDLF
                     LAGIDPEYQAKGVTSLLITKAQEVMIKNGITTVETTGILEDNFKAIQHWKNYEHVQNK
                     RRRCFIKDFDKQDSESEA"
     gene            complement(897..1358)
                     /gene="smpB"
                     /locus_tag="PKY68_08260"
     CDS             complement(897..1358)
                     /gene="smpB"
                     /locus_tag="PKY68_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOH25061.1"
                     /translation="MKLSRDILIKNKRAAFNFELLDRFTAGIVLTGTEIKSIRLGKAS
                     LSDSYALMIKGELWIKNLQISEYDNGSYNNHNPRRDRKLLLTKRELRKIERLTRETGF
                     TVVPLKLFINEKGWAKLEIAVAKGKKAHDKRESIKEKDDRRQMDRYIKSKL"
     gene            complement(1358..1930)
                     /locus_tag="PKY68_08265"
     CDS             complement(1358..1930)
                     /locus_tag="PKY68_08265"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOH25062.1"
                     /translation="MNKDFFRRIWRIIISPAATWKEITTETAENKPYLSAYFYPLLTL
                     VGMAAFFSSFIHTQETFRIELVQGIRIFIRVFAAFFASYFLSSFVLDRLFVRWLGLAP
                     DKIKTEKLCVYSLTPLLLVRFFTFLVKDLFFLYIFVVYTVYVVWEASGVLYEMPGKQR
                     GRFTALTTVVIILSPVLIELILIKLLPGLS"
     gene            complement(1923..2525)
                     /locus_tag="PKY68_08270"
     CDS             complement(1923..2525)
                     /locus_tag="PKY68_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOH25063.1"
                     /translation="MIDTYKTIANRSEGLYKDKGSKFLAFAIPVEGIEEVNLELEQFR
                     KSYFDARHVCYAYMLGVDGEQYRVYDDGEPSGTAGRPILGQIRSFGLSNVLVVVVRYF
                     GGVLLGTGGLTVAYKTAAAAALSNARILEKTLDAQISLSFEYPYTNEVMSIIKDCEAQ
                     IIDPKYEELCSMSLSIRQSRLNELKERLSKVESVLITSDE"
     gene            complement(2552..>2872)
                     /locus_tag="PKY68_08275"
     CDS             complement(2552..>2872)
                     /locus_tag="PKY68_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOH25064.1"
                     /translation="KPGRYDDKDPLGVLDVNISSRLILDRILGIKDLRSDKRIDFVGG
                     IRGLKELERRVDSGEMALALALYPVSMKQLIDIADSGNIMPPKTTWFEPKLRSGLVIH
                     TLDD"
BASE COUNT          804 a          685 c          628 g          755 t
ORIGIN      
        1 cgtttcacca gagcggaatt catcgacgac aaggcagtgg ttgacgcttt gtttgagaca
       61 gcggaaaatt ggataaaatc caagggtatg aacggagcaa tgggacctct gggcttttcg
      121 aatctcgact cacaggctat gctcgtagag ggctttgatc agcttgcctc tgtggcatcg
      181 gtttatcatc tgccttatta caaggaccat ttggagcgtc tgggctacag aaaaaaaacc
      241 gactggatag agtttcgatt aagcctgaca tccgagattc ccgaaaaggc tgtccgggtg
      301 gcggacatca tacaacaacg ttatcaactc agcgttattc gcatgaagtc caagcgcatc
      361 atgagaaaat tcggccgcca aatttttcga ttgttcaaca agtcttttta cgaactcttt
      421 tcttttgctc ccctggacga aaaaatgata gaccatgtgc tggacactta cctaccggta
      481 atcaatcccg agtacataca catcgttgta aataacgaac aaaaaatagt cggcttcatt
      541 gtgccggtgc cttcgctgag caaatccatg caacttgccc aaggcaaatt gagtctccgg
      601 gctatatggt ctataatgcg cagccgtaaa aagaacgaca ccgtagatct ttttctggcg
      661 ggtatcgatc cggagtatca ggctaaagga gtcaccagcc tgcttatcac caaagctcag
      721 gaagtgatga ttaagaatgg aattacgacg gtcgaaacca caggtatact cgaagacaat
      781 ttcaaagcca tacaacactg gaaaaattac gagcacgtac aaaacaaacg cagacgttgc
      841 tttataaaag actttgacaa acaggactct gaaagcgaag cctaaactgt tgtattttac
      901 aatttgcttt tgatgtaacg atccatctgg cgacggtcgt ctttttcctt gatgctctcg
      961 cgtttatcgt gggctttttt acccttggcc actgcgattt ccaatttggc ccagcctttt
     1021 tcgttgatga agagctttaa cggaacgacg gtgaagcccg tttcccgggt aagtcgttct
     1081 attttgcgca attctctttt agtcagtagc agcttgcggt cgcgtctggg attgtggttg
     1141 ttgtagctgc cgttatcgta ttcgcttatc tgaagattct taatccagag ttcaccttta
     1201 atcatcaggg catagctgtc cgaaagactg gccttgccca ggcgtatgga tttgatctcg
     1261 gtgccggtca ggactatacc ggccgtgaag cggtccagca attcaaaatt aaaagcagcc
     1321 cgcttgtttt tgatcaatat gtccctggat aatttcatta agatagaccc ggcaacaact
     1381 tgataagaat taattcaatc agcacgggag acaatattat tactacagtt gtcaaagccg
     1441 taaagcgacc tctttgcttg cccggcatct cgtacaacac gccggatgcc tcccatacga
     1501 cgtacaccgt gtaaacgaca aaaatataca gaaaaaacaa atccttgacc agaaacgtaa
     1561 aaaaccgtac aagcaacaag ggcgtcagcg aatatacgca gagcttctcg gtttttattt
     1621 tgtccggagc caagcccagc caacgaacaa acaaacgatc cagtacgaaa gaggacaaaa
     1681 agtaggaagc aaaaaaagcc gcaaaaaccc ggataaagat tcgaataccc tgtaccagtt
     1741 cgatccggaa agtttcctgt gtatgaataa acgaactgaa aaaagcggcc ataccaacca
     1801 gtgtcagcaa aggataaaaa taagcactca aatagggctt attttcggcc gtttctgttg
     1861 ttatctcctt ccaggtagca gccggactaa ttataatccg ccagatgcga cggaaaaaat
     1921 ctttattcat cgctggttat aagcactgat tccacttttg ataaacgttc tttcagttca
     1981 ttcaatctac tctgcctgat gctcaaactc atcgaacaaa gttcttcgta tttgggatcg
     2041 atgatttgag cttcgcaatc cttgataatg ctcatcactt cgttggtata gggatactca
     2101 aaactaaggc tgatttgggc atccagcgtt ttctccagga ttctggcatt ggaaagtgcc
     2161 gcggcggcgg ctgttttgta ggcaacggtc aagcctccgg ttcccaataa aacacctcca
     2221 aaataacgca ccaccaccac caggacattg ctcaaaccaa aagaacggat ttggcctaaa
     2281 atcggacgtc cggcagttcc cgaaggctct ccgtcgtcgt aaaccctgta ttgctctcca
     2341 tcaactccca gcatgtaggc atagcacaca tgacgggcat caaaatagct ttttctgaat
     2401 tgctccaact ccagattcac ttcctcaatt ccttctactg gaatggcgaa ggccagaaat
     2461 ttgctgcctt tgtctttgta gaggccttcg ctgcgattgg ctatggtttt gtatgtatca
     2521 atcatggtac aatgcgggcg ttcagcacct ttcagtcgtc taaggtatgt attaccaatc
     2581 ctgaacgtaa tttaggttca aaccaggtgg ttttgggagg cattatattg ccgctgtcgg
     2641 caatatcgat gagttgcttc atcgacacgg ggtatagagc aagagccagg gccatttcgc
     2701 ccgagtccac gcggcgttcc aactctttaa ggccacgaat accgccgaca aaatcgatgc
     2761 gtttgtcgct gcgcaaatct tttatgccca aaatacgatc caatatcaga cgcgatgaaa
     2821 tgttgacatc caatacgcct aagggatcct tgtcgtcgta tcttcccggt tt
//
LOCUS       DAOSGL010000292         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_33159_length_2870_cov_4.751687, whole genome shotgun sequence.
ACCESSION   DAOSGL010000292 DAOSGL010000000
VERSION     DAOSGL010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33159_length_2870_cov_4.751687"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PKY68_08280"
     CDS             complement(<1..1189)
                     /locus_tag="PKY68_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH25065.1"
                     /translation="MRKLVLFIALFLSLTSLQAYQPEISIAGFFPLEGSGRSVYNFNP
                     GWRFYRGDVPEARNLDFDDQAWPIVNLPHTVQLMPAEGSGGRNYQGKAWYRKRFVPDS
                     SLQGQQISLYFEAVMGRSTVYLNGKQVLEHLGGYLPFAVNLNEFGVEAGDTCLIAVCA
                     DNSDDKSYPPGKTQYTLDFAYHGGIYRDVWMIAKAPVHITDPVVESHQVAGGGVFVHY
                     DNISEQSAQVYVDTEIANKDNQKRSVVLETALYDTEGKLVKKVRNNLSLEAGEKKTIN
                     QLIEVKQPYLWSPASPYLYRIHSQVRQGKIMLDGGISRIGIRLAEFKGKEGFWLNGKP
                     YGQLIGTNRHQDFAYVGNAMPNSQHWRDVKKLREAGCEIIRAAHYPQDPAFMDACDEL
                     GLYV"
     gene            complement(1213..>2870)
                     /locus_tag="PKY68_08285"
     CDS             complement(1213..>2870)
                     /locus_tag="PKY68_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HOH25066.1"
                     /translation="QMTDPLSDLRFGGELCADGFVFKDTIAGVYAGTDFKAWRLESRK
                     PSKNHAVRLVFHTTQQVDGWHKGLDSIKSLIKPRSDKQLNADWWAKFWDRSFICIDPE
                     QPDTESWKVGRNYQLFRYMLACNLRGEAPTKFNGGLFTFDPVHVREDRPFTPDFRNWG
                     GGTFTAQNQRLVYFGMLKSGDFDLMIPQFEFYRKSLKNAELRSNHYWGHNGACYTEQM
                     EHFGLPNPSEYGWKRPENYDPGLEYNAWLEYQWETVLEFCLMILESHRYAGSDISPYM
                     EFIESTLTFFDEHYRFLARQRGSKELNADGQLILYPTSACETYKMAYNSTSCIAGLKV
                     VLESLLALENLSSDQRERWEKMLQTIPPISFREIEGKKVIAPALHWERINNTEVPQLY
                     PVFPWRIYNVVSEDKDTAINTYWLDPDVQKFYSHVGWKQYNIFAACLGLTEEAKKLTL
                     LKFKDAEHRFPCFWGPGFDWTPDHNWGGSASIGLQEMLLQTEGERILLFPAWPADWDV
                     HFKLHAPGQTTVEAELKNGKLLRLEVSPKERLADVENRLAIQE"
BASE COUNT          735 a          747 c          639 g          749 t
ORIGIN      
        1 ttacgtaaag tcccaactca tcgcaggcat ccataaaggc gggatcctga ggatagtggg
       61 cagcacggat tatttcacaa cccgcttcac gcagtttttt tacatcccgc caatgctggg
      121 agttgggcat ggcgttgccc acgtaagcaa aatcctgatg ccggttggtt ccgatcaatt
      181 ggccgtaagg cttgccattg agccagaaac cttcttttcc cttgaattcg gccagacgga
      241 ttccaatgcg gcttattcct ccgtcgagca tgatttttcc ctgtctgacc tgcgaatgaa
      301 tccggtacaa atagggagag gccggagacc ataaataagg ttgtttgact tcaataagct
      361 gatttatagt ctttttttca ccggcttcca ggctcaggtt gttcctgact tttttaacca
      421 gcttaccttc agtgtcgtat aaggccgttt ccaacaccac cgaacgtttt tgattgtctt
      481 tgttggctat ttcggtatcc acatatacct gagccgactg ttcggatata ttgtcgtaat
      541 gaacaaaaac acctcctccg gccacctggt ggctttccac cacagggtcc gtgatgtgta
      601 ccggtgcttt ggctatcatc catacatcgc ggtaaatgcc cccgtgatac gcaaaatcga
      661 gggtatattg cgttttaccc ggaggataag acttatcatc gctgttgtcg gcgcaaaccg
      721 ctatcaggca agtatcgccg gcttccactc caaattcgtt caaattaacg gcaaaaggca
      781 aatacccacc cagatgctcc aggacttgtt taccgttgag atagacggtg gaacggccca
      841 tcacggcttc aaagtacagg gaaatttgct gcccttgcag agaggaatcc ggaacaaagc
      901 gctttctgta ccaggctttg ccctgataat tgcgtccgcc gctgccttcg gccggcatca
      961 actggacagt gtggggtaaa tttactatcg gccaggcctg atcgtcaaaa tcaaggttcc
     1021 gggcttcggg gacatctccc cggtaaaatc gccatcccgg attgaagtta taaaccgaac
     1081 gtccactgcc ttccaagggg aaaaaaccgg ctatggatat ttccggctga taagcctgca
     1141 aggacgttaa agataaaaac aaagcgataa ataagactaa ctttctcatt attaaaaatt
     1201 tatagtatat gatcattcct gaattgccaa ccggttttct acgtcggcca acctttcttt
     1261 ggggcttacc tccaggcgca gcaacttacc gtttttcaat tcggcttcga cggtagtctg
     1321 tcccggagca tgcaatttaa aatgcacatc ccagtcggca ggccaggcag gaaagagtaa
     1381 aatacgctcc ccttccgtct gcaacaacat ctcctgcaag ccgattgagg cactcccacc
     1441 ccagttgtga tcgggagtcc aatcaaagcc cggcccccag aaacagggga aacgatgctc
     1501 cgcatctttg aatttcagca aagtcagttt ctttgcctct tctgttaaac cgagacaggc
     1561 tgcaaaaata ttgtattgct tccagccgac atggctgtaa aacttttgta catcgggatc
     1621 cagccaatag gtattgatgg ccgtatcctt gtcttcggag acaacgttat atattcgcca
     1681 gggaaacacg ggatacaact gcggaacttc cgtattgttt attctctccc aatgcagagc
     1741 cggagcaatc actttctttc cttcgatctc acggaagctg ataggcggta tcgtttggag
     1801 catcttttcc caacgctccc gttgatctga actcagattt tccagggcca gcaaagactc
     1861 caacaccact ttgagtccgg caatacaact ggttgaatta taagccattt tataggtctc
     1921 acatgccgaa gtagggtaca aaatcaactg tccgtcggca ttcaattcct tgctaccccg
     1981 ctgccgtgcc agaaagcgat agtgctcatc gaaaaaagtc agcgtactct caataaattc
     2041 catataaggg ctgatgtcgc tgcctgcgta gcgatgactt tcgagtatca tcagacaaaa
     2101 ctccaacacg gtctcccatt ggtactccag ccaggcattg tattccagcc ccggatcata
     2161 gttttcggga cgcttccatc cgtattcgct cggattgggc aacccgaaat gttccatctg
     2221 ctcggtataa caggctccgt tgtggcccca ataatgatta ctccttaatt cggcgttttt
     2281 aaggctttta cggtaaaact caaattgagg gatcatcaaa tcaaaatccc cgcttttaag
     2341 catgccaaaa tacacgaggc gttgattctg agcggtaaaa gtgccacctc cccaattgcg
     2401 gaaatcaggc gtgaaaggcc ggtcctcgcg cacgtgaaca gggtcgaagg taaacaagcc
     2461 tccgttgaat ttggtgggag cctctccacg aaggttacaa gccagcatat agcggaataa
     2521 ctgataatta cgtcctactt tccagctttc ggtatctggt tgttccggat caatacaaat
     2581 aaaactgcga tcccaaaact ttgcccacca atcggcgttg agttgcttgt cgctgcgagg
     2641 tttaattaag cttttaatac tatctaagcc tttatgccag ccatcaactt gctgagtagt
     2701 gtgaaaaacc agacggacgg catgattttt ggatggcttc ctgctttcca gcctccaggc
     2761 cttaaagtcc gtaccggcgt aaactcctgc aatggtgtct ttgaacacaa aaccatcggc
     2821 acagagctca ccaccgaagc gaagatccga cagaggatcc gtcatttgtt
//
LOCUS       DAOSGL010000293         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_33192_length_2868_cov_5.136865, whole genome shotgun sequence.
ACCESSION   DAOSGL010000293 DAOSGL010000000
VERSION     DAOSGL010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33192_length_2868_cov_5.136865"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PKY68_08290"
     CDS             <1..896
                     /locus_tag="PKY68_08290"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOH25067.1"
                     /translation="VVRDAKRLAPAKAYGLVLWSHAMGWHPSTSALDPGNINPPTLRS
                     AAEIIEDEELIGWFGQDGYYHMETPVLASALEDQGLEFMLFDACFTASVEMLYDLRHC
                     ADYLIGSPAEVMGAGYPYKDFVKLVFREDLSTEALCRQLCQAYMTSYRANTTHPSAST
                     VLVKLSEMDSLAVCTRAIFEADPIPVSSIDLEGIQHYELMNPHLFYDLDGYLAAVSRY
                     PLFYNAFKNQLNRTVLYKDCTDQIYSIYSSSRWFDVDSYSGLSAYIPRYDLPYSQKIT
                     NLNQAYFQTAWAQATGQTAPR"
     gene            984..2558
                     /locus_tag="PKY68_08295"
     CDS             984..2558
                     /locus_tag="PKY68_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOH25068.1"
                     /translation="MNLKFVFGLALCSLVLSCTRTVSIPTKDQVGAAVLPGEIKPVKA
                     PFEMPVFKKPVFPDVEINIADRGAKAGEKITGIVQAAIDELSAQGGGKVVVPGGNWQS
                     GRIILKSNVNLHFAEGAEVYFSGEVEDYQPAVFTRNEGVEMMSLGALIYAYKQDNIAV
                     TGKGKLIGPPMDCAIREQVLKSGVIENVISADTPVEERIYTGLGGSPVLLPMFISPTS
                     CTNVYIEGVTLERTAFWNVVPVYCDGVVIRGITVNSVGIPRGDGIDVESSRNVLIEYS
                     TLNCGDDCFTMKAGRCEDGMRVNRPTENVVVRYCLAKKGHGGITCGSETAGMIRNLYV
                     HDCVFDETGAGIRFKTRRNRGGGGENIVYERIRMNLTGNAFVWDMLGSRTYVGLLADR
                     LPVRELTPLTPSYKNITAKDIIVERSSQFIKITGIPETPLTNLRLENVKAENCERLIQ
                     LRDVDQIHLKNVQVSSDDNNINVLDGRNILFEKVTFSNPEGNIVCLTEGELCRNIRFE
                     ECSPGKPENWEGTVLE"
     gene            complement(2640..2711)
                     /locus_tag="PKY68_08300"
     tRNA            complement(2640..2711)
                     /locus_tag="PKY68_08300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2676..2678),aa:Arg,seq:ccg)
BASE COUNT          730 a          750 c          715 g          673 t
ORIGIN      
        1 aggtggtccg cgatgccaaa agactggctc cggccaaggc ctacggtctg gtgttgtggt
       61 cgcacgccat gggctggcat ccatccactt cggcgctgga tcccggcaac atcaatccgc
      121 ctaccctgcg gtcggcagcc gaaataatcg aagacgaaga gttgatcggc tggtttggcc
      181 aggacggtta ttatcatatg gagacacctg tacttgcctc agccctcgaa gatcagggac
      241 tggaatttat gctctttgat gcctgcttta ccgcctcggt agaaatgctt tatgatttaa
      301 ggcattgcgc cgactacctg atcggttcgc cggccgaggt aatgggagcg ggttatcctt
      361 acaaggattt tgtcaagttg gtttttaggg aggacctgag cacggaagct ctttgcaggc
      421 aactttgtca ggcctatatg acgtcctata gagccaatac tacccatccg tcggccagca
      481 cggtgctggt taaactcagc gaaatggact ctctggcggt ttgcacccga gctattttcg
      541 aagccgatcc cataccggtg tccagcatcg atttagaggg tattcagcat tacgaactga
      601 tgaatccgca tttgttttac gacctggacg gctacctggc agccgtttcg cgctaccctc
      661 tcttttacaa cgcctttaag aatcagctca accggaccgt actttacaaa gattgtaccg
      721 atcaaatcta ttccatctac agtagttcgc gttggttcga tgttgatagt tactcgggct
      781 tgagcgccta cattccccgc tacgacctgc cctacagtca aaaaataacc aacctcaacc
      841 aggcctattt tcaaactgca tgggctcagg caacggggca aaccgctccc cgataaccac
      901 tcacggaaaa tttttcctgc ttttgcgact ttcgcttaat tgaatacatt tgcaggagtt
      961 ctcataatcc ttaatcaaca cttatgaacc ttaaatttgt attcggtctt gcactttgca
     1021 gcttggtgct gtcctgtacc cggaccgtga gcatcccaac caaagatcag gtgggagctg
     1081 ccgtgctccc cggcgaaata aagcccgtaa aggctccttt tgagatgccg gtattcaaaa
     1141 aacccgtctt ccccgatgtt gaaatcaaca ttgcagaccg gggagccaaa gccggtgaaa
     1201 aaatcaccgg tatagtccag gcggccatcg atgagctcag tgctcaaggc ggaggtaaag
     1261 tcgtggtacc cggaggaaac tggcaaagcg gacgcattat cctcaaaagt aatgttaacc
     1321 tgcattttgc cgaaggggcc gaagtctact ttagcggcga ggtcgaagat taccaacctg
     1381 ccgtattcac tcgtaacgaa ggcgtggaaa tgatgtcgct gggtgccctc atttatgcct
     1441 ataaacaaga caatattgcc gttacgggca agggtaaact tatcggaccg cctatggatt
     1501 gtgccatccg cgaacaggtt cttaaatcgg gtgttataga aaacgttatt tcggccgaca
     1561 cccccgtcga agagagaatt tacaccggac tgggcggcag ccctgtgctc ctacccatgt
     1621 ttatttcgcc cacttcgtgt accaatgtgt acatcgaagg agtgaccctc gaaagaaccg
     1681 ctttctggaa cgtagtaccc gtttattgcg acggagtggt tattcggggc atcacggtca
     1741 attccgtagg cattccccgg ggcgacggca tcgacgtgga atcgagccgc aacgtgctga
     1801 tcgaatattc caccctgaat tgcggcgacg actgttttac tatgaaagcc ggccgttgcg
     1861 aagacggtat gcgggtgaat agacccaccg aaaacgtggt agtccgttat tgcctggcca
     1921 aaaaagggca cggaggtatt acctgtggca gcgaaaccgc cggcatgatt cgcaatcttt
     1981 acgtgcacga ctgcgtcttt gacgaaaccg gggcgggcat ccgcttcaaa acccgtcgaa
     2041 accggggcgg cggaggcgaa aatatcgttt acgagcgcat ccgtatgaac cttacgggca
     2101 acgcctttgt gtgggacatg ctgggttccc gtacctacgt gggcctgctg gccgaccgcc
     2161 tgccggtgag ggaactgacg ccgttgactc cctcctacaa aaacatcacc gccaaagaca
     2221 tcatagtcga aagatcttct cagtttatca agatcaccgg catacccgaa acgcccctga
     2281 ccaatctccg tctggaaaac gtcaaggccg aaaactgcga acgtctgatc caactccggg
     2341 atgtggatca aatacatttg aaaaacgtgc aagtaagctc cgacgacaac aacatcaacg
     2401 tattggacgg ccgcaacatt ctcttcgaaa aagtgacttt cagcaacccc gaaggcaaca
     2461 tcgtctgcct gaccgaaggc gaactctgcc gcaacatccg cttcgaagag tgcagccccg
     2521 ggaagccgga aaattgggag gggacagttt tagagtaatt tcggagttaa cggaacccaa
     2581 ctatgcaaaa catcaaaaag ctaacagcct gtatcacaac tgttagcttt ttttgtttgt
     2641 aatcccgtgg ggagtcgaac cccaatcgat ggaaccggaa tccattattc tatccattga
     2701 actacgggat ctcacttttc ggtggacaaa gatagcgtta agtgagagca aaagcaaaat
     2761 tttatttttg cttttgccga gcgcagctat cttctcagac gaagtctgaa aagtagcgtt
     2821 aagtgaaacg agcgcagcta tctccccaga cgaagtctga aaagtagc
//
LOCUS       DAOSGL010000294         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_33227_length_2866_cov_5.122376, whole genome shotgun sequence.
ACCESSION   DAOSGL010000294 DAOSGL010000000
VERSION     DAOSGL010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33227_length_2866_cov_5.122376"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKY68_08305"
     CDS             complement(<1..789)
                     /locus_tag="PKY68_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOH25069.1"
                     /translation="MTLQQLQYIVAVERHRHFLKAAEECGVTQPTLSAMIQKLEDELD
                     VKIFDRSRQPVEPTEIGLQIIHQAKTVLSNAFQLRDMVNQQRDGLKGELHMGIIPTIA
                     PYLIPDFISGFVQKYPDIDLSISELQTRQIIEHLKHSELHMAILSTPLEDPDLLEIPL
                     YYESFVAYVSPREELSALDEISYHDMPLKKLWVMQEGHCLRNQVFHFCHDVQKRNNIY
                     EAGSIGTLVSIVDKSGGYTIIPELHIPFLSEEQAKNVRPLVRPQA"
     gene            900..1391
                     /locus_tag="PKY68_08310"
     CDS             900..1391
                     /locus_tag="PKY68_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006849328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOH25070.1"
                     /translation="MLSEKLHQAINKQINAEFWSANLYLSMSAWFADKGLPGFSHWMK
                     EQFKEEQEHAMRFFNYLVSRGAKVEIGAYAAVPVDWKDAKKVFEDTLHHEQVVTGMIN
                     DLYTLALEEKDYATQSMLKWFIDEQVEEEDTARGILDALCHIKDNSFGLYRLDKEMGE
                     RQG"
     gene            1399..1884
                     /locus_tag="PKY68_08315"
     CDS             1399..1884
                     /locus_tag="PKY68_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOH25071.1"
                     /translation="MSHRLIPLSGTKEEIYKQLLPQLRRLVEDEDNHIANLANITAAL
                     KSALGFFWVGFYLVDGAWLVLGPFQGELACTRIAYGKGVCGKAWELGSALVVDDVDKF
                     EGHIACSAKSRSEIVVPVIKNDKVIAVIDVDSEHLATFDDVDLRYLTEIARLVAGLPG
                     L"
     gene            complement(2037..>2866)
                     /locus_tag="PKY68_08320"
     CDS             complement(2037..>2866)
                     /locus_tag="PKY68_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOH25072.1"
                     /translation="LQALGISSFVLGNILKYREKGGVFRQKSDLAKIYGLNQDTYKKL
                     KPYILLPDVEQASPAAEQVSPGSERVSPAAEQTPRESVTFEQTKTWSEGAKYRLEEAK
                     YRLPESEKIIHQQTEIELTAPVANRALSAGDKKAYRPEAFSKVCRINTADTADLMQYP
                     GIGRYTANRILDYRNRLGGYCSPNQLDEISGIYPENLRMLKQCLETDSSEIRKLKLNH
                     SSLEQLRLHPYLNFYQARALIELRQARGKICSPEDLLFLEEFNEQDLNRLLPYLDCR"
BASE COUNT          732 a          678 c          673 g          783 t
ORIGIN      
        1 ggcctggggt ctgaccaaag gacgaacgtt tttggcttgt tcctcactca aaaagggtat
       61 gtgtaattcc ggaatgatgg tatagccccc gcttttgtcg actatgctta ccagggtgcc
      121 gatgctaccg gcttcataaa tattattgcg tttttgcacg tcgtggcaga agtgaaaaac
      181 ctgattacgc aagcaatgac cttcctgcat cacccagagt tttttgagag gcatgtcgtg
      241 ataagatatt tcgtccagag cggaaagctc ttcacgaggc gacacataag ccacaaaact
      301 ctcgtaataa agcggtattt ccagcaaatc ggggtcttcg agcggggtcg acaaaatggc
      361 catgtgcaat tccgaatgct tgagatgctc aatgatttgc cttgtttgca actcagagat
      421 actcaggtct atgtcggggt atttctgaac aaaacccgaa ataaaatcgg gaatcaggta
      481 aggggcaata gtgggaataa ttcccatatg caactcgcct tttaaaccgt cgcgttgctg
      541 attaaccata tctcgcaatt gaaaagcatt gctcaatacc gtttttgcct gatgtatgat
      601 ttgcagaccg atttcggtag gctcaacagg ttgtctgctc cggtcgaaaa tttttacatc
      661 cagctcgtct tcaagctttt gaatcattgc gctcaaagta ggctgagtaa caccacattc
      721 ttcggcggct ttcaaaaaat ggcggtgtcg ttccacagcc actatgtact gcaattgttg
      781 taaagtcata ttattgaata aatctatgca aatatagaga ttatctgttt gatttataca
      841 atgccgggtg tatctttgcg acaagtttaa tcgaataatt aactctaata aatactacta
      901 tgttaagcga aaaattacac caagccatca acaagcaaat caatgccgaa ttttggtcag
      961 ccaacctgta tctgtcgatg tctgcctggt ttgcagacaa gggattgccc ggtttttccc
     1021 attggatgaa ggaacaattc aaggaagaac aggaacacgc catgcgcttt tttaattacc
     1081 tggtaagtcg cggtgccaaa gtagaaatcg gtgcatacgc tgccgtgccc gtagattgga
     1141 aagacgccaa aaaggtattt gaagacactc tgcaccacga acaagtggtt accggtatga
     1201 tcaacgacct ttatacgctg gctttggaag aaaaagatta cgccacccaa tcgatgctga
     1261 agtggtttat cgacgagcag gtcgaagaag aagacaccgc ccgcggcatt ctggatgcgc
     1321 tctgccacat aaaagacaac agctttggtt tgtatcgcct cgacaaagaa atgggcgaac
     1381 gacaaggata aaccattcat gagccatcgt ttgattcccc tttccggaac caaagaagaa
     1441 atctacaaac aattgctgcc gcagttgagg cgtttggtcg aggacgagga taatcacata
     1501 gccaatctgg ccaatatcac ggccgcacta aagtccgccc tgggtttttt ctgggtgggc
     1561 ttttaccttg tggacggggc ctggctggtg cttgggccct tccaaggaga gttggcttgc
     1621 actcgcattg cttacggtaa aggagtctgc ggcaaggcct gggagcttgg ttccgccctg
     1681 gtggtggacg acgtggacaa attcgaagga catatcgcct gcagcgccaa gtcccgttcc
     1741 gagattgttg ttccggtgat taaaaacgac aaggtaattg ccgttatcga tgttgatagt
     1801 gagcatcttg cgacttttga cgatgtcgat ctccgctacc tgacggaaat cgcccgactg
     1861 gtagccggac tgcccggact ttaagctcaa aagagcgcag cgtcatcgag tgcagcgtca
     1921 tcgaatcctg cgccctcgtg cgctgcgcca tcaagacctg caccattgaa tcttgcacca
     1981 ttgaatcttg caccgaactt tttatctttg gaatgtacta ttccggttcc ggcaacttac
     2041 ctacaatcaa gataaggaag caggcggttc aggtcttgct cgttaaattc ttccagaaaa
     2101 agtaagtcct cagggctgca tatcttgccc ctcgcctgcc tgagttcaat taaagccctg
     2161 gcctggtaaa aattcaaata gggatgcaac ctgagttgtt ccaggctgct gtggttcaat
     2221 tttagttttc gaatctcgct gctgtctgtt tccaggcatt gctttagcat cctcagattc
     2281 tcgggataaa tacctgatat ttcgtccaat tgattcggcg aacaatagcc gcccaggcgg
     2341 tttctgtaat ccaaaatacg gtttgcagtg taccgcccga tgccggggta ttgcatcaaa
     2401 tcggcagtat cggcggtatt gattctgcaa actttggaga aggcctccgg cctgtaggcc
     2461 tttttatctc cggccgacaa ggccctattc gccaccggtg cagttaattc aatttccgtt
     2521 tgttgatgaa ttattttttc cgattccggc agtcgatatt ttgcctcctc taaacgatat
     2581 tttgcccctt cggaccaagt ctttgtttgc tcaaacgtta ccgactcccg gggagtttgt
     2641 tcggccgccg gagaaactcg ctccgaccca ggggaaactt gttcggccgc cggagaagct
     2701 tgctctacat ccggcagtaa aatatagggt ttcaactttt tataggtatc ctgattcagt
     2761 ccataaattt tggctaagtc gcttttttgc ctgaagacgc ctcctttttc ccggtattta
     2821 agaatattcc ccaacacaaa agagctgatt cccaaagctt gcaatt
//
LOCUS       DAOSGL010000295         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_33686_length_2839_cov_4.535560, whole genome shotgun sequence.
ACCESSION   DAOSGL010000295 DAOSGL010000000
VERSION     DAOSGL010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33686_length_2839_cov_4.535560"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKY68_08325"
     CDS             complement(<1..341)
                     /locus_tag="PKY68_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HOH25073.1"
                     /translation="MSLHFLESELIINEDGSIFHLRLKPEQLAERVVLVGDPDRVSLV
                     ASKFDIVECRVRNREFHSVTGSYYGKRMTVLSTGIGTDNIDIVMNELDALANIDFEKR
                     SEKAEKKSLSIV"
     gene            complement(397..1371)
                     /gene="floA"
                     /locus_tag="PKY68_08330"
     CDS             complement(397..1371)
                     /gene="floA"
                     /locus_tag="PKY68_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861713.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOH25074.1"
                     /translation="MSPGFGLLILIPVIILLIIFFYYVPFFLWLSAKVSGVNISLIQL
                     FLMRIRKVPPTIIVNALIEGHKAGLKELRRDDLEAHYLAGGHVQQVVHALVSASKANI
                     DLSFQMATAIDLAGRDVFTAVQMSVNPKVIDTPPVTAVAKDGIQLIAKARVTVRANIK
                     QLVGGAGEDTVLARVGEGIVSSIGSSENHKMVLENPDSISKVVLRKGLDAGTAFEILS
                     IDIADIDVGKNIGAELQMDQAQADKNIAQAKAEERRAMAVALEQEMKAKAQEARARVI
                     EAEAEVPKAIAEAFRSGNLGIMDYYRMRNIESDTNMRESIAKPPTPKK"
     gene            complement(1411..1890)
                     /locus_tag="PKY68_08335"
     CDS             complement(1411..1890)
                     /locus_tag="PKY68_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOH25075.1"
                     /translation="MNIALVVGLIVLGIFFMILEIFFLPGVSVALIGGLACFAGGIIV
                     AYTNLGPTAGHITLISSAAAMAIAFVWFIRSKTLKKMSLETNIDSKIAAHQGAEVKVG
                     DIGRCISRLAPTGKILINGKTLEGRSENEILDEGTQVLVIRISDLNVIVKKYDDDVS"
     gene            complement(1897..>2839)
                     /locus_tag="PKY68_08340"
     CDS             complement(1897..>2839)
                     /locus_tag="PKY68_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25076.1"
                     /translation="LPLRKLFPEKGDSLSVAYLTERKDRVFYDDTTTENGWDIFSLNR
                     TMNSWETINLVKSLNQASNERFPYVLNDGITIYFASDGYNSIGGYDLYVSRYHLASES
                     FFTPEPLPMPFNSVYNDYFYIVDEYVGRGFLVTDRYAGKDSVTIYTFIPESTRKILSG
                     KSEQEVLDYARLASISLTQDGIDTDSIQTMLEERYQYDDNTVLHELEHTGSEQLRFFV
                     RDDLVYENENDFRSEEARQAYLQYLKLQARFNSLNTQLEADRPKYTGKDNPEQQELAS
                     RILNAEWVLLFLERDIPLKEYEIRRLELEKIDASINN"
BASE COUNT          722 a          650 c          665 g          802 t
ORIGIN      
        1 actatgctga ggcttttctt ttcggctttt tcgcttcgct tttcaaaatc tatattagcc
       61 agcgcatcga gttcattcat cacgatatcg atgttgtcgg tgccgatgcc ggtggaaagt
      121 acggtcattc ttttaccgta ataagatccg gtaacactgt ggaactcacg atttcgtacc
      181 cggcattcca caatatcaaa cttcgaggct accaggctta ctcgatcggg atcacctacc
      241 agtacgaccc gctcggccag ttgttccggc ttaagacgaa gatgaaaaat gcttccgtct
      301 tcgttgataa tcagctctga ttcgagaaaa tgcaaactca tggcgatgaa gtttatgccc
      361 cgtttaaaga gcagttaaac taagtgataa gggacgctat ttcttgggcg tcggaggttt
      421 ggcaatggat tccctcatgt tggtatccga ctcgatattt ctcatccggt agtaatccat
      481 tattcccagg ttgccgctac ggaaggcttc cgctattgct ttgggtactt cggcctcggc
      541 ttcaattact ctggctctgg cttcctgagc cttggctttc atttcttgtt ccagagcaac
      601 ggccatggca cgtctttctt cggccttggc ctgagcgatg ttcttatcgg cttgagcctg
      661 atccatttgc agttcagcgc cgatgttttt acctacatca atgtcggcga tatcgatcga
      721 gagtatttcg aaagcagtgc cggcatcgag gcctttgcgg agcactactt tggaaatgga
      781 atcggggttt tcgagtacca ttttgtggtt ttccgacgaa ccgatagacg atactatacc
      841 ttcaccaaca cgcgccagaa cggtgtcttc gccggcacca cccaccaact gcttgatatt
      901 tgccctcaca gtaaccctgg ctttggcaat gagctggatg ccgtctttgg caacggcagt
      961 taccggagga gtgtcgataa ctttgggatt taccgacatt tgtacggcgg taaaaacgtc
     1021 ccgcccggcc aggtcgatgg ctgtagccat ctgaaaacta agatcgatat tggctttgga
     1081 tgccgatacc agggcgtgaa ccacttgttg cacgtgacct ccggccaggt aatgtgcttc
     1141 caaatcgtcg cgccgtagtt ctttgagtcc ggctttgtgt ccctctatca gggcgtttac
     1201 aataatcgtg ggaggaactt tacgtattcg catcaaaaat agctgaatca gagaaatgtt
     1261 tactccggaa actttggccg ataaccagag gaaaaacggc acataataga agaagataat
     1321 caaaagaatg attaccggaa tcaaaataag caaaccaaat ccaggtgaca taatagtata
     1381 atttaaaagt gaatactcgt tgtttttatg ttatgataca tcgtcgtcgt attttttgac
     1441 aataacgttc aaatcggaaa tccttattac caacacctga gtgccttcat ccaatatttc
     1501 gttttcgctg cgtccctcga gagtttttcc gttgatcaga attttacccg tcggtgcgag
     1561 ccgggaaata caacggccta tgtcaccgac tttgacctct gcaccttgat gggcagctat
     1621 tttggaatcg atattggttt ccagcgacat ttttttgagc gttttggaac gaataaacca
     1681 gacaaaagct atggccatgg ccgcagcaga ggaaatcaga gtaatatggc cggccgtagg
     1741 tccgaggttg gtgtaggcca ctattatgcc cccggcaaag caagccaatc cgccaatgag
     1801 ggctacggaa acacccggaa ggaaaaaaat ctccaggatc ataaaaaaaa tacccaatac
     1861 gatcaggcca acgaccagag ctatattcat actgttttaa ttgtttattg atgcatcgat
     1921 tttttccaac tccaaacgtc ttatttcata ttccttgagc ggaatatccc gttccaaaaa
     1981 gagcaatacc cactctgcat ttaaaattct ggaagccaat tcctgttgtt ccggattgtc
     2041 tttgcccgta tacttgggcc ggtcggcctc taattgggta ttcaagctat tgaaacgggc
     2101 ttgtaacttc aaatattgga gatatgcctg acgggcttcc tcgctcctga agtcgttttc
     2161 gttttcatac acgaggtcgt ctctaacaaa aaagcgaagt tgttcactac cggtatgttc
     2221 gagctcatgc aatacggtat tgtcgtcgta ttgatagcgt tcctccagca ttgtttggat
     2281 agaatcggtg tctatgccgt cctgcgtcag agagatagag gcaaggcgtg cgtaatcgag
     2341 aacttcctgt tccgatttac cgctgaggat ttttcgggtg ctttcgggta tgaaagtgta
     2401 aattgtaaca ctgtctttgc cggcatagcg gtctgtaact aaaaagcccc taccgacata
     2461 ttcgtcaacg atatagaaat agtcgttata gacagagttg aaaggcatgg gtagaggttc
     2521 gggagtaaaa aaggattcgg aggcaaggtg ataacgactc acatacaagt cgtaaccgcc
     2581 tatggagttg tagccgtccg aagcaaaata gatggtaata ccatcgttga gcacataagg
     2641 gaagcgttcg ttacttgcct gatttaaact cttaaccagg ttgattgttt cccaactgtt
     2701 cattgtgcgg ttgaggctga aaatgtccca gccgttttcg gttgtagtgt cgtcgtaaaa
     2761 aacacggtct ttacgctctg tcaggtaggc aacactcagg gaatctcctt tttcggggaa
     2821 cagctttctg agaggaagt
//
LOCUS       DAOSGL010000296         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_33832_length_2831_cov_3.287824, whole genome shotgun sequence.
ACCESSION   DAOSGL010000296 DAOSGL010000000
VERSION     DAOSGL010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33832_length_2831_cov_3.287824"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKY68_08345"
     CDS             complement(<1..910)
                     /locus_tag="PKY68_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOH25077.1"
                     /translation="MLVPLFDYLNSIDFTGAGMFQYVSFRSIMAFIFSLLIATFIGRK
                     IILKLQRMQIGDVPRDLDVEGQLSKKGTPTMGGIIIFLSLIVPVLLFAKLDNIYIILM
                     LISTVWLTLLGFADDYIKVFKKHREGLKGKFKIIGQVGIGLIVGLTLYLSPEVVIREN
                     VQFNNGLPNEEVRFDEGEVKSTKTTIPFFKNNNLDYANLVCFMKEHKQAAGWVLFVLV
                     TIFIVTAVSNGVNLTDGLDGLTTGTSAIIGVGLGILAYVSSNMNFASYLNIMYIPGSE
                     ELVIYMSAFVGATVGFLWFNSFPAQVF"
     gene            complement(969..2423)
                     /locus_tag="PKY68_08350"
     CDS             complement(969..2423)
                     /locus_tag="PKY68_08350"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOH25078.1"
                     /translation="MKLSRCIEDLTVLEHLGPKDKEINSVCFDSRLAAPSCLFVAQKG
                     SRSDGHDYIPEVVAKGAAVIVCENLPSEINPEVTYIRTGNSNLALGLISAAWFSHPSR
                     KLKLVGVTGTNGKTTIASLLYNTLKALGYKVGLLSTVCVRVDEQELPATHTTPDAYAI
                     NSLLSQMLAAGCEYAFMEVSSHAVDQERIAGLHFAGGIYTNLTHDHLDYHKTVEAYLR
                     AKKSFFDRLPAEAFALTNADDKNGKVMLQNCRAAHYTYSCRSLADFRARLLETHSDGM
                     QLDFDGREVHVRFIGKFNVSNLLAVYGTAVLLGMDAAEVLRIISTLRPVSGRLEFLNS
                     PTGYTAIVDYAHTPDALKNVLQTLAQIRKQGKIISVVGAGGNRDKTKRPEMALEAVRA
                     SDTLILTSDNPRFEEPQAILDDMYAGVPAEVRKRVLSIVDRREAIRTACRLAGKGDYV
                     LVAGKGHEDYQEIKGVKYPFDDKKIITEIFKELL"
     gene            complement(2432..>2831)
                     /locus_tag="PKY68_08355"
     CDS             complement(2432..>2831)
                     /locus_tag="PKY68_08355"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOH25079.1"
                     /translation="QIYARELWIDLNSKRVKETKETGLPKIKTGLPGPSFYVMDKLNI
                     KYDDSGVQGERPIVVRREEESKRLLLEERNLIENLVPNVLGMGARDAILLLEQWGLQV
                     RLTGKGKVVQQSLRPGTVSREGQEIVLRLN"
BASE COUNT          764 a          722 c          605 g          740 t
ORIGIN      
        1 taaatacttg cgccggaaaa gaattgaacc acaaaaatcc cacggtggcc cccacaaaag
       61 ccgacatata aatgaccaat tcctcggaac cgggtatgta cataatattc agatacgaag
      121 caaagttcat attactcgat acataagcca atatacccaa acccactccg ataatggccg
      181 aagtccctgt agtaagacca tccaaaccat ccgtcaaatt gactccgttg gaaacggcgg
      241 ttacgataaa aatggtaacc agcacaaaca gcacccagcc ggctgcctgc ttgtgttctt
      301 tcataaaaca aaccaaattg gcataatcga ggttgttgtt tttaaaaaaa ggaatggttg
      361 tcttggtcga ttttacttca ccttcgtcaa aacggacttc ttcgttgggc aggccgttgt
      421 tgaactgaac attttcgcga atcaccactt cgggactcag ataaagggtc aaccccacaa
      481 ttaatcctat gcccacctgg ccgataatct tgaatttacc cttgagccct tctctgtgtt
      541 ttttgaacac tttgatgtaa tcgtcggcaa aacccaataa ggtaagccaa acggtactga
      601 tcagcatcaa aatgatgtaa atgttgtcca gtttggcaaa aagcaatacc gggacaataa
      661 gtgaaagaaa aatgatgatt ccacccatag taggagtgcc ttttttactt aattgaccct
      721 ccacgtccag atcgcgggga acatcgccta tctgcatacg ttgaagctta aggattattt
      781 tccggccgat aaaagtggct ataagcagcg aaaagataaa ggccataatt gacctgaacg
      841 atacgtactg aaacattcct gctcccgtaa agtcgatact gtttaaataa tcaaacaaag
      901 gcactaacat attctcttgt tttgtggctg taaactttta taaataaaac aacaaccggg
      961 tattggtatc agagtaattc tttaaaaatt tcagtaatta tttttttgtc gtcaaaagga
     1021 tatttcactc ctttgatttc ctgataatct tcgtgtcctt tgccggccac caacacgtaa
     1081 tcgcctttac cggctagtct gcaggcagta cgaatagcct ccctgcgatc gacgatggac
     1141 agcactcttt ttcgtacttc ggcaggaaca ccggcataca tatcgtccaa aatagcctgc
     1201 ggttcttcaa acctcggatt atcggaagta agtatcaggg tatcgctggc ccgcacagcc
     1261 tccagagcca tttcgggacg tttcgtttta tcgcggttgc cccccgctcc caccacactg
     1321 ataatcttgc cctgcttgcg tatctgagcc agggtttgta aaacgttctt cagagcatca
     1381 ggtgtatggg cgtaatcaac tatagcggta taaccggtgg gagagttcaa aaattccagc
     1441 ctgcccgata ccggacgcaa ggtgcttatg attctgagca cttcggcagc atccatcccc
     1501 aacaagacgg cagtcccata tacggccagt aaattcgata cattgaattt tccgataaaa
     1561 cgaacgtgta cctcccgacc gtcaaaatcc agttgcatac cgtcggaatg agtttcgagc
     1621 aatctggccc tgaaatcggc caaactgcgg caggagtagg tataatgggc agcccggcaa
     1681 ttttgaagca tcactttccc gtttttgtcg tccgcgttgg tcaaggcaaa agcctctgcc
     1741 ggtaaacggt cgaaaaagct ttttttggcc ctgagatagg cctctaccgt tttgtggtag
     1801 tccaaatggt cgtgagtgag attggtataa attcctccgg caaagtgcag accggctatc
     1861 ctttcctgat ctaccgcgtg tgaactcact tccataaaag catattcgca gccggccgcc
     1921 agcatctgcg ataacaaact gttgatggca taagcatcgg gggtggtatg agtagccggc
     1981 aattcctgtt catccacgcg aacgcaaacg gtagacaata aaccgacttt ataacccaaa
     2041 gctttgaggg tattgtagag caagctggct atggtagtct tgccgttggt accggtaact
     2101 cccaccaatt tcagcttacg cgagggatga gaaaaccagg cggcagatat taaacccaag
     2161 gccagattgc tgttgcctgt gcggatatac gtcacctccg gatttatctc cgaaggcagg
     2221 ttttcacata ctatcactgc ggcccctttt gccaccactt cgggaatata gtcgtgcccg
     2281 tcgctgcggc tgcctttttg agctacaaaa agacaagagg gagctgccag ccgggaatcg
     2341 aaacaaacgg agttgatctc cttgtctttg gggcccaaat gctccaaaac agtcaaatct
     2401 tctatgcacc gactcagttt catgcctatc gtcaattcaa acgtaatacg atttcctgcc
     2461 cttcgcgcga gacggtaccc ggacggagcg attgttgcac caccttgcct ttgcccgtaa
     2521 gcctgacttg aagcccccat tgctcgagca acaaaatggc atccctggct cccatgccca
     2581 acacgttggg caccaggttt tcaataaggt ttctttcttc cagaagcaat cttttacttt
     2641 cttcttcccg cctgaccact ataggacgct caccctgaac cccggaatcg tcgtatttta
     2701 tattcaattt gtccataacg taaaagctgg ggccgggcag gcctgtttta attttgggaa
     2761 gacccgtctc tttggtctct ttgacccgtt tactgttcag gtctatccac aattcccggg
     2821 cataaatctg c
//
LOCUS       DAOSGL010000297         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_33888_length_2827_cov_3.585859, whole genome shotgun sequence.
ACCESSION   DAOSGL010000297 DAOSGL010000000
VERSION     DAOSGL010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33888_length_2827_cov_3.585859"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="pstA"
                     /locus_tag="PKY68_08360"
     CDS             complement(<1..743)
                     /gene="pstA"
                     /locus_tag="PKY68_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOH25080.1"
                     /translation="MSSLKYRLFKNKLLTLSVVIFSALTAVPLVLIIGKVFLKGWRQI
                     NWDFFTKTTPTTLDALLAINNNQPIPGGIANGIVGTLMMVLVASVIAIPLGVMCGIYL
                     SENRKTKFSTVVRFLTDLLQGTPSIILGIIAYLWVVIPLKGYSALAGSVALAIMMLPL
                     IVRSTEETMNLLPASLKEAGLALGSPYRSVVLKVLLPSAFGGVFTGILLAVSRVIGET
                     APLMLTALGATTIQWDITKPASAVSLLIW"
     gene            complement(740..1600)
                     /gene="pstC"
                     /locus_tag="PKY68_08365"
     CDS             complement(740..1600)
                     /gene="pstC"
                     /locus_tag="PKY68_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOH25081.1"
                     /translation="MSDKIFRSLLLVSALILLLIFARMFFSLSKGAVPAFREFGFFGF
                     IFSDKWISGTGQSTEQYGALAFIAGTLMTSVLALLICLPFSLPVAFFVGEYFRGKKVA
                     GVLGGIVDLLAGIPSIVYGLWGFYALRPLIQSLGLSEQGFGVLTSSLVLAIMIIPYAA
                     SLSAEFIRMVPQELKEGAYSLGATRAEVIRNIIFPTSFSGIFSAYVLALGRAIGETMA
                     VTMLIGNTSRIPRGLMDTGNTMASIIANQFGEADGLKLSSLIAIGLLLFLITAIVNFL
                     GRLMIKRHSV"
     gene            complement(1613..2638)
                     /gene="pstS"
                     /locus_tag="PKY68_08370"
     CDS             complement(1613..2638)
                     /gene="pstS"
                     /locus_tag="PKY68_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOH25082.1"
                     /translation="MKKAIFLLAVSSLLLISCSQKTSLSGAGATFPLPYYNLVFKAFT
                     ENAGIDVSYGGIGSGGGIRSLKDEVVDFAGSDAYLSDEEMAEMKATIHVPTCLGAVVL
                     SYNLPDVSSLNLTGDIIAGIYLGKITRWNDAAIQAINPAVSLPDKAITPVYRSDGSGT
                     TFVFSDYMTKINKDWENTLGTGKSLNFAHGIAAKGNPGVAGTISQTEGAIGYIGSEYA
                     FALNSPIAYMQNAAGEFILPSSLSISAAAQEGIADDTRSMITNSSAKGSYPISCLTWI
                     IVYKDQAYANRSEDQARATLSLLNFILSPAAQAQTTMVHYAPLPKEVIDKAQKALSEI
                     TYNGKSL"
BASE COUNT          782 a          672 c          683 g          690 t
ORIGIN      
        1 tcccatatca gcaatgaaac tgccgaagcg ggcttagtga tgtcccattg tatggttgtt
       61 gctcccaaag ccgtcagcat cagcggagcg gtttcgccta taactctgga cacagccagc
      121 aggataccgg taaaaacgcc tccgaaagcc gaaggcaaaa gcactttcaa cactaccgat
      181 ctgtatgggg aacccagagc cagtccggct tctttgaggc tggccggcaa aaggttcatg
      241 gtttcttcgg tagaacggac aatcagaggt aacatcataa tagccaaagc gacactcccc
      301 gccaaagccg aatagccttt aaggggaata accacccaaa gataagcaat tatacccaaa
      361 ataatagagg gagtaccctg caataagtcg gtcaggaaac gaaccacggt cgagaatttg
      421 gtttttcggt tctcggaaag atagatgccg cacatcaccc ccaggggaat ggcaataacc
      481 gaagctacaa gcaccatcat caaggtgccc acaataccgt tggcaatacc gccgggaatg
      541 ggctggttgt tgttgatggc cagcaaggca tccagggtag tgggggtggt ttttgtgaaa
      601 aaatcccaat tgatttgtct ccagcccttg agaaagactt ttcctataat cagaaccagt
      661 ggtactgccg tgagggcaga aaatattacc accgacagcg tcagtaactt atttttgaat
      721 aatctgtatt ttaatgaact catacactgt gtcgtttaat catcaacctg cccaaaaaat
      781 taacgattgc cgttatcaaa aatagtagca ggccgatagc tataagcgaa gacaatttaa
      841 gtccgtcggc ttcgccaaat tgattggcta taatgctggc catggtgttg cctgtgtcca
      901 ttaaaccgcg gggaattctg ctggtgttac ctataagcat ggttacggcc atggtttcgc
      961 cgatggctct ccccaaggcc aaaacatagg ccgaaaagat tcccgaaaaa ctggttggaa
     1021 agataatgtt gcgtatgact tcggcccggg tagctcccag gctgtaggct ccttctttca
     1081 actcctgggg aaccatacga ataaattcgg cgctcagcga agctgcataa ggaataatca
     1141 tgatagccag caccagcgat gaggtcaata cgccaaaacc ctgttccgat aggcccaggg
     1201 actggatcaa aggcctcagg gcataaaagc cccataagcc gtacactatc gaaggaatgc
     1261 cggccagcag atcgactatg ccgcccaaaa cacccgccac ttttttcccc ctgaagtatt
     1321 ctcctacaaa aaaagctaca ggcaaggaaa agggtaagca aattaacagg gccagcacag
     1381 aggtcattaa ggtccccgca atgaaagcca gggcaccgta ttgttcagtg ctctggcccg
     1441 ttccggaaat ccacttatcc gaaaaaataa agccaaaaaa tccgaattct ctgaaggcag
     1501 gaaccgctcc tttgctcaaa gaaaaaaaca tacgcgcaaa gatcagcaag aggatcaaag
     1561 ctgagaccag gagcaaggat ctgaagattt tgtccgacat cagcagccca aattacagag
     1621 acttaccgtt gtaagtaatc tccgacaagg ccttttgtgc tttgtcaatg acctctttcg
     1681 ggagcggagc ataatgtacc atggtagttt gcgcttgtgc tgccgggcta agtataaagt
     1741 tcagcaggct gagcgtggcc cgggcctgat cttcgctcct attggcgtaa gcctgatctt
     1801 tgtacacaat tatccacgta aggcaactga tagggtagga gccttttgcg ctggaattgg
     1861 ttatcatgct gcgagtatcg tcggcgattc cctcctgagc tgcggccgag atagaaaggc
     1921 tcgaaggcaa aataaattct ccggctgcgt tttgcatata agcgatgggg ctgttaagcg
     1981 caaaggcata ttcagaacct atgtaaccta tggcgccttc ggtctggctg atggtgccgg
     2041 ccactccggg attacctttg gcggctatgc catgggcaaa attgagcgat ttgcctgtgc
     2101 cgagagtatt ttcccagtct ttgtttattt tggtcatata gtcgctgaac acaaaagtgg
     2161 taccgctgcc gtcggagcga tatacaggag taatcgcttt atcgggtaaa cttactgcag
     2221 gattgattgc ttggatggcg gcatcgttcc agcgagtgat tttacccaag taaattccgg
     2281 cgatgatatc tccggtcaga ttcagggaac ttacatccgg caggttgtac gaaagtacaa
     2341 cggctcccag gcaggtggga acgtgaatgg tggctttcat ctccgccatc tcctcatcgc
     2401 tgagataagc gtcgctgcct gcaaaatcga ccacttcgtc cttaaggctg cgtatgcccc
     2461 cgccgcttcc tatgcctccg taagagacat cgatgcctgc gttttcggta aaagctttaa
     2521 agactaagtt gtaatagggc agggggaaag tggctcctgc tccggacaaa ctggttttct
     2581 gactacaact gatcaacagt aaactgctga cagccaaaag gaaaattgct tttttcattt
     2641 tcttcgttat tgattaaatt gaaaggttta atttttacgc gaaattcaac aaagcatatg
     2701 tcagacctgt ttcaaacttg ttacgatcct gttaagatgt taagtttcgc tcgggttcaa
     2761 tcgcgtgaat ataccagacc attttggagt ttatattgta taggtccgta attcttagcc
     2821 agataga
//
LOCUS       DAOSGL010000298         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_33975_length_2822_cov_3.923021, whole genome shotgun sequence.
ACCESSION   DAOSGL010000298 DAOSGL010000000
VERSION     DAOSGL010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33975_length_2822_cov_3.923021"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKY68_08375"
     CDS             <1..331
                     /locus_tag="PKY68_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25083.1"
                     /translation="EKWEAFGWYVVEEDGHDIGRLLPVLERAFQRGRATREERQGMSL
                     LPDHLRRGRASAGVPHPDQPVVLIAHTLKGKGVPYMEGDNSWHKRVPTDEEHQLALSI
                     LGGEDLE"
     gene            324..1292
                     /locus_tag="PKY68_08380"
     CDS             324..1292
                     /locus_tag="PKY68_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOH25084.1"
                     /translation="MNNVSSTRVGFMQGLEALAEEDDRIMLVCADSLKAMRADPFLEK
                     FPDRVVELGIAEQNAAGVSAGLALGGLIPYFATYAGFITMRACEQIRTFIAYPNLDVK
                     MVGINGGLAAGEREGVSHQFFEDLAIMRSIPNITVLVPADAGQARQAAKAAAEIPGPV
                     YIRLGSGRDPVIFDDKQPFVPGKIHILEQRGNDLVFFCNGLMIPRVLVASALLEAKGI
                     ACTVVEVHTLKPLDTEAIVELLKDCRAAVTAEDHNIIGGLGSAIMECAAEHCPVPVAR
                     IGLRDVFPGSGHPEALLDFYGMSVNDIVEAAVSTINRKPNPLPNEE"
     gene            1294..2688
                     /locus_tag="PKY68_08385"
     CDS             1294..2688
                     /locus_tag="PKY68_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOH25085.1"
                     /translation="MQTGYFKKIKEQTATRLWINNPTIGDVRHGLEAGAIHCTTNPTY
                     AARMLREAESRDFALGVIDTVLAACPDDDKAAAIIQRRLVAEMAKGFMPHFEATKSKE
                     GLVSIQMDPRFEEDAAAIVAEALADFALIPNAIAKIPVIPSGLVAIDCLVRLNKPVIA
                     TEIMGIAQALAACEVYKRASEESGFSPAFFVTHISGILDEELKAEAEALGVEVSSEAM
                     YIAGCAVAKKQYRLMKERNLPGILLGGGARGTRHFTEFVGGDLHITLNWSGTALELEQ
                     NPPALESRIDAPVPQKLIDELKTKLPTFARAYDEKGLKPEEFHHFAPVVRFRNQFLKG
                     WDALLATIRERRALRVNVQRSYCKLGEHKPVEIRPGIFRSTLVYNAENMLCHFFEKEG
                     ARVDLHTHHAIQSGYVLRGKLLFFDAEGNEHILLPGHGYLFGSNEPHGSVALEESEII
                     EIFTPGRPEYLDKE"
BASE COUNT          668 a          695 c          797 g          662 t
ORIGIN      
        1 cgaaaaatgg gaagcctttg gctggtatgt ggttgaagaa gacggacacg atatcggccg
       61 gcttttgccc gtgctcgaaa gagcctttca acggggcaga gcaacgcgcg aagaacgtca
      121 gggaatgagc ctgttgcccg atcacctcag gcgggggcgg gcttcggcgg gcgtgccgca
      181 tcccgatcag cccgtggtgc tgattgcaca cactctgaag ggaaagggtg ttccctatat
      241 ggaaggcgat aattcctggc ataaacgggt gcctaccgac gaagaacatc aattggcttt
      301 atcaattttg ggaggagaag atcttgaata atgtatcgag tacccgtgtg ggttttatgc
      361 agggcctgga ggctctggca gaagaagacg acagaataat gctggtttgt gccgacagcc
      421 tcaaggctat gcgtgcagac ccttttctgg agaaatttcc cgacagggtg gtggagctgg
      481 gcatagcgga gcaaaatgcg gccggtgtat cggccggatt ggccctgggc ggattgattc
      541 cctattttgc cacttatgcg ggctttatca ccatgcgggc ctgtgagcag atccgtactt
      601 ttattgccta tcccaacctg gatgtgaaga tggtgggaat caacggcggt ctggcggccg
      661 gcgaacgcga aggggtgagt catcagtttt ttgaagattt ggccattatg cgcagcattc
      721 ccaatataac cgttctggtg ccggccgatg ccggtcaggc ccgtcaggcg gcaaaggcgg
      781 cagccgaaat tcccgggccg gtgtacatcc gtctgggcag cggtcgcgat ccggtgattt
      841 ttgacgataa acagcccttt gtgccgggca agatccacat cctggaacaa agaggcaacg
      901 atttggtctt tttttgcaac ggcctgatga ttccccgggt gctggtagct tcggctttat
      961 tggaagccaa aggcatagcc tgtaccgtgg tcgaagtgca tacccttaaa cccctggata
     1021 cggaggctat agttgaactt ttgaaagatt gccgggcagc ggtaacggcc gaagaccaca
     1081 acataatagg cggtctgggt tcggccatca tggagtgtgc tgccgagcat tgcccagtgc
     1141 ctgtggcccg catcggtttg cgcgacgtct ttccgggttc gggtcatccc gaggccctgc
     1201 tcgattttta cggcatgagt gtgaacgaca tagtggaggc tgccgtcagc accatcaaca
     1261 gaaaacccaa tcctttacca aacgaagaat aatatgcaaa caggttattt taagaaaata
     1321 aaagaacaaa cagctacccg gctgtggatc aataatccga ccatcgggga tgtgcgccac
     1381 gggttggaag caggagccat tcattgtacc accaatccta cgtatgctgc ccgtatgctg
     1441 cgcgaggccg aaagccggga ttttgccctg ggggtgatag acactgtgtt ggccgcttgt
     1501 cccgacgacg ataaagccgc tgcgattata caacgccggc tggtcgcgga gatggccaaa
     1561 ggctttatgc cgcatttcga agccaccaaa agcaaagaag gtttggtgtc catacaaatg
     1621 gatccccgtt tcgaagaaga tgctgcggct attgtggccg aagcccttgc cgattttgct
     1681 cttattccca acgctatagc caaaattccc gtgattcctt cgggtctggt ggccatcgat
     1741 tgcctggtca ggctgaacaa accggtgatc gccaccgaaa ttatgggcat agctcaggca
     1801 ctggctgcct gcgaggtata taaacgggct tccgaagaat cgggtttttc tcccgccttt
     1861 tttgtgaccc atatttcggg catccttgac gaggaactca aggccgaggc cgaagctctg
     1921 ggcgtggaag tcagtagcga agcgatgtac attgccggct gtgccgtagc caaaaaacaa
     1981 taccgcctga tgaaggagcg aaacttaccg ggtattttac tcggcggagg tgcccgtggc
     2041 acccggcact ttaccgaatt tgtaggcgga gatttgcata taacgctcaa ctggtccgga
     2101 acagctctgg agttggagca gaaccctccg gctttggaat cgcgcatcga tgcgccggta
     2161 ccacagaaac ttattgatga actcaaaact aagttgccga cctttgcccg tgcttacgac
     2221 gaaaagggat taaagcccga agaatttcac catttcgcgc cggtggttcg ttttcgcaat
     2281 cagttcctga agggctggga cgcattgctg gcgaccattc gcgaacgccg ggctctcagg
     2341 gttaacgtgc aacgctccta ttgtaaactg ggtgaacaca agcccgtaga gatacgtccg
     2401 ggcatttttc gttctacctt ggtttacaac gccgaaaaca tgctttgtca ctttttcgaa
     2461 aaagaaggcg ctcgtgtaga cctgcatacg caccacgcca ttcagagcgg ctatgtgctg
     2521 cggggtaagt tgctgttttt tgatgccgag ggcaatgaac atattctgtt gcccgggcat
     2581 ggctatttgt tcggttccaa cgagccccac ggttcggtgg cactggagga atcggaaatc
     2641 atcgagatct ttacgccggg aaggccggaa tatttggata aagaataatt cgatacatca
     2701 attcactaaa aaataaaaca catatgacca gagtttttgc cgttcatacg gccactgcat
     2761 taattgagcc tattacggct ttgtttaaag aacacctgcc tcaggtgaag ttgaaccaca
     2821 tt
//
LOCUS       DAOSGL010000299         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_34270_length_2806_cov_3.411487, whole genome shotgun sequence.
ACCESSION   DAOSGL010000299 DAOSGL010000000
VERSION     DAOSGL010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34270_length_2806_cov_3.411487"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKY68_08390"
     CDS             complement(<1..226)
                     /locus_tag="PKY68_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOH25086.1"
                     /translation="MLNIVIFGGPGSGKGTQSALIAEKYNLIHLSTGDLLRDEAASGS
                     ELGKLIHSFIDKGNLVPDQLMLDILSRTIDN"
     gene            complement(271..810)
                     /gene="hpt"
                     /locus_tag="PKY68_08395"
     CDS             complement(271..810)
                     /gene="hpt"
                     /locus_tag="PKY68_08395"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOH25087.1"
                     /translation="MKTVQVKDRSFALLLTAERIRKRVSELALEINRDMEGKNPLFLV
                     MLNGAFMFAADLLKEINVPCEISFIKYDSYRGTKSSGRLNKLIGLKESLRNRCVIVLE
                     DVVDSGLTMSRLFEELKTKKPAELYLATCIFKPEALKSEVKPDYVGFNLPDVFLVGYG
                     LDYEGYGRNLEAIYELKSR"
     gene            946..2478
                     /locus_tag="PKY68_08400"
     CDS             946..2478
                     /locus_tag="PKY68_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOH25088.1"
                     /translation="MALNIYTTAQIRQLDADTIQIEGIDSSELMERAALGLFERIKIL
                     IAPNKSILALAGPGNNGGDALALSRLMLEAGFQLRCLLLSGNKTLSHDCALNAKKLIE
                     LFPSSFVFDATPEDLIKEAPTDYLIDGLFGSGLNKALEGDFARIVHWLNKQKALRISI
                     DLPSGLHGEDNRTNKPENIVRADLVLGIQFPRLAFLLPENEDYIGQWELTPLDFHPEA
                     LARQTTNYSLVEQNDIRAVLRNRKKFSHKGNYGHGLLIAGSAGMPGAAILAGRAALRA
                     GLGLLSIRMPSALSACIQSAVPEAMTSIDPLSENNISSMENLDLDRFSAIAIGPGLGT
                     GQGQTRVMKALLKETKKPLVIDADGLNILAAEPSLLSLLPRGSILTPHPGEFDRLAGA
                     SDCGYERLEKASAFAKKHGVYLILKGHYTCSIFPDGSRHFNNTGNSGMATAGSGDVLC
                     GILLSLLVQAYPPAQCCLLGVYLHGLSGDLALKEQSEESLVAGDLIQYLGKAFKHSKG
                     QI"
     gene            2491..>2806
                     /locus_tag="PKY68_08405"
     CDS             2491..>2806
                     /locus_tag="PKY68_08405"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOH25089.1"
                     /translation="MKKLLFLSLVVLGLSGNLTAQIQSRIVEGPIPYEAFSYHLPKTV
                     FKVRVTIRTTHEEPGIYARYSERLLGISDIVTTERVNNELVSVELDTYTLPDPKRVFY
                     IQA"
BASE COUNT          795 a          663 c          594 g          754 t
ORIGIN      
        1 aattatcgat ggttcttgac aaaatatcta acatcaattg atcgggtacc aaattgcctt
       61 tgtcaataaa agagtggatt aatttgccca actccgaccc gcttgctgct tcgtcacgca
      121 aaagatctcc cgtggacagg tgtatcagat tatatttttc ggctattaat gcactctggg
      181 tcccttttcc cgatcccgga cctccaaaaa ttacaatgtt cagcattttc tttaaaaatt
      241 atcctctata tgccacctaa taatctcaac ttagcgggac ttcaactcat aaatggcttc
      301 taaatttctt ccgtaacctt cataatccaa accgtagcca accaaaaaaa catccggtaa
      361 attgaagccc acataatccg gcttaacctc agacttcaat gcctccggtt taaagatgca
      421 ggtggccagg tataattcag cgggtttctt tgttttcaat tcttcaaaca accggctcat
      481 agtcaaaccg ctgtctacaa catcttccaa aacgattaca catcggttac gtaacgactc
      541 tttgagccca atcagcttgt taagccttcc gctgcttttc gttccacgat aggaatcgta
      601 tttaataaaa cttatttcgc agggaacatt aatttctttc agtaaatccg ctgcaaacat
      661 aaaagctccg ttgagcatta ctaaaaacag cggattctta ccctccatat ctctgtttat
      721 ttccagtgcc aattcggaaa cccttttccg gatacgttcg gcagtaagaa gtaaagcaaa
      781 gctccggtct tttacctgaa ctgttttcat gagtttcaga aagaatacaa cgaagttgcg
      841 aatatacaaa aaaatggctg tctggaaatt gatcgggaat tattttttac ttttgtatta
      901 aatttgtaca gatttttgga taaatatacg gataatcgta acaaaatggc ccttaatata
      961 tacaccacag ctcaaatcag acaactggat gccgacacaa ttcaaatcga aggtatcgat
     1021 tcttccgaac taatggaaag agctgcacta ggcctttttg agcgaatcaa aatactgatt
     1081 gcccccaata aaagtatact ggctctggcc gggccgggaa acaacggagg agatgccctg
     1141 gctttatccc gtttgatgct ggaggcgggg tttcaactca gatgtctttt attgagcgga
     1201 aacaaaacac tttcgcacga ttgcgctttg aacgcaaaaa aactgattga gttgttccct
     1261 tcatcattcg tatttgatgc cacgccggaa gatttgataa aagaggctcc cacggattac
     1321 cttatagacg gattgttcgg ctccggacta aacaaagcgc tggaagggga ttttgcccgg
     1381 attgtacatt ggctgaataa acaaaaagcc ctgcgtattt ccatagattt gccttcgggc
     1441 ctgcatggag aagacaaccg gacaaacaaa cccgaaaata tagtacgcgc agatctggtg
     1501 ctgggtatcc aattcccgcg actggctttt ttactgcccg aaaacgaaga ttatataggc
     1561 cagtgggagc tgactccgct ggacttccat cccgaagctc ttgcccgtca aacaacaaat
     1621 tattcgctgg tagaacaaaa cgacataaga gccgttttgc gaaatagaaa aaaattcagc
     1681 cacaaaggga attacggaca cggtctactg attgccggca gcgccggaat gcccggagcg
     1741 gctattctgg cagggcgcgc ggccttgagg gcaggacttg gcctgctgag catcagaatg
     1801 ccttcggcct tgtctgcttg cattcaaagc gccgttccgg aagccatgac aagtatcgat
     1861 ccgctttccg aaaacaatat ttcctccatg gaaaatttgg atctcgaccg tttttctgcc
     1921 attgccatag gcccgggcct gggaacggga cagggtcaaa ctcgggtcat gaaggccttg
     1981 ttgaaagaaa caaaaaaacc tttggtcatt gatgcagacg gtctcaatat tctcgctgcc
     2041 gaaccttccc tattaagctt attgcctcgg ggatccatct taactcctca ccccggggag
     2101 tttgaccggc tggccggagc ttcggattgc gggtatgaac gtctcgaaaa agctagcgct
     2161 tttgctaaaa aacacggggt ataccttatt ctgaaagggc attacacgtg tagcatcttc
     2221 ccggacggca gtcgtcattt caacaacacc ggcaattccg gaatggcaac ggcaggcagt
     2281 ggcgacgttc tgtgcggaat tttattatct ttgctcgtcc aggcttaccc tccggctcaa
     2341 tgctgcctgc ttggtgtata tctgcatggc ctgagtggag acctggccct gaaagagcaa
     2401 tcggaagaaa gccttgtagc cggagacctg attcagtatc ttggtaaagc cttcaaacat
     2461 agtaaaggac aaatttgaac ttatatattt atgaaaaaac tgctgttttt atcgctcgtt
     2521 gtactcggtt tatccggaaa ccttactgca cagattcaaa gtcgtattgt agaaggtccc
     2581 atcccttacg aagctttcag ctatcatctt ccaaaaactg tgttcaaggt tcgtgtcacg
     2641 atcagaacca cacacgaaga gcccggcatt tatgccagat attccgaaag attgcttggg
     2701 atcagcgata ttgtaactac agaaagagta aacaacgaac tggtatcggt ggaattagat
     2761 acttataccc tgcccgaccc taaaagagtt ttttacatac aagctg
//
LOCUS       DAOSGL010000300         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_34522_length_2791_cov_4.134503, whole genome shotgun sequence.
ACCESSION   DAOSGL010000300 DAOSGL010000000
VERSION     DAOSGL010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34522_length_2791_cov_4.134503"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PKY68_08410"
     CDS             <1..982
                     /locus_tag="PKY68_08410"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOH25090.1"
                     /translation="TSLSYTRHPDSNHLQQIVSEQLTASGFNEVINNSLVSSAHYEGL
                     STFPPKACVRLLNPLSKDLNVMRQSLLFGGLQNLSYNINRKNPDLRLYEFGNCYQYRD
                     EKKAAGNNLAAYDESMHLGLWLCGNRNSIHWMQPASALSFYDLKAYVHHILLRLGLEP
                     EKLEISEISPGDIFTQALCYSSKAGKTLLTMGIVNAAILQAFDLDTEVYYADIHWDNL
                     LKALKTNEISFKELPKFPEVRRDLALLLDHSVSFSMIENIARVSEKQLLKSLYLFDVY
                     EGKNLPAGKKSYAVTFVLRDENQTLTDERIDACMQKIIRNLQSQLGASLR"
     gene            995..1729
                     /locus_tag="PKY68_08415"
     CDS             995..1729
                     /locus_tag="PKY68_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH25091.1"
                     /translation="MGRAFEYRKARKLKRWGNMARTFTKIGKEISSCVKESGPDPDSN
                     PRLRVLIQNAKAANMPKENVERAIKKAVSKDYVDFKEMVYEGYGPFGIAILVETQTDN
                     TTRTVASVRSAFNRYGGSLGTTGSLSFMFERKAVFKIKPKEDLDPDELELELIDYGVD
                     ELETEDEDIVIYGEPESFARIQKYLEENEFEIVSAEFERFPNETKELTPEQEATINKL
                     IERLEEDEDVSNVYHNMKESLSEEDA"
     gene            complement(1726..>2791)
                     /locus_tag="PKY68_08420"
     CDS             complement(1726..>2791)
                     /locus_tag="PKY68_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOH25092.1"
                     /translation="PHLKDFRERIRINGRCISETYVSEFTTAYVDAKKAGLILEADFK
                     PSFFELTTTMAFSYFAENNVDVAVIEVGLGGRLDSTNIIDPVLALITSISLDHMSLLG
                     NNVLSIAGEKAGIIKSGRPAVVGINPSEVYRVLRKRALQEQAPFYLAEDLLQLQGETF
                     NKGTKLKVKSGVWQELNTFTCGLAGQYIPENATLVLAALSLLKQDFIQINAPALLRGF
                     EQVVPNTGIRGRWEILQEKPLCICDTGHNEAGIRLVTKQLAATPHKQLHIVLGVVNDK
                     DIGAMLELLPQKALYYFTKAAVPRALPETELAALAAARNLKGQSYPTVAEAKAAALKA
                     AAADDLIFIGGSNFIVAEAL"
BASE COUNT          744 a          686 c          673 g          688 t
ORIGIN      
        1 aacctcgctc tcttataccc ggcaccccga cagtaaccac ttgcagcaga ttgtttcgga
       61 acaattgacc gcatcgggct tcaacgaagt gatcaacaat tcgctggtgt cgtcggctca
      121 ttacgaagga ttgagcactt ttcccccgaa agcctgtgtg cgcctgctga atcctctgag
      181 caaagacttg aacgtaatga gacaaagcct cttgtttggc ggcttgcaaa acctctcata
      241 caacatcaac cgtaaaaatc cggatttgcg cttgtatgaa ttcggcaatt gttatcaata
      301 ccgcgacgaa aagaaagcag ccggcaacaa cctggccgca tacgacgaaa gcatgcacct
      361 ggggctgtgg ctctgcggta accgcaattc cattcattgg atgcagcctg cctcagctct
      421 gagtttctac gacctcaaag cttatgtgca ccatattttg ttacgcctgg gtttggaacc
      481 cgaaaaactg gaaataagcg aaatcagccc gggggatatc tttacacaag ccctttgcta
      541 cagcagcaaa gccgggaaga ccctgcttac gatgggtata gtaaatgctg cgatactgca
      601 agccttcgac ctcgatacgg aggtgtatta cgccgatata cattgggaca atctgctcaa
      661 ggccctgaaa acgaatgaga tttcgttcaa ggaattacct aaattccccg aagtccgccg
      721 ggatttggcc ttgttgctcg accattcggt gtctttttcg atgatagaaa acatcgccag
      781 agtttccgaa aagcaattac tgaaatcgct ttatttgttt gatgtgtacg aaggaaagaa
      841 cctgcctgcg ggcaaaaaat cctacgccgt gacttttgtt ttaagggacg aaaaccaaac
      901 actcaccgat gaacgcatcg atgcctgcat gcaaaaaatc atccggaatc tacaatcgca
      961 attgggcgcg agcctgagat aaaatacctg ccttatggga agagcctttg aatacagaaa
     1021 agccagaaaa ttgaaacgtt ggggcaatat ggcccgtact tttaccaaaa tcggaaaaga
     1081 aatatcgagt tgcgtcaaag aatccggccc tgatcccgac tccaacccgc gtctgagggt
     1141 tttgatccaa aacgccaaag cggccaatat gcccaaagaa aacgtggaaa gggctatcaa
     1201 gaaagctgtt tccaaagact atgtcgattt caaggaaatg gtttacgaag gttatggtcc
     1261 ctttggtatc gctattttgg tcgaaactca gaccgacaac accacccgta cggtagcttc
     1321 ggttcgcagt gcctttaatc gttacggagg ctccctgggt acaacgggca gcctttcctt
     1381 tatgttcgag cgcaaggcgg tcttcaaaat aaaacccaaa gaagacctcg acccggacga
     1441 attggaactg gaattaatcg actacggtgt ggatgaactt gaaacagaag acgaagacat
     1501 cgttatctac ggcgaacctg aatcctttgc ccgcattcaa aagtacctcg aagaaaatga
     1561 atttgaaatt gtgtcggccg aatttgaacg ttttcccaac gagaccaaag aattgacacc
     1621 cgaacaggag gccaccatca ataaattgat cgagaggctc gaagaagacg aagacgtctc
     1681 caacgtttat cacaacatga aagaaagcct ttcggaagaa gacgcttaaa gagcttcggc
     1741 aactatgaag ttgctgcctc ctataaaaat caaatcgtct gcagcggctg ctttcaaagc
     1801 ggccgctttt gcttctgcca ccgtgggata gctctgtcct tttaagtttc gcgctgccgc
     1861 caacgcagcc agttccgttt cgggcaaggc tctgggtacg gctgctttgg taaaataata
     1921 aagagccttt tggggcagaa gttccagcat ggctccgatg tctttgtcgt tgaccacgcc
     1981 caatactata tgcagttgtt tgtggggtgt ggcggccaat tgcttggtta ccaaccgtat
     2041 tccggcttca ttgtgtcctg tatcgcaaat gcataaaggc ttttcctgaa ggatttccca
     2101 acggcctctg atgcctgtgt tgggtaccac ctgttcgaat cctctgagca gggcaggggc
     2161 gttgatttga ataaagtctt gcttcaacag actaagggcg gccagtacca aagtggcgtt
     2221 ttcgggaatg tattggccgg ccagcccgca ggtaaaagtg ttcaattcct gccagactcc
     2281 ggattttact ttaagctttg ttcccttatt gaacgtctct ccctgcaatt gcagcagatc
     2341 ttcggccagg taaaagggag cttgctcttg cagagcgcgc ttgcgtaaca cccggtacac
     2401 ttcggaggga tttattccca ccactgccgg cctgccagat ttaatgatgc ccgctttttc
     2461 gcctgcgatg ctaaggacat tgttgcccag caggctcatg tgatcgaggc tgatactggt
     2521 gataagcgcc agaacggggt ctataatatt ggtactgtcg aggcgtccgc ccaggccgac
     2581 ctcaatgacg gccacatcta cgttgttttc ggcaaaataa ctgaaggcca tggtggtggt
     2641 aagttcaaaa aacgaaggct tgaaatctgc ctctaagatt agcccggcct ttttggcatc
     2701 gacgtaggct gtggtaaatt cgctgacata agtttccgaa atacatcgcc cgttgatgcg
     2761 gatacgctcc ctgaagtctt ttaaatgggg c
//
LOCUS       DAOSGL010000301         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_34542_length_2790_cov_3.798172, whole genome shotgun sequence.
ACCESSION   DAOSGL010000301 DAOSGL010000000
VERSION     DAOSGL010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34542_length_2790_cov_3.798172"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKY68_08425"
     CDS             complement(<1..443)
                     /locus_tag="PKY68_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25093.1"
                     /translation="MNKSRRYLLGFLILALMQAVQAYAQRISFGTYATDDIVLTALGT
                     GELNFNNKQPVIKSGDVITINLTDNSTAVLSVTGNRHLDVTLTVEAPTVLELDASNTI
                     PLVLRFAYSNQGLAESSAKAAAVEVPLGFNSATFPLQRRAPGTPPP"
     gene            complement(453..968)
                     /locus_tag="PKY68_08430"
     CDS             complement(453..968)
                     /locus_tag="PKY68_08430"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOH25094.1"
                     /translation="MSKKIQILLIVFMLLAAGRPVEAASPTSASVSIQASATVMDKSE
                     IELITVKNMEINASMAEEGRIYISAQRNPHAGMMMIKGKAGSSFRISFLPVAEITNTV
                     GKGSLFFQYEVYGYENDNQGASEPIDAAERILKMNKEGLYYFWIGGRIDIRKARPGSY
                     SGEFTIEVEYI"
     gene            complement(1076..1882)
                     /locus_tag="PKY68_08435"
     CDS             complement(1076..1882)
                     /locus_tag="PKY68_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH25095.1"
                     /translation="MKLQAQDLNTTSYLSFGDPAALGIDYWDAFRLEPMSETWLELFS
                     LSPENPKIPLVINNLPLPSGEQGMNISLFVDGQLRGQGLSGSYILSWEIPAAWPPDWE
                     LSLHDHSRQLAVSMHEQSSYSFEHRSSVTRALKDSRPLIYKAPRPITRIDPGSLTRSS
                     TELPPYTIRVSKAGKALEYIAPQPKLLTNYPNPFTGVSRVRFSLPKADKAALRVYNAQ
                     GLLVALLADAEFPEGISELEWNAGALPAGLYFIRFSSGGVLQTNKAIIKN"
     gene            complement(1896..>2790)
                     /locus_tag="PKY68_08440"
     CDS             complement(1896..>2790)
                     /locus_tag="PKY68_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25096.1"
                     /translation="KDNATLSLQAGPLLSLQPGITVNTNNSGKIVLESGARYLNLSTA
                     TPRLEVRRELSGGKGWRMLSSPVPVTFAQWLPSPLVTQGYTGSTFPTLQPNLLWWDET
                     DAGTTLQGWRQPANSGDSIVKGRGYFYFIFNGAGRLDSAGNSTGQNYTDVLPKTLSVT
                     GVEAFGGSGVFSYNLHFTPRDSSSQNPLLDTIFYDITRYDQGWNLLGNPTASTLDWDS
                     SWTKVAVSNAIYIWDPSANSGNGDYLTWNGSTGSLGSGRIAPFQAFWVHTSSSPQLSF
                     GNAAKSSVSGSFFAIGRGERY"
BASE COUNT          668 a          697 c          739 g          686 t
ORIGIN      
        1 gggggcggcg taccgggagc ccttcgctgc aagggaaaag tggccgagtt gaatcccaga
       61 ggcacttcta cagcggcggc tttggcggag gattcggcca ggccctggtt ggaataagca
      121 aaacgcagaa ccaggggtat agtgttggag gcatccaact ctaagaccgt aggggcttcg
      181 acggtgagtg taacgtccaa atgacggttg cctgtgacac ttaaaacagc ggtggagttg
      241 tcggtcaagt tgatggtaat tacatcgccc gacttgatca ccggctgttt gttgttgaaa
      301 ttgagttccc ctgtccccaa agctgtcaat acaatatcgt ccgtggcata ggtcccgaag
      361 ctgatccgct gggcataggc ctgtacagcc tgcatcaaag caaggatcaa gaaaccgagt
      421 aaatagcgcc tggacttgtt catcttttat gcttaaatat attccacttc tatggtaaat
      481 tctccgctgt aactgccggg gcgggccttt ctgatgtcga tgcggccgcc tatccaaaaa
      541 taatacagcc cttccttgtt catttttaaa atacgctctg cagcatcgat gggctcgctg
      601 gctccctgat tgtcgttttc gtagccgtaa acctcgtatt ggaaaaacaa agagccttta
      661 cctaccgtgt tggtaatttc ggccaccggc aaaaaactga tacgaaaact cgacccggct
      721 ttccctttga tcatcatcat gccggcatgg ggattgcgtt gagccgaaat ataaatgcgt
      781 ccctcttcgg ccatggaggc attgatctcc atatttttga cggttatcaa ttctatttcc
      841 gatttgtcca tcaccgtagc cgaagcctgt atgctaacgg aagcagaggt gggggaagcc
      901 gcctctacgg gcctgccggc tgcaagcagc ataaaaacaa tcaatagtat ttggattttc
      961 ttagacatgg taaatctaga tattgtttat acaccctgaa caaaggcaat tagtgtgcca
     1021 ttttttttta tttgtttatt ccggtcttaa tctttgtttt tattatgtta agtctctaat
     1081 ttttaatgat ggccttattc gtttgcaaca cccctccgga cgaaaagcga ataaagtaca
     1141 aaccggcagg caaagcaccg gcattccatt ccaattccga gatcccctcc ggaaattcgg
     1201 catcggcgag caaggccacc aacagcccct gagcattata aaccctcaga gcagctttgt
     1261 cggccttggg caggctaaaa cgtacacggg ataccccggt gaaaggattg gggtaattcg
     1321 tgagcaactt aggttgagga gctatgtatt ccaaggcttt tcccgctttg gatacacgta
     1381 tggtatatgg aggcaactcg gtgctgctgc gcgtcaggct gcccgggtct atacgggtta
     1441 tcggccgggg ggctttgtaa atcaagggcc ggctgtcttt aagtgctcgg gtaacggaac
     1501 tgcgatgttc aaagctgtat gaactttgtt cgtgcatcga tacggccagt tgccggctgt
     1561 gatcgtgcag gctcagttcc caatccggag gccaggcggc cggaatttcc cagctcaata
     1621 tatagcttcc gctcaaaccc tgcccccgga gctgtccgtc gacaaagagg gaaatattca
     1681 ttccctgctc acccgaaggc agaggcaaat tattgattac caatggtatt tttgggtttt
     1741 cggggctcag actgaataat tccaaccagg tctcgctcat gggttccagg cggaaggcat
     1801 cccaataatc gatacccaaa gcagccggat cgccaaaaga gagataagaa gtggtattca
     1861 aatcctgggc ttgtagtttc aaagccaccg acaacttagt atctttcgcc ccggccgatc
     1921 gcaaaaaaac taccggatac ggagcttttg gcagcattgc caaaactcag ttgagggctg
     1981 gaagaggtat gcacccagaa ggcctggaag ggtgcaatcc ttccactacc caggctgccg
     2041 gtgctgccgt tccaggttaa ataatcgccg ttgccgctgt tggccgaggg atcccaaata
     2101 tatatagcgt tacttacggc cactttggtc cagcttgaat cccagtcgag ggtgctggcc
     2161 gtgggattgc ccaggagatt ccatccctga tcatagcggg tgatatcgta aaaaatcgta
     2221 tccaacaggg gattctggct ggaactgtcg cggggcgtaa aatggaggtt gtagctgaaa
     2281 acgccggaac cgccgaaggc ttcgacacct gtcacagaga gagtctttgg cagtacatcg
     2341 gtataattct ggccggtaga gtttccggct gaatcgagcc gcccggcgcc attaaaaata
     2401 aagtaaaaat agcctctgcc cttcacaata ctgtcgccac tgttggcggg ttgtctccag
     2461 ccctggagag tggttcccgc gtcggtttcg tcccaccaca acagattggg ttgcagcgtg
     2521 ggaaaggtcg atccggtgta gccctgggtg accaagggcg agggcaacca ttgagcaaaa
     2581 gtgaccggca ccggcgagga aagcatgcgc caaccctttc cgccgctgag ctcgcgccgt
     2641 acttcgagcc gaggagtagc ggtgcttaaa ttcaggtagc gggcacccga ttccaaaacg
     2701 atttttccgc tgttgttggt atttaccgtg atgcccggct gcaagctgag cagcgggccc
     2761 gcctgcaaac ttaaggtggc gttatctttg
//
LOCUS       DAOSGL010000302         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_34600_length_2787_cov_3.674963, whole genome shotgun sequence.
ACCESSION   DAOSGL010000302 DAOSGL010000000
VERSION     DAOSGL010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34600_length_2787_cov_3.674963"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PKY68_08445"
     CDS             complement(<1..705)
                     /locus_tag="PKY68_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOH25097.1"
                     /translation="MNRRNFLKIFALTGVGLAVKPAKSLAALVPEENKTEQAVDLVAV
                     MGGEPDQMFRRAIAEFGGMNQFVKSGQKVCIKPNIGWDRVPERAGNTNPLLISEIIRQ
                     CFAAGASEVTVFDHTCDDWVKCYQNSGIEAAAKEAGAKVLPGHQESYYRQVELPKGKV
                     LKDAKIHQAILDSDVWFNVPILKNHGGSGLTISMKNYMGIVWDRRAFHMKGLQQCIAD
                     VCTLDKKPALNIVDAYR"
     gene            complement(801..>2787)
                     /locus_tag="PKY68_08450"
     CDS             complement(801..>2787)
                     /locus_tag="PKY68_08450"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOH25098.1"
                     /translation="NIRILAASMVEKAKSGHPGGAMGGADFINVLYSEYLIYDPNNPA
                     WEGRDRFFLDPGHMSPMLYSVLAMSGKFSMDELAQFRQWGSPTPGHPEVDVQRGIENT
                     SGPLGQGHAYAVGAAIAAKFLQSKFGEQMNQTIYTFISDGGVQEEISQGAGRLAGHLG
                     LDNLVMFYDSNDIQLSTEVSAVTSEDTAAKYKAWGWKVLEINGNDVNDIRKALDEAKK
                     TSGQPTLIIGKTIMGKGCLTEEGKSFENQCSTHGMPLSQAGASYDKTIENLGGNPADP
                     FRIFPEVETLYADRRKELEAIMKDKKAKKAAWAAQQPALAKKMQDWFNKVVPAVNWEA
                     IEQKEGIATRAASATVLGALAIQVENMVVASADLSNSDKTDGFLKKTKAMTKGDFSGA
                     FLQAGVAELTMACVCIGMGLHGGVIPACGTFFVFSDYMKPAVRMAALMEVPVKFIWTH
                     DAFRVGEDGPTHEPVEQEAQIRLLEKLKNHKGNNSMLVLRPADANECTVAYKMALENT
                     QTPTAFILSRQNIKNLPGSSYKQALQAEKGAYVVECDGDFDVILLASGSEVSTLVEGA
                     ELLRKDGIKLRIVSVPSEGLFRNQSQAYQEEVLPAGSKIFGMTAGLPVTLQGLVGDKG
                     KIWGLESFGFSAPYKVLDEKLGFNAANVYKQVKSFLA"
BASE COUNT          699 a          698 c          616 g          774 t
ORIGIN      
        1 acgataggca tctactatat taagtgccgg ttttttatcc agggtacaaa cgtcggcaat
       61 gcattgttgc aagcccttca tatgaaaggc ccttcggtcc cagactatgc ccatataatt
      121 tttcatagag atggttaatc cggagcctcc atgattttta agtataggta cattgaacca
      181 gacatcactg tccagtatag cctgatgaat cttggcatct ttaagtacct tacccttagg
      241 taattccacc tgacgataat aagattcctg atggccgggc agaactttgg ctccggcttc
      301 tttggcggcg gcttcgattc cgctgttttg ataacatttt acccaatcgt cgcaggtatg
      361 gtcaaatacc gttacctctg aggctccggc ggcgaaacac tgccttatga tttcgcttat
      421 aagtagagga ttggtattac cggctctttc gggtactcta tcccaaccaa tatttggttt
      481 gatgcatacc ttttgtccgc ttttgacaaa ttgattcatg cctccgaatt cggcaatagc
      541 ccttctgaac atttgatcgg gttctccgcc cataacagca accaaatcaa cagcctgctc
      601 ggttttattt tcttcgggaa caagagctgc cagcgacttg gccggtttta ctgccagtcc
      661 tactcccgtt aaagcgaaaa tcttcaaaaa atttcttctg ttcatggcta aatagttgta
      721 gtttttaaga taaaatttcg ggaaaccctg gcaaatatac catagtttcc cgaaaaaaat
      781 aggctataaa caattattaa ttaagccaaa aaggatttta cctgtttata aacattggca
      841 gcgttgaagc ccagcttttc gtccagaact ttgtagggag ccgaaaaacc gaacgattcc
      901 aatccccaga tcttcccttt gtcgccaacg agtccctgaa gagtaaccgg caaaccggcc
      961 gtcataccaa agattttgga accggcaggt aaaacctctt cctgataggc ctggctttgg
     1021 ttacggaaca aaccttcgga aggaacagat actatacgca gtttaatacc gtctttacgc
     1081 aataattctg caccttcgac cagtgtagaa acttccgaac ccgaggccag caggataaca
     1141 tcaaaatcac cgtcacattc gaccacataa gcaccctttt cggcttgtag ggcttgtttg
     1201 tacgaactac cgggtaaatt tttaatattc tgacggctca aaataaaggc tgtaggagtt
     1261 tgcgtatttt ccagggccat tttgtatgca acggtacact cattggcatc tgccggacgc
     1321 aaaaccaaca tggaattgtt gcccttgtga tttttgagtt tttccagcag acggatttga
     1381 gcttcttgtt caacaggctc atgcgtgggc ccgtcctcac ctactctgaa tgcatcgtga
     1441 gtccagatga atttaacggg aacttccatc aaagccgcca tacgaacagc cggtttcatg
     1501 tagtcggaaa aaacaaagaa cgttccgcag gcgggaatca cacctccgtg cagtcccata
     1561 ccgatacaga cgcaagccat ggtaagctcg gcaacaccgg cttgcagaaa ggctccgctg
     1621 aaatcgcctt tggtcatggc cttggttttt ttgaggaaac catcggtctt gtcggaattc
     1681 gacaaatcgg ccgaagccac caccatgttt tccacttgaa tagctaatgc accaagcacg
     1741 gtagcggagg cagcacgggt agcgatgcct tctttttgct cgatggcttc ccagttaacg
     1801 gcgggaacga ccttattgaa ccaatcctgc attttcttag ccagtgcggg ttgctgggca
     1861 gcccaagctg cttttttggc ttttttgtcc ttcataattg cttccaactc tttgcgtctg
     1921 tcggcgtaga gcgtttctac ttcggggaaa atacggaaag gatcagcggg attacctccc
     1981 agattttcga tggtcttatc gtaactggct ccggcttgac tcagaggcat accatgggtg
     2041 gaacattgat tttcgaaaga tttgccttct tctgtaagac aacccttacc catgatggtc
     2101 ttaccaataa tcagagtggg ttgtccgctg gtttttttgg cttcatccag tgctttacga
     2161 atatcattga catcgtttcc gtttatttcc agcactttcc aaccccaggc tttatatttg
     2221 gcagccgtat cttcactggt aactgccgat acttccgtcg aaagttgaat atcgttggag
     2281 tcgtaaaaca tcaccagatt atccagaccc aaatgacccg ccaaacgtcc ggctccctga
     2341 gaaatctctt cctgtacacc tccgtcggaa ataaaggtgt agatggtttg attcatctgt
     2401 tccccaaatt tgctctgcag aaatttggcg gcaatggcgg cacctacggc ataggcatgc
     2461 ccctgaccca gggggcctga ggtattttct atgcctcttt gcacatccac ttcgggatgg
     2521 cccggtgtag ggctgcccca ttggcggaac tgagccagtt catccatgct gaactttccg
     2581 ctcatggcca gcaccgagta caacatggga gacatgtgtc cgggatccag aaagaagcgg
     2641 tcgcgtcctt cccaagcggg attattagga tcgtaaatga ggtattcgga ataaagtacg
     2701 ttgatgaaat cagctccgcc cattgcccct ccgggatggc cggatttcgc cttttcaacc
     2761 atagatgcag ccaaaatgcg aatattg
//
LOCUS       DAOSGL010000303         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_35243_length_2751_cov_4.172107, whole genome shotgun sequence.
ACCESSION   DAOSGL010000303 DAOSGL010000000
VERSION     DAOSGL010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35243_length_2751_cov_4.172107"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /locus_tag="PKY68_08455"
     CDS             <1..1842
                     /locus_tag="PKY68_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH25099.1"
                     /translation="RYMYANSITLLRNRQDLLPFRHLERKSLAVVSLERPAETPFVKM
                     LRNYSSLEAFSLMPGNELDKRDLKQTFGQYDKIIVCVYSGNTNQIEWLSRFNGLNNLC
                     VCYFISPYRMQVFDRALSEPDAVLCAYEESLPAQEAAAQAVFGGTALRGVLPAPIGTV
                     YPAGHGLQTEKTRLSYGIPEESGMSAPVMSKIDSIVEDALNQGAMPGCQVVVAKNGRV
                     VWNRAYGWIDSERSRPTNTDDVYDLASLTKALVCTPAIMSLYEQGKIKLDEPLSNHLP
                     VLRDTDKEHLTYRNVLYHQARLSPFVPFYQYLLDTTSYPGPFLSTKPDSLHPMRFDEF
                     CWIPLNFSFKPELVSGEYKGPYLLQAAENFYVNASFRDSVCKIIAETSLLNNTRYTYS
                     DLGFILMGFSAETLLGKTLDEWVENELFAPLGAETTMYQPLKKIAPGRIAPTTLDTCL
                     RKQLLRGYVHDEAAAFLGGVAGNAGLFSSASDLVKVLQLFLDEGTYGSVRFFEPSTLR
                     YFTSKRSNLSRRLLGFDGAETNKNKIQQVAPSASRKTYGHIGFTGTCFWVDPVHELIY
                     IFLSNRVHPDRNNNLLSKLDVRPKIQEVLYESMEAYNKKQTSLQNGL"
     gene            1925..>2751
                     /locus_tag="PKY68_08460"
     CDS             1925..>2751
                     /locus_tag="PKY68_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOH25100.1"
                     /translation="MIQKLTLQAELVRKRTLEIIYGAKAGHTGGSLSAIEVLTALYFK
                     VLKLDPLRPDWPERDRFVMSKGHSVEALYCVLSARGFFPDAVLDTFGQFNSPLFGHPT
                     RKVPGVELNSGALGHGLSVAVGMALAARMDKSSYRCYALMGDGEQGEGSVYEAAMAAG
                     HYRLDKLTAIIDRNHLQISGKTEDVMTLEPMDERWKAFGWEVFETEGNDIAALLDCFE
                     RSEKVKDKPKLIISHSIKGKGVSYMENIAKWHHGVPNSEQFELAMKEIDSRIEKITH"
BASE COUNT          696 a          629 c          691 g          735 t
ORIGIN      
        1 cgctatatgt atgcgaattc catcactttg ttgcgcaacc ggcaggactt gttaccgttc
       61 agacacctgg agcgaaagtc cttggccgtg gtctctttgg aaaggccggc ggaaactcct
      121 tttgtgaaaa tgctccggaa ctacagttct ctggaggctt tttcgctgat gcccggcaac
      181 gaactggaca aaagggacct gaaacaaacc tttggccaat acgacaaaat catcgtttgc
      241 gtttactcgg gcaataccaa tcagatagaa tggctcagcc gtttcaacgg attgaacaat
      301 ctttgcgttt gttattttat atcgccctac cgtatgcagg tgttcgaccg ggctttgagt
      361 gaacccgatg ccgtgctctg tgcttacgag gaaagtctgc ccgctcagga agcggccgcc
      421 caggctgtgt tcggcggaac agccctgcgg ggagtgttgc cggctcccat cggaaccgtt
      481 tatcctgcag gacatggatt gcaaacggaa aaaacccgtt tatcctacgg tatccccgaa
      541 gaaagcggta tgagtgctcc ggtgatgagt aaaatcgaca gcattgtaga agacgcactg
      601 aatcagggag cgatgcctgg ttgtcaggta gttgtggcca aaaacggcag agtagtgtgg
      661 aacagagcct acggttggat agattcggaa cgcagccggc caaccaatac ggacgacgtg
      721 tacgatctgg cttcgctgac caaagccctg gtttgcacac cggcgatcat gagtttgtat
      781 gaacagggga aaatcaagct ggatgagcct cttagtaatc atttacctgt tttgcgcgat
      841 accgataagg aacatcttac ttaccgcaac gttttgtatc accaggcccg cttatctcct
      901 tttgttcctt tttatcaata tttgctggat accaccagct atcccggacc ttttctctcg
      961 accaagcccg attcgctgca tccaatgcgt tttgatgagt tttgttggat tccgcttaat
     1021 ttcagtttta aaccggaatt ggtgtctgga gaatacaaag ggccttattt gctccaggct
     1081 gcagaaaatt tttatgtaaa tgcttcgttt agagatagtg tttgtaaaat cattgcggag
     1141 acttcgcttc tgaacaacac acgctatacc tacagtgact tgggttttat tctgatgggc
     1201 ttttccgccg aaactttgct gggaaaaacc ctggacgaat gggtggaaaa tgagttgttt
     1261 gcccccttag gggctgaaac caccatgtat caacccctca aaaagatagc ccccggacgt
     1321 atagctccta caaccttgga tacctgtctt aggaaacagt tgctgagagg atatgttcac
     1381 gacgaggctg ccgccttttt gggaggagtt gccggaaatg ccggtctgtt ttcttcggcc
     1441 tctgacttag tgaaggtttt acaacttttt ctggacgagg gcacttacgg ttccgtgcgt
     1501 ttttttgaac cctccactct gcgttatttt acttcgaaac gcagtaatct gagccgccgc
     1561 ttgctgggct ttgacggagc agaaaccaac aagaacaaga ttcagcaggt agctccttcg
     1621 gcttccagaa aaacctatgg acacatcggt tttacgggaa cctgcttttg ggtggatccc
     1681 gttcatgaac tgatttatat tttcctgagt aatcgcgtac accccgacag aaacaacaat
     1741 ttactgtcga aactggatgt caggcccaaa atacaagaag tgttgtacga atccatggaa
     1801 gcatacaaca aaaaacaaac aagcttgcag aacggcttgt gaagattttg cttttttgtc
     1861 tttttgcata cgataattct gtttaccttt gaagcttctt ttatggagca aataaccctt
     1921 aagtatgatt cagaagttga cgttgcaggc ggagcttgtt cgcaaaagga ccctcgaaat
     1981 tatttatgga gccaaagccg gacatacagg cggctcttta tcggccattg aggtattgac
     2041 cgccttatat ttcaaagtgt taaaactgga tcctcttcgt cccgactggc cggagcgcga
     2101 tcgttttgtg atgagtaaag ggcatagtgt agaagccttg tactgtgtgt tgtcggccag
     2161 aggatttttc cccgatgccg ttttggacac cttcggccaa tttaattctc cgctcttcgg
     2221 acatcccacc cgcaaggtgc ccggagttga actgaacagc ggtgccttgg gtcatggttt
     2281 gtcggtggct gtaggaatgg ctctggcagc ccgtatggac aaaagcagct accgctgtta
     2341 tgcgcttatg ggcgacggag aacagggcga aggctctgtg tacgaagccg ctatggccgc
     2401 gggacattac cgtttggaca agcttaccgc catcatcgac cggaatcatc tgcagatcag
     2461 tggtaaaact gaagacgtga tgactcttga accaatggat gagcgttgga aggctttcgg
     2521 ttgggaagtg ttcgaaacag aggggaacga tatagcagct ttgctggatt gttttgaacg
     2581 cagtgaaaag gtcaaagaca agcctaaact gattatctcc cacagcatta aagggaaagg
     2641 cgtttcttac atggaaaata tagccaaatg gcatcacggg gtgcccaatt ccgagcaatt
     2701 tgagttggct atgaaagaaa tagattcccg tattgaaaaa attacccatt a
//
LOCUS       DAOSGL010000304         2748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_35304_length_2748_cov_3.144820, whole genome shotgun sequence.
ACCESSION   DAOSGL010000304 DAOSGL010000000
VERSION     DAOSGL010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35304_length_2748_cov_3.144820"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1925)
                     /locus_tag="PKY68_08465"
     CDS             complement(<1..1925)
                     /locus_tag="PKY68_08465"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HOH25101.1"
                     /translation="MKKFLLFLCLSVVTVMFSYGQRLMENLDRGLTAVKVSNGVFVSW
                     RMLGSEWNNTQYNLYRNGVKLNTEPLSVSNYLDEGGSNASTYTLGLVKEGVESPEPGN
                     YAVWNKQYLEIPMRQIIKNGIDYGPLYELNDATAADLDGDGQYEIIVKRINSDFSVAN
                     DSAFSYFEAYKLDGTLLWAIDIGPNLLSSGHVETNIAAFDWDEDGKAEVIMRAADGTI
                     DGKGNVIGSPTANYRPYISQSANMQFMTQGPEFLCLFDGETGELLDKVDYIARGNVND
                     WGDNYGHRANKFFFGAPYLDGRRPSILTTRGIYTRIVMRAYDVVDKKLQKRWLSDFDT
                     NNSLWSAYAYQGNHNYTIADVDGDGRDEIVYGSMTVDDNGRGLYSTGYGHGDAMHVSD
                     FDPYHRGLEIFACLEASPHWGAAFRDGATTETFIKHVRGADCGRCMAANVTDDYPGAE
                     LWADGKMWSATTRELVGSSGGTQNFAIYWDGDLLRETFDYTNLNDVTGGYGYGTPAVF
                     KYNKGSVSNIFTATGTATNNYTKGNPCLQADLFGDWREEMVLRSTDNKYLRIYTSVVP
                     SPWPIYTLMHDHQYRQAICWQMCGYNQPPHTSFFLGEREGITAPPPPWISNGKTEVDG
                     FISGDHQGKELMLANTQG"
     gene            complement(1953..>2748)
                     /locus_tag="PKY68_08470"
     CDS             complement(1953..>2748)
                     /locus_tag="PKY68_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOH25102.1"
                     /translation="AGQTYIALYNICGDPALIKNIKISMDMLVNTPQNNDWSWIDAIQ
                     MAMPVFAQLGQLTGDKRYYEKAYQMYMFTRNRHGSEGLYNPKEGLWWRDADFCPPYAE
                     PNGKNCYWSRGNGWVYAALARYMEIAPADEKHRDQYLKDFKAMSKALLACQREDGYWN
                     ISLHDPGHFGGKETSGTALFVFGMTWGIRHGILPAKIYLPAVLKGWKALEEAVHPNGF
                     LGYVQGTGKEPKDGQPVGYDIEPDFDDYGLGCFLLAGSEIYKLDLK"
BASE COUNT          594 a          698 c          734 g          722 t
ORIGIN      
        1 ccctgggtgt tggccagcat gagctctttt ccctgatggt caccgctgat aaaaccgtcg
       61 acttccgttt taccgttgct tatccaaggg ggagggggag cggtaatacc ttccctttcg
      121 cccaaaaaga agctggtatg cgggggctgg ttgtagccgc acatttgcca gcaaatggcc
      181 tgacggtact ggtggtcgtg catcagggta taaatgggcc agggcgatgg aaccacactg
      241 gtatagatgc gtaggtattt gttgtcggtg ctgcgcaaga ccatttcttc gcgccagtcg
      301 ccaaacaggt ccgcctgcag gcagggattt cccttagtgt agttgttggt ggctgtgccg
      361 gtggcagtga atatattgct cacactgccc ttgttgtatt taaacactgc cggcgttccg
      421 taaccgtagc cgccggtaac atcgtttaga ttggtgtaat cgaaggtttc tcttagcagg
      481 tctccgtccc agtagatggc aaagttctgt gttccgccgg agctgcctac caattctctg
      541 gtagttgcac tccacatctt tccgtctgcc catagttccg ctccgggata atcgtccgta
      601 acattggctg ccatacaacg gccgcagtct gcaccgcgga cgtgtttgat aaaggtttct
      661 gtggtggcac cgtcgcggaa ggcagctccc cagtggggag aggcttccag gcaggcaaaa
      721 atctcgaggc cccggtggta aggatcaaaa tcgctgacgt gcatggcatc gccgtgaccg
      781 taaccggtag agtacaggcc cctgccgttg tcgtctaccg tcatcgatcc gtaaacgatt
      841 tcatcgcggc cgtcgccgtc aacgtcggct atggtgtagt tgtgattgcc ctggtaagcg
      901 taggccgacc ataaactgtt gttggtatca aagtccgaca gccagcgttt ttgtaatttc
      961 ttgtcgacca cgtcgtaagc gcgcatcact atgcgggtat agatgcctct ggtagtaagt
     1021 atgctgggac ggcgtccgtc cagataggga gcgccgaaaa agaatttatt ggcgcgatgg
     1081 ccgtaattgt cgccccagtc gttgacgttg ccccgggcaa tgtaatcgac tttatcgagc
     1141 agctcgccgg tttctccgtc gaaaaggcat aagaactccg gcccttgtgt cataaactgc
     1201 atattggcag attggctgat gtagggacgg taattggctg ttgggctgcc tataacattg
     1261 cctttgccgt ctatggttcc gtcggccgcc cgcatgataa cttcggcttt gccgtcttcg
     1321 tcccagtcga aggcagcgat attggtttca acgtgtccgc tgctcaacag gttggggccg
     1381 atatcaatag cccaaagcag ggttccgtcc agtttatagg cttcgaaata gctgaaggca
     1441 ctgtcgttgg ctaccgaaaa gtcgctgttg atgcgtttga caataatttc gtattgtccg
     1501 tcgccgtcga gatcggcagc cgtagcatcg ttcagttcgt agagtgggcc gtagtcgatg
     1561 ccgtttttga tgatctggcg catggggatt tccaggtatt gtttgttcca gaccgcgtaa
     1621 ttgcccggtt cagggctttc aacaccttct ttgaccaatc cgagggtata agtcgaagca
     1681 ttgctgcctc cctcgtccag gtagttcgaa acactcagcg gttctgtgtt cagtttgacc
     1741 ccattgcgat ataagttgta ttgggtgttg ttccattccg aacccaacat gcgccaggaa
     1801 acaaaaacac cattggaaac tttcactgcc gtcagtcccc ggtcgagatt ttccataagc
     1861 cgctggccgt acgaaaacat cacagtgacc acgctcaaac agaggaatag aagaaacttt
     1921 ttcatcttct aatataaagt tgcagattaa tcttatttca gatccagttt atagatttcg
     1981 gaaccggcca gcaggaagca gccaaggccg taatcgtcaa aatcgggctc tatgtcgtaa
     2041 cccaccggtt gtccgtcttt gggttcttta cccgttccct gcacatagcc taaaaaaccg
     2101 ttgggatgta cggcttcttc cagggccttc cagcctttaa gcacagccgg caggtagatt
     2161 ttggcaggca aaataccgtg gcgtatgccc caggtcatgc caaaaacgaa aagagctgtt
     2221 cccgaagtct ctttgcctcc gaaatgtccg ggatcgtgca ggctgatatt ccagtagccg
     2281 tcttcacgct ggcaggccag cagagctttg gacatagcct tgaaatcttt caggtattga
     2341 tccctgtgtt tttcgtcggc gggagcgatt tccatatacc tggccagggc agcgtacacc
     2401 cagccgttgc ccctggacca ataacaattt ttaccgttgg gctcggcata aggaggacag
     2461 aaatcggcat ctctccacca aagcccctct ttgggattgt ataaaccctc ggagccgtga
     2521 cggttgcggg taaacatata catttgatat gccttttcgt agtaacgttt atcgccggtg
     2581 agctgcccca attgcgcaaa gaccggcatg gccatttgta tggcatctat ccaggaccag
     2641 tcgttgtttt gcggggtgtt gaccagcatg tccatggaga ttttaatgtt tttgatcagg
     2701 gcaggatcac cgcaaatatt gtataaagca atgtaggtct ggccggcg
//
LOCUS       DAOSGL010000305         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_35525_length_2737_cov_2.479493, whole genome shotgun sequence.
ACCESSION   DAOSGL010000305 DAOSGL010000000
VERSION     DAOSGL010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35525_length_2737_cov_2.479493"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKY68_08475"
     CDS             complement(<1..506)
                     /locus_tag="PKY68_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOH25103.1"
                     /translation="MLDIVTHIAGKLELGKEAVRNSLNLLDEGASIPFISRYRKENTG
                     GLNETQLASIADLYEELKGLEKRKESILQTIEEQGKLSPELKQQIESCTRKNELEDLY
                     LPYRPKRRTRAQIAREKGLEPLAALLMRQGPQDIGQLASRFTGSGLEVQEALDGASDI
                     IAEWVNEN"
     gene            complement(510..1790)
                     /locus_tag="PKY68_08480"
     CDS             complement(510..1790)
                     /locus_tag="PKY68_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH25104.1"
                     /translation="MTPEKRISPWSWVPSLYFGEGLPYVAVMTISVILYKRFGMSNTD
                     IAFYTAWLYLPWTIKFLWSPFVDIIKSKRWWIVLMQLLIGAFFGGVAFSLPTPFWIQA
                     SMAFFWLTAFSSATHDIAADGFYMHALETKQQAFFIGIRNTFYRLAMITGQGLIIILA
                     GKLEQQTGNPFGMEQIPYAWMTVFLVMAVFFVLLGTYHAVVLPRPQSDSERKHLSNKQ
                     LRQELMQSIKSFFQKKHILTAIAFMLLYRFSEAQLVKIASPFLLDSAEAGGLGLNTAQ
                     VGFAYGTLGIASLVIGGILGGIVISRRGLAASLWPMVLMLCLPNLIYVYFAFVRPESI
                     IVINLGVALEQLGYGYGFTAYTMYLLHFSQGPFKTAHYAICTAFMALGMMIPGMFAGA
                     LQEWLGYPLFFVWIVLATLPCFWVVALVKKQLKE"
     gene            complement(1780..>2737)
                     /locus_tag="PKY68_08485"
     CDS             complement(1780..>2737)
                     /locus_tag="PKY68_08485"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOH25105.1"
                     /translation="LLAQLGTNTQTAQTGPAAPENQPESPAAPENQPESPAAPENRSD
                     SPAERIRWYEREAHTQFDVCADDHCQRYQGLLEVDTPQVKTALSSTQGMVLCSGEDIC
                     DARFSKCCGGLTESFESCWADTRLTYLVSKPDLIPAPDRSSYADPEGFILGNPPSFCN
                     TNDKGILEQVLKDYDFQTRDFFRWTVSYDARELSDLFKQRSGLDLGLLSDLEALERGG
                     SGRIVRLKVTGSKGSLIIGKELEIRRCLSKTHLYSSAFVVDKVLDEQGKVRQFILHGA
                     GWGHGVGLCQIGAAVMAEQGYKFEQILEHYYPNTQIKQLYDS"
BASE COUNT          674 a          687 c          707 g          669 t
ORIGIN      
        1 ctgttttcgt tcacccactc ggctatgatg tcggaagcgc cgtccagagc ttcctgcacc
       61 tcgaggcccg aaccggtaaa acgggaggcc agttgcccga tgtcttgcgg tccttgtctc
      121 atcaataggg cggccagggg ctccaggcct ttttccctgg ctatctgagc ccgagtacgg
      181 cgtttggggc gatagggcag gtaaaggtct tccagttcgt ttttccgtgt acaggactct
      241 atttgttgtt tcagctcagg actgagtttt ccttgctcct ctatagtttg caggatgctt
      301 tctttgcgtt tttccaggcc tttgagttcc tcgtacaggt cggcaatact agccaattgc
      361 gtttcgttca gtcccccggt gttttctttg cgataacggc tgataaaggg aatgctggct
      421 ccttcgtcca gcagattcag actgtttctg acagcttctt ttcccagttc gagcttcccg
      481 gctatatgtg taactatatc gagcataatc tattctttca actgtttttt aaccagagca
      541 accacccaaa aacagggcag ggttgccagc acgatccaaa caaaaaacag aggataaccc
      601 agccattcct gcagggcgcc ggcaaacata cccggaatca tcatgcccaa ggccataaag
      661 gcagtgcaaa tggcgtaatg agcggttttg aaaggcccct ggctgaaatg caacaaatac
      721 atggtatagg ctgtaaaacc ataaccgtag ccaagttgct ccagagcaac ccccagattg
      781 attacaatga ttgattcagg gcggacaaaa gcaaagtaaa catagatcag attgggcagg
      841 cagagcataa gtaccatggg ccataaactg gcagccagtc ctctccggga aatgactatg
      901 ccgcccagta tgccgccaat caccaggctg gcaatgccca gggtgccgta ggcaaaaccc
      961 acctgggcag tgttcaagcc caagcctccg gcttcggcgc tgtcgagcaa aaagggcgag
     1021 gcaatcttga ccaattgagc ctccgaaaaa cggtagagca gcataaaagc gatggccgtc
     1081 agaatgtgtt ttttctgaaa gaaactttta atactctgca tcaattcctg acgcaattgc
     1141 ttgttactta agtgtttgcg ttcactgtcg ctctgcggac gaggcaggac aacagcatga
     1201 taggtgccaa gcaagacgaa aaagacggcc atgaccaaaa aaaccgtcat ccaggcgtag
     1261 ggtatttgct ccatgccaaa ggggttaccg gtctgttgtt cgagtttgcc cgccagaata
     1321 ataatcagcc cctgaccggt aatcatggcc aggcgataaa aagtattgcg aatgcctata
     1381 aagaaagctt gttgtttggt ttccagggca tgcatataaa agccgtcggc tgcaatatcg
     1441 tgagtggccg aactgaaagc cgtcagccag aaaaaagcca tgctggcctg aatccaaaag
     1501 ggggtgggca gactgaaagc cacacctccg aaaaaggctc cgatcagcag ttgcatcagt
     1561 actatccacc aacgtttgct tttgatgata tctacaaaag gactccaaag aaatttaatg
     1621 gtccagggca ggtagagcca ggcagtgtaa aaagcgatgt cggtattgga catgccaaag
     1681 cgcttgtaga gtattaccga aatggtcatc acagctacgt aaggcaggcc ttcgccaaaa
     1741 tacagactgg gaacccagct ccagggagag attctctttt caggagtcat acaattgctt
     1801 tatttgcgtg ttagggtagt aatgttcaag tatttgttca aatttgtaac cctgttcggc
     1861 cataacggca gctcctatct gacagagtcc tacgccgtgt ccccagccgg ctccatgcag
     1921 tatgaattgc cggactttgc cttgttcgtc cagtacttta tccaccacaa aagccgagct
     1981 gtagaggtgg gttttggata aacagcgtcg tatttcgagt tctttgccga tgatcaggct
     2041 acctttactg cccgttacct taaggcggac tattcgtccg gaaccgcctc tctcgagggc
     2101 ttccaggtcg cttagtaaac caaggtccaa tccggaacgc tgtttaaata agtcggaaag
     2161 ctcccgggca tcgtagctta ccgtccagcg aaaaaagtcc cgggtttgaa aatcatagtc
     2221 tttgagcacc tgttccaata tgcccttgtc gttggtgttg cagaaagagg ggggattgcc
     2281 cagtataaaa ccctccggat cggcatagct gctccggtcg ggggccggta tcaggtcggg
     2341 ctttgatacc agataggtta gtctggtgtc ggcccagcaa ctctcgaagc tttcggtcag
     2401 acctccgcaa cattttgaga aacgggcgtc gcaaatatcc tcccccgaac agagaaccat
     2461 accttgggta gagctgagcg cggtttttac ctgtggagta tcgacttcca aaaggccttg
     2521 ataacgttgg caatgatcgt cggcgcaaac atcgaattga gtatgtgctt cgcgctcgta
     2581 ccagcgaata cgttcagccg ggctgtcaga ccggttttcc ggggcagccg ggctttctgg
     2641 ttggttttcc ggggcagccg ggctttctgg ttggttttcc ggggcagccg ggccggtttg
     2701 ggccgtttga gtattagtgc ccagttgagc cagcaac
//
LOCUS       DAOSGL010000306         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_36094_length_2707_cov_3.902715, whole genome shotgun sequence.
ACCESSION   DAOSGL010000306 DAOSGL010000000
VERSION     DAOSGL010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36094_length_2707_cov_3.902715"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKY68_08490"
     CDS             <1..346
                     /locus_tag="PKY68_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HOH25106.1"
                     /translation="NPVAIRYQSSLLDNLYKLKSLFSAEEYEELAQAKLQKVRIIEQY
                     TDEIQQLVESMVDARKVANKIEDDREKAIAYHDTVEPFLDEIRQYIDKLELMVDNELW
                     PLPKYRELLFIK"
     gene            358..1332
                     /locus_tag="PKY68_08495"
     CDS             358..1332
                     /locus_tag="PKY68_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOH25107.1"
                     /translation="MKRIFLLCCLSFSLLAFAAQEESKPAYDLVVAQDGSGDFTSIQE
                     AIHSLRAVMTERKIVFIKKGIYREKIVVPTYCTNITLLGEDRDNTIIRWGDHANMPIP
                     GTETNMGTFRTYTLLVQGHGFRAQNLTIENDAPQLGQAVALHVEGDKASFINCRILGN
                     QDTIFAGSETSRQYYRDCYIEGTTDFIFGSATAYFLNCRIHCKRNSYITAPSTPEYRK
                     YGFVFKECKVTADEGIRVYLGRPWRPYGNTVFVQCELGACIRPEGWDNWRNPENEKTA
                     RFYECLNTGPGAWQEGRVSWAKNLKKNSLKEYTLKAVLAGKENWDPEK"
     gene            1545..>2707
                     /locus_tag="PKY68_08500"
     CDS             1545..>2707
                     /locus_tag="PKY68_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOH25108.1"
                     /translation="MKKSVFIPLITVLVCCLFVYLALPARNIEKQAWKQAERVLKNIK
                     DPTFPDRVLNILDFGAVVDIEDYSSTAINQAILKCHEQGGGKVLVPAGVFYTGPIELK
                     SNVCLHLEEGAVLKFSTNPEDYLPVVLSRWEGWDCYNLKPLIYAYKQENIAITGKGIL
                     DGQATIDNWWPWKGRREHGWEEGMISQEWNGDKEAGRNRLARMEAEEIPVEERIMSIE
                     DRLRPPFFQPYQCRNVLVEGITINRSPFWLLHPLLCENVTIRGVTMESHGPNNDGCDP
                     ESCKNVLIEDCYFNTGDDCIALKSGRNKDGFRWNTPAENIIIRRCVMKDGHGGVVIGS
                     EISGGCRNVWAEDCEMDSPNLDRVVRLKTNPLRGGTIENLFVRNIRVGECKESV"
BASE COUNT          745 a          624 c          682 g          656 t
ORIGIN      
        1 caatccggta gccatccgtt accagtcttc gcttctcgac aacttgtata aacttaaaag
       61 cctgttcagt gccgaagaat acgaggagct ggctcaggct aaactacaaa aggtaagaat
      121 catagagcag tataccgacg aaatacaaca attggtggaa tcgatggtcg atgcccgcaa
      181 agtggccaac aaaatcgaag acgaccgcga aaaggccatc gcttatcacg atacggtcga
      241 gccgtttttg gacgaaatcc gccagtacat cgacaaactg gaattaatgg tggataatga
      301 actgtggccc ctgcccaaat accgtgaatt attgtttatc aaataaaata tttagccatg
      361 aaacgaattt ttcttttatg ctgtctgagt ttttccctgc ttgcctttgc cgctcaggaa
      421 gagagcaaac cggcttatga cctggtggtg gcacaagacg gcagcggcga ttttaccagt
      481 attcaggaag ccattcattc cctgcgtgcc gttatgaccg aacgtaaaat cgtttttatc
      541 aaaaagggga tctaccgcga aaaaattgtg gtgcccactt attgtaccaa cattactctg
      601 ttgggcgaag acagagacaa caccattatt cgctggggcg atcatgccaa tatgcccata
      661 ccgggtaccg aaaccaacat gggtactttc agaacctata ctctgttggt gcaagggcat
      721 ggtttcaggg ctcaaaacct gaccatcgaa aacgacgccc ctcaattggg tcaggctgta
      781 gccttgcatg tggaaggcga taaggccagt ttcatcaatt gccggatttt gggtaaccag
      841 gataccatct ttgccggcag cgaaaccagc cggcagtatt accgcgattg ttacatcgaa
      901 ggcacgaccg attttatttt cggttcagcc accgcctatt ttttgaattg ccggatacat
      961 tgcaagcgca attcctatat tacggctcct tccacgccgg aataccggaa atacggtttt
     1021 gttttcaaag aatgcaaggt aactgccgac gagggcatac gggtttattt gggaaggccc
     1081 tggaggcctt acggcaatac ggtttttgtg cagtgtgaac tgggtgcttg catacgcccc
     1141 gaaggctggg acaattggag aaatcccgaa aacgaaaaga cggctcgttt ttatgaatgc
     1201 ctcaacaccg gccccggagc ctggcaggaa ggccgtgtaa gctgggctaa aaatctgaaa
     1261 aagaactctt taaaagaata cactctgaaa gctgttctgg ccggaaaaga gaattgggat
     1321 cccgagaagt aattttatac cgggctaaac gcaattcatt gctgaattat gcctgcaggt
     1381 acgcagcggg cgctattgtg ggcagtgcat ttattcgttg ccaacaggca atttccgata
     1441 cagaagaggc cgtaagtcga tcgctcgaaa tcttggatta taaacaaaaa tagatatatt
     1501 tgcgggcgat taaaccctat ccgtatcctt aatatatccc aattatgaaa aaaagtgtct
     1561 ttattcccct gattaccgta ctggtctgtt gtttgtttgt ttatcttgct cttcccgcca
     1621 ggaatattga aaaacaggcc tggaaacaag cggaaagggt gttgaaaaac atcaaagatc
     1681 cgacatttcc cgatcgtgtg ttgaatatat tggatttcgg agccgtagtc gacatcgaag
     1741 actacagcag cacagccatc aatcaggcca ttttgaaatg ccatgagcag ggagggggca
     1801 aagtgcttgt tcctgccggc gttttttaca ccggccccat tgagcttaaa agcaatgttt
     1861 gtctgcatct cgaagaggga gccgtactga aattttccac caatcccgag gactatttgc
     1921 cggtggtgtt gagccgttgg gaaggttggg attgctacaa tttgaagcct ctcatttacg
     1981 cctataagca ggaaaatatt gccatcaccg gcaaaggcat cctcgacggc caggctacga
     2041 tcgataattg gtggccctgg aaggggagaa gggaacacgg ctgggaagag ggtatgattt
     2101 cgcaggaatg gaatggcgac aaggaagccg gacgaaatcg cctggcccgc atggaagccg
     2161 aagaaattcc cgtcgaagaa cgcataatga gcatcgaaga ccgcctgcgt cctccttttt
     2221 ttcaacccta tcaatgccgg aatgtgttgg ttgagggaat taccatcaac agatctccct
     2281 tttggttgct gcatcctttg ctttgcgaaa acgtaacaat ccgcggagtt accatggaaa
     2341 gccacggccc caacaacgac ggctgtgatc ccgaatcttg caaaaatgtg ctgatcgagg
     2401 attgttattt caacaccggc gacgattgca tcgctctcaa atcgggccgt aataaggacg
     2461 gttttcgttg gaatacgccc gccgaaaaca ttatcatacg ccgttgcgtc atgaaagacg
     2521 gacacggcgg ggtggtgata ggcagtgaaa tatcgggcgg ttgccgcaat gtctgggccg
     2581 aagactgcga aatggacagt cctaatctgg atcgcgtggt acggctcaaa accaatccct
     2641 tgcgcggagg caccatcgaa aatttgtttg tccgcaacat ccgggtagga gaatgcaaag
     2701 agtcggt
//
LOCUS       DAOSGL010000307         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_36203_length_2701_cov_4.275132, whole genome shotgun sequence.
ACCESSION   DAOSGL010000307 DAOSGL010000000
VERSION     DAOSGL010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36203_length_2701_cov_4.275132"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2701)
                     /gene="nifJ"
                     /locus_tag="PKY68_08505"
     CDS             complement(<1..>2701)
                     /gene="nifJ"
                     /locus_tag="PKY68_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOH25109.1"
                     /translation="AERIIVAMGSITEAAKEVVDYLMQKGEKVGLISVHLYRPFSVKY
                     MKAVLPKTVKRIAVLDRTKEPGANGEPLYLDVVEAFAKNDALGLDKMPFIVGGRYGLS
                     SKDTTPAQILAVFENLSLPEPRNQFTIGIVDDVTFTSLPQEEEIALSGESIYEAKFYG
                     LGADGTVGANKNSIKIIGDNTDKYCQAYFSYDSKKSGGFTCSHLRFGDTPIRSTYLVN
                     TPNFVACHVQAYLNLYDVTKGLRQNGTFLLNTIWEGEELAKHLPAKVKRYFAKRNISV
                     YYINATQIAQEIGLGNRTNTILQSAFFRITKVIDVDLAIDQMKKFIVKSYGNKGEEIV
                     RKNYEAVDRGGEYKQLKVDSAWANLPDDPAVINNDPEFINKVVRPINAQDGDLLPVSA
                     FKGIEDGTWMPGTARYEKRGVSAFVPEWIAENCIQCNKCAYVCPHASIRPFVLDEAEA
                     AAAPGFETIEMKAPATMKGMQFRIQVSVLDCLGCGNCADVCPGNPRAGGKALKMVDLE
                     SQMSEASNWEYCVDKVKSKQHLVDTKQNVKNSQFATPLFEFSGACSGCGETPYLKLLT
                     QLFGDRQIVANATGCTSIYSGSVPSTPYTTNELGHGPAWANSLFEDFCEYGFGMQLAY
                     EKMRERLVKLMSDAQSCSRCSEEQKALFAEWLTDREDGDKSRVLADKIIDAVKGCDCD
                     YCKQISSLSKYLVKRSQWIIGGDGAAYDIGYGGLDHVIASGKNVNILVLDTEVYSNTG
                     GQSSKATPLGAIAKFAAAGKRIRKKDLGMIATTYGYVYVAQVAMGADQAQTLRAFREA
                     EAYDGPSLIIAYSPCINHGLKRGMGKSQAEAEAAVACGYWHLWRYNPQLEAEGKNPFI
                     LDSKEPDWSKFADFLKGEVRYSSVIKQYPDEAEELFKAAEESA"
BASE COUNT          640 a          675 c          658 g          728 t
ORIGIN      
        1 ttggcgcttt cttcggcagc tttaaacaac tcttcggcct catccggata ttgctttatc
       61 accgatgaat aacggacttc gcctttgagg aagtcggcaa atttactcca gtcgggttct
      121 ttggaatcga gtataaaggg atttttaccc tcggcctcga gttgtggatt gtagcgccac
      181 agatgccagt aaccgcaagc tactgctgct tcggcttcgg cctgcgattt acccataccc
      241 ctcttcagtc cgtgattgat acaaggcgag taagcaatta tcaacgaagg cccgtcgtag
      301 gcttccgctt cgcggaaagc tctgagggtt tgggcctgat cggctcccat ggctacctga
      361 gcaacgtata catagccata ggtggtggca atcattccca aatctttctt gcgtatacgc
      421 ttgccggcgg cggcaaattt agcaatagct cccaacggag tagctttgga cgactggcct
      481 ccggtgttgg aatacacttc ggtgtccagc accaggatat taacgttctt gcccgaggcg
      541 atcacgtggt ccaatcctcc gtaaccgatg tcgtaagcag caccgtctcc tccgatgatc
      601 cattgactgc gttttaccag atacttgctt aggctgctga tttgcttaca ataatcacag
      661 tcgcatcctt ttaccgcatc tatgatcttg tcggccagta cacggctctt gtcgccgtct
      721 tcacgatcgg tgagccattc ggcaaacaag gctttttgtt cttcggaaca acggctgcag
      781 gattgagcat cgctcataag cttaaccagg cgttcacgca tcttttcgta agccagttgc
      841 ataccgaaac cgtattcgca gaaatcttcg aacagcgagt tggcccaggc aggaccgtga
      901 cccaattcgt tggtggtgta aggcgtggaa ggtaccgagc ccgaataaat ggaagtacag
      961 ccggtggcat tggccactat ctggcggtcg ccaaacaatt gggtcaacaa tttgaggtaa
     1021 ggcgtttcgc cgcaacccga acaggctccc gaaaactcaa acaagggagt ggcaaattgt
     1081 gaattcttga cgttttgttt cgtgtcgacc aggtgttgtt tgcttttgac tttgtctaca
     1141 caatattccc aattggaagc ttcactcatc tggctttcga ggtctaccat cttaagggct
     1201 ttgcctccgg ctctggggtt gcccggacaa acatccgcgc aattgccgca acccaaacaa
     1261 tccaataccg aaacctgaat acggaattgc atacccttca tggtggcagg agccttcatt
     1321 tcgatggttt cgaacccggg agctgcggca gcctctgcct catccagtac aaaaggacga
     1381 atggaggcat gcggacagac gtaagcacat ttattgcact gaatgcaatt ttcggctatc
     1441 cattcaggta caaaggcgct gacacctctc ttttcgtacc gggctgttcc cggcatccag
     1501 gtgccgtctt cgatgccttt gaaggccgaa accggaagca agtcgccgtc ctgtgcattg
     1561 atgggacgaa ctactttgtt gataaattcg ggatcgttgt tgattaccgc ggggtcgtca
     1621 ggcagattag cccaggcaga atcaactttg agttgtttgt attcaccacc tctgtcgacg
     1681 gcttcgtaat ttttcctgac aatctcttcg cctttattac cgtaggactt gacaatgaat
     1741 ttcttcattt gatcaatagc cagatccaca tctataacct tggttattct gaagaaagcc
     1801 gactgcaata tggtgttggt acgattgccg aggcctatct cctgtgctat ctgggtggcg
     1861 ttgatataat aaacactgat gttccttttg gcaaaataac gtttgacctt ggcgggtaaa
     1921 tgcttggcca actcttcgcc ttcccagatg gtgttcagca ggaaagtacc gttctgacgc
     1981 aaacctttgg tgacatcgta aaggttcaga taagcctgca catgacaagc tacaaaattg
     2041 ggcgtattca ccaaataggt cgaacgtatg ggggtatcgc caaaacgcag gtgagaacag
     2101 gtaaaaccgc cggacttttt ggagtcgtac gaaaaataag cctgacaata tttatcggta
     2161 ttgtctccga taatcttgat ggaattctta ttggctccta ccgtaccgtc agcgcccagg
     2221 ccatagaatt tggcttcgta aatgctttcg ccgctcagtg caatttcttc ctcctgaggc
     2281 aaagaagtga acgtaacgtc gtcaactata cctatggtaa actggttgcg gggttcgggt
     2341 aaggaaagat tttcgaatac ggccaaaatc tgggcaggcg tggtgtcttt cgaagacaaa
     2401 ccgtagcggc cgccaacaat gaaaggcatt ttgtccagac ccaatgcatc gttttttgca
     2461 aaggcttcga ctacgtcgag atacagcggt tcgccattgg ctccgggttc tttggttctg
     2521 tccagtacgg caattctttt gaccgtcttg ggcagtaccg ctttcatata tttaaccgag
     2581 aaaggacgat agagatgtac ggaaatcagg cccacctttt cgcctttttg catcaaatag
     2641 tcgacgactt ctttggcggc ttcggtaatg gagcccatcg caacgataat acgttcggcg
     2701 t
//
LOCUS       DAOSGL010000308         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_36475_length_2687_cov_3.681991, whole genome shotgun sequence.
ACCESSION   DAOSGL010000308 DAOSGL010000000
VERSION     DAOSGL010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36475_length_2687_cov_3.681991"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1525
                     /gene="ligA"
                     /locus_tag="PKY68_08510"
                     /pseudo
     CDS             1..1525
                     /gene="ligA"
                     /locus_tag="PKY68_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267567.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
     gene            1831..2349
                     /locus_tag="PKY68_08515"
     CDS             1831..2349
                     /locus_tag="PKY68_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25110.1"
                     /translation="MVDFFLKRKIKQLRRQHSRNKRFMNYADLRRICILFDVADYEHI
                     VPLVDELLADGKDVIAWTYAARGVIAKDYPAYYNVISDRDLTFCYFPKKYLMNRYLGY
                     PADLLIDLCYTPHILNEYLVAVSPALYRLGLKTADPLWYDLIVDISGQEKEEMEKNAA
                     SVLFYLKSLRVK"
     gene            2394..>2687
                     /locus_tag="PKY68_08520"
     CDS             2394..>2687
                     /locus_tag="PKY68_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOH25111.1"
                     /translation="MKISINNKLRGMGVALITPFNEDDSIDFDALGRLVDYQLHNKTD
                     YLVVLGTTAETPTLSDQEKIAIKDFVIQRVNGRLPIVLGVGGNCTRSLVHQLET"
BASE COUNT          707 a          640 c          718 g          622 t
ORIGIN      
        1 aggagaagac tggccgcaat tggtcgagat caggggcgaa gtgctgatgc cctgggaagt
       61 gttcgaagcg ctgaacgaag aacgagaaaa gcaggaagaa aacctttttg ccaatcccag
      121 aaacgctgct tcgggcacat tgaaattgca aaattcggca acggtggctt cgcgtaagct
      181 cgaagcctgg ttctacgccg taatgggcga acacctaccc tttgagggac attacgaaaa
      241 cctgcaaaaa gcccgaagct gggggttgcg tgtttcggaa catacacgta aatgcagtag
      301 cctcgacgag gtgttcgact ttatacgtta ttgggatgta gaacgcaaaa aactgccggt
      361 ggctaccgac ggcattgtta tcaaggtcaa ttctctccgt caacaggagc aattgggttc
      421 taccgccaaa tcgccccgtt gggccatcgc ttataaattt caggccgaac aagcactgac
      481 ccaattgaaa tcattgagtt ttcaggtggg tagaacagga gccgttacac cggtggctaa
      541 cctggagccc gtgcttttat cgggcacggt ggtaaaaaga gcttcgctgc acaacgccga
      601 ttttatcagg gaactggatt tgcatttggg cgaccgggtt tttgtggaga aaggcggaga
      661 gattattccc aagattgtag gagtagataa ggactccaga aaagccggag catcggagcc
      721 ggtgcaattc atcgagcgtt gtccggaatg cggcagccct ttgcagcgta tcgaaggcga
      781 agccgctcat tattgcccca acactacggg ttgtccgccc caaatcaaag gcaggatttt
      841 gcattttgtt caccgtaagg cgatgaatat cgatggtttg ggcgaagaaa cggttgattt
      901 attgtaccag aaaggcttgg tcaacaaagc ttccgattta tatcgtctgc gaaaggatca
      961 actgacgggg ctcgaaagga tgggcgagaa gtcggccgat aatttgataa atagcatagc
     1021 cgcctcggtt tcggtgccct tcgaaagact cttgtttgcc ctgggaatac gttttgtggg
     1081 agaaaccgtc gctaaaaaac tggcgcgtac cctgggctct atggatgcct tgtgccgggc
     1141 cacttacgaa gaactcctac aggtggagga ggtgggcgaa aaaatagctg agagtgtatt
     1201 ggcctttttt gccgatgcct ccaatcgggc catgttggaa gagttccggc agctgggttt
     1261 gcagtttgaa tcggacaaga ccgtacaaaa gaccgaaggt ttgttgaaaa atcagaccgt
     1321 agtgatcagc ggaactttcg aaaaacacag ccgggacgaa tacaaagagc tgatagagca
     1381 ggagggagga agaatcgctt cctcggtcag ctccaaaaca gactttattt tggccggcga
     1441 aaatatgggg ccttccaaac tcgaaaaggc caaatcgctg ggtctgaaac tgatgtccga
     1501 aaaggaattt ctcgccttac tggccgccgg ctcagacacc gacaccggct cggacactga
     1561 cgccggctca gacaccgagg ccgggtcgga tgcggttccc ggcccagacg ctgtttcagg
     1621 cagcggcacc gatttcagtt cagtcgccga tccgggccgg gacgtggttt ccggcccagc
     1681 aacccctgct gagtctgttc caactactgc ccctgccggg agccctgccc tgtcaaaccg
     1741 gaacaagcag agcaaaaaat ccaacaccgg ctccatctcg agccccggtc tttttgacga
     1801 actctaatac taaacacaga ataggagact atggtagatt tctttttaaa acgaaagatc
     1861 aaacaattaa gacgccaaca cagccgtaac aagcgtttta tgaattatgc cgacttgcgg
     1921 agaatctgta ttttgtttga tgtggccgat tacgaacata tcgttcctct ggttgacgaa
     1981 ctgctggccg acggcaaaga tgtaatagcc tggacctatg ctgcgagagg ggtgattgct
     2041 aaggattatc ccgcctatta caacgtgatc agcgacaggg acctgacttt ttgctatttc
     2101 cccaaaaaat acctgatgaa tcgttacctg ggttatccgg ccgatctcct gatcgatctt
     2161 tgctatactc cccacatcct taacgagtat ttggtggccg tatcgccggc tttgtaccgc
     2221 ctgggattaa aaaccgccga tcccctctgg tacgatctga tagtggacat cagcgggcaa
     2281 gaaaaagaag agatggaaaa aaacgccgcc agcgtgcttt tttatcttaa gagtttacga
     2341 gttaagtagg ttttttatac ctttgcgcct cggattgctg caacccagag tgtatgaaaa
     2401 tttccataaa caataaattg agggggatgg gtgttgccct gataacacct ttcaacgaag
     2461 acgacagtat cgattttgac gctctgggcc gcctggtgga ttatcagttg cacaacaaaa
     2521 ccgattacct ggtggttctc ggtactacgg ccgagacacc caccctcagc gaccaagaaa
     2581 agatcgctat taaagacttt gtgatacaac gggttaacgg ccggcttccc atagtgttgg
     2641 gagttggggg caattgtacg cgcagcctgg ttcatcaatt agagacc
//
LOCUS       DAOSGL010000309         2686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_36500_length_2686_cov_4.077537, whole genome shotgun sequence.
ACCESSION   DAOSGL010000309 DAOSGL010000000
VERSION     DAOSGL010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36500_length_2686_cov_4.077537"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKY68_08525"
     CDS             <1..729
                     /locus_tag="PKY68_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HOH25112.1"
                     /translation="GPHPAGNVGVQINRIDPVNKGETVWTVNALDVLFIGRFFNKGRV
                     DFSRLVALTGPEVYEPRYYRCVAGIQLSALLKGNVHKEIPLRYISGNALTGTRVEMDD
                     YLGAYDSQVTVLHEGTDVHEFAGWVMPRLNMFSMSKTYFTGLVQKICPRKQFDADTRI
                     LGGERALIMSGEYEKVFPMDILPEKLVRACITRDLEKMEELGIYEVAPEDFALCEYVC
                     TSKVEVQKQVRTALDLLKAENGEE"
     gene            755..1957
                     /locus_tag="PKY68_08530"
     CDS             755..1957
                     /locus_tag="PKY68_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOH25113.1"
                     /translation="MKALRKLLDKIKPAFEKGGKLSMFHSVFDGFETFLFVPNRTSQS
                     GTHIHDSIDSKRAMFVVVIALIPALLFGMFNVGYQHYLAIGQAAGFFQTFFWGALAVL
                     PYIVVSYVVGLGIEFTIAQFRGEEIHEGFLVTGMLIPLIIPINTPLWMVALATAFAVI
                     FVKEIFGGTGYNIFNVALMARAFLFFAYPSKMSGDSVWVSLKDYFGFGQEQATIDVYT
                     GATPLGQAAIAGGDNLVLTGITGEPLSWWDTFLGLIPGSIGETSVLAILLGGILLIYT
                     GIASWKIILSVFAGGAFMAILLNIFAVNPYMEMPFWEHWCLGGFAFGAFFMATDPVTA
                     SRTEKGKWIYGFLIGLLAILYRVLNPAYPEGMMLAILFMNIFAKFIDYLVVQNNIKKR
                     LKRARIAQ"
     gene            1969..>2686
                     /gene="nqrC"
                     /locus_tag="PKY68_08535"
     CDS             1969..>2686
                     /gene="nqrC"
                     /locus_tag="PKY68_08535"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOH25114.1"
                     /translation="MNKNSNTYIFIYASVMVILVAAALALTNGLLKDQHDKNSEIDKM
                     TQILRSIKVEADKNNAESLFEQNIKTAYILNSAGQVDKESSPEAAFAVEMSGEVTKPL
                     EKRQLPVYEALVEGDTLYILPVYGAGLWGPIWGYVSLKNDRNTIYAASFSHSGETPGL
                     GAEISTEPFLAPFSGKVLYKEGEFRSIAILKSGQKADNQDAVDAISGGTITSKGVEDM
                     LLHCLGAYKAFFEQVETENTE"
BASE COUNT          693 a          593 c          669 g          731 t
ORIGIN      
        1 ggccctcatc ctgcaggcaa cgtcggtgtg cagatcaatc gcatcgatcc ggtaaataaa
       61 ggagaaacgg tctggaccgt aaatgctttg gatgtactct ttattggccg gttctttaac
      121 aaaggaaggg tcgatttttc gcgtttggta gcactgaccg gccccgaagt atacgagccc
      181 cgttattacc gctgcgttgc aggaattcag ctctcggctt tgctcaaagg caatgtacat
      241 aaagaaattc ctctgaggta tatcagcggc aatgccctca caggcacgag ggtagaaatg
      301 gacgattatt tgggcgccta cgatagtcag gtcaccgtgc ttcacgaagg tacggatgta
      361 cacgaatttg ccggctgggt aatgccccgt ttgaatatgt tcagcatgag caagacctat
      421 ttcaccggat tggtgcaaaa gatttgcccc agaaagcaat tcgatgccga caccagaata
      481 ctgggcgggg aaagagctct tattatgtcc ggcgaatacg aaaaagtgtt tcccatggat
      541 attttacccg agaaactggt gcgtgcctgt attacccggg atttggaaaa aatggaggaa
      601 ttgggcatat acgaagttgc ccccgaagat tttgctttgt gcgaatatgt ttgtacctct
      661 aaggtagaag tacaaaaaca agtccggact gccctggatt tgttaaaagc cgaaaacgga
      721 gaagaatagt agtcactaca aaaaacacca tactttgaaa gctttgcgaa agttacttga
      781 caagatcaag cccgcctttg agaaaggtgg aaaattatcg atgtttcact cggtttttga
      841 cggttttgaa acttttttgt ttgttcccaa tcgtacttcc caatcgggaa ctcatattca
      901 cgacagcatc gattccaaac gggccatgtt tgtcgtggtt attgctctga tcccggcttt
      961 gcttttcggg atgttcaatg ttggctacca gcactacctg gccattggcc aagctgccgg
     1021 tttcttccag acttttttct ggggagccct ggcggtgttg ccttatattg ttgtttcgta
     1081 tgtggtagga ttgggtattg agtttaccat tgcccagttc aggggcgaag aaattcacga
     1141 gggatttttg gtaaccggta tgttgatacc ccttatcatt ccaatcaaca cgcctttatg
     1201 gatggtggct ctggcgacgg cttttgccgt aatattcgtc aaggaaatat ttggaggtac
     1261 cggctataat atcttcaatg tcgccctgat ggcaagagcc tttttgtttt ttgcctatcc
     1321 ttccaagatg tcgggcgact ccgtatgggt cagtctgaaa gattatttcg gttttggaca
     1381 ggaacaagca acgatagatg tttatacggg agcaacacct ctgggacagg cagccatcgc
     1441 cggcggcgat aatctggtcc tgacaggcat aacaggcgaa ccgctgagct ggtgggatac
     1501 ctttctgggc ctgattccgg gctccatagg agaaacttct gttttggcca tacttttagg
     1561 cggaattcta ttgatataca cgggcattgc cagttggaag attattcttt cggtttttgc
     1621 cggcggcgct tttatggcta tcctgctcaa tatttttgcg gtaaatccct acatggaaat
     1681 gcccttctgg gaacattggt gcctgggcgg ctttgctttt ggagcctttt ttatggccac
     1741 cgaccctgtg actgcatccc gtaccgaaaa gggtaaatgg atttacggtt tccttatcgg
     1801 cctgttagcc atactctatc gtgtgctgaa tccggcttac cccgaaggta tgatgctggc
     1861 tattctgttt atgaatattt ttgccaaatt catcgactat ctggtcgtgc agaacaacat
     1921 caaaaaacgg ctcaaaaggg cgcgcatcgc tcaataatat ttaggactat gaataaaaac
     1981 agcaatacct atatttttat ttatgcttcg gtaatggtta ttctggtcgc cgccgctctg
     2041 gctctgacca acggactgct caaagaccag cacgataaaa acagtgagat cgacaaaatg
     2101 acgcagatat tacgctccat aaaagtggaa gccgataaaa acaatgccga aagtcttttt
     2161 gaacagaaca ttaaaacagc ctacatactg aacagtgccg gccaggtgga taaagaatca
     2221 tcgcccgaag ctgcttttgc cgttgaaatg tccggcgaag taaccaagcc tttggaaaaa
     2281 cgccagctcc cggtctatga agctctggta gaaggagaca cactctatat tctgcccgta
     2341 tacggagcag gcctttgggg gccgatttgg ggttatgtct ccttgaaaaa cgacagaaac
     2401 accatttatg ctgcgtcgtt ctctcactcg ggagaaacgc ccggattggg ggcagaaata
     2461 agcaccgagc cattcctggc gcctttttcg ggcaaggtgt tgtataaaga gggtgaattt
     2521 cgttccatag ctatactcaa gtccggtcaa aaagcggaca atcaggatgc ggtagatgcc
     2581 atttcgggag gaaccattac cagcaaagga gtcgaagata tgttgctcca ttgcctggga
     2641 gcctataagg cgttttttga acaagttgaa acagagaaca cagaga
//
LOCUS       DAOSGL010000310         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_36558_length_2684_cov_4.007607, whole genome shotgun sequence.
ACCESSION   DAOSGL010000310 DAOSGL010000000
VERSION     DAOSGL010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36558_length_2684_cov_4.007607"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..1781
                     /locus_tag="PKY68_08540"
     CDS             423..1781
                     /locus_tag="PKY68_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HOH25115.1"
                     /translation="MKFFRTVLLLTTLSLFFCNCRKEQDFRLWAPTPPMGWNSWDCYG
                     PTVEEHEVKANADYMAEKLKEYGWEYVVVDIRWFVENDKAGGYNQTDPRYVMDEYGRY
                     LPALNRFPSASDGKGFKPLADYVHGKGLKFGIHIMRGIPVEAVNKQLPIKGAKGITAD
                     QIFSPDLQCAWLRDNYTIQADKPGAQEYYNSIFDLYASWGVDFIKIDDLSRPYHQAEI
                     ELIRKAIDQCGRKIVLSTSPGATPLSAAEHVSKHANMWRMVDDVWDSWWHIAHLMEVA
                     QGWYPYIKEGTWPDCDMIPLGRISIRGERGGDRMSRLSPDEQYLLMTFFCIFRSPLMF
                     GGDLPSNDDFTLSLLTNQEVLRMHRESSNVRQLYRKDNKIAVSSENTVTGEKYLALFN
                     MDDRNTQNLAVNIAELGLQGNCRATELWQNQTLTFSDSIHCDLRPHAAVLYKIHPAEN
                     PD"
     gene            1860..>2684
                     /locus_tag="PKY68_08545"
     CDS             1860..>2684
                     /locus_tag="PKY68_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH25116.1"
                     /translation="MYEKDKRIVMTLDAGGTNFVFSAVQAYREIVEPVRLPSSADNLE
                     QSLDNIEQGFLQVRLQLKQAPAAISFAFPGPADYSRGIIMDLNNFPAYRGGVALGAFL
                     KNRFNLPVFINNDADLFTYGECISGALPFVNRRLEAAGSTKRYRNLIGFTFGTGFGCG
                     ITSNNELYLGDNGSAGEVLLLRHKTKRDIIIEDGVSIRAIKRVYRELTGDTRELEPLD
                     VFNIADGKQEGDVQAAQKTFEIFGVMAAEGIAAVTTVLDGLIVLGGGLCGARKFFMP"
BASE COUNT          702 a          670 c          657 g          655 t
ORIGIN      
        1 gcaaccgcaa gataacgatt atcacaggaa cgaaacagca atgtacagca aatgtagttg
       61 atccgagccg gggctgtttg aatttttgtc ccaagcgcta cgaaaatcgt ccagaattta
      121 gtttggtcat tcgttaagca tcgcaaaaac accttttgtg tccggtagaa tctttccgtt
      181 acgggaacaa atttttatat ttgctcctgc tttttggatt tgtttcgcag tcgtttgggc
      241 ttcaggtcgc ttggcttcac agttccttgg ggttcctgtc gcttagaccc gaactccttt
      301 aggctatagt ccctggagct cgtggtcgtt tgagcttcag gttgtttgga ctcccggaat
      361 accgaaacac caaaattcaa acgaaacacc aaaatgtaaa cgaacactaa aatttagata
      421 ctatgaaatt cttccgaaca gttttgttgt tgacaacact aagtcttttt ttctgtaatt
      481 gccgcaagga acaggatttt cgcctctggg cccctactcc gcccatgggc tggaacagtt
      541 gggattgcta cggccccacg gtcgaagaac atgaagtaaa ggccaatgcc gattacatgg
      601 ccgaaaaact caaagaatac ggttgggaat acgttgtggt ggatatccgc tggttcgtcg
      661 aaaacgataa agccgggggt tataatcaga ccgacccccg ctacgttatg gacgaatacg
      721 ggcggtattt gccggctctg aaccgttttc cctcggcatc cgacggcaaa ggattcaaac
      781 ccctggccga ttacgtacac ggcaaaggcc ttaaattcgg catccatatc atgaggggca
      841 ttccggtaga agcagtaaac aaacaactgc ccatcaaggg tgccaaaggt attactgccg
      901 accaaatttt ctctcctgac ttacaatgcg cctggctaag agacaattat accattcaag
      961 ccgataagcc cggagcacaa gagtattata attccatttt tgacctttat gcttcctggg
     1021 gcgtggattt tatcaaaata gacgatctct cgaggcctta tcaccaggcc gagattgaac
     1081 tgattcgcaa agccatagat caatgcggac ggaaaatagt gttgagcact tcgcccggag
     1141 ccactcccct gtcggccgcg gagcacgtga gcaagcacgc caatatgtgg cgtatggtcg
     1201 acgatgtctg ggattcctgg tggcatatcg cccacctgat ggaagtggcc cagggctggt
     1261 atccctacat caaagaaggc acctggcccg actgcgacat gattcctctg ggacgcatat
     1321 ccatacgggg cgaacggggc ggagatcgca tgagccgctt aagtccggac gaacaatacc
     1381 tgctgatgac cttcttctgt atattccgtt ctcctttgat gtttggaggc gatctgccaa
     1441 gcaacgacga ttttaccctc tcactgctta ccaaccaaga agtgctcagg atgcaccgcg
     1501 aaagctccaa tgtgcgtcaa ctctaccgga aagacaataa aattgccgta agctccgaaa
     1561 acacggttac gggcgaaaag tacctggctt tgttcaatat ggacgaccgg aatactcaga
     1621 atctggcggt gaatatagcc gaacttggac tgcaagggaa ttgtcgcgct accgaactct
     1681 ggcagaatca gaccctgact ttcagcgaca gcatccactg cgatctgaga cctcatgctg
     1741 ccgtgctcta caaaatccat ccggcggaaa atcccgacta agtgtccact cgattataat
     1801 cgggcttaaa cagccacttg ctgcacactc ttgaacctgc attaactacc atatgcctta
     1861 tgtacgaaaa agataaacgc atagttatga ctctcgacgc cggaggaacg aattttgttt
     1921 tttcggccgt acaagcttat cgcgaaatag tagaacctgt tcgtctgccc tcgtcggcag
     1981 acaatctgga acagagtctc gataatatag aacagggttt tttgcaggtt cggttgcaat
     2041 tgaaacaagc tccggcagcc ataagttttg cttttcccgg accggccgac tacagccggg
     2101 gcatcatcat ggatttaaat aattttccgg cctacagggg aggtgtggct ttgggcgctt
     2161 tcttaaagaa caggttcaac ttgcctgtat tcataaacaa cgacgccgac ctgtttacct
     2221 acggtgaatg catatccggc gcactgcctt ttgtcaatcg acgcctcgaa gcggccggca
     2281 gcaccaaacg ctatcgcaac ctgataggat tcacctttgg tacgggcttt ggttgcggca
     2341 tcaccagcaa caacgaattg tacctgggag acaacggatc ggcgggcgaa gtactgctgc
     2401 tgcgccacaa aaccaaacgc gacatcatca tcgaagacgg agtgtccata cgggccatta
     2461 aaagagttta ccgggaattg acgggcgata cgcgcgaact ggaacctttg gatgttttca
     2521 acatcgccga tggaaaacag gaaggagatg ttcaagcggc ccaaaaaacc tttgagattt
     2581 tcggcgttat ggccgccgaa ggcattgcgg ctgttactac agtgctcgac ggactgattg
     2641 tattgggtgg aggcctgtgt ggagcccgga aattttttat gccc
//
LOCUS       DAOSGL010000311         2673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_36771_length_2673_cov_3.134072, whole genome shotgun sequence.
ACCESSION   DAOSGL010000311 DAOSGL010000000
VERSION     DAOSGL010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36771_length_2673_cov_3.134072"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PKY68_08550"
     CDS             <1..1015
                     /locus_tag="PKY68_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH25117.1"
                     /translation="FRDQDMKMLKNHFPQIDKASGIISLYGSTVSYGKEYGNYGINGV
                     MPDYKDIRMLSIEAGNGRFINNIDMSEERKVVVINQRVADNLFRGEEPLGKYVQIGGI
                     AFMVVGLETKTSNDENGYCYIPHSTAQTIYNRRGYTDEFYFTVTGMDSPEANEAFTLA
                     LRKTLARSLIVHPDDTAAIGVWNMAENYVQTMRIFSTIQLFVFIIGLCTLVAGIVGVG
                     NIMVITVKERTREFGIKKAIGASPSNILNSILIESVIITGIFGYVGMVLGIGTLELLN
                     LIMENATGTGGDGPKVFLNPSVDLNIVLVATGILIASGLLAGYFPARKAVNIKPVEAM
                     RAE"
     gene            1027..2288
                     /locus_tag="PKY68_08555"
                     /pseudo
     CDS             1027..2288
                     /locus_tag="PKY68_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859309.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2321..>2673
                     /locus_tag="PKY68_08560"
     CDS             2321..>2673
                     /locus_tag="PKY68_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HOH25118.1"
                     /translation="MKKVIRIVLLVLLAAVVLWTFFFLWQKSRPQAVVYEIIEVEQRT
                     IEKKAVATGKVEPRNEIQIKPQIAGIIDEIYKEAGEMVRKEEVIARIKVIPDMVTLNA
                     AESRVKVAEVNLNQS"
BASE COUNT          766 a          615 c          634 g          658 t
ORIGIN      
        1 ctttagggat caggacatga aaatgctcaa aaatcatttt cctcagatcg ataaggcttc
       61 ggggataatt tccctttacg gcagtaccgt cagctacggc aaagaatacg gcaattacgg
      121 aatcaacggg gtaatgcccg actacaaaga catcaggatg ctcagcatag aagccggcaa
      181 cggtcgtttt atcaacaaca tagatatgtc ggaagaacgc aaggtggtgg taatcaatca
      241 acgggttgcc gataatcttt tcaggggaga agagccgctg ggaaaatacg tccaaatcgg
      301 aggcatagcc tttatggtgg ttggcctgga aactaagaca tcgaacgacg aaaacggcta
      361 ttgctacatc ccccactcca cggctcaaac catatacaac agaagaggct ataccgatga
      421 attttatttt acggttaccg gcatggacag ccccgaagcc aacgaagcct ttaccctggc
      481 cttgcgcaaa acccttgcca ggtcgctgat agtccatccc gacgacaccg ctgccattgg
      541 agtatggaat atggcagaga attatgtcca gaccatgcgc attttttcca ccatacaatt
      601 gtttgtcttc ataatcggct tatgtacctt ggttgccggc atagtcggag taggcaatat
      661 tatggttatt accgtaaagg aaagaacccg cgagtttgga ataaaaaaag ccattggagc
      721 cagcccctcc aatattttga attccattct gatagaatcg gtcataatca ccgggatctt
      781 cggctatgta ggaatggtgc tggggatagg aacactcgaa ctcctgaatc tgattatgga
      841 aaatgcaaca ggcacgggcg gagacggccc caaggtcttt ttaaatcctt cggtagacct
      901 gaacattgtt ttagttgcca ccggcattct gatagcatcc ggtttgctgg ccggttattt
      961 ccccgccaga aaagccgtaa acattaaacc ggtagaagcc atgagggcag aataagactt
     1021 agcgatatgt ttgatataga taaatggcag gaaatctgga ttaccatagg ccgaaataag
     1081 cttagaagtc ttctcacggc ttttggtgtg ttctggggca ttttgatgtt gacgctgcta
     1141 ctgggtgtca gcaacggcct gagcaacggt atgaaaagca gtgttcaggg cattgccacc
     1201 aattcctgtt ttttcttttc cggcaccacc tccgaaccct acgccggttt taaaaaagga
     1261 aggcgctggg atatccgtct ttcggacatt gaccttatac gttctcaggt tgaaggagtc
     1321 agagatgtgg cccctgtcgt ttttggaggc aggggcacgg acaatgtgat gtacggagac
     1381 aaactgggta gtttcaacgt cagaggaaac gggcctgaac tgatgcgcat tgaagagcaa
     1441 aatatgctct atggcaggta catcaacgaa atcgacatca gccaaaagcg taaagtttgc
     1501 gttatcggca agaatgtctt ttccgctttt tttaaagaag gcaccaatcc ctgcgggctc
     1561 ttgatcagat gcaacggaca atattaccgg atagtgggtg tcaccaaagg tgtatcgaac
     1621 aatatttctc tgggggacgc agcgacgata tggtggtttt gcccatcacc actatgcaac
     1681 aactctataa tcagggtgat gtaatccact ttttcggagt acgtgcccac gatcattacg
     1741 acatcaccaa gctggaagaa aacatcaaag acattctcaa aagccgccat cagatttcac
     1801 ccaccgatcc acaggcggtt gtatccataa acataggcga aatgtttaaa acattcatgt
     1861 atttgtttct ggggatttcc attttgacct ggatagttgg cgtaggcact ttgctggccg
     1921 gagccatagg cgtcagcaac attatgctgg tatcggtccg cgagcgcacc aaagaaatag
     1981 gtattcgccg ggccctggga gcaaaaccct ccaatatcat ttcacaaata atgacggaaa
     2041 gcctggtatt aactgccctg gccggatttg taggcttcac gctgggcgtg ggtatactcg
     2101 ccctgatcga tcagatcatt gccaacgcac cggccgatag cgacatcttt ttacaacaat
     2161 tgatgatcga tttcaaagtg ggaatttcag ctgcagccat tctcctgttt ttcggattac
     2221 tggccggcct gctccccgcc atgagagcca tgcaaattaa acccatagaa gctttaagcg
     2281 aagaataaat aaataataat catatcaaaa aacctccttt atgaaaaaag ttattagaat
     2341 cgttctgctt gtcctcctag ctgcagtcgt gctttggact ttcttttttc tctggcagaa
     2401 atcacgtcct caagcggtag tttacgaaat catcgaggta gagcaaagaa ccatcgagaa
     2461 aaaagccgtt gcgacaggca aggtagaacc tcgcaacgaa attcagatca aaccccaaat
     2521 tgccggtatc attgacgaaa tttacaaaga agccggcgaa atggtacgaa aagaggaagt
     2581 cattgcccgc attaaagtaa tccccgatat ggttacactc aacgcagccg aatcgagggt
     2641 caaagtggcc gaagtcaatc tgaaccagtc gca
//
LOCUS       DAOSGL010000312         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_37128_length_2656_cov_3.244906, whole genome shotgun sequence.
ACCESSION   DAOSGL010000312 DAOSGL010000000
VERSION     DAOSGL010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37128_length_2656_cov_3.244906"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKY68_08565"
     CDS             <1..358
                     /locus_tag="PKY68_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH25119.1"
                     /translation="ITDTLKVSGRHISVDAPFVEGEDNNLLDVLINDDSPLADRSLMN
                     ESLSLEIDRALSTLTERESEIVKMFFGLGCQEMTLEEIGDKFGLTRERVRQIKEKAIR
                     RLRSTSRSKFLKTYLG"
     gene            471..2564
                     /locus_tag="PKY68_08570"
     CDS             471..2564
                     /locus_tag="PKY68_08570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOH25120.1"
                     /translation="MNNNKIPILMAIAALSFSCSPKEGSRSKEIPLSKPQLELTGDLM
                     TPEILWSMGRLSDIQLSPDKKTVLYGVSYYSITQNKGNRELYLVDMEGKQRRQLTATA
                     VSESAAQWSADGKRIYYLSTVSGSSQLWQMLPDGRKPKKLTSVEGGIDAFRFSPDGKK
                     LLYVAQVKYGKRAVDYYPDLDKTTGRIIDDLMYKHWDEWVETIPHPFVADFSSKGLSN
                     VRDLLQDEPFESPMKPWGGIEHLAWSPDGKIVAYTCRKKTGVEYSRSTDSDIYFCFLE
                     ENFRTENITAELPGYDTNPVFSPCGGSLAFCSMSRDGYESDKNRLMVYELSTGKMKDL
                     TADFDQEANSVVWADDAKSLYFVSCWHGRVHIYQAFTDGSPIRQITGGDYDFADVHVA
                     GEYLVGLRHSLSQANEIIRIDPQSGEMLDLSRENKEIYDQLSLGRVEERWVKTTDNKE
                     MLVWVVYPPHFDSSKKYPALLYCQGGPQSPVSQFWSFRWNLQLMAANGYIVVAPNRRG
                     LPGFGQEWLEQISGDYGGQNMKDYLSAIDALSAEPFVDRDRLGCVGASYGGFSVYWLA
                     GNHEKRFKAFISHSGIFNLEQQYLETEEMWFADWDLGGPYWERDNKTAQLSYANSPHR
                     FVDKWDTPILCIHGEKDYRILASQGMAAFNAAILRGIPAELLVFPDENHWISKPQNAI
                     LWQRRFFAWLDKYLK"
BASE COUNT          727 a          571 c          653 g          705 t
ORIGIN      
        1 gattacagac actcttaagg tgtccggtcg tcatatttcg gtggatgctc cttttgtgga
       61 aggagaagac aacaacctgc tggatgtatt gatcaacgac gattccccac tggccgatcg
      121 ttcgctcatg aacgaatcgc tcagtctgga aatcgacaga gccttgtcaa ctttgactga
      181 acgcgagagc gagattgtta aaatgttttt tggtttagga tgtcaggaaa tgacgctgga
      241 agaaatcggc gataaattcg gtttgacccg ggagcgtgtc cggcaaatca aggaaaaagc
      301 catacggcgt ttacgtagca cttccaggag caaattcctt aagacttacc tcggataaaa
      361 ccaaacaaac ttaaagtatt gaaggtgtcg gtatactccg gcaccttttg tttttttaga
      421 aaagcggcat atctttgcta gtgattaact ttgtatttac attattagct atgaataaca
      481 acaaaatacc gatattaatg gctattgcgg cacttagttt ttcctgctcc ccaaaagagg
      541 gtagcaggtc caaagaaatt cctttaagca aacctcaact tgaattgacc ggcgacttaa
      601 tgactcccga aatattatgg agtatgggca ggttgagcga tatccagctt tctcccgata
      661 aaaagactgt tctgtacgga gtgagctatt acagtattac tcaaaacaag ggaaaccggg
      721 aactgtattt ggtcgacatg gagggaaaac aaagaaggca gcttactgct acggccgttt
      781 ctgaatcggc agcccaatgg tcggccgacg gcaagagaat ctattatctc tctacagtat
      841 cgggcagttc tcaattatgg cagatgctgc ccgacggcag aaaacctaaa aaactgacct
      901 cggtcgaggg aggcatagat gcctttcgtt tttcgcccga tgggaaaaaa ctgctctacg
      961 tggcccaggt aaaatacggc aaaagggcgg tagattatta tcccgattta gataagacca
     1021 ctggccggat tattgatgat ctgatgtata agcattggga tgaatgggta gaaactattc
     1081 ctcatccctt tgtagccgat ttctcctcca aaggtttaag caatgtgcgt gatttacttc
     1141 aagatgagcc ctttgagtct ccaatgaagc cttggggagg catagagcat ctggcctggt
     1201 cgcccgacgg gaaaatagtg gcttatacct gtcgcaaaaa aacaggtgtc gaatattcac
     1261 gttcgaccga ttcggatatt tatttctgct ttttggaaga aaactttcgt actgaaaaca
     1321 ttaccgccga attgcccggc tacgatacca atcccgtatt ttcaccttgc ggaggcagtc
     1381 tggccttttg cagtatgtcg cgcgatggct atgagtccga taaaaaccgt ctgatggttt
     1441 atgagctcag caccggtaaa atgaaggacc tcactgccga tttcgatcag gaagctaatt
     1501 ctgtggtatg ggctgacgat gccaaatcgc tctattttgt gagctgttgg catggcagag
     1561 tgcatattta tcaggcattt accgatggaa gtcccatcag gcaaattacc ggtggcgact
     1621 acgattttgc cgatgtacat gtggcaggcg agtatctggt cggtttaaga cattctctat
     1681 cgcaggccaa cgaaattatc cggatcgatc cccaaagcgg ggagatgctc gatttgagcc
     1741 gagaaaacaa agagatttat gatcagctca gtttaggcag agtggaagag cgttgggtta
     1801 aaaccaccga caataaagaa atgctggtct gggtagtgta tcctcctcat tttgactcct
     1861 ctaagaaata tcccgccctg ttatattgcc agggtggacc tcagagtccg gtcagtcaat
     1921 tctggtcttt ccgctggaat ctacaactga tggctgcaaa cggttacatc gttgtggctc
     1981 ctaaccgccg cggacttccg ggctttgggc aggaatggtt ggaacaaatc agcggagatt
     2041 acggcggtca gaatatgaaa gactaccttt cggccattga cgcgctgagt gcagaacctt
     2101 ttgtagaccg tgatcgtttg ggctgcgtgg gagcttcgta cggcggtttt tccgtctatt
     2161 ggctggccgg caaccacgaa aagcgtttca aagcttttat ttcgcacagc ggcattttca
     2221 atctggaaca acagtacctg gaaaccgaag agatgtggtt tgccgattgg gatttgggtg
     2281 gtccttactg ggaacgggat aataaaacag cccagctcag ctatgccaac tctcctcatc
     2341 gttttgtgga taagtgggat acacccattc tttgcattca cggcgaaaaa gattaccgta
     2401 tcctggcttc gcagggaatg gccgctttta atgctgcaat acttcgaggt atacctgccg
     2461 aactgttggt tttcccggac gaaaatcact ggatttccaa gcctcaaaac gccatattat
     2521 ggcaaagaag attttttgcc tggctggata aataccttaa atgagaaagt accgattatt
     2581 ttaatactcg caaatagtaa aatcatgaaa agagtagcct ttaaaatgaa aatgatcccc
     2641 ggcatgaagg aggagt
//
LOCUS       DAOSGL010000313         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_37290_length_2648_cov_2.739298, whole genome shotgun sequence.
ACCESSION   DAOSGL010000313 DAOSGL010000000
VERSION     DAOSGL010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37290_length_2648_cov_2.739298"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..666)
                     /locus_tag="PKY68_08575"
     CDS             complement(127..666)
                     /locus_tag="PKY68_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOH25121.1"
                     /translation="MSGENKLQWFAAKTRPHQELKIKNKLDGLGVENFLPMKEQIRVL
                     GEKKRKVMRPLVPRLLFLRCRKELLFALLNEHFLPLSYVRNLETKRPLVVPDQQMRDF
                     MFFVDFAQHSIQVLNDKLKPGDRVRVVKGDLAGLEGELIRIKGHKRVVVRLQGLFSVA
                     AGTYLPKHFLELVDKKSNS"
     gene            complement(1176..1248)
                     /locus_tag="PKY68_08580"
     tRNA            complement(1176..1248)
                     /locus_tag="PKY68_08580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1213..1215),aa:Thr,seq:cgt)
     gene            complement(1361..>2648)
                     /locus_tag="PKY68_08585"
     CDS             complement(1361..>2648)
                     /locus_tag="PKY68_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOH25122.1"
                     /translation="HTLNQYFKLSVTLFFFVGLVVLLLAETLGLWFVNTQMVIPAERI
                     RAANWVYQCSIISFMLNMFLVPFQSMIVAFENMKIYAFAGIIEVILKLLVIYFLLVVN
                     ADKLILFAVLYLSISLVIFVLYVVYCLRKYPSCAYSFFFDKSMFKEFSSYSGWIIFGS
                     LAGVIRGQGINIVLNLFFGPIVNAARGIAFQVERAVSLFVHNFYTAVRPQITKRYSAG
                     NFKDMFDLVFSSSRYCYYLVFVLGIPILIQTEYILQLWLGEAPAMSVLFTRLVLIAAV
                     VESLSYPLDTAVMSTGRINAYQFITGGLLILNLPLSYLLLKLGYPPESTMYVAIAMAL
                     AAHIPRMLYAKKEAGLSLWAYTREVLWYISLVTVVAVLPLLFLYRKINFGGFGLFVVF
                     SLLCVLWSLLVIYFIGIGKRERKQLAGFVLQKIRQK"
BASE COUNT          787 a          618 c          610 g          633 t
ORIGIN      
        1 ccaataactg aggtaacgcg acgatagtat tcgcttgagt attttttcga agagattctt
       61 catatacgat gaatgtgttt aaccaaggaa gtttttggtt gatttgaact atacacgctt
      121 gttaaactat gaattacttt ttttgtcaac aagttccaga aaatgcttgg gcagataagt
      181 gccggcagcc accgagaaca ggccctgcag gcgtaccacc acccttttgt gtcctttgat
      241 gcgtatcagt tcgccttcga ggccggccag atcgcctttg accacgcgta cccggtcgcc
      301 gggcttcagt ttgtcgttga gcacctgtat ggagtgttgg gcaaaatcga caaaaaacat
      361 aaaatcccgc atctgttggt cgggaactac cagtggacgt ttggtttcga ggttgcggac
      421 gtaactgagg ggcagaaaat gttcgttgag caaggcgaaa agcagctctt tgcgacagcg
      481 cagaaacagc agccgtggca ccaaaggccg catcacttta cgtttttttt cgcccaaaac
      541 ccgaatctgc tctttcatgg ggagaaaatt ctccacaccc agcccgtcca gcttgttttt
      601 gatcttcagc tcctgatgcg gcctggtttt ggcggcgaac cactgcaatt tgttttctcc
      661 tgacatgtac tgaatcttgt acgtatttcg gaaatgggcc gctgtgtata aagcctgaat
      721 ccggacgttt attgccccta atccggcttt aatctaaaga aagcttaaca aaacccaaag
      781 cttcgccgac tatttccaat acagaaatca gcaagtcggt tttttaactc agggttgaag
      841 cagccactta cacagcaagc atacctctta ccaaacgccg gcaaaggtaa tatattttac
      901 tgtatagaca caaaaataac aggaaaatca atcaattttt attaacacaa acaggcgatc
      961 ctgttaacaa tttcaggaag cagctgtatg tacataaaaa agcaggcctg aaactactgt
     1021 tcaaacctgc tttccgcctg tagtttgcaa gctccggaga gcctgaaatc gatgaacttt
     1081 gggttatgaa aacccttgct tgagcgccct taaaaaaggc cgaacaccaa acaattttct
     1141 gagccttttt gagccccttt cgtcttattc ttctgagccg aaggccggac tcgaaccggc
     1201 gacctacgca ttacgaatgc gttgctctac caactgagct atttcggcaa aaccgctgca
     1261 aaagtacaag cttttgccga caggacaagt atctttttga aaaaatttac tcataggctt
     1321 ttgggaggcc ggacaaatag tcatacaaag gcagccggtt ttatttttgt ctgatttttt
     1381 gcaaaacaaa acctgccagc tgtttacgct cccttttgcc gatgccgata aaataaataa
     1441 ccagcaaaga ccacaggacg cagagcaggc taaagacgac aaaaaggcca aagccgccaa
     1501 aattaatttt ccgatacaaa aagagcaaag gcaaaactgc taccacagtt accagactaa
     1561 tgtaccataa gacttcgcgg gtataagccc acagcgaaag gccggcttct ttttttgcat
     1621 acaacattcg cggaatatgc gctgccagag ccattgctat ggctacatac atggtgcttt
     1681 cggggggata acccagcttg agcaacagat acgacagggg caaattcaat atgagcaggc
     1741 cgccggttat aaactgatac gcattgatcc tgccggtgga cataaccgcc gtatccaggg
     1801 ggtaagagag cgattctaca acggcggcaa tcagcaccag cctggtaaag agcaccgaca
     1861 tggcgggtgc ctcgcccagc cagagctgca ggatgtattc ggtctgaatg agtatgggga
     1921 tacctaacac aaaaaccagg taataacaat agcgcgagga gctgaacacc agatcgaaca
     1981 tgtctttgaa gttgccggcc gaatagcgtt tggttatttg aggcctcaca gcggtgtaaa
     2041 aattgtgcac aaagagcgat acggctctct ccacctgaaa agcgatacct ctggccgcat
     2101 tgacaatagg gccgaaaaac aggttaagta cgatgttgat gccctggcca cgtattactc
     2161 cggccagcga accaaatata atccagcccg agtaggagct gaattctttg aacatgcttt
     2221 tgtcgaagaa aaaagagtaa gcacacgagg ggtatttacg taggcaatac acaacatata
     2281 acacaaagat caccagcgag atgctcaagt acaaaacggc aaagagtatc agcttgtcgg
     2341 cattaactac cagtaagaaa tatataacca ggagttttag tatgacctcg attatgcccg
     2401 caaaggcata gattttcata ttttcgaagg ctacaatcat cgactggaag ggcaccagaa
     2461 acatattgag cataaaggag ataatggagc actggtagac ccagttggcg gctctgatcc
     2521 gctcggcggg tatcaccatt tgggtattga caaaccataa gcccagggtt tcggccagca
     2581 gaaggacgac gaggccgaca aaaaagaaca gggtgactga aagcttgaaa tactgattca
     2641 atgtgtgt
//
LOCUS       DAOSGL010000314         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_37365_length_2644_cov_3.994206, whole genome shotgun sequence.
ACCESSION   DAOSGL010000314 DAOSGL010000000
VERSION     DAOSGL010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37365_length_2644_cov_3.994206"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..>2644
                     /locus_tag="PKY68_08590"
     CDS             92..>2644
                     /locus_tag="PKY68_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOH25123.1"
                     /translation="MKTIRPLILGLLLISASAAAQTTVTATWPFDRGTDNPSSAQIDN
                     DTAFTLSNFVLGSHLTCTGIQSPNTESVSYSKIQPDAQMGSATPGYELSFMLKPSRGL
                     QFTPTRISFKASRFGTDGGFLVLTRATGEGAEETIAQDLKPNRNNVDPFFSVYDYALE
                     GLPASEDAFVFKIYIHSLGNNKQIGFYNIAITGRITGELIEVNKYTLNTAVSPAAAGS
                     ITLSPQGNSFDEGSEITLTANKAFGYKFNHWEDGQGTVLSQENPYVHTITADTNLIAV
                     FDKLNTYSLMLAVEGGANDYMLSVSPAGTLVNDTLRYEENTQVSLKASGNKILTFNNW
                     SSGETNSELLLDMTRDYELTAYYSAVDFIVGWDFYRPGNSGRPADFASEGNSTSILIL
                     RNAEGNATGWLDKSQQAAGGYEGRPSAVNWKALADKYYYQLSFDATNFTDIKVASALL
                     FNYNAYSKIKLEYSLDNENFTTVDTIHMLSNKVWYDTEFSLPSDADHAPKVYVRWLPD
                     YTSPVLGTTSNNDGTSITDIYITGTGLIYDDGKAPVFLGSVPADQSTGASATGKIVLS
                     FDEKIKLSDTLYATLDDQPLEGLVSGKTLSFAYRGLEYAREYTFVLPANAVSDLTDNF
                     LDSVITIRFTTMERPVVAKKLFDAVVSNTGELLAALEAAHASQTGERFRILLKNGEYD
                     LGSGITTITAHKVSLIGQSREGVLVYNTSINEGISITSTIFLSGATGFYAQDITFQNK
                     YPYVDATGRAVVLRDTGTKNIFKNISLLSFQDTYYSGNSGRMYFEDCQINGVVDFICG
                     GSDIFFNRCLLALEKRSGNCITAPSGNGDWGYVFSDCTIDGVDDEARAVVNGT"
BASE COUNT          653 a          706 c          642 g          643 t
ORIGIN      
        1 aactatttga tacgattaac aaccgatctt tcttgttttt tctaaaaaag agttactttc
       61 gcctctttat tagccttatt tactaacaga tatgaaaaca atcagacccc ttattctggg
      121 cctcttgctg atctcggcat cggctgcggc ccaaaccacg gtaacggcca cttggccttt
      181 tgaccggggt acagacaatc cttcgtctgc ccaaatcgac aacgatacgg catttacatt
      241 gagcaatttc gttttgggtt cacacctgac ctgcacaggc atacagtcgc ccaataccga
      301 aagtgtatcg tattccaaaa ttcagcccga tgcccagatg ggttcggcca ccccgggcta
      361 tgagcttagt tttatgctca aaccctcgag gggattgcag tttacaccta cccgcattag
      421 tttcaaggct tcgcgctttg gcaccgacgg aggttttttg gtgttgacca gggccaccgg
      481 cgaaggggcg gaagaaacga tagcccagga tttaaaaccc aaccgcaaca acgtagaccc
      541 cttcttttcg gtgtacgatt atgccctgga aggtctgccg gcttcggagg atgcttttgt
      601 attcaagatt tacatacaca gcctgggaaa caacaagcaa atcggcttct ataacatcgc
      661 cattacaggc cggattacgg gcgaactgat cgaagtgaac aaatatacgc tgaacacagc
      721 cgtttctcct gccgcagccg gcagcataac gctgagtcct cagggaaatt ccttcgacga
      781 aggttccgaa atcaccctga ccgccaacaa ggctttcggt tacaaattca atcattggga
      841 agacggtcag ggtaccgtcc tgtcgcaaga gaatccctat gtgcatacca ttacggccga
      901 taccaacctg attgccgttt ttgacaaact caatacctac agcctgatgc tcgctgtgga
      961 gggcggcgcc aacgattaca tgctttccgt ttcgcctgca ggcacccttg tcaacgatac
     1021 ccttcgttac gaagaaaata cacaggtgag cctcaaagct tcgggaaaca aaatcctgac
     1081 cttcaacaat tggagctcgg gcgaaaccaa cagcgaattg ctgctcgaca tgactcgcga
     1141 ttacgagctg acggcttact acagcgcagt ggatttcatt gtgggctggg atttttaccg
     1201 tcccggcaac agcggccgtc ccgccgattt tgcctccgaa ggcaacagca cttccatcct
     1261 gatactccgc aacgccgagg gtaatgccac cggctggtta gacaagtcgc agcaggccgc
     1321 cggaggttac gaaggccgcc cctcggcagt gaactggaaa gctctggcgg acaagtatta
     1381 ttaccagctc agcttcgacg ctaccaattt cacggatata aaagtagctt cggcgctttt
     1441 gttcaattac aatgcctatt ccaaaatcaa actggaatat tcgcttgata atgagaattt
     1501 tactactgta gataccattc atatgttgag caataaggta tggtacgaca ccgaattcag
     1561 tctaccctcc gacgccgacc atgcacccaa ggtgtatgtg cgctggctgc ccgattatac
     1621 ttcgcctgtg ttgggcacta catccaacaa cgacggcact tcgattaccg atatttatat
     1681 cacgggaaca ggcctgatct acgacgacgg caaagctccg gttttcctgg gttcggttcc
     1741 ggccgaccaa tcgaccgggg cttcggcaac cgggaaaatc gtactcagtt ttgacgaaaa
     1801 gatcaagctg tccgatacct tgtatgccac tctcgatgac caacctctgg aaggcctggt
     1861 cagcggtaaa accctgagtt ttgcttacag gggcctggag tacgccaggg aatacacatt
     1921 tgttctcccc gccaacgcgg tatcggacct gacggataat ttcctcgatt cggtgattac
     1981 catccgcttt accactatgg aacgtcctgt ggtcgctaag aagcttttcg atgccgtggt
     2041 gtccaatacc ggggaattgc tggcggcact tgaggcggca catgcttcgc agacgggcga
     2101 acgtttccgc atcctgctca aaaacgggga atacgacctg ggttcgggta tcactaccat
     2161 cacggcccac aaggtttcgc ttatcggaca gagccgcgag ggggtcctgg tgtacaatac
     2221 ttccatcaac gagggcataa gtattacttc caccattttc ctgagtggag ccacgggatt
     2281 ttatgcccag gacattactt tccaaaataa atatccttac gttgatgcca cgggccgggc
     2341 agtggtgttg cgcgataccg gcacgaagaa tattttcaaa aacatcagtc tgctcagttt
     2401 ccaggatacc tattattcgg gcaattcagg ccggatgtat ttcgaagatt gccagatcaa
     2461 cggggtagtc gactttattt gcggcggcag cgatattttc ttcaaccgtt gtctgctcgc
     2521 tttggaaaaa cgcagcggca attgcattac ggctccttcg ggcaacggcg attggggcta
     2581 tgtctttagc gattgcacca tcgatggcgt agacgacgaa gccagagcgg tggtgaacgg
     2641 cacg
//
LOCUS       DAOSGL010000315         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_37409_length_2642_cov_4.488210, whole genome shotgun sequence.
ACCESSION   DAOSGL010000315 DAOSGL010000000
VERSION     DAOSGL010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37409_length_2642_cov_4.488210"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PKY68_08595"
     CDS             <1..712
                     /locus_tag="PKY68_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOH25124.1"
                     /translation="LQESEADVLCLQEAGYSFDKKLLEKTTVITALKDYPYHSLEIIQ
                     SNKYRTSNLWVFSKYPILKSGRLAYKSRSNASHYCDIKIGQDTLRFINNHLESNKLTK
                     EDMALYKIIVENPDRDNISQVAEKLGRKLGQAARLRAPQAETIAGFIRETPYKLVVCG
                     DFNDIPASYTYRCIKKGLRDAWSQNGRGLGITFHEKFYLFRIDYILHSPELRSYGTKV
                     DHVRYSDHYPVWTYLELP"
     gene            709..>2642
                     /gene="priA"
                     /locus_tag="PKY68_08600"
     CDS             709..>2642
                     /gene="priA"
                     /locus_tag="PKY68_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOH25125.1"
                     /translation="MKALYADVILPLAFGSGLSYSVPESLAENIRPGCRVRVPLGKQK
                     MYTGIVARLFDVPPTDINLKEIAGLIDDAPVVNAVQLEFWQWLFSYYLCTPGEVMKAA
                     LPAGLKGPRVAKAKTKSFLCLQPSLRDNPPAIEQTLTALARAKKQQEALRVFLRLAQT
                     SEHGPGLFREQLIEAGQQAAIIKALIQKNILEVHEIEVLPDRVPAEALRAAKQLSREQ
                     QKAMETIRQGWESGTNVCLLHGVTSSGKTEIYIHLILDYLAQGKQVLYLLPEIALTTQ
                     LTARLTEVFGNRLGIYHSACSDSERTHLWNKQLGDDPFDIILGVRSSVFLPFKNLGLV
                     VVDEEHENSYKQQDPAPRYHARNAAIVLASLHGAKTVLGTATPSIESYYNCRTGKYKL
                     VELSSRYLDWQLPEVMAVNTAELKRKKRMKSLLSPPLIAKMQETLEAGQQIMLFRNRR
                     AYALMLECAACGWVPRCKRCDVSLAIHKQGSVLRCHYCGKKYNRPQICPSCGGSDFEL
                     RGYGTEKVEEEVRRLFPAIRIARMDSDTMRNRAAYEKTIADFEQHKIDVLIGTQMLTK
                     GLDFDRLQLVGILNADSMLNYPDFRAHEHAFQLMTQVSGRAGRKSGRGSVIVQTSDPQ
                     HPVIQYVLNNDYRAFFQKEC"
BASE COUNT          711 a          635 c          648 g          648 t
ORIGIN      
        1 tttacaagaa agcgaagccg acgttctttg cttgcaggag gccggttaca gcttcgataa
       61 aaaactcctg gaaaaaacca ctgttattac tgcactcaaa gactatccct accacagttt
      121 ggaaatcatc caatctaata aatacagaac ctccaatttg tgggtctttt caaagtatcc
      181 gattttgaaa tccggcagat tagcctacaa gagccgatct aatgcttcac actattgcga
      241 cattaaaata ggacaagaca ccctgcgttt tattaacaat cacctggaat caaacaaatt
      301 aaccaaagaa gacatggctt tgtacaaaat cattgtggaa aatcccgaca gagacaatat
      361 aagccaggtt gccgaaaagc taggtcgaaa attgggtcag gcggccaggt tgcgagcccc
      421 tcaggccgaa accatcgccg gttttattcg ggaaaccccc tataaactgg tggtttgcgg
      481 tgattttaac gatattccgg cttcttatac ctaccgctgc attaaaaaag gcttgcgcga
      541 tgcctggtcg caaaatggcc ggggccttgg aattactttt catgaaaagt tttatttgtt
      601 caggatagat tatattctgc attccccgga gttgcgatct tacgggacca aagtggatca
      661 cgtacgttat tccgatcatt accctgtttg gacttacctg gaattgccat gaaagctctg
      721 tatgccgatg tgatactgcc tttggccttt ggatcgggtc tgagttacag tgtgcctgaa
      781 tctctggcgg agaatatccg gccgggatgc agagtaagag tgcccctggg taaacaaaaa
      841 atgtacaccg gcatagtagc ccggcttttc gatgtgccac ccactgacat caatctgaaa
      901 gaaattgcag gccttatcga cgatgccccg gtggtaaatg ccgttcagct ggagttttgg
      961 caatggttgt tttcgtatta tctctgcacg cccggcgaag tgatgaaagc cgctctaccg
     1021 gcgggcctta aaggcccccg ggttgccaaa gccaagacta aaagtttctt atgcctgcag
     1081 ccttcgctca gggataatcc tccggcgata gagcagacac tgacagcatt ggcaagagca
     1141 aaaaaacaac aggaagctct tagagttttt cttcgtttag cccaaacttc agaacatgga
     1201 cccggtttat tcagagagca gcttatagag gccggccagc aagcggctat tataaaggct
     1261 ttgatccaaa agaatatact cgaggttcac gaaatcgaag tgttgccgga tagggtgccg
     1321 gcggaggctc tgagagctgc caaacagctc agccgggaac agcagaaagc tatggagacc
     1381 atacggcaag gctgggagag cggcaccaat gtctgccttc tacatggcgt gacctcttcg
     1441 ggtaaaaccg agatatacat tcatctgata ctggattacc tggcgcaggg taagcaagtg
     1501 ctctacctct tgcccgaaat tgctcttact actcagctta ccgcccgctt aacagaagtg
     1561 ttcggcaatc gcctggggat atatcactct gcttgcagcg acagcgaaag gactcatctt
     1621 tggaacaagc aactcggaga cgatcccttc gatattattc tgggagtgcg ttcttctgtt
     1681 tttttgcctt tcaaaaacct gggactggtc gtagtggacg aggaacatga aaacagctat
     1741 aaacaacaag atccggctcc ccgttatcac gcccgtaatg cagccatagt gctggcctct
     1801 ttgcacgggg caaaaacagt gcttggtacg gcaacaccca gcatcgaaag ttactacaat
     1861 tgccgtacgg gcaagtacaa actggttgaa ctgtccagca gatacctcga ttggcaattg
     1921 cctgaagtta tggcagtgaa taccgctgaa ttgaagcgca agaaaaggat gaaatcgttg
     1981 ctttcgcctc ccctaatagc caaaatgcag gaaacgctcg aagccgggca gcaaatcatg
     2041 ctttttcgca accggcgtgc ttacgctctt atgttggaat gtgctgcctg cggctgggtg
     2101 ccgcgttgca agcgttgcga tgtgagcctg gccatacata aacagggttc ggtattgcgt
     2161 tgccattatt gtggcaaaaa atacaaccgg ccgcagattt gtccttcctg cgggggctcg
     2221 gattttgaat tacggggtta tggcaccgaa aaggtcgaag aggaagttcg ccgtttgttt
     2281 cctgcgatac gcattgcccg tatggattcc gatactatgc gcaatcgtgc tgcctacgaa
     2341 aaaacgatcg ccgattttga gcagcacaag atagacgtat tgataggcac ccagatgctg
     2401 accaaaggtc ttgatttcga tcgtttacag ttggtgggga ttctcaacgc cgactccatg
     2461 ttgaattatc ccgatttcag ggcccacgaa catgcttttc agttgatgac ccaagtaagc
     2521 ggacgcgccg gccgaaaaag cgggagaggc agtgtaatcg tccagacctc cgatcctcag
     2581 caccctgtaa tacaatacgt tttgaacaac gattaccggg ctttcttcca aaaagaatgt
     2641 ga
//
LOCUS       DAOSGL010000316         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_37469_length_2640_cov_2.822824, whole genome shotgun sequence.
ACCESSION   DAOSGL010000316 DAOSGL010000000
VERSION     DAOSGL010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37469_length_2640_cov_2.822824"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2610
                     /locus_tag="PKY68_08605"
     CDS             <1..2610
                     /locus_tag="PKY68_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOH25126.1"
                     /translation="REGITAPPPPWISNGKTEIDGFIGSDHQGQELMLANTSGAEVEL
                     KGQVSPASVQVNSPGDYRLYGTGELQGPMTLHKQGLGNLTIEGAQTYSGNTEVWDGLL
                     TLKGSLAGPLLLRRFAELDAYNSLEGGLQMEYGAILRPGGSGKQSALRLKSIKMDKGA
                     VVEFNLSDADFACDTLLIQENLELSEGAVFRFVKPTPDSKPQPGDYLFAVCEGIVTAE
                     LNKIVIEGLPGVACSVLNQNDSLFLHVDEQRAAQLVYWHGKFDQGIWNLNNTRNFYIG
                     QDTVEFVTNDTVVFDQNTSSRAVNITEEVIPSEVRIQGDLNYAITGNGGIGGSSSLIK
                     EGQGKLSLLNTNPYTGKTLIKGGTVEVSSLATSQGAGSLGALSSVSNNLTIQNGAILK
                     TTGNISMESPMYLGPEGGCLDISNTLTMKGVISGSTLIKRGNGTLEVTAVNTHRKTIL
                     EAGTLKVLEEPNLINGYFGDTLVLNGGVVICLDGNSSYSRANWNIVVPAGKSATIYLD
                     GRCEYHGSLHGSGTLTLSAPYVRNYLCGNWSAFEGVVKATTKANSSYTPSFDFKNTYG
                     LPKATLDVAAGTTVTNQSGSFKLGAVSGNGTLGGSNAWEIGYLNTTNSFSGTITSALT
                     KVGTGTLLLSGANNYTGDTNINGGILRVNNANNATVSATGTGRLNINNGGTLTGRGFI
                     DNNIVYINSGGLFIPGAYYVGELKVDGSIYQNPGGVLEFRLNSATSHSSIKGLNVMTL
                     KGTLRILLKEGYTPSLGDSFELWSCNSFSSSIPELVLPELPANLAWDTSELFTGRGKL
                     SVTDANGLQLNSWDEMLDIQVVNINGLTVASLESKATEWEKDVKELNLAKGIYLIKLQ
                     SGKSSQIVKHYQP"
BASE COUNT          728 a          735 c          670 g          507 t
ORIGIN      
        1 agggaaggta ttaccgctcc ccctccccct tggataagca acggtaaaac ggaaatcgac
       61 ggttttatcg gcagtgatca ccaggggcaa gagcttatgt tggccaacac ctcgggagca
      121 gaagtcgagc ttaaggggca ggtcagtccc gcctctgtgc aggttaattc ccccggagac
      181 tacagactct acgggacagg ggaactgcaa ggccccatga ctctgcacaa acaaggactc
      241 ggcaacctca ccatcgaagg agcgcaaacc tacagcggga ataccgaagt ctgggacggc
      301 ttgcttacac tcaaaggcag cttagccggt cctctgttgc tgcgtcgctt cgcagagttg
      361 gacgcctaca actccctgga aggaggcctg caaatggagt acggggccat tttgcgcccg
      421 ggaggttccg gcaaacaaag cgccctacgt ctgaagtcca taaaaatgga caagggagcc
      481 gtggtagaat tcaatctttc cgacgccgat ttcgcctgcg acacgctgct tatccaggaa
      541 aacctggagc tgagcgaagg cgctgttttc cgttttgtaa aacctacacc ggactctaaa
      601 ccacagcccg gagactatct atttgccgta tgtgaaggca tcgttacggc cgaactgaac
      661 aaaatcgtca tcgaaggcct gcccggcgtt gcctgctcgg tactaaacca aaacgacagc
      721 ctgtttttgc acgttgacga acaacgcgcg gcacaattgg tctactggca tggtaaattc
      781 gatcagggga tttggaatct gaacaatacg agaaactttt acatcggaca ggatacggtg
      841 gaatttgtca ccaacgacac cgtggtcttc gaccaaaata caagctccag agcggtaaat
      901 atcaccgaag aagtcattcc ttcggaagtt agaattcaag gcgatttaaa ctatgccata
      961 accggtaatg gcggcatagg cggaagctcc tcgctcatca aagaaggcca ggggaaactc
     1021 agcctgctca ataccaatcc ctataccggc aaaaccctta tcaaaggggg aacggtagaa
     1081 gtcagttcgc tggccacttc gcagggggcc ggctcgctgg gcgccctgag ttcggtgtcg
     1141 aacaacctga ccattcaaaa cggcgcgata ctgaaaacca ccggcaacat cagcatggaa
     1201 agcccaatgt atctgggacc cgaaggcggt tgtctggaca ttagcaacac cctgaccatg
     1261 aaaggagtta tttccggcag caccctgatt aaacggggca acggcaccct cgaagtaact
     1321 gcagtgaaca cgcacagaaa aaccatcctc gaagccggca ccctcaaagt gctcgaagag
     1381 cccaatttaa tcaacggcta ttttggcgac accctggtct taaacggcgg agtggtgatc
     1441 tgcctggatg gaaacagttc ctacagcaga gccaactgga atatagtggt acccgccgga
     1501 aaatcggcca ccatctacct ggacggacgc tgcgaatacc acggatccct gcacggcagc
     1561 ggcacactga ccttgagcgc cccctatgtc aggaattatc tttgcggcaa ttggtcggct
     1621 ttcgagggag tggtcaaagc caccactaag gctaactcga gctacactcc ttcttttgat
     1681 ttcaaaaaca cctacggttt acccaaggcc acgctcgacg tagccgccgg caccacggtg
     1741 accaaccaaa gcggaagctt taagctcgga gccgtcagcg gcaacggaac cctgggcggt
     1801 tctaacgctt gggaaatcgg ttacctgaac accaccaata gtttcagcgg caccatcacc
     1861 tctgctctga ccaaagtggg cacaggcacc ctgcttcttt ccggagccaa caactatacc
     1921 ggagacacca acatcaacgg aggcatcctc agggtaaaca atgccaacaa cgctaccgtc
     1981 agtgccacag gaaccggccg gctgaacatc aacaacggag gcacgcttac cggcagagga
     2041 tttatagaca acaacatcgt ttacataaac tccggaggcc tgttcatacc gggagcctat
     2101 tacgtgggcg aactgaaagt ggacggatcc atttatcaaa acccgggagg agtgctggaa
     2161 ttccgactta actcggctac ctcccattcg agtataaaag gtctgaatgt aatgactctg
     2221 aaaggtacgc tgcgcatact gctcaaagag ggttacacac cttctctggg cgacagtttt
     2281 gagctttggt cctgcaatag cttcagcagt agtattcccg aactggtctt gccggaattg
     2341 ccggccaacc tggcctggga caccagcgaa ctctttaccg gcagaggcaa actcagcgta
     2401 accgatgcca acggcctgca gctcaatagc tgggacgaaa tgctcgacat acaggtggtc
     2461 aacatcaacg gactcaccgt cgccagcctg gaaagcaagg ccaccgaatg ggaaaaggat
     2521 gtcaaggagt taaacctggc caaaggcatt tacctgatca aactccaaag cggcaaaagc
     2581 agccagatcg tcaagcacta ccaaccctaa gccggcttga gtcttaaaac aaagaagcaa
//
LOCUS       DAOSGL010000317         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_38096_length_2612_cov_3.697693, whole genome shotgun sequence.
ACCESSION   DAOSGL010000317 DAOSGL010000000
VERSION     DAOSGL010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38096_length_2612_cov_3.697693"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKY68_08610"
     CDS             <1..232
                     /locus_tag="PKY68_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25127.1"
                     /translation="AIAGKKYYIKSEAVEQGRDSMFLVAGTNNSLTLETMTSAQALAD
                     DRAAWYFHFDPATQYYYIRNAATGKHIGLNAA"
     gene            254..1285
                     /locus_tag="PKY68_08615"
     CDS             254..1285
                     /locus_tag="PKY68_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH25128.1"
                     /translation="MDSTQAVVEKLRIQLIRNRNDIMSGDEQAWMNLSGFWIINPRGR
                     SSQSLRAFAQSIYSYNTTLVDASSTLRWILMDEEETHQFDRIIQGQGVFDPDTSLVYT
                     IKPAKMDVRLKAYVTNNVHLVNLREDDQRQKWKLQAVESQEEGAKRYYLYNLGAQKCL
                     TIQKWIGEETSTAQALRNTKMLAKDPENIHQQLSLKSVEVDFSGEVPVRYITIENDHP
                     VMLDTVLDERAFRLEVLNETVNSQVGNRFDVDGVRDTQRWILIADQVQTALPEASVAK
                     ADLFYRDGLLHYRLPENSTLEVFTLTGQLVAKTQAGMDEGELELDKGIYMVRVRQQQG
                     TEVFKILAH"
     gene            complement(1332..2390)
                     /locus_tag="PKY68_08620"
     CDS             complement(1332..2390)
                     /locus_tag="PKY68_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOH25129.1"
                     /translation="MITESKKDVIDIIHKVNQHWQAEHPYTVRAFWDNAAYHTGNMEA
                     YFLTGNEAYREYSQKWAEHNEWKGAKSTDKSQWRYHYGENDLYVLFGDWQTCFQTYID
                     LYRIEPEEIKIERAKEVMYYQMSTENRDYWWWSDGLYMAMPVMTKLYKLTGDELFLEK
                     MQEYYQFAEELMYDEEYALFFRDAKYVYPKEPTINGKKNFWSRGNGWVFAAYAKVMQD
                     LPEDNAFRKKIEQRFIAMAASLKKIQQPEGYWTRSLLDPEHAPGPETSGTAFFTYAYF
                     WGINNGLLDKKEYLPTAAKAWSYLSKTALQEDFTIGYVQPIGERAIPGQTVDARSTAN
                     FGVGAFLLAACELARYLD"
BASE COUNT          652 a          596 c          661 g          703 t
ORIGIN      
        1 cgctatagcc ggcaaaaagt actacatcaa aagcgaagct gtggaacaag ggcgcgattc
       61 gatgtttctg gtggccggca caaacaattc gctaacgctt gagacaatga cttctgctca
      121 ggccctggca gacgaccggg ctgcttggta tttccatttc gacccggcca cacagtatta
      181 ttatattcgt aatgccgcaa caggcaaaca tattggtttg aatgccgctt aggcctataa
      241 tggctttaca ctcatggaca gtactcaggc tgttgtagaa aaacttcgca ttcagcttat
      301 ccgcaaccgt aacgatatta tgagcggtga cgaacaagcc tggatgaatt tatccggttt
      361 ctggatcatc aatcccaggg gaagatcatc ccaaagcctg cgcgcttttg ctcaatccat
      421 ttattcctac aataccactc tggtcgacgc tagttctacg ttgcgctgga ttttgatgga
      481 cgaggaggaa acccatcaat tcgatagaat cattcaagga cagggcgttt ttgatcccga
      541 cacttccctg gtttacacta tcaagcctgc taaaatggac gttcgcctca aggcctatgt
      601 caccaacaat gtgcatttgg tgaacctgcg ggaggacgat caaaggcaga aatggaaact
      661 gcaggctgtc gaatctcagg aagagggagc gaaacgttat tacctataca acttaggcgc
      721 ccaaaaatgc ctgaccatac aaaagtggat aggggaagaa acttccacgg ctcaagccct
      781 gagaaacacc aagatgctgg ctaaagatcc ggaaaacatt catcagcaac tgagcctgaa
      841 atcggtcgaa gtggactttt cgggagaggt gcctgtgcgc tacatcacca tagagaacga
      901 tcacccggtt atgctcgata ctgtgctcga cgaaagggct ttccgtctgg aagtactcaa
      961 cgaaactgtt aactcgcagg tgggcaatcg tttcgatgtg gacggagtac gtgacactca
     1021 acgctggatt ctgattgccg atcaggtgca gaccgccttg cccgaagcaa gcgttgcgaa
     1081 agccgatttg ttttaccgcg atggattatt gcactatcgt ctgcccgaaa acagcacact
     1141 ggaagtcttt acgctgaccg gacagttggt ggcgaaaact caggccggca tggacgaagg
     1201 agaattagaa ctcgacaagg gcatttatat ggtcagagta cgtcagcagc aaggtaccga
     1261 ggtctttaaa attcttgcgc attgattttc ttgcgcatta attcatgtaa tctgcccgac
     1321 cgggtaagaa attaatccag gtaacgagcc agttcgcagg cagcgagcaa aaaggctccg
     1381 acaccgaaat tagctgttga ccgggcgtct accgtttgtc ccggtatggc tctctctcca
     1441 atgggttgca cgtagcctat agtgaagtct tcttgcaaag ctgttttgct gaggtatgac
     1501 caggctttgg cggctgtggg caggtattct tttttgtcga gcaggccgtt gttgataccc
     1561 cagaaatacg cataggtaaa gaaggccgta ccactggttt cgggccccgg tgcatgttcg
     1621 ggatcgagca ggctgcgcgt ccagtagcct tcgggttgct ggattttttt caggctggcg
     1681 gccatggcta taaaacgttg ctctattttt tttctaaagg cgttgtcttc gggcaaatcc
     1741 tgcatgactt tggcatatgc ggcaaacacc cagccgttgc ccctggacca gaaattcttt
     1801 ttgccgttga tggtgggctc tttggggtag acgtatttgg catcgcggaa gaaaagagca
     1861 tattcctcgt cgtacatgag ttcttcggcg aattggtaat attcctgcat tttttccaga
     1921 aagagctcgt cgcccgtcag tttatacagt ttagtcataa cgggcatagc catgtaaagg
     1981 ccgtcggacc accaccagta atccctgttt tcggtgctca tttggtaata catcacttct
     2041 ttggcccttt ctattttgat ctcttcgggt tcgatgcggt agaggtcgat ataagtttgg
     2101 aagcaggttt gccaatcacc gaaaagcacg tagagatcgt tttcgccgta atggtatctc
     2161 cactggcttt tatcggtcga tttggctccc ttccattcat tgtgttccgc ccatttttgg
     2221 gaatattccc tgtaggcttc gttgcctgtc aggaaataag cttccatgtt gccggtgtgg
     2281 taggcggcgt tgtcccagaa ggcacgcacc gtgtaggggt gttcagcttg ccagtgttgg
     2341 ttgactttgt gaataatgtc gatgacgtct tttttgcttt cggtgatcat atcttttttg
     2401 gaggcgcaag cgctcaaact taagagggtt aaacaaaatg caaacgaaaa tagcttcgtc
     2461 gggagagaaa atttcatgtg agtaaggaaa aataattacg cttgcgaagg aacaaaaaat
     2521 ccatgttatt cggttctaaa aatcgttcat aatggtgcaa acttttgttc aaagaatgct
     2581 tgtgggcatt tgtatcattg cgtatcccgg tt
//
LOCUS       DAOSGL010000318         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_38635_length_2587_cov_3.750395, whole genome shotgun sequence.
ACCESSION   DAOSGL010000318 DAOSGL010000000
VERSION     DAOSGL010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38635_length_2587_cov_3.750395"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /gene="carB"
                     /locus_tag="PKY68_08625"
     CDS             complement(<1..1496)
                     /gene="carB"
                     /locus_tag="PKY68_08625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOH25130.1"
                     /translation="MNQDLNKVLVLGSGALKIGQAGEFDYSGSQALKALREEGIRSVL
                     VNPNIATIQTSEGIADTVYFLPVTPYFVEKIIEKERPDGILLAFGGQTALNCGTELYL
                     SGVLKQYGVKVLGTSVEAIMYTEDRDLFVKKLNEIKLKTPVSQAVETMEAALKAADKI
                     GYPVMIRSAYALGGQGSGICTNREEFITLAQNAFSFSKQILIEESLKGWKEIEFEVIR
                     DKNNHCFTVANMENVDPLGIHTGESIVVAPTCSLDDTELNMLIELSKKCVRHLGIVGE
                     CNIQYAFNSETDDYRIIEVNARLSRSSALASKATGYPLAFVAAKLALGYTLDQIGEMG
                     TSNSAYIAPQLDYIICKIPRWDLTKFAGVSREIGSSMKSVGEIMSIGKSFEETIQKGL
                     RMIGQGMHGFVGNDGAEFENLDQELSRPTDLRIFAIADALKKGYSVERICELTKIDPW
                     FVGKLKNIYDYTLVLKQYEKIEELPEDILLEAKRLGFSDFQIARFVENP"
     gene            1660..>2587
                     /gene="aspS"
                     /locus_tag="PKY68_08630"
     CDS             1660..>2587
                     /gene="aspS"
                     /locus_tag="PKY68_08630"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOH25131.1"
                     /translation="MFRTHTCGELRIHDVSKKVCLAGWVQRIRKMGAVTFVDLRDRYG
                     LTQLVVDSEAGAACTETINKMGREWVIQVHGLVRERSGKNPQLVTGDIEIQVEEIKVL
                     NAAATPPFTIEEQSDGGDELRMTYRYLDLRRPPLSRNLQLRHKMAMATRNYLDKQGFL
                     EIETPVLIKSTPEGARDFVVPSRMNPGQFYALPQSPQMFKQLLMVSGYDRYFQLVKCF
                     RDEDLRADRQPEFTQIDCEMSFIEQEDILNTFEGLIRYLFKEIKGIEFKEAFRRMSWA
                     DAMRYYGSDKPDLRFGMSFVEIKHLTKGRSFNV"
BASE COUNT          697 a          568 c          609 g          713 t
ORIGIN      
        1 ggattttcga caaaacgggc aatctgaaaa tccgagaaac cgaggcgttt agcctccagt
       61 aagatatcct cgggtaactc ctcaattttt tcgtattgtt ttaaaaccag agtgtaatcg
      121 tatatatttt tgagcttacc gacaaaccac ggatcaattt tggttaactc acagatacgt
      181 tccacgctat accctttttt cagggcatcg gctatggcaa aaatacgcaa atcggtaggg
      241 cgggacagtt cctggtcaag attctcgaat tcggcaccgt cgttgcccac aaaaccgtgc
      301 ataccttgtc cgatcattcg aagtcctttc tgaatggttt cttcaaaaga ttttcctatg
      361 gacatgattt cgcccacaga tttcatagaa gaacctattt cgcgcgaaac gccggcaaat
      421 ttggttaaat cccagcgggg tattttgcaa atgatataat cgagctgagg ggctatataa
      481 gctgaattac tggtgcccat ttctccaatc tggtccagtg tataacccaa agccagtttg
      541 gcagctacga aggcgagggg ataaccggta gctttggaag ccagtgccga agaacggctt
      601 aggcgggcat tcacttcgat aatgcggtaa tcgtcggttt ccgaattgaa ggcatactga
      661 atattgcatt cgcctacgat acccaaatgc cggacgcatt ttttggagag ttctatcagc
      721 atattgagct ctgtgtcgtc cagcgagcag gtgggagcca ccacgatgga ttctcccgta
      781 tggatgccaa gagggtcgac gttttccata ttggctacgg taaaacagtg gttgtttttg
      841 tcgcgaatga cttcgaattc gatttctttc caacctttga gcgactcttc aattaaaatc
      901 tgttttgaaa aggagaaggc gttttgggcc aatgtgatga actcttcccg attcgtacaa
      961 atgccgctgc cttgtcctcc gagggcataa gccgaacgaa tcataaccgg atagccgatt
     1021 ttgtcggctg ccttcagggc tgcctccata gtttccacag cttggctcac cggagttttc
     1081 aactttattt cgttcagttt tttaacgaac agatcgcggt cttcggtata catgatggct
     1141 tcgaccgaag tgccgagtac tttgacaccg tactgtttaa gcactcccga aaggtaaagt
     1201 tcggtaccgc aattcagggc tgtctgtcct ccaaaagcca acaagatccc gtcggggcgc
     1261 tctttttcaa tgatcttttc tacaaaataa ggagtgaccg gtagaaaata aacagtatct
     1321 gcaattcctt cagaggtttg aatggttgca atattgggat ttaccaacac cgaacggatg
     1381 ccttcttcac gcaaagcttt gagggcttgc gagcctgagt agtcgaattc gcctgcctgt
     1441 ccgattttta aagcgcctga ccccagcact agcactttat taagatcttg attcatagga
     1501 ttataaaaag atgtaagtgg tctgttcctt gtgaatgcaa aggaagcaca aattttgtac
     1561 atctcaaaag accctgcagg ttaaagaata aaatttacaa gcaagacatt aaaaatgtca
     1621 tcggaaaatg tacctttgca gtcttaaaca aagaagttta tgttcagaac acatacttgc
     1681 ggagaactcc gtattcatga tgtaagcaaa aaagtctgtt tggctggctg ggtacagcgt
     1741 atacgcaaaa tgggagctgt gacttttgtt gatcttcgcg atcgttacgg tcttactcaa
     1801 ttggtagtcg actcggaagc cggtgccgct tgcactgaaa cgataaataa aatgggccgg
     1861 gaatgggtga ttcaggtgca tggcctggtc agggaacgat ccggaaagaa ccctcaattg
     1921 gtcaccggtg atattgaaat tcaggtcgaa gaaatcaagg ttttaaacgc tgccgcaacg
     1981 ccccctttta ccatcgaaga gcaatccgac gggggagacg agcttagaat gacctaccgc
     2041 tatttggatc tgaggcgtcc gcccttgagc aggaatttgc agctgagaca taaaatggct
     2101 atggccaccc gcaactatct ggataagcag ggattcctcg aaatagagac cccggtactg
     2161 attaaatcca ctcccgaggg agccagagat ttcgtggtac cctccagaat gaatccgggt
     2221 cagttttatg ctttgcccca atcgcctcaa atgtttaaac aattgttgat ggtttcgggt
     2281 tatgaccggt actttcagtt ggttaaatgt ttcagggacg aagacctgag agccgaccgc
     2341 caacccgaat ttacccagat cgattgtgaa atgtcgttca ttgaacaaga agacatactt
     2401 aacacctttg agggtttgat tcgttatttg ttcaaagaaa tcaaaggcat tgaatttaaa
     2461 gaggctttca ggcgaatgag ctgggccgat gccatgcgtt attacggcag tgataaacct
     2521 gatttgcgct ttggtatgtc ttttgttgaa attaagcacc ttacgaaggg gcgttctttc
     2581 aatgttt
//
LOCUS       DAOSGL010000319         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_39529_length_2547_cov_4.060193, whole genome shotgun sequence.
ACCESSION   DAOSGL010000319 DAOSGL010000000
VERSION     DAOSGL010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39529_length_2547_cov_4.060193"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKY68_08635"
     CDS             <1..653
                     /locus_tag="PKY68_08635"
                     /inference="COORDINATES: protein motif:HMM:NF023746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOH25132.1"
                     /translation="LKADDTLTIAAKSIAEIITVPGYINLDFADVNTILKDGGVAVMS
                     AGYGKGQNRLTEALNSALKSPLLNNNDVFRARKILFNITCSDKSEIMTHEMEQIRIFM
                     DQFVNQVEEVIWGIAADNDLDDQIKVTMLASGFGIDDVPQVKVHRQIKSRELEEDIEE
                     KRRKRENRKSTYYPDDFGIPPRRLIYKPRVLSNQEMDNEELIDQLDQQPANQRPFA"
     gene            complement(670..1440)
                     /gene="argB"
                     /locus_tag="PKY68_08640"
     CDS             complement(670..1440)
                     /gene="argB"
                     /locus_tag="PKY68_08640"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOH25133.1"
                     /translation="MDKLNIIKVGGKVVEDPATLEQFLQDFAKISGYKLLVHGGGTLA
                     TELAKQLGVENKITEGRRITDAETLKIVTMVYGGLINKNIVACLQAKNINALGLTGAD
                     LNLIRSDKRPVKEIDYGYVGDVKEVQDDLLAELLKLGIVPVLAPITHDKNGQLLNTNA
                     DTIAAETAKALAEYFDVHLIYCFEKKGVLMDPEDDNSVIPEIDASLFKEYVEQGIIRE
                     GMIPKLQNAFEAIHCGVKDVTITKAQDILLGKGTRIML"
     gene            complement(1455..>2547)
                     /locus_tag="PKY68_08645"
     CDS             complement(1455..>2547)
                     /locus_tag="PKY68_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOH25134.1"
                     /translation="TYGGNFSESVVPTAYSSGNSFYVKVNSPSANYWDRELKWRVPVR
                     RGMRYKISFLAKSNSQEPFHIEMQRLNGSAYNLRTTFVCNTAMRTSTFITGDAGESSG
                     IYVLNFQYGTHPAGTELWIDEVKLEELSGKDYGNYVTNGDFETGTEGWNMNYSGNVAV
                     SLDRHNPLNGQASLYGKVGNASSLIRSMAGWKVYLFKGRQYRLEFDARGSEGSVLSVF
                     FDTQTAKRIENTLHPETRHYSLLLDSLSESKEYTLNFSFSKNEQNSECWIDNIELYDY
                     KEPAAIVNPGGQSENHALLYPNPGKGLFYLDEAAAPAGSRFYIYNSAGILIKELGLRG
                     RELDLTELQAGLYYVKWQGRQQLQKIIIQ"
BASE COUNT          600 a          613 c          616 g          718 t
ORIGIN      
        1 ttctcaaggc agacgatact ttgacgatag ccgccaaaag cattgccgaa atcattacgg
       61 tgccgggcta tatcaatctc gattttgcag acgtgaatac gattctgaaa gacggtggag
      121 tggcagtgat gagtgccggt tacggaaagg gacaaaaccg tctgaccgag gctttgaatt
      181 cggccttgaa atctccttta ctcaataaca acgatgtatt tcgtgcccgc aagatattgt
      241 tcaacatcac ctgcagcgat aagtcggaga tcatgactca tgaaatggag caaatcagga
      301 ttttcatgga tcagtttgtc aaccaggtgg aagaagtcat ttggggaatt gcagccgaca
      361 acgatctgga cgatcaaata aaagtgacca tgctggccag tggctttggt atagacgacg
      421 tacctcaggt taaggtgcac aggcaaatca aaagccggga actggaagaa gacatcgaag
      481 aaaagcggcg taaacgtgaa aaccgcaaaa gcacttatta tcccgacgat tttggcattc
      541 ctccccgcag gcttatttac aagcccaggg tgttgagcaa tcaggaaatg gataatgaag
      601 aactgataga tcagctcgat caacaaccgg ccaatcagcg tccttttgcc tgatcaccat
      661 tagactagac tacagcataa tccgggtccc tttgcccagt aggatgtcct gagccttggt
      721 aatggtgacg tcctttactc cgcaatggat ggcttcgaaa gcgttttgca gtttggggat
      781 cataccttcc cggataatgc cctgctcgac gtattctttg aacaagctgg catcgatttc
      841 ggggatcacg ctgttatcgt cctcgggatc catcaggact cctttctttt cgaagcaata
      901 aataaggtga acatcgaagt attccgccag tgctttagcc gtctcggcgg caatggtgtc
      961 ggcattggta ttgagcaatt gcccattttt atcgtgggta atgggagcca gcaccggcac
     1021 aatgcccagt ttcaacaatt cggctaagag atcgtcctgt acctccttta catcgcccac
     1081 gtagccgtag tcgatttctt ttacgggcct tttgtccgat cttatcaggt ttaggtcggc
     1141 tccggtcagt cccagggcgt ttatgttttt ggcttgcaaa caggcaacaa tgtttttgtt
     1201 gatcagaccc ccgtacacca tggtgacaat cttgagtgtt tcggcatcgg taatacgccg
     1261 gccctcggtt attttgtttt ctacacccag ctgcttagcc agttccgtag ccagggtgcc
     1321 tccaccgtga accagtagtt tatatccgga tattttggca aagtcttgca gaaattgttc
     1381 cagggttgct gggtcttcca ctactttacc ccccactttt atgatgttta atttatccat
     1441 gttttcaagc tgatttattg aataataatt ttttgcaact gctgacggcc ctgccattta
     1501 acgtagtata aaccggcctg caactctgtc aggtcaagct ctctgcctct gagaccgagt
     1561 tcttttatca ggattcccgc actgttgtat atataaaagc gactgcctgc aggcgctgcc
     1621 gcttcgtcca ggtaaaacag gcccttcccc ggattaggat acaacagggc atggttttca
     1681 ctttgtccac cggggtttac gattgctgcc ggttctttgt aatcataaag ttcgatattg
     1741 tctatccaac attcgctgtt ttgctcgttt ttactgaacg aaaagttaag ggtgtattct
     1801 ttagattcgc tcaggctgtc caacagcagg ctgtaatgcc gggtttcggg atggagtgta
     1861 ttttctatcc ttttggcggt ttgcgtgtcg aagaaaacgc tgagcacact gccttcactg
     1921 ccgcgggcat cgaattccag ccggtattgc ctgcctttga ataaataaac tttccaaccg
     1981 gccatacttc ttatcagact ggatgcgttg ccaactttgc cgtatagcga agcttggccg
     2041 ttcaggggat tgtgccggtc aagactaaca gccacgttcc ccgaataatt catgttccat
     2101 ccctctgtgc cggtttcgaa atcgccgttg gtgacgtagt tgccgtagtc tttgcccgag
     2161 agttcttcca atttaacttc gtctatccac aactcagtgc ctgcggggtg agtaccgtac
     2221 tgaaagttca gcacgtagat tccgctcgat tctccggcgt ccccggtaat gaaagtcgaa
     2281 gtccgcatgg ccgtgttgca gacgaaagta gttctcaggt tgtaagcgct gccgttgagg
     2341 cgttgcattt cgatatgaaa gggttcctgg gaattggatt tggccagaaa gctgattttg
     2401 tagcgcatac cccttcttac cgggactctc catttgagtt ccctgtccca ataattggcg
     2461 gaagggctgt tgactttaac ataaaacgaa tttccgctgc tgtaggctgt gggcactacg
     2521 ctttcgctga aattgccgcc ataggtg
//
LOCUS       DAOSGL010000320         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_40036_length_2525_cov_3.751012, whole genome shotgun sequence.
ACCESSION   DAOSGL010000320 DAOSGL010000000
VERSION     DAOSGL010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40036_length_2525_cov_3.751012"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKY68_08650"
     CDS             <1..785
                     /locus_tag="PKY68_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25135.1"
                     /translation="KTILVSDYIGFYEQDPVLGINTLRDHLAGRMKSLIIDIRNTEYY
                     ETIYGLSRLLSPAYIRALALKNSAIDHFKACRRLSGRLEKFFENPEKQKQSAILKEFY
                     NIYELLPGTQEEKYCLSDDLRKKLNRKTAHYLFAALALTPAYLINWPVRKIIQAIIKL
                     DKDPQMHSSYRFVLYALMLPLWYITLSLLLALSFKWCLIKTSVFFLVLLLVACFGERF
                     IPAFSLESRLWRLLQKQPSDLRSRLISTLDKLEDLCRDFFVA"
     gene            828..2525
                     /locus_tag="PKY68_08655"
                     /pseudo
     CDS             828..2525
                     /locus_tag="PKY68_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036958.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
BASE COUNT          691 a          573 c          566 g          695 t
ORIGIN      
        1 gcaaaactat tttagtatcg gattacatag gattttacga acaagatccg gttcttggca
       61 taaatacttt gcgagaccat ttagccggca ggatgaaaag cctgataatc gatattcgaa
      121 atacagaata ttatgaaaca atatatggct tgtcccgcct gttaagtccg gcttatattc
      181 gggccctggc tttaaaaaac tcggccattg accattttaa agcctgtcgt cgcttaagcg
      241 gcaggctcga aaaatttttc gaaaatccgg aaaaacaaaa acagtcagct atccttaaag
      301 aattttacaa catttacgag cttcttcccg gtactcagga agagaaatac tgtttgtcgg
      361 acgacctaag aaaaaaactg aataggaaaa ctgcacatta tcttttcgcc gcgttggccc
      421 ttacgccggc ttatcttata aactggccgg tacgaaaaat catacaggca ataataaagc
      481 ttgacaaaga tcctcaaatg cacagcagtt accgttttgt cctctacgca ctgatgttgc
      541 cgctttggta tataacactg agcctcctgt tggcgctttc gtttaaatgg tgtctgataa
      601 aaacaagcgt ctttttcctc gtacttttgc ttgtagcttg tttcggggaa cgtttcatac
      661 cggcattcag cttggaatcc aggctttgga ggctcttgca aaagcagccg tcagacttgc
      721 gttcaaggct gatttcaacc ctggataaat tagaagactt atgccgggat ttttttgtcg
      781 catagggcaa tttctgctaa ctttgctggc tcttattgct gaattttgtg gagaatattt
      841 taaataaact caacgacagt caaaagcaag ctgtactgta taacgacgga ccttcgttgg
      901 tcatagcggg ggccggttcg ggcaagaccc gggtgttgac ctgcaaggta gccgttttgc
      961 ttaagcaggg tctggccccc tggaatatca tggctcttac ctttaccaac aaagccgcca
     1021 gggagatgaa ggaacgcatt gccggcctgg tcgatccggc atcggcttct ctgctgtgga
     1081 tgggtacttt tcactctgtt ttcctgcgga ttttgcgagc cgaagcttct gtcttgggct
     1141 atccttcgtc ttttagcatt tacgacacta ccgatagcaa gaatatgatc aaaagcatcg
     1201 ttcgtgattt aggtttggac gaaaaaattt atcgtgaagg ggctgtgcat gcccgtattt
     1261 catcggccaa aaacgatttg atctcgcctg ccgcctatgc gaataatccg gccctgctcg
     1321 agagggattt attcaccaaa atgccccgga tagttgacat ttaccagcgc tacgttcaac
     1381 gttgccggct ggcttcggct atggactttg acgatctcct gtaacacaaa tgttttattt
     1441 cgcgatcatc ccgaaatact caaagcctat caatctcgct ttcagtttat tttggtcgat
     1501 gaataccaag ataccaattt ctcacaacac cttatagtca gtcgtttagc tgaacggcac
     1561 cacagggtct gtgtggtggg tgacgattcg cagagcattt attcgttcag aggagccaat
     1621 atcgacaaca ttctgaattt tgaaaagacc tatcccgagt gcttgttgtt taaattggaa
     1681 cagaattacc gctccactaa aaatattgtc aacttagcca acagcctgat tgccaaaaac
     1741 aagatacaaa ttcccaaatc ggtattttcc aaacgggaaa ccgggaacaa aatatcggtt
     1801 ctgagcgctt tttcggatat ggaagaagcc tatatgatcg caaacaagat caatgagatg
     1861 catatgagtt tgtacgatcc ctggagcgaa tttgccgtac tttatcgcac caatgctcag
     1921 tcccggatac tggaagatgc tatgcgcaag agaggcatcc cctatcgcat ttatggcggt
     1981 atttcttttt accaacgcaa ggaaatcaag gatattattg cttacctgag gttggttatc
     2041 aatcctgccg acgaagaggc ctttaaacgc gttgtgaatt tcccggccag gggcataggc
     2101 aaaaccactc tggacaaaat tcagcaggcg gccgccctgc accggaccac tttttgggaa
     2161 atctgctcca agcccctctc gtacaacttg cctgtacata gcggcaccga aaataagttg
     2221 aaacagttta gttcgcttat cgagaacctg atgcctttag cttcgacact gaatgtgtat
     2281 gagtttacca aaaaaatgct cgaagaaagc ggcatcaaca atgccctctt gcaagacagt
     2341 tccgaggaag gaatcagccg gcaggaacat ttggaatcgc tgctttcggg catgtttgat
     2401 ttttgccaaa gtcgtctgga agagggaaat gaacaaatgc tgctcccgga ctacctggcc
     2461 gaaatctccc ttttgaccga tcaggacgaa gttgatgagg gtgaaaaggt aaatttaatg
     2521 accgt
//
LOCUS       DAOSGL010000321         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_40501_length_2506_cov_3.665035, whole genome shotgun sequence.
ACCESSION   DAOSGL010000321 DAOSGL010000000
VERSION     DAOSGL010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40501_length_2506_cov_3.665035"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="PKY68_08660"
     CDS             complement(<1..1677)
                     /locus_tag="PKY68_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25136.1"
                     /translation="MKTPYYLLSFALLLVFSACQPKVQLLTTGSDSPRGNYAKERLSN
                     SLVSNGYTIVDENVAGKVTQLYLGCQNDLNFQQMLQEQGIAIEPVEGKEAFRIVADGD
                     KIAVCGADESGLLYGCMELIDRLKTNGRLPKKIDYSDAPDMVLRGTCIGMQKMYLLPG
                     RKVYEYPYTQELFPWFYDKELWIKYLDMLVENRMNSVYLWNGHPFASLVKLPDYPYAV
                     EVSEEDFERNEEMFGFLAQEANKRGIWVIQMFYNIIVSQPFADYHKIPTQDRKVEITP
                     LLSDYTRKSVAAFIEKYPNVGLLVCLGEAMNTHEDDVEWFTKTIIPGVQDGLKALGLE
                     QEPPIVLRAHDTNAEMVMDAALPIYKNLYTMNKYNGESLCTYQPRGPWTATHQALSAL
                     GSVHISNVHILANLEPFRYGSPDFIQKSIQAMHNIHGANGLHLYPQASYWDWPYAADK
                     IEDGSRLLEMDRDWIWYQSWARYAWSADRDRKEEIDYWSGLLSELYDCGKQGKYILEA
                     YEQSGEISPKLLRKFGITEGNRQTFLLGMFMSQLVNPNRWTVYPDFANSCG"
     gene            complement(1888..>2506)
                     /locus_tag="PKY68_08665"
     CDS             complement(1888..>2506)
                     /locus_tag="PKY68_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOH25137.1"
                     /translation="CYLDDYTIMRPLMMDFANDNRVMNIGDQYMFGSHLMVCPVYEYK
                     ARRRLVYFPQNAGWYDLYSGKYYPGGQESPVEAPYERMPVFVPAGSILPTGKPIQHSG
                     EKQETLRFYVFSGQDGKACLYEDDATSYDYEKGDYARITFHYNDAQKSLSISAIEGSY
                     RPEPAQRRFEFVLVSPQSPAGLDTKVPKTAGLVYSGQETKLDFDN"
BASE COUNT          639 a          570 c          605 g          692 t
ORIGIN      
        1 gccgcaggaa ttggcaaaat cgggatagac cgtccagcgg ttggggttga ccaactgact
       61 cataaacatt cccagcagga aggtttgccg attgccctcg gtgataccga atttgcgcag
      121 caatttaggc gatatttctc cactttgttc gtaggcctcc aaaatgtact ttccttgttt
      181 gccgcagtca taaagttcgg ataataagcc cgaccaataa tcgatttctt ccttacgatc
      241 gcgatcggcc gaccaggcat aacgcgccca ggattgatac cagatccagt cgcggtccat
      301 ttcgagcaga cggctgccgt cttcgatttt gtctgctgca taaggccagt cccagtatga
      361 ggcctgggga tagaggtgca aaccgttggc tccgtgtatg ttgtgcatag cttgaatgct
      421 cttttgtata aagtcgggcg agccgtagcg aaaaggttcc aggttggcca aaatatggac
      481 attggagatg tgaacagatc ccagagcgct gagtgcctga tgtgtagccg tccaggggcc
      541 tctgggttga taggtgcaca gggattcgcc gttgtatttg ttcatggtgt agagattctt
      601 gtaaataggc aaggccgcat ccatgaccat ttcggcgttg gtatcgtgtg ccctgagcac
      661 aataggcggt tcctgctcta atccgagagc tttgagtccg tcttgcacgc cgggaataat
      721 ggttttagta aaccattcca catcgtcttc gtgagtgttc atggcttcgc ccagacagac
      781 cagtaaacct acgttggggt atttttcgat aaaagccgcg acagatttac gggtgtaatc
      841 cgacagcaaa ggagtaattt cgactttgcg gtcttgagtg ggtattttgt gataatctgc
      901 aaagggttga gatacaataa tattgtaaaa catctgaatc acccaaatgc cccgtttgtt
      961 ggcctcctga gcaagaaagc cgaacatttc ttcgtttcgc tcgaaatctt cttcgctcac
     1021 ttccacagca tagggataat cgggtagttt aaccagcgaa gcaaagggat gtccgttcca
     1081 gagataaacg gaattcatgc gattttccac cagcatatcc agatacttga tccagagttc
     1141 tttatcgtaa aaccagggga agagctcctg ggtataggga tattcgtaaa ccttacgacc
     1201 cggtagcaaa tacatttttt gcatgccgat gcaagttccc cttaacacca tatcgggagc
     1261 atcggaataa tctattttct taggtagccg tccgttcgtt ttcaaacgat cgatcagttc
     1321 catgcatccg taaagcaagc cgctttcgtc ggctccgcag accgctattt tatctccgtc
     1381 tgcaacaatc ctgaatgctt ctttgccttc gacgggttct atggcaattc cttgttcttg
     1441 taacatctgc tgaaaattca gatcgttttg gcagccgaga tatagttggg ttaccttgcc
     1501 ggcaacgttt tcatcaacaa tagtatagcc attggaaacc aagctgtttg ataatctttc
     1561 ttttgcgtaa ttacctctag gagaatccga ccctgtcgtc agcagttgaa ctttgggttg
     1621 acaggcagaa aacactaaga gcaaagcaaa actcaggaga taatagggag ttttcatgaa
     1681 gaataggttt aaacgtttca gaattacaaa agtaggatta tttcagtatt gtaaaattaa
     1741 aaaaatcaaa aacttgcgct gtgggggctg tcctaacaaa aaaagaaaat ttggtgtggc
     1801 tccaatctgg ataccggtta ctcttaatat tggtgaaaat ccggacgatg gtaaaagtcc
     1861 aatgacagaa tcttttttac aggtctatca attgtcgaaa tcaagctttg tctcttgccc
     1921 cgaatacacc aggccggctg tttttgggac tttggtgtcc aggccggcag gagattgggg
     1981 actgaccaaa acgaattcaa accgcctttg agccggctcg ggacgatagg aaccctcgat
     2041 agctgaaatc gacaaacttt tttgggcatc gttgtaatgg aaggttatcc tggcatagtc
     2101 acccttttcg taatcgtagc tggttgcgtc gtcttcgtac aagcaggcct tgccgtcttg
     2161 tccgctgaat acataaaaac gcagtgtttc ttgtttttcc ccactgtgtt gaatgggctt
     2221 acccgtggga agtatgcttc ctgcaggcac aaaaaccggc atgcgctcgt aaggggcttc
     2281 caccggactt tcctgtcctc ccggataata cttgccgctg taaagatcgt accagccggc
     2341 gttttggggg aaataaacca gtcgcctgcg ggctttatat tcgtacaccg ggcagaccat
     2401 caagtgagat ccgaacatat attggtcacc gatattcatt acccggttgt cgttggcgaa
     2461 atccatcatc aagggacgca tgatggtgta atcgtctaag taacat
//
LOCUS       DAOSGL010000322         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_41120_length_2480_cov_4.496082, whole genome shotgun sequence.
ACCESSION   DAOSGL010000322 DAOSGL010000000
VERSION     DAOSGL010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41120_length_2480_cov_4.496082"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..820
                     /locus_tag="PKY68_08670"
     CDS             155..820
                     /locus_tag="PKY68_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOH25138.1"
                     /translation="MKNNLYLLLILLLSACQSKQGSGSIDWKVAALPDVEYQLGEPRL
                     LDTELGPVVQFFGNEAYFLNSNPLAGMEEVTVEAIFRPDADGSFEQRFLHLGEISGER
                     IMLETRVKPDGNWYFDAYVKRPDGEGCALIDTNLIHPTEQWYHVAFVCRKDSLFSYVN
                     GQFEKSAAFAYQTINKGISSVGVRQNKVCFFKGQMYRIRITPGALTPEEFLQDHLILN
                     SYE"
     gene            813..1148
                     /locus_tag="PKY68_08675"
     CDS             813..1148
                     /locus_tag="PKY68_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH25139.1"
                     /translation="MNSPYFLMEKELPWTDLGGGVKRQILGYNQDIMMVKVAFEKGSI
                     GAAHKHPHTQSSLCVSGKFEVDIEGQRSVVSAGDGFYVAPDLVHGVRCLEAGMLLDAF
                     APCRKDFLP"
     gene            1176..2018
                     /locus_tag="PKY68_08680"
     CDS             1176..2018
                     /locus_tag="PKY68_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOH25140.1"
                     /translation="MKQIAIQGISGSYHDIVAHHYFEGEEIEILGCKTFKDVFATMRK
                     DSSILGVIAIENTIAGSLLQNHNLLRESETAIIGEFKLRISHCLAAILGQSFKDIREV
                     HSHPIALMQCDDYLGLHPEWTIVESEDTATSAKTIGNQKIMGRAAICSRQAAEIYGLE
                     VLEAGIETNKHNFTRFLVLADRWDAEKFNANNKINKSSMVFSTYHREGALSQVLSVLS
                     YYHNNLTKIQSIPIIGHEWEYLFFVDLTFEDRLKYKQSLEAIRPLTSNLKILGEYPVG
                     RQML"
     gene            2122..>2480
                     /locus_tag="PKY68_08685"
     CDS             2122..>2480
                     /locus_tag="PKY68_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25141.1"
                     /translation="MKIIEVNTPALRKEFALLPFRLYRGNPYWVPPILKSEINNINPA
                     VNPFFRFCDAKFWIVQKNGLTVGRIGAIIHYAYIEKTQEKYGRFTRAEFIDDKAVVDV
                     LFETAENWIKSKGMNGA"
BASE COUNT          715 a          567 c          570 g          628 t
ORIGIN      
        1 cctgtgcccg agctgtacga agaaaccatg aaacatccct tcaacacgca gttttatcgt
       61 tccgaaaatc cacctttggg catctcggtc aatgcctatg tgcaacatat gggagttttg
      121 attcaatact tgctgagcca aaaagagaac tcaaatgaaa aataatcttt atctcctgct
      181 catactcctg ctgagtgcct gtcaatccaa acagggctcc ggcagtatcg attggaaggt
      241 agcagcactt ccggatgtgg aatatcaact gggagaaccg cggttgctcg atactgaatt
      301 agggccggtc gttcagtttt ttggaaacga ggcttacttt ttgaacagca atcctctggc
      361 gggcatggaa gaagtaacgg tcgaagctat tttccgtccg gatgccgacg gatcgtttga
      421 acaacgcttt ttgcacctgg gcgaaatctc cggcgaacgc attatgctcg agaccagggt
      481 aaaacccgat ggaaactggt atttcgatgc ctatgtaaaa cgtcccgacg gagaaggctg
      541 tgccttaatc gataccaacc ttattcaccc gacagaacag tggtatcatg ttgcttttgt
      601 ctgtcgcaaa gacagcctgt tttcctatgt aaacggtcaa tttgaaaagt cggcggcttt
      661 cgcctatcaa accataaaca agggaattag ctctgtggga gtgcgtcaaa acaaggtttg
      721 ttttttcaag ggacaaatgt accgcatccg cattacaccc ggagccctta cacccgagga
      781 atttttacaa gatcatttaa tcctgaatag ttatgaatag tccttacttt ttgatggaaa
      841 aagagctacc ctggaccgat ttgggcgggg gagttaaaag acaaatcctt ggttacaacc
      901 aagacattat gatggtaaaa gttgccttcg aaaaaggcag catcggagcg gcacacaagc
      961 atccgcatac ccagtcgtcg ctctgcgtca gcggtaaatt cgaagtggat atcgaggggc
     1021 aacgctccgt tgtgtctgcc ggagacggtt tttacgttgc gcccgacctg gtgcacggag
     1081 tccgctgtct cgaagccggc atgctcctgg atgcttttgc tccctgccgc aaagattttt
     1141 tgccttaatg ccgggatttg cacacaaaaa atgatatgaa gcaaatagcc atacaaggca
     1201 tttcgggctc ttaccacgat atagtggcac accattactt cgagggcgaa gaaatcgaaa
     1261 tactgggatg caaaacattc aaagatgtgt ttgctaccat gcgaaaagac agcagtatat
     1321 tgggtgtgat tgccatcgaa aataccatag ccggcagcct gctccagaac cacaacctgc
     1381 taagagagag cgagaccgcc attataggag aattcaagct ccggatcagc cattgcctgg
     1441 ccgctatcct cgggcaaagc ttcaaagata tacgtgaagt gcattctcac cccatagctc
     1501 tgatgcaatg cgacgattat ctggggttac atcccgaatg gactatagtg gaaagcgaag
     1561 ataccgccac cagtgctaaa accataggca atcaaaaaat tatgggcaga gctgccattt
     1621 gcagccgcca ggccgccgaa atttacggac ttgaagtgct ggaagcaggc atagagacca
     1681 acaaacacaa ctttacccgt tttttggttt tggccgatcg ttgggatgcc gaaaaattca
     1741 acgccaacaa caagataaat aaatcttcga tggttttctc cacttaccac cgggagggag
     1801 ccttatctca ggttttgtcg gtgttgtcgt attaccacaa caacctgacc aaaattcaat
     1861 cgattcctat cattggccat gaatgggaat atttgttttt tgtcgacctt actttcgaag
     1921 atcgcctcaa atacaagcaa agtctcgaag cgatacgccc cttgacaagc aacctgaaaa
     1981 tcctgggtga atacccggtg ggcaggcaaa tgctttgaat aaaaatactg tacttattta
     2041 aattcacgtt taaaagcggt aagtttaagc ccgattatag tatatttgtt gattattaag
     2101 aggaaattaa aagagttaaa aatgaaaatt atagaagtaa acactccggc tttgcgtaaa
     2161 gaatttgcgc tcctgccttt tcgtttgtac cggggaaacc cttattgggt gcctcctatc
     2221 ttaaagtcag aaatcaacaa catcaacccg gccgtcaatc cgtttttccg tttttgcgat
     2281 gccaaatttt ggattgttca aaaaaacggc cttacggtcg ggcgcattgg tgccatcatc
     2341 cattatgcct acatcgaaaa aacccaggaa aaatacggac gtttcaccag agcggaattc
     2401 atcgacgaca aggcagtggt tgacgttttg tttgagacag cggaaaattg gataaaatcc
     2461 aagggtatga acggagcaat
//
LOCUS       DAOSGL010000323         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_41769_length_2454_cov_3.096707, whole genome shotgun sequence.
ACCESSION   DAOSGL010000323 DAOSGL010000000
VERSION     DAOSGL010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41769_length_2454_cov_3.096707"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PKY68_08690"
     CDS             <1..1512
                     /locus_tag="PKY68_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOH25142.1"
                     /translation="LLGSDVVSRMLIWGELRSDNMVQGQGIPDAENEILRANILPSNP
                     YTGWTCFYNVINRCNTVLYYAPMVAQKDPNFNESSLKAIEAEAKALRALCYFYLMRTF
                     RDVPYASEPSVDDNQRYDLAPTPFDELLQILIQDLEGCKDYAIKAYGNKAYNTGRITR
                     DAIHALLADLYLWDQDYDNCIRYCDLVIQSKREQYNELNKDKEELASNEYPLIEAETP
                     GSSVTGNSYNQVFGTGNSTESIFELYFLESPGRLTNTTVSRYYGSVNAQRGYLGAPDF
                     IGRDRNGGSNVFLYGNEVFAKKDVRSREYIGRSSGGVLPITKYAYRSVSNENGVYRST
                     DFANWIIYRLSDVMLMKAEALTNMISETAEEDTASSEAAQAKILLSQSFELVRAVYNR
                     SNPEAGRDSLNYTNYSSKDNMNALILAERQRELMFEGKRWFDLVRMARREGNNAKLVD
                     MVISKYTENQSTIRTKMMSVDALYFPYNEEELKVNSYLKQNPVYITETSSITR"
     gene            1524..>2454
                     /locus_tag="PKY68_08695"
     CDS             1524..>2454
                     /locus_tag="PKY68_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH25143.1"
                     /translation="MKIPIFSPLRFVYGLIALLVLASCNYEPDKENFYTFTGEMVADY
                     LENRPERFSEFIKILERADKMDLLDTYGSFTCFAPTNRAVEEYLALYNLSSVEELSDK
                     QCDTIANSHLINRAYFTTDMTDGVISTPNQFDRYLSISFVVADSLSGTMAYFINKQSQ
                     IVLRDDSVENGVVHTIDKVIRPSNSLLPELMREDTTIRLFYEAMVLTTFDDSLLLYMD
                     YDYVQPKLVLHYTSHVNRETAIAPKYRKYGYTGFVEPDEVYRQKGINNMNDLIAYAKK
                     VYDEVYPQDANLYDDDFTHPKNPLNRFVAYHFMP"
BASE COUNT          671 a          575 c          559 g          649 t
ORIGIN      
        1 ctgttgggta gtgatgtcgt aagccgtatg ttgatttggg gagaactcag atcggacaat
       61 atggtgcagg gtcagggtat ccccgatgcc gaaaacgaga tactcagggc caatatattg
      121 ccaagcaatc cttataccgg ttggacctgt ttttacaatg tgatcaaccg ttgcaatacc
      181 gtgttgtatt atgcgccgat ggtagcccaa aaggatccca atttcaatga gtccagcctc
      241 aaagccatag aggcggaggc taaagccctt cgcgccctct gttatttcta tctgatgcgt
      301 actttccgtg atgtgcccta tgcctcggag ccttctgtgg acgacaatca gcgctacgat
      361 ctggcgccga ctcctttcga tgagttgttg cagattttga ttcaggatct ggaagggtgt
      421 aaagattatg ccatcaaggc ctacggtaac aaagcctaca ataccggccg cattacccgt
      481 gatgccattc atgctttgct ggccgattta tatttatggg accaggatta cgacaattgc
      541 atcaggtatt gcgatttggt catacaatcc aaacgggagc aatacaatga actcaataag
      601 gataaagaag aactggccag caacgaatac cctctgatcg aagcggaaac tcccggttcc
      661 agtgttacgg gtaactctta taatcaagtg tttggaaccg gaaattctac cgaaagtatt
      721 tttgaactct atttcctgga atctcccggc cgtttgacaa atactacggt gtcaagatat
      781 tatggcagtg ttaatgcgca gagaggttat ttgggagccc ccgactttat aggcagagac
      841 cgtaacggcg gcagtaatgt ttttttgtac ggaaacgagg tttttgccaa aaaagacgtg
      901 cgttcccgcg aatacatcgg ccgctcatcg ggtggtgtgt tacccatcac caaatacgcc
      961 taccggagtg tgtccaacga aaacggagta tatcggtcta ccgattttgc caattggatt
     1021 atttatcgcc tcagcgatgt aatgctgatg aaagccgagg ctcttacaaa tatgatttcc
     1081 gaaacggccg aggaagacac ggcatcaagc gaagctgccc aggctaagat tttgttgagc
     1141 caatcgtttg aactggttcg tgccgtttac aaccgctcca atcccgaggc gggcagagac
     1201 agcctgaatt acaccaacta cagcagtaag gacaatatga atgccctgat cctggccgaa
     1261 cgtcagcgtg aactgatgtt cgaaggaaag cgttggttcg atttggtccg catggctcgc
     1321 agggaaggta acaatgctaa gctggtcgat atggtgattt caaaatacac cgagaatcaa
     1381 agcaccatcc gtaccaaaat gatgtcggta gatgctttgt atttccctta caacgaagaa
     1441 gaattgaaag tcaattctta tttgaagcaa aatccggttt atattaccga aacatcctca
     1501 atcacccgat aaattctata caaatgaaga taccgatatt cagtccactc cgttttgttt
     1561 atggcctaat tgccttgctg gtgctggctt cctgcaatta cgaacccgac aaggagaact
     1621 tttatacttt tacaggagag atggtggccg attacctcga aaaccgtcct gagcgattca
     1681 gcgaatttat caaaatactc gaaagagccg acaagatgga tttgctggac acttacggtt
     1741 cctttacctg ttttgctccg accaaccggg ccgttgaaga atacctggcg ctctacaacc
     1801 tgagttccgt agaagaactc tccgataagc aatgcgatac cattgccaat tctcacctga
     1861 taaacagggc ttattttact accgatatga ccgacggggt gatttcgact cccaaccaat
     1921 tcgatcgcta tttatccata tcttttgtag tggccgactc cctcagcggt acgatggctt
     1981 attttatcaa caaacaatcg cagatagttc tcagggacga ctccgtcgaa aatggggtgg
     2041 tgcacaccat cgacaaagtc atcaggcctt ccaattctct tttacctgaa ttgatgagag
     2101 aagataccac tatcaggctc ttttacgagg ccatggttct gactactttt gacgactctc
     2161 ttcttttgta tatggattac gactacgtgc agccgaaatt ggttctgcat tacacttcgc
     2221 acgtcaaccg tgaaacggcc attgctccca aataccggaa atacggttat acgggttttg
     2281 tggagccgga tgaggtgtat cgtcaaaaag ggatcaacaa catgaacgat ctcatagctt
     2341 atgcaaaaaa ggtatacgac gaagtctatc cgcaggacgc caacttatac gacgatgatt
     2401 ttactcaccc taaaaatcct ctgaaccgct ttgtggctta tcactttatg cccc
//
LOCUS       DAOSGL010000324         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_41964_length_2446_cov_3.020075, whole genome shotgun sequence.
ACCESSION   DAOSGL010000324 DAOSGL010000000
VERSION     DAOSGL010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41964_length_2446_cov_3.020075"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKY68_08700"
     CDS             complement(<1..189)
                     /locus_tag="PKY68_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH25144.1"
                     /translation="MKFFEFDTIREIFSTMRQSKLRTFLTGFSVAWGIFMLIVLLGSG
                     KGLQNGVSSNFSNYAVNMV"
     gene            complement(199..897)
                     /locus_tag="PKY68_08705"
     CDS             complement(199..897)
                     /locus_tag="PKY68_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH25145.1"
                     /translation="MILLEKLNKTYPGVQPLHVLKDIDLSISQGEYVAIMGASGSGKS
                     TLLNVLGILDDYDTGRYLLDGKLIAGLSEKKAAIYRNKMIGFVFQSFNLISFKNAMEN
                     VALPLFYKGVARKKRNQLALEYLEKVGLRDWWHHYPNELSGGQKQRIAIARALISQPR
                     IILADEPTGALDSRTTDEVIQLLSQVNAEGITTIVVTHERSVAEQTKRIIHLKDGYIE
                     SDNINIPSLTACSK"
     gene            complement(977..1441)
                     /locus_tag="PKY68_08710"
     CDS             complement(977..1441)
                     /locus_tag="PKY68_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25146.1"
                     /translation="MKKTILALAVCLLGFTATFAQSKALGLRGGYGFEISYQQALGDA
                     NRLEADLGLGVFNGLNLAAVYHWVWSLESIGLPEGFKWYAGPGADLYFGFGNNEGNNN
                     YEGISAGIGGQIGVEYTFPGIPLQLAIDCRPMWLFGNFGPYWGYAGAVRYVF"
     gene            1778..>2446
                     /locus_tag="PKY68_08715"
     CDS             1778..>2446
                     /locus_tag="PKY68_08715"
                     /inference="COORDINATES: protein motif:HMM:NF017689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HOH25147.1"
                     /translation="MTDVIKIKKGLDISLKGGAFPEVQSASYTENYALCPTDFHGLIP
                     RVLVKEGEQVLAGTPLVADKNKPEILFVSPLSGVVSAVKRGEKRRLLAIEIRADQKNA
                     ALSYPVRNPLDMDGVKVKEALLEAGLWPYIKRRPYDVIANPRVEPKAIFISAFDTAPL
                     APDYAFILKGQGHDFQTGLNALTKLTKGKVHLSIPAGSGSGELKNAQNVEIHRFEGPH
                     PAGNV"
BASE COUNT          688 a          542 c          542 g          674 t
ORIGIN      
        1 caccatgttg actgcgtagt tgctaaagtt ggaacttacg ccgttctgca aacctttgcc
       61 cgagccaagc agcacgatga gcataaaaat accccaggct accgagaaac ctgtcaaaaa
      121 ggttctcagt ttgctctgcc gcatggtgct gaaaatttct ctaatggtgt cgaactcgaa
      181 aaatttcata aaacaggctt atttgctgca ggctgtcagg ctgggaatat tgatattatc
      241 ggattcaatg taaccgtctt tcagatgaat gatgcgtttg gtctgttctg caaccgacct
      301 ttcgtgggtt accacaatcg tggtgattcc ctcggcattt acctgcgaga ggagttgtat
      361 aacctcgtcg gtagtgcggc tgtccaaggc gccggtaggt tcatcggcca atataatacg
      421 gggctgggaa atcaaagccc gggcaatggc aatgcgttgt ttctgtccgc ccgacaattc
      481 gttggggtag tgatgccacc aatctctcag gcctactttt tcgaggtatt ccaaagccag
      541 ttggttgcgt ttcttgcggg cgacaccttt ataaaataaa ggcaatgcca cattctccat
      601 ggcattttta aaggagatca ggttgaaaga ctgaaacaca aagcctatca ttttgttgcg
      661 gtatatcgcc gccttttttt cactcaggcc ggcaattaat ttaccgtcca gtaaatacct
      721 gcccgtatcg taatcgtcca atatgcccaa tacatttaat agggttgatt ttcctgaacc
      781 tgaggccccc atgatggcta cgtactctcc ctgacttatg ctaaggtcaa tgtctttcaa
      841 cacatgcaga ggctgtactc cgggatacgt tttgttgagt ttttctaaga ggatcattcc
      901 gccgatagac ttacttaaat aaaacggcag ttgcccctca acaggcaact gccgcataaa
      961 aatcgtttat tcattattaa aagacataac ggactgctcc ggcatagccc cagtaaggac
     1021 cgaaattgcc aaacagccac atgggacggc agtcgatggc gagctgaagg ggaataccgg
     1081 gaaatgtata ttccacacca atttgtccgc caatacctgc tgaaatgcct tcataattat
     1141 tgtttccttc attgtttccg aatccaaagt aaagatcggc tccgggtcct gcataccatt
     1201 taaaaccttc gggaagtcct attgattcca ggctccacac ccagtgatac actgcagcaa
     1261 ggttcagtcc gttgaaaact cccaggccaa ggtcggcttc caaacggttg gcatcgccta
     1321 aagcttgttg gtatgagatt tcaaatccat aaccgccgcg aagaccaaga gccttgcttt
     1381 gtgcaaaagt agctgtaaaa cccaaaagac aaacggctaa tgcaagaata gtttttttca
     1441 taactaaatt cagtattgag tgagactaat aaaatactct ttttcctcta atgaaaaagc
     1501 ggcacaaaag tagctaagaa aacttgcatt ggcatataca gatacgttaa caagtatgaa
     1561 tattaatgtt ttttcttttt aaatatctag tattcagctt ataaaattat tagcaaaaat
     1621 aaatacagaa aaatttattt atttgcatgt atttgtgcga aggttggaaa aatagttgta
     1681 atttcgcggc cgaatttgtt ccgctttgaa aataatgatc attttcaggc ttaaaacttt
     1741 tctgaaaaca gtgggtgatc cgactaaaaa taacactatg acagatgtaa ttaaaattaa
     1801 gaaaggcttg gatatcagtc ttaaaggtgg agcttttccg gaagttcagt ctgcgtcgta
     1861 tacggaaaat tatgcgctct gtccgactga ttttcacgga ctgataccca gggttctggt
     1921 taaggaggga gagcaggttt tggccggaac tccccttgtg gcagacaaaa acaagcctga
     1981 gattcttttt gtcagtcccc tgagtggtgt ggtaagtgca gttaaaaggg gtgaaaaacg
     2041 cagattactg gccatcgaaa tccgtgccga ccaaaaaaac gctgccttga gttatcctgt
     2101 gcgcaatcct ttggatatgg acggagttaa agtgaaagaa gctcttttgg aagccggcct
     2161 ttggccctat attaaaagga ggccttacga tgtgattgcc aatccccgtg tggagcccaa
     2221 agccatcttc ataagcgctt ttgatactgc tcccctggcg cccgattatg cttttattct
     2281 caaaggccag ggccatgatt ttcagacagg gttgaatgcc ctgaccaaat tgaccaaggg
     2341 taaagtacat ttaagcatac ccgccggttc cggttcgggc gaactcaaga acgctcagaa
     2401 cgttgaaatt catcgtttcg aaggcccgca tcccgcaggt aatgtc
//
LOCUS       DAOSGL010000325         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_42819_length_2413_cov_3.508906, whole genome shotgun sequence.
ACCESSION   DAOSGL010000325 DAOSGL010000000
VERSION     DAOSGL010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42819_length_2413_cov_3.508906"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKY68_08720"
     CDS             complement(<1..189)
                     /locus_tag="PKY68_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25148.1"
                     /translation="MGLLLLTVLLTLSCEMEDRPETNGDLPGLDQGACRGLWILSEGL
                     MNLNNSTLAYYSFEQKSLN"
     gene            complement(239..2284)
                     /locus_tag="PKY68_08725"
     CDS             complement(239..2284)
                     /locus_tag="PKY68_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH25149.1"
                     /translation="MKKAISFSLEAKPVLICLAFALSVVLPGGTALYALTDPVAQEYT
                     IEEITVLGRESKAKVQEGKLVQEMDSSLFQALPFLQLADALKFFTGVSVKDYGGLGGL
                     KTISVRSLGAHHTALSYDGLPVTDVQNGQIDLGRFALDALESISLSTGQTEDIFQPAR
                     LFASAGLLQLQSLKPGFAPGKKQNLRLKFQGGSFGLLNPSLLLQRKLNDRHSLSLDVY
                     YLQSRGDYPYLMYYGSEGDLVSRERRRNSDIRQFRLEPSWYWRNTKGSEATVKLYYNQ
                     SERGLPGAGIYYNTENSSGQRLWDKQSFIQGHYKSLLHPSWSLRLNTKINGSYTHYLD
                     PSFLNSDGRDEAEYRQMESYLSGALQYRPQSAFSLVFSQDISMQELFSEEFLVNRPQR
                     LSLYTVLAGKASAGAWNLSGNLLFCRIMEEAGAGPQGADYSKLTPSVSVSLRLVQGWY
                     VRAFYKSIFRMPSFNDLYYGRIGNPDLIPENTRQLNLGTGLSTKPLPGQELSLSLDVY
                     ANRVENKIVAYPTQNVFIWTMLNLGEVSIKGADFSLDWACSLGKNAALALRASYTLQS
                     VLNVTDPESRAYGHQLPYTPKHSGSGVLLLRAFDWQLSYNLLYSGIRYSGFENYALNR
                     MPAYADQGISLNKSFNLAKTKMTLGAEVLNLTGKQYEIIRNYPMPGRSFRAKLVFSR"
BASE COUNT          655 a          617 c          547 g          594 t
ORIGIN      
        1 attcaagctt ttctgttcaa acgaataata agccagggta gaattattta aattcatcag
       61 gccttcggac aaaatccaaa gcccacgaca agcaccctgg tccaggcccg gcaaatcgcc
      121 gttggtttcg ggccggtctt ccatttcaca tgaaagggtc agtaaaacgg tcagcaagag
      181 caagcccaaa actgcaagtc ttgtaactaa tgccaaaaca gcaggcagaa atctttgttt
      241 accgactaaa aacaagcttc gccctgaaag aacgtcccgg catggggtaa tttcgaatga
      301 tttcatactg ttttcccgtt aaattgagca cttcagcacc cagcgtcatt ttggtcttag
      361 ccaaattaaa agacttgttg agcgagattc cctgatcggc ataggccggc atacgattca
      421 aagcataatt ttcgaagccc gaatagcgaa tgcccgaata aagcaagtta tacgataatt
      481 gccagtcaaa ggcccgtaga agcaaaacgc ccgatccgct gtgtttgggt gtataaggca
      541 attgatgtcc gtaggccctg ctttcgggat cggtcacatt caaaaccgac tgcaaggtat
      601 agcttgctct aagcgcaagg gccgcgttct tacccaaaga gcaagcccaa tccagactaa
      661 aatccgcgcc ttttatgctg acttctccca aattcagcat ggtccatata aaaacatttt
      721 gcgtagggta ggccacaatt ttgttttcta cccggttggc atacacatcc aggctaaggc
      781 taagctcctg tccgggtagg ggttttgtac tcaggccggt acccaggtta agctgccggg
      841 tgttttcggg aatcagatcg ggattaccga tacgtccgta atacaaatcg ttgaaactgg
      901 gcattctgaa tatagattta tagaaagccc tcacgtacca gccctgtaca agcctcaacg
      961 acaccgaaac cgaaggtgta agtttgctgt aatctgctcc ctgaggcccc gctcctgctt
     1021 cttccataat ccggcaaaaa agcaaattgc cgctcaaatt ccaggcgcct gccgaagctt
     1081 tcccggccaa cacggtgtat aaactcaggc gctgaggacg gtttacaaga aactcttctg
     1141 aaaaaagctc ctgcatgctg atatcttgcg aaaaaaccag gctgaatgcc gattggggcc
     1201 tatactgtag agctccgctc aggtaggact ccatctgccg gtactctgct tcgtcccgac
     1261 cgtcggaatt caaaaaggac ggatcgaggt agtgggtata actgccgttt attttggtat
     1321 tcaatcgcag actccaactc ggatgcaaaa ggcttttgta atgcccttgt ataaaactct
     1381 gcttatccca tagcctttga ccggaagaat tttccgtatt ataatagatg ccggctccgg
     1441 gcaaaccccg ctccgattga ttgtaataga gtttaactgt ggcctcggaa cctttggtat
     1501 tgcgccaata ccaggaaggc tccagcctga attggcggat atccgaattc cggcgacgtt
     1561 ctctgctgac cagatcccct tcggaaccat agtacattaa atagggataa tctccccgcg
     1621 attgcaaata ataaacatcc aggctgagag agtgccgatc gttaagctta cgctgcagca
     1681 acaaagaggg attcaacagg ccaaaagagc cgccttgaaa tttcaatctc aggttttgtt
     1741 ttttaccggg agcaaaaccg ggtttcaggc tttgaagctg caaaagaccg gctgaagcaa
     1801 acaaacgggc aggctgaaat atatcttcgg tttgtccggt actcaggctt atgctttcca
     1861 gggcatccaa ggcaaaacgt cccaaatcta tctgcccgtt ttgcacatcc gtcaccggca
     1921 agccgtcgta ggaaagggct gtatgatgcg cccccagact gcggacggag atggttttga
     1981 gtccgcccaa tccaccgtaa tccttcacac tgacaccggt gaaaaatttc aaggcatcgg
     2041 caagctgcaa aaatggtaat gcctgaaaaa gactgctgtc catttcctga acgagtttgc
     2101 cctcttgtac ttttgctttg gattcccgtc ccagcaccgt tatttcttca atggtatatt
     2161 cctgagccac aggatcggtc agggcataca atgcagtacc ccctggcaaa acaaccgaca
     2221 gcgcaaaggc taagcaaatc aacacgggct tagcttccaa cgagaagctg attgcttttt
     2281 tcatcttacg gtaaattcag cgatccggcg tcgcatgttg gtccatgccg aaaaaaagga
     2341 aagcctcttg acggcatatt cagcaaacag gttttctgac ttggtctgtt ttcccgacct
     2401 tcccatccat aag
//
LOCUS       DAOSGL010000326         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_42823_length_2413_cov_3.225615, whole genome shotgun sequence.
ACCESSION   DAOSGL010000326 DAOSGL010000000
VERSION     DAOSGL010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42823_length_2413_cov_3.225615"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PKY68_08730"
     CDS             <1..241
                     /locus_tag="PKY68_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25150.1"
                     /translation="AVPFLIYRSDAGSAQARPAKGPDSSFSPGPGVLEPSGKLFVPDA
                     VQVFDEFSVREGFYGTLQGEEFIRAFFGLSTKADP"
     gene            308..1624
                     /gene="thrC"
                     /locus_tag="PKY68_08735"
     CDS             308..1624
                     /gene="thrC"
                     /locus_tag="PKY68_08735"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOH25151.1"
                     /translation="MNYYSTNKQAPPVSLQEAVVRGLAPDRGLYMPERINTLPASFFE
                     ELPGLSFQEMSYRVAMAFFGEDLPEDTLRELVYDTLSFDTPLVRVDERIYSLELFHGP
                     TLAFKDVGARFMARLLAYFIRRQGQEKVHVLVATSGDTGSAVANGFLGVEGIQVHVLY
                     PKGLVSEIQEKQFTTLGQNITALEIEGNFDDCQRLVKQAFMDTELNRRFHLTSANSIN
                     VARFLPQAFYYFNAYAQWKRYNDPSKLVFCVPSGNFGNITAGFFAWRMGLPVERFIAS
                     NNRNDVFYEYLQSGHYRPRASVATLANAMDVGDPSNFARILDLYGHSHEAITAEIAGA
                     RYDDQEIRDTVHRTFKEKNYLLDPHGACGYRALEEGLKPGESGIFLETAHPAKFKDTV
                     EDILGEAIVVPKRLQAFMQAKKQSIPLSSAFDDFKAYLMQQKGFNK"
     gene            1662..>2413
                     /locus_tag="PKY68_08740"
     CDS             1662..>2413
                     /locus_tag="PKY68_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOH25152.1"
                     /translation="MEQSSMYRPETSGQGLREFTLRALLIGLVLAVVLGAANAYLGLK
                     AGMTIAATYPAAVISMAVLRFFKGSLLEENFARTVGSIGESVAAGAIFTLPAFFIAGV
                     WGGEEFSSWSSYMTASLILVTGGVLGVLFVALLRRVMVEDKELPFPESTAAAEIHKSG
                     RSSTGDSKYLVWAMVGGALVTLGSALKLFASSAKAFFKVAIAKLTSGHTIEGGFYAAA
                     PNVSPAFLGVGYIIGPRLASLNFSGSVIAWCS"
BASE COUNT          536 a          597 c          652 g          628 t
ORIGIN      
        1 ggcggttccc tttttgattt atcgcagcga tgcaggttct gcgcaagccc ggcctgccaa
       61 aggcccggac agctctttta gcccggggcc cggcgttttg gagccctccg gcaagctttt
      121 tgtccctgac gctgtacaag tcttcgatga attctcggtg cgggagggct tttacggcac
      181 gctgcagggg gaggaattta tccgtgcctt tttcggcctg agtacgaaag cagaccctta
      241 attacaaaga aggaagggtt tagatgttga attaacacag tcagacattt ttttcgatca
      301 acaaataatg aactactaca gcaccaacaa acaagctccg ccggtctctt tacaagaagc
      361 cgtggtcagg gggctggctc ccgaccgggg tttgtacatg cccgagcgca tcaacacttt
      421 gccggcttcg ttttttgaag agcttccggg cctgagcttt caggaaatgt cttaccgggt
      481 ggccatggcc ttttttggag aagatcttcc cgaagatacg ctccgggagt tggtttacga
      541 cacattgagt tttgatacgc ctttggttag ggttgacgag cgtatctaca gtttggagtt
      601 gtttcacggc ccaaccctgg cgttcaaaga cgtaggcgcc cgttttatgg cccggttgct
      661 ggcctatttt attcgccggc aggggcagga gaaagtccat gtgttggtag ccacctcagg
      721 cgataccggc agtgccgtgg ccaatggctt tttgggagtg gaaggcattc aggtgcatgt
      781 tttgtatccc aaaggcctgg tcagcgaaat tcaggaaaag cagtttacca ccttggggca
      841 aaatatcacg gccctcgaga tagagggcaa cttcgacgat tgccaacgtc tggtcaagca
      901 ggcctttatg gatacggagc ttaaccggcg ctttcatctg acttcggcca attccatcaa
      961 tgtggctcgt tttctgcctc aggctttcta ttatttcaat gcctatgctc aatggaagcg
     1021 ttataacgat ccttcgaagc tggttttttg cgtccccagc gggaatttcg gtaatatcac
     1081 ggccggcttt tttgcctggc gtatgggatt gcccgtcgaa cgttttattg cttccaacaa
     1141 ccgcaacgat gttttttatg aatacctgca aagcgggcat taccggccac gggcctcggt
     1201 agccactttg gccaacgcca tggacgtggg cgatcccagc aattttgccc gcatcctcga
     1261 tttgtacggg cattcccatg aggccattac tgccgaaata gccggagctc gctacgacga
     1321 tcaggaaatc cgggatacgg ttcaccgtac ttttaaagaa aagaactacc tgctcgatcc
     1381 ccacggtgcc tgcggctaca gagccctgga agagggcctg aaaccgggcg aaagcggcat
     1441 ctttctcgaa acggcccatc cggccaaatt caaagatacg gtcgaggaca tactgggcga
     1501 ggctatagtt gtgccaaaac gcctgcaggc ctttatgcag gcaaaaaaac agagcattcc
     1561 cctaagctct gccttcgacg actttaaagc ttatttgatg caacaaaaag gctttaataa
     1621 ataaaaaatg ctacattagc ggcgctgaat tttttaatac tatggaacaa tcttcaatgt
     1681 atcgccctga gacttcgggt cagggactca gagaatttac gctaagagcc ttgcttatag
     1741 ggctggttct ggcggtagtg ctgggagcag ccaacgccta cttaggcctc aaggcaggca
     1801 tgaccattgc tgccacctat ccggcggccg taatcagtat ggctgtatta cgctttttta
     1861 aaggcagtct gttggaagaa aattttgccc gtacggtagg atccatcggt gaatcggtgg
     1921 ctgccggggc tatttttact ctgccggcct tttttattgc cggtgtatgg ggtggggagg
     1981 aattttcttc ctggagcagt tatatgacgg cttcgctcat cctggttacc ggcggtgtgc
     2041 tgggagtgct ttttgtggct cttttgcgca gagtaatggt cgaagacaag gaattacctt
     2101 ttcccgaaag taccgcagca gccgaaattc acaaaagcgg acgcagcagt acgggcgatt
     2161 ccaaatacct ggtttgggct atggtgggcg gagctttggt aacgttgggc tctgctttga
     2221 agctctttgc ctcgagcgcc aaggcttttt tcaaggtggc catagctaaa ttaaccagcg
     2281 gacacaccat cgaaggaggt ttttacgccg ccgctcccaa tgtcagcccg gcctttttgg
     2341 gcgtgggtta catcatagga ccaagactgg cctcgctgaa tttcagcggc tcggtaatcg
     2401 catggtgctc gat
//
LOCUS       DAOSGL010000327         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_42863_length_2411_cov_3.321307, whole genome shotgun sequence.
ACCESSION   DAOSGL010000327 DAOSGL010000000
VERSION     DAOSGL010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42863_length_2411_cov_3.321307"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1107)
                     /locus_tag="PKY68_08745"
     CDS             complement(49..1107)
                     /locus_tag="PKY68_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOH25153.1"
                     /translation="MKTMQAAYLPGNSTVVLKEVEIPVPGHGEVLIKMKSSTICGSDI
                     RAIYREHLGKGPEGYQNKICGHEPSGQIVKCGPGLRRFKEGDRVVVYHISGCGLCNDC
                     RRGYMISCTSPYRRAYGWQRDGGMAEYLLAEEKDLVLLPDSLTYTDGAQIACGFGTVY
                     EGLEKIGISGNDAVLVVGLGPVGLASLMLAKAMGANKLIGVDTVQERLDIAKEKGLAD
                     AVFLSDADALKKIQDLTGGNGVERAVDCSGNTYGRQLAVRATRKWGKIVLIGEGGTME
                     LNPSPDLIHDQKAVYGSWVTNIWRMEELVERIVRWNIHPEDLVTHRFKLDNASEAFAL
                     MAEGKCGKVAVVFDEEIR"
     gene            complement(1183..2199)
                     /locus_tag="PKY68_08750"
     CDS             complement(1183..2199)
                     /locus_tag="PKY68_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH25154.1"
                     /translation="MNKEMKCVDPRSVPTVLLATGDKIPVIGMGTFGSDNYGAEEIAE
                     AVRVAIKMGYRHLDCAAVYGNEKEIGDVLRELFQEGVVKREELWITSKVWNDKHHDVK
                     GACKDSLKKLGLDYLDLYLVHWPFPNHHPPMVDVESRDPHARPYIHEEYMAVWKAMEQ
                     LADDGLVRNIGTSNMTRAKMELLLRDCRIKPVCNEMELHPHFQQPELKKYFEDQGITV
                     IGFSPIGSPNRPERDKTPEDTVPIEDPVIVEIARKHGVHPAVICIKWGAQNGQIPIPF
                     SVKPEKLFSNLLAVVQDPLSAEEMEAIREQDKNCRFIKGQVFLWPGARSWEDLWDLDG
                     IIRQ"
     gene            complement(2211..>2411)
                     /locus_tag="PKY68_08755"
     CDS             complement(2211..>2411)
                     /locus_tag="PKY68_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH25155.1"
                     /translation="IALDGLGEDAKIGASGLIMAILGGALLTPLQGLVSDLAGINIAY
                     SVPLICFVVVMAYGIMAQKVGK"
BASE COUNT          617 a          651 c          498 g          645 t
ORIGIN      
        1 tctttgtact cgagaaaaag taatttcagc gagtttccaa tctcgatctc aacgaatctc
       61 ttcatcaaaa accacggcca ccttgccgca tttaccctcg gccatcaagg caaaggcttc
      121 agatgcgttg tcaagcttga aacgatgcgt taccaggtct tcgggatgga tattccaacg
      181 aacaatacgc tcgaccaact cttccatacg ccagatattg gtaacccagg aaccgtaaac
      241 ggctttttga tcgtggatca ggtccggact gggattcaac tccatagtgc caccttcgcc
      301 aatcaaaaca attttacccc atttacgtgt ggcacggacg gctaactgac ggccgtaagt
      361 attgcccgaa caatctactg cacgctctac tccgttacct ccggtaagat cctgaatttt
      421 ctttagcgcg tcggcatccg acaaaaagac ggcatcggcc agaccctttt ctttagctat
      481 atccagacgc tcctgcacgg tgtctactcc aatcagctta ttggcaccca tagccttggc
      541 cagcatcagc gaggccagac ccacggggcc aagaccgact accaaaacag cgtcgttgcc
      601 cgaaatgcct attttctcca agccttcata aacagtgcca aaaccgcagg caatttgtgc
      661 cccgtcggta taagtaaggg aatcgggcaa gagaaccaaa tccttttctt cggccaacag
      721 gtattcggcc atacctccgt cgcgctgcca gccataagct ctccgatagg gactggtaca
      781 actgatcata taacctctac ggcaatcgtt gcacaatccg caacccgaaa tatgatagac
      841 aaccactctg tcgccctctt taaaacgacg taaacccgga ccgcatttca cgatctgtcc
      901 cgagggttcg tgtccgcata tcttgttttg atagccctcg gggcccttgc ctaaatgttc
      961 tctgtaaata gcacggatat cgctgccaca aatggtggat gatttcattt taatcaggac
     1021 ttcgccgtgt ccgggaacgg ggatttctac ctctttaagt accacagtac tgttgccggg
     1081 cagataagct gcttgcattg ttttcataag ccttacactt cttgcttttt ttatcttaat
     1141 tctatataaa ccgaatgctc catcgcatgt tgcattccat gtttattgac gtataatgcc
     1201 gtctaaatcc cagagatctt cccaggaacg ggctcccggc caaaggaaga cctgaccttt
     1261 tataaaacgg caatttttat cttgttcgcg gatggcttcc atttcttcgg cactcagagg
     1321 gtcttgtacc actgccaaga gattactgaa cagtttttcc ggttttaccg aaaaaggaat
     1381 gggaatttgt ccgttctgag ccccccattt aatgcagatt actgccggat gtactccgtg
     1441 tttgcgagct atctcaacga taaccggatc ttcgatggga acagtatctt cgggtgtttt
     1501 gtccctctcc ggacggttgg gagaaccaat cggcgagaaa cctatcacgg ttatgccctg
     1561 atcttcaaaa tactttttca attcaggctg ttgaaaatgg ggatgcaatt ccatttcgtt
     1621 gcagaccggt ttgattctgc aatcgcgcag cagcaattcc attttggccc gggtcatatt
     1681 ggaagtaccg atattacgca ctaaaccatc gtcagcaagt tgttccatgg ccttccatac
     1741 agccatgtat tcttcgtgaa tataaggacg ggcatgggga tcgcgcgatt ccacatcgac
     1801 catgggagga tgatgattgg gaaagggcca gtgtaccaga tacaagtcca ggtaatccaa
     1861 acccaatttt ttcaaagaat ctttacaggc tcccttgaca tcatggtgct tatcgttcca
     1921 tactttggaa gtaatccaga gttcttccct tttgacgacc ccctcctgaa acaattcccg
     1981 cagcacatca cctatttctt tttcgttgcc gtaaactgca gcacagtcta aatgcctgta
     2041 acccatttta atagctactc tgaccgcctc agcaatttcc tcagccccgt agttgtcaga
     2101 gccgaaagtc cccatgccga tgacaggaat tttgtcgcct gttgccagaa gtacggtagg
     2161 cactgaacgg ggatctacac atttcatttc tttattcata aaaatcttct ttattttccg
     2221 actttttgag ccattatgcc gtaagccatc actactacaa aacaaataag aggtacacta
     2281 taagctatat tgatcccggc caaatcggaa accagtccct gtaaaggggt caacagggct
     2341 cctccgagaa tggccatgat caatcccgaa gcaccgatct tggcatcctc gcccaaacca
     2401 tccagggcaa t
//
LOCUS       DAOSGL010000328         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_43609_length_2384_cov_4.787892, whole genome shotgun sequence.
ACCESSION   DAOSGL010000328 DAOSGL010000000
VERSION     DAOSGL010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43609_length_2384_cov_4.787892"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(439..1290)
                     /locus_tag="PKY68_08760"
     CDS             complement(439..1290)
                     /locus_tag="PKY68_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25156.1"
                     /translation="MKVHEILIKLFETFLSIIRIIRHTSFRGIGKIARTSNTCLIMGN
                     GPSLTSSLEKNKDKLKKTDLLAVNFMGLTPEFMIYRPNLYVLCDPGFWIESPDTVLGE
                     KVRKLYQVLADTTNWDLQLYLPHDARKSKFIKAILSSNKFIHVIYFNKTKIEGLRCFT
                     YFTYSAQWGMPRAQNVLCAALMLCIYSGYKTIYLAGAENDWIKNLWVDANNRLRLDDL
                     HFYDNTNQGNAIVLNTKMYEQFLSWYYAFKSYAEIAAFAHNRGINVINTNLTSFIDVF
                     EKQELID"
     gene            complement(1301..1492)
                     /locus_tag="PKY68_08765"
     CDS             complement(1301..1492)
                     /locus_tag="PKY68_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25157.1"
                     /translation="MSLTSVYRRLFLETSKSLRLFSGLLRARYKQKGLKATRGMQSYP
                     CTFMTPEKQAIIDAINKYC"
     gene            complement(1500..>2384)
                     /locus_tag="PKY68_08770"
     CDS             complement(1500..>2384)
                     /locus_tag="PKY68_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOH25158.1"
                     /translation="SDASYWKKRVKDIRGVSEETTTGVHRLYQMLEAKELLFPAFNVN
                     DSVTKSKFDNLYGCRESLADGIKRATDVMLAGKVVVVCGYGDVGKGCAHSMRSYGSRV
                     IVTEIDPICALQAAMEGFEVKTVEESLDEGNIYVTTTGNCDVITIDHMRKMKDNAIVC
                     NIGHFDNEIQVDKLKHYPGIKRINIKPQVDQYFFPEGHSIILLADGRLVNLGCATGHP
                     SFVMSNSFSNQTLAQIELWSNRHEIAVYRLPKKLDEEVARLHLEQIGVKLTKLNKKQA
                     DYLGVPIEGPYKAEHYRY"
BASE COUNT          656 a          489 c          515 g          724 t
ORIGIN      
        1 tcgaacgatt cgaaagaggt aattagttca attttttcgg gattatggga atcatattca
       61 aaacccgcgg ataacaaatt gggtgcgtat aaatcgtgcc aataaaaatt atagccttta
      121 tccctcatca ggcggacgaa caggccgtaa cctcctgcca tatccaaata tgacgccttt
      181 tttgtgaaaa aaagtctgat cagactcctt gtcattctgg aacagagcaa atttctctgg
      241 accagcccgg tatcggtggt atttatcgga ttcttataag actcttcaag ccaatatgct
      301 tgttcggttt gagtaaaatt acagtgagga cagttgtagt agctgattat atatttccct
      361 aaaatttctg ccttaaatgc gaaaagggtt tctttttggc atatcttaca aacaggcata
      421 tcctttataa taaaagcatc aatctatcaa ttcttgtttt tcaaatacat caataaagga
      481 ggtaagatta gtattaatca catttatccc ccggttgtgg gcaaaggccg caatttctgc
      541 ataactctta aaggcgtaat accaggataa aaattgctca tacattttag tgttaagaac
      601 aatggcatta ccctgatttg tattgtcgta aaaatgaagg tcatccagtc gcagcctatt
      661 attggcatca acccaaaggt tttttatcca gtcgttttct gcaccggcaa gataaatagt
      721 tttgtatcct gaataaatgc aaagcatcaa agcggcacaa agcacatttt gtgcacgagg
      781 cataccccat tgagctgaat aagtaaaata tgtaaagcat ctaagtcctt caatttttgt
      841 tttattgaaa tagatgacat gaatgaattt gttagatgaa agaatagcct tgataaattt
      901 gctttttctg gcatcgtgcg gaaggtataa ctgtaaatcc cagtttgttg tgtcggccag
      961 tacttgatac aatttcctta ctttttcacc aagtactgtg tcgggggatt cgatccagaa
     1021 accgggatca cataaaacat acagattagg cctgtatatc ataaactcag gcgtgaggcc
     1081 cataaagttt accgctaata aatctgtttt tttgagctta tctttgttct tttccagaga
     1141 agatgtcagt gaggggccat tccccataat caggcaagta ttagatgtac gtgctatttt
     1201 cccgattcct cgaaaggaag tatgccggat gattcgaatt atcgacagaa acgtctcaaa
     1261 cagtttaatg agtatttcgt gcactttcat caacatagag tcagcagtat ttatttattg
     1321 catcaataat agcctgtttt tcgggagtca taaaggtaca gggataactc tgcatccctc
     1381 tggttgcttt gagtcccttt tgtttgtaac gggcacgaag caatccgcta aacagacgca
     1441 aagatttgga ggtttcaaga aacaatcgac gatataccga agtcaggctc attgtataat
     1501 caatatctgt aatgctccgc tttatagggt ccttcgatgg gtacgcctaa gtagtctgcc
     1561 tgttttttgt tgagtttggt cagttttact ccaatctgtt cgagatggag ccgggcgacc
     1621 tcttcgtcga gttttttggg caggcggtaa acggcaattt catgccggtt gctccataat
     1681 tcaatttggg ccagggtctg gttgctgaac gaattgctca ttacaaacga tggatgacca
     1741 gtggcgcaac ccagattgac caggcggccg tcggccaaca aaatgatgga atggccttcg
     1801 gggaaaaagt attgatcaac ttgaggttta atattgatcc gcttaatgcc gggataatgc
     1861 ttgagtttgt ccacctgtat ttcattgtcg aagtgaccta tgttacagac gatagcattg
     1921 tctttcatct tgcgcatgtg gtctatggtg atgacatcgc aattgccggt ggtagtaacg
     1981 taaatattgc cttcgtcgag gctttcttcg acggttttta cctcgaaacc ttccatggcg
     2041 gcctgcaagg cgcaaatggg atcgatttcc gtcacaatta ctcttgatcc atagcttcgc
     2101 atggaatggg cgcaaccttt gcctacatcg ccgtagccac aaactaccac caccttgccg
     2161 gccagcataa catcggtggc tctttttatt ccgtcggcca gggattcacg gcagccgtaa
     2221 aggttgtcaa atttcgattt ggtcaccgaa tcgttcacat tgaaagcggg gaaaagcaat
     2281 tctttggctt cgagcatttg gtagagacga tgaacgccgg tggtggtttc ttccgaaaca
     2341 ccgcggatat cttttacccg tttcttccag taagaggcat cgga
//
LOCUS       DAOSGL010000329         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_43741_length_2380_cov_3.305806, whole genome shotgun sequence.
ACCESSION   DAOSGL010000329 DAOSGL010000000
VERSION     DAOSGL010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43741_length_2380_cov_3.305806"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..549
                     /locus_tag="PKY68_08775"
     CDS             100..549
                     /locus_tag="PKY68_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH25159.1"
                     /translation="MKFRSILLPTLFCLLSLNSYSERIVRIDGQELQQTIGRISFKGD
                     TAVLHFSDTDSLSTHCLLLSIEFTEGSGLSPLQNTRVLLARNPVKDILPLENLQAGSR
                     LLLYDIEGRLVKTQQAHASSVQIDLSDLPAGVYLLRTGKYLVRFVKL"
     gene            561..>2380
                     /locus_tag="PKY68_08780"
     CDS             561..>2380
                     /locus_tag="PKY68_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOH25160.1"
                     /translation="MKKMLTLALLVVVLTGLQANTKLRVDLRDTDSVEHFSLEELESI
                     VFGENNEFSIYFKQGDSLHFPGTASGIHFVQEPDSTPNTIAIVSTEGWLEAASVSWEP
                     LESALSYRVYCKSQETGSEYWPLDSPLIRNYGSYGRADAIGLKAGYYRFKIVPLDREG
                     LEMDDFAAESDFVEVRAHDRSGFAHHNLNEGIGAYKNDGSLKDNALVFYVTKDNCNSI
                     THNVIIDSKGKTETRTGIGKILQGKQKGHDSTPWAVRFIGTIRTEDFEAGQLLSDQDG
                     LQVKSDAGAAPLNVTLEGVGNDATAYGWGLTLYRGSSIEVRNLGLMNFQEDGISIKSS
                     QHVWVHHCDIFYGSPGSASDQNKGDGSLDLKDNCQYATFSYNHFWDAGKASLCGMTGE
                     SGENWISYHHNWFDHSDSRHPRVRVMSVHVYNNFYDGISKYGVGATMGASVFVEANYF
                     RNTKRPMMISLQGTDATGDGTFSGENGGMIKSFGNVFAETPNLQFIPHTQSATSFDAY
                     EAASRQDSVPSSYKSLVGSHTYNNFDINETLMYTYSPHPAEEVPEIVCGPLGAGRMGK
                     GDFTWSFDNAVDASYAVNEALKAAIASYSSSLIGFFGQAG"
BASE COUNT          620 a          618 c          605 g          537 t
ORIGIN      
        1 ctgaaatttg tgagaagaac cgaggcttgt ggagtattga ggccgaaaac tacttgctgg
       61 aaaactgcgc aagtcatccg aaaaaataaa tcttcaacca tgaaattccg tagcattctg
      121 ctgccgaccc tgttttgcct tttaagcctg aattcctatt cggaacgaat agtacggatc
      181 gatggtcagg aattgcaaca aacgatcggt cgcatcagct tcaaaggcga cacggctgtg
      241 ttgcattttt cagacacgga cagcctgagt acccattgtc tgttgttgag cattgaattt
      301 acggaaggaa gcggactcag tcccctgcaa aatacgcgtg ttttattggc ccgcaatccg
      361 gtaaaggata tcttgccgct ggaaaacctc caagccggca gtcgtctgct gttgtacgat
      421 atcgaaggcc gcctggtcaa aacacaacaa gctcacgcaa gctcggtaca gatagaccta
      481 agcgatctgc ctgccggcgt ctatttgctc aggacgggca agtacctggt tcgttttgtc
      541 aaactttaga ttcgcttgtc atgaagaaaa tgctgacact tgccttgctc gtagttgttt
      601 tgaccggatt gcaggcaaat accaaactgc gtgtcgatct tcgtgacaca gacagcgtgg
      661 agcatttcag cctggaagag ctcgaaagca tagtttttgg cgaaaacaac gagttcagta
      721 tttatttcaa acagggagac agcctgcatt tccccggtac tgcatccggc attcattttg
      781 tgcaggaacc cgattcaact ccaaacacca tagccatcgt ctcgaccgaa ggctggctcg
      841 aagcggcttc cgtaagctgg gaacctttgg aaagcgctct ctcctatcgg gtctattgta
      901 aatcgcagga aacgggaagc gaatattggc ctttggacag cccgctgatc cgcaattacg
      961 gcagctacgg cagagccgac gccataggct tgaaagccgg ttattatcgt tttaaaatcg
     1021 ttcccctcga ccgggaaggt ttagagatgg acgattttgc ggccgaatcg gactttgtgg
     1081 aagtaagggc ccacgatcgc agcggctttg cccaccacaa tctgaacgaa ggtatcggcg
     1141 catacaaaaa cgacggcagc ctcaaagaca atgccctagt attttacgtg accaaagaca
     1201 actgcaacag catcactcac aacgtgataa tagattctaa agggaaaacc gaaacgcgta
     1261 ccggcatagg aaaaatttta caaggcaagc aaaaaggcca cgactccacg ccctgggcag
     1321 tgcgttttat aggaaccatc cgcacggaag attttgaggc cggtcaattg ttgagcgatc
     1381 aggacggcct gcaagtgaaa agcgatgccg gagccgctcc tttgaacgta acgctcgaag
     1441 gtgtcggaaa cgatgccaca gcctatggct gggggctcac cctgtaccgc ggcagcagca
     1501 tcgaagtgcg caacctgggg ctgatgaatt ttcaggaaga cggaatctcc atcaaaagca
     1561 gccaacatgt ttgggtacat cattgcgata ttttttacgg cagtccgggc agtgcctccg
     1621 atcaaaacaa gggcgacggc tcactcgatc tcaaagacaa ttgccaatac gctacgtttt
     1681 cctacaatca tttctgggat gcaggcaaag cctcgctttg cggcatgacc ggcgaatcgg
     1741 gcgaaaactg gatcagttat catcacaact ggttcgacca ttccgattcg aggcatcccc
     1801 gtgtacgcgt tatgtcggtg cacgtgtaca acaacttcta cgacgggatt tccaaatacg
     1861 gtgtgggggc caccatgggg gccagtgttt ttgtcgaagc caattacttc cgcaatacca
     1921 aacggcctat gatgatttct ctccagggaa cagatgccac gggcgacggc actttttcgg
     1981 gcgagaacgg aggaatgatc aagagctttg ggaacgtttt tgccgaaacc ccaaatctcc
     2041 agttcatccc ccacactcag tccgccacca gctttgacgc ctacgaggct gcttccagac
     2101 aggacagcgt gccttcttct tacaaaagcc tggtgggcag ccatacctac aacaatttcg
     2161 atattaacga aaccctgatg tatacctaca gccctcaccc ggccgaagag gtgccggaaa
     2221 ttgtttgcgg tccgcttggc gcgggacgca tgggcaaggg tgatttcacc tggagcttcg
     2281 acaacgccgt cgacgcctct tacgccgtga acgaagcgct caaagcggcc attgcctcct
     2341 acagcagcag tctgataggc ttcttcggtc aggcagggaa
//
LOCUS       DAOSGL010000330         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_43855_length_2376_cov_3.211116, whole genome shotgun sequence.
ACCESSION   DAOSGL010000330 DAOSGL010000000
VERSION     DAOSGL010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43855_length_2376_cov_3.211116"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKY68_08785"
     CDS             <1..231
                     /locus_tag="PKY68_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOH25161.1"
                     /translation="MSSAYAARFTILPVEINASSRAVTWLNKTGITNVSTHPMAVDAL
                     KWAAYTYVIAALSSLATLIYYILIFLGGRNRN"
     gene            238..1614
                     /gene="hisS"
                     /locus_tag="PKY68_08790"
     CDS             238..1614
                     /gene="hisS"
                     /locus_tag="PKY68_08790"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOH25162.1"
                     /translation="MAIQKPSIPKGTRDFTPEETAKRNYIFNTIRSAFESYGFQAIET
                     PALEQLSTLMGKYGDEGDKLLFKILNSGDFAGKLSDQELLSRETGRLTRLISEKGLRY
                     DLTVPFARYVVMHRNEIVFPFKRYQIQPVWRADRPQKGRYREFFQCDADVVGSDSLLN
                     EVELLQLLDTVFNDLGIRVCIKLNNRKILAGLAQILGQEERLTDITVAIDKLDKIGLD
                     NVRAELVEKGIPAQTVRELEPVLLLQGSNSEKTTALRRYLAASKQGLQGIEELDYILK
                     QAEKLLKNAKLKLDLSLARGLNYYTGCIIEVKALDAEMGSIAGGGRYDNLTGVFGLPG
                     VSGVGVSFGADRIYDVLTQLTLFPATSVLTTQLLITNFGEEETAWCLPLAAELRENGF
                     RVEVYPDADKIKKQLSYADSKGIPFVLMAGADEIKQNKVTLKNMHTGQQNLMSPQDLL
                     RFLKTTTL"
     gene            1611..>2376
                     /locus_tag="PKY68_08795"
     CDS             1611..>2376
                     /locus_tag="PKY68_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOH25163.1"
                     /translation="MTATELSEQELFRRQSLQALRDMGIDPYPAEMYPTSAFSDEIKA
                     NFKDEEEPKREVCIAGRIMSRRIMGKASFMELKDSQGRIQVYVSRDEICPNEDKELYN
                     TVFKKLLDIGDFVGIKGYVFRTQMGEISVHARELKVLAKTLRPLPIVKTKDGVVYDAF
                     SDPELRYRQRCVDLVVNEWVKDIFIKRTRIFNSMRNFFNERGYLEVDTPVLQNIPGGA
                     AARPFVTHHNALDIPLYLRVANELYLKRLIVGGFEGV"
BASE COUNT          625 a          592 c          603 g          556 t
ORIGIN      
        1 atgtccagcg cctacgcggc gcgtttcacc attctgccgg tggaaatcaa tgccagctcc
       61 agggcggtga cttggctcaa caaaacgggt atcaccaacg tatcgactca tccaatggcg
      121 gttgatgcgc ttaaatgggc tgcctatacc tatgtgattg ccgctctgag ttcgttggca
      181 acgctgattt attatattct gatttttctt ggaggaagaa atcgaaacta aggagttatg
      241 gctattcaaa aaccatccat ccccaagggt acccgcgatt ttacacccga ggagacagcc
      301 aaacgcaatt atattttcaa caccatacgt tccgctttcg aaagctacgg ctttcaggcc
      361 atcgaaactc ctgccttgga gcagttgtcc acgctgatgg gcaaatacgg ggacgagggc
      421 gataaattgt tgttcaagat tttgaattcg ggcgactttg ccggcaaact cagcgaccag
      481 gaattgctga gcagagaaac cggccggctg acccggctta tttcggagaa gggactgcgt
      541 tacgacctga cagtgccttt tgcccgctac gttgtgatgc accgcaacga aattgtcttt
      601 cctttcaaac gttaccagat tcagccggtt tggcgggctg atcgtcccca aaaagggcgt
      661 taccgggaat tttttcaatg cgacgccgat gttgtgggca gcgattccct gctcaacgag
      721 gtagaactgc tgcaattgct cgatacggtg ttcaacgatt tgggcatcag ggtttgcatc
      781 aaactcaaca accgtaagat tctggccggg ctggctcaaa tactgggaca ggaagaacgt
      841 ctcacagaca ttaccgtagc catagacaaa ctcgacaaaa tcggtttgga caatgtccgt
      901 gcggaattgg tcgaaaaagg catccccgcg cagaccgtca gagaattgga gccggtgctt
      961 ttgttgcagg gcagtaacag cgaaaaaaca actgcactcc gtcggtacct ggctgcaagc
     1021 aaacaaggat tgcaaggcat cgaagaactc gactatattc tgaagcaggc tgagaagctc
     1081 ctgaaaaatg ccaagctgaa actggaccta tcgctggcca gaggcttgaa ttactatacc
     1141 ggctgcatca tcgaagtaaa agccctcgat gccgaaatgg gcagtattgc cggaggcggc
     1201 cgttacgaca acctgaccgg ggtcttcggc ctgccggggg tttctggcgt aggagtttcc
     1261 ttcggggccg accgcattta cgatgtgctg actcaactga ctcttttccc cgcaacctct
     1321 gtattgacca ctcaattgct tattaccaac ttcggagagg aagaaacggc ctggtgcctg
     1381 cctttggccg ccgaactgcg cgaaaatggt tttagagtgg aggtttatcc cgatgcggac
     1441 aaaataaaaa aacagctcag ctacgccgac agcaaaggca ttcctttcgt gctgatggcc
     1501 ggagccgacg aaataaaaca aaacaaggtt acactaaaaa atatgcatac cgggcaacag
     1561 aatttgatgt ccccgcaaga cttgctccgg tttttaaaga ccacaaccct atgacagcaa
     1621 cagaattaag cgaacaggaa ctttttcgtc gccagagctt gcaagctttg cgcgatatgg
     1681 gtatcgatcc ctatccggcc gagatgtatc caacttctgc cttttcggac gaaatcaaag
     1741 ccaacttcaa ggacgaagag gaacccaaac gtgaagtctg cattgccggg cgcattatgt
     1801 cacgccgcat tatgggcaaa gcttcgttta tggagctcaa agattcgcag ggacgtatcc
     1861 aggtttatgt gagccgcgac gagatttgtc cgaacgaaga caaagagctt tacaacactg
     1921 tgtttaaaaa actgctggac atcggcgact ttgtgggcat aaaaggttac gtatttcgca
     1981 cccagatggg agaaatttcc gttcatgccc gtgagctgaa ggtgctggcc aaaaccctgc
     2041 gccccctgcc catagtcaaa accaaagacg gggtggtgta cgatgctttc agcgatccgg
     2101 aacttcgcta ccgtcaacgc tgcgtagatc tggtggtaaa cgaatgggta aaagatattt
     2161 tcatcaaacg tacacgcatt ttcaattcca tgcgtaattt tttcaacgaa agaggttacc
     2221 tggaagtaga tactcccgtg ttgcagaaca ttcccggcgg agcagccgcc cggccttttg
     2281 tgacgcatca caatgccctg gacattccgc tctaccttag agtggccaat gaactttacc
     2341 tgaagcgatt gatagtgggt ggtttcgaag gcgttt
//
LOCUS       DAOSGL010000331         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_44120_length_2365_cov_4.311688, whole genome shotgun sequence.
ACCESSION   DAOSGL010000331 DAOSGL010000000
VERSION     DAOSGL010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44120_length_2365_cov_4.311688"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKY68_08800"
     CDS             <1..904
                     /locus_tag="PKY68_08800"
                     /inference="COORDINATES: protein motif:HMM:NF037048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOH25164.1"
                     /translation="EEPELLLLERCRHLGIPCLLLHNKSDMQALNRAVLPAIEASIPV
                     LEFSALYPENYLPALLKTIRQLLPESSTQPDSLFKGIVSRGDMVLLVTPIDRGAPKGR
                     LILPQVMAIRDLLENEAFCVVLKETQLEDYLNSSGARPALVITDSSVFDQVNKLLPPD
                     ISLSSFSIAFARLRGPFEDYIKGTPYLDKLQDGDRVLIREACTHLPSCQDIGRVKLPA
                     WIRRYTGKQLEIELCPANNKALHPMASYAMVIQCGACMSTPRQVRAGLAEAMAAGVPL
                     SNYGLAIAWMNGIFDRVISPFVKL"
     gene            916..2034
                     /gene="hydE"
                     /locus_tag="PKY68_08805"
     CDS             916..2034
                     /gene="hydE"
                     /locus_tag="PKY68_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOH25165.1"
                     /translation="MDTPVSFFTNHPPSEEELAYWLEPGSVEKDRQLFDRAYAVKIEH
                     PGPRLHLRFLLELSNVCSRDCLYCGVRRSNREIERYSLQPEVAMEVLRRAKSRGYRNI
                     VLQAGERSDKTFTRAVIRLVEQLRREGGEDFRIVLSLGEQPTATYRDWKQAGADRYLL
                     RIETSNPELYRKIHPPGSAYTLGARLRSLEALREAGFQTGTGVMIGLPFQTLRDLAAD
                     LLFFKAWEVDMVGMGPYLEHHATPLFAYRHQLLTVKERYLLSLRMIALLRCLMPDINI
                     VASTALQSVGLPDTEPLRDALMAGANVMMLNVTPESCQKAYSLYENKFIPGPDSEKYL
                     VDLARQLNTWGEEIALEDPGDPLHYLKTKNTILKKSLK"
     gene            2065..>2365
                     /locus_tag="PKY68_08810"
     CDS             2065..>2365
                     /locus_tag="PKY68_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOH25166.1"
                     /translation="MNKKVFVSGCYDMLHSGHVAFFKEAAQYGDLYVGIGSDATVFEL
                     KGRHTINSDSERLYMVKAVRYVKDAWINKGSGLMDFEEDLRQFGADIFIVNEDGHS"
BASE COUNT          553 a          585 c          639 g          588 t
ORIGIN      
        1 ggaagaacct gaactcctgt tgctggagcg ttgccgccat ctgggaattc cctgcttgct
       61 gttgcacaac aaatccgaca tgcaagctct gaaccgggct gtactgcccg ccatcgaggc
      121 tagtattccg gtgttggaat tcagtgcctt gtatccggaa aactacttgc cggcactatt
      181 aaagacaata aggcaattgc tccctgaaag ctctacgcaa ccggattcgc tgtttaaagg
      241 catagtgagc aggggcgata tggtgctgtt ggtaacaccc atcgaccggg gagcgcccaa
      301 agggcggctg atcctgcccc aggtgatggc catacgcgat ttgttggaaa acgaggcttt
      361 ttgtgtggtg ctcaaagaaa ctcagcttga ggactatttg aacagtagtg gtgcaaggcc
      421 tgccctggtt attaccgaca gcagcgtctt tgatcaggta aacaaactat tgccgcctga
      481 tatttccctg agcagtttca gcattgcctt tgcgcgattg cgaggtccct tcgaagatta
      541 tattaaggga accccctacc tggataaact acaggacgga gatcgcgtgc ttattcggga
      601 ggcctgcact catttaccca gttgccagga cataggccgg gtgaagctgc ccgcctggat
      661 acgccgctac acgggcaaac aacttgaaat cgaattgtgt ccggccaaca acaaagccct
      721 tcaccccatg gcctcttacg ctatggtgat tcagtgcgga gcatgtatga gtactccccg
      781 gcaagtcagg gccggtctgg ccgaagctat ggctgccggg gttcccctga gcaactacgg
      841 cctggccatt gcctggatga acggtatttt tgatcgggtg atcagccctt ttgttaagct
      901 ctgaatctgc ggtttatgga tactccggtt tcgtttttta ccaaccatcc tccttcggaa
      961 gaagaattgg cctattggct ggagccgggt tctgtcgaaa aagaccggca attgttcgac
     1021 cgggcctatg ctgtcaaaat cgagcatccg ggtccccgtt tgcatctgcg ttttttgctg
     1081 gaattgtcga atgtatgtag cagagattgc ctttattgcg gtgttcggcg cagcaatcga
     1141 gagattgagc gttacagtct gcagcctgaa gttgcgatgg aggttttgcg ccgggccaaa
     1201 agccggggct atcgcaacat tgttttgcag gccggagagc gcagcgataa aacctttacc
     1261 cgcgccgtta tccgtctggt ggaacagttg agacgggagg gaggggagga ttttcgaatt
     1321 gtgctctctt tgggcgagca acccaccgcc acataccgcg actggaaaca ggcaggggcc
     1381 gaccgttacc tgctgcgtat tgaaacaagc aatccggaac tataccgcaa aattcaccct
     1441 ccgggcagcg cctatacctt gggagcccga ctgcgaagtc tggaagcctt acgtgaggcc
     1501 ggtttccaga ccggcaccgg tgttatgata ggcctgcctt ttcaaacgct ccgggacctg
     1561 gcagccgatc tcctgttttt taaagcctgg gaggtagata tggtgggcat gggcccttac
     1621 ctggaacatc acgccactcc tttgtttgcc taccggcatc aactcctgac tgtaaaggag
     1681 cgttatttgc ttagtttacg catgattgcc ctgctgcgtt gcttgatgcc cgatatcaat
     1741 atagtggcgt cgacggcctt gcagtcggtg ggtctgccgg atacggaacc tttgcgcgat
     1801 gccctgatgg caggagctaa cgtaatgatg ctcaatgtta cacccgaaag ctgccaaaaa
     1861 gcctattctt tgtacgaaaa taaatttatc cccggccccg attcggagaa gtatttagtt
     1921 gatcttgccc gccaactgaa tacttggggt gaagaaatag ctttggagga tcccggcgat
     1981 ccgctgcatt atcttaaaac gaaaaacaca atactgaaaa aatcattaaa atgactatct
     2041 tgcgggccga ttagactatt accgatgaat aagaaagttt ttgtgtcggg ctgctacgat
     2101 atgttgcaca gcggccacgt ggcttttttc aaggaagctg cccaatatgg agatctttac
     2161 gtaggcatag gctcggacgc caccgttttt gaactcaaag gacgtcatac catcaattcc
     2221 gactcggagc gcctttatat ggtaaaggcc gtgcgttatg taaaagacgc ctggataaac
     2281 aagggcagcg ggctgatgga ttttgaagaa gacctgaggc aattcggtgc cgatattttt
     2341 attgtgaacg aagacgggca ttcgc
//
LOCUS       DAOSGL010000332         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_44583_length_2349_cov_4.267219, whole genome shotgun sequence.
ACCESSION   DAOSGL010000332 DAOSGL010000000
VERSION     DAOSGL010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44583_length_2349_cov_4.267219"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PKY68_08815"
     CDS             complement(<1..1174)
                     /locus_tag="PKY68_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOH25167.1"
                     /translation="MAKKKTNVRIRKKDLKSDVIRFFQENRQKKYDYLQLAGTFNLKR
                     QTQQLMLFDILEELHRQGFLDKAAGNRYSTSKQKLTNNGAHGDSYESILEEFDISEDY
                     PEKALQAAELLSDSIPGEEYAYREDFRTVCTFTIDPWDAKDFDDALSLRKLPNGNWEV
                     GVHIADVTYYVKQGSAIDREAEKRGTSIYLVDRTIPMLPERLSNGLCSLRPDEDKRCF
                     SCIFEMNEAAQVKNHRIVRTVIRSDRRLNYEEALDIIQGKNDPVQEEIRVLNELAKKL
                     QSKRFKNGAIRFESSEICFKVDEKGKPLEIHFKTPTEANNLIEEFMLLANKTVAEFIG
                     KKSKKTFVYRIHDSPDLEKLEDVARFIRRFGHNLKLQGGKKAVAASINQLLGEVQGR"
     gene            complement(1180..>2349)
                     /locus_tag="PKY68_08820"
     CDS             complement(1180..>2349)
                     /locus_tag="PKY68_08820"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HOH25168.1"
                     /translation="NRRFIRHPYFRKSILSVFEPLVPPSCQPIFFVYLEAAPDSIDVN
                     IHPTKTEVKFEHEQIIWPILNAAVRQSLGKFHAVPSLDFDKENAPEIRVFTGEKDVPP
                     PKVDFDPSYNPFQRKSDSVHKAGISFPDRDKQISHWEQLYRDFKAEGKSAVELPQTES
                     GPISKIQEEKESYLKEELDLLFDENSQIEALQTLFSASETLDEIGSNLFQVFGRYIVL
                     VTADALLLIDQHRAQTRILFEQYSNQLDTGQSFSQTLLFPEILVLDKNRLIEFESIKP
                     DLISLGFQFEKNGENYLITATPEHTQGLHPVAIVTDLLDASMESLPNFSNELKDKLAL
                     RLAKSTAIPYGQKLSLIEMKNLCQHFFDCAIRNHTPEGKKIILRVSAQEIIKRLN"
BASE COUNT          535 a          508 c          569 g          737 t
ORIGIN      
        1 tgcggccttg tacctcgccc agcaattgat tgatggaagc tgctacggcc tttttgcctc
       61 cctgcagttt taaattatgc ccgaagcgcc tgataaaacg ggctacatcc tcgagcttct
      121 ccagatcggg gctgtcgtga attctgtaga caaaggtttt tttgcttttt ttaccaatga
      181 attcggccac cgttttgttg gccaaaagca tgaattcctc gatcaggttg ttggcttcgg
      241 tgggggtctt gaaatgaatt tccaggggct tccctttttc gtccactttg aagcagattt
      301 ctgaactctc gaaacggatg gctccgtttt tgaagcgttt actttgcagt ttcttggcta
      361 attcgtttaa aacacggatt tcttcctgaa cgggatcgtt tttcccctga ataatgtcta
      421 aggcttcttc gtagttcagg cgcctgtcag agcgtattac cgtgcgtaca atgcgatgat
      481 ttttaacctg agcggcctcg ttcatttcaa agatgcagga aaaacagcgt ttatcttcat
      541 ccggtctgag cgaacatagc ccgttactca agcgttcagg cagcataggg atggtgcggt
      601 cgaccaagta gatcgaagtg cccctttttt cggcctcccg gtcgatggct gagccttgtt
      661 ttacgtagta agtgacatcg gcaatatgaa cgccgacttc ccagttgccg ttgggcaact
      721 ttctaagaga gagggcgtcg tcgaaatctt tggcatccca gggatcgatg gtaaaggtgc
      781 agactgtcct gaaatcttct ctgtacgcat actcttctcc gggaatacta tccgataata
      841 attcggcggc ctgcagcgct ttttcaggat aatcttcgct aatgtcgaat tcttcgagaa
      901 tactttcgta gctgtcccca tgtgcgccgt tgtttgtgag tttttgtttg gaagttgaat
      961 agcgattccc ggcagcttta tccaaaaaac cctgcctgtg gagttcttcc aaaatatcga
     1021 agagcatcag ttgctgggtt tgtcttttca aattaaaagt cccggccagt tgcaggtagt
     1081 cgtatttttt ttgccggttt tcctgaaaaa accgtataac atccgatttc aggtcttttt
     1141 ttctgattcg gacattggtc ttttttttgg ccattttact tagtttagtc gttttattat
     1201 ttcctgggcc gacacgcgca ggatgatttt tttgccctcg ggagtatgat tcctgatggc
     1261 gcagtcgaag aaatgctggc acaaattctt catctctatc agactcaatt tttgtccgta
     1321 gggtatggcg gtcgatttgg ccaaacgcag ggccagctta tctttcagtt cattcgaaaa
     1381 gttgggcaaa gactccatgg aggcatcgag caaatcggtg acaatggcta ccggatgtaa
     1441 accctgagta tgttcgggag tggctgttat cagatagttt tcgccgtttt tttcaaattg
     1501 aaagcccaaa ctgattaaat cgggtttgat gctctcaaat tcgatcagcc ggtttttgtc
     1561 cagtacgagg atttccggga aaagcaaggt ttgcgaaaaa gattgccccg tgtcgagttg
     1621 gttgctgtat tgttcaaaca aaatcctggt ttgggctctg tgctgatcta tcagcaaaag
     1681 tgcatctgcc gtaaccagga caatatagcg accgaaaacc tgaaagagat ttgagccgat
     1741 ttcgtccagg gtttccgagg ctgaaaacag ggtttgtaag gcctcaatct gagagttttc
     1801 atcgaaaaga aggtctaatt cctcttttag gtaagattct ttttcctcct gaattttgct
     1861 tatcgggccg gattctgttt gcggtaattc tacagcactt ttaccttccg ccttgaaatc
     1921 gcgatacaat tgttcccaat ggcttatttg tttgtccctg tccggaaatg atatccccgc
     1981 tttgtgcacg ctgtcgcttt tgcgctgaaa aggattgtag ctgggatcaa agtccacttt
     2041 gggaggaggc acgtcttttt ctccggtgaa tacccggatt tcgggagcat tttctttgtc
     2101 aaagtccagt gagggaacgg cgtgaaattt tcccagactt tgacgtacgg cggcatttag
     2161 gatgggccag atgatttgtt cgtgttcaaa ctttacttcg gtttttgtgg gatgtatgtt
     2221 cacgtcaata ctgtcgggag cagcttcaag ataaacaaaa aagatcggtt gacacgacgg
     2281 aggcaccagg ggttcaaata cgctcaaaat agattttctg aaataggggt gtcttataaa
     2341 tcgccggtt
//
LOCUS       DAOSGL010000333         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_44692_length_2346_cov_2.381493, whole genome shotgun sequence.
ACCESSION   DAOSGL010000333 DAOSGL010000000
VERSION     DAOSGL010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44692_length_2346_cov_2.381493"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKY68_08825"
     CDS             complement(<1..680)
                     /locus_tag="PKY68_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOH25169.1"
                     /translation="MNFLLLLQSVAGDTLPDLTALAEGVETSQRMNFFDMAVKGGWIM
                     IPLLLLSLVAVYIFIERSIALKKASKENPNFMERIKDYIYDGNIDSAIKLCKQADNPA
                     ARMIEKGISRIGRPMHDVQVAIENLGNLEVARMEKGLSLLAAVSGGAPMIGFLGTVLG
                     MVRAFYDMASAGSNVDITLLSSGIYTAMVTTVAGLIIGICAYFAYNYLVSQVSIIVNN
                     MEAKTMEF"
     gene            complement(689..1402)
                     /locus_tag="PKY68_08830"
     CDS             complement(689..1402)
                     /locus_tag="PKY68_08830"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOH25170.1"
                     /translation="MTKLSVNINKIALLRNSRGGNFPNVCEMALLCEKYGADGITVHP
                     RPDERHIRYRDVIDLKPLLSTEFNIEGYPSANFMDLVLKVKPHQVTLVPDAPDALTSN
                     AGWDCFKHAAFLAEVLDAFCSKGIRTSLFVETDNKQIEQAAKLGADRVELYTGPYAER
                     YHKNPDAAVKPYVQAAQAARKNGLSINAGHDLNLHNLPFFRQSIPYTEEVSIGHALIV
                     DALLMGLESSIQSYKNCLK"
     gene            1568..>2346
                     /locus_tag="PKY68_08835"
     CDS             1568..>2346
                     /locus_tag="PKY68_08835"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOH25171.1"
                     /translation="MKIVLFGKPFQAEKQDQIRFILEEIARVKVEVLLCSQYHACLKQ
                     WDLHTILPAFSLTEDNDFNADYALSIGGDGTLLNVARRVGDKGIPILGINTGRLGFLS
                     AVDYKETGAMMSELLADDVVLENHSQLCLQSPNFKLPDYPYALNEIAVLKRDISAMLH
                     IHVSLNGEYLNDYQADGLIIATSTGSTAYALSVGGPILFPENPSIVLAPVAPHSLTVR
                     TIVLSDRSVIDLRVESRSQNFLVALDGRSFPLPGNAQLRVV"
BASE COUNT          606 a          547 c          560 g          633 t
ORIGIN      
        1 atgaattcca tggttttggc ctccatatta ttgactatta tgcttacctg agacaccagg
       61 tagttatacg caaaataggc acaaatacca atgataaggc ccgccacggt ggttaccatg
      121 gctgtgtaaa taccggacga aagcagcgtg atatcgacat tgctgccagc cgaggccata
      181 tcgtaaaatg ctctgaccat acccagcacg gtacccagga aaccgatcat cggtgctccg
      241 cccgatacgg ctgccagcag agagagtcct ttttccatcc tggccacttc gaggttgccc
      301 aggttttcga tagccacctg tacatcgtgc atggggcgac ctatacggga gatgcctttt
      361 tcgatcattc tggctgcggg attatctgcc tgtttacaca acttgatggc cgaatcgatg
      421 ttgccgtcgt aaatgtagtc cttgatgcgc tccatgaaat tgggattttc tttggaagcc
      481 tttttcaggg caatgctacg ctcaataaat atgtataccg ccaccagcga aagcagtaac
      541 agggggatca taatccagcc gcccttgact gccatatcaa aaaagttcat acgttggctg
      601 gtttcgacgc cttctgccaa agcagttaaa tcgggcaaag tatctccggc gacggattga
      661 agtaagagta agaagttcat acaaataatt atttaagaca atttttataa gattgaatac
      721 tgctttccag gcccataagc agggcatcca caattaaggc atgcccaatg gaaacctctt
      781 cggtatatgg aatgctctgg cggaaaaagg gcaaattatg taaattcagg tcgtgtccgg
      841 cattgatact caagccgttt tttctggctg cttgtgcggc ttgtacgtag ggttttacag
      901 cggcatcggg gtttttatgg taacgttcgg cataggggcc ggtgtacagc tccacccggt
      961 cggcacccag tttggctgcc tgttctattt gtttattgtc ggtttctaca aagagtgaag
     1021 tccttatccc ctttgaacaa aaagcatcca gcacttcggc cagaaatgca gcgtgtttaa
     1081 agcaatccca gccggcgttg gaggtaaggg catccggagc gtccggcaca agggtcacct
     1141 gatgaggctt gactttcagc accaggtcca taaaattcgc cgaagggtag ccttcgatgt
     1201 tgaattcggt acttaacaga ggttttagat ctattacgtc ccgatagcga atatgtcttt
     1261 cgtcgggccg gggatgtacc gtaatgccat cggcaccgta tttttcgcaa agcaaggcca
     1321 tttcgcatac attggggaaa ttgcctcccc tggaattgcg taacagggct attttgttga
     1381 tgtttacact taactttgtc atatcaaata tttttcccca cttttgtgcc gtcacaaccg
     1441 gtgcggcatc aaacatagtg caaagtcaat taaaaccaca aaaataatag aataatccga
     1501 tgaatgctta agttttcgga aaaaagatta ttattgcaaa tatttaatat acttaagtta
     1561 aaaatttatg aaaattgtcc tttttggaaa accttttcag gcagaaaaac aagatcagat
     1621 cagatttatt ctggaggaga ttgcccgggt caaggtggaa gtcctgctgt gcagccaata
     1681 ccatgcctgt ctgaaacaat gggatttgca taccattctt ccggcattca gcctgacgga
     1741 ggacaatgat tttaatgccg attacgccct gagtattgga ggtgacggca ccttgctcaa
     1801 tgtagcacgc agagtgggcg acaaaggtat tccaattctg ggtatcaata ccggacgcct
     1861 gggttttttg tctgccgtcg attacaagga aacaggagca atgatgtcgg agttgctggc
     1921 agacgatgtc gttctggaaa atcacagcca gctctgcctg caaagtccca acttcaagtt
     1981 gcccgattat ccttacgcat tgaacgagat tgccgtactg aaaagggata tttcggccat
     2041 gttgcatatt catgtttctc tgaacgggga atacctgaac gattatcagg cagacggtct
     2101 gattatagct acatccaccg gctcaacggc ctatgccttg agtgtgggcg gccctatttt
     2161 gtttcccgaa aatcccagca tcgttctggc tccggtagct ccccacagcc tgacggttcg
     2221 taccatcgtg ctcagtgacc gttccgtaat agacctcagg gttgaaagcc gcagccaaaa
     2281 ctttttggtg gccttggacg gacgttcatt tcccttgccc ggaaatgccc aactacgtgt
     2341 ggtgag
//
LOCUS       DAOSGL010000334         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_44705_length_2345_cov_4.022707, whole genome shotgun sequence.
ACCESSION   DAOSGL010000334 DAOSGL010000000
VERSION     DAOSGL010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44705_length_2345_cov_4.022707"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PKY68_08840"
     CDS             <1..1476
                     /locus_tag="PKY68_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOH25172.1"
                     /translation="PWPWILMAGPGIVIVAGIVAQFGLTGLIIATAIAGVLLVLMGLL
                     RLGDAIKFMPYPIIVGFTSGIALVIFSTQIKDFLGLKALDGSALSVPSDFIEKWICYA
                     KHLGTIDPWATGIAVFTVVLIFLWKKVSKVIPGSLVAIIVSTLLVVVLKKYGVEIDTI
                     GNVYPELSGGAPLPKPSTPPITLQSIRLLLGPAFTIAMLGAIESLLSAMVADGVTGKK
                     HHSNTELIAQGLANITSSFFGGIPATGAIARTMANINNGGRTPVAGIVHAIFLLLVYL
                     FLMPLAANIPFACLAGILVVVAYNMSEWRSFKSLLKNPKSDVLVLLLTFFLTVIFDLT
                     IAISLGLLLACVLFIKRVMETSSIKVIENEIERTEDPEAIAILEKISDDIEIYEIDGP
                     FFFGVANTFGEISSEVKKHQAKVRIIRMRKVPFIDSTGLKNLHSLCENSRKHHIQIIL
                     SGVNKKVYDVLDRSGLADYIGKEFIFDHINKALDKAEKILN"
     gene            1556..2245
                     /locus_tag="PKY68_08845"
     CDS             1556..2245
                     /locus_tag="PKY68_08845"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HOH25173.1"
                     /translation="MIALARSLGDSDRKETKLSKATTFYKLGQCPDCKVISTRHWKVG
                     KLKDNNDLHLESGKRMGIKPFASNAEMEKELPGLVRRRKLKALDEKANSYRLKNLTHS
                     QPYLVPKAVDLLEEIGERFHGKLKEMNLPAYYLMVSSALRTNESQQGLGKRNINATQN
                     ESSHIYGTSFDISYKEFLPKHEKPAPEGFCRHDMMRHPLAEVLTEMSKEGRCLVVKEV
                     KQACFHITVLE"
BASE COUNT          623 a          546 c          537 g          639 t
ORIGIN      
        1 ccctggccgt ggattctgat ggcggggcca ggaattgtaa ttgttgccgg aatagttgct
       61 caattcgggc ttacaggatt gattatagct actgccatcg ccggagtttt gctggtattg
      121 atggggctgc tcagattggg agacgccatc aagttcatgc cttatcccat catagtgggt
      181 tttaccagcg gaatagccct cgttattttt tcaacccaga ttaaagattt tttgggtttg
      241 aaagctttgg atggctcagc tttatctgtg ccctccgact ttatcgaaaa atggatctgt
      301 tacgccaaac accttggaac aatcgatccc tgggctaccg gtattgctgt atttaccgtc
      361 gttctgatct ttctttggaa aaaagtcagt aaagtcattc cgggatcact ggtagctatc
      421 attgtttcga ccttgctggt tgttgtactt aaaaaatacg gagtcgaaat cgacaccatc
      481 ggaaatgttt atcctgaact gagcggtggc gctccgctac ccaaacccag cacgccgccc
      541 attaccctgc aatccatccg gttattgctg gggccggcat ttaccatagc catgttgggt
      601 gccatcgaaa gcctgctatc tgccatggtg gcggacggtg ttaccggtaa aaaacaccat
      661 tccaataccg agttgatcgc tcagggcctg gcaaatatca cgtcctcttt ttttggcggc
      721 attccagcca caggagccat tgccagaacc atggccaaca ttaacaacgg aggccgcact
      781 ccggtggccg gcattgtaca cgccatcttt ttgcttctgg tatatctgtt tttgatgcct
      841 ctggctgcga atattccatt tgcctgcctg gccgggattt tggtggtagt ggcttataat
      901 atgagcgaat ggcgctcttt taaatctctg cttaaaaacc ccaaaagcga tgtgctggta
      961 ttgttgttaa ccttcttttt aacggtaatt ttcgatctta ctatagccat ttcactggga
     1021 ttattactgg cctgcgtatt gtttatcaag cgggtgatgg aaacttcaag catcaaggta
     1081 attgaaaacg aaatcgaaag aaccgaagac cccgaagcca tcgccatatt ggaaaagatc
     1141 agcgacgata ttgaaattta cgaaattgac gggcccttct tttttggtgt ggctaacacc
     1201 tttggcgaga tcagctctga agttaaaaaa caccaggcaa aagtacgtat cattaggatg
     1261 cgcaaagttc cctttatcga ttccaccggt ttaaaaaatt tacacagttt gtgtgagaac
     1321 tccagaaagc atcacataca aattattctg agcggcgtga acaaaaaagt ctacgacgta
     1381 cttgaccgct ccggtcttgc cgactacatc ggtaaagaat ttatctttga ccacatcaat
     1441 aaagctctcg ataaggcgga aaaaatactg aactaagaac tctacaacca tgcgccgttc
     1501 taaacccctt ttcggtctct atttttttgt tatcggtttt tgtacttgtc ttgttatgat
     1561 agctttggca cgttctttag gcgattccga ccggaaagag accaagttat ccaaggcgac
     1621 caccttttac aaactgggac aatgtcctga ttgtaaggtg atttctacca gacattggaa
     1681 ggtggggaag ctcaaggata acaacgacct gcatcttgag agtggaaaac ggatgggcat
     1741 caagcccttt gcgagcaatg cggagatgga gaaagaactg cccggacttg tgaggcgtcg
     1801 caaactcaaa gctttagacg aaaaggccaa cagctaccgg ttgaaaaacc tgacacactc
     1861 acagccttat ctggtaccta aagctgtcga tctgctggag gagattggcg agcgttttca
     1921 cggcaaactt aaagaaatga atctgccggc ttattactta atggtaagct cagcattgcg
     1981 aacaaacgaa agccagcagg gtctgggcaa acgcaacatc aacgccaccc aaaacgaatc
     2041 ttctcatatc tacggaacat cctttgacat ttcgtacaaa gaattcctgc ctaaacacga
     2101 aaaacccgca ccggaaggct tttgccgtca cgatatgatg cgccaccccc tggcagaagt
     2161 gttgacagaa atgtcgaaag aaggtcgttg cctcgtagtt aaggaagtta agcaggcctg
     2221 ttttcacatc acggttttgg aataaaatcg cccctcgaga ttttttgcaa cccaaggaag
     2281 gctgattctg atttacgcca tacctttcga ttaagagcct gctcctttgg ggcaggctct
     2341 ttgat
//
LOCUS       DAOSGL010000335         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_45204_length_2329_cov_2.949428, whole genome shotgun sequence.
ACCESSION   DAOSGL010000335 DAOSGL010000000
VERSION     DAOSGL010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45204_length_2329_cov_2.949428"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PKY68_08850"
     CDS             <1..1188
                     /locus_tag="PKY68_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOH25174.1"
                     /translation="QQLCAVTGFAACSLQPNSGAAGEYTGLRVIREYLKAKGQAGRNI
                     ILIPDSAHGTNPASAAQAGFSIVQVKCDANGNVDLEDWENKARENAAGLAGCMITYPS
                     THGIFEPDVRKMCRIVHQYGGQVYMDGANMNAQVGLTSPGFIGADVCHLNLHKTFAMP
                     HGGGGPGAGPICCAAHLADFLPTESWDKTKGISVASAPYGNAMLLPITYAYICMLGTA
                     GLKRATQIAILNANYLAACLKSHFSVLYTGAEGFVAHELILDCRPFKEAGISETDIAK
                     RLMDYGFHAPTLSFPVHGTLMIEPTESESLEELDRFVQAMESIYREIEEVRTGNYPTD
                     NNLLVNSPHAEYLLTADEWPYPYPRSRAAYPLPWIEGNKFQIPVSRVDNAYGDRNLVC
                     CTY"
     gene            1307..1912
                     /locus_tag="PKY68_08855"
     CDS             1307..1912
                     /locus_tag="PKY68_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOH25175.1"
                     /translation="MNKQNKPKTEHTQEEILQKEQAGEDKSRETKVDSEQKKGKSKQQ
                     EQSKETNAKRIQELESKYNQLKESNLRLLAEYENYRKRSMREKAELIKAGGESVLIGI
                     LSVVDDFERGLAAVQDNSEITAFKEGMELIYRKLTAYLKQHGVVAIETKDQDFDTDLH
                     EAITTIPATDEKQRGKVVECIQKGYFLHDKVIRFAKVVVAN"
     gene            1949..>2329
                     /locus_tag="PKY68_08860"
     CDS             1949..>2329
                     /locus_tag="PKY68_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOH25176.1"
                     /translation="MAKRDYYEVLEVSRNATEEEIKKAYRQKAIQYHPDKNPGDKKAE
                     EMFKEAAQAYEVLSNPEKRQRYDQFGHAGVEGAAGGGFSGGGFTMEDIFSQFGDIFGG
                     HFGGSSFGGTSFGGSGFGGFGGFGG"
BASE COUNT          683 a          571 c          577 g          498 t
ORIGIN      
        1 caacaactct gtgccgtaac aggttttgca gcctgttctc tgcaacccaa ttcgggagcc
       61 gccggcgaat acaccggact aagggtgatc agggaatatc tcaaagccaa gggtcaagcc
      121 ggcagaaata tcatcctcat ccccgattcg gctcacggaa ccaatcccgc cagcgcagct
      181 caggcaggct tttccatagt tcaggttaaa tgcgatgcca acggtaacgt cgacctggaa
      241 gactgggaaa acaaagcccg cgaaaacgct gccggacttg ccggttgcat gattacttat
      301 ccttctaccc acggtatttt cgaaccggac gttcgtaaaa tgtgccgtat cgttcatcaa
      361 tacggcggac aggtttatat ggacggagcc aatatgaatg cccaggttgg cctgaccagt
      421 cccggtttta tcggagccga cgtttgccac ctgaatctgc acaaaacatt tgccatgccc
      481 cacggaggcg gtggcccggg tgcaggcccc atttgttgtg ccgcccatct ggcggatttt
      541 ctacctacag agtcctggga caaaaccaag ggtatttcgg tagcttctgc cccttacggc
      601 aacgccatgc tgctgcccat cacctatgcc tatatttgta tgttgggaac agcaggcctg
      661 aaacgggcaa cccaaatagc cattctcaat gccaattacc tggccgcttg tctgaaatcg
      721 catttctccg tgctttatac aggagccgaa ggctttgtgg ctcacgagct gattctcgac
      781 tgcagacctt tcaaagaagc cggcatcagc gaaacagata ttgccaagcg ccttatggat
      841 tacggtttcc acgcacccac cctctccttc cccgtacacg gtacgctgat gatcgaaccc
      901 accgaaagtg aaagcctcga agagttggat cgttttgtac aggccatgga aagcatttac
      961 cgggaaattg aggaagtccg taccggcaac tatccgacag acaacaactt gctggtgaat
     1021 tcgcctcatg ccgaatatct gttaacggcc gacgaatggc cttaccccta cccccggagc
     1081 cgggctgctt atcccctgcc ctggatagaa ggtaataaat ttcaaattcc cgtatcgagg
     1141 gtggacaacg cctatggtga ccgcaacttg gtatgctgca cttactgaca gattgtcaag
     1201 atcaccgaaa tacatccgtc ataactgaca aatcggccat gcaattaggt tcaggcaaga
     1261 tatttgcagt ataaacggca agcatagatt tctatttatc aagattatga ataaacaaaa
     1321 caagcccaaa accgaacata cgcaggaaga aatcctccaa aaagaacaag ccggggagga
     1381 caaatcccgg gaaaccaaag tcgattccga acaaaaaaaa ggcaaaagca aacaacaaga
     1441 acagagcaaa gaaacgaacg ctaaacgaat acaggaactc gagtcgaagt ataatcaact
     1501 gaaagaatcc aatttgcgct tgctggccga atacgaaaat taccgcaagc gcagcatgcg
     1561 cgaaaaagcc gaacttatta aagcaggcgg cgaatcggtt ttgataggga ttttgagcgt
     1621 ggtcgacgat tttgagcgtg gtttggcggc tgttcaggat aacagcgaaa taaccgcttt
     1681 caaagaaggc atggaattga tttacagaaa actgactgcc tatctgaaac aacacggtgt
     1741 ggtagccatt gagaccaaag atcaagactt cgacaccgat ttacacgagg ccattacaac
     1801 catccccgcc acagacgaaa aacaacgcgg aaaagtggtt gagtgcattc aaaagggcta
     1861 tttcctgcac gacaaagtaa tacgttttgc caaagtagtt gtggcaaact aattcatggt
     1921 gcgggcctaa gaaaacgaag attttaccat ggcgaaaaga gattattatg aggtactgga
     1981 ggtgtcgaga aacgccaccg aagaagaaat aaaaaaagcc taccgacaaa aagccattca
     2041 ataccatccc gacaaaaatc cgggcgacaa gaaagccgaa gagatgttta aagaagcggc
     2101 ccaggcatac gaggtattga gcaatcccga aaaaaggcaa cgatacgacc agttcggtca
     2161 tgccggcgtg gaaggagctg ccggtggagg tttttcgggt ggaggtttta caatggaaga
     2221 catcttctcg caattcggcg atatttttgg cggtcatttc ggcggaagca gctttggagg
     2281 gactagtttc gggggaagcg gcttcggggg ttttggtggt tttggtggc
//
LOCUS       DAOSGL010000336         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_45271_length_2326_cov_3.130779, whole genome shotgun sequence.
ACCESSION   DAOSGL010000336 DAOSGL010000000
VERSION     DAOSGL010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45271_length_2326_cov_3.130779"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PKY68_08865"
     CDS             complement(<1..1060)
                     /locus_tag="PKY68_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH25177.1"
                     /translation="MTQKSIPYGRQHITGADVQAVKEALLSDYLTQGPRIREFEENFA
                     GYIGCKYAVAVSNGTAALHLSAIALGVKPGAKVITTPITFVASANCVRYCGGEVVFAD
                     IDPLTYLLDINSVRKILESSPKGTYQGIIPVDFAGRAVHLEEFRSLADEYGLWILEDA
                     CHAPGGYFIDSGNAKQFCGNGRYADLAIFSFHPVKHIACGEGGMITTNDENLYNKLLL
                     YRTHGITKNETVFHYENSIEFATGGCSAEPAYPGWYMEMQELGYNYRLTDIQAALGNS
                     QLKRADEGLERRREIAKIYNDSFRNKTYIKGQSGLIDGHAYHLYVIEADDRLNLYNHL
                     RNNNIYSQIHYIPAHLMPY"
     gene            complement(1060..2079)
                     /gene="pseB"
                     /locus_tag="PKY68_08870"
     CDS             complement(1060..2079)
                     /gene="pseB"
                     /locus_tag="PKY68_08870"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HOH25178.1"
                     /translation="MLDLNGKSILITGGTGSLGKALTKTIFNKWPNVKRLVIYSRDEQ
                     KQFVMDREYPHSQYPAIRFFIGDVRDKERLVRALSGIDFVIHAAAMKHVHIAEYNPTE
                     CVKTNIGGAQNVIDACLATNVKSVVALSTDKACAPINLYGATKLASDKLFIAANNIKG
                     SNPIKFSVVRYGNVMGSKGSVMPFFIEIKKKGGIIPITEPNMTRFNIPIQDGVDMVLY
                     ALENAWGGEIFIPRIPSYRIMDLAEAIAPECEHKVVGMRAGEKIHEEMISASDSFHTY
                     DLGTYYTILPQQPIWNLDEYIACFNAKKVAEGFCYNSGENKEWETVDSLRAKIKEHVD
                     PDFQV"
     gene            complement(2091..>2326)
                     /locus_tag="PKY68_08875"
     CDS             complement(2091..>2326)
                     /locus_tag="PKY68_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOH25179.1"
                     /translation="NSVYQQLASQLEQAKIQVKKDTPVFTIVEPVTVPTERSKPSRVK
                     IVVIWVFLGGVVGCGLVYGKTYFLSLRKKWEEA"
BASE COUNT          601 a          556 c          460 g          709 t
ORIGIN      
        1 aataaggcat caggtgtgca ggaatataat gtatctgcga atatatattg ttgtttctaa
       61 gatggttgta caaatttaaa cgatcgtctg cttctatcac gtataaatga taagcatggc
      121 cgtcgattag tccggactgg ccctttatat aggttttatt tcgaaaagag tcattgtata
      181 tttttgcaat ttctctcctt ctttccaaac cttcgtccgc tctttttaat tgactgtttc
      241 ctaaggcagc ctggatatct gttaagcgat aattgtatcc taattcctgc atttccatat
      301 accaaccggg ataagccggt tcggcgctac atcctcccgt ggcaaattca atgctgtttt
      361 cgtagtggaa tacagtttca tttttagtta tgccatgcgt tctgtaaagg agcagtttat
      421 tataaaggtt ttcgtcgttg gtggtaatca tacccccctc gccacaagca atatgcttaa
      481 ccggatggaa agaaaaaatg gccaggtcgg cgtatcttcc attgccacaa aattgctttg
      541 cattcccgga atctatgaaa tacccgccgg gagcgtggca ggcatcttcc agtatccata
      601 aaccgtattc gtctgccagg cttctgaatt cctctaaatg tacggctcta ccggcaaaat
      661 cgacggggat aatgccctga tacgttcctt tgggcgaact ttccagaatt tttcttactg
      721 agtttatatc gagtagataa gtaagcggat cgatatcggc aaaaaccacc tcgcccccgc
      781 aataacgtac acaattggcc gatgcaacaa atgtaatggg cgtagttata accttagcgc
      841 ccggttttac acccaaggct atggcagaaa gatgcagtgc tgcggtaccg ttcgaaactg
      901 caacagcgta tttacagcct atgtagccgg caaaattctc ttcgaactct ctgatccttg
      961 gcccttgagt caggtaatcg gaaagaaggg cttccttcac ggcctgaaca tcggctccgg
     1021 taatgtgttg ccgtccgtat ggaattgatt tttgtgtcat tatacctgaa aatccggatc
     1081 tacatgctct ttgatttttg cacgtagtga gtctacggtc tcccattcct tgttttctcc
     1141 ggaattataa cagaagcctt cggctacctt tttcgcattg aaacaggcaa tatattcatc
     1201 taaattccaa atgggttgct gtggcagtat ggtatagtat gttcccaaat cgtaggtatg
     1261 aaacgaatcg gaagcgctta tcatttcttc gtgaattttt tcccccgccc tcatacctac
     1321 aaccttgtgc tcgcactcgg gagcgattgc ttcggcaaga tccattatcc ggtaagacgg
     1381 aattctcggg atgaaaattt cacctcccca ggcattttcc agagcgtaca acaccatatc
     1441 gacaccgtct tgaatgggta tattaaatct ggtcatattg ggttcggtaa ttggaataat
     1501 accacccttt ttctttattt ctataaaaaa aggcataacc gaacctttgg agcccatcac
     1561 atttccatac ctgactacgg aaaacttaat cggattactc cctttgatat tgttggcggc
     1621 aataaataat ttatcggaag ccagttttgt tgccccgtag agattgattg gagcacaggc
     1681 tttgtctgtg gaaagagcca ccacgctttt gacgtttgtc gccaaacagg catcgattac
     1741 attttgtgct cctccaatat tggtttttac acattcggtc gggttgtact ctgctatatg
     1801 aacgtgtttc atggcagccg cgtgaataac aaaatctata ccgctcaacg ctctgacaag
     1861 gcgttcctta tcgcgaacat caccaataaa aaagcggatt gcagggtatt gggaatgagg
     1921 atactcccga tccatgacaa actgtttttg ctcatcacgc gaatagatta ccaatctttt
     1981 tacattcggc catttgttga aaatagtctt agtcaaagct ttccccaatg accctgtacc
     2041 tccggtaata agtatacttt tgccgtttag atccagcatt cctttataat ttaggcttcc
     2101 tcccatttct tcctcaagct caaaaaatag gttttcccat acacaagccc gcatcccact
     2161 actcctccca aaaacaccca aatcaccact atcttcactc tgctcggttt agacctctct
     2221 gtaggaaccg ttaccggctc cacaatggta aacaccggcg tatctttctt tacctggatc
     2281 ttagcctgtt ccagttgact ggccaattgc tgatatacgc tattgg
//
LOCUS       DAOSGL010000337         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_46649_length_2277_cov_4.154365, whole genome shotgun sequence.
ACCESSION   DAOSGL010000337 DAOSGL010000000
VERSION     DAOSGL010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46649_length_2277_cov_4.154365"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PKY68_08880"
     CDS             complement(<1..810)
                     /locus_tag="PKY68_08880"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOH25180.1"
                     /translation="MKKLVFLLSSAVLLLNACTSYDAPKAKLNRIDFQTEIDGKQNDL
                     YIMKNAAGMEVCMMNYGARIVSVMVPDQKGKFGDVVLGFDNIQDYLDNSTDFGAFIGR
                     YGNRINQGKFCLDGVEYQLSVNNFGHSLHGGVKGFQYCMFDIKQLSEQSLEVSYVSED
                     GEMGFPGQLTVKAVYTLTDDNSIDIRYEAETDKPTIVNLTNHSYFNLSADPSQSITDH
                     ILMINADAFTPVDSTFMTTGEIAPVCNTPMDFRKPEVIGKRIDDFSFEQLKN"
     gene            complement(838..1617)
                     /locus_tag="PKY68_08885"
     CDS             complement(838..1617)
                     /locus_tag="PKY68_08885"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HOH25181.1"
                     /translation="MNPFSLKGKIALVTGASYGIGMSIAKAYAQAGAKIVFNDIKPEL
                     VEKGIEEYKASGFDVKGYVCDVTDEAQVNAMVAQIEKEVGVIDILVNNAGIIKRIPAV
                     DMTAAEFRQVIDVDLNGPFIVSKAVAPGMIKKKAGKIINICSMMSELGRETVAAYASA
                     KGGLKMLTRSLASEWGEFNIQVNGLGPGYIATPQTAPLRTPGHPFNEFIIAKTPAARW
                     GTPEDLQGPAVFLASSASDFVNGHVLYVDGGILAYIGKQPK"
     gene            complement(1636..>2277)
                     /gene="kduI"
                     /locus_tag="PKY68_08890"
     CDS             complement(1636..>2277)
                     /gene="kduI"
                     /locus_tag="PKY68_08890"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOH25182.1"
                     /translation="LKAPCFLHRRELGVYNVGLGEGIVKVGDNQFTLKYKEALYLGAG
                     DREVYFESKDAANPALFYFNSLVAHKTYPDKKITKENAIVAEMGSLESSNHRNINKMI
                     VNQVLPTCQLQMGMTELAPGSIWNTMPAHIHSRRMEAYFYFDLPEDHAICHFMGEPTE
                     TRHIWMKRNQAVLSPEWSIHSAAATNNYTFIWGMGGENLDYGDQDFFTFTELR"
BASE COUNT          605 a          515 c          517 g          640 t
ORIGIN      
        1 gtttttaagt tgctcaaaac taaagtcatc aatgcgtttt ccaataactt cgggcttacg
       61 aaaatccatg ggagtattgc acacgggagc gatttcaccg gtggtcataa aggtcgaatc
      121 aaccggagta aaggcatcgg cattgatcat cagaatatga tccgttatgc tttgcgaagg
      181 atcggcactc agattgaaat aagagtggtt ggtcagattg actatggtag gtttgtcggt
      241 ttccgcttcg tagcgaatgt cgatgctatt gtcgtcggta agggtataaa cggctttaac
      301 tgtgagttgg ccggggaaac ccatttctcc gtcttcggat acataggaaa cctccaggct
      361 ctgctcgctc aattgtttaa tgtcgaacat acagtattgg aaacctttaa ctccgccatg
      421 gagactgtgt ccgaaattgt ttacggataa ttggtattct acgccatcca gacagaattt
      481 accctgattg atgcgattac cgtaacggcc gatgaaagca ccaaaatcgg tagaattgtc
      541 caggtaatcc tgaatattgt caaagcccag gactacgtcg ccgaatttac ccttttgatc
      601 gggcaccatc acagaaacga tgcgagctcc gtaattcatc atacaaactt ccatacccgc
      661 ggcatttttc atgatgtaca aatcgttttg ttttccatca atctcggtct ggaagtcaat
      721 acgattgagt tttgccttgg gggcatcata gctggtgcag gcattcagca ataacaccgc
      781 tgacgacagt aaaaatacaa gctttttcat ggcttaataa ttaagattta tcgaacgtta
      841 tttgggttgt ttaccgatat aagccaaaat accgccgtcc acgtaaagaa cgtgaccatt
      901 cacgaaatcc gaagcagaag aggccaggaa aacggccggt ccctgcaaat cttcgggtgt
      961 gccccagcga gccgcgggag ttttggcaat aataaattcg ttgaaaggat gtccgggagt
     1021 ccgtagagga gctgtttgcg gagtagcaat gtagcccggt cccagtccgt tcacctgaat
     1081 gttgaattcc ccccattcgc tggccagact gcgggtcaac attttgagac cgcccttggc
     1141 cgaagcatag gcagctacgg tttcgcgtcc caattcactc atcatggaac agatattgat
     1201 gattttaccg gcttttttct ttatcatgcc gggagcaacg gctttagata cgataaaggg
     1261 gccgttcagg tcgacgtcaa tcacctgacg aaattcggca gcggtcatat cgacggccgg
     1321 aatacgctta atgataccgg cattgtttac cagtatatcg ataacgccca cttctttttc
     1381 gatttgagcc accatggcat ttacctgagc ttcgtcggta acatcacaaa cataaccctt
     1441 gacatcaaaa cctgaagctt tgtactcttc tatgcctttt tcgaccaatt cgggtttaat
     1501 atcgttgaaa acaattttgg caccggcttg tgcgtaagcc ttggcaatag acattccaat
     1561 accataggat gcaccggtta ccagtgcaat ttttcctttt aatgaaaatg gattcatata
     1621 tatgctgttt tataattaac gtaattcggt aaaagtgaag aaatcctgat ctccgtaatc
     1681 gagattttcg ccgcccatcc cccaaatgaa ggtgtaatta ttagtggcag cagctgaatg
     1741 aatagaccat tccggcgaga gtacagcctg gttgcgtttc atccaaatgt ggcgggtttc
     1801 ggtgggttct cccatgaaat gacaaatagc gtgatcttcg ggcagatcaa aataaaaata
     1861 cgcctccata cggcggctgt gaatgtgagc aggcatggtg ttccaaatac ttccgggagc
     1921 aagctcggtc attcccattt gcagttgaca ggtaggtaaa acctgattca cgatcatctt
     1981 attgatgttt ctgtggtttg aactttccaa gctgcccatt tctgcgacaa tagcgttttc
     2041 tttggtgatt tttttatccg gatatgtctt gtgagccacc aaagagttga agtaaaataa
     2101 agcaggatta gcggcgtctt tgctttcgaa gtatacttct ctgtcgccgg cgcccagata
     2161 aagagcctcc ttgtatttta aagtaaattg attgtccccc acctttacaa taccttcgcc
     2221 caggcctaca ttatagacgc ctaattcgcg tcggtgcaaa aaacagggag ctttcag
//
LOCUS       DAOSGL010000338         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_46657_length_2277_cov_3.114311, whole genome shotgun sequence.
ACCESSION   DAOSGL010000338 DAOSGL010000000
VERSION     DAOSGL010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46657_length_2277_cov_3.114311"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1957)
                     /locus_tag="PKY68_08895"
     CDS             complement(164..1957)
                     /locus_tag="PKY68_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH25183.1"
                     /translation="MKKTANSLRKKTVVSFLLPAVRTSLFALICLLPALAFAQTADTK
                     ESEGQAYDTARSFSYYFEEGLRLKALEQYGAAFEMFRHCLSLDSTSAAAHAEIAAFYR
                     AMNQTERYVAHYEKAFALDPGNDWYGLQLAGIYQQLKRYPDAIRLLEQLVAKNPDNYE
                     LYYTLSLLHTELKQYTQAIEALDRFENSVGISQEISMQKYDLYLNLNQPKPAIKELQK
                     LQKSNPREIRYLLLVGSGWMEIGKTKKARKCFLQALDMEPENGTVRLFLSDYYKAVGD
                     SLAMNEQLHLALSNPKTELESKLNIFRTLLSTCENREDSLQIERYFELLLEQHPTAYD
                     LRDLRVRWLLLTQRKDEAVGELRNVLDLNPNQLRIWQDYLGLNLEEGNQEQIRRICLE
                     ALVYFPEEVEFWYYLALTYSMEENHSEALNAYQKCLEKADPKNRTFLSSILGYMGDIY
                     HQTGDTAEAYTHYKQALEYNPDNHLVLNNYAYFLSLDESDLSKAEKMSRRSLDFQPTN
                     STYLDTYAWVFFKQEQYRLAKIYIQRAIEYSKEPNIELLEHYGDILWFNNEKEAAVEQ
                     WKKASAMEGASDILQQKANRAVYLNKSSDEQ"
     gene            complement(1963..>2277)
                     /gene="dut"
                     /locus_tag="PKY68_08900"
     CDS             complement(1963..>2277)
                     /gene="dut"
                     /locus_tag="PKY68_08900"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOH25184.1"
                     /translation="RKLIPTGLFIELPAGYEAQIRPRSGLALKKGLTVLNTPGTIDAD
                     YRGEIGVILINLSQEEVQVEDGERICQMVIASHCQATWQEVDTLEATERGAGGFGHTG
                     KK"
BASE COUNT          505 a          519 c          570 g          683 t
ORIGIN      
        1 accttggatt ccatccggtc gaaagacctg tgttgatcaa gtatctgttg aatgcgttga
       61 taagggtctg tttttttgaa aagctttttt tggggagaac agcccgtaat caagactaag
      121 gccaaggtca gcagcagaag taagaaactt ttttgttgag ttgtcattgt tcgtcggatg
      181 atttgttcag atatacggcc cggtttgctt tttgttgtaa gatgtcggaa gctccttcca
      241 tggcagacgc ttttttccac tgttccactg ccgcttcttt ttcgttgtta aaccacagaa
      301 tatcgccgta atgttccagt aattcaatgt taggttcctt ggaatactct atggcccttt
      361 ggatgtagat tttggccagg cggtattgtt cttgcttgaa aaatacccag gcgtaagtgt
      421 ccagataagt gctgttggtg ggctggaaat ccaaacttct gcgggacatt ttttcggctt
      481 ttgacaaatc gctttcgtcg agactcaaaa aataggcata attgttcagg actaaatggt
      541 tgtcgggatt gtattccagc gcctgtttat aatgtgtgta ggcttcggct gtatcgcctg
      601 tttgatgata aatatccccc atataaccca gtatgctcga caggaaagtc ctgtttttgg
      661 gatcggcttt ttccaggcat ttttgatagg cattcagcgc ttcgctgtgg ttttcttcca
      721 tgctgtaagt cagggccagg taataccaga attctacttc ttcggggaaa tacaccaatg
      781 cttccaggca aatccggcga atctgttcct gattaccttc ttcgaggttg aggcctaaat
      841 aatcctgcca gatgcgtaat tgattgggat tcaagtcgag tacattgcgc aattcaccga
      901 cggcttcgtc tttgcgttgg gtgagcagca accagcgaac ccttagatcg cgcagatcgt
      961 aggccgtggg atgttgctca agcaggagtt caaaatagcg ctcaatctgc aggctgtctt
     1021 cccggttttc gcaggtactg agcagagttc tgaagatgtt cagtttgctc tccagttcgg
     1081 tttttgggtt gcttagggca aggtggagct gttcgttcat ggccagactg tcgccgacgg
     1141 ctttgtaata atccgacaaa aacagccgca cggtgccgtt ttcgggctcc atgtcgaggg
     1201 cttggaggaa gcatttcctg gcttttttgg tttttccgat ttccatccag ccgctaccca
     1261 ccagcaaaag atatctgatc tcgcgggggt tggacttttg aagcttttgc agttctttga
     1321 tggccggttt gggttgattc aaattcaggt acaaatcgta tttctgcatg ctgatttcct
     1381 ggctgatgcc gacggaattt tcaaaacgat ccagggcctc gatggcttga gtgtattgtt
     1441 taagctcggt gtggagcagc gacagcgtat aatacaattc gtaattgtcg ggatttttgg
     1501 ctaccagttg ttccagcagt cttatggcgt ccggataacg tttcagttgc tggtaaatgc
     1561 cggccagttg caagccgtac caatcgttgc cgggatccag agcaaaagct ttttcgtaat
     1621 gagccacata acgctcggtt tgattcatag cccggtagaa agcggcaatt tctgcgtgag
     1681 ccgccgcact agtggaatcc agactgaggc aatgcctgaa catctcaaag gcggctccgt
     1741 attgttccaa cgctttcagt cgcaggcctt cctcaaaata atagctaaaa ctgcgggctg
     1801 tgtcgtaagc ttgtccctcg ctttctttag tatcggcagt ttgggcaaag gcaagtgcag
     1861 gcagcaggca aatcaatgca aacaagcttg tcctgactgc cggcagtaaa aatgaaacga
     1921 ccgtcttttt acgtagtgaa ttagccgtct ttttcatccc atttattttt tccccgtgtg
     1981 accgaaacca ccggcacctc tctccgtggc ttcgagtgtg tctacttctt gccaggtcgc
     2041 ctgacaatga gaggcaatca ccatttgaca gattctttct ccgtcttcga cctgtacttc
     2101 ctcttgcgac aaattgatca aaataacccc tatttcgccc cggtagtcgg catcaatagt
     2161 gccgggagtg ttcagtacgg tgagtccttt tttgagggcc agaccgctgc ggggcctgat
     2221 ttgagcttcg taaccggccg gcagttcaat aaaaagtccg gtaggtatca gcttgcg
//
LOCUS       DAOSGL010000339         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_47319_length_2255_cov_4.075455, whole genome shotgun sequence.
ACCESSION   DAOSGL010000339 DAOSGL010000000
VERSION     DAOSGL010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47319_length_2255_cov_4.075455"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1074
                     /gene="miaA"
                     /locus_tag="PKY68_08905"
     CDS             196..1074
                     /gene="miaA"
                     /locus_tag="PKY68_08905"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOH25185.1"
                     /translation="MQLPNECNLLVILGPTASGKTKFAVEMAGRLNAEIISADSRQVY
                     RGMDIGTGKDLSEYTLPGGQKIPYHLIDICPAGSRYNLYEYQRDFTRAWRDIRSRNKL
                     PILCGGTGLYIEAVLKGYSLPAVPENPALRASLEGKSLAELSDILAGYKNLHNTSDTD
                     TPKRALRAIEIAEYLATHPEIKSDLPPLQPYIIGLEVEREERRRRISQRLQARLDSGM
                     VEEVRNLLQSGVAADDLLYYGLEYKYLTLYVLGELSYDEMYRQLETAIHQFAKRQMTW
                     FRGMERRGLEIHWIKA"
     gene            complement(1156..1860)
                     /locus_tag="PKY68_08910"
     CDS             complement(1156..1860)
                     /locus_tag="PKY68_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOH25186.1"
                     /translation="MVKKQKSIEQGELEDLLEICQFLNPKQKEEFICNCEIHRLKKNQ
                     MLYAEGDKPEHIMCLLRGKIKITKKGVGGRNHIVRMVRPGGYFAYRASLAGTMHSTSA
                     TAFEVSDVGVFPSGLFIKLLRQNGELAFYFIKALAVDLGFSDSRQVNLTQKHIRGRLA
                     ESLLFMRNHYGLDEDGATINIYLSREDLANLSNMTTSNAIRTLSMFVSEKVITLDGRR
                     IKIIDEDKLERISRLG"
     gene            2150..>2255
                     /locus_tag="PKY68_08915"
     CDS             2150..>2255
                     /locus_tag="PKY68_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOH25187.1"
                     /translation="MEIVPAIDLIDGKCVRLSQGDYGRRKVYSDSPLEV"
BASE COUNT          603 a          553 c          562 g          537 t
ORIGIN      
        1 caggcaacgc cggcgtccag cgctgaccag ccgtggatat cgggaggctg agcggtttgg
       61 gcttgagtgg aggggagcgc tgccaaaaat ccggatatgc actgccgggc atcaatcagc
      121 cgggctgtaa cttgcagtca gtggggagcg ctcttgattt tcttgttatc tttgcccgca
      181 aaagccccca atcccatgca attgcccaac gaatgcaatc tactggtaat actcggcccc
      241 acagccagcg gcaaaaccaa atttgccgtc gaaatggccg gccggctgaa tgcggagatc
      301 atcagcgccg attcacggca ggtttaccgc ggcatggata tcggtaccgg caaagatttg
      361 tcggaatata cattgcccgg cggacagaaa attccctatc accttatcga tatttgcccc
      421 gcaggcagcc gctataattt atacgaatac cagcgcgatt ttacccgggc ctggcgggac
      481 atccgttccc ggaacaaatt acccatactc tgcggaggaa ccggcttata cattgaagcc
      541 gtactcaaag gctatagctt gcccgccgta cccgaaaacc ccgctttgag agcttcgctc
      601 gaaggaaaat ccctggccga gctcagcgac atactggccg gttacaaaaa cctgcacaac
      661 accagcgaca ccgacacccc caaacgagcc ctcagagcca tagaaattgc cgaatacctg
      721 gctactcacc ccgaaataaa atccgatttg ccccctttgc agccctatat cataggtttg
      781 gaggtggaac gcgaagaacg aaggcgtcgc atcagccaac gattgcaggc caggctcgat
      841 tccggcatgg tagaagaagt gagaaacttg cttcaaagcg gagttgcagc cgatgacctt
      901 ctttattacg gcctggaata caagtatctt acgctttatg tgttgggcga gctcagttac
      961 gacgaaatgt accggcagct cgaaacagcc atacatcaat ttgccaaaag gcagatgacc
     1021 tggttcaggg gcatggagcg gcgaggcctc gaaatccact ggataaaggc ttaaacaatt
     1081 tgagcaaaca atacggtaag gagctgcgaa aacgagcagc aaggaatagc attaaagaat
     1141 aataacggca aagaatcaac ctaagcgact gattctctcc agtttgtctt catcaatgat
     1201 tttgatgcgg cggccgtcaa gagtgatgac tttttcggat acaaacatcg acagggtacg
     1261 gatggcgttg gaggtggtca tgttggagag attggccagg tcttcgcgcg aaaggtagat
     1321 gttgatggtg gctccgtctt cgtccaggcc gtaatgattg cgcatgaaca gcagcgattc
     1381 ggccagacgt ccgcggatat gtttttgagt aagattcact tggcgggagt cggaaaagcc
     1441 caggtcgaca gccagtgctt tgataaagta aaaagccagt tcgccgtttt gcctcaacag
     1501 cttgatgaac aatccggaag gaaacacacc cacatccgaa acttcgaaag ccgtagcaga
     1561 agtggaatgc atcgttccgg ccaaagaagc cctgtaggca aaatagcctc cgggcctgac
     1621 catacggaca atatggttgc gtccgccaac gccttttttg gtgattttga tcttaccgcg
     1681 cagcagacac atgatatgct ccggcttatc gccctcggcg tacagcatct gattcttttt
     1741 aagacgatgg atttcgcagt tgcagatgaa ttcctctttt tgtttaggat tcagaaattg
     1801 acaaatttca aggagatctt ccagttcgcc ctgctctatc gatttttgtt tcttaaccat
     1861 ttcaggcaga tttttaaagc aaaacaaccg gccttgcgag ccgattatag catagtctgt
     1921 atcttttcta accttttgtg acctttcgga tgcgcaaaga taaaaattaa tatttatacg
     1981 tagaaacggc tggtacatta agaccaaaaa acgggatgat tttgcgcttt tttctaaaat
     2041 cacacaagat aaccgcgtat tttagttact tttgcaggcc gtaaagccgg cattcatttt
     2101 ccggtgttta ccccgtatgt ggaagatggt ttaattgagt tataacacaa tggaaattgt
     2161 accggccata gatcttattg atggcaaatg tgtgcgtctc agccagggtg attacgggcg
     2221 acgcaaagtt tacagcgatt ccccgctcga ggtgg
//
LOCUS       DAOSGL010000340         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_48080_length_2231_cov_4.331342, whole genome shotgun sequence.
ACCESSION   DAOSGL010000340 DAOSGL010000000
VERSION     DAOSGL010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48080_length_2231_cov_4.331342"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKY68_08920"
     CDS             <1..331
                     /locus_tag="PKY68_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HOH25188.1"
                     /translation="LRKAEELGYRAQVQEQLADHLANKELSLIKNLYEFPVVVKEAGN
                     SYSPAVIANYVYELAKEYNQFYHDYSVLYETNSDLRDFRILLSDQTAKVIRTALYLLG
                     IEVPERM"
     gene            350..1750
                     /locus_tag="PKY68_08925"
     CDS             350..1750
                     /locus_tag="PKY68_08925"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH25189.1"
                     /translation="MKIAFHGATQTVTGSKHLISLTNGKNILLDCGMFQGLGKETVEL
                     NNDFGFNPSEVSYLILSHAHIDHCGLIPKLVKDGFRGPIFGTAATLDLSEILLLNSAY
                     IQENDVRFLNKKRFLEGRRPLEPLYTVEHVKQALTQFQKLPYNSAFGIDDDIEIQLYD
                     AGHILGASTVYLRLKENNKITRIAYSGDVGRYGETILRSPQPFPQADYIIIESTYGDR
                     LHQDADPPEDYLYEQIVETCLKKKGKLIIPAFSVGRTQDLLFALNGMELDGRLPAVKY
                     YVDSPLSTAATKVVKQHPECFNRAVQKLLKIDSDPFDFKGLHYIQDKRESQSLNSSTE
                     PCVIISASGMAEAGRVKHHIANNIEKPSTTILIVGYCEKDSLGARLKEQPEEVGIFGN
                     RFKVRADIRSIDSLSAHGDYNDLCQYLSCQDADEVKQVFVVHGEEASQLAFKRRLIKK
                     GFLDVVIPTRHQEFGL"
     gene            1777..>2231
                     /locus_tag="PKY68_08930"
     CDS             1777..>2231
                     /locus_tag="PKY68_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOH25190.1"
                     /translation="MGTQSIIAVVIFCLTYAGIIFTRLPGVNVDRPSAAFTGAVAMVL
                     FGVLSSAEAVAAIDFNTIFLLLGMMIIIAGLQMDGLFDLISKITIKYARNRIMLLRII
                     VWVTGLASAFLVNDAVVLMFTPVIISICRRAGLRPEPYLIAEILAANAG"
BASE COUNT          601 a          568 c          506 g          556 t
ORIGIN      
        1 tttgagaaaa gcggaggaat tgggataccg ggcacaagtg caggaacaac tggccgacca
       61 cttagcgaac aaggaattat cccttatcaa gaacctgtat gagtttccgg tagtagtaaa
      121 agaagccggc aacagttata gcccggcagt cattgcaaat tacgtgtatg aactggccaa
      181 agagtacaat caattttatc acgattattc tgttttgtac gagaccaatt ccgatttgag
      241 agattttcgt atccttcttt cggatcaaac agccaaagta atcagaaccg ccctgtatct
      301 tctgggtatc gaagtacccg agcggatgta atcataaaac caggatgcta tgaaaatcgc
      361 ttttcacggg gcaacccaaa ccgttacagg ctccaaacac ttaatatcgc ttacgaacgg
      421 taaaaatatt ctgttggatt gcggcatgtt tcagggtctg ggcaaagaaa ctgtagaatt
      481 aaacaacgat ttcggtttca atccctctga agtcagttac ctgattcttt cgcacgcgca
      541 catagatcat tgcggcctga tccccaaact ggtcaaggat ggctttaggg gacccatttt
      601 cggaaccgct gccaccctgg atctttcgga gattctattg ctcaattccg cttatataca
      661 agaaaacgac gttcgctttt tgaacaaaaa acgttttctc gaaggccgcc ggcctttaga
      721 gcccctttac accgtagagc atgttaaaca ggccctgact cagtttcaaa aattgcccta
      781 caacagcgct tttggaatag acgacgatat agaaattcag ctttacgatg ccggacatat
      841 cctgggggcc tccacggttt atctgaggtt aaaggaaaac aacaaaatca cacgcatcgc
      901 ttacagcgga gacgtaggcc gatacggcga aaccatactc cgctctcccc agccctttcc
      961 tcaggccgac tacatcatta tagaatccac ctacggcgac cggctgcatc aggatgccga
     1021 tccgcccgaa gattatctct acgaacaaat cgtggaaacc tgcctgaaga aaaaaggcaa
     1081 attgattatt ccggccttta gtgtaggacg aacccaggat ctgctgttcg ccctgaacgg
     1141 gatggaactc gacggtcgcc tgcctgccgt taaatactat gttgacagtc ctttatcgac
     1201 tgctgccacc aaggttgtca aacaacatcc cgaatgcttc aaccgggcag tacaaaaatt
     1261 attgaaaata gacagcgatc cttttgattt taaaggcttg cactatatcc aggataaaag
     1321 ggaatcccag tcgctcaact caagcaccga accctgtgtg atcatatctg cctccggcat
     1381 ggccgaagcc ggtagagtga aacaccacat tgccaacaat atcgagaaac cgagcactac
     1441 cattctaata gtgggctatt gcgaaaaaga ctctctgggt gccagactca aggaacaacc
     1501 cgaggaagta ggcattttcg gcaatcgctt caaggtgcgc gccgacatcc gcagcataga
     1561 ttcactctcg gcgcacggcg attacaacga cctttgccag tatttatcct gccaggatgc
     1621 tgacgaagtt aaacaagtct tcgtagttca cggcgaagaa gcatcccaac tggctttcaa
     1681 acgcagactc atcaaaaaag gctttttgga tgtcgttatt ccaacccggc atcaggagtt
     1741 tggactgtaa aaattattca ggtgatctgc cgatctatgg gaactcaatc cattattgcc
     1801 gttgtcattt tttgcctcac ttacgcgggc atcattttta cccggctgcc cggcgtcaat
     1861 gttgaccgac cctctgcagc ctttaccggg gctgtggcca tggtactctt tggggttctc
     1921 agttcggccg aagccgttgc ggccatcgat ttcaatacca tctttctact gctgggcatg
     1981 atgatcatca tagccggcct gcaaatggac ggcctttttg atttaatcag taagatcacg
     2041 atcaagtatg cccgcaaccg cataatgctg ctccggatca tcgtctgggt tacggggctg
     2101 gccagcgcct ttttggtaaa cgatgccgtc gtactgatgt ttactcctgt catcattagc
     2161 atttgccgga gagcgggact caggccggag ccttatctca ttgccgaaat tttggcggca
     2221 aacgcaggca g
//
LOCUS       DAOSGL010000341         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_48238_length_2226_cov_4.223399, whole genome shotgun sequence.
ACCESSION   DAOSGL010000341 DAOSGL010000000
VERSION     DAOSGL010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48238_length_2226_cov_4.223399"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1910
                     /locus_tag="PKY68_08935"
     CDS             72..1910
                     /locus_tag="PKY68_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOH25191.1"
                     /translation="MKRREVKFSLMFRDMWQSSGKYVPRVDQLVKIAPHIVKMGCFAR
                     VETNGGGFEQVNLLFGENPNKAVREWTKPFREAGIQTHMLDRGLNGIRMSPVPADVRK
                     LFYKVKKAQGTDIARTFCGLNDLRNIAPSITYAKEAGMISQCSLCITHSPVHTVEYYT
                     NKALELIKMGADEICIKDMAGIGRPVSLGKVVENIKKAHPEIPVSYHGHAGPGFNVAS
                     ILEVCRGGCDYIDVGMEPLSWGTGHPDVITIQEMLKDAGYDVPEINMDEYMKVRTMIQ
                     EFMDDFLGLFIPERNRLMNSLLIKPGLPGGMMGSLMNDLNSNLININKYKVKHNLPEM
                     DQDELLIKLFDEVAYVWPRVGYPPLVTPFSQYVKNLALMNVMQLEKGRERWAFIADDI
                     WDMILGKAGRLPGKLAPEIIEKAKQEGREFFEGDPQDNYPDVLDDYRKLMKEKNWELG
                     QDDEELFEYAMHRAQYEAYKSGKAKTDFLEEVEKLRQASDCRELPKEPKTITVELEGE
                     QYLVTVTFDDKAPAAMVTEDNGESVAGEGRELLSPLEGNFYLTKNTQETPLKVGDYVK
                     KGDVVCYIEAMKTYNAISSEFDGRITEIVHKPGDAVSEDDILMKIK"
     gene            1917..>2226
                     /locus_tag="PKY68_08940"
     CDS             1917..>2226
                     /locus_tag="PKY68_08940"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOH25192.1"
                     /translation="MNNIFDKLYDMTAFSNIIAEPQFLIMYAIAFILLYLGIKKEYEP
                     LLLVPIAFGVLLANFPGGGMGVVQADQNGMVEVQGVMKSIWEMPLHEIANELGLMNFI
                     Y"
BASE COUNT          656 a          506 c          541 g          523 t
ORIGIN      
        1 ttattgccgc cgccgtgtcg gaggccacca aaggcaaggg tatagttaca accatagaaa
       61 agaggtagtt tatgaagaga agagaggtca aattcagctt gatgttcagg gatatgtggc
      121 aatcgtccgg aaaatatgtc cccagagttg atcaattggt aaaaatcgca ccccacatcg
      181 taaagatggg ctgctttgca cgtgttgaaa ccaacggcgg aggatttgaa caagtcaacc
      241 tgttgtttgg agaaaatccc aataaggcag ttcgcgaatg gaccaaaccc tttcgcgaag
      301 ccggcataca gactcatatg ctcgacaggg gattgaacgg catacgcatg agtccggtac
      361 ctgccgatgt ccgtaaattg ttttataaag tcaaaaaagc ccagggcacc gatatcgcca
      421 gaactttttg cggattgaac gatctccgca acatagctcc ttccattacc tatgccaaag
      481 aagccggtat gatttcacaa tgctcgctct gcataacaca ttcccctgtc cataccgtcg
      541 aatattacac caacaaggct ctggaactca tcaaaatggg tgcggacgaa atttgcatca
      601 aagacatggc cggtatagga cgtccggtat ctttgggtaa agttgtcgaa aacatcaaaa
      661 aagctcatcc tgagattccc gtatcttacc acggccatgc cggaccgggt tttaatgtgg
      721 cttccattct ggaagtttgc cgcggaggtt gcgactacat cgatgtcggc atggagcctc
      781 tttcctgggg caccggacat cccgatgtca tcaccattca ggagatgcta aaggacgccg
      841 gttacgacgt tcccgaaatc aacatggacg aatacatgaa agtccgcact atgattcagg
      901 aatttatgga cgattttttg ggcctgttta ttccggaaag aaaccgtctt atgaattcac
      961 tgcttatcaa acccggtctt cccggcggca tgatgggcag cctgatgaac gatctgaaca
     1021 gcaacctaat aaatataaac aaatacaagg tcaagcataa tttgcccgaa atggatcagg
     1081 acgaactgct tatcaagctt ttcgacgaag tggcttatgt ttggcccaga gtgggttatc
     1141 ccccattggt cactcctttc agccaatacg tcaaaaacct cgccctgatg aacgtaatgc
     1201 agctcgaaaa aggcagagaa cgttgggctt tcatagccga cgacatttgg gatatgattt
     1261 tgggcaaggc aggacgtctg cccggtaaac tggctcccga aatcatcgaa aaagccaagc
     1321 aggaaggcag ggaatttttc gagggcgatc cccaggacaa ctatcccgac gttttggacg
     1381 attatcgcaa attgatgaaa gaaaagaact gggaattggg acaggacgat gaagaattgt
     1441 ttgagtacgc catgcaccgg gctcaatacg aagcttataa gagcggcaag gcaaaaacag
     1501 atttcctgga agaagtcgaa aaattgcgtc aggccagcga ttgccgggaa ctgcccaaag
     1561 agcccaaaac catcactgtg gaactcgaag gagaacagta cctggtcacc gttacattcg
     1621 acgacaaagc tcctgcagcg atggttaccg aagacaacgg agaatcggtt gccggtgaag
     1681 gaagagaatt gctgtcgcct ttagaaggca atttctatct gaccaaaaat actcaggaaa
     1741 ctcctctcaa agtaggcgat tacgttaaaa agggtgatgt tgtatgttac atagaagcca
     1801 tgaagacata caacgctatt tcttccgaat ttgacggcag aataacggaa attgtgcaca
     1861 agcccggaga tgctgttagc gaagacgaca tcttaatgaa aatcaagtaa tgccctatga
     1921 acaatatctt cgataagctt tacgatatga ctgccttcag caatatcatt gctgaaccgc
     1981 agttcctgat tatgtacgcc attgctttta tactgctcta tctgggcatc aaaaaggaat
     2041 acgaaccctt gttgctggta cccatcgcct ttggcgtatt gctggccaac ttccccggag
     2101 gcggtatggg agtcgttcag gcggaccaaa acggtatggt agaagttcag ggtgtaatga
     2161 aaagcatctg ggaaatgccc ctgcacgaga ttgcaaacga actgggtctg atgaacttta
     2221 tttatt
//
LOCUS       DAOSGL010000342         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_48299_length_2224_cov_3.926694, whole genome shotgun sequence.
ACCESSION   DAOSGL010000342 DAOSGL010000000
VERSION     DAOSGL010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48299_length_2224_cov_3.926694"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKY68_08945"
     CDS             <1..768
                     /locus_tag="PKY68_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOH25193.1"
                     /translation="FTWKLNPVYADVYMNTFYNAVMALQSAKTGRCVYHLPLGSPRQK
                     AYLRENDFRCCNGTSIEAFSALNTGVYYRNDTALWVNLYVPSKVHWDEKKLVLEQTGH
                     FPADTCVEFSLTTAKKTDFALKLFIPSWARKAEVYVNGQKQEAKATPNSFISLHRSWK
                     NKDKIQLVFQYDFYLKTMPDDNNVFAIFYGPMMLAFDSNEELIMKGNHESILQQLSKD
                     KESNSFRLNNNGKTYRLVPLFDVEDQSYGVYATIRNY"
     gene            843..1949
                     /locus_tag="PKY68_08950"
     CDS             843..1949
                     /locus_tag="PKY68_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH25194.1"
                     /translation="MSTNKNRWFLIAAVAFALVITMLAVTFIMPEQTNRGKKAQAVCF
                     VSFPDFFNFDIPNPWPGYEEAVDYFLTQVKAENPHFVLVAGDLVNGHWWDSPKCVEHM
                     GSIYYSAWVRRMNEYGLKYYTALGDHDLGDDPWPEHKIKLIPCFERVYAEHLRMPQNG
                     PENKKGLAYYVREGDLLIVTVETFEVIDDSMRITVCGEQLEWFEQVLEDHSDAKFKIV
                     QGHVGLWGEINRRSSSALMLEGGKESEFYQLMKKHEVDAYLGGEFHDVTVLESDGIWQ
                     IIHGSSWGREIVNTQDYLVARVKGNELKLTMKRIYMDAQGEYMWNLNKDRGPRETVRI
                     NEKTLKNGPETTGTLTIRRENNQREFLDRTGYFE"
BASE COUNT          662 a          499 c          516 g          547 t
ORIGIN      
        1 tttacctgga agctcaatcc ggtttatgcc gacgtgtaca tgaacacctt ttacaacgct
       61 gtaatggcct tgcaaagcgc gaaaacggga agatgtgttt atcacttgcc cctgggttct
      121 ccccggcaaa aagcctatct cagggaaaac gatttccgct gttgcaacgg tacttcgata
      181 gaagcttttt cggccctgaa tacaggtgtt tattatcgca acgatacggc tttgtgggtt
      241 aacttgtatg tgccctcgaa agtgcattgg gacgaaaaaa agcttgttct ggaacaaaca
      301 ggccatttcc ctgcggacac ttgtgttgaa ttcagcctta ccaccgcgaa gaaaaccgac
      361 tttgctctga agcttttcat tccctcttgg gcgcgcaaag cagaagtcta cgttaacggc
      421 cagaaacagg aggcgaaagc gacgcccaat tcctttatca gcctgcatcg ttcctggaaa
      481 aacaaagaca aaatacaatt ggtcttccaa tacgactttt atcttaaaac catgccggac
      541 gacaacaatg tgttcgccat tttctatggc ccgatgatgc tggccttcga ttccaacgag
      601 gagttgatta tgaaaggcaa tcacgagtcg atactgcaac agttgtcgaa agataaagaa
      661 agcaacagtt ttcgcctgaa caacaacggt aaaacctacc ggctagttcc tttgttcgat
      721 gtggaagatc aatcctatgg ggtatatgcc actatcagga attattagta atttagagcc
      781 gcttaattta ttcctctatt ctgaattaaa atcttaatct aaaccttcta ataccaagta
      841 ccatgagcac aaacaaaaac agatggtttc tgattgcggc agtcgccttc gcgctggtga
      901 tcaccatgtt ggcggtcacg ttcattatgc cggagcaaac aaaccgggga aaaaaagcgc
      961 aagctgtatg ttttgtaagc tttcccgatt ttttcaattt cgatatcccc aatccctggc
     1021 cgggttacga agaggctgta gattactttt tgactcaggt aaaagccgag aatccccatt
     1081 ttgtgctggt agccggcgat ttggtgaacg gccactggtg ggatagcccc aagtgtgtgg
     1141 agcacatggg cagcatctat tattcggcct gggtcagacg tatgaacgaa tacggcttga
     1201 aatattatac ggccctgggc gatcacgatt tgggagacga tccctggccc gagcataaaa
     1261 tcaagctgat accctgtttc gagagagtgt atgccgaaca cttgcgcatg ccgcaaaacg
     1321 gccccgaaaa caagaaaggc ctggcttatt acgtccgtga aggcgattta ttgatcgtta
     1381 ccgtagaaac gttcgaagtg atagacgact ccatgcgcat aactgtttgt ggcgaacaac
     1441 tcgaatggtt cgaacaagtg ctcgaagacc acagcgacgc caaattcaag atcgtccagg
     1501 gccacgtagg cttatggggc gaaatcaaca gacgttcctc gagcgccctt atgttggaag
     1561 gcggcaaaga aagcgagttt taccaactga tgaaaaaaca cgaagtggac gcctacctgg
     1621 gaggagaatt tcacgacgtc accgtattgg aatccgacgg catctggcaa atcatccacg
     1681 gctcatcctg gggcagagaa attgtcaaca cccaggatta tctggtagcc cgggtaaaag
     1741 gcaatgaact taaactgacc atgaagcgca tttacatgga cgcccaggga gaatacatgt
     1801 ggaacctgaa caaagacaga ggcccccgcg aaacagtgcg cataaacgaa aagaccctga
     1861 aaaacggccc cgaaaccaca ggcaccctaa ccatccgcag agaaaacaac caaagggagt
     1921 ttttggatag gacgggatat tttgaataaa ttggggtgtt ttaaataaat tatcttccat
     1981 ttgaaataaa cttatatatt tgcattcatt agtagaatag caatacattt gtgagtagaa
     2041 cagcctttgt aagtacgatt tttgtgcaac atcgcacaac acattaaatt aaataatgat
     2101 ggaagtaaaa cgatacgcaa tcttcttgtg tattttgttg agtttgcctt tggttttgca
     2161 agcccagcgt caaagagaga aaaggctgat agaagaacaa gtagctgcta ttagaaacaa
     2221 tccg
//
LOCUS       DAOSGL010000343         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_48772_length_2210_cov_4.068677, whole genome shotgun sequence.
ACCESSION   DAOSGL010000343 DAOSGL010000000
VERSION     DAOSGL010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48772_length_2210_cov_4.068677"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKY68_08955"
     CDS             complement(<1..325)
                     /locus_tag="PKY68_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOH25195.1"
                     /translation="MKSINLFFKHKTYNDLVMFFLRKAFSVLVIVAISILAFQSCKEI
                     DQEAYYTFKGETVVSYLENNPNQFSEFSAVLKHTGLDALLATYGTFTCFIPTNDAMRA
                     YYADSS"
     gene            complement(482..1906)
                     /locus_tag="PKY68_08960"
     CDS             complement(482..1906)
                     /locus_tag="PKY68_08960"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOH25196.1"
                     /translation="MNLVSRLLSSCLLVLFFFAVSCTREYGFEHQGMLRLSTDTLSFD
                     TIFSNVGSTTARIKVYNPYKQKLLISEIGLKTRGNSGFRLSVDGLTSLSCSDLELPPR
                     DSLYLFVEITAALQGVADPVLIEDAVYFVTNGHRQEIVLQAYAQDALVWRGKRIEQDT
                     LLKAELPYLIYDSLVIDSGAVLSVDPGVRLHFHDRAAVVIYGSIKAKGELNNPIIFRG
                     DRLDKLFVDFPYEYYPGQWYGIYLDKESYGNEFDWVEIRGAYYGIIADSSSLEQRKLE
                     INNSVIFNMVYSNLYSIHSNIRVNNSLLANSGSYTLLLLGGDAEFVHCTIANYQRLVS
                     RDGPTLALANFLGEEGKASIYYPLQKADFVNSIIYGSQLDEIGLGVAEESAHAFFFRN
                     CLIRNTVELASEYAEACIYNEDPAFMSTGKDYLYNFRIDSTSAAIDKGLESVALTIPF
                     DLDGNSRTSDIAPDLGAFEWHGLE"
     gene            complement(2010..2180)
                     /locus_tag="PKY68_08965"
                     /pseudo
     CDS             complement(2010..2180)
                     /locus_tag="PKY68_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318603.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
BASE COUNT          676 a          521 c          454 g          559 t
ORIGIN      
        1 tggaagagtc ggcataataa gcacgcatgg cgtcgttggt gggaataaag caggtaaatg
       61 tgccgtaggt agccaacaaa gcatccaaac ctgtgtgttt aagtactgca ctaaattccg
      121 aaaactgatt aggattgttc tctaaataag aaacaaccgt ttcgccctta aaagtatagt
      181 aggcttcctg gtcgatttcc ttacacgatt gaaatgcaag aatcgaaatc gcaacaatta
      241 ccaaaacaga gaatgctttc cttagaaaaa acataactaa atcgttgtag gttttatgtt
      301 taaaaaaaag gttgatgctc ttcatagcgg tggtcatata aagttttctg ccttaaccat
      361 ataaaaatcg gtgcaatttt agtacttttt gccgaatctt taatcataac cgactcaaaa
      421 aaaggattat ttagcataat ataagaaaaa aactaaatcg gtttagccat atacggccga
      481 tttactctag tccgtgccat tcaaaagctc cgaggtcggg agcaatatcc gacgtccggc
      541 tgttaccgtc caggtcgaaa gggatggtaa gagcaacact ttcgaggcct ttatctatgg
      601 cagccgacgt agaatctatt ctaaaattat ataaataatc tttaccggta ctcataaaag
      661 ccggatcctc gttataaata caagcttcgg catattctga ggccagttcg acagtatttc
      721 ttatcaggca attcctgaaa aagaaagcat gagcactttc ttccgctact cccaggccta
      781 tctcatccaa ttgtgaaccg taaataatgc tgttgacaaa atcggctttt tgcaggggat
      841 agtaaatcga agctttaccc tcctctccca aaaaattggc taaggccaga gtcggaccgt
      901 cccggctgac caggcgctga taattggcaa tggtacaatg tacgaattca gcgtcgcctc
      961 ccagtaaaag caaggtatag gagccgctgt tggccagcag gctgttgttt acccgtatat
     1021 tggaatgaat actgtataag ttggaataaa ccatgttgaa aatcacggag ttgtttatct
     1081 ccaatttgcg ctgctccaaa gaagaagaat cggcgataat accgtagtag gcaccgcgga
     1141 tttccaccca atcaaactca ttgccgtaac tctctttgtc cagatagata ccataccatt
     1201 gcccgggata atattcgtag gggaaatcca caaacaactt atccaggcga tctcctctaa
     1261 aaataatcgg attattgagc tcgcctttgg ctttgatact accgtatatc accacagccg
     1321 cccggtcgtg gaaatgcagt cggacaccag ggtcaacgct caacaccgca ccggaatcga
     1381 tcaccaggct gtcgtagatc aaataaggca actcggcttt cagcaaagtg tcctgttcaa
     1441 tgcgtttgcc cctccataca agggcatcct gggcataagc ctgcaagacg atttcctgtc
     1501 gatgcccgtt tgtaacaaaa taaacggcgt cttctatcaa tacagggtcg gctactccct
     1561 gcaaagcggc cgtaatttcc acaaacaaat aaaggctgtc gcgcggcggc agttccagat
     1621 ccgaacagct caaggaggta agtccgtcga cgctcaaacg gaaaccgcta ttgcccctcg
     1681 ttttcagtcc gatttcggaa atcagcaatt tttgtttgta cggattatac accttgattc
     1741 gggctgtggt agaaccgaca ttcgaaaaaa tagtgtcaaa actgagcgta tcggtcgata
     1801 aacgcagcat accctgatgc tcaaaaccat actcccgcgt acaggaaacc gcaaaaaaga
     1861 acagcaccaa caggcaggaa gacaataaac gagacaccag gttcattcca ctctttttta
     1921 tactgagccc tctgctcaaa gcatctatac taactccttt gctcaaaaca gaactttatg
     1981 caaaaaaaac tgtagatccg agagccaagt tacatttgtt ttaagctctt taccagccaa
     2041 ttccagaaat tttctaccgc aggaatattc agtttctcgt cgggagaatg cacacctgtc
     2101 atagtgggac caaaggaaac catatccaga tcgggatatt tctgcaggaa aagtccgcat
     2161 tccaaaccgg cgtgtatagc agtgagcacc ggctcttaac ccagaacttc
//
LOCUS       DAOSGL010000344         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_48773_length_2210_cov_3.899304, whole genome shotgun sequence.
ACCESSION   DAOSGL010000344 DAOSGL010000000
VERSION     DAOSGL010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48773_length_2210_cov_3.899304"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..1680
                     /gene="murF"
                     /locus_tag="PKY68_08970"
     CDS             403..1680
                     /gene="murF"
                     /locus_tag="PKY68_08970"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOH25197.1"
                     /translation="MKHSAITTDSRNCSVGSIFFALKGERFNGNDFVPQALEAGCAYA
                     VCDENRHWNDERCLLTEDCLSTLQALAAKHRQALKHIPLIALTGTNGKTTTKELTATV
                     LEEKFNVLHTQGNLNNHIGVPLSLLKLTKEHQLAVIEMGANHPGEIRALALLADPDYG
                     LITNVGKAHLEGFGSPENILATKTELYEYIRRKEGKVFVNLDHPELIEKSQGMQRIAY
                     SSREGKAPVLVSGRPAENAFFLGVEWTDRRNGKCYLAQSRLVGGYNLENMLAAICIGL
                     YFDVPPADINRALENYQPSNNRSQWRQTENNRLIIDAYNANPGSMKAALEYFEANTSG
                     CRALILGSMKELGDYSRAEHERLIKLIHRIKPDQTLLIGKEFKDTEAPENTMLFDTTQ
                     QCADYLKTNPLKDYTILLKGSRSNKLESLLELL"
     gene            1752..>2210
                     /locus_tag="PKY68_08975"
     CDS             1752..>2210
                     /locus_tag="PKY68_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOH25198.1"
                     /translation="MKHPIFALFVALSLLACKQKDNYPYAYLYEGLEFEMPRVEVPSF
                     PNREVSIEDFGAVGDGLTLNTEAFNEAFKSLSSKGGGSLIVPSGVWLTGPIVFQSNVR
                     LHLEKAALILFTQDKSAYPVIKTSYEGLEAWRCQSPISGRNLENIAITGQG"
BASE COUNT          648 a          564 c          490 g          508 t
ORIGIN      
        1 cgggtggaac aggctcctat atcaatgata tcagcgccct cgcttatcat ctcatctact
       61 ctggattcca ggtcgtcgag gctgcttctg gcaccggcat aaaaagaatc gggcgtaata
      121 ttaatcactc ccatgacctt gggagtatcg aagcacataa gcttccctct taggcgtata
      181 gaaggagtta cagaaggatg aggacacata agacgataat tgatttgcag ggcgttttga
      241 gccggcctct acaaagctgc aaaaatacta aatatattcc gtaatcctca gcaaaatgag
      301 caaaaaaacc gtacatttga aggcctttcg tcgcaaaaat ttactcgttg aacagactta
      361 tatattttta tggtctctct tgaacaactg tatcaattat ttttgaaaca ttccgctatc
      421 accaccgata gccgaaattg ctctgtgggt tccatatttt ttgccttgaa aggagaaaga
      481 tttaacggca acgattttgt accacaagca ttggaggccg gctgtgccta cgccgtctgc
      541 gacgaaaacc ggcattggaa cgacgaacgc tgtcttttga cagaagactg cctgagtact
      601 ttacaggcgc tggccgccaa acaccggcag gcgctgaaac atattcccct gatcgcccta
      661 accggaacca acggcaaaac caccaccaaa gaacttacgg caaccgtact ggaggaaaaa
      721 ttcaatgtcc tgcatactca gggcaacctc aacaaccata tcggcgtacc cctgagcctg
      781 ctgaaactca ccaaggaaca ccaactggca gtaatcgaaa tgggagccaa tcatccggga
      841 gaaatccgcg ccttagcttt gttggccgac cccgattacg gacttatcac caacgtgggc
      901 aaggcacatc tcgaaggctt cggttccccc gaaaacatcc tggcaactaa aaccgaactc
      961 tacgaataca tccgccggaa ggaagggaaa gtctttgtca atcttgatca tccggagttg
     1021 attgaaaaat cacagggtat gcaacgcatt gcctacagca gccgggaggg aaaagctccc
     1081 gtactggtaa gcggccggcc ggctgagaat gctttctttc tgggtgtcga atggacagac
     1141 cggcgcaacg ggaagtgtta cttagcacaa agtcgcctgg ttgggggcta taacctggaa
     1201 aatatgcttg cagccatctg catcggtttg tattttgacg ttcctccggc cgacatcaat
     1261 cgggccctgg aaaattacca accttccaac aatcgttctc aatggcggca aacggaaaac
     1321 aaccgcctga tcatcgatgc ctacaatgcc aatcccggta gcatgaaagc cgctctcgag
     1381 tatttcgaag ccaacacttc gggttgcaga gcccttattt tgggcagcat gaaagaattg
     1441 ggcgactaca gccgcgcgga acacgagcgg ctgataaaac tgattcacag aatcaaaccg
     1501 gatcaaacct tacttatagg caaagaattc aaagacactg aagctcctga aaatacgatg
     1561 ttgtttgata ccacacagca atgcgccgat tacttaaaaa ctaatccact gaaagactat
     1621 accatattgc ttaaaggctc cagaagcaac aaactcgaaa gtctgctgga actgctctaa
     1681 gaatcgacca aagacagata gaaaatagaa aattattatc cattatttac tcttaactaa
     1741 aaaacaagac tatgaaacat ccgatttttg ccttattcgt ggccctgagc ttgttggcct
     1801 gcaaacaaaa agacaattat ccatacgctt acctctatga gggcctggaa tttgaaatgc
     1861 cccgggtgga agtcccttca tttcccaacc gtgaagtctc catagaggat ttcggtgctg
     1921 tgggcgacgg tctgacatta aacaccgaag ctttcaatga agctttcaaa tcgctgtctt
     1981 ccaagggcgg aggcagcctt atcgtaccat cgggtgtttg gctgaccggc cccatcgtct
     2041 tccaatcgaa cgttcgtctg cacctagaaa aagcagctct cattctgttt acccaggaca
     2101 aatcggctta tcccgtaatt aaaacaagct acgaaggcct ggaagcctgg cgctgccaat
     2161 cgcccatcag cggaagaaat ctcgagaata ttgccatcac cggccaggga
//
LOCUS       DAOSGL010000345         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_49017_length_2202_cov_4.775035, whole genome shotgun sequence.
ACCESSION   DAOSGL010000345 DAOSGL010000000
VERSION     DAOSGL010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49017_length_2202_cov_4.775035"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKY68_08980"
     CDS             <1..591
                     /locus_tag="PKY68_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOH25199.1"
                     /translation="SGNEEVRTEQDLAIEYRSFPRQTGFRQSNPSRDFVSEGGKGEFH
                     NSYIDRGKLLHKLFSRIKKQSDAPAAVRSLAAEGLIDEEEQNKLLQYTQTALEHPRAK
                     EWYSGNYRLYNECVILYEDAQHKLREKRPDRVIRQEDKMVVIDFKFGKALPKYRRQVA
                     RYMELLRQMGHKQVEGYLWYVDEQQVEQVEAGEVEL"
     gene            588..>2202
                     /locus_tag="PKY68_08985"
     CDS             588..>2202
                     /locus_tag="PKY68_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOH25200.1"
                     /translation="MKTFLQYCAEDIYNKYADSLNELCIVFPNNRARLFFVEALYKQA
                     GKAIWAPEFSSISELFGLRCPLELGDPILLNSLLFKVYQKQGLDKESFDDFYPWGNLL
                     LKDFDNIDKNLADAGQLFRNISELAAYSDDFEYLSPEQIAAIQRFFAHFETDRSELKK
                     RFIRLWNELFNIYTQFKEELTARNMAYEGMLHRWVVEHFDDKQAFEANSYLFVGFNAL
                     SGCEKFLFGKLQKAGKARFYWDYDRYYLNNPGHEAGHFIRENLKLFPNELNESCFDNW
                     KQTAKKIQFIDSSTEHAQVHYAGIWMEQNASKAKEASLSTSQDTAIVLCNENLLPGLL
                     QCVPSQIEELNVTMGYPVKQTSVYHLIRQVLFLHCDNRGKKTFRSRYVLPLLQNPYVR
                     KCSPEAAALEKELRIKNRLYPEYDELQQDDLLSLLFSPVKTAYELGELLLKLFSLLSQ
                     TIDNDDETNTETGQTDNKTNEIYLPLFQEALFKSYTQVQRLNALLKKDLLEINLQTYK
                     NLLLQVLDLSVPFSGEPLNGLQIMGMLETR"
BASE COUNT          652 a          554 c          497 g          499 t
ORIGIN      
        1 agcgggaacg aagaagtgag gactgaacag gaccttgcca ttgaatacag aagttttccc
       61 cgccaaaccg gattccggca atccaatcct tcgcgcgatt ttgtgtcgga aggcggcaaa
      121 ggcgaatttc acaacagcta catagaccgt ggcaaactcc tgcataaatt gttttcacgc
      181 atcaaaaagc agtcggatgc tccggccgcc gtacgttcgc tggcggccga gggtttgatt
      241 gacgaagaag agcagaacaa gctgctgcaa tatactcaaa cagccctgga acatccccgg
      301 gcaaaagaat ggtattcggg caattaccgc ctgtacaatg aatgcgttat tttatacgaa
      361 gacgctcagc acaagttgcg tgaaaagcgt cccgacagag ttattcgaca agaagacaaa
      421 atggtggtga tcgatttcaa gttcggcaaa gccctgccga aataccgcag gcaagtggcc
      481 cggtatatgg aactgctccg ccagatgggg cataaacagg tggaaggcta tttatggtac
      541 gtcgacgaac aacaggtgga acaagtagaa gccggggagg tagaactatg aaaacatttc
      601 tccaatattg cgccgaggac atctacaaca aatatgccga cagcctgaat gaactttgca
      661 tcgtgttccc caacaacagg gccaggctgt tttttgtaga ggccctctac aaacaagcag
      721 ggaaagccat ctgggcaccg gaattcagca gtataagcga acttttcggt ctccgctgcc
      781 cactggaact tggcgatcct attttactca acagccttct gtttaaggtg tatcaaaaac
      841 agggactcga caaagaaagt ttcgacgatt tttacccctg gggaaatctg ctgctcaagg
      901 attttgataa catcgacaaa aacctggccg acgccggaca attgtttcgc aacatcagcg
      961 aactggcggc ctacagcgat gactttgagt atttatcgcc cgaacagatt gcggccattc
     1021 agcgtttctt tgctcatttc gaaacagatc gcagcgaatt gaaaaaacgc tttattcggc
     1081 tttggaatga attattcaac atctatactc agttcaaaga ggagctgaca gcccggaata
     1141 tggcctacga aggtatgttg catcgctggg tggttgaaca tttcgacgat aaacaagctt
     1201 tcgaagccaa ctcctatctt tttgtcggct tcaacgcctt gagcggctgc gaaaagtttc
     1261 tgttcggcaa gctgcagaaa gccggtaaag cacgcttcta ctgggactac gatcgctatt
     1321 atctaaacaa tcccggacac gaagcgggcc attttatccg tgaaaacctg aaactttttc
     1381 ccaatgagct gaacgaaagt tgttttgata actggaaaca aacagcaaaa aaaattcaat
     1441 tcatagattc ttccaccgaa catgcccagg ttcattacgc cggtatctgg atggaacaaa
     1501 acgcaagcaa ggcaaaagaa gcatccttgt ctacctccca ggatacagcc atcgtccttt
     1561 gcaacgaaaa tctcctgccc ggcctgcttc agtgcgtgcc ttcacagata gaggaattaa
     1621 atgtcaccat gggttatccg gtcaaacaaa catccgtcta ccacctgatc aggcaggtgt
     1681 tgtttttaca ctgcgacaac cggggtaaaa aaacctttcg ctcccgctat gtattgcctt
     1741 tgctgcaaaa tccctatgtc cggaaatgca gccccgaagc tgcagctctc gaaaaagaac
     1801 tgcgcattaa aaaccgcctc tatccggaat acgacgaatt acaacaagat gatttgttga
     1861 gcctcctatt cagcccggta aaaacggcct acgagctggg agaactgctg ctcaagctgt
     1921 tcagcctcct gagtcaaacc atcgacaacg acgacgaaac aaatacggaa accggccaaa
     1981 cagacaacaa aaccaacgaa atctacctgc ctttgttcca ggaagctcta ttcaaaagct
     2041 atacccaagt gcaacgcctg aacgctctcc tgaaaaaaga cctgctcgaa atcaacctgc
     2101 aaacctataa aaacctcttg cttcaggtac ttgacctgag tgtgcctttt tcgggtgaac
     2161 cgctcaatgg actgcaaatt atgggaatgc tcgaaacccg tt
//
LOCUS       DAOSGL010000346         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_49103_length_2200_cov_3.168765, whole genome shotgun sequence.
ACCESSION   DAOSGL010000346 DAOSGL010000000
VERSION     DAOSGL010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49103_length_2200_cov_3.168765"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..774)
                     /locus_tag="PKY68_08990"
     CDS             complement(124..774)
                     /locus_tag="PKY68_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOH25201.1"
                     /translation="MNEKKQVDKTHYEFYRYMSKGRWASLWHQLDEVIQLKPSNVLEV
                     GPGPGAFKQMAILFGLAVETIDLDPDLKPDHVGSATALPFADASYDVVCAFQMLEHLP
                     YEITLQAFAEMVRVSRRHVVISLPDARPVWQYRFHVPKFGTHALLFPRPIMKQPEHVF
                     DGEHYWEINKQGYELARVTNDFCRVCPLVRTYRVFENPYHRLFVFKCQGKEKGSGY"
     gene            complement(778..2106)
                     /locus_tag="PKY68_08995"
     CDS             complement(778..2106)
                     /locus_tag="PKY68_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH25202.1"
                     /translation="MIDFWGNFFEMRMQQITRRPFIRNVFTVASGTAVAQAVGMAFAP
                     FITRLYGPEAFGLQGLFVSVVSLLSIIAALGYPTAIVLPKSDADALGLAKLSIAIGFL
                     TALFTTVALVFFGVDLLKLLNAEAISSFLFLIPFAMVIWVLSSVLYQWLIRKKAYQLT
                     SRFSVFTTLLLNSAKTAMGVLAPSALVLIVTNVAGSLIGTVLTFFGWKRFVKERGEEQ
                     EATAPPATFLQLATRHKDFPLLRTPQNLINAFSQNLPVLLLASYFGASASGQYTIALS
                     VLALPANLIGASVMAVFYPRITEAIHNGENARSLIVRATIGMAVIGALPYLLVMVAGP
                     FLFASIFGQEWRTAGVYAQWLSIWLFLQYINKPAVSAIPALRLQGGLLIYEFFSTVTK
                     IFALWMGFSIYGNAVVSVALFSLFGIVAYVWLILWVVYRSGKLPAENAIG"
BASE COUNT          591 a          555 c          567 g          487 t
ORIGIN      
        1 agatcaacga caggaattcc ttcaagaagt tcctggggct gtcttttgat gatgtttctc
       61 ctgatcattc gactttctct aggttcagag gaagattctc taaagatacg atgcgcagta
      121 tcttcagtac ccactccctt tttcttttcc ttgacattta aacacaaata gacggtggta
      181 aggattttca aacacccggt aggttctgac aagcgggcac accctgcaga aatcgttggt
      241 cacccgggcg agctcgtacc cctgcttgtt aatctcccag taatgttctc cgtcgaaaac
      301 atgttccggc tgtttcatga tgggccgggg aaaaagcagg gcatgtgtcc cgaatttcgg
      361 cacatggaag cggtattgcc acaccggccg tgcgtcaggg aggctgataa ccacatgccg
      421 tcgactgacc cggaccatct ccgcaaaggc ctgcagcgtg atctcgtagg gcaggtgctc
      481 cagcatttga aaggcgcaga cgacatcata gctagcatct gcaaagggca gtgccgtggc
      541 cgaaccgaca tggtcgggtt tgaggtccgg gtctagatca atcgtctcca ccgcgagtcc
      601 aaacaggatc gccatttgct tgaaggctcc cggcccgggg ccaacctcca atacatttga
      661 cggcttgagc tggatgacct catccaactg gtgccagaga ctagcccaac ggcccttgct
      721 catatatcga taaaactcat aatgagtttt atcgacttgt tttttttcgt tcatggttta
      781 tccgattgca ttttcagccg gtagcttacc gctgcggtac accacccata gtatcaacca
      841 aacataggcg acaatgccga ataaggaaaa aagagcgact gataccacag cattgccata
      901 gatcgaaaag cccatccaca gcgcaaagat ttttgtcacc gtgctgaaga attcatagat
      961 caggagcccg ccttgcaagc gtaaagctgg aatggcggag acagctggct tgtttatgta
     1021 ttgcagaaaa agccagatcg acagccactg tgcgtacacc cctgcagtac gccattcttg
     1081 gccgaaaatc gaggcgaata gaaacgggcc ggcaaccatg accagcaggt acggcaacgc
     1141 tccgatcaca gccatgccta tagttgcccg gacaatgagc gacctagcat tttcgccgtt
     1201 atgaatcgct tcggtgatgc gtggataaaa tacagccata acggaagcgc ctatcaggtt
     1261 ggccggaagt gccagaactg atagggcaat ggtatattgt ccggatgcgc tagcaccgaa
     1321 atacgaggcc agtagcagga ccggcaagtt ctgggaaaag gcattgatca ggttttgcgg
     1381 cgtccgaagt aaaggaaagt ccttatgccg tgttgcgagc tggaggaagg tggcaggtgg
     1441 cgctgttgct tcctgctctt cgccacgttc tttcacaaac cttttccatc cgaaaaaggt
     1501 caagactgtc ccgatgagag acccggcaac attggtgacg atcagcacca gggcggatgg
     1561 agcaagcaca cccatagcag tcttggccga attgagcagc aatgtagtga acactgagaa
     1621 cctagatgtg agttggtagg ctttcttgcg aataagccat tgataaagaa cgctgctgag
     1681 aacccaaatg accatggcaa aaggtataag gaacagaaaa gatgagatcg cttcagcgtt
     1741 gagtagcttg agcaaatcga ccccgaagaa taccagggcg accgtggtaa acagggcagt
     1801 aagaaagccg atagcaatgg agagctttgc caaccccagc gcatccgcat cgctcttagg
     1861 caggactatc gctgtcggat accctagagc cgcgattatg gaaagcaagc tgaccactga
     1921 tacaaaaagc ccctgcaatc cgaatgcttc cggcccgtac aggcgggtga tgaatggtgc
     1981 aaaggccatg cccaccgctt gggcaacagc cgtaccggaa gcaacggtaa aaacgttacg
     2041 gataaagggt cgcctggtaa tttgctgcat tcgcatttca aaaaaattac cccaaaaatc
     2101 gatcactcaa gaataaacca ggtctcatat tatgatttgc gtttgattca gctccgcccc
     2161 gtgagttaca ttttggtaac ggtaactctt agggtagggc
//
LOCUS       DAOSGL010000347         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_49339_length_2193_cov_4.090271, whole genome shotgun sequence.
ACCESSION   DAOSGL010000347 DAOSGL010000000
VERSION     DAOSGL010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49339_length_2193_cov_4.090271"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PKY68_09000"
     CDS             <1..661
                     /locus_tag="PKY68_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH25203.1"
                     /translation="GWCNAVSITDAPSDGASAFETWAATINRIDLYQNIVIPATGVYR
                     LTADVRTNTPTPYTQHVYAKTKFGTFESETLDSTSVITGEGWNGMENWQTLSAEFEAP
                     VGMTVRAGLSSKGFMQFDNFRLLYFGANDTTGVVGLGAVKALPTLMIYPNPASDFIVV
                     SGIENNSTVKVFNVTGQQLYVRRADQNQLSIDLNGLNQGLYILQVESEGQVINSKFIK
                     K"
     gene            941..>2193
                     /locus_tag="PKY68_09005"
     CDS             941..>2193
                     /locus_tag="PKY68_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOH25204.1"
                     /translation="MKKLFILALTLSLAAGMQAKTQKSHHGYPIDPVPFTSVKVTDAF
                     WGQRLKASREVTIPLAFSKCEETGRYENFVRAANPSSEYKVEGYSFDDTDVYKTIEGA
                     SYSMQTYPDKALDKYIDSVIVLMAAAQEPDGYLFTARTQNPEHPHEWAGSKRWEKVED
                     LSHEFYNLGHLIEGAIAHYQATGKRSLLDIAIKYADCVCREIGDGEGQTVAVPGHQIA
                     EMALAKLYLLTGEQKYLDQAKFFLDKRGYTSRRDQYSQAHLPVIEQDEAVGHAVRAAY
                     MYSGMADVAALTGDSSYTKAIDRIWENIVSKKLYITGGIGATGSGEAFGRNYELPNMS
                     AYCETCAAIGHVYMNYRLFLLHGESKYYDVLERTLYNGLISGVSLDGGRFFYPNPLES
                     MGQHQRQAWFGCACCPSNVCRFIPSV"
BASE COUNT          596 a          572 c          499 g          526 t
ORIGIN      
        1 aggctggtgc aatgccgtca gcattaccga tgcaccctcc gacggagcca gtgcatttga
       61 aacctgggca gccaccatca acaggattga tctttaccaa aacattgtta tccccgcaac
      121 cggtgtttat cgcctgacgg ccgacgtacg caccaatact ccgactcctt atacccaaca
      181 tgtatacgcc aaaaccaaat tcggcacctt tgaatccgaa accctcgatt caaccagtgt
      241 catcaccggt gaaggctgga acggcatgga gaattggcag acgttgagcg ctgaatttga
      301 agctcccgtc ggcatgacgg ttcgcgcagg attgtcttcg aaaggcttta tgcagttcga
      361 caatttcaga ctcctttatt tcggagccaa cgacaccacg ggtgttgtag gtctgggtgc
      421 agtcaaagcc ctgccgaccc tgatgatcta ccccaatccc gcaagcgatt tcatcgttgt
      481 cagcggtatc gaaaacaact ctacggttaa ggtattcaac gtaaccggtc agcaactcta
      541 cgttcgcaga gccgaccaaa accaactgag tatcgatctg aacggactta accaaggctt
      601 gtacatcctg caggttgaat ccgaaggaca agttatcaac agcaagttta taaagaagta
      661 atgtaaacaa gctctatatc ggaaaggccg ggcaaatttg cccggccttt ctttttacct
      721 gtattccggc tggattgcct ttccatcatc aacaattagc ctaaaaatcg gacaatagtt
      781 acagccttct ggattttcat tgcacttctt tacgggtgaa ttgattttct ttgtatgtgt
      841 cacttggtgt cgcggttaaa cccaacgtga aaggtgattt gccgataaat tactatcttt
      901 agactcaaca ttttaataca ttatcctata aacgacagtt atgaaaaaac tatttattct
      961 tgcactaaca cttagtctgg ctgcaggtat gcaagccaaa acacaaaaga gtcatcacgg
     1021 ttaccccatc gatccggtgc cttttacatc ggtcaaagta acggacgcct tctggggaca
     1081 aagactcaaa gccagccgtg aagtaaccat tcctctggcc tttagcaaat gcgaagagac
     1141 cggacgttac gaaaattttg tgcgggctgc caacccaagt tcagaataca aagtggaagg
     1201 ctattctttc gacgacaccg atgtgtacaa aaccatagaa ggagccagct attccatgca
     1261 aacctatccc gacaaagcgc tggacaaata catcgacagc gtcatcgttc ttatggctgc
     1321 agcccaggaa ccggacggct atttattcac ggcccgcacc cagaatcccg aacatcccca
     1381 cgaatgggcc ggatcgaaac gctgggaaaa agtcgaagat ctcagccacg aattttacaa
     1441 cctgggacac ctgatagaag gagctattgc ccactatcag gccaccggta aacgcagcct
     1501 tctggatatt gccatcaaat acgcagattg tgtttgcaga gaaatcggcg acggagaagg
     1561 ccaaactgta gcagttcccg gccatcagat cgccgaaatg gctctggcca aattatacct
     1621 gctcaccggt gaacagaaat acctcgatca ggccaagttc ttcctggaca aaaggggtta
     1681 cacttcgcgc agagaccaat acagccaggc tcacctgccg gtgattgaac aagacgaagc
     1741 tgtgggtcat gccgttcgtg cagcttatat gtattcagga atggccgatg tggctgccct
     1801 gaccggagat tcctcctaca ccaaagccat cgatcgaatc tgggaaaata ttgtaagcaa
     1861 gaaactctat atcacaggag ggatcggtgc caccggcagc ggtgaagctt ttggaaggaa
     1921 ttacgaatta cccaatatgt cggcttattg cgaaacctgt gccgccattg gccatgtgta
     1981 tatgaactac cggcttttcc tgctgcatgg cgaatcgaaa tactacgatg ttttggagcg
     2041 cactttgtac aatgggctga tttccggagt ctcactggac ggtggacggt ttttctatcc
     2101 caatcccctc gaatctatgg gccaacatca acgtcaagcc tggtttggct gcgcctgctg
     2161 cccctccaac gtttgccgtt tcatcccgtc ggt
//
LOCUS       DAOSGL010000348         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_49646_length_2183_cov_4.039944, whole genome shotgun sequence.
ACCESSION   DAOSGL010000348 DAOSGL010000000
VERSION     DAOSGL010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49646_length_2183_cov_4.039944"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PKY68_09010"
     CDS             <1..899
                     /locus_tag="PKY68_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOH25205.1"
                     /translation="LIEVAGINPDILPEITDPGVVIGPLCDALAEESGLGKVPVIAVA
                     GHDTGSAVASVPAENECFAYLSSGTWSLMGIEVREPIINEETFQMNFTNEGGVDGTTR
                     FLKNITGMWLLEQCRKEWKKTGVSYSYPTIVELASSADGFRSLVDPDDPSFANPANMV
                     KAIVDFCTATGQPVPRNHAQFIRCIFDSLALKYNYVLGKLQQVASFKIEKLHIIGGGA
                     QNRLLNQFTSNAIGLPVVAGPVEATAIGNVMMQAKALGVVGNLQEMRTLISSSIATEV
                     FLPQDATTWSLAYQKYLNIIKK"
     gene            925..2181
                     /locus_tag="PKY68_09015"
     CDS             925..2181
                     /locus_tag="PKY68_09015"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOH25206.1"
                     /translation="MVKEKLVQQAYEIARERYAALGVDVDAALNKLQKISLSLHCWQA
                     DDVSGFENPDGELTGGIQSTGNYPGKARNIDELRADIKKATSFIPGKHRLSLHAIYGD
                     FQGKFVERDQLEPKHFASWMQWAKENEMKLDFNSTSFSHPKSEALTLSSPKKEIREFW
                     IEHTKRCRAIAEAMGVYQGSPCVMNLWIHDGSKDMTVNRLEYRRILEQSLDEIFAQKY
                     NNMKDCIESKLFGIGAEYYTVGSHDFYLGYGVKNQKIVTLDTGHFHLTESIADKISCL
                     LLFTPEIMLHVSRPIRWDSDHVTLMSDDTLELAKEIVRANALDRVNIGLDYFDGSINR
                     IGAYVIGSRATQKCFLHALLEPIEILRKYESKEQGFERLALLEECKGLPWNAVWDYFC
                     LKNNVPVGEDFIPEIQQYEKEVTSKH"
BASE COUNT          587 a          517 c          520 g          559 t
ORIGIN      
        1 ctctgatcga ggtcgccggt atcaatccgg acatattgcc cgaaataacc gatccgggtg
       61 tggtgattgg tccgctctgc gatgctctgg ccgaagaaag cggattgggc aaagttccgg
      121 ttattgccgt ggccggacac gacacgggct ctgctgtggc ttcggtgccg gccgaaaacg
      181 aatgttttgc ctacctgagt tccggcacct ggtcgttgat gggtattgaa gtcagagagc
      241 ccattatcaa cgaagagact tttcaaatga acttcaccaa cgaaggcggg gtagacggta
      301 ccacccgttt cctgaaaaac atcaccggca tgtggttgct ggaacaatgt cgcaaggagt
      361 ggaaaaagac gggagtatcc tattcctatc ccactattgt ggagctggca tcatcggccg
      421 atggtttccg ttcgctggta gatcccgacg atccctcttt tgccaatccg gcaaatatgg
      481 tcaaggccat tgttgatttc tgtacagcta ccggccagcc tgtaccccgg aatcacgcac
      541 aattcattcg ttgtattttc gacagtctgg cgctcaagta caattatgtt ttgggtaaac
      601 tgcaacaagt ggccagtttc aaaatcgaaa aactgcacat catcggagga ggtgcccaaa
      661 accggctgct taaccagttt acatccaatg ccatcggact gccggtagtg gcaggtcctg
      721 tggaggctac tgctataggt aatgtgatga tgcaggctaa agcgctggga gtggtcggga
      781 acttgcagga gatgcgtaca ctgatttctt cttctattgc caccgaagtc tttctgcctc
      841 aagacgctac tacctggtcg ctggcttatc aaaagtattt gaatatcatc aaaaaataaa
      901 cattaatcct ttaaaacgaa tattatggta aaagaaaaac tcgttcaaca ggcatacgaa
      961 attgccaggg aacgttatgc agctcttggt gtcgatgtcg atgccgcttt gaataaactc
     1021 cagaaaatat ccttgtctct gcattgctgg caggccgacg atgtcagtgg atttgaaaat
     1081 cccgacggag agttgaccgg cggcattcag tctaccggaa attatcccgg caaggccagg
     1141 aatatcgatg aattgcgggc agatattaaa aaagccacct cttttatacc cggaaaacat
     1201 cgcttgagtc ttcacgccat ttacggtgat tttcagggga aatttgttga acgcgaccaa
     1261 ctggaaccca aacattttgc aagctggatg caatgggcca aagaaaatga aatgaagttg
     1321 gatttcaact ccacctcttt ctctcatccc aaaagcgaag cgctcaccct gtcgagcccc
     1381 aaaaaagaaa tccgtgaatt ctggattgaa catactaaac gttgccgggc tattgccgag
     1441 gctatgggcg tttaccaggg ttctccctgt gtaatgaatc tttggattca cgacggctca
     1501 aaagatatga ccgtcaatcg tctggaatac cgtcgcattc tggaacaatc gctcgatgaa
     1561 atttttgcac agaagtacaa caacatgaag gattgcatcg aatccaaatt atttggtatc
     1621 ggtgcggaat actatacagt gggatctcac gatttctatc tgggttatgg ggttaagaat
     1681 caaaagattg ttaccctcga taccggccac ttccacttga ccgaaagtat agccgacaag
     1741 atttcctgcc tgctgctgtt tacccccgaa atcatgttgc acgtttcccg tcccatacgc
     1801 tgggattccg accacgtgac tcttatgagc gacgacaccc tcgaattggc caaagaaatt
     1861 gtacgtgcca atgctttgga tcgtgttaat attggtcttg actattttga cggatctatc
     1921 aaccgtatcg gagcctatgt catcggtagc cgtgccactc aaaaatgctt cctgcatgct
     1981 ttgctcgaac ccatcgaaat cctgagaaaa tacgaaagta aggaacaagg tttcgaacgt
     2041 ctggctttgc tcgaagaatg caaaggcttg ccttggaacg ccgtttggga ttatttctgc
     2101 ctgaagaaca acgttcccgt gggagaagat ttcattcccg agattcagca atacgaaaaa
     2161 gaagtaactt ctaaacactg acc
//
LOCUS       DAOSGL010000349         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_49689_length_2182_cov_4.372355, whole genome shotgun sequence.
ACCESSION   DAOSGL010000349 DAOSGL010000000
VERSION     DAOSGL010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49689_length_2182_cov_4.372355"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PKY68_09020"
     CDS             complement(<1..152)
                     /locus_tag="PKY68_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOH25207.1"
                     /translation="MNIIVAGAGEVGLHLAKMLSRENHNIVVVDQKEDVLENLNSNYD
                     LMTVLGS"
     gene            complement(165..2069)
                     /gene="dxs"
                     /locus_tag="PKY68_09025"
     CDS             complement(165..2069)
                     /gene="dxs"
                     /locus_tag="PKY68_09025"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOH25208.1"
                     /translation="MMERKTSLLDEIQYPEDLKKCSVSQLTQVCEELRQFIIDALSEN
                     PGHLGASLGTVELTVALHYVLNTPNDRIVWDVGHQAYGHKILTGRKKLFYTNRKLNGI
                     SGFPNPLENEHDHFIAGHASNSISAALGMDMAAAFKKEKRVVVAVIGDGAMTGGLAFE
                     GLNNASVYPNNLLIILNDNQYAIDKNIGGVSRYLSKISTSKHYNALRWQLYLLLKKLG
                     LMNDARKNKILRFSNSIKGLLSHQRNFFEGMDIRYFGPVDGHNVNNLVRVLRTLKDMK
                     GPKILHVCTTKGKGFPAAEKDATKYHAPGRFNKQTGECKTSDKIENQPLRFQDIFGYT
                     LIELAEKNNKIVAITPAMPTGCGLTEMSYRFPDRVFDVGIAEGHAVTFSAGLAKEGMI
                     PYCNIYSSFAQRAYDNIIHDVAILKLPVVLCLDRAGLVGEDGSTHQGAFDLAFLRCIP
                     NLTIASPLDEHEFRNMMYTAQLLGDRPFIIRYPRGKGYLANWKNNMEEIPPGKGRCLI
                     KGDKLAVLSIGPIGLTAKKVVEKLNAEGFSIALYDFRFVKPLDQELIHEIGSRFKKVI
                     TIENSVVRGGFGSAVMEAFSENNYRTEVIRKGLPDRFIPHGTVEEQHQLCALDEESIR
                     LAIISHYHQN"
BASE COUNT          540 a          484 c          517 g          641 t
ORIGIN      
        1 gaccctaaaa ccgtcatcag gtcgtaattg gaattcaggt tttccaatac gtcctctttt
       61 tgatcaacta caactatgtt gtggttttct ctggacaaca ttttggccag atgtaatccg
      121 acttcgcctg ctcctgctac aataatgttc ataacagagg atatttaatt ctgatggtag
      181 tggctgataa tggccagcct gatgctttct tcgtccagtg cacagagttg atgttgttct
      241 tcaactgtgc catggggaat aaagcggtcg ggcaagccct tacgaatcac ttctgtacgg
      301 taattgtttt ctgagaatgc ttccataaca gcgctgccaa agcctcctct gacaacactg
      361 ttctcgatag taatcacttt tttaaagcgg gaacctattt cgtgtatcag ttcctgatct
      421 aagggtttaa caaaacgaaa atcgtaaagg gcaattgaaa agccttcggc attgagcttc
      481 tctacgactt tttttgcggt caggcctatg gggcctatgc tcagtacagc cagtttatcg
      541 cctttaatca ggcaacggcc tttacccggc ggaatctctt ccatgttgtt tttccaattg
      601 gcaagataac ctttccctct gggataacga attataaacg gcctgtcgcc cagcaactga
      661 gctgtgtaca tcatgttgcg aaactcgtgt tcgtccagcg gggaagctat cgttaaatta
      721 ggaatacaac gtaaaaaagc caggtcaaaa gctccctgat gagtggatcc gtcctctcct
      781 accaggccgg ctctgtcaag gcagagcacg acgggcagtt ttagaatagc cacatcgtga
      841 atgatgttgt cgtaagctct ttgggcaaag ctggaatata tgttacagta gggaatcatg
      901 ccctctttgg ccaggcctgc cgaaaaggtg acggcatgtc cctcggctat gccaacgtcg
      961 aataccctgt cggggaaacg ataactcatt tcggtcagac cgcaaccggt aggcatagcc
     1021 ggtgttatcg ccactatttt attgtttttt tcggccagct ctatcagggt gtaaccaaaa
     1081 atatcctgaa acctcagcgg ctggttttct attttgtcgg aagttttgca ttcgccggtt
     1141 tgcttattga atctacccgg tgcatggtat ttggtggcat ccttttcggc tgcggggaag
     1201 cctttgcctt tggtggtaca aacatgcagt attttgggcc ctttcatgtc ttttagggtg
     1261 cgtaacaccc tgactaagtt gttgacgtta tgcccgtcta caggcccgaa gtagcgaatg
     1321 tccataccct cgaaaaagtt gcgttggtgg ctgagtagtc ctttgatgct gttgctgaaa
     1381 cgaaggattt tgtttttgcg ggcatcgttc atcaggccta atttttttaa taacagataa
     1441 agctgccaac gcaaggcgtt gtagtgtttg gaggtgctta ttttagaaag gtaacggctg
     1501 acgccgccga tgtttttgtc gatggcatac tggttgtcgt ttaagatgat aagcaggttg
     1561 tttgggtaaa cagaggcgtt gttgaggcct tcgaaggcta aacctccggt catggcacca
     1621 tcaccgataa cggctacaac cactcttttt tcttttttga aagcggctgc catatccata
     1681 cccaaagctg ccgagatgga attggaagcg tgtccggcaa taaaatggtc gtgctcattc
     1741 tccaaaggat tgggaaaacc gctgatcccg ttaagtttgc gattggtata aaagagtttt
     1801 ttacggccgg tcaggatctt gtgcccgtaa gcctggtgcc ccacatccca aacaatccgg
     1861 tcgttggggg tattcagcac ataatgcaaa gccacggtca gttctacggt acccaggctg
     1921 gcacccaggt gtccgggatt ttcggagagt gcatcaatga taaattgtct tagttcttcg
     1981 caaacttggg ttaattgcga gactgagcac tttttgagat cctcggggta ttgtatttcg
     2041 tccaacaaag aagttttcct ttccatcata gcgacagcaa aagtaagact ttttccataa
     2101 taaatgaatt aggtaaataa attatatcaa aaaagggggg gtcattgttt ttttatacga
     2161 aaaaactctg aaacccttac aa
//
LOCUS       DAOSGL010000350         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_50083_length_2170_cov_4.320567, whole genome shotgun sequence.
ACCESSION   DAOSGL010000350 DAOSGL010000000
VERSION     DAOSGL010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50083_length_2170_cov_4.320567"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2054)
                     /locus_tag="PKY68_09030"
     CDS             complement(<1..2054)
                     /locus_tag="PKY68_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOH25209.1"
                     /translation="MIITQTEGRNIARMYRQLLQLTRGYILPEHIRKIRSIWEKAIED
                     NLLERPVPEVQPLERCVQNALVVAREIGMKRSSVISALLYDSRLIRSLELKQLERDFG
                     PSVGNIMRGLIKVNELYDRNIVFDNENFRNLLLSFAQDVRVILILLADRLNTMRMLKR
                     YPEEMQEKIAREVFSLYVPLAHRIGLYSIKTELEDLYLKFTQRAIYDEIARKLDETKR
                     SRDEYIRTFIEPVRQKLRENLDVPFEIKGRVKSIYSIWNKLRKQQISFDSIYDLFAIR
                     VILDTDREREKSDCWKVYSLITDMFQPNPARLKDWLSIPKSNGYESLHTTVLGPGGRW
                     VEVQIRSARMDEIAERGLAAHWRYKGIRSEEGLDEMMTHIREVLSNQESDSLRLMDAF
                     KLELYEKEIYVFTPKGELCKLPAGATVLDFAYSIHTDLGNKCVGAMVNGKNVPIRYPL
                     KNGDAVEIQSGSNQSPKQDWLNYVVTARARNRIKQTLKEQTQKQAEYGREILQRRMKN
                     RKLEFDEGVMTKVVRKFKYKTLTDFHAALGTEQLDLNQVIDQYVEWYQHDKTAHEAPQ
                     VTTAEKYVIEPTPQQIQQAKEDILVIDQNLKGLDFTLSKCCHPIYGDEVFGFVSVTGG
                     IKIHRVDCPNAPQMKSRFPYRIVKARWAGKSGAGKYAASLEVIGKDDIGIVTNISSLI
                     SK"
BASE COUNT          506 a          530 c          505 g          629 t
ORIGIN      
        1 tctttggaaa taagggaact gatgttggtc acgatgccga tgtcgtcttt gcctatcacc
       61 tcgagcgaag ctgcgtattt tccggccccc gattttccgg cccagcgggc cttcacgatg
      121 cggtaaggaa aacgggattt catttggggc gcattggggc aatctacccg gtgaatttta
      181 attcccccgg taacggatac aaaaccaaag acctcatctc cgtaaatcgg atggcaacat
      241 ttggacagag taaagtccag acctttcagg ttttgatcaa ttaccagaat atcttctttt
      301 gcctgctgta tttgctgagg cgtgggttca atgacgtatt tttcggctgt ggtaacctgt
      361 ggagcttcgt gagcagtttt gtcgtgctga taccattcca cgtattgatc aatcacttga
      421 ttcaggtcga gttgttccgt tcctaaagca gcgtgaaaat cggtcagggt tttgtattta
      481 aattttcgca ccactttggt catcaccccc tcgtcaaatt ccagtttgcg attcttcatg
      541 cgccgctgta aaatttccct cccgtactca gcctgtttct gggtttgttc cttcagggtt
      601 tgcttaatgc gattgcgtgc tctggccgtt accacataat tcagccaatc ttgtttgggg
      661 ctttggttgg aaccgctctg gatttcgacg gcatcgccgt ttttgagcgg ataacgtatg
      721 ggcacgtttt tgccgttgac catggctccc acacacttgt tacccaagtc tgtgtggatg
      781 ctgtaagcaa aatcgagtac cgtagctcct gccggcaatt tgcacagttc gcccttagga
      841 gtaaatacgt aaatttcctt ttcgtacaat tccagcttaa aggcatccat caagcgcagc
      901 gaatcgcttt cctgattact gagtacttcg cggatatggg tcatcatttc gtccagacct
      961 tcttcgctgc gtatgccctt gtatctccaa tgagccgcca ggcctctttc ggcaatttcg
     1021 tccattctgg cgctgcgaat ctgcacttcg acccaacggc caccgggccc cagcacggtg
     1081 gtatgcagcg attcataacc attgctttta ggaatggata accagtcttt caacctcgcc
     1141 ggattaggct gaaacatatc ggtgatcagg gaataaacct tccaacaatc cgacttttca
     1201 cgctcccggt cggtatccaa tattaccctg atggcaaaaa gatcgtatat actatcgaaa
     1261 gaaatctgct gcttacgcag tttgttccag atggaataga tggatttcac ccggcctttg
     1321 atctcaaagg gaacatcgag gttctctctg agtttttgcc ggaccggctc tatgaaagtt
     1381 cggatgtatt cgtccctgga tcgcttggtt tcgtccagtt tgcgggcaat ttcgtcgtat
     1441 atggcccgct gggtaaattt caggtataaa tcctccagtt ctgttttgat ggaatagaga
     1501 ccaatgcggt gagccaaggg gacgtagagc gaaaacactt cccgggcaat tttttcctgc
     1561 atttcctcgg gataacgctt cagcatccgc atggtattca gacgatcggc cagaaggatc
     1621 agtattaccc ggacatcctg ggcaaacgag agtaaaagat tgcgaaagtt ttcgttatca
     1681 aaaacaatgt tgcggtcgta aagttcgttc accttgatca gccctcgcat aatattgccc
     1741 accgaggggc caaaatcccg ttcgagttgt ttaagctcca gacttcgtat cagcctggag
     1801 tcgtataaca gggcgctgat gaccgacgaa cgcttcatcc cgatttcgcg tgccaccacc
     1861 agggcatttt gaacacagcg ttccaaaggt tgcacttcgg gtacgggacg ttccagcaga
     1921 ttatcctcta tggctttttc ccagatggag cgtattttcc gaatatgttc gggaagtatg
     1981 tatccccgtg tcagttgcaa caattgacgg tacattcgtg caatgttccg gccttcggtc
     2041 tgagtgatga tcatgagtac aaactaaaaa agtcaggtaa aaatacattt tttctaccaa
     2101 aataggctat atttggcctg attatttgca acctacaatt atgagacata tcgccattac
     2161 aggagatttg
//
LOCUS       DAOSGL010000351         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_50258_length_2165_cov_4.194787, whole genome shotgun sequence.
ACCESSION   DAOSGL010000351 DAOSGL010000000
VERSION     DAOSGL010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50258_length_2165_cov_4.194787"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..>2165
                     /locus_tag="PKY68_09035"
     CDS             338..>2165
                     /locus_tag="PKY68_09035"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH25210.1"
                     /translation="MKTQFLLFVFVFISLLSSAQLKQNFRGTITDQTSKFPLPGANIS
                     LTNGDESFGTTSNASGVFEAKNLPLGRYQLTVSFMGYETFLQKNIDLESGKEKVMNVE
                     MIEKVENLDEIIVSASGKGEVRNEMAALSARSFSVAETEKYAGSWGDPSRMASNFAGV
                     ITANDSRNDIIIRGNSSLGLLWKLDGVSIPNPNHFGALGTTGGPVSMLNNNVLTNSDF
                     FTGAFPAEYGNALSGVFDLKMRNGNNQKHEFVGQVGFSGFELGAEGPLSSNKNASYII
                     NYRYSVLDVMSKLGFDIGSGVPEYQDLTMKLYLPTKKMGTFSFYAIGGKSRIIFEDTG
                     EEGGTSYDTGNDTRTVNRSEMGVVALSHRFFPDSKSNIFTSVSLSGQGVDTEIDSTGN
                     DLPDIRYYGEGNDEVRTTVSTRYTRKFDSRNSLKAGASFESYAIDYADSVDGREYDQP
                     FGVYVKGINTHESGLNLLQSFAEFQHKLNQRLTLYGGAHFQHFFYNSTNAIDPRLSIT
                     YQLLPSSKVALAYGMHSQIQPLYIYFVEDYDKNTGTYTQTNNDLEFSKAQHIVASYDQ
                     MVCRNMKLKIETYYQHLTSIPVDKVTSPFSMVNEGNKFHQQRA"
BASE COUNT          637 a          502 c          448 g          578 t
ORIGIN      
        1 gaaattactg ttataaatgc tactgaggtt tgaccacaaa aaataaggtt ttctctaaat
       61 gatgagaact taatctttgc ctacagattg ctctgtcttt ttgcttcgtg catcagaaat
      121 taaccctcaa gcaagaacag gagcatttca gaaaaaaaat aagcggtaca atccctttaa
      181 atcctctgtc ctgttcccat tcatctggcg gctgatacat atcactccaa aaatcctaca
      241 attcatccac tatgtttttt taccccattt ttttcgcctc acctttagac catgaaactt
      301 ttttattgtc attactaaac agaatattga taaaaccatg aaaacacaat ttttactctt
      361 tgtttttgta ttcatttctc tcctctcctc cgcacaattg aaacaaaatt tccgcggaac
      421 aattaccgac cagacatcaa aatttccatt gcccggagcc aatatttcgc ttacaaatgg
      481 cgatgaatcg tttggaacaa cctctaacgc atcgggtgtc ttcgaagcaa aaaacctccc
      541 tcttgggcgt tatcagctaa cggtcagttt tatgggctat gaaactttct tacaaaagaa
      601 tatcgactta gagtcgggta aagaaaaggt catgaacgtt gaaatgatag aaaaggttga
      661 aaatctcgac gaaattattg tatcggcatc ggggaaaggc gaagtccgca acgaaatggc
      721 cgcccttagt gcccggtcgt tttcggtcgc cgaaactgaa aaatatgccg gaagctgggg
      781 cgatccttca cgaatggctt ccaattttgc cggggtgatc accgcgaacg acagtcgtaa
      841 tgacataatt atcaggggaa actcatcgct tggtttgctc tggaaactcg atggggtaag
      901 cattccaaac ccaaaccatt ttggagcgtt gggaaccact ggcggtccag taagcatgct
      961 gaacaacaac gtcctgacga actccgattt tttcaccgga gcctttcctg cagaatacgg
     1021 aaatgcctta tcaggagtat tcgatttgaa aatgcgtaac ggaaataacc aaaaacacga
     1081 gtttgtcgga caagttggtt ttagtggttt cgaattaggt gcagaaggcc ctctgtcgtc
     1141 gaacaaaaat gcctcataca taattaacta ccgctattct gtactcgatg taatgagtaa
     1201 gcttggcttc gacattggca gtggggtgcc cgaatatcag gaccttacca tgaagctcta
     1261 tctgcctaca aagaagatgg gtactttttc cttttatgcc attggcggta aaagcaggat
     1321 catttttgaa gataccggcg aagagggtgg cacaagttac gataccggta acgacacccg
     1381 cacagtgaac cgctctgaaa tgggcgtagt tgcgctgtcg caccgcttct ttcccgactc
     1441 aaaatcaaac attttcacct ctgtttcatt gtcgggacaa ggagtcgaca ctgagattga
     1501 ctccaccgga aacgaccttc ccgacatccg gtattatggc gaaggaaacg acgaagtcag
     1561 aaccaccgtt tcaacccgtt atacccggaa gtttgattcg cgcaattcat taaaagccgg
     1621 ggcttcgttc gaatcgtatg ctattgatta tgccgacagt gtcgacggcc gggaatacga
     1681 tcaacccttt ggtgtatacg tgaaaggcat aaacacgcat gaaagcgggc taaacttact
     1741 tcagtcgttt gccgaatttc aacataaatt aaaccagaga cttacgcttt acggaggggc
     1801 tcacttccag cacttttttt acaactcgac caatgccatt gacccgcgct taagcattac
     1861 ctaccagctt ttgccctcgt caaaggtggc gctggcttat ggaatgcatt cgcaaattca
     1921 gcctctatac atctattttg ttgaagatta cgacaaaaat acaggcacct atacacagac
     1981 caacaatgat ttagaattta gcaaggcaca acacattgta gccagctacg accagatggt
     2041 gtgccggaat atgaaactaa aaattgagac gtattaccag catctgacct ctattcctgt
     2101 agataaagtg accagcccgt tttcaatggt aaacgaggga aacaagttcc atcagcaacg
     2161 ggctg
//
LOCUS       DAOSGL010000352         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_51075_length_2142_cov_3.951126, whole genome shotgun sequence.
ACCESSION   DAOSGL010000352 DAOSGL010000000
VERSION     DAOSGL010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51075_length_2142_cov_3.951126"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PKY68_09040"
     CDS             <1..1008
                     /locus_tag="PKY68_09040"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOH25211.1"
                     /translation="VYKFRMTNESGAVAEVCNYGATLVNVKVPDKTNKLVSVVKGFPS
                     VDGYLADRTFGTYLGATCGRYANRIAKARFVLEGREYTLAANNDDNSLHGGIEAFHTK
                     YWDFEVDEEYVHFSCESQEGEEGFPGKLKVRTSYGWSATNELSILYSATTDKATPLNL
                     TSHAYFNLAGGGNILTHRMKFHADRYIPILPDAIPTGEIRLVKDTAFDFSEFKAIGKD
                     IDKQESQLINGAGYDHCFVINKEEYGSLALAVEVFAPETGCGLKVWTSKPGVQFYSGN
                     YLRSELPASDGKPLAYREAFCLEPEFFPDSPNQSGFPDCILQAGQTYNHSTIFQFYVE
                     D"
     gene            complement(1194..1373)
                     /locus_tag="PKY68_09045"
     CDS             complement(1194..1373)
                     /locus_tag="PKY68_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25212.1"
                     /translation="MKKSIALVLLFAALFLISCQQKGGSKVDTTVVELQDSTVQVTPD
                     SCAQVAVADSLSKAE"
     gene            1635..>2142
                     /locus_tag="PKY68_09050"
     CDS             1635..>2142
                     /locus_tag="PKY68_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25213.1"
                     /translation="MSINYSCFAGENGTKFGYRGFSGGMMPHSGYFWGGKTTVRDAQG
                     AVLAVEEMRGIPLGLGGAIRFHFGDHFRVGAEGYGTTLRYGNYNSNVSIGWGGLLAAY
                     YCEYGKFKPFAGATLGGGSVKNLTLLQDNPLDDIAESAVSYRNYPFMAFCPFTGAEYC
                     LNKKIHLIF"
BASE COUNT          574 a          497 c          515 g          556 t
ORIGIN      
        1 gtgtacaaat tccgcatgac caacgagtcg ggagcggtgg ccgaagtttg taactacgga
       61 gccactttgg tcaatgtgaa agtgcccgac aaaacaaaca agcttgtcag cgtagttaag
      121 ggttttcctt cggtggacgg ttatctggcc gacagaacct tcggcaccta tctgggggct
      181 acttgtggcc gctatgccaa ccgtattgcc aaagcccgtt ttgtgctcga aggccgcgaa
      241 tacactctgg ctgccaataa cgacgacaat agcctgcacg gcggcatcga agcatttcat
      301 accaaatact gggatttcga agtcgacgag gaatacgtgc atttcagttg tgaatcgcag
      361 gagggagagg aaggctttcc cggtaaactc aaggtcagaa cctcctacgg ttggtcggcc
      421 accaacgagt tgagcatttt gtattcggcc acgaccgaca aggctacacc gctcaatttg
      481 accagccacg cctatttcaa tctggccgga ggcggcaata ttcttaccca ccggatgaag
      541 tttcacgcag atcgctatat tcccatactt cccgatgcca ttcccacggg cgaaatacgc
      601 ttggtcaaag atactgcctt cgatttcagt gagttcaaag ccatcggaaa agacatcgac
      661 aaacaggaaa gtcagttgat caacggcgcc ggctacgatc attgtttcgt aatcaacaaa
      721 gaagaatacg gcagtctggc tttggccgta gaggtgtttg ctcccgaaac cggctgcggc
      781 cttaaggtct ggaccagcaa accgggtgtt cagttttaca gcggtaatta cctgcgtagt
      841 gaactgccgg cttcggacgg aaagcccctg gcttaccgtg aggccttttg cctggaaccg
      901 gaatttttcc ccgacagccc caaccaaagc ggtttccccg attgtatcct gcaagccggt
      961 cagacctaca accacagcac tatttttcag ttttacgtag aagattgaag gagcaccctc
     1021 cccgcataat ggaacggtgc gcagttgcac cagttgggcc acccccggag aacatataaa
     1081 acaattgctt gaagatatca ggcgttttaa ggctgatacc tcaagcaatt tatctgttca
     1141 gtaagcccgc agggcttgtc gcgtggatcg atacaaaaaa aggactgata gtcttattct
     1201 gccttggaca ggctgtcggc gacggcaacc tgagcgcagg agtcaggtgt tacctgaaca
     1261 gtgctgtcct gaagttcgac aactgtagtg tcaactttag aaccaccttt ttgctgacaa
     1321 gagatcagaa aaagagcagc aaacaaaaga accaaagcaa ttgatttttt cattgaagta
     1381 attaataaaa aataagtaaa aaaggatatt gaaaaccgcc gcgaagatac gcacaatatt
     1441 ctcaaattgt tcttttttat aaaaattttt ttctctgaag aaaattattt ttataaactg
     1501 ttctgcagct tgctttttta gtatttccgg gaatgcttac ctttgcgttt ttttaaaaga
     1561 gaccggcctt tatactttta agttcggcta ttgatacaat gattcgaaga ctatttatac
     1621 cggctgtttt tttcttgagt attaactata gttgctttgc cggcgaaaac ggaacaaaat
     1681 tcggataccg gggtttttcc ggtggtatga tgcctcacag cgggtatttt tggggcggaa
     1741 agacaacagt tcgtgatgcc cagggagcag ttctcgctgt ggaagaaatg agaggaatac
     1801 ccctgggctt gggcggagcc attcgctttc atttcggaga tcattttcgg gttggcgccg
     1861 agggatacgg cactacgctc cgttacggaa attacaacag caatgtatcc ataggctggg
     1921 gaggtctttt ggccgcttac tattgcgaat acggtaaatt caaacctttt gccggagcaa
     1981 ccctgggagg tggttcggtc aaaaatctga ctttattgca ggacaatcct ctcgatgata
     2041 ttgcagaatc ggccgtatcc tacagaaact atccttttat ggctttctgt ccgtttacag
     2101 gagcagaata ctgcctgaac aaaaaaatac atctgatctt ca
//
LOCUS       DAOSGL010000353         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_51352_length_2135_cov_4.062981, whole genome shotgun sequence.
ACCESSION   DAOSGL010000353 DAOSGL010000000
VERSION     DAOSGL010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51352_length_2135_cov_4.062981"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="PKY68_09055"
     CDS             <1..1522
                     /locus_tag="PKY68_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOH25214.1"
                     /translation="DFKNILMLSVNEGQMPKNRAENSFIPYNLRRAFNLSGPEHQDAI
                     SAYYFFRALQRAENITLVYNSSTEGLNRGEMSRFMLQLAIESKLPIEILHLSSEIKTL
                     PGSPILVPKTPALMQQLYDMCDQAAANGNTQKRYLSPSMLNTYLDCRLKYYFQYLRGL
                     KKEEEISEQMDPLCFGTIFHAAAQYLYVKLILQKNGRKHSHENILASMEDIRAFNLAL
                     KAPDSPAGFLNRNAATGERLNEKIETDKQFKERIETGEQLNGRIEAADLQALYADKAC
                     LERAVEHFMAKEFFRLKDGQSLPALNGEQLIQRRLLVQFLKFLLKLDQNYAPFELLGL
                     ETFVEKHMELHPNPGRKLKLRVGGIIDRMDKKDETIRICDYKTGGKAESASFDKGLFK
                     SDQKRNRHVFQTFLYAGIVKDQYPASMVKPELVYIHQAGKPDYSSDIVVKKSENSGAK
                     TNVNNVVNDFGEFKETFETELQNVIGELFDPAVSFDQTTVFKTCEHCDFNTICRRK"
     gene            complement(1602..1958)
                     /locus_tag="PKY68_09060"
     CDS             complement(1602..1958)
                     /locus_tag="PKY68_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HOH25215.1"
                     /translation="MAKNKPQNLFDLIPSVNERIRIETGEDKLLQLVFPRFRSAWLNR
                     RLTPKGRSPYIHIKLEEHGSAVVQLIDGQRSVREIALALAEHFEQEPGYEHRIAVFIE
                     HLRRDNYISLRQPEVL"
     gene            complement(1945..>2135)
                     /locus_tag="PKY68_09065"
     CDS             complement(1945..>2135)
                     /locus_tag="PKY68_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25216.1"
                     /translation="MIAGEALMGLIIAIFALFNVFFSEMLPIEQPSYWIGLVLMFAMA
                     YLMIVLPLKKAAKTNGQK"
BASE COUNT          646 a          540 c          473 g          476 t
ORIGIN      
        1 cgatttcaaa aacatactga tgctctcggt gaacgaaggg caaatgccca aaaacagagc
       61 cgagaattcg tttattccct acaatctgcg cagggcgttc aatctgagcg gcccggagca
      121 tcaggacgcc atttccgcct attatttttt cagagcgctg caaagggccg aaaacataac
      181 gctggtgtat aacagctcca ccgaaggcct gaaccggggc gaaatgagcc gctttatgct
      241 gcaattggca atagaaagca agttgcccat cgaaatcctg catttgagtt cggaaatcaa
      301 aaccctcccg ggctctccca ttctggtccc caagaccccg gcccttatgc agcagcttta
      361 cgatatgtgc gaccaggccg cagcaaacgg gaacacccaa aagcgctatc tctccccaag
      421 catgttgaat acctacctcg actgccgttt aaaatactac tttcaatacc tgagggggtt
      481 gaaaaaagaa gaagagatca gcgaacaaat ggatcccctg tgtttcggta ctatttttca
      541 tgcggccgct caatacctct atgtaaaact aattctacaa aaaaacggac gtaagcacag
      601 ccacgaaaac attttggcaa gcatggaaga tatccgggct ttcaatctcg ctttgaaagc
      661 gccggattcc ccggccgggt ttttaaaccg taatgcagcg accggtgagc ggctcaatga
      721 gaaaatcgag acagataaac agttcaagga gagaatcgag accggtgaac aactcaatgg
      781 aaggatcgaa gccgccgacc tgcaagcctt atacgccgac aaagcctgcc tggaacgtgc
      841 agtagaacat tttatggcca aggaattttt ccgtctcaaa gacggacaaa gcctgccggc
      901 actaaatggc gaacaactta ttcaacgtag attgctggtt cagttcctga aattcttact
      961 caaactggat caaaactacg cacccttcga attgttgggc ctggaaactt tcgtagaaaa
     1021 acacatggaa ctccacccca accccggcag gaaactaaaa ctgcgagtgg gaggcatcat
     1081 cgaccgcatg gataaaaaag acgagactat ccgcatctgc gattacaaaa ccggcggcaa
     1141 agccgaaagc gcttccttcg acaagggctt gttcaaatcc gaccaaaaac gcaacaggca
     1201 cgtctttcag acctttcttt acgccggcat cgttaaggat caatatcccg cttcgatggt
     1261 taaacccgaa ctcgtatata ttcatcaggc cggcaagccc gattacagct cagatatagt
     1321 agtaaaaaaa tccgaaaatt ccggggctaa aaccaacgtc aacaatgtgg tgaatgattt
     1381 cggagaattc aaggaaactt tcgaaaccga actgcaaaac gtcatcggcg aactgttcga
     1441 tcccgcagta agcttcgatc aaacaacagt attcaaaaca tgcgagcatt gtgatttcaa
     1501 tactatttgc agacgcaagt aatatcctaa gctaagcaaa agcagactag cggttcaaaa
     1561 aaagacaata aaagggccct tttccccggc aacaggcaaa actaaagaac ctccggctgc
     1621 ctgaggctaa tgtagttatc tctacgtaga tgctcaataa aaacagctat ccggtgctcg
     1681 tagcccggtt cctgttcgaa atgctccgcc agagccagag caatctctct gacactgcgt
     1741 tgaccatcta tcaattgtac aacggccgat ccgtgctctt cgagcttaat gtgaatatag
     1801 ggcgaccttc ccttaggagt gagacgacga ttcaaccagg ctgatctgaa acgggggaag
     1861 accagctgca aaagcttgtc ttcccctgtt tctatgcgta tcctctcgtt tacagagggt
     1921 atcaggtcga ataaattttg gggtttattt ttggccattg gttttggcgg cttttttcag
     1981 aggcaatact atcatcagat aggccatcgc gaacatcagc actaagccga tccaataact
     2041 gggttgctct atgggcaaca tctccgagaa gaaaacattg aaaagggcga aaatggcaat
     2101 aatcaatccc atcaaggctt cgccggctat catac
//
LOCUS       DAOSGL010000354         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_52481_length_2103_cov_4.021484, whole genome shotgun sequence.
ACCESSION   DAOSGL010000354 DAOSGL010000000
VERSION     DAOSGL010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52481_length_2103_cov_4.021484"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKY68_09070"
     CDS             <1..246
                     /locus_tag="PKY68_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOH25217.1"
                     /translation="RIKIRYMLRRKGLAEADIQEALEQIPDNEYLDLLKALINEKQKH
                     YAGLEAYEKKAKLFRFVYSKGFEPDLINQCLDLSTDY"
     gene            219..905
                     /locus_tag="PKY68_09075"
     CDS             219..905
                     /locus_tag="PKY68_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOH25218.1"
                     /translation="MPGFINGLLNLLYPPLCVVCGRNLIADEEHLCSACLYALPRTRF
                     HSEYKNPVCERFWGKLPFEKATAAFHYQKESHMQLLMESFKYKSNRQLAFYLGSYAGI
                     QLSKQGFFEQIDLILPVPLHKNKERRRGYNQSELIARGLAHCSGLPVDTGSLGRAVEN
                     PTQTRRSIWERSRNVQQIFRLSSFEKLAGKHVLLVDDVLTSGSTMEACGNALLEAPGL
                     RLSFFALALA"
     gene            1017..2039
                     /locus_tag="PKY68_09080"
     CDS             1017..2039
                     /locus_tag="PKY68_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOH25219.1"
                     /translation="MKKKTILTTFLIFGLLVHSRAEDIVKSGFNLGALPAISYNSDLG
                     FQYGVILNLFHYGDGSRYPAFDHSMYLEVSRYTKGTGVYRLYYDSDRLIKGVRSFIDL
                     SYMTNLMQDFFGYNGFRSVYSVDSVAANSAFYKMHQNKFHLMADFQGNLPMDNLKWLL
                     GYGLYNYSIAPVNLEKLNKGKSPEEQIPDRPGLYQKYVEWGLISPGEKHGGMLNLLKA
                     GLMYDSRNAKTNPDKGIFTEALLEMAAPLLNESPYVNYIVMHRQYQSLIKNRLNAAIR
                     IGVQGKIGNKAAPFYRKPQLISTFATRTVTTGLGGNSTLRGILQNRVVGDAFCAGQRG
                     ITLEND"
BASE COUNT          560 a          454 c          498 g          591 t
ORIGIN      
        1 aggattaaaa tccgctacat gctgaggaga aagggccttg ccgaagccga cattcaggaa
       61 gccttggagc aaatacccga caatgaatac ctcgacttgc tgaaagcttt gatcaacgaa
      121 aaacaaaaac attatgccgg tttggaggct tacgaaaaaa aagccaagct ctttaggttt
      181 gtctacagta aaggctttga acccgatctt ataaaccaat gcctggattt atcaacggat
      241 tattaaatct gctttatccc cctctctgcg ttgtgtgtgg cagaaacctt attgccgacg
      301 aagaacatct ttgttcggct tgcctttatg ctttgccgcg cacacgtttc cattccgagt
      361 ataaaaatcc tgtctgtgaa cgtttttggg ggaaactgcc tttcgaaaag gctacggcag
      421 cctttcatta tcaaaaggaa tcgcatatgc agttgttgat ggagagtttt aagtataaat
      481 ccaaccggca actggctttc tatctcggaa gttacgccgg aatacaactg agcaagcaag
      541 gctttttcga gcagatagac ctgattctgc cggtgccttt acataaaaac aaagagcgac
      601 ggaggggtta taaccaaagt gaacttatcg caaggggcct ggctcactgc agcggcttgc
      661 ccgttgatac cggttctctt ggtcgtgccg tggaaaatcc tacccaaaca aggcgcagca
      721 tttgggaacg aagccggaat gtgcagcaaa ttttcagatt aagcagtttc gaaaaacttg
      781 ccggtaaaca tgttttgctg gtggacgatg ttttaaccag cggctccacc atggaggctt
      841 gcgggaatgc tttgttggaa gcccccgggc ttagactgag tttttttgcc ctggctctgg
      901 cttaattctt tgagacactg tctttgtata ttgcaaaata tctgtacttt tgtccctgat
      961 ttaaaacagc ttgagaagat ctgagcaaaa aggtcaactc cctaattatg accgctatga
     1021 aaaagaaaac gatcctgacg acctttttga tttttggttt gcttgtccat tcccgtgcag
     1081 aagacattgt taaatcggga ttcaacctgg gagcattgcc ggccatatct tacaattcag
     1141 atttaggttt tcaatatgga gttatactga atttgtttca ctacggggat ggctcgcgtt
     1201 atcctgcttt tgatcattcc atgtatctgg aagtgtcgcg atataccaaa ggtacaggcg
     1261 tataccggct ttactacgac tcggaccgtt tgataaaagg agtgcggagt tttatcgatt
     1321 tgtcgtatat gaccaatctg atgcaggatt tcttcggcta taacgggttt cgttcggttt
     1381 acagtgtcga ttcggtggca gccaattcgg ccttttataa aatgcatcaa aataaatttc
     1441 atttaatggc cgattttcag ggaaatctcc cgatggacaa tttaaagtgg ttgttgggtt
     1501 acggcttgta taattacagc atagccccgg tcaaccttga gaaacttaac aagggtaaat
     1561 cccccgaaga gcagattccc gacaggcccg gcttgtatca gaaatacgtg gagtgggggc
     1621 tgatcagtcc cggtgagaag cacggcggaa tgcttaacct gttgaaggcc ggccttatgt
     1681 acgatagtcg taatgccaaa accaatccgg ataaaggcat ctttacggaa gcgctgctcg
     1741 aaatggcagc tcctttgctg aacgaaagcc cctatgtcaa ttacattgtg atgcaccgtc
     1801 aatatcagtc gctgatcaaa aatcggctga atgcagctat acgaattggt gtgcagggca
     1861 agatcggtaa taaggcagct cctttttatc gtaaacctca attgatttcc acttttgcta
     1921 cccggaccgt taccaccggt ttaggcggaa actcaactct gcgcggcatt ttgcaaaaca
     1981 gggtggtggg cgatgccttt tgcgctggcc aacgtggaat tacgctggaa aatgattaat
     2041 tttcgctggt ttaaccagaa tttttacatc gggaccaatt acttttggga tgccggttat
     2101 atc
//
LOCUS       DAOSGL010000355         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_53440_length_2078_cov_3.979239, whole genome shotgun sequence.
ACCESSION   DAOSGL010000355 DAOSGL010000000
VERSION     DAOSGL010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53440_length_2078_cov_3.979239"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKY68_09085"
     CDS             complement(<1..545)
                     /locus_tag="PKY68_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOH25220.1"
                     /translation="MNGWEAFLLGLVQGLTEYLPVSSSGHLEIGNYLLGTAGEENLTF
                     TVAVHFATVMSTLVVLWPEIWSLTKGFFKFKDNEETRYVLKILVSMIPVMLVGFFLKD
                     QVEALFGNGLLIVGIMLLLTAALLAFSYFAKPRIKESISWRDAFIIGIAQACAVLPGL
                     SRSGSTIATGLLLGNKKESVA"
     gene            complement(542..796)
                     /locus_tag="PKY68_09090"
     CDS             complement(542..796)
                     /locus_tag="PKY68_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOH25221.1"
                     /translation="MSKDFALSGRNLLWIGIGFLIVVLGFVLMMGAPSTEEAYNPDIF
                     STRRIVIAPTISFIGFVVVMVGILIRKPTKAKTEQDNLKA"
     gene            complement(805..1683)
                     /locus_tag="PKY68_09095"
     CDS             complement(805..1683)
                     /locus_tag="PKY68_09095"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOH25222.1"
                     /translation="MSKQKKKIGSRVFGSKLTAILSISLVLLLFGIMIIIGLFAKDLS
                     DTAKEHIRLTLVLDDNIKDVERISLEQNLNKAPWTNRLDYVSKEDALAELAEELGENP
                     LDLLGYNPLPASYEIYLKPEYACTDSIKGIEKTLKNKSYIQEIVYREELINLVNSNIL
                     SIGRLLLGIALILTLVSYMLIRNTVRLMIYSKRFLIHTMCLVGARQSFIRRPFVWENT
                     RGGIWGALIALGLLYMALSYLDSIFPGIMKMITVTDYVLIFVVMAILGMVITSLATVF
                     SVNRYLRMRSDDLYYI"
     gene            complement(1784..>2078)
                     /locus_tag="PKY68_09100"
     CDS             complement(1784..>2078)
                     /locus_tag="PKY68_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOH25223.1"
                     /translation="AAKHLPDDVPEKVKLARLAEMIGLQNELSAQSNQHEIGKTHTVL
                     VDSWSKRSRERLSGRTEQNKTVVFDKGTAKIGDFVQVRIVNSTSATLLGELVE"
BASE COUNT          617 a          513 c          399 g          549 t
ORIGIN      
        1 tgagcaacac tctccttttt gttacccagt aaaagtcccg ttgctatagt cgatcccgaa
       61 cgcgacaaac ccggcaatac cgcacatgcc tgagcaatac ctattataaa agcatctctc
      121 caggaaatgc tttcttttat tctgggttta gcaaaatacg aaaaggccag cagagcggct
      181 gtaagcaaca acataatacc aacgatgagc agaccgttgc caaacagagc ttctacctga
      241 tcttttaaaa agaaaccgac caacatgacc ggtatcatcg ataccagtat ttttagaaca
      301 tagcgggttt cttcgttgtc tttgaactta aaaaagccct tggtcaaaga ccagatttcg
      361 ggccaaagca ccaccaacgt actcatgacc gtagcaaaat gtactgctac ggtaaaagtg
      421 agattttctt ctcctgcggt acccagtaaa taattgccta tttcgagatg accgctgcta
      481 ctgaccggca aatattcggt cagcccttga accaggccca aaagaaaggc ctcccatccg
      541 ttcatgcctt caaattgtcc tgttctgttt ttgcctttgt gggctttctt attaaaatac
      601 cgaccatcac cacaacaaag ccgatgaaag aaattgtcgg agctatcact atgcgacgcg
      661 tactgaatat atcgggattg taggcttctt cagtcgaagg agcgcccatc atcagcacaa
      721 agcccaaaac gacaatcaga aagccaatgc ctatccacaa aaggttcctt ccacttaatg
      781 caaaatcttt actcatgttt ttaattaaat ataatacaaa tcatcggaac gcatcctcag
      841 gtaacgattt acagaaaaaa cggttgccaa agaggtaatt accatgccca gaatggccat
      901 aacaacaaag atcaacacat aatcggtcac ggtaatcatt ttcataatac cggggaaaat
      961 actgtcgaga tacgacaagg ccatatacag caaacccaag gcaataaggg ctccccatat
     1021 gcctccccgg gtgttttccc agacaaaagg cctgcgtata aagctttgcc tggctcctac
     1081 gaggcacatg gtatgaatca aaaaacgctt ggagtaaatc atcagacgaa cggtgttgcg
     1141 tatcaacata taagaaacca gcgtaagaat cagggctatt cccaataaaa gccggccaat
     1201 ggaaagtata ttgctgttca ccaaattaat cagctcctcg cgataaacga tctcctgaat
     1261 atagctttta ttttttaatg tcttttctat gccctttata ctgtccgtac aggcatattc
     1321 aggcttcaga tagatttcat agcttgccgg cagaggattg taaccgagca aatcgagggg
     1381 attctctccc aactcctctg ccaattcggc cagggcatct tctttgctca cataatctaa
     1441 tctattcgtc cagggggctt tgttcaggtt ttgttccaac gagatgcgtt cgacatcctt
     1501 gatattatcg tccagcacca aggtgaggcg aatatgttct ttggccgtat ccgacaaatc
     1561 tttggcaaat aggccaataa tgatcattat accgaaaagc agcaatacca aactgatgct
     1621 caatatggca gtcaatttgg aaccgaagac ccggctccct atcttttttt tctgtttact
     1681 cattctgtaa aaactgcccg cgaaaggcaa aaagcaaact tccccccaaa ttgccgcaaa
     1741 agtacataat attcctgagg aaaagaacag ccattcggtg tttttattct acaagttctc
     1801 ccaataaggt ggcagaagta ctgttcacta ttctaacctg cacaaaatcc ccaattttgg
     1861 cagtcccctt atcgaaaacc accgttttgt tttgttccgt acgcccggaa agacgctctc
     1921 gtgaacgttt ggaccaggaa tctaccagca ctgtatgtgt tttcccgatt tcatgttgat
     1981 tgctctgcgc cgagagttca ttttgcaagc cgatcatttc agctaaacgg gccagtttaa
     2041 ctttttcggg aacatcgtcg ggcaagtgtt tggccgca
//
LOCUS       DAOSGL010000356         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_53540_length_2076_cov_2.796141, whole genome shotgun sequence.
ACCESSION   DAOSGL010000356 DAOSGL010000000
VERSION     DAOSGL010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53540_length_2076_cov_2.796141"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1080
                     /gene="corA"
                     /locus_tag="PKY68_09105"
     CDS             142..1080
                     /gene="corA"
                     /locus_tag="PKY68_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOH25224.1"
                     /translation="MAEIKLFYHENGRIKVSRSLDFLKQTPIEKFLWIDLNNVDEEVE
                     SQLENFLKIYIQEEEEMEEIEMSSRYIETSDTLVVNSNFLLSDYETDTVSFILKDNIL
                     VTVHDEELPSLQEAAKKITTSPRNYPSGYHVMVALLETRVERDADLIEDIIDKITVLS
                     DTVRDADEDVLMDITQLQEQILAIRQNIIEKQRSISNLLKSELMPQELSAKLTIIVKD
                     INSLIDHTKFSFERLDYLQDTFLGLVNIDQNKIIKIFTIVSVIFMPPTLVASIYGQNF
                     VKMPFLNNAAGFWISMGMMVLFSGAILLYFKKKRWL"
     gene            complement(1081..1626)
                     /locus_tag="PKY68_09110"
     CDS             complement(1081..1626)
                     /locus_tag="PKY68_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOH25225.1"
                     /translation="MKLLLFLAPGFEEIEAITVLDIARRAGLSIRSVSISNEKAVSGA
                     HQISLNADLTFDEVNFDEAEALILPGGMPGTNNLNAHAGLKKQLVNFARNPDKTIAAI
                     CAAPLILGDLGLLDGKKATCYPGYESHLSRAKLSRKPVVRDGNIITADGPAHAMLFAI
                     KLVDYLGNTFLADDITKGLNY"
     gene            complement(1684..>2076)
                     /locus_tag="PKY68_09115"
     CDS             complement(1684..>2076)
                     /locus_tag="PKY68_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOH25226.1"
                     /translation="FGSKQGQQSESQGFGQEGSGNQGDIQGSPESANISGGGSGYGNF
                     SLDGRSVVGSLPRPSYSIETEGVVVVRITVNSAGTVISAGIDLLGTDTDNTELRNAAL
                     TAAKKAKFNAIDGTRNQTGTITYRFQLR"
BASE COUNT          557 a          458 c          486 g          575 t
ORIGIN      
        1 ccctttttat actgaagcta ttttcgacaa gcgtcccgag cagctcagtg tcaagcaatt
       61 tgaatacctg accgggctga taagcgattt ttattacagt aattcacctt ccggtgcagg
      121 catcggacaa agttaagaga tatggccgaa attaaacttt tttaccacga aaacggaaga
      181 atcaaagtaa gccgcagcct cgattttctc aagcagactc ccattgaaaa gtttctctgg
      241 atagacttaa acaatgtgga cgaagaagtc gaaagtcaat tggaaaattt cctgaaaatt
      301 tacatccagg aagaggaaga aatggaagaa atcgaaatga gctcccgtta catagaaact
      361 tccgacacgc tggtagtaaa ctcaaacttt ttgttgagtg attatgaaac cgatacagtt
      421 tcattcattc ttaaagataa tattttggta acggtacacg acgaagaatt gccttcgctg
      481 caggaggctg ccaaaaaaat aactaccagt ccgcgcaact accccagcgg ttaccacgta
      541 atggttgcct tgctcgaaac cagggtggaa agggatgccg accttattga ggatattatt
      601 gacaagatta ccgtgttgag cgataccgtc agggatgccg acgaagatgt gctgatggat
      661 ataactcagt tacaggaaca aattctggcc atacgtcaga acatcatcga gaaacaacgt
      721 tctatctcca atctgctcaa gagtgagctc atgccgcagg aattgtcggc caaacttaca
      781 atcatagtaa aggatatcaa ttctctgata gaccacacaa agtttagttt tgagcgtctg
      841 gattacttgc aggatacctt tttgggtttg gtaaacattg atcagaacaa gatcattaag
      901 atatttacca ttgtatcggt gattttcatg cctcccacac tggtggcaag tatttatggc
      961 cagaacttcg taaaaatgcc atttttgaac aatgcagcag gcttttggat ttccatgggc
     1021 atgatggtcc tgttctccgg agctatcttg ttgtatttca aaaagaagcg ttggctgtaa
     1081 ttagtagttc aaacccttgg taatatcatc tgccaggaag gtattcccta aataatcaac
     1141 aagtttgatc gcaaacagca tggcgtgagc cggtccgtcg gcggtaatta tgttgccgtc
     1201 tctgacaacg ggttttctgg acaatttggc cctgctcagg tggctctcat agccggggta
     1261 gcaggtggct tttttaccgt ccagcaggcc caggtctccc agtattaaag gagctgcgca
     1321 aatggccgct atggttttgt cgggattccg ggcaaagttg accaactgct tctttagccc
     1381 tgcgtgggcg ttgaggttgt tggttcccgg cattccaccc ggcagaataa gggcttcggc
     1441 ttcatcaaag ttaacttcgt caaaggtcaa atcggcattc aggctgattt ggtgagctcc
     1501 gcttactgct ttttcgttgc ttatggaaac agaacgaatg gaaagcccgg ccctgcgtgc
     1561 tatatccagt acagtaatag cttctatttc ttcgaagccc ggcgccaaga acaataaaag
     1621 tttcatatag gttcaattta ggtaggtaca aataggtatt gaaatcgcta tttcagtacg
     1681 acattatctt aactggaaac gataggtgat agtacccgtt tggttacgtg taccgtcaat
     1741 ggcattaaac tttgcctttt tcgctgccgt taaagcagcg ttgcgcaatt cagtgttgtc
     1801 ggtgtcggtg cccagaaggt ctatgccggc tgaaataacg gttccggcac tatttactgt
     1861 aatacgtact acaaccaccc cttcggtttc gatggaatag ctgggacggg gtaaactgcc
     1921 gacaacggag cggccgtcga gcgaaaagtt gccgtagccc gatccgccac cgcttatgtt
     1981 ggccgattcg ggactgcctt gtatatcgcc ctgattgccg ctgccttctt gtccaaaacc
     2041 ttggctttcg ctttgttgtc cttgtttgga tccgaa
//
LOCUS       DAOSGL010000357         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_54467_length_2052_cov_3.980471, whole genome shotgun sequence.
ACCESSION   DAOSGL010000357 DAOSGL010000000
VERSION     DAOSGL010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54467_length_2052_cov_3.980471"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /gene="recA"
                     /locus_tag="PKY68_09120"
     CDS             complement(<1..979)
                     /gene="recA"
                     /locus_tag="PKY68_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOH25227.1"
                     /translation="MSKEQTEQKEQKGPAAEKLKALQMAMEKIEKSYGKGSIMKMGDD
                     KVVDVPVISSGSIGLNAALGVGGFPRGRVIEIYGPESSGKTTLAIHAIAEAQKAGGIA
                     AFIDAEHAFDRFYAEKLGVDIANLWISQPDSGEQALEITEQLIRSSAVDIVVIDSVAA
                     LTPKAELEGEMGDSKVGLQARLMSQALRKLTATISKTNTTCIFINQLREKIGIMFGNP
                     ETTTGGNALKFYSSVRLDIRRIGQIKDGEEIIGNQTRVKVVKNKVAPPFRKAEFDIMY
                     GEGISKTGELIDLGSDLGIIKKSGSWYAYKDSRLGQGREAAKDCMRDNPE"
     gene            1308..>2052
                     /locus_tag="PKY68_09125"
     CDS             1308..>2052
                     /locus_tag="PKY68_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOH25228.1"
                     /translation="MVDIIFWLIPLASVTALIFAFYFYRQMKKKDEGTDLMKKIAGFV
                     REGAMSYLKQQYKVVTIVFIILALIFAAMAYFGLQNNWVPFAFLTGGFFSGLAGFLGM
                     KTATYASARTAHAARSSLNQGLQVAFRSGAVMGLVVVGLALLDISLWYLILNYFIDAA
                     SPEQKNMIITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPR
                     NPATIADNVGDNVGDVAGMGADLYESYCGS"
BASE COUNT          504 a          470 c          454 g          624 t
ORIGIN      
        1 attcgggatt gtcgcgcatg cagtctttgg cggcttccct gccctgcccc agtcgactgt
       61 ctttgtaagc ataccaggaa ccgctttttt tgataatgcc caaatcggaa cccagatcta
      121 ttaattctcc ggttttggaa atgccttcac cgtacatgat atcaaattcc gctttgcgga
      181 agggtggagc cactttgttc ttaaccactt ttacacgagt ctgatttccg atgatttctt
      241 caccgtcttt aatttgtccg atacgacgga tgtcgaggcg cactgaggaa taaaacttga
      301 gcgcattacc accggtagtt gtttcgggat tgccgaacat gatgccgatt ttttctctaa
      361 gctgattgat aaaaatacaa gtcgtgttgg ttttgctgat ggtagcggtg agtttacgca
      421 aagcctgaga catcagacga gcctgaagac ccactttgga atcgcccatt tctccttcca
      481 attcggcctt gggggtgaga gcagctacag agtcgatgac tacaatatcc actgctgaag
      541 agcgtataag ttgttcggta atttcgagtg cctgttctcc gctgtcgggc tgagatatcc
      601 ataaattggc aatatctaca cccagttttt cagcgtaaaa acggtcgaag gcatgttcgg
      661 catcaataaa agcagctata cctccggctt tttgcgcttc ggcaatagca tgaatggcca
      721 gggtggtttt gccggaggat tccggtccgt aaatttcgat tacccggccc cggggaaagc
      781 ccccaactcc cagagccgca ttcaaaccga tggaacctga ggaaataacc ggtacatcga
      841 caactttatc gtctcccatt ttcataattg agccttttcc gtagcttttt tctatttttt
      901 ccatggccat ttgcagagct ttcaattttt ctgctgctgg acctttttgt tctttttgtt
      961 ctgtttgttc ttttgacata tttgttaaga ttataatgtt tacggatcaa agataagaca
     1021 ctactttgcc accggcaaac aaagtgctgt taaatattaa taaatcatta tgtttaaaat
     1081 tctttttgtt taattttaat cttaattcct ctattaatga gtggcttaaa tgggtgttta
     1141 ttgtattcag ccctcgaagc attctatgcc ttacaaagtt ataaaaaaga tcggttaata
     1201 agacactata tactctttaa ataaatgtcg taaaataaat tccgctcaga tattgccatt
     1261 tttgcaagga gtttatactt ttgtccctac tctctaaaaa aaacaccatg gtagatatta
     1321 ttttttggtt aatcccgctg gcgtcagtca cagccttaat ttttgccttt tatttctaca
     1381 ggcaaatgaa gaaaaaagac gaaggaaccg acttaatgaa aaaaattgcc ggttttgtac
     1441 gagaaggagc gatgtcctat ctcaagcaac aatacaaggt cgtcaccata gtatttataa
     1501 tactggcctt gatttttgcc gccatggcct atttcggtct gcaaaacaat tgggtgcctt
     1561 ttgccttcct aaccggggga tttttctcgg gtttggccgg ctttctgggt atgaaaacag
     1621 ccacttatgc ctctgcacgc accgcacacg ctgcccgcag ttccctcaat cagggcttgc
     1681 aagtggcttt ccgaagcggg gccgtcatgg gtctggtggt cgttggtctt gccttgctgg
     1741 atatttcgct ctggtatctc attttgaatt attttataga tgcggccagc ccggagcaaa
     1801 aaaacatgat catcaccacc accatgctga cttttggcat gggtgcatct acccaagctt
     1861 tgtttgcccg cgtgggcgga ggtatttaca ctaaggcagc cgacgtaggt gccgacctgg
     1921 tgggcaaagt ggaagccgga atcccggaag acgatccccg caaccctgct accattgccg
     1981 acaacgtggg cgataacgtg ggcgatgtgg ccggtatggg agccgacctt tacgaatctt
     2041 attgcggctc ca
//
LOCUS       DAOSGL010000358         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_54694_length_2046_cov_4.960824, whole genome shotgun sequence.
ACCESSION   DAOSGL010000358 DAOSGL010000000
VERSION     DAOSGL010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54694_length_2046_cov_4.960824"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKY68_09130"
     CDS             complement(<1..382)
                     /locus_tag="PKY68_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOH25229.1"
                     /translation="MIYVIFIGFALLSWIVQSVLKNKFDKYSKIALPSGLSGKEVAEK
                     MLHEHGIYDVKVSHVQGQLTDHYNPLTKTINLSEAVYNSNSIAAASVAAHECGHAVQH
                     ATAYSFLTMRSKLVPVVNFASSWMH"
     gene            complement(388..1653)
                     /locus_tag="PKY68_09135"
     CDS             complement(388..1653)
                     /locus_tag="PKY68_09135"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOH25230.1"
                     /translation="MDVLLGLQWGDEGKGKVVDVLTPGYEVVCRFQGGPNAGHTLEFE
                     GHKYVLRSIPSGIFQGDKINVVGNGVVIDPALFMGEIDQLAKSGHKLNDRLMISKKAH
                     LILPTHRLLDAANEAAKGQGKIGTTGKGIGPAYTDKVSRNGLRVGDILHDFKRKYKEA
                     AALHLNRLKQMNFPVDSEELAAAEKEWFEGIDCLKQFKLIDSEHFLNKKIGEGKKILC
                     EGAQGTMLDIDFGSYPFVTSSNTVCSGACTGLGVAPRHIDRVFGIFKAYCTRVGSGPF
                     PTELFDENGKLMRDRGNEYGSVTGRPRRCGWIDLVALRYAVMINGVTDLIMMKSDVLD
                     SFDTIKACVAYRIGGKETESLPFDIQGKLEPVFTELPGWKTDMTKMRSEDEFPEEFNQ
                     YLSFLEDELGVRIKYVSVGPDRAQTIERY"
     gene            complement(1756..>2046)
                     /locus_tag="PKY68_09140"
     CDS             complement(1756..>2046)
                     /locus_tag="PKY68_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25231.1"
                     /translation="LHSSFHFGLGTLVTFPLWYLILFSTVWIISGTFWIAAAYLAVLP
                     LSLLIFFRSKVLIIKLYNRTRRFWLKVRKNPLLAASEKLRHEIVEVMDKLIF"
BASE COUNT          512 a          506 c          491 g          537 t
ORIGIN      
        1 aatgcatcca gctggaagca aaattcacca ccggcaccag tttggagcgc atggtcaaaa
       61 aagaataggc ggtggcgtgc tgtacggcgt gtccgcattc gtgggcagcc accgaagcgg
      121 cagcaatact gttgctgtta taaaccgcct cgctcaggtt gatggttttg gtcagcggat
      181 tgtaatggtc ggtgagctga ccctgcacat gagacacttt gacatcgtaa atgccgtgtt
      241 catggagcat cttctcggct acctccttgc cgctcaggcc tgagggaagg gctattttgc
      301 tgtatttatc aaatttattt ttcaatacgc tttgcactat ccagctaagg agggcaaagc
      361 ctataaaaat cacatatatc atcataatca ataacgttct atggtttgag cccggtcggg
      421 acctaccgaa acatatttga tgcgaacacc caactcgtct tcgagaaaac tcaggtattg
      481 attgaattct tcgggaaatt cgtcttcgga ccgcattttg gtcatgtccg ttttccatcc
      541 cgggagttca gtaaataccg gttccagttt tccctgaata tcgaaaggca ggctttcggt
      601 ttctttgccg ccaatcctgt aggctacgca ggctttgatg gtatcgaagc tgtcgagtac
      661 gtcgcttttc atcataatca ggtcggtcac tccgtttatc attacggcat aacgcagagc
      721 caccaggtct atccagccgc aacggcgcgg acgacccgtt accgagccgt attcgttgcc
      781 ccggtcgcgc atcagtttgc cgttttcgtc gaagagctct gtggggaaag gaccgctgcc
      841 cacccgggta caataagctt tgaaaatacc aaacacccgg tcgatgtggc gaggagctac
      901 gcccaggccg gtgcaggctc ccgagcaaac cgtattggac gaggtcacaa agggataaga
      961 cccgaaatca atatcgagca tggtgccttg tgcaccctcg cataaaatct ttttgccttc
     1021 accgattttt ttgtttagga aatgctcact gtcgatcagt ttaaattgct ttaggcagtc
     1081 gattccttcg aaccattctt tttcggccgc ggccagttct tcggagtcga ccgggaaatt
     1141 catttgtttc agtctgttca ggtgcagggc ggcagcttcc ttgtatttgc gcttgaagtc
     1201 atgaagtata tcgcccaccc tgagcccgtt gcggctcact ttgtcggtat aggcagggcc
     1261 aatgcctttg cctgtggtgc cgattttgcc ctgacctttg gcggcttcgt tggctgcatc
     1321 cagcagacgg tgggtaggca aaatcagatg agcttttttg gaaatcatca atcgatcgtt
     1381 cagcttgtgc ccactcttgg caagttgatc gatttctccc atgaacaaag caggatcaat
     1441 cactacacca ttgcccacca cattaatttt gtccccctgg aaaatacccg aaggaatgga
     1501 acggagtacg tatttatgcc cttcgaattc cagtgtgtgc ccggcattgg ggccgccctg
     1561 aaaacggcat accacttcat aaccgggtgt cagcacgtcc accaccttgc cttttccctc
     1621 gtctccccat tggaggccta ataatacatc catgataaaa taatgattac cagtagtgtg
     1681 cacataaaag ggcacgaaca tagctatgtt cgtgcttttc tctcaaaaaa accggctaca
     1741 aagataaaaa atattttaga atatcagttt atccataacc tcaactattt catgacgaag
     1801 tttttcggag gctgctaaca gagggttttt ccttactttg agccagaagc ggcgagtgcg
     1861 gttgtagagt tttataatca acactttgga tcggaagaaa atgagcagac tcaagggaag
     1921 tacagccagg taggccgccg ctatccagaa agtgccgctt attatccaaa ctgttgagaa
     1981 cagaattaaa taccagagcg ggaaagtgac cagggtgccc aggccaaaat gaaaagaaga
     2041 atgcag
//
LOCUS       DAOSGL010000359         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_54874_length_2042_cov_3.120785, whole genome shotgun sequence.
ACCESSION   DAOSGL010000359 DAOSGL010000000
VERSION     DAOSGL010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54874_length_2042_cov_3.120785"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..>2042)
                     /locus_tag="PKY68_09145"
     CDS             complement(16..>2042)
                     /locus_tag="PKY68_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH25232.1"
                     /translation="VTRNLRDDGVMPPEESNVFSPGFLSLIKSPFLSPYQYDTNKNLS
                     NFLSDADDYLNEVLGSRASLANPLYIFENGEARNKNYFENSLINLAIRPAYSFNKNFS
                     VKGHFSYTLVNTNENYYIPMTGVPKIIIPNVGTAENLRKSLYSSQISLFADLRMLWQK
                     KRGANDTRLMAGGRYMNDDFSGNTQMGYDTGNDKTPNMNANLNFKKVDGAEDVWRSLS
                     YYANLDYNYAEKYYLHAGLSIETSSRFGREVQGGIRMGGVSWGLFPSAAAAWVLSSED
                     WFRLRSVDYLKLNLAYDISGNDDIDYYAARTYFGSSVFMDRVTGLTIQNIGNTSIQWE
                     STQKGGAGLEASLFQERVGIQLNAFLAKTDNLLTLKKLASVTGLDYNWSNEGSLTNRG
                     FDLALNFKLVNRNKFNWGMGFSLGHYKNEITALPDDESFITKLYGAEILSEKGRPAGL
                     FYGYQTKGVYASSEEAAADGLYILSKTGSKVYFGAGDVIFVNNRDDSKEINADDKVVI
                     GDPNPDLYGNIFSNIHYGRFSLDLILNYSLGNDAYNYQRSQLESGSKFYNQSLALLNR
                     WTNEDQKTTIPRISYLDQMGNSRFSDRWIEDASYLRLETVRLSYSLPLNTTFLNGLTL
                     WASGNNLWTLTRYLGSDPEFSSGNAVLCQGIDRGTLAFGPSVVLGIKLNL"
BASE COUNT          536 a          472 c          481 g          553 t
ORIGIN      
        1 tcatgattaa gagatttaga ggttgagttt gatgcctaaa accacactgg gaccaaaagc
       61 cagtgtacca cggtctatgc cttggcagag caccgcattc ccgcttgaga actcaggatc
      121 gctgcccaaa tagcgggtca gggtccataa attgttgccc gaagcccaaa gagtcagtcc
      181 attcaaaaag gttgtgttca aaggaagact gtagcttagc ctgactgttt ccagacgcag
      241 atacgaggca tcctcgatcc atcgatccga aaaacggctg ttgcccattt ggtccagata
      301 cgaaattctg ggtatcgttg ttttttgatc ttcgttggtc cagcggttca ataaagccag
      361 gctttgattg tagaacttgc taccgctttc gagttgagaa cgctggtagt tataggcatc
      421 gttgcccaga gaataattca gaataagatc gaggctgaag cgcccgtaat ggatattgct
      481 gaatatattt ccgtaaagat cggggttagg atcgccaata accaccttgt cgtcggcatt
      541 gatttccttg ctgtcgtccc ggttgttaac aaaaatcaca tcgccggcac caaaatatac
      601 tttggagccg gttttgctca gaatatataa accgtcggca gcggcctctt cggaactggc
      661 gtaaacccct tttgtctgat aaccgtaaaa caaaccggca gggcgacctt tttcggaaag
      721 aatttcggct ccgtacaact tggttataaa ggattcgtcg tcgggtaaag cggtaatttc
      781 gtttttataa tgtcccaggc tgaaacccat tccccagttg aatttattcc ggttgaccag
      841 cttgaaattc agcgccagat caaaacctct gttggtcaaa gagccttcgt tgctccaatt
      901 gtaatcaagt ccggtgacac tcgcgagttt cttaagggta agcaaattat cggtcttggc
      961 caggaaggca ttcaattgta tgccaacacg ttcctgaaac aggctggctt ccagaccggc
     1021 tcctcccttt tgggtgcttt cccattggat tgaagtattg ccaatgtttt gaatagtcag
     1081 accggtcact ctgtccataa agaccgaaga gccgaaataa gttctggccg cataataatc
     1141 aatatcgtcg ttacccgaaa tatcgtaagc caggttcaat ttaagataat cgaccgaacg
     1201 cagcctgaac caatcttcgg aactcaacac ccaggctgca gccgccgagg ggaacaaacc
     1261 ccagcttact cctcccatcc ggatgcctcc ctgcacttcc cggccaaatc gggaagatgt
     1321 ttcaatgctt aaacccgcat gcaggtaata cttttcggcg taattataat ccaggttggc
     1381 gtaataactc aatgaacgcc aaacgtcttc ggctccgtct acttttttga aattaagatt
     1441 ggcgttcatg ttgggggttt tgtcgttgcc tgtatcgtag cccatttggg tattaccgga
     1501 aaagtcgtcg ttcatgtaac gccctccggc cataaggcgg gtgtcgttgg ctccccgctt
     1561 tttctgccac aacatcctta agtcggcaaa aagagaaatc tggctcgaat agagcgattt
     1621 tctcaggttt tctgccgtac cgacattggg aataattatt ttaggtacac ccgtcatggg
     1681 aatgtagtaa ttttcattgg tattgaccaa ggtgtaactg aaatgtccct tgacgctgaa
     1741 atttttgtta aaggaataag caggcctgat ggccagattg atgaggctgt tttcgaaata
     1801 atttttatta cgggcctcgc cgttctcaaa aatatagaga ggattggcca gagatgccct
     1861 ggaacccaac acttcgttga ggtaatcgtc ggcatcggac aaaaaattcg aaagattctt
     1921 gttggtatcg tattgatacg gacttaaaaa gggcgacttg atcagactca gaaagccggg
     1981 agaaaacacg ttggattctt cgggaggcat aacaccgtcg tctctgaggt tgcgggtaac
     2041 gt
//
LOCUS       DAOSGL010000360         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_55234_length_2033_cov_3.910010, whole genome shotgun sequence.
ACCESSION   DAOSGL010000360 DAOSGL010000000
VERSION     DAOSGL010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55234_length_2033_cov_3.910010"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PKY68_09150"
     CDS             complement(<1..735)
                     /locus_tag="PKY68_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOH25233.1"
                     /translation="MKQLEINLTKVYDLVPQSEITALRPACQKAMDLLNNGKGKGSDF
                     LGWLHLPSLTDEKLLSDIQKTADLLREKCEVVVVAGIGGSYLGAKAVIDALNNSFDWL
                     KTKRENPVVVYAGHNISEDYLFELSCFLEGKRFGIINISKSGTTTETALAFRLLKTKL
                     EKQVGKEAARERIVAITDASKGALRQLAQQEGYKTFVIPDSVGGRYSVLTPVGLLPIA
                     VAGHDIRELLAGALAMEKRSETAFEQN"
     gene            complement(806..1807)
                     /locus_tag="PKY68_09155"
     CDS             complement(806..1807)
                     /locus_tag="PKY68_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOH25234.1"
                     /translation="MEFPDRMCVMGGGSWATAIAKMLLMNPQARINWYMRRASQIRDF
                     KRFGHNNSYLTTIPFDINRIRFYSDINVAVKNSDTLVLATPSPYLKLHLKKLQQDVSD
                     KFILSGIKGIVPDENMLVSQYFNKVYHVPHENIATIAGPCHAEEVALEHLSYLTIACS
                     DKEKARAIARSFECKFVRTTITDDVFGIEYASTLKNIYAIAAGICHGLKYGDNFQAVL
                     LSNAIQETNRFTQAAFPQNRKICESVYLGDLLVTAYSKFSRNRTFGTMIGKGYSVKTA
                     HMEMEMIAEGYYATKCIKEINEELYHVNMPILDAVYNVLYERISPSIEIKLLSESLR"
     gene            complement(1862..2033)
                     /locus_tag="PKY68_09160"
                     /pseudo
     CDS             complement(1862..2033)
                     /locus_tag="PKY68_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          485 a          474 c          501 g          573 t
ORIGIN      
        1 attttgctca aaagccgttt cgctgcgttt ttccatagcc agggctccgg ccagtaattc
       61 acgaatatcg tgaccggcta cagcaatggg cagcaagcct accggggtta gtacggaata
      121 gcgtccgcct acgctgtcgg gaataacaaa agttttgtag ccctcctgtt gggccagttg
      181 gcgcaaggcc cccttggaag catcagttat ggccactatg cgttcgcggg ctgcttcttt
      241 gcctacttgt ttttcgagct tggtcttgag caggcgaaaa gccagggcag tttcggtggt
      301 agtgcccgat ttggaaatat tgatgatgcc aaagcgcttg ccttctagaa agcagctcaa
      361 ttcaaagagg taatcttcgc tgatgttatg gccggcgtaa accactacag gattttcccg
      421 tttagttttg agccagtcaa aactgttatt tagcgcatcg ataacagcct tggctcccag
      481 gtaactgcct ccgataccgg ccaccaccac tacctcgcat ttttcgcgca ggaggtcggc
      541 ggttttctga atgtccgaca ataatttttc gtccgttagg gagggcagat gcagccagcc
      601 caaaaagtcg ctgcctttac ctttgccgtt gttcaacaaa tccatagcct tttgacaggc
      661 agggcgcaaa gccgttattt cgctttgagg aaccaagtcg taaaccttgg ttaaatttat
      721 ttcaagttgt ttcatatgct aatggtttaa tagtgattat atcaaaagac cgctctcaag
      781 ataagcattt tctgttaacg gagtattaac ggagtgactc gctcagcaat ttaatttcga
      841 tactgggaga aatacgttcg tacagcacgt tatacaccgc atccaatata ggcatattga
      901 cgtgatacaa ttcttcgttg atttctttga tgcatttggt ggcataatag ccttcggcaa
      961 tcatttccat ttccatatgc gcggttttta ccgaataacc cttaccaatc atggtaccaa
     1021 aagtgcggtt gcggctgaat ttagagtaag ccgtcaccag caggtcgccc aggtagaccg
     1081 attcgcaaat ttttcggttt tgcggaaagg ccgcctgggt aaagcggttc gtttcctgaa
     1141 tggcattcga aagcagtacg gcctggaagt tgtctccgta tttaagtccg tggcagatac
     1201 ctgcggctat ggcgtagata ttcttgagtg tcgaggcgta ttctatgccg aatacatcat
     1261 cggtaatggt ggtacgcaca aatttacatt caaagctgcg ggctattgcc ctggcctttt
     1321 ccttgtcgct gcaggcaatg gtcaggtaag agaggtgttc cagcgccact tcttcggcat
     1381 ggcagggccc ggctatggta gcaatgtttt cgtggggaac atggtaaacc ttgttgaaat
     1441 attgcgaaac caacatgttc tcgtccggca ctataccctt gatgcccgaa agaataaatt
     1501 tatcgctgac gtcctgttgt aattttttaa ggtgaagctt gaggtaaggc gagggcgtgg
     1561 ccagtaccaa agtatcggag tttttgacgg ctacgttgat gtcgctatag aaacggatac
     1621 ggtttatgtc aaagggaata gtagtcagat acgaattgtt gtgtccgaaa cgtttgaaat
     1681 ctcttatctg agaagccctg cgcatatacc agttgatcct ggcctgggga ttcatcagca
     1741 gcatcttggc aatagccgtc gcccagctgc ctccacccat tacacacatt ctatcgggaa
     1801 attccatacg gttttgtgtt ttttgttaaa cctgagcctt cggctctgtt ttgggagccg
     1861 gtttttcggg tcgcatttgg gggaagaaca gaacttcctg aatagaactc tgtccggtaa
     1921 gcatcatcac taaacgatcc atgccgatac ccatccccga agtcggcggc atgccgtatt
     1981 ccagggcacg gataaagtcg tgatcaataa acatggcctc gtcgtcgccc ttt
//
LOCUS       DAOSGL010000361         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_55277_length_2032_cov_4.398584, whole genome shotgun sequence.
ACCESSION   DAOSGL010000361 DAOSGL010000000
VERSION     DAOSGL010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55277_length_2032_cov_4.398584"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKY68_09165"
     CDS             <1..223
                     /locus_tag="PKY68_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25235.1"
                     /translation="ETPRLSLGFSMAGHTSQEALFEARWITKKTGTQILSIGVFTYPS
                     TRLLLFINFPHYNPFLPSLKEHTLLGYDG"
     gene            complement(317..1576)
                     /locus_tag="PKY68_09170"
     CDS             complement(317..1576)
                     /locus_tag="PKY68_09170"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOH25236.1"
                     /translation="MQSIEQFNFSGKKVFVRVDFNVPLDENKNITDDTRIRAAMPTLK
                     KIIKEGGMPIIGSHMGRPKKNPDPKYSLKAIQQHVADCLGAPVHFAEDCQKADAQVAA
                     LKPGEALLLENLRFYAEEEGKPRDLAEDASDEEKAAAKKIVKESQKAFTKKLASYADC
                     YVNDAFGTAHRAHASTALIADYFDDNNKMFGYLMNSEVQAVEKVMRDIVRPFTAIMGG
                     AKVSSKIDIIENLLDKVDNLIITGGMAFTFVKAMGGSIGNSICENDKLDLALELMEKA
                     KQKGVKLVLPIDSKVGDAFSNEANTQFVSIDAIPDGWEGMDIGPKTEALFAEVIENSK
                     TILWNGPAGVFEFENFTHGSKSVGEAVVEATKKGAFSLVGGGDSVACVNKFGLAKGVS
                     YVSTGGGALLEAIEGKVLPGIKAIKGE"
     gene            complement(1646..1960)
                     /gene="rhaM"
                     /locus_tag="PKY68_09175"
     CDS             complement(1646..1960)
                     /gene="rhaM"
                     /locus_tag="PKY68_09175"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOH25237.1"
                     /translation="MKRVAFKMKMIPGMKEEYIKRHDQIWPEIAELIKKSGVSDYSIF
                     LDEETNILFGVQKVEGNLSSQDLGFNEIQKKWWDYMADIMETNPDNSPITIPLVEVFH
                     LE"
BASE COUNT          530 a          483 c          406 g          613 t
ORIGIN      
        1 agaaacaccg agactcagct taggtttttc aatggcgggc cacacttctc aggaagcgct
       61 tttcgaagca aggtggatta caaagaagac cggcactcaa atcctgtcca tcggagtttt
      121 cacatatcca tcgacacggc ttcttctttt tatcaacttt ccccactaca atccattttt
      181 accatcccta aaggaacata ctttgctagg ctacgacggg taatttattt catacctgca
      241 acaaacaaag agaccgcccc tggcgcaccc cgggagcagt ctcttctaca tattagattt
      301 aaggtgtttc agtattttat tctcctttaa tagctttgat acctggcaaa actttgcctt
      361 ctatggcttc gagtaaagca cctccgccgg tagaaacata actaactccc ttagccaggc
      421 cgaatttatt gacacaggct acggaatcgc ctcctcccac caacgagaaa gcgccttttt
      481 tggttgcttc tacaacggct tcaccgacgg atttggatcc atgagtgaaa ttttcgaact
      541 caaaaacgcc ggcggggcca ttccacaata tggtcttgga attttcaatg acttcggcga
      601 aaagggcctc ggttttgggg cctatatcca tgccttccca tccatcgggt atggcgtcaa
      661 tcgatacaaa ctgagtattg gcctcattac tgaaagcatc gccaaccttg gaatcaatcg
      721 gcaagaccag tttaacgcct ttttgcttgg ctttttccat caattccaga gccaaatcaa
      781 gtttatcgtt ttcgcaaata gaattgccga tactgccccc catggccttg acaaacgtga
      841 aagccatacc gcctgtaatg ataaggttat ctactttatc caacagattt tcgatgatat
      901 ctattttgga agaaaccttg gctcccccca tgatggccgt aaaaggacgt acaatatcgc
      961 gcattacttt ttcaacggcc tgcacttcac tgttcatcag ataaccgaac attttgttgt
     1021 tatcatcgaa ataatcagca ataagagctg tagaagcatg agcacggtga gctgtaccga
     1081 aagcgtcgtt tacatagcaa tcggcgtaag aagccaattt tttggtaaaa gctttctggc
     1141 tttccttgac tattttttta gcagctgctt tttcttcgtc gctggcgtct tcggccaaat
     1201 cacggggttt accctcttcc tcggcgtaaa aacgcaggtt ctccaacagc agggcttcgc
     1261 cgggcttcaa agcagctacc tgagcgtcgg ccttttgaca atcttcggca aagtgtacgg
     1321 gtgcacccag acaatcggca acgtgttgct gaatagcctt taacgaatac tttggatcgg
     1381 gattcttttt gggacgcccc atgtgggaac ctatgatcgg cattccgcct tccttgatga
     1441 ttttcttcaa tgtaggcatg gcagcgcgaa tacgcgtgtc atcggtgatg tttttatttt
     1501 catccagagg gacgttgaaa tccaccctga caaaaacctt tttccctgaa aaattaaatt
     1561 gctctatcga ttgcataaaa gtatgaagta agacgtcgcg aagatagaga aatattctgt
     1621 tatatgctat tttctccgga tttttttatt ccaggtggaa aacttctacc aaaggtatag
     1681 tgatcggaga attgtcggga ttggtttcca taatatctgc catataatcc caccattttt
     1741 tttgtatttc attgaaacct agatcctgag aagacagatt tccttctact ttttgaacac
     1801 caaacagaat attggtttct tcatccaaaa aaatggaata atcgctgact ccgctttttt
     1861 tgatcagttc ggcaatttcg ggccaaattt gatcgtggcg tttgatgtac tcctccttca
     1921 tgccggggat cattttcatt ttaaaggcta ctcttttcat gactttacta tttgcgagtg
     1981 ttaagatgat cggtgctttc tcatttaagg tatttatcca gccaggcaaa aa
//
LOCUS       DAOSGL010000362         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_55355_length_2030_cov_3.939241, whole genome shotgun sequence.
ACCESSION   DAOSGL010000362 DAOSGL010000000
VERSION     DAOSGL010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55355_length_2030_cov_3.939241"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..>2030)
                     /locus_tag="PKY68_09180"
     CDS             complement(49..>2030)
                     /locus_tag="PKY68_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH25238.1"
                     /translation="TDNEMLASGAPTLWYSRDLLNWYNYTLDIPSLESINLRNFWAPD
                     IVKGDDGRYYLYFGNCQAGCNIYGYVSSTPVGPWTKLHQDDTPVIGSGFPRRGFPSLD
                     AQFFRDDDGKIYAYWGTWVHYNGGYAVGELDSKSMSRMINGKNIPLEQTPEPFEAAYV
                     LKHKDKYILMYSSASCHDASYNVRYSYADNPYGPFTPGDNNPILSTNADQSVHGPGHH
                     SVLKQGEDYYIVYHRHDYPMTRGGMARQVCIDSMIFENDSTIRKVIPSHRGIADIFPS
                     ELPADLAFGAKTKASSYYRMNSPGYDYTYKPSFATDNNNATMWKAADNSFPQTLEIDL
                     GSVQKISRVMTQFEFASYYYQYLLEYSTNGKKWAVYADRSDNRVSGSPMIDDKEVEAR
                     FLRLKVLGAEKTGIYAAVWNIKAYGSSFDIPLGLVNKASEAGPGAASKREMLLGFEIG
                     KKSKAENLSSLPNLGTLGGNFTLRGEVSLGTDDLGVQALEFTRGSLVLDVPVPQRLAW
                     NGSFTVATWVRNPEVGREGDCLFSWCDRHALRLANSYNALFYNRGPYGAMAHLDGHFD
                     MRYRNLPAENEWHHIVVTFDGVLEKVYVDGVLDNVQSMILASAVDRARFIVGASDVGE
                     HFSGYMASLRMYDYALSPKELSKLMKKTRPENLKK"
BASE COUNT          508 a          540 c          503 g          479 t
ORIGIN      
        1 aaaagacgca aacgcgtgct ggtaaaaatt ataggggaat aaaccacatt atttcttcaa
       61 attctcaggc ctggtcttct tcatcaattt gctgagttcc ttcgggctga gcgcgtaatc
      121 gtacatacgc agcgaagcca tataaccgct gaaatgttct cccacatctg aagctcccac
      181 tataaagcgg gctctatcca ctgccgaagc caggatcatg ctctgaacgt tgtcgaggac
      241 tccgtctaca tatacttttt ccaacacacc gtcgaaagta accacgatat gatgccattc
      301 gttttcggcc ggcaaattgc ggtaacgcat atcgaaatgg ccgtccagat gggccatagc
      361 cccgtacggg ccgcgattgt aaaacaaagc attgtaggaa ttagccaggc gcagggcgtg
      421 gcgatcgcac caggagaaca ggcagtcgcc ttcgcgccca acctcgggat tccttaccca
      481 ggtagccacc gtaaacgagc cgttccacgc aagcctctga ggcacaggca catccaagac
      541 taaagatcca cgggtaaatt caagagcttg cacgcccaag tcgtccgtgc caagcgacac
      601 ttcgcccctg agtgtaaagt ttccgcccag cgtacccagg ttgggcaggc tgcttagatt
      661 ctctgcctta ctctttttgc cgatttcaaa gccgagcaac atttcccgct tgcttgcggc
      721 tcccgggcct gcttcggatg ccttgttgac cagacccagg ggaatgtcga aggaggagcc
      781 gtaggcctta atgttccaaa cagcagcata gataccggtt ttttcggccc ccaatacttt
      841 tagtctcaaa aagcgggctt ccacttcctt gtcgtcgatc atggggctgc ccgaaacccg
      901 gttgtcggaa cgatcggcgt aaacagccca ttttttgccg ttggtagaat attccaacag
      961 atactgataa taatacgagg caaactcaaa ctgagtcatt actctgctga ttttctgcac
     1021 actccccaaa tcaatctcca gggtctgggg aaaagaatta tcggccgcct tccacatggt
     1081 ggcgttgttg ttgtcggttg caaacgaagg cttgtacgta tagtcataac ccggactgtt
     1141 catgcggtaa tacgaagagg ccttggtttt agctccgaag gccagatcgg ccggcaactc
     1201 cgaaggaaaa atatcggcta tgccccgatg cgaaggaatc actttacgaa tggtggaatc
     1261 gttctcaaaa atcatgctgt cgatgcaaac ctgccgcgcc ataccgcccc gggtcatggg
     1321 ataatcgtgg cgatgatata cgatataata atcttcgccc tgcttcaaca ccgaatgatg
     1381 tccggggccg tgtaccgact ggtcggcatt ggtgctgaga atgggattgt tgtcaccggg
     1441 agtaaaagga ccatagggat tgtcggcata ggaatagcgc acgttgtaac tggcgtcgtg
     1501 gcaggaagcg ctggaataca tcaggatata tttgtcctta tgcttgagca cgtaagctgc
     1561 ctcaaaaggt tccggtgtct gctccagggg aatatttttt ccgttgatca tgcgggacat
     1621 gctcttacta tcgagttctc ccacggcgta gccgccgttg tagtgcaccc aggtgcccca
     1681 gtaggcgtag atttttccat cgtcgtccct gaaaaactga gcatccagcg aaggaaagcc
     1741 cctgcgagga aagccgctgc cgattaccgg agtgtcgtcc tgatgcaatt tagtccaggg
     1801 tcccaccggc gtggaagaaa catagccgta aatattacat cctgcctgac aattaccaaa
     1861 atacaggtaa tagcgcccgt cgtcgccttt cacaatatcg ggagcccaaa aattccgcaa
     1921 attgatggat tccagcgagg gtatatccaa agtataattg taccaattca acagatcgcg
     1981 actataccac agcgttggcg cccccgatgc caacatctcg ttgtcggtgg
//
LOCUS       DAOSGL010000363         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_55440_length_2028_cov_4.108464, whole genome shotgun sequence.
ACCESSION   DAOSGL010000363 DAOSGL010000000
VERSION     DAOSGL010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55440_length_2028_cov_4.108464"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKY68_09185"
     CDS             <1..452
                     /locus_tag="PKY68_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOH25239.1"
                     /translation="TFQREVYSDLTGERGTLMGAIQGLLLAQYEVLRANGHTPSEAFN
                     ETVEELTQSLMPLFAQKGMDWMYANCSTTAQRGALDWMGPFHDAVKPVFEKLYKEVAN
                     GNEAQRSIDSNSKPDYREKLEEELRQLRESEMWQTGAVVRKLRPENN"
     gene            complement(449..1642)
                     /locus_tag="PKY68_09190"
     CDS             complement(449..1642)
                     /locus_tag="PKY68_09190"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOH25240.1"
                     /translation="MNPVSLRLASLAPSETLAMSQKSNELKAMGFDVINMSVGEPDFF
                     TPDHIKLAAKQAVDNNFSFYSPVPGYPALRKAIVEKLKKENGLDYTVDQIVCSNGAKQ
                     SVCNVLLSIISPGDEVIVPAPYWVSYVEMVKLAEGKNVIVSAGIEQDFKITAAQLEAA
                     ITPKTKALILCSPSNPTGSVYSKEELQALAAVLAKHPQVYILSDEIYEHINYLGKHES
                     IAQFESVSERVIVINGVSKAYAMTGWRIGWIAAPQWIASACNKLQGQYTSGPCSVSQE
                     AARAAYTGDQACVAEMRTAFERRKNLIVKLMREVPGFKVQEPKGAFYVFPDCSAYIGK
                     SFKDKTITNATDLAMFLLEEAHVAAVGGVAFGAPDCLRFSYATSDENIVKAIDRIKVA
                     LAKLA"
     gene            complement(1690..>2028)
                     /locus_tag="PKY68_09195"
     CDS             complement(1690..>2028)
                     /locus_tag="PKY68_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOH25241.1"
                     /translation="KNETLEPVEKNIRVVASKKLTAFPKTVSEPISGIEWAEGPTPLQ
                     IGKYTYVYWDKYRNQRYGAARCKDLKKGVWDDISKIISFPSGVRHGTAFEVEEEVLNN
                     LLKMDENPQN"
BASE COUNT          497 a          482 c          521 g          528 t
ORIGIN      
        1 ctacctttca gcgcgaagtg tattccgatc ttacgggcga acgcggtact ctgatgggcg
       61 ccattcaggg cctgttgctg gcacaatacg aagtgctcag agccaacgga catacgccct
      121 cggaagcctt caacgaaacc gtcgaagaat tgacgcaatc cctgatgccc ttgtttgctc
      181 aaaaaggaat ggactggatg tatgccaatt gctccaccac tgctcaacgc ggcgccctcg
      241 attggatggg acctttccac gatgcagtga agccggtctt cgaaaaatta tataaagaag
      301 tggccaacgg caacgaagct caacgctcca tcgactccaa ctccaaaccc gattacaggg
      361 aaaaactcga agaagaactc cgccaattgc gcgaaagcga aatgtggcag accggtgccg
      421 tggtacgcaa attgcgcccc gaaaacaatt aggccaattt agccaaagcc actttaatac
      481 gatcgatcgc tttaacaatg ttttcgtcgg aggtggcgta ggagaaacgc aaacaatcgg
      541 gtgcgccgaa tgctacgccg cctaccgccg ctacatgagc ttcttcgagc agaaacatag
      601 ccagatcggt ggcattggtt atggttttgt ccttgaacga tttgcctata taagcgctgc
      661 aatcgggaaa aacatagaag gctcccttgg gctcctgtac cttgaaaccg ggaacttcgc
      721 gcatcaattt tacgatgagg ttcttacgac gttcaaaggc tgtgcgcatt tcggccacac
      781 aagcctggtc tcctgtgtaa gcagcccggg ctgcttcttg tgaaaccgag caggggccgg
      841 aggtatattg tccctgcaat ttgttgcagg cggaagctat ccactgtgga gcggcaatcc
      901 agccgatacg ccagccggtc atggcataag ctttggacac gccgttgatg acgattaccc
      961 gttcgctaac ggactcaaac tgggcaatgc tctcgtgttt gcccaggtaa ttgatgtgtt
     1021 cgtaaatctc gtccgaaagt atgtaaacct gaggatgttt ggccaggaca gcggccagag
     1081 cttgcaattc ttctttgctg taaacgctgc cggtagggtt ggaaggagaa cagagaatca
     1141 aggctttggt tttgggggta atggccgctt cgagttgggc ggctgttatt ttgaaatcct
     1201 gctctatgcc ggcggagact attacatttt ttccttctgc caatttgacc atttcaacat
     1261 agctcaccca ataaggagcg ggaacgatga cttcgtcgcc cgggctgata atggaaagca
     1321 gcacgttgca taccgattgc ttggctccat tggaacaaac aatctgatcg acggtataat
     1381 cgaggccgtt ctcttttttc agtttctcca cgatggcctt gcgcagagcc gggtaaccgg
     1441 gaacgggaga ataaaaagaa aagttattgt cgacggcttg tttggctgcc agtttgatgt
     1501 ggtcaggggt aaaaaaatcg ggttcgccga cactcatgtt aataacatca aaacccatag
     1561 ctttgagttc gttgctcttt tgagacatgg caagggtttc ggaaggggcc aggctggcca
     1621 atcggaggga tacggggttc attttagtat ggttaataat tttcggtgca aagctaaact
     1681 aattccattt taattttgcg gattttcatc cattttcagc aaattattta agacttcttc
     1741 ttcaacctcg aaagccgtac cgtggcgtac acccgaggga aagctgatga tttttgaaat
     1801 atcgtcccag acaccttttt ttaagtcttt gcatctggct gccccgtagc gttggttgcg
     1861 gtatttatcc caataaacat aagtgtactt acctatttgc agaggggtgg ggccttcggc
     1921 ccattcgatg ccgctgatag gttcggatac cgttttgggg aaagccgtca gttttttgga
     1981 agctaccaca cgtatgtttt tttcgaccgg ttccagggtt tcgttttt
//
LOCUS       DAOSGL010000364         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_56271_length_2008_cov_3.694316, whole genome shotgun sequence.
ACCESSION   DAOSGL010000364 DAOSGL010000000
VERSION     DAOSGL010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56271_length_2008_cov_3.694316"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1913)
                     /locus_tag="PKY68_09200"
     CDS             complement(45..1913)
                     /locus_tag="PKY68_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH25242.1"
                     /translation="MIKKSFIVLALLCSSLSIFARTEIWVAPGGSDQAPGTKAEPKAS
                     LHSALRQARESRRLSDPLTADGIIIRMQAGTYFLDEPLLIRPEDCGTPESPTRIVAEK
                     SGEVTLSGGVKLGKWSKAGSVPGLPSAAKGKVWVTDQPKVSGRYIDFRQLWVDGRKAV
                     RARDTNDDNFKRILAWDKNTGVLTIPAPGVKSFKTLDRLELQLHQMWAIANLRIKAIE
                     YQNEKALVVFHQPESRIQAEHPWPTPMTAEGRESPFFLCNAIEFLDQPGEWYLDVEDH
                     KLYYWPRPGEDITRAESVVPYLETLIDVQGSLDRPVKHISFEGIGFSYTSWLRPSEQG
                     HVPLQAGMYLLDAYKLRPPGVPGNENRGLENQGWVGRPPAAVQLRGVQNTSFVRCRFE
                     HLASCGIDYISGTLNDSILACVFQDIGGNGIQAGKFSDPGIETHLPYDPVDRREICTK
                     LYIGNNYIHNCSNEDWGCVGICAGYVRDILIEHNELSELSYSGISLGWGWSKTVNCMR
                     NNVVRANYIHHYAKRMYDVAGIYTLSVQSKSLISENVVDSIYSPAYVHDPNHWFYLYT
                     DEGSSFITVKDNWCPAEKFLQNANGPGNTWINNGPMVDEKIRLAAGIEPAYSDIKP"
BASE COUNT          504 a          520 c          453 g          531 t
ORIGIN      
        1 tgccctagcc ttggcaggcg atgaataaaa acgaccggac aatatcaggg ctttatgtcg
       61 ctgtatgccg gctcaatgcc tgctgccaac ctgatttttt cgtcaaccat cggaccgttg
      121 ttaatccagg tattgcccgg gccgttggcg ttctgcagaa atttctcggc cggacaccag
      181 ttatctttta ccgtaataaa agaggagccc tcgtcggtgt aaagataaaa ccagtgattg
      241 ggatcgtgaa cgtaggccgg cgaatagata ctgtcgacca cattttcgct tatcagagat
      301 tttgattgca ccgacagagt gtagataccg gccacatcgt acatacgttt tgcgtaatga
      361 tgaatgtaat tggctctcac cacattgttg cgcatacaat tcacggtttt actccaaccc
      421 caacccaaac tgatacccga ataagacaat tccgatagtt cgttgtgctc aatgagaata
      481 tccctcacat aaccggcgca aatacccaca caaccccagt cttcgttgct gcaattgtga
      541 atgtaattgt ttccgatgta cagtttggta catatctcgc gccgatcgac aggatcgtaa
      601 ggcaagtgtg tttctatgcc cggatcggaa aactttccgg cttgtatgcc gttaccgcca
      661 atatcctgaa agacacaagc taatatacta tcgttcagag ttccgctgat ataatctatc
      721 ccacatgagg ccaggtgctc aaaacggcaa cgcacaaagc tcgtattttg taccccacgc
      781 aattgtacgg cagccggagg tctacccacc caaccctgat tttccaggcc ccggttttcg
      841 ttgcccggta cgccgggagg acgcaattta tacgcatcga gcaggtacat acctgcctgc
      901 aggggaacat gcccctgttc cgagggcctc agccagcttg tgtacgaaaa gccgatacct
      961 tcgaagctta tatgctttac aggacgatcc agactgccct gaacatcgat taaggtttcc
     1021 aaataaggaa ccaccgattc ggcccgggtg atatcttctc ccgggcgagg ccaataataa
     1081 agcttatgat cttccacgtc gaggtaccat tccccgggct gatcgagaaa ctcaatggca
     1141 ttgcacagaa aaaagggaga ttcccggcct tccgcagtca tcggggttgg ccatggatgc
     1201 tcggcctgga tgcgactttc gggttgatga aaaaccacca aagctttttc gttttgatat
     1261 tctatggctt ttatacgcaa attggctatt gcccacatct gatgcagctg caattcgagg
     1321 cgatccaaag ttttaaagga ttttactcca ggagcgggaa tggttaacac ccctgtgttt
     1381 ttgtcccagg ccaatatacg tttaaaattg tcgtcgttgg tatctctggc ccgaacagct
     1441 ttacgaccgt ctacccataa ttgtcgaaaa tcgatgtagc ggcctgaaac tttaggctga
     1501 tctgttaccc agaccttgcc tttggcagcc gagggtaaac ccggaacgct gccggctttg
     1561 ctccatttgc ccagttttac tccgccgctc agtgtcactt cgccgctttt ttcggccact
     1621 atacgggttg gactctcagg agttccgcaa tcttcgggtc ttatcaggag aggttcgtcc
     1681 aaaaaataag ttcctgcttg cattcgaata atgattccgt ccgctgtcaa gggatcggac
     1741 aatcttctac tttccctcgc ctgcctcagg gccgaatgca aactagcctt gggttccgcc
     1801 ttggtacccg gagcctggtc cgagcctccg ggagctaccc aaatttccgt ccgtgcaaaa
     1861 attgacaaag agctacataa cagagctaat acaatgaaag attttttgat cataactatg
     1921 ttgttcctgg acttaataaa tatttctgaa tggttcgcat caaagcgggt ttaaaccata
     1981 ctattcgttt gaaaaacaag ttaaagct
//
LOCUS       DAOSGL010000365         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_56354_length_2006_cov_4.506920, whole genome shotgun sequence.
ACCESSION   DAOSGL010000365 DAOSGL010000000
VERSION     DAOSGL010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56354_length_2006_cov_4.506920"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="PKY68_09205"
     CDS             complement(<1..1959)
                     /locus_tag="PKY68_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25243.1"
                     /translation="MKTPILSIILFVCLGLQAQSRSFEEYMRREQEKMKQFEQEQIEG
                     MERLRKEYAEYVAKRDQEWADYLKKEWENYSAFAAKELPAKPKPKTNPVFDMSSQTES
                     KTISAARAESLKPEPLPAVVEQVRKPAEKAVNFRTANLNFYNRSLAIPYDPAMRAFVL
                     QGISQESIGAFWEKISESNYTPAVESLLEAKKELNINDYAYMLLCQYFSKSLYPANAN
                     AARLMTWFVMLRSGYSLRVGFNDEAIALLVPTQDQVYQKAYLNIDGINYYIFPDLKNN
                     SLYTYDEDYRSGKPVSFGIHSPMNLGGKTAERKVKFEFEGKTYDIKLNYDPDLIDFFQ
                     YYPLLAFETYFNAPMSVQSREALYAALQPVIGEMDEVRAVNFILRFVQTAFAYKTDPE
                     QFGREKYFFAEEVLHYPYCDCEDRSVLFSYLVRELLGLNVVALEYPGHMATAVAFKSP
                     FSGNHLVYNNQRYVVADPTFINAPVGVAMPEYSNATPIIHEIANPQGRAFSLSGLWDD
                     FSEKGCYKGSFRKNAKLLADGSALLTGYFSKTVQLGSFNLPGKGEAHNCFVAKLNPEG
                     KVLWANKLSSSGNAVGMSVDCQASGDIVVAGVFTGELQTGGYKLLAPEGKSDLFMAGY
                     SPEGRLLWLSKAGLDELPQTIYTAFTAVF"
BASE COUNT          477 a          469 c          511 g          549 t
ORIGIN      
        1 gaataccgcc gtaaaagccg tatagatggt ttgaggcagc tcgtccagcc ccgctttgct
       61 cagccagagc agacgccctt cgggggaata accggccata aagaggtctg atttgccttc
      121 gggtgcaagt aatttgtagc ctccggtctg taattctccg gtaaatacac cggctaccac
      181 aatatcgccc gaagcctggc agtcgaccga cattccaaca gcgttgcccg aggaactcag
      241 tttgttggcc cataatactt taccttcggg gttgagtttg gctacaaagc aattatgtgc
      301 ttcgcccttg cccggtaaat taaagcttcc caattgtact gttttggaaa aatagccggt
      361 cagcagggcg gatccatcgg ccaggagttt ggcattcttt cggaaactgc ctttgtagca
      421 acctttttct gaaaaatcat cccaaagtcc cgatagagaa aaggctctac cctgagggtt
      481 ggctatttca tgaataatgg gtgtcgcatt gctgtattcg ggcatagcca cgcctacagg
      541 tgcattgata aaagtgggat ctgccacaac ataacgctgg ttgttataaa cgagatggtt
      601 accgctaaat ggagatttga aggccacggc ggttgccata tgtccgggat attccagggc
      661 gactacgttg agccccagca attcgcgcac caggtaagaa aacaagaccg agcggtcttc
      721 gcaatcgcaa tagggatagt gtaagacttc ttcggcaaag aaatactttt cgcggccgaa
      781 ttgctcgggg tcggttttat aggcaaaagc cgtctgaaca aagcgcagga taaaattaac
      841 cgcccggact tcgtccattt cgccgatcac gggctgcagg gctgcgtaca gagcttccct
      901 ggattgcaca gacatggggg cgttgaaata ggtttcaaaa gcgagcaggg gataatattg
      961 gaaaaagtcg atcagatccg ggtcgtagtt gagcttaatg tcgtaggttt taccttcaaa
     1021 ttcaaattta accttccttt cggccgtttt tccacccagg ttcataggcg aatgaatacc
     1081 aaagctcacc ggtttgccgc tgcggtaatc ttcgtcgtag gtgtacagcg aattgttctt
     1141 caggtcgggg aaaatataat aattgatccc gtcaatgttt aaataggctt tttgatagac
     1201 ttggtcctga gtgggaacaa gcaaggcaat ggcctcatcg ttgaacccca cgcgcaggga
     1261 atagcccgat cgcagcatca caaaccaagt catcagcctg gccgcattgg cgttggccgg
     1321 atagaggctt ttggaaaaat actggcaaag cagcatgtag gcataatcgt tgatgttgag
     1381 ctcctttttt gcctcgagca gactctcgac ggccggagta taattgcttt cggaaatttt
     1441 ctcccagaat gcgcctatac tttcctggct gatgccttgc aacacaaaag ctctcattgc
     1501 cggatcgtag ggaatggcca ggctgcggtt gtagaaattc aggttggcgg ttctgaaatt
     1561 gacggctttc tcggccggtt tgcgaacctg ttccaccacg gcaggcaggg gttccggttt
     1621 tagactttcg gcacgggcag cggaaatagt cttgctttca gtttggctgc tcatatcaaa
     1681 caccgggttg gttttgggtt tgggtttggc gggtaattct ttggcagcaa aggccgagta
     1741 gttttcccat tcttttttca ggtaatcggc ccattcctgg tcacgtttgg ccacatattc
     1801 cgcgtattcc ttgcgcaggc gttccattcc ctcgatttgc tcttgttcaa attgtttcat
     1861 cttttcttgt tcccggcgca tgtattcctc gaaacttcgg gactgtgctt gcaaacccag
     1921 gcagacaaac agtattatgc ttagaatagg tgttttcatg ttcgtatggt gttaaatttg
     1981 acaggatttc ttaaacaatc tttttg
//
LOCUS       DAOSGL010000366         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_57961_length_1970_cov_4.205222, whole genome shotgun sequence.
ACCESSION   DAOSGL010000366 DAOSGL010000000
VERSION     DAOSGL010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57961_length_1970_cov_4.205222"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKY68_09210"
     CDS             <1..288
                     /locus_tag="PKY68_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH25244.1"
                     /translation="RKFFMPSLMQELNGSINTLDGQSKSRLEMKVYNLDQPEELEEFL
                     HPDIQKIKVPRSEELIDYDPQKRTGILMNKLGTSKAIALGAYAFALHELDK"
     gene            complement(311..964)
                     /gene="lipB"
                     /locus_tag="PKY68_09215"
     CDS             complement(311..964)
                     /gene="lipB"
                     /locus_tag="PKY68_09215"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOH25245.1"
                     /translation="MIDIVHWGRIPYSEAWQKQTALFQARIEAKQQGENLLPDLLISC
                     EHNPVYTLGKNGMINNLLLGNNELEQKGVEFFKTDRGGDITYHGPGQIVIYPILDLEH
                     FGLGLKAYINCLEEAVISFLKNRDIQAGRLDGATGVWLQNPARKICAIGVRCSRYITM
                     HGLALNINTDLNYFRFINPCGFVDKGVTSLQAELGGAVQDLNLCRDELMQELKKQFS"
     gene            1114..1734
                     /gene="rsmG"
                     /locus_tag="PKY68_09220"
     CDS             1114..1734
                     /gene="rsmG"
                     /locus_tag="PKY68_09220"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOH25246.1"
                     /translation="MELIKKYFPELNNVQLSQFENLYELYSGWNQKINLISRKDLDRL
                     YERHVLHSLGIARFIRFKPSSRILDVGCGGGFPGIPLGILFPEVEFVLLDSIAKKIRA
                     AEDIAQQSGLSNVQFRIERAENEKGLYDFVVSRAAMSMPDLYKICRKNLARTSRNALP
                     NGILALKGGDLSAELSGFGSKAVVTELSQYFEEEFFATKKVVYLSF"
     gene            complement(1703..>1970)
                     /locus_tag="PKY68_09225"
     CDS             complement(1703..>1970)
                     /locus_tag="PKY68_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH25247.1"
                     /translation="GISATAYSKMENGKTDFSVTRLTQFAQLFKVYLPDFFDENIQVP
                     RYINDSGTVEDYKNKMLEMRAQKELLKEILEYRLLKSSDKPLSL"
BASE COUNT          553 a          455 c          433 g          529 t
ORIGIN      
        1 cggaaatttt ttatgccctc gctgatgcag gaattaaacg gcagcatcaa caccttggac
       61 ggccaatcca aaagccgtct cgagatgaag gtctataatc tggatcaacc ggaggaatta
      121 gaagaatttt tacatcccga catccaaaaa atcaaggttc cccgaagcga ggaactgatc
      181 gattacgatc cgcaaaaacg aacgggaata cttatgaaca aattgggcac gagcaaggcc
      241 atagccttgg gagcctatgc ttttgccctg cacgaattgg ataaataatt gctgtggttt
      301 tcggaccggt tcaagagaat tgtttcttta gttcctgcat taattcgtcc cggcataaat
      361 tcaaatcctg aactgctccg cccaattccg cttgtaggga ggtaacgccc ttatctacaa
      421 aaccgcaggg attgataaac cggaaataat tcaggtcggt attgatgttc aaggccaacc
      481 cgtgcatggt gatgtaacgg ctgcatctga ctccgatggc gcatatttta cgagccgggt
      541 tttgcagcca gactccggtt gcaccatcca ggcgaccggc ctgtatgtct ctgtttttta
      601 ggaaagaaat cacagcttct tccaggcaat taatataggc tttgagtcct aaaccgaaat
      661 gctccagatc caggataggg taaatcacta tttgtcccgg gccgtggtaa gtgatatcac
      721 ctcccctgtc ggtcttaaaa aactccacgc ccttttgttc cagctcgttg ttccccagca
      781 gcagattgtt gatcataccg tttttaccca gagtatagac cggattgtgt tcacaactga
      841 taagcaaatc gggcaacaga ttttcgccct gctgtttagc ttcaattctt gcctgaaaca
      901 atgcggtttg cttttgccaa gcttccgaat agggaattct accccaatgg acgatatcta
      961 tcataaacag taatcttctt gattattgac tgcattgtcg tatttttgca ggctgtattt
     1021 tgaagaacgc atcctgcaga acgtatcctg cggaccataa aaaagtcaaa agtaccgttt
     1081 tttcacaaaa aaccaagaac aaaaggccgc caaatggagc tgataaagaa atatttccct
     1141 gaactgaaca atgttcaact gtcccaattc gaaaacttat acgaactcta ttccggctgg
     1201 aatcaaaaaa tcaacctgat ctcgcgcaaa gacctggaca ggctctacga aaggcatgtg
     1261 ttgcattccc ttggaattgc ccgcttcatt cgcttcaaac caagcagccg catactcgat
     1321 gtgggttgcg gcggaggttt tccgggtatt cccctgggca tactctttcc cgaagtggaa
     1381 tttgtattgc tcgacagcat agccaaaaaa atacgggcag ccgaagacat tgcacaacaa
     1441 agcggtttaa gcaatgtgca attcagaata gaacgggccg aaaacgaaaa gggactctac
     1501 gattttgtgg ttagccgggc cgcaatgtcg atgcctgatt tgtacaagat ttgccgtaaa
     1561 aaccttgccc gaacatcgcg taatgcgttg cctaacggaa tactggcttt aaaaggcggc
     1621 gacctgagcg cagaattgtc gggttttggc tccaaagccg tggtaacgga actatcgcaa
     1681 tacttcgaag aagaattctt tgctacaaag aaagtggttt atctgagctt ttgagtaaac
     1741 gatattccag aatttccttg agtaattctt tttgtgccct catttccaac attttgttct
     1801 tataatcttc cacagtgccg gagtcattga tgtatcgcgg cacctgaata ttctcgtcga
     1861 aaaaatcggg cagatacact ttgaacaatt gtgcaaattg ggttaatctg gtgacggaaa
     1921 aatccgtctt cccgttttcc attttggaat aagcggtagc tgaaataccg
//
LOCUS       DAOSGL010000367         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_59331_length_1940_cov_3.392042, whole genome shotgun sequence.
ACCESSION   DAOSGL010000367 DAOSGL010000000
VERSION     DAOSGL010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59331_length_1940_cov_3.392042"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1459
                     /locus_tag="PKY68_09230"
     CDS             164..1459
                     /locus_tag="PKY68_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25248.1"
                     /translation="MKRFLVLIYGILLSLPFTAQNTTQGVTQNSSKSEIPTKTWDLYG
                     FIRSDFFVQNRNTRAVVLDLFPLYPMPKYPNEVGDDLNNSPSAGLSSITTRIGFKFNS
                     AGIFGAQKYTGIIETDFGGSSDYVLVRIRKAYSQLDWEKSRLILGQTWHPLFIEELMP
                     NVVSINTGAPFQALNRSPQIRYNYRGQDFNLTAAAIYQVMYTSSGPRGKSNQYQKDAV
                     IPNLFFGVEYNTGKLITGIGGDFKAIRPEKTWIDNSNIERINTRLLNTYSGIAYLKFA
                     HNRWSIISKTLLGQNLSDHSIIGGYIIDNEHNYLSLNTLASLAQVQYGQIHKLGLFGG
                     YSAILGSTASTNENIYNFYGFGAELIPSSNQYQYIRDMFRVMLTYTYNPKNWRMGVEL
                     EYNGVNWGSLNKNPGNQFRISADDYTAAYRMHAVIMYLF"
     gene            complement(1652..>1940)
                     /locus_tag="PKY68_09235"
     CDS             complement(1652..>1940)
                     /locus_tag="PKY68_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25249.1"
                     /translation="PVFEPALGNVGRIYYNKAVEELDRVNAIRDDRVYRREKAKMKEV
                     FEKPLPYMEKAHELNPEERDYIIALRGIYYNLGKGFEKKYEEMDALMKQTR"
BASE COUNT          592 a          408 c          379 g          561 t
ORIGIN      
        1 cggcatagat ctatgcggtt ccctctataa aaaagtagat ctggaagtat ttattgaaaa
       61 aacacaagca cttagaatcg gtgaatttta ccgcgccgaa atttatagtg caggtatata
      121 cgatttatac ggtaatattc gatcttagtc aactccttta aacatgaaaa gatttttggt
      181 attgatttat ggtattttac tcagtttacc tttcacggct caaaatacaa cccagggcgt
      241 aactcaaaac tcttcaaaaa gtgaaattcc aacaaaaacc tgggatttgt acggctttat
      301 acgcagcgat ttttttgtac aaaaccggaa tacccgtgcc gttgttctcg atttatttcc
      361 gctttatcca atgcctaaat acccgaacga agtaggagac gacctcaaca actccccttc
      421 ggcaggtttg tcgagcataa ctacccgtat aggattcaaa ttcaactctg caggtatatt
      481 tggtgcccaa aaatatacag gcatcatcga aaccgacttt ggaggcagct ccgactatgt
      541 attagtgcgt atacgcaagg cttacagtca gttggattgg gaaaaaagcc gcctgatact
      601 gggtcagacc tggcatccgc tgtttattga agagttaatg cccaacgtgg tatccataaa
      661 taccggagcg ccttttcagg ctttaaaccg cagcccacaa atacgctata attatagagg
      721 tcaggatttt aacctgacag cagcagccat ttatcaggta atgtatacct cttcgggccc
      781 tagggggaaa agcaatcaat atcaaaaaga tgccgtcatt cccaatttgt tttttggagt
      841 cgaatacaac accggcaagc tgattacggg tataggagga gactttaagg ccatacgtcc
      901 ggaaaaaacc tggattgaca actcaaacat tgaacgcata aacaccagat tattaaacac
      961 atactcgggt atagcctacc tgaaatttgc ccataataga tggtcaatca taagcaagac
     1021 cctgcttgga caaaatttga gtgatcacag cataatcggg ggatacatta ttgataatga
     1081 acataattac ttgtctctaa acactttggc cagtttagca caagtacaat acggccagat
     1141 acataaattg ggccttttcg gaggttattc agccattttg ggcagtacag cttcgacaaa
     1201 tgaaaacatt tacaattttt acggttttgg cgcggaatta attccctcca gcaatcaata
     1261 ccagtacatt cgggatatgt ttcgcgtaat gcttacttac acctataatc ccaaaaattg
     1321 gcggatggga gttgaattgg aatacaacgg agtcaactgg gggagcctga ataaaaaccc
     1381 cggcaatcaa tttagaattt ctgccgatga ttatacggcc gcttaccgga tgcacgctgt
     1441 tataatgtac ttgttttaaa cccgtctact gtgccggatg agagaataca aaaaatcaag
     1501 acaccgtatt aactgtcgac tatgaatatc taaaaaaagc tgcctcctaa aagagacagc
     1561 ttttttcgta gaaggggtaa aagcttttta ttttgagaac agcccttttc tattcaacat
     1621 aatattaatt atgggctttg agtgcgtgaa tttaacgcgt ttgtttcata agagcgtcca
     1681 tctcttcgta tttcttttca aagcctttgc ctaaattata ataaatacct cttaaagcaa
     1741 tgatgtaatc tctttcctct ggattaagct cgtgtgcttt ctccatataa ggcaaaggtt
     1801 tttcaaatac ctctttcatt ttggcttttt cgcggcgata aacccggtcg tctctaatgg
     1861 cgtttacacg gtcaagttct tcgactgctt tgttgtaata aatacggccg acattcccca
     1921 aagccggttc aaaaacgggg
//
LOCUS       DAOSGL010000368         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_59434_length_1938_cov_3.161976, whole genome shotgun sequence.
ACCESSION   DAOSGL010000368 DAOSGL010000000
VERSION     DAOSGL010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59434_length_1938_cov_3.161976"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKY68_09240"
     CDS             <1..544
                     /locus_tag="PKY68_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOH25250.1"
                     /translation="QEALALRGTHNLYNTMASAISANIAHIRKEEIRTALSNFKGVEH
                     RLEKVAGVAGVEYINDSKATNVNSCWYALESVNTPVVLIMGGTDKGNDYGEIEALVRQ
                     KVKAIVCLGVDNTKLHDFFRDKVDEIRDARSMKEAVDLASNLATKGDTVLLSPCCASF
                     DLFENYEDRGRQFKDCVRNL"
     gene            548..1774
                     /locus_tag="PKY68_09245"
     CDS             548..1774
                     /locus_tag="PKY68_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOH25251.1"
                     /translation="MELIQSVFKGDKVIWMIFLFLCIISLVEVFSAIGTLAYARMNPF
                     EPIIRHGLYLLIGVAMVVAFHNFHYKYTALLVFPLLLLSVIMLILTPIIGVRLNEAAR
                     WIEIFGIQIQPSELAKLSMVLFSAWFLGRAQGSKEAMDKAFWVVLIVTAALAFLIVFE
                     NLSTAILLCLFVFLMMVVAGVDWKKLLILFLLVASAAALLLFLLIKIERLDGVPRWET
                     WHNRLVTEQLDVRDEGFKITDENRQVSHAKIAISGGKFLGVLPGNSSQRDFLPQAYSD
                     FIFAIIIEDLGWLGLFGVPFLYLFLLFRALKIAKRCTKSVPMLLVLGSALIISMQAFV
                     NMAVAVNWFPVTGQPLPLISRGGSSGVITCLYFAIILSVSNFATQKPIPEEENLISNE
                     TEDPDYEQQEEIEAAD"
     gene            1740..>1938
                     /locus_tag="PKY68_09250"
     CDS             1740..>1938
                     /locus_tag="PKY68_09250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH25252.1"
                     /translation="MNSRKKSKQQTEETRKPRFIISGGGTGGHIFPAISIANALRQKC
                     PEADILFVGAENRMEMEKVPAA"
BASE COUNT          474 a          403 c          514 g          547 t
ORIGIN      
        1 acaggaagct ttggccttgc gaggaaccca taacctttac aacactatgg cgtcggctat
       61 ttcggccaac atagcgcata ttcgcaaaga agaaatacgc acggccctga gtaatttcaa
      121 gggtgtggaa catcgtttgg aaaaagtggc cggggtggcc ggggtggaat acatcaacga
      181 ctccaaagcc accaacgtaa attcctgctg gtacgccctc gaatcggtca atacaccggt
      241 agtgttgata atgggaggta ccgacaaagg aaacgactac ggcgaaattg aagctttggt
      301 caggcaaaag gtgaaagcca tcgtttgcct gggtgttgac aatactaagc tgcacgattt
      361 cttcagggac aaagtagacg aaattcgcga tgcacggtcg atgaaagagg cagtggacct
      421 ggcaagcaat ctggcaacca aaggcgatac cgtcttgttg tcgccttgct gcgccagttt
      481 cgaccttttc gaaaactacg aagaccgcgg tcgtcaattc aaggactgcg ttcgcaatct
      541 ataagaaatg gaactgatac aaagtgtgtt caaaggtgac aaagtgatct ggatgatctt
      601 tttgttttta tgcataattt cgttggtgga ggtttttagc gccatcggga ccctcgctta
      661 tgcccggatg aatcctttcg agcctatcat aagacacggc ttgtatttat tgataggggt
      721 tgccatggtg gtggcttttc ataacttcca ttacaagtat accgctttgc tggtgttccc
      781 cctgctattg ttgtcggtta ttatgcttat tctgactccc ataataggtg tcaggctaaa
      841 cgaagcagcg cgttggatag agattttcgg catacagatt caaccctcgg aactggccaa
      901 attgtcgatg gtgttgtttt ctgcctggtt tctgggcagg gctcagggct ctaaagaggc
      961 catggataag gcattttggg tggtgcttat tgtgacggcc gccttggctt ttttgattgt
     1021 gtttgagaat ttgtccactg ctattttgct ctgcctcttt gtttttctta tgatggtggt
     1081 ggccggggtg gactggaaaa aactcctgat tttgttttta ctggtggctt cggcagccgc
     1141 tttgctgttg tttttgttga tcaagataga gcgcctcgac ggggtgcccc gctgggaaac
     1201 ctggcacaat agattggtga ccgaacagct ggatgtcagg gatgaaggat ttaaaattac
     1261 ggacgagaac cggcaggttt cacatgccaa aatagctatt tccgggggta agtttttggg
     1321 cgttttaccc ggcaacagca gccagcgtga ttttttgccc caagcctatt ccgattttat
     1381 ttttgccatt atcatagagg atttgggttg gttgggtttg tttggggtgc ctttcctgta
     1441 tttgtttttg ttgttcaggg ctttaaaaat cgccaaacgt tgtacaaaat cagtgcctat
     1501 gctcctggtg cttggttcgg cactgattat atctatgcag gcttttgtca acatggcagt
     1561 ggcggtaaac tggttcccgg ttaccggaca acctttgcct ttgataagca gaggaggcag
     1621 ttcgggagtc attacttgtt tgtattttgc gattatactg agtgttagca attttgctac
     1681 acaaaaacct atacccgaag aggaaaactt aatatcgaac gaaacggaag atccggatta
     1741 tgaacagcag gaagaaatcg aagcagcaga ctgaagaaac gcgtaaaccg cgttttatta
     1801 tttcgggagg aggtaccgga gggcatattt ttccggccat ttccatagcc aacgccctta
     1861 ggcaaaagtg ccccgaggcc gacatacttt ttgtcggcgc cgaaaaccgc atggaaatgg
     1921 aaaaagtgcc ggcagcag
//
LOCUS       DAOSGL010000369         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_59698_length_1932_cov_3.820991, whole genome shotgun sequence.
ACCESSION   DAOSGL010000369 DAOSGL010000000
VERSION     DAOSGL010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59698_length_1932_cov_3.820991"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..327)
                     /locus_tag="PKY68_09255"
     CDS             complement(19..327)
                     /locus_tag="PKY68_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOH25253.1"
                     /translation="MKIAITSEGDHLQALVDARFGRCAFFAVYDTETQQTTFHANPAK
                     ELAHGAGPAAVQFVARLGVSRVLSGEFGTKVVPLLQELNIDRQQIQPERLDKVIANLK
                     "
     gene            complement(354..1190)
                     /locus_tag="PKY68_09260"
     CDS             complement(354..1190)
                     /locus_tag="PKY68_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH25254.1"
                     /translation="MKAVVLSDNRDSSVLPETEHGLSVYLDRGTYKVLLDTGASDLCI
                     RNAGSLGIGLEDVDCIFISHGHADHMGGLASLLKINTKAKVIISPQALSQRFFSNRKS
                     VREIGVQLLTDQYPGRFVFVREDAFIRPDFQVVKPKKWGFPLPKGNQHLFKDSGRGQE
                     PDDFDHEMMACFGSEELFVYCGCAHHGLPNILQSINTKINKPIRAMLGGFHLLDSDEQ
                     HTYETEKELSELAEYLKTTQPNTRFYTGHCTGSGAYFFLKQSLGADLELFHTGYEMEL
                     SI"
     gene            complement(1193..1603)
                     /locus_tag="PKY68_09265"
     CDS             complement(1193..1603)
                     /locus_tag="PKY68_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOH25255.1"
                     /translation="MPRPKLNRRIAAPPLMMGYKPFGIPRKVLSELYLHFDEFEAIRL
                     LDYLGMTQAEAADKMNVSRPTLTRIYEKARRTIARAFVEGKMIMIEGGQVAFEGEWYR
                     CKRCHRLIKGRENHVPCKDCDSYSDEELYRVNEP"
BASE COUNT          521 a          449 c          413 g          549 t
ORIGIN      
        1 tggggtttac ttctgttttt acttcagatt agcaatgact ttatccagtc tttcgggctg
       61 tatttgctgc ctgtcgatgt ttaactcctg caacagaggt acaactttgg ttccgaactc
      121 cccggaaagc acacggctta cccccagacg ggccacaaac tgaaccgctg ccggaccggc
      181 accatgggca agttctttag ccggattggc atgaaaagtg gtttgttggg tttcggtgtc
      241 gtacacagca aaaaaggcgc agcgcccaaa acgagcgtcg actaaggctt gtaaatgatc
      301 gccttcggat gtaatagcaa ttttcatagt tctgtaaata ttaaaatgtt aatttaaata
      361 gatagttcca tttcatagcc ggtgtggaaa agttccaagt ctgcacccag gctttgtttc
      421 aaaaaaaaat aagcccccga cccggtgcaa tgacccgtgt aaaaacgagt gtttggctgg
      481 gtggttttca aatactccgc caattcggac agctcttttt ctgtttcgta ggtatgctgt
      541 tcatcgctgt ccaataagtg aaaaccgcca agcatggccc ttatgggctt attaatctta
      601 gtgttgatgc tttgtaaaat attcggcaaa ccgtgatgag cgcaaccaca atacacaaaa
      661 agctcttcgc tgccgaaaca agccatcatt tcgtgatcga aatcgtcggg ctcttgtcct
      721 cttccgctgt ctttaaataa atgttgattg ccctttggca aagggaaacc ccattttttt
      781 ggctttacta cctgaaaatc gggccttata aaagcatctt cgcgcacaaa aacaaaacgg
      841 ccgggatact gatcagtcaa gagttgaaca ccgatttccc ttacactttt ccgattggaa
      901 aaaaagcgtt gagagagcgc ctgtgggctt ataataactt tggctttcgt gttgattttc
      961 aacaaagagg ctaaaccgcc catatggtcg gcgtgtccgt gagaaataaa aatacaatcg
     1021 acatcctcca ggccgattcc caaactcccg gcgttacgaa tacataaatc ggaagctccg
     1081 gtatcgagca acactttgta agttccccta tccagatata ccgacaatcc gtgttccgtt
     1141 tcgggcagaa cagaagaatc gcgattatcg gacaggacta cggctttcat aatcagggtt
     1201 catttacacg atagagttct tcgtcggaat aactgtcgca gtctttgcaa gggacatgat
     1261 tctcccggcc tttaatcagg cgatggcaac gcttgcatct gtaccattcc ccttcgaatg
     1321 ccacctggcc gccttcaatc attatcattt tcccctccac aaaagcccgg gcaatggttc
     1381 tgcgggcttt ttcgtagata cgggtcagtg taggccggga tacattcatt ttatcggctg
     1441 cttccgcctg ggtcattccc aggtaatcga gcagccttat tgcttcaaat tcatcaaaat
     1501 gcaaataaag ctcgctcaag acttttcgcg gaattccaaa aggtttgtag cccatcatta
     1561 aaggcggggc agcaattcta cgatttagtt taggtcgggg catggtttta taataataga
     1621 agcagtttta tataatgaga gcagttccac ataatagaag tggtcttgta cgactatacg
     1681 tggtttcaca tattgaacgg caaagataag aggaattttg aacttatgtt catttcttcc
     1741 cctatttatt tgttaaattc ccttaggtaa acactaaagc tgagaactca attcatcgta
     1801 agcaatcagc gcatcgtaat ctgctcctac aggccggtaa tacacaaaaa tctggtattc
     1861 gttttcagtt tcccaaaaac tcccttccac cggaaaagtt gtacccgggt ttttctttgc
     1921 ggaaatggtt ga
//
LOCUS       DAOSGL010000370         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_62368_length_1879_cov_2.238487, whole genome shotgun sequence.
ACCESSION   DAOSGL010000370 DAOSGL010000000
VERSION     DAOSGL010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62368_length_1879_cov_2.238487"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKY68_09270"
     CDS             <1..826
                     /locus_tag="PKY68_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOH25256.1"
                     /translation="RSEKDPYTLMRMIDVNIFNTVKTLKQAGEKGAKKYFCVSTDKAA
                     NPANMMGATKRIMEMFLMQHSRDICISTARFANVAFSDGSLLQSFEQRLQKRQPIVAP
                     NDVKRYFVTARESGELCLLSTLLGGNRDIFFPKLSQDLHLQKFSDIAVRYLQARGYEP
                     FLCRSEQEARELMHSLPDEGRWPCLFTASDTTGEKEYEEFYTGNELPDMERFRSLGIV
                     RNPPDCDPQKTARFVRVVEGMKASGSWSKEELVALFFEMIPDFAYIEKGKYLDSKM"
     gene            958..>1879
                     /locus_tag="PKY68_09275"
     CDS             958..>1879
                     /locus_tag="PKY68_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HOH25257.1"
                     /translation="MRQAFGQPRGTIPLHAPVFGGNEKKYLGECIDSTYVSSVGRFVD
                     RFEEEMARFTGAAKAVVCVNGTNALHLALHLAGLERDEEVITQPLTFVATVNAIAYGA
                     AHPVFVDVDRDTMGLSPAALRAWLEQNVRIEQGQGKDPKPGAYNVKTGRRIAAVVPMH
                     TFGHPCRIDETADLCKQYHIELIEDAAESLGSYYKGQHTGTFGKMGVLSFNGNKILTT
                     GGGGMLLFRDEALAKRAKHISTQAKVTHPWAFVHDEIGYNYRMPNINAALGLAQLEQL
                     ETFLAAKRKLAGEYKQFFEGKVGIRYVEEPR"
BASE COUNT          507 a          487 c          496 g          389 t
ORIGIN      
        1 acgcagcgaa aaagatccct acaccctgat gcggatgatc gacgtaaaca ttttcaacac
       61 cgtcaaaacc ctgaagcagg ccggagaaaa gggagcgaaa aaatactttt gcgtatccac
      121 cgacaaagcg gccaatccgg ccaatatgat gggcgccacc aagcgcatta tggaaatgtt
      181 tctgatgcaa cacagccgcg atatctgcat ttcgacggcc cgttttgcca acgtggcttt
      241 ttcggacgga tcgttgctgc agagcttcga acaacgtttg cagaaacgcc agcccattgt
      301 ggctcccaac gatgtgaaac gctattttgt cacagcccgt gaatcgggcg aattatgtct
      361 gctgtccacc ctgctgggcg ggaatcgcga catctttttt cccaaactca gccaagactt
      421 gcacctgcaa aaattttccg atattgccgt acgctatttg caggccagag gttacgagcc
      481 ctttttgtgc cgttccgaac aagaagcccg cgagttgatg cacagcctgc ccgacgaggg
      541 ccgctggcct tgtttgttta ccgccagcga taccaccggc gaaaaggaat acgaagaatt
      601 ttataccggc aacgaactac ccgatatgga gcgatttcgg agcctgggga tcgtcaggaa
      661 ccctccggac tgtgatccgc aaaaaacagc ccgctttgtc cgcgtcgtcg agggaatgaa
      721 agcatcgggg agctggagca aagaagaatt ggtcgcgctg ttttttgaaa tgattcccga
      781 ttttgcctac atcgaaaaag gcaaatacct cgattcaaaa atgtaatagg aatcagcaga
      841 aaataattga ttaaaaatag ataatcagct aattataaaa ccgtaagacc ggaaaaaaca
      901 tacaaataag tgcagaataa ttcagaaaac aagctttttg ccgaaacgct gcagtttatc
      961 aggcaggcat tcgggcagcc ccgggggacg atacctctgc atgcacctgt gtttggaggg
     1021 aacgaaaaaa aatacctcgg ggaatgcatc gactccacct atgtgtcgag tgtgggcagg
     1081 tttgtcgacc gtttcgaaga agaaatggcc cgctttaccg gagcggccaa agcggtggtt
     1141 tgcgtcaacg gaaccaatgc gctgcatctg gctctgcatc tggccgggct ggaacgcgac
     1201 gaagaggtga tcactcagcc actgactttt gtggccaccg tcaatgccat tgcctatggc
     1261 gctgcccatc ctgtgtttgt cgatgtagac cgcgatacga tggggctttc gccggcagct
     1321 ctgcgagcct ggctcgaaca aaacgtccgg attgaacaag ggcagggcaa ggacccgaag
     1381 ccgggggcgt acaatgtaaa gaccggacgg cgcatagccg cggttgtgcc catgcacact
     1441 tttggccatc cctgccgtat agacgaaaca gccgatcttt gcaaacagta tcacattgaa
     1501 ctgatcgaag acgcggccga aagcctgggc agttattaca aggggcagca caccggcacc
     1561 ttcggcaaaa tgggcgtact tagtttcaac ggcaacaaaa tactcaccac cggcggggga
     1621 ggcatgttgc tttttcggga cgaagccctg gccaaaagag ccaaacacat cagcacccag
     1681 gccaaagtga cacatccctg ggcctttgtg cacgacgaaa tcggttacaa ctaccgcatg
     1741 cccaacatca atgcggcttt ggggctggcc cagctggaac agctcgaaac ttttctggca
     1801 gctaaacgca aactggccgg ggagtataag cagtttttcg aaggcaaagt gggcatccgt
     1861 tatgtggaag agccccgag
//
LOCUS       DAOSGL010000371         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_63405_length_1859_cov_3.623060, whole genome shotgun sequence.
ACCESSION   DAOSGL010000371 DAOSGL010000000
VERSION     DAOSGL010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63405_length_1859_cov_3.623060"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PKY68_09280"
     CDS             complement(<1..1371)
                     /locus_tag="PKY68_09280"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOH25258.1"
                     /translation="MFAYIILFVSSMTINPFSIAYIASALITLGLMVFLFVKRKESLS
                     TYVYMLLTCTAVWSFFYGLELGSSNSEDIRFYVQIEYAGICLLPVFYLFYILEYTGRE
                     KHLNTSLYPLFFIIPLVTISMLMSNDVHHLFYAQSSFKTAGRYYYHALVPGPFYHLHV
                     VYSYGLIVLGIVLLLKSYRGQSKMQRNNILILLSGTVIPFLTSIAYILGFKYAGFIDF
                     TPLGFLVMSIIVAYAASTNRLFEMTPLAMSYLYEIIPDAIFVLDRDSAIINTNPSAQK
                     LLKSGIMEECPESEKCLHFEEFSDVEEIKYKEIKAGDKHFFITKKAVVGGNNRQIGLM
                     LIVKDVTEVKNLEHMQEILMSIALNFINTPLDNYEKTVKASLEQMGRYVNAERVCIAS
                     FNYGKGVVDLIHEWRKPGVDSIIDEIRIISLEDIKELIEIHKRRESFFVEDAKVLIEG
                     TPLYELL"
     gene            1445..>1859
                     /gene="pcm"
                     /locus_tag="PKY68_09285"
     CDS             1445..>1859
                     /gene="pcm"
                     /locus_tag="PKY68_09285"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="catalyzes the methyl esterification of
                     L-isoaspartyl residues that are formed in damaged
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOH25259.1"
                     /translation="MNKLFLREINDSETLRAMEAVDRKFFVPEKLQSHAYSDTALPIG
                     LGQTISQPYIVAYMTASVKAGDCGKILEIGTGSGYQTAILAELAREVYTIEIIPELGI
                     KAKSVLDTLGYKNIRYKTADGYRGWQEMAPFDAIVV"
BASE COUNT          590 a          398 c          366 g          505 t
ORIGIN      
        1 gagcaattca tacagagggg tcccttcgat caataccttg gcatcttcta caaaaaaact
       61 ctcccgcctt ttatgaatct caataagttc tttgatgtcc tcaagactta ttatccggat
      121 ctcatcaata atagaatcga ctcccggttt acgccattca tggataagat caacaacacc
      181 cttaccgtag ttaaaacttg ctatacaaac cctttcagca ttcacataac gccccatttg
      241 ttccaacgat gctttaaccg tcttttcgta attatccaga ggggtattga taaaattcag
      301 agcaatcgac atcaggattt cctgcatatg ctcgagattt tttacttccg taacatcctt
      361 cactatcaac atcaagccga tttgccggtt gtttcctcct acaactgcct ttttggtaat
      421 aaaaaaatgc ttatcgcccg cttttatttc tttgtatttt atttcctcca catcggagaa
      481 ttcttcaaaa tgcaaacatt tttcgctttc ggggcattcc tccatgatgc cggactttaa
      541 taatttttgg gcgctgggat tggtatttat tatagctgaa tcacggtcca acacaaagat
      601 ggcgtccgga atgatctcgt ataaataact catagccaga ggagtcattt caaatagtct
      661 attggtagag gcagcatagg ccactattat agacattacg agaaaaccca ggggggtaaa
      721 atcgataaaa cccgcgtatt tgaaaccaag tatataggcg atactggtaa ggaaaggaat
      781 tactgtgccg gagagtaaaa tcaaaatatt attgcgttgc attttactct gtcccctgta
      841 tgacttaagc aacaatacta tacctaaaac tatcaggcca taggaataaa ccacatgaag
      901 gtggtaaaaa ggtccgggaa ctaatgcatg gtaatagtag cgacctgcgg ttttgaacga
      961 agactgagca taaaaaagat gatgaacatc gttgctcatc aacatggaaa tagtaacaag
     1021 cggtattata aaaaataagg ggtagagcga cgtgttcagg tgtttttccc tcccggtgta
     1081 ttcaagtata taaaacaggt aaaaaaccgg caacaaacag atgccggcat actctatctg
     1141 cacatagaac ctaatgtctt cgctgttgga cgaacccaat tccaggccgt aaaaaaacga
     1201 ccatacggca gtacaggtca acaacatgta aacataagtg ctaaggctct ctttcctttt
     1261 aacgaaaaga aaaaccatca ggcccaaagt tataagagcc gaggcgatat aggcgatact
     1321 aaaaggattg atggtcatcg acgaaacaaa taaaattatg taagcaaaca tacaaatata
     1381 gtatgttttt gacttttgtc ttttattttt ttaattttga ttgatattta attctttaag
     1441 agtcatgaat aagctctttt taagggagat aaacgattcc gaaaccctcc gggcaatgga
     1501 ggctgtcgac agaaaattct ttgttccgga aaaactgcaa tcgcatgcct acagcgatac
     1561 agccctaccc atcggcctgg gtcagacgat atctcaaccc tatatcgtag cctatatgac
     1621 agcctcggtc aaagcaggcg attgcggcaa aatacttgag ataggcaccg gatcgggcta
     1681 ccagacagct atcctggccg aactggccag ggaagtatat accatcgaaa ttattccgga
     1741 actgggaatc aaagccaaaa gcgtcttgga cacactgggt tataaaaaca ttcgttacaa
     1801 aacggcagac ggttaccgtg gctggcagga aatggcgcct ttcgatgcca tagtcgtta
//
LOCUS       DAOSGL010000372         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_64252_length_1842_cov_4.095132, whole genome shotgun sequence.
ACCESSION   DAOSGL010000372 DAOSGL010000000
VERSION     DAOSGL010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64252_length_1842_cov_4.095132"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKY68_09290"
     CDS             <1..322
                     /locus_tag="PKY68_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HOH25260.1"
                     /translation="GKEVFHEGPFEEGTNNIGEFLAIVHALALLDKRNICLPIYSDSA
                     NALLWVKKKKCGTKLMPSPKNTELFQLIGRAEQWLLNHDRDFPVYKWDTKAWGEIPAD
                     FGRK"
     gene            complement(327..1400)
                     /locus_tag="PKY68_09295"
     CDS             complement(327..1400)
                     /locus_tag="PKY68_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25261.1"
                     /translation="MRRVALWLTSVFLLLGTGSKAQELLYDLELLSYFDNREYKVDFQ
                     RPQTILGMRIAPELGLGTADEKAGSHKLMLGLNYIQPLGAGIKEALVVPTVYYRYVHN
                     RFRVNLGAVPYSYLTESLPDYLMYDSLAYVHPNMQGGLFQYVAPRGYAEFLCDWRSAQ
                     SLERYEAFRLIMQARYQRGILFGGGRAQLNHLANRSRPAPRTGVSDDLIVHPYLGLDF
                     SRKLPLDSLSLQAGYLFGYQRDRSVDQETYLPRAFYLELFARWKRIGLKNVLYAGDNL
                     LPLYPVKGNLLNQGDPFYQAGFYNRTDAFVYLISTKRVNCYLSFNFHYTRQGGLNHQQ
                     QLVLRFNPGNILPMEKSMSVLGK"
     gene            complement(1420..>1842)
                     /locus_tag="PKY68_09300"
     CDS             complement(1420..>1842)
                     /locus_tag="PKY68_09300"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOH25262.1"
                     /translation="GRGSVIVQTSDPQHPVIQHVLNNDYRAFFQKECEERSKYAYPPF
                     SRLIEVIFRHKDESLLEQASVFFASLLPRDMESSVFGPMPALVARVKGLYLQKIILKI
                     GPDISLKAAKDYLCRARDRFIKDKVYRQVQIYFDVDPV"
BASE COUNT          529 a          470 c          418 g          425 t
ORIGIN      
        1 cggcaaggag gtgtttcacg aaggcccttt tgaagaaggc accaacaaca taggtgaatt
       61 tttggccata gtgcatgcgc tggctttgct ggacaaacgc aacatttgcc tgcccatcta
      121 ttccgacagc gccaatgccc tgctgtgggt taaaaagaaa aaatgcggaa ccaaactcat
      181 gccaagcccc aaaaacacgg aactttttca actgatcggc cgggccgaac aatggctgct
      241 caatcatgac cgggattttc ctgtttataa atgggatacg aaagcctggg gtgagatccc
      301 cgcagacttt ggccgaaaat aaactcttac ttacccaata ccgacataga cttttccatc
      361 ggtaggatat ttccgggatt aaaccttaac accaactgct gttggtgatt caaacctccc
      421 tgccgggtgt aatgaaaatt aaacgacaaa taacaattga cccttttggt cgatatcagg
      481 taaacaaagg catcggtgcg gttatagaat cctgcctggt aaaaaggatc tccctgattg
      541 agcaaattcc ccttgacagg atagagggga agcaggttgt cgcctgcgta caagacgttc
      601 ttcagaccaa tgcgcttcca gcgggcaaac aattccaaat aaaaagcccg gggcaggtat
      661 gtttcctgat ctaccgagcg gtctcgctgg tatccaaaca aatagcccgc ctgaagggaa
      721 agagaatcca gaggcagttt ccgtgaaaag tcaagcccca gatagggatg cacgatcaaa
      781 tcatcgctta ctcccgttcg cggagccggt ctggaccggt tggccaaatg attgagctga
      841 gcccggcccc ctccaaacaa gatacctctc tgataacggg cctgcataat cagcctgaag
      901 gcttcgtaac gctcaagact ctgagcgctg cgccaatcgc ataaaaactc cgcgtagcct
      961 cttggtgcca catattgaaa caggccaccc tgcatattag gatgcacata agccaaagaa
     1021 tcgtacatta aataatcggg cagactctct gttaaatagc tataaggaac agcacccaga
     1081 ttcaccctga acctgttgtg cacataacgg taatatacgg taggtacgac caaagcttct
     1141 ttgatgccgg cgcccaaagg ttgtatgtaa ttcaaaccca gcatcagttt atgactgccg
     1201 gccttttcat ctgccgttcc caggcccagt tccggtgcaa tgcgcatacc gagtatggtt
     1261 tgcgggcgct gaaaatcgac cttgtattcc cggttatcaa agtaagacag cagctcgaga
     1321 tcgtacaaga gttcctgtgc ctttgaaccg gtgcccaaaa gcaaaaaaac cgaagtcaac
     1381 caaagagcaa ctcttctcat gttattatat cttagacact tataccggat caacatcaaa
     1441 atagatttgc acctgacgat acactttatc tttaataaac cggtccctgg cccgacaaag
     1501 atagtctttg gcagccttca acgaaatatc cggcccgatt ttaagaatga tcttttgcag
     1561 atagagccct tttacccgcg ctacgagagc cggcataggg ccgaaaaccg aactctccat
     1621 atcccgaggc agaagagagg caaaaaaaac ggaggcctgc tcaagcaaac tctcatcctt
     1681 atgcctgaat atcacctcaa taagacggct aaagggcgga taggcatatt tggaacgttc
     1741 ctcacattct ttttggaaga aggcccggta gtcgttgttc aaaacgtgtt gtattacagg
     1801 gtgctgagga tcggaggtct ggacgattac actgcctctc cc
//
LOCUS       DAOSGL010000373         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_65129_length_1826_cov_4.211180, whole genome shotgun sequence.
ACCESSION   DAOSGL010000373 DAOSGL010000000
VERSION     DAOSGL010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65129_length_1826_cov_4.211180"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PKY68_09305"
     CDS             complement(<1..1112)
                     /locus_tag="PKY68_09305"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOH25263.1"
                     /translation="MRFRCLLFFLVLLMPGWLSLVARDRTVKLTGFVMDANKRAIELV
                     NIFEQHSQKGTASDQSGYYELELPWQDTLILYYSCLSYQTAVRIVPVEVNQLVLNVIL
                     TASSKTLDEAVVTAQQRRTGSLEKVDISGIRLLPDPTGGSIEALLVTFAGVSSNNELS
                     SQYSVRGGNYDENLVYVNGMEVYRPLLIRSGQQEGLSFVNPSMTDEVKFSAGGFEAMY
                     GDKMASVLDIRYKKPEEFEGSAAISLLGANVFVGQASKNGKFRQIHGMRYKTNAYLLG
                     ALDTKGEYRPSFLDYQTFMSYTLASKWELSFLGNFSQNNYNFIPETRKTKYGTFNNKY
                     EFYVYFEGKEKDLFRTAFGALNLEYKPRRNLNLGLST"
     gene            1191..1724
                     /locus_tag="PKY68_09310"
     CDS             1191..1724
                     /locus_tag="PKY68_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH25264.1"
                     /translation="MDNFIDLVRQRRSTRKFMETSISPDLVAELMRAALMSPSSKRSN
                     PWEFVLVDDAEMLKKLSLSKKHGSALIEGASLGIVVIADKTQSDVWVEDASIAALIIQ
                     LQAESLGLGSCWVQIRNRRDPEDKDSEKFVRELLGIPEQYGVLCILALGMKAEQKKPF
                     DESKLQWEKLHLGHYKK"
     gene            1726..>1826
                     /locus_tag="PKY68_09315"
     CDS             1726..>1826
                     /locus_tag="PKY68_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOH25265.1"
                     /translation="MRICLLGAGNVAVHLGKHLQAAGYTLVQVYSRT"
BASE COUNT          465 a          410 c          472 g          479 t
ORIGIN      
        1 gtgctgaggc cgaggttgag gttgcgccgg ggtttgtact ctaagttgag tgcaccaaaa
       61 gccgtacgga acaggtcttt ttccttaccc tcgaaataga cgtaaaattc gtatttattg
      121 ttgaacgttc cgtacttagt tttgcgggtt tcaggtataa aattgtaatt gttttgagag
      181 aaattgccca gaaaggagag ttcccatttg gatgccaggg tataactcat aaaagtttgg
      241 tagtccaaaa acgagggccg gtattctccc ttggtgtcga gggcgccgag caagtaggca
      301 ttggtcttgt agcgcatacc gtgtatttgc ctgaacttgc cgtttttgga agcctgcccc
      361 acaaagacat tggcaccgag cagacttatg gctgccgagc cttcgaattc ttccggtttt
      421 ttgtagcgga tatccagcac cgaggccatt ttgtctccgt acatggcttc gaagccgccg
      481 gccgaaaact tcacctcgtc ggtcatactg ggatttacaa aactcagtcc ttcctgctgg
      541 ccgctgcgaa tcaacagggg acggtaaact tccatgccat tgacatagac caggttttcg
      601 tcgtaatttc ctcccctgac cgaatactgg gaactcaatt cgttgttgga actcactccg
      661 gcaaaagtaa ccagcaaagc ttctatgctg ccgcccgtag ggtcgggcag aaggcggata
      721 ccggaaatgt cgactttttc cagactgcct gttcgtcttt gttgtgcggt cactacggct
      781 tcgtccaggg ttttggaact cgctgtcaag atgacattca gcaccagttg attgacttcg
      841 accgggacga tgcgaacggc ggtctgatac gacaaacaac tgtaatagag gatcagggtg
      901 tcctgccagg gcagctccag ttcataataa ccgctttgat cgctggcggt acctttttga
      961 ctgtgttgct caaaaatatt gaccagttca attgcccgtt tgttggcatc catcacaaaa
     1021 cccgttaact tgaccgtacg atcacgtgca acgagcgaaa gccatcccgg catcaacaaa
     1081 acaaggaaga aaagcaggca tctgaaccgc atcaaaggtc gaattttagg acaaatgtac
     1141 tcgaaatcta cggaattaaa cgtacttttg tcttcgttta ttgtaattct atggataact
     1201 ttatcgactt agtgcgtcaa cgcagaagta cccgcaaatt tatggaaacc tctatcagtc
     1261 ccgacctggt ggccgagctg atgcgggccg ctttgatgtc gccttcttcc aaaaggagca
     1321 atccctggga atttgtattg gtagacgatg cggagatgct caaaaaatta tcattgagta
     1381 aaaagcacgg cagcgcactt atcgaaggag cttcgctggg catagtggtt atcgccgaca
     1441 aaacacaatc ggatgtatgg gtggaggatg cttcgatcgc agccttgatt attcagttac
     1501 aagccgagag cctgggcctg ggttcctgct gggtgcaaat cagaaatcgc cgggatccgg
     1561 aggacaaaga cagcgagaaa ttcgtacgtg aattgttggg aatacccgaa caatatggcg
     1621 tgttgtgtat tttggctttg ggcatgaaag ccgagcaaaa aaagcctttt gacgagtcta
     1681 agctgcagtg ggaaaaattg catctgggac attataaaaa atagtatgag gatttgtttg
     1741 ctgggtgccg gtaatgtggc cgttcatttg ggtaaacact tgcaggcagc aggttacact
     1801 cttgtgcagg tgtatagccg tacgga
//
LOCUS       DAOSGL010000374         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_65186_length_1825_cov_3.297175, whole genome shotgun sequence.
ACCESSION   DAOSGL010000374 DAOSGL010000000
VERSION     DAOSGL010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65186_length_1825_cov_3.297175"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1825)
                     /gene="sprA"
                     /locus_tag="PKY68_09320"
     CDS             complement(<1..>1825)
                     /gene="sprA"
                     /locus_tag="PKY68_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOH25266.1"
                     /translation="GWAAMTNATLAVSDLGTVSFAGRYESVGFGGIEQRVAERRLEDY
                     KLMNLAMSFDLGKLYPKPGIVNFPVYYAISRQSHTPKYDPLNQDLLFEDVLKAANSKA
                     EKDSILDYSSKIMNVRNLNVSNLRLNIKSKTPLPIDPANFSASGSFSETYKHDASTEY
                     EITRNYNAGITYGYSSPLKAWEPFAKSKKLDSPWLKTIKEFNLTWIPNSVTFGSRLSR
                     YYYELQNRDLTSLSLLNNIPLNFSKNFLWNTDFSLTWNFTKNMKFTFATNNSSRIQET
                     VNSPVNKTLYPNEYENWKDTVRQSIRTLGTPLNYQQSIRYTWSIPFNRIPVLDFITAN
                     AQYTSTYKWDKSAEIKDLEMGNIISNIRNININPSFNFETLYNKSEFLRQVNQKYAAS
                     GRKTSTASGRSTSAKVSSNQQSAPKQEAAKFEKELQLSAGEELEVKHNLKNKRLIIRA
                     VDSKGKEVPLRYKIVDENNLSIRGRDSMQIKLLVVQKPPLDDKDWYKALQLGSRLLMS
                     VRNLHITYDHTDGLTIPGFKPETGLIFKDEEFGRAPGWDFASGFYSNDYLQQSMARNW
                     LVMSDSVVSPAIYTESRKLTVRSSVSPFQGLRIELNAQRSNN"
BASE COUNT          454 a          399 c          439 g          533 t
ORIGIN      
        1 tattgttgct gcgctgtgca ttcaattcaa tcctcagccc ctggaaaggt gacacagagg
       61 atctgacggt tagtttcctg ctttcggtat aaatagccgg cgaaaccacc gagtctgaca
      121 taaccagcca atttctggcc atagattgct gcaagtaatc gttggaataa aaaccgctgg
      181 caaaatccca gcccggagcc ctgccgaatt cttcatcttt gaatatcaaa ccagtttccg
      241 gcttgaatcc gggaatagta agtccgtccg tatgatcgta agtgatgtgg agattgcgaa
      301 ccgacataag caaacgcgat cccaattgca aagccttata ccaatcctta tcgtccagcg
      361 gaggcttctg aactacgagc aacttgattt gcatactatc tctgccacga atgctcaggt
      421 tgttttcatc gactatttta taacgcaggg gtacttcctt tcctttggag tctacagccc
      481 ttattattaa acgtttattc ttcagattat gttttacttc gagttcttcg cccgctgaga
      541 gctgcagctc tttttcgaat ttagcggctt cttgtttagg agcagattgc tgattgctcg
      601 atactttggc ggacgtactt ctgccggatg cagtgcttgt ctttctgccg gatgcggcat
      661 atttttgatt gacctggcgt aaaaattccg acttattgta taaggtttca aaattaaaag
      721 aaggattgat gttgatgttt cgaatgttcg aaatgatgtt tcccatctcc agatccttga
      781 tctcggcact tttgtcccat ttataagtgg aggtatattg agcgttggca gtaataaaat
      841 ccagtacagg aatgcggttg aaaggaatgc tccaggtata cctgatactt tgttggtagt
      901 tgagcggcgt gccgagggtg cggatgctct ggcgcaccgt gtctttccag ttctcgtatt
      961 cgttgggata gagcgttttg ttaaccggag aattcacagt ttcctggatg cgggaactgt
     1021 tgttggtagc aaacgtaaat ttcatattct tggtaaaatt ccaggtcagg ctgaaatcgg
     1081 tgttccagag aaagtttttg ctgaaattca agggaatgtt gttgagcagg ctcaaagaag
     1141 ttaaatcgcg attctgaagc tcataataat agcgcgacaa acgactgccg aaagtgacgg
     1201 aattgggtat ccaggtcagg ttgaattcct taatcgtttt taaccaggga gaatccaatt
     1261 tcttgctttt ggcaaagggc tcccaggcct tgagcggaga actgtagccg taagtgatgc
     1321 cggcgttgta attacgggtg atttcgtatt ccgtactggc atcgtgttta taagtttcgc
     1381 tgaaactgcc ggaggccgaa aaattggccg gatctatggg cagaggcgtt ttgctcttaa
     1441 tattcaggcg taggttcgat acatttagat tgcggacatt cataattttg gaactgtaat
     1501 ccagtatgga atccttctcc gccttggaat tggctgcctt cagcacatct tcgaacagaa
     1561 gatcctgatt caaaggatcg tatttaggcg tatggctctg acgagaaatg gcgtagtaga
     1621 ccggaaaatt cacaattccc ggtttgggat aaagcttgcc cagatcgaaa ctcatcgcca
     1681 ggttcatcaa tttataatct tccagcctgc gttcggccac tcgctgttca ataccgccaa
     1741 aacccaccga ctcgtaacgg cctgcaaaag agaccgttcc cagatcggac acagccaggg
     1801 tggcgttggt cattgccgcc caacc
//
LOCUS       DAOSGL010000375         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_65467_length_1820_cov_3.176771, whole genome shotgun sequence.
ACCESSION   DAOSGL010000375 DAOSGL010000000
VERSION     DAOSGL010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65467_length_1820_cov_3.176771"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..875)
                     /gene="rfbA"
                     /locus_tag="PKY68_09325"
     CDS             complement(15..875)
                     /gene="rfbA"
                     /locus_tag="PKY68_09325"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOH25267.1"
                     /translation="MKGIILAGGSATRLFPLSIAISKQIMPIYDKPMIYYPLSTLMLA
                     GIREVLVISTPRDLPMFRELLGSGESLGMSFQYKVQEQPNGLAQAFVLGADFLNGGPA
                     CLILGDNLFYGQGFSSMLKRAVALERGACIFGYYVKDPRPYGVVEFDENGKVLSLEEK
                     PSQPKSNYAVPGLYFYDERVTRFASELKPSARGEYEITDLNKVYLKEGSLQVELFGRG
                     FAWLDTGNSDSLLEASNFVATIQNRQGFYVSCIEEIAWRNRWIDNEQLRKLGEKLNKT
                     AYGQYILSLV"
     gene            1122..1586
                     /gene="mscL"
                     /locus_tag="PKY68_09330"
     CDS             1122..1586
                     /gene="mscL"
                     /locus_tag="PKY68_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOH25268.1"
                     /translation="MSFIGEFKKFALKGNVVDMAVGIIIGAAFSKIVSSAVADVIMPP
                     IGLLVGGVNFTDLVWKIKPETLDEMGNVIAPAVTLNYGNFLQVTLDFLIVAFAVFLLV
                     KFINELTKKTEELTRKKQAEETEAPAEPVQPVMSKEESLLTEIRDLLAEKIK"
     gene            complement(1631..>1820)
                     /locus_tag="PKY68_09335"
     CDS             complement(1631..>1820)
                     /locus_tag="PKY68_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HOH25269.1"
                     /translation="FTGILPPSDLLMLMLTQTGMKTAYEILVLPLTSKIVVYLKKVEK
                     VEVFDRDISYNPFKISDL"
BASE COUNT          529 a          398 c          367 g          526 t
ORIGIN      
        1 gacaaaataa gttttcatac caaagataaa atgtattgcc cgtaggctgt tttatttaat
       61 ttttctccca gttttcgcaa ttgttcatta tcgatccaac ggttacgcca tgctatttct
      121 tcgatgcaag acacgtaaaa accctggcga ttttggatgg tagccacgaa attggaggct
      181 tccagcaaac tgtcgctgtt acctgtatcc agccaggcga agccgcggcc aaacaattcc
      241 acctgcaaac tgccctcctt caggtatact ttgttcaggt ccgtaatttc atattctcct
      301 ctggccgagg gtttaagctc ggaagcaaaa cgagtgaccc gctcatcgta aaaatataga
      361 cctggaacgg cgtaattcga tttgggctgc gagggttttt cttcgagaga caaaaccttg
      421 ccgttttcat cgaactcaac cacaccgtaa ggacggggat ctttcacgta atatccgaag
      481 atacaggctc cccgttcaag agcaacagca cgttttaaca tcgaggaaaa gccctggccg
      541 taaaacaggt tatcacctaa aatcagacaa gccggccctc cgtttaagaa atccgctccc
      601 aacacaaaag cctgagccag accattgggc tgctcctgta ctttatattg aaacgacatc
      661 cccagcgatt ctccgctgcc gagtaattcc cggaacatag gtaaatctct gggagttgaa
      721 atcaccaaca cttcgcgtat gccggccaac ataagtgtag acaaggggta ataaatcatg
      781 ggcttgtcgt aaataggcat gatttgtttg gaaatagcga ttgacaaagg gaacaatcgg
      841 gtggcgcttc cgccggctaa tataattcct ttcattgtgg gttttttagg ttttgcgaga
      901 aaacacaaag ataagcatac acttaaatat tgcaaactta tgcccctgct ttatttcctt
      961 ttttgaagga atgtttcgtg catatttatt ctaaattatt gtttacattt gccccggtgc
     1021 aaggagtaca ttttacttcc atctccggca ttttaatcct tgtttaaact ttttgatttt
     1081 attctttgtt attcaattat tcacccttta tttaaatcct tatgagtttt atcggagaat
     1141 ttaaaaagtt tgccctcaaa ggcaacgtag tagacatggc tgtcggtatc attatcggag
     1201 ctgcctttag taagattgta tcctctgcgg tggctgatgt catcatgccc cctattgggt
     1261 tgttggtagg cggtgtcaat tttaccgacc tcgtctggaa aatcaaaccc gaaacgctgg
     1321 acgaaatggg caatgtaatt gcacctgccg taacgttgaa ttacggtaat tttctgcaag
     1381 ttaccctcga ttttctgatt gttgcttttg ccgttttcct tttggttaaa tttataaacg
     1441 aactgaccaa aaaaacggaa gaactcacca ggaaaaaaca agctgaagaa acggaagctc
     1501 ccgcagaacc cgtccaaccg gttatgtcca aagaagagag tttattgacc gaaatccgcg
     1561 atttattggc tgaaaaaatc aagtaaaaga acagcctgaa taaggtcgaa attcaaagaa
     1621 ggcaaaatct ctataaatca ctaattttaa atggattata agagatatcc cggtcaaaga
     1681 cttcgacttt ttctactttt ttcagataaa caacaatttt tgaagttaaa ggcagtacaa
     1741 gaatctcata ggccgtcttc attccagttt gggttagcat caacatcaac aggtccgagg
     1801 gcggcaatat gcctgtaaaa
//
LOCUS       DAOSGL010000376         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_67076_length_1790_cov_3.700288, whole genome shotgun sequence.
ACCESSION   DAOSGL010000376 DAOSGL010000000
VERSION     DAOSGL010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67076_length_1790_cov_3.700288"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKY68_09340"
     CDS             <1..228
                     /locus_tag="PKY68_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH25270.1"
                     /translation="TVIKRGTSISINCDDEVFNSPAQLEITSLGGQKLYQSRLLEKNS
                     SISLPAEVSPGIYMLVIQHPGGKTVQKIVVE"
     gene            384..>1790
                     /locus_tag="PKY68_09345"
     CDS             384..>1790
                     /locus_tag="PKY68_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOH25271.1"
                     /translation="MRRRTILLGLFIYFGLSMATAQSGSEIVSGKVRDELGPLVMVNV
                     IELDEANRVVSHDVTDINGNFSIRIKSPRNKLRFSYVGYETLTVPIDRKVFDIVMADA
                     MVLQAAEITGRKAITQTGGLSIPERELSMALQSIDMSEFEGLSFTSIDEALQGRIAGL
                     DIVVNSGNLGSGSSMRLRGVSSINANSEPLIVVDDNVQAVDQTDFDVATANEERFAQL
                     LNINVEDIESVTVLKDAAATAIWGSQGANGVILIKTRRGAKGPARLTYSYKFSGTHQP
                     KGLNMLTGDQYTMMLKEAYFNPTLSDAAANIIELNYDPTFSEYQQYNNNTDWREQVIK
                     FGQKHSHYLSLSGGGDKANFRVSGGYDGETGTIIAQKLDRFSTRVALDYFVSERIKIM
                     SNFALTYTNNQRNSDALLNLAYRKMPNLTVFEQDIMGNDTHEYYQVLQSCSNELSDQR
                     NLVNPVASAYLAKNLDRNY"
BASE COUNT          494 a          398 c          437 g          461 t
ORIGIN      
        1 actgtaatca agagaggaac aagcatcagc ataaactgcg atgatgaggt atttaattct
       61 ccggctcaac tcgaaatcac gagcctggga ggacagaaat tgtatcaaag ccgtctgctt
      121 gaaaagaatt cttccataag cttacctgct gaagtttcgc ccggaattta tatgctcgtc
      181 attcagcatc cgggaggaaa gacagtacag aagatcgtgg tagaatagaa aaacagactt
      241 tgcatacatt cttgtatttg tcgctttgga gacaataatg tttgataaat ggattttttt
      301 atcatttcaa gcagtgaatg aactatattt ttgtgtgagt tactctaaat gtaataatca
      361 atgaaaacac aactgttatg cttatgcgca gaagaacaat tttactaggc ctttttattt
      421 atttcggcct cagtatggct acggcccaat caggttctga aattgtcagt ggcaaggtgc
      481 gcgatgagct gggacctttg gtgatggtga acgtcattga gctcgacgag gctaaccgtg
      541 tagtatcgca cgacgtaacg gatatcaacg ggaatttttc cattcgtatc aaaagccccc
      601 gaaacaagct caggttttct tacgtgggtt acgaaacgct gacggtaccc atagatcgta
      661 aggtttttga catagtaatg gccgatgcca tggtgcttca ggctgccgaa ataacaggca
      721 gaaaggccat tacacagacg ggaggtctca gtattcccga gagggaattg tctatggctt
      781 tgcagagcat agacatgtcg gaattcgagg gactgagttt tacctctatc gatgaggcct
      841 tgcagggacg catagcgggt ctggatattg tggttaattc cggtaatctg ggcagcggtt
      901 cttccatgcg cctgaggggg gtgtcttcca tcaacgccaa ttcggaacct ttgattgtag
      961 tggacgacaa tgtgcaggcc gtcgaccaaa ccgattttga tgtggctacg gccaacgagg
     1021 aacgctttgc tcaattgctg aacatcaacg tggaagacat cgagagtgta acggttttga
     1081 aagacgctgc cgctaccgcc atttggggtt ctcagggagc caacggggtt attttgatta
     1141 agactcgcag gggcgccaaa ggccctgccc ggcttaccta ttcctacaag ttttcgggaa
     1201 cgcatcaacc caaagggctc aatatgctga caggagatca atacaccatg atgctcaaag
     1261 aagcctattt caacccgact ttgagcgatg cggccgccaa tattattgag ctgaattacg
     1321 atcccacttt ttccgaatat cagcagtaca acaacaatac cgattggcgg gagcaggtca
     1381 taaaattcgg gcaaaagcac agtcactatc tttcgttgtc gggcggaggc gataaagcta
     1441 atttccgtgt ttcgggtggc tacgatggtg aaaccggtac gatcattgcc caaaagctgg
     1501 atcgtttttc gacccgggtg gcgctcgatt attttgtatc ggagcgcatc aagattatgt
     1561 ccaactttgc cctcacttac accaataatc agaggaacag cgatgctttg ctcaatctgg
     1621 cttaccggaa aatgcccaac ctgactgttt tcgaacagga tataatgggc aacgacaccc
     1681 atgaatatta ccaggtactc caaagctgtt ccaatgaact cagcgaccag cgcaacctgg
     1741 tcaatccggt agccagcgct tatctggcca aaaacctcga cagaaattac
//
LOCUS       DAOSGL010000377         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_67593_length_1781_cov_2.206837, whole genome shotgun sequence.
ACCESSION   DAOSGL010000377 DAOSGL010000000
VERSION     DAOSGL010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67593_length_1781_cov_2.206837"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PKY68_09350"
     CDS             complement(<1..866)
                     /locus_tag="PKY68_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOH25272.1"
                     /translation="MSPMEEQNVNPQATQQAEDEIDLIAIAKTLWKGRKIVLIATGIG
                     AVLGLLVALLTPKEFTVKTVLVPQMSGGKSSQLSGLASLAGVDLGMAESAELSPIIYP
                     QIVQSVPYMLELMHTPLHFSKVDTPVSYFEYSTEVKKPSVLGVVKKYTIGLPSVLLSA
                     LRKAPEELVLPGDMSDKPIAMSRKEYDLKKSLSEKISLTVDKKEGYLRLTVGMPEALA
                     AAEMAQKAQNLLQQYIIAYKVEKSQAELDFIQERYDAAKEEAEKQQVEFATLSDQYKD
                     LTSRVPQVELQKV"
     gene            complement(866..>1781)
                     /locus_tag="PKY68_09355"
     CDS             complement(866..>1781)
                     /locus_tag="PKY68_09355"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOH25273.1"
                     /translation="SFTFDPSMSLQDLLINAGGFNVGAAFNRVEVFRLQLSPSEKVKL
                     ELITLEVDSSYQVVKPYNFSLQPYDLVVVRMTPEFTTGRVIELNGQVKYPGPYVLEDK
                     KTTLRDVIKQAGGLLDDANPYGAQLIRTYNNRGFISINLKKAMNSGSRINHNPILFDG
                     DVINIRRTENVVAILENGTRMAQYAPDPDNYQIKNVVYQGPKSAKWYVRKFAGGFDKN
                     ANRRSVTVTHANNQMVGTDCFLFVRDYPTVQPGSTITLQLDPEKIRKESEPKEKVDLE
                     STVARSLATLTSTLSLILLVQQLAKAAP"
BASE COUNT          336 a          449 c          508 g          488 t
ORIGIN      
        1 actttctgca gttctacctg aggcacccgg ctggtcaggt ctttgtattg atcggagagt
       61 gtggcaaatt ccacttgctg cttctctgcc tcctctttgg cggcgtcgta gcgttcctgg
      121 ataaaatcga gctctgcctg cgatttctcc accttatagg caataatata ctgctgcaat
      181 aaattttggg ccttttgcgc catttcggcg gctgccaatg cttcgggcat accgacagtc
      241 aacctcaggt agccttcttt cttatccacc gtcaggctga ttttttcact caggcttttc
      301 tttagatcgt attcttttct gctcatagcg atcggcttat cgctcatatc tccgggtaag
      361 accaattctt cgggcgcttt gcgcaaagca gacagcaaca cactgggcaa acctatggtg
      421 tacttcttta ccacacccaa caccgaaggt tttttcactt cggtagaata ctcaaagtac
      481 gacaccggcg tatctacttt actgaaatgc agaggggtat gcatcaattc aagcatataa
      541 ggcaccgatt gcacaatctg gggataaata atgggcgaaa gttctgcgct ttcggccata
      601 cccaggtcca ccccggccaa cgaggccagg ccgctcaatt gcgagctttt tcctccgctc
      661 atttggggca ccaacactgt tttgacggtg aattctttgg gcgtcagcaa ggccaccaac
      721 aggcccaata cggcgcctat gccggtggcg atcaaaacga ttttgcggcc tttccagagg
      781 gttttggcaa tcgcaatcag gtctatttcg tcctcagcct gctgcgttgc ctgagggttt
      841 acattctgtt cttccatagg gctcattagg gggcggcttt agcgagttgt tgtactaaga
      901 ggatcagcga gagggtggag gtcagggtgg ccagactgcg ggcgacggtg ctctccagat
      961 cgactttttc tttgggttcg ctttctttgc ggatcttttc gggatccagt tgcagagtga
     1021 tggtggagcc gggctgcacg gtggggtagt cgcgtacaaa gaggaagcag tcggtgccga
     1081 ccatctggtt gttggcgtgg gttacggtta cgctgcggcg gttggcgttt ttgtcgaagc
     1141 cgccggcaaa tttgcgcaca taccatttgg ccgatttggg gccctgataa accacgtttt
     1201 tgatctggta attgtccggg tcgggggcgt actgggccat gcgggtgccg ttttccagga
     1261 tggctaccac gttttcggtg cggcggatgt tgatcacgtc tccgtcaaag aggatggggt
     1321 tgtggttgat ccggctgccg ctgttcatgg cctttttcag gttgatactg ataaagccgc
     1381 ggttgttgta ggttcggatc agttgggcgc cgtaggggtt ggcgtcgtcg agcaggccgc
     1441 cggcttgttt gatcacatcg cgcagcgtgg ttttcttgtc ctccagcaca taggggccgg
     1501 ggtattttac ctggccgttg agttctatca cccggccggt ggtaaattcg ggggtcatgc
     1561 gcaccaccac caggtcgtag ggttgcaggc tgaaattata cggttttact acctgataac
     1621 tgctgtccac ttcgagggtg atcagctcca gcttaacttt ttcgctgggc gagagctgca
     1681 ggcggaagac ttccacccgg ttgaaagccg cgcctacatt aaagcccccg gcattgatca
     1741 gcaggtcctg caggctcatg ctggggtcga aggtgaagct g
//
LOCUS       DAOSGL010000378         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_69926_length_1741_cov_4.371293, whole genome shotgun sequence.
ACCESSION   DAOSGL010000378 DAOSGL010000000
VERSION     DAOSGL010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69926_length_1741_cov_4.371293"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKY68_09360"
     CDS             <1..418
                     /locus_tag="PKY68_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH25274.1"
                     /translation="AQRDVGIIVRVPLASGLLSGKFTINTSFGKDDHRTFNRQGEAFD
                     KGETFSGVDYAQGLAAVEEIKKLFPAGQPLSTFALRWILMFSQVSTVIPGASKPEQVQ
                     SNVQATKLPALTEEQMQGIRKIYRDRIWPLIADEVW"
     gene            complement(576..>1741)
                     /locus_tag="PKY68_09365"
     CDS             complement(576..>1741)
                     /locus_tag="PKY68_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HOH25275.1"
                     /translation="IKEHVYGRLIEHGYNHLQVNWLLSLCCFGQYYKDGPEPECSDLT
                     LYEEGKASSTMNLDVWKRMEQHLGFLNNKDIGVHMFLGVDGSRNDGPAWTALSAGEKD
                     FYVRYMVARLAPYANLAGWNYVWEVPGNREEQELGFARLVSQYDIFNHLRTYEDEFPR
                     EHYYHLPEYSFAAIENHQIAAPGKNLERHLWRDAWTHHMACLLGYAGKPVFMSEGNAL
                     WRRYWHERTGATQDDLRRSAWACATAGASFTWNGHASEYELFAYGPEGLPFSEDNEFK
                     LSENYIDILTHVMRNEVDFYRMKPHDELLAHQQALRVYALAEPGQQYLVFAPDGESFS
                     LQLEAGDYSRCCWIDSKSGKKQALPPTQSAGPEQAKAFTPPNKDTDWVLVVRK"
BASE COUNT          384 a          442 c          471 g          444 t
ORIGIN      
        1 agcccaaaga gacgtcggca ttattgtgcg ggtgcctctg gccagcggct tactgagcgg
       61 taaattcaca ataaacacca gctttggcaa agacgaccat cgcacattta atcgtcaggg
      121 cgaagctttc gataaaggag aaactttttc cggtgtagat tacgctcaag gcttggccgc
      181 tgtagaggaa atcaaaaaat tgttccccgc cggacagcct ttatcgacgt ttgcgctccg
      241 atggattctg atgttcagcc aggtaagcac ggtaatcccc ggggcttcca agcctgaaca
      301 ggtgcaatcc aacgtacagg ctacgaaatt acctgctctg acggaggaac aaatgcaggg
      361 gattcgcaag atctaccggg acagaatctg gcctttgatt gccgacgaag tctggtaaaa
      421 gcagcatccg tccgtttaaa cggcagtatc ttgcccgtct gattatcgat tcggacgttc
      481 ggtttctgtt tccgaaccgg gttttatttt cgaacttcga gctgtttctt aaccttgagc
      541 tgtttcccaa tctcgagctg ttttccaacc tcgaattatt tcctaaccac gagcacccag
      601 tcggtgtctt tgttgggcgg cgtaaaagcc ttggcttgtt cggggccggc gctttgcgtt
      661 ggcggcaatg cctgcttttt gcccgacttg ctgtctatcc agcaacagcg gctgtagtct
      721 ccggcttcga gctgcagcga aaagctttct ccgtcgggag cgaaaaccag gtattgttgt
      781 cccggttcgg ccagggcata cactcgcaga gcttgctgat gggccaggag ttcgtcgtgg
      841 ggtttcatgc gatagaaatc cacttcgttg cgcatcacat gggtcagaat gtctatgtaa
      901 ttttcggaga gtttgaattc gttgtcttcg ctgaaaggca ggccttcggg tccgtaagca
      961 aagagttcgt attcgctggc gtgtccgttc caggtgaaag aagctcctgc ggtggcacaa
     1021 gcccaggccg agcgacgcag gtcgtcctgg gtggcgccgg tgcgttcatg ccagtagcgt
     1081 ctccataggg cgttgccttc gctcataaac accggtttcc cggcgtaacc caacaggcag
     1141 gccatgtggt gggtccaggc atcccgccag agatgacgtt ccagattttt tccgggagcg
     1201 gcaatctggt gattctctat ggcagcaaag ctgtattcgg gcaaatgata atagtgttcg
     1261 cgcgggaatt cgtcctcgta agtacgtaag tggttaaata tatcgtattg gctgaccaga
     1321 cgggcaaagc ccaattcctg ttcttcccgg ttgccgggta cttcccagac gtaattccag
     1381 ccggccagat tggcgtaggg agccaagcgg gctaccatgt agcggacgta aaaatctttt
     1441 tctccggcac tgagagcagt ccaggccgga ccgtcgttgc ggctgccgtc cacacccaaa
     1501 aacatatgca caccgatgtc tttgttgttg agaaagccca ggtgttgctc catgcgtttc
     1561 cagacgtcaa ggttcatggt agaagaggct ttgccctctt cgtataaagt cagatcggaa
     1621 cattcgggtt cgggtccgtc tttgtaatat tgaccaaaac agcagagtga caaaagccag
     1681 ttcacctgta aatgattgta accgtgttct atcaggcggc cgtaaacatg ttcctttatc
     1741 c
//
LOCUS       DAOSGL010000379         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_70574_length_1730_cov_4.488955, whole genome shotgun sequence.
ACCESSION   DAOSGL010000379 DAOSGL010000000
VERSION     DAOSGL010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70574_length_1730_cov_4.488955"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKY68_09370"
     CDS             complement(<1..533)
                     /locus_tag="PKY68_09370"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOH25276.1"
                     /translation="MLLPFLQEGRIEAGCDEAGRGCLAGPVFAAAVVTAADFDCPEIN
                     DSKQLSYKERYRLRAEIEKRALAWGIGTASPEEIDKINILNAALLAMHRAVLHLQLSP
                     DYLLIDGNRYRKPSQLPIHKISVSVDEFSKIEHIPAGCFVKGDGRFVSIAAASILAKT
                     YRDDYMQNLHKQYPQYA"
     gene            complement(537..1571)
                     /locus_tag="PKY68_09375"
     CDS             complement(537..1571)
                     /locus_tag="PKY68_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOH25277.1"
                     /translation="MLLAPKTPQRLSLKEISVIPSLATALFLLLLMTSCMSGQKKNSP
                     SGQNLKVVPVFNEDSAYHYLSRQLDFGPRVPNTEGHKACGDYLVEKMRSLGGILTEQP
                     MQLEAFDGNVLRARNIIASFDPEKTRRILLFAHWDTRPWADEDPDPQNHTKAIPGAND
                     GASGVAVLMEIARHLGTTTPSSLGVDIILFDAEDYGQPSFSGRSRVEDSWCLGSQYWA
                     KNPHVSNYSAEFGILLDMVGAKGAIFAKEYFSLRYAAPVVNKVWNTAARIGYSHYFEN
                     SEGGAITDDHLYINRLAGIPSINIIDYRSDTPNGFFPYWHTLEDSLDKIDKKTLKAAG
                     QTLLELIYTE"
BASE COUNT          421 a          386 c          450 g          473 t
ORIGIN      
        1 caggcatatt gagggtattg cttatgtaaa ttctgcatat aatcgtctct gtatgttttg
       61 gccagtatgg aggctgcagc aatggaaaca aatcttccgt ctccttttac aaaacaaccg
      121 gccgggatat gttcaatttt actgaattca tcgacactta cggatatctt atgtatgggt
      181 aattgtgaag gcttgcggta gcgatttcca tcgatgagta agtagtcggg actaagctgt
      241 agatgcaaca cagccctgtg catagccagg agagctgcgt tgaggatatt gattttatcg
      301 atttcttcgg ggctggcagt gccgataccc caggccagtg ctcgcttttc gatttcggct
      361 ctcaggcgat agcgttcttt gtagctgagt tgtttggaat cgttgatttc cggacagtca
      421 aagtcggctg ccgttacgac ggctgccgca aagaccggcc cggccagaca accccggccg
      481 gcttcgtcac aaccggcttc aatacgtccc tcctgtaaaa aaggcaagag catggtttat
      541 tcggtataaa tcaattccaa cagggtttgg cctgctgctt tgagggtttt tttgtcaatt
      601 ttgtccagac tgtcttccag ggtatgccaa taagggaaaa atccatttgg agtgtcgctg
      661 cggtagtcga tgatgttgat gctcgggatg cctgccaggc ggttaatgta cagatgatcg
      721 tcggtaatgg cgccaccctc cgagttttca aaataatgac tgtaacctat tcttgcagct
      781 gtgttccaaa ctttgttgac aacaggggct gcatagcgta gggaaaagta ttctttggca
      841 aaaatagccc cttttgcacc aaccatatcc aacaaaatgc cgaattcagc agaataattg
      901 ctgacgtgag gattttttgc ccagtactga gaaccgaggc accaggaatc ttccacccta
      961 gatctgccgg aaaaagaggg ttggccataa tcttcggcat cgaacaaaat aatgtctacc
     1021 cccaaagaag aaggggttgt cgtacccaga tgccgggcaa tctccatcaa gacagccact
     1081 ccgctggccc cgtcgttggc tccgggaatg gcctttgtat ggttttgcgg gtcgggatct
     1141 tcgtcagccc aggggcgggt gtcccagtgg gcaaagagta aaattcttcg cgttttttcc
     1201 ggatcaaacg atgcaataat gtttctggct cttaggacgt tgccgtcgaa ggcctccagt
     1261 tgcataggct gttcggtaag gattccgcca agcgagcgca ttttttctac caggtaatcg
     1321 ccgcaggcct tatgtccttc cgtattggga acccggggac cgaaatcaag ttgccttgac
     1381 aggtaatgat aggctgagtc ttcattaaaa acaggcacca ccttaaggtt ttgcccggag
     1441 ggagagttct ttttttgtcc cgacatgcaa gaggtcatca aaagaagtaa aaacaaagca
     1501 gtagccaaag aaggtatgac gctgatttct ttaagcgata atcgttgggg tgttttagga
     1561 gcaaggagca ttatggtgca tttttagggt tttcagcata aagttgagcg agttccaggg
     1621 ttctgataaa attgcctccg gttattaaag cgatttcttc gtcacgatag ccttcggctt
     1681 tgagggctct cagcagatgg atgagttcac cggcgttttg gcaaccgggt
//
LOCUS       DAOSGL010000380         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_70918_length_1725_cov_3.810180, whole genome shotgun sequence.
ACCESSION   DAOSGL010000380 DAOSGL010000000
VERSION     DAOSGL010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70918_length_1725_cov_3.810180"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..893)
                     /locus_tag="PKY68_09380"
     CDS             complement(192..893)
                     /locus_tag="PKY68_09380"
                     /inference="COORDINATES: protein motif:HMM:NF036640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like C-terminal domain-containing protein"
                     /protein_id="HOH25278.1"
                     /translation="MKILRNKLLPLFVVYGLISCNNEYGLMMEPAAEIWQSGLCVAVP
                     HEDYPYVLLMDDGDRIVPSGEPESNVKIGNRYSVYYSLIKPKQEDEVRKAEIAKMIPV
                     TTIPLTYPDRIRLNSVQGDPIEITKAFWLGGDFINVEFNFKISDRQIKHSLELHYISF
                     DEEYKELKLDFIHMANGDLPEQDLPAIVSFPLESIYEFNKADTILIRVMQKNRQDENH
                     YSFYGLKGGYVEDLR"
     gene            complement(874..1581)
                     /locus_tag="PKY68_09385"
     CDS             complement(874..1581)
                     /locus_tag="PKY68_09385"
                     /inference="COORDINATES: protein motif:HMM:NF036640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like C-terminal domain-containing protein"
                     /protein_id="HOH25279.1"
                     /translation="MKNIYKAISVFLLILPLASCSGFWGENKPDSVYYESMANVQSIK
                     TNNVIFKSNPSLYFTSQDGFDLDDSIAEGDRVWLYFTLVDSVSPSMYNIHIYSYSEVD
                     TRPFYQLEKGANDTILSQNLHQFQKCWISDNYLNVIFYAYASISPNSSFELVREMIRE
                     DTSASKPKIYLDLRHNTASVTPYWYNLKMMSFDLRPIREQYAGQDTISVQFSYTLSDR
                     QHVNFYYLPQADENSQE"
     gene            complement(1587..>1725)
                     /locus_tag="PKY68_09390"
     CDS             complement(1587..>1725)
                     /locus_tag="PKY68_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOH25280.1"
                     /translation="REQFSDYLQQQLNKIELPDKDEPFLRKKMLEMYANPLKNKQKAK
                     A"
BASE COUNT          480 a          343 c          385 g          517 t
ORIGIN      
        1 agaacacaag agcgctagca caagcagact gcttgtgaaa atacgatgaa tgattgtcat
       61 ggttgtaacg atttagataa aaattttgcg ccaaagtact atataattcg tcagcctcgc
      121 aaaattttaa atttttatca acaaaactat gttaataagg tgctgatttt tttgtcccgg
      181 tcttgggcaa actatctcag gtcttcgaca taaccaccct tcaggccgta aaaactgtag
      241 tggttttcgt cctggcggtt tttctgcata acgcgaatca gaatggtgtc ggccttattg
      301 aattcataaa tggattcgag cgggaacgat acaatggcgg gcaaatcctg ttccggcagg
      361 tccccgttgg ccatatggat aaaatcgagt ttcaactctt tatactcttc atcgaaagat
      421 atatagtgga gttcaagact gtgtttgatc tgcctgtctg agattttaaa gttgaactcc
      481 acgtttataa agtcaccgcc caaccaaaac gctttggtta tttctatggg atcgccctgg
      541 acggagttta gtctaatacg gtctgggtaa gtcaggggaa tggtagtgac aggaatcatc
      601 ttggcaattt ctgccttgcg tacttcatcc tcttgtttgg gtttaatcag actgtaataa
      661 acgctgtagc ggttgccgat ttttacattg ctttcgggtt ctccgctggg aacaattcta
      721 tccccatcgt ccatcagcaa gacataagga taatcttcgt gcggaactgc cacacaaaga
      781 cccgactgcc aaatttcagc agcaggctcc atcatcaggc cgtattcgtt gttgcaagag
      841 atcaggccat atacgacgaa caagggcagt aacttattcc tgagaatttt catcggcttg
      901 aggcagataa tagaaattga cgtgttgcct gtcgctcaga gtgtagctaa attgtacaga
      961 gatagtatcc tgaccggcat attgttcacg tatgggcctt aaatcaaagc tcatcatttt
     1021 taggttgtac cagtaagggg taacagaagc tgtattatgc ctgagatcaa ggtaaatctt
     1081 gggtttagaa gccgaagtgt cttcccgtat catttcgcga accagctcaa aactgctgtt
     1141 gggtgaaatg ctggcatagg cataaaagat aacattcagg taattgtcgc tgatccagca
     1201 tttctgaaat tggtggaggt tttggcttaa tatagtgtcg ttggcaccct tttcgagttg
     1261 ataaaaagga cgtgtatcta cttcggaata ggagtagatg tgaatattgt acatgctggg
     1321 cgaaaccgag tcaaccaggg taaaatacaa ccagactctg tcgccctcgg caatggaatc
     1381 gtccaaatcg aagccgtcct gcgaagtaaa atacagactg gggttggatt tgaagatgac
     1441 gttattggtt ttgatacttt gcacattggc catggactca taataaaccg aatccggctt
     1501 attttctccc caaaaaccgg aacacgaagc cagaggtaaa ataagaagga ataccgaaat
     1561 agctttatag atatttttca taagtctcac gcttttgctt tttgtttgtt ttttaaagga
     1621 ttggcgtaca tttctagcat tttttttctc aaaaatggct catccttgtc gggaagctca
     1681 attttattca attgctgttg caaatagtcc gagaactgtt ctctt
//
LOCUS       DAOSGL010000381         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_72986_length_1692_cov_4.157605, whole genome shotgun sequence.
ACCESSION   DAOSGL010000381 DAOSGL010000000
VERSION     DAOSGL010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72986_length_1692_cov_4.157605"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="PKY68_09395"
     CDS             complement(<1..151)
                     /locus_tag="PKY68_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOH25281.1"
                     /translation="MNKVVSRTELSDLVVRLEVEAPMIAKARKPGHFVILKLGAKGER
                     IPLTIS"
     gene            353..1066
                     /locus_tag="PKY68_09400"
     CDS             353..1066
                     /locus_tag="PKY68_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOH25282.1"
                     /translation="MIAFKALIFDLDGTLIDSLQDIADAMNRVLTARKLPPHSYADYK
                     EFVGRGLKNLVERCLPAKLLDPEILEACYNELIRDYREHYVVKTHLYPGIRELLEQIS
                     LKYAGQQKAQTAGGGRLFPLAVLSNKADEITQLIARQLGLTDYFDLILGATPRFPRKP
                     DIQSSLYIAEQLGCQPAEMLYIGDSGVDMQTAKKAGMTAVGVTWGFRSRQELLDNRAD
                     YVVDTPAEILEILRKSLLP"
     gene            complement(1063..>1692)
                     /locus_tag="PKY68_09405"
     CDS             complement(1063..>1692)
                     /locus_tag="PKY68_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOH25283.1"
                     /translation="ADYFEHLRYRLREKVGLLRERTPDYLLYTLLESILDNYFKTLDK
                     LDEQTQAFDFSNPDSDPAPSVLAQIEQYKRYVQFIKKAIVPIKEFTFSIERNKSPYIQ
                     QRHMKYFLEIRDLCLTLIDQCDSILSSLESSNNLFFSVQGQRMNQVMKTLTIVSTIFI
                     PATFVAGIYGMNFINIPELRWHYGYFAVWFLILLIIVGMLIYFKKKKWF"
BASE COUNT          465 a          416 c          396 g          415 t
ORIGIN      
        1 acgaaatggt caggggaatt cgctcgcctt tggcccccag ttttaaaata acaaagtggc
       61 ccggtttcct ggctttggct atcatgggtg cctccacctc caaccttacc actaagtcgg
      121 acaattcggt cctgctgaca accttattca taagcttagt ttatctgttg agtccggatt
      181 tccgaaagag ctcaacgcgc agaaatcacg aaagcctatt cccgttccga acgaaatcag
      241 cacaaaagta ctaaatttga ttgtaccaca gcgagtagaa agggcaaata tttatatcta
      301 tctttgcaaa ctcagccact gaggcctgaa acaaattcta caatcgttgc ctatgattgc
      361 ttttaaagcc cttatttttg acttggacgg caccttgata gattccctgc aagatattgc
      421 agatgccatg aatcgtgtgt tgacagcccg taagctaccc cctcattctt acgccgatta
      481 caaagaattc gtcggccggg gcctgaaaaa tctggttgaa cgatgtctgc ctgccaagct
      541 gctggatccc gaaatactgg aagcctgtta taacgaactg atacgtgact accgcgaaca
      601 ttatgtagta aagacccatc tctatccggg cattcgtgag ctgctggagc agatttcact
      661 caaatacgcc gggcaacaga aggcccaaac tgccgggggc ggcaggcttt tcccgctggc
      721 cgttctctcg aacaaagccg acgaaattac ccaactcatc gcccggcaac tggggctgac
      781 cgattatttc gacctgatcc tgggtgccac gccccgtttt ccccgcaaac ccgatattca
      841 gtcgagcctg tatatagccg aacagttagg ctgccaaccc gccgaaatgc tctacatcgg
      901 cgacagcggc gtagatatgc aaaccgccaa aaaagcagga atgacggctg tgggcgtcac
      961 atggggcttt cgcagccggc aggaactcct cgacaacaga gcggattatg tagttgatac
     1021 tccggcagaa atcctggaga tactccgaaa gagcttattg ccctaaaacc acttcttctt
     1081 tttgaaataa atcagcatcc ccactattat cagaagaatc agaaaccaga cggcaaaata
     1141 accgtaatgc cagcgcagct cggggatatt gataaagttc atcccgtaaa tgccagcgac
     1201 aaaagtggcc ggaataaaaa tagtggaaac aatggtaagg gttttcatca cctggttcat
     1261 gcgttgcccc tgcaccgaga aaaacaggtt gttactactc tcgagcgaag ataaaatact
     1321 gtcacattga tctatcaaag taaggcagag atcgcgaatt tccagaaaat atttcatgtg
     1381 ccgttgttga atgtaggggc ttttattgcg ttctatgctg aaggtaaatt ccttgatggg
     1441 aacaatggct tttttgataa actgcacgta gcgtttgtac tgttcaattt gcgccagcac
     1501 cgagggagcc ggatccgaat ccgggttgga gaagtcgaaa gcctgagttt gctcgtccag
     1561 tttgtctaag gttttaaaat aattgtccag tatggattcc agcagggtgt agagcaagta
     1621 atcgggcgtg cgctcgcgca acagacccac tttttcacgg aggcggtagc gtagatgttc
     1681 aaaataatcg gc
//
LOCUS       DAOSGL010000382         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_73780_length_1680_cov_4.494154, whole genome shotgun sequence.
ACCESSION   DAOSGL010000382 DAOSGL010000000
VERSION     DAOSGL010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73780_length_1680_cov_4.494154"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>1680)
                     /locus_tag="PKY68_09410"
     CDS             complement(165..>1680)
                     /locus_tag="PKY68_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25284.1"
                     /translation="LQSGNPDTITFSSIGTVGTGFMKAERLIAYRVQPANQTGGSGTT
                     PGQGDGIIDLTTLPGNVQPRSMGFATVDVDGNQAVAVTKDQGSGANAEAYVFAPSATS
                     NPFYLNWQAMDRFSSYDLQIKIATGTLSSGTISNPPDTYANGLVFRGMQAQGQQQQFL
                     GISLVRNTMLSAVTPSSWQPWISGTEYSVGVVVSYNNAYYECIQAITLGNSIKPTNDS
                     YWKLFSEDKPMIVLWVRGSNQGNGDGEWLSYKLLDQTGKDFIVDASGHVKNWSTLMVR
                     LVEAASLKLKTNSAPLINIGDTIISGAGSNDSARVFWKINDVDGKVVLLLNNVNGTFS
                     TSTTILNRTLPFDTDPGWGYRPRDNYIWVFYTDTTDHSSNKTPLETSGADDVRHGQIR
                     GNINWPITDVQSWTDTDDSFSLVQWSSSLNQTVDPTIRVMGIGKEAGAIIRTAIVNPQ
                     TTRPGPYTSSTFPPEIGIISLGGANKSFFDDFAYYVRGGYSGSGSDGSGTVIQY"
BASE COUNT          446 a          416 c          355 g          463 t
ORIGIN      
        1 tgtgaaagtt ctgtttatta aatagtatta aatagaaccg tccccttttt cgtataagcc
       61 tataatcttt cttttatcca tagagttctg acattcaacg tctccacttt ttccgagtca
      121 cctcaatagt tttatccgat tacgaaaaaa cactcacttg aaaactaata ctgaatgact
      181 gttccgctcc cgtcggaacc gctaccagag tatccgcccc tgacgtaata tgcaaaatca
      241 tcaaaaaagc tcttatttgc tccaccaagc gaaataatgc caatctccgg tggaaatgtg
      301 gaactcgtat aaggtcccgg cctggttgtt tgtggattca caatggccgt tcggataatc
      361 gcccctgctt ctttaccaat tcccataaca cggatggtcg gatccactgt ttgatttagg
      421 ctgctactcc actggaccag cgaaaatgag tcgtccgtat ctgtccatga ctgaacatca
      481 gtaatgggcc agttgatatt tcctcgaatt tgaccatgac ggacgtcatc ggcaccgctt
      541 gtctccagag gggttttatt ggatgaatgg tctgttgtat cggtgtagaa gacccatata
      601 taattatcac gcggacgata cccccaaccc ggatccgtat cgaatggtaa ggtccgattg
      661 agtatggtgg tcgaagtcga aaatgtacca ttaacattat tgagcagtag taccaccttc
      721 ccatcgacat cgtttatttt ccaaaaaact cttgctgagt cattgctgcc tgcccccgag
      781 atgatggtat cgccaatgtt aatgagtggc gcggaatttg tcttcaactt caaagatgcc
      841 gcttcgacca accgcaccat gagagttgac caatttttaa catgtcctga agcatcgaca
      901 ataaagtctt tccctgtttg atccagcagc ttgtaagaca accattcacc gtcgccgttg
      961 ccttgattac ttcctctgac ccacagaaca atcataggct tgtcttcaga aaacagtttc
     1021 caatagctat cattagtggg tttaatactg ttgcctaatg taattgcctg aatacactcg
     1081 taataggcat tattataact cacaacaaca ccgacagaat attctgtacc tgatatccat
     1141 ggttgccaag acgatggtgt gactgctgac agcattgtat ttcggacaag ggaaatgcct
     1201 aagaactgct gttgctgacc ctgagcttgc atcccccgga aaacgagacc attagcgtat
     1261 gtatccgggg gattgcttat agtaccagaa gaaagagtac ccgtggcaat cttaatttgg
     1321 aggtcgtaac tcgaaaaacg atccattgcc tgccagttca gatagaaggg attggaagtg
     1381 gccgaaggtg caaacacata ggcttctgca tttgctccac taccctgatc tttcgtcacg
     1441 gccaccgcct gattaccatc cacatcgact gttgcaaatc ccattgagcg aggctgcaca
     1501 ttaccgggca gtgtagtcaa gtcaataatg ccatcaccct gcccgggcgt agtaccgcta
     1561 ccgcctgttt gatttgctgg ttgcactctg taagcgatta gccgctctgc cttcatgaaa
     1621 ccggtgccaa cggtgccaat ggaagaaaaa gtaatagtgt ccggattgcc gctctgcagc
//
LOCUS       DAOSGL010000383         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_76643_length_1640_cov_3.326814, whole genome shotgun sequence.
ACCESSION   DAOSGL010000383 DAOSGL010000000
VERSION     DAOSGL010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76643_length_1640_cov_3.326814"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..>1640
                     /locus_tag="PKY68_09415"
     CDS             211..>1640
                     /locus_tag="PKY68_09415"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOH25285.1"
                     /translation="MKNFFLISALLTTLFLQALTASTVPFRFRHITVEDGLASNTVRS
                     IWQNDRGIMWFGTGQGLCSFDGINIRLYPIVTDEEPSGSNKYVYAMMQDKNGLLWVGT
                     DEGVYKYDYEKNEFNFFDRETENGERIENIVNNMVEDKDGNIWFSSFERGVYRYSPGS
                     GRLKRFPMEAFQGRVDYVFVDSDNAVWLCSTAARSTLWRLDRAEDEFKVFPFRLLDGP
                     ADFQAVVMTEDVKGTLWLGLWRGGLLSLDKHSGIVRRHLHGNVPAGSLVHIHSLNEIE
                     PNQLLIGSDEGLMWYNTLSSEYKLYLPKATDATSISNKFVYPIFKDKEGGLWIGTYYG
                     GINYVSPNNGQIESYACRAGKMEEAKIISFFCEDKNSNIWIATDDDGLFKYEPGSEKF
                     TQYLPERNRNSISYHNVHALCPDGDKLWIGTYTGGLNVLNLKTGRFSYYNHVVDDSTT
                     IDGSSIYSIFKDGEGRIWVTSMRGVNR"
BASE COUNT          450 a          335 c          404 g          451 t
ORIGIN      
        1 tgattttttt cataagatgt tgattttcac ctattaaagg ttggttaatc tcttgttgtc
       61 tgtacaaaag taaacatttt cgtctgagtt gctttggcat aatcatccat tttactgtaa
      121 caattgtcca aagaatccaa tcaaattggt gcagaaaaca gctcgaaagg caattttatt
      181 atctttgtcc actattaaat ccggcttacg atgaaaaact tttttttgat atcagctttg
      241 ttgaccacct tgtttttaca agccctgaca gcttcaacgg ttccctttcg ttttcgtcac
      301 ataacggtcg aagacggcct ggcatcgaat acggtacgca gtatatggca aaacgaccgg
      361 ggaattatgt ggtttggcac cggccagggt ttgtgcagct ttgacgggat taatatcaga
      421 ctttatccga tagtaacgga tgaggaaccc tccggtagca ataaatacgt ttacgccatg
      481 atgcaggata aaaacggttt gctttgggtg ggtaccgacg aaggagtgta caaatacgat
      541 tacgaaaaga atgagtttaa tttttttgac cgggaaacag agaacggcga acgcattgaa
      601 aacattgtca ataatatggt cgaggacaaa gacggcaata tctggttttc gagttttgaa
      661 cggggagtct atcgctacag tcccggcagt gggcgtttga agcgtttccc catggaggcc
      721 ttccagggaa gggtcgatta tgtttttgta gattccgaca atgcggtctg gttgtgcagc
      781 acggcagcaa ggtcgacact ttggcggctg gatagggcag aagatgaatt caaggttttc
      841 ccctttcgtc ttttggatgg gcccgccgac ttccaggctg tagtgatgac cgaagatgtt
      901 aaaggaactt tatggttggg actttggcgg ggtggtttgc ttagtttgga caaacacagc
      961 ggcatagtcc gccgtcatct gcacggtaat gtacctgccg gtagcctggt gcatattcac
     1021 agtctgaacg aaattgaacc caatcaatta ttgatcggct ccgacgaggg tctgatgtgg
     1081 tacaacacgc tgagttccga atataaactt tatctcccaa aggcaaccga tgccacttcg
     1141 atttccaaca aatttgttta tcctatattc aaagataagg aaggcgggct atggataggc
     1201 acttattacg gcggaatcaa ttacgtatcg ccaaacaatg ggcagatcga aagctacgcc
     1261 tgcagggccg gcaaaatgga agaggccaaa ataatcagtt ttttctgcga agataaaaac
     1321 tccaatatct ggatcgccac cgacgacgac ggtttgttta aatatgagcc cggcagtgaa
     1381 aagtttactc aatatcttcc agagcgcaat cgcaacagta tttcctatca caacgtgcac
     1441 gcgctttgcc cggacggaga taagttgtgg atcggcacct atacgggagg cttgaatgtg
     1501 ctcaatttga aaacaggccg gttttcgtat tataatcatg ttgtagacga cagtaccacc
     1561 atcgacggca gcagtattta ttctattttc aaagacggag aaggacggat ctgggtcacc
     1621 agtatgcggg gagtaaaccg
//
LOCUS       DAOSGL010000384         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_77314_length_1630_cov_4.198095, whole genome shotgun sequence.
ACCESSION   DAOSGL010000384 DAOSGL010000000
VERSION     DAOSGL010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77314_length_1630_cov_4.198095"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKY68_09420"
     CDS             <1..365
                     /locus_tag="PKY68_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_091910270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOH25286.1"
                     /translation="TITRNVKEHVESTADLTTDDSTSYTKLKEHVHSHTASVIPHEDL
                     SNVLPWVHTAISNAKRQLLGVYYKIKPEYLQYYLNQFCYKFNRRYFGENQFERLLIAA
                     VTYAPDFKSRIYNRNYCG"
     gene            complement(526..599)
                     /locus_tag="PKY68_09425"
     tRNA            complement(526..599)
                     /locus_tag="PKY68_09425"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(563..565),aa:Ala,seq:ggc)
     gene            751..1323
                     /gene="ruvC"
                     /locus_tag="PKY68_09430"
     CDS             751..1323
                     /gene="ruvC"
                     /locus_tag="PKY68_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOH25287.1"
                     /translation="MSTQPIIKERIILGIDPGTTVMGYGVIRVLNNKPEFLSMGVLQL
                     DKYEDHYLRLQKIFERCIGLIDEYLPDELAIEAPFFGKNVQSMLKLGRAQGTAIAAAL
                     SRDIPIFEYAPLKIKMAITGNGRASKEQVADLLRRFLGISPDHLLPQLDATDGLAAAF
                     CHYLQSSRPQSEKPYKSWKDYIRKNPGSCH"
BASE COUNT          518 a          375 c          316 g          421 t
ORIGIN      
        1 gtacaattac aaggaatgtc aaagagcacg ttgaaagcac ggcggacctg accaccgatg
       61 actcaacttc ttacactaaa ttgaaagagc atgtccattc acatacggca tccgttattc
      121 cacacgagga tctttccaat gtgctgccct gggtccatac cgcgatcagc aatgccaaac
      181 gacagctctt gggcgtgtat tacaagataa aaccggaata cttgcaatat tatctcaacc
      241 agttctgtta taaattcaac agacgttact tcggggaaaa ccagtttgaa agactgttga
      301 tagccgctgt aacgtatgct cctgatttca agtcaagaat ttacaatagg aactattgcg
      361 gataatcatt ttagctgatt aataaaagtc gtagataaaa aatattagtc cgactaaggt
      421 agtaaaaaaa ctgaaataca aaatcaatat agcaaaatct agttgctcgt aggtcgcatg
      481 aagtaaaaaa aagagctgtc tcattttgag acagctccaa tgcggtggag catagcggac
      541 tcgaaccgct cgcctcaaca ctgccagtgt tgcgctctag ccagatgagc taatgcccct
      601 tagatcaaac cggcggcaaa gatagtataa atttcaattc caataaacaa agagtataaa
      661 aaaatcatcc ctgctcctat ttatttaagc tcaaaccatg aaaattgtca atttggtttt
      721 acctttgcat acagcatttc actcaacagc atgtcaacgc agcccatcat caaagaacgc
      781 attattttgg ggatcgaccc cggaaccacg gtgatgggct atggtgtcat cagggtgctc
      841 aacaacaaac ccgaatttct cagtatgggc gttttgcaac tcgacaaata cgaagaccat
      901 tacctgaggc tccaaaaaat ctttgaacga tgtatcggct tgatcgacga atacctgccc
      961 gacgaactgg ccatcgaagc gccttttttc ggcaaaaacg tgcaatcgat gctcaaactg
     1021 ggccgggctc agggtaccgc tattgcggca gccttatcca gagatatccc catttttgag
     1081 tatgcccctt taaaaatcaa gatggccatt acaggcaacg gccgggcttc caaagaacag
     1141 gtcgccgatt tactaagacg tttcctcggt atatcccccg atcacttgtt gcctcaactg
     1201 gacgccaccg acgggctggc tgccgccttt tgtcattatc tgcaaagcag ccggccccaa
     1261 agcgaaaaac cgtacaaaag ttggaaagac tacattcgaa aaaatcccgg cagctgccac
     1321 taaactacca aatcaaaata aattaaaact tgcttgtcaa aataaaaact gtcactacct
     1381 ttacactatc caaaaaatta gggctgtaaa aaaattgaac atgttggtgt attgttaaat
     1441 tgttttactt aaccgaattt gtgaacatct aaaaaaacca gaaaaataaa tgaaattcgt
     1501 cacaccgtat ttgttgcgat ttacgttggc tgccgccata ttaaccgtag tttttcgata
     1561 ttttttgagt tatggaattg aagtcgactc aggagccatc atcatcgttt cggcttcact
     1621 ttacggattt
//
LOCUS       DAOSGL010000385         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_77952_length_1622_cov_2.864071, whole genome shotgun sequence.
ACCESSION   DAOSGL010000385 DAOSGL010000000
VERSION     DAOSGL010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77952_length_1622_cov_2.864071"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1622)
                     /locus_tag="PKY68_09435"
                     /pseudo
     CDS             complement(19..1622)
                     /locus_tag="PKY68_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
BASE COUNT          406 a          399 c          370 g          447 t
ORIGIN      
        1 ctccccgagc gcttgtcaaa tttcttcgct gaaatgcgga gcaaaggggg ccaaaaggat
       61 gaccaagggt ttaattattt ctcttttgtt gcatttcatt gccgacaatt cattgacaca
      121 gatcataaaa gcgctgacgg ccgtattgaa cgaaaaatgt tcaatgtccc aattcagttt
      181 cttaatcaac ttatgaagcg atttcagttc ctgtttgccg ggagctgcat cgctgaaact
      241 gaactcatct tccgaaacac aaagattcca tagcttttta aggaaacgat gcacgccgtc
      301 aatcccgttg gtatcccagg gcttagattg ctccagaggg cccaggaaca tttcgtacaa
      361 cctcagggtg tcggctccgt atttctcgac gatctgatcg ggattgacca cgttatacat
      421 cgacttggac attttttcga tggcccagcc acaaatgtat ttgccgtctt ccaaaataaa
      481 ctcggcatct tcgtagtcgg gactccagtt gcgaaaacgc tccaaatcaa gcacgtcgtt
      541 gtttacaatg tttatatcca cgtgaatggc agtgtattcg tacttgtctt tgagattgtt
      601 ggaaacaaaa gtgttgctgt ctttgattct gtacacaaaa ttggagcggc cttgaatcat
      661 accttggtta acgagcttct tgaaaggttc atcttcacat accaccccca gatcgtacaa
      721 aaatttattc cagaaacggc tgtacagcaa gtgtccggtg gcgtgttccg tacccccgac
      781 gtatagatcg acctgtctcc agtaagtatt ggcttctacc gaaaccaggg cctcttcgtt
      841 atgcggatcc atgtaacgca aataataggc cgatgaaccg gcaaaacccg gcatggtgtt
      901 caattccaaa ggataaccct cttcggtttt ccatcctttg gcgtgaccca gcggaggttc
      961 ccctttttcg gtgggcagaa aattatccac ttcgggcaat tccagaggca aagagctttc
     1021 gtccaacact tgaggcaaac cgtctttgta atagaccgga aaaggctctc cccagtaacg
     1081 ttgacggctg aatatggcat cgcggagccg gtaatttact ttgacccgtc ccagttgatt
     1141 tttagtaagg taggcttttg ttttttcaat ggcttccggc acactcaatc cgtttaaaaa
     1201 gccggaattc ttcataatac cttctttggc atcgaaactt tcttccgaaa tatcgcaacc
     1261 ctcaatcaag gggataatgg gcagatcgaa gtgttttgca aaagcatagt cccggctgtc
     1321 gtgtccggga acagccataa tggctccggt gccgtagccg gccagcacgt aatcgcttat
     1381 ccaaatgggg atttcggctc tcgacaacgg atttatggcg taactgccgg taaacacacc
     1441 gcttacccgg cggtcggcta tgcgttcccg ttcggtgcgt ttcttcactt tctgaagata
     1501 ggcgtccacg ccagccttat attcgggttt tgtaaggctc gccactaatt cgctttcggg
     1561 agccaatacc ataaatgtta ctccgaaaat agtgtcggca cgggtggtaa aaatggggat
     1621 gg
//
LOCUS       DAOSGL010000386         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_78013_length_1621_cov_4.024904, whole genome shotgun sequence.
ACCESSION   DAOSGL010000386 DAOSGL010000000
VERSION     DAOSGL010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78013_length_1621_cov_4.024904"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKY68_09440"
     CDS             <1..490
                     /locus_tag="PKY68_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HOH25288.1"
                     /translation="AKAPELFRFGMQLQMPLLMDRIEYYGRGPIENYSDRNNSTDLGI
                     YKQTVEEQFYPYIRPQENGNKTDIRWWKQTNAGGRGIKIRSVAPFSASALNYSIESLD
                     DGPSKGQRHSQQIPKVDYTNLCIDKVQMGLGSVNSWGAMPRDEYRLPHQDYEFQFVME
                     VR"
     gene            complement(590..>1621)
                     /gene="holA"
                     /locus_tag="PKY68_09445"
     CDS             complement(590..>1621)
                     /gene="holA"
                     /locus_tag="PKY68_09445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOH25289.1"
                     /translation="IRIYMAKKTENAQEVIREIEAGKRYPIYFLMGEEPYYIDEISNR
                     LLKLVLTESQQDFDQRILYGKDLSVAQLINEARRYPMLAPYQVILVREAQMIRNFEEE
                     FLLYVQQPMPSTILIINYKYKSLDKRRKLYSALEQSAILFESKKMYDNQIPAWITRFL
                     SEQEIQIEAKAAQMLADFLGTDLGRIVGELEKLKLLLKTKPYKITAAMVELNIGVSKE
                     YNNFELIDAIKQKDQFKAQQIAQYFARNPKNNAMVVTLIVLFDYFSKLLMYHYLPDKS
                     SSNVASQLGIPPFLVKNYQQGAQHYNANKVVRNISLIRHYDARLKGFEQAALPESELL
                     RELLCLLMA"
BASE COUNT          446 a          352 c          363 g          460 t
ORIGIN      
        1 tgcaaaggct ccggaattgt tccgctttgg catgcaactg caaatgcctt tgctaatgga
       61 cagaattgag tactacggcc gtggtccgat cgagaattat tcggacagga acaattctac
      121 cgatttgggt atttacaaac aaacggttga ggaacagttt tatccctaca tcaggcctca
      181 ggaaaacggc aataaaaccg atatccgctg gtggaaacaa acaaatgccg gaggcagagg
      241 tataaaaatc cgctcggtcg cccctttctc ggcctcggca ctgaattatt ccatcgaatc
      301 gctcgacgac ggtcccagta aaggacaaag acactcgcag caaataccca aggtggatta
      361 taccaacctt tgcatcgaca aagtacaaat gggtctgggt agtgtaaaca gttggggagc
      421 tatgcccaga gacgagtatc gcctgcccca tcaggattac gaattccaat ttgtaatgga
      481 agtccgttaa aaaattagtg taaatgctga agagggtgac taaaaagtca tcctcttttt
      541 ttctgttcat tcagtatccg cctgcaaagt caggcaacca tcgggaagat tatgccatca
      601 acaggcaaag caattcacga agcaattccg attcaggcaa ggccgcctgc tcaaatccct
      661 tgagcctggc gtcgtaatgc cttatgagac ttatgtttcg cacaacttta ttcgcattgt
      721 aatgttgagc tccctgctga tagtttttta ctaaaaatgg aggaattccc agttgtgaag
      781 caacattgga agaggatttg tcgggcaggt aatggtacat cagcagtttt gaaaaataat
      841 caaacaaaac aatcagtgta accaccatgg cattgttttt gggattccgg gcgaaatatt
      901 gcgcaatttg ttgggctttg aattggtctt tttgcttgat ggcatcgata agttcaaaat
      961 tgttatactc cttgctgacg ccgatattta gctcgaccat ggccgccgta atcttataag
     1021 gcttggtttt gagcaataat ttcaattttt ccaattcccc cacaatgcgt cccaaatcgg
     1081 tgcccaaaaa atcggccagc atttgggcag ctttggcttc gatctgtatc tcctgctccg
     1141 ataaaaagcg ggtaatccag gcgggtatct ggttgtcgta catttttttt gattcaaaca
     1201 aaatggccga ttgctctagg gctgaataga gttttcttct tttgtcgagg cttttgtatt
     1261 tatagttaat aatcaaaatg gtcgaaggca tcggttgttg aacataaagt aagaactctt
     1321 cttcgaaatt gcgtatcatc tgggcttcgc gcaccaatat cacctggtaa ggggctaaca
     1381 taggataacg gcgggcttcg ttaataagct gagcaacact gagatctttg ccgtaaagta
     1441 tgcgctggtc gaaatcttgc tgactttcgg tcaaaacaag tttgagcaga cgattgctta
     1501 tctcgtcaat gtaatagggc tcctctccca tcaaaaagta gattggatac cgcttgccgg
     1561 cttctatttc ccgaatgact tcctgtgcat tctccgtttt tttggccata taaatcctga
     1621 t
//
LOCUS       DAOSGL010000387         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_79302_length_1604_cov_3.223370, whole genome shotgun sequence.
ACCESSION   DAOSGL010000387 DAOSGL010000000
VERSION     DAOSGL010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79302_length_1604_cov_3.223370"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PKY68_09450"
     CDS             <1..672
                     /locus_tag="PKY68_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOH25290.1"
                     /translation="ARALGQRFGKTELWYVVRAGSEAELVAGFNKELSREEYLRRIAD
                     DSIEEVLLRHPVQAGDVFMMPPGRVHSIGKDILLAEIQQSSDITYRIYDFGRKDDRGN
                     TRPLHTELAAQCLDYKLYSEYKTSYPKLLNQAVELNRNKYFRVNRLELKAQTPLLEIE
                     RNPAAGKSFIAYMCIEGALELHCSNGSYGRLTRGRTLLIPAAAGSCRLQCKESALVLE
                     IVADC"
     gene            complement(784..>1604)
                     /locus_tag="PKY68_09455"
     CDS             complement(784..>1604)
                     /locus_tag="PKY68_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOH25291.1"
                     /translation="GFGSSREPRVNRGSNLRIKVKLNLKEVANGTEKKIKVKKQVACA
                     HCKGSGAEGGSAYSTCNTCNGRGSVTRVTNTILGRMQTSSVCPDCQGEGKIITNKCSH
                     CHGEGIESKDEIINIKIPAGVEDGMQLSLSGKGNAARRGGINGDLIVVIETIDHPELI
                     REGNHLIHNLLLSFPTASLGGTVEIPTVDGKVKVKIDPGTQPGKILRLRGKGLPSVNQ
                     YGTGDLLVNISVYIPENLSREERRIIESMEQSDNFKTSPSLSKKIFTKFRNLFD"
BASE COUNT          364 a          386 c          419 g          435 t
ORIGIN      
        1 gcccgtgctc tgggacagcg tttcggcaag accgaacttt ggtatgtggt acgtgccggg
       61 tctgaggccg agctggttgc gggcttcaac aaagagctga gccgcgaaga atacctgagg
      121 cgtatcgccg acgacagcat cgaagaggtt ttgcttcggc atccggtgca ggccggcgat
      181 gtttttatga tgcctcccgg cagagtccat tccataggca aagacatcct attggccgaa
      241 atacaacaaa gctccgatat cacttaccgg atttacgatt ttggccgcaa ggacgaccgg
      301 ggaaatacca ggccgctgca tactgagttg gcggctcaat gtttggatta caagctttat
      361 tccgagtaca agacaagcta ccccaaacta ttgaaccagg cggttgaatt aaatcgaaat
      421 aagtatttta gagttaacag gttggaatta aaagcccaaa cacctctcct cgaaatagag
      481 cgtaatcctg ctgccgggaa atcgtttata gcttatatgt gcatcgaggg agccctggag
      541 ctgcattgtt caaacggcag ttacgggcgt ctgacccggg gccggacctt actcattccg
      601 gcagcggcag gctcttgccg cctgcaatgc aaggagtcgg cgttggtttt agagattgtt
      661 gccgattgct gagttaaact tcccaggaaa gtgcaagaat tttcagagaa tgagttaaac
      721 ttctcgagaa aagtaagaat tttccgaaaa tgagctaaac ttcccgggaa aactttcgga
      781 agcttagtcg aaaagattgc gaaacttggt aaaaatcttt ttactcaggc tgggtgaggt
      841 tttgaagttg tcggattgct ccatactttc gattatcctc cgttcctcgc ggctcaggtt
      901 ctcgggaata tacacgctga tgttgaccaa aaggtcgccc gtgccgtatt gattcaccga
      961 aggcaagcct ttgcctctga ggcgcagtat cttaccgggc tgtgtgccgg gatcgatttt
     1021 tactttcact ttgccgtcga cggtggggat ttcgacggta ccgcccaggc tggccgtggg
     1081 gaaactcaac aaaagattgt ggataagatg attgccctca cggatcagtt ccggatgatc
     1141 tatggtttcg attactacta tcagatctcc gttgatgccg ccacggcggg cagcgttgcc
     1201 cttgccgctt aaggaaagtt gcatgccatc ctccactccg gccggaattt tgatgtttat
     1261 tatttcatct ttgctttcta tgccttcgcc gtggcaatgg ctgcatttat tggttataat
     1321 tttaccttcg ccctggcagt cggggcaaac cgacgatgtt tgcattctac ccagaatggt
     1381 gttggttacc ctggttacgc tgcctctgcc gttgcaggtg ttacaggtgg aataggcaga
     1441 gccgccctcg gctccgctgc ctttgcaatg agcgcaggca acctgctttt tgactttgat
     1501 ctttttctcg gttccgttgg cgacctcttt gagattgagt ttaactttga ttcgtaaatt
     1561 ggaaccccta ttcactctgg gttcgcgtga actgccaaag ccac
//
LOCUS       DAOSGL010000388         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_80075_length_1594_cov_2.721248, whole genome shotgun sequence.
ACCESSION   DAOSGL010000388 DAOSGL010000000
VERSION     DAOSGL010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80075_length_1594_cov_2.721248"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKY68_09460"
     CDS             <1..354
                     /locus_tag="PKY68_09460"
                     /inference="COORDINATES: protein motif:HMM:NF019903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH25292.1"
                     /translation="VKNVDFERCKQVVDNSIQVKERPISVELIENEVCKYYNLEPQLI
                     QTRSKKQEVAHARQIAMYLSKKYTDSSYARIGELLGKRNHATVIHACKTVNNWIETDK
                     GLRSDIQKIEKILLR"
     gene            complement(413..1150)
                     /locus_tag="PKY68_09465"
     CDS             complement(413..1150)
                     /locus_tag="PKY68_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH25293.1"
                     /translation="MDSMKKRATVRAYSQQDISPELLDELLEIACRSSNTGNMQAYSI
                     VVSRDPERKKALAACHFNQPQVVNAPVVLTFCADFNRFSKWCAQRNAQAGFNNVQALT
                     YASIDAVIVAQTFCVAAEEKGLGICYLGTTTYLAKEIIELLELPPLVFPVTTVSVGYP
                     LEPPKQCERLPQEAVVHSETYRDYTPADIDRLHLAKEQLPENRHFVEINHKENLAQVF
                     AEIRYTKKDNEHFSDTLLRAVKKQGFC"
BASE COUNT          468 a          324 c          343 g          459 t
ORIGIN      
        1 gttaaaaacg ttgattttga gcgctgcaaa caggtggtgg acaactccat tcaggtaaaa
       61 gaacgcccca tttcggtgga acttatcgaa aacgaggtat gcaaatacta caatttggaa
      121 ccccaactga tacaaacccg ctccaaaaag caggaagtgg cccacgcccg gcaaattgcc
      181 atgtaccttt ccaaaaaata caccgacagt tcctatgccc gcataggcga gctgctgggc
      241 aagcgcaatc acgctaccgt gatccatgcc tgcaaaacag taaacaactg gatagagacc
      301 gataaaggtt tacgcagcga tattcaaaaa atcgaaaaga ttttgttacg gtaaatatcc
      361 gagagtttcc cagtctaaag caaacttcag gaattaatct taaagcaggg cttcaacaga
      421 aaccctgctt ttttactgcc cggagcagtg tatcggagaa atgttcgttg tcttttttcg
      481 tataacggat ttcggcaaat acctgagcca ggttttcttt atgattgatt tcaacaaaat
      541 gccggttttc gggcaattgc tctttggcca ggtgcagacg gtcgatgtcg gcgggcgtat
      601 aatcgcggta ggtttcggaa tgaacgacag cctcctgtgg aagacgttcg cattgtttgg
      661 gaggttcaag aggatagcct acactgacgg tggtgaccgg aaaaaccaaa ggcggaagtt
      721 ccaggagttc gattatttct ttggccagat aggtagttgt gcccaaataa caaataccca
      781 ggcctttttc ctcggcagct acacaaaaag tctgagctac gatgacagca tcaatggaag
      841 cgtaggtaag ggcctgaaca ttattaaaac ctgcctgggc attacgctgt gcacaccatt
      901 tgctaaaccg gttgaaatcg gcacaaaaag tcaaaaccac cggtgcattg accacctggg
      961 gctgattgaa atggcaggct gcaagggctt ttttgcgttc gggatcgcgg ctcaccacga
     1021 tgctgtaagc ttgcatattg ccggtattgg aagaacggca ggcgatttcc agtaattcat
     1081 ccagcagctc cgggctgata tcttgttggg aataggcacg tacagtggct ctctttttca
     1141 tcgaatccat agaattataa tttaggttgt ggtttttttc tttgcaaaga tagggtttaa
     1201 aaaaaatctt cccaattatt ttttttgtca acttgactaa ttctattttt gttgaaagtt
     1261 tttattttta tcgttatctc tttttattac tttttgttat atagcacatt atctgatact
     1321 tataagtttt cgatttatca tttgtaagaa ttaataatat attcatgttg aatttttttt
     1381 tcacttgatt atttgagcta atattgcaaa ctcaatagaa caaacccaaa acttttgcag
     1441 aagaatcaaa aatttcatca acttatataa ccacttttta tcatggaaaa aaaaatctac
     1501 acacgggagg aggcttttga tgcctcgctt gagtatttcc aaggcgatga actggctgcc
     1561 agagtctggg taaacaaata cgccataaaa gatt
//
LOCUS       DAOSGL010000389         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_80218_length_1592_cov_3.052700, whole genome shotgun sequence.
ACCESSION   DAOSGL010000389 DAOSGL010000000
VERSION     DAOSGL010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80218_length_1592_cov_3.052700"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..>1592
                     /locus_tag="PKY68_09470"
     CDS             376..>1592
                     /locus_tag="PKY68_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25294.1"
                     /translation="MKRFYPAIITFLILALPIQARKSWEVREKGISAASVEKILADTE
                     NWKQTIKDGVEDTRATNLNAFTGEVKAQGEILPEFSELYFANVQANTFEVRWQEGNAY
                     FRMNKDVQTFTIKKLVAGQTITIITKSANSSDPRGIECKSGHCTRTAGAEKMTGAEGD
                     NINVFQVNADITDSVDVAFGPNVGGLLIYSVIVDQGDEEFDFSESKVAYLYDASTQDI
                     DSDPFFNYTKIAEVASESIDIKDFSSVDTDTLSALENYDLVVLSYGLKADHPFVAMLK
                     KQVNRVPILNLNASLNDAWGWASLENPSNAAEEGGIGTIKIPEGAEAHAIYADIDVSS
                     AEIALFFESDPALLKCNLIQAYQAVEGGLFANDSVLAVVEGPAGTFNAIHEHASANTY
                     MLIPFGPDAVVVEG"
BASE COUNT          452 a          350 c          373 g          417 t
ORIGIN      
        1 aagcatcgtg aatagccggc agttcctctt gtttacttgc atccaggtcg aaataaacgt
       61 ccactaccgg accaataatt tgcgaaatag ttcctattga tttaatcata ctcaaactgt
      121 gtcctaatat aagagggagc aatagttcgc aaaattacaa aaaaacagaa gctttgaaat
      181 tatttagaac taattttatt ataaagttgg gttgcagggt cagctaccgc tgtaagtcat
      241 gctccggttt tgtcaaagtt tccaaacaaa aattcaaaat tctgtttaat ccgtcttatt
      301 tttgtggaac attttccaat attttactaa cttgcggctc tctaaagtgc acaacaatac
      361 taaatactaa tgcttatgaa acgtttttac ccggccatca ttacattttt aatactggca
      421 ttgcctatac aggcccgcaa gtcgtgggaa gtgagagaaa aagggataag tgcggcttcc
      481 gtggagaaaa tcctggcaga caccgaaaat tggaaacaaa ccatcaaaga tggagtggaa
      541 gacacacgtg ctaccaacct gaacgctttt acgggagaag tcaaagcgca aggcgagatc
      601 ttgcccgaat tcagcgaact ctattttgcc aatgttcagg ccaatacttt tgaagttcgt
      661 tggcaggaag gcaatgctta tttcaggatg aacaaggatg ttcagacttt caccataaaa
      721 aaattggtgg caggtcagac catcaccatc attaccaaat ccgccaacag tagcgaccct
      781 cgcggcatcg agtgtaaatc gggccactgt acgcgtacgg cgggtgccga aaaaatgacg
      841 ggcgcagaag gcgacaacat caatgtcttt caggtgaatg cagatatcac cgactctgtg
      901 gacgttgctt ttggtcccaa tgtgggcggt ctgcttatct acagtgtcat tgtggaccaa
      961 ggcgacgaag aatttgattt cagcgaatcc aaagtggctt atttgtacga tgcatcgacc
     1021 caggacatcg acagcgatcc cttctttaat tacaccaaaa tagcggaagt ggcttccgag
     1081 tccatcgaca ttaaagactt tagctcagtc gacaccgata cgctgtctgc tttggaaaac
     1141 tacgatttgg tggtgctttc ttacggactg aaggccgatc atccttttgt ggccatgttg
     1201 aagaaacaag tgaatcgtgt ccccatactt aacctgaatg cttcactcaa cgatgcctgg
     1261 ggatgggctt cgctggagaa tccgagcaat gcggccgaag agggcggtat tggcacaatt
     1321 aaaatacctg agggtgctga agcacatgcc atatatgccg atattgacgt cagcagcgca
     1381 gaaattgctc tgttcttcga gagcgatccc gctttgctta aatgtaatct gatacaggct
     1441 taccaggccg tcgagggcgg attattcgcc aacgattccg tgctggcggt agtagaaggg
     1501 ccggcgggaa cttttaatgc cattcacgaa catgcttctg ccaataccta tatgttgatt
     1561 ccttttggcc cggatgcagt ggtggtagag gg
//
LOCUS       DAOSGL010000390         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_81160_length_1580_cov_3.857049, whole genome shotgun sequence.
ACCESSION   DAOSGL010000390 DAOSGL010000000
VERSION     DAOSGL010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81160_length_1580_cov_3.857049"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PKY68_09475"
     CDS             <1..679
                     /locus_tag="PKY68_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOH25295.1"
                     /translation="KAYVCSFDGSLARIDTASLEVEAYVNCGRNPDGICLTNNKIYVS
                     NSGGLDNPYFDNSVSVVDPLSFREIKKIETGLNPYQMQVDSQGDIYVVLRGNLLDIPF
                     SLCRIDSRLDSVVQVFNDIPAWNLAIHNDTAYIYHYSVQGGENSVKVFDCLTETIISD
                     QFISDGTPITRPYAIEVNPYSGDVYICDAYDYTLWGDCLCFSAEGNLKFRFESVGLNP
                     SSLVFVK"
     gene            753..>1580
                     /locus_tag="PKY68_09480"
     CDS             753..>1580
                     /locus_tag="PKY68_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOH25296.1"
                     /translation="MEKNTERSLARLCKQHFGLEISSLQEMPACGSYRRYYRIYLPAP
                     APASIIGVWNADLRENAAFVSFAQSFYRAGLPVPQIYADDPHSFVYLQQDLGDQSLFS
                     LLNSEWFPVKDKPAARFPERLFLLYKKSLSCLLDMQLAGKDCIDYSKSTPSASFHRDA
                     MHWDLNYFKYFFLRLLKIPFDEQALENDFRSLVDFLQTAPSDYFLFRDFQTANIMVME
                     DEPYFIDFQGGRRGLLQYDPASLLYDAKSYLPRNSRTELLEYYVSLLETKADVRAGDF
                     "
BASE COUNT          396 a          345 c          365 g          474 t
ORIGIN      
        1 taaagcttat gtctgttctt tcgacggcag cttagccaga atcgataccg ccagcctcga
       61 agtagaagct tatgtgaatt gcggacggaa tccggacgga atctgcctga caaacaataa
      121 aatttatgtg tccaattccg gcggcctcga caatccctat ttcgacaaca gcgtttcggt
      181 ggtcgatccg ctcagctttc gtgaaataaa aaaaatcgaa acagggctga atccatacca
      241 gatgcaggta gacagtcagg gtgatattta cgtggtgctc cggggtaatt tgttggatat
      301 tccttttagt ttatgccgga tagatagccg cttggattcg gttgtgcagg tatttaatga
      361 cattccggcc tggaatctgg ctatacacaa cgacactgcc tatatctacc attattcggt
      421 acaaggcgga gaaaactctg taaaggtctt tgattgtctg acagaaacaa taatttcaga
      481 tcaattcata tcggacggaa ctcctatcac acgcccctat gccatagagg taaatcccta
      541 ttccggcgat gtgtacatct gcgatgccta cgattatacc ctttggggag attgtttgtg
      601 tttttctgca gagggaaatt taaagtttcg attcgagagc gttggcttga atcccagcag
      661 tttggttttt gtcaaataaa ccgcggactg ctgttctgct cgaaaagaat agctattttt
      721 gttgccggac gatgctccct gaaccattta gtatggaaaa aaacactgaa agaagtctgg
      781 ctcgtttatg taaacaacat tttggcctgg aaatatcgag cttgcaggaa atgccggcct
      841 gcggatccta tcgtcggtat tatcgtattt atttgccggc tcctgctcca gccagcataa
      901 ttggcgtctg gaacgccgat cttagggaaa atgccgcctt tgtttcgttt gctcaatcct
      961 tttaccgggc cggtttgcct gtgccccaaa tttacgcgga cgatccacat agctttgtgt
     1021 atttacagca ggatttgggc gatcaaagtt tattctcgct tttgaattcc gaatggtttc
     1081 ctgtcaaaga caaaccggct gcccgttttc ccgaaagact tttccttttg tataaaaaga
     1141 gcctgagttg cctgctcgat atgcaattgg ccggaaaaga ctgcattgac tattccaaat
     1201 ccacgccaag tgcttctttt cacagggatg ccatgcactg ggacctgaac tattttaagt
     1261 atttcttttt gcgtttgctt aaaatacctt ttgacgaaca ggccctggaa aacgatttcc
     1321 gcagcctggt ggattttttg cagacggctc cttcggatta ttttttgttt cgcgatttcc
     1381 agacggccaa tattatggta atggaggatg agccctattt tattgatttc caaggagggc
     1441 gcagaggttt gttgcaatac gatcccgctt cactcttgta cgatgccaaa tcgtatttgc
     1501 ccaggaactc caggacagaa ttattggaat actatgtttc tttgttggaa acaaaagccg
     1561 atgtcagggc gggagatttt
//
LOCUS       DAOSGL010000391         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_81587_length_1575_cov_2.765789, whole genome shotgun sequence.
ACCESSION   DAOSGL010000391 DAOSGL010000000
VERSION     DAOSGL010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81587_length_1575_cov_2.765789"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PKY68_09485"
     CDS             complement(<1..888)
                     /locus_tag="PKY68_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOH25297.1"
                     /translation="MKKITFTFIFMVSVLLMSLSALAQKASVNGLVVDEKGVPIPMVS
                     FAVKGTTIGTFSDLDGKYSIANLDAGRHIIVAYMMGYKKEEITLELKAGQALTQNFTL
                     KEEAYLLNETVVVGYGVKQKRDVTGSIETIKAKDIAQSSGTSSFEQAIQGRASGVQVT
                     AANGVAGAPVKINIRGTSSISAGSEPLYVVDGIPIITGDLSSGALGTGTNALSDLDPK
                     DIESMEVLKDAASAAIYGSRGANGVVIITTKKGKAGKTKFDVNFSSGIVRETNRLKLI
                     SAEEQLSLRDKYREELGFAP"
     gene            1093..>1575
                     /locus_tag="PKY68_09490"
     CDS             1093..>1575
                     /locus_tag="PKY68_09490"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOH25298.1"
                     /translation="MKFQRQSGILLHPTSLPGNQGIGTFGKEAYDFIDFLVEAGQKLW
                     QILPLGPTGYGESPYQCFSSIAGNPLLINLEKLQEEGWLNKSDIEDHSGKLARYVDFD
                     KVRKETLPLLKKAFVAFKPKAKNNKDYKTFCTQKAVWLDDFSLFVALKEEFRGKPWNL
                     W"
BASE COUNT          431 a          336 c          344 g          464 t
ORIGIN      
        1 gggtgcaaac cccaattctt cgcggtattt atcgcgcagc gacaattgtt cttctgccga
       61 aatcagcttc aaacggtttg tttctctgac aatacccgaa gaaaaattta cgtcgaattt
      121 agtttttcct gctttacctt ttttggtggt gataataacc acgccattgg ctcccctcga
      181 tccatagata gctgccgagg cggcatcttt cagcacctcc atagattcta tgtcttttgg
      241 gtcgaggtcc gagagggcat tggttcctgt tcccagtgcg cccgaactca ggtcgccggt
      301 gataatcggg atgccgtcaa caacgtagag cggctcgctg cctgccgaaa tggaactggt
      361 acctcgtata ttgattttta caggtgctcc agccacgccg ttggccgcag taacctgcac
      421 tccggaagcc ctgccttgta tggcctgttc aaagctcgat gtacccgacg attgggctat
      481 gtctttggct ttgatggtct cgatagagcc ggtaacgtcc cttttttgtt ttaccccgta
      541 accaaccact acggtctcat taagcaggta ggcctcttct tttaaagtaa aattttgtgt
      601 caaggcctgg ccagctttta actctaaggt tatttcttct tttttatacc ccatcatata
      661 ggccacgatg atgtgcctgc cggcgtctaa gttggcaatg ctgtacttac cgtccaagtc
      721 ggaaaatgtt ccaatggtgg tgcctttcac agcaaaagaa accattggaa taggcacgcc
      781 tttttcgtca accaccaatc cgttgacgga ggctttttga gcaagggcgc tcaaactcat
      841 caacagaacc gataccatga aaatgaaggt aaatgtgatt ttcttcataa aaaatttttt
      901 ataacaaatt tgtaacacct gaaaatatta aggggcaaat gtattaaata ttgtaattaa
      961 tgaaaaaaaa tgcatgaaat tgtattgaaa aaatcgtgca attaacactt tcaacttgcc
     1021 ggaaaatatt aattttacac tgcagttcag aatatttgaa ttttaaaata taatatattt
     1081 aatattgtta atatgaaatt tcaaaggcaa agcggaattt tattgcatcc tacttcactc
     1141 cccgggaacc agggcatagg tacttttggc aaagaagcct acgatttcat tgattttctt
     1201 gtggaagcag ggcagaaact ctggcagatt ctaccgctgg gtccgacagg ttatggcgaa
     1261 tcgccttacc agtgtttttc atccatagcg ggaaatccgt tgctgataaa tctggaaaag
     1321 cttcaggagg agggctggtt aaacaaatct gacattgaag atcattcggg taaactggca
     1381 cgctatgttg atttcgataa agtcaggaag gagactctgc ccttgctgaa aaaagctttt
     1441 gttgctttta agcccaaagc aaagaataat aaggattata aaacattctg tacgcaaaag
     1501 gcggtgtggc tcgatgattt ttctttgttt gtagccctga aagaagagtt caggggtaag
     1561 ccctggaacc tgtgg
//
LOCUS       DAOSGL010000392         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_81880_length_1571_cov_2.945251, whole genome shotgun sequence.
ACCESSION   DAOSGL010000392 DAOSGL010000000
VERSION     DAOSGL010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81880_length_1571_cov_2.945251"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1571)
                     /locus_tag="PKY68_09495"
     CDS             complement(<1..>1571)
                     /locus_tag="PKY68_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOH25299.1"
                     /translation="ATVPSYALYMDFQAVHRDTLNKYMKPLCRALSQKFGDLSPADFE
                     ARLREGQRRASREYLIHPQRVSYADWKEIKTFPLFSKGRYQSGLYEKKYLERQKMFGS
                     LASRTIGNIYAEFGKGGRSGLELKYDSLLRGQDGVSTRQKVAGQYMNVNNKEPRDGWN
                     LRTTINIPMQDIVETALKEMLEKTEAASGTAVLMEVKTGKVKAISNLGLTSTGSYQEI
                     QNYAVSDLSEPGSTFKTIAMMAALEEGKIKPNDSVDTQKGLVRMYGQAMSDHNQEHGG
                     YGKITAAKSIWYSSNIGISTLIDKAFADNPARFVDRLYKMKINQPMDIEIPGAGYPDI
                     PHPKNKNRYWAKTDLPWMSIGYVVRIPPIYTLTYYNAIANDGCMVKPFFVEEIFRENQ
                     VHKSFGTEVLNLKICSDKTLREIRAMLDSVVEKGTGGAAKSKFFPIAGKTGTAVLSKG
                     SMGYKAGGKNYQVSFCGYFPSDRPKYSAIVVIRDPVVGYASGGVMAGGVFKEIAEQIY
                     ARELWIDLNSKRVKE"
BASE COUNT          353 a          409 c          353 g          456 t
ORIGIN      
        1 ctctttgacc cgtttactgt tcaggtctat ccacaattcc cgggcataaa tctgctcggc
       61 aatttccttg aaaactcctc cggccatcac acctcccgag gcatagccca ctaccggatc
      121 gcgtatcacc acaatggcac tgtatttggg gcgatccgaa gggaaataac cacaaaacga
      181 aacctgatag tttttgcccc ctgccttgta tcccatggaa cctttggaca aaaccgccgt
      241 tccggttttg cctgctatgg ggaaaaactt acttttggcc gcccctcccg ttcctttttc
      301 caccacgctg tccaacatcg ctctgatctc gcgcaaggtt ttgtcggagc atatttttaa
      361 attcaggacc tccgtaccaa agcttttatg tacctgattt tctctgaaaa tttcttcaac
      421 aaaaaagggt tttaccatac aaccatcgtt ggcaatagca ttgtaatagg tcaaggtgta
      481 aataggcggg atacgcacca cgtagcctat cgacatccag ggtaagtcgg ttttagccca
      541 ataacggttt ttatttttag gatgaggaat gtcgggatag cctgcacccg gaatttctat
      601 atccataggc tgattgatct tcattttgta caagcgatcc acaaaacggg caggattgtc
      661 ggcaaaagct ttgtctatca aagtagaaat gccgatgttg gaagaatacc aaatgctctt
      721 ggctgcggtt attttaccgt aacctccatg ttcctgattg tgatcgctca ttgcctggcc
      781 atacattctg accaggccct tttgggtatc gaccgaatcg ttgggcttga ttttgccttc
      841 ttccagggcg gccatcatgg ctatggtttt gaaggtggaa cccggctcac tgaggtcgct
      901 tacggcgtag ttttgtattt cctggtaaga tcccgtggag gtaaggccta aattgctgat
      961 cgcttttact ttgcctgttt tcacctccat cagtacggcg gttcccgagg ctgcctccgt
     1021 cttttcgagc atttctttca aagccgtctc aacgatgtcc tgcatgggaa tgttgatggt
     1081 cgtccttaaa ttccaaccgt cccggggctc tttgttgttg acattcatgt attggccggc
     1141 cactttttga cgggtcgaca caccgtcctg gccccggagc aaagaatcgt actttaactc
     1201 aagcccactg cgcccgcctt tgccgaattc ggcgtaaata tttcctatgg ttctcgaagc
     1261 cagagagcca aacatttttt gacgttccag gtattttttt tcatataatc cgctttgata
     1321 acggccttta ctaaacagag ggaaggtctt gatctctttc cagtcggcgt aagacactct
     1381 ctgcggatgt atcagatact cgcgactggc cctgcgctgc ccctcgcgca gccttgcttc
     1441 aaaatctgcc ggcgacaggt cgccgaattt ttgcgacaga gcccgacaaa gcggtttcat
     1501 atatttattg agggtatccc tgtgtacggc ctgaaaatcc atatacaagg catagctcgg
     1561 tactgtggcg g
//
LOCUS       DAOSGL010000393         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_86093_length_1522_cov_3.593729, whole genome shotgun sequence.
ACCESSION   DAOSGL010000393 DAOSGL010000000
VERSION     DAOSGL010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86093_length_1522_cov_3.593729"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKY68_09500"
     CDS             complement(<1..489)
                     /locus_tag="PKY68_09500"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOH25300.1"
                     /translation="MKIQDIHSQPLIEVGILSRNSIHFVLLSAYKLIGYQKDNPFRME
                     YYSLPQGSYSCAYEDGMIRFQNKLFASLGFEPESDSGRFILNNVRIGIEFHWEQEMQQ
                     CFEGALLIKTENHRLLAINRIALERYLYSVIASEMNARAHTEFLKAHAVISRSWLLAQ
                     LGT"
     gene            complement(486..>1522)
                     /locus_tag="PKY68_09505"
     CDS             complement(486..>1522)
                     /locus_tag="PKY68_09505"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOH25301.1"
                     /translation="FSLNRSLNLELKCLPQRKRSATADTSRTGIAARPCFLCAPQLPA
                     EQISLNLVFENEQITTCTKARENGYRLLVNPYPAFDRHFTIAAVDHREQSLSRELPAM
                     LALTEFMPSYLFFFNAPGAGASAPDHLHFQAVPRGCLPVERDFASWHDVYSRVLFTSG
                     QTEVFCLENFLRSAWLLKSRDPKELCALLQELLGILASSLPNTQTETSTAVNNIPQTI
                     HGGNIGSTEKFHTDVNKNPEATAVQQKPPVNLLSWYEKGLWTCVLFARSKHRPACYFE
                     RGEQQIIVSPASVEMGGLIVLIRPEDFDKINPGLISRIFAEVSLPKQAYNDISEAWKN
                     QNQHFNPRAL"
BASE COUNT          373 a          368 c          400 g          381 t
ORIGIN      
        1 agtgcccagt tgagccaaca accagcttcg ggagatgacg gcatgggctt tgagaaattc
       61 cgtgtgagcc cgggcgttca tttcggaagc gataacgctg tataaatacc tctcgagggc
      121 aatgcggttg atggccagca ggcgatggtt ttccgtctta atcagcaagg cgccctcgaa
      181 acattgctgc atttcctgct cccaatgaaa ctctatgcct atgcggacat tgttaagaat
      241 aaagcgcccg gaatcggatt ccggctcaaa acctaaggat gcgaatagtt tattttgaaa
      301 tcggatcatt ccgtcttcgt aagcgcagga atagctgccc tgaggtagag agtaatactc
      361 cattctgaaa ggattatctt tttgatatcc aataagttta taagcggaaa gcaatacaaa
      421 atggatcgaa ttgcggctta aaatacctac ttcaatcagc ggctgggagt gtatgtcctg
      481 aattttcata atgcccgggg attaaagtgt tgattctggt ttttccaggc ttcggaaatg
      541 tcgttgtatg cctgtttggg cagactgact tcggcgaaaa tccggcttat aagtccgggg
      601 tttattttgt caaaatcttc gggcctgatc aaaacaatca gtccacccat ttctaccgaa
      661 gcggggctga cgatgatttg ttgctcgccc ctttcgaaat aacaagccgg tcggtgtttg
      721 ctccgggcaa ataaaacaca ggtccagagc cctttctcgt accagctcag cagattgacc
      781 ggcggttttt gctgaactgc cgttgcttcc gggtttttat ttacgtcagt atgaaatttt
      841 tctgtcgacc cgatgttgcc cccgtgaatt gtttgaggaa tgttatttac agcagtactt
      901 gtttcggttt gtgtgtttgg cagggaagaa gcaagaatac ccaacaattc ctgtagcaag
      961 gcgcagagct ctttgggatc ccggctcttg agcagccagg ccgaacgcag aaaattttcc
     1021 aggcaaaata cttcggtctg cccgctggtg aacaagaccc ggctgtatac atcatgccag
     1081 gaggcaaaat ccctttccac aggcagacag ccccggggca cagcctgaaa atgcagatga
     1141 tcgggcgccg atgccccggc tcccggagcg ttgaaaaaaa acagatagga gggcataaac
     1201 tccgtcagtg ccaacatggc gggcagctcc ctgctcaggc tttgttcccg gtggtcaacc
     1261 gccgcgatgg taaaatgccg gtcgaaagcc ggatagggat tcaccagcaa gcggtagccg
     1321 ttttcccggg ccttggtgca ggtcgttatc tgctcatttt caaaaaccag attcagcgaa
     1381 atttgctccg ccggcaattg aggggcacat aaaaaacagg gcctggctgc aatccccgtc
     1441 cggctggtgt cggcagtggc cgaacgctta cgttggggaa ggcatttcag ctcgagattc
     1501 aggctccggt tcaggctgaa ac
//
LOCUS       DAOSGL010000394         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_87600_length_1505_cov_2.766207, whole genome shotgun sequence.
ACCESSION   DAOSGL010000394 DAOSGL010000000
VERSION     DAOSGL010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87600_length_1505_cov_2.766207"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..701)
                     /gene="ruvA"
                     /locus_tag="PKY68_09510"
     CDS             complement(111..701)
                     /gene="ruvA"
                     /locus_tag="PKY68_09510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOH25302.1"
                     /translation="MIEYIKGEIKQLTPTFAVLDKAGIGFYLQISLNTFTELQNLQEC
                     TLFVHEVIREDAYLLYGFHGSEEREAFRKLINVTGIGANTARMILSSLTVAELESCII
                     SENIHTLKSIKGIGLKTAQRLIVELKDKVIRSGMSGGEVSPASVAGNQVRQEALAALQ
                     MLGFTPSQSSKAVNTVLKEFPTLPVEQLIKAALKIL"
     gene            982..>1505
                     /locus_tag="PKY68_09515"
     CDS             982..>1505
                     /locus_tag="PKY68_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOH25303.1"
                     /translation="METNPLLLVLIGTGITFLSTVLGASFVLFFKKDINPTIQKLFLG
                     FAAGVMIAASVWSLLIPAIELAETQGKVAWTRVSVGFLAGGFFLWLLDSLLPHLHPGS
                     KTPEGLPTGWRRSTMLLLALTLHNIPEGMAVGLSFGFSGAVEQGFSLASAFALAIGIG
                     LQNIPEGMAVALPL"
BASE COUNT          386 a          358 c          336 g          425 t
ORIGIN      
        1 gcaatgtctt cctgatcctt cgattcctca gcctgagcaa agagctcttg tgccgtagcc
       61 aaaagacaaa gaacaacaag cagccatatg tactgaaaac ccttcaagaa ctatagtatt
      121 tttaaagcgg ctttgattag ctgttccaca ggtaatgtgg ggaattcttt taaaaccgta
      181 tttacggctt tagacgattg cgagggagta aagcccagca tctgaagggc agccagcgct
      241 tcctgacgca cttgatttcc ggcaaccgaa gcagggctta cttcgcctcc gctcattccg
      301 ctgcgaatca ccttgtcctt gagttccaca atgagccgct gggctgtttt gaggcctatg
      361 cctttgattg atttaagcgt atgaatattt tcactgatta tacaggattc cagttcggcc
      421 accgtaagcg aagaaagtat cattctggcc gtattagcgc ctatacctgt cacattgatc
      481 agtttacgaa aagcttcccg ttcttcggaa ccatgaaaac cgtaaagcaa ataagcatcc
      541 tcacgtatta cctcatggac aaaaagagtg cattcctgca ggttttgaag ctcggtaaag
      601 gtgttcaggc tgatttgcag ataaaagcct atacctgcct tgtccaacac tgcaaaagtg
      661 ggtgtcagct gtttgatttc ccccttgata tattcgatca taaagcaaaa atttaaggct
      721 tcaggccatt gttcgttgtt ttgaaaggct tttcaagata ataataacgc aaaaaaagcc
      781 ggaaacgtat aatttaccgg tacaaaaaag caccggagtc acaaagttag acaatcccta
      841 tcgaaattta actgctctga gcccgtccaa gccttaaaaa atgagcctga cggcttcagt
      901 ttagatgaat aatacttatc tttgcggccg ttttttatac aaaccaggcc attacaggct
      961 tgccccgaaa aaacaaataa gatggaaaca aatcccctgc ttttggtctt gatcggtacg
     1021 ggcattacct ttttgtcaac ggtattgggg gcctcctttg tcctgttttt caaaaaagac
     1081 attaatccga caattcaaaa gctttttttg ggttttgcgg ccggtgttat gatcgctgcc
     1141 tcggtgtggt ctctactgat tccagccatc gaactggccg aaacccaggg taaagtagcc
     1201 tggaccagag tttctgttgg atttttggcg ggcggcttct ttctctggct gctcgactct
     1261 ctgttacctc acctccaccc cggcagcaaa actccggaag gcttgcctac aggttggcgc
     1321 cgaagcacta tgcttttgtt ggcacttacc ttgcacaata tccccgaagg aatggcagtc
     1381 ggtttgagct ttggtttttc gggggcggtg gaacaaggtt tttcgctggc ctcggctttt
     1441 gcacttgcca ttggaatcgg cttacagaat atccccgaag gcatggctgt tgctctgccc
     1501 ttgaa
//
LOCUS       DAOSGL010000395         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_88089_length_1499_cov_3.006233, whole genome shotgun sequence.
ACCESSION   DAOSGL010000395 DAOSGL010000000
VERSION     DAOSGL010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88089_length_1499_cov_3.006233"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..>1499
                     /locus_tag="PKY68_09520"
     CDS             230..>1499
                     /locus_tag="PKY68_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOH25304.1"
                     /translation="MLDFRRVNKPDFRYSLLYRYVQFVHNHIYYRRYHVVNKHNIPPR
                     GEGFMVICNHQNGLNDALGILFSMNGRFPVFIARADIFKIKFVAKLLRFLKIMPAFRK
                     RDVGMAGLDNNEIIFAHSARIISEGDIITLFPEAGHEDCHHLGTFKKGFARIAFRAVE
                     LSDYQLKLKILPLGNHYSNYFRMQGKLMINIGEPFEFTELYELHKTDPNRAQYLLAQK
                     ARERVKKLMLDVEDLDYYDEYIMLCTIYRDTYIRHRPPRDGRPEKRTSIFCRIRYNNP
                     GYFPNELEADQHIVATLDELKAKDRPAFEQLMTKAGQYKKNLKQLKLRDWIFRRRFLA
                     GFWIRTIALLLFSPFWLFGYLNNALPFYAGNLITRRVKDRMLHSSFHLGLGTLVTFPL
                     WYLILFSTVWIISGTFWMAAAYLAVLPLSLL"
BASE COUNT          402 a          366 c          336 g          395 t
ORIGIN      
        1 aacggggagc ccagaatggg cacccacacc tggcccgaaa tgaattcggc cactatgact
       61 gtggtccagg acgatcaggt ggaggaattg cttgattgta tcaaaaaact ggataaacgt
      121 aacgaagaaa tcggtgtcag ggcttttgtc tggaatattg aacaaactgt ctaaaatcgg
      181 cagattctaa taaaactctt atctttggcc cggattcaaa aacaacgcta tgttagattt
      241 tagacgggtc aacaaacctg atttcaggta ttccttattg taccgctacg tacaatttgt
      301 tcacaatcac atctactaca gacgttacca tgttgtaaac aagcacaata tccctccccg
      361 gggcgaaggc tttatggtaa tatgcaacca ccagaacggc ctgaacgatg cgcttggaat
      421 tttgttttcg atgaacggac gctttcctgt ctttattgcc agggctgata ttttcaaaat
      481 aaagtttgtg gccaaattgc tgcgtttttt gaaaattatg cctgctttcc gcaaaagaga
      541 cgtcggaatg gcaggcctcg acaacaacga aatcattttt gcccactcgg ccaggattat
      601 cagcgaaggg gatattatta ccctcttccc tgaggcagga cacgaagatt gtcatcacct
      661 gggcacgttc aaaaaaggtt ttgcccgtat tgccttcagg gcagtggaat tgtcggatta
      721 tcaactaaaa ctcaaaatcc tgccgttggg caatcactat tcgaattatt tcaggatgca
      781 gggtaagctg atgatcaaca tcggagaacc ttttgagttt accgaacttt acgaactgca
      841 caaaaccgat cccaaccggg cgcaatacct cctggctcaa aaggcccgcg aaagggtaaa
      901 aaaattgatg ctcgatgtcg aagacctgga ttattacgat gaatacatca tgctctgcac
      961 catttaccgg gatacttaca tccggcatcg tccgccccgg gatggccgcc ccgaaaagcg
     1021 gacttcgata ttttgccgga tccgttacaa caatccgggc tacttcccca acgaactgga
     1081 agccgatcaa catatagtgg ccaccctgga cgaattaaaa gcaaaagacc ggccggcttt
     1141 tgaacaactt atgacaaaag cggggcaata caaaaagaac ctcaagcagc ttaagctaag
     1201 ggactggatt ttccgcagac gctttctggc aggcttctgg atccggacca ttgctttatt
     1261 gcttttttcg cccttctggc tgtttggcta cctcaacaac gcattgcctt tttacgccgg
     1321 caacctgatt acccgcaggg tcaaagacag gatgttgcat tcttcttttc atcttggcct
     1381 gggtaccctg gttactttcc cgctctggta tttgattctg ttctcaacag tttggataat
     1441 aagcggcact ttctggatgg cggcggccta cctggctgta cttcccttga gtctgctca
//
LOCUS       DAOSGL010000396         1491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_88796_length_1491_cov_4.199861, whole genome shotgun sequence.
ACCESSION   DAOSGL010000396 DAOSGL010000000
VERSION     DAOSGL010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88796_length_1491_cov_4.199861"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1206
                     /locus_tag="PKY68_09525"
     CDS             61..1206
                     /locus_tag="PKY68_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOH25305.1"
                     /translation="MRNLFFITGLFILLQACSDNTSFVVEGHVENAENKTLYFEFFGL
                     SNTSIIDSVVLDESGKFKFKAPLPEYPDFYRLRLDRQRLFLAVDSQVHINIKASAEAF
                     STGYRVSGSDYCVQMQELALLQSNTRRQYDSIQALFNNKEIEEKAYIDLVLNCIDEHK
                     KQAQGYIFSKPASPVAYFALFQRIHKLLIFDPFSEKDNRHFAAVATAWNVHYPHSERT
                     RQLVNLTMQGMSSIRKQRITPQDIPIIEIDALKYFEINLPNVFGQNIPLSSLEGKVIL
                     LDFTAFQANYSAARTIEMRELYNRYELYGFEIYQVSLDPDEHFWKTGALNLPWICVRD
                     KNLLNSAYLSMYNVSNLPSYFLIDRQGNLAGRDETIQNLDEAIQKLL"
     gene            1386..>1491
                     /locus_tag="PKY68_09530"
     CDS             1386..>1491
                     /locus_tag="PKY68_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOH25306.1"
                     /translation="MATNYMTENGLNKLKEELEHLETVERAYISKQIAE"
BASE COUNT          448 a          329 c          290 g          424 t
ORIGIN      
        1 ctccggctgt ttggctaaaa ttttttataa tttcgcccac actttcgtta atcgcagtaa
       61 atgagaaatt tattttttat aacgggcttg tttatactat tacaggcttg ttcagacaac
      121 acaagcttcg ttgtggaagg ccatgttgaa aatgcagaaa acaagacctt gtattttgaa
      181 ttttttggcc tgagcaacac ttccataatt gactccgtgg tattggacga atcgggcaaa
      241 ttcaaattca aagctcctct gcctgaatat cccgattttt atcgcttgcg tttagaccgg
      301 caacgccttt ttttggctgt tgactcacaa gtgcatataa acatcaaggc ctctgcggaa
      361 gctttttcga ccggctaccg ggtaagcggc tccgactatt gtgttcaaat gcaagaactc
      421 gctttattac aaagcaatac acggagacaa tacgactcca tccaagccct tttcaacaac
      481 aaagaaatag aagaaaaagc ttacatcgat ctggtgttga attgcattga cgaacataaa
      541 aaacaggctc aagggtatat tttttccaaa ccggcttcgc ccgtagctta ctttgctcta
      601 ttccagcgca tacataaatt actgattttc gacccctttt cagaaaaaga caatcgtcat
      661 tttgcagctg ttgctactgc ctggaatgtg cattaccccc attcggagag aaccaggcaa
      721 ctggtcaacc tgactatgca gggtatgagc agcatccgta agcaacgcat tacacctcaa
      781 gacattccta tcatcgaaat tgatgctctc aagtatttcg aaatcaacct gcccaatgtt
      841 tttggtcaaa atataccctt atccagcctc gaaggcaagg ttatattgtt ggacttcacc
      901 gcatttcagg caaactattc ggcagcccgc accatcgaaa tgagagaact gtacaataga
      961 tacgaactat acggttttga aatataccag gtgtcgctcg atccggacga acatttctgg
     1021 aaaaccggag ccttgaattt accctggatt tgcgtccgcg acaaaaacct gctcaactct
     1081 gcctatctgt cgatgtataa cgtcagcaac ttacccagct actttcttat cgacagacag
     1141 gggaatttgg caggcagaga cgaaactata cagaatttgg acgaggccat acaaaagctg
     1201 ctttaaatcc gaagatttag tcaaacaaaa aagcctcttt tgtttggcta tatgtatttt
     1261 taaatggggc caaaattttt tatatcactg agaattacat atctttgagc ctaattttga
     1321 aaggagttcc ggctgtttgc cgggattctt tttgtttaat ccctttgaaa gtttggattt
     1381 cgactatggc aacaaattac atgaccgaaa atggtctaaa caaattgaaa gaagaactgg
     1441 agcacctgga aacagtagaa cgggcttaca tctcaaaaca aatagcggaa g
//
LOCUS       DAOSGL010000397         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_90425_length_1473_cov_3.211566, whole genome shotgun sequence.
ACCESSION   DAOSGL010000397 DAOSGL010000000
VERSION     DAOSGL010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90425_length_1473_cov_3.211566"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PKY68_09535"
     CDS             complement(<1..631)
                     /locus_tag="PKY68_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOH25307.1"
                     /translation="MIHFFKTPTGNVLAVQADRNFSQEESSKLTWLFNQAQYLNSPQI
                     EGVFIGPRREMITPWSTNAVEIALNLGVKDIVRIEEFTPVASDKAEHDPMLQRVYKTL
                     DQKVFSIDKKPEPILYIQDIAAYNEQEGLALSPDEIEYLEKVSEKIGRDLTDSEVFGF
                     SQVNSEHCRHKIFNGTFTIDGETKQDSLFKLIRKTSETNPNKLVSAYKDN"
     gene            732..>1473
                     /locus_tag="PKY68_09540"
     CDS             732..>1473
                     /locus_tag="PKY68_09540"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOH25308.1"
                     /translation="MKKQTSLPLIQSLDALSFESESDGIRKLLYEQGSALKLDCLNWP
                     KDFPYAPKTLLWLGRSESGIYLHFVVEEIEKRINHHCDNAPVWEDSCVEFFVALPSEN
                     HYYNFEFNSLGCCLAARRKSREDYVLFGHEQLALIKRCPGGESVSPDAVVAGSAKQQV
                     FKPGIADKTAELFPEAGDKPAELIHGTSDETAEFFPERADKTAGLFPEGVGKPGLVWS
                     LTVYIPFRLLGVNCKSLPSVLRANFYKCC"
BASE COUNT          369 a          315 c          359 g          430 t
ORIGIN      
        1 cgttatcttt ataagcagaa accaatttat tgggattggt ttccgaagtt ttgcgaatca
       61 atttaaacag ggaatcctgt ttggtttctc cgtcaatggt aaatgtacca ttaaaaattt
      121 tatgcctgca atgttcggaa ttgacttgag aaaaaccgaa aacttcgctg tcggtaaggt
      181 cgcggccaat tttttcggat actttttcca ggtattctat ttcatcggga cttaaagcca
      241 ggccttcctg ttcgttgtag gcggcaatat cttgaatgta aagtatgggt tcgggttttt
      301 tgtcaatact gaacactttc tgatccagcg ttttgtaaac gcgttgcaac ataggatcgt
      361 gttcggcttt atcgctggct acgggagtaa attcttcgat gcggacgatg tctttaacac
      421 cgagattcag agctatttct acggcattgg tactccaggg ggtaatcatc tcgcgcctcg
      481 ggccgataaa aacgccttcg atttgtggag aattcaggta ttgggcctgg ttaaacaacc
      541 aagtcagttt actgctttcc tcttgggaaa aattgcggtc ggcttgcaca gcaagcacgt
      601 ttccggtggg ggttttaaag aaatgaatca tagggggatg gtttacagca ggactctcaa
      661 aatcctgctg caaaggtagc taaaaattca acatacgaac ttatatttgc aatcaagaat
      721 agccggtttt tatgaagaaa caaacctcct tacctttaat tcagtcttta gatgctctaa
      781 gctttgaatc ggaaagtgat ggcatacgta aattgctcta cgagcagggt tccgcactga
      841 agctggactg cctcaactgg ccgaaggatt ttccttatgc tcccaaaacc ctgctttggc
      901 tgggaaggag cgagtccggc atttatctgc attttgtggt cgaggaaata gaaaaaagaa
      961 taaatcacca ctgcgacaat gctcctgtct gggaagacag ctgtgttgaa ttttttgttg
     1021 ccctgccctc tgaaaaccat tattataact tcgaatttaa ctcgttgggt tgttgcctgg
     1081 cagccagacg gaaaagccgt gaagactacg tgcttttcgg tcatgaacaa ctggcattga
     1141 tcaaacgctg ccccgggggt gaatctgtca gccccgacgc tgtcgtcgcc ggatctgcca
     1201 aacaacaggt gtttaaaccg gggattgccg acaagaccgc tgagcttttt ccggaggctg
     1261 gcgacaagcc tgctgagctt atccacggca cttctgacga gacagccgaa ttttttccgg
     1321 agagagccga caagaccgcc ggacttttcc cggagggcgt tggcaagcct ggcctggttt
     1381 ggtctctgac ggtatatata ccctttaggt tattgggtgt caattgcaag agtttacctt
     1441 ctgttttgcg agcaaatttt tataagtgtt gtt
//
LOCUS       DAOSGL010000398         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_90783_length_1469_cov_3.752475, whole genome shotgun sequence.
ACCESSION   DAOSGL010000398 DAOSGL010000000
VERSION     DAOSGL010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90783_length_1469_cov_3.752475"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKY68_09545"
     CDS             complement(<1..255)
                     /locus_tag="PKY68_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOH25309.1"
                     /translation="MRIQDLIIKHNALKPEKGRILIAEPFLAGEPFERSVVLMCESGP
                     NGHLGFVLNKPIGFNLDEVVSTNERIPNIPIYLGGPVAAKR"
     gene            complement(272..>1469)
                     /locus_tag="PKY68_09550"
     CDS             complement(272..>1469)
                     /locus_tag="PKY68_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOH25310.1"
                     /translation="ATGQKYVRMEMGVPGLPPSKVGVEAEIAALQKGVASIYPMLDGH
                     PELKAEAARFVKAFIDVDVPAECCVPVTGSMQGSFTSFITCSQCNRSKDRLLFIDPGF
                     PVQKQQLVVLNTAYDSFDVYHYRGAALREKLESILSLGKTSALIYSNPNNPAWFCLKD
                     SELKIIGELAEKYDVIVIEDLAYFAMDFRQDLGRPFEPPFQPSVAKYTDNYILLISAS
                     KAFSYAGQRIGVAVLSEKLYKRPYADLKKQYGVCEFGNVFVNRVLYSLSSGTSHSAQY
                     AMAAMMKAASDGEYRFLDEVREYGRRAEKVKKLLLGNQFEIVYDNDLGEALADGFYFT
                     VRYPGMSGAELMKELLFYGISAISLETTGSDQQGLRICTSFIKDDQYEELESRLKKFR
                     ADHPLN"
BASE COUNT          312 a          363 c          405 g          389 t
ORIGIN      
        1 gcgtttggct gccaccgggc ctcccaggta aataggaatg ttggggattc tctcgttggt
       61 gctgaccact tcgtccagat tgaatcctat aggtttgttc agtacaaagc ccaagtgtcc
      121 gttcggccca ctttcgcaca tcagcaccac ggaacgttca aaaggttctc cggccagaaa
      181 gggttccgct atcaggatgc gtcctttttc gggcttcagt gcgttgtgtt tgatgataag
      241 gtcctggata cgcatgactt gaatcttttt cttagttcag cggatggtct gcccggaatt
      301 ttttaagccg ggattccaat tcttcgtatt gatcgtcttt gataaacgag gtacaaatcc
      361 tgaggccttg ctggtcactg ccggtggttt cgagcgagat ggcactgatg ccgtaaaaaa
      421 gcaattcttt catcaactct gcaccgctca tgcccgggta gcgcaccgta aaataaaacc
      481 cgtcggccag ggcttcgccc aggtcgttgt cgtagacaat ttcgaattga ttcccgagca
      541 ggagtttttt tactttttcg gcgcgtcggc cgtattccct cacttcgtcc aagaagcgat
      601 attcaccgtc ggaagccgcc ttcatcatgg cagccatagc gtattgggcc gaatgactgg
      661 tgcccgacga cagcgaatag agtacccggt tgacgaaaac gttgccgaat tcgcacacgc
      721 cgtattgctt tttcaggtct gcgtaagggc gcttatacag tttttcggag aggacggcta
      781 cgcctatgcg ttgtccggcg tagctgaagg ctttggaagc ggagatcagt aatatgtaat
      841 tgtcggtgta ttttgctacg ctgggctgga agggcggttc aaaaggtcgc cccaaatcct
      901 ggcggaagtc catggcaaaa taggccaggt cttcgataac gatgacatcg tatttttcgg
      961 ccaattcccc aattattttc aattcgctgt ctttcaggca gaaccaggcc gggttattgg
     1021 ggttggagta gatcagggcc gaggttttac ccaggctcag gatgctttcg agtttttctc
     1081 tcagggctgc cccccggtag tggtacacat cgaatgaatc gtaggcggtg ttgagcacca
     1141 ccagttgttg cttttgtacc ggaaagccgg ggtcgataaa gagcaggcgg tctttgctgc
     1201 gattgcactg actgcaggtg ataaaggagg taaagctgcc ctgcatggag ccggtgacgg
     1261 gcacacagca ttcggcgggc acatccacat ctataaatgc cttaacaaaa cgggcggctt
     1321 ccgccttgag ctccggatgt ccgtcgagca tggggtagat ggaagctacg cctttttgca
     1381 aggctgctat ttcggcttcg actcctactt tggagggggg aaggccgggc acgcccattt
     1441 ccatacgcac atacttttgt ccagttgct
//
LOCUS       DAOSGL010000399         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_91217_length_1464_cov_4.981547, whole genome shotgun sequence.
ACCESSION   DAOSGL010000399 DAOSGL010000000
VERSION     DAOSGL010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91217_length_1464_cov_4.981547"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..717)
                     /locus_tag="PKY68_09555"
     CDS             complement(22..717)
                     /locus_tag="PKY68_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOH25311.1"
                     /translation="MKKLVVLTGAGMSAESGIKTFRGSGGLWENYPIETVATPEGWKA
                     NPELVTQFYNDRRKQLLEVEPNAGHYELAAMEKSFDVQIITQNVDNLHERAGSSNVLH
                     LHGELTKVRGEVHSEYIVELKPEEYEVKIGDCTPYGDQLRPHIVWFHEMVTMIDPAMD
                     IVRQADYFAVIGSSLVVYPAAGLLHLVPRRSPSYVIDPEELSLCRFPDIVHIKLPASK
                     GVKKLHSLLLEQK"
     gene            876..>1464
                     /locus_tag="PKY68_09560"
     CDS             876..>1464
                     /locus_tag="PKY68_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOH25312.1"
                     /translation="MERISSLQNPKIKQLLRLQAKARERREQALVLVEGAREIQLALS
                     KGYRPQQLFICPELYARSDYPGLVGEIADEIKTELSLPVFEKLAYREGSDGLLATLRP
                     KSHLLEELVLSAHPFIVVLEAVEKPGNLGAILRTADAAAVDAVIVCDPLCDLYNPNVV
                     RSSVGCLFTVQTAVCSSQQAFDWMRRHKINILAAEL"
BASE COUNT          364 a          363 c          349 g          388 t
ORIGIN      
        1 gccaaacagc cggagccaac gttatttttg ctccaaaagc aagctgtgca gcttttttac
       61 acctttagaa gccggcaact tgatgtgaac aatatccggg aaccggcata aagaaagttc
      121 ttcgggatct attacgtacg aagggcttcg cctggggact aggtgcagca agccggctgc
      181 aggataaacc accaatgagc ttcctataac ggcaaaatag tctgcttgtc tgactatatc
      241 catagccgga tcaatcatag tcaccatttc gtgaaaccaa acaatgtgcg gacgcagctg
      301 atctccgtag ggtgtacaat ctcctatttt gacttcgtat tcctccggtt ttagttccac
      361 tatgtactcc gaatgtactt ctcctctcac tttggtcaat tcaccgtgta aatgcaagac
      421 attactgctg ccggcgcgtt cgtgcaagtt gtcaacattc tgagtaatta tttgcacgtc
      481 gaaagatttt tccatagcag ccaattcata gtggccggca ttgggctcca cctcgaggag
      541 ttgcttacgt cgatcgttgt aaaattgggt aaccagttcc ggattggctt tccagccttc
      601 gggagttgca accgtttcta tcgggtaatt ttcccataaa ccgccggatc cgcgaaaggt
      661 tttaatacca ctttcggcgc tcatgcctgc gccggtcaat actaccagtt ttttcatagt
      721 tctgtgtgta aaaatcgggc atggacgctt aatgtcaggc aatggccgga gaagctctgc
      781 cggcacccgc tgtttgcaca gcccgtaaaa caaaccatta caaatgtaac aaatatcgcg
      841 tatatttagg ccctaattct ccctaactta catacatgga acgaataagc agtctgcaaa
      901 atcccaagat aaagcaattg ttgcgtttgc aggccaaagc cagggagcgc agagaacagg
      961 ctctggtatt ggtcgaagga gctcgggaga tccaacttgc cctgagcaag ggatatcgtc
     1021 cccaacagct ttttatttgc cccgaacttt atgctcgtag cgattatccc ggactggtgg
     1081 gggaaatagc cgacgagatc aaaaccgaac tctccttgcc cgtcttcgaa aagttggcct
     1141 acagggaagg atccgacggt ttgctggcta cgctcaggcc taagtctcat ctgctcgaag
     1201 aactggtttt gtcggcacat ccttttatcg tagtgctgga agccgtcgaa aaacccggaa
     1261 atctgggagc tattttgcgt acggccgatg ctgcagccgt ggacgctgtt atcgtttgcg
     1321 atcctttgtg cgatttatat aaccccaatg tggtacgatc cagcgtaggc tgcctgttta
     1381 ccgtacagac ggccgtttgc agttcgcaac aggctttcga ttggatgagg cggcataaaa
     1441 tcaatatttt ggccgcagaa ttgc
//
LOCUS       DAOSGL010000400         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_92488_length_1451_cov_4.371777, whole genome shotgun sequence.
ACCESSION   DAOSGL010000400 DAOSGL010000000
VERSION     DAOSGL010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92488_length_1451_cov_4.371777"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKY68_09565"
     CDS             complement(<1..718)
                     /locus_tag="PKY68_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOH25313.1"
                     /translation="MDTFLIRLLQLITSLSILIVVHEFGHFLFAKLYKIRVERFYLFF
                     HPGFSLMRFKKIEGKYEFSFLSKNPPEHWKDYPETTMWGLGWLPLGGYCSIAGMVDET
                     KSSKDLASEPQPWEFRTRKASQRLMVMVGGVLFNFILALVLYAAVLGIWGNSFLPLQN
                     ATLGMEFSEPALKAGFQNGDILLEADGIALEQFNDDSFRKIVEASEVKVLRKGEEAKV
                     AIPEDFMQQLLSARQGFAAFR"
     gene            complement(741..>1451)
                     /locus_tag="PKY68_09570"
     CDS             complement(741..>1451)
                     /locus_tag="PKY68_09570"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOH25314.1"
                     /translation="DSEHSAIFQCLEFANPPEKIILTASGGPFRKFSMEELKTVSREQ
                     ALKHPNWAMGDKITIDSATMMNKGFEVIEAKWLFGLLPEQIEVVVHPQSIVHSMVQFR
                     DGSVKAQLGLPDMKLPILYALSYPLRRETRYPRLDITEIGCLEFEKPDLNKFRNLALA
                     YTAMETGGNMPCILNAANEVAVDAFLHGRIAFLQMSDLLEFTMEKAEFFKCPDYEAYT
                     ISDQRARLIAQAYLDKLS"
BASE COUNT          412 a          351 c          317 g          371 t
ORIGIN      
        1 aacggaaagc ggcaaagcct tgtctggccg acagtaattg ctgcataaaa tcttcaggaa
       61 tagcgacttt agcttcctct cccttgcgca agacctttac ttcggaagct tctactatct
      121 tgcgaaagct gtcgtcatta aactgttcaa gggctatacc gtctgcctcg agcaaaatat
      181 ctccgttctg aaagccggct ttcagcgccg gttccgagaa ttccattccc aatgtggcgt
      241 tttgcagagg caggaaagaa ttcccccaaa tgcccaaaac agctgcgtat aacaccagag
      301 cgagaataaa attgaacaac actcctccca ccattaccat cagtctttga ctggccttgc
      361 gggtgcgaaa ctcccagggt tgaggctccg aagcaagatc ctttgaactt tttgtctcat
      421 ctaccatgcc cgctatggag cagtagccgc ccaaaggcaa ccagccaaga ccccacatag
      481 tggtttcggg gtagtctttc cagtgttcgg ggggattttt agacaaaaag ctgaattcgt
      541 atttgccctc gattttctta aaacgcatca gagaaaaacc cggatggaaa aataaataaa
      601 agcgctccac gcgtattttg taaagcttgg caaacagaaa atggccaaac tcatgaacga
      661 cgattaatat ggacagactg gtaataagtt gaagtaatct gatcaaaaaa gtatccatag
      721 ttcaggtgta aatagattga ttaggacaat ttgtccaaat aggcctgtgc tattaggcga
      781 gcacgttgat cactgatggt ataggcttcg taatcggggc atttaaaaaa ctcagctttt
      841 tccatcgtaa attcaagcaa atcggacatt tgcaaaaagg ctatacgacc gtgcagaaaa
      901 gcatcgacgg ccacttcgtt ggcggcgttg agaatgcagg gcatattacc acccgtctcc
      961 atagccgtat aggccagggc taaattcctg aatttattca ggtcgggttt ttcgaactcc
     1021 aaacagccta tctctgttat atccaatctg gggtaacgag tctcccgacg taatggataa
     1081 ctcagagcgt acaaaatggg aagcttcata tcgggcaaac ccaactgagc ttttacagag
     1141 ccgtctctga actgtaccat agaatgtacg atcgattgag gatgcaccac tacttcaatt
     1201 tgctcgggca gcaagccaaa cagccattta gcttcaatca cctcaaaacc tttgttcatc
     1261 atggtggccg agtcgatggt aatcttgtcg cccatagccc aattaggatg tttcagagct
     1321 tgctccctgc tcaccgtctt gagttcttcc atgctgaatt ttctaaacgg gccgcccgaa
     1381 gccgttaaaa tgattttctc cggtggattg gcaaattcca ggcattgaaa aatagccgaa
     1441 tgctccgaat c
//
LOCUS       DAOSGL010000401         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_94110_length_1435_cov_3.762319, whole genome shotgun sequence.
ACCESSION   DAOSGL010000401 DAOSGL010000000
VERSION     DAOSGL010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94110_length_1435_cov_3.762319"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKY68_09575"
     CDS             complement(<1..282)
                     /locus_tag="PKY68_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25315.1"
                     /translation="MDGSYFNTSKGEQTALILLLSGIIALKSLCFDYWHLQHPRVQKR
                     LAMDSVRYELQTYALQYRADGRYGISGSSESFPEKLPETVLSLREFDPNT"
     gene            417..824
                     /locus_tag="PKY68_09580"
     CDS             417..824
                     /locus_tag="PKY68_09580"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOH25316.1"
                     /translation="MNKPFLKRFPRFFFSSLWALGIFGLFVFDFSETTKDLPLIPYAD
                     KIVHALLFFVQTGLLLWELNFRNLTSTSIPVGRLFIWSVSLPFLYGVLIELIQHFLPY
                     RGAELMDLLFDLMGSLAAFSCLVLILIWRENKR"
     gene            904..>1435
                     /locus_tag="PKY68_09585"
     CDS             904..>1435
                     /locus_tag="PKY68_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH25317.1"
                     /translation="MKSLLVFLLLTAAGFNAYSSAKIWVSPEGSDSNPGTEQQAMASL
                     QGALRKAREMRRLQDPAIEKGIEIILKDGYYALPETLLIRPEDSGSRNSPTTIKSEHY
                     GKAVIGGGLKIEDWEPLKKRVKGMNKSIGSKVWVAKVPTCGGRRIDFRQFYVNGRKAG
                     AAAHSDTSRMQRILAVD"
BASE COUNT          368 a          337 c          309 g          421 t
ORIGIN      
        1 cgtgttagga tcgaattccc gcaaggacag tactgtttcc ggcagctttt cagggaagct
       61 ctctgacgat ccggaaattc cataacggcc gtcagcacga tattgtaagg cataagtctg
      121 caattcataa cgcacagaat ccatggccaa acgtttttga acacggggat gttgcaaatg
      181 ccagtaatcg aagcaaagag atttcagggc aattattccc gacaataaga gaatcaatgc
      241 cgtctgttca cctttgctcg tattaaaata ggaaccgtcc atgagtgtat tttacattgc
      301 aaatatactt ttttaatatt tacataatat acgagtccgc catttataaa gtgaattttc
      361 tacacaataa aagttttaaa tagtgtatat ttgtaccttg ttcgtcaact aagattatga
      421 ataaaccctt cctgaagcgt ttcccgcgat ttttcttttc atcgctgtgg gctctcggta
      481 tttttggcct tttcgttttc gacttttccg aaaccaccaa agacttaccc ctgattccct
      541 atgccgacaa aatcgtgcat gccctgttgt tttttgttca gacaggcctt ttgctgtggg
      601 aactcaattt tcgaaatctt acctctacct ccattcccgt cggccgttta tttatttggt
      661 cggtatcgct tcctttttta tacggtgttt tgatagagct tattcaacat tttctaccct
      721 accggggagc tgaacttatg gatttgcttt ttgatctgat gggttcgctg gcggcctttt
      781 cctgcctggt tttgatactt atttggcgtg aaaacaagcg ttaaggagtt tttttgcttt
      841 ttggcaaagc aaatcaaagg cttttcctat ttttgttttt tcattgcttc aacccaccat
      901 actatgaaat cattgctcgt ttttctcctc ttgactgcag ccggattcaa tgcctactct
      961 tcggctaaaa tctgggtgtc gccggagggc tccgactcca accccggcac ggaacagcaa
     1021 gccatggcca gccttcaggg ggctttgcgc aaggccagag aaatgagaag gctacaagat
     1081 ccggccatcg aaaaaggaat agaaatcatt ttgaaagacg gttactacgc tttgcccgaa
     1141 accttattga ttcgtcccga agactccggc agccggaaca gccctaccac cataaaatca
     1201 gaacattacg gcaaggccgt tatcggcgga ggtctcaaaa ttgaagactg ggaacccttg
     1261 aagaagcggg taaaaggaat gaacaagagc atcggttcaa aagtatgggt ggccaaagtc
     1321 cccacctgcg gaggacgcag gatcgatttc cggcagtttt acgtaaacgg tagaaaagcc
     1381 ggggctgccg cccattccga cacctcccgt atgcaacgca ttctggccgt agatt
//
LOCUS       DAOSGL010000402         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_95283_length_1424_cov_3.089847, whole genome shotgun sequence.
ACCESSION   DAOSGL010000402 DAOSGL010000000
VERSION     DAOSGL010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95283_length_1424_cov_3.089847"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PKY68_09590"
     CDS             <1..1181
                     /locus_tag="PKY68_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25318.1"
                     /translation="GEISPKLLRKFGITEGNRQTFLLGMFMSQLVNPNRWTVYPDFAN
                     SCGPEGEKLEVWAYREWHGEAHVGETPPQLIAETREHARKAVEAIDKVTRVRKNKEEF
                     ARLKNDIYAYKAFTDCFTDKLEASMLVLRYSYSNEISDLEQAYSLMESSLDHYRKLEE
                     LTRDSYLYANSMQTGMRRIPVTGNNGANKTWTELLPQFEQELARFDANIKKLKSGNQT
                     PENLKVLSPAEVKLISPARAEFYQLQEGASVYSDRPFKIKALAPELSQLKALRMSNRQ
                     QLNENIRLRFNSREAVDLWVAYFKPFRQGDYMPAPRLETDASANQYGQADIKIANAME
                     IENLPAVNVHQYRFEAGEHNLNLGKGLCLVLGFSQPEAAFSTRDAGFMEKTAIDWLFY
                     "
     gene            1232..>1424
                     /locus_tag="PKY68_09595"
     CDS             1232..>1424
                     /locus_tag="PKY68_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25319.1"
                     /translation="MNAKNLITFSLIMLLCGSVAEISAFKKQSSTPGFDPLQTAEIYL
                     SGHGPDDAVEWDFMCTGGRN"
BASE COUNT          405 a          387 c          326 g          306 t
ORIGIN      
        1 gtggagaaat atcgcccaaa ttgctgcgca aattcggtat caccgagggt aaccgacaaa
       61 ccttcctgct gggaatgttt atgagtcaat tggtcaaccc caaccgctgg acggtctatc
      121 ccgattttgc caattcctgc ggccccgagg gtgagaaact cgaagtttgg gcctaccgcg
      181 aatggcatgg agaagcacat gtgggcgaaa ctcctcccca actgatagca gaaacacggg
      241 aacatgcccg gaaagcggtg gaagccatcg acaaagtgac tcgtgtgcgc aaaaacaaag
      301 aagaatttgc acgtctcaaa aacgatattt atgcctacaa ggcttttaca gactgcttta
      361 ccgataaact ggaagcctcc atgctcgttt tgaggtattc ctattccaac gaaatttccg
      421 atctggaaca agcctacagc ctgatggaaa gcagtttgga tcactaccgc aagctcgaag
      481 agctcacccg cgacagctat ctctacgcca acagcatgca aaccggaatg cgccgcattc
      541 ccgtcaccgg caacaacgga gccaacaaaa cctggacgga attattgcct cagttcgaac
      601 aagaactggc gcgtttcgac gccaatatta aaaaactcaa atcgggcaat cagaccccgg
      661 aaaacctgaa agtactgagt ccggccgaag tcaaacttat ttctcctgcc cgggcggaat
      721 tttatcagct tcaggaaggg gcaagcgtgt acagcgaccg acctttcaaa ataaaagccc
      781 tggctccgga actcagtcaa ctcaaggctc tgcgtatgag caaccgccaa caactcaatg
      841 aaaacatccg cctgcgcttc aacagccggg aagccgttga cctttgggtt gcttatttca
      901 agcctttcag gcaaggagac tatatgcccg ctccccgtct ggagaccgat gccagcgcca
      961 atcaatacgg tcaggcggac atcaaaattg ccaacgccat ggaaatagaa aatctgccgg
     1021 cggtgaatgt gcatcaatac cgtttcgaag ccggcgaaca caatctgaac ctgggtaaag
     1081 gtctttgcct ggtgctgggt ttctcccagc ccgaagcggc atttagcacg agagacgccg
     1141 gttttatgga aaagacagcc atcgactggt tgttttattg atactatccc tctaaagata
     1201 ccatcccctt aaacactata caaaacactt tatgaacgct aaaaacctta taacattctc
     1261 cttgataatg ctgctttgcg gcagtgtcgc cgaaatttcc gcctttaaaa aacaaagttc
     1321 gacccccggc ttcgatccct tacaaacagc cgaaatctat ctgtccggtc atggcccgga
     1381 cgatgccgtt gaatgggact ttatgtgcac cggaggccga aaca
//
LOCUS       DAOSGL010000403         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_96931_length_1408_cov_3.074649, whole genome shotgun sequence.
ACCESSION   DAOSGL010000403 DAOSGL010000000
VERSION     DAOSGL010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96931_length_1408_cov_3.074649"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PKY68_09600"
     CDS             <1..765
                     /locus_tag="PKY68_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOH25320.1"
                     /translation="RTNRKAFCYLFAPPLELLTPDAKRRLQAIENFSELGSGFNIAMQ
                     DLDIRGAGNLLGAEQSGFIADLGFEAYQRILNEAMQELRDEEFPLSSEQEEANRADCC
                     YVSDSLLESDLSLYFAENYVPGSSERMLLYREMDQLEKEEDIRAFEKRMIDRFGPIPS
                     EGQDLIDVVRLRISAGQLGMEKIILKNNQMICHFVSNPKSSFYQSSCFDRILNYVQQH
                     YKKTRLREQQGKRSLLIEQVGSVKEAHTILLSMLQS"
     gene            834..>1408
                     /locus_tag="PKY68_09605"
     CDS             834..>1408
                     /locus_tag="PKY68_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOH25321.1"
                     /translation="MIQISFPDGSVRSYEKGTTAYQIAESISPRLAKESLAASVNGIT
                     RDLNLPINEDAQVQLLKWDEPEAKHAFWHTSSHLLAAALQELYPGIQFGIGPAIENGF
                     YYDVDPGDGVIIKESELPAIEAKMTDLLANKFVLQRREISKNEALKYFKQRGEHYKVE
                     LIRDLEDGTISTYTLGNFTDLCRGPHLTDTAP"
BASE COUNT          400 a          353 c          313 g          342 t
ORIGIN      
        1 cgtaccaacc gtaaagcttt ctgttatctc tttgcccctc ccctggaatt gcttaccccc
       61 gacgccaaac gccgtctgca agccatagag aacttcagcg aactgggtag cggcttcaat
      121 atcgccatgc aagacctcga catcagaggt gccgggaact tgctgggagc cgaacaaagc
      181 ggctttattg ccgatctggg ttttgaggcc tatcagcgca ttctgaatga ggctatgcag
      241 gaactcaggg acgaagaatt ccctctcagt tcagaacagg aagaagccaa ccgggcagat
      301 tgctgttatg tcagcgatag cttgctcgaa tcggacctct ccttgtattt tgccgaaaat
      361 tacgtaccgg gctcgtcgga acgcatgttg ctttatcgcg aaatggatca gctcgaaaaa
      421 gaagaagaca tccgggcttt cgaaaaacga atgatcgacc gtttcgggcc tattcccagt
      481 gaaggccagg atttaatcga cgtagtgcgt ttgcgtatct cggccgggca actgggcatg
      541 gaaaaaatta ttcttaaaaa caatcagatg atttgtcatt tcgtatccaa cccgaaatcc
      601 tctttttatc agtccagttg ttttgaccgt attctgaatt atgttcagca gcattacaaa
      661 aagaccaggt tgcgcgaaca acaaggtaaa agatccctgc tcattgagca agtaggcagc
      721 gtaaaggaag cccataccat acttctttcg atgcttcagt cataaaaaga agcattaaag
      781 caacttgagg aatttatttt ttgtactttt gtgcgttttt taatacacag attatgattc
      841 aaatcagctt tccggatgga tccgttcgct cgtatgaaaa aggaaccacc gcttatcaaa
      901 tagcagaaag catcagcccc aggctggcca aagaaagcct ggccgcctcc gtcaacggta
      961 ttacccgcga tctgaacctt cccatcaacg aagacgctca ggtacaatta ttaaaatggg
     1021 atgaaccaga agccaagcac gctttctggc acacttcttc tcacctgctg gctgccgccc
     1081 tgcaagaatt gtatccgggc atccagttcg gtataggacc tgccatcgaa aacggttttt
     1141 attacgatgt cgatcccggc gacggagtca ttattaaaga aagcgaattg ccggccatcg
     1201 aagccaaaat gacagactta ctagccaaca agttcgtgct gcaacgccgg gagatttcca
     1261 aaaacgaagc tctgaaatat ttcaaacaaa gaggcgaaca ttataaagtt gaacttatcc
     1321 gggatttaga agacggcacc atcagcacct acaccttagg caatttcacc gatttgtgcc
     1381 gtggccccca cttaacagac acagcccc
//
LOCUS       DAOSGL010000404         1393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_98511_length_1393_cov_4.849776, whole genome shotgun sequence.
ACCESSION   DAOSGL010000404 DAOSGL010000000
VERSION     DAOSGL010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98511_length_1393_cov_4.849776"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKY68_09610"
     CDS             complement(<1..199)
                     /locus_tag="PKY68_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25322.1"
                     /translation="MSRCRPCEYQTISTLSLLDSLRVGNSIYRGVMHFCCLDLAEEWG
                     NHTIKEIYLAKNYGLILYKLQN"
     gene            complement(177..>1393)
                     /locus_tag="PKY68_09615"
     CDS             complement(177..>1393)
                     /locus_tag="PKY68_09615"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOH25323.1"
                     /translation="INRMDYDKQGRLHVSWCWRDDFGGQTNHDLCYAYSDDHGRSWKD
                     TYGNRVATSEAINYTDSRTPGSCLRQGIATLKIADIPHNKGYINQETQSCDSKGRVHI
                     VNSYMDSGTESNWSSSRSKAVLHHRFRDTDGTWHHNYIYNKGQKVNSYCRVQIILDAF
                     DNAYVVANGAEIYAATSAAAYSDWELVSDLDAGRFCSEPQIDRNAIDEGLLSFVYLAR
                     NNELSIIDYLLDNPKPATGSGLKASFYSDSRFSNCIDSALSLPGSPLNTNVKSLRWSG
                     SLETRYGEAYSLYLNTTAASRLYINGKILLNTSHTGEPVEYAVELPPFNSHRHNIVIE
                     SLAGRQEVLELKWSSKRTEKQLIPLESLYPEPMDFWDDSREQLISLVYEQKLRCRAAV
                     GFPVVDEQVPAL"
BASE COUNT          311 a          333 c          364 g          385 t
ORIGIN      
        1 cattttggag tttgtatagt ataagtccat aattcttagc cagataaatt tcttttatcg
       61 tgtgatttcc ccattcctct gccagatcaa ggcagcaaaa atgcattact cccctataaa
      121 tgctgtttcc tacccggagg ctgtccagca ggctgagtgt agaaatggtt tggtattcac
      181 agggccggca cctgctcatc aacaacagga aaaccaacag cagcacgaca acgaagtttt
      241 tgttcatata ctaaagagat tagttgttcc cggctgtcgt cccaaaaatc catcggttcg
      301 ggataaagcg actcgagcgg aatcagttgt ttttcagtgc gtttgctcga ccacttgagt
      361 tccagtacct cttgccgtcc ggccaggctt tcgattacta tattgtgccg gtgactgttg
      421 aaaggaggca gttctaccgc atattctacc ggctccccgg tatgggaggt gttcagtaaa
      481 atcttgccat tgatatagag tctgctggct gcggtggtgt tcagatacag agagtaggct
      541 tctccgtacc gggtttcgag gctgcccgac cagcgcaggc tttttacgtt ggtattaagg
      601 ggactaccgg ggagactcaa ggcggaatcg atgcagtttg aaaaacggct gtccgaataa
      661 aaagatgcct taagcccgct gcctgtagcc ggttttggat tgtccaacaa atagtctata
      721 atgctaagct cgttgtttcg ggccagatag acaaaagaca acagaccttc gtcgatggcg
      781 ttccgatcga tttgcggctc tgagcaaaaa cgtcccgcat ccaggtcgga aaccaactcc
      841 caatcactgt aagcggctgc cgaggtggca gcgtagattt ctgctccgtt ggcaacgaca
      901 taggcattgt cgaaagcatc gaggatgatt tgaacccgac aataggaatt tactttttga
      961 cccttgttgt agatgtaatt gtgatgccag gtgccgtcgg tatcgcggaa gcggtggtgc
     1021 aacacggctt tgctcctgga gctgctccag ttggactcgg tgcccgagtc catataagag
     1081 ttgactatat gtaccctccc tttgctgtcg caggattgcg tttcctgatt gatgtagcct
     1141 ttgttgtggg gaatatcggc tatttttagt gttgcgatgc cctgcctcag gcaactgccc
     1201 ggagtccggc tgtcggtata attgatggct tcgctcgtag ccacccggtt tccgtaggtg
     1261 tctttccaac tgcggccgtg gtcgtcgctg taagcatagc aaaggtcgtg gttggtttgt
     1321 cccccaaaat cgtctctcca gcaccaggat acatgcaggc ggccttgttt gtcgtaatcc
     1381 atgcggttga tgt
//
LOCUS       DAOSGL010000405         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_99430_length_1385_cov_4.045113, whole genome shotgun sequence.
ACCESSION   DAOSGL010000405 DAOSGL010000000
VERSION     DAOSGL010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99430_length_1385_cov_4.045113"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKY68_09620"
     CDS             <1..913
                     /locus_tag="PKY68_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25324.1"
                     /translation="YARISSAAIPDSSESDPFKQMVYHRENLVWLGGRLASTGDSALR
                     FNVDAKICMLGSDYAGNFEIKGDVSSRFRLWGRPVYLAADGFVVSRMPDYFLRRYYSN
                     HFDWNNAFRNEYKTRLSGSLSIPALGFDLSAGVENISNYVYFDRKALPAQYDGQIQVL
                     FAAWKQHLGYGIINLDNEAVFQLSGKEEILPLPRLSLYTNLYAKTKLFDVLTLQAGVD
                     CRYHTAYYVPKYMPATGQFYLQDELKIGNYPFMNAYANFHLKRARFFVMYTHGSRWFA
                     DPNYFSLPHYPLNPAGIKAGLSWNFYD"
     gene            917..>1385
                     /locus_tag="PKY68_09625"
     CDS             917..>1385
                     /locus_tag="PKY68_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOH25325.1"
                     /translation="MNTLIEQEIQQLRRELHQADHEYYQLSAPRMKDFDYDVKMRRLQ
                     ELEQAHPELITPDSPTQRVGSDLNRAFTQEEHRYPMLSLSNTYSEEEVRDFYDRTLKS
                     LDEEPELVCELKFDGTSISLTYEEGILIKALTRGDGQKGDNVIQNIRTIRSIPL"
BASE COUNT          339 a          357 c          322 g          367 t
ORIGIN      
        1 atacgcccgt atttcgtcgg ccgccatacc cgacagcagc gaatcggatc ctttcaaaca
       61 aatggtctat cacagggaaa acctggtttg gctgggcggc cgcctcgcat caaccggtga
      121 ttctgcctta cgttttaatg tggatgccaa aatctgcatg ttggggagcg actacgccgg
      181 caattttgaa atcaaaggcg atgtctccag ccgtttcaga ctctggggca ggccggttta
      241 tcttgctgcc gacggatttg ttgtgagccg tatgcccgat tattttttgc gcaggtatta
      301 ttccaatcat ttcgattgga acaatgcttt tcgcaacgaa tacaaaaccc ggctttcggg
      361 cagtttgtcc ataccggctt taggttttga tctgtcggcc ggcgtggaaa acatcagcaa
      421 ttacgtttac ttcgaccgaa aggctttgcc ggctcaatac gacggtcaga tacaggtttt
      481 gtttgcggcc tggaaacaac atctggggta cggcatcatc aatctcgaca acgaagccgt
      541 ttttcaactc tccggtaaag aagagattct gcctttgccc cgcctgtctt tgtacaccaa
      601 tctttacgct aaaaccaagt tgtttgatgt cctgactttg caggccggtg ttgattgccg
      661 ctatcacaca gcttattacg ttcccaagta catgcctgcc accggacagt tttatctaca
      721 ggacgagttg aaaataggga attatccttt tatgaatgcc tacgccaatt ttcacctgaa
      781 acgggcacgc ttttttgtga tgtatactca tggcagtcgc tggtttgccg atccgaatta
      841 tttttccctg ccgcattatc cgctcaatcc cgccggtatc aaggccggct tgtcctggaa
      901 tttttacgat tagcccatga atactcttat agaacaagaa atacagcaat tgcgccggga
      961 gcttcatcag gccgatcacg aatattatca actgtcggct cccaggatga aggattttga
     1021 ttacgacgtc aaaatgcgcc gtttgcaaga gctcgaacaa gctcatccgg agctgatcac
     1081 ccccgattcg cccactcagc gcgtgggcag cgatttgaac cgggctttta cgcaggaaga
     1141 gcatcgttat cccatgctct cgttgagcaa tacctattcc gaagaagaag tccgtgattt
     1201 ttacgacaga accctcaaaa gcctcgacga agagcccgaa ttggtctgtg aactgaaatt
     1261 tgacggcact tccatatcgc tgacttacga ggagggtatt ttgatcaaag ccctgaccag
     1321 gggagacggc caaaaaggcg acaatgtgat ccaaaacatt cgcaccatcc gcagcattcc
     1381 tctca
//
LOCUS       DAOSGL010000406         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_101437_length_1367_cov_4.663110, whole genome shotgun
            sequence.
ACCESSION   DAOSGL010000406 DAOSGL010000000
VERSION     DAOSGL010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101437_length_1367_cov_4.663110"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PKY68_09630"
     CDS             complement(<1..1068)
                     /locus_tag="PKY68_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25326.1"
                     /translation="MRPKLFIIAMLALTLSSCTRDILPEPPKQEGNIVTISATIPLET
                     RVAYTNTYTPGSGGPLAWQANDQIILAGYNGDTYIGSSLFTFVTGSTDQFSGTLMDGA
                     TPTTVYKAYYNSAGAITLDDDGKIYLPADFWQQTQNGTNSTAHIGKKILLSDTEANLI
                     SQRFDLTANSSIIRVNLENLPTAIHSVKKVIWTVETTTGGTRSAILNVSGVIKPTIPG
                     QPFYMNAYLAFDPTVMNIAAGGKVKVTVIGTNGSIEWSKTVAAGMNYVAGNRYTGTVS
                     SWATAQAAFRFTIDITDPTQPYEIWQHSAPSTCPANLTINWGDGASQTINKYASLPDD
                     AIASHTYAGAGTYTITIYSDQP"
     gene            1253..>1367
                     /locus_tag="PKY68_09635"
     CDS             1253..>1367
                     /locus_tag="PKY68_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOH25327.1"
                     /translation="MNYIKTAIEGPVIIEPKVFYDHRGYFFESFSLREFEAN"
BASE COUNT          328 a          277 c          369 g          393 t
ORIGIN      
        1 gggctggtcg gagtaaatgg tgatggtata agtgcctgcg ccggcgtaag tatgcgaagc
       61 gatggcatcg tccggaagcg aggcgtattt attgatggtt tgggaggccc catctcccca
      121 gttaatcgtc agattggcag ggcaggtgga gggagcggaa tgttgccata tctcgtatgg
      181 ctgagttggg tcagttatat caattgtaaa acggaatgca gcctgagcag ttgcccaact
      241 gcttacggta ccggtgtagc gatttcccgc tacgtagttc atacctgctg caacagtctt
      301 actccactcg atagacccat tagtaccaat caccgttact ttcacctttc cgcccgctgc
      361 aatattcatc acggtaggat caaaagcgag gtacgcgttc atgtagaatg gctgtccggg
      421 gattgtaggt ttgatgactc cgcttacatt gagaattgcc gatcgtgttc ctcccgttgt
      481 ggtctcaacc gtccaaatca ctttctttac actatgaata gctgtcggca agttttcgag
      541 gttaactctg attatgctgc tgttggcggt caagtcgaag cgctgactta tgagattagc
      601 ctccgtgtcg ctcaagagaa tttttttgcc gatatgtgcg gttgagttgg ttccgttttg
      661 tgtctgttgc caaaagtcag cgggaagata gattttgccg tcatcatcca gtgtaatggc
      721 acccgcggag ttgtaatagg ccttataaac tgtggtgggg gtagcgccat ccatcaacgt
      781 tccggaaaat tggtcagtac ttccggtgac gaaagtaaag agagagcttc caatataagt
      841 atcaccgttg tatcctgcca gaatgatctg gtcgttggcc tgccaggcga gtgggccacc
      901 gctaccggga gtgtaggtgt tggtgtatgc cacgcgggtt tccagcggga tcgtggcact
      961 gatggtaacg atatttccct cctgcttggg aggttccgga agtatgtcgc gggtacagct
     1021 cgacagagtc agggcgagca ttgcaataat gaatagtttg ggtctcatgg ctgtcggatt
     1081 aagcagtcgg tgttaaacag cgtgtcattt cgtggatttg aattctttgt cgttgataat
     1141 caatgaggtt gtgaattgac acctaaagtt agaaagtttt tttgaaacga gaaaacaaac
     1201 gatgaacaag ttactccaaa ctattttatt atttttgtaa aaatattgcg aaatgaatta
     1261 tatcaaaacg gccatagagg gtcctgtgat tattgaaccg aaagtatttt acgaccacag
     1321 gggttatttt ttcgaatcat tttccctgag ggagtttgag gcaaatg
//
LOCUS       DAOSGL010000407         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_101872_length_1363_cov_4.282875, whole genome shotgun
            sequence.
ACCESSION   DAOSGL010000407 DAOSGL010000000
VERSION     DAOSGL010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101872_length_1363_cov_4.282875"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKY68_09640"
     CDS             complement(<1..636)
                     /locus_tag="PKY68_09640"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOH25328.1"
                     /translation="MDEKYPGKAQPVLVQKASGELQPFDSKKLELSLRNAGAKQATIQ
                     MILDDIQSWVYNGVTTKKIYARAFKMLRGERTAAALRYKLKQAIMEMGPTGYPFEQFI
                     GQIFQKQGYEIQVGVVVEGYSVTHEMDVIATKNGLQLLLECKYSLDQGRHVSVQVPLY
                     VRARVDDIVRKRKEHPLYKDLRFEGGVVTNTRFSADSIQYGRSAGLHLLAWD"
     gene            806..1291
                     /locus_tag="PKY68_09645"
     CDS             806..1291
                     /locus_tag="PKY68_09645"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOH25329.1"
                     /translation="MKKIVSLFACLFILSLTAGSLQAQNIADKIVGFYTTYDDNGEES
                     SQIQIFKTDDDKYHGKIVWLKEPLENGKPLLDSKNPNKALRNEPIIGLELITGLKYDP
                     KSQEWVNGSIYAPDNGKTYNCFIRFDSNDDTKLNLRGYVGKAWMGLGRTTTWIKGEPK
                     K"
BASE COUNT          381 a          340 c          299 g          343 t
ORIGIN      
        1 atcccaggcc agcaagtgca aaccggcaga ccgaccatat tgtatggaat cggcagaaaa
       61 acgggtattg gtcaccacgc ccccttcaaa ccgcaggtct ttgtacagag gatgctcttt
      121 gcgtttgcga acgatgtcgt ccactctggc gcgcacatag agcggcacct gcacgctgac
      181 atgccgtccc tggtccagac tgtatttaca ttcgagtagg agctgcagtc cgtttttggt
      241 ggcgatcacg tccatttcgt gcgttacgct ataaccctcg acaacgacac ctacctgaat
      301 ttcgtaaccc tgtttttgaa aaatctggcc gatgaattgc tcaaaaggat aaccggtggg
      361 gcccatttcc attatggcct gcttgagttt atagcgcaag gccgcagcgg ttcgttcgcc
      421 tctgagcatt ttgaatgccc gggcgtaaat tttcttggta gtgaccccat tatacaccca
      481 ggattggatg tcgtcgagaa tcatttgaat ggttgcttgt ttggcccccg catttcgcag
      541 ggaaagttcc agtttcttac tatcaaaggg ttgtaactct cccgaagctt tttgaacaag
      601 cacaggttgt gcttttcccg ggtacttttc gtccattacg cgactgattt tgaaccatca
      661 aaagtaaaaa cttttttcga cttttattgc tcttatcaaa aaaggcttat ttttgtaaag
      721 gcacgaaaac ctggagaaca agggtgtttt cctggttgaa acgcaacata ttcttgttta
      781 tcaattgatt ataaaaacca gacttatgaa aaagattgtt tccctttttg cctgcctctt
      841 tatattgagt ctgacggccg gcagcctgca agcacaaaac atagccgaca aaattgtcgg
      901 tttctatacc acctacgacg acaatggcga agaaagttca caaatacaaa ttttcaaaac
      961 cgacgacgac aaataccacg gcaaaattgt ctggctcaaa gagccccttg aaaacggcaa
     1021 acccctactg gacagcaaaa atcccaacaa ggccctgaga aatgaaccga ttatagggct
     1081 ggaactaatc acaggcctta aatacgatcc caaatcgcaa gaatgggtca atggcagtat
     1141 ctatgccccc gacaacggca aaacctacaa ttgctttata agattcgaca gcaacgacga
     1201 caccaaactc aacctccgcg gatacgtagg caaagcgtgg atgggtctgg gcaggacaac
     1261 cacctggata aagggcgagc cgaagaaata aaactccaca atctgtccgg tgacaaatcc
     1321 tttgtttcgg gcccgataac ttagcttgta ccatcggagc gct
//
LOCUS       DAOSGL010000408         1326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_106255_length_1326_cov_4.226593, whole genome shotgun
            sequence.
ACCESSION   DAOSGL010000408 DAOSGL010000000
VERSION     DAOSGL010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106255_length_1326_cov_4.226593"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKY68_09650"
     CDS             <1..354
                     /locus_tag="PKY68_09650"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HOH25330.1"
                     /translation="PEPRAFIEYTDENNITRKYRGGEPFAKARIMDAPGVIAALDDDL
                     LDVPFNVLSFETLIYDSMGNTNVEISEGANFSNRQKSQIRRLTRGQRFFIARIKVVGP
                     DKIEQTLSPMEIIIN"
     gene            372..>1326
                     /gene="gldN"
                     /locus_tag="PKY68_09655"
     CDS             372..>1326
                     /gene="gldN"
                     /locus_tag="PKY68_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOH25331.1"
                     /translation="MKKIALFVFALFSSLIIYAQLAPGSAATARPGTAAQSRPEQKSS
                     EQVPSDLPGLSLEKNKLPKAVSIQADNILWQRDVYRMLDLTKEKNAPLYHPAQPDGQR
                     KNLFSMLFELVASNQLAVYEYMDKKEVFSDEYKVNMEELLNRFWIPFESTPDPKNPTL
                     MVYTVDEADIPSQEVTLYYVKESYFLDQRNSSIRMKTIAFCPVLVREDEMGEIRRYPL
                     FWVAFDTAREALSRQTVSTNKYNSVNRISLYDFFVQHQYQGDIYRVSNLMDQNIMDYC
                     STPEEIAAEQERLEQELKDITATLWSAHKKEPASKGRKSSAN"
BASE COUNT          397 a          349 c          278 g          302 t
ORIGIN      
        1 cccgaacccc gtgctttcat cgaatatacc gacgaaaaca atattacccg taaataccgg
       61 ggcggcgaac cctttgcaaa agctcggatt atggatgctc ccggtgtcat agcagccctc
      121 gacgacgacc tgcttgatgt accttttaac gtcctgtctt ttgaaaccct tatttacgac
      181 tccatgggaa ataccaacgt agaaatctcc gagggagcaa atttctcgaa ccgacaaaaa
      241 tcacagattc gccgtctgac ccggggtcaa cgcttcttta tcgcccgcat caaggtagtg
      301 ggccccgata aaatagagca aaccctttcg cccatggaaa taataatcaa ctaagaaaat
      361 tcagcagcat tatgaaaaaa atagcccttt ttgtttttgc cctattcagc agcctgatta
      421 tttatgccca gctggcaccg ggctcggcag ccaccgccag gccgggcact gccgcccaaa
      481 gccgtccgga acaaaaaagt tccgaacagg tgccctcgga tcttcccggc ctgtcgctgg
      541 aaaaaaacaa acttcccaaa gctgtttcca tacaagcaga taatattctg tggcaaagag
      601 acgtttaccg catgctcgac ctgaccaaag aaaagaatgc tcccctatac catcccgctc
      661 aacccgacgg acaacgcaaa aatttgtttt cgatgctttt tgaactggtg gcctccaatc
      721 aattggccgt atacgaatac atggacaaaa aagaagtgtt ttcggacgaa tacaaagtca
      781 acatggaaga gttgctcaac cgtttctgga ttcccttcga aagcactccg gatcctaaaa
      841 acccgaccct tatggtttat acagtagacg aagcagacat cccaagccag gaagtaacgc
      901 tttattacgt aaaagagagc tattttctgg atcaacgcaa ctcttcgata cgtatgaaaa
      961 ccatcgcctt ttgtccggtg ttggtgagag aagacgaaat gggcgaaata agacgttacc
     1021 ccttgttctg ggtggctttc gacactgccc gcgaggccct gtccagacaa accgtaagta
     1081 ccaacaaata caacagcgtc aatcgcatca gcctttacga tttctttgtt cagcaccaat
     1141 atcagggaga tatttaccgg gtatccaatc ttatggatca aaatattatg gactactgct
     1201 ctacgcccga agaaattgcc gccgaacagg aaaggctgga acaagaatta aaagacatca
     1261 cggcaacgct ttggagtgcc cataaaaaag agcctgcttc caagggcagg aaatcttctg
     1321 cgaaca
//
LOCUS       DAOSGL010000409         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_110632_length_1292_cov_5.245756, whole genome shotgun
            sequence.
ACCESSION   DAOSGL010000409 DAOSGL010000000
VERSION     DAOSGL010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110632_length_1292_cov_5.245756"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1292
                     /locus_tag="PKY68_09660"
     CDS             <1..>1292
                     /locus_tag="PKY68_09660"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOH25332.1"
                     /translation="DLLTKYGSHTNIKAIAHHLSSSSGILRLKGLAGSATALSLAAVF
                     TGGKRDFLLVQDDAEQAAYLYHDLNQLLGRDRVYLFPSSFRRRIGKGLEDAGNIILRT
                     EVLNALQFSSREPLIVVSYPEALAEKVLSRQELKNNTLKIKVGEQVDNRFVIELLHEY
                     GFEQTDYVYEPGQFAQRGSLIDVYSWAYELPFRIDFYGNEIESIRSFEVESQLSKSMF
                     SEVNIILHQQNTKGGHLVSLLDYLDEKTVFGFSGLAYCLNRIELIINDSSAFNAAHLL
                     TEVGELTKAIQSNNCLEYGSNTSGEQQAEIKFNCSHQKKFSKRFDLLAEHLQKHINDG
                     YQLCILSNNPKQLDRITAIFEERKQKIAYSPILQTLHEGFCDHDLKLLCYTDHQLFDR
                     FHRYSLRSDRVRNGKLALSMKELLQFQFGDYVVHIDHG"
BASE COUNT          383 a          294 c          272 g          343 t
ORIGIN      
        1 aggacttact tacaaaatac ggctcacata ccaacattaa agcgattgcc catcatctga
       61 gctcatcttc aggcatactg cgactcaaag gcctggccgg ttcggccacg gctctgtcac
      121 tggctgcggt ttttaccggc gggaaacgtg attttctgct tgtacaggac gatgccgaac
      181 aagctgctta cctttaccac gatctgaatc aattgctggg ccgggacagg gtgtatttat
      241 tcccctcaag tttccggcgc agaatcggaa aaggtttgga agacgccggc aatattattc
      301 tcagaaccga agtcctgaat gctttacagt tttcttccag agagcctctg attgtagtca
      361 gttatcccga agccctggcc gagaaagtcc tctcacgtca agagctcaaa aacaatactc
      421 ttaaaatcaa agtcggagag caggtcgaca accgctttgt aatcgaattg ctccatgaat
      481 atggttttga acaaaccgat tacgtttatg agccggggca atttgcacaa agaggaagtt
      541 tgatcgatgt ctattcatgg gcttatgaac taccttttcg tatcgatttc tacgggaatg
      601 aaatcgaaag cattcgcagt tttgaggtag aaagccaatt gtccaaatct atgttttcgg
      661 aggttaacat catacttcat cagcaaaaca ccaaaggagg acacttagtt tctctacttg
      721 attacctgga cgaaaaaacc gtttttggtt tttcggggct ggcgtactgc ctcaatagaa
      781 tagaattgat aatcaacgac tcttctgctt ttaatgcagc tcatttattg acggaagtcg
      841 gcgaactgac aaaagctatc caatcaaaca attgcctcga atatggcagc aacacaagtg
      901 gtgaacaaca ggccgaaatt aaattcaact gcagtcatca aaaaaaattt tccaaacgct
      961 ttgacttact ggccgagcat ttacaaaaac acataaacga tggctatcaa ttgtgcattt
     1021 tgagcaataa ccccaaacaa ttggaccgca tcacggctat tttcgaagaa agaaaacaaa
     1081 agatagccta tagccccata ttgcaaaccc tacacgaggg cttttgcgat cacgatctca
     1141 aattgctttg ttataccgat catcagttgt ttgaccgctt tcatagatac agcttgcgct
     1201 ccgaccgggt acgcaacggg aaactggctt tgagcatgaa agagttactg caattccaat
     1261 ttggagatta tgtggtacac atcgatcacg gt
//
LOCUS       DAOSGL010000410         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_123934_length_1201_cov_2.513962, whole genome shotgun
            sequence.
ACCESSION   DAOSGL010000410 DAOSGL010000000
VERSION     DAOSGL010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123934_length_1201_cov_2.513962"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKY68_09665"
     CDS             complement(<1..299)
                     /locus_tag="PKY68_09665"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOH25333.1"
                     /translation="MDRIKKLLKWIVPLLLGVLLFAWIYKRMDWISIKEIFQLGLRYE
                     WMLAFLLIFVSGMVARGLRWQQLLEPVCPQADTTSTTLAVFVAYAANLIFPRIGEV"
     gene            465..>1201
                     /gene="rsmA"
                     /locus_tag="PKY68_09670"
     CDS             465..>1201
                     /gene="rsmA"
                     /locus_tag="PKY68_09670"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOH25334.1"
                     /translation="MTIVRPKKALGQHFLRDLSVALRIAATLDDCKGMPVLEVGPGTG
                     VLTRFLLDKGHDLRVVEIDGESVAYLNEHFPELAGGRILAEDFLKTDLNGLYRGQPFC
                     LIGNYPYNISSQIFFKVLENKDQVQHCVGMLQKEVAERICSPVGKKSYGILSVFLQAY
                     YDIDYLFTVSEKVFEPPPKVKSAVIRMNRNNNLKLACDENLFRKVVKTAFNQRRKTLR
                     NSLKPLLGKEAPFYTEAIFDKRPEQLSV"
BASE COUNT          336 a          266 c          264 g          335 t
ORIGIN      
        1 acttcgccga ttctcggaaa gatcaggttg gcagcataag caacaaaaac ggccagtgta
       61 gtcgatgtag tatctgcttg ggggcaaacc ggctccagta attgttgcca gcgcaatccc
      121 ctcgccacca tgccgctcac aaaaatgagc aaaaaggcca acatccattc gtaacgcagg
      181 ccaagttgaa aaatctcttt aatggagatc cagtccatgc gtttatatat ccaggcaaaa
      241 agcaaaactc ccagtaataa agggactatc catttcagga gcttttttat tctatccatc
      301 gtactacaat tcaataaaaa tgcttatttt tgtgaccgcg gcaaaaatac gtatattttg
      361 ttgcctgatt tcattctctg gtgacaaaat tacatcaacc gtaaaataaa ggatcggttt
      421 gctgttgctc cggtttatgt ttcatttttt ccttaaagct gtggatgacg attgtaagac
      481 caaagaaagc actgggacag catttcctca gggacctgtc tgttgccctt cgtatcgcag
      541 ctacgctgga tgactgcaag ggaatgcccg tactcgaagt cggtccgggt acaggtgtat
      601 taactcgttt tttactcgat aaaggccacg acctcagggt ggtggaaatc gatggggaat
      661 ctgtagctta tctgaacgag cattttcccg aattggccgg cggccgtatt ttagccgaag
      721 attttctaaa aaccgatcta aacggtcttt accggggcca gcctttctgc ctgataggca
      781 attatcctta caacatctct tcgcagattt tttttaaggt actcgaaaac aaagatcagg
      841 tgcagcattg cgtgggcatg cttcaaaagg aggtggccga gcgcatatgt tctccggtcg
      901 gcaagaaaag ctacggtatt ctgagtgtct ttcttcaagc ttattacgat atcgactatt
      961 tatttactgt gagcgagaag gtttttgaac ctcctccaaa agtaaaatct gctgttattc
     1021 gtatgaatcg taacaacaac ctaaagctgg cttgcgacga aaatttgttt agaaaggtgg
     1081 ttaaaacagc tttcaatcag cgcagaaaaa ccttgcgcaa ttccttaaag ccattgctgg
     1141 ggaaagaagc ccctttttat actgaagcta ttttcgacaa gcgtcccgag cagctcagtg
     1201 t
//
LOCUS       DAOSGL010000411         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_125923_length_1188_cov_3.910856, whole genome shotgun
            sequence.
ACCESSION   DAOSGL010000411 DAOSGL010000000
VERSION     DAOSGL010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125923_length_1188_cov_3.910856"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKY68_09675"
     CDS             <1..223
                     /locus_tag="PKY68_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOH25335.1"
                     /translation="GRFVSIAAASILAKTYRDDYMQHLHKQYPQYAWNKNRGYPTAEH
                     RRAIADFGISPFHRKTFRLLPSEQQLSLF"
     gene            226..>1188
                     /locus_tag="PKY68_09680"
     CDS             226..>1188
                     /locus_tag="PKY68_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOH25336.1"
                     /translation="MAAIKDLTRGPIGKQLFYLSLPIISTSFIQMAYSLTDMAWVGRL
                     GPESVAAVGAVGILVWLTNSVSLLNKVGAEISVAQAIGAQNLDKARCYASHNVSISLI
                     ISLIWAGFLFAFSYPLLGIYKIEAAIELEAVTYLRIVLFGFPFLFLSATFTGIYNAAG
                     LSKIPFYINSIGLILNLVLDPVFIFALDMGTAGAALATLISQVVVCLLFFARIKLRDR
                     LLGSFPFFVRLQPDCVRKIVNLGLPVTLFNGFFAIINLLMGRISSIFGGHIGLMTLTA
                     GGQIEGLAWNTSQGFSTGLSAFVAQNYAAGKKSRILRAYRSGLAI"
BASE COUNT          277 a          292 c          271 g          348 t
ORIGIN      
        1 cggaagattt gtatccattg ctgcagcctc catactggcc aaaacataca gagacgatta
       61 tatgcagcat ttacacaagc aataccctca atatgcctgg aacaaaaacc gaggctaccc
      121 taccgcagaa catcgccggg ccattgcaga tttcggaatc agccctttcc atcgaaagac
      181 attcaggctg ttgccgtcag aacagcaatt aagtctattt taagtatggc tgcaataaaa
      241 gacttaaccc ggggacccat cggtaaacag cttttttatc tctcccttcc cattatcagc
      301 acttctttta ttcaaatggc ttacagcctg acggatatgg cctgggttgg ccgtctgggt
      361 cccgaatcgg tagctgccgt cggagctgtc ggcatcttag tctggcttac caattcggtc
      421 tctctgttga acaaagtggg ggccgaaatc agcgtggctc aggccatagg agctcagaat
      481 ttggacaagg cccgttgcta cgcttcgcac aacgtcagca tctcgctaat tatttccctg
      541 atttgggccg gttttttgtt tgcattttcc tatcccctgc tgggtattta taaaatagaa
      601 gccgctattg agctggaagc cgtaacttac ttaagaattg tgcttttcgg gtttcctttt
      661 ctgtttttgt ctgccacctt caccggaatc tacaacgcgg ccggactgag caaaattccc
      721 ttttacatca acagcatcgg tttgatttta aacttagtac tcgatcctgt atttattttt
      781 gccttagata tgggtacggc tggagcagcc ttagcaacat tgatttcgca ggtggtggtc
      841 tgtctcttat ttttcgcccg gataaagctt agggacaggc tattaggaag ttttcctttt
      901 tttgtccggc tgcaaccgga ctgcgtccgt aaaattgtaa acctggggct gcccgtaaca
      961 cttttcaacg gcttttttgc cattatcaat ttattaatgg gacggatctc atcgatcttc
     1021 ggaggacata ttgggctgat gactcttact gccggaggcc aaatcgaggg cctggcctgg
     1081 aatacctcgc aaggtttcag caccggtctg agtgcttttg ttgcccaaaa ctacgctgcc
     1141 ggtaaaaaat cgagaatttt acgcgcatat cgctcggggc tggccata
//
LOCUS       DAOSGL010000412         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_157062_length_1031_cov_4.648566, whole genome shotgun
            sequence.
ACCESSION   DAOSGL010000412 DAOSGL010000000
VERSION     DAOSGL010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157062_length_1031_cov_4.648566"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKY68_09685"
     CDS             complement(<1..238)
                     /locus_tag="PKY68_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25337.1"
                     /translation="MPFAFSSGSSGESEIIFAGYGFSINEDSLKWNDYKGIDVREKWV
                     LVLRADPDTDKPDSPFIPYSSDRGKALLARDLGAA"
     gene            219..344
                     /locus_tag="PKY68_09690"
                     /pseudo
     CDS             219..344
                     /locus_tag="PKY68_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
     gene            complement(535..>1031)
                     /locus_tag="PKY68_09695"
     CDS             complement(535..>1031)
                     /locus_tag="PKY68_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH25338.1"
                     /translation="MQRPGRLSRMWNKYLPWSITGILSPSAYPVFSSMAEVPKAVWRN
                     RRLVVEKFCPEREGDHYCLRQWIFLGDRGVSQRLVSLNPIVKFPNEVHLEYGPPVPES
                     LREMRSRLGFDYGKFDYVVVDGEVILLDANRTPALGGSDGWTQNVAAELAGGLPSFLR
                     GLDD"
BASE COUNT          259 a          284 c          243 g          245 t
ORIGIN      
        1 ctgctgctcc gaggtctctg gcaagaagtg ccttgccccg gtcgctgctg taaggtatga
       61 acggactgtc cggtttatcg gtatccggat ctgccctgag taccaatacc catttttctc
      121 ttacatcaat tcctttataa tcgttccatt taagactgtc ttcattgatt gagaatccgt
      181 accctgcaaa gattatttct gattcacccg aagagcctga actgaaggca aaaggcatac
      241 gtctgattga tgaaaagccc cgctacggcg cggggggcgc aaaaatagcc tttatccatc
      301 ctaaagcaac aggcggtgtt ttgctggagc tgagccagag gtaaaactac caaacggcag
      361 acaatgccaa cttcagaagt tttactttga tcaaggggaa acaaagagag tccctggccg
      421 tgatgttgtc tgtccaggct ttacaggacg atgcttctga cgaacttcaa cctcaaagcg
      481 gtttaccaaa aaacaaacca ccggcctgac caaaccttcc aaccctctaa tcttctaatc
      541 atctaatcct ctcagaaacg agggaagccc tccggccagt tcggcggcta cgttttgcgt
      601 ccacccatct gagccgccca gggcaggtgt gcggttggcg tcgagcagga tgacttcgcc
      661 gtccacaact acatagtcga actttccata atcaaatcca agtcggctgc gcatttcccg
      721 caggctctcc ggaacaggcg ggccgtattc gagatggact tcgttgggaa atttgacaat
      781 tgggtttaaa gaaacgaggc gctggctcac gccgcgatct cccagaaata tccactgccg
      841 caggcaataa tgatcccctt cacgctcggg acaaaacttc tccacaacca gccgtcggtt
      901 gcgccaaact gctttgggga cctctgccat agagctgaaa acaggatagg cagacggcga
      961 cagtattccc gttatactcc agggaaggta tttgttccac attctgctta gtcgccccgg
     1021 acgttgcatt t
//
LOCUS       DAOSGL010000413         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1754
            NODE_160516_length_1017_cov_3.328482, whole genome shotgun
            sequence.
ACCESSION   DAOSGL010000413 DAOSGL010000000
VERSION     DAOSGL010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863283
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:58:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,941
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 8 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 1 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160516_length_1017_cov_3.328482"
                     /isolate="AMR_MDS_1754"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKY68_09700"
     CDS             complement(<1..456)
                     /locus_tag="PKY68_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOH25339.1"
                     /translation="MFDDIRAYTDQELRPTLERLWADPEFTSVVAALPFIDSVEALKQ
                     ASLQCTSINQLHKNVIHRLLTLLAEKTCASLQGEGFEKINPEKAYIYMSNHRDIVLDS
                     SFLSVILLNNGLESCETGIGDNLLIRPWITDLVRANKSFVVKRNLSVREQ"
     gene            complement(449..>1017)
                     /locus_tag="PKY68_09705"
     CDS             complement(449..>1017)
                     /locus_tag="PKY68_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOH25340.1"
                     /translation="NKIVAETGLPCFVKPNLGGSSFGTSKIKMASELSRAIDLAYGEC
                     NEVLVEGFLPGTELTNGCYKTTKGLVCLPVTEVISKNEFFDYEAKYNPDKAKEITPAR
                     IPESMRDEIQHTTARIYKLLDCKGIIRVDYILCGSALYLLEVNTTPGMTATSFIPQQV
                     KAAGMTMTEVMEQVIEDVLFTTKTTGHV"
BASE COUNT          235 a          220 c          259 g          303 t
ORIGIN      
        1 ttgttctctt accgaaaggt tgcgctttac cacaaagctt ttgttggctc tgacaaggtc
       61 ggtgatccag gggcgtatga gcaggttgtc tccaattccc gtctcacagc tctcaaggcc
      121 gttgttaagc aggatgacac ttaaaaagct ggaatccagc acaatatccc tatggttgga
      181 catgtaaata taagcttttt cgggatttat tttctcaaag ccttcccctt gaaggctggc
      241 acaggtcttt tcggccagca gagtcaacaa tctatgaata acgtttttat gtaattgatt
      301 tatgcttgtg cattgcaacg aagcttgttt aagtgcttcg acggaatcga taaaaggaag
      361 ggctgcaacg accgaggtga attcagggtc tgcccaaagc cgttccagcg tcgggcgaag
      421 ttcctggtcg gtataggctc tgatatcgtc aaacatggcc ggtggttttt gttgtgaaaa
      481 gaacatcttc gattacctgc tccattacct ctgtcatggt catgcctgca gcttttacct
      541 gttggggaat gaagctggtg gccgtcatgc cgggagtggt gtttacttcg agcagataaa
      601 gtgctgagcc gcagagaatg taatcaactc tgattatgcc cttgcagtcg agcagtttgt
      661 agattcgggc ggtggtatgc tgtatttcgt ctcgcatact ttcgggaata cgggcaggag
      721 tgatttcttt ggctttgtcc ggattgtatt tggcttcgta atcgaaaaat tcgtttttgc
      781 tgataacttc ggtaacgggc agacaaacca aacccttagt cgttttatag cagccgttag
      841 tcagttcggt gcctggtaaa aagccttcaa ccagcacttc gttgcattcg ccgtaggcca
      901 ggtctatggc ccgggaaagt tcggaagcca ttttaatttt gctggtacca aagctcgatc
      961 cacccaggtt gggtttaaca aaacaaggca ggccggtttc tgctactatt ttgtttg
//
